niki cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	niki
Entrez Gene ID	42959
Full Name	nimA-like kinase
Synonyms	CG10951,Dmel\CG10951,NEK1,Niki
General protein information	Preferred Names nimA-like kinase Names CG10951 gene product from transcript CG10951-RA CG10951-PA niki-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 96A23-96A24

mRNA and Protein(s)

mRNA	Protein	Name
NM_143036.2	NP_651293.1	nimA-like kinase

Pathways from BioSystems

Homologies

	NEK8	XP_001106986.2
Drosophila melanogaster (fruit fly)	niki	NP_651293.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006872	XP_308885.4
Canis lupus familiaris (dog)	NEK8	XP_548291.3
Pan troglodytes (chimpanzee)	NEK8	XP_511801.3
Mus musculus (house mouse)	Nek8	NP_543125.1
Homo sapiens (human)	NEK8	NP_835464.1
Rattus norvegicus (Norway rat)	Nek8	NP_001099274.1
Danio rerio (zebrafish)	nek8	NP_620776.1
Bos taurus (cattle)	NEK8	NP_001179652.1
Gallus gallus (chicken)	NEK8	XP_415822.3
Caenorhabditis elegans (roundworm)	nekl-1	NP_490968.2
Xenopus tropicalis (tropical clawed frog)	nek8	NP_001006906.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following niki gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the niki cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa06024	NM_143036.2 Latest version!	Drosophila melanogaster nimA-like kinase (niki), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

niki ( NM_143036.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651293.1 Drosophila melanogaster nimA-like kinase (niki), partial mRNA.

Price & Availability↑

CloneID	OFa06024
Clone ID Related Accession (Same CDS sequence)	NM_143036.2
Accession Version	NM_143036.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2526bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nimA-like kinase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_143036.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on the 3' end.

ATGAAGAAGT TCCGGGCCAA GGCGAGCTCC CTGCCCATTT TCAACGGTCG CATAACCGAC 
GCCACCACGC TGACCACCAG TTCGCTGCAG TTACCCCTGG GTCAGAATAC GCAGCGGAAG 
CAATCGACTT GCACTCGAGT GCTGCCCACG GTTTTTACCA TTACGGACGG CACCACCGGA 
GCGGCGAGTA CCTCCTTAGC GGAGGCCATG TCGAGCAGTA AGGCACAGAT GCCCAACCGA 
CAGGAGAGCC TCCTGCAGCT TAGTGTTCCA CGGGAGACGG GAGTGGGAGT GGCAGGACCT 
GAATTGGCTA ACTATGAAAA GGTGCGGGTG GTGGGCCAGG GATCCTTTGG CATCGCCATC 
CTCTACCGAC GCAAGTCCGA CGGGCACCAG ATCGTCTTCA AGCAAATCAA CCTGAGTGAG 
TTGAGCCCGC CTGGTCGGGA TCTGGCTATG AACGAGGTTG ATGTCTTCTC GAAATTACAT 
CATCCGAATA TTGTGAGCTA CCTCGGTAGC TTCATCAAGG ACAACACTCT GCTTATCGAG 
ATGGAGTACG CCGATGGAGG TACCCTGGCC CAAATCATCG CCGAGCGGCA GGGAAAATTG 
CATTTTCCTG AACGATATAT AATAGCCGTA TTCGAACAGA TCTCCAGTGC CATCAACTAC 
ATGCACTCGG AGAACATCCT GCATCGGGAT TTGAAGACCG CGAATGTATT TCTGAACAGG 
CGAGGCATTG TGAAAATCGG AGATTTTGGC ATATCCAAAA TAATGAACAC CAAGATACAT 
GCCCAGACTG TTTTGGGCAC ACCCTACTAC TTCAGTCCGG AGATGTGCGA GGGTAAGGAG 
TACGACAACA AGAGTGACAT TTGGGCTCTG GGCTGCATTC TGGGCGAGAT GTGCTGTCTG 
AAGAAGACCT TTGCTGCCTC CAATCTATCC GAATTGGTAA CCAAGATTAT GGCAGGGAAC 
TATACACCAG TGCCATCGGG TTACACATCG GGACTTCGCA GTCTAATGTC CAATCTGCTT 
CAAGTGGAGG CACCACGGCG ACCTACCGCC TCGGAGGTAT TGGTCTACTG GATTCCACTG 
ATATTCCGTA GCCTGGGAAA GAACAAAGGG TACTCCTATG AGGATGATGT GGGAGGTCCG 
GGTAGCGACC AATTGACTGC TCCTGTTCCA GCCGCCGCCT ACAGCAATGT GTCCATGGAG 
TTGGAATTGC CCACCGCCCA GACGGAGACC AAACAATTGA TGATTGCGGA TACAGCGGCG 
CCGCACGAGA TCCTTGAGAA ACGATCCGTG CTATACCAGC TGAAGGCCTT CGGCACCTGC 
TTCAGCATGG CGCCCATCCA ACTGCCGCCG AAGGCTGTCA TCGTGGACGT TGCCATGTCG 
GATTCCCACT TCGTGGTGGT AAACGAGGAC GGATCAGCAT ACGCCTGGGG TGAGGGCACC 
CACGGGCAGC TGGGTCTCAC AGCTCTGGAG GCTTGGAAGC ACTACCCCAG TCGTATGGAG 
AGCGTGCGCA ACTACCATGT GGTGAGTGCC TGCGCCGGCG ATGGCTTCAC TATCCTGGTT 
ACCCAGGCGG GAAGTCTGCT AAGCTGCGGC AGCAATGCCC ATTTGGCACT GGGACAGGAC 
GAGCAACGCA ACTACCACAG CCCCAAGCTA ATAGCCCGAT TGGCAGATGT CCGGGTGGAG 
CAGGTGGCCG CTGGACTCCA GCACGTTCTG GCCTTGAGCC GAGAGGGAGC GGTCTACGTC 
TGGGGCACCA GTACGTGCGG TGCTCTTGGC CTTGGAAATT ATCAGCAGCA ACAGAAATTC 
CCCCAAAAGA TTCTGTTATC GCATGTGAAG ACCAAGCCAT CAAAGATTTA CTGCGGTCCT 
GATACTTCAG CTGTCTTATT TGCCAACGGG GAACTACACG TCTGTGGTAG CAATGACTAC 
AATAAACTCG GTTTCCAACG TTCGGCGAAG ATTACTGCCT TTAAAAAAGT CCAACTGCCT 
CACAAGGTGA CCCAGGCGTG CTTCTCGTCC ACCCATTCGG TGTTCCTGGT GGAGGGCGGC 
TATGTTTACA CCATGGGTCG AAATGCGGAG GGTCAGCGGG GAATCAGGCA CTGCAACTCG 
GTTGATCACC CCACCCTGGT GGATTCGGTT AAGTCTCGAT ATATTGTGAA GGCGAACTGT 
AGCGACCAGT GTACCATTGT CGCCTCGGAG GACAACATCA TCACCGTTTG GGGTACTCGT 
AATGGCCTCC CGGGGATTGG TTCGACCAAC TGTGGCCTGG GACTGCAGAT CTGCACACCC 
AACATGGAGC TGGAGTTGGG AAATAACACG GCAGCGTTTA CAAACTTTCT GGCCTCGGTC 
TACAAGTCGG AGCTGATACT GGAACCGGTG GATATATTAG CTCTCTTCTC TTCCAAGGAG 
CAGTGCGACC GTGGCTATTA CGTCCAGGTG CATGACGTTT ATCCTTTGGC ACATAGCGTT 
CTGGTGCTGG TAGACACGAC CACGCCACTA ATATCCTCAT ACGAAGGTGA TTACCCGCAC 
CTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651293.1
CDS	266..2791
Translation	MKKFRAKASSLPIFNGRITDATTLTTSSLQLPLGQNTQRKQSTCTRVLPTVFTITDGTTGAASTSLAEAMSSSKAQMPNRQESLLQLSVPRETGVGVAGPELANYEKVRVVGQGSFGIAILYRRKSDGHQIVFKQINLSELSPPGRDLAMNEVDVFSKLHHPNIVSYLGSFIKDNTLLIEMEYADGGTLAQIIAERQGKLHFPERYIIAVFEQISSAINYMHSENILHRDLKTANVFLNRRGIVKIGDFGISKIMNTKIHAQTVLGTPYYFSPEMCEGKEYDNKSDIWALGCILGEMCCLKKTFAASNLSELVTKIMAGNYTPVPSGYTSGLRSLMSNLLQVEAPRRPTASEVLVYWIPLIFRSLGKNKGYSYEDDVGGPGSDQLTAPVPAAAYSNVSMELELPTAQTETKQLMIADTAAPHEILEKRSVLYQLKAFGTCFSMAPIQLPPKAVIVDVAMSDSHFVVVNEDGSAYAWGEGTHGQLGLTALEAWKHYPSRMESVRNYHVVSACAGDGFTILVTQAGSLLSCGSNAHLALGQDEQRNYHSPKLIARLADVRVEQVAAGLQHVLALSREGAVYVWGTSTCGALGLGNYQQQQKFPQKILLSHVKTKPSKIYCGPDTSAVLFANGELHVCGSNDYNKLGFQRSAKITAFKKVQLPHKVTQACFSSTHSVFLVEGGYVYTMGRNAEGQRGIRHCNSVDHPTLVDSVKSRYIVKANCSDQCTIVASEDNIITVWGTRNGLPGIGSTNCGLGLQICTPNMELELGNNTAAFTNFLASVYKSELILEPVDILALFSSKEQCDRGYYVQVHDVYPLAHSVLVLVDTTTPLISSYEGDYPHL

Target ORF information:

RefSeq Version	NM_143036.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nimA-like kinase (niki), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143036.2

ORF Insert Sequence:

ATGAAGAAGT TCCGGGCCAA GGCGAGCTCC CTGCCCATTT TCAACGGTCG CATAACCGAC 
GCCACCACGC TGACCACCAG TTCGCTGCAG TTACCCCTGG GTCAGAATAC GCAGCGGAAG 
CAATCGACTT GCACTCGAGT GCTGCCCACG GTTTTTACCA TTACGGACGG CACCACCGGA 
GCGGCGAGTA CCTCCTTAGC GGAGGCCATG TCGAGCAGTA AGGCACAGAT GCCCAACCGA 
CAGGAGAGCC TCCTGCAGCT TAGTGTTCCA CGGGAGACGG GAGTGGGAGT GGCAGGACCT 
GAATTGGCTA ACTATGAAAA GGTGCGGGTG GTGGGCCAGG GATCCTTTGG CATCGCCATC 
CTCTACCGAC GCAAGTCCGA CGGGCACCAG ATCGTCTTCA AGCAAATCAA CCTGAGTGAG 
TTGAGCCCGC CTGGTCGGGA TCTGGCTATG AACGAGGTTG ATGTCTTCTC GAAATTACAT 
CATCCGAATA TTGTGAGCTA CCTCGGTAGC TTCATCAAGG ACAACACTCT GCTTATCGAG 
ATGGAGTACG CCGATGGAGG TACCCTGGCC CAAATCATCG CCGAGCGGCA GGGAAAATTG 
CATTTTCCTG AACGATATAT AATAGCCGTA TTCGAACAGA TCTCCAGTGC CATCAACTAC 
ATGCACTCGG AGAACATCCT GCATCGGGAT TTGAAGACCG CGAATGTATT TCTGAACAGG 
CGAGGCATTG TGAAAATCGG AGATTTTGGC ATATCCAAAA TAATGAACAC CAAGATACAT 
GCCCAGACTG TTTTGGGCAC ACCCTACTAC TTCAGTCCGG AGATGTGCGA GGGTAAGGAG 
TACGACAACA AGAGTGACAT TTGGGCTCTG GGCTGCATTC TGGGCGAGAT GTGCTGTCTG 
AAGAAGACCT TTGCTGCCTC CAATCTATCC GAATTGGTAA CCAAGATTAT GGCAGGGAAC 
TATACACCAG TGCCATCGGG TTACACATCG GGACTTCGCA GTCTAATGTC CAATCTGCTT 
CAAGTGGAGG CACCACGGCG ACCTACCGCC TCGGAGGTAT TGGTCTACTG GATTCCACTG 
ATATTCCGTA GCCTGGGAAA GAACAAAGGG TACTCCTATG AGGATGATGT GGGAGGTCCG 
GGTAGCGACC AATTGACTGC TCCTGTTCCA GCCGCCGCCT ACAGCAATGT GTCCATGGAG 
TTGGAATTGC CCACCGCCCA GACGGAGACC AAACAATTGA TGATTGCGGA TACAGCGGCG 
CCGCACGAGA TCCTTGAGAA ACGATCCGTG CTATACCAGC TGAAGGCCTT CGGCACCTGC 
TTCAGCATGG CGCCCATCCA ACTGCCGCCG AAGGCTGTCA TCGTGGACGT TGCCATGTCG 
GATTCCCACT TCGTGGTGGT AAACGAGGAC GGATCAGCAT ACGCCTGGGG TGAGGGCACC 
CACGGGCAGC TGGGTCTCAC AGCTCTGGAG GCTTGGAAGC ACTACCCCAG TCGTATGGAG 
AGCGTGCGCA ACTACCATGT GGTGAGTGCC TGCGCCGGCG ATGGCTTCAC TATCCTGGTT 
ACCCAGGCGG GAAGTCTGCT AAGCTGCGGC AGCAATGCCC ATTTGGCACT GGGACAGGAC 
GAGCAACGCA ACTACCACAG CCCCAAGCTA ATAGCCCGAT TGGCAGATGT CCGGGTGGAG 
CAGGTGGCCG CTGGACTCCA GCACGTTCTG GCCTTGAGCC GAGAGGGAGC GGTCTACGTC 
TGGGGCACCA GTACGTGCGG TGCTCTTGGC CTTGGAAATT ATCAGCAGCA ACAGAAATTC 
CCCCAAAAGA TTCTGTTATC GCATGTGAAG ACCAAGCCAT CAAAGATTTA CTGCGGTCCT 
GATACTTCAG CTGTCTTATT TGCCAACGGG GAACTACACG TCTGTGGTAG CAATGACTAC 
AATAAACTCG GTTTCCAACG TTCGGCGAAG ATTACTGCCT TTAAAAAAGT CCAACTGCCT 
CACAAGGTGA CCCAGGCGTG CTTCTCGTCC ACCCATTCGG TGTTCCTGGT GGAGGGCGGC 
TATGTTTACA CCATGGGTCG AAATGCGGAG GGTCAGCGGG GAATCAGGCA CTGCAACTCG 
GTTGATCACC CCACCCTGGT GGATTCGGTT AAGTCTCGAT ATATTGTGAA GGCGAACTGT 
AGCGACCAGT GTACCATTGT CGCCTCGGAG GACAACATCA TCACCGTTTG GGGTACTCGT 
AATGGCCTCC CGGGGATTGG TTCGACCAAC TGTGGCCTGG GACTGCAGAT CTGCACACCC 
AACATGGAGC TGGAGTTGGG AAATAACACG GCAGCGTTTA CAAACTTTCT GGCCTCGGTC 
TACAAGTCGG AGCTGATACT GGAACCGGTG GATATATTAG CTCTCTTCTC TTCCAAGGAG 
CAGTGCGACC GTGGCTATTA CGTCCAGGTG CATGACGTTT ATCCTTTGGC ACATAGCGTT 
CTGGTGCTGG TAGACACGAC CACGCCACTA ATATCCTCAT ACGAAGGTGA TTACCCGCAC 
CTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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niki cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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