NEK8 cDNA ORF clone, Pan troglodytes(chimpanzee)

Gene
Clones
gRNAs

Summary

Gene Symbol	NEK8
Entrez Gene ID	455033
Full Name	NIMA related kinase 8
General protein information	Preferred Names NIMA related kinase 8 Names serine/threonine-protein kinase Nek8 NIMA (never in mitosis gene a)- related kinase 8
Gene Type	protein-coding
Organism	Pan troglodytes(chimpanzee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_016932162.1	XP_016787651.1	serine/threonine-protein kinase Nek8 isoform X2
XM_016932161.2	XP_016787650.1	serine/threonine-protein kinase Nek8 isoform X1
XM_016932162.2	XP_016787651.1	serine/threonine-protein kinase Nek8 isoform X2
XM_016932163.2	XP_016787652.1	serine/threonine-protein kinase Nek8 isoform X3
XM_016932161.1	XP_016787650.1	serine/threonine-protein kinase Nek8 isoform X1
XM_016932163.1	XP_016787652.1	serine/threonine-protein kinase Nek8 isoform X3

Pathways from BioSystems

Homologies

	NEK8	XP_001106986.2
Drosophila melanogaster (fruit fly)	niki	NP_651293.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006872	XP_308885.4
Canis lupus familiaris (dog)	NEK8	XP_548291.3
Pan troglodytes (chimpanzee)	NEK8	XP_511801.3
Mus musculus (house mouse)	Nek8	NP_543125.1
Homo sapiens (human)	NEK8	NP_835464.1
Rattus norvegicus (Norway rat)	Nek8	NP_001099274.1
Danio rerio (zebrafish)	nek8	NP_620776.1
Bos taurus (cattle)	NEK8	NP_001179652.1
Gallus gallus (chicken)	NEK8	XP_415822.3
Caenorhabditis elegans (roundworm)	nekl-1	NP_490968.2
Xenopus tropicalis (tropical clawed frog)	nek8	NP_001006906.1

Gene Ontology

The following NEK8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NEK8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPc33489	XM_016932162.2 Latest version!	Pan troglodytes NIMA related kinase 8 (NEK8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OPc72784	XM_016932161.2 Latest version!	Pan troglodytes NIMA related kinase 8 (NEK8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OPc72785	XM_016932163.2 Latest version!	Pan troglodytes NIMA related kinase 8 (NEK8), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
View Old Accession Versions >>
OPc33489	XM_016932162.1	Pan troglodytes NIMA related kinase 8 (NEK8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OPc72784	XM_016932161.1	Pan troglodytes NIMA related kinase 8 (NEK8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OPc72785	XM_016932163.1	Pan troglodytes NIMA related kinase 8 (NEK8), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NEK8 ( XM_016932162.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016787651.1 Pan troglodytes NIMA related kinase 8 (NEK8), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPc33489
Clone ID Related Accession (Same CDS sequence)	XM_016932162.1 , XM_016932162.2
Accession Version	XM_016932162.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2079bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	serine/threonine-protein kinase Nek8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006484.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGT ACGAGCGGAT CCGAGTGGTG GGGAGAGGTG CCTTCGGGAT TGTGCACCTG 
TGCCTGCGAA AGGCTGACCA GAAGCTGGTG ATCATCAAGC AGATTCCAGT GGAACAGATG 
ACCAAGGAAG AGCGGCAGGC AGCCCAGAAT GAGTGCCAGG TCCTCAAGCT GCTCAACCAC 
CCCAATGTCA TTGAGTACTA CGAGAACTTC CTGGAAGACA AAGCCCTTAT GATCGCCATG 
GAATATGCAC CAGGCGGCAC TCTGGCTGAG TTCATCCAAA AGCGCTGTAA TTCCCTGCTG 
GAGGAGGAGA CCATCCTGCA CTTCTTCGTG CAGATCCTGC TTGCACTGCA TCATGTGCAC 
ACCCACCTCA TCCTGCACCG AGACCTCAAG ACCCAGAACA TCCTGCTTGA CAAACACCGC 
ATGGTCGTCA AGATCGGTGA TTTCGGCATC TCCAAGATCC TTAGCAGCAA GAGCAAGGCC 
TACACGGTGG TGGGTACCCC ATGCTATATC TCCCCTGAGC TGTGTGAGGG CAAGCCCTAC 
AACCAGAAGA GTGACATCTG GGCCCTGGGC TGTGTCCTCT ACGAGCTGGC CAGCCTCAAG 
AGGGCTTTCG AGGCTGCGAA CTTGCCAGCA CTGGTGCTGA AGATCATGAG TGGCACCTTT 
GCACCTATCT CTGACCGGTA CAGCCCTGAG CTTCGCCAGC TGGTCCTGAG TCTACTCAGC 
CTGGAGCCTG CCCAGCGGCC ACCACTCAGC CACATCATGG CACAGCCCCT CTGCATCCGT 
GCCCTCCTCA ACCTCCACAC CGACGTGGGC AGTGTCCGCA TGCGGAGGGC AGAGAAGTCC 
GTGGCCCCCA GCAGCACAGG GAGCAGGACC ACCAGTGTCC GCTGCAGAGG TATCCCCCGG 
GGACCTGTGA GGCCAGCCAT CCCACCACCA CTGTCGTCAG TGTATGCCTG GGGTGGTGGG 
CTGGGCACCC CCCTGCGGCT GCCAATGCTC AACACAGAGG TGGTCCAGGT GGCAGCTGGG 
CGCACGCAGA AAGCCGGCGT CACGCGCTCT GGGCGTCTCA TCCTGTGGGA GGCCCCACCC 
CTAGGTGCAG GCGGAGGCAG TCTCCTCCCT GGGGCAGTGG AGCAGCCACA GCCCCAGTTC 
ATCTCGCGTT TCCTGGAGGG CCAGTCGGGC GTGACCATCA AGCACGTGGC CTGTGGGGAC 
TTCTTCACTG CCTGCCTGAC TGACAGAGGC ATCATCATGA CATTCGGCAG CGGCAGCAAT 
GGGTGCCTAG GCCATGGCAG CCTCACTGAC ATCAGCCAGC CCACCATTGT GGAGGCTTTG 
CTGGGCTATG AAATGGTGCA GGTGGCCTGT GGGGCCTCTC ACGTGCTGGC CCTGTCCACT 
GAGCGAGAAC TATTTGCCTG GGGCCGTGGA GACAGCGGCA GACTGGGGCT AGGCACCAGG 
GAGTCCCACA GCTGCCCCCA GCAGGTGCCC ATGCCCCCAG GACAGGAAGC TCAGCGAGTT 
GTATGTGGCA TCGATTCCTC CATGATCCTC ACTGTGCCTG GCCAAGCCCT AGCCTGTGGG 
AGCAACAGGT TCAACAAGCT GGGCCTGGAC CACCTCTCCC TGGGGGAGGA GCCTGTCCCC 
CACCAGCAAG TGGAGGAGGC CCTGAGCTTC ACACTACTAG GCTCTGCACC CCTGGACCAG 
GAGCCTCTGC TGAGTATAGA CCTGGGCACT GCTCACTCAG CTGCTGTGAC TGCCTCGGGT 
GATTGCTACA CTTTTGGCAG CAATCAGCAC GGACAGTTGG GCACCAATAC TCGCCGAGGC 
AGTCGGGCAC CCTGTAAGGT CCAAGGCCTT GAGGGCATCA AGATGGCAAT GGTAGCCTGT 
GGGGATGCCT TCACTGTAGC TATTGGGGCA GAGGGCGAAG TGTACTCTTG GGGCAAAGGG 
GCGCGAGGTC GATTGGGAAG GAGGGATGAG GATGCCGGAC TCCCTCGGCC AGTGCAGTTG 
GATGAGACAC ACCCTTACAC GGTGACTTCC GTGTCCTGTT GCCATGGAAA CACCCTCCTG 
GCTGTTCGAT CGGTCACAGA TGAGCCGGTC CCCCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016787651.1
CDS	465..2543
Translation	MEKYERIRVVGRGAFGIVHLCLRKADQKLVIIKQIPVEQMTKEERQAAQNECQVLKLLNHPNVIEYYENFLEDKALMIAMEYAPGGTLAEFIQKRCNSLLEEETILHFFVQILLALHHVHTHLILHRDLKTQNILLDKHRMVVKIGDFGISKILSSKSKAYTVVGTPCYISPELCEGKPYNQKSDIWALGCVLYELASLKRAFEAANLPALVLKIMSGTFAPISDRYSPELRQLVLSLLSLEPAQRPPLSHIMAQPLCIRALLNLHTDVGSVRMRRAEKSVAPSSTGSRTTSVRCRGIPRGPVRPAIPPPLSSVYAWGGGLGTPLRLPMLNTEVVQVAAGRTQKAGVTRSGRLILWEAPPLGAGGGSLLPGAVEQPQPQFISRFLEGQSGVTIKHVACGDFFTACLTDRGIIMTFGSGSNGCLGHGSLTDISQPTIVEALLGYEMVQVACGASHVLALSTERELFAWGRGDSGRLGLGTRESHSCPQQVPMPPGQEAQRVVCGIDSSMILTVPGQALACGSNRFNKLGLDHLSLGEEPVPHQQVEEALSFTLLGSAPLDQEPLLSIDLGTAHSAAVTASGDCYTFGSNQHGQLGTNTRRGSRAPCKVQGLEGIKMAMVACGDAFTVAIGAEGEVYSWGKGARGRLGRRDEDAGLPRPVQLDETHPYTVTSVSCCHGNTLLAVRSVTDEPVPP

Target ORF information:

RefSeq Version	XM_016932162.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes NIMA related kinase 8 (NEK8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016932162.1

ORF Insert Sequence:

ATGGAGAAGT ACGAGCGGAT CCGAGTGGTG GGGAGAGGTG CCTTCGGGAT TGTGCACCTG 
TGCCTGCGAA AGGCTGACCA GAAGCTGGTG ATCATCAAGC AGATTCCAGT GGAACAGATG 
ACCAAGGAAG AGCGGCAGGC AGCCCAGAAT GAGTGCCAGG TCCTCAAGCT GCTCAACCAC 
CCCAATGTCA TTGAGTACTA CGAGAACTTC CTGGAAGACA AAGCCCTTAT GATCGCCATG 
GAATATGCAC CAGGCGGCAC TCTGGCTGAG TTCATCCAAA AGCGCTGTAA TTCCCTGCTG 
GAGGAGGAGA CCATCCTGCA CTTCTTCGTG CAGATCCTGC TTGCACTGCA TCATGTGCAC 
ACCCACCTCA TCCTGCACCG AGACCTCAAG ACCCAGAACA TCCTGCTTGA CAAACACCGC 
ATGGTCGTCA AGATCGGTGA TTTCGGCATC TCCAAGATCC TTAGCAGCAA GAGCAAGGCC 
TACACGGTGG TGGGTACCCC ATGCTATATC TCCCCTGAGC TGTGTGAGGG CAAGCCCTAC 
AACCAGAAGA GTGACATCTG GGCCCTGGGC TGTGTCCTCT ACGAGCTGGC CAGCCTCAAG 
AGGGCTTTCG AGGCTGCGAA CTTGCCAGCA CTGGTGCTGA AGATCATGAG TGGCACCTTT 
GCACCTATCT CTGACCGGTA CAGCCCTGAG CTTCGCCAGC TGGTCCTGAG TCTACTCAGC 
CTGGAGCCTG CCCAGCGGCC ACCACTCAGC CACATCATGG CACAGCCCCT CTGCATCCGT 
GCCCTCCTCA ACCTCCACAC CGACGTGGGC AGTGTCCGCA TGCGGAGGGC AGAGAAGTCC 
GTGGCCCCCA GCAGCACAGG GAGCAGGACC ACCAGTGTCC GCTGCAGAGG TATCCCCCGG 
GGACCTGTGA GGCCAGCCAT CCCACCACCA CTGTCGTCAG TGTATGCCTG GGGTGGTGGG 
CTGGGCACCC CCCTGCGGCT GCCAATGCTC AACACAGAGG TGGTCCAGGT GGCAGCTGGG 
CGCACGCAGA AAGCCGGCGT CACGCGCTCT GGGCGTCTCA TCCTGTGGGA GGCCCCACCC 
CTAGGTGCAG GCGGAGGCAG TCTCCTCCCT GGGGCAGTGG AGCAGCCACA GCCCCAGTTC 
ATCTCGCGTT TCCTGGAGGG CCAGTCGGGC GTGACCATCA AGCACGTGGC CTGTGGGGAC 
TTCTTCACTG CCTGCCTGAC TGACAGAGGC ATCATCATGA CATTCGGCAG CGGCAGCAAT 
GGGTGCCTAG GCCATGGCAG CCTCACTGAC ATCAGCCAGC CCACCATTGT GGAGGCTTTG 
CTGGGCTATG AAATGGTGCA GGTGGCCTGT GGGGCCTCTC ACGTGCTGGC CCTGTCCACT 
GAGCGAGAAC TATTTGCCTG GGGCCGTGGA GACAGCGGCA GACTGGGGCT AGGCACCAGG 
GAGTCCCACA GCTGCCCCCA GCAGGTGCCC ATGCCCCCAG GACAGGAAGC TCAGCGAGTT 
GTATGTGGCA TCGATTCCTC CATGATCCTC ACTGTGCCTG GCCAAGCCCT AGCCTGTGGG 
AGCAACAGGT TCAACAAGCT GGGCCTGGAC CACCTCTCCC TGGGGGAGGA GCCTGTCCCC 
CACCAGCAAG TGGAGGAGGC CCTGAGCTTC ACACTACTAG GCTCTGCACC CCTGGACCAG 
GAGCCTCTGC TGAGTATAGA CCTGGGCACT GCTCACTCAG CTGCTGTGAC TGCCTCGGGT 
GATTGCTACA CTTTTGGCAG CAATCAGCAC GGACAGTTGG GCACCAATAC TCGCCGAGGC 
AGTCGGGCAC CCTGTAAGGT CCAAGGCCTT GAGGGCATCA AGATGGCAAT GGTAGCCTGT 
GGGGATGCCT TCACTGTAGC TATTGGGGCA GAGGGCGAAG TGTACTCTTG GGGCAAAGGG 
GCGCGAGGTC GATTGGGAAG GAGGGATGAG GATGCCGGAC TCCCTCGGCC AGTGCAGTTG 
GATGAGACAC ACCCTTACAC GGTGACTTCC GTGTCCTGTT GCCATGGAAA CACCCTCCTG 
GCTGTTCGAT CGGTCACAGA TGAGCCGGTC CCCCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK8 ( XM_016932162.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016787651.1 Pan troglodytes NIMA related kinase 8 (NEK8), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPc33489
Clone ID Related Accession (Same CDS sequence)	XM_016932162.1 , XM_016932162.2
Accession Version	XM_016932162.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2079bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	serine/threonine-protein kinase Nek8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036896.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016932162.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGT ACGAGCGGAT CCGAGTGGTG GGGAGAGGTG CCTTCGGGAT TGTGCACCTG 
TGCCTGCGAA AGGCTGACCA GAAGCTGGTG ATCATCAAGC AGATTCCAGT GGAACAGATG 
ACCAAGGAAG AGCGGCAGGC AGCCCAGAAT GAGTGCCAGG TCCTCAAGCT GCTCAACCAC 
CCCAATGTCA TTGAGTACTA CGAGAACTTC CTGGAAGACA AAGCCCTTAT GATCGCCATG 
GAATATGCAC CAGGCGGCAC TCTGGCTGAG TTCATCCAAA AGCGCTGTAA TTCCCTGCTG 
GAGGAGGAGA CCATCCTGCA CTTCTTCGTG CAGATCCTGC TTGCACTGCA TCATGTGCAC 
ACCCACCTCA TCCTGCACCG AGACCTCAAG ACCCAGAACA TCCTGCTTGA CAAACACCGC 
ATGGTCGTCA AGATCGGTGA TTTCGGCATC TCCAAGATCC TTAGCAGCAA GAGCAAGGCC 
TACACGGTGG TGGGTACCCC ATGCTATATC TCCCCTGAGC TGTGTGAGGG CAAGCCCTAC 
AACCAGAAGA GTGACATCTG GGCCCTGGGC TGTGTCCTCT ACGAGCTGGC CAGCCTCAAG 
AGGGCTTTCG AGGCTGCGAA CTTGCCAGCA CTGGTGCTGA AGATCATGAG TGGCACCTTT 
GCACCTATCT CTGACCGGTA CAGCCCTGAG CTTCGCCAGC TGGTCCTGAG TCTACTCAGC 
CTGGAGCCTG CCCAGCGGCC ACCACTCAGC CACATCATGG CACAGCCCCT CTGCATCCGT 
GCCCTCCTCA ACCTCCACAC CGACGTGGGC AGTGTCCGCA TGCGGAGGGC AGAGAAGTCC 
GTGGCCCCCA GCAGCACAGG GAGCAGGACC ACCAGTGTCC GCTGCAGAGG TATCCCCCGG 
GGACCTGTGA GGCCAGCCAT CCCACCACCA CTGTCGTCAG TGTATGCCTG GGGTGGTGGG 
CTGGGCACCC CCCTGCGGCT GCCAATGCTC AACACAGAGG TGGTCCAGGT GGCAGCTGGG 
CGCACGCAGA AAGCCGGCGT CACGCGCTCT GGGCGTCTCA TCCTGTGGGA GGCCCCACCC 
CTAGGTGCAG GCGGAGGCAG TCTCCTCCCT GGGGCAGTGG AGCAGCCACA GCCCCAGTTC 
ATCTCGCGTT TCCTGGAGGG CCAGTCGGGC GTGACCATCA AGCACGTGGC CTGTGGGGAC 
TTCTTCACTG CCTGCCTGAC TGACAGAGGC ATCATCATGA CATTCGGCAG CGGCAGCAAT 
GGGTGCCTAG GCCATGGCAG CCTCACTGAC ATCAGCCAGC CCACCATTGT GGAGGCTTTG 
CTGGGCTATG AAATGGTGCA GGTGGCCTGT GGGGCCTCTC ACGTGCTGGC CCTGTCCACT 
GAGCGAGAAC TATTTGCCTG GGGCCGTGGA GACAGCGGCA GACTGGGGCT AGGCACCAGG 
GAGTCCCACA GCTGCCCCCA GCAGGTGCCC ATGCCCCCAG GACAGGAAGC TCAGCGAGTT 
GTATGTGGCA TCGATTCCTC CATGATCCTC ACTGTGCCTG GCCAAGCCCT AGCCTGTGGG 
AGCAACAGGT TCAACAAGCT GGGCCTGGAC CACCTCTCCC TGGGGGAGGA GCCTGTCCCC 
CACCAGCAAG TGGAGGAGGC CCTGAGCTTC ACACTACTAG GCTCTGCACC CCTGGACCAG 
GAGCCTCTGC TGAGTATAGA CCTGGGCACT GCTCACTCAG CTGCTGTGAC TGCCTCGGGT 
GATTGCTACA CTTTTGGCAG CAATCAGCAC GGACAGTTGG GCACCAATAC TCGCCGAGGC 
AGTCGGGCAC CCTGTAAGGT CCAAGGCCTT GAGGGCATCA AGATGGCAAT GGTAGCCTGT 
GGGGATGCCT TCACTGTAGC TATTGGGGCA GAGGGCGAAG TGTACTCTTG GGGCAAAGGG 
GCGCGAGGTC GATTGGGAAG GAGGGATGAG GATGCCGGAC TCCCTCGGCC AGTGCAGTTG 
GATGAGACAC ACCCTTACAC GGTGACTTCC GTGTCCTGTT GCCATGGAAA CACCCTCCTG 
GCTGTTCGAT CGGTCACAGA TGAGCCGGTC CCCCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016787651.1
CDS	463..2541
Translation	MEKYERIRVVGRGAFGIVHLCLRKADQKLVIIKQIPVEQMTKEERQAAQNECQVLKLLNHPNVIEYYENFLEDKALMIAMEYAPGGTLAEFIQKRCNSLLEEETILHFFVQILLALHHVHTHLILHRDLKTQNILLDKHRMVVKIGDFGISKILSSKSKAYTVVGTPCYISPELCEGKPYNQKSDIWALGCVLYELASLKRAFEAANLPALVLKIMSGTFAPISDRYSPELRQLVLSLLSLEPAQRPPLSHIMAQPLCIRALLNLHTDVGSVRMRRAEKSVAPSSTGSRTTSVRCRGIPRGPVRPAIPPPLSSVYAWGGGLGTPLRLPMLNTEVVQVAAGRTQKAGVTRSGRLILWEAPPLGAGGGSLLPGAVEQPQPQFISRFLEGQSGVTIKHVACGDFFTACLTDRGIIMTFGSGSNGCLGHGSLTDISQPTIVEALLGYEMVQVACGASHVLALSTERELFAWGRGDSGRLGLGTRESHSCPQQVPMPPGQEAQRVVCGIDSSMILTVPGQALACGSNRFNKLGLDHLSLGEEPVPHQQVEEALSFTLLGSAPLDQEPLLSIDLGTAHSAAVTASGDCYTFGSNQHGQLGTNTRRGSRAPCKVQGLEGIKMAMVACGDAFTVAIGAEGEVYSWGKGARGRLGRRDEDAGLPRPVQLDETHPYTVTSVSCCHGNTLLAVRSVTDEPVPP

Target ORF information:

RefSeq Version	XM_016932162.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes NIMA related kinase 8 (NEK8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016932162.2

ORF Insert Sequence:

ATGGAGAAGT ACGAGCGGAT CCGAGTGGTG GGGAGAGGTG CCTTCGGGAT TGTGCACCTG 
TGCCTGCGAA AGGCTGACCA GAAGCTGGTG ATCATCAAGC AGATTCCAGT GGAACAGATG 
ACCAAGGAAG AGCGGCAGGC AGCCCAGAAT GAGTGCCAGG TCCTCAAGCT GCTCAACCAC 
CCCAATGTCA TTGAGTACTA CGAGAACTTC CTGGAAGACA AAGCCCTTAT GATCGCCATG 
GAATATGCAC CAGGCGGCAC TCTGGCTGAG TTCATCCAAA AGCGCTGTAA TTCCCTGCTG 
GAGGAGGAGA CCATCCTGCA CTTCTTCGTG CAGATCCTGC TTGCACTGCA TCATGTGCAC 
ACCCACCTCA TCCTGCACCG AGACCTCAAG ACCCAGAACA TCCTGCTTGA CAAACACCGC 
ATGGTCGTCA AGATCGGTGA TTTCGGCATC TCCAAGATCC TTAGCAGCAA GAGCAAGGCC 
TACACGGTGG TGGGTACCCC ATGCTATATC TCCCCTGAGC TGTGTGAGGG CAAGCCCTAC 
AACCAGAAGA GTGACATCTG GGCCCTGGGC TGTGTCCTCT ACGAGCTGGC CAGCCTCAAG 
AGGGCTTTCG AGGCTGCGAA CTTGCCAGCA CTGGTGCTGA AGATCATGAG TGGCACCTTT 
GCACCTATCT CTGACCGGTA CAGCCCTGAG CTTCGCCAGC TGGTCCTGAG TCTACTCAGC 
CTGGAGCCTG CCCAGCGGCC ACCACTCAGC CACATCATGG CACAGCCCCT CTGCATCCGT 
GCCCTCCTCA ACCTCCACAC CGACGTGGGC AGTGTCCGCA TGCGGAGGGC AGAGAAGTCC 
GTGGCCCCCA GCAGCACAGG GAGCAGGACC ACCAGTGTCC GCTGCAGAGG TATCCCCCGG 
GGACCTGTGA GGCCAGCCAT CCCACCACCA CTGTCGTCAG TGTATGCCTG GGGTGGTGGG 
CTGGGCACCC CCCTGCGGCT GCCAATGCTC AACACAGAGG TGGTCCAGGT GGCAGCTGGG 
CGCACGCAGA AAGCCGGCGT CACGCGCTCT GGGCGTCTCA TCCTGTGGGA GGCCCCACCC 
CTAGGTGCAG GCGGAGGCAG TCTCCTCCCT GGGGCAGTGG AGCAGCCACA GCCCCAGTTC 
ATCTCGCGTT TCCTGGAGGG CCAGTCGGGC GTGACCATCA AGCACGTGGC CTGTGGGGAC 
TTCTTCACTG CCTGCCTGAC TGACAGAGGC ATCATCATGA CATTCGGCAG CGGCAGCAAT 
GGGTGCCTAG GCCATGGCAG CCTCACTGAC ATCAGCCAGC CCACCATTGT GGAGGCTTTG 
CTGGGCTATG AAATGGTGCA GGTGGCCTGT GGGGCCTCTC ACGTGCTGGC CCTGTCCACT 
GAGCGAGAAC TATTTGCCTG GGGCCGTGGA GACAGCGGCA GACTGGGGCT AGGCACCAGG 
GAGTCCCACA GCTGCCCCCA GCAGGTGCCC ATGCCCCCAG GACAGGAAGC TCAGCGAGTT 
GTATGTGGCA TCGATTCCTC CATGATCCTC ACTGTGCCTG GCCAAGCCCT AGCCTGTGGG 
AGCAACAGGT TCAACAAGCT GGGCCTGGAC CACCTCTCCC TGGGGGAGGA GCCTGTCCCC 
CACCAGCAAG TGGAGGAGGC CCTGAGCTTC ACACTACTAG GCTCTGCACC CCTGGACCAG 
GAGCCTCTGC TGAGTATAGA CCTGGGCACT GCTCACTCAG CTGCTGTGAC TGCCTCGGGT 
GATTGCTACA CTTTTGGCAG CAATCAGCAC GGACAGTTGG GCACCAATAC TCGCCGAGGC 
AGTCGGGCAC CCTGTAAGGT CCAAGGCCTT GAGGGCATCA AGATGGCAAT GGTAGCCTGT 
GGGGATGCCT TCACTGTAGC TATTGGGGCA GAGGGCGAAG TGTACTCTTG GGGCAAAGGG 
GCGCGAGGTC GATTGGGAAG GAGGGATGAG GATGCCGGAC TCCCTCGGCC AGTGCAGTTG 
GATGAGACAC ACCCTTACAC GGTGACTTCC GTGTCCTGTT GCCATGGAAA CACCCTCCTG 
GCTGTTCGAT CGGTCACAGA TGAGCCGGTC CCCCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK8 ( XM_016932161.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016787650.1 Pan troglodytes NIMA related kinase 8 (NEK8), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPc72784
Clone ID Related Accession (Same CDS sequence)	XM_016932161.2 , XM_016932161.1
Accession Version	XM_016932161.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2151bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	serine/threonine-protein kinase Nek8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036896.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016932161.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCACTG AGGGCAGATT ACCCTCCTGC AGCGCGTGTG TGAAAGGGGA GTTGAGAGTG 
CTGACGAGCG CGGCGCTCAC TAGTCGGGAC GGCCCGAGAC CGTGTCATGT CCTCTTCAGG 
ATTGTGCACC TGTGCCTGCG AAAGGCTGAC CAGAAGCTGG TGATCATCAA GCAGATTCCA 
GTGGAACAGA TGACCAAGGA AGAGCGGCAG GCAGCCCAGA ATGAGTGCCA GGTCCTCAAG 
CTGCTCAACC ACCCCAATGT CATTGAGTAC TACGAGAACT TCCTGGAAGA CAAAGCCCTT 
ATGATCGCCA TGGAATATGC ACCAGGCGGC ACTCTGGCTG AGTTCATCCA AAAGCGCTGT 
AATTCCCTGC TGGAGGAGGA GACCATCCTG CACTTCTTCG TGCAGATCCT GCTTGCACTG 
CATCATGTGC ACACCCACCT CATCCTGCAC CGAGACCTCA AGACCCAGAA CATCCTGCTT 
GACAAACACC GCATGGTCGT CAAGATCGGT GATTTCGGCA TCTCCAAGAT CCTTAGCAGC 
AAGAGCAAGG CCTACACGGT GGTGGGTACC CCATGCTATA TCTCCCCTGA GCTGTGTGAG 
GGCAAGCCCT ACAACCAGAA GAGTGACATC TGGGCCCTGG GCTGTGTCCT CTACGAGCTG 
GCCAGCCTCA AGAGGGCTTT CGAGGCTGCG AACTTGCCAG CACTGGTGCT GAAGATCATG 
AGTGGCACCT TTGCACCTAT CTCTGACCGG TACAGCCCTG AGCTTCGCCA GCTGGTCCTG 
AGTCTACTCA GCCTGGAGCC TGCCCAGCGG CCACCACTCA GCCACATCAT GGCACAGCCC 
CTCTGCATCC GTGCCCTCCT CAACCTCCAC ACCGACGTGG GCAGTGTCCG CATGCGGAGG 
GCAGAGAAGT CCGTGGCCCC CAGCAGCACA GGGAGCAGGA CCACCAGTGT CCGCTGCAGA 
GGTATCCCCC GGGGACCTGT GAGGCCAGCC ATCCCACCAC CACTGTCGTC AGTGTATGCC 
TGGGGTGGTG GGCTGGGCAC CCCCCTGCGG CTGCCAATGC TCAACACAGA GGTGGTCCAG 
GTGGCAGCTG GGCGCACGCA GAAAGCCGGC GTCACGCGCT CTGGGCGTCT CATCCTGTGG 
GAGGCCCCAC CCCTAGGTGC AGGCGGAGGC AGTCTCCTCC CTGGGGCAGT GGAGCAGCCA 
CAGCCCCAGT TCATCTCGCG TTTCCTGGAG GGCCAGTCGG GCGTGACCAT CAAGCACGTG 
GCCTGTGGGG ACTTCTTCAC TGCCTGCCTG ACTGACAGAG GCATCATCAT GACATTCGGC 
AGCGGCAGCA ATGGGTGCCT AGGCCATGGC AGCCTCACTG ACATCAGCCA GCCCACCATT 
GTGGAGGCTT TGCTGGGCTA TGAAATGGTG CAGGTGGCCT GTGGGGCCTC TCACGTGCTG 
GCCCTGTCCA CTGAGCGAGA ACTATTTGCC TGGGGCCGTG GAGACAGCGG CAGACTGGGG 
CTAGGCACCA GGGAGTCCCA CAGCTGCCCC CAGCAGGTGC CCATGCCCCC AGGACAGGAA 
GCTCAGCGAG TTGTATGTGG CATCGATTCC TCCATGATCC TCACTGTGCC TGGCCAAGCC 
CTAGCCTGTG GGAGCAACAG GTTCAACAAG CTGGGCCTGG ACCACCTCTC CCTGGGGGAG 
GAGCCTGTCC CCCACCAGCA AGTGGAGGAG GCCCTGAGCT TCACACTACT AGGCTCTGCA 
CCCCTGGACC AGGAGCCTCT GCTGAGTATA GACCTGGGCA CTGCTCACTC AGCTGCTGTG 
ACTGCCTCGG GTGATTGCTA CACTTTTGGC AGCAATCAGC ACGGACAGTT GGGCACCAAT 
ACTCGCCGAG GCAGTCGGGC ACCCTGTAAG GTCCAAGGCC TTGAGGGCAT CAAGATGGCA 
ATGGTAGCCT GTGGGGATGC CTTCACTGTA GCTATTGGGG CAGAGGGCGA AGTGTACTCT 
TGGGGCAAAG GGGCGCGAGG TCGATTGGGA AGGAGGGATG AGGATGCCGG ACTCCCTCGG 
CCAGTGCAGT TGGATGAGAC ACACCCTTAC ACGGTGACTT CCGTGTCCTG TTGCCATGGA 
AACACCCTCC TGGCTGTTCG ATCGGTCACA GATGAGCCGG TCCCCCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016787650.1
CDS	35..2185
Translation	MSTEGRLPSCSACVKGELRVLTSAALTSRDGPRPCHVLFRIVHLCLRKADQKLVIIKQIPVEQMTKEERQAAQNECQVLKLLNHPNVIEYYENFLEDKALMIAMEYAPGGTLAEFIQKRCNSLLEEETILHFFVQILLALHHVHTHLILHRDLKTQNILLDKHRMVVKIGDFGISKILSSKSKAYTVVGTPCYISPELCEGKPYNQKSDIWALGCVLYELASLKRAFEAANLPALVLKIMSGTFAPISDRYSPELRQLVLSLLSLEPAQRPPLSHIMAQPLCIRALLNLHTDVGSVRMRRAEKSVAPSSTGSRTTSVRCRGIPRGPVRPAIPPPLSSVYAWGGGLGTPLRLPMLNTEVVQVAAGRTQKAGVTRSGRLILWEAPPLGAGGGSLLPGAVEQPQPQFISRFLEGQSGVTIKHVACGDFFTACLTDRGIIMTFGSGSNGCLGHGSLTDISQPTIVEALLGYEMVQVACGASHVLALSTERELFAWGRGDSGRLGLGTRESHSCPQQVPMPPGQEAQRVVCGIDSSMILTVPGQALACGSNRFNKLGLDHLSLGEEPVPHQQVEEALSFTLLGSAPLDQEPLLSIDLGTAHSAAVTASGDCYTFGSNQHGQLGTNTRRGSRAPCKVQGLEGIKMAMVACGDAFTVAIGAEGEVYSWGKGARGRLGRRDEDAGLPRPVQLDETHPYTVTSVSCCHGNTLLAVRSVTDEPVPP

Target ORF information:

RefSeq Version	XM_016932161.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes NIMA related kinase 8 (NEK8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016932161.2

ORF Insert Sequence:

ATGAGCACTG AGGGCAGATT ACCCTCCTGC AGCGCGTGTG TGAAAGGGGA GTTGAGAGTG 
CTGACGAGCG CGGCGCTCAC TAGTCGGGAC GGCCCGAGAC CGTGTCATGT CCTCTTCAGG 
ATTGTGCACC TGTGCCTGCG AAAGGCTGAC CAGAAGCTGG TGATCATCAA GCAGATTCCA 
GTGGAACAGA TGACCAAGGA AGAGCGGCAG GCAGCCCAGA ATGAGTGCCA GGTCCTCAAG 
CTGCTCAACC ACCCCAATGT CATTGAGTAC TACGAGAACT TCCTGGAAGA CAAAGCCCTT 
ATGATCGCCA TGGAATATGC ACCAGGCGGC ACTCTGGCTG AGTTCATCCA AAAGCGCTGT 
AATTCCCTGC TGGAGGAGGA GACCATCCTG CACTTCTTCG TGCAGATCCT GCTTGCACTG 
CATCATGTGC ACACCCACCT CATCCTGCAC CGAGACCTCA AGACCCAGAA CATCCTGCTT 
GACAAACACC GCATGGTCGT CAAGATCGGT GATTTCGGCA TCTCCAAGAT CCTTAGCAGC 
AAGAGCAAGG CCTACACGGT GGTGGGTACC CCATGCTATA TCTCCCCTGA GCTGTGTGAG 
GGCAAGCCCT ACAACCAGAA GAGTGACATC TGGGCCCTGG GCTGTGTCCT CTACGAGCTG 
GCCAGCCTCA AGAGGGCTTT CGAGGCTGCG AACTTGCCAG CACTGGTGCT GAAGATCATG 
AGTGGCACCT TTGCACCTAT CTCTGACCGG TACAGCCCTG AGCTTCGCCA GCTGGTCCTG 
AGTCTACTCA GCCTGGAGCC TGCCCAGCGG CCACCACTCA GCCACATCAT GGCACAGCCC 
CTCTGCATCC GTGCCCTCCT CAACCTCCAC ACCGACGTGG GCAGTGTCCG CATGCGGAGG 
GCAGAGAAGT CCGTGGCCCC CAGCAGCACA GGGAGCAGGA CCACCAGTGT CCGCTGCAGA 
GGTATCCCCC GGGGACCTGT GAGGCCAGCC ATCCCACCAC CACTGTCGTC AGTGTATGCC 
TGGGGTGGTG GGCTGGGCAC CCCCCTGCGG CTGCCAATGC TCAACACAGA GGTGGTCCAG 
GTGGCAGCTG GGCGCACGCA GAAAGCCGGC GTCACGCGCT CTGGGCGTCT CATCCTGTGG 
GAGGCCCCAC CCCTAGGTGC AGGCGGAGGC AGTCTCCTCC CTGGGGCAGT GGAGCAGCCA 
CAGCCCCAGT TCATCTCGCG TTTCCTGGAG GGCCAGTCGG GCGTGACCAT CAAGCACGTG 
GCCTGTGGGG ACTTCTTCAC TGCCTGCCTG ACTGACAGAG GCATCATCAT GACATTCGGC 
AGCGGCAGCA ATGGGTGCCT AGGCCATGGC AGCCTCACTG ACATCAGCCA GCCCACCATT 
GTGGAGGCTT TGCTGGGCTA TGAAATGGTG CAGGTGGCCT GTGGGGCCTC TCACGTGCTG 
GCCCTGTCCA CTGAGCGAGA ACTATTTGCC TGGGGCCGTG GAGACAGCGG CAGACTGGGG 
CTAGGCACCA GGGAGTCCCA CAGCTGCCCC CAGCAGGTGC CCATGCCCCC AGGACAGGAA 
GCTCAGCGAG TTGTATGTGG CATCGATTCC TCCATGATCC TCACTGTGCC TGGCCAAGCC 
CTAGCCTGTG GGAGCAACAG GTTCAACAAG CTGGGCCTGG ACCACCTCTC CCTGGGGGAG 
GAGCCTGTCC CCCACCAGCA AGTGGAGGAG GCCCTGAGCT TCACACTACT AGGCTCTGCA 
CCCCTGGACC AGGAGCCTCT GCTGAGTATA GACCTGGGCA CTGCTCACTC AGCTGCTGTG 
ACTGCCTCGG GTGATTGCTA CACTTTTGGC AGCAATCAGC ACGGACAGTT GGGCACCAAT 
ACTCGCCGAG GCAGTCGGGC ACCCTGTAAG GTCCAAGGCC TTGAGGGCAT CAAGATGGCA 
ATGGTAGCCT GTGGGGATGC CTTCACTGTA GCTATTGGGG CAGAGGGCGA AGTGTACTCT 
TGGGGCAAAG GGGCGCGAGG TCGATTGGGA AGGAGGGATG AGGATGCCGG ACTCCCTCGG 
CCAGTGCAGT TGGATGAGAC ACACCCTTAC ACGGTGACTT CCGTGTCCTG TTGCCATGGA 
AACACCCTCC TGGCTGTTCG ATCGGTCACA GATGAGCCGG TCCCCCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK8 ( XM_016932161.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016787650.1 Pan troglodytes NIMA related kinase 8 (NEK8), transcript variant X1, mRNA.

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CloneID	OPc72784
Clone ID Related Accession (Same CDS sequence)	XM_016932161.2 , XM_016932161.1
Accession Version	XM_016932161.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2151bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	serine/threonine-protein kinase Nek8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006484.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCACTG AGGGCAGATT ACCCTCCTGC AGCGCGTGTG TGAAAGGGGA GTTGAGAGTG 
CTGACGAGCG CGGCGCTCAC TAGTCGGGAC GGCCCGAGAC CGTGTCATGT CCTCTTCAGG 
ATTGTGCACC TGTGCCTGCG AAAGGCTGAC CAGAAGCTGG TGATCATCAA GCAGATTCCA 
GTGGAACAGA TGACCAAGGA AGAGCGGCAG GCAGCCCAGA ATGAGTGCCA GGTCCTCAAG 
CTGCTCAACC ACCCCAATGT CATTGAGTAC TACGAGAACT TCCTGGAAGA CAAAGCCCTT 
ATGATCGCCA TGGAATATGC ACCAGGCGGC ACTCTGGCTG AGTTCATCCA AAAGCGCTGT 
AATTCCCTGC TGGAGGAGGA GACCATCCTG CACTTCTTCG TGCAGATCCT GCTTGCACTG 
CATCATGTGC ACACCCACCT CATCCTGCAC CGAGACCTCA AGACCCAGAA CATCCTGCTT 
GACAAACACC GCATGGTCGT CAAGATCGGT GATTTCGGCA TCTCCAAGAT CCTTAGCAGC 
AAGAGCAAGG CCTACACGGT GGTGGGTACC CCATGCTATA TCTCCCCTGA GCTGTGTGAG 
GGCAAGCCCT ACAACCAGAA GAGTGACATC TGGGCCCTGG GCTGTGTCCT CTACGAGCTG 
GCCAGCCTCA AGAGGGCTTT CGAGGCTGCG AACTTGCCAG CACTGGTGCT GAAGATCATG 
AGTGGCACCT TTGCACCTAT CTCTGACCGG TACAGCCCTG AGCTTCGCCA GCTGGTCCTG 
AGTCTACTCA GCCTGGAGCC TGCCCAGCGG CCACCACTCA GCCACATCAT GGCACAGCCC 
CTCTGCATCC GTGCCCTCCT CAACCTCCAC ACCGACGTGG GCAGTGTCCG CATGCGGAGG 
GCAGAGAAGT CCGTGGCCCC CAGCAGCACA GGGAGCAGGA CCACCAGTGT CCGCTGCAGA 
GGTATCCCCC GGGGACCTGT GAGGCCAGCC ATCCCACCAC CACTGTCGTC AGTGTATGCC 
TGGGGTGGTG GGCTGGGCAC CCCCCTGCGG CTGCCAATGC TCAACACAGA GGTGGTCCAG 
GTGGCAGCTG GGCGCACGCA GAAAGCCGGC GTCACGCGCT CTGGGCGTCT CATCCTGTGG 
GAGGCCCCAC CCCTAGGTGC AGGCGGAGGC AGTCTCCTCC CTGGGGCAGT GGAGCAGCCA 
CAGCCCCAGT TCATCTCGCG TTTCCTGGAG GGCCAGTCGG GCGTGACCAT CAAGCACGTG 
GCCTGTGGGG ACTTCTTCAC TGCCTGCCTG ACTGACAGAG GCATCATCAT GACATTCGGC 
AGCGGCAGCA ATGGGTGCCT AGGCCATGGC AGCCTCACTG ACATCAGCCA GCCCACCATT 
GTGGAGGCTT TGCTGGGCTA TGAAATGGTG CAGGTGGCCT GTGGGGCCTC TCACGTGCTG 
GCCCTGTCCA CTGAGCGAGA ACTATTTGCC TGGGGCCGTG GAGACAGCGG CAGACTGGGG 
CTAGGCACCA GGGAGTCCCA CAGCTGCCCC CAGCAGGTGC CCATGCCCCC AGGACAGGAA 
GCTCAGCGAG TTGTATGTGG CATCGATTCC TCCATGATCC TCACTGTGCC TGGCCAAGCC 
CTAGCCTGTG GGAGCAACAG GTTCAACAAG CTGGGCCTGG ACCACCTCTC CCTGGGGGAG 
GAGCCTGTCC CCCACCAGCA AGTGGAGGAG GCCCTGAGCT TCACACTACT AGGCTCTGCA 
CCCCTGGACC AGGAGCCTCT GCTGAGTATA GACCTGGGCA CTGCTCACTC AGCTGCTGTG 
ACTGCCTCGG GTGATTGCTA CACTTTTGGC AGCAATCAGC ACGGACAGTT GGGCACCAAT 
ACTCGCCGAG GCAGTCGGGC ACCCTGTAAG GTCCAAGGCC TTGAGGGCAT CAAGATGGCA 
ATGGTAGCCT GTGGGGATGC CTTCACTGTA GCTATTGGGG CAGAGGGCGA AGTGTACTCT 
TGGGGCAAAG GGGCGCGAGG TCGATTGGGA AGGAGGGATG AGGATGCCGG ACTCCCTCGG 
CCAGTGCAGT TGGATGAGAC ACACCCTTAC ACGGTGACTT CCGTGTCCTG TTGCCATGGA 
AACACCCTCC TGGCTGTTCG ATCGGTCACA GATGAGCCGG TCCCCCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016787650.1
CDS	35..2185
Translation	MSTEGRLPSCSACVKGELRVLTSAALTSRDGPRPCHVLFRIVHLCLRKADQKLVIIKQIPVEQMTKEERQAAQNECQVLKLLNHPNVIEYYENFLEDKALMIAMEYAPGGTLAEFIQKRCNSLLEEETILHFFVQILLALHHVHTHLILHRDLKTQNILLDKHRMVVKIGDFGISKILSSKSKAYTVVGTPCYISPELCEGKPYNQKSDIWALGCVLYELASLKRAFEAANLPALVLKIMSGTFAPISDRYSPELRQLVLSLLSLEPAQRPPLSHIMAQPLCIRALLNLHTDVGSVRMRRAEKSVAPSSTGSRTTSVRCRGIPRGPVRPAIPPPLSSVYAWGGGLGTPLRLPMLNTEVVQVAAGRTQKAGVTRSGRLILWEAPPLGAGGGSLLPGAVEQPQPQFISRFLEGQSGVTIKHVACGDFFTACLTDRGIIMTFGSGSNGCLGHGSLTDISQPTIVEALLGYEMVQVACGASHVLALSTERELFAWGRGDSGRLGLGTRESHSCPQQVPMPPGQEAQRVVCGIDSSMILTVPGQALACGSNRFNKLGLDHLSLGEEPVPHQQVEEALSFTLLGSAPLDQEPLLSIDLGTAHSAAVTASGDCYTFGSNQHGQLGTNTRRGSRAPCKVQGLEGIKMAMVACGDAFTVAIGAEGEVYSWGKGARGRLGRRDEDAGLPRPVQLDETHPYTVTSVSCCHGNTLLAVRSVTDEPVPP

Target ORF information:

RefSeq Version	XM_016932161.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes NIMA related kinase 8 (NEK8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016932161.1

ORF Insert Sequence:

ATGAGCACTG AGGGCAGATT ACCCTCCTGC AGCGCGTGTG TGAAAGGGGA GTTGAGAGTG 
CTGACGAGCG CGGCGCTCAC TAGTCGGGAC GGCCCGAGAC CGTGTCATGT CCTCTTCAGG 
ATTGTGCACC TGTGCCTGCG AAAGGCTGAC CAGAAGCTGG TGATCATCAA GCAGATTCCA 
GTGGAACAGA TGACCAAGGA AGAGCGGCAG GCAGCCCAGA ATGAGTGCCA GGTCCTCAAG 
CTGCTCAACC ACCCCAATGT CATTGAGTAC TACGAGAACT TCCTGGAAGA CAAAGCCCTT 
ATGATCGCCA TGGAATATGC ACCAGGCGGC ACTCTGGCTG AGTTCATCCA AAAGCGCTGT 
AATTCCCTGC TGGAGGAGGA GACCATCCTG CACTTCTTCG TGCAGATCCT GCTTGCACTG 
CATCATGTGC ACACCCACCT CATCCTGCAC CGAGACCTCA AGACCCAGAA CATCCTGCTT 
GACAAACACC GCATGGTCGT CAAGATCGGT GATTTCGGCA TCTCCAAGAT CCTTAGCAGC 
AAGAGCAAGG CCTACACGGT GGTGGGTACC CCATGCTATA TCTCCCCTGA GCTGTGTGAG 
GGCAAGCCCT ACAACCAGAA GAGTGACATC TGGGCCCTGG GCTGTGTCCT CTACGAGCTG 
GCCAGCCTCA AGAGGGCTTT CGAGGCTGCG AACTTGCCAG CACTGGTGCT GAAGATCATG 
AGTGGCACCT TTGCACCTAT CTCTGACCGG TACAGCCCTG AGCTTCGCCA GCTGGTCCTG 
AGTCTACTCA GCCTGGAGCC TGCCCAGCGG CCACCACTCA GCCACATCAT GGCACAGCCC 
CTCTGCATCC GTGCCCTCCT CAACCTCCAC ACCGACGTGG GCAGTGTCCG CATGCGGAGG 
GCAGAGAAGT CCGTGGCCCC CAGCAGCACA GGGAGCAGGA CCACCAGTGT CCGCTGCAGA 
GGTATCCCCC GGGGACCTGT GAGGCCAGCC ATCCCACCAC CACTGTCGTC AGTGTATGCC 
TGGGGTGGTG GGCTGGGCAC CCCCCTGCGG CTGCCAATGC TCAACACAGA GGTGGTCCAG 
GTGGCAGCTG GGCGCACGCA GAAAGCCGGC GTCACGCGCT CTGGGCGTCT CATCCTGTGG 
GAGGCCCCAC CCCTAGGTGC AGGCGGAGGC AGTCTCCTCC CTGGGGCAGT GGAGCAGCCA 
CAGCCCCAGT TCATCTCGCG TTTCCTGGAG GGCCAGTCGG GCGTGACCAT CAAGCACGTG 
GCCTGTGGGG ACTTCTTCAC TGCCTGCCTG ACTGACAGAG GCATCATCAT GACATTCGGC 
AGCGGCAGCA ATGGGTGCCT AGGCCATGGC AGCCTCACTG ACATCAGCCA GCCCACCATT 
GTGGAGGCTT TGCTGGGCTA TGAAATGGTG CAGGTGGCCT GTGGGGCCTC TCACGTGCTG 
GCCCTGTCCA CTGAGCGAGA ACTATTTGCC TGGGGCCGTG GAGACAGCGG CAGACTGGGG 
CTAGGCACCA GGGAGTCCCA CAGCTGCCCC CAGCAGGTGC CCATGCCCCC AGGACAGGAA 
GCTCAGCGAG TTGTATGTGG CATCGATTCC TCCATGATCC TCACTGTGCC TGGCCAAGCC 
CTAGCCTGTG GGAGCAACAG GTTCAACAAG CTGGGCCTGG ACCACCTCTC CCTGGGGGAG 
GAGCCTGTCC CCCACCAGCA AGTGGAGGAG GCCCTGAGCT TCACACTACT AGGCTCTGCA 
CCCCTGGACC AGGAGCCTCT GCTGAGTATA GACCTGGGCA CTGCTCACTC AGCTGCTGTG 
ACTGCCTCGG GTGATTGCTA CACTTTTGGC AGCAATCAGC ACGGACAGTT GGGCACCAAT 
ACTCGCCGAG GCAGTCGGGC ACCCTGTAAG GTCCAAGGCC TTGAGGGCAT CAAGATGGCA 
ATGGTAGCCT GTGGGGATGC CTTCACTGTA GCTATTGGGG CAGAGGGCGA AGTGTACTCT 
TGGGGCAAAG GGGCGCGAGG TCGATTGGGA AGGAGGGATG AGGATGCCGG ACTCCCTCGG 
CCAGTGCAGT TGGATGAGAC ACACCCTTAC ACGGTGACTT CCGTGTCCTG TTGCCATGGA 
AACACCCTCC TGGCTGTTCG ATCGGTCACA GATGAGCCGG TCCCCCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK8 ( XM_016932163.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016787652.1 Pan troglodytes NIMA related kinase 8 (NEK8), transcript variant X3, mRNA.

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CloneID	OPc72785
Clone ID Related Accession (Same CDS sequence)	XM_016932163.2 , XM_016932163.1
Accession Version	XM_016932163.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	serine/threonine-protein kinase Nek8 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036896.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016932163.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCAAGG AAGAGCGGCA GGCAGCCCAG AATGAGTGCC AGGTCCTCAA GCTGCTCAAC 
CACCCCAATG TCATTGAGTA CTACGAGAAC TTCCTGGAAG ACAAAGCCCT TATGATCGCC 
ATGGAATATG CACCAGGCGG CACTCTGGCT GAGTTCATCC AAAAGCGCTG TAATTCCCTG 
CTGGAGGAGG AGACCATCCT GCACTTCTTC GTGCAGATCC TGCTTGCACT GCATCATGTG 
CACACCCACC TCATCCTGCA CCGAGACCTC AAGACCCAGA ACATCCTGCT TGACAAACAC 
CGCATGGTCG TCAAGATCGG TGATTTCGGC ATCTCCAAGA TCCTTAGCAG CAAGAGCAAG 
GCCTACACGG TGGTGGGTAC CCCATGCTAT ATCTCCCCTG AGCTGTGTGA GGGCAAGCCC 
TACAACCAGA AGAGTGACAT CTGGGCCCTG GGCTGTGTCC TCTACGAGCT GGCCAGCCTC 
AAGAGGGCTT TCGAGGCTGC GAACTTGCCA GCACTGGTGC TGAAGATCAT GAGTGGCACC 
TTTGCACCTA TCTCTGACCG GTACAGCCCT GAGCTTCGCC AGCTGGTCCT GAGTCTACTC 
AGCCTGGAGC CTGCCCAGCG GCCACCACTC AGCCACATCA TGGCACAGCC CCTCTGCATC 
CGTGCCCTCC TCAACCTCCA CACCGACGTG GGCAGTGTCC GCATGCGGAG GGCAGAGAAG 
TCCGTGGCCC CCAGCAGCAC AGGGAGCAGG ACCACCAGTG TCCGCTGCAG AGGTATCCCC 
CGGGGACCTG TGAGGCCAGC CATCCCACCA CCACTGTCGT CAGTGTATGC CTGGGGTGGT 
GGGCTGGGCA CCCCCCTGCG GCTGCCAATG CTCAACACAG AGGTGGTCCA GGTGGCAGCT 
GGGCGCACGC AGAAAGCCGG CGTCACGCGC TCTGGGCGTC TCATCCTGTG GGAGGCCCCA 
CCCCTAGGTG CAGGCGGAGG CAGTCTCCTC CCTGGGGCAG TGGAGCAGCC ACAGCCCCAG 
TTCATCTCGC GTTTCCTGGA GGGCCAGTCG GGCGTGACCA TCAAGCACGT GGCCTGTGGG 
GACTTCTTCA CTGCCTGCCT GACTGACAGA GGCATCATCA TGACATTCGG CAGCGGCAGC 
AATGGGTGCC TAGGCCATGG CAGCCTCACT GACATCAGCC AGCCCACCAT TGTGGAGGCT 
TTGCTGGGCT ATGAAATGGT GCAGGTGGCC TGTGGGGCCT CTCACGTGCT GGCCCTGTCC 
ACTGAGCGAG AACTATTTGC CTGGGGCCGT GGAGACAGCG GCAGACTGGG GCTAGGCACC 
AGGGAGTCCC ACAGCTGCCC CCAGCAGGTG CCCATGCCCC CAGGACAGGA AGCTCAGCGA 
GTTGTATGTG GCATCGATTC CTCCATGATC CTCACTGTGC CTGGCCAAGC CCTAGCCTGT 
GGGAGCAACA GGTTCAACAA GCTGGGCCTG GACCACCTCT CCCTGGGGGA GGAGCCTGTC 
CCCCACCAGC AAGTGGAGGA GGCCCTGAGC TTCACACTAC TAGGCTCTGC ACCCCTGGAC 
CAGGAGCCTC TGCTGAGTAT AGACCTGGGC ACTGCTCACT CAGCTGCTGT GACTGCCTCG 
GGTGATTGCT ACACTTTTGG CAGCAATCAG CACGGACAGT TGGGCACCAA TACTCGCCGA 
GGCAGTCGGG CACCCTGTAA GGTCCAAGGC CTTGAGGGCA TCAAGATGGC AATGGTAGCC 
TGTGGGGATG CCTTCACTGT AGCTATTGGG GCAGAGGGCG AAGTGTACTC TTGGGGCAAA 
GGGGCGCGAG GTCGATTGGG AAGGAGGGAT GAGGATGCCG GACTCCCTCG GCCAGTGCAG 
TTGGATGAGA CACACCCTTA CACGGTGACT TCCGTGTCCT GTTGCCATGG AAACACCCTC 
CTGGCTGTTC GATCGGTCAC AGATGAGCCG GTCCCCCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016787652.1
CDS	317..2278
Translation	MTKEERQAAQNECQVLKLLNHPNVIEYYENFLEDKALMIAMEYAPGGTLAEFIQKRCNSLLEEETILHFFVQILLALHHVHTHLILHRDLKTQNILLDKHRMVVKIGDFGISKILSSKSKAYTVVGTPCYISPELCEGKPYNQKSDIWALGCVLYELASLKRAFEAANLPALVLKIMSGTFAPISDRYSPELRQLVLSLLSLEPAQRPPLSHIMAQPLCIRALLNLHTDVGSVRMRRAEKSVAPSSTGSRTTSVRCRGIPRGPVRPAIPPPLSSVYAWGGGLGTPLRLPMLNTEVVQVAAGRTQKAGVTRSGRLILWEAPPLGAGGGSLLPGAVEQPQPQFISRFLEGQSGVTIKHVACGDFFTACLTDRGIIMTFGSGSNGCLGHGSLTDISQPTIVEALLGYEMVQVACGASHVLALSTERELFAWGRGDSGRLGLGTRESHSCPQQVPMPPGQEAQRVVCGIDSSMILTVPGQALACGSNRFNKLGLDHLSLGEEPVPHQQVEEALSFTLLGSAPLDQEPLLSIDLGTAHSAAVTASGDCYTFGSNQHGQLGTNTRRGSRAPCKVQGLEGIKMAMVACGDAFTVAIGAEGEVYSWGKGARGRLGRRDEDAGLPRPVQLDETHPYTVTSVSCCHGNTLLAVRSVTDEPVPP

Target ORF information:

RefSeq Version	XM_016932163.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes NIMA related kinase 8 (NEK8), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016932163.2

ORF Insert Sequence:

ATGACCAAGG AAGAGCGGCA GGCAGCCCAG AATGAGTGCC AGGTCCTCAA GCTGCTCAAC 
CACCCCAATG TCATTGAGTA CTACGAGAAC TTCCTGGAAG ACAAAGCCCT TATGATCGCC 
ATGGAATATG CACCAGGCGG CACTCTGGCT GAGTTCATCC AAAAGCGCTG TAATTCCCTG 
CTGGAGGAGG AGACCATCCT GCACTTCTTC GTGCAGATCC TGCTTGCACT GCATCATGTG 
CACACCCACC TCATCCTGCA CCGAGACCTC AAGACCCAGA ACATCCTGCT TGACAAACAC 
CGCATGGTCG TCAAGATCGG TGATTTCGGC ATCTCCAAGA TCCTTAGCAG CAAGAGCAAG 
GCCTACACGG TGGTGGGTAC CCCATGCTAT ATCTCCCCTG AGCTGTGTGA GGGCAAGCCC 
TACAACCAGA AGAGTGACAT CTGGGCCCTG GGCTGTGTCC TCTACGAGCT GGCCAGCCTC 
AAGAGGGCTT TCGAGGCTGC GAACTTGCCA GCACTGGTGC TGAAGATCAT GAGTGGCACC 
TTTGCACCTA TCTCTGACCG GTACAGCCCT GAGCTTCGCC AGCTGGTCCT GAGTCTACTC 
AGCCTGGAGC CTGCCCAGCG GCCACCACTC AGCCACATCA TGGCACAGCC CCTCTGCATC 
CGTGCCCTCC TCAACCTCCA CACCGACGTG GGCAGTGTCC GCATGCGGAG GGCAGAGAAG 
TCCGTGGCCC CCAGCAGCAC AGGGAGCAGG ACCACCAGTG TCCGCTGCAG AGGTATCCCC 
CGGGGACCTG TGAGGCCAGC CATCCCACCA CCACTGTCGT CAGTGTATGC CTGGGGTGGT 
GGGCTGGGCA CCCCCCTGCG GCTGCCAATG CTCAACACAG AGGTGGTCCA GGTGGCAGCT 
GGGCGCACGC AGAAAGCCGG CGTCACGCGC TCTGGGCGTC TCATCCTGTG GGAGGCCCCA 
CCCCTAGGTG CAGGCGGAGG CAGTCTCCTC CCTGGGGCAG TGGAGCAGCC ACAGCCCCAG 
TTCATCTCGC GTTTCCTGGA GGGCCAGTCG GGCGTGACCA TCAAGCACGT GGCCTGTGGG 
GACTTCTTCA CTGCCTGCCT GACTGACAGA GGCATCATCA TGACATTCGG CAGCGGCAGC 
AATGGGTGCC TAGGCCATGG CAGCCTCACT GACATCAGCC AGCCCACCAT TGTGGAGGCT 
TTGCTGGGCT ATGAAATGGT GCAGGTGGCC TGTGGGGCCT CTCACGTGCT GGCCCTGTCC 
ACTGAGCGAG AACTATTTGC CTGGGGCCGT GGAGACAGCG GCAGACTGGG GCTAGGCACC 
AGGGAGTCCC ACAGCTGCCC CCAGCAGGTG CCCATGCCCC CAGGACAGGA AGCTCAGCGA 
GTTGTATGTG GCATCGATTC CTCCATGATC CTCACTGTGC CTGGCCAAGC CCTAGCCTGT 
GGGAGCAACA GGTTCAACAA GCTGGGCCTG GACCACCTCT CCCTGGGGGA GGAGCCTGTC 
CCCCACCAGC AAGTGGAGGA GGCCCTGAGC TTCACACTAC TAGGCTCTGC ACCCCTGGAC 
CAGGAGCCTC TGCTGAGTAT AGACCTGGGC ACTGCTCACT CAGCTGCTGT GACTGCCTCG 
GGTGATTGCT ACACTTTTGG CAGCAATCAG CACGGACAGT TGGGCACCAA TACTCGCCGA 
GGCAGTCGGG CACCCTGTAA GGTCCAAGGC CTTGAGGGCA TCAAGATGGC AATGGTAGCC 
TGTGGGGATG CCTTCACTGT AGCTATTGGG GCAGAGGGCG AAGTGTACTC TTGGGGCAAA 
GGGGCGCGAG GTCGATTGGG AAGGAGGGAT GAGGATGCCG GACTCCCTCG GCCAGTGCAG 
TTGGATGAGA CACACCCTTA CACGGTGACT TCCGTGTCCT GTTGCCATGG AAACACCCTC 
CTGGCTGTTC GATCGGTCAC AGATGAGCCG GTCCCCCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK8 ( XM_016932163.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016787652.1 Pan troglodytes NIMA related kinase 8 (NEK8), transcript variant X3, mRNA.

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CloneID	OPc72785
Clone ID Related Accession (Same CDS sequence)	XM_016932163.2 , XM_016932163.1
Accession Version	XM_016932163.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	serine/threonine-protein kinase Nek8 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006484.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCAAGG AAGAGCGGCA GGCAGCCCAG AATGAGTGCC AGGTCCTCAA GCTGCTCAAC 
CACCCCAATG TCATTGAGTA CTACGAGAAC TTCCTGGAAG ACAAAGCCCT TATGATCGCC 
ATGGAATATG CACCAGGCGG CACTCTGGCT GAGTTCATCC AAAAGCGCTG TAATTCCCTG 
CTGGAGGAGG AGACCATCCT GCACTTCTTC GTGCAGATCC TGCTTGCACT GCATCATGTG 
CACACCCACC TCATCCTGCA CCGAGACCTC AAGACCCAGA ACATCCTGCT TGACAAACAC 
CGCATGGTCG TCAAGATCGG TGATTTCGGC ATCTCCAAGA TCCTTAGCAG CAAGAGCAAG 
GCCTACACGG TGGTGGGTAC CCCATGCTAT ATCTCCCCTG AGCTGTGTGA GGGCAAGCCC 
TACAACCAGA AGAGTGACAT CTGGGCCCTG GGCTGTGTCC TCTACGAGCT GGCCAGCCTC 
AAGAGGGCTT TCGAGGCTGC GAACTTGCCA GCACTGGTGC TGAAGATCAT GAGTGGCACC 
TTTGCACCTA TCTCTGACCG GTACAGCCCT GAGCTTCGCC AGCTGGTCCT GAGTCTACTC 
AGCCTGGAGC CTGCCCAGCG GCCACCACTC AGCCACATCA TGGCACAGCC CCTCTGCATC 
CGTGCCCTCC TCAACCTCCA CACCGACGTG GGCAGTGTCC GCATGCGGAG GGCAGAGAAG 
TCCGTGGCCC CCAGCAGCAC AGGGAGCAGG ACCACCAGTG TCCGCTGCAG AGGTATCCCC 
CGGGGACCTG TGAGGCCAGC CATCCCACCA CCACTGTCGT CAGTGTATGC CTGGGGTGGT 
GGGCTGGGCA CCCCCCTGCG GCTGCCAATG CTCAACACAG AGGTGGTCCA GGTGGCAGCT 
GGGCGCACGC AGAAAGCCGG CGTCACGCGC TCTGGGCGTC TCATCCTGTG GGAGGCCCCA 
CCCCTAGGTG CAGGCGGAGG CAGTCTCCTC CCTGGGGCAG TGGAGCAGCC ACAGCCCCAG 
TTCATCTCGC GTTTCCTGGA GGGCCAGTCG GGCGTGACCA TCAAGCACGT GGCCTGTGGG 
GACTTCTTCA CTGCCTGCCT GACTGACAGA GGCATCATCA TGACATTCGG CAGCGGCAGC 
AATGGGTGCC TAGGCCATGG CAGCCTCACT GACATCAGCC AGCCCACCAT TGTGGAGGCT 
TTGCTGGGCT ATGAAATGGT GCAGGTGGCC TGTGGGGCCT CTCACGTGCT GGCCCTGTCC 
ACTGAGCGAG AACTATTTGC CTGGGGCCGT GGAGACAGCG GCAGACTGGG GCTAGGCACC 
AGGGAGTCCC ACAGCTGCCC CCAGCAGGTG CCCATGCCCC CAGGACAGGA AGCTCAGCGA 
GTTGTATGTG GCATCGATTC CTCCATGATC CTCACTGTGC CTGGCCAAGC CCTAGCCTGT 
GGGAGCAACA GGTTCAACAA GCTGGGCCTG GACCACCTCT CCCTGGGGGA GGAGCCTGTC 
CCCCACCAGC AAGTGGAGGA GGCCCTGAGC TTCACACTAC TAGGCTCTGC ACCCCTGGAC 
CAGGAGCCTC TGCTGAGTAT AGACCTGGGC ACTGCTCACT CAGCTGCTGT GACTGCCTCG 
GGTGATTGCT ACACTTTTGG CAGCAATCAG CACGGACAGT TGGGCACCAA TACTCGCCGA 
GGCAGTCGGG CACCCTGTAA GGTCCAAGGC CTTGAGGGCA TCAAGATGGC AATGGTAGCC 
TGTGGGGATG CCTTCACTGT AGCTATTGGG GCAGAGGGCG AAGTGTACTC TTGGGGCAAA 
GGGGCGCGAG GTCGATTGGG AAGGAGGGAT GAGGATGCCG GACTCCCTCG GCCAGTGCAG 
TTGGATGAGA CACACCCTTA CACGGTGACT TCCGTGTCCT GTTGCCATGG AAACACCCTC 
CTGGCTGTTC GATCGGTCAC AGATGAGCCG GTCCCCCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016787652.1
CDS	318..2279
Translation	MTKEERQAAQNECQVLKLLNHPNVIEYYENFLEDKALMIAMEYAPGGTLAEFIQKRCNSLLEEETILHFFVQILLALHHVHTHLILHRDLKTQNILLDKHRMVVKIGDFGISKILSSKSKAYTVVGTPCYISPELCEGKPYNQKSDIWALGCVLYELASLKRAFEAANLPALVLKIMSGTFAPISDRYSPELRQLVLSLLSLEPAQRPPLSHIMAQPLCIRALLNLHTDVGSVRMRRAEKSVAPSSTGSRTTSVRCRGIPRGPVRPAIPPPLSSVYAWGGGLGTPLRLPMLNTEVVQVAAGRTQKAGVTRSGRLILWEAPPLGAGGGSLLPGAVEQPQPQFISRFLEGQSGVTIKHVACGDFFTACLTDRGIIMTFGSGSNGCLGHGSLTDISQPTIVEALLGYEMVQVACGASHVLALSTERELFAWGRGDSGRLGLGTRESHSCPQQVPMPPGQEAQRVVCGIDSSMILTVPGQALACGSNRFNKLGLDHLSLGEEPVPHQQVEEALSFTLLGSAPLDQEPLLSIDLGTAHSAAVTASGDCYTFGSNQHGQLGTNTRRGSRAPCKVQGLEGIKMAMVACGDAFTVAIGAEGEVYSWGKGARGRLGRRDEDAGLPRPVQLDETHPYTVTSVSCCHGNTLLAVRSVTDEPVPP

Target ORF information:

RefSeq Version	XM_016932163.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes NIMA related kinase 8 (NEK8), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016932163.1

ORF Insert Sequence:

ATGACCAAGG AAGAGCGGCA GGCAGCCCAG AATGAGTGCC AGGTCCTCAA GCTGCTCAAC 
CACCCCAATG TCATTGAGTA CTACGAGAAC TTCCTGGAAG ACAAAGCCCT TATGATCGCC 
ATGGAATATG CACCAGGCGG CACTCTGGCT GAGTTCATCC AAAAGCGCTG TAATTCCCTG 
CTGGAGGAGG AGACCATCCT GCACTTCTTC GTGCAGATCC TGCTTGCACT GCATCATGTG 
CACACCCACC TCATCCTGCA CCGAGACCTC AAGACCCAGA ACATCCTGCT TGACAAACAC 
CGCATGGTCG TCAAGATCGG TGATTTCGGC ATCTCCAAGA TCCTTAGCAG CAAGAGCAAG 
GCCTACACGG TGGTGGGTAC CCCATGCTAT ATCTCCCCTG AGCTGTGTGA GGGCAAGCCC 
TACAACCAGA AGAGTGACAT CTGGGCCCTG GGCTGTGTCC TCTACGAGCT GGCCAGCCTC 
AAGAGGGCTT TCGAGGCTGC GAACTTGCCA GCACTGGTGC TGAAGATCAT GAGTGGCACC 
TTTGCACCTA TCTCTGACCG GTACAGCCCT GAGCTTCGCC AGCTGGTCCT GAGTCTACTC 
AGCCTGGAGC CTGCCCAGCG GCCACCACTC AGCCACATCA TGGCACAGCC CCTCTGCATC 
CGTGCCCTCC TCAACCTCCA CACCGACGTG GGCAGTGTCC GCATGCGGAG GGCAGAGAAG 
TCCGTGGCCC CCAGCAGCAC AGGGAGCAGG ACCACCAGTG TCCGCTGCAG AGGTATCCCC 
CGGGGACCTG TGAGGCCAGC CATCCCACCA CCACTGTCGT CAGTGTATGC CTGGGGTGGT 
GGGCTGGGCA CCCCCCTGCG GCTGCCAATG CTCAACACAG AGGTGGTCCA GGTGGCAGCT 
GGGCGCACGC AGAAAGCCGG CGTCACGCGC TCTGGGCGTC TCATCCTGTG GGAGGCCCCA 
CCCCTAGGTG CAGGCGGAGG CAGTCTCCTC CCTGGGGCAG TGGAGCAGCC ACAGCCCCAG 
TTCATCTCGC GTTTCCTGGA GGGCCAGTCG GGCGTGACCA TCAAGCACGT GGCCTGTGGG 
GACTTCTTCA CTGCCTGCCT GACTGACAGA GGCATCATCA TGACATTCGG CAGCGGCAGC 
AATGGGTGCC TAGGCCATGG CAGCCTCACT GACATCAGCC AGCCCACCAT TGTGGAGGCT 
TTGCTGGGCT ATGAAATGGT GCAGGTGGCC TGTGGGGCCT CTCACGTGCT GGCCCTGTCC 
ACTGAGCGAG AACTATTTGC CTGGGGCCGT GGAGACAGCG GCAGACTGGG GCTAGGCACC 
AGGGAGTCCC ACAGCTGCCC CCAGCAGGTG CCCATGCCCC CAGGACAGGA AGCTCAGCGA 
GTTGTATGTG GCATCGATTC CTCCATGATC CTCACTGTGC CTGGCCAAGC CCTAGCCTGT 
GGGAGCAACA GGTTCAACAA GCTGGGCCTG GACCACCTCT CCCTGGGGGA GGAGCCTGTC 
CCCCACCAGC AAGTGGAGGA GGCCCTGAGC TTCACACTAC TAGGCTCTGC ACCCCTGGAC 
CAGGAGCCTC TGCTGAGTAT AGACCTGGGC ACTGCTCACT CAGCTGCTGT GACTGCCTCG 
GGTGATTGCT ACACTTTTGG CAGCAATCAG CACGGACAGT TGGGCACCAA TACTCGCCGA 
GGCAGTCGGG CACCCTGTAA GGTCCAAGGC CTTGAGGGCA TCAAGATGGC AATGGTAGCC 
TGTGGGGATG CCTTCACTGT AGCTATTGGG GCAGAGGGCG AAGTGTACTC TTGGGGCAAA 
GGGGCGCGAG GTCGATTGGG AAGGAGGGAT GAGGATGCCG GACTCCCTCG GCCAGTGCAG 
TTGGATGAGA CACACCCTTA CACGGTGACT TCCGTGTCCT GTTGCCATGG AAACACCCTC 
CTGGCTGTTC GATCGGTCAC AGATGAGCCG GTCCCCCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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NEK8 cDNA ORF clone, Pan troglodytes(chimpanzee)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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