Ngly1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ngly1
Entrez Gene ID	59007
Full Name	N-glycanase 1
Synonyms	1110002C09Rik,PNGase,Png1
General protein information	Preferred Names N-glycanase 1 Names peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase mPNGase peptide N-glycanase homolog peptide:N-glycanase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 14 Map Location: 14 A2\|14 7.08 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001362433.1	NP_001349362.1	peptide-N(4)-(N-acetyl-beta- glucosaminyl)asparagine amidase isoform 3
NM_001362432.1	NP_001349361.1	peptide-N(4)-(N-acetyl-beta- glucosaminyl)asparagine amidase isoform 2
NM_021504.3	NP_067479.2	peptide-N(4)-(N-acetyl-beta- glucosaminyl)asparagine amidase isoform 1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	NGLY1	NP_001179716.1
Rattus norvegicus (Norway rat)	Ngly1	NP_001014158.1
Neurospora crassa	NCU00651	XP_965791.1
Mus musculus (house mouse)	Ngly1	NP_067479.2
Danio rerio (zebrafish)	ngly1	NP_001018437.1
Homo sapiens (human)	NGLY1	NP_060767.2
	NGLY1	XP_001092796.1
Canis lupus familiaris (dog)	NGLY1	XP_003639867.1
Gallus gallus (chicken)	NGLY1	NP_001026159.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007390	XP_308442.4
Caenorhabditis elegans (roundworm)	png-1	NP_492913.1
Saccharomyces cerevisiae (baker's yeast)	PNG1	NP_015229.1
Kluyveromyces lactis	KLLA0E24223g	XP_455043.1
Magnaporthe oryzae (rice blast fungus)	MGG_03598	XP_003716307.1
Pan troglodytes (chimpanzee)	NGLY1	XP_003309721.1
Schizosaccharomyces pombe (fission yeast)	SPBC1709.14	NP_595447.1
Drosophila melanogaster (fruit fly)	Pngl	NP_724444.3
Eremothecium gossypii	AGOS_ABR193W	NP_983142.1
Xenopus tropicalis (tropical clawed frog)	ngly1	XP_002932449.1

Gene Ontology
Bibliography

Related articles in PubMed

Lethality of mice bearing a knockout of the Ngly1-gene is partially rescued by the additional deletion of the Engase gene.
Fujihira H, Masahara-Negishi Y, Tamura M, Huang C, Harada Y, Wakana S, Takakura D, Kawasaki N, Taniguchi N, Kondoh G, Yamashita T, Funakoshi Y, Suzuki T
PLoS genetics13(4)e1006696(2017 Apr)

Mitochondrial complex II regulates a distinct oxygen sensing mechanism in monocytes.
Sharma S, Wang J, Cortes Gomez E, Taggart RT, Baysal BE
Human molecular genetics26(7)1328-1339(2017 04)

Quantitative proteomic profiling of the extracellular matrix of pancreatic islets during the angiogenic switch and insulinoma progression.
Naba A, Clauser KR, Mani DR, Carr SA, Hynes RO
Scientific reports740495(2017 Jan)

Endo-?-N-acetylglucosaminidase forms N-GlcNAc protein aggregates during ER-associated degradation in Ngly1-defective cells.
Huang C, Harada Y, Hosomi A, Masahara-Negishi Y, Seino J, Fujihira H, Funakoshi Y, Suzuki T, Dohmae N, Suzuki T
Proceedings of the National Academy of Sciences of the United States of America112(5)1398-403(2015 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These observations strongly suggest that the Ngly1- or Ngly1/Engase-deficient mice could serve as a valuable animal model for studies related to the pathogenesis of the NGLY1-deficiency, and that cytoplasmic ENGase represents one of the potential therapeutic targets for this genetic disorder.
Title: Lethality of mice bearing a knockout of the Ngly1-gene is partially rescued by the additional deletion of the Engase gene.

study underscores the functional importance of Ngly1 in the ERAD process and provides a potential mechanism underlying the phenotypic consequences of a newly emerging genetic disorder caused by mutation of the human NGLY1 gene
Title: Endo-?-N-acetylglucosaminidase forms N-GlcNAc protein aggregates during ER-associated degradation in Ngly1-defective cells.

crystal structure of the N-terminal domain of PNGase in complex with this motif provides detailed insight into the interaction between p97 and its substrate-processing cofactors
Title: Studies on peptide:N-glycanase-p97 interaction suggest that p97 phosphorylation modulates endoplasmic reticulum-associated degradation.

enhances the activity of the mouse PNGase core domain, presumably by increasing the affinity of mouse PNGase for the glycan chains of misfolded glycoproteins
Title: Structural and biochemical studies of the C-terminal domain of mouse peptide-N-glycanase identify it as a mannose-binding module.

cytoplasmic protein mouse p97(mp97) participates in the formation of a ternary complex containing mouse autocrine motility factor receptor (mAMFR), mp97, and peptide N-glycanase
Title: The AAA ATPase p97 links peptide N-glycanase to the endoplasmic reticulum-associated E3 ligase autocrine motility factor receptor.

The following Ngly1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ngly1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu12423	NM_021504.3 Latest version!	Mus musculus N-glycanase 1 (Ngly1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OMu84294	NM_001362433.1 Latest version!	Mus musculus N-glycanase 1 (Ngly1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu84304	NM_001362432.1 Latest version!	Mus musculus N-glycanase 1 (Ngly1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ngly1 ( NM_021504.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_067479.2 Mus musculus N-glycanase 1 (Ngly1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu12423
Clone ID Related Accession (Same CDS sequence)	NM_021504.3
Accession Version	NM_021504.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-09
Organism	Mus musculus(house mouse)
Product	peptide-N(4)-(N-acetyl-beta- glucosaminyl)asparagine amidase isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK028248.1. On Apr 6, 2007 this sequence version replaced NM_021504.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK028248.1, SRR1660817.308996.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGTCGG CCACACTGGG CAGCTCGTCG AGCTCTGCGT CCCCGGCCGT GGCCGAGCTA 
TGCCAGAACA CCCCGGAGAC CTTTCTGGAG GCCTCCAAGC TGCTGCTCAC CTACGCCGAC 
AACATCCTGA GAAACCCCAG TGATGAAAAA TACAGATCCA TCCGTATTGG GAACACAGCG 
TTTTCTACTA GACTCTTGCC TGTCAGAGGA GCTGTTGAGT GTTTATTTGA AATGGGCTTT 
GAAGAGGGAG AAACGCATCT TATCTTTCCT AAAAAAGCTT CAGTGGAACA GCTACAGAAA 
ATCCGTGATC TGATTGCCAT AGAGAGAAGC AGCAGACTGG ACGGATCCAG TAAGAAGGTG 
CAATTCTCTC AGCACCCGGC TGCTGCCAAG CTTCCTCTGG AGCAGTCTGA AGATCCTGCT 
GGGTTAATAC GGCACTCAGG AAACCAAACA GGGCAGCTGC CAAGCCTACC CTCTGCTCCA 
ATGGTTGTTG GTGATTCAAC CATCTTAAAA GTTCTTCAGT CCAACATTCA GCATGTGCAG 
CTCTATGAAA ACCCTGTCCT CCAGGAGAAA GCATTGACTT GTATCCCAGT CAGTGAACTA 
AAAAGAAAAG CTCAAGAAAA GTTATTCAGA GCTAGAAAGT TAGATAAAGG TACTAATGTA 
AGTGATGAGG ACTTCCTTCT ACTGGAGCTC CTACACTGGT TTAAAGAGGA GTTTTTTCGC 
TGGGTGAATA ACATTGTGTG CAGCAAATGT GGTGGAGAGA CTAGATCTAG AGATGAGGCA 
TTGCTGCCCA ATGATGATGA ACTGAAGTGG GGTGCAAAGA ATGTGGAGAA TCATTACTGT 
GATGCCTGCC AGTTGAGCAA CCGCTTCCCA AGATATAACA ACCCCGAGAA ACTTTTGGAA 
ACAAGATGTG GACGCTGTGG TGAATGGGCC AATTGTTTTA CTCTGTGCTG CCGTGCATTA 
GGGTTTGAAG CTCGATATGT GTGGGATTAC ACAGACCATG TTTGGACAGA AGTCTATTCT 
CCATCTCAGC AGCGGTGGCT ACACTGTGAT GCATGTGAGG ATGTCTGTGA CAAACCCCTC 
CTTTATGAAA TAGGATGGGG CAAGAAGCTT TCCTATATAA TAGCATTCTC TAAAGATGAG 
GTAGTTGATG TCACCTGGCG ATACTCTTGT AAACATGATG AGGTGATGTC CAGGAGGACC 
AAGGTTAAAG AAGAGTTACT TCGAGAAACT ATTAATGGGC TTAATAAGCA GAGACAGCTA 
TCATTATCAG AAAGCAGAAG GAAAGAACTT CTCCAGAGGA TAATTGTGGA GCTTGTTGAA 
TTTATATCTC CTAAAACCCC AAGACCTGGA GAACTTGGTG GAAGGGTATC CGGGTCATTG 
GCTTGGAGAG TGGCCCGAGG TGAGACGGGT CTAGAGAGAA AGGAGATTCT GTTCATCCCT 
TCTGAAAATG AAAAGATTTC TAAGCAGTTT CACCTCCGTT ATGATATTGT GAGAGACCGT 
TACATTCGTG TCTCAGACAA CAACATAAAC ATTTCTGGAT GGGAGAATGG TGTGTGGAAA 
ATGGAATCCA TATTCAGAAA AGTCGAGAAA GACTGGAACA TGGTTTATTT GGCCCGAAAA 
GAAGGATCAT CTTTTGCTTA TATTTCCTGG AAGTTTGAAT GTGGGTCAGC TGGTCTAAAA 
GTAGATACTG TTTCCATCCG CACAAGTAGC CAAAGCTTTG AGTCTGGATC AGTGAGGTGG 
AAACTGCGAT CTGAGACAGC TCAAGTCAAC CTGCTGGGAG ATAAAAATCT ACGTTCCTAT 
AATGATTTTT CTGGTGCCAC AGAGGTTACA TTAGAAGCAG AGTTAAGCAG AGGAGATGGA 
GACGTTGCTT GGCAACATAC CCAACTGTTT AGACAAAGCT TAAATGACAG TGGAGAAAAT 
GGCTTGGAAA TAATTATAAC GTTCAATGAC CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067479.2
CDS	54..2009
Translation	MASATLGSSSSSASPAVAELCQNTPETFLEASKLLLTYADNILRNPSDEKYRSIRIGNTAFSTRLLPVRGAVECLFEMGFEEGETHLIFPKKASVEQLQKIRDLIAIERSSRLDGSSKKVQFSQHPAAAKLPLEQSEDPAGLIRHSGNQTGQLPSLPSAPMVVGDSTILKVLQSNIQHVQLYENPVLQEKALTCIPVSELKRKAQEKLFRARKLDKGTNVSDEDFLLLELLHWFKEEFFRWVNNIVCSKCGGETRSRDEALLPNDDELKWGAKNVENHYCDACQLSNRFPRYNNPEKLLETRCGRCGEWANCFTLCCRALGFEARYVWDYTDHVWTEVYSPSQQRWLHCDACEDVCDKPLLYEIGWGKKLSYIIAFSKDEVVDVTWRYSCKHDEVMSRRTKVKEELLRETINGLNKQRQLSLSESRRKELLQRIIVELVEFISPKTPRPGELGGRVSGSLAWRVARGETGLERKEILFIPSENEKISKQFHLRYDIVRDRYIRVSDNNINISGWENGVWKMESIFRKVEKDWNMVYLARKEGSSFAYISWKFECGSAGLKVDTVSIRTSSQSFESGSVRWKLRSETAQVNLLGDKNLRSYNDFSGATEVTLEAELSRGDGDVAWQHTQLFRQSLNDSGENGLEIIITFNDL

Target ORF information:

RefSeq Version	NM_021504.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus N-glycanase 1 (Ngly1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021504.3

ORF Insert Sequence:

ATGGCGTCGG CCACACTGGG CAGCTCGTCG AGCTCTGCGT CCCCGGCCGT GGCCGAGCTA 
TGCCAGAACA CCCCGGAGAC CTTTCTGGAG GCCTCCAAGC TGCTGCTCAC CTACGCCGAC 
AACATCCTGA GAAACCCCAG TGATGAAAAA TACAGATCCA TCCGTATTGG GAACACAGCG 
TTTTCTACTA GACTCTTGCC TGTCAGAGGA GCTGTTGAGT GTTTATTTGA AATGGGCTTT 
GAAGAGGGAG AAACGCATCT TATCTTTCCT AAAAAAGCTT CAGTGGAACA GCTACAGAAA 
ATCCGTGATC TGATTGCCAT AGAGAGAAGC AGCAGACTGG ACGGATCCAG TAAGAAGGTG 
CAATTCTCTC AGCACCCGGC TGCTGCCAAG CTTCCTCTGG AGCAGTCTGA AGATCCTGCT 
GGGTTAATAC GGCACTCAGG AAACCAAACA GGGCAGCTGC CAAGCCTACC CTCTGCTCCA 
ATGGTTGTTG GTGATTCAAC CATCTTAAAA GTTCTTCAGT CCAACATTCA GCATGTGCAG 
CTCTATGAAA ACCCTGTCCT CCAGGAGAAA GCATTGACTT GTATCCCAGT CAGTGAACTA 
AAAAGAAAAG CTCAAGAAAA GTTATTCAGA GCTAGAAAGT TAGATAAAGG TACTAATGTA 
AGTGATGAGG ACTTCCTTCT ACTGGAGCTC CTACACTGGT TTAAAGAGGA GTTTTTTCGC 
TGGGTGAATA ACATTGTGTG CAGCAAATGT GGTGGAGAGA CTAGATCTAG AGATGAGGCA 
TTGCTGCCCA ATGATGATGA ACTGAAGTGG GGTGCAAAGA ATGTGGAGAA TCATTACTGT 
GATGCCTGCC AGTTGAGCAA CCGCTTCCCA AGATATAACA ACCCCGAGAA ACTTTTGGAA 
ACAAGATGTG GACGCTGTGG TGAATGGGCC AATTGTTTTA CTCTGTGCTG CCGTGCATTA 
GGGTTTGAAG CTCGATATGT GTGGGATTAC ACAGACCATG TTTGGACAGA AGTCTATTCT 
CCATCTCAGC AGCGGTGGCT ACACTGTGAT GCATGTGAGG ATGTCTGTGA CAAACCCCTC 
CTTTATGAAA TAGGATGGGG CAAGAAGCTT TCCTATATAA TAGCATTCTC TAAAGATGAG 
GTAGTTGATG TCACCTGGCG ATACTCTTGT AAACATGATG AGGTGATGTC CAGGAGGACC 
AAGGTTAAAG AAGAGTTACT TCGAGAAACT ATTAATGGGC TTAATAAGCA GAGACAGCTA 
TCATTATCAG AAAGCAGAAG GAAAGAACTT CTCCAGAGGA TAATTGTGGA GCTTGTTGAA 
TTTATATCTC CTAAAACCCC AAGACCTGGA GAACTTGGTG GAAGGGTATC CGGGTCATTG 
GCTTGGAGAG TGGCCCGAGG TGAGACGGGT CTAGAGAGAA AGGAGATTCT GTTCATCCCT 
TCTGAAAATG AAAAGATTTC TAAGCAGTTT CACCTCCGTT ATGATATTGT GAGAGACCGT 
TACATTCGTG TCTCAGACAA CAACATAAAC ATTTCTGGAT GGGAGAATGG TGTGTGGAAA 
ATGGAATCCA TATTCAGAAA AGTCGAGAAA GACTGGAACA TGGTTTATTT GGCCCGAAAA 
GAAGGATCAT CTTTTGCTTA TATTTCCTGG AAGTTTGAAT GTGGGTCAGC TGGTCTAAAA 
GTAGATACTG TTTCCATCCG CACAAGTAGC CAAAGCTTTG AGTCTGGATC AGTGAGGTGG 
AAACTGCGAT CTGAGACAGC TCAAGTCAAC CTGCTGGGAG ATAAAAATCT ACGTTCCTAT 
AATGATTTTT CTGGTGCCAC AGAGGTTACA TTAGAAGCAG AGTTAAGCAG AGGAGATGGA 
GACGTTGCTT GGCAACATAC CCAACTGTTT AGACAAAGCT TAAATGACAG TGGAGAAAAT 
GGCTTGGAAA TAATTATAAC GTTCAATGAC CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ngly1 ( NM_001362433.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001349362.1 Mus musculus N-glycanase 1 (Ngly1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu84294
Clone ID Related Accession (Same CDS sequence)	NM_001362433.1
Accession Version	NM_001362433.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1791bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Mus musculus(house mouse)
Product	peptide-N(4)-(N-acetyl-beta- glucosaminyl)asparagine amidase isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK028248.1, AK169205.1, AC173482.1 and BC028961.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK169205.1, SRR1660819.59499.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164134 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGTCGG CCACACTGGG CAGCTCGTCG AGCTCTGCGT CCCCGGCCGT GGCCGAGCTA 
TGCCAGAACA CCCCGGAGAC CTTTCTGGAG GCCTCCAAGC TGCTGCTCAC CTACGCCGAC 
AACATCCTGA GAAACCCCAG TGATGAAAAA TACAGATCCA TCCGTATTGG GAACACAGCG 
TTTTCTACTA GACTCTTGCC TGTCAGAGGA GCTGTTGAGT GTTTATTTGA AATGGGCTTT 
GAAGAGGGAG AAACGCATCT TATCTTTCCT AAAAAAGCTT CAGTGGAACA GCTACAGAAA 
ATCCGTGATC TGATTGCCAT AGAGAGAAGC AGCAGACTGG ACGGATCCAG TAAGAAGGTG 
CAATTCTCTC AGCACCCGGC TGCTGCCAAG CTTCCTCTGG AGCAGTCTGA AGATCCTGCT 
GGGTTAATAC GGCACTCAGG AAACCAAACA GGGCAGCTGC CAAGCCTACC CTCTGCTCCA 
ATGGTTGTTG GTGATTCAAC CATCTTAAAA GTTCTTCAGT CCAACATTCA GCATGTGCAG 
CTCTATGAAA ACCCTGTCCT CCAGGAGAAA GCATTGACTT GTATCCCAGT CAGTGAACTA 
AAAAGAAAAG CTCAAGAAAA GTTATTCAGA GCTAGAAAGT TAGATAAAGG TACTAATGTA 
AGTGATGAGG ACTTCCTTCT ACTGGAGCTC CTACACTGGT TTAAAGAGGA GTTTTTTCGC 
TGGGTGAATA ACATTGTGTG CAGCAAATGT GGTGGAGAGA CTAGATCTAG AGATGAGGCA 
TTGCTGCCCA ATGATGATGA ACTGAAGTGG GGTGCAAAGA ATGTGGAGAA TCATTACTGT 
GATGCCTGCC AGTTGAGCAA CCGCTTCCCA AGATATAACA ACCCCGAGAA ACTTTTGGAA 
ACAAGATGTG GACGCTGTGG TGAATGGGCC AATTGTTTTA CTCTGTGCTG CCGTGCATTA 
GGGTTTGAAG CTCGATATGT GTGGGATTAC ACAGACCATG TTTGGACAGA AGTCTATTCT 
CCATCTCAGC AGCGGTGGCT ACACTGTGAT GCATGTGAGG ATGTCTGTGA CAAACCCCTC 
CTTTATGAAA TAGGATGGGG CAAGAAGCTT TCCTATATAA TAGCATTCTC TAAAGATGAG 
GTAGTTGATG TCACCTGGCG ATACTCTTGT AAACATGATG AGGTGATGTC CAGGAGGACC 
AAGGTTAAAG AAGAGTTACT TCGAGAAACT ATTAATGGGC TTAATAAGCA GAGAAAGGAG 
ATTCTGTTCA TCCCTTCTGA AAATGAAAAG ATTTCTAAGC AGTTTCACCT CCGTTATGAT 
ATTGTGAGAG ACCGTTACAT TCGTGTCTCA GACAACAACA TAAACATTTC TGGATGGGAG 
AATGGTGTGT GGAAAATGGA ATCCATATTC AGAAAAGTCG AGAAAGACTG GAACATGGTT 
TATTTGGCCC GAAAAGAAGG ATCATCTTTT GCTTATATTT CCTGGAAGTT TGAATGTGGG 
TCAGCTGGTC TAAAAGTAGA TACTGTTTCC ATCCGCACAA GTAGCCAAAG CTTTGAGTCT 
GGATCAGTGA GGTGGAAACT GCGATCTGAG ACAGCTCAAG TCAACCTGCT GGGAGATAAA 
AATCTACGTT CCTATAATGA TTTTTCTGGT GCCACAGAGG TTACATTAGA AGCAGAGTTA 
AGCAGAGGAG ATGGAGACGT TGCTTGGCAA CATACCCAAC TGTTTAGACA AAGCTTAAAT 
GACAGTGGAG AAAATGGCTT GGAAATAATT ATAACGTTCA ATGACCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001349362.1
CDS	54..1844
Translation	MASATLGSSSSSASPAVAELCQNTPETFLEASKLLLTYADNILRNPSDEKYRSIRIGNTAFSTRLLPVRGAVECLFEMGFEEGETHLIFPKKASVEQLQKIRDLIAIERSSRLDGSSKKVQFSQHPAAAKLPLEQSEDPAGLIRHSGNQTGQLPSLPSAPMVVGDSTILKVLQSNIQHVQLYENPVLQEKALTCIPVSELKRKAQEKLFRARKLDKGTNVSDEDFLLLELLHWFKEEFFRWVNNIVCSKCGGETRSRDEALLPNDDELKWGAKNVENHYCDACQLSNRFPRYNNPEKLLETRCGRCGEWANCFTLCCRALGFEARYVWDYTDHVWTEVYSPSQQRWLHCDACEDVCDKPLLYEIGWGKKLSYIIAFSKDEVVDVTWRYSCKHDEVMSRRTKVKEELLRETINGLNKQRKEILFIPSENEKISKQFHLRYDIVRDRYIRVSDNNINISGWENGVWKMESIFRKVEKDWNMVYLARKEGSSFAYISWKFECGSAGLKVDTVSIRTSSQSFESGSVRWKLRSETAQVNLLGDKNLRSYNDFSGATEVTLEAELSRGDGDVAWQHTQLFRQSLNDSGENGLEIIITFNDL

Target ORF information:

RefSeq Version	NM_001362433.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus N-glycanase 1 (Ngly1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001362433.1

ORF Insert Sequence:

ATGGCGTCGG CCACACTGGG CAGCTCGTCG AGCTCTGCGT CCCCGGCCGT GGCCGAGCTA 
TGCCAGAACA CCCCGGAGAC CTTTCTGGAG GCCTCCAAGC TGCTGCTCAC CTACGCCGAC 
AACATCCTGA GAAACCCCAG TGATGAAAAA TACAGATCCA TCCGTATTGG GAACACAGCG 
TTTTCTACTA GACTCTTGCC TGTCAGAGGA GCTGTTGAGT GTTTATTTGA AATGGGCTTT 
GAAGAGGGAG AAACGCATCT TATCTTTCCT AAAAAAGCTT CAGTGGAACA GCTACAGAAA 
ATCCGTGATC TGATTGCCAT AGAGAGAAGC AGCAGACTGG ACGGATCCAG TAAGAAGGTG 
CAATTCTCTC AGCACCCGGC TGCTGCCAAG CTTCCTCTGG AGCAGTCTGA AGATCCTGCT 
GGGTTAATAC GGCACTCAGG AAACCAAACA GGGCAGCTGC CAAGCCTACC CTCTGCTCCA 
ATGGTTGTTG GTGATTCAAC CATCTTAAAA GTTCTTCAGT CCAACATTCA GCATGTGCAG 
CTCTATGAAA ACCCTGTCCT CCAGGAGAAA GCATTGACTT GTATCCCAGT CAGTGAACTA 
AAAAGAAAAG CTCAAGAAAA GTTATTCAGA GCTAGAAAGT TAGATAAAGG TACTAATGTA 
AGTGATGAGG ACTTCCTTCT ACTGGAGCTC CTACACTGGT TTAAAGAGGA GTTTTTTCGC 
TGGGTGAATA ACATTGTGTG CAGCAAATGT GGTGGAGAGA CTAGATCTAG AGATGAGGCA 
TTGCTGCCCA ATGATGATGA ACTGAAGTGG GGTGCAAAGA ATGTGGAGAA TCATTACTGT 
GATGCCTGCC AGTTGAGCAA CCGCTTCCCA AGATATAACA ACCCCGAGAA ACTTTTGGAA 
ACAAGATGTG GACGCTGTGG TGAATGGGCC AATTGTTTTA CTCTGTGCTG CCGTGCATTA 
GGGTTTGAAG CTCGATATGT GTGGGATTAC ACAGACCATG TTTGGACAGA AGTCTATTCT 
CCATCTCAGC AGCGGTGGCT ACACTGTGAT GCATGTGAGG ATGTCTGTGA CAAACCCCTC 
CTTTATGAAA TAGGATGGGG CAAGAAGCTT TCCTATATAA TAGCATTCTC TAAAGATGAG 
GTAGTTGATG TCACCTGGCG ATACTCTTGT AAACATGATG AGGTGATGTC CAGGAGGACC 
AAGGTTAAAG AAGAGTTACT TCGAGAAACT ATTAATGGGC TTAATAAGCA GAGAAAGGAG 
ATTCTGTTCA TCCCTTCTGA AAATGAAAAG ATTTCTAAGC AGTTTCACCT CCGTTATGAT 
ATTGTGAGAG ACCGTTACAT TCGTGTCTCA GACAACAACA TAAACATTTC TGGATGGGAG 
AATGGTGTGT GGAAAATGGA ATCCATATTC AGAAAAGTCG AGAAAGACTG GAACATGGTT 
TATTTGGCCC GAAAAGAAGG ATCATCTTTT GCTTATATTT CCTGGAAGTT TGAATGTGGG 
TCAGCTGGTC TAAAAGTAGA TACTGTTTCC ATCCGCACAA GTAGCCAAAG CTTTGAGTCT 
GGATCAGTGA GGTGGAAACT GCGATCTGAG ACAGCTCAAG TCAACCTGCT GGGAGATAAA 
AATCTACGTT CCTATAATGA TTTTTCTGGT GCCACAGAGG TTACATTAGA AGCAGAGTTA 
AGCAGAGGAG ATGGAGACGT TGCTTGGCAA CATACCCAAC TGTTTAGACA AAGCTTAAAT 
GACAGTGGAG AAAATGGCTT GGAAATAATT ATAACGTTCA ATGACCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ngly1 ( NM_001362432.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001349361.1 Mus musculus N-glycanase 1 (Ngly1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu84304
Clone ID Related Accession (Same CDS sequence)	NM_001362432.1
Accession Version	NM_001362432.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1899bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-09
Organism	Mus musculus(house mouse)
Product	peptide-N(4)-(N-acetyl-beta- glucosaminyl)asparagine amidase isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK148931.1, AC173482.1 and BC028961.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK148931.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCAGAAC ACCCCGGAGA CCTTTCTGGA GGCCTCCAAG CTGCTGCTCA CCTACGCCGA 
CAACATCCTG AGGTAAACCC CAGTGATGAA AAATACAGAT CCATCCGTAT TGGGAACACA 
GCGTTTTCTA CTAGACTCTT GCCTGTCAGA GGAGCTGTTG AGTGTTTATT TGAAATGGGC 
TTTGAAGAGG GAGAAACGCA TCTTATCTTT CCTAAAAAAG CTTCAGTGGA ACAGCTACAG 
AAAATCCGTG ATCTGATTGC CATAGAGAGA AGCAGCAGAC TGGACGGATC CAGTAAGAAG 
GTGCAATTCT CTCAGCACCC GGCTGCTGCC AAGCTTCCTC TGGAGCAGTC TGAAGATCCT 
GCTGGGTTAA TACGGCACTC AGGAAACCAA ACAGGGCAGC TGCCAAGCCT ACCCTCTGCT 
CCAATGGTTG TTGGTGATTC AACCATCTTA AAAGTTCTTC AGTCCAACAT TCAGCATGTG 
CAGCTCTATG AAAACCCTGT CCTCCAGGAG AAAGCATTGA CTTGTATCCC AGTCAGTGAA 
CTAAAAAGAA AAGCTCAAGA AAAGTTATTC AGAGCTAGAA AGTTAGATAA AGGTACTAAT 
GTAAGTGATG AGGACTTCCT TCTACTGGAG CTCCTACACT GGTTTAAAGA GGAGTTTTTT 
CGCTGGGTGA ATAACATTGT GTGCAGCAAA TGTGGTGGAG AGACTAGATC TAGAGATGAG 
GCATTGCTGC CCAATGATGA TGAACTGAAG TGGGGTGCAA AGAATGTGGA GAATCATTAC 
TGTGATGCCT GCCAGTTGAG CAACCGCTTC CCAAGATATA ACAACCCCGA GAAACTTTTG 
GAAACAAGAT GTGGACGCTG TGGTGAATGG GCCAATTGTT TTACTCTGTG CTGCCGTGCA 
TTAGGGTTTG AAGCTCGATA TGTGTGGGAT TACACAGACC ATGTTTGGAC AGAAGTCTAT 
TCTCCATCTC AGCAGCGGTG GCTACACTGT GATGCATGTG AGGATGTCTG TGACAAACCC 
CTCCTTTATG AAATAGGATG GGGCAAGAAG CTTTCCTATA TAATAGCATT CTCTAAAGAT 
GAGGTAGTTG ATGTCACCTG GCGATACTCT TGTAAACATG ATGAGGTGAT GTCCAGGAGG 
ACCAAGGTTA AAGAAGAGTT ACTTCGAGAA ACTATTAATG GGCTTAATAA GCAGAGACAG 
CTATCATTAT CAGAAAGCAG AAGGAAAGAA CTTCTCCAGA GGATAATTGT GGAGCTTGTT 
GAATTTATAT CTCCTAAAAC CCCAAGACCT GGAGAACTTG GTGGAAGGGT ATCCGGGTCA 
TTGGCTTGGA GAGTGGCCCG AGGTGAGACG GGTCTAGAGA GAAAGGAGAT TCTGTTCATC 
CCTTCTGAAA ATGAAAAGAT TTCTAAGCAG TTTCACCTCC GTTATGATAT TGTGAGAGAC 
CGTTACATTC GTGTCTCAGA CAACAACATA AACATTTCTG GATGGGAGAA TGGTGTGTGG 
AAAATGGAAT CCATATTCAG AAAAGTCGAG AAAGACTGGA ACATGGTTTA TTTGGCCCGA 
AAAGAAGGAT CATCTTTTGC TTATATTTCC TGGAAGTTTG AATGTGGGTC AGCTGGTCTA 
AAAGTAGATA CTGTTTCCAT CCGCACAAGT AGCCAAAGCT TTGAGTCTGG ATCAGTGAGG 
TGGAAACTGC GATCTGAGAC AGCTCAAGTC AACCTGCTGG GAGATAAAAA TCTACGTTCC 
TATAATGATT TTTCTGGTGC CACAGAGGTT ACATTAGAAG CAGAGTTAAG CAGAGGAGAT 
GGAGACGTTG CTTGGCAACA TACCCAACTG TTTAGACAAA GCTTAAATGA CAGTGGAGAA 
AATGGCTTGG AAATAATTAT AACGTTCAAT GACCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001349361.1
CDS	113..2011
Translation	MPEHPGDLSGGLQAAAHLRRQHPEVNPSDEKYRSIRIGNTAFSTRLLPVRGAVECLFEMGFEEGETHLIFPKKASVEQLQKIRDLIAIERSSRLDGSSKKVQFSQHPAAAKLPLEQSEDPAGLIRHSGNQTGQLPSLPSAPMVVGDSTILKVLQSNIQHVQLYENPVLQEKALTCIPVSELKRKAQEKLFRARKLDKGTNVSDEDFLLLELLHWFKEEFFRWVNNIVCSKCGGETRSRDEALLPNDDELKWGAKNVENHYCDACQLSNRFPRYNNPEKLLETRCGRCGEWANCFTLCCRALGFEARYVWDYTDHVWTEVYSPSQQRWLHCDACEDVCDKPLLYEIGWGKKLSYIIAFSKDEVVDVTWRYSCKHDEVMSRRTKVKEELLRETINGLNKQRQLSLSESRRKELLQRIIVELVEFISPKTPRPGELGGRVSGSLAWRVARGETGLERKEILFIPSENEKISKQFHLRYDIVRDRYIRVSDNNINISGWENGVWKMESIFRKVEKDWNMVYLARKEGSSFAYISWKFECGSAGLKVDTVSIRTSSQSFESGSVRWKLRSETAQVNLLGDKNLRSYNDFSGATEVTLEAELSRGDGDVAWQHTQLFRQSLNDSGENGLEIIITFNDL

Target ORF information:

RefSeq Version	NM_001362432.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus N-glycanase 1 (Ngly1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001362432.1

ORF Insert Sequence:

ATGCCAGAAC ACCCCGGAGA CCTTTCTGGA GGCCTCCAAG CTGCTGCTCA CCTACGCCGA 
CAACATCCTG AGGTAAACCC CAGTGATGAA AAATACAGAT CCATCCGTAT TGGGAACACA 
GCGTTTTCTA CTAGACTCTT GCCTGTCAGA GGAGCTGTTG AGTGTTTATT TGAAATGGGC 
TTTGAAGAGG GAGAAACGCA TCTTATCTTT CCTAAAAAAG CTTCAGTGGA ACAGCTACAG 
AAAATCCGTG ATCTGATTGC CATAGAGAGA AGCAGCAGAC TGGACGGATC CAGTAAGAAG 
GTGCAATTCT CTCAGCACCC GGCTGCTGCC AAGCTTCCTC TGGAGCAGTC TGAAGATCCT 
GCTGGGTTAA TACGGCACTC AGGAAACCAA ACAGGGCAGC TGCCAAGCCT ACCCTCTGCT 
CCAATGGTTG TTGGTGATTC AACCATCTTA AAAGTTCTTC AGTCCAACAT TCAGCATGTG 
CAGCTCTATG AAAACCCTGT CCTCCAGGAG AAAGCATTGA CTTGTATCCC AGTCAGTGAA 
CTAAAAAGAA AAGCTCAAGA AAAGTTATTC AGAGCTAGAA AGTTAGATAA AGGTACTAAT 
GTAAGTGATG AGGACTTCCT TCTACTGGAG CTCCTACACT GGTTTAAAGA GGAGTTTTTT 
CGCTGGGTGA ATAACATTGT GTGCAGCAAA TGTGGTGGAG AGACTAGATC TAGAGATGAG 
GCATTGCTGC CCAATGATGA TGAACTGAAG TGGGGTGCAA AGAATGTGGA GAATCATTAC 
TGTGATGCCT GCCAGTTGAG CAACCGCTTC CCAAGATATA ACAACCCCGA GAAACTTTTG 
GAAACAAGAT GTGGACGCTG TGGTGAATGG GCCAATTGTT TTACTCTGTG CTGCCGTGCA 
TTAGGGTTTG AAGCTCGATA TGTGTGGGAT TACACAGACC ATGTTTGGAC AGAAGTCTAT 
TCTCCATCTC AGCAGCGGTG GCTACACTGT GATGCATGTG AGGATGTCTG TGACAAACCC 
CTCCTTTATG AAATAGGATG GGGCAAGAAG CTTTCCTATA TAATAGCATT CTCTAAAGAT 
GAGGTAGTTG ATGTCACCTG GCGATACTCT TGTAAACATG ATGAGGTGAT GTCCAGGAGG 
ACCAAGGTTA AAGAAGAGTT ACTTCGAGAA ACTATTAATG GGCTTAATAA GCAGAGACAG 
CTATCATTAT CAGAAAGCAG AAGGAAAGAA CTTCTCCAGA GGATAATTGT GGAGCTTGTT 
GAATTTATAT CTCCTAAAAC CCCAAGACCT GGAGAACTTG GTGGAAGGGT ATCCGGGTCA 
TTGGCTTGGA GAGTGGCCCG AGGTGAGACG GGTCTAGAGA GAAAGGAGAT TCTGTTCATC 
CCTTCTGAAA ATGAAAAGAT TTCTAAGCAG TTTCACCTCC GTTATGATAT TGTGAGAGAC 
CGTTACATTC GTGTCTCAGA CAACAACATA AACATTTCTG GATGGGAGAA TGGTGTGTGG 
AAAATGGAAT CCATATTCAG AAAAGTCGAG AAAGACTGGA ACATGGTTTA TTTGGCCCGA 
AAAGAAGGAT CATCTTTTGC TTATATTTCC TGGAAGTTTG AATGTGGGTC AGCTGGTCTA 
AAAGTAGATA CTGTTTCCAT CCGCACAAGT AGCCAAAGCT TTGAGTCTGG ATCAGTGAGG 
TGGAAACTGC GATCTGAGAC AGCTCAAGTC AACCTGCTGG GAGATAAAAA TCTACGTTCC 
TATAATGATT TTTCTGGTGC CACAGAGGTT ACATTAGAAG CAGAGTTAAG CAGAGGAGAT 
GGAGACGTTG CTTGGCAACA TACCCAACTG TTTAGACAAA GCTTAAATGA CAGTGGAGAA 
AATGGCTTGG AAATAATTAT AACGTTCAAT GACCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Lethality of mice bearing a knockout of the Ngly1-gene is partially rescued by the additional deletion of the Engase gene.
PLoS genetics13(4)e1006696(2017 Apr)
Fujihira H,Masahara-Negishi Y,Tamura M,Huang C,Harada Y,Wakana S,Takakura D,Kawasaki N,Taniguchi N,Kondoh G,Yamashita T,Funakoshi Y,Suzuki T

Mitochondrial complex II regulates a distinct oxygen sensing mechanism in monocytes.
Human molecular genetics26(7)1328-1339(2017 04)
Sharma S,Wang J,Cortes Gomez E,Taggart RT,Baysal BE

Quantitative proteomic profiling of the extracellular matrix of pancreatic islets during the angiogenic switch and insulinoma progression.
Scientific reports740495(2017 Jan)
Naba A,Clauser KR,Mani DR,Carr SA,Hynes RO

Endo-?-N-acetylglucosaminidase forms N-GlcNAc protein aggregates during ER-associated degradation in Ngly1-defective cells.
Proceedings of the National Academy of Sciences of the United States of America112(5)1398-403(2015 Feb)
Huang C,Harada Y,Hosomi A,Masahara-Negishi Y,Seino J,Fujihira H,Funakoshi Y,Suzuki T,Dohmae N,Suzuki T

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Ngly1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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