nekl-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	nekl-1
Entrez Gene ID	189769
Full Name	NEK (NEver in mitosis Kinase) Like
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_001306392.1	NP_001293321.1	NEK (NEver in mitosis Kinase) Like
NM_001306391.2	NP_001293320.1	Protein kinase domain-containing protein
NM_001306391.1	NP_001293320.1	NEK (NEver in mitosis Kinase) Like
NM_001306392.2	NP_001293321.1	Protein kinase domain-containing protein

Pathways from BioSystems

Homologies

	NEK8	XP_001106986.2
Drosophila melanogaster (fruit fly)	niki	NP_651293.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006872	XP_308885.4
Canis lupus familiaris (dog)	NEK8	XP_548291.3
Pan troglodytes (chimpanzee)	NEK8	XP_511801.3
Mus musculus (house mouse)	Nek8	NP_543125.1
Homo sapiens (human)	NEK8	NP_835464.1
Rattus norvegicus (Norway rat)	Nek8	NP_001099274.1
Danio rerio (zebrafish)	nek8	NP_620776.1
Bos taurus (cattle)	NEK8	NP_001179652.1
Gallus gallus (chicken)	NEK8	XP_415822.3
Caenorhabditis elegans (roundworm)	nekl-1	NP_490968.2
Xenopus tropicalis (tropical clawed frog)	nek8	NP_001006906.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following nekl-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nekl-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa336146	NM_001306391.2 Latest version!	Caenorhabditis elegans Protein kinase domain-containing protein (nekl-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OCa336147	NM_001306392.2 Latest version!	Caenorhabditis elegans Protein kinase domain-containing protein (nekl-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OCa336146	NM_001306391.1	Caenorhabditis elegans NEK (NEver in mitosis Kinase) Like (nekl-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OCa336147	NM_001306392.1	Caenorhabditis elegans NEK (NEver in mitosis Kinase) Like (nekl-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nekl-1 ( NM_001306391.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001293320.1 Caenorhabditis elegans Protein kinase domain-containing protein (nekl-1), partial mRNA.

Price & Availability↑

CloneID	OCa336146
Clone ID Related Accession (Same CDS sequence)	NM_001306391.2 , NM_001306391.1
Accession Version	NM_001306391.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2952bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Protein kinase domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_001306391.1. COMPLETENESS: incomplete on the 5' end.

ATGAGCACGG TGATTCTTTG CCATGCAGAA TCTGTAAATT TGGATACGAT TCGAGCTTCC 
GATGCAAATA TTATTAGATA CAGTGTTGGT GTGAAAATGA CCGAGTTGAA AGACATGCTA 
AAACAATACA CTGCTGGCCG AATTCGGAAC CTCGGCCTCC ATATCCAAAC CCGCGACACG 
AAACTTCGAA TATTAGAGGA TATGGAAGTA ACGAAAGAAT CCCTTGAAAA CGCCGGCTCA 
TTCGTCTCCC AATTTTTTCG ATGGCTCATT TCAGAAGTAT TGATCGAGGG GGCGTTTATC 
GATTTTTTGC TGACCACGCC CATCGAAAAC GCGTCGCAAA TTTGTTTGGC AGTGCAGAGT 
GTTAGCAAGA AAAAGATTTC CGTGCGTTTG GTAAGCAGAT TTTTGTCGTC CGAGCTGGCT 
CCCCATAACA GAAGAATCGT CGAGATTTAT ATCAATAATG AGATAATGAT AACAAACCCG 
AATCCGAGAG CCACATCGGC ACAAAATCGA TATGAACGTA TTCGGACTGT TGGAAAAGGT 
GCCTTCGGGT CCGCCGTGCT CTACCGCCGA AGAGAAGACA GTTCGTTGGT GATTATAAAA 
GAAATCAATA TGTACGATTT GGATAGTTCA CAGAGGAGAT TAGCGCTGAA TGAGGTTTCA 
CTGTTATCAC GTATCGAGCA CCCCAATATA ATTGCATACT ATGATTCATT TGAAGAAGAA 
GGTGTTTTAA TGATTGAAAT GGAATATGCT GATGGTGGAA CACTTGCTCA AATGTTATCA 
AGAACACAGA ATTTGTTGGA TGAAGAGCAA ATTGGCGATA TGATGATTCA AATGTGCTCG 
GCGGTGGCCT ACTTGCATGA GAATAGTGTT TTGCACAGAG ACCTAAAAAC TGCCAACGTC 
TTCCTAACTC GAGACTCATT TGTAAAAATC GGCGACTTTG GAATCTCCAA AATAATGGGC 
ACTGAAACAT TGGCTCAAGG AGCAAAAACA GTTGTCGGAA CACCGTATTA CATAAGTCCA 
GAGATGTGTT CAGGTGTCTC TTATAATGAG AAAAGTGATA TGTGGGCTCT TGGATGTATT 
CTTTATGAAA TGTGTTGTTT GAAGAAAGCG TTTGAAGGTG ACAATCTTCC CGCGTTGGTC 
AATAGTATTA TGACGTGTGC CTACACACCA GTAAAAGGAC CTTACTCGGC TGAAATGAAA 
ATGGTGATAC GGGAATTGCT ACAGTTGGAT CCGCAAAAGA GGCCCTCCGC TCCGCAGGCG 
CTAAAGATGC TGCGACCTTC GGAAAACCGT CACCGACACA CCTCCGGATC AATGAGATCT 
TCGGTTTCGT TCACAACTCT CTACGATTTG CATGTCCCGA CAATCACCCT GTCCTCAATT 
CCAGACCTTC CCAGCCGGAT ATCGATTAAG CAGGTCGCTC TGAGCAAAAC TCATACAATG 
GTGCTCACAA ATGATAATGA ACTTTTCGGA TTTGGTGATA ACTCTTGTGG ACAACTGGGA 
TTCGGGAAGT TTTCGAAAAT GGAAAACGGG CGGTTGGTGG AGAGTTTGAA GGGCAGAGAT 
ATACAATCAG TTGGGGTTGG GAATTATTTT AGTGTAATCT GCTGTGATCG GGGGACCTTA 
TTGACATTTG GTACTGCAAA GAACCTCGGT TTGGGGAAAG ATGCAAAAGA TTCAGCGAAG 
CCGATGCTCC TCGAGCAACT TTTGAGAGAA AATATCAAAG ACATATATTG CGGTCATGAC 
CATGTGGTGG CCGTATTGGA AAGTGGTGAA TGTCTAGGAT GGGGAAGTAA TCAATACGGA 
CAACTCGGGC TGCCAACGTT GGAGCACTTT TTTGCTCCGA CTAAGATTCC GATGCCGAGC 
AACAAGAAAA TCGTGTACGG GAAGGCTGGG AAAGATGCGA CGATGCTATT ATCGGATGAT 
GGAAGTTTGA TAGCAATGGG CTCAAATAAG CATAATAAGC TGAATCTTAC ACAGAGGGTC 
GGGTTCTTTA CAAAAGAGAA AAAGACACCA AACGATTATG TCAACAAGCC GACGATTCTT 
CAAGGATTCC CGGAGCGAGT CGTCGATTTC TCGGTTGGAA GTTATCATTC GGGAGTTATA 
CTGGAATCCG GTCAAGTGAT GATTTTCGGC CGTAATGACA ACACTGAACT CGGCCTCGGG 
AATAGCCAAA AAATGGCGGT TCGATGTGTG AAACCGGTCA AATCGCTGCT TCATCACGCC 
TGCACGCACG TACTCTGCGG TGATGGGTTC ACTCTGGTGA GCACAACGGA GCAGGAACTC 
TACTTTTGGG GACGAAAGGA TCTGACAGAC GAAGATGTGT GTATTGATGA TCTTGAAGAA 
ACCGTCACTG AAAAAAAGAT GAAGCGGGGC ACCACTACTG AGATTTTCGA CAAAAATGTG 
GTCACTTTAC CGTCGTTGAT TCTCAGGCTT CAAGATGAAA GAGGCGACAA AGGGATTCGG 
CTCTCCGGTC TGGCAGTTTC CGGCCGAAAA GTGCATGTGG CAGTGGATAT GCTCGAAGGG 
AAAAGACTTC CTTCCGCTCT CGCAGAAAGG TGCAGAAGTG GGTCGGCGCC AGCGATCAAC 
GACGAGACGA CTCATACTTG GCTCCGAGAG GAGTTCGAGA AGGCTGAAAT TATACCAATT 
GACGCGTCGA AACGACGAGT TCGGTTTGAT GATTCTTGTG AGAAGCTGAA AATTATGGAC 
GCGACTGCCA AACAAAACTC ATTGCTCCGC GAGATCGAGG ACCTAAAATC GAAAATTAAA 
GAGCAAGACA CCACATTCGA AGGTCACCGC AATCAAATGT CCGATTTGGA GTCAAAACTG 
CAAGAGCTTC AAGCCAAACA GTCATTCCTC CGGAAATCCG AACCACCTCC AGCCTATGAT 
CGACGGAATG TCACGTATAA CAAACTGTTT CCAGAAAGGG AGAATACGAG TACAACATGT 
GTTATTTTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001293320.1
CDS	1..2952
Translation	MSTVILCHAESVNLDTIRASDANIIRYSVGVKMTELKDMLKQYTAGRIRNLGLHIQTRDTKLRILEDMEVTKESLENAGSFVSQFFRWLISEVLIEGAFIDFLLTTPIENASQICLAVQSVSKKKISVRLVSRFLSSELAPHNRRIVEIYINNEIMITNPNPRATSAQNRYERIRTVGKGAFGSAVLYRRREDSSLVIIKEINMYDLDSSQRRLALNEVSLLSRIEHPNIIAYYDSFEEEGVLMIEMEYADGGTLAQMLSRTQNLLDEEQIGDMMIQMCSAVAYLHENSVLHRDLKTANVFLTRDSFVKIGDFGISKIMGTETLAQGAKTVVGTPYYISPEMCSGVSYNEKSDMWALGCILYEMCCLKKAFEGDNLPALVNSIMTCAYTPVKGPYSAEMKMVIRELLQLDPQKRPSAPQALKMLRPSENRHRHTSGSMRSSVSFTTLYDLHVPTITLSSIPDLPSRISIKQVALSKTHTMVLTNDNELFGFGDNSCGQLGFGKFSKMENGRLVESLKGRDIQSVGVGNYFSVICCDRGTLLTFGTAKNLGLGKDAKDSAKPMLLEQLLRENIKDIYCGHDHVVAVLESGECLGWGSNQYGQLGLPTLEHFFAPTKIPMPSNKKIVYGKAGKDATMLLSDDGSLIAMGSNKHNKLNLTQRVGFFTKEKKTPNDYVNKPTILQGFPERVVDFSVGSYHSGVILESGQVMIFGRNDNTELGLGNSQKMAVRCVKPVKSLLHHACTHVLCGDGFTLVSTTEQELYFWGRKDLTDEDVCIDDLEETVTEKKMKRGTTTEIFDKNVVTLPSLILRLQDERGDKGIRLSGLAVSGRKVHVAVDMLEGKRLPSALAERCRSGSAPAINDETTHTWLREEFEKAEIIPIDASKRRVRFDDSCEKLKIMDATAKQNSLLREIEDLKSKIKEQDTTFEGHRNQMSDLESKLQELQAKQSFLRKSEPPPAYDRRNVTYNKLFPERENTSTTCVIL

Target ORF information:

RefSeq Version	NM_001306391.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Protein kinase domain-containing protein (nekl-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001306391.2

ORF Insert Sequence:

ATGAGCACGG TGATTCTTTG CCATGCAGAA TCTGTAAATT TGGATACGAT TCGAGCTTCC 
GATGCAAATA TTATTAGATA CAGTGTTGGT GTGAAAATGA CCGAGTTGAA AGACATGCTA 
AAACAATACA CTGCTGGCCG AATTCGGAAC CTCGGCCTCC ATATCCAAAC CCGCGACACG 
AAACTTCGAA TATTAGAGGA TATGGAAGTA ACGAAAGAAT CCCTTGAAAA CGCCGGCTCA 
TTCGTCTCCC AATTTTTTCG ATGGCTCATT TCAGAAGTAT TGATCGAGGG GGCGTTTATC 
GATTTTTTGC TGACCACGCC CATCGAAAAC GCGTCGCAAA TTTGTTTGGC AGTGCAGAGT 
GTTAGCAAGA AAAAGATTTC CGTGCGTTTG GTAAGCAGAT TTTTGTCGTC CGAGCTGGCT 
CCCCATAACA GAAGAATCGT CGAGATTTAT ATCAATAATG AGATAATGAT AACAAACCCG 
AATCCGAGAG CCACATCGGC ACAAAATCGA TATGAACGTA TTCGGACTGT TGGAAAAGGT 
GCCTTCGGGT CCGCCGTGCT CTACCGCCGA AGAGAAGACA GTTCGTTGGT GATTATAAAA 
GAAATCAATA TGTACGATTT GGATAGTTCA CAGAGGAGAT TAGCGCTGAA TGAGGTTTCA 
CTGTTATCAC GTATCGAGCA CCCCAATATA ATTGCATACT ATGATTCATT TGAAGAAGAA 
GGTGTTTTAA TGATTGAAAT GGAATATGCT GATGGTGGAA CACTTGCTCA AATGTTATCA 
AGAACACAGA ATTTGTTGGA TGAAGAGCAA ATTGGCGATA TGATGATTCA AATGTGCTCG 
GCGGTGGCCT ACTTGCATGA GAATAGTGTT TTGCACAGAG ACCTAAAAAC TGCCAACGTC 
TTCCTAACTC GAGACTCATT TGTAAAAATC GGCGACTTTG GAATCTCCAA AATAATGGGC 
ACTGAAACAT TGGCTCAAGG AGCAAAAACA GTTGTCGGAA CACCGTATTA CATAAGTCCA 
GAGATGTGTT CAGGTGTCTC TTATAATGAG AAAAGTGATA TGTGGGCTCT TGGATGTATT 
CTTTATGAAA TGTGTTGTTT GAAGAAAGCG TTTGAAGGTG ACAATCTTCC CGCGTTGGTC 
AATAGTATTA TGACGTGTGC CTACACACCA GTAAAAGGAC CTTACTCGGC TGAAATGAAA 
ATGGTGATAC GGGAATTGCT ACAGTTGGAT CCGCAAAAGA GGCCCTCCGC TCCGCAGGCG 
CTAAAGATGC TGCGACCTTC GGAAAACCGT CACCGACACA CCTCCGGATC AATGAGATCT 
TCGGTTTCGT TCACAACTCT CTACGATTTG CATGTCCCGA CAATCACCCT GTCCTCAATT 
CCAGACCTTC CCAGCCGGAT ATCGATTAAG CAGGTCGCTC TGAGCAAAAC TCATACAATG 
GTGCTCACAA ATGATAATGA ACTTTTCGGA TTTGGTGATA ACTCTTGTGG ACAACTGGGA 
TTCGGGAAGT TTTCGAAAAT GGAAAACGGG CGGTTGGTGG AGAGTTTGAA GGGCAGAGAT 
ATACAATCAG TTGGGGTTGG GAATTATTTT AGTGTAATCT GCTGTGATCG GGGGACCTTA 
TTGACATTTG GTACTGCAAA GAACCTCGGT TTGGGGAAAG ATGCAAAAGA TTCAGCGAAG 
CCGATGCTCC TCGAGCAACT TTTGAGAGAA AATATCAAAG ACATATATTG CGGTCATGAC 
CATGTGGTGG CCGTATTGGA AAGTGGTGAA TGTCTAGGAT GGGGAAGTAA TCAATACGGA 
CAACTCGGGC TGCCAACGTT GGAGCACTTT TTTGCTCCGA CTAAGATTCC GATGCCGAGC 
AACAAGAAAA TCGTGTACGG GAAGGCTGGG AAAGATGCGA CGATGCTATT ATCGGATGAT 
GGAAGTTTGA TAGCAATGGG CTCAAATAAG CATAATAAGC TGAATCTTAC ACAGAGGGTC 
GGGTTCTTTA CAAAAGAGAA AAAGACACCA AACGATTATG TCAACAAGCC GACGATTCTT 
CAAGGATTCC CGGAGCGAGT CGTCGATTTC TCGGTTGGAA GTTATCATTC GGGAGTTATA 
CTGGAATCCG GTCAAGTGAT GATTTTCGGC CGTAATGACA ACACTGAACT CGGCCTCGGG 
AATAGCCAAA AAATGGCGGT TCGATGTGTG AAACCGGTCA AATCGCTGCT TCATCACGCC 
TGCACGCACG TACTCTGCGG TGATGGGTTC ACTCTGGTGA GCACAACGGA GCAGGAACTC 
TACTTTTGGG GACGAAAGGA TCTGACAGAC GAAGATGTGT GTATTGATGA TCTTGAAGAA 
ACCGTCACTG AAAAAAAGAT GAAGCGGGGC ACCACTACTG AGATTTTCGA CAAAAATGTG 
GTCACTTTAC CGTCGTTGAT TCTCAGGCTT CAAGATGAAA GAGGCGACAA AGGGATTCGG 
CTCTCCGGTC TGGCAGTTTC CGGCCGAAAA GTGCATGTGG CAGTGGATAT GCTCGAAGGG 
AAAAGACTTC CTTCCGCTCT CGCAGAAAGG TGCAGAAGTG GGTCGGCGCC AGCGATCAAC 
GACGAGACGA CTCATACTTG GCTCCGAGAG GAGTTCGAGA AGGCTGAAAT TATACCAATT 
GACGCGTCGA AACGACGAGT TCGGTTTGAT GATTCTTGTG AGAAGCTGAA AATTATGGAC 
GCGACTGCCA AACAAAACTC ATTGCTCCGC GAGATCGAGG ACCTAAAATC GAAAATTAAA 
GAGCAAGACA CCACATTCGA AGGTCACCGC AATCAAATGT CCGATTTGGA GTCAAAACTG 
CAAGAGCTTC AAGCCAAACA GTCATTCCTC CGGAAATCCG AACCACCTCC AGCCTATGAT 
CGACGGAATG TCACGTATAA CAAACTGTTT CCAGAAAGGG AGAATACGAG TACAACATGT 
GTTATTTTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nekl-1 ( NM_001306391.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001293320.1 Caenorhabditis elegans NEK (NEver in mitosis Kinase) Like (nekl-1), partial mRNA.

Price & Availability↑

CloneID	OCa336146
Clone ID Related Accession (Same CDS sequence)	NM_001306391.2 , NM_001306391.1
Accession Version	NM_001306391.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2952bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	NEK (NEver in mitosis Kinase) Like
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). COMPLETENESS: incomplete on both ends.

ATGAGCACGG TGATTCTTTG CCATGCAGAA TCTGTAAATT TGGATACGAT TCGAGCTTCC 
GATGCAAATA TTATTAGATA CAGTGTTGGT GTGAAAATGA CCGAGTTGAA AGACATGCTA 
AAACAATACA CTGCTGGCCG AATTCGGAAC CTCGGCCTCC ATATCCAAAC CCGCGACACG 
AAACTTCGAA TATTAGAGGA TATGGAAGTA ACGAAAGAAT CCCTTGAAAA CGCCGGCTCA 
TTCGTCTCCC AATTTTTTCG ATGGCTCATT TCAGAAGTAT TGATCGAGGG GGCGTTTATC 
GATTTTTTGC TGACCACGCC CATCGAAAAC GCGTCGCAAA TTTGTTTGGC AGTGCAGAGT 
GTTAGCAAGA AAAAGATTTC CGTGCGTTTG GTAAGCAGAT TTTTGTCGTC CGAGCTGGCT 
CCCCATAACA GAAGAATCGT CGAGATTTAT ATCAATAATG AGATAATGAT AACAAACCCG 
AATCCGAGAG CCACATCGGC ACAAAATCGA TATGAACGTA TTCGGACTGT TGGAAAAGGT 
GCCTTCGGGT CCGCCGTGCT CTACCGCCGA AGAGAAGACA GTTCGTTGGT GATTATAAAA 
GAAATCAATA TGTACGATTT GGATAGTTCA CAGAGGAGAT TAGCGCTGAA TGAGGTTTCA 
CTGTTATCAC GTATCGAGCA CCCCAATATA ATTGCATACT ATGATTCATT TGAAGAAGAA 
GGTGTTTTAA TGATTGAAAT GGAATATGCT GATGGTGGAA CACTTGCTCA AATGTTATCA 
AGAACACAGA ATTTGTTGGA TGAAGAGCAA ATTGGCGATA TGATGATTCA AATGTGCTCG 
GCGGTGGCCT ACTTGCATGA GAATAGTGTT TTGCACAGAG ACCTAAAAAC TGCCAACGTC 
TTCCTAACTC GAGACTCATT TGTAAAAATC GGCGACTTTG GAATCTCCAA AATAATGGGC 
ACTGAAACAT TGGCTCAAGG AGCAAAAACA GTTGTCGGAA CACCGTATTA CATAAGTCCA 
GAGATGTGTT CAGGTGTCTC TTATAATGAG AAAAGTGATA TGTGGGCTCT TGGATGTATT 
CTTTATGAAA TGTGTTGTTT GAAGAAAGCG TTTGAAGGTG ACAATCTTCC CGCGTTGGTC 
AATAGTATTA TGACGTGTGC CTACACACCA GTAAAAGGAC CTTACTCGGC TGAAATGAAA 
ATGGTGATAC GGGAATTGCT ACAGTTGGAT CCGCAAAAGA GGCCCTCCGC TCCGCAGGCG 
CTAAAGATGC TGCGACCTTC GGAAAACCGT CACCGACACA CCTCCGGATC AATGAGATCT 
TCGGTTTCGT TCACAACTCT CTACGATTTG CATGTCCCGA CAATCACCCT GTCCTCAATT 
CCAGACCTTC CCAGCCGGAT ATCGATTAAG CAGGTCGCTC TGAGCAAAAC TCATACAATG 
GTGCTCACAA ATGATAATGA ACTTTTCGGA TTTGGTGATA ACTCTTGTGG ACAACTGGGA 
TTCGGGAAGT TTTCGAAAAT GGAAAACGGG CGGTTGGTGG AGAGTTTGAA GGGCAGAGAT 
ATACAATCAG TTGGGGTTGG GAATTATTTT AGTGTAATCT GCTGTGATCG GGGGACCTTA 
TTGACATTTG GTACTGCAAA GAACCTCGGT TTGGGGAAAG ATGCAAAAGA TTCAGCGAAG 
CCGATGCTCC TCGAGCAACT TTTGAGAGAA AATATCAAAG ACATATATTG CGGTCATGAC 
CATGTGGTGG CCGTATTGGA AAGTGGTGAA TGTCTAGGAT GGGGAAGTAA TCAATACGGA 
CAACTCGGGC TGCCAACGTT GGAGCACTTT TTTGCTCCGA CTAAGATTCC GATGCCGAGC 
AACAAGAAAA TCGTGTACGG GAAGGCTGGG AAAGATGCGA CGATGCTATT ATCGGATGAT 
GGAAGTTTGA TAGCAATGGG CTCAAATAAG CATAATAAGC TGAATCTTAC ACAGAGGGTC 
GGGTTCTTTA CAAAAGAGAA AAAGACACCA AACGATTATG TCAACAAGCC GACGATTCTT 
CAAGGATTCC CGGAGCGAGT CGTCGATTTC TCGGTTGGAA GTTATCATTC GGGAGTTATA 
CTGGAATCCG GTCAAGTGAT GATTTTCGGC CGTAATGACA ACACTGAACT CGGCCTCGGG 
AATAGCCAAA AAATGGCGGT TCGATGTGTG AAACCGGTCA AATCGCTGCT TCATCACGCC 
TGCACGCACG TACTCTGCGG TGATGGGTTC ACTCTGGTGA GCACAACGGA GCAGGAACTC 
TACTTTTGGG GACGAAAGGA TCTGACAGAC GAAGATGTGT GTATTGATGA TCTTGAAGAA 
ACCGTCACTG AAAAAAAGAT GAAGCGGGGC ACCACTACTG AGATTTTCGA CAAAAATGTG 
GTCACTTTAC CGTCGTTGAT TCTCAGGCTT CAAGATGAAA GAGGCGACAA AGGGATTCGG 
CTCTCCGGTC TGGCAGTTTC CGGCCGAAAA GTGCATGTGG CAGTGGATAT GCTCGAAGGG 
AAAAGACTTC CTTCCGCTCT CGCAGAAAGG TGCAGAAGTG GGTCGGCGCC AGCGATCAAC 
GACGAGACGA CTCATACTTG GCTCCGAGAG GAGTTCGAGA AGGCTGAAAT TATACCAATT 
GACGCGTCGA AACGACGAGT TCGGTTTGAT GATTCTTGTG AGAAGCTGAA AATTATGGAC 
GCGACTGCCA AACAAAACTC ATTGCTCCGC GAGATCGAGG ACCTAAAATC GAAAATTAAA 
GAGCAAGACA CCACATTCGA AGGTCACCGC AATCAAATGT CCGATTTGGA GTCAAAACTG 
CAAGAGCTTC AAGCCAAACA GTCATTCCTC CGGAAATCCG AACCACCTCC AGCCTATGAT 
CGACGGAATG TCACGTATAA CAAACTGTTT CCAGAAAGGG AGAATACGAG TACAACATGT 
GTTATTTTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001293320.1
CDS	1..2952
Translation	MSTVILCHAESVNLDTIRASDANIIRYSVGVKMTELKDMLKQYTAGRIRNLGLHIQTRDTKLRILEDMEVTKESLENAGSFVSQFFRWLISEVLIEGAFIDFLLTTPIENASQICLAVQSVSKKKISVRLVSRFLSSELAPHNRRIVEIYINNEIMITNPNPRATSAQNRYERIRTVGKGAFGSAVLYRRREDSSLVIIKEINMYDLDSSQRRLALNEVSLLSRIEHPNIIAYYDSFEEEGVLMIEMEYADGGTLAQMLSRTQNLLDEEQIGDMMIQMCSAVAYLHENSVLHRDLKTANVFLTRDSFVKIGDFGISKIMGTETLAQGAKTVVGTPYYISPEMCSGVSYNEKSDMWALGCILYEMCCLKKAFEGDNLPALVNSIMTCAYTPVKGPYSAEMKMVIRELLQLDPQKRPSAPQALKMLRPSENRHRHTSGSMRSSVSFTTLYDLHVPTITLSSIPDLPSRISIKQVALSKTHTMVLTNDNELFGFGDNSCGQLGFGKFSKMENGRLVESLKGRDIQSVGVGNYFSVICCDRGTLLTFGTAKNLGLGKDAKDSAKPMLLEQLLRENIKDIYCGHDHVVAVLESGECLGWGSNQYGQLGLPTLEHFFAPTKIPMPSNKKIVYGKAGKDATMLLSDDGSLIAMGSNKHNKLNLTQRVGFFTKEKKTPNDYVNKPTILQGFPERVVDFSVGSYHSGVILESGQVMIFGRNDNTELGLGNSQKMAVRCVKPVKSLLHHACTHVLCGDGFTLVSTTEQELYFWGRKDLTDEDVCIDDLEETVTEKKMKRGTTTEIFDKNVVTLPSLILRLQDERGDKGIRLSGLAVSGRKVHVAVDMLEGKRLPSALAERCRSGSAPAINDETTHTWLREEFEKAEIIPIDASKRRVRFDDSCEKLKIMDATAKQNSLLREIEDLKSKIKEQDTTFEGHRNQMSDLESKLQELQAKQSFLRKSEPPPAYDRRNVTYNKLFPERENTSTTCVIL

Target ORF information:

RefSeq Version	NM_001306391.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans NEK (NEver in mitosis Kinase) Like (nekl-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001306391.1

ORF Insert Sequence:

ATGAGCACGG TGATTCTTTG CCATGCAGAA TCTGTAAATT TGGATACGAT TCGAGCTTCC 
GATGCAAATA TTATTAGATA CAGTGTTGGT GTGAAAATGA CCGAGTTGAA AGACATGCTA 
AAACAATACA CTGCTGGCCG AATTCGGAAC CTCGGCCTCC ATATCCAAAC CCGCGACACG 
AAACTTCGAA TATTAGAGGA TATGGAAGTA ACGAAAGAAT CCCTTGAAAA CGCCGGCTCA 
TTCGTCTCCC AATTTTTTCG ATGGCTCATT TCAGAAGTAT TGATCGAGGG GGCGTTTATC 
GATTTTTTGC TGACCACGCC CATCGAAAAC GCGTCGCAAA TTTGTTTGGC AGTGCAGAGT 
GTTAGCAAGA AAAAGATTTC CGTGCGTTTG GTAAGCAGAT TTTTGTCGTC CGAGCTGGCT 
CCCCATAACA GAAGAATCGT CGAGATTTAT ATCAATAATG AGATAATGAT AACAAACCCG 
AATCCGAGAG CCACATCGGC ACAAAATCGA TATGAACGTA TTCGGACTGT TGGAAAAGGT 
GCCTTCGGGT CCGCCGTGCT CTACCGCCGA AGAGAAGACA GTTCGTTGGT GATTATAAAA 
GAAATCAATA TGTACGATTT GGATAGTTCA CAGAGGAGAT TAGCGCTGAA TGAGGTTTCA 
CTGTTATCAC GTATCGAGCA CCCCAATATA ATTGCATACT ATGATTCATT TGAAGAAGAA 
GGTGTTTTAA TGATTGAAAT GGAATATGCT GATGGTGGAA CACTTGCTCA AATGTTATCA 
AGAACACAGA ATTTGTTGGA TGAAGAGCAA ATTGGCGATA TGATGATTCA AATGTGCTCG 
GCGGTGGCCT ACTTGCATGA GAATAGTGTT TTGCACAGAG ACCTAAAAAC TGCCAACGTC 
TTCCTAACTC GAGACTCATT TGTAAAAATC GGCGACTTTG GAATCTCCAA AATAATGGGC 
ACTGAAACAT TGGCTCAAGG AGCAAAAACA GTTGTCGGAA CACCGTATTA CATAAGTCCA 
GAGATGTGTT CAGGTGTCTC TTATAATGAG AAAAGTGATA TGTGGGCTCT TGGATGTATT 
CTTTATGAAA TGTGTTGTTT GAAGAAAGCG TTTGAAGGTG ACAATCTTCC CGCGTTGGTC 
AATAGTATTA TGACGTGTGC CTACACACCA GTAAAAGGAC CTTACTCGGC TGAAATGAAA 
ATGGTGATAC GGGAATTGCT ACAGTTGGAT CCGCAAAAGA GGCCCTCCGC TCCGCAGGCG 
CTAAAGATGC TGCGACCTTC GGAAAACCGT CACCGACACA CCTCCGGATC AATGAGATCT 
TCGGTTTCGT TCACAACTCT CTACGATTTG CATGTCCCGA CAATCACCCT GTCCTCAATT 
CCAGACCTTC CCAGCCGGAT ATCGATTAAG CAGGTCGCTC TGAGCAAAAC TCATACAATG 
GTGCTCACAA ATGATAATGA ACTTTTCGGA TTTGGTGATA ACTCTTGTGG ACAACTGGGA 
TTCGGGAAGT TTTCGAAAAT GGAAAACGGG CGGTTGGTGG AGAGTTTGAA GGGCAGAGAT 
ATACAATCAG TTGGGGTTGG GAATTATTTT AGTGTAATCT GCTGTGATCG GGGGACCTTA 
TTGACATTTG GTACTGCAAA GAACCTCGGT TTGGGGAAAG ATGCAAAAGA TTCAGCGAAG 
CCGATGCTCC TCGAGCAACT TTTGAGAGAA AATATCAAAG ACATATATTG CGGTCATGAC 
CATGTGGTGG CCGTATTGGA AAGTGGTGAA TGTCTAGGAT GGGGAAGTAA TCAATACGGA 
CAACTCGGGC TGCCAACGTT GGAGCACTTT TTTGCTCCGA CTAAGATTCC GATGCCGAGC 
AACAAGAAAA TCGTGTACGG GAAGGCTGGG AAAGATGCGA CGATGCTATT ATCGGATGAT 
GGAAGTTTGA TAGCAATGGG CTCAAATAAG CATAATAAGC TGAATCTTAC ACAGAGGGTC 
GGGTTCTTTA CAAAAGAGAA AAAGACACCA AACGATTATG TCAACAAGCC GACGATTCTT 
CAAGGATTCC CGGAGCGAGT CGTCGATTTC TCGGTTGGAA GTTATCATTC GGGAGTTATA 
CTGGAATCCG GTCAAGTGAT GATTTTCGGC CGTAATGACA ACACTGAACT CGGCCTCGGG 
AATAGCCAAA AAATGGCGGT TCGATGTGTG AAACCGGTCA AATCGCTGCT TCATCACGCC 
TGCACGCACG TACTCTGCGG TGATGGGTTC ACTCTGGTGA GCACAACGGA GCAGGAACTC 
TACTTTTGGG GACGAAAGGA TCTGACAGAC GAAGATGTGT GTATTGATGA TCTTGAAGAA 
ACCGTCACTG AAAAAAAGAT GAAGCGGGGC ACCACTACTG AGATTTTCGA CAAAAATGTG 
GTCACTTTAC CGTCGTTGAT TCTCAGGCTT CAAGATGAAA GAGGCGACAA AGGGATTCGG 
CTCTCCGGTC TGGCAGTTTC CGGCCGAAAA GTGCATGTGG CAGTGGATAT GCTCGAAGGG 
AAAAGACTTC CTTCCGCTCT CGCAGAAAGG TGCAGAAGTG GGTCGGCGCC AGCGATCAAC 
GACGAGACGA CTCATACTTG GCTCCGAGAG GAGTTCGAGA AGGCTGAAAT TATACCAATT 
GACGCGTCGA AACGACGAGT TCGGTTTGAT GATTCTTGTG AGAAGCTGAA AATTATGGAC 
GCGACTGCCA AACAAAACTC ATTGCTCCGC GAGATCGAGG ACCTAAAATC GAAAATTAAA 
GAGCAAGACA CCACATTCGA AGGTCACCGC AATCAAATGT CCGATTTGGA GTCAAAACTG 
CAAGAGCTTC AAGCCAAACA GTCATTCCTC CGGAAATCCG AACCACCTCC AGCCTATGAT 
CGACGGAATG TCACGTATAA CAAACTGTTT CCAGAAAGGG AGAATACGAG TACAACATGT 
GTTATTTTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nekl-1 ( NM_001306392.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001293321.1 Caenorhabditis elegans NEK (NEver in mitosis Kinase) Like (nekl-1), partial mRNA.

Price & Availability↑

CloneID	OCa336147
Clone ID Related Accession (Same CDS sequence)	NM_001306392.1 , NM_001306392.2
Accession Version	NM_001306392.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2838bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	NEK (NEver in mitosis Kinase) Like
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). COMPLETENESS: incomplete on both ends.

ATGCTAAAAC AATACACTGC TGGCCGAATT CGGAACCTCG GCCTCCATAT CCAAACCCGC 
GACACGAAAC TTCGAATATT AGAGGATATG GAAGTAACGA AAGAATCCCT TGAAAACGCC 
GGCTCATTCG TCTCCCAATT TTTTCGATGG CTCATTTCAG AAGTATTGAT CGAGGGGGCG 
TTTATCGATT TTTTGCTGAC CACGCCCATC GAAAACGCGT CGCAAATTTG TTTGGCAGTG 
CAGAGTGTTA GCAAGAAAAA GATTTCCGTG CGTTTGGTAA GCAGATTTTT GTCGTCCGAG 
CTGGCTCCCC ATAACAGAAG AATCGTCGAG ATTTATATCA ATAATGAGAT AATGATAACA 
AACCCGAATC CGAGAGCCAC ATCGGCACAA AATCGATATG AACGTATTCG GACTGTTGGA 
AAAGGTGCCT TCGGGTCCGC CGTGCTCTAC CGCCGAAGAG AAGACAGTTC GTTGGTGATT 
ATAAAAGAAA TCAATATGTA CGATTTGGAT AGTTCACAGA GGAGATTAGC GCTGAATGAG 
GTTTCACTGT TATCACGTAT CGAGCACCCC AATATAATTG CATACTATGA TTCATTTGAA 
GAAGAAGGTG TTTTAATGAT TGAAATGGAA TATGCTGATG GTGGAACACT TGCTCAAATG 
TTATCAAGAA CACAGAATTT GTTGGATGAA GAGCAAATTG GCGATATGAT GATTCAAATG 
TGCTCGGCGG TGGCCTACTT GCATGAGAAT AGTGTTTTGC ACAGAGACCT AAAAACTGCC 
AACGTCTTCC TAACTCGAGA CTCATTTGTA AAAATCGGCG ACTTTGGAAT CTCCAAAATA 
ATGGGCACTG AAACATTGGC TCAAGGAGCA AAAACAGTTG TCGGAACACC GTATTACATA 
AGTCCAGAGA TGTGTTCAGG TGTCTCTTAT AATGAGAAAA GTGATATGTG GGCTCTTGGA 
TGTATTCTTT ATGAAATGTG TTGTTTGAAG AAAGCGTTTG AAGGTGACAA TCTTCCCGCG 
TTGGTCAATA GTATTATGAC GTGTGCCTAC ACACCAGTAA AAGGACCTTA CTCGGCTGAA 
ATGAAAATGG TGATACGGGA ATTGCTACAG TTGGATCCGC AAAAGAGGCC CTCCGCTCCG 
CAGGCGCTAA AGATGCTGCG ACCTTCGGAA AACCGTCACC GACACACCTC CGGATCAATG 
AGATCTTCGG TTTCGTTCAC AACTCTCTAC GATTTGCATG TCCCGACAAT CACCCTGTCC 
TCAATTCCAG ACCTTCCCAG CCGGATATCG ATTAAGCAGG TCGCTCTGAG CAAAACTCAT 
ACAATGGTGC TCACAAATGA TAATGAACTT TTCGGATTTG GTGATAACTC TTGTGGACAA 
CTGGGATTCG GGAAGTTTTC GAAAATGGAA AACGGGCGGT TGGTGGAGAG TTTGAAGGGC 
AGAGATATAC AATCAGTTGG GGTTGGGAAT TATTTTAGTG TAATCTGCTG TGATCGGGGG 
ACCTTATTGA CATTTGGTAC TGCAAAGAAC CTCGGTTTGG GGAAAGATGC AAAAGATTCA 
GCGAAGCCGA TGCTCCTCGA GCAACTTTTG AGAGAAAATA TCAAAGACAT ATATTGCGGT 
CATGACCATG TGGTGGCCGT ATTGGAAAGT GGTGAATGTC TAGGATGGGG AAGTAATCAA 
TACGGACAAC TCGGGCTGCC AACGTTGGAG CACTTTTTTG CTCCGACTAA GATTCCGATG 
CCGAGCAACA AGAAAATCGT GTACGGGAAG GCTGGGAAAG ATGCGACGAT GCTATTATCG 
GATGATGGAA GTTTGATAGC AATGGGCTCA AATAAGCATA ATAAGCTGAA TCTTACACAG 
AGGGTCGGGT TCTTTACAAA AGAGAAAAAG ACACCAAACG ATTATGTCAA CAAGCCGACG 
ATTCTTCAAG GATTCCCGGA GCGAGTCGTC GATTTCTCGG TTGGAAGTTA TCATTCGGGA 
GTTATACTGG AATCCGGTCA AGTGATGATT TTCGGCCGTA ATGACAACAC TGAACTCGGC 
CTCGGGAATA GCCAAAAAAT GGCGGTTCGA TGTGTGAAAC CGGTCAAATC GCTGCTTCAT 
CACGCCTGCA CGCACGTACT CTGCGGTGAT GGGTTCACTC TGGTGAGCAC AACGGAGCAG 
GAACTCTACT TTTGGGGACG AAAGGATCTG ACAGACGAAG ATGTGTGTAT TGATGATCTT 
GAAGAAACCG TCACTGAAAA AAAGATGAAG CGGGGCACCA CTACTGAGAT TTTCGACAAA 
AATGTGGTCA CTTTACCGTC GTTGATTCTC AGGCTTCAAG ATGAAAGAGG CGACAAAGGG 
ATTCGGCTCT CCGGTCTGGC AGTTTCCGGC CGAAAAGTGC ATGTGGCAGT GGATATGCTC 
GAAGGGAAAA GACTTCCTTC CGCTCTCGCA GAAAGGTGCA GAAGTGGGTC GGCGCCAGCG 
ATCAACGACG AGACGACTCA TACTTGGCTC CGAGAGGAGT TCGAGAAGGC TGAAATTATA 
CCAATTGACG CGTCGAAACG ACGAGTTCGG TTTGATGATT CTTGTGAGAA GCTGAAAATT 
ATGGACGCGA CTGCCAAACA AAACTCATTG CTCCGCGAGA TCGAGGACCT AAAATCGAAA 
ATTAAAGAGC AAGACACCAC ATTCGAAGGT CACCGCAATC AAATGTCCGA TTTGGAGTCA 
AAACTGCAAG AGCTTCAAGC CAAACAGTCA TTCCTCCGGA AATCCGAACC ACCTCCAGCC 
TATGATCGAC GGAATGTCAC GTATAACAAA CTGTTTCCAG AAAGGGAGAA TACGAGTACA 
ACATGTGTTA TTTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001293321.1
CDS	1..2838
Translation	MLKQYTAGRIRNLGLHIQTRDTKLRILEDMEVTKESLENAGSFVSQFFRWLISEVLIEGAFIDFLLTTPIENASQICLAVQSVSKKKISVRLVSRFLSSELAPHNRRIVEIYINNEIMITNPNPRATSAQNRYERIRTVGKGAFGSAVLYRRREDSSLVIIKEINMYDLDSSQRRLALNEVSLLSRIEHPNIIAYYDSFEEEGVLMIEMEYADGGTLAQMLSRTQNLLDEEQIGDMMIQMCSAVAYLHENSVLHRDLKTANVFLTRDSFVKIGDFGISKIMGTETLAQGAKTVVGTPYYISPEMCSGVSYNEKSDMWALGCILYEMCCLKKAFEGDNLPALVNSIMTCAYTPVKGPYSAEMKMVIRELLQLDPQKRPSAPQALKMLRPSENRHRHTSGSMRSSVSFTTLYDLHVPTITLSSIPDLPSRISIKQVALSKTHTMVLTNDNELFGFGDNSCGQLGFGKFSKMENGRLVESLKGRDIQSVGVGNYFSVICCDRGTLLTFGTAKNLGLGKDAKDSAKPMLLEQLLRENIKDIYCGHDHVVAVLESGECLGWGSNQYGQLGLPTLEHFFAPTKIPMPSNKKIVYGKAGKDATMLLSDDGSLIAMGSNKHNKLNLTQRVGFFTKEKKTPNDYVNKPTILQGFPERVVDFSVGSYHSGVILESGQVMIFGRNDNTELGLGNSQKMAVRCVKPVKSLLHHACTHVLCGDGFTLVSTTEQELYFWGRKDLTDEDVCIDDLEETVTEKKMKRGTTTEIFDKNVVTLPSLILRLQDERGDKGIRLSGLAVSGRKVHVAVDMLEGKRLPSALAERCRSGSAPAINDETTHTWLREEFEKAEIIPIDASKRRVRFDDSCEKLKIMDATAKQNSLLREIEDLKSKIKEQDTTFEGHRNQMSDLESKLQELQAKQSFLRKSEPPPAYDRRNVTYNKLFPERENTSTTCVIL

Target ORF information:

RefSeq Version	NM_001306392.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans NEK (NEver in mitosis Kinase) Like (nekl-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001306392.1

ORF Insert Sequence:

ATGCTAAAAC AATACACTGC TGGCCGAATT CGGAACCTCG GCCTCCATAT CCAAACCCGC 
GACACGAAAC TTCGAATATT AGAGGATATG GAAGTAACGA AAGAATCCCT TGAAAACGCC 
GGCTCATTCG TCTCCCAATT TTTTCGATGG CTCATTTCAG AAGTATTGAT CGAGGGGGCG 
TTTATCGATT TTTTGCTGAC CACGCCCATC GAAAACGCGT CGCAAATTTG TTTGGCAGTG 
CAGAGTGTTA GCAAGAAAAA GATTTCCGTG CGTTTGGTAA GCAGATTTTT GTCGTCCGAG 
CTGGCTCCCC ATAACAGAAG AATCGTCGAG ATTTATATCA ATAATGAGAT AATGATAACA 
AACCCGAATC CGAGAGCCAC ATCGGCACAA AATCGATATG AACGTATTCG GACTGTTGGA 
AAAGGTGCCT TCGGGTCCGC CGTGCTCTAC CGCCGAAGAG AAGACAGTTC GTTGGTGATT 
ATAAAAGAAA TCAATATGTA CGATTTGGAT AGTTCACAGA GGAGATTAGC GCTGAATGAG 
GTTTCACTGT TATCACGTAT CGAGCACCCC AATATAATTG CATACTATGA TTCATTTGAA 
GAAGAAGGTG TTTTAATGAT TGAAATGGAA TATGCTGATG GTGGAACACT TGCTCAAATG 
TTATCAAGAA CACAGAATTT GTTGGATGAA GAGCAAATTG GCGATATGAT GATTCAAATG 
TGCTCGGCGG TGGCCTACTT GCATGAGAAT AGTGTTTTGC ACAGAGACCT AAAAACTGCC 
AACGTCTTCC TAACTCGAGA CTCATTTGTA AAAATCGGCG ACTTTGGAAT CTCCAAAATA 
ATGGGCACTG AAACATTGGC TCAAGGAGCA AAAACAGTTG TCGGAACACC GTATTACATA 
AGTCCAGAGA TGTGTTCAGG TGTCTCTTAT AATGAGAAAA GTGATATGTG GGCTCTTGGA 
TGTATTCTTT ATGAAATGTG TTGTTTGAAG AAAGCGTTTG AAGGTGACAA TCTTCCCGCG 
TTGGTCAATA GTATTATGAC GTGTGCCTAC ACACCAGTAA AAGGACCTTA CTCGGCTGAA 
ATGAAAATGG TGATACGGGA ATTGCTACAG TTGGATCCGC AAAAGAGGCC CTCCGCTCCG 
CAGGCGCTAA AGATGCTGCG ACCTTCGGAA AACCGTCACC GACACACCTC CGGATCAATG 
AGATCTTCGG TTTCGTTCAC AACTCTCTAC GATTTGCATG TCCCGACAAT CACCCTGTCC 
TCAATTCCAG ACCTTCCCAG CCGGATATCG ATTAAGCAGG TCGCTCTGAG CAAAACTCAT 
ACAATGGTGC TCACAAATGA TAATGAACTT TTCGGATTTG GTGATAACTC TTGTGGACAA 
CTGGGATTCG GGAAGTTTTC GAAAATGGAA AACGGGCGGT TGGTGGAGAG TTTGAAGGGC 
AGAGATATAC AATCAGTTGG GGTTGGGAAT TATTTTAGTG TAATCTGCTG TGATCGGGGG 
ACCTTATTGA CATTTGGTAC TGCAAAGAAC CTCGGTTTGG GGAAAGATGC AAAAGATTCA 
GCGAAGCCGA TGCTCCTCGA GCAACTTTTG AGAGAAAATA TCAAAGACAT ATATTGCGGT 
CATGACCATG TGGTGGCCGT ATTGGAAAGT GGTGAATGTC TAGGATGGGG AAGTAATCAA 
TACGGACAAC TCGGGCTGCC AACGTTGGAG CACTTTTTTG CTCCGACTAA GATTCCGATG 
CCGAGCAACA AGAAAATCGT GTACGGGAAG GCTGGGAAAG ATGCGACGAT GCTATTATCG 
GATGATGGAA GTTTGATAGC AATGGGCTCA AATAAGCATA ATAAGCTGAA TCTTACACAG 
AGGGTCGGGT TCTTTACAAA AGAGAAAAAG ACACCAAACG ATTATGTCAA CAAGCCGACG 
ATTCTTCAAG GATTCCCGGA GCGAGTCGTC GATTTCTCGG TTGGAAGTTA TCATTCGGGA 
GTTATACTGG AATCCGGTCA AGTGATGATT TTCGGCCGTA ATGACAACAC TGAACTCGGC 
CTCGGGAATA GCCAAAAAAT GGCGGTTCGA TGTGTGAAAC CGGTCAAATC GCTGCTTCAT 
CACGCCTGCA CGCACGTACT CTGCGGTGAT GGGTTCACTC TGGTGAGCAC AACGGAGCAG 
GAACTCTACT TTTGGGGACG AAAGGATCTG ACAGACGAAG ATGTGTGTAT TGATGATCTT 
GAAGAAACCG TCACTGAAAA AAAGATGAAG CGGGGCACCA CTACTGAGAT TTTCGACAAA 
AATGTGGTCA CTTTACCGTC GTTGATTCTC AGGCTTCAAG ATGAAAGAGG CGACAAAGGG 
ATTCGGCTCT CCGGTCTGGC AGTTTCCGGC CGAAAAGTGC ATGTGGCAGT GGATATGCTC 
GAAGGGAAAA GACTTCCTTC CGCTCTCGCA GAAAGGTGCA GAAGTGGGTC GGCGCCAGCG 
ATCAACGACG AGACGACTCA TACTTGGCTC CGAGAGGAGT TCGAGAAGGC TGAAATTATA 
CCAATTGACG CGTCGAAACG ACGAGTTCGG TTTGATGATT CTTGTGAGAA GCTGAAAATT 
ATGGACGCGA CTGCCAAACA AAACTCATTG CTCCGCGAGA TCGAGGACCT AAAATCGAAA 
ATTAAAGAGC AAGACACCAC ATTCGAAGGT CACCGCAATC AAATGTCCGA TTTGGAGTCA 
AAACTGCAAG AGCTTCAAGC CAAACAGTCA TTCCTCCGGA AATCCGAACC ACCTCCAGCC 
TATGATCGAC GGAATGTCAC GTATAACAAA CTGTTTCCAG AAAGGGAGAA TACGAGTACA 
ACATGTGTTA TTTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nekl-1 ( NM_001306392.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001293321.1 Caenorhabditis elegans Protein kinase domain-containing protein (nekl-1), partial mRNA.

Price & Availability↑

CloneID	OCa336147
Clone ID Related Accession (Same CDS sequence)	NM_001306392.1 , NM_001306392.2
Accession Version	NM_001306392.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2838bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Protein kinase domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_001306392.1. COMPLETENESS: incomplete on the 5' end.

ATGCTAAAAC AATACACTGC TGGCCGAATT CGGAACCTCG GCCTCCATAT CCAAACCCGC 
GACACGAAAC TTCGAATATT AGAGGATATG GAAGTAACGA AAGAATCCCT TGAAAACGCC 
GGCTCATTCG TCTCCCAATT TTTTCGATGG CTCATTTCAG AAGTATTGAT CGAGGGGGCG 
TTTATCGATT TTTTGCTGAC CACGCCCATC GAAAACGCGT CGCAAATTTG TTTGGCAGTG 
CAGAGTGTTA GCAAGAAAAA GATTTCCGTG CGTTTGGTAA GCAGATTTTT GTCGTCCGAG 
CTGGCTCCCC ATAACAGAAG AATCGTCGAG ATTTATATCA ATAATGAGAT AATGATAACA 
AACCCGAATC CGAGAGCCAC ATCGGCACAA AATCGATATG AACGTATTCG GACTGTTGGA 
AAAGGTGCCT TCGGGTCCGC CGTGCTCTAC CGCCGAAGAG AAGACAGTTC GTTGGTGATT 
ATAAAAGAAA TCAATATGTA CGATTTGGAT AGTTCACAGA GGAGATTAGC GCTGAATGAG 
GTTTCACTGT TATCACGTAT CGAGCACCCC AATATAATTG CATACTATGA TTCATTTGAA 
GAAGAAGGTG TTTTAATGAT TGAAATGGAA TATGCTGATG GTGGAACACT TGCTCAAATG 
TTATCAAGAA CACAGAATTT GTTGGATGAA GAGCAAATTG GCGATATGAT GATTCAAATG 
TGCTCGGCGG TGGCCTACTT GCATGAGAAT AGTGTTTTGC ACAGAGACCT AAAAACTGCC 
AACGTCTTCC TAACTCGAGA CTCATTTGTA AAAATCGGCG ACTTTGGAAT CTCCAAAATA 
ATGGGCACTG AAACATTGGC TCAAGGAGCA AAAACAGTTG TCGGAACACC GTATTACATA 
AGTCCAGAGA TGTGTTCAGG TGTCTCTTAT AATGAGAAAA GTGATATGTG GGCTCTTGGA 
TGTATTCTTT ATGAAATGTG TTGTTTGAAG AAAGCGTTTG AAGGTGACAA TCTTCCCGCG 
TTGGTCAATA GTATTATGAC GTGTGCCTAC ACACCAGTAA AAGGACCTTA CTCGGCTGAA 
ATGAAAATGG TGATACGGGA ATTGCTACAG TTGGATCCGC AAAAGAGGCC CTCCGCTCCG 
CAGGCGCTAA AGATGCTGCG ACCTTCGGAA AACCGTCACC GACACACCTC CGGATCAATG 
AGATCTTCGG TTTCGTTCAC AACTCTCTAC GATTTGCATG TCCCGACAAT CACCCTGTCC 
TCAATTCCAG ACCTTCCCAG CCGGATATCG ATTAAGCAGG TCGCTCTGAG CAAAACTCAT 
ACAATGGTGC TCACAAATGA TAATGAACTT TTCGGATTTG GTGATAACTC TTGTGGACAA 
CTGGGATTCG GGAAGTTTTC GAAAATGGAA AACGGGCGGT TGGTGGAGAG TTTGAAGGGC 
AGAGATATAC AATCAGTTGG GGTTGGGAAT TATTTTAGTG TAATCTGCTG TGATCGGGGG 
ACCTTATTGA CATTTGGTAC TGCAAAGAAC CTCGGTTTGG GGAAAGATGC AAAAGATTCA 
GCGAAGCCGA TGCTCCTCGA GCAACTTTTG AGAGAAAATA TCAAAGACAT ATATTGCGGT 
CATGACCATG TGGTGGCCGT ATTGGAAAGT GGTGAATGTC TAGGATGGGG AAGTAATCAA 
TACGGACAAC TCGGGCTGCC AACGTTGGAG CACTTTTTTG CTCCGACTAA GATTCCGATG 
CCGAGCAACA AGAAAATCGT GTACGGGAAG GCTGGGAAAG ATGCGACGAT GCTATTATCG 
GATGATGGAA GTTTGATAGC AATGGGCTCA AATAAGCATA ATAAGCTGAA TCTTACACAG 
AGGGTCGGGT TCTTTACAAA AGAGAAAAAG ACACCAAACG ATTATGTCAA CAAGCCGACG 
ATTCTTCAAG GATTCCCGGA GCGAGTCGTC GATTTCTCGG TTGGAAGTTA TCATTCGGGA 
GTTATACTGG AATCCGGTCA AGTGATGATT TTCGGCCGTA ATGACAACAC TGAACTCGGC 
CTCGGGAATA GCCAAAAAAT GGCGGTTCGA TGTGTGAAAC CGGTCAAATC GCTGCTTCAT 
CACGCCTGCA CGCACGTACT CTGCGGTGAT GGGTTCACTC TGGTGAGCAC AACGGAGCAG 
GAACTCTACT TTTGGGGACG AAAGGATCTG ACAGACGAAG ATGTGTGTAT TGATGATCTT 
GAAGAAACCG TCACTGAAAA AAAGATGAAG CGGGGCACCA CTACTGAGAT TTTCGACAAA 
AATGTGGTCA CTTTACCGTC GTTGATTCTC AGGCTTCAAG ATGAAAGAGG CGACAAAGGG 
ATTCGGCTCT CCGGTCTGGC AGTTTCCGGC CGAAAAGTGC ATGTGGCAGT GGATATGCTC 
GAAGGGAAAA GACTTCCTTC CGCTCTCGCA GAAAGGTGCA GAAGTGGGTC GGCGCCAGCG 
ATCAACGACG AGACGACTCA TACTTGGCTC CGAGAGGAGT TCGAGAAGGC TGAAATTATA 
CCAATTGACG CGTCGAAACG ACGAGTTCGG TTTGATGATT CTTGTGAGAA GCTGAAAATT 
ATGGACGCGA CTGCCAAACA AAACTCATTG CTCCGCGAGA TCGAGGACCT AAAATCGAAA 
ATTAAAGAGC AAGACACCAC ATTCGAAGGT CACCGCAATC AAATGTCCGA TTTGGAGTCA 
AAACTGCAAG AGCTTCAAGC CAAACAGTCA TTCCTCCGGA AATCCGAACC ACCTCCAGCC 
TATGATCGAC GGAATGTCAC GTATAACAAA CTGTTTCCAG AAAGGGAGAA TACGAGTACA 
ACATGTGTTA TTTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001293321.1
CDS	1..2838
Translation	MLKQYTAGRIRNLGLHIQTRDTKLRILEDMEVTKESLENAGSFVSQFFRWLISEVLIEGAFIDFLLTTPIENASQICLAVQSVSKKKISVRLVSRFLSSELAPHNRRIVEIYINNEIMITNPNPRATSAQNRYERIRTVGKGAFGSAVLYRRREDSSLVIIKEINMYDLDSSQRRLALNEVSLLSRIEHPNIIAYYDSFEEEGVLMIEMEYADGGTLAQMLSRTQNLLDEEQIGDMMIQMCSAVAYLHENSVLHRDLKTANVFLTRDSFVKIGDFGISKIMGTETLAQGAKTVVGTPYYISPEMCSGVSYNEKSDMWALGCILYEMCCLKKAFEGDNLPALVNSIMTCAYTPVKGPYSAEMKMVIRELLQLDPQKRPSAPQALKMLRPSENRHRHTSGSMRSSVSFTTLYDLHVPTITLSSIPDLPSRISIKQVALSKTHTMVLTNDNELFGFGDNSCGQLGFGKFSKMENGRLVESLKGRDIQSVGVGNYFSVICCDRGTLLTFGTAKNLGLGKDAKDSAKPMLLEQLLRENIKDIYCGHDHVVAVLESGECLGWGSNQYGQLGLPTLEHFFAPTKIPMPSNKKIVYGKAGKDATMLLSDDGSLIAMGSNKHNKLNLTQRVGFFTKEKKTPNDYVNKPTILQGFPERVVDFSVGSYHSGVILESGQVMIFGRNDNTELGLGNSQKMAVRCVKPVKSLLHHACTHVLCGDGFTLVSTTEQELYFWGRKDLTDEDVCIDDLEETVTEKKMKRGTTTEIFDKNVVTLPSLILRLQDERGDKGIRLSGLAVSGRKVHVAVDMLEGKRLPSALAERCRSGSAPAINDETTHTWLREEFEKAEIIPIDASKRRVRFDDSCEKLKIMDATAKQNSLLREIEDLKSKIKEQDTTFEGHRNQMSDLESKLQELQAKQSFLRKSEPPPAYDRRNVTYNKLFPERENTSTTCVIL

Target ORF information:

RefSeq Version	NM_001306392.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Protein kinase domain-containing protein (nekl-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001306392.2

ORF Insert Sequence:

ATGCTAAAAC AATACACTGC TGGCCGAATT CGGAACCTCG GCCTCCATAT CCAAACCCGC 
GACACGAAAC TTCGAATATT AGAGGATATG GAAGTAACGA AAGAATCCCT TGAAAACGCC 
GGCTCATTCG TCTCCCAATT TTTTCGATGG CTCATTTCAG AAGTATTGAT CGAGGGGGCG 
TTTATCGATT TTTTGCTGAC CACGCCCATC GAAAACGCGT CGCAAATTTG TTTGGCAGTG 
CAGAGTGTTA GCAAGAAAAA GATTTCCGTG CGTTTGGTAA GCAGATTTTT GTCGTCCGAG 
CTGGCTCCCC ATAACAGAAG AATCGTCGAG ATTTATATCA ATAATGAGAT AATGATAACA 
AACCCGAATC CGAGAGCCAC ATCGGCACAA AATCGATATG AACGTATTCG GACTGTTGGA 
AAAGGTGCCT TCGGGTCCGC CGTGCTCTAC CGCCGAAGAG AAGACAGTTC GTTGGTGATT 
ATAAAAGAAA TCAATATGTA CGATTTGGAT AGTTCACAGA GGAGATTAGC GCTGAATGAG 
GTTTCACTGT TATCACGTAT CGAGCACCCC AATATAATTG CATACTATGA TTCATTTGAA 
GAAGAAGGTG TTTTAATGAT TGAAATGGAA TATGCTGATG GTGGAACACT TGCTCAAATG 
TTATCAAGAA CACAGAATTT GTTGGATGAA GAGCAAATTG GCGATATGAT GATTCAAATG 
TGCTCGGCGG TGGCCTACTT GCATGAGAAT AGTGTTTTGC ACAGAGACCT AAAAACTGCC 
AACGTCTTCC TAACTCGAGA CTCATTTGTA AAAATCGGCG ACTTTGGAAT CTCCAAAATA 
ATGGGCACTG AAACATTGGC TCAAGGAGCA AAAACAGTTG TCGGAACACC GTATTACATA 
AGTCCAGAGA TGTGTTCAGG TGTCTCTTAT AATGAGAAAA GTGATATGTG GGCTCTTGGA 
TGTATTCTTT ATGAAATGTG TTGTTTGAAG AAAGCGTTTG AAGGTGACAA TCTTCCCGCG 
TTGGTCAATA GTATTATGAC GTGTGCCTAC ACACCAGTAA AAGGACCTTA CTCGGCTGAA 
ATGAAAATGG TGATACGGGA ATTGCTACAG TTGGATCCGC AAAAGAGGCC CTCCGCTCCG 
CAGGCGCTAA AGATGCTGCG ACCTTCGGAA AACCGTCACC GACACACCTC CGGATCAATG 
AGATCTTCGG TTTCGTTCAC AACTCTCTAC GATTTGCATG TCCCGACAAT CACCCTGTCC 
TCAATTCCAG ACCTTCCCAG CCGGATATCG ATTAAGCAGG TCGCTCTGAG CAAAACTCAT 
ACAATGGTGC TCACAAATGA TAATGAACTT TTCGGATTTG GTGATAACTC TTGTGGACAA 
CTGGGATTCG GGAAGTTTTC GAAAATGGAA AACGGGCGGT TGGTGGAGAG TTTGAAGGGC 
AGAGATATAC AATCAGTTGG GGTTGGGAAT TATTTTAGTG TAATCTGCTG TGATCGGGGG 
ACCTTATTGA CATTTGGTAC TGCAAAGAAC CTCGGTTTGG GGAAAGATGC AAAAGATTCA 
GCGAAGCCGA TGCTCCTCGA GCAACTTTTG AGAGAAAATA TCAAAGACAT ATATTGCGGT 
CATGACCATG TGGTGGCCGT ATTGGAAAGT GGTGAATGTC TAGGATGGGG AAGTAATCAA 
TACGGACAAC TCGGGCTGCC AACGTTGGAG CACTTTTTTG CTCCGACTAA GATTCCGATG 
CCGAGCAACA AGAAAATCGT GTACGGGAAG GCTGGGAAAG ATGCGACGAT GCTATTATCG 
GATGATGGAA GTTTGATAGC AATGGGCTCA AATAAGCATA ATAAGCTGAA TCTTACACAG 
AGGGTCGGGT TCTTTACAAA AGAGAAAAAG ACACCAAACG ATTATGTCAA CAAGCCGACG 
ATTCTTCAAG GATTCCCGGA GCGAGTCGTC GATTTCTCGG TTGGAAGTTA TCATTCGGGA 
GTTATACTGG AATCCGGTCA AGTGATGATT TTCGGCCGTA ATGACAACAC TGAACTCGGC 
CTCGGGAATA GCCAAAAAAT GGCGGTTCGA TGTGTGAAAC CGGTCAAATC GCTGCTTCAT 
CACGCCTGCA CGCACGTACT CTGCGGTGAT GGGTTCACTC TGGTGAGCAC AACGGAGCAG 
GAACTCTACT TTTGGGGACG AAAGGATCTG ACAGACGAAG ATGTGTGTAT TGATGATCTT 
GAAGAAACCG TCACTGAAAA AAAGATGAAG CGGGGCACCA CTACTGAGAT TTTCGACAAA 
AATGTGGTCA CTTTACCGTC GTTGATTCTC AGGCTTCAAG ATGAAAGAGG CGACAAAGGG 
ATTCGGCTCT CCGGTCTGGC AGTTTCCGGC CGAAAAGTGC ATGTGGCAGT GGATATGCTC 
GAAGGGAAAA GACTTCCTTC CGCTCTCGCA GAAAGGTGCA GAAGTGGGTC GGCGCCAGCG 
ATCAACGACG AGACGACTCA TACTTGGCTC CGAGAGGAGT TCGAGAAGGC TGAAATTATA 
CCAATTGACG CGTCGAAACG ACGAGTTCGG TTTGATGATT CTTGTGAGAA GCTGAAAATT 
ATGGACGCGA CTGCCAAACA AAACTCATTG CTCCGCGAGA TCGAGGACCT AAAATCGAAA 
ATTAAAGAGC AAGACACCAC ATTCGAAGGT CACCGCAATC AAATGTCCGA TTTGGAGTCA 
AAACTGCAAG AGCTTCAAGC CAAACAGTCA TTCCTCCGGA AATCCGAACC ACCTCCAGCC 
TATGATCGAC GGAATGTCAC GTATAACAAA CTGTTTCCAG AAAGGGAGAA TACGAGTACA 
ACATGTGTTA TTTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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nekl-1 cDNA ORF clone, Caenorhabditis elegans

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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