Nek8 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nek8
Entrez Gene ID	140859
Full Name	NIMA (never in mitosis gene a)-related expressed kinase 8
Synonyms	4632401F23Rik,b2b1449Clo,jck
General protein information	Preferred Names NIMA (never in mitosis gene a)-related expressed kinase 8 Names serine/threonine-protein kinase Nek8 NIMA-related expressed kinase 8 juvenile cystic kidney never in mitosis A-related kinase 8 nimA-related protein kinase 8
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11 B5\|11 46.74 cM
Summary	This gene encodes a NIMA-related kinase. Members of this serine/threonine protein kinase family are structurally-related to NIMA (never in mitosis, gene A) which controls mitotic signaling in Aspergillus nidulans. [provided by RefSeq, Jul 2008].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006532185.4	XP_006532248.1	serine/threonine-protein kinase Nek8 isoform X3
XM_006532183.3	XP_006532246.1	serine/threonine-protein kinase Nek8 isoform X1
XM_006532185.3	XP_006532248.1	serine/threonine-protein kinase Nek8 isoform X3
XM_006532183.4	XP_006532246.1	serine/threonine-protein kinase Nek8 isoform X1
XM_006532184.4	XP_006532247.1	serine/threonine-protein kinase Nek8 isoform X2
XM_006532184.3	XP_006532247.1	serine/threonine-protein kinase Nek8 isoform X2
NM_080849.3	NP_543125.1	serine/threonine-protein kinase Nek8

Pathways from BioSystems

Homologies

	NEK8	XP_001106986.2
Drosophila melanogaster (fruit fly)	niki	NP_651293.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006872	XP_308885.4
Canis lupus familiaris (dog)	NEK8	XP_548291.3
Pan troglodytes (chimpanzee)	NEK8	XP_511801.3
Mus musculus (house mouse)	Nek8	NP_543125.1
Homo sapiens (human)	NEK8	NP_835464.1
Rattus norvegicus (Norway rat)	Nek8	NP_001099274.1
Danio rerio (zebrafish)	nek8	NP_620776.1
Bos taurus (cattle)	NEK8	NP_001179652.1
Gallus gallus (chicken)	NEK8	XP_415822.3
Caenorhabditis elegans (roundworm)	nekl-1	NP_490968.2
Xenopus tropicalis (tropical clawed frog)	nek8	NP_001006906.1

Gene Ontology
Bibliography

Related articles in PubMed

Distinct oxylipin alterations in diverse models of cystic kidney diseases.
Monirujjaman M, Devassy JG, Yamaguchi T, Sidhu N, Kugita M, Gabbs M, Nagao S, Zhou J, Ravandi A, Aukema HM
Biochimica et biophysica acta1862(12)1562-1574(2017 12)

NEK8 regulates DNA damage-induced RAD51 foci formation and replication fork protection.
Abeyta A, Castella M, Jacquemont C, Taniguchi T
Cell cycle (Georgetown, Tex.)16(4)335-347(2017 Feb)

CaMKII as a pathological mediator of ER stress, oxidative stress, and mitochondrial dysfunction in a murine model of nephronophthisis.
Bracken C, Beauverger P, Duclos O, Russo RJ, Rogers KA, Husson H, Natoli TA, Ledbetter SR, Janiak P, Ibraghimov-Beskrovnaya O, Bukanov NO
American journal of physiology. Renal physiology310(11)F1414-22(2016 Jun)

High-resolution genetic localization of a modifying locus affecting disease severity in the juvenile cystic kidneys (jck) mouse model of polycystic kidney disease.
Beier DR
Mammalian genome : official journal of the International Mammalian Genome Society27(5-6)191-9(2016 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

NEK8 plays a critical role in replication fork stability through its regulation of the DNA repair and replication fork protection protein RAD51.
Title: NEK8 regulates DNA damage-induced RAD51 foci formation and replication fork protection.

We constructed sub-congenic lines to more specifically localize the modifier and have determined it resides in a 3.2 Mb interval containing 28 genes. These include Invs and Anks6, which are both excellent candidates for the modifier as mutations in these genes result in PKD and both genes are known to genetically and physically interact with Nek8.
Title: High-resolution genetic localization of a modifying locus affecting disease severity in the juvenile cystic kidneys (jck) mouse model of polycystic kidney disease.

NEK8-depleted inner medullary collecting duct cells exhibited a defective response to fluid shear.
Title: Loss of the ciliary kinase Nek8 causes left-right asymmetry defects.

Nek8 acts downstream of Inv function
Title: The ciliary protein Nek8/Nphp9 acts downstream of Inv/Nphp2 during pronephros morphogenesis and left-right establishment in zebrafish.

Inv acts as a molecular anchor for Nphp3 and Nek8 in the proximal segment of primary cilia.
Title: Inv acts as a molecular anchor for Nphp3 and Nek8 in the proximal segment of primary cilia.

The following Nek8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nek8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu18912	NM_080849.3 Latest version!	Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 8 (Nek8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$412.30 $589.00
OMu45547	XM_006532183.4 Latest version!	Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 8 (Nek8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu45548	XM_006532184.4 Latest version!	Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 8 (Nek8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu45549	XM_006532185.4 Latest version!	Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 8 (Nek8), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu45549	XM_006532185.3	Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 8 (Nek8), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu45547	XM_006532183.3	Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 8 (Nek8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu45548	XM_006532184.3	Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 8 (Nek8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
Hide Old Accession Versions >>

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Reference Sequences (Refseq)

Nek8 ( XM_006532185.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532248.1 Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 8 (Nek8), transcript variant X3, mRNA.

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CloneID	OMu45549
Clone ID Related Accession (Same CDS sequence)	XM_006532185.4 , XM_006532185.3
Accession Version	XM_006532185.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1977bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase Nek8 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006532185.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGT ACGAGCGGAT CCGAGTGGTG GGAAGAGGTG CCTTCGGGAT TGTGCACCTG 
TGCCTGCGAA AGGCCGACCA GAAGCTGGTG ATCCTCAAGC AGATCCCAGT GGAACAGATG 
ACCAAAGAAG AGAGGCAGGC GGCCCAGAAC GAGTGCCAGG TGCTCAAGCT GCTCAACCAC 
CCCAACGTCA TCGAGTACTA TGAGAATTTC CTAGAGGACA AGGCCCTCAT GATTGCTATG 
GAGTACGCAC CAGGTGGCAC CTTGGCCGAG TTCATCCAGA AGCGCTGCAA CTCTCTGCTA 
GAGGAGGAGA CGATCCTTCA CTTCTTTGTG CAGATCCTGC TGGCTCTGCA TCACGTGCAC 
ACACATCTCA TCCTGCATCG GGACCTCAAG ACACAGAACA TCCTTCTTGA CAAACACCGC 
ATGGTGGTCA AGATCGGTGA CTTTGGCATC TCTAAGATCC TCAGCAGCAA GAGCAAGGCC 
TACACGAACT TGCCAGCCCT GGTGCTGAAG ATCATGAGTG GCACCTTCGC ACCCATCTCT 
GACCGGTACA GCCCTGAGCT TCGCCAGCTC GTCCTGAGTC TGCTTAGCCT GGAACCTGCA 
CAGAGGCCAC CCCTCAGCCA CATCATGGCG CAGCCCCTCT GCATCCGGGC CCTACTCAAC 
ATCCACACCG ATGTGGGCAG CGTGCGCATG CGGAGGGCAG AGAAGTCCCT GACCCCAGGA 
CCACCTATAG CCTCTGGCAG CACAGGGAGC AGGGCCACCA GTGCCCGATG TAGGGGTGTC 
CCCCGGGGAC CTGTGAGACC GGCTATCCCA CCGCCACTGT CTTCGGTCTA CGCTTGGGGT 
GGTGGCCTTA GCAGTCCCCT GAGGTTGCCA ATGCTCAACA CGGAGGTGGT CCAGGTAGCC 
GCTGGGCGCA CGCAGAAGGC TGGCGTTACG CGCTCTGGAC GCCTCATCCT GTGGGAGGCC 
CCACCCCTGG GAGCTGGAGG CGGCACACTC CTTCCTGGAG CCGTGGAGCT GCCTCAGCCC 
CAGTTTGTCT CTCGTTTCCT GGAAGGCCAG TCAGGTGTGA CCATCAAGCA TGTGGCCTGC 
GGGGACCTGT TCACCGCTTG CTTGACAGAC CGAGGCATTA TCATGACGTT CGGCAGTGGC 
AGCAATGGGT GTTTAGGTCA CGGCAACCTG ACTGACATCA GCCAGCCCAC CATTGTAGAA 
GCCTTGCTGG GCTATGAGAT GGTGCAGGTG GCCTGTGGGG CCTCTCATGT GCTGGCCCTG 
TCCACAGATG GAGAGTTATT CGCCTGGGGC AGAGGAGATG GTGGTAGACT GGGACTAGGC 
ACCAGGGAGT CCCATAACTG CCCTCAGCAG GTGCCTGTGG CCCCAGGACA GGAAGCTCAG 
AGAGTTGTCT GTGGTATTGA CTCTTCCATG ATCCTCACCT CTCCAGGCAG GGTCCTGGCC 
TGTGGAAGTA ACAGGTTCAA CAAGCTAGGC TTGGACCACC TATCCCTGGA CGAGGAGCCT 
GTTCCCTACC AGCAAGTGGA GGAGGCCCTG AGCTTCACAC CACTGGGCTC TGCACCTCTG 
GACCAGGAGC CCCTGCTGTG TGTGGACCTT GGCACTGCTC ATTCAGCTGC TATCACTGCT 
TCTGGGGACT GCTATACTTT TGGCAGCAAT CAACATGGAC AGCTGGGCAC CAGTTCCCGC 
AGGGTCAGCA GGGCACCCTG TCGGGTCCAA GGCCTGGAGG GCATCAAGAT GGTGATGGTG 
GCCTGCGGGG ATGCCTTCAC TGTGGCCGTT GGGGCAGAGG GGGAAGTGTA TTCTTGGGGC 
AAAGGAACCC GAGGCCGACT GGGAAGGAGG GATGAGGATG CGGGGCTGCC TAGGCCAGTG 
CAGCTGGATG AGACTCATCC TTACATGGTG ACTTCAGTGT CCTGCTGCCA TGGGAACACT 
CTGCTGGCTG TTCGATGTGA GTTTTCTTCT CCTATCTCAC CCACATCACT AGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532248.1
CDS	94..2070
Translation	MEKYERIRVVGRGAFGIVHLCLRKADQKLVILKQIPVEQMTKEERQAAQNECQVLKLLNHPNVIEYYENFLEDKALMIAMEYAPGGTLAEFIQKRCNSLLEEETILHFFVQILLALHHVHTHLILHRDLKTQNILLDKHRMVVKIGDFGISKILSSKSKAYTNLPALVLKIMSGTFAPISDRYSPELRQLVLSLLSLEPAQRPPLSHIMAQPLCIRALLNIHTDVGSVRMRRAEKSLTPGPPIASGSTGSRATSARCRGVPRGPVRPAIPPPLSSVYAWGGGLSSPLRLPMLNTEVVQVAAGRTQKAGVTRSGRLILWEAPPLGAGGGTLLPGAVELPQPQFVSRFLEGQSGVTIKHVACGDLFTACLTDRGIIMTFGSGSNGCLGHGNLTDISQPTIVEALLGYEMVQVACGASHVLALSTDGELFAWGRGDGGRLGLGTRESHNCPQQVPVAPGQEAQRVVCGIDSSMILTSPGRVLACGSNRFNKLGLDHLSLDEEPVPYQQVEEALSFTPLGSAPLDQEPLLCVDLGTAHSAAITASGDCYTFGSNQHGQLGTSSRRVSRAPCRVQGLEGIKMVMVACGDAFTVAVGAEGEVYSWGKGTRGRLGRRDEDAGLPRPVQLDETHPYMVTSVSCCHGNTLLAVRCEFSSPISPTSLG

Target ORF information:

RefSeq Version	XM_006532185.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 8 (Nek8), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532185.3

ORF Insert Sequence:

ATGGAGAAGT ACGAGCGGAT CCGAGTGGTG GGAAGAGGTG CCTTCGGGAT TGTGCACCTG 
TGCCTGCGAA AGGCCGACCA GAAGCTGGTG ATCCTCAAGC AGATCCCAGT GGAACAGATG 
ACCAAAGAAG AGAGGCAGGC GGCCCAGAAC GAGTGCCAGG TGCTCAAGCT GCTCAACCAC 
CCCAACGTCA TCGAGTACTA TGAGAATTTC CTAGAGGACA AGGCCCTCAT GATTGCTATG 
GAGTACGCAC CAGGTGGCAC CTTGGCCGAG TTCATCCAGA AGCGCTGCAA CTCTCTGCTA 
GAGGAGGAGA CGATCCTTCA CTTCTTTGTG CAGATCCTGC TGGCTCTGCA TCACGTGCAC 
ACACATCTCA TCCTGCATCG GGACCTCAAG ACACAGAACA TCCTTCTTGA CAAACACCGC 
ATGGTGGTCA AGATCGGTGA CTTTGGCATC TCTAAGATCC TCAGCAGCAA GAGCAAGGCC 
TACACGAACT TGCCAGCCCT GGTGCTGAAG ATCATGAGTG GCACCTTCGC ACCCATCTCT 
GACCGGTACA GCCCTGAGCT TCGCCAGCTC GTCCTGAGTC TGCTTAGCCT GGAACCTGCA 
CAGAGGCCAC CCCTCAGCCA CATCATGGCG CAGCCCCTCT GCATCCGGGC CCTACTCAAC 
ATCCACACCG ATGTGGGCAG CGTGCGCATG CGGAGGGCAG AGAAGTCCCT GACCCCAGGA 
CCACCTATAG CCTCTGGCAG CACAGGGAGC AGGGCCACCA GTGCCCGATG TAGGGGTGTC 
CCCCGGGGAC CTGTGAGACC GGCTATCCCA CCGCCACTGT CTTCGGTCTA CGCTTGGGGT 
GGTGGCCTTA GCAGTCCCCT GAGGTTGCCA ATGCTCAACA CGGAGGTGGT CCAGGTAGCC 
GCTGGGCGCA CGCAGAAGGC TGGCGTTACG CGCTCTGGAC GCCTCATCCT GTGGGAGGCC 
CCACCCCTGG GAGCTGGAGG CGGCACACTC CTTCCTGGAG CCGTGGAGCT GCCTCAGCCC 
CAGTTTGTCT CTCGTTTCCT GGAAGGCCAG TCAGGTGTGA CCATCAAGCA TGTGGCCTGC 
GGGGACCTGT TCACCGCTTG CTTGACAGAC CGAGGCATTA TCATGACGTT CGGCAGTGGC 
AGCAATGGGT GTTTAGGTCA CGGCAACCTG ACTGACATCA GCCAGCCCAC CATTGTAGAA 
GCCTTGCTGG GCTATGAGAT GGTGCAGGTG GCCTGTGGGG CCTCTCATGT GCTGGCCCTG 
TCCACAGATG GAGAGTTATT CGCCTGGGGC AGAGGAGATG GTGGTAGACT GGGACTAGGC 
ACCAGGGAGT CCCATAACTG CCCTCAGCAG GTGCCTGTGG CCCCAGGACA GGAAGCTCAG 
AGAGTTGTCT GTGGTATTGA CTCTTCCATG ATCCTCACCT CTCCAGGCAG GGTCCTGGCC 
TGTGGAAGTA ACAGGTTCAA CAAGCTAGGC TTGGACCACC TATCCCTGGA CGAGGAGCCT 
GTTCCCTACC AGCAAGTGGA GGAGGCCCTG AGCTTCACAC CACTGGGCTC TGCACCTCTG 
GACCAGGAGC CCCTGCTGTG TGTGGACCTT GGCACTGCTC ATTCAGCTGC TATCACTGCT 
TCTGGGGACT GCTATACTTT TGGCAGCAAT CAACATGGAC AGCTGGGCAC CAGTTCCCGC 
AGGGTCAGCA GGGCACCCTG TCGGGTCCAA GGCCTGGAGG GCATCAAGAT GGTGATGGTG 
GCCTGCGGGG ATGCCTTCAC TGTGGCCGTT GGGGCAGAGG GGGAAGTGTA TTCTTGGGGC 
AAAGGAACCC GAGGCCGACT GGGAAGGAGG GATGAGGATG CGGGGCTGCC TAGGCCAGTG 
CAGCTGGATG AGACTCATCC TTACATGGTG ACTTCAGTGT CCTGCTGCCA TGGGAACACT 
CTGCTGGCTG TTCGATGTGA GTTTTCTTCT CCTATCTCAC CCACATCACT AGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nek8 ( NM_080849.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_543125.1 Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 8 (Nek8), mRNA.

Price & Availability↑

CloneID	OMu18912
Clone ID Related Accession (Same CDS sequence)	NM_080849.3
Accession Version	NM_080849.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-11
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase Nek8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BB646522.1, AF407579.1 and BC070457.1. On Jan 30, 2009 this sequence version replaced NM_080849.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC070457.1, AF407579.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGAAGT ACGAGCGGAT CCGAGTGGTG GGAAGAGGTG CCTTCGGGAT TGTGCACCTG 
TGCCTGCGAA AGGCCGACCA GAAGCTGGTG ATCCTCAAGC AGATCCCAGT GGAACAGATG 
ACCAAAGAAG AGAGGCAGGC GGCCCAGAAC GAGTGCCAGG TGCTCAAGCT GCTCAACCAC 
CCCAACGTCA TCGAGTACTA TGAGAATTTC CTAGAGGACA AGGCCCTCAT GATTGCTATG 
GAGTACGCAC CAGGTGGCAC CTTGGCCGAG TTCATCCAGA AGCGCTGCAA CTCTCTGCTA 
GAGGAGGAGA CGATCCTTCA CTTCTTTGTG CAGATCCTGC TGGCTCTGCA TCACGTGCAC 
ACACATCTCA TCCTGCATCG GGACCTCAAG ACACAGAACA TCCTTCTTGA CAAACACCGC 
ATGGTGGTCA AGATCGGTGA CTTTGGCATC TCTAAGATCC TCAGCAGCAA GAGCAAGGCC 
TACACGGTGG TGGGTACACC CTGCTACATC TCCCCTGAGC TGTGTGAGGG CAAACCCTAC 
AACCAGAAGA GTGACATCTG GGCTCTGGGC TGTGTCTTAT ATGAGCTGGC CAGCCTCAAG 
AGGGCCTTCG AGGCTGCAAA CTTGCCAGCC CTGGTGCTGA AGATCATGAG TGGCACCTTC 
GCACCCATCT CTGACCGGTA CAGCCCTGAG CTTCGCCAGC TCGTCCTGAG TCTGCTTAGC 
CTGGAACCTG CACAGAGGCC ACCCCTCAGC CACATCATGG CGCAGCCCCT CTGCATCCGG 
GCCCTACTCA ACATCCACAC CGATGTGGGC AGCGTGCGCA TGCGGAGGGC AGAGAAGTCC 
CTGACCCCAG GACCACCTAT AGCCTCTGGC AGCACAGGGA GCAGGGCCAC CAGTGCCCGA 
TGTAGGGGTG TCCCCCGGGG ACCTGTGAGA CCGGCTATCC CACCGCCACT GTCTTCGGTC 
TACGCTTGGG GTGGTGGCCT TAGCAGTCCC CTGAGGTTGC CAATGCTCAA CACGGAGGTG 
GTCCAGGTAG CCGCTGGGCG CACGCAGAAG GCTGGCGTTA CGCGCTCTGG ACGCCTCATC 
CTGTGGGAGG CCCCACCCCT GGGAGCTGGA GGCGGCACAC TCCTTCCTGG AGCCGTGGAG 
CTGCCTCAGC CCCAGTTTGT CTCTCGTTTC CTGGAAGGCC AGTCAGGTGT GACCATCAAG 
CATGTGGCCT GCGGGGACCT GTTCACCGCT TGCTTGACAG ACCGAGGCAT TATCATGACG 
TTCGGCAGTG GCAGCAATGG GTGTTTAGGT CACGGCAACC TGACTGACAT CAGCCAGCCC 
ACCATTGTAG AAGCCTTGCT GGGCTATGAG ATGGTGCAGG TGGCCTGTGG GGCCTCTCAT 
GTGCTGGCCC TGTCCACAGA TGGAGAGTTA TTCGCCTGGG GCAGAGGAGA TGGTGGTAGA 
CTGGGACTAG GCACCAGGGA GTCCCATAAC TGCCCTCAGC AGGTGCCTGT GGCCCCAGGA 
CAGGAAGCTC AGAGAGTTGT CTGTGGTATT GACTCTTCCA TGATCCTCAC CTCTCCAGGC 
AGGGTCCTGG CCTGTGGAAG TAACAGGTTC AACAAGCTAG GCTTGGACCA CCTATCCCTG 
GACGAGGAGC CTGTTCCCTA CCAGCAAGTG GAGGAGGCCC TGAGCTTCAC ACCACTGGGC 
TCTGCACCTC TGGACCAGGA GCCCCTGCTG TGTGTGGACC TTGGCACTGC TCATTCAGCT 
GCTATCACTG CTTCTGGGGA CTGCTATACT TTTGGCAGCA ATCAACATGG ACAGCTGGGC 
ACCAGTTCCC GCAGGGTCAG CAGGGCACCC TGTCGGGTCC AAGGCCTGGA GGGCATCAAG 
ATGGTGATGG TGGCCTGCGG GGATGCCTTC ACTGTGGCCG TTGGGGCAGA GGGGGAAGTG 
TATTCTTGGG GCAAAGGAAC CCGAGGCCGA CTGGGAAGGA GGGATGAGGA TGCGGGGCTG 
CCTAGGCCAG TGCAGCTGGA TGAGACTCAT CCTTACATGG TGACTTCAGT GTCCTGCTGC 
CATGGGAACA CTCTGCTGGC TGTTCGATCA GTCACAGATG AACCAGTTCC CCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_543125.1
CDS	61..2157
Translation	MEKYERIRVVGRGAFGIVHLCLRKADQKLVILKQIPVEQMTKEERQAAQNECQVLKLLNHPNVIEYYENFLEDKALMIAMEYAPGGTLAEFIQKRCNSLLEEETILHFFVQILLALHHVHTHLILHRDLKTQNILLDKHRMVVKIGDFGISKILSSKSKAYTVVGTPCYISPELCEGKPYNQKSDIWALGCVLYELASLKRAFEAANLPALVLKIMSGTFAPISDRYSPELRQLVLSLLSLEPAQRPPLSHIMAQPLCIRALLNIHTDVGSVRMRRAEKSLTPGPPIASGSTGSRATSARCRGVPRGPVRPAIPPPLSSVYAWGGGLSSPLRLPMLNTEVVQVAAGRTQKAGVTRSGRLILWEAPPLGAGGGTLLPGAVELPQPQFVSRFLEGQSGVTIKHVACGDLFTACLTDRGIIMTFGSGSNGCLGHGNLTDISQPTIVEALLGYEMVQVACGASHVLALSTDGELFAWGRGDGGRLGLGTRESHNCPQQVPVAPGQEAQRVVCGIDSSMILTSPGRVLACGSNRFNKLGLDHLSLDEEPVPYQQVEEALSFTPLGSAPLDQEPLLCVDLGTAHSAAITASGDCYTFGSNQHGQLGTSSRRVSRAPCRVQGLEGIKMVMVACGDAFTVAVGAEGEVYSWGKGTRGRLGRRDEDAGLPRPVQLDETHPYMVTSVSCCHGNTLLAVRSVTDEPVPP

Target ORF information:

RefSeq Version	NM_080849.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 8 (Nek8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080849.3

ORF Insert Sequence:

ATGGAGAAGT ACGAGCGGAT CCGAGTGGTG GGAAGAGGTG CCTTCGGGAT TGTGCACCTG 
TGCCTGCGAA AGGCCGACCA GAAGCTGGTG ATCCTCAAGC AGATCCCAGT GGAACAGATG 
ACCAAAGAAG AGAGGCAGGC GGCCCAGAAC GAGTGCCAGG TGCTCAAGCT GCTCAACCAC 
CCCAACGTCA TCGAGTACTA TGAGAATTTC CTAGAGGACA AGGCCCTCAT GATTGCTATG 
GAGTACGCAC CAGGTGGCAC CTTGGCCGAG TTCATCCAGA AGCGCTGCAA CTCTCTGCTA 
GAGGAGGAGA CGATCCTTCA CTTCTTTGTG CAGATCCTGC TGGCTCTGCA TCACGTGCAC 
ACACATCTCA TCCTGCATCG GGACCTCAAG ACACAGAACA TCCTTCTTGA CAAACACCGC 
ATGGTGGTCA AGATCGGTGA CTTTGGCATC TCTAAGATCC TCAGCAGCAA GAGCAAGGCC 
TACACGGTGG TGGGTACACC CTGCTACATC TCCCCTGAGC TGTGTGAGGG CAAACCCTAC 
AACCAGAAGA GTGACATCTG GGCTCTGGGC TGTGTCTTAT ATGAGCTGGC CAGCCTCAAG 
AGGGCCTTCG AGGCTGCAAA CTTGCCAGCC CTGGTGCTGA AGATCATGAG TGGCACCTTC 
GCACCCATCT CTGACCGGTA CAGCCCTGAG CTTCGCCAGC TCGTCCTGAG TCTGCTTAGC 
CTGGAACCTG CACAGAGGCC ACCCCTCAGC CACATCATGG CGCAGCCCCT CTGCATCCGG 
GCCCTACTCA ACATCCACAC CGATGTGGGC AGCGTGCGCA TGCGGAGGGC AGAGAAGTCC 
CTGACCCCAG GACCACCTAT AGCCTCTGGC AGCACAGGGA GCAGGGCCAC CAGTGCCCGA 
TGTAGGGGTG TCCCCCGGGG ACCTGTGAGA CCGGCTATCC CACCGCCACT GTCTTCGGTC 
TACGCTTGGG GTGGTGGCCT TAGCAGTCCC CTGAGGTTGC CAATGCTCAA CACGGAGGTG 
GTCCAGGTAG CCGCTGGGCG CACGCAGAAG GCTGGCGTTA CGCGCTCTGG ACGCCTCATC 
CTGTGGGAGG CCCCACCCCT GGGAGCTGGA GGCGGCACAC TCCTTCCTGG AGCCGTGGAG 
CTGCCTCAGC CCCAGTTTGT CTCTCGTTTC CTGGAAGGCC AGTCAGGTGT GACCATCAAG 
CATGTGGCCT GCGGGGACCT GTTCACCGCT TGCTTGACAG ACCGAGGCAT TATCATGACG 
TTCGGCAGTG GCAGCAATGG GTGTTTAGGT CACGGCAACC TGACTGACAT CAGCCAGCCC 
ACCATTGTAG AAGCCTTGCT GGGCTATGAG ATGGTGCAGG TGGCCTGTGG GGCCTCTCAT 
GTGCTGGCCC TGTCCACAGA TGGAGAGTTA TTCGCCTGGG GCAGAGGAGA TGGTGGTAGA 
CTGGGACTAG GCACCAGGGA GTCCCATAAC TGCCCTCAGC AGGTGCCTGT GGCCCCAGGA 
CAGGAAGCTC AGAGAGTTGT CTGTGGTATT GACTCTTCCA TGATCCTCAC CTCTCCAGGC 
AGGGTCCTGG CCTGTGGAAG TAACAGGTTC AACAAGCTAG GCTTGGACCA CCTATCCCTG 
GACGAGGAGC CTGTTCCCTA CCAGCAAGTG GAGGAGGCCC TGAGCTTCAC ACCACTGGGC 
TCTGCACCTC TGGACCAGGA GCCCCTGCTG TGTGTGGACC TTGGCACTGC TCATTCAGCT 
GCTATCACTG CTTCTGGGGA CTGCTATACT TTTGGCAGCA ATCAACATGG ACAGCTGGGC 
ACCAGTTCCC GCAGGGTCAG CAGGGCACCC TGTCGGGTCC AAGGCCTGGA GGGCATCAAG 
ATGGTGATGG TGGCCTGCGG GGATGCCTTC ACTGTGGCCG TTGGGGCAGA GGGGGAAGTG 
TATTCTTGGG GCAAAGGAAC CCGAGGCCGA CTGGGAAGGA GGGATGAGGA TGCGGGGCTG 
CCTAGGCCAG TGCAGCTGGA TGAGACTCAT CCTTACATGG TGACTTCAGT GTCCTGCTGC 
CATGGGAACA CTCTGCTGGC TGTTCGATCA GTCACAGATG AACCAGTTCC CCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nek8 ( XM_006532183.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532246.1 Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 8 (Nek8), transcript variant X1, mRNA.

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CloneID	OMu45547
Clone ID Related Accession (Same CDS sequence)	XM_006532183.3 , XM_006532183.4
Accession Version	XM_006532183.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2109bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase Nek8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006532183.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGT ACGAGCGGAT CCGAGTGGTG GGAAGAGGTG CCTTCGGGAT TGTGCACCTG 
TGCCTGCGAA AGGCCGACCA GAAGCTGGTG ATCCTCAAGC AGATCCCAGT GGAACAGATG 
ACCAAAGAAG AGAGGCAGGC GGCCCAGAAC GAGTGCCAGG TGCTCAAGCT GCTCAACCAC 
CCCAACGTCA TCGAGTACTA TGAGAATTTC CTAGAGGACA AGGCCCTCAT GATTGCTATG 
GAGTACGCAC CAGGTGGCAC CTTGGCCGAG TTCATCCAGA AGCGCTGCAA CTCTCTGCTA 
GAGGAGGAGA CGATCCTTCA CTTCTTTGTG CAGATCCTGC TGGCTCTGCA TCACGTGCAC 
ACACATCTCA TCCTGCATCG GGACCTCAAG ACACAGAACA TCCTTCTTGA CAAACACCGC 
ATGGTGGTCA AGATCGGTGA CTTTGGCATC TCTAAGATCC TCAGCAGCAA GAGCAAGGCC 
TACACGGTGG TGGGTACACC CTGCTACATC TCCCCTGAGC TGTGTGAGGG CAAACCCTAC 
AACCAGAAGA GTGACATCTG GGCTCTGGGC TGTGTCTTAT ATGAGCTGGC CAGCCTCAAG 
AGGGCCTTCG AGGCTGCAAA CTTGCCAGCC CTGGTGCTGA AGATCATGAG TGGCACCTTC 
GCACCCATCT CTGACCGGTA CAGCCCTGAG CTTCGCCAGC TCGTCCTGAG TCTGCTTAGC 
CTGGAACCTG CACAGAGGCC ACCCCTCAGC CACATCATGG CGCAGCCCCT CTGCATCCGG 
GCCCTACTCA ACATCCACAC CGATGTGGGC AGCGTGCGCA TGCGGAGGGC AGAGAAGTCC 
CTGACCCCAG GACCACCTAT AGCCTCTGGC AGCACAGGGA GCAGGGCCAC CAGTGCCCGA 
TGTAGGGGTG TCCCCCGGGG ACCTGTGAGA CCGGCTATCC CACCGCCACT GTCTTCGGTC 
TACGCTTGGG GTGGTGGCCT TAGCAGTCCC CTGAGGTTGC CAATGCTCAA CACGGAGGTG 
GTCCAGGTAG CCGCTGGGCG CACGCAGAAG GCTGGCGTTA CGCGCTCTGG ACGCCTCATC 
CTGTGGGAGG CCCCACCCCT GGGAGCTGGA GGCGGCACAC TCCTTCCTGG AGCCGTGGAG 
CTGCCTCAGC CCCAGTTTGT CTCTCGTTTC CTGGAAGGCC AGTCAGGTGT GACCATCAAG 
CATGTGGCCT GCGGGGACCT GTTCACCGCT TGCTTGACAG ACCGAGGCAT TATCATGACG 
TTCGGCAGTG GCAGCAATGG GTGTTTAGGT CACGGCAACC TGACTGACAT CAGCCAGCCC 
ACCATTGTAG AAGCCTTGCT GGGCTATGAG ATGGTGCAGG TGGCCTGTGG GGCCTCTCAT 
GTGCTGGCCC TGTCCACAGA TGGAGAGTTA TTCGCCTGGG GCAGAGGAGA TGGTGGTAGA 
CTGGGACTAG GCACCAGGGA GTCCCATAAC TGCCCTCAGC AGGTGCCTGT GGCCCCAGGA 
CAGGAAGCTC AGAGAGTTGT CTGTGGTATT GACTCTTCCA TGATCCTCAC CTCTCCAGGC 
AGGGTCCTGG CCTGTGGAAG TAACAGGTTC AACAAGCTAG GCTTGGACCA CCTATCCCTG 
GACGAGGAGC CTGTTCCCTA CCAGCAAGTG GAGGAGGCCC TGAGCTTCAC ACCACTGGGC 
TCTGCACCTC TGGACCAGGA GCCCCTGCTG TGTGTGGACC TTGGCACTGC TCATTCAGCT 
GCTATCACTG CTTCTGGGGA CTGCTATACT TTTGGCAGCA ATCAACATGG ACAGCTGGGC 
ACCAGTTCCC GCAGGGTCAG CAGGGCACCC TGTCGGGTCC AAGGCCTGGA GGGCATCAAG 
ATGGTGATGG TGGCCTGCGG GGATGCCTTC ACTGTGGCCG TTGGGGCAGA GGGGGAAGTG 
TATTCTTGGG GCAAAGGAAC CCGAGGCCGA CTGGGAAGGA GGGATGAGGA TGCGGGGCTG 
CCTAGGCCAG TGCAGCTGGA TGAGACTCAT CCTTACATGG TGACTTCAGT GTCCTGCTGC 
CATGGGAACA CTCTGCTGGC TGTTCGATGT GAGTTTTCTT CTCCTATCTC ACCCACATCA 
CTAGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532246.1
CDS	94..2202
Translation	MEKYERIRVVGRGAFGIVHLCLRKADQKLVILKQIPVEQMTKEERQAAQNECQVLKLLNHPNVIEYYENFLEDKALMIAMEYAPGGTLAEFIQKRCNSLLEEETILHFFVQILLALHHVHTHLILHRDLKTQNILLDKHRMVVKIGDFGISKILSSKSKAYTVVGTPCYISPELCEGKPYNQKSDIWALGCVLYELASLKRAFEAANLPALVLKIMSGTFAPISDRYSPELRQLVLSLLSLEPAQRPPLSHIMAQPLCIRALLNIHTDVGSVRMRRAEKSLTPGPPIASGSTGSRATSARCRGVPRGPVRPAIPPPLSSVYAWGGGLSSPLRLPMLNTEVVQVAAGRTQKAGVTRSGRLILWEAPPLGAGGGTLLPGAVELPQPQFVSRFLEGQSGVTIKHVACGDLFTACLTDRGIIMTFGSGSNGCLGHGNLTDISQPTIVEALLGYEMVQVACGASHVLALSTDGELFAWGRGDGGRLGLGTRESHNCPQQVPVAPGQEAQRVVCGIDSSMILTSPGRVLACGSNRFNKLGLDHLSLDEEPVPYQQVEEALSFTPLGSAPLDQEPLLCVDLGTAHSAAITASGDCYTFGSNQHGQLGTSSRRVSRAPCRVQGLEGIKMVMVACGDAFTVAVGAEGEVYSWGKGTRGRLGRRDEDAGLPRPVQLDETHPYMVTSVSCCHGNTLLAVRCEFSSPISPTSLG

Target ORF information:

RefSeq Version	XM_006532183.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 8 (Nek8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532183.3

ORF Insert Sequence:

ATGGAGAAGT ACGAGCGGAT CCGAGTGGTG GGAAGAGGTG CCTTCGGGAT TGTGCACCTG 
TGCCTGCGAA AGGCCGACCA GAAGCTGGTG ATCCTCAAGC AGATCCCAGT GGAACAGATG 
ACCAAAGAAG AGAGGCAGGC GGCCCAGAAC GAGTGCCAGG TGCTCAAGCT GCTCAACCAC 
CCCAACGTCA TCGAGTACTA TGAGAATTTC CTAGAGGACA AGGCCCTCAT GATTGCTATG 
GAGTACGCAC CAGGTGGCAC CTTGGCCGAG TTCATCCAGA AGCGCTGCAA CTCTCTGCTA 
GAGGAGGAGA CGATCCTTCA CTTCTTTGTG CAGATCCTGC TGGCTCTGCA TCACGTGCAC 
ACACATCTCA TCCTGCATCG GGACCTCAAG ACACAGAACA TCCTTCTTGA CAAACACCGC 
ATGGTGGTCA AGATCGGTGA CTTTGGCATC TCTAAGATCC TCAGCAGCAA GAGCAAGGCC 
TACACGGTGG TGGGTACACC CTGCTACATC TCCCCTGAGC TGTGTGAGGG CAAACCCTAC 
AACCAGAAGA GTGACATCTG GGCTCTGGGC TGTGTCTTAT ATGAGCTGGC CAGCCTCAAG 
AGGGCCTTCG AGGCTGCAAA CTTGCCAGCC CTGGTGCTGA AGATCATGAG TGGCACCTTC 
GCACCCATCT CTGACCGGTA CAGCCCTGAG CTTCGCCAGC TCGTCCTGAG TCTGCTTAGC 
CTGGAACCTG CACAGAGGCC ACCCCTCAGC CACATCATGG CGCAGCCCCT CTGCATCCGG 
GCCCTACTCA ACATCCACAC CGATGTGGGC AGCGTGCGCA TGCGGAGGGC AGAGAAGTCC 
CTGACCCCAG GACCACCTAT AGCCTCTGGC AGCACAGGGA GCAGGGCCAC CAGTGCCCGA 
TGTAGGGGTG TCCCCCGGGG ACCTGTGAGA CCGGCTATCC CACCGCCACT GTCTTCGGTC 
TACGCTTGGG GTGGTGGCCT TAGCAGTCCC CTGAGGTTGC CAATGCTCAA CACGGAGGTG 
GTCCAGGTAG CCGCTGGGCG CACGCAGAAG GCTGGCGTTA CGCGCTCTGG ACGCCTCATC 
CTGTGGGAGG CCCCACCCCT GGGAGCTGGA GGCGGCACAC TCCTTCCTGG AGCCGTGGAG 
CTGCCTCAGC CCCAGTTTGT CTCTCGTTTC CTGGAAGGCC AGTCAGGTGT GACCATCAAG 
CATGTGGCCT GCGGGGACCT GTTCACCGCT TGCTTGACAG ACCGAGGCAT TATCATGACG 
TTCGGCAGTG GCAGCAATGG GTGTTTAGGT CACGGCAACC TGACTGACAT CAGCCAGCCC 
ACCATTGTAG AAGCCTTGCT GGGCTATGAG ATGGTGCAGG TGGCCTGTGG GGCCTCTCAT 
GTGCTGGCCC TGTCCACAGA TGGAGAGTTA TTCGCCTGGG GCAGAGGAGA TGGTGGTAGA 
CTGGGACTAG GCACCAGGGA GTCCCATAAC TGCCCTCAGC AGGTGCCTGT GGCCCCAGGA 
CAGGAAGCTC AGAGAGTTGT CTGTGGTATT GACTCTTCCA TGATCCTCAC CTCTCCAGGC 
AGGGTCCTGG CCTGTGGAAG TAACAGGTTC AACAAGCTAG GCTTGGACCA CCTATCCCTG 
GACGAGGAGC CTGTTCCCTA CCAGCAAGTG GAGGAGGCCC TGAGCTTCAC ACCACTGGGC 
TCTGCACCTC TGGACCAGGA GCCCCTGCTG TGTGTGGACC TTGGCACTGC TCATTCAGCT 
GCTATCACTG CTTCTGGGGA CTGCTATACT TTTGGCAGCA ATCAACATGG ACAGCTGGGC 
ACCAGTTCCC GCAGGGTCAG CAGGGCACCC TGTCGGGTCC AAGGCCTGGA GGGCATCAAG 
ATGGTGATGG TGGCCTGCGG GGATGCCTTC ACTGTGGCCG TTGGGGCAGA GGGGGAAGTG 
TATTCTTGGG GCAAAGGAAC CCGAGGCCGA CTGGGAAGGA GGGATGAGGA TGCGGGGCTG 
CCTAGGCCAG TGCAGCTGGA TGAGACTCAT CCTTACATGG TGACTTCAGT GTCCTGCTGC 
CATGGGAACA CTCTGCTGGC TGTTCGATGT GAGTTTTCTT CTCCTATCTC ACCCACATCA 
CTAGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nek8 ( XM_006532184.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532247.1 Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 8 (Nek8), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu45548
Clone ID Related Accession (Same CDS sequence)	XM_006532184.4 , XM_006532184.3
Accession Version	XM_006532184.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2094bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase Nek8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006532184.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGT ACGAGCGGAT CCGAGTGGTG GGAAGAGGTG CCTTCGGGAT TGTGCACCTG 
TGCCTGCGAA AGGCCGACCA GAAGCTGGTG ATCCTCAAGC AGATCCCAGT GGAACAGATG 
ACCAAAGAAG AGAGGCAGGC GGCCCAGAAC GAGTGCCAGG TGCTCAAGCT GCTCAACCAC 
CCCAACGTCA TCGAGTACTA TGAGAATTTC CTAGAGGACA AGGCCCTCAT GATTGCTATG 
GAGTACGCAC CAGGTGGCAC CTTGGCCGAG TTCATCCAGA AGCGCTGCAA CTCTCTGCTA 
GAGGAGGAGA CGATCCTTCA CTTCTTTGTG CAGATCCTGC TGGCTCTGCA TCACGTGCAC 
ACACATCTCA TCCTGCATCG GGACCTCAAG ACACAGAACA TCCTTCTTGA CAAACACCGC 
ATGGTGGTCA AGATCGGTGA CTTTGGCATC TCTAAGATCC TCAGCAGCAA GAGCAAGGCC 
TACACGGTGG TGGGTACACC CTGCTACATC TCCCCTGAGC TGTGTGAGGG CAAACCCTAC 
AACCAGAAGA GTGACATCTG GGCTCTGGGC TGTGTCTTAT ATGAGCTGGC CAGCCTCAAG 
AGGGCCTTCG AGGCTGCAAA CTTGCCAGCC CTGGTGCTGA AGATCATGAG TGGCACCTTC 
GCACCCATCT CTGACCGGTA CAGCCCTGAG CTTCGCCAGC TCGTCCTGAG TCTGCTTAGC 
CTGGAACCTG CACAGAGGCC ACCCCTCAGC CACATCATGG CGCAGCCCCT CTGCATCCGG 
GCCCTACTCA ACATCCACAC CGATGTGGGC AGCGTGCGCA TGCGGAGGGC AGAGAAGTCC 
CTGACCCCAG GACCACCTAT AGCCTCTGGC AGCACAGGGA GCAGGGCCAC CAGTGCCCGA 
TGTAGGGGTG TCCCCCGGGG ACCTGTGAGA CCGGCTATCC CACCGCCACT GTCTTCGGTC 
TACGCTTGGG GTGGTGGCCT TAGCAGTCCC CTGAGGTTGC CAATGCTCAA CACGGAGGTG 
GTCCAGGTAG CCGCTGGGCG CACGCAGAAG GCTGGCGTTA CGCGCTCTGG ACGCCTCATC 
CTGTGGGAGG CCCCACCCCT GGGAGCTGGA GGCGGCACAC TCCTTCCTGG AGCCGTGGAG 
CTGCCTCAGC CCCAGTTTGT CTCTCGTTTC CTGGAAGGCC AGTCAGGTGT GACCATCAAG 
CATGTGGCCT GCGGGGACCT GTTCACCGCT TGCTTGACAG ACCGAGGCAT TATCATGACG 
TTCGGCAGTG GCAGCAATGG GTGTTTAGGT CACGGCAACC TGACTGACAT CAGCCAGCCC 
ACCATTGTAG AAGCCTTGCT GGGCTATGAG ATGGTGCAGG TGGCCTGTGG GGCCTCTCAT 
GTGCTGGCCC TGTCCACAGA TGGAGAGTTA TTCGCCTGGG GCAGAGGAGA TGGTGGTAGA 
CTGGGACTAG GCACCAGGGA GTCCCATAAC TGCCCTCAGC AGGTGCCTGT GGCCCCAGGA 
CAGGAAGCTC AGAGAGTTGT CTGTGGTATT GACTCTTCCA TGATCCTCAC CTCTCCAGGC 
AGGGTCCTGG CCTGTGGAAG TAACAGGTTC AACAAGCTAG GCTTGGACCA CCTATCCCTG 
GACGAGGAGC CTGTTCCCTA CCAGCAAGTG GAGGAGGCCC TGAGCTTCAC ACCACTGGGC 
TCTGCACCTC TGGACCAGGA GCCCCTGCTG TGTGTGGACC TTGGCACTGC TCATTCAGCT 
GCTATCACTG CTTCTGGGGA CTGCTATACT TTTGGCAGCA ATCAACATGG ACAGCTGGGC 
ACCAGTTCCC GCAGGGTCAG CAGGGCACCC TGTCGGGTCC AAGGCCTGGA GGGCATCAAG 
ATGGTGATGG TGGCCTGCGG GGATGCCTTC ACTGTGGCCG TTGGGGCAGA GGGGGAAGTG 
TATTCTTGGG GCAAAGGAAC CCGAGGCCGA CTGGGAAGGA GGGATGAGGA TGCGGGGCTG 
CCTAGGCCAG TGCAGCTGGA TGAGACTCAT CCTTACATGG TGACTTCAGT GTCCTGCTGC 
CATGGGAACA CTCTGCTGGC TGTTCGATTC ACAGATGAAC CAGTTCCCCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532247.1
CDS	94..2187
Translation	MEKYERIRVVGRGAFGIVHLCLRKADQKLVILKQIPVEQMTKEERQAAQNECQVLKLLNHPNVIEYYENFLEDKALMIAMEYAPGGTLAEFIQKRCNSLLEEETILHFFVQILLALHHVHTHLILHRDLKTQNILLDKHRMVVKIGDFGISKILSSKSKAYTVVGTPCYISPELCEGKPYNQKSDIWALGCVLYELASLKRAFEAANLPALVLKIMSGTFAPISDRYSPELRQLVLSLLSLEPAQRPPLSHIMAQPLCIRALLNIHTDVGSVRMRRAEKSLTPGPPIASGSTGSRATSARCRGVPRGPVRPAIPPPLSSVYAWGGGLSSPLRLPMLNTEVVQVAAGRTQKAGVTRSGRLILWEAPPLGAGGGTLLPGAVELPQPQFVSRFLEGQSGVTIKHVACGDLFTACLTDRGIIMTFGSGSNGCLGHGNLTDISQPTIVEALLGYEMVQVACGASHVLALSTDGELFAWGRGDGGRLGLGTRESHNCPQQVPVAPGQEAQRVVCGIDSSMILTSPGRVLACGSNRFNKLGLDHLSLDEEPVPYQQVEEALSFTPLGSAPLDQEPLLCVDLGTAHSAAITASGDCYTFGSNQHGQLGTSSRRVSRAPCRVQGLEGIKMVMVACGDAFTVAVGAEGEVYSWGKGTRGRLGRRDEDAGLPRPVQLDETHPYMVTSVSCCHGNTLLAVRFTDEPVPP

Target ORF information:

RefSeq Version	XM_006532184.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 8 (Nek8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532184.3

ORF Insert Sequence:

ATGGAGAAGT ACGAGCGGAT CCGAGTGGTG GGAAGAGGTG CCTTCGGGAT TGTGCACCTG 
TGCCTGCGAA AGGCCGACCA GAAGCTGGTG ATCCTCAAGC AGATCCCAGT GGAACAGATG 
ACCAAAGAAG AGAGGCAGGC GGCCCAGAAC GAGTGCCAGG TGCTCAAGCT GCTCAACCAC 
CCCAACGTCA TCGAGTACTA TGAGAATTTC CTAGAGGACA AGGCCCTCAT GATTGCTATG 
GAGTACGCAC CAGGTGGCAC CTTGGCCGAG TTCATCCAGA AGCGCTGCAA CTCTCTGCTA 
GAGGAGGAGA CGATCCTTCA CTTCTTTGTG CAGATCCTGC TGGCTCTGCA TCACGTGCAC 
ACACATCTCA TCCTGCATCG GGACCTCAAG ACACAGAACA TCCTTCTTGA CAAACACCGC 
ATGGTGGTCA AGATCGGTGA CTTTGGCATC TCTAAGATCC TCAGCAGCAA GAGCAAGGCC 
TACACGGTGG TGGGTACACC CTGCTACATC TCCCCTGAGC TGTGTGAGGG CAAACCCTAC 
AACCAGAAGA GTGACATCTG GGCTCTGGGC TGTGTCTTAT ATGAGCTGGC CAGCCTCAAG 
AGGGCCTTCG AGGCTGCAAA CTTGCCAGCC CTGGTGCTGA AGATCATGAG TGGCACCTTC 
GCACCCATCT CTGACCGGTA CAGCCCTGAG CTTCGCCAGC TCGTCCTGAG TCTGCTTAGC 
CTGGAACCTG CACAGAGGCC ACCCCTCAGC CACATCATGG CGCAGCCCCT CTGCATCCGG 
GCCCTACTCA ACATCCACAC CGATGTGGGC AGCGTGCGCA TGCGGAGGGC AGAGAAGTCC 
CTGACCCCAG GACCACCTAT AGCCTCTGGC AGCACAGGGA GCAGGGCCAC CAGTGCCCGA 
TGTAGGGGTG TCCCCCGGGG ACCTGTGAGA CCGGCTATCC CACCGCCACT GTCTTCGGTC 
TACGCTTGGG GTGGTGGCCT TAGCAGTCCC CTGAGGTTGC CAATGCTCAA CACGGAGGTG 
GTCCAGGTAG CCGCTGGGCG CACGCAGAAG GCTGGCGTTA CGCGCTCTGG ACGCCTCATC 
CTGTGGGAGG CCCCACCCCT GGGAGCTGGA GGCGGCACAC TCCTTCCTGG AGCCGTGGAG 
CTGCCTCAGC CCCAGTTTGT CTCTCGTTTC CTGGAAGGCC AGTCAGGTGT GACCATCAAG 
CATGTGGCCT GCGGGGACCT GTTCACCGCT TGCTTGACAG ACCGAGGCAT TATCATGACG 
TTCGGCAGTG GCAGCAATGG GTGTTTAGGT CACGGCAACC TGACTGACAT CAGCCAGCCC 
ACCATTGTAG AAGCCTTGCT GGGCTATGAG ATGGTGCAGG TGGCCTGTGG GGCCTCTCAT 
GTGCTGGCCC TGTCCACAGA TGGAGAGTTA TTCGCCTGGG GCAGAGGAGA TGGTGGTAGA 
CTGGGACTAG GCACCAGGGA GTCCCATAAC TGCCCTCAGC AGGTGCCTGT GGCCCCAGGA 
CAGGAAGCTC AGAGAGTTGT CTGTGGTATT GACTCTTCCA TGATCCTCAC CTCTCCAGGC 
AGGGTCCTGG CCTGTGGAAG TAACAGGTTC AACAAGCTAG GCTTGGACCA CCTATCCCTG 
GACGAGGAGC CTGTTCCCTA CCAGCAAGTG GAGGAGGCCC TGAGCTTCAC ACCACTGGGC 
TCTGCACCTC TGGACCAGGA GCCCCTGCTG TGTGTGGACC TTGGCACTGC TCATTCAGCT 
GCTATCACTG CTTCTGGGGA CTGCTATACT TTTGGCAGCA ATCAACATGG ACAGCTGGGC 
ACCAGTTCCC GCAGGGTCAG CAGGGCACCC TGTCGGGTCC AAGGCCTGGA GGGCATCAAG 
ATGGTGATGG TGGCCTGCGG GGATGCCTTC ACTGTGGCCG TTGGGGCAGA GGGGGAAGTG 
TATTCTTGGG GCAAAGGAAC CCGAGGCCGA CTGGGAAGGA GGGATGAGGA TGCGGGGCTG 
CCTAGGCCAG TGCAGCTGGA TGAGACTCAT CCTTACATGG TGACTTCAGT GTCCTGCTGC 
CATGGGAACA CTCTGCTGGC TGTTCGATTC ACAGATGAAC CAGTTCCCCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nek8 ( XM_006532183.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532246.1 Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 8 (Nek8), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu45547
Clone ID Related Accession (Same CDS sequence)	XM_006532183.3 , XM_006532183.4
Accession Version	XM_006532183.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2109bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase Nek8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006532183.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGT ACGAGCGGAT CCGAGTGGTG GGAAGAGGTG CCTTCGGGAT TGTGCACCTG 
TGCCTGCGAA AGGCCGACCA GAAGCTGGTG ATCCTCAAGC AGATCCCAGT GGAACAGATG 
ACCAAAGAAG AGAGGCAGGC GGCCCAGAAC GAGTGCCAGG TGCTCAAGCT GCTCAACCAC 
CCCAACGTCA TCGAGTACTA TGAGAATTTC CTAGAGGACA AGGCCCTCAT GATTGCTATG 
GAGTACGCAC CAGGTGGCAC CTTGGCCGAG TTCATCCAGA AGCGCTGCAA CTCTCTGCTA 
GAGGAGGAGA CGATCCTTCA CTTCTTTGTG CAGATCCTGC TGGCTCTGCA TCACGTGCAC 
ACACATCTCA TCCTGCATCG GGACCTCAAG ACACAGAACA TCCTTCTTGA CAAACACCGC 
ATGGTGGTCA AGATCGGTGA CTTTGGCATC TCTAAGATCC TCAGCAGCAA GAGCAAGGCC 
TACACGGTGG TGGGTACACC CTGCTACATC TCCCCTGAGC TGTGTGAGGG CAAACCCTAC 
AACCAGAAGA GTGACATCTG GGCTCTGGGC TGTGTCTTAT ATGAGCTGGC CAGCCTCAAG 
AGGGCCTTCG AGGCTGCAAA CTTGCCAGCC CTGGTGCTGA AGATCATGAG TGGCACCTTC 
GCACCCATCT CTGACCGGTA CAGCCCTGAG CTTCGCCAGC TCGTCCTGAG TCTGCTTAGC 
CTGGAACCTG CACAGAGGCC ACCCCTCAGC CACATCATGG CGCAGCCCCT CTGCATCCGG 
GCCCTACTCA ACATCCACAC CGATGTGGGC AGCGTGCGCA TGCGGAGGGC AGAGAAGTCC 
CTGACCCCAG GACCACCTAT AGCCTCTGGC AGCACAGGGA GCAGGGCCAC CAGTGCCCGA 
TGTAGGGGTG TCCCCCGGGG ACCTGTGAGA CCGGCTATCC CACCGCCACT GTCTTCGGTC 
TACGCTTGGG GTGGTGGCCT TAGCAGTCCC CTGAGGTTGC CAATGCTCAA CACGGAGGTG 
GTCCAGGTAG CCGCTGGGCG CACGCAGAAG GCTGGCGTTA CGCGCTCTGG ACGCCTCATC 
CTGTGGGAGG CCCCACCCCT GGGAGCTGGA GGCGGCACAC TCCTTCCTGG AGCCGTGGAG 
CTGCCTCAGC CCCAGTTTGT CTCTCGTTTC CTGGAAGGCC AGTCAGGTGT GACCATCAAG 
CATGTGGCCT GCGGGGACCT GTTCACCGCT TGCTTGACAG ACCGAGGCAT TATCATGACG 
TTCGGCAGTG GCAGCAATGG GTGTTTAGGT CACGGCAACC TGACTGACAT CAGCCAGCCC 
ACCATTGTAG AAGCCTTGCT GGGCTATGAG ATGGTGCAGG TGGCCTGTGG GGCCTCTCAT 
GTGCTGGCCC TGTCCACAGA TGGAGAGTTA TTCGCCTGGG GCAGAGGAGA TGGTGGTAGA 
CTGGGACTAG GCACCAGGGA GTCCCATAAC TGCCCTCAGC AGGTGCCTGT GGCCCCAGGA 
CAGGAAGCTC AGAGAGTTGT CTGTGGTATT GACTCTTCCA TGATCCTCAC CTCTCCAGGC 
AGGGTCCTGG CCTGTGGAAG TAACAGGTTC AACAAGCTAG GCTTGGACCA CCTATCCCTG 
GACGAGGAGC CTGTTCCCTA CCAGCAAGTG GAGGAGGCCC TGAGCTTCAC ACCACTGGGC 
TCTGCACCTC TGGACCAGGA GCCCCTGCTG TGTGTGGACC TTGGCACTGC TCATTCAGCT 
GCTATCACTG CTTCTGGGGA CTGCTATACT TTTGGCAGCA ATCAACATGG ACAGCTGGGC 
ACCAGTTCCC GCAGGGTCAG CAGGGCACCC TGTCGGGTCC AAGGCCTGGA GGGCATCAAG 
ATGGTGATGG TGGCCTGCGG GGATGCCTTC ACTGTGGCCG TTGGGGCAGA GGGGGAAGTG 
TATTCTTGGG GCAAAGGAAC CCGAGGCCGA CTGGGAAGGA GGGATGAGGA TGCGGGGCTG 
CCTAGGCCAG TGCAGCTGGA TGAGACTCAT CCTTACATGG TGACTTCAGT GTCCTGCTGC 
CATGGGAACA CTCTGCTGGC TGTTCGATGT GAGTTTTCTT CTCCTATCTC ACCCACATCA 
CTAGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532246.1
CDS	90..2198
Translation	MEKYERIRVVGRGAFGIVHLCLRKADQKLVILKQIPVEQMTKEERQAAQNECQVLKLLNHPNVIEYYENFLEDKALMIAMEYAPGGTLAEFIQKRCNSLLEEETILHFFVQILLALHHVHTHLILHRDLKTQNILLDKHRMVVKIGDFGISKILSSKSKAYTVVGTPCYISPELCEGKPYNQKSDIWALGCVLYELASLKRAFEAANLPALVLKIMSGTFAPISDRYSPELRQLVLSLLSLEPAQRPPLSHIMAQPLCIRALLNIHTDVGSVRMRRAEKSLTPGPPIASGSTGSRATSARCRGVPRGPVRPAIPPPLSSVYAWGGGLSSPLRLPMLNTEVVQVAAGRTQKAGVTRSGRLILWEAPPLGAGGGTLLPGAVELPQPQFVSRFLEGQSGVTIKHVACGDLFTACLTDRGIIMTFGSGSNGCLGHGNLTDISQPTIVEALLGYEMVQVACGASHVLALSTDGELFAWGRGDGGRLGLGTRESHNCPQQVPVAPGQEAQRVVCGIDSSMILTSPGRVLACGSNRFNKLGLDHLSLDEEPVPYQQVEEALSFTPLGSAPLDQEPLLCVDLGTAHSAAITASGDCYTFGSNQHGQLGTSSRRVSRAPCRVQGLEGIKMVMVACGDAFTVAVGAEGEVYSWGKGTRGRLGRRDEDAGLPRPVQLDETHPYMVTSVSCCHGNTLLAVRCEFSSPISPTSLG

Target ORF information:

RefSeq Version	XM_006532183.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 8 (Nek8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532183.4

ORF Insert Sequence:

ATGGAGAAGT ACGAGCGGAT CCGAGTGGTG GGAAGAGGTG CCTTCGGGAT TGTGCACCTG 
TGCCTGCGAA AGGCCGACCA GAAGCTGGTG ATCCTCAAGC AGATCCCAGT GGAACAGATG 
ACCAAAGAAG AGAGGCAGGC GGCCCAGAAC GAGTGCCAGG TGCTCAAGCT GCTCAACCAC 
CCCAACGTCA TCGAGTACTA TGAGAATTTC CTAGAGGACA AGGCCCTCAT GATTGCTATG 
GAGTACGCAC CAGGTGGCAC CTTGGCCGAG TTCATCCAGA AGCGCTGCAA CTCTCTGCTA 
GAGGAGGAGA CGATCCTTCA CTTCTTTGTG CAGATCCTGC TGGCTCTGCA TCACGTGCAC 
ACACATCTCA TCCTGCATCG GGACCTCAAG ACACAGAACA TCCTTCTTGA CAAACACCGC 
ATGGTGGTCA AGATCGGTGA CTTTGGCATC TCTAAGATCC TCAGCAGCAA GAGCAAGGCC 
TACACGGTGG TGGGTACACC CTGCTACATC TCCCCTGAGC TGTGTGAGGG CAAACCCTAC 
AACCAGAAGA GTGACATCTG GGCTCTGGGC TGTGTCTTAT ATGAGCTGGC CAGCCTCAAG 
AGGGCCTTCG AGGCTGCAAA CTTGCCAGCC CTGGTGCTGA AGATCATGAG TGGCACCTTC 
GCACCCATCT CTGACCGGTA CAGCCCTGAG CTTCGCCAGC TCGTCCTGAG TCTGCTTAGC 
CTGGAACCTG CACAGAGGCC ACCCCTCAGC CACATCATGG CGCAGCCCCT CTGCATCCGG 
GCCCTACTCA ACATCCACAC CGATGTGGGC AGCGTGCGCA TGCGGAGGGC AGAGAAGTCC 
CTGACCCCAG GACCACCTAT AGCCTCTGGC AGCACAGGGA GCAGGGCCAC CAGTGCCCGA 
TGTAGGGGTG TCCCCCGGGG ACCTGTGAGA CCGGCTATCC CACCGCCACT GTCTTCGGTC 
TACGCTTGGG GTGGTGGCCT TAGCAGTCCC CTGAGGTTGC CAATGCTCAA CACGGAGGTG 
GTCCAGGTAG CCGCTGGGCG CACGCAGAAG GCTGGCGTTA CGCGCTCTGG ACGCCTCATC 
CTGTGGGAGG CCCCACCCCT GGGAGCTGGA GGCGGCACAC TCCTTCCTGG AGCCGTGGAG 
CTGCCTCAGC CCCAGTTTGT CTCTCGTTTC CTGGAAGGCC AGTCAGGTGT GACCATCAAG 
CATGTGGCCT GCGGGGACCT GTTCACCGCT TGCTTGACAG ACCGAGGCAT TATCATGACG 
TTCGGCAGTG GCAGCAATGG GTGTTTAGGT CACGGCAACC TGACTGACAT CAGCCAGCCC 
ACCATTGTAG AAGCCTTGCT GGGCTATGAG ATGGTGCAGG TGGCCTGTGG GGCCTCTCAT 
GTGCTGGCCC TGTCCACAGA TGGAGAGTTA TTCGCCTGGG GCAGAGGAGA TGGTGGTAGA 
CTGGGACTAG GCACCAGGGA GTCCCATAAC TGCCCTCAGC AGGTGCCTGT GGCCCCAGGA 
CAGGAAGCTC AGAGAGTTGT CTGTGGTATT GACTCTTCCA TGATCCTCAC CTCTCCAGGC 
AGGGTCCTGG CCTGTGGAAG TAACAGGTTC AACAAGCTAG GCTTGGACCA CCTATCCCTG 
GACGAGGAGC CTGTTCCCTA CCAGCAAGTG GAGGAGGCCC TGAGCTTCAC ACCACTGGGC 
TCTGCACCTC TGGACCAGGA GCCCCTGCTG TGTGTGGACC TTGGCACTGC TCATTCAGCT 
GCTATCACTG CTTCTGGGGA CTGCTATACT TTTGGCAGCA ATCAACATGG ACAGCTGGGC 
ACCAGTTCCC GCAGGGTCAG CAGGGCACCC TGTCGGGTCC AAGGCCTGGA GGGCATCAAG 
ATGGTGATGG TGGCCTGCGG GGATGCCTTC ACTGTGGCCG TTGGGGCAGA GGGGGAAGTG 
TATTCTTGGG GCAAAGGAAC CCGAGGCCGA CTGGGAAGGA GGGATGAGGA TGCGGGGCTG 
CCTAGGCCAG TGCAGCTGGA TGAGACTCAT CCTTACATGG TGACTTCAGT GTCCTGCTGC 
CATGGGAACA CTCTGCTGGC TGTTCGATGT GAGTTTTCTT CTCCTATCTC ACCCACATCA 
CTAGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nek8 ( XM_006532184.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532247.1 Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 8 (Nek8), transcript variant X2, mRNA.

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CloneID	OMu45548
Clone ID Related Accession (Same CDS sequence)	XM_006532184.4 , XM_006532184.3
Accession Version	XM_006532184.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2094bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase Nek8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006532184.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGT ACGAGCGGAT CCGAGTGGTG GGAAGAGGTG CCTTCGGGAT TGTGCACCTG 
TGCCTGCGAA AGGCCGACCA GAAGCTGGTG ATCCTCAAGC AGATCCCAGT GGAACAGATG 
ACCAAAGAAG AGAGGCAGGC GGCCCAGAAC GAGTGCCAGG TGCTCAAGCT GCTCAACCAC 
CCCAACGTCA TCGAGTACTA TGAGAATTTC CTAGAGGACA AGGCCCTCAT GATTGCTATG 
GAGTACGCAC CAGGTGGCAC CTTGGCCGAG TTCATCCAGA AGCGCTGCAA CTCTCTGCTA 
GAGGAGGAGA CGATCCTTCA CTTCTTTGTG CAGATCCTGC TGGCTCTGCA TCACGTGCAC 
ACACATCTCA TCCTGCATCG GGACCTCAAG ACACAGAACA TCCTTCTTGA CAAACACCGC 
ATGGTGGTCA AGATCGGTGA CTTTGGCATC TCTAAGATCC TCAGCAGCAA GAGCAAGGCC 
TACACGGTGG TGGGTACACC CTGCTACATC TCCCCTGAGC TGTGTGAGGG CAAACCCTAC 
AACCAGAAGA GTGACATCTG GGCTCTGGGC TGTGTCTTAT ATGAGCTGGC CAGCCTCAAG 
AGGGCCTTCG AGGCTGCAAA CTTGCCAGCC CTGGTGCTGA AGATCATGAG TGGCACCTTC 
GCACCCATCT CTGACCGGTA CAGCCCTGAG CTTCGCCAGC TCGTCCTGAG TCTGCTTAGC 
CTGGAACCTG CACAGAGGCC ACCCCTCAGC CACATCATGG CGCAGCCCCT CTGCATCCGG 
GCCCTACTCA ACATCCACAC CGATGTGGGC AGCGTGCGCA TGCGGAGGGC AGAGAAGTCC 
CTGACCCCAG GACCACCTAT AGCCTCTGGC AGCACAGGGA GCAGGGCCAC CAGTGCCCGA 
TGTAGGGGTG TCCCCCGGGG ACCTGTGAGA CCGGCTATCC CACCGCCACT GTCTTCGGTC 
TACGCTTGGG GTGGTGGCCT TAGCAGTCCC CTGAGGTTGC CAATGCTCAA CACGGAGGTG 
GTCCAGGTAG CCGCTGGGCG CACGCAGAAG GCTGGCGTTA CGCGCTCTGG ACGCCTCATC 
CTGTGGGAGG CCCCACCCCT GGGAGCTGGA GGCGGCACAC TCCTTCCTGG AGCCGTGGAG 
CTGCCTCAGC CCCAGTTTGT CTCTCGTTTC CTGGAAGGCC AGTCAGGTGT GACCATCAAG 
CATGTGGCCT GCGGGGACCT GTTCACCGCT TGCTTGACAG ACCGAGGCAT TATCATGACG 
TTCGGCAGTG GCAGCAATGG GTGTTTAGGT CACGGCAACC TGACTGACAT CAGCCAGCCC 
ACCATTGTAG AAGCCTTGCT GGGCTATGAG ATGGTGCAGG TGGCCTGTGG GGCCTCTCAT 
GTGCTGGCCC TGTCCACAGA TGGAGAGTTA TTCGCCTGGG GCAGAGGAGA TGGTGGTAGA 
CTGGGACTAG GCACCAGGGA GTCCCATAAC TGCCCTCAGC AGGTGCCTGT GGCCCCAGGA 
CAGGAAGCTC AGAGAGTTGT CTGTGGTATT GACTCTTCCA TGATCCTCAC CTCTCCAGGC 
AGGGTCCTGG CCTGTGGAAG TAACAGGTTC AACAAGCTAG GCTTGGACCA CCTATCCCTG 
GACGAGGAGC CTGTTCCCTA CCAGCAAGTG GAGGAGGCCC TGAGCTTCAC ACCACTGGGC 
TCTGCACCTC TGGACCAGGA GCCCCTGCTG TGTGTGGACC TTGGCACTGC TCATTCAGCT 
GCTATCACTG CTTCTGGGGA CTGCTATACT TTTGGCAGCA ATCAACATGG ACAGCTGGGC 
ACCAGTTCCC GCAGGGTCAG CAGGGCACCC TGTCGGGTCC AAGGCCTGGA GGGCATCAAG 
ATGGTGATGG TGGCCTGCGG GGATGCCTTC ACTGTGGCCG TTGGGGCAGA GGGGGAAGTG 
TATTCTTGGG GCAAAGGAAC CCGAGGCCGA CTGGGAAGGA GGGATGAGGA TGCGGGGCTG 
CCTAGGCCAG TGCAGCTGGA TGAGACTCAT CCTTACATGG TGACTTCAGT GTCCTGCTGC 
CATGGGAACA CTCTGCTGGC TGTTCGATTC ACAGATGAAC CAGTTCCCCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532247.1
CDS	90..2183
Translation	MEKYERIRVVGRGAFGIVHLCLRKADQKLVILKQIPVEQMTKEERQAAQNECQVLKLLNHPNVIEYYENFLEDKALMIAMEYAPGGTLAEFIQKRCNSLLEEETILHFFVQILLALHHVHTHLILHRDLKTQNILLDKHRMVVKIGDFGISKILSSKSKAYTVVGTPCYISPELCEGKPYNQKSDIWALGCVLYELASLKRAFEAANLPALVLKIMSGTFAPISDRYSPELRQLVLSLLSLEPAQRPPLSHIMAQPLCIRALLNIHTDVGSVRMRRAEKSLTPGPPIASGSTGSRATSARCRGVPRGPVRPAIPPPLSSVYAWGGGLSSPLRLPMLNTEVVQVAAGRTQKAGVTRSGRLILWEAPPLGAGGGTLLPGAVELPQPQFVSRFLEGQSGVTIKHVACGDLFTACLTDRGIIMTFGSGSNGCLGHGNLTDISQPTIVEALLGYEMVQVACGASHVLALSTDGELFAWGRGDGGRLGLGTRESHNCPQQVPVAPGQEAQRVVCGIDSSMILTSPGRVLACGSNRFNKLGLDHLSLDEEPVPYQQVEEALSFTPLGSAPLDQEPLLCVDLGTAHSAAITASGDCYTFGSNQHGQLGTSSRRVSRAPCRVQGLEGIKMVMVACGDAFTVAVGAEGEVYSWGKGTRGRLGRRDEDAGLPRPVQLDETHPYMVTSVSCCHGNTLLAVRFTDEPVPP

Target ORF information:

RefSeq Version	XM_006532184.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 8 (Nek8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532184.4

ORF Insert Sequence:

ATGGAGAAGT ACGAGCGGAT CCGAGTGGTG GGAAGAGGTG CCTTCGGGAT TGTGCACCTG 
TGCCTGCGAA AGGCCGACCA GAAGCTGGTG ATCCTCAAGC AGATCCCAGT GGAACAGATG 
ACCAAAGAAG AGAGGCAGGC GGCCCAGAAC GAGTGCCAGG TGCTCAAGCT GCTCAACCAC 
CCCAACGTCA TCGAGTACTA TGAGAATTTC CTAGAGGACA AGGCCCTCAT GATTGCTATG 
GAGTACGCAC CAGGTGGCAC CTTGGCCGAG TTCATCCAGA AGCGCTGCAA CTCTCTGCTA 
GAGGAGGAGA CGATCCTTCA CTTCTTTGTG CAGATCCTGC TGGCTCTGCA TCACGTGCAC 
ACACATCTCA TCCTGCATCG GGACCTCAAG ACACAGAACA TCCTTCTTGA CAAACACCGC 
ATGGTGGTCA AGATCGGTGA CTTTGGCATC TCTAAGATCC TCAGCAGCAA GAGCAAGGCC 
TACACGGTGG TGGGTACACC CTGCTACATC TCCCCTGAGC TGTGTGAGGG CAAACCCTAC 
AACCAGAAGA GTGACATCTG GGCTCTGGGC TGTGTCTTAT ATGAGCTGGC CAGCCTCAAG 
AGGGCCTTCG AGGCTGCAAA CTTGCCAGCC CTGGTGCTGA AGATCATGAG TGGCACCTTC 
GCACCCATCT CTGACCGGTA CAGCCCTGAG CTTCGCCAGC TCGTCCTGAG TCTGCTTAGC 
CTGGAACCTG CACAGAGGCC ACCCCTCAGC CACATCATGG CGCAGCCCCT CTGCATCCGG 
GCCCTACTCA ACATCCACAC CGATGTGGGC AGCGTGCGCA TGCGGAGGGC AGAGAAGTCC 
CTGACCCCAG GACCACCTAT AGCCTCTGGC AGCACAGGGA GCAGGGCCAC CAGTGCCCGA 
TGTAGGGGTG TCCCCCGGGG ACCTGTGAGA CCGGCTATCC CACCGCCACT GTCTTCGGTC 
TACGCTTGGG GTGGTGGCCT TAGCAGTCCC CTGAGGTTGC CAATGCTCAA CACGGAGGTG 
GTCCAGGTAG CCGCTGGGCG CACGCAGAAG GCTGGCGTTA CGCGCTCTGG ACGCCTCATC 
CTGTGGGAGG CCCCACCCCT GGGAGCTGGA GGCGGCACAC TCCTTCCTGG AGCCGTGGAG 
CTGCCTCAGC CCCAGTTTGT CTCTCGTTTC CTGGAAGGCC AGTCAGGTGT GACCATCAAG 
CATGTGGCCT GCGGGGACCT GTTCACCGCT TGCTTGACAG ACCGAGGCAT TATCATGACG 
TTCGGCAGTG GCAGCAATGG GTGTTTAGGT CACGGCAACC TGACTGACAT CAGCCAGCCC 
ACCATTGTAG AAGCCTTGCT GGGCTATGAG ATGGTGCAGG TGGCCTGTGG GGCCTCTCAT 
GTGCTGGCCC TGTCCACAGA TGGAGAGTTA TTCGCCTGGG GCAGAGGAGA TGGTGGTAGA 
CTGGGACTAG GCACCAGGGA GTCCCATAAC TGCCCTCAGC AGGTGCCTGT GGCCCCAGGA 
CAGGAAGCTC AGAGAGTTGT CTGTGGTATT GACTCTTCCA TGATCCTCAC CTCTCCAGGC 
AGGGTCCTGG CCTGTGGAAG TAACAGGTTC AACAAGCTAG GCTTGGACCA CCTATCCCTG 
GACGAGGAGC CTGTTCCCTA CCAGCAAGTG GAGGAGGCCC TGAGCTTCAC ACCACTGGGC 
TCTGCACCTC TGGACCAGGA GCCCCTGCTG TGTGTGGACC TTGGCACTGC TCATTCAGCT 
GCTATCACTG CTTCTGGGGA CTGCTATACT TTTGGCAGCA ATCAACATGG ACAGCTGGGC 
ACCAGTTCCC GCAGGGTCAG CAGGGCACCC TGTCGGGTCC AAGGCCTGGA GGGCATCAAG 
ATGGTGATGG TGGCCTGCGG GGATGCCTTC ACTGTGGCCG TTGGGGCAGA GGGGGAAGTG 
TATTCTTGGG GCAAAGGAAC CCGAGGCCGA CTGGGAAGGA GGGATGAGGA TGCGGGGCTG 
CCTAGGCCAG TGCAGCTGGA TGAGACTCAT CCTTACATGG TGACTTCAGT GTCCTGCTGC 
CATGGGAACA CTCTGCTGGC TGTTCGATTC ACAGATGAAC CAGTTCCCCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nek8 ( XM_006532185.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532248.1 Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 8 (Nek8), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu45549
Clone ID Related Accession (Same CDS sequence)	XM_006532185.4 , XM_006532185.3
Accession Version	XM_006532185.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1977bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase Nek8 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006532185.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGT ACGAGCGGAT CCGAGTGGTG GGAAGAGGTG CCTTCGGGAT TGTGCACCTG 
TGCCTGCGAA AGGCCGACCA GAAGCTGGTG ATCCTCAAGC AGATCCCAGT GGAACAGATG 
ACCAAAGAAG AGAGGCAGGC GGCCCAGAAC GAGTGCCAGG TGCTCAAGCT GCTCAACCAC 
CCCAACGTCA TCGAGTACTA TGAGAATTTC CTAGAGGACA AGGCCCTCAT GATTGCTATG 
GAGTACGCAC CAGGTGGCAC CTTGGCCGAG TTCATCCAGA AGCGCTGCAA CTCTCTGCTA 
GAGGAGGAGA CGATCCTTCA CTTCTTTGTG CAGATCCTGC TGGCTCTGCA TCACGTGCAC 
ACACATCTCA TCCTGCATCG GGACCTCAAG ACACAGAACA TCCTTCTTGA CAAACACCGC 
ATGGTGGTCA AGATCGGTGA CTTTGGCATC TCTAAGATCC TCAGCAGCAA GAGCAAGGCC 
TACACGAACT TGCCAGCCCT GGTGCTGAAG ATCATGAGTG GCACCTTCGC ACCCATCTCT 
GACCGGTACA GCCCTGAGCT TCGCCAGCTC GTCCTGAGTC TGCTTAGCCT GGAACCTGCA 
CAGAGGCCAC CCCTCAGCCA CATCATGGCG CAGCCCCTCT GCATCCGGGC CCTACTCAAC 
ATCCACACCG ATGTGGGCAG CGTGCGCATG CGGAGGGCAG AGAAGTCCCT GACCCCAGGA 
CCACCTATAG CCTCTGGCAG CACAGGGAGC AGGGCCACCA GTGCCCGATG TAGGGGTGTC 
CCCCGGGGAC CTGTGAGACC GGCTATCCCA CCGCCACTGT CTTCGGTCTA CGCTTGGGGT 
GGTGGCCTTA GCAGTCCCCT GAGGTTGCCA ATGCTCAACA CGGAGGTGGT CCAGGTAGCC 
GCTGGGCGCA CGCAGAAGGC TGGCGTTACG CGCTCTGGAC GCCTCATCCT GTGGGAGGCC 
CCACCCCTGG GAGCTGGAGG CGGCACACTC CTTCCTGGAG CCGTGGAGCT GCCTCAGCCC 
CAGTTTGTCT CTCGTTTCCT GGAAGGCCAG TCAGGTGTGA CCATCAAGCA TGTGGCCTGC 
GGGGACCTGT TCACCGCTTG CTTGACAGAC CGAGGCATTA TCATGACGTT CGGCAGTGGC 
AGCAATGGGT GTTTAGGTCA CGGCAACCTG ACTGACATCA GCCAGCCCAC CATTGTAGAA 
GCCTTGCTGG GCTATGAGAT GGTGCAGGTG GCCTGTGGGG CCTCTCATGT GCTGGCCCTG 
TCCACAGATG GAGAGTTATT CGCCTGGGGC AGAGGAGATG GTGGTAGACT GGGACTAGGC 
ACCAGGGAGT CCCATAACTG CCCTCAGCAG GTGCCTGTGG CCCCAGGACA GGAAGCTCAG 
AGAGTTGTCT GTGGTATTGA CTCTTCCATG ATCCTCACCT CTCCAGGCAG GGTCCTGGCC 
TGTGGAAGTA ACAGGTTCAA CAAGCTAGGC TTGGACCACC TATCCCTGGA CGAGGAGCCT 
GTTCCCTACC AGCAAGTGGA GGAGGCCCTG AGCTTCACAC CACTGGGCTC TGCACCTCTG 
GACCAGGAGC CCCTGCTGTG TGTGGACCTT GGCACTGCTC ATTCAGCTGC TATCACTGCT 
TCTGGGGACT GCTATACTTT TGGCAGCAAT CAACATGGAC AGCTGGGCAC CAGTTCCCGC 
AGGGTCAGCA GGGCACCCTG TCGGGTCCAA GGCCTGGAGG GCATCAAGAT GGTGATGGTG 
GCCTGCGGGG ATGCCTTCAC TGTGGCCGTT GGGGCAGAGG GGGAAGTGTA TTCTTGGGGC 
AAAGGAACCC GAGGCCGACT GGGAAGGAGG GATGAGGATG CGGGGCTGCC TAGGCCAGTG 
CAGCTGGATG AGACTCATCC TTACATGGTG ACTTCAGTGT CCTGCTGCCA TGGGAACACT 
CTGCTGGCTG TTCGATGTGA GTTTTCTTCT CCTATCTCAC CCACATCACT AGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532248.1
CDS	90..2066
Translation	MEKYERIRVVGRGAFGIVHLCLRKADQKLVILKQIPVEQMTKEERQAAQNECQVLKLLNHPNVIEYYENFLEDKALMIAMEYAPGGTLAEFIQKRCNSLLEEETILHFFVQILLALHHVHTHLILHRDLKTQNILLDKHRMVVKIGDFGISKILSSKSKAYTNLPALVLKIMSGTFAPISDRYSPELRQLVLSLLSLEPAQRPPLSHIMAQPLCIRALLNIHTDVGSVRMRRAEKSLTPGPPIASGSTGSRATSARCRGVPRGPVRPAIPPPLSSVYAWGGGLSSPLRLPMLNTEVVQVAAGRTQKAGVTRSGRLILWEAPPLGAGGGTLLPGAVELPQPQFVSRFLEGQSGVTIKHVACGDLFTACLTDRGIIMTFGSGSNGCLGHGNLTDISQPTIVEALLGYEMVQVACGASHVLALSTDGELFAWGRGDGGRLGLGTRESHNCPQQVPVAPGQEAQRVVCGIDSSMILTSPGRVLACGSNRFNKLGLDHLSLDEEPVPYQQVEEALSFTPLGSAPLDQEPLLCVDLGTAHSAAITASGDCYTFGSNQHGQLGTSSRRVSRAPCRVQGLEGIKMVMVACGDAFTVAVGAEGEVYSWGKGTRGRLGRRDEDAGLPRPVQLDETHPYMVTSVSCCHGNTLLAVRCEFSSPISPTSLG

Target ORF information:

RefSeq Version	XM_006532185.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 8 (Nek8), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532185.4

ORF Insert Sequence:

ATGGAGAAGT ACGAGCGGAT CCGAGTGGTG GGAAGAGGTG CCTTCGGGAT TGTGCACCTG 
TGCCTGCGAA AGGCCGACCA GAAGCTGGTG ATCCTCAAGC AGATCCCAGT GGAACAGATG 
ACCAAAGAAG AGAGGCAGGC GGCCCAGAAC GAGTGCCAGG TGCTCAAGCT GCTCAACCAC 
CCCAACGTCA TCGAGTACTA TGAGAATTTC CTAGAGGACA AGGCCCTCAT GATTGCTATG 
GAGTACGCAC CAGGTGGCAC CTTGGCCGAG TTCATCCAGA AGCGCTGCAA CTCTCTGCTA 
GAGGAGGAGA CGATCCTTCA CTTCTTTGTG CAGATCCTGC TGGCTCTGCA TCACGTGCAC 
ACACATCTCA TCCTGCATCG GGACCTCAAG ACACAGAACA TCCTTCTTGA CAAACACCGC 
ATGGTGGTCA AGATCGGTGA CTTTGGCATC TCTAAGATCC TCAGCAGCAA GAGCAAGGCC 
TACACGAACT TGCCAGCCCT GGTGCTGAAG ATCATGAGTG GCACCTTCGC ACCCATCTCT 
GACCGGTACA GCCCTGAGCT TCGCCAGCTC GTCCTGAGTC TGCTTAGCCT GGAACCTGCA 
CAGAGGCCAC CCCTCAGCCA CATCATGGCG CAGCCCCTCT GCATCCGGGC CCTACTCAAC 
ATCCACACCG ATGTGGGCAG CGTGCGCATG CGGAGGGCAG AGAAGTCCCT GACCCCAGGA 
CCACCTATAG CCTCTGGCAG CACAGGGAGC AGGGCCACCA GTGCCCGATG TAGGGGTGTC 
CCCCGGGGAC CTGTGAGACC GGCTATCCCA CCGCCACTGT CTTCGGTCTA CGCTTGGGGT 
GGTGGCCTTA GCAGTCCCCT GAGGTTGCCA ATGCTCAACA CGGAGGTGGT CCAGGTAGCC 
GCTGGGCGCA CGCAGAAGGC TGGCGTTACG CGCTCTGGAC GCCTCATCCT GTGGGAGGCC 
CCACCCCTGG GAGCTGGAGG CGGCACACTC CTTCCTGGAG CCGTGGAGCT GCCTCAGCCC 
CAGTTTGTCT CTCGTTTCCT GGAAGGCCAG TCAGGTGTGA CCATCAAGCA TGTGGCCTGC 
GGGGACCTGT TCACCGCTTG CTTGACAGAC CGAGGCATTA TCATGACGTT CGGCAGTGGC 
AGCAATGGGT GTTTAGGTCA CGGCAACCTG ACTGACATCA GCCAGCCCAC CATTGTAGAA 
GCCTTGCTGG GCTATGAGAT GGTGCAGGTG GCCTGTGGGG CCTCTCATGT GCTGGCCCTG 
TCCACAGATG GAGAGTTATT CGCCTGGGGC AGAGGAGATG GTGGTAGACT GGGACTAGGC 
ACCAGGGAGT CCCATAACTG CCCTCAGCAG GTGCCTGTGG CCCCAGGACA GGAAGCTCAG 
AGAGTTGTCT GTGGTATTGA CTCTTCCATG ATCCTCACCT CTCCAGGCAG GGTCCTGGCC 
TGTGGAAGTA ACAGGTTCAA CAAGCTAGGC TTGGACCACC TATCCCTGGA CGAGGAGCCT 
GTTCCCTACC AGCAAGTGGA GGAGGCCCTG AGCTTCACAC CACTGGGCTC TGCACCTCTG 
GACCAGGAGC CCCTGCTGTG TGTGGACCTT GGCACTGCTC ATTCAGCTGC TATCACTGCT 
TCTGGGGACT GCTATACTTT TGGCAGCAAT CAACATGGAC AGCTGGGCAC CAGTTCCCGC 
AGGGTCAGCA GGGCACCCTG TCGGGTCCAA GGCCTGGAGG GCATCAAGAT GGTGATGGTG 
GCCTGCGGGG ATGCCTTCAC TGTGGCCGTT GGGGCAGAGG GGGAAGTGTA TTCTTGGGGC 
AAAGGAACCC GAGGCCGACT GGGAAGGAGG GATGAGGATG CGGGGCTGCC TAGGCCAGTG 
CAGCTGGATG AGACTCATCC TTACATGGTG ACTTCAGTGT CCTGCTGCCA TGGGAACACT 
CTGCTGGCTG TTCGATGTGA GTTTTCTTCT CCTATCTCAC CCACATCACT AGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Distinct oxylipin alterations in diverse models of cystic kidney diseases.
Biochimica et biophysica acta1862(12)1562-1574(2017 12)
Monirujjaman M,Devassy JG,Yamaguchi T,Sidhu N,Kugita M,Gabbs M,Nagao S,Zhou J,Ravandi A,Aukema HM

NEK8 regulates DNA damage-induced RAD51 foci formation and replication fork protection.
Cell cycle (Georgetown, Tex.)16(4)335-347(2017 Feb)
Abeyta A,Castella M,Jacquemont C,Taniguchi T

CaMKII as a pathological mediator of ER stress, oxidative stress, and mitochondrial dysfunction in a murine model of nephronophthisis.
American journal of physiology. Renal physiology310(11)F1414-22(2016 Jun)
Bracken C,Beauverger P,Duclos O,Russo RJ,Rogers KA,Husson H,Natoli TA,Ledbetter SR,Janiak P,Ibraghimov-Beskrovnaya O,Bukanov NO

High-resolution genetic localization of a modifying locus affecting disease severity in the juvenile cystic kidneys (jck) mouse model of polycystic kidney disease.
Mammalian genome : official journal of the International Mammalian Genome Society27(5-6)191-9(2016 Jun)
Beier DR

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Nek8 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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