Pngl cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pngl
Entrez Gene ID	35527
Full Name	PNGase-like
Synonyms	CG7865,Dmel\CG7865,PNGase,png1
General protein information	Preferred Names PNGase-like Names CG7865 gene product from transcript CG7865-RC CG7865-PA CG7865-PC PNGase Pngl-PA Pngl-PC
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 42A7-42A8

mRNA and Protein(s)

mRNA	Protein	Name
NM_136348.3	NP_610192.1	PNGase-like, isoform A
NM_165454.3	NP_724444.3	PNGase-like, isoform C

Pathways from BioSystems

Homologies

Bos taurus (cattle)	NGLY1	NP_001179716.1
Rattus norvegicus (Norway rat)	Ngly1	NP_001014158.1
Neurospora crassa	NCU00651	XP_965791.1
Mus musculus (house mouse)	Ngly1	NP_067479.2
Danio rerio (zebrafish)	ngly1	NP_001018437.1
Homo sapiens (human)	NGLY1	NP_060767.2
	NGLY1	XP_001092796.1
Canis lupus familiaris (dog)	NGLY1	XP_003639867.1
Gallus gallus (chicken)	NGLY1	NP_001026159.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007390	XP_308442.4
Caenorhabditis elegans (roundworm)	png-1	NP_492913.1
Saccharomyces cerevisiae (baker's yeast)	PNG1	NP_015229.1
Kluyveromyces lactis	KLLA0E24223g	XP_455043.1
Magnaporthe oryzae (rice blast fungus)	MGG_03598	XP_003716307.1
Pan troglodytes (chimpanzee)	NGLY1	XP_003309721.1
Schizosaccharomyces pombe (fission yeast)	SPBC1709.14	NP_595447.1
Drosophila melanogaster (fruit fly)	Pngl	NP_724444.3
Eremothecium gossypii	AGOS_ABR193W	NP_983142.1
Xenopus tropicalis (tropical clawed frog)	ngly1	XP_002932449.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Pngl was shown to be inevitable for the proper developmental transition and the biochemical properties other than deglycosylation activity is important for its biological function.
Title: Evidence for an essential deglycosylation-independent activity of PNGase in Drosophila melanogaster.

The following Pngl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pngl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa12417	NM_136348.3 Latest version!	Drosophila melanogaster PNGase-like (Pngl), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa12417	NM_165454.3 Latest version!	Drosophila melanogaster PNGase-like (Pngl), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pngl ( NM_136348.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610192.1 Drosophila melanogaster PNGase-like (Pngl), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa12417
Clone ID Related Accession (Same CDS sequence)	NM_136348.3 , NM_165454.3
Accession Version	NM_136348.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1896bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	PNGase-like, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_136348.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTGGACA TAAACTTGGA GTGCGTGCAC CAGATCGAAC CGAAGACCCG TTCTGCTGGT 
CAGCAGCAGC AGGAGCGAGG GTTAAAGGAA GCCTACCTGG AAGCGGTCCG CATTCTGTTA 
GTGCTGCTGG AGAACATCTT GGCGCAGCCG GAGAACTCCA TGTTCCGCAC CATACGGCAG 
GAAAACAAAG CTATTAAAGA AAAGCTGCTG TCCTTGCCCG GCTGTGAGAG GTTACTGGAA 
GCCATAGGCT TCGTACGCGC CCCCAGTTCC AACGCCTACA CCTTGCCCAC AGAGGTGTCA 
CTGCAGCAGG TGAAGAAGTA CAGAGATGCG CTGAGCGAGA GACGCACTGC CTGGCTTAAC 
GGCACAGTGT CAAAAAGTCC TCCTCAACAA AGCACCACCA GCACCACGCC CTTGTTCATC 
AAGCCTTCTG TGGAATATCG CCATAGAATC GCCTTCCCCC GTGTATTGCG CACCAATAAT 
AACTTTCTGC AGTCGCTAGA GCTTTATTCC GATGCAGTCA TGCAGTACGA GGATAACCTG 
CTGCTGGCTA CTGGCCGCAC TCTGATTCCT GTGGAAGAGC TCACCGAAAT GGCAAGCGAG 
AAACTGATAG ACATCCAAGA TCAAATTGCG TCGGGTGAGC GCCAGGAGAA GGAGCCATGT 
GTTCGCGACT TGCTGCTTGT GGAGCTGGTA AACTGGTTCA ACACGCAGTT CTTCCAGTGG 
GTAAACAATA TACCTTGCCG CGTGTGTGGG AGTGAAGAGA GCAGGCTGCG CCGCACTGAA 
CGGGAAGGTG ATATAAGGGT GGAAGTCACC GTTTGCTGCG GCCAGGAGAG CAAGTTCTAC 
CGCTATAACG ACATTTCCCA GCTCCTAGTT TCCCGTAAGG GCCGTTGCGG AGAATATGCC 
AATTGCTTCA CATTCCTCTG TCGCGCCCTC GACTACGACG CCCGTATTGT CCACTCGCAT 
TTTGATCACG TCTGGACCGA GGTGTACTCC GAGGCCCAGA TGCGTTGGCT CCATGTTGAC 
CCCTCCGAGA ACGTTATAGA TTCTCCGCTG ATGTATCAGC ATGGCTGGAA ACGTCACATC 
GACTACATTC TTGCATACTC TAGGGACGAC ATCCAAGATG TGACTTGGCG CTATACTAAT 
GACCACCAGA AGATTCTACA CCTACGAAAG CTGTGCGGGG AAAAAGAAAT GGTGCAAACG 
CTGGACGCGA TTCGAGCAAA GCGGCGGCAA AATTGCACCG CCGATCGAAA GTTATTCCTC 
AGCCAGCGCA ATATGTACGA GGTCATCGGC TTGACGCTAG AGCGTAAGCC GACAGAAAAC 
GAGCTGAAGG GTCGGAGTTC GGGAAGTCTT TCTTGGCGGC AGTCACGTGG AGAGCATACT 
TTTACCAATA TATTCGTTTT CAATCTAAGT GCAACCGAAC TACAAAAAAG GCAACTTAAT 
GTGCGTTACA GCTGTGCCAC CGATACATAC GAACGATATG CCAAAGAAGG AGAACACATC 
ACTATTTTGG ATAGCTATAA GACATGGCAA AAAGCGCAAT TTTCCTCAAA GAACATATTT 
CGGAAAGTGG AACGCGATTG GAAAATGGCA TATTTGGCTA GACTGGAGGA TACCGATTGC 
GGAGAAATTG CCTGGACGTT TGATTTCAGC AAGACCAATT TAAAGGTTAA GTCTTACAAC 
TTAGTTTTCG AGACAAAAAC TTTTGGCGAC GGAAAAATCA GCGTGACTGT CGATGCCACC 
GACGGAAGCG CTTCGGTTGA AAATGCCACT GGATTCAAAA TAGTGGCCAA ACTTACGGGT 
GGCAAGGGCG ATGTAGCATG GCAACACACA CAACTGTTCA GGCAGAGTCT TAATTCAAGA 
GACTATCCCT TCGATCTGCA AGTGCAACTA CATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610192.1
CDS	95..1990
Translation	MVDINLECVHQIEPKTRSAGQQQQERGLKEAYLEAVRILLVLLENILAQPENSMFRTIRQENKAIKEKLLSLPGCERLLEAIGFVRAPSSNAYTLPTEVSLQQVKKYRDALSERRTAWLNGTVSKSPPQQSTTSTTPLFIKPSVEYRHRIAFPRVLRTNNNFLQSLELYSDAVMQYEDNLLLATGRTLIPVEELTEMASEKLIDIQDQIASGERQEKEPCVRDLLLVELVNWFNTQFFQWVNNIPCRVCGSEESRLRRTEREGDIRVEVTVCCGQESKFYRYNDISQLLVSRKGRCGEYANCFTFLCRALDYDARIVHSHFDHVWTEVYSEAQMRWLHVDPSENVIDSPLMYQHGWKRHIDYILAYSRDDIQDVTWRYTNDHQKILHLRKLCGEKEMVQTLDAIRAKRRQNCTADRKLFLSQRNMYEVIGLTLERKPTENELKGRSSGSLSWRQSRGEHTFTNIFVFNLSATELQKRQLNVRYSCATDTYERYAKEGEHITILDSYKTWQKAQFSSKNIFRKVERDWKMAYLARLEDTDCGEIAWTFDFSKTNLKVKSYNLVFETKTFGDGKISVTVDATDGSASVENATGFKIVAKLTGGKGDVAWQHTQLFRQSLNSRDYPFDLQVQLH

Target ORF information:

RefSeq Version	NM_136348.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster PNGase-like (Pngl), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136348.3

ORF Insert Sequence:

ATGGTGGACA TAAACTTGGA GTGCGTGCAC CAGATCGAAC CGAAGACCCG TTCTGCTGGT 
CAGCAGCAGC AGGAGCGAGG GTTAAAGGAA GCCTACCTGG AAGCGGTCCG CATTCTGTTA 
GTGCTGCTGG AGAACATCTT GGCGCAGCCG GAGAACTCCA TGTTCCGCAC CATACGGCAG 
GAAAACAAAG CTATTAAAGA AAAGCTGCTG TCCTTGCCCG GCTGTGAGAG GTTACTGGAA 
GCCATAGGCT TCGTACGCGC CCCCAGTTCC AACGCCTACA CCTTGCCCAC AGAGGTGTCA 
CTGCAGCAGG TGAAGAAGTA CAGAGATGCG CTGAGCGAGA GACGCACTGC CTGGCTTAAC 
GGCACAGTGT CAAAAAGTCC TCCTCAACAA AGCACCACCA GCACCACGCC CTTGTTCATC 
AAGCCTTCTG TGGAATATCG CCATAGAATC GCCTTCCCCC GTGTATTGCG CACCAATAAT 
AACTTTCTGC AGTCGCTAGA GCTTTATTCC GATGCAGTCA TGCAGTACGA GGATAACCTG 
CTGCTGGCTA CTGGCCGCAC TCTGATTCCT GTGGAAGAGC TCACCGAAAT GGCAAGCGAG 
AAACTGATAG ACATCCAAGA TCAAATTGCG TCGGGTGAGC GCCAGGAGAA GGAGCCATGT 
GTTCGCGACT TGCTGCTTGT GGAGCTGGTA AACTGGTTCA ACACGCAGTT CTTCCAGTGG 
GTAAACAATA TACCTTGCCG CGTGTGTGGG AGTGAAGAGA GCAGGCTGCG CCGCACTGAA 
CGGGAAGGTG ATATAAGGGT GGAAGTCACC GTTTGCTGCG GCCAGGAGAG CAAGTTCTAC 
CGCTATAACG ACATTTCCCA GCTCCTAGTT TCCCGTAAGG GCCGTTGCGG AGAATATGCC 
AATTGCTTCA CATTCCTCTG TCGCGCCCTC GACTACGACG CCCGTATTGT CCACTCGCAT 
TTTGATCACG TCTGGACCGA GGTGTACTCC GAGGCCCAGA TGCGTTGGCT CCATGTTGAC 
CCCTCCGAGA ACGTTATAGA TTCTCCGCTG ATGTATCAGC ATGGCTGGAA ACGTCACATC 
GACTACATTC TTGCATACTC TAGGGACGAC ATCCAAGATG TGACTTGGCG CTATACTAAT 
GACCACCAGA AGATTCTACA CCTACGAAAG CTGTGCGGGG AAAAAGAAAT GGTGCAAACG 
CTGGACGCGA TTCGAGCAAA GCGGCGGCAA AATTGCACCG CCGATCGAAA GTTATTCCTC 
AGCCAGCGCA ATATGTACGA GGTCATCGGC TTGACGCTAG AGCGTAAGCC GACAGAAAAC 
GAGCTGAAGG GTCGGAGTTC GGGAAGTCTT TCTTGGCGGC AGTCACGTGG AGAGCATACT 
TTTACCAATA TATTCGTTTT CAATCTAAGT GCAACCGAAC TACAAAAAAG GCAACTTAAT 
GTGCGTTACA GCTGTGCCAC CGATACATAC GAACGATATG CCAAAGAAGG AGAACACATC 
ACTATTTTGG ATAGCTATAA GACATGGCAA AAAGCGCAAT TTTCCTCAAA GAACATATTT 
CGGAAAGTGG AACGCGATTG GAAAATGGCA TATTTGGCTA GACTGGAGGA TACCGATTGC 
GGAGAAATTG CCTGGACGTT TGATTTCAGC AAGACCAATT TAAAGGTTAA GTCTTACAAC 
TTAGTTTTCG AGACAAAAAC TTTTGGCGAC GGAAAAATCA GCGTGACTGT CGATGCCACC 
GACGGAAGCG CTTCGGTTGA AAATGCCACT GGATTCAAAA TAGTGGCCAA ACTTACGGGT 
GGCAAGGGCG ATGTAGCATG GCAACACACA CAACTGTTCA GGCAGAGTCT TAATTCAAGA 
GACTATCCCT TCGATCTGCA AGTGCAACTA CATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pngl ( NM_165454.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724444.3 Drosophila melanogaster PNGase-like (Pngl), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa12417
Clone ID Related Accession (Same CDS sequence)	NM_136348.3 , NM_165454.3
Accession Version	NM_165454.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1896bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	PNGase-like, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_165454.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTGGACA TAAACTTGGA GTGCGTGCAC CAGATCGAAC CGAAGACCCG TTCTGCTGGT 
CAGCAGCAGC AGGAGCGAGG GTTAAAGGAA GCCTACCTGG AAGCGGTCCG CATTCTGTTA 
GTGCTGCTGG AGAACATCTT GGCGCAGCCG GAGAACTCCA TGTTCCGCAC CATACGGCAG 
GAAAACAAAG CTATTAAAGA AAAGCTGCTG TCCTTGCCCG GCTGTGAGAG GTTACTGGAA 
GCCATAGGCT TCGTACGCGC CCCCAGTTCC AACGCCTACA CCTTGCCCAC AGAGGTGTCA 
CTGCAGCAGG TGAAGAAGTA CAGAGATGCG CTGAGCGAGA GACGCACTGC CTGGCTTAAC 
GGCACAGTGT CAAAAAGTCC TCCTCAACAA AGCACCACCA GCACCACGCC CTTGTTCATC 
AAGCCTTCTG TGGAATATCG CCATAGAATC GCCTTCCCCC GTGTATTGCG CACCAATAAT 
AACTTTCTGC AGTCGCTAGA GCTTTATTCC GATGCAGTCA TGCAGTACGA GGATAACCTG 
CTGCTGGCTA CTGGCCGCAC TCTGATTCCT GTGGAAGAGC TCACCGAAAT GGCAAGCGAG 
AAACTGATAG ACATCCAAGA TCAAATTGCG TCGGGTGAGC GCCAGGAGAA GGAGCCATGT 
GTTCGCGACT TGCTGCTTGT GGAGCTGGTA AACTGGTTCA ACACGCAGTT CTTCCAGTGG 
GTAAACAATA TACCTTGCCG CGTGTGTGGG AGTGAAGAGA GCAGGCTGCG CCGCACTGAA 
CGGGAAGGTG ATATAAGGGT GGAAGTCACC GTTTGCTGCG GCCAGGAGAG CAAGTTCTAC 
CGCTATAACG ACATTTCCCA GCTCCTAGTT TCCCGTAAGG GCCGTTGCGG AGAATATGCC 
AATTGCTTCA CATTCCTCTG TCGCGCCCTC GACTACGACG CCCGTATTGT CCACTCGCAT 
TTTGATCACG TCTGGACCGA GGTGTACTCC GAGGCCCAGA TGCGTTGGCT CCATGTTGAC 
CCCTCCGAGA ACGTTATAGA TTCTCCGCTG ATGTATCAGC ATGGCTGGAA ACGTCACATC 
GACTACATTC TTGCATACTC TAGGGACGAC ATCCAAGATG TGACTTGGCG CTATACTAAT 
GACCACCAGA AGATTCTACA CCTACGAAAG CTGTGCGGGG AAAAAGAAAT GGTGCAAACG 
CTGGACGCGA TTCGAGCAAA GCGGCGGCAA AATTGCACCG CCGATCGAAA GTTATTCCTC 
AGCCAGCGCA ATATGTACGA GGTCATCGGC TTGACGCTAG AGCGTAAGCC GACAGAAAAC 
GAGCTGAAGG GTCGGAGTTC GGGAAGTCTT TCTTGGCGGC AGTCACGTGG AGAGCATACT 
TTTACCAATA TATTCGTTTT CAATCTAAGT GCAACCGAAC TACAAAAAAG GCAACTTAAT 
GTGCGTTACA GCTGTGCCAC CGATACATAC GAACGATATG CCAAAGAAGG AGAACACATC 
ACTATTTTGG ATAGCTATAA GACATGGCAA AAAGCGCAAT TTTCCTCAAA GAACATATTT 
CGGAAAGTGG AACGCGATTG GAAAATGGCA TATTTGGCTA GACTGGAGGA TACCGATTGC 
GGAGAAATTG CCTGGACGTT TGATTTCAGC AAGACCAATT TAAAGGTTAA GTCTTACAAC 
TTAGTTTTCG AGACAAAAAC TTTTGGCGAC GGAAAAATCA GCGTGACTGT CGATGCCACC 
GACGGAAGCG CTTCGGTTGA AAATGCCACT GGATTCAAAA TAGTGGCCAA ACTTACGGGT 
GGCAAGGGCG ATGTAGCATG GCAACACACA CAACTGTTCA GGCAGAGTCT TAATTCAAGA 
GACTATCCCT TCGATCTGCA AGTGCAACTA CATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724444.3
CDS	95..1990
Translation	MVDINLECVHQIEPKTRSAGQQQQERGLKEAYLEAVRILLVLLENILAQPENSMFRTIRQENKAIKEKLLSLPGCERLLEAIGFVRAPSSNAYTLPTEVSLQQVKKYRDALSERRTAWLNGTVSKSPPQQSTTSTTPLFIKPSVEYRHRIAFPRVLRTNNNFLQSLELYSDAVMQYEDNLLLATGRTLIPVEELTEMASEKLIDIQDQIASGERQEKEPCVRDLLLVELVNWFNTQFFQWVNNIPCRVCGSEESRLRRTEREGDIRVEVTVCCGQESKFYRYNDISQLLVSRKGRCGEYANCFTFLCRALDYDARIVHSHFDHVWTEVYSEAQMRWLHVDPSENVIDSPLMYQHGWKRHIDYILAYSRDDIQDVTWRYTNDHQKILHLRKLCGEKEMVQTLDAIRAKRRQNCTADRKLFLSQRNMYEVIGLTLERKPTENELKGRSSGSLSWRQSRGEHTFTNIFVFNLSATELQKRQLNVRYSCATDTYERYAKEGEHITILDSYKTWQKAQFSSKNIFRKVERDWKMAYLARLEDTDCGEIAWTFDFSKTNLKVKSYNLVFETKTFGDGKISVTVDATDGSASVENATGFKIVAKLTGGKGDVAWQHTQLFRQSLNSRDYPFDLQVQLH

Target ORF information:

RefSeq Version	NM_165454.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster PNGase-like (Pngl), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165454.3

ORF Insert Sequence:

ATGGTGGACA TAAACTTGGA GTGCGTGCAC CAGATCGAAC CGAAGACCCG TTCTGCTGGT 
CAGCAGCAGC AGGAGCGAGG GTTAAAGGAA GCCTACCTGG AAGCGGTCCG CATTCTGTTA 
GTGCTGCTGG AGAACATCTT GGCGCAGCCG GAGAACTCCA TGTTCCGCAC CATACGGCAG 
GAAAACAAAG CTATTAAAGA AAAGCTGCTG TCCTTGCCCG GCTGTGAGAG GTTACTGGAA 
GCCATAGGCT TCGTACGCGC CCCCAGTTCC AACGCCTACA CCTTGCCCAC AGAGGTGTCA 
CTGCAGCAGG TGAAGAAGTA CAGAGATGCG CTGAGCGAGA GACGCACTGC CTGGCTTAAC 
GGCACAGTGT CAAAAAGTCC TCCTCAACAA AGCACCACCA GCACCACGCC CTTGTTCATC 
AAGCCTTCTG TGGAATATCG CCATAGAATC GCCTTCCCCC GTGTATTGCG CACCAATAAT 
AACTTTCTGC AGTCGCTAGA GCTTTATTCC GATGCAGTCA TGCAGTACGA GGATAACCTG 
CTGCTGGCTA CTGGCCGCAC TCTGATTCCT GTGGAAGAGC TCACCGAAAT GGCAAGCGAG 
AAACTGATAG ACATCCAAGA TCAAATTGCG TCGGGTGAGC GCCAGGAGAA GGAGCCATGT 
GTTCGCGACT TGCTGCTTGT GGAGCTGGTA AACTGGTTCA ACACGCAGTT CTTCCAGTGG 
GTAAACAATA TACCTTGCCG CGTGTGTGGG AGTGAAGAGA GCAGGCTGCG CCGCACTGAA 
CGGGAAGGTG ATATAAGGGT GGAAGTCACC GTTTGCTGCG GCCAGGAGAG CAAGTTCTAC 
CGCTATAACG ACATTTCCCA GCTCCTAGTT TCCCGTAAGG GCCGTTGCGG AGAATATGCC 
AATTGCTTCA CATTCCTCTG TCGCGCCCTC GACTACGACG CCCGTATTGT CCACTCGCAT 
TTTGATCACG TCTGGACCGA GGTGTACTCC GAGGCCCAGA TGCGTTGGCT CCATGTTGAC 
CCCTCCGAGA ACGTTATAGA TTCTCCGCTG ATGTATCAGC ATGGCTGGAA ACGTCACATC 
GACTACATTC TTGCATACTC TAGGGACGAC ATCCAAGATG TGACTTGGCG CTATACTAAT 
GACCACCAGA AGATTCTACA CCTACGAAAG CTGTGCGGGG AAAAAGAAAT GGTGCAAACG 
CTGGACGCGA TTCGAGCAAA GCGGCGGCAA AATTGCACCG CCGATCGAAA GTTATTCCTC 
AGCCAGCGCA ATATGTACGA GGTCATCGGC TTGACGCTAG AGCGTAAGCC GACAGAAAAC 
GAGCTGAAGG GTCGGAGTTC GGGAAGTCTT TCTTGGCGGC AGTCACGTGG AGAGCATACT 
TTTACCAATA TATTCGTTTT CAATCTAAGT GCAACCGAAC TACAAAAAAG GCAACTTAAT 
GTGCGTTACA GCTGTGCCAC CGATACATAC GAACGATATG CCAAAGAAGG AGAACACATC 
ACTATTTTGG ATAGCTATAA GACATGGCAA AAAGCGCAAT TTTCCTCAAA GAACATATTT 
CGGAAAGTGG AACGCGATTG GAAAATGGCA TATTTGGCTA GACTGGAGGA TACCGATTGC 
GGAGAAATTG CCTGGACGTT TGATTTCAGC AAGACCAATT TAAAGGTTAA GTCTTACAAC 
TTAGTTTTCG AGACAAAAAC TTTTGGCGAC GGAAAAATCA GCGTGACTGT CGATGCCACC 
GACGGAAGCG CTTCGGTTGA AAATGCCACT GGATTCAAAA TAGTGGCCAA ACTTACGGGT 
GGCAAGGGCG ATGTAGCATG GCAACACACA CAACTGTTCA GGCAGAGTCT TAATTCAAGA 
GACTATCCCT TCGATCTGCA AGTGCAACTA CATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Pngl cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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