NEK8 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	NEK8
Entrez Gene ID	532332
Full Name	NIMA related kinase 8
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024980245.1	XP_024836013.1	serine/threonine-protein kinase Nek8 isoform X1
NM_001192723.1	NP_001179652.1	serine/threonine-protein kinase Nek8

Pathways from BioSystems

Homologies

	NEK8	XP_001106986.2
Drosophila melanogaster (fruit fly)	niki	NP_651293.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006872	XP_308885.4
Canis lupus familiaris (dog)	NEK8	XP_548291.3
Pan troglodytes (chimpanzee)	NEK8	XP_511801.3
Mus musculus (house mouse)	Nek8	NP_543125.1
Homo sapiens (human)	NEK8	NP_835464.1
Rattus norvegicus (Norway rat)	Nek8	NP_001099274.1
Danio rerio (zebrafish)	nek8	NP_620776.1
Bos taurus (cattle)	NEK8	NP_001179652.1
Gallus gallus (chicken)	NEK8	XP_415822.3
Caenorhabditis elegans (roundworm)	nekl-1	NP_490968.2
Xenopus tropicalis (tropical clawed frog)	nek8	NP_001006906.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NEK8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NEK8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa38221	NM_001192723.1 Latest version!	Bos taurus NIMA related kinase 8 (NEK8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa226432	XM_024980245.1 Latest version!	Bos taurus NIMA related kinase 8 (NEK8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NEK8 ( NM_001192723.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001179652.1 Bos taurus NIMA related kinase 8 (NEK8), mRNA.

Price & Availability↑

CloneID	OBa38221
Clone ID Related Accession (Same CDS sequence)	NM_001192723.1
Accession Version	NM_001192723.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-01
Organism	Bos taurus(cattle)
Product	serine/threonine-protein kinase Nek8
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000019.1. On Jul 17, 2010 this sequence version replaced XM_610844.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END##

ATGGAGAAGT ACGAGCGGAT CCGAGTGGTG GGCAGAGGTG CCTTCGGGAT TGTTCATCTG 
TGCTTGCGCA AAGCTGACCA GAAGCTGGTG ATCATCAAGC AGATCCCTGT AGAGCAGATG 
ACTAAGGAAG AGCGGCAGGC AGCCCAGAAC GAGTGCCAGG TGCTCAAGCT GCTCAACCAT 
CCCAACGTCA TTGAGTACTA CGAGAACTTC CTGGAGGACA AGGCTCTCAT GATCGCCATG 
GAGTACGCAC CAGGCGGCAC CCTGGCCGAG TTCATCCAGA AACGCTGTAA CTCCCTGCTG 
GAGGAGGAGA CCATCCTGCA CTTCTTCGTG CAGATCCTGC TGGCGCTGCA TCACGTCCAC 
AGCCACCTCA TCCTGCACCG GGATCTCAAA ACCCAAAACA TCCTTCTCGA TAAACACCGC 
ATGGTCGTCA AGATCGGCGA CTTTGGCATC TCCAAGATCC TTAGCAGCAA GAGCAAGGCC 
TACACGGTGG TGGGTACTCC CTGCTACATT TCCCCAGAAC TGTGTGAGGG CAAGCCCTAC 
AACCAGAAGA GTGACATCTG GGCCCTGGGC TGTGTCCTCT ATGAGTTGGC CAGCCTCAAG 
AGGGCCTTCG AGGCTGCGAA CCTGCCAGCG CTGGTACTAA AGATCATGAG CGGTACCTTC 
GCGCCCATCT CTGACCGATA CAGCCCGGAG CTGCGCCAGC TGGTTCTGAG TCTGCTCAGC 
CTGGAACCCG CCCAGCGGCC GCCGCTCAGC CACATCATGG CGCAGCCCCT CTGCATCCGG 
GCGCTCCTCA ACCTGCACAC GGACCTGGGC AGTGTCCGCA TGCGGAGGGC AGAGAAGCCC 
TTGGCCACTG GGCCACCCAT GACCCCGGGC AGTACAGGAA GCAGGACCAC CAGCGCCCGG 
TGCAGAGGCG TCCCCCGGGG ACCTGCACGG CCGGCCATCC CGCCACCGCT GTCGTCGGTG 
TACACGTGGG GCGGCGGGCT CAGCGCGCCA CTCCGGCTGC CCATGCTCAA CACGGAGGTG 
GTCCAGGTGG CAGCCGGGCG CACGCAGAAG GCCGGCGTCA CTCGCTCCGG GCGCCTCATC 
CTTTGGGAGG CCCCGCCCCT TGGCGCGGGA GGTGGCGCCC TCCTGCCCGG GGCGGTGGAG 
CAGCAGCAGC CCCAGTTCGT CTCCCGTTTC CTGGAGGGTC AGTCGGGTGT GACTATCAAG 
CACGTGGCCT GCGGGGACCT CTTCACCGCC TGCCTGACTG ACCGAGGCAT CATCATGACC 
TTTGGCAGTG GTAGCAACGG GTGCCTAGGC CATGGCGGCC TCAATGACAT CAGCCAGCCC 
ACCATTGTGG AGGCCCTGCT GGGCTATGAG ATGGTGCAGG TGGCTTGTGG GGCCTCTCAT 
GTGCTGGCCT TGTCCACTGA GCGAGAACTA TTTGCTTGGG GCCGCGGAGA TGGTGGTCGG 
CTGGGACTAG GCACCAGGGA GTCCCACAGC TGCCCCCAGC AGGTACCCAT GCCCCCAGGA 
CAGGAAGCCC AGAGGGTTAT ATGTGGCATT GACTCCTCTG TGATCCTCAC AGTGCCTGGC 
CAAGCCCTGG CCTGTGGGAG CAACAGGTTC AACAAGCTGG GCCTGGACCA CCTCTCCCTA 
GGGGAGGAGC CTGCCCTACA CCAGCAAGTG GAGGAGGCCC TGAACTTCAC TCCATTAGGT 
TCTGCACCCC TGGACCAGGA GCCCCTGCTG AGTGTGGATT TGGGCACTGC TCATTCAGCT 
GCTGTAACTG CCTCTGGTGA CTGCTACACT TTCGGCAGCA ATCAGCATGG GCAGTTGGGT 
ACCAATGCTC GCCGGGTCAG CCGGGCACCC TGTCAAGTCC AGGGCCTCCA GGGCATCAAG 
ATAGCGATGG TGGCCTGTGG GGATGCCTTC ACTGTTGCCA TTGGGGCAGA AGGTGAAGTG 
TACTCCTGGG GCAAAGGGGC CCGAGGTCGC CTGGGAAGGA GAGATGAGGA CGCCGCACTC 
CCTAGGCCAG TGCAGCTGGA TGAGACCCAG CCCTACACAG TGACCTCTGT GTCCTGTTGC 
CATGGCAACA CTCTCCTGGC TATCCGCCCT GTCACGGATG AGCCAGTCCC CCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001179652.1
CDS	45..2141
Translation	MEKYERIRVVGRGAFGIVHLCLRKADQKLVIIKQIPVEQMTKEERQAAQNECQVLKLLNHPNVIEYYENFLEDKALMIAMEYAPGGTLAEFIQKRCNSLLEEETILHFFVQILLALHHVHSHLILHRDLKTQNILLDKHRMVVKIGDFGISKILSSKSKAYTVVGTPCYISPELCEGKPYNQKSDIWALGCVLYELASLKRAFEAANLPALVLKIMSGTFAPISDRYSPELRQLVLSLLSLEPAQRPPLSHIMAQPLCIRALLNLHTDLGSVRMRRAEKPLATGPPMTPGSTGSRTTSARCRGVPRGPARPAIPPPLSSVYTWGGGLSAPLRLPMLNTEVVQVAAGRTQKAGVTRSGRLILWEAPPLGAGGGALLPGAVEQQQPQFVSRFLEGQSGVTIKHVACGDLFTACLTDRGIIMTFGSGSNGCLGHGGLNDISQPTIVEALLGYEMVQVACGASHVLALSTERELFAWGRGDGGRLGLGTRESHSCPQQVPMPPGQEAQRVICGIDSSVILTVPGQALACGSNRFNKLGLDHLSLGEEPALHQQVEEALNFTPLGSAPLDQEPLLSVDLGTAHSAAVTASGDCYTFGSNQHGQLGTNARRVSRAPCQVQGLQGIKIAMVACGDAFTVAIGAEGEVYSWGKGARGRLGRRDEDAALPRPVQLDETQPYTVTSVSCCHGNTLLAIRPVTDEPVPP

Target ORF information:

RefSeq Version	NM_001192723.1
Organism	Bos taurus(cattle)
Definition	Bos taurus NIMA related kinase 8 (NEK8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001192723.1

ORF Insert Sequence:

ATGGAGAAGT ACGAGCGGAT CCGAGTGGTG GGCAGAGGTG CCTTCGGGAT TGTTCATCTG 
TGCTTGCGCA AAGCTGACCA GAAGCTGGTG ATCATCAAGC AGATCCCTGT AGAGCAGATG 
ACTAAGGAAG AGCGGCAGGC AGCCCAGAAC GAGTGCCAGG TGCTCAAGCT GCTCAACCAT 
CCCAACGTCA TTGAGTACTA CGAGAACTTC CTGGAGGACA AGGCTCTCAT GATCGCCATG 
GAGTACGCAC CAGGCGGCAC CCTGGCCGAG TTCATCCAGA AACGCTGTAA CTCCCTGCTG 
GAGGAGGAGA CCATCCTGCA CTTCTTCGTG CAGATCCTGC TGGCGCTGCA TCACGTCCAC 
AGCCACCTCA TCCTGCACCG GGATCTCAAA ACCCAAAACA TCCTTCTCGA TAAACACCGC 
ATGGTCGTCA AGATCGGCGA CTTTGGCATC TCCAAGATCC TTAGCAGCAA GAGCAAGGCC 
TACACGGTGG TGGGTACTCC CTGCTACATT TCCCCAGAAC TGTGTGAGGG CAAGCCCTAC 
AACCAGAAGA GTGACATCTG GGCCCTGGGC TGTGTCCTCT ATGAGTTGGC CAGCCTCAAG 
AGGGCCTTCG AGGCTGCGAA CCTGCCAGCG CTGGTACTAA AGATCATGAG CGGTACCTTC 
GCGCCCATCT CTGACCGATA CAGCCCGGAG CTGCGCCAGC TGGTTCTGAG TCTGCTCAGC 
CTGGAACCCG CCCAGCGGCC GCCGCTCAGC CACATCATGG CGCAGCCCCT CTGCATCCGG 
GCGCTCCTCA ACCTGCACAC GGACCTGGGC AGTGTCCGCA TGCGGAGGGC AGAGAAGCCC 
TTGGCCACTG GGCCACCCAT GACCCCGGGC AGTACAGGAA GCAGGACCAC CAGCGCCCGG 
TGCAGAGGCG TCCCCCGGGG ACCTGCACGG CCGGCCATCC CGCCACCGCT GTCGTCGGTG 
TACACGTGGG GCGGCGGGCT CAGCGCGCCA CTCCGGCTGC CCATGCTCAA CACGGAGGTG 
GTCCAGGTGG CAGCCGGGCG CACGCAGAAG GCCGGCGTCA CTCGCTCCGG GCGCCTCATC 
CTTTGGGAGG CCCCGCCCCT TGGCGCGGGA GGTGGCGCCC TCCTGCCCGG GGCGGTGGAG 
CAGCAGCAGC CCCAGTTCGT CTCCCGTTTC CTGGAGGGTC AGTCGGGTGT GACTATCAAG 
CACGTGGCCT GCGGGGACCT CTTCACCGCC TGCCTGACTG ACCGAGGCAT CATCATGACC 
TTTGGCAGTG GTAGCAACGG GTGCCTAGGC CATGGCGGCC TCAATGACAT CAGCCAGCCC 
ACCATTGTGG AGGCCCTGCT GGGCTATGAG ATGGTGCAGG TGGCTTGTGG GGCCTCTCAT 
GTGCTGGCCT TGTCCACTGA GCGAGAACTA TTTGCTTGGG GCCGCGGAGA TGGTGGTCGG 
CTGGGACTAG GCACCAGGGA GTCCCACAGC TGCCCCCAGC AGGTACCCAT GCCCCCAGGA 
CAGGAAGCCC AGAGGGTTAT ATGTGGCATT GACTCCTCTG TGATCCTCAC AGTGCCTGGC 
CAAGCCCTGG CCTGTGGGAG CAACAGGTTC AACAAGCTGG GCCTGGACCA CCTCTCCCTA 
GGGGAGGAGC CTGCCCTACA CCAGCAAGTG GAGGAGGCCC TGAACTTCAC TCCATTAGGT 
TCTGCACCCC TGGACCAGGA GCCCCTGCTG AGTGTGGATT TGGGCACTGC TCATTCAGCT 
GCTGTAACTG CCTCTGGTGA CTGCTACACT TTCGGCAGCA ATCAGCATGG GCAGTTGGGT 
ACCAATGCTC GCCGGGTCAG CCGGGCACCC TGTCAAGTCC AGGGCCTCCA GGGCATCAAG 
ATAGCGATGG TGGCCTGTGG GGATGCCTTC ACTGTTGCCA TTGGGGCAGA AGGTGAAGTG 
TACTCCTGGG GCAAAGGGGC CCGAGGTCGC CTGGGAAGGA GAGATGAGGA CGCCGCACTC 
CCTAGGCCAG TGCAGCTGGA TGAGACCCAG CCCTACACAG TGACCTCTGT GTCCTGTTGC 
CATGGCAACA CTCTCCTGGC TATCCGCCCT GTCACGGATG AGCCAGTCCC CCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK8 ( XM_024980245.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024836013.1 Bos taurus NIMA related kinase 8 (NEK8), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa226432
Clone ID Related Accession (Same CDS sequence)	XM_024980245.1
Accession Version	XM_024980245.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1938bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	serine/threonine-protein kinase Nek8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGT ACGAGCGGAT CCGAGTGGTG GGCAGAGGTG CCTTCGGGAT TGTTCATCTG 
TGCTTGCGCA AAGCTGACCA GAAGCTGGTG ATCATCAAGC AGATCCCTGT AGAGCAGATG 
ACTAAGGAAG AGCGGCAGGC AGCCCAGAAC GAGTGCCAGG TGCTCAAGCT GCTCAACCAT 
CCCAACGTCA TTGAGTACTA CGAGAACTTC CTGGAGGACA AGGCTCTCAT GATCGCCATG 
GAGTACGCAC CAGGCGGCAC CCTGGCCGAG TTCATCCAGA AACGCTGTAA CTCCCTGCTG 
GAGGAGGAGA CCATCCTGCA CTTCTTCGTG CAGATCCTGC TGGCGCTGCA TCACGTCCAC 
AGCCACCTCA TCCTGCACCG GGATCTCAAA ACCCAAAACA TCCTTCTCGA TAAACACCGC 
ATGGTCGTCA AGATCGGCGA CTTTGGCATC TCCAAGATCC TTAGCAGCAA GAGCAAGGCC 
TACACGGTGG TGGGTACTCC CTGCTACATT TCCCCAGAAC TGTGTGAGGG CAAGCCCTAC 
AACCAGAAGA GTGACATCTG GGCCCTGGGC TGTGTCCTCT ATGAGTTGGC CAGCCTCAAG 
AGGGCCTTCG AGGCTGCGAA CCTGCCAGCG CTGGTACTAA AGATCATGAG CGGTACCTTC 
GCGCCCATCT CTGACCGATA CAGCCCGGAG CTGCGCCAGC TGGTTCTGAG TCTGCTCAGC 
CTGGAACCCG CCCAGCGGCC GCCGCTCAGC CACATCATGG CGCAGCCCCT CTGCATCCGG 
GCGCTCCTCA ACCTGCACAC GGACCTGGGC AGTGTCCGCA TGCGGAGGGC AGAGAAGCCC 
TTGGCCACTG GGCCACCCAT GACCCCGGGC AGTACAGGAA GCAGGACCAC CAGCGCCCGG 
TGCAGAGGCG TCCCCCGGGG ACCTGCACGG CCGGCCATCC CGCCACCGCT GTCGTCGGTG 
TACACGTGGG GCGGCGGGCT CAGCGCGCCA CTCCGGCTGC CCATGCTCAA CACGGAGGTG 
GTCCAGGTGG CAGCCGGGCG CACGCAGAAG GCCGGCGTCA CTCGCTCCGG GCGCCTCATC 
CTTTGGGAGG CCCCGCCCCT TGGCGCGGGA GGTGGCGCCC TCCTGCCCGG GGCGGTGGAG 
CAGCAGCAGC CCCAGTTCGT CTCCCGTTTC CTGGAGGGTC AGTCGGGTGT GACTATCAAG 
CACGTGGCCT GCGGGGACCT CTTCACCGCC TGCCTGACTG ACCGAGGCAT CATCATGACC 
TTTGGCAGTG GTAGCAACGG GTGCCTAGGC CATGGCGGCC TCAATGACAT CAGCCAGCCC 
ACCATTGTGG AGGCCCTGCT GGGCTATGAG ATGGTGCAGG TGGCTTGTGG GGCCTCTCAT 
GTGCTGGCCT TGTCCACTGA GCGAGAACTA TTTGCTTGGG GCCGCGGAGA TGGTGGTCGG 
CTGGGACTAG GCACCAGGGA GTCCCACAGC TGCCCCCAGC AGGTACCCAT GCCCCCAGGA 
CAGGAAGCCC AGAGGGTTAT ATGTGGCATT GACTCCTCTG TGATCCTCAC AGTGCCTGGC 
CAAGCCCTGG CCTGTGGGAG CAACAGGTTC AACAAGCTGG GCCTGGACCA CCTCTCCCTA 
GGGGAGGAGC CTGCCCTACA CCAGCAAGTG GAGGAGGCCC TGAACTTCAC TCCATTAGGT 
TCTGCACCCC TGGACCAGGA GCCCCTGCTG AGTGTGGATT TGGGCACTGC TCATTCAGCT 
GCTGTAACTG AAGGTGAAGT GTACTCCTGG GGCAAAGGGG CCCGAGGTCG CCTGGGAAGG 
AGAGATGAGG ACGCCGCACT CCCTAGGCCA GTGCAGCTGG ATGAGACCCA GCCCTACACA 
GTGACCTCTG TGTCCTGTTG CCATGGCAAC ACTCTCCTGG CTATCCGCCC TGTCACGGAT 
GAGCCAGTCC CCCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024836013.1
CDS	77..2014
Translation	MEKYERIRVVGRGAFGIVHLCLRKADQKLVIIKQIPVEQMTKEERQAAQNECQVLKLLNHPNVIEYYENFLEDKALMIAMEYAPGGTLAEFIQKRCNSLLEEETILHFFVQILLALHHVHSHLILHRDLKTQNILLDKHRMVVKIGDFGISKILSSKSKAYTVVGTPCYISPELCEGKPYNQKSDIWALGCVLYELASLKRAFEAANLPALVLKIMSGTFAPISDRYSPELRQLVLSLLSLEPAQRPPLSHIMAQPLCIRALLNLHTDLGSVRMRRAEKPLATGPPMTPGSTGSRTTSARCRGVPRGPARPAIPPPLSSVYTWGGGLSAPLRLPMLNTEVVQVAAGRTQKAGVTRSGRLILWEAPPLGAGGGALLPGAVEQQQPQFVSRFLEGQSGVTIKHVACGDLFTACLTDRGIIMTFGSGSNGCLGHGGLNDISQPTIVEALLGYEMVQVACGASHVLALSTERELFAWGRGDGGRLGLGTRESHSCPQQVPMPPGQEAQRVICGIDSSVILTVPGQALACGSNRFNKLGLDHLSLGEEPALHQQVEEALNFTPLGSAPLDQEPLLSVDLGTAHSAAVTEGEVYSWGKGARGRLGRRDEDAALPRPVQLDETQPYTVTSVSCCHGNTLLAIRPVTDEPVPP

Target ORF information:

RefSeq Version	XM_024980245.1
Organism	Bos taurus(cattle)
Definition	Bos taurus NIMA related kinase 8 (NEK8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024980245.1

ORF Insert Sequence:

ATGGAGAAGT ACGAGCGGAT CCGAGTGGTG GGCAGAGGTG CCTTCGGGAT TGTTCATCTG 
TGCTTGCGCA AAGCTGACCA GAAGCTGGTG ATCATCAAGC AGATCCCTGT AGAGCAGATG 
ACTAAGGAAG AGCGGCAGGC AGCCCAGAAC GAGTGCCAGG TGCTCAAGCT GCTCAACCAT 
CCCAACGTCA TTGAGTACTA CGAGAACTTC CTGGAGGACA AGGCTCTCAT GATCGCCATG 
GAGTACGCAC CAGGCGGCAC CCTGGCCGAG TTCATCCAGA AACGCTGTAA CTCCCTGCTG 
GAGGAGGAGA CCATCCTGCA CTTCTTCGTG CAGATCCTGC TGGCGCTGCA TCACGTCCAC 
AGCCACCTCA TCCTGCACCG GGATCTCAAA ACCCAAAACA TCCTTCTCGA TAAACACCGC 
ATGGTCGTCA AGATCGGCGA CTTTGGCATC TCCAAGATCC TTAGCAGCAA GAGCAAGGCC 
TACACGGTGG TGGGTACTCC CTGCTACATT TCCCCAGAAC TGTGTGAGGG CAAGCCCTAC 
AACCAGAAGA GTGACATCTG GGCCCTGGGC TGTGTCCTCT ATGAGTTGGC CAGCCTCAAG 
AGGGCCTTCG AGGCTGCGAA CCTGCCAGCG CTGGTACTAA AGATCATGAG CGGTACCTTC 
GCGCCCATCT CTGACCGATA CAGCCCGGAG CTGCGCCAGC TGGTTCTGAG TCTGCTCAGC 
CTGGAACCCG CCCAGCGGCC GCCGCTCAGC CACATCATGG CGCAGCCCCT CTGCATCCGG 
GCGCTCCTCA ACCTGCACAC GGACCTGGGC AGTGTCCGCA TGCGGAGGGC AGAGAAGCCC 
TTGGCCACTG GGCCACCCAT GACCCCGGGC AGTACAGGAA GCAGGACCAC CAGCGCCCGG 
TGCAGAGGCG TCCCCCGGGG ACCTGCACGG CCGGCCATCC CGCCACCGCT GTCGTCGGTG 
TACACGTGGG GCGGCGGGCT CAGCGCGCCA CTCCGGCTGC CCATGCTCAA CACGGAGGTG 
GTCCAGGTGG CAGCCGGGCG CACGCAGAAG GCCGGCGTCA CTCGCTCCGG GCGCCTCATC 
CTTTGGGAGG CCCCGCCCCT TGGCGCGGGA GGTGGCGCCC TCCTGCCCGG GGCGGTGGAG 
CAGCAGCAGC CCCAGTTCGT CTCCCGTTTC CTGGAGGGTC AGTCGGGTGT GACTATCAAG 
CACGTGGCCT GCGGGGACCT CTTCACCGCC TGCCTGACTG ACCGAGGCAT CATCATGACC 
TTTGGCAGTG GTAGCAACGG GTGCCTAGGC CATGGCGGCC TCAATGACAT CAGCCAGCCC 
ACCATTGTGG AGGCCCTGCT GGGCTATGAG ATGGTGCAGG TGGCTTGTGG GGCCTCTCAT 
GTGCTGGCCT TGTCCACTGA GCGAGAACTA TTTGCTTGGG GCCGCGGAGA TGGTGGTCGG 
CTGGGACTAG GCACCAGGGA GTCCCACAGC TGCCCCCAGC AGGTACCCAT GCCCCCAGGA 
CAGGAAGCCC AGAGGGTTAT ATGTGGCATT GACTCCTCTG TGATCCTCAC AGTGCCTGGC 
CAAGCCCTGG CCTGTGGGAG CAACAGGTTC AACAAGCTGG GCCTGGACCA CCTCTCCCTA 
GGGGAGGAGC CTGCCCTACA CCAGCAAGTG GAGGAGGCCC TGAACTTCAC TCCATTAGGT 
TCTGCACCCC TGGACCAGGA GCCCCTGCTG AGTGTGGATT TGGGCACTGC TCATTCAGCT 
GCTGTAACTG AAGGTGAAGT GTACTCCTGG GGCAAAGGGG CCCGAGGTCG CCTGGGAAGG 
AGAGATGAGG ACGCCGCACT CCCTAGGCCA GTGCAGCTGG ATGAGACCCA GCCCTACACA 
GTGACCTCTG TGTCCTGTTG CCATGGCAAC ACTCTCCTGG CTATCCGCCC TGTCACGGAT 
GAGCCAGTCC CCCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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NEK8 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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