NEFH gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_005218112.2	XP_005218169.1	neurofilament heavy polypeptide isoform X1
XM_010814137.1	XP_010812439.1	neurofilament heavy polypeptide isoform X4
XM_010823482.2	XP_010821784.2	neurofilament heavy polypeptide isoform X9
XM_024977820.1	XP_024833588.1	neurofilament heavy polypeptide isoform X5
XM_005218121.3	XP_005218178.1	neurofilament heavy polypeptide isoform X6
XM_005218119.2	XP_005218176.1	neurofilament heavy polypeptide isoform X5
XM_010814141.1	XP_010812443.1	neurofilament heavy polypeptide isoform X8
XM_010823474.2	XP_010821776.2	neurofilament heavy polypeptide isoform X2
XM_010823475.2	XP_010821777.2	neurofilament heavy polypeptide isoform X3
XM_010823480.2	XP_010821782.2	neurofilament heavy polypeptide isoform X7
XM_005218114.3	XP_005218171.1	neurofilament heavy polypeptide isoform X3
XM_005218115.3	XP_005218172.1	neurofilament heavy polypeptide isoform X4
XM_005218114.2	XP_005218171.1	neurofilament heavy polypeptide isoform X2
XM_005218115.2	XP_005218172.1	neurofilament heavy polypeptide isoform X3
XM_010823477.2	XP_010821779.2	neurofilament heavy polypeptide isoform X5
XM_010823481.2	XP_010821783.2	neurofilament heavy polypeptide isoform X8
XM_005218112.3	XP_005218169.1	neurofilament heavy polypeptide isoform X1
XM_024977819.1	XP_024833587.1	neurofilament heavy polypeptide isoform X2
XM_024977821.1	XP_024833589.1	neurofilament heavy polypeptide isoform X7
XM_010814139.1	XP_010812441.1	neurofilament heavy polypeptide isoform X7
XM_010814142.1	XP_010812444.1	neurofilament heavy polypeptide isoform X9
XM_005195129.3	XP_005195186.2	neurofilament heavy polypeptide isoform X1
XM_010823476.2	XP_010821778.2	neurofilament heavy polypeptide isoform X4
XM_010823479.2	XP_010821781.2	neurofilament heavy polypeptide isoform X6
XM_005218121.2	XP_005218178.1	neurofilament heavy polypeptide isoform X6

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa224691	XM_024977819.1 Latest version!	Bos taurus neurofilament heavy (NEFH), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa224692	XM_024977820.1 Latest version!	Bos taurus neurofilament heavy (NEFH), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa224693	XM_024977821.1 Latest version!	Bos taurus neurofilament heavy (NEFH), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa28068	XM_005195129.3 Latest version!	Bos taurus neurofilament, heavy polypeptide (NEFH), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa28068	XM_005218112.3 Latest version!	Bos taurus neurofilament heavy (NEFH), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa28070	XM_005218114.3 Latest version!	Bos taurus neurofilament heavy (NEFH), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa28070	XM_010823474.2 Latest version!	Bos taurus neurofilament, heavy polypeptide (NEFH), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa28071	XM_005218115.3 Latest version!	Bos taurus neurofilament heavy (NEFH), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa28071	XM_010823475.2 Latest version!	Bos taurus neurofilament, heavy polypeptide (NEFH), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa28073	XM_010823476.2 Latest version!	Bos taurus neurofilament, heavy polypeptide (NEFH), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa28073	XM_010814137.1 Latest version!	Bos taurus neurofilament, heavy polypeptide (NEFH), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa28075	XM_010823477.2 Latest version!	Bos taurus neurofilament, heavy polypeptide (NEFH), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa28075	XM_005218119.2 Latest version!	Bos taurus neurofilament, heavy polypeptide (NEFH), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa28077	XM_010823479.2 Latest version!	Bos taurus neurofilament, heavy polypeptide (NEFH), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa28077	XM_005218121.3 Latest version!	Bos taurus neurofilament heavy (NEFH), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa51617	XM_010823480.2 Latest version!	Bos taurus neurofilament, heavy polypeptide (NEFH), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa51617	XM_010814139.1 Latest version!	Bos taurus neurofilament, heavy polypeptide (NEFH), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa51618	XM_010823481.2 Latest version!	Bos taurus neurofilament, heavy polypeptide (NEFH), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OBa51618	XM_010814141.1 Latest version!	Bos taurus neurofilament, heavy polypeptide (NEFH), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OBa51619	XM_010823482.2 Latest version!	Bos taurus neurofilament, heavy polypeptide (NEFH), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OBa51619	XM_010814142.1 Latest version!	Bos taurus neurofilament, heavy polypeptide (NEFH), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
View Old Accession Versions >>
OBa28070	XM_005218114.2	Bos taurus neurofilament, heavy polypeptide (NEFH), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa28071	XM_005218115.2	Bos taurus neurofilament, heavy polypeptide (NEFH), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa28068	XM_005218112.2	Bos taurus neurofilament, heavy polypeptide (NEFH), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa28077	XM_005218121.2	Bos taurus neurofilament, heavy polypeptide (NEFH), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
Hide Old Accession Versions >>

NEFH ( XM_024977819.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024833587.1 Bos taurus neurofilament heavy (NEFH), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa224691
Clone ID Related Accession (Same CDS sequence)	XM_024977819.1
Accession Version	XM_024977819.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	neurofilament heavy polypeptide isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCA GCGGCGCCGA CGCGCTGCTG GGCGCCTTCG CGCCGCTCCA CGGAGGCGGC 
AGCTTGCACT ATGCACTGGC TCGCAAAGGC GGCGCCGGCG GAGCGCGCTC TGCAGCCGGC 
TCCTCCAGCG GATTCCACTC ATGGGCGCGG ACCTCCGTGA GCTCCGTGTC GGCCTCCCCG 
AGCCGCTTCC GAGGCGCCGG GACTACCTCC AGCACCGACT CGCTAGACAC CCTGAGCAAC 
GGACCGGAGG GCTGCGTGGT GGTGGCGCGC AGTGAGAAGG AGCAGCTGCA GGTGCTGAAC 
GACCGCTTCG CGGGCTACAT CGACAAGGTG CGGCAGCTCG AGGCGCATAA CCGCAGCCTG 
GAGGGCGAGG CGGCGGCGCT GCGGCAGCAG CAGGCGGGCC GCGCAGCCAT GGGCGAGCTG 
TACGAGCGCG AGGTGCGCGA GATGCGCGGC GCAGTGCTGC GCTTGGGCGC GGCTCGCGGC 
CAGCTGCGCC TGGAGCAGGA GCACCTGCTG GAGGACATCG CGCACGTGCG CCAGCGCCTG 
GACGACGAGG CCCGGCAGCG CGAGGAGGCC GAGGCAGCGA CGCGCGCACT GGCCCGCTTT 
GCGCAGGAGG CCGAGGCGGC GCGCGTCGAG CTGCAGAAGA AGGCACAAGC CCTGCAGGAA 
GAGTGCGGCT ACCTGCGGCG TCACCACCAG GAGGAGGTGG GCGAGCTGCT CGGCCAGATC 
CAGAGCAGCA GCGCCGCGCA GACGCAGGCC GAGGCGCGCG ATGCCCTCAA GTGCGACGTG 
ACGTCGGCCC TGCGCGAGAT CCGCGCGCAG CTTGAAGGCC ACACGGTGCA GAGCACGCTT 
CAGTCCGAGG AGTGGTTCCG AGTGAGGCTG GACCGACTGT CCGAGGCAGC CAAGGTGAAC 
ACAGACGCCA TGCGCTCAGC ACAGGAGGAG ATATCTGAGT ACCGGCGCCA GCTGCAGGCC 
AGGACCACAG AGCTGGAGAC GCTCAAAAGC ACCAAGGACT CACTGGAGAG GCAGCGCTCT 
GAGCTGGAGG ACCGTCACCA GGCTGACATC GCATCCTACC AGGAGGCCAT CCAGCAGCTG 
GATGCAGAGC TGAGGAACAC CAAGTGGGAG ATGGCAGCCC AGCTCCGAGA GTACCAGGAT 
CTACTCAATG TCAAGATGGC TCTGGACATT GAGATCGCTG CTTACAGAAA ACTCCTGGAG 
GGTGAAGAAT GTCGAATTGG CTTTGGCCCG AGTCCTTTCC TCCTTCCAGA AGGACTCCCC 
AAGATCCCCT CCGTGTCCAC TCACATCAAA GTCAAAAGTG AAGAGAAGAT CAAGATGGTA 
GAAAAGTCAG AGAAGGAAAC TGTGATTGTG GAGGAACAGA CAGAGGAGGT CCAAGTGACT 
GAAGAAGTGA CTGAAGAAGA AGAGAAAGAG GCCAAAGAGG AGGAAGGAGA AGCAGTAAAG 
TCTCCCCCAG CAGAAGAGGC TGCATCCCCA GAGAAGGAGG AGGCCAAGTC ACCAGCCGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAGG AGGCCAAGTC CCCACAGAAG 
GAAGAGGCCA AGTCACCAGC TGAGGTGAAG TCCCCTGAGA AGGCCAAGTC CCCCGTGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAAG AAGCCAAATC CCCAGAGAAG 
GCCAAGTCCC CAGTGGAGGC CAAGTCTCCA GAAAAGGCCA AGTCCCCCAT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGT CAAATCCCCA GTGGAGGCCA AATCCCCAGA GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GAGAAAGCCA AGTCCCCTGT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GTGGAAGCCA AGTCCCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT GTGAAGGAGG AGGCCAAGTC CCCAGAGAAG 
GCCAAGTCTC CTGTGAAGGA GGAGGCCAAG TCCCCAGAGA AGGCCAAGTC TCCTGTGAAA 
GAGGAGGCCA AGTCCCCAGA GAAGGCCAAG TCTCCTGTGA AGGAGGAGGC CAAGTCGCCA 
GAGAAGGCCA AGTCTCCTGT GAAAGAGGAG GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT 
GTGAAAGAGG AGGCCAAGTC CCCAGAGAAG GCCAAGTCCC CAGTAGAGGC CAAGTCCCCA 
GAGAAGGCCA AGTCTCCTGC GAAGGAGGAG GCCAAGTCGC CAGAGAAGGC CAAGTCTCCT 
GTGAAAGAGG AGGCCAAGTC CCCAGAGAAG GCCAAGTCTC CTGTGAAAGA GGAGGCCAAG 
TCCCCAGAGA AGGCCAAGTC TCCTGCGAAG GAGGAGGCCA AGTCCCCAGA GAAGGCCAAG 
TCCCCAGAGA AGCCCAAAAG CCCTGTGAAG GAGGAGGCCA AGTCCCCAGA GAAGCCCAAA 
AGCCCTGTGA AGGAGGAGGC CAAGTCCCCA GAGAAGCCCA AAAGCCCTGT CAAGGAGGAG 
GCCAAATCCC CAGAGAAGGC CAAGTCTCCT GTGAAGGATG AGGCCAAGGC TCCTGGGAAG 
GAGGTCCCAA AGAAGGAGGA GGCCAAGTCC CCTGTGAAGG AGGAAGAAAA ACCCCAGGAG 
GTGAGAGCCA AAGAGCCCCC AAAGAAGGCA GAGGAAGAGA AAGCTCCAGC CATACCCAAA 
GCTGAGGAGA AGAAGGACAG CAAGAAAGAT GAGGTGCCCA AGAAGGAGGC TCCAAAGCCC 
GAGGAGAAGA AGGAGCCTGC TGTGGAAAAG CCCAAAGAAC CCAAAGCTGA AGCCAAGAAG 
GAAGAGGCTG AAGATAAGAA AAAAGCAACC ACCCCAGAGA AGGAGGCTCC TGCCAAGGGG 
AAGGAAGAGG CCAAGCCTAA AGAGAAGACT GAGGTGGCCA AGAAGGAACC AGATGATGCC 
AAGCCCAAAG AACCCAGCAA AGCAGCAGAA AAGCCAAAGA AGGAAGAGAC GCCGGCAGCA 
CCGGAGAAAA AAGACGCCAA GGAGGGGAAG GCCGCAGAAG CCAAGAAGCC TGAGGAGAAA 
CCCAAAACAG AGGCCCAAGC CAAGGAACCT GGCAAGGAGG CCTCCACACC CGGGAAAGCC 
AAGGCTGAGA AATCCTCCAG CACAGACCAG AAAGACAGCA GACCTGCAGA GAAGGCCGCA 
GAAGATAAGG CCACCAAGGG AGAGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024833587.1
CDS	76..3285
Translation	MSFSGADALLGAFAPLHGGGSLHYALARKGGAGGARSAAGSSSGFHSWARTSVSSVSASPSRFRGAGTTSSTDSLDTLSNGPEGCVVVARSEKEQLQVLNDRFAGYIDKVRQLEAHNRSLEGEAAALRQQQAGRAAMGELYEREVREMRGAVLRLGAARGQLRLEQEHLLEDIAHVRQRLDDEARQREEAEAATRALARFAQEAEAARVELQKKAQALQEECGYLRRHHQEEVGELLGQIQSSSAAQTQAEARDALKCDVTSALREIRAQLEGHTVQSTLQSEEWFRVRLDRLSEAAKVNTDAMRSAQEEISEYRRQLQARTTELETLKSTKDSLERQRSELEDRHQADIASYQEAIQQLDAELRNTKWEMAAQLREYQDLLNVKMALDIEIAAYRKLLEGEECRIGFGPSPFLLPEGLPKIPSVSTHIKVKSEEKIKMVEKSEKETVIVEEQTEEVQVTEEVTEEEEKEAKEEEGEAVKSPPAEEAASPEKEEAKSPAEAKSPEKAKSPVKEEAKSPQKEEAKSPAEVKSPEKAKSPVEAKSPEKAKSPVKEEAKSPEKAKSPVEAKSPEKAKSPMKEEAKSPEKVKSPVEAKSPEKEEAKSPEKAKSPEKAKSPVKEEAKSPEKAKSPVEAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVEAKSPEKAKSPAKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPAKEEAKSPEKAKSPEKPKSPVKEEAKSPEKPKSPVKEEAKSPEKPKSPVKEEAKSPEKAKSPVKDEAKAPGKEVPKKEEAKSPVKEEEKPQEVRAKEPPKKAEEEKAPAIPKAEEKKDSKKDEVPKKEAPKPEEKKEPAVEKPKEPKAEAKKEEAEDKKKATTPEKEAPAKGKEEAKPKEKTEVAKKEPDDAKPKEPSKAAEKPKKEETPAAPEKKDAKEGKAAEAKKPEEKPKTEAQAKEPGKEASTPGKAKAEKSSSTDQKDSRPAEKAAEDKATKGEK

Target ORF information:

RefSeq Version	XM_024977819.1
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofilament heavy (NEFH), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024977819.1

ORF Insert Sequence:

ATGAGCTTCA GCGGCGCCGA CGCGCTGCTG GGCGCCTTCG CGCCGCTCCA CGGAGGCGGC 
AGCTTGCACT ATGCACTGGC TCGCAAAGGC GGCGCCGGCG GAGCGCGCTC TGCAGCCGGC 
TCCTCCAGCG GATTCCACTC ATGGGCGCGG ACCTCCGTGA GCTCCGTGTC GGCCTCCCCG 
AGCCGCTTCC GAGGCGCCGG GACTACCTCC AGCACCGACT CGCTAGACAC CCTGAGCAAC 
GGACCGGAGG GCTGCGTGGT GGTGGCGCGC AGTGAGAAGG AGCAGCTGCA GGTGCTGAAC 
GACCGCTTCG CGGGCTACAT CGACAAGGTG CGGCAGCTCG AGGCGCATAA CCGCAGCCTG 
GAGGGCGAGG CGGCGGCGCT GCGGCAGCAG CAGGCGGGCC GCGCAGCCAT GGGCGAGCTG 
TACGAGCGCG AGGTGCGCGA GATGCGCGGC GCAGTGCTGC GCTTGGGCGC GGCTCGCGGC 
CAGCTGCGCC TGGAGCAGGA GCACCTGCTG GAGGACATCG CGCACGTGCG CCAGCGCCTG 
GACGACGAGG CCCGGCAGCG CGAGGAGGCC GAGGCAGCGA CGCGCGCACT GGCCCGCTTT 
GCGCAGGAGG CCGAGGCGGC GCGCGTCGAG CTGCAGAAGA AGGCACAAGC CCTGCAGGAA 
GAGTGCGGCT ACCTGCGGCG TCACCACCAG GAGGAGGTGG GCGAGCTGCT CGGCCAGATC 
CAGAGCAGCA GCGCCGCGCA GACGCAGGCC GAGGCGCGCG ATGCCCTCAA GTGCGACGTG 
ACGTCGGCCC TGCGCGAGAT CCGCGCGCAG CTTGAAGGCC ACACGGTGCA GAGCACGCTT 
CAGTCCGAGG AGTGGTTCCG AGTGAGGCTG GACCGACTGT CCGAGGCAGC CAAGGTGAAC 
ACAGACGCCA TGCGCTCAGC ACAGGAGGAG ATATCTGAGT ACCGGCGCCA GCTGCAGGCC 
AGGACCACAG AGCTGGAGAC GCTCAAAAGC ACCAAGGACT CACTGGAGAG GCAGCGCTCT 
GAGCTGGAGG ACCGTCACCA GGCTGACATC GCATCCTACC AGGAGGCCAT CCAGCAGCTG 
GATGCAGAGC TGAGGAACAC CAAGTGGGAG ATGGCAGCCC AGCTCCGAGA GTACCAGGAT 
CTACTCAATG TCAAGATGGC TCTGGACATT GAGATCGCTG CTTACAGAAA ACTCCTGGAG 
GGTGAAGAAT GTCGAATTGG CTTTGGCCCG AGTCCTTTCC TCCTTCCAGA AGGACTCCCC 
AAGATCCCCT CCGTGTCCAC TCACATCAAA GTCAAAAGTG AAGAGAAGAT CAAGATGGTA 
GAAAAGTCAG AGAAGGAAAC TGTGATTGTG GAGGAACAGA CAGAGGAGGT CCAAGTGACT 
GAAGAAGTGA CTGAAGAAGA AGAGAAAGAG GCCAAAGAGG AGGAAGGAGA AGCAGTAAAG 
TCTCCCCCAG CAGAAGAGGC TGCATCCCCA GAGAAGGAGG AGGCCAAGTC ACCAGCCGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAGG AGGCCAAGTC CCCACAGAAG 
GAAGAGGCCA AGTCACCAGC TGAGGTGAAG TCCCCTGAGA AGGCCAAGTC CCCCGTGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAAG AAGCCAAATC CCCAGAGAAG 
GCCAAGTCCC CAGTGGAGGC CAAGTCTCCA GAAAAGGCCA AGTCCCCCAT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGT CAAATCCCCA GTGGAGGCCA AATCCCCAGA GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GAGAAAGCCA AGTCCCCTGT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GTGGAAGCCA AGTCCCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT GTGAAGGAGG AGGCCAAGTC CCCAGAGAAG 
GCCAAGTCTC CTGTGAAGGA GGAGGCCAAG TCCCCAGAGA AGGCCAAGTC TCCTGTGAAA 
GAGGAGGCCA AGTCCCCAGA GAAGGCCAAG TCTCCTGTGA AGGAGGAGGC CAAGTCGCCA 
GAGAAGGCCA AGTCTCCTGT GAAAGAGGAG GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT 
GTGAAAGAGG AGGCCAAGTC CCCAGAGAAG GCCAAGTCCC CAGTAGAGGC CAAGTCCCCA 
GAGAAGGCCA AGTCTCCTGC GAAGGAGGAG GCCAAGTCGC CAGAGAAGGC CAAGTCTCCT 
GTGAAAGAGG AGGCCAAGTC CCCAGAGAAG GCCAAGTCTC CTGTGAAAGA GGAGGCCAAG 
TCCCCAGAGA AGGCCAAGTC TCCTGCGAAG GAGGAGGCCA AGTCCCCAGA GAAGGCCAAG 
TCCCCAGAGA AGCCCAAAAG CCCTGTGAAG GAGGAGGCCA AGTCCCCAGA GAAGCCCAAA 
AGCCCTGTGA AGGAGGAGGC CAAGTCCCCA GAGAAGCCCA AAAGCCCTGT CAAGGAGGAG 
GCCAAATCCC CAGAGAAGGC CAAGTCTCCT GTGAAGGATG AGGCCAAGGC TCCTGGGAAG 
GAGGTCCCAA AGAAGGAGGA GGCCAAGTCC CCTGTGAAGG AGGAAGAAAA ACCCCAGGAG 
GTGAGAGCCA AAGAGCCCCC AAAGAAGGCA GAGGAAGAGA AAGCTCCAGC CATACCCAAA 
GCTGAGGAGA AGAAGGACAG CAAGAAAGAT GAGGTGCCCA AGAAGGAGGC TCCAAAGCCC 
GAGGAGAAGA AGGAGCCTGC TGTGGAAAAG CCCAAAGAAC CCAAAGCTGA AGCCAAGAAG 
GAAGAGGCTG AAGATAAGAA AAAAGCAACC ACCCCAGAGA AGGAGGCTCC TGCCAAGGGG 
AAGGAAGAGG CCAAGCCTAA AGAGAAGACT GAGGTGGCCA AGAAGGAACC AGATGATGCC 
AAGCCCAAAG AACCCAGCAA AGCAGCAGAA AAGCCAAAGA AGGAAGAGAC GCCGGCAGCA 
CCGGAGAAAA AAGACGCCAA GGAGGGGAAG GCCGCAGAAG CCAAGAAGCC TGAGGAGAAA 
CCCAAAACAG AGGCCCAAGC CAAGGAACCT GGCAAGGAGG CCTCCACACC CGGGAAAGCC 
AAGGCTGAGA AATCCTCCAG CACAGACCAG AAAGACAGCA GACCTGCAGA GAAGGCCGCA 
GAAGATAAGG CCACCAAGGG AGAGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEFH ( XM_024977820.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024833588.1 Bos taurus neurofilament heavy (NEFH), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa224692
Clone ID Related Accession (Same CDS sequence)	XM_024977820.1
Accession Version	XM_024977820.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	neurofilament heavy polypeptide isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCA GCGGCGCCGA CGCGCTGCTG GGCGCCTTCG CGCCGCTCCA CGGAGGCGGC 
AGCTTGCACT ATGCACTGGC TCGCAAAGGC GGCGCCGGCG GAGCGCGCTC TGCAGCCGGC 
TCCTCCAGCG GATTCCACTC ATGGGCGCGG ACCTCCGTGA GCTCCGTGTC GGCCTCCCCG 
AGCCGCTTCC GAGGCGCCGG GACTACCTCC AGCACCGACT CGCTAGACAC CCTGAGCAAC 
GGACCGGAGG GCTGCGTGGT GGTGGCGCGC AGTGAGAAGG AGCAGCTGCA GGTGCTGAAC 
GACCGCTTCG CGGGCTACAT CGACAAGGTG CGGCAGCTCG AGGCGCATAA CCGCAGCCTG 
GAGGGCGAGG CGGCGGCGCT GCGGCAGCAG CAGGCGGGCC GCGCAGCCAT GGGCGAGCTG 
TACGAGCGCG AGGTGCGCGA GATGCGCGGC GCAGTGCTGC GCTTGGGCGC GGCTCGCGGC 
CAGCTGCGCC TGGAGCAGGA GCACCTGCTG GAGGACATCG CGCACGTGCG CCAGCGCCTG 
GACGACGAGG CCCGGCAGCG CGAGGAGGCC GAGGCAGCGA CGCGCGCACT GGCCCGCTTT 
GCGCAGGAGG CCGAGGCGGC GCGCGTCGAG CTGCAGAAGA AGGCACAAGC CCTGCAGGAA 
GAGTGCGGCT ACCTGCGGCG TCACCACCAG GAGGAGGTGG GCGAGCTGCT CGGCCAGATC 
CAGAGCAGCA GCGCCGCGCA GACGCAGGCC GAGGCGCGCG ATGCCCTCAA GTGCGACGTG 
ACGTCGGCCC TGCGCGAGAT CCGCGCGCAG CTTGAAGGCC ACACGGTGCA GAGCACGCTT 
CAGTCCGAGG AGTGGTTCCG AGTGAGGCTG GACCGACTGT CCGAGGCAGC CAAGGTGAAC 
ACAGACGCCA TGCGCTCAGC ACAGGAGGAG ATATCTGAGT ACCGGCGCCA GCTGCAGGCC 
AGGACCACAG AGCTGGAGAC GCTCAAAAGC ACCAAGGACT CACTGGAGAG GCAGCGCTCT 
GAGCTGGAGG ACCGTCACCA GGCTGACATC GCATCCTACC AGGAGGCCAT CCAGCAGCTG 
GATGCAGAGC TGAGGAACAC CAAGTGGGAG ATGGCAGCCC AGCTCCGAGA GTACCAGGAT 
CTACTCAATG TCAAGATGGC TCTGGACATT GAGATCGCTG CTTACAGAAA ACTCCTGGAG 
GGTGAAGAAT GTCGAATTGG CTTTGGCCCG AGTCCTTTCC TCCTTCCAGA AGGACTCCCC 
AAGATCCCCT CCGTGTCCAC TCACATCAAA GTCAAAAGTG AAGAGAAGAT CAAGATGGTA 
GAAAAGTCAG AGAAGGAAAC TGTGATTGTG GAGGAACAGA CAGAGGAGGT CCAAGTGACT 
GAAGAAGTGA CTGAAGAAGA AGAGAAAGAG GCCAAAGAGG AGGAAGGAGA AGCAGTAAAG 
TCTCCCCCAG CAGAAGAGGC TGCATCCCCA GAGAAGGAGG AGGCCAAGTC ACCAGCCGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAGG AGGCCAAGTC CCCACAGAAG 
GAAGAGGCCA AGTCACCAGC TGAGGTGAAG TCCCCTGAGA AGGCCAAGTC CCCCGTGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAAG AAGCCAAATC CCCAGAGAAG 
GCCAAGTCCC CAGTGGAGGC CAAGTCTCCA GAAAAGGCCA AGTCCCCCAT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGT CAAATCCCCA GTGGAGGCCA AATCCCCAGA GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GAGAAAGCCA AGTCCCCTGT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GTGGAAGCCA AGTCCCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCA GTGGAGGCCA AGTCGCCAGA GAAGGCCAAG 
TCTCCTGTGA AGGAGGAGGC CAAGTCCCCA GAGAAGGCCA AGTCTCCTGT GAAAGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT GTGAAGGAGG AGGCCAAGTC GCCAGAGAAG 
GCCAAGTCTC CTGTGAAAGA GGAGGCCAAG TCCCCAGAGA AGGCCAAGTC TCCTGTGAAA 
GAGGAGGCCA AGTCCCCAGA GAAGGCCAAG TCCCCAGTAG AGGCCAAGTC CCCAGAGAAG 
GCCAAGTCTC CTGCGAAGGA GGAGGCCAAG TCGCCAGAGA AGGCCAAGTC TCCTGTGAAA 
GAGGAGGCCA AGTCCCCAGA GAAGGCCAAG TCTCCTGTGA AAGAGGAGGC CAAGTCCCCA 
GAGAAGGCCA AGTCTCCTGC GAAGGAGGAG GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA 
GAGAAGCCCA AAAGCCCTGT GAAGGAGGAG GCCAAGTCCC CAGAGAAGCC CAAAAGCCCT 
GTGAAGGAGG AGGCCAAGTC CCCAGAGAAG CCCAAAAGCC CTGTCAAGGA GGAGGCCAAA 
TCCCCAGAGA AGGCCAAGTC TCCTGTGAAG GATGAGGCCA AGGCTCCTGG GAAGGAGGTC 
CCAAAGAAGG AGGAGGCCAA GTCCCCTGTG AAGGAGGAAG AAAAACCCCA GGAGGTGAGA 
GCCAAAGAGC CCCCAAAGAA GGCAGAGGAA GAGAAAGCTC CAGCCATACC CAAAGCTGAG 
GAGAAGAAGG ACAGCAAGAA AGATGAGGTG CCCAAGAAGG AGGCTCCAAA GCCCGAGGAG 
AAGAAGGAGC CTGCTGTGGA AAAGCCCAAA GAACCCAAAG CTGAAGCCAA GAAGGAAGAG 
GCTGAAGATA AGAAAAAAGC AACCACCCCA GAGAAGGAGG CTCCTGCCAA GGGGAAGGAA 
GAGGCCAAGC CTAAAGAGAA GACTGAGGTG GCCAAGAAGG AACCAGATGA TGCCAAGCCC 
AAAGAACCCA GCAAAGCAGC AGAAAAGCCA AAGAAGGAAG AGACGCCGGC AGCACCGGAG 
AAAAAAGACG CCAAGGAGGG GAAGGCCGCA GAAGCCAAGA AGCCTGAGGA GAAACCCAAA 
ACAGAGGCCC AAGCCAAGGA ACCTGGCAAG GAGGCCTCCA CACCCGGGAA AGCCAAGGCT 
GAGAAATCCT CCAGCACAGA CCAGAAAGAC AGCAGACCTG CAGAGAAGGC CGCAGAAGAT 
AAGGCCACCA AGGGAGAGAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024833588.1
CDS	76..3279
Translation	MSFSGADALLGAFAPLHGGGSLHYALARKGGAGGARSAAGSSSGFHSWARTSVSSVSASPSRFRGAGTTSSTDSLDTLSNGPEGCVVVARSEKEQLQVLNDRFAGYIDKVRQLEAHNRSLEGEAAALRQQQAGRAAMGELYEREVREMRGAVLRLGAARGQLRLEQEHLLEDIAHVRQRLDDEARQREEAEAATRALARFAQEAEAARVELQKKAQALQEECGYLRRHHQEEVGELLGQIQSSSAAQTQAEARDALKCDVTSALREIRAQLEGHTVQSTLQSEEWFRVRLDRLSEAAKVNTDAMRSAQEEISEYRRQLQARTTELETLKSTKDSLERQRSELEDRHQADIASYQEAIQQLDAELRNTKWEMAAQLREYQDLLNVKMALDIEIAAYRKLLEGEECRIGFGPSPFLLPEGLPKIPSVSTHIKVKSEEKIKMVEKSEKETVIVEEQTEEVQVTEEVTEEEEKEAKEEEGEAVKSPPAEEAASPEKEEAKSPAEAKSPEKAKSPVKEEAKSPQKEEAKSPAEVKSPEKAKSPVEAKSPEKAKSPVKEEAKSPEKAKSPVEAKSPEKAKSPMKEEAKSPEKVKSPVEAKSPEKEEAKSPEKAKSPEKAKSPVKEEAKSPEKAKSPVEAKSPVKEEAKSPEKAKSPVEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVEAKSPEKAKSPAKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPAKEEAKSPEKAKSPEKPKSPVKEEAKSPEKPKSPVKEEAKSPEKPKSPVKEEAKSPEKAKSPVKDEAKAPGKEVPKKEEAKSPVKEEEKPQEVRAKEPPKKAEEEKAPAIPKAEEKKDSKKDEVPKKEAPKPEEKKEPAVEKPKEPKAEAKKEEAEDKKKATTPEKEAPAKGKEEAKPKEKTEVAKKEPDDAKPKEPSKAAEKPKKEETPAAPEKKDAKEGKAAEAKKPEEKPKTEAQAKEPGKEASTPGKAKAEKSSSTDQKDSRPAEKAAEDKATKGEK

Target ORF information:

RefSeq Version	XM_024977820.1
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofilament heavy (NEFH), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024977820.1

ORF Insert Sequence:

ATGAGCTTCA GCGGCGCCGA CGCGCTGCTG GGCGCCTTCG CGCCGCTCCA CGGAGGCGGC 
AGCTTGCACT ATGCACTGGC TCGCAAAGGC GGCGCCGGCG GAGCGCGCTC TGCAGCCGGC 
TCCTCCAGCG GATTCCACTC ATGGGCGCGG ACCTCCGTGA GCTCCGTGTC GGCCTCCCCG 
AGCCGCTTCC GAGGCGCCGG GACTACCTCC AGCACCGACT CGCTAGACAC CCTGAGCAAC 
GGACCGGAGG GCTGCGTGGT GGTGGCGCGC AGTGAGAAGG AGCAGCTGCA GGTGCTGAAC 
GACCGCTTCG CGGGCTACAT CGACAAGGTG CGGCAGCTCG AGGCGCATAA CCGCAGCCTG 
GAGGGCGAGG CGGCGGCGCT GCGGCAGCAG CAGGCGGGCC GCGCAGCCAT GGGCGAGCTG 
TACGAGCGCG AGGTGCGCGA GATGCGCGGC GCAGTGCTGC GCTTGGGCGC GGCTCGCGGC 
CAGCTGCGCC TGGAGCAGGA GCACCTGCTG GAGGACATCG CGCACGTGCG CCAGCGCCTG 
GACGACGAGG CCCGGCAGCG CGAGGAGGCC GAGGCAGCGA CGCGCGCACT GGCCCGCTTT 
GCGCAGGAGG CCGAGGCGGC GCGCGTCGAG CTGCAGAAGA AGGCACAAGC CCTGCAGGAA 
GAGTGCGGCT ACCTGCGGCG TCACCACCAG GAGGAGGTGG GCGAGCTGCT CGGCCAGATC 
CAGAGCAGCA GCGCCGCGCA GACGCAGGCC GAGGCGCGCG ATGCCCTCAA GTGCGACGTG 
ACGTCGGCCC TGCGCGAGAT CCGCGCGCAG CTTGAAGGCC ACACGGTGCA GAGCACGCTT 
CAGTCCGAGG AGTGGTTCCG AGTGAGGCTG GACCGACTGT CCGAGGCAGC CAAGGTGAAC 
ACAGACGCCA TGCGCTCAGC ACAGGAGGAG ATATCTGAGT ACCGGCGCCA GCTGCAGGCC 
AGGACCACAG AGCTGGAGAC GCTCAAAAGC ACCAAGGACT CACTGGAGAG GCAGCGCTCT 
GAGCTGGAGG ACCGTCACCA GGCTGACATC GCATCCTACC AGGAGGCCAT CCAGCAGCTG 
GATGCAGAGC TGAGGAACAC CAAGTGGGAG ATGGCAGCCC AGCTCCGAGA GTACCAGGAT 
CTACTCAATG TCAAGATGGC TCTGGACATT GAGATCGCTG CTTACAGAAA ACTCCTGGAG 
GGTGAAGAAT GTCGAATTGG CTTTGGCCCG AGTCCTTTCC TCCTTCCAGA AGGACTCCCC 
AAGATCCCCT CCGTGTCCAC TCACATCAAA GTCAAAAGTG AAGAGAAGAT CAAGATGGTA 
GAAAAGTCAG AGAAGGAAAC TGTGATTGTG GAGGAACAGA CAGAGGAGGT CCAAGTGACT 
GAAGAAGTGA CTGAAGAAGA AGAGAAAGAG GCCAAAGAGG AGGAAGGAGA AGCAGTAAAG 
TCTCCCCCAG CAGAAGAGGC TGCATCCCCA GAGAAGGAGG AGGCCAAGTC ACCAGCCGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAGG AGGCCAAGTC CCCACAGAAG 
GAAGAGGCCA AGTCACCAGC TGAGGTGAAG TCCCCTGAGA AGGCCAAGTC CCCCGTGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAAG AAGCCAAATC CCCAGAGAAG 
GCCAAGTCCC CAGTGGAGGC CAAGTCTCCA GAAAAGGCCA AGTCCCCCAT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGT CAAATCCCCA GTGGAGGCCA AATCCCCAGA GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GAGAAAGCCA AGTCCCCTGT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GTGGAAGCCA AGTCCCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCA GTGGAGGCCA AGTCGCCAGA GAAGGCCAAG 
TCTCCTGTGA AGGAGGAGGC CAAGTCCCCA GAGAAGGCCA AGTCTCCTGT GAAAGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT GTGAAGGAGG AGGCCAAGTC GCCAGAGAAG 
GCCAAGTCTC CTGTGAAAGA GGAGGCCAAG TCCCCAGAGA AGGCCAAGTC TCCTGTGAAA 
GAGGAGGCCA AGTCCCCAGA GAAGGCCAAG TCCCCAGTAG AGGCCAAGTC CCCAGAGAAG 
GCCAAGTCTC CTGCGAAGGA GGAGGCCAAG TCGCCAGAGA AGGCCAAGTC TCCTGTGAAA 
GAGGAGGCCA AGTCCCCAGA GAAGGCCAAG TCTCCTGTGA AAGAGGAGGC CAAGTCCCCA 
GAGAAGGCCA AGTCTCCTGC GAAGGAGGAG GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA 
GAGAAGCCCA AAAGCCCTGT GAAGGAGGAG GCCAAGTCCC CAGAGAAGCC CAAAAGCCCT 
GTGAAGGAGG AGGCCAAGTC CCCAGAGAAG CCCAAAAGCC CTGTCAAGGA GGAGGCCAAA 
TCCCCAGAGA AGGCCAAGTC TCCTGTGAAG GATGAGGCCA AGGCTCCTGG GAAGGAGGTC 
CCAAAGAAGG AGGAGGCCAA GTCCCCTGTG AAGGAGGAAG AAAAACCCCA GGAGGTGAGA 
GCCAAAGAGC CCCCAAAGAA GGCAGAGGAA GAGAAAGCTC CAGCCATACC CAAAGCTGAG 
GAGAAGAAGG ACAGCAAGAA AGATGAGGTG CCCAAGAAGG AGGCTCCAAA GCCCGAGGAG 
AAGAAGGAGC CTGCTGTGGA AAAGCCCAAA GAACCCAAAG CTGAAGCCAA GAAGGAAGAG 
GCTGAAGATA AGAAAAAAGC AACCACCCCA GAGAAGGAGG CTCCTGCCAA GGGGAAGGAA 
GAGGCCAAGC CTAAAGAGAA GACTGAGGTG GCCAAGAAGG AACCAGATGA TGCCAAGCCC 
AAAGAACCCA GCAAAGCAGC AGAAAAGCCA AAGAAGGAAG AGACGCCGGC AGCACCGGAG 
AAAAAAGACG CCAAGGAGGG GAAGGCCGCA GAAGCCAAGA AGCCTGAGGA GAAACCCAAA 
ACAGAGGCCC AAGCCAAGGA ACCTGGCAAG GAGGCCTCCA CACCCGGGAA AGCCAAGGCT 
GAGAAATCCT CCAGCACAGA CCAGAAAGAC AGCAGACCTG CAGAGAAGGC CGCAGAAGAT 
AAGGCCACCA AGGGAGAGAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEFH ( XM_024977821.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024833589.1 Bos taurus neurofilament heavy (NEFH), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBa224693
Clone ID Related Accession (Same CDS sequence)	XM_024977821.1
Accession Version	XM_024977821.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3084bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	neurofilament heavy polypeptide isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCA GCGGCGCCGA CGCGCTGCTG GGCGCCTTCG CGCCGCTCCA CGGAGGCGGC 
AGCTTGCACT ATGCACTGGC TCGCAAAGGC GGCGCCGGCG GAGCGCGCTC TGCAGCCGGC 
TCCTCCAGCG GATTCCACTC ATGGGCGCGG ACCTCCGTGA GCTCCGTGTC GGCCTCCCCG 
AGCCGCTTCC GAGGCGCCGG GACTACCTCC AGCACCGACT CGCTAGACAC CCTGAGCAAC 
GGACCGGAGG GCTGCGTGGT GGTGGCGCGC AGTGAGAAGG AGCAGCTGCA GGTGCTGAAC 
GACCGCTTCG CGGGCTACAT CGACAAGGTG CGGCAGCTCG AGGCGCATAA CCGCAGCCTG 
GAGGGCGAGG CGGCGGCGCT GCGGCAGCAG CAGGCGGGCC GCGCAGCCAT GGGCGAGCTG 
TACGAGCGCG AGGTGCGCGA GATGCGCGGC GCAGTGCTGC GCTTGGGCGC GGCTCGCGGC 
CAGCTGCGCC TGGAGCAGGA GCACCTGCTG GAGGACATCG CGCACGTGCG CCAGCGCCTG 
GACGACGAGG CCCGGCAGCG CGAGGAGGCC GAGGCAGCGA CGCGCGCACT GGCCCGCTTT 
GCGCAGGAGG CCGAGGCGGC GCGCGTCGAG CTGCAGAAGA AGGCACAAGC CCTGCAGGAA 
GAGTGCGGCT ACCTGCGGCG TCACCACCAG GAGGAGGTGG GCGAGCTGCT CGGCCAGATC 
CAGAGCAGCA GCGCCGCGCA GACGCAGGCC GAGGCGCGCG ATGCCCTCAA GTGCGACGTG 
ACGTCGGCCC TGCGCGAGAT CCGCGCGCAG CTTGAAGGCC ACACGGTGCA GAGCACGCTT 
CAGTCCGAGG AGTGGTTCCG AGTGAGGCTG GACCGACTGT CCGAGGCAGC CAAGGTGAAC 
ACAGACGCCA TGCGCTCAGC ACAGGAGGAG ATATCTGAGT ACCGGCGCCA GCTGCAGGCC 
AGGACCACAG AGCTGGAGAC GCTCAAAAGC ACCAAGGACT CACTGGAGAG GCAGCGCTCT 
GAGCTGGAGG ACCGTCACCA GGCTGACATC GCATCCTACC AGGAGGCCAT CCAGCAGCTG 
GATGCAGAGC TGAGGAACAC CAAGTGGGAG ATGGCAGCCC AGCTCCGAGA GTACCAGGAT 
CTACTCAATG TCAAGATGGC TCTGGACATT GAGATCGCTG CTTACAGAAA ACTCCTGGAG 
GGTGAAGAAT GTCGAATTGG CTTTGGCCCG AGTCCTTTCC TCCTTCCAGA AGGACTCCCC 
AAGATCCCCT CCGTGTCCAC TCACATCAAA GTCAAAAGTG AAGAGAAGAT CAAGATGGTA 
GAAAAGTCAG AGAAGGAAAC TGTGATTGTG GAGGAACAGA CAGAGGAGGT CCAAGTGACT 
GAAGAAGTGA CTGAAGAAGA AGAGAAAGAG GCCAAAGAGG AGGAAGGAGA AGCAGTAAAG 
TCTCCCCCAG CAGAAGAGGC TGCATCCCCA GAGAAGGAGG AGGCCAAGTC ACCAGCCGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAGG AGGCCAAGTC CCCACAGAAG 
GAAGAGGCCA AGTCACCAGC TGAGGTGAAG TCCCCTGAGA AGGCCAAGTC CCCCGTGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAAG AAGCCAAATC CCCAGAGAAG 
GCCAAGTCCC CAGTGGAGGC CAAGTCTCCA GAAAAGGCCA AGTCCCCCAT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGT CAAATCCCCA GTGGAGGCCA AATCCCCAGA GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GAGAAAGCCA AGTCCCCTGT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GTGGAAGCCA AGTCCCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCA GTGGAGGCCA AGTCGCCAGA GAAGGCCAAG 
TCTCCTGTGA AGGAGGAGGC CAAGTCCCCA GAGAAGGCCA AGTCTCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT GTGAAAGAGG AGGCCAAGTC CCCAGAGAAG 
GCCAAGTCTC CTGTGAAGGA GGAGGCCAAG TCGCCAGAGA AGGCCAAGTC TCCTGTGAAA 
GAGGAGGCCA AGTCCCCAGA GAAGGCCAAG TCTCCTGTGA AAGAGGAGGC CAAGTCCCCA 
GAGAAGGCCA AGTCTCCTGC GAAGGAGGAG GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA 
GAGAAGCCCA AAAGCCCTGT GAAGGAGGAG GCCAAGTCCC CAGAGAAGCC CAAAAGCCCT 
GTGAAGGAGG AGGCCAAGTC CCCAGAGAAG CCCAAAAGCC CTGTCAAGGA GGAGGCCAAA 
TCCCCAGAGA AGGCCAAGTC TCCTGTGAAG GATGAGGCCA AGGCTCCTGG GAAGGAGGTC 
CCAAAGAAGG AGGAGGCCAA GTCCCCTGTG AAGGAGGAAG AAAAACCCCA GGAGGTGAGA 
GCCAAAGAGC CCCCAAAGAA GGCAGAGGAA GAGAAAGCTC CAGCCATACC CAAAGCTGAG 
GAGAAGAAGG ACAGCAAGAA AGATGAGGTG CCCAAGAAGG AGGCTCCAAA GCCCGAGGAG 
AAGAAGGAGC CTGCTGTGGA AAAGCCCAAA GAACCCAAAG CTGAAGCCAA GAAGGAAGAG 
GCTGAAGATA AGAAAAAAGC AACCACCCCA GAGAAGGAGG CTCCTGCCAA GGGGAAGGAA 
GAGGCCAAGC CTAAAGAGAA GACTGAGGTG GCCAAGAAGG AACCAGATGA TGCCAAGCCC 
AAAGAACCCA GCAAAGCAGC AGAAAAGCCA AAGAAGGAAG AGACGCCGGC AGCACCGGAG 
AAAAAAGACG CCAAGGAGGG GAAGGCCGCA GAAGCCAAGA AGCCTGAGGA GAAACCCAAA 
ACAGAGGCCC AAGCCAAGGA ACCTGGCAAG GAGGCCTCCA CACCCGGGAA AGCCAAGGCT 
GAGAAATCCT CCAGCACAGA CCAGAAAGAC AGCAGACCTG CAGAGAAGGC CGCAGAAGAT 
AAGGCCACCA AGGGAGAGAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024833589.1
CDS	76..3159
Translation	MSFSGADALLGAFAPLHGGGSLHYALARKGGAGGARSAAGSSSGFHSWARTSVSSVSASPSRFRGAGTTSSTDSLDTLSNGPEGCVVVARSEKEQLQVLNDRFAGYIDKVRQLEAHNRSLEGEAAALRQQQAGRAAMGELYEREVREMRGAVLRLGAARGQLRLEQEHLLEDIAHVRQRLDDEARQREEAEAATRALARFAQEAEAARVELQKKAQALQEECGYLRRHHQEEVGELLGQIQSSSAAQTQAEARDALKCDVTSALREIRAQLEGHTVQSTLQSEEWFRVRLDRLSEAAKVNTDAMRSAQEEISEYRRQLQARTTELETLKSTKDSLERQRSELEDRHQADIASYQEAIQQLDAELRNTKWEMAAQLREYQDLLNVKMALDIEIAAYRKLLEGEECRIGFGPSPFLLPEGLPKIPSVSTHIKVKSEEKIKMVEKSEKETVIVEEQTEEVQVTEEVTEEEEKEAKEEEGEAVKSPPAEEAASPEKEEAKSPAEAKSPEKAKSPVKEEAKSPQKEEAKSPAEVKSPEKAKSPVEAKSPEKAKSPVKEEAKSPEKAKSPVEAKSPEKAKSPMKEEAKSPEKVKSPVEAKSPEKEEAKSPEKAKSPEKAKSPVKEEAKSPEKAKSPVEAKSPVKEEAKSPEKAKSPVEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPAKEEAKSPEKAKSPEKPKSPVKEEAKSPEKPKSPVKEEAKSPEKPKSPVKEEAKSPEKAKSPVKDEAKAPGKEVPKKEEAKSPVKEEEKPQEVRAKEPPKKAEEEKAPAIPKAEEKKDSKKDEVPKKEAPKPEEKKEPAVEKPKEPKAEAKKEEAEDKKKATTPEKEAPAKGKEEAKPKEKTEVAKKEPDDAKPKEPSKAAEKPKKEETPAAPEKKDAKEGKAAEAKKPEEKPKTEAQAKEPGKEASTPGKAKAEKSSSTDQKDSRPAEKAAEDKATKGEK

Target ORF information:

RefSeq Version	XM_024977821.1
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofilament heavy (NEFH), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024977821.1

ORF Insert Sequence:

ATGAGCTTCA GCGGCGCCGA CGCGCTGCTG GGCGCCTTCG CGCCGCTCCA CGGAGGCGGC 
AGCTTGCACT ATGCACTGGC TCGCAAAGGC GGCGCCGGCG GAGCGCGCTC TGCAGCCGGC 
TCCTCCAGCG GATTCCACTC ATGGGCGCGG ACCTCCGTGA GCTCCGTGTC GGCCTCCCCG 
AGCCGCTTCC GAGGCGCCGG GACTACCTCC AGCACCGACT CGCTAGACAC CCTGAGCAAC 
GGACCGGAGG GCTGCGTGGT GGTGGCGCGC AGTGAGAAGG AGCAGCTGCA GGTGCTGAAC 
GACCGCTTCG CGGGCTACAT CGACAAGGTG CGGCAGCTCG AGGCGCATAA CCGCAGCCTG 
GAGGGCGAGG CGGCGGCGCT GCGGCAGCAG CAGGCGGGCC GCGCAGCCAT GGGCGAGCTG 
TACGAGCGCG AGGTGCGCGA GATGCGCGGC GCAGTGCTGC GCTTGGGCGC GGCTCGCGGC 
CAGCTGCGCC TGGAGCAGGA GCACCTGCTG GAGGACATCG CGCACGTGCG CCAGCGCCTG 
GACGACGAGG CCCGGCAGCG CGAGGAGGCC GAGGCAGCGA CGCGCGCACT GGCCCGCTTT 
GCGCAGGAGG CCGAGGCGGC GCGCGTCGAG CTGCAGAAGA AGGCACAAGC CCTGCAGGAA 
GAGTGCGGCT ACCTGCGGCG TCACCACCAG GAGGAGGTGG GCGAGCTGCT CGGCCAGATC 
CAGAGCAGCA GCGCCGCGCA GACGCAGGCC GAGGCGCGCG ATGCCCTCAA GTGCGACGTG 
ACGTCGGCCC TGCGCGAGAT CCGCGCGCAG CTTGAAGGCC ACACGGTGCA GAGCACGCTT 
CAGTCCGAGG AGTGGTTCCG AGTGAGGCTG GACCGACTGT CCGAGGCAGC CAAGGTGAAC 
ACAGACGCCA TGCGCTCAGC ACAGGAGGAG ATATCTGAGT ACCGGCGCCA GCTGCAGGCC 
AGGACCACAG AGCTGGAGAC GCTCAAAAGC ACCAAGGACT CACTGGAGAG GCAGCGCTCT 
GAGCTGGAGG ACCGTCACCA GGCTGACATC GCATCCTACC AGGAGGCCAT CCAGCAGCTG 
GATGCAGAGC TGAGGAACAC CAAGTGGGAG ATGGCAGCCC AGCTCCGAGA GTACCAGGAT 
CTACTCAATG TCAAGATGGC TCTGGACATT GAGATCGCTG CTTACAGAAA ACTCCTGGAG 
GGTGAAGAAT GTCGAATTGG CTTTGGCCCG AGTCCTTTCC TCCTTCCAGA AGGACTCCCC 
AAGATCCCCT CCGTGTCCAC TCACATCAAA GTCAAAAGTG AAGAGAAGAT CAAGATGGTA 
GAAAAGTCAG AGAAGGAAAC TGTGATTGTG GAGGAACAGA CAGAGGAGGT CCAAGTGACT 
GAAGAAGTGA CTGAAGAAGA AGAGAAAGAG GCCAAAGAGG AGGAAGGAGA AGCAGTAAAG 
TCTCCCCCAG CAGAAGAGGC TGCATCCCCA GAGAAGGAGG AGGCCAAGTC ACCAGCCGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAGG AGGCCAAGTC CCCACAGAAG 
GAAGAGGCCA AGTCACCAGC TGAGGTGAAG TCCCCTGAGA AGGCCAAGTC CCCCGTGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAAG AAGCCAAATC CCCAGAGAAG 
GCCAAGTCCC CAGTGGAGGC CAAGTCTCCA GAAAAGGCCA AGTCCCCCAT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGT CAAATCCCCA GTGGAGGCCA AATCCCCAGA GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GAGAAAGCCA AGTCCCCTGT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GTGGAAGCCA AGTCCCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCA GTGGAGGCCA AGTCGCCAGA GAAGGCCAAG 
TCTCCTGTGA AGGAGGAGGC CAAGTCCCCA GAGAAGGCCA AGTCTCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT GTGAAAGAGG AGGCCAAGTC CCCAGAGAAG 
GCCAAGTCTC CTGTGAAGGA GGAGGCCAAG TCGCCAGAGA AGGCCAAGTC TCCTGTGAAA 
GAGGAGGCCA AGTCCCCAGA GAAGGCCAAG TCTCCTGTGA AAGAGGAGGC CAAGTCCCCA 
GAGAAGGCCA AGTCTCCTGC GAAGGAGGAG GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA 
GAGAAGCCCA AAAGCCCTGT GAAGGAGGAG GCCAAGTCCC CAGAGAAGCC CAAAAGCCCT 
GTGAAGGAGG AGGCCAAGTC CCCAGAGAAG CCCAAAAGCC CTGTCAAGGA GGAGGCCAAA 
TCCCCAGAGA AGGCCAAGTC TCCTGTGAAG GATGAGGCCA AGGCTCCTGG GAAGGAGGTC 
CCAAAGAAGG AGGAGGCCAA GTCCCCTGTG AAGGAGGAAG AAAAACCCCA GGAGGTGAGA 
GCCAAAGAGC CCCCAAAGAA GGCAGAGGAA GAGAAAGCTC CAGCCATACC CAAAGCTGAG 
GAGAAGAAGG ACAGCAAGAA AGATGAGGTG CCCAAGAAGG AGGCTCCAAA GCCCGAGGAG 
AAGAAGGAGC CTGCTGTGGA AAAGCCCAAA GAACCCAAAG CTGAAGCCAA GAAGGAAGAG 
GCTGAAGATA AGAAAAAAGC AACCACCCCA GAGAAGGAGG CTCCTGCCAA GGGGAAGGAA 
GAGGCCAAGC CTAAAGAGAA GACTGAGGTG GCCAAGAAGG AACCAGATGA TGCCAAGCCC 
AAAGAACCCA GCAAAGCAGC AGAAAAGCCA AAGAAGGAAG AGACGCCGGC AGCACCGGAG 
AAAAAAGACG CCAAGGAGGG GAAGGCCGCA GAAGCCAAGA AGCCTGAGGA GAAACCCAAA 
ACAGAGGCCC AAGCCAAGGA ACCTGGCAAG GAGGCCTCCA CACCCGGGAA AGCCAAGGCT 
GAGAAATCCT CCAGCACAGA CCAGAAAGAC AGCAGACCTG CAGAGAAGGC CGCAGAAGAT 
AAGGCCACCA AGGGAGAGAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEFH ( XM_005218112.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005218169.1 Bos taurus neurofilament, heavy polypeptide (NEFH), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa28068
Clone ID Related Accession (Same CDS sequence)	XM_005218112.2 , XM_005218112.3 , XM_005195129.3
Accession Version	XM_005218112.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neurofilament heavy polypeptide isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104458.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005218112.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCA GCGGCGCCGA CGCGCTGCTG GGCGCCTTCG CGCCGCTCCA CGGAGGCGGC 
AGCTTGCACT ATGCACTGGC TCGCAAAGGC GGCGCCGGCG GAGCGCGCTC TGCAGCCGGC 
TCCTCCAGCG GATTCCACTC ATGGGCGCGG ACCTCCGTGA GCTCCGTGTC GGCCTCCCCG 
AGCCGCTTCC GAGGCGCCGG GACTACCTCC AGCACCGACT CGCTAGACAC CCTGAGCAAC 
GGACCGGAGG GCTGCGTGGT GGTGGCGCGC AGTGAGAAGG AGCAGCTGCA GGTGCTGAAC 
GACCGCTTCG CGGGCTACAT CGACAAGGTG CGGCAGCTCG AGGCGCATAA CCGCAGCCTG 
GAGGGCGAGG CGGCGGCGCT GCGGCAGCAG CAGGCGGGCC GCGCAGCCAT GGGCGAGCTG 
TACGAGCGCG AGGTGCGCGA GATGCGCGGC GCAGTGCTGC GCTTGGGCGC GGCTCGCGGC 
CAGCTGCGCC TGGAGCAGGA GCACCTGCTG GAGGACATCG CGCACGTGCG CCAGCGCCTG 
GACGACGAGG CCCGGCAGCG CGAGGAGGCC GAGGCAGCGA CGCGCGCACT GGCCCGCTTT 
GCGCAGGAGG CCGAGGCGGC GCGCGTCGAG CTGCAGAAGA AGGCACAAGC CCTGCAGGAA 
GAGTGCGGCT ACCTGCGGCG TCACCACCAG GAGGAGGTGG GCGAGCTGCT CGGCCAGATC 
CAGAGCAGCA GCGCCGCGCA GACGCAGGCC GAGGCGCGCG ATGCCCTCAA GTGCGACGTG 
ACGTCGGCCC TGCGCGAGAT CCGCGCGCAG CTTGAAGGCC ACACGGTGCA GAGCACGCTT 
CAGTCCGAGG AGTGGTTCCG AGTGAGGCTG GACCGACTGT CCGAGGCAGC CAAGGTGAAC 
ACAGACGCCA TGCGCTCAGC ACAGGAGGAG ATATCTGAGT ACCGGCGCCA GCTGCAGGCC 
AGGACCACAG AGCTGGAGAC GCTCAAAAGC ACCAAGGACT CACTGGAGAG GCAGCGCTCT 
GAGCTGGAGG ACCGTCACCA GGCTGACATC GCATCCTACC AGGAGGCCAT CCAGCAGCTG 
GATGCAGAGC TGAGGAACAC CAAGTGGGAG ATGGCAGCCC AGCTCCGAGA GTACCAGGAT 
CTACTCAATG TCAAGATGGC TCTGGACATT GAGATCGCTG CTTACAGAAA ACTCCTGGAG 
GGTGAAGAAT GTCGAATTGG CTTTGGCCCG AGTCCTTTCC TCCTTCCAGA AGGACTCCCC 
AAGATCCCCT CCGTGTCCAC TCACATCAAA GTCAAAAGTG AAGAGAAGAT CAAGATGGTA 
GAAAAGTCAG AGAAGGAAAC TGTGATTGTG GAGGAACAGA CAGAGGAGGT CCAAGTGACT 
GAAGAAGTGA CTGAAGAAGA AGAGAAAGAG GCCAAAGAGG AGGAAGGAGA AGCAGTAAAG 
TCTCCCCCAG CAGAAGAGGC TGCATCCCCA GAGAAGGAGG AGGCCAAGTC ACCAGCCGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAGG AGGCCAAGTC CCCACAGAAG 
GAAGAGGCCA AGTCACCAGC TGAGGTGAAG TCCCCTGAGA AGGCCAAGTC CCCCGTGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAAG AAGCCAAATC CCCAGAGAAG 
GCCAAGTCCC CAGTGGAGGC CAAGTCTCCA GAAAAGGCCA AGTCCCCCAT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGT CAAATCCCCA GTGGAGGCCA AATCCCCAGA GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GAGAAAGCCA AGTCCCCTGT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GTGGAAGCCA AGTCCCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCA GTGGAGGCCA AGTCGCCAGA GAAGGCCAAG 
TCTCCTGTGA AGGAGGAGGC CAAGTCCCCA GAGAAGGCCA AGTCTCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT GTGAAAGAGG AGGCCAAGTC CCCAGAGAAG 
GCCAAGTCTC CTGTGAAGGA GGAGGCCAAG TCGCCAGAGA AGGCCAAGTC TCCTGTGAAA 
GAGGAGGCCA AGTCCCCAGA GAAGGCCAAG TCTCCTGTGA AAGAGGAGGC CAAGTCCCCA 
GAGAAGGCCA AGTCCCCAGT AGAGGCCAAG TCCCCAGAGA AGGCCAAGTC TCCTGCGAAG 
GAGGAGGCCA AGTCGCCAGA GAAGGCCAAG TCTCCTGTGA AAGAGGAGGC CAAGTCCCCA 
GAGAAGGCCA AGTCTCCTGT GAAAGAGGAG GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT 
GCGAAGGAGG AGGCCAAGTC CCCAGAGAAG GCCAAGTCCC CAGAGAAGCC CAAAAGCCCT 
GTGAAGGAGG AGGCCAAGTC CCCAGAGAAG CCCAAAAGCC CTGTGAAGGA GGAGGCCAAG 
TCCCCAGAGA AGCCCAAAAG CCCTGTCAAG GAGGAGGCCA AATCCCCAGA GAAGGCCAAG 
TCTCCTGTGA AGGATGAGGC CAAGGCTCCT GGGAAGGAGG TCCCAAAGAA GGAGGAGGCC 
AAGTCCCCTG TGAAGGAGGA AGAAAAACCC CAGGAGGTGA GAGCCAAAGA GCCCCCAAAG 
AAGGCAGAGG AAGAGAAAGC TCCAGCCATA CCCAAAGCTG AGGAGAAGAA GGACAGCAAG 
AAAGATGAGG TGCCCAAGAA GGAGGCTCCA AAGCCCGAGG AGAAGAAGGA GCCTGCTGTG 
GAAAAGCCCA AAGAACCCAA AGCTGAAGCC AAGAAGGAAG AGGCTGAAGA TAAGAAAAAA 
GCAACCACCC CAGAGAAGGA GGCTCCTGCC AAGGGGAAGG AAGAGGCCAA GCCTAAAGAG 
AAGACTGAGG TGGCCAAGAA GGAACCAGAT GATGCCAAGC CCAAAGAACC CAGCAAAGCA 
GCAGAAAAGC CAAAGAAGGA AGAGACGCCG GCAGCACCGG AGAAAAAAGA CGCCAAGGAG 
GGGAAGGCCG CAGAAGCCAA GAAGCCTGAG GAGAAACCCA AAACAGAGGC CCAAGCCAAG 
GAACCTGGCA AGGAGGCCTC CACACCCGGG AAAGCCAAGG CTGAGAAATC CTCCAGCACA 
GACCAGAAAG ACAGCAGACC TGCAGAGAAG GCCGCAGAAG ATAAGGCCAC CAAGGGAGAG 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005218169.1
CDS	79..3324
Translation	MSFSGADALLGAFAPLHGGGSLHYALARKGGAGGARSAAGSSSGFHSWARTSVSSVSASPSRFRGAGTTSSTDSLDTLSNGPEGCVVVARSEKEQLQVLNDRFAGYIDKVRQLEAHNRSLEGEAAALRQQQAGRAAMGELYEREVREMRGAVLRLGAARGQLRLEQEHLLEDIAHVRQRLDDEARQREEAEAATRALARFAQEAEAARVELQKKAQALQEECGYLRRHHQEEVGELLGQIQSSSAAQTQAEARDALKCDVTSALREIRAQLEGHTVQSTLQSEEWFRVRLDRLSEAAKVNTDAMRSAQEEISEYRRQLQARTTELETLKSTKDSLERQRSELEDRHQADIASYQEAIQQLDAELRNTKWEMAAQLREYQDLLNVKMALDIEIAAYRKLLEGEECRIGFGPSPFLLPEGLPKIPSVSTHIKVKSEEKIKMVEKSEKETVIVEEQTEEVQVTEEVTEEEEKEAKEEEGEAVKSPPAEEAASPEKEEAKSPAEAKSPEKAKSPVKEEAKSPQKEEAKSPAEVKSPEKAKSPVEAKSPEKAKSPVKEEAKSPEKAKSPVEAKSPEKAKSPMKEEAKSPEKVKSPVEAKSPEKEEAKSPEKAKSPEKAKSPVKEEAKSPEKAKSPVEAKSPVKEEAKSPEKAKSPVEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVEAKSPEKAKSPAKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPAKEEAKSPEKAKSPEKPKSPVKEEAKSPEKPKSPVKEEAKSPEKPKSPVKEEAKSPEKAKSPVKDEAKAPGKEVPKKEEAKSPVKEEEKPQEVRAKEPPKKAEEEKAPAIPKAEEKKDSKKDEVPKKEAPKPEEKKEPAVEKPKEPKAEAKKEEAEDKKKATTPEKEAPAKGKEEAKPKEKTEVAKKEPDDAKPKEPSKAAEKPKKEETPAAPEKKDAKEGKAAEAKKPEEKPKTEAQAKEPGKEASTPGKAKAEKSSSTDQKDSRPAEKAAEDKATKGEK

Target ORF information:

RefSeq Version	XM_005218112.2
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofilament, heavy polypeptide (NEFH), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005218112.2

ORF Insert Sequence:

ATGAGCTTCA GCGGCGCCGA CGCGCTGCTG GGCGCCTTCG CGCCGCTCCA CGGAGGCGGC 
AGCTTGCACT ATGCACTGGC TCGCAAAGGC GGCGCCGGCG GAGCGCGCTC TGCAGCCGGC 
TCCTCCAGCG GATTCCACTC ATGGGCGCGG ACCTCCGTGA GCTCCGTGTC GGCCTCCCCG 
AGCCGCTTCC GAGGCGCCGG GACTACCTCC AGCACCGACT CGCTAGACAC CCTGAGCAAC 
GGACCGGAGG GCTGCGTGGT GGTGGCGCGC AGTGAGAAGG AGCAGCTGCA GGTGCTGAAC 
GACCGCTTCG CGGGCTACAT CGACAAGGTG CGGCAGCTCG AGGCGCATAA CCGCAGCCTG 
GAGGGCGAGG CGGCGGCGCT GCGGCAGCAG CAGGCGGGCC GCGCAGCCAT GGGCGAGCTG 
TACGAGCGCG AGGTGCGCGA GATGCGCGGC GCAGTGCTGC GCTTGGGCGC GGCTCGCGGC 
CAGCTGCGCC TGGAGCAGGA GCACCTGCTG GAGGACATCG CGCACGTGCG CCAGCGCCTG 
GACGACGAGG CCCGGCAGCG CGAGGAGGCC GAGGCAGCGA CGCGCGCACT GGCCCGCTTT 
GCGCAGGAGG CCGAGGCGGC GCGCGTCGAG CTGCAGAAGA AGGCACAAGC CCTGCAGGAA 
GAGTGCGGCT ACCTGCGGCG TCACCACCAG GAGGAGGTGG GCGAGCTGCT CGGCCAGATC 
CAGAGCAGCA GCGCCGCGCA GACGCAGGCC GAGGCGCGCG ATGCCCTCAA GTGCGACGTG 
ACGTCGGCCC TGCGCGAGAT CCGCGCGCAG CTTGAAGGCC ACACGGTGCA GAGCACGCTT 
CAGTCCGAGG AGTGGTTCCG AGTGAGGCTG GACCGACTGT CCGAGGCAGC CAAGGTGAAC 
ACAGACGCCA TGCGCTCAGC ACAGGAGGAG ATATCTGAGT ACCGGCGCCA GCTGCAGGCC 
AGGACCACAG AGCTGGAGAC GCTCAAAAGC ACCAAGGACT CACTGGAGAG GCAGCGCTCT 
GAGCTGGAGG ACCGTCACCA GGCTGACATC GCATCCTACC AGGAGGCCAT CCAGCAGCTG 
GATGCAGAGC TGAGGAACAC CAAGTGGGAG ATGGCAGCCC AGCTCCGAGA GTACCAGGAT 
CTACTCAATG TCAAGATGGC TCTGGACATT GAGATCGCTG CTTACAGAAA ACTCCTGGAG 
GGTGAAGAAT GTCGAATTGG CTTTGGCCCG AGTCCTTTCC TCCTTCCAGA AGGACTCCCC 
AAGATCCCCT CCGTGTCCAC TCACATCAAA GTCAAAAGTG AAGAGAAGAT CAAGATGGTA 
GAAAAGTCAG AGAAGGAAAC TGTGATTGTG GAGGAACAGA CAGAGGAGGT CCAAGTGACT 
GAAGAAGTGA CTGAAGAAGA AGAGAAAGAG GCCAAAGAGG AGGAAGGAGA AGCAGTAAAG 
TCTCCCCCAG CAGAAGAGGC TGCATCCCCA GAGAAGGAGG AGGCCAAGTC ACCAGCCGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAGG AGGCCAAGTC CCCACAGAAG 
GAAGAGGCCA AGTCACCAGC TGAGGTGAAG TCCCCTGAGA AGGCCAAGTC CCCCGTGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAAG AAGCCAAATC CCCAGAGAAG 
GCCAAGTCCC CAGTGGAGGC CAAGTCTCCA GAAAAGGCCA AGTCCCCCAT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGT CAAATCCCCA GTGGAGGCCA AATCCCCAGA GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GAGAAAGCCA AGTCCCCTGT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GTGGAAGCCA AGTCCCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCA GTGGAGGCCA AGTCGCCAGA GAAGGCCAAG 
TCTCCTGTGA AGGAGGAGGC CAAGTCCCCA GAGAAGGCCA AGTCTCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT GTGAAAGAGG AGGCCAAGTC CCCAGAGAAG 
GCCAAGTCTC CTGTGAAGGA GGAGGCCAAG TCGCCAGAGA AGGCCAAGTC TCCTGTGAAA 
GAGGAGGCCA AGTCCCCAGA GAAGGCCAAG TCTCCTGTGA AAGAGGAGGC CAAGTCCCCA 
GAGAAGGCCA AGTCCCCAGT AGAGGCCAAG TCCCCAGAGA AGGCCAAGTC TCCTGCGAAG 
GAGGAGGCCA AGTCGCCAGA GAAGGCCAAG TCTCCTGTGA AAGAGGAGGC CAAGTCCCCA 
GAGAAGGCCA AGTCTCCTGT GAAAGAGGAG GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT 
GCGAAGGAGG AGGCCAAGTC CCCAGAGAAG GCCAAGTCCC CAGAGAAGCC CAAAAGCCCT 
GTGAAGGAGG AGGCCAAGTC CCCAGAGAAG CCCAAAAGCC CTGTGAAGGA GGAGGCCAAG 
TCCCCAGAGA AGCCCAAAAG CCCTGTCAAG GAGGAGGCCA AATCCCCAGA GAAGGCCAAG 
TCTCCTGTGA AGGATGAGGC CAAGGCTCCT GGGAAGGAGG TCCCAAAGAA GGAGGAGGCC 
AAGTCCCCTG TGAAGGAGGA AGAAAAACCC CAGGAGGTGA GAGCCAAAGA GCCCCCAAAG 
AAGGCAGAGG AAGAGAAAGC TCCAGCCATA CCCAAAGCTG AGGAGAAGAA GGACAGCAAG 
AAAGATGAGG TGCCCAAGAA GGAGGCTCCA AAGCCCGAGG AGAAGAAGGA GCCTGCTGTG 
GAAAAGCCCA AAGAACCCAA AGCTGAAGCC AAGAAGGAAG AGGCTGAAGA TAAGAAAAAA 
GCAACCACCC CAGAGAAGGA GGCTCCTGCC AAGGGGAAGG AAGAGGCCAA GCCTAAAGAG 
AAGACTGAGG TGGCCAAGAA GGAACCAGAT GATGCCAAGC CCAAAGAACC CAGCAAAGCA 
GCAGAAAAGC CAAAGAAGGA AGAGACGCCG GCAGCACCGG AGAAAAAAGA CGCCAAGGAG 
GGGAAGGCCG CAGAAGCCAA GAAGCCTGAG GAGAAACCCA AAACAGAGGC CCAAGCCAAG 
GAACCTGGCA AGGAGGCCTC CACACCCGGG AAAGCCAAGG CTGAGAAATC CTCCAGCACA 
GACCAGAAAG ACAGCAGACC TGCAGAGAAG GCCGCAGAAG ATAAGGCCAC CAAGGGAGAG 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEFH ( XM_005218112.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005218169.1 Bos taurus neurofilament heavy (NEFH), transcript variant X1, mRNA.

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CloneID	OBa28068
Clone ID Related Accession (Same CDS sequence)	XM_005218112.2 , XM_005218112.3 , XM_005195129.3
Accession Version	XM_005218112.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	neurofilament heavy polypeptide isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005218112.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCA GCGGCGCCGA CGCGCTGCTG GGCGCCTTCG CGCCGCTCCA CGGAGGCGGC 
AGCTTGCACT ATGCACTGGC TCGCAAAGGC GGCGCCGGCG GAGCGCGCTC TGCAGCCGGC 
TCCTCCAGCG GATTCCACTC ATGGGCGCGG ACCTCCGTGA GCTCCGTGTC GGCCTCCCCG 
AGCCGCTTCC GAGGCGCCGG GACTACCTCC AGCACCGACT CGCTAGACAC CCTGAGCAAC 
GGACCGGAGG GCTGCGTGGT GGTGGCGCGC AGTGAGAAGG AGCAGCTGCA GGTGCTGAAC 
GACCGCTTCG CGGGCTACAT CGACAAGGTG CGGCAGCTCG AGGCGCATAA CCGCAGCCTG 
GAGGGCGAGG CGGCGGCGCT GCGGCAGCAG CAGGCGGGCC GCGCAGCCAT GGGCGAGCTG 
TACGAGCGCG AGGTGCGCGA GATGCGCGGC GCAGTGCTGC GCTTGGGCGC GGCTCGCGGC 
CAGCTGCGCC TGGAGCAGGA GCACCTGCTG GAGGACATCG CGCACGTGCG CCAGCGCCTG 
GACGACGAGG CCCGGCAGCG CGAGGAGGCC GAGGCAGCGA CGCGCGCACT GGCCCGCTTT 
GCGCAGGAGG CCGAGGCGGC GCGCGTCGAG CTGCAGAAGA AGGCACAAGC CCTGCAGGAA 
GAGTGCGGCT ACCTGCGGCG TCACCACCAG GAGGAGGTGG GCGAGCTGCT CGGCCAGATC 
CAGAGCAGCA GCGCCGCGCA GACGCAGGCC GAGGCGCGCG ATGCCCTCAA GTGCGACGTG 
ACGTCGGCCC TGCGCGAGAT CCGCGCGCAG CTTGAAGGCC ACACGGTGCA GAGCACGCTT 
CAGTCCGAGG AGTGGTTCCG AGTGAGGCTG GACCGACTGT CCGAGGCAGC CAAGGTGAAC 
ACAGACGCCA TGCGCTCAGC ACAGGAGGAG ATATCTGAGT ACCGGCGCCA GCTGCAGGCC 
AGGACCACAG AGCTGGAGAC GCTCAAAAGC ACCAAGGACT CACTGGAGAG GCAGCGCTCT 
GAGCTGGAGG ACCGTCACCA GGCTGACATC GCATCCTACC AGGAGGCCAT CCAGCAGCTG 
GATGCAGAGC TGAGGAACAC CAAGTGGGAG ATGGCAGCCC AGCTCCGAGA GTACCAGGAT 
CTACTCAATG TCAAGATGGC TCTGGACATT GAGATCGCTG CTTACAGAAA ACTCCTGGAG 
GGTGAAGAAT GTCGAATTGG CTTTGGCCCG AGTCCTTTCC TCCTTCCAGA AGGACTCCCC 
AAGATCCCCT CCGTGTCCAC TCACATCAAA GTCAAAAGTG AAGAGAAGAT CAAGATGGTA 
GAAAAGTCAG AGAAGGAAAC TGTGATTGTG GAGGAACAGA CAGAGGAGGT CCAAGTGACT 
GAAGAAGTGA CTGAAGAAGA AGAGAAAGAG GCCAAAGAGG AGGAAGGAGA AGCAGTAAAG 
TCTCCCCCAG CAGAAGAGGC TGCATCCCCA GAGAAGGAGG AGGCCAAGTC ACCAGCCGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAGG AGGCCAAGTC CCCACAGAAG 
GAAGAGGCCA AGTCACCAGC TGAGGTGAAG TCCCCTGAGA AGGCCAAGTC CCCCGTGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAAG AAGCCAAATC CCCAGAGAAG 
GCCAAGTCCC CAGTGGAGGC CAAGTCTCCA GAAAAGGCCA AGTCCCCCAT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGT CAAATCCCCA GTGGAGGCCA AATCCCCAGA GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GAGAAAGCCA AGTCCCCTGT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GTGGAAGCCA AGTCCCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCA GTGGAGGCCA AGTCGCCAGA GAAGGCCAAG 
TCTCCTGTGA AGGAGGAGGC CAAGTCCCCA GAGAAGGCCA AGTCTCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT GTGAAAGAGG AGGCCAAGTC CCCAGAGAAG 
GCCAAGTCTC CTGTGAAGGA GGAGGCCAAG TCGCCAGAGA AGGCCAAGTC TCCTGTGAAA 
GAGGAGGCCA AGTCCCCAGA GAAGGCCAAG TCTCCTGTGA AAGAGGAGGC CAAGTCCCCA 
GAGAAGGCCA AGTCCCCAGT AGAGGCCAAG TCCCCAGAGA AGGCCAAGTC TCCTGCGAAG 
GAGGAGGCCA AGTCGCCAGA GAAGGCCAAG TCTCCTGTGA AAGAGGAGGC CAAGTCCCCA 
GAGAAGGCCA AGTCTCCTGT GAAAGAGGAG GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT 
GCGAAGGAGG AGGCCAAGTC CCCAGAGAAG GCCAAGTCCC CAGAGAAGCC CAAAAGCCCT 
GTGAAGGAGG AGGCCAAGTC CCCAGAGAAG CCCAAAAGCC CTGTGAAGGA GGAGGCCAAG 
TCCCCAGAGA AGCCCAAAAG CCCTGTCAAG GAGGAGGCCA AATCCCCAGA GAAGGCCAAG 
TCTCCTGTGA AGGATGAGGC CAAGGCTCCT GGGAAGGAGG TCCCAAAGAA GGAGGAGGCC 
AAGTCCCCTG TGAAGGAGGA AGAAAAACCC CAGGAGGTGA GAGCCAAAGA GCCCCCAAAG 
AAGGCAGAGG AAGAGAAAGC TCCAGCCATA CCCAAAGCTG AGGAGAAGAA GGACAGCAAG 
AAAGATGAGG TGCCCAAGAA GGAGGCTCCA AAGCCCGAGG AGAAGAAGGA GCCTGCTGTG 
GAAAAGCCCA AAGAACCCAA AGCTGAAGCC AAGAAGGAAG AGGCTGAAGA TAAGAAAAAA 
GCAACCACCC CAGAGAAGGA GGCTCCTGCC AAGGGGAAGG AAGAGGCCAA GCCTAAAGAG 
AAGACTGAGG TGGCCAAGAA GGAACCAGAT GATGCCAAGC CCAAAGAACC CAGCAAAGCA 
GCAGAAAAGC CAAAGAAGGA AGAGACGCCG GCAGCACCGG AGAAAAAAGA CGCCAAGGAG 
GGGAAGGCCG CAGAAGCCAA GAAGCCTGAG GAGAAACCCA AAACAGAGGC CCAAGCCAAG 
GAACCTGGCA AGGAGGCCTC CACACCCGGG AAAGCCAAGG CTGAGAAATC CTCCAGCACA 
GACCAGAAAG ACAGCAGACC TGCAGAGAAG GCCGCAGAAG ATAAGGCCAC CAAGGGAGAG 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005218169.1
CDS	76..3321
Translation	MSFSGADALLGAFAPLHGGGSLHYALARKGGAGGARSAAGSSSGFHSWARTSVSSVSASPSRFRGAGTTSSTDSLDTLSNGPEGCVVVARSEKEQLQVLNDRFAGYIDKVRQLEAHNRSLEGEAAALRQQQAGRAAMGELYEREVREMRGAVLRLGAARGQLRLEQEHLLEDIAHVRQRLDDEARQREEAEAATRALARFAQEAEAARVELQKKAQALQEECGYLRRHHQEEVGELLGQIQSSSAAQTQAEARDALKCDVTSALREIRAQLEGHTVQSTLQSEEWFRVRLDRLSEAAKVNTDAMRSAQEEISEYRRQLQARTTELETLKSTKDSLERQRSELEDRHQADIASYQEAIQQLDAELRNTKWEMAAQLREYQDLLNVKMALDIEIAAYRKLLEGEECRIGFGPSPFLLPEGLPKIPSVSTHIKVKSEEKIKMVEKSEKETVIVEEQTEEVQVTEEVTEEEEKEAKEEEGEAVKSPPAEEAASPEKEEAKSPAEAKSPEKAKSPVKEEAKSPQKEEAKSPAEVKSPEKAKSPVEAKSPEKAKSPVKEEAKSPEKAKSPVEAKSPEKAKSPMKEEAKSPEKVKSPVEAKSPEKEEAKSPEKAKSPEKAKSPVKEEAKSPEKAKSPVEAKSPVKEEAKSPEKAKSPVEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVEAKSPEKAKSPAKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPAKEEAKSPEKAKSPEKPKSPVKEEAKSPEKPKSPVKEEAKSPEKPKSPVKEEAKSPEKAKSPVKDEAKAPGKEVPKKEEAKSPVKEEEKPQEVRAKEPPKKAEEEKAPAIPKAEEKKDSKKDEVPKKEAPKPEEKKEPAVEKPKEPKAEAKKEEAEDKKKATTPEKEAPAKGKEEAKPKEKTEVAKKEPDDAKPKEPSKAAEKPKKEETPAAPEKKDAKEGKAAEAKKPEEKPKTEAQAKEPGKEASTPGKAKAEKSSSTDQKDSRPAEKAAEDKATKGEK

Target ORF information:

RefSeq Version	XM_005218112.3
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofilament heavy (NEFH), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005218112.3

ORF Insert Sequence:

ATGAGCTTCA GCGGCGCCGA CGCGCTGCTG GGCGCCTTCG CGCCGCTCCA CGGAGGCGGC 
AGCTTGCACT ATGCACTGGC TCGCAAAGGC GGCGCCGGCG GAGCGCGCTC TGCAGCCGGC 
TCCTCCAGCG GATTCCACTC ATGGGCGCGG ACCTCCGTGA GCTCCGTGTC GGCCTCCCCG 
AGCCGCTTCC GAGGCGCCGG GACTACCTCC AGCACCGACT CGCTAGACAC CCTGAGCAAC 
GGACCGGAGG GCTGCGTGGT GGTGGCGCGC AGTGAGAAGG AGCAGCTGCA GGTGCTGAAC 
GACCGCTTCG CGGGCTACAT CGACAAGGTG CGGCAGCTCG AGGCGCATAA CCGCAGCCTG 
GAGGGCGAGG CGGCGGCGCT GCGGCAGCAG CAGGCGGGCC GCGCAGCCAT GGGCGAGCTG 
TACGAGCGCG AGGTGCGCGA GATGCGCGGC GCAGTGCTGC GCTTGGGCGC GGCTCGCGGC 
CAGCTGCGCC TGGAGCAGGA GCACCTGCTG GAGGACATCG CGCACGTGCG CCAGCGCCTG 
GACGACGAGG CCCGGCAGCG CGAGGAGGCC GAGGCAGCGA CGCGCGCACT GGCCCGCTTT 
GCGCAGGAGG CCGAGGCGGC GCGCGTCGAG CTGCAGAAGA AGGCACAAGC CCTGCAGGAA 
GAGTGCGGCT ACCTGCGGCG TCACCACCAG GAGGAGGTGG GCGAGCTGCT CGGCCAGATC 
CAGAGCAGCA GCGCCGCGCA GACGCAGGCC GAGGCGCGCG ATGCCCTCAA GTGCGACGTG 
ACGTCGGCCC TGCGCGAGAT CCGCGCGCAG CTTGAAGGCC ACACGGTGCA GAGCACGCTT 
CAGTCCGAGG AGTGGTTCCG AGTGAGGCTG GACCGACTGT CCGAGGCAGC CAAGGTGAAC 
ACAGACGCCA TGCGCTCAGC ACAGGAGGAG ATATCTGAGT ACCGGCGCCA GCTGCAGGCC 
AGGACCACAG AGCTGGAGAC GCTCAAAAGC ACCAAGGACT CACTGGAGAG GCAGCGCTCT 
GAGCTGGAGG ACCGTCACCA GGCTGACATC GCATCCTACC AGGAGGCCAT CCAGCAGCTG 
GATGCAGAGC TGAGGAACAC CAAGTGGGAG ATGGCAGCCC AGCTCCGAGA GTACCAGGAT 
CTACTCAATG TCAAGATGGC TCTGGACATT GAGATCGCTG CTTACAGAAA ACTCCTGGAG 
GGTGAAGAAT GTCGAATTGG CTTTGGCCCG AGTCCTTTCC TCCTTCCAGA AGGACTCCCC 
AAGATCCCCT CCGTGTCCAC TCACATCAAA GTCAAAAGTG AAGAGAAGAT CAAGATGGTA 
GAAAAGTCAG AGAAGGAAAC TGTGATTGTG GAGGAACAGA CAGAGGAGGT CCAAGTGACT 
GAAGAAGTGA CTGAAGAAGA AGAGAAAGAG GCCAAAGAGG AGGAAGGAGA AGCAGTAAAG 
TCTCCCCCAG CAGAAGAGGC TGCATCCCCA GAGAAGGAGG AGGCCAAGTC ACCAGCCGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAGG AGGCCAAGTC CCCACAGAAG 
GAAGAGGCCA AGTCACCAGC TGAGGTGAAG TCCCCTGAGA AGGCCAAGTC CCCCGTGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAAG AAGCCAAATC CCCAGAGAAG 
GCCAAGTCCC CAGTGGAGGC CAAGTCTCCA GAAAAGGCCA AGTCCCCCAT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGT CAAATCCCCA GTGGAGGCCA AATCCCCAGA GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GAGAAAGCCA AGTCCCCTGT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GTGGAAGCCA AGTCCCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCA GTGGAGGCCA AGTCGCCAGA GAAGGCCAAG 
TCTCCTGTGA AGGAGGAGGC CAAGTCCCCA GAGAAGGCCA AGTCTCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT GTGAAAGAGG AGGCCAAGTC CCCAGAGAAG 
GCCAAGTCTC CTGTGAAGGA GGAGGCCAAG TCGCCAGAGA AGGCCAAGTC TCCTGTGAAA 
GAGGAGGCCA AGTCCCCAGA GAAGGCCAAG TCTCCTGTGA AAGAGGAGGC CAAGTCCCCA 
GAGAAGGCCA AGTCCCCAGT AGAGGCCAAG TCCCCAGAGA AGGCCAAGTC TCCTGCGAAG 
GAGGAGGCCA AGTCGCCAGA GAAGGCCAAG TCTCCTGTGA AAGAGGAGGC CAAGTCCCCA 
GAGAAGGCCA AGTCTCCTGT GAAAGAGGAG GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT 
GCGAAGGAGG AGGCCAAGTC CCCAGAGAAG GCCAAGTCCC CAGAGAAGCC CAAAAGCCCT 
GTGAAGGAGG AGGCCAAGTC CCCAGAGAAG CCCAAAAGCC CTGTGAAGGA GGAGGCCAAG 
TCCCCAGAGA AGCCCAAAAG CCCTGTCAAG GAGGAGGCCA AATCCCCAGA GAAGGCCAAG 
TCTCCTGTGA AGGATGAGGC CAAGGCTCCT GGGAAGGAGG TCCCAAAGAA GGAGGAGGCC 
AAGTCCCCTG TGAAGGAGGA AGAAAAACCC CAGGAGGTGA GAGCCAAAGA GCCCCCAAAG 
AAGGCAGAGG AAGAGAAAGC TCCAGCCATA CCCAAAGCTG AGGAGAAGAA GGACAGCAAG 
AAAGATGAGG TGCCCAAGAA GGAGGCTCCA AAGCCCGAGG AGAAGAAGGA GCCTGCTGTG 
GAAAAGCCCA AAGAACCCAA AGCTGAAGCC AAGAAGGAAG AGGCTGAAGA TAAGAAAAAA 
GCAACCACCC CAGAGAAGGA GGCTCCTGCC AAGGGGAAGG AAGAGGCCAA GCCTAAAGAG 
AAGACTGAGG TGGCCAAGAA GGAACCAGAT GATGCCAAGC CCAAAGAACC CAGCAAAGCA 
GCAGAAAAGC CAAAGAAGGA AGAGACGCCG GCAGCACCGG AGAAAAAAGA CGCCAAGGAG 
GGGAAGGCCG CAGAAGCCAA GAAGCCTGAG GAGAAACCCA AAACAGAGGC CCAAGCCAAG 
GAACCTGGCA AGGAGGCCTC CACACCCGGG AAAGCCAAGG CTGAGAAATC CTCCAGCACA 
GACCAGAAAG ACAGCAGACC TGCAGAGAAG GCCGCAGAAG ATAAGGCCAC CAAGGGAGAG 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEFH ( XM_005195129.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005195186.2 Bos taurus neurofilament, heavy polypeptide (NEFH), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa28068
Clone ID Related Accession (Same CDS sequence)	XM_005218112.2 , XM_005218112.3 , XM_005195129.3
Accession Version	XM_005195129.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neurofilament heavy polypeptide isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646373.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005195129.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCA GCGGCGCCGA CGCGCTGCTG GGCGCCTTCG CGCCGCTCCA CGGAGGCGGC 
AGCTTGCACT ATGCACTGGC TCGCAAAGGC GGCGCCGGCG GAGCGCGCTC TGCAGCCGGC 
TCCTCCAGCG GATTCCACTC ATGGGCGCGG ACCTCCGTGA GCTCCGTGTC GGCCTCCCCG 
AGCCGCTTCC GAGGCGCCGG GACTACCTCC AGCACCGACT CGCTAGACAC CCTGAGCAAC 
GGACCGGAGG GCTGCGTGGT GGTGGCGCGC AGTGAGAAGG AGCAGCTGCA GGTGCTGAAC 
GACCGCTTCG CGGGCTACAT CGACAAGGTG CGGCAGCTCG AGGCGCATAA CCGCAGCCTG 
GAGGGCGAGG CGGCGGCGCT GCGGCAGCAG CAGGCGGGCC GCGCAGCCAT GGGCGAGCTG 
TACGAGCGCG AGGTGCGCGA GATGCGCGGC GCAGTGCTGC GCTTGGGCGC GGCTCGCGGC 
CAGCTGCGCC TGGAGCAGGA GCACCTGCTG GAGGACATCG CGCACGTGCG CCAGCGCCTG 
GACGACGAGG CCCGGCAGCG CGAGGAGGCC GAGGCAGCGA CGCGCGCACT GGCCCGCTTT 
GCGCAGGAGG CCGAGGCGGC GCGCGTCGAG CTGCAGAAGA AGGCACAAGC CCTGCAGGAA 
GAGTGCGGCT ACCTGCGGCG TCACCACCAG GAGGAGGTGG GCGAGCTGCT CGGCCAGATC 
CAGAGCAGCA GCGCCGCGCA GACGCAGGCC GAGGCGCGCG ATGCCCTCAA GTGCGACGTG 
ACGTCGGCCC TGCGCGAGAT CCGCGCGCAG CTTGAAGGCC ACACGGTGCA GAGCACGCTT 
CAGTCCGAGG AGTGGTTCCG AGTGAGGCTG GACCGACTGT CCGAGGCAGC CAAGGTGAAC 
ACAGACGCCA TGCGCTCAGC ACAGGAGGAG ATATCTGAGT ACCGGCGCCA GCTGCAGGCC 
AGGACCACAG AGCTGGAGAC GCTCAAAAGC ACCAAGGACT CACTGGAGAG GCAGCGCTCT 
GAGCTGGAGG ACCGTCACCA GGCTGACATC GCATCCTACC AGGAGGCCAT CCAGCAGCTG 
GATGCAGAGC TGAGGAACAC CAAGTGGGAG ATGGCAGCCC AGCTCCGAGA GTACCAGGAT 
CTACTCAATG TCAAGATGGC TCTGGACATT GAGATCGCTG CTTACAGAAA ACTCCTGGAG 
GGTGAAGAAT GTCGAATTGG CTTTGGCCCG AGTCCTTTCC TCCTTCCAGA AGGACTCCCC 
AAGATCCCCT CCGTGTCCAC TCACATCAAA GTCAAAAGTG AAGAGAAGAT CAAGATGGTA 
GAAAAGTCAG AGAAGGAAAC TGTGATTGTG GAGGAACAGA CAGAGGAGGT CCAAGTGACT 
GAAGAAGTGA CTGAAGAAGA AGAGAAAGAG GCCAAAGAGG AGGAAGGAGA AGCAGTAAAG 
TCTCCCCCAG CAGAAGAGGC TGCATCCCCA GAGAAGGAGG AGGCCAAGTC ACCAGCCGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAGG AGGCCAAGTC CCCACAGAAG 
GAAGAGGCCA AGTCACCAGC TGAGGTGAAG TCCCCTGAGA AGGCCAAGTC CCCCGTGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAAG AAGCCAAATC CCCAGAGAAG 
GCCAAGTCCC CAGTGGAGGC CAAGTCTCCA GAAAAGGCCA AGTCCCCCAT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGT CAAATCCCCA GTGGAGGCCA AATCCCCAGA GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GAGAAAGCCA AGTCCCCTGT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GTGGAAGCCA AGTCCCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCA GTGGAGGCCA AGTCGCCAGA GAAGGCCAAG 
TCTCCTGTGA AGGAGGAGGC CAAGTCCCCA GAGAAGGCCA AGTCTCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT GTGAAAGAGG AGGCCAAGTC CCCAGAGAAG 
GCCAAGTCTC CTGTGAAGGA GGAGGCCAAG TCGCCAGAGA AGGCCAAGTC TCCTGTGAAA 
GAGGAGGCCA AGTCCCCAGA GAAGGCCAAG TCTCCTGTGA AAGAGGAGGC CAAGTCCCCA 
GAGAAGGCCA AGTCCCCAGT AGAGGCCAAG TCCCCAGAGA AGGCCAAGTC TCCTGCGAAG 
GAGGAGGCCA AGTCGCCAGA GAAGGCCAAG TCTCCTGTGA AAGAGGAGGC CAAGTCCCCA 
GAGAAGGCCA AGTCTCCTGT GAAAGAGGAG GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT 
GCGAAGGAGG AGGCCAAGTC CCCAGAGAAG GCCAAGTCCC CAGAGAAGCC CAAAAGCCCT 
GTGAAGGAGG AGGCCAAGTC CCCAGAGAAG CCCAAAAGCC CTGTGAAGGA GGAGGCCAAG 
TCCCCAGAGA AGCCCAAAAG CCCTGTCAAG GAGGAGGCCA AATCCCCAGA GAAGGCCAAG 
TCTCCTGTGA AGGATGAGGC CAAGGCTCCT GGGAAGGAGG TCCCAAAGAA GGAGGAGGCC 
AAGTCCCCTG TGAAGGAGGA AGAAAAACCC CAGGAGGTGA GAGCCAAAGA GCCCCCAAAG 
AAGGCAGAGG AAGAGAAAGC TCCAGCCATA CCCAAAGCTG AGGAGAAGAA GGACAGCAAG 
AAAGATGAGG TGCCCAAGAA GGAGGCTCCA AAGCCCGAGG AGAAGAAGGA GCCTGCTGTG 
GAAAAGCCCA AAGAACCCAA AGCTGAAGCC AAGAAGGAAG AGGCTGAAGA TAAGAAAAAA 
GCAACCACCC CAGAGAAGGA GGCTCCTGCC AAGGGGAAGG AAGAGGCCAA GCCTAAAGAG 
AAGACTGAGG TGGCCAAGAA GGAACCAGAT GATGCCAAGC CCAAAGAACC CAGCAAAGCA 
GCAGAAAAGC CAAAGAAGGA AGAGACGCCG GCAGCACCGG AGAAAAAAGA CGCCAAGGAG 
GGGAAGGCCG CAGAAGCCAA GAAGCCTGAG GAGAAACCCA AAACAGAGGC CCAAGCCAAG 
GAACCTGGCA AGGAGGCCTC CACACCCGGG AAAGCCAAGG CTGAGAAATC CTCCAGCACA 
GACCAGAAAG ACAGCAGACC TGCAGAGAAG GCCGCAGAAG ATAAGGCCAC CAAGGGAGAG 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005195186.2
CDS	79..3324
Translation	MSFSGADALLGAFAPLHGGGSLHYALARKGGAGGARSAAGSSSGFHSWARTSVSSVSASPSRFRGAGTTSSTDSLDTLSNGPEGCVVVARSEKEQLQVLNDRFAGYIDKVRQLEAHNRSLEGEAAALRQQQAGRAAMGELYEREVREMRGAVLRLGAARGQLRLEQEHLLEDIAHVRQRLDDEARQREEAEAATRALARFAQEAEAARVELQKKAQALQEECGYLRRHHQEEVGELLGQIQSSSAAQTQAEARDALKCDVTSALREIRAQLEGHTVQSTLQSEEWFRVRLDRLSEAAKVNTDAMRSAQEEISEYRRQLQARTTELETLKSTKDSLERQRSELEDRHQADIASYQEAIQQLDAELRNTKWEMAAQLREYQDLLNVKMALDIEIAAYRKLLEGEECRIGFGPSPFLLPEGLPKIPSVSTHIKVKSEEKIKMVEKSEKETVIVEEQTEEVQVTEEVTEEEEKEAKEEEGEAVKSPPAEEAASPEKEEAKSPAEAKSPEKAKSPVKEEAKSPQKEEAKSPAEVKSPEKAKSPVEAKSPEKAKSPVKEEAKSPEKAKSPVEAKSPEKAKSPMKEEAKSPEKVKSPVEAKSPEKEEAKSPEKAKSPEKAKSPVKEEAKSPEKAKSPVEAKSPVKEEAKSPEKAKSPVEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVEAKSPEKAKSPAKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPAKEEAKSPEKAKSPEKPKSPVKEEAKSPEKPKSPVKEEAKSPEKPKSPVKEEAKSPEKAKSPVKDEAKAPGKEVPKKEEAKSPVKEEEKPQEVRAKEPPKKAEEEKAPAIPKAEEKKDSKKDEVPKKEAPKPEEKKEPAVEKPKEPKAEAKKEEAEDKKKATTPEKEAPAKGKEEAKPKEKTEVAKKEPDDAKPKEPSKAAEKPKKEETPAAPEKKDAKEGKAAEAKKPEEKPKTEAQAKEPGKEASTPGKAKAEKSSSTDQKDSRPAEKAAEDKATKGEK

Target ORF information:

RefSeq Version	XM_005195129.3
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofilament, heavy polypeptide (NEFH), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005195129.3

ORF Insert Sequence:

ATGAGCTTCA GCGGCGCCGA CGCGCTGCTG GGCGCCTTCG CGCCGCTCCA CGGAGGCGGC 
AGCTTGCACT ATGCACTGGC TCGCAAAGGC GGCGCCGGCG GAGCGCGCTC TGCAGCCGGC 
TCCTCCAGCG GATTCCACTC ATGGGCGCGG ACCTCCGTGA GCTCCGTGTC GGCCTCCCCG 
AGCCGCTTCC GAGGCGCCGG GACTACCTCC AGCACCGACT CGCTAGACAC CCTGAGCAAC 
GGACCGGAGG GCTGCGTGGT GGTGGCGCGC AGTGAGAAGG AGCAGCTGCA GGTGCTGAAC 
GACCGCTTCG CGGGCTACAT CGACAAGGTG CGGCAGCTCG AGGCGCATAA CCGCAGCCTG 
GAGGGCGAGG CGGCGGCGCT GCGGCAGCAG CAGGCGGGCC GCGCAGCCAT GGGCGAGCTG 
TACGAGCGCG AGGTGCGCGA GATGCGCGGC GCAGTGCTGC GCTTGGGCGC GGCTCGCGGC 
CAGCTGCGCC TGGAGCAGGA GCACCTGCTG GAGGACATCG CGCACGTGCG CCAGCGCCTG 
GACGACGAGG CCCGGCAGCG CGAGGAGGCC GAGGCAGCGA CGCGCGCACT GGCCCGCTTT 
GCGCAGGAGG CCGAGGCGGC GCGCGTCGAG CTGCAGAAGA AGGCACAAGC CCTGCAGGAA 
GAGTGCGGCT ACCTGCGGCG TCACCACCAG GAGGAGGTGG GCGAGCTGCT CGGCCAGATC 
CAGAGCAGCA GCGCCGCGCA GACGCAGGCC GAGGCGCGCG ATGCCCTCAA GTGCGACGTG 
ACGTCGGCCC TGCGCGAGAT CCGCGCGCAG CTTGAAGGCC ACACGGTGCA GAGCACGCTT 
CAGTCCGAGG AGTGGTTCCG AGTGAGGCTG GACCGACTGT CCGAGGCAGC CAAGGTGAAC 
ACAGACGCCA TGCGCTCAGC ACAGGAGGAG ATATCTGAGT ACCGGCGCCA GCTGCAGGCC 
AGGACCACAG AGCTGGAGAC GCTCAAAAGC ACCAAGGACT CACTGGAGAG GCAGCGCTCT 
GAGCTGGAGG ACCGTCACCA GGCTGACATC GCATCCTACC AGGAGGCCAT CCAGCAGCTG 
GATGCAGAGC TGAGGAACAC CAAGTGGGAG ATGGCAGCCC AGCTCCGAGA GTACCAGGAT 
CTACTCAATG TCAAGATGGC TCTGGACATT GAGATCGCTG CTTACAGAAA ACTCCTGGAG 
GGTGAAGAAT GTCGAATTGG CTTTGGCCCG AGTCCTTTCC TCCTTCCAGA AGGACTCCCC 
AAGATCCCCT CCGTGTCCAC TCACATCAAA GTCAAAAGTG AAGAGAAGAT CAAGATGGTA 
GAAAAGTCAG AGAAGGAAAC TGTGATTGTG GAGGAACAGA CAGAGGAGGT CCAAGTGACT 
GAAGAAGTGA CTGAAGAAGA AGAGAAAGAG GCCAAAGAGG AGGAAGGAGA AGCAGTAAAG 
TCTCCCCCAG CAGAAGAGGC TGCATCCCCA GAGAAGGAGG AGGCCAAGTC ACCAGCCGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAGG AGGCCAAGTC CCCACAGAAG 
GAAGAGGCCA AGTCACCAGC TGAGGTGAAG TCCCCTGAGA AGGCCAAGTC CCCCGTGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAAG AAGCCAAATC CCCAGAGAAG 
GCCAAGTCCC CAGTGGAGGC CAAGTCTCCA GAAAAGGCCA AGTCCCCCAT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGT CAAATCCCCA GTGGAGGCCA AATCCCCAGA GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GAGAAAGCCA AGTCCCCTGT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GTGGAAGCCA AGTCCCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCA GTGGAGGCCA AGTCGCCAGA GAAGGCCAAG 
TCTCCTGTGA AGGAGGAGGC CAAGTCCCCA GAGAAGGCCA AGTCTCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT GTGAAAGAGG AGGCCAAGTC CCCAGAGAAG 
GCCAAGTCTC CTGTGAAGGA GGAGGCCAAG TCGCCAGAGA AGGCCAAGTC TCCTGTGAAA 
GAGGAGGCCA AGTCCCCAGA GAAGGCCAAG TCTCCTGTGA AAGAGGAGGC CAAGTCCCCA 
GAGAAGGCCA AGTCCCCAGT AGAGGCCAAG TCCCCAGAGA AGGCCAAGTC TCCTGCGAAG 
GAGGAGGCCA AGTCGCCAGA GAAGGCCAAG TCTCCTGTGA AAGAGGAGGC CAAGTCCCCA 
GAGAAGGCCA AGTCTCCTGT GAAAGAGGAG GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT 
GCGAAGGAGG AGGCCAAGTC CCCAGAGAAG GCCAAGTCCC CAGAGAAGCC CAAAAGCCCT 
GTGAAGGAGG AGGCCAAGTC CCCAGAGAAG CCCAAAAGCC CTGTGAAGGA GGAGGCCAAG 
TCCCCAGAGA AGCCCAAAAG CCCTGTCAAG GAGGAGGCCA AATCCCCAGA GAAGGCCAAG 
TCTCCTGTGA AGGATGAGGC CAAGGCTCCT GGGAAGGAGG TCCCAAAGAA GGAGGAGGCC 
AAGTCCCCTG TGAAGGAGGA AGAAAAACCC CAGGAGGTGA GAGCCAAAGA GCCCCCAAAG 
AAGGCAGAGG AAGAGAAAGC TCCAGCCATA CCCAAAGCTG AGGAGAAGAA GGACAGCAAG 
AAAGATGAGG TGCCCAAGAA GGAGGCTCCA AAGCCCGAGG AGAAGAAGGA GCCTGCTGTG 
GAAAAGCCCA AAGAACCCAA AGCTGAAGCC AAGAAGGAAG AGGCTGAAGA TAAGAAAAAA 
GCAACCACCC CAGAGAAGGA GGCTCCTGCC AAGGGGAAGG AAGAGGCCAA GCCTAAAGAG 
AAGACTGAGG TGGCCAAGAA GGAACCAGAT GATGCCAAGC CCAAAGAACC CAGCAAAGCA 
GCAGAAAAGC CAAAGAAGGA AGAGACGCCG GCAGCACCGG AGAAAAAAGA CGCCAAGGAG 
GGGAAGGCCG CAGAAGCCAA GAAGCCTGAG GAGAAACCCA AAACAGAGGC CCAAGCCAAG 
GAACCTGGCA AGGAGGCCTC CACACCCGGG AAAGCCAAGG CTGAGAAATC CTCCAGCACA 
GACCAGAAAG ACAGCAGACC TGCAGAGAAG GCCGCAGAAG ATAAGGCCAC CAAGGGAGAG 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEFH ( XM_010823474.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010821776.2 Bos taurus neurofilament, heavy polypeptide (NEFH), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa28070
Clone ID Related Accession (Same CDS sequence)	XM_010823474.2 , XM_005218114.3 , XM_005218114.2
Accession Version	XM_010823474.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neurofilament heavy polypeptide isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646373.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010823474.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCA GCGGCGCCGA CGCGCTGCTG GGCGCCTTCG CGCCGCTCCA CGGAGGCGGC 
AGCTTGCACT ATGCACTGGC TCGCAAAGGC GGCGCCGGCG GAGCGCGCTC TGCAGCCGGC 
TCCTCCAGCG GATTCCACTC ATGGGCGCGG ACCTCCGTGA GCTCCGTGTC GGCCTCCCCG 
AGCCGCTTCC GAGGCGCCGG GACTACCTCC AGCACCGACT CGCTAGACAC CCTGAGCAAC 
GGACCGGAGG GCTGCGTGGT GGTGGCGCGC AGTGAGAAGG AGCAGCTGCA GGTGCTGAAC 
GACCGCTTCG CGGGCTACAT CGACAAGGTG CGGCAGCTCG AGGCGCATAA CCGCAGCCTG 
GAGGGCGAGG CGGCGGCGCT GCGGCAGCAG CAGGCGGGCC GCGCAGCCAT GGGCGAGCTG 
TACGAGCGCG AGGTGCGCGA GATGCGCGGC GCAGTGCTGC GCTTGGGCGC GGCTCGCGGC 
CAGCTGCGCC TGGAGCAGGA GCACCTGCTG GAGGACATCG CGCACGTGCG CCAGCGCCTG 
GACGACGAGG CCCGGCAGCG CGAGGAGGCC GAGGCAGCGA CGCGCGCACT GGCCCGCTTT 
GCGCAGGAGG CCGAGGCGGC GCGCGTCGAG CTGCAGAAGA AGGCACAAGC CCTGCAGGAA 
GAGTGCGGCT ACCTGCGGCG TCACCACCAG GAGGAGGTGG GCGAGCTGCT CGGCCAGATC 
CAGAGCAGCA GCGCCGCGCA GACGCAGGCC GAGGCGCGCG ATGCCCTCAA GTGCGACGTG 
ACGTCGGCCC TGCGCGAGAT CCGCGCGCAG CTTGAAGGCC ACACGGTGCA GAGCACGCTT 
CAGTCCGAGG AGTGGTTCCG AGTGAGGCTG GACCGACTGT CCGAGGCAGC CAAGGTGAAC 
ACAGACGCCA TGCGCTCAGC ACAGGAGGAG ATATCTGAGT ACCGGCGCCA GCTGCAGGCC 
AGGACCACAG AGCTGGAGAC GCTCAAAAGC ACCAAGGACT CACTGGAGAG GCAGCGCTCT 
GAGCTGGAGG ACCGTCACCA GGCTGACATC GCATCCTACC AGGAGGCCAT CCAGCAGCTG 
GATGCAGAGC TGAGGAACAC CAAGTGGGAG ATGGCAGCCC AGCTCCGAGA GTACCAGGAT 
CTACTCAATG TCAAGATGGC TCTGGACATT GAGATCGCTG CTTACAGAAA ACTCCTGGAG 
GGTGAAGAAT GTCGAATTGG CTTTGGCCCG AGTCCTTTCC TCCTTCCAGA AGGACTCCCC 
AAGATCCCCT CCGTGTCCAC TCACATCAAA GTCAAAAGTG AAGAGAAGAT CAAGATGGTA 
GAAAAGTCAG AGAAGGAAAC TGTGATTGTG GAGGAACAGA CAGAGGAGGT CCAAGTGACT 
GAAGAAGTGA CTGAAGAAGA AGAGAAAGAG GCCAAAGAGG AGGAAGGAGA AGCAGTAAAG 
TCTCCCCCAG CAGAAGAGGC TGCATCCCCA GAGAAGGAGG AGGCCAAGTC ACCAGCCGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAGG AGGCCAAGTC CCCACAGAAG 
GAAGAGGCCA AGTCACCAGC TGAGGTGAAG TCCCCTGAGA AGGCCAAGTC CCCCGTGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAAG AAGCCAAATC CCCAGAGAAG 
GCCAAGTCCC CAGTGGAGGC CAAGTCTCCA GAAAAGGCCA AGTCCCCCAT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGT CAAATCCCCA GTGGAGGCCA AATCCCCAGA GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GAGAAAGCCA AGTCCCCTGT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GTGGAAGCCA AGTCCCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCA GTGGAGGCCA AGTCGCCAGA GAAGGCCAAG 
TCTCCTGTGA AGGAGGAGGC CAAGTCCCCA GAGAAGGCCA AGTCTCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT GTGAAAGAGG AGGCCAAGTC CCCAGAGAAG 
GCCAAGTCTC CTGTGAAGGA GGAGGCCAAG TCGCCAGAGA AGGCCAAGTC TCCTGTGAAA 
GAGGAGGCCA AGTCCCCAGA GAAGGCCAAG TCTCCTGTGA AAGAGGAGGC CAAGTCCCCA 
GAGAAGGCCA AGTCTCCTGC GAAGGAGGAG GCCAAGTCGC CAGAGAAGGC CAAGTCTCCT 
GTGAAAGAGG AGGCCAAGTC CCCAGAGAAG GCCAAGTCTC CTGTGAAAGA GGAGGCCAAG 
TCCCCAGAGA AGGCCAAGTC TCCTGCGAAG GAGGAGGCCA AGTCCCCAGA GAAGGCCAAG 
TCCCCAGAGA AGCCCAAAAG CCCTGTGAAG GAGGAGGCCA AGTCCCCAGA GAAGCCCAAA 
AGCCCTGTGA AGGAGGAGGC CAAGTCCCCA GAGAAGCCCA AAAGCCCTGT CAAGGAGGAG 
GCCAAATCCC CAGAGAAGGC CAAGTCTCCT GTGAAGGATG AGGCCAAGGC TCCTGGGAAG 
GAGGTCCCAA AGAAGGAGGA GGCCAAGTCC CCTGTGAAGG AGGAAGAAAA ACCCCAGGAG 
GTGAGAGCCA AAGAGCCCCC AAAGAAGGCA GAGGAAGAGA AAGCTCCAGC CATACCCAAA 
GCTGAGGAGA AGAAGGACAG CAAGAAAGAT GAGGTGCCCA AGAAGGAGGC TCCAAAGCCC 
GAGGAGAAGA AGGAGCCTGC TGTGGAAAAG CCCAAAGAAC CCAAAGCTGA AGCCAAGAAG 
GAAGAGGCTG AAGATAAGAA AAAAGCAACC ACCCCAGAGA AGGAGGCTCC TGCCAAGGGG 
AAGGAAGAGG CCAAGCCTAA AGAGAAGACT GAGGTGGCCA AGAAGGAACC AGATGATGCC 
AAGCCCAAAG AACCCAGCAA AGCAGCAGAA AAGCCAAAGA AGGAAGAGAC GCCGGCAGCA 
CCGGAGAAAA AAGACGCCAA GGAGGGGAAG GCCGCAGAAG CCAAGAAGCC TGAGGAGAAA 
CCCAAAACAG AGGCCCAAGC CAAGGAACCT GGCAAGGAGG CCTCCACACC CGGGAAAGCC 
AAGGCTGAGA AATCCTCCAG CACAGACCAG AAAGACAGCA GACCTGCAGA GAAGGCCGCA 
GAAGATAAGG CCACCAAGGG AGAGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010821776.2
CDS	79..3288
Translation	MSFSGADALLGAFAPLHGGGSLHYALARKGGAGGARSAAGSSSGFHSWARTSVSSVSASPSRFRGAGTTSSTDSLDTLSNGPEGCVVVARSEKEQLQVLNDRFAGYIDKVRQLEAHNRSLEGEAAALRQQQAGRAAMGELYEREVREMRGAVLRLGAARGQLRLEQEHLLEDIAHVRQRLDDEARQREEAEAATRALARFAQEAEAARVELQKKAQALQEECGYLRRHHQEEVGELLGQIQSSSAAQTQAEARDALKCDVTSALREIRAQLEGHTVQSTLQSEEWFRVRLDRLSEAAKVNTDAMRSAQEEISEYRRQLQARTTELETLKSTKDSLERQRSELEDRHQADIASYQEAIQQLDAELRNTKWEMAAQLREYQDLLNVKMALDIEIAAYRKLLEGEECRIGFGPSPFLLPEGLPKIPSVSTHIKVKSEEKIKMVEKSEKETVIVEEQTEEVQVTEEVTEEEEKEAKEEEGEAVKSPPAEEAASPEKEEAKSPAEAKSPEKAKSPVKEEAKSPQKEEAKSPAEVKSPEKAKSPVEAKSPEKAKSPVKEEAKSPEKAKSPVEAKSPEKAKSPMKEEAKSPEKVKSPVEAKSPEKEEAKSPEKAKSPEKAKSPVKEEAKSPEKAKSPVEAKSPVKEEAKSPEKAKSPVEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPAKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPAKEEAKSPEKAKSPEKPKSPVKEEAKSPEKPKSPVKEEAKSPEKPKSPVKEEAKSPEKAKSPVKDEAKAPGKEVPKKEEAKSPVKEEEKPQEVRAKEPPKKAEEEKAPAIPKAEEKKDSKKDEVPKKEAPKPEEKKEPAVEKPKEPKAEAKKEEAEDKKKATTPEKEAPAKGKEEAKPKEKTEVAKKEPDDAKPKEPSKAAEKPKKEETPAAPEKKDAKEGKAAEAKKPEEKPKTEAQAKEPGKEASTPGKAKAEKSSSTDQKDSRPAEKAAEDKATKGEK

Target ORF information:

RefSeq Version	XM_010823474.2
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofilament, heavy polypeptide (NEFH), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010823474.2

ORF Insert Sequence:

ATGAGCTTCA GCGGCGCCGA CGCGCTGCTG GGCGCCTTCG CGCCGCTCCA CGGAGGCGGC 
AGCTTGCACT ATGCACTGGC TCGCAAAGGC GGCGCCGGCG GAGCGCGCTC TGCAGCCGGC 
TCCTCCAGCG GATTCCACTC ATGGGCGCGG ACCTCCGTGA GCTCCGTGTC GGCCTCCCCG 
AGCCGCTTCC GAGGCGCCGG GACTACCTCC AGCACCGACT CGCTAGACAC CCTGAGCAAC 
GGACCGGAGG GCTGCGTGGT GGTGGCGCGC AGTGAGAAGG AGCAGCTGCA GGTGCTGAAC 
GACCGCTTCG CGGGCTACAT CGACAAGGTG CGGCAGCTCG AGGCGCATAA CCGCAGCCTG 
GAGGGCGAGG CGGCGGCGCT GCGGCAGCAG CAGGCGGGCC GCGCAGCCAT GGGCGAGCTG 
TACGAGCGCG AGGTGCGCGA GATGCGCGGC GCAGTGCTGC GCTTGGGCGC GGCTCGCGGC 
CAGCTGCGCC TGGAGCAGGA GCACCTGCTG GAGGACATCG CGCACGTGCG CCAGCGCCTG 
GACGACGAGG CCCGGCAGCG CGAGGAGGCC GAGGCAGCGA CGCGCGCACT GGCCCGCTTT 
GCGCAGGAGG CCGAGGCGGC GCGCGTCGAG CTGCAGAAGA AGGCACAAGC CCTGCAGGAA 
GAGTGCGGCT ACCTGCGGCG TCACCACCAG GAGGAGGTGG GCGAGCTGCT CGGCCAGATC 
CAGAGCAGCA GCGCCGCGCA GACGCAGGCC GAGGCGCGCG ATGCCCTCAA GTGCGACGTG 
ACGTCGGCCC TGCGCGAGAT CCGCGCGCAG CTTGAAGGCC ACACGGTGCA GAGCACGCTT 
CAGTCCGAGG AGTGGTTCCG AGTGAGGCTG GACCGACTGT CCGAGGCAGC CAAGGTGAAC 
ACAGACGCCA TGCGCTCAGC ACAGGAGGAG ATATCTGAGT ACCGGCGCCA GCTGCAGGCC 
AGGACCACAG AGCTGGAGAC GCTCAAAAGC ACCAAGGACT CACTGGAGAG GCAGCGCTCT 
GAGCTGGAGG ACCGTCACCA GGCTGACATC GCATCCTACC AGGAGGCCAT CCAGCAGCTG 
GATGCAGAGC TGAGGAACAC CAAGTGGGAG ATGGCAGCCC AGCTCCGAGA GTACCAGGAT 
CTACTCAATG TCAAGATGGC TCTGGACATT GAGATCGCTG CTTACAGAAA ACTCCTGGAG 
GGTGAAGAAT GTCGAATTGG CTTTGGCCCG AGTCCTTTCC TCCTTCCAGA AGGACTCCCC 
AAGATCCCCT CCGTGTCCAC TCACATCAAA GTCAAAAGTG AAGAGAAGAT CAAGATGGTA 
GAAAAGTCAG AGAAGGAAAC TGTGATTGTG GAGGAACAGA CAGAGGAGGT CCAAGTGACT 
GAAGAAGTGA CTGAAGAAGA AGAGAAAGAG GCCAAAGAGG AGGAAGGAGA AGCAGTAAAG 
TCTCCCCCAG CAGAAGAGGC TGCATCCCCA GAGAAGGAGG AGGCCAAGTC ACCAGCCGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAGG AGGCCAAGTC CCCACAGAAG 
GAAGAGGCCA AGTCACCAGC TGAGGTGAAG TCCCCTGAGA AGGCCAAGTC CCCCGTGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAAG AAGCCAAATC CCCAGAGAAG 
GCCAAGTCCC CAGTGGAGGC CAAGTCTCCA GAAAAGGCCA AGTCCCCCAT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGT CAAATCCCCA GTGGAGGCCA AATCCCCAGA GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GAGAAAGCCA AGTCCCCTGT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GTGGAAGCCA AGTCCCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCA GTGGAGGCCA AGTCGCCAGA GAAGGCCAAG 
TCTCCTGTGA AGGAGGAGGC CAAGTCCCCA GAGAAGGCCA AGTCTCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT GTGAAAGAGG AGGCCAAGTC CCCAGAGAAG 
GCCAAGTCTC CTGTGAAGGA GGAGGCCAAG TCGCCAGAGA AGGCCAAGTC TCCTGTGAAA 
GAGGAGGCCA AGTCCCCAGA GAAGGCCAAG TCTCCTGTGA AAGAGGAGGC CAAGTCCCCA 
GAGAAGGCCA AGTCTCCTGC GAAGGAGGAG GCCAAGTCGC CAGAGAAGGC CAAGTCTCCT 
GTGAAAGAGG AGGCCAAGTC CCCAGAGAAG GCCAAGTCTC CTGTGAAAGA GGAGGCCAAG 
TCCCCAGAGA AGGCCAAGTC TCCTGCGAAG GAGGAGGCCA AGTCCCCAGA GAAGGCCAAG 
TCCCCAGAGA AGCCCAAAAG CCCTGTGAAG GAGGAGGCCA AGTCCCCAGA GAAGCCCAAA 
AGCCCTGTGA AGGAGGAGGC CAAGTCCCCA GAGAAGCCCA AAAGCCCTGT CAAGGAGGAG 
GCCAAATCCC CAGAGAAGGC CAAGTCTCCT GTGAAGGATG AGGCCAAGGC TCCTGGGAAG 
GAGGTCCCAA AGAAGGAGGA GGCCAAGTCC CCTGTGAAGG AGGAAGAAAA ACCCCAGGAG 
GTGAGAGCCA AAGAGCCCCC AAAGAAGGCA GAGGAAGAGA AAGCTCCAGC CATACCCAAA 
GCTGAGGAGA AGAAGGACAG CAAGAAAGAT GAGGTGCCCA AGAAGGAGGC TCCAAAGCCC 
GAGGAGAAGA AGGAGCCTGC TGTGGAAAAG CCCAAAGAAC CCAAAGCTGA AGCCAAGAAG 
GAAGAGGCTG AAGATAAGAA AAAAGCAACC ACCCCAGAGA AGGAGGCTCC TGCCAAGGGG 
AAGGAAGAGG CCAAGCCTAA AGAGAAGACT GAGGTGGCCA AGAAGGAACC AGATGATGCC 
AAGCCCAAAG AACCCAGCAA AGCAGCAGAA AAGCCAAAGA AGGAAGAGAC GCCGGCAGCA 
CCGGAGAAAA AAGACGCCAA GGAGGGGAAG GCCGCAGAAG CCAAGAAGCC TGAGGAGAAA 
CCCAAAACAG AGGCCCAAGC CAAGGAACCT GGCAAGGAGG CCTCCACACC CGGGAAAGCC 
AAGGCTGAGA AATCCTCCAG CACAGACCAG AAAGACAGCA GACCTGCAGA GAAGGCCGCA 
GAAGATAAGG CCACCAAGGG AGAGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEFH ( XM_005218114.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005218171.1 Bos taurus neurofilament heavy (NEFH), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa28070
Clone ID Related Accession (Same CDS sequence)	XM_010823474.2 , XM_005218114.3 , XM_005218114.2
Accession Version	XM_005218114.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	neurofilament heavy polypeptide isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005218114.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCA GCGGCGCCGA CGCGCTGCTG GGCGCCTTCG CGCCGCTCCA CGGAGGCGGC 
AGCTTGCACT ATGCACTGGC TCGCAAAGGC GGCGCCGGCG GAGCGCGCTC TGCAGCCGGC 
TCCTCCAGCG GATTCCACTC ATGGGCGCGG ACCTCCGTGA GCTCCGTGTC GGCCTCCCCG 
AGCCGCTTCC GAGGCGCCGG GACTACCTCC AGCACCGACT CGCTAGACAC CCTGAGCAAC 
GGACCGGAGG GCTGCGTGGT GGTGGCGCGC AGTGAGAAGG AGCAGCTGCA GGTGCTGAAC 
GACCGCTTCG CGGGCTACAT CGACAAGGTG CGGCAGCTCG AGGCGCATAA CCGCAGCCTG 
GAGGGCGAGG CGGCGGCGCT GCGGCAGCAG CAGGCGGGCC GCGCAGCCAT GGGCGAGCTG 
TACGAGCGCG AGGTGCGCGA GATGCGCGGC GCAGTGCTGC GCTTGGGCGC GGCTCGCGGC 
CAGCTGCGCC TGGAGCAGGA GCACCTGCTG GAGGACATCG CGCACGTGCG CCAGCGCCTG 
GACGACGAGG CCCGGCAGCG CGAGGAGGCC GAGGCAGCGA CGCGCGCACT GGCCCGCTTT 
GCGCAGGAGG CCGAGGCGGC GCGCGTCGAG CTGCAGAAGA AGGCACAAGC CCTGCAGGAA 
GAGTGCGGCT ACCTGCGGCG TCACCACCAG GAGGAGGTGG GCGAGCTGCT CGGCCAGATC 
CAGAGCAGCA GCGCCGCGCA GACGCAGGCC GAGGCGCGCG ATGCCCTCAA GTGCGACGTG 
ACGTCGGCCC TGCGCGAGAT CCGCGCGCAG CTTGAAGGCC ACACGGTGCA GAGCACGCTT 
CAGTCCGAGG AGTGGTTCCG AGTGAGGCTG GACCGACTGT CCGAGGCAGC CAAGGTGAAC 
ACAGACGCCA TGCGCTCAGC ACAGGAGGAG ATATCTGAGT ACCGGCGCCA GCTGCAGGCC 
AGGACCACAG AGCTGGAGAC GCTCAAAAGC ACCAAGGACT CACTGGAGAG GCAGCGCTCT 
GAGCTGGAGG ACCGTCACCA GGCTGACATC GCATCCTACC AGGAGGCCAT CCAGCAGCTG 
GATGCAGAGC TGAGGAACAC CAAGTGGGAG ATGGCAGCCC AGCTCCGAGA GTACCAGGAT 
CTACTCAATG TCAAGATGGC TCTGGACATT GAGATCGCTG CTTACAGAAA ACTCCTGGAG 
GGTGAAGAAT GTCGAATTGG CTTTGGCCCG AGTCCTTTCC TCCTTCCAGA AGGACTCCCC 
AAGATCCCCT CCGTGTCCAC TCACATCAAA GTCAAAAGTG AAGAGAAGAT CAAGATGGTA 
GAAAAGTCAG AGAAGGAAAC TGTGATTGTG GAGGAACAGA CAGAGGAGGT CCAAGTGACT 
GAAGAAGTGA CTGAAGAAGA AGAGAAAGAG GCCAAAGAGG AGGAAGGAGA AGCAGTAAAG 
TCTCCCCCAG CAGAAGAGGC TGCATCCCCA GAGAAGGAGG AGGCCAAGTC ACCAGCCGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAGG AGGCCAAGTC CCCACAGAAG 
GAAGAGGCCA AGTCACCAGC TGAGGTGAAG TCCCCTGAGA AGGCCAAGTC CCCCGTGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAAG AAGCCAAATC CCCAGAGAAG 
GCCAAGTCCC CAGTGGAGGC CAAGTCTCCA GAAAAGGCCA AGTCCCCCAT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGT CAAATCCCCA GTGGAGGCCA AATCCCCAGA GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GAGAAAGCCA AGTCCCCTGT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GTGGAAGCCA AGTCCCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCA GTGGAGGCCA AGTCGCCAGA GAAGGCCAAG 
TCTCCTGTGA AGGAGGAGGC CAAGTCCCCA GAGAAGGCCA AGTCTCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT GTGAAAGAGG AGGCCAAGTC CCCAGAGAAG 
GCCAAGTCTC CTGTGAAGGA GGAGGCCAAG TCGCCAGAGA AGGCCAAGTC TCCTGTGAAA 
GAGGAGGCCA AGTCCCCAGA GAAGGCCAAG TCTCCTGTGA AAGAGGAGGC CAAGTCCCCA 
GAGAAGGCCA AGTCTCCTGC GAAGGAGGAG GCCAAGTCGC CAGAGAAGGC CAAGTCTCCT 
GTGAAAGAGG AGGCCAAGTC CCCAGAGAAG GCCAAGTCTC CTGTGAAAGA GGAGGCCAAG 
TCCCCAGAGA AGGCCAAGTC TCCTGCGAAG GAGGAGGCCA AGTCCCCAGA GAAGGCCAAG 
TCCCCAGAGA AGCCCAAAAG CCCTGTGAAG GAGGAGGCCA AGTCCCCAGA GAAGCCCAAA 
AGCCCTGTGA AGGAGGAGGC CAAGTCCCCA GAGAAGCCCA AAAGCCCTGT CAAGGAGGAG 
GCCAAATCCC CAGAGAAGGC CAAGTCTCCT GTGAAGGATG AGGCCAAGGC TCCTGGGAAG 
GAGGTCCCAA AGAAGGAGGA GGCCAAGTCC CCTGTGAAGG AGGAAGAAAA ACCCCAGGAG 
GTGAGAGCCA AAGAGCCCCC AAAGAAGGCA GAGGAAGAGA AAGCTCCAGC CATACCCAAA 
GCTGAGGAGA AGAAGGACAG CAAGAAAGAT GAGGTGCCCA AGAAGGAGGC TCCAAAGCCC 
GAGGAGAAGA AGGAGCCTGC TGTGGAAAAG CCCAAAGAAC CCAAAGCTGA AGCCAAGAAG 
GAAGAGGCTG AAGATAAGAA AAAAGCAACC ACCCCAGAGA AGGAGGCTCC TGCCAAGGGG 
AAGGAAGAGG CCAAGCCTAA AGAGAAGACT GAGGTGGCCA AGAAGGAACC AGATGATGCC 
AAGCCCAAAG AACCCAGCAA AGCAGCAGAA AAGCCAAAGA AGGAAGAGAC GCCGGCAGCA 
CCGGAGAAAA AAGACGCCAA GGAGGGGAAG GCCGCAGAAG CCAAGAAGCC TGAGGAGAAA 
CCCAAAACAG AGGCCCAAGC CAAGGAACCT GGCAAGGAGG CCTCCACACC CGGGAAAGCC 
AAGGCTGAGA AATCCTCCAG CACAGACCAG AAAGACAGCA GACCTGCAGA GAAGGCCGCA 
GAAGATAAGG CCACCAAGGG AGAGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005218171.1
CDS	76..3285
Translation	MSFSGADALLGAFAPLHGGGSLHYALARKGGAGGARSAAGSSSGFHSWARTSVSSVSASPSRFRGAGTTSSTDSLDTLSNGPEGCVVVARSEKEQLQVLNDRFAGYIDKVRQLEAHNRSLEGEAAALRQQQAGRAAMGELYEREVREMRGAVLRLGAARGQLRLEQEHLLEDIAHVRQRLDDEARQREEAEAATRALARFAQEAEAARVELQKKAQALQEECGYLRRHHQEEVGELLGQIQSSSAAQTQAEARDALKCDVTSALREIRAQLEGHTVQSTLQSEEWFRVRLDRLSEAAKVNTDAMRSAQEEISEYRRQLQARTTELETLKSTKDSLERQRSELEDRHQADIASYQEAIQQLDAELRNTKWEMAAQLREYQDLLNVKMALDIEIAAYRKLLEGEECRIGFGPSPFLLPEGLPKIPSVSTHIKVKSEEKIKMVEKSEKETVIVEEQTEEVQVTEEVTEEEEKEAKEEEGEAVKSPPAEEAASPEKEEAKSPAEAKSPEKAKSPVKEEAKSPQKEEAKSPAEVKSPEKAKSPVEAKSPEKAKSPVKEEAKSPEKAKSPVEAKSPEKAKSPMKEEAKSPEKVKSPVEAKSPEKEEAKSPEKAKSPEKAKSPVKEEAKSPEKAKSPVEAKSPVKEEAKSPEKAKSPVEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPAKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPAKEEAKSPEKAKSPEKPKSPVKEEAKSPEKPKSPVKEEAKSPEKPKSPVKEEAKSPEKAKSPVKDEAKAPGKEVPKKEEAKSPVKEEEKPQEVRAKEPPKKAEEEKAPAIPKAEEKKDSKKDEVPKKEAPKPEEKKEPAVEKPKEPKAEAKKEEAEDKKKATTPEKEAPAKGKEEAKPKEKTEVAKKEPDDAKPKEPSKAAEKPKKEETPAAPEKKDAKEGKAAEAKKPEEKPKTEAQAKEPGKEASTPGKAKAEKSSSTDQKDSRPAEKAAEDKATKGEK

Target ORF information:

RefSeq Version	XM_005218114.3
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofilament heavy (NEFH), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005218114.3

ORF Insert Sequence:

ATGAGCTTCA GCGGCGCCGA CGCGCTGCTG GGCGCCTTCG CGCCGCTCCA CGGAGGCGGC 
AGCTTGCACT ATGCACTGGC TCGCAAAGGC GGCGCCGGCG GAGCGCGCTC TGCAGCCGGC 
TCCTCCAGCG GATTCCACTC ATGGGCGCGG ACCTCCGTGA GCTCCGTGTC GGCCTCCCCG 
AGCCGCTTCC GAGGCGCCGG GACTACCTCC AGCACCGACT CGCTAGACAC CCTGAGCAAC 
GGACCGGAGG GCTGCGTGGT GGTGGCGCGC AGTGAGAAGG AGCAGCTGCA GGTGCTGAAC 
GACCGCTTCG CGGGCTACAT CGACAAGGTG CGGCAGCTCG AGGCGCATAA CCGCAGCCTG 
GAGGGCGAGG CGGCGGCGCT GCGGCAGCAG CAGGCGGGCC GCGCAGCCAT GGGCGAGCTG 
TACGAGCGCG AGGTGCGCGA GATGCGCGGC GCAGTGCTGC GCTTGGGCGC GGCTCGCGGC 
CAGCTGCGCC TGGAGCAGGA GCACCTGCTG GAGGACATCG CGCACGTGCG CCAGCGCCTG 
GACGACGAGG CCCGGCAGCG CGAGGAGGCC GAGGCAGCGA CGCGCGCACT GGCCCGCTTT 
GCGCAGGAGG CCGAGGCGGC GCGCGTCGAG CTGCAGAAGA AGGCACAAGC CCTGCAGGAA 
GAGTGCGGCT ACCTGCGGCG TCACCACCAG GAGGAGGTGG GCGAGCTGCT CGGCCAGATC 
CAGAGCAGCA GCGCCGCGCA GACGCAGGCC GAGGCGCGCG ATGCCCTCAA GTGCGACGTG 
ACGTCGGCCC TGCGCGAGAT CCGCGCGCAG CTTGAAGGCC ACACGGTGCA GAGCACGCTT 
CAGTCCGAGG AGTGGTTCCG AGTGAGGCTG GACCGACTGT CCGAGGCAGC CAAGGTGAAC 
ACAGACGCCA TGCGCTCAGC ACAGGAGGAG ATATCTGAGT ACCGGCGCCA GCTGCAGGCC 
AGGACCACAG AGCTGGAGAC GCTCAAAAGC ACCAAGGACT CACTGGAGAG GCAGCGCTCT 
GAGCTGGAGG ACCGTCACCA GGCTGACATC GCATCCTACC AGGAGGCCAT CCAGCAGCTG 
GATGCAGAGC TGAGGAACAC CAAGTGGGAG ATGGCAGCCC AGCTCCGAGA GTACCAGGAT 
CTACTCAATG TCAAGATGGC TCTGGACATT GAGATCGCTG CTTACAGAAA ACTCCTGGAG 
GGTGAAGAAT GTCGAATTGG CTTTGGCCCG AGTCCTTTCC TCCTTCCAGA AGGACTCCCC 
AAGATCCCCT CCGTGTCCAC TCACATCAAA GTCAAAAGTG AAGAGAAGAT CAAGATGGTA 
GAAAAGTCAG AGAAGGAAAC TGTGATTGTG GAGGAACAGA CAGAGGAGGT CCAAGTGACT 
GAAGAAGTGA CTGAAGAAGA AGAGAAAGAG GCCAAAGAGG AGGAAGGAGA AGCAGTAAAG 
TCTCCCCCAG CAGAAGAGGC TGCATCCCCA GAGAAGGAGG AGGCCAAGTC ACCAGCCGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAGG AGGCCAAGTC CCCACAGAAG 
GAAGAGGCCA AGTCACCAGC TGAGGTGAAG TCCCCTGAGA AGGCCAAGTC CCCCGTGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAAG AAGCCAAATC CCCAGAGAAG 
GCCAAGTCCC CAGTGGAGGC CAAGTCTCCA GAAAAGGCCA AGTCCCCCAT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGT CAAATCCCCA GTGGAGGCCA AATCCCCAGA GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GAGAAAGCCA AGTCCCCTGT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GTGGAAGCCA AGTCCCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCA GTGGAGGCCA AGTCGCCAGA GAAGGCCAAG 
TCTCCTGTGA AGGAGGAGGC CAAGTCCCCA GAGAAGGCCA AGTCTCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT GTGAAAGAGG AGGCCAAGTC CCCAGAGAAG 
GCCAAGTCTC CTGTGAAGGA GGAGGCCAAG TCGCCAGAGA AGGCCAAGTC TCCTGTGAAA 
GAGGAGGCCA AGTCCCCAGA GAAGGCCAAG TCTCCTGTGA AAGAGGAGGC CAAGTCCCCA 
GAGAAGGCCA AGTCTCCTGC GAAGGAGGAG GCCAAGTCGC CAGAGAAGGC CAAGTCTCCT 
GTGAAAGAGG AGGCCAAGTC CCCAGAGAAG GCCAAGTCTC CTGTGAAAGA GGAGGCCAAG 
TCCCCAGAGA AGGCCAAGTC TCCTGCGAAG GAGGAGGCCA AGTCCCCAGA GAAGGCCAAG 
TCCCCAGAGA AGCCCAAAAG CCCTGTGAAG GAGGAGGCCA AGTCCCCAGA GAAGCCCAAA 
AGCCCTGTGA AGGAGGAGGC CAAGTCCCCA GAGAAGCCCA AAAGCCCTGT CAAGGAGGAG 
GCCAAATCCC CAGAGAAGGC CAAGTCTCCT GTGAAGGATG AGGCCAAGGC TCCTGGGAAG 
GAGGTCCCAA AGAAGGAGGA GGCCAAGTCC CCTGTGAAGG AGGAAGAAAA ACCCCAGGAG 
GTGAGAGCCA AAGAGCCCCC AAAGAAGGCA GAGGAAGAGA AAGCTCCAGC CATACCCAAA 
GCTGAGGAGA AGAAGGACAG CAAGAAAGAT GAGGTGCCCA AGAAGGAGGC TCCAAAGCCC 
GAGGAGAAGA AGGAGCCTGC TGTGGAAAAG CCCAAAGAAC CCAAAGCTGA AGCCAAGAAG 
GAAGAGGCTG AAGATAAGAA AAAAGCAACC ACCCCAGAGA AGGAGGCTCC TGCCAAGGGG 
AAGGAAGAGG CCAAGCCTAA AGAGAAGACT GAGGTGGCCA AGAAGGAACC AGATGATGCC 
AAGCCCAAAG AACCCAGCAA AGCAGCAGAA AAGCCAAAGA AGGAAGAGAC GCCGGCAGCA 
CCGGAGAAAA AAGACGCCAA GGAGGGGAAG GCCGCAGAAG CCAAGAAGCC TGAGGAGAAA 
CCCAAAACAG AGGCCCAAGC CAAGGAACCT GGCAAGGAGG CCTCCACACC CGGGAAAGCC 
AAGGCTGAGA AATCCTCCAG CACAGACCAG AAAGACAGCA GACCTGCAGA GAAGGCCGCA 
GAAGATAAGG CCACCAAGGG AGAGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEFH ( XM_005218114.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005218171.1 Bos taurus neurofilament, heavy polypeptide (NEFH), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa28070
Clone ID Related Accession (Same CDS sequence)	XM_010823474.2 , XM_005218114.3 , XM_005218114.2
Accession Version	XM_005218114.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neurofilament heavy polypeptide isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104458.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005218114.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCA GCGGCGCCGA CGCGCTGCTG GGCGCCTTCG CGCCGCTCCA CGGAGGCGGC 
AGCTTGCACT ATGCACTGGC TCGCAAAGGC GGCGCCGGCG GAGCGCGCTC TGCAGCCGGC 
TCCTCCAGCG GATTCCACTC ATGGGCGCGG ACCTCCGTGA GCTCCGTGTC GGCCTCCCCG 
AGCCGCTTCC GAGGCGCCGG GACTACCTCC AGCACCGACT CGCTAGACAC CCTGAGCAAC 
GGACCGGAGG GCTGCGTGGT GGTGGCGCGC AGTGAGAAGG AGCAGCTGCA GGTGCTGAAC 
GACCGCTTCG CGGGCTACAT CGACAAGGTG CGGCAGCTCG AGGCGCATAA CCGCAGCCTG 
GAGGGCGAGG CGGCGGCGCT GCGGCAGCAG CAGGCGGGCC GCGCAGCCAT GGGCGAGCTG 
TACGAGCGCG AGGTGCGCGA GATGCGCGGC GCAGTGCTGC GCTTGGGCGC GGCTCGCGGC 
CAGCTGCGCC TGGAGCAGGA GCACCTGCTG GAGGACATCG CGCACGTGCG CCAGCGCCTG 
GACGACGAGG CCCGGCAGCG CGAGGAGGCC GAGGCAGCGA CGCGCGCACT GGCCCGCTTT 
GCGCAGGAGG CCGAGGCGGC GCGCGTCGAG CTGCAGAAGA AGGCACAAGC CCTGCAGGAA 
GAGTGCGGCT ACCTGCGGCG TCACCACCAG GAGGAGGTGG GCGAGCTGCT CGGCCAGATC 
CAGAGCAGCA GCGCCGCGCA GACGCAGGCC GAGGCGCGCG ATGCCCTCAA GTGCGACGTG 
ACGTCGGCCC TGCGCGAGAT CCGCGCGCAG CTTGAAGGCC ACACGGTGCA GAGCACGCTT 
CAGTCCGAGG AGTGGTTCCG AGTGAGGCTG GACCGACTGT CCGAGGCAGC CAAGGTGAAC 
ACAGACGCCA TGCGCTCAGC ACAGGAGGAG ATATCTGAGT ACCGGCGCCA GCTGCAGGCC 
AGGACCACAG AGCTGGAGAC GCTCAAAAGC ACCAAGGACT CACTGGAGAG GCAGCGCTCT 
GAGCTGGAGG ACCGTCACCA GGCTGACATC GCATCCTACC AGGAGGCCAT CCAGCAGCTG 
GATGCAGAGC TGAGGAACAC CAAGTGGGAG ATGGCAGCCC AGCTCCGAGA GTACCAGGAT 
CTACTCAATG TCAAGATGGC TCTGGACATT GAGATCGCTG CTTACAGAAA ACTCCTGGAG 
GGTGAAGAAT GTCGAATTGG CTTTGGCCCG AGTCCTTTCC TCCTTCCAGA AGGACTCCCC 
AAGATCCCCT CCGTGTCCAC TCACATCAAA GTCAAAAGTG AAGAGAAGAT CAAGATGGTA 
GAAAAGTCAG AGAAGGAAAC TGTGATTGTG GAGGAACAGA CAGAGGAGGT CCAAGTGACT 
GAAGAAGTGA CTGAAGAAGA AGAGAAAGAG GCCAAAGAGG AGGAAGGAGA AGCAGTAAAG 
TCTCCCCCAG CAGAAGAGGC TGCATCCCCA GAGAAGGAGG AGGCCAAGTC ACCAGCCGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAGG AGGCCAAGTC CCCACAGAAG 
GAAGAGGCCA AGTCACCAGC TGAGGTGAAG TCCCCTGAGA AGGCCAAGTC CCCCGTGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAAG AAGCCAAATC CCCAGAGAAG 
GCCAAGTCCC CAGTGGAGGC CAAGTCTCCA GAAAAGGCCA AGTCCCCCAT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGT CAAATCCCCA GTGGAGGCCA AATCCCCAGA GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GAGAAAGCCA AGTCCCCTGT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GTGGAAGCCA AGTCCCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCA GTGGAGGCCA AGTCGCCAGA GAAGGCCAAG 
TCTCCTGTGA AGGAGGAGGC CAAGTCCCCA GAGAAGGCCA AGTCTCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT GTGAAAGAGG AGGCCAAGTC CCCAGAGAAG 
GCCAAGTCTC CTGTGAAGGA GGAGGCCAAG TCGCCAGAGA AGGCCAAGTC TCCTGTGAAA 
GAGGAGGCCA AGTCCCCAGA GAAGGCCAAG TCTCCTGTGA AAGAGGAGGC CAAGTCCCCA 
GAGAAGGCCA AGTCTCCTGC GAAGGAGGAG GCCAAGTCGC CAGAGAAGGC CAAGTCTCCT 
GTGAAAGAGG AGGCCAAGTC CCCAGAGAAG GCCAAGTCTC CTGTGAAAGA GGAGGCCAAG 
TCCCCAGAGA AGGCCAAGTC TCCTGCGAAG GAGGAGGCCA AGTCCCCAGA GAAGGCCAAG 
TCCCCAGAGA AGCCCAAAAG CCCTGTGAAG GAGGAGGCCA AGTCCCCAGA GAAGCCCAAA 
AGCCCTGTGA AGGAGGAGGC CAAGTCCCCA GAGAAGCCCA AAAGCCCTGT CAAGGAGGAG 
GCCAAATCCC CAGAGAAGGC CAAGTCTCCT GTGAAGGATG AGGCCAAGGC TCCTGGGAAG 
GAGGTCCCAA AGAAGGAGGA GGCCAAGTCC CCTGTGAAGG AGGAAGAAAA ACCCCAGGAG 
GTGAGAGCCA AAGAGCCCCC AAAGAAGGCA GAGGAAGAGA AAGCTCCAGC CATACCCAAA 
GCTGAGGAGA AGAAGGACAG CAAGAAAGAT GAGGTGCCCA AGAAGGAGGC TCCAAAGCCC 
GAGGAGAAGA AGGAGCCTGC TGTGGAAAAG CCCAAAGAAC CCAAAGCTGA AGCCAAGAAG 
GAAGAGGCTG AAGATAAGAA AAAAGCAACC ACCCCAGAGA AGGAGGCTCC TGCCAAGGGG 
AAGGAAGAGG CCAAGCCTAA AGAGAAGACT GAGGTGGCCA AGAAGGAACC AGATGATGCC 
AAGCCCAAAG AACCCAGCAA AGCAGCAGAA AAGCCAAAGA AGGAAGAGAC GCCGGCAGCA 
CCGGAGAAAA AAGACGCCAA GGAGGGGAAG GCCGCAGAAG CCAAGAAGCC TGAGGAGAAA 
CCCAAAACAG AGGCCCAAGC CAAGGAACCT GGCAAGGAGG CCTCCACACC CGGGAAAGCC 
AAGGCTGAGA AATCCTCCAG CACAGACCAG AAAGACAGCA GACCTGCAGA GAAGGCCGCA 
GAAGATAAGG CCACCAAGGG AGAGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005218171.1
CDS	79..3288
Translation	MSFSGADALLGAFAPLHGGGSLHYALARKGGAGGARSAAGSSSGFHSWARTSVSSVSASPSRFRGAGTTSSTDSLDTLSNGPEGCVVVARSEKEQLQVLNDRFAGYIDKVRQLEAHNRSLEGEAAALRQQQAGRAAMGELYEREVREMRGAVLRLGAARGQLRLEQEHLLEDIAHVRQRLDDEARQREEAEAATRALARFAQEAEAARVELQKKAQALQEECGYLRRHHQEEVGELLGQIQSSSAAQTQAEARDALKCDVTSALREIRAQLEGHTVQSTLQSEEWFRVRLDRLSEAAKVNTDAMRSAQEEISEYRRQLQARTTELETLKSTKDSLERQRSELEDRHQADIASYQEAIQQLDAELRNTKWEMAAQLREYQDLLNVKMALDIEIAAYRKLLEGEECRIGFGPSPFLLPEGLPKIPSVSTHIKVKSEEKIKMVEKSEKETVIVEEQTEEVQVTEEVTEEEEKEAKEEEGEAVKSPPAEEAASPEKEEAKSPAEAKSPEKAKSPVKEEAKSPQKEEAKSPAEVKSPEKAKSPVEAKSPEKAKSPVKEEAKSPEKAKSPVEAKSPEKAKSPMKEEAKSPEKVKSPVEAKSPEKEEAKSPEKAKSPEKAKSPVKEEAKSPEKAKSPVEAKSPVKEEAKSPEKAKSPVEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPAKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPAKEEAKSPEKAKSPEKPKSPVKEEAKSPEKPKSPVKEEAKSPEKPKSPVKEEAKSPEKAKSPVKDEAKAPGKEVPKKEEAKSPVKEEEKPQEVRAKEPPKKAEEEKAPAIPKAEEKKDSKKDEVPKKEAPKPEEKKEPAVEKPKEPKAEAKKEEAEDKKKATTPEKEAPAKGKEEAKPKEKTEVAKKEPDDAKPKEPSKAAEKPKKEETPAAPEKKDAKEGKAAEAKKPEEKPKTEAQAKEPGKEASTPGKAKAEKSSSTDQKDSRPAEKAAEDKATKGEK

Target ORF information:

RefSeq Version	XM_005218114.2
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofilament, heavy polypeptide (NEFH), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005218114.2

ORF Insert Sequence:

ATGAGCTTCA GCGGCGCCGA CGCGCTGCTG GGCGCCTTCG CGCCGCTCCA CGGAGGCGGC 
AGCTTGCACT ATGCACTGGC TCGCAAAGGC GGCGCCGGCG GAGCGCGCTC TGCAGCCGGC 
TCCTCCAGCG GATTCCACTC ATGGGCGCGG ACCTCCGTGA GCTCCGTGTC GGCCTCCCCG 
AGCCGCTTCC GAGGCGCCGG GACTACCTCC AGCACCGACT CGCTAGACAC CCTGAGCAAC 
GGACCGGAGG GCTGCGTGGT GGTGGCGCGC AGTGAGAAGG AGCAGCTGCA GGTGCTGAAC 
GACCGCTTCG CGGGCTACAT CGACAAGGTG CGGCAGCTCG AGGCGCATAA CCGCAGCCTG 
GAGGGCGAGG CGGCGGCGCT GCGGCAGCAG CAGGCGGGCC GCGCAGCCAT GGGCGAGCTG 
TACGAGCGCG AGGTGCGCGA GATGCGCGGC GCAGTGCTGC GCTTGGGCGC GGCTCGCGGC 
CAGCTGCGCC TGGAGCAGGA GCACCTGCTG GAGGACATCG CGCACGTGCG CCAGCGCCTG 
GACGACGAGG CCCGGCAGCG CGAGGAGGCC GAGGCAGCGA CGCGCGCACT GGCCCGCTTT 
GCGCAGGAGG CCGAGGCGGC GCGCGTCGAG CTGCAGAAGA AGGCACAAGC CCTGCAGGAA 
GAGTGCGGCT ACCTGCGGCG TCACCACCAG GAGGAGGTGG GCGAGCTGCT CGGCCAGATC 
CAGAGCAGCA GCGCCGCGCA GACGCAGGCC GAGGCGCGCG ATGCCCTCAA GTGCGACGTG 
ACGTCGGCCC TGCGCGAGAT CCGCGCGCAG CTTGAAGGCC ACACGGTGCA GAGCACGCTT 
CAGTCCGAGG AGTGGTTCCG AGTGAGGCTG GACCGACTGT CCGAGGCAGC CAAGGTGAAC 
ACAGACGCCA TGCGCTCAGC ACAGGAGGAG ATATCTGAGT ACCGGCGCCA GCTGCAGGCC 
AGGACCACAG AGCTGGAGAC GCTCAAAAGC ACCAAGGACT CACTGGAGAG GCAGCGCTCT 
GAGCTGGAGG ACCGTCACCA GGCTGACATC GCATCCTACC AGGAGGCCAT CCAGCAGCTG 
GATGCAGAGC TGAGGAACAC CAAGTGGGAG ATGGCAGCCC AGCTCCGAGA GTACCAGGAT 
CTACTCAATG TCAAGATGGC TCTGGACATT GAGATCGCTG CTTACAGAAA ACTCCTGGAG 
GGTGAAGAAT GTCGAATTGG CTTTGGCCCG AGTCCTTTCC TCCTTCCAGA AGGACTCCCC 
AAGATCCCCT CCGTGTCCAC TCACATCAAA GTCAAAAGTG AAGAGAAGAT CAAGATGGTA 
GAAAAGTCAG AGAAGGAAAC TGTGATTGTG GAGGAACAGA CAGAGGAGGT CCAAGTGACT 
GAAGAAGTGA CTGAAGAAGA AGAGAAAGAG GCCAAAGAGG AGGAAGGAGA AGCAGTAAAG 
TCTCCCCCAG CAGAAGAGGC TGCATCCCCA GAGAAGGAGG AGGCCAAGTC ACCAGCCGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAGG AGGCCAAGTC CCCACAGAAG 
GAAGAGGCCA AGTCACCAGC TGAGGTGAAG TCCCCTGAGA AGGCCAAGTC CCCCGTGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAAG AAGCCAAATC CCCAGAGAAG 
GCCAAGTCCC CAGTGGAGGC CAAGTCTCCA GAAAAGGCCA AGTCCCCCAT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGT CAAATCCCCA GTGGAGGCCA AATCCCCAGA GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GAGAAAGCCA AGTCCCCTGT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GTGGAAGCCA AGTCCCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCA GTGGAGGCCA AGTCGCCAGA GAAGGCCAAG 
TCTCCTGTGA AGGAGGAGGC CAAGTCCCCA GAGAAGGCCA AGTCTCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT GTGAAAGAGG AGGCCAAGTC CCCAGAGAAG 
GCCAAGTCTC CTGTGAAGGA GGAGGCCAAG TCGCCAGAGA AGGCCAAGTC TCCTGTGAAA 
GAGGAGGCCA AGTCCCCAGA GAAGGCCAAG TCTCCTGTGA AAGAGGAGGC CAAGTCCCCA 
GAGAAGGCCA AGTCTCCTGC GAAGGAGGAG GCCAAGTCGC CAGAGAAGGC CAAGTCTCCT 
GTGAAAGAGG AGGCCAAGTC CCCAGAGAAG GCCAAGTCTC CTGTGAAAGA GGAGGCCAAG 
TCCCCAGAGA AGGCCAAGTC TCCTGCGAAG GAGGAGGCCA AGTCCCCAGA GAAGGCCAAG 
TCCCCAGAGA AGCCCAAAAG CCCTGTGAAG GAGGAGGCCA AGTCCCCAGA GAAGCCCAAA 
AGCCCTGTGA AGGAGGAGGC CAAGTCCCCA GAGAAGCCCA AAAGCCCTGT CAAGGAGGAG 
GCCAAATCCC CAGAGAAGGC CAAGTCTCCT GTGAAGGATG AGGCCAAGGC TCCTGGGAAG 
GAGGTCCCAA AGAAGGAGGA GGCCAAGTCC CCTGTGAAGG AGGAAGAAAA ACCCCAGGAG 
GTGAGAGCCA AAGAGCCCCC AAAGAAGGCA GAGGAAGAGA AAGCTCCAGC CATACCCAAA 
GCTGAGGAGA AGAAGGACAG CAAGAAAGAT GAGGTGCCCA AGAAGGAGGC TCCAAAGCCC 
GAGGAGAAGA AGGAGCCTGC TGTGGAAAAG CCCAAAGAAC CCAAAGCTGA AGCCAAGAAG 
GAAGAGGCTG AAGATAAGAA AAAAGCAACC ACCCCAGAGA AGGAGGCTCC TGCCAAGGGG 
AAGGAAGAGG CCAAGCCTAA AGAGAAGACT GAGGTGGCCA AGAAGGAACC AGATGATGCC 
AAGCCCAAAG AACCCAGCAA AGCAGCAGAA AAGCCAAAGA AGGAAGAGAC GCCGGCAGCA 
CCGGAGAAAA AAGACGCCAA GGAGGGGAAG GCCGCAGAAG CCAAGAAGCC TGAGGAGAAA 
CCCAAAACAG AGGCCCAAGC CAAGGAACCT GGCAAGGAGG CCTCCACACC CGGGAAAGCC 
AAGGCTGAGA AATCCTCCAG CACAGACCAG AAAGACAGCA GACCTGCAGA GAAGGCCGCA 
GAAGATAAGG CCACCAAGGG AGAGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEFH ( XM_010823475.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010821777.2 Bos taurus neurofilament, heavy polypeptide (NEFH), transcript variant X3, mRNA.

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CloneID	OBa28071
Clone ID Related Accession (Same CDS sequence)	XM_010823475.2 , XM_005218115.3 , XM_005218115.2
Accession Version	XM_010823475.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neurofilament heavy polypeptide isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646373.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010823475.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCA GCGGCGCCGA CGCGCTGCTG GGCGCCTTCG CGCCGCTCCA CGGAGGCGGC 
AGCTTGCACT ATGCACTGGC TCGCAAAGGC GGCGCCGGCG GAGCGCGCTC TGCAGCCGGC 
TCCTCCAGCG GATTCCACTC ATGGGCGCGG ACCTCCGTGA GCTCCGTGTC GGCCTCCCCG 
AGCCGCTTCC GAGGCGCCGG GACTACCTCC AGCACCGACT CGCTAGACAC CCTGAGCAAC 
GGACCGGAGG GCTGCGTGGT GGTGGCGCGC AGTGAGAAGG AGCAGCTGCA GGTGCTGAAC 
GACCGCTTCG CGGGCTACAT CGACAAGGTG CGGCAGCTCG AGGCGCATAA CCGCAGCCTG 
GAGGGCGAGG CGGCGGCGCT GCGGCAGCAG CAGGCGGGCC GCGCAGCCAT GGGCGAGCTG 
TACGAGCGCG AGGTGCGCGA GATGCGCGGC GCAGTGCTGC GCTTGGGCGC GGCTCGCGGC 
CAGCTGCGCC TGGAGCAGGA GCACCTGCTG GAGGACATCG CGCACGTGCG CCAGCGCCTG 
GACGACGAGG CCCGGCAGCG CGAGGAGGCC GAGGCAGCGA CGCGCGCACT GGCCCGCTTT 
GCGCAGGAGG CCGAGGCGGC GCGCGTCGAG CTGCAGAAGA AGGCACAAGC CCTGCAGGAA 
GAGTGCGGCT ACCTGCGGCG TCACCACCAG GAGGAGGTGG GCGAGCTGCT CGGCCAGATC 
CAGAGCAGCA GCGCCGCGCA GACGCAGGCC GAGGCGCGCG ATGCCCTCAA GTGCGACGTG 
ACGTCGGCCC TGCGCGAGAT CCGCGCGCAG CTTGAAGGCC ACACGGTGCA GAGCACGCTT 
CAGTCCGAGG AGTGGTTCCG AGTGAGGCTG GACCGACTGT CCGAGGCAGC CAAGGTGAAC 
ACAGACGCCA TGCGCTCAGC ACAGGAGGAG ATATCTGAGT ACCGGCGCCA GCTGCAGGCC 
AGGACCACAG AGCTGGAGAC GCTCAAAAGC ACCAAGGACT CACTGGAGAG GCAGCGCTCT 
GAGCTGGAGG ACCGTCACCA GGCTGACATC GCATCCTACC AGGAGGCCAT CCAGCAGCTG 
GATGCAGAGC TGAGGAACAC CAAGTGGGAG ATGGCAGCCC AGCTCCGAGA GTACCAGGAT 
CTACTCAATG TCAAGATGGC TCTGGACATT GAGATCGCTG CTTACAGAAA ACTCCTGGAG 
GGTGAAGAAT GTCGAATTGG CTTTGGCCCG AGTCCTTTCC TCCTTCCAGA AGGACTCCCC 
AAGATCCCCT CCGTGTCCAC TCACATCAAA GTCAAAAGTG AAGAGAAGAT CAAGATGGTA 
GAAAAGTCAG AGAAGGAAAC TGTGATTGTG GAGGAACAGA CAGAGGAGGT CCAAGTGACT 
GAAGAAGTGA CTGAAGAAGA AGAGAAAGAG GCCAAAGAGG AGGAAGGAGA AGCAGTAAAG 
TCTCCCCCAG CAGAAGAGGC TGCATCCCCA GAGAAGGAGG AGGCCAAGTC ACCAGCCGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAGG AGGCCAAGTC CCCACAGAAG 
GAAGAGGCCA AGTCACCAGC TGAGGTGAAG TCCCCTGAGA AGGCCAAGTC CCCCGTGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAAG AAGCCAAATC CCCAGAGAAG 
GCCAAGTCCC CAGTGGAGGC CAAGTCTCCA GAAAAGGCCA AGTCCCCCAT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGT CAAATCCCCA GTGGAGGCCA AATCCCCAGA GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GAGAAAGCCA AGTCCCCTGT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GTGGAAGCCA AGTCCCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCA GTGGAGGCCA AGTCGCCAGA GAAGGCCAAG 
TCTCCTGTGA AGGAGGAGGC CAAGTCCCCA GAGAAGGCCA AGTCTCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT GTGAAAGAGG AGGCCAAGTC CCCAGAGAAG 
GCCAAGTCTC CTGTGAAGGA GGAGGCCAAG TCGCCAGAGA AGGCCAAGTC TCCTGTGAAA 
GAGGAGGCCA AGTCCCCAGA GAAGGCCAAG TCTCCTGTGA AAGAGGAGGC CAAGTCCCCA 
GAGAAGGCCA AGTCCCCAGT AGAGGCCAAG TCCCCAGAGA AGGCCAAGTC TCCTGCGAAG 
GAGGAGGCCA AGTCGCCAGA GAAGGCCAAG TCTCCTGTGA AAGAGGAGGC CAAGTCCCCA 
GAGAAGGCCA AGTCTCCTGT GAAAGAGGAG GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT 
GCGAAGGAGG AGGCCAAGTC CCCAGAGAAG GCCAAGTCCC CAGAGAAGCC CAAAAGCCCT 
GTGAAGGAGG AGGCCAAGTC CCCAGAGAAG CCCAAAAGCC CTGTCAAGGA GGAGGCCAAA 
TCCCCAGAGA AGGCCAAGTC TCCTGTGAAG GATGAGGCCA AGGCTCCTGG GAAGGAGGTC 
CCAAAGAAGG AGGAGGCCAA GTCCCCTGTG AAGGAGGAAG AAAAACCCCA GGAGGTGAGA 
GCCAAAGAGC CCCCAAAGAA GGCAGAGGAA GAGAAAGCTC CAGCCATACC CAAAGCTGAG 
GAGAAGAAGG ACAGCAAGAA AGATGAGGTG CCCAAGAAGG AGGCTCCAAA GCCCGAGGAG 
AAGAAGGAGC CTGCTGTGGA AAAGCCCAAA GAACCCAAAG CTGAAGCCAA GAAGGAAGAG 
GCTGAAGATA AGAAAAAAGC AACCACCCCA GAGAAGGAGG CTCCTGCCAA GGGGAAGGAA 
GAGGCCAAGC CTAAAGAGAA GACTGAGGTG GCCAAGAAGG AACCAGATGA TGCCAAGCCC 
AAAGAACCCA GCAAAGCAGC AGAAAAGCCA AAGAAGGAAG AGACGCCGGC AGCACCGGAG 
AAAAAAGACG CCAAGGAGGG GAAGGCCGCA GAAGCCAAGA AGCCTGAGGA GAAACCCAAA 
ACAGAGGCCC AAGCCAAGGA ACCTGGCAAG GAGGCCTCCA CACCCGGGAA AGCCAAGGCT 
GAGAAATCCT CCAGCACAGA CCAGAAAGAC AGCAGACCTG CAGAGAAGGC CGCAGAAGAT 
AAGGCCACCA AGGGAGAGAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010821777.2
CDS	79..3282
Translation	MSFSGADALLGAFAPLHGGGSLHYALARKGGAGGARSAAGSSSGFHSWARTSVSSVSASPSRFRGAGTTSSTDSLDTLSNGPEGCVVVARSEKEQLQVLNDRFAGYIDKVRQLEAHNRSLEGEAAALRQQQAGRAAMGELYEREVREMRGAVLRLGAARGQLRLEQEHLLEDIAHVRQRLDDEARQREEAEAATRALARFAQEAEAARVELQKKAQALQEECGYLRRHHQEEVGELLGQIQSSSAAQTQAEARDALKCDVTSALREIRAQLEGHTVQSTLQSEEWFRVRLDRLSEAAKVNTDAMRSAQEEISEYRRQLQARTTELETLKSTKDSLERQRSELEDRHQADIASYQEAIQQLDAELRNTKWEMAAQLREYQDLLNVKMALDIEIAAYRKLLEGEECRIGFGPSPFLLPEGLPKIPSVSTHIKVKSEEKIKMVEKSEKETVIVEEQTEEVQVTEEVTEEEEKEAKEEEGEAVKSPPAEEAASPEKEEAKSPAEAKSPEKAKSPVKEEAKSPQKEEAKSPAEVKSPEKAKSPVEAKSPEKAKSPVKEEAKSPEKAKSPVEAKSPEKAKSPMKEEAKSPEKVKSPVEAKSPEKEEAKSPEKAKSPEKAKSPVKEEAKSPEKAKSPVEAKSPVKEEAKSPEKAKSPVEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVEAKSPEKAKSPAKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPAKEEAKSPEKAKSPEKPKSPVKEEAKSPEKPKSPVKEEAKSPEKAKSPVKDEAKAPGKEVPKKEEAKSPVKEEEKPQEVRAKEPPKKAEEEKAPAIPKAEEKKDSKKDEVPKKEAPKPEEKKEPAVEKPKEPKAEAKKEEAEDKKKATTPEKEAPAKGKEEAKPKEKTEVAKKEPDDAKPKEPSKAAEKPKKEETPAAPEKKDAKEGKAAEAKKPEEKPKTEAQAKEPGKEASTPGKAKAEKSSSTDQKDSRPAEKAAEDKATKGEK

Target ORF information:

RefSeq Version	XM_010823475.2
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofilament, heavy polypeptide (NEFH), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010823475.2

ORF Insert Sequence:

ATGAGCTTCA GCGGCGCCGA CGCGCTGCTG GGCGCCTTCG CGCCGCTCCA CGGAGGCGGC 
AGCTTGCACT ATGCACTGGC TCGCAAAGGC GGCGCCGGCG GAGCGCGCTC TGCAGCCGGC 
TCCTCCAGCG GATTCCACTC ATGGGCGCGG ACCTCCGTGA GCTCCGTGTC GGCCTCCCCG 
AGCCGCTTCC GAGGCGCCGG GACTACCTCC AGCACCGACT CGCTAGACAC CCTGAGCAAC 
GGACCGGAGG GCTGCGTGGT GGTGGCGCGC AGTGAGAAGG AGCAGCTGCA GGTGCTGAAC 
GACCGCTTCG CGGGCTACAT CGACAAGGTG CGGCAGCTCG AGGCGCATAA CCGCAGCCTG 
GAGGGCGAGG CGGCGGCGCT GCGGCAGCAG CAGGCGGGCC GCGCAGCCAT GGGCGAGCTG 
TACGAGCGCG AGGTGCGCGA GATGCGCGGC GCAGTGCTGC GCTTGGGCGC GGCTCGCGGC 
CAGCTGCGCC TGGAGCAGGA GCACCTGCTG GAGGACATCG CGCACGTGCG CCAGCGCCTG 
GACGACGAGG CCCGGCAGCG CGAGGAGGCC GAGGCAGCGA CGCGCGCACT GGCCCGCTTT 
GCGCAGGAGG CCGAGGCGGC GCGCGTCGAG CTGCAGAAGA AGGCACAAGC CCTGCAGGAA 
GAGTGCGGCT ACCTGCGGCG TCACCACCAG GAGGAGGTGG GCGAGCTGCT CGGCCAGATC 
CAGAGCAGCA GCGCCGCGCA GACGCAGGCC GAGGCGCGCG ATGCCCTCAA GTGCGACGTG 
ACGTCGGCCC TGCGCGAGAT CCGCGCGCAG CTTGAAGGCC ACACGGTGCA GAGCACGCTT 
CAGTCCGAGG AGTGGTTCCG AGTGAGGCTG GACCGACTGT CCGAGGCAGC CAAGGTGAAC 
ACAGACGCCA TGCGCTCAGC ACAGGAGGAG ATATCTGAGT ACCGGCGCCA GCTGCAGGCC 
AGGACCACAG AGCTGGAGAC GCTCAAAAGC ACCAAGGACT CACTGGAGAG GCAGCGCTCT 
GAGCTGGAGG ACCGTCACCA GGCTGACATC GCATCCTACC AGGAGGCCAT CCAGCAGCTG 
GATGCAGAGC TGAGGAACAC CAAGTGGGAG ATGGCAGCCC AGCTCCGAGA GTACCAGGAT 
CTACTCAATG TCAAGATGGC TCTGGACATT GAGATCGCTG CTTACAGAAA ACTCCTGGAG 
GGTGAAGAAT GTCGAATTGG CTTTGGCCCG AGTCCTTTCC TCCTTCCAGA AGGACTCCCC 
AAGATCCCCT CCGTGTCCAC TCACATCAAA GTCAAAAGTG AAGAGAAGAT CAAGATGGTA 
GAAAAGTCAG AGAAGGAAAC TGTGATTGTG GAGGAACAGA CAGAGGAGGT CCAAGTGACT 
GAAGAAGTGA CTGAAGAAGA AGAGAAAGAG GCCAAAGAGG AGGAAGGAGA AGCAGTAAAG 
TCTCCCCCAG CAGAAGAGGC TGCATCCCCA GAGAAGGAGG AGGCCAAGTC ACCAGCCGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAGG AGGCCAAGTC CCCACAGAAG 
GAAGAGGCCA AGTCACCAGC TGAGGTGAAG TCCCCTGAGA AGGCCAAGTC CCCCGTGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAAG AAGCCAAATC CCCAGAGAAG 
GCCAAGTCCC CAGTGGAGGC CAAGTCTCCA GAAAAGGCCA AGTCCCCCAT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGT CAAATCCCCA GTGGAGGCCA AATCCCCAGA GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GAGAAAGCCA AGTCCCCTGT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GTGGAAGCCA AGTCCCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCA GTGGAGGCCA AGTCGCCAGA GAAGGCCAAG 
TCTCCTGTGA AGGAGGAGGC CAAGTCCCCA GAGAAGGCCA AGTCTCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT GTGAAAGAGG AGGCCAAGTC CCCAGAGAAG 
GCCAAGTCTC CTGTGAAGGA GGAGGCCAAG TCGCCAGAGA AGGCCAAGTC TCCTGTGAAA 
GAGGAGGCCA AGTCCCCAGA GAAGGCCAAG TCTCCTGTGA AAGAGGAGGC CAAGTCCCCA 
GAGAAGGCCA AGTCCCCAGT AGAGGCCAAG TCCCCAGAGA AGGCCAAGTC TCCTGCGAAG 
GAGGAGGCCA AGTCGCCAGA GAAGGCCAAG TCTCCTGTGA AAGAGGAGGC CAAGTCCCCA 
GAGAAGGCCA AGTCTCCTGT GAAAGAGGAG GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT 
GCGAAGGAGG AGGCCAAGTC CCCAGAGAAG GCCAAGTCCC CAGAGAAGCC CAAAAGCCCT 
GTGAAGGAGG AGGCCAAGTC CCCAGAGAAG CCCAAAAGCC CTGTCAAGGA GGAGGCCAAA 
TCCCCAGAGA AGGCCAAGTC TCCTGTGAAG GATGAGGCCA AGGCTCCTGG GAAGGAGGTC 
CCAAAGAAGG AGGAGGCCAA GTCCCCTGTG AAGGAGGAAG AAAAACCCCA GGAGGTGAGA 
GCCAAAGAGC CCCCAAAGAA GGCAGAGGAA GAGAAAGCTC CAGCCATACC CAAAGCTGAG 
GAGAAGAAGG ACAGCAAGAA AGATGAGGTG CCCAAGAAGG AGGCTCCAAA GCCCGAGGAG 
AAGAAGGAGC CTGCTGTGGA AAAGCCCAAA GAACCCAAAG CTGAAGCCAA GAAGGAAGAG 
GCTGAAGATA AGAAAAAAGC AACCACCCCA GAGAAGGAGG CTCCTGCCAA GGGGAAGGAA 
GAGGCCAAGC CTAAAGAGAA GACTGAGGTG GCCAAGAAGG AACCAGATGA TGCCAAGCCC 
AAAGAACCCA GCAAAGCAGC AGAAAAGCCA AAGAAGGAAG AGACGCCGGC AGCACCGGAG 
AAAAAAGACG CCAAGGAGGG GAAGGCCGCA GAAGCCAAGA AGCCTGAGGA GAAACCCAAA 
ACAGAGGCCC AAGCCAAGGA ACCTGGCAAG GAGGCCTCCA CACCCGGGAA AGCCAAGGCT 
GAGAAATCCT CCAGCACAGA CCAGAAAGAC AGCAGACCTG CAGAGAAGGC CGCAGAAGAT 
AAGGCCACCA AGGGAGAGAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEFH ( XM_005218115.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005218172.1 Bos taurus neurofilament heavy (NEFH), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa28071
Clone ID Related Accession (Same CDS sequence)	XM_010823475.2 , XM_005218115.3 , XM_005218115.2
Accession Version	XM_005218115.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	neurofilament heavy polypeptide isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005218115.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCA GCGGCGCCGA CGCGCTGCTG GGCGCCTTCG CGCCGCTCCA CGGAGGCGGC 
AGCTTGCACT ATGCACTGGC TCGCAAAGGC GGCGCCGGCG GAGCGCGCTC TGCAGCCGGC 
TCCTCCAGCG GATTCCACTC ATGGGCGCGG ACCTCCGTGA GCTCCGTGTC GGCCTCCCCG 
AGCCGCTTCC GAGGCGCCGG GACTACCTCC AGCACCGACT CGCTAGACAC CCTGAGCAAC 
GGACCGGAGG GCTGCGTGGT GGTGGCGCGC AGTGAGAAGG AGCAGCTGCA GGTGCTGAAC 
GACCGCTTCG CGGGCTACAT CGACAAGGTG CGGCAGCTCG AGGCGCATAA CCGCAGCCTG 
GAGGGCGAGG CGGCGGCGCT GCGGCAGCAG CAGGCGGGCC GCGCAGCCAT GGGCGAGCTG 
TACGAGCGCG AGGTGCGCGA GATGCGCGGC GCAGTGCTGC GCTTGGGCGC GGCTCGCGGC 
CAGCTGCGCC TGGAGCAGGA GCACCTGCTG GAGGACATCG CGCACGTGCG CCAGCGCCTG 
GACGACGAGG CCCGGCAGCG CGAGGAGGCC GAGGCAGCGA CGCGCGCACT GGCCCGCTTT 
GCGCAGGAGG CCGAGGCGGC GCGCGTCGAG CTGCAGAAGA AGGCACAAGC CCTGCAGGAA 
GAGTGCGGCT ACCTGCGGCG TCACCACCAG GAGGAGGTGG GCGAGCTGCT CGGCCAGATC 
CAGAGCAGCA GCGCCGCGCA GACGCAGGCC GAGGCGCGCG ATGCCCTCAA GTGCGACGTG 
ACGTCGGCCC TGCGCGAGAT CCGCGCGCAG CTTGAAGGCC ACACGGTGCA GAGCACGCTT 
CAGTCCGAGG AGTGGTTCCG AGTGAGGCTG GACCGACTGT CCGAGGCAGC CAAGGTGAAC 
ACAGACGCCA TGCGCTCAGC ACAGGAGGAG ATATCTGAGT ACCGGCGCCA GCTGCAGGCC 
AGGACCACAG AGCTGGAGAC GCTCAAAAGC ACCAAGGACT CACTGGAGAG GCAGCGCTCT 
GAGCTGGAGG ACCGTCACCA GGCTGACATC GCATCCTACC AGGAGGCCAT CCAGCAGCTG 
GATGCAGAGC TGAGGAACAC CAAGTGGGAG ATGGCAGCCC AGCTCCGAGA GTACCAGGAT 
CTACTCAATG TCAAGATGGC TCTGGACATT GAGATCGCTG CTTACAGAAA ACTCCTGGAG 
GGTGAAGAAT GTCGAATTGG CTTTGGCCCG AGTCCTTTCC TCCTTCCAGA AGGACTCCCC 
AAGATCCCCT CCGTGTCCAC TCACATCAAA GTCAAAAGTG AAGAGAAGAT CAAGATGGTA 
GAAAAGTCAG AGAAGGAAAC TGTGATTGTG GAGGAACAGA CAGAGGAGGT CCAAGTGACT 
GAAGAAGTGA CTGAAGAAGA AGAGAAAGAG GCCAAAGAGG AGGAAGGAGA AGCAGTAAAG 
TCTCCCCCAG CAGAAGAGGC TGCATCCCCA GAGAAGGAGG AGGCCAAGTC ACCAGCCGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAGG AGGCCAAGTC CCCACAGAAG 
GAAGAGGCCA AGTCACCAGC TGAGGTGAAG TCCCCTGAGA AGGCCAAGTC CCCCGTGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAAG AAGCCAAATC CCCAGAGAAG 
GCCAAGTCCC CAGTGGAGGC CAAGTCTCCA GAAAAGGCCA AGTCCCCCAT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGT CAAATCCCCA GTGGAGGCCA AATCCCCAGA GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GAGAAAGCCA AGTCCCCTGT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GTGGAAGCCA AGTCCCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCA GTGGAGGCCA AGTCGCCAGA GAAGGCCAAG 
TCTCCTGTGA AGGAGGAGGC CAAGTCCCCA GAGAAGGCCA AGTCTCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT GTGAAAGAGG AGGCCAAGTC CCCAGAGAAG 
GCCAAGTCTC CTGTGAAGGA GGAGGCCAAG TCGCCAGAGA AGGCCAAGTC TCCTGTGAAA 
GAGGAGGCCA AGTCCCCAGA GAAGGCCAAG TCTCCTGTGA AAGAGGAGGC CAAGTCCCCA 
GAGAAGGCCA AGTCCCCAGT AGAGGCCAAG TCCCCAGAGA AGGCCAAGTC TCCTGCGAAG 
GAGGAGGCCA AGTCGCCAGA GAAGGCCAAG TCTCCTGTGA AAGAGGAGGC CAAGTCCCCA 
GAGAAGGCCA AGTCTCCTGT GAAAGAGGAG GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT 
GCGAAGGAGG AGGCCAAGTC CCCAGAGAAG GCCAAGTCCC CAGAGAAGCC CAAAAGCCCT 
GTGAAGGAGG AGGCCAAGTC CCCAGAGAAG CCCAAAAGCC CTGTCAAGGA GGAGGCCAAA 
TCCCCAGAGA AGGCCAAGTC TCCTGTGAAG GATGAGGCCA AGGCTCCTGG GAAGGAGGTC 
CCAAAGAAGG AGGAGGCCAA GTCCCCTGTG AAGGAGGAAG AAAAACCCCA GGAGGTGAGA 
GCCAAAGAGC CCCCAAAGAA GGCAGAGGAA GAGAAAGCTC CAGCCATACC CAAAGCTGAG 
GAGAAGAAGG ACAGCAAGAA AGATGAGGTG CCCAAGAAGG AGGCTCCAAA GCCCGAGGAG 
AAGAAGGAGC CTGCTGTGGA AAAGCCCAAA GAACCCAAAG CTGAAGCCAA GAAGGAAGAG 
GCTGAAGATA AGAAAAAAGC AACCACCCCA GAGAAGGAGG CTCCTGCCAA GGGGAAGGAA 
GAGGCCAAGC CTAAAGAGAA GACTGAGGTG GCCAAGAAGG AACCAGATGA TGCCAAGCCC 
AAAGAACCCA GCAAAGCAGC AGAAAAGCCA AAGAAGGAAG AGACGCCGGC AGCACCGGAG 
AAAAAAGACG CCAAGGAGGG GAAGGCCGCA GAAGCCAAGA AGCCTGAGGA GAAACCCAAA 
ACAGAGGCCC AAGCCAAGGA ACCTGGCAAG GAGGCCTCCA CACCCGGGAA AGCCAAGGCT 
GAGAAATCCT CCAGCACAGA CCAGAAAGAC AGCAGACCTG CAGAGAAGGC CGCAGAAGAT 
AAGGCCACCA AGGGAGAGAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005218172.1
CDS	76..3279
Translation	MSFSGADALLGAFAPLHGGGSLHYALARKGGAGGARSAAGSSSGFHSWARTSVSSVSASPSRFRGAGTTSSTDSLDTLSNGPEGCVVVARSEKEQLQVLNDRFAGYIDKVRQLEAHNRSLEGEAAALRQQQAGRAAMGELYEREVREMRGAVLRLGAARGQLRLEQEHLLEDIAHVRQRLDDEARQREEAEAATRALARFAQEAEAARVELQKKAQALQEECGYLRRHHQEEVGELLGQIQSSSAAQTQAEARDALKCDVTSALREIRAQLEGHTVQSTLQSEEWFRVRLDRLSEAAKVNTDAMRSAQEEISEYRRQLQARTTELETLKSTKDSLERQRSELEDRHQADIASYQEAIQQLDAELRNTKWEMAAQLREYQDLLNVKMALDIEIAAYRKLLEGEECRIGFGPSPFLLPEGLPKIPSVSTHIKVKSEEKIKMVEKSEKETVIVEEQTEEVQVTEEVTEEEEKEAKEEEGEAVKSPPAEEAASPEKEEAKSPAEAKSPEKAKSPVKEEAKSPQKEEAKSPAEVKSPEKAKSPVEAKSPEKAKSPVKEEAKSPEKAKSPVEAKSPEKAKSPMKEEAKSPEKVKSPVEAKSPEKEEAKSPEKAKSPEKAKSPVKEEAKSPEKAKSPVEAKSPVKEEAKSPEKAKSPVEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVEAKSPEKAKSPAKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPAKEEAKSPEKAKSPEKPKSPVKEEAKSPEKPKSPVKEEAKSPEKAKSPVKDEAKAPGKEVPKKEEAKSPVKEEEKPQEVRAKEPPKKAEEEKAPAIPKAEEKKDSKKDEVPKKEAPKPEEKKEPAVEKPKEPKAEAKKEEAEDKKKATTPEKEAPAKGKEEAKPKEKTEVAKKEPDDAKPKEPSKAAEKPKKEETPAAPEKKDAKEGKAAEAKKPEEKPKTEAQAKEPGKEASTPGKAKAEKSSSTDQKDSRPAEKAAEDKATKGEK

Target ORF information:

RefSeq Version	XM_005218115.3
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofilament heavy (NEFH), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005218115.3

ORF Insert Sequence:

ATGAGCTTCA GCGGCGCCGA CGCGCTGCTG GGCGCCTTCG CGCCGCTCCA CGGAGGCGGC 
AGCTTGCACT ATGCACTGGC TCGCAAAGGC GGCGCCGGCG GAGCGCGCTC TGCAGCCGGC 
TCCTCCAGCG GATTCCACTC ATGGGCGCGG ACCTCCGTGA GCTCCGTGTC GGCCTCCCCG 
AGCCGCTTCC GAGGCGCCGG GACTACCTCC AGCACCGACT CGCTAGACAC CCTGAGCAAC 
GGACCGGAGG GCTGCGTGGT GGTGGCGCGC AGTGAGAAGG AGCAGCTGCA GGTGCTGAAC 
GACCGCTTCG CGGGCTACAT CGACAAGGTG CGGCAGCTCG AGGCGCATAA CCGCAGCCTG 
GAGGGCGAGG CGGCGGCGCT GCGGCAGCAG CAGGCGGGCC GCGCAGCCAT GGGCGAGCTG 
TACGAGCGCG AGGTGCGCGA GATGCGCGGC GCAGTGCTGC GCTTGGGCGC GGCTCGCGGC 
CAGCTGCGCC TGGAGCAGGA GCACCTGCTG GAGGACATCG CGCACGTGCG CCAGCGCCTG 
GACGACGAGG CCCGGCAGCG CGAGGAGGCC GAGGCAGCGA CGCGCGCACT GGCCCGCTTT 
GCGCAGGAGG CCGAGGCGGC GCGCGTCGAG CTGCAGAAGA AGGCACAAGC CCTGCAGGAA 
GAGTGCGGCT ACCTGCGGCG TCACCACCAG GAGGAGGTGG GCGAGCTGCT CGGCCAGATC 
CAGAGCAGCA GCGCCGCGCA GACGCAGGCC GAGGCGCGCG ATGCCCTCAA GTGCGACGTG 
ACGTCGGCCC TGCGCGAGAT CCGCGCGCAG CTTGAAGGCC ACACGGTGCA GAGCACGCTT 
CAGTCCGAGG AGTGGTTCCG AGTGAGGCTG GACCGACTGT CCGAGGCAGC CAAGGTGAAC 
ACAGACGCCA TGCGCTCAGC ACAGGAGGAG ATATCTGAGT ACCGGCGCCA GCTGCAGGCC 
AGGACCACAG AGCTGGAGAC GCTCAAAAGC ACCAAGGACT CACTGGAGAG GCAGCGCTCT 
GAGCTGGAGG ACCGTCACCA GGCTGACATC GCATCCTACC AGGAGGCCAT CCAGCAGCTG 
GATGCAGAGC TGAGGAACAC CAAGTGGGAG ATGGCAGCCC AGCTCCGAGA GTACCAGGAT 
CTACTCAATG TCAAGATGGC TCTGGACATT GAGATCGCTG CTTACAGAAA ACTCCTGGAG 
GGTGAAGAAT GTCGAATTGG CTTTGGCCCG AGTCCTTTCC TCCTTCCAGA AGGACTCCCC 
AAGATCCCCT CCGTGTCCAC TCACATCAAA GTCAAAAGTG AAGAGAAGAT CAAGATGGTA 
GAAAAGTCAG AGAAGGAAAC TGTGATTGTG GAGGAACAGA CAGAGGAGGT CCAAGTGACT 
GAAGAAGTGA CTGAAGAAGA AGAGAAAGAG GCCAAAGAGG AGGAAGGAGA AGCAGTAAAG 
TCTCCCCCAG CAGAAGAGGC TGCATCCCCA GAGAAGGAGG AGGCCAAGTC ACCAGCCGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAGG AGGCCAAGTC CCCACAGAAG 
GAAGAGGCCA AGTCACCAGC TGAGGTGAAG TCCCCTGAGA AGGCCAAGTC CCCCGTGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAAG AAGCCAAATC CCCAGAGAAG 
GCCAAGTCCC CAGTGGAGGC CAAGTCTCCA GAAAAGGCCA AGTCCCCCAT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGT CAAATCCCCA GTGGAGGCCA AATCCCCAGA GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GAGAAAGCCA AGTCCCCTGT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GTGGAAGCCA AGTCCCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCA GTGGAGGCCA AGTCGCCAGA GAAGGCCAAG 
TCTCCTGTGA AGGAGGAGGC CAAGTCCCCA GAGAAGGCCA AGTCTCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT GTGAAAGAGG AGGCCAAGTC CCCAGAGAAG 
GCCAAGTCTC CTGTGAAGGA GGAGGCCAAG TCGCCAGAGA AGGCCAAGTC TCCTGTGAAA 
GAGGAGGCCA AGTCCCCAGA GAAGGCCAAG TCTCCTGTGA AAGAGGAGGC CAAGTCCCCA 
GAGAAGGCCA AGTCCCCAGT AGAGGCCAAG TCCCCAGAGA AGGCCAAGTC TCCTGCGAAG 
GAGGAGGCCA AGTCGCCAGA GAAGGCCAAG TCTCCTGTGA AAGAGGAGGC CAAGTCCCCA 
GAGAAGGCCA AGTCTCCTGT GAAAGAGGAG GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT 
GCGAAGGAGG AGGCCAAGTC CCCAGAGAAG GCCAAGTCCC CAGAGAAGCC CAAAAGCCCT 
GTGAAGGAGG AGGCCAAGTC CCCAGAGAAG CCCAAAAGCC CTGTCAAGGA GGAGGCCAAA 
TCCCCAGAGA AGGCCAAGTC TCCTGTGAAG GATGAGGCCA AGGCTCCTGG GAAGGAGGTC 
CCAAAGAAGG AGGAGGCCAA GTCCCCTGTG AAGGAGGAAG AAAAACCCCA GGAGGTGAGA 
GCCAAAGAGC CCCCAAAGAA GGCAGAGGAA GAGAAAGCTC CAGCCATACC CAAAGCTGAG 
GAGAAGAAGG ACAGCAAGAA AGATGAGGTG CCCAAGAAGG AGGCTCCAAA GCCCGAGGAG 
AAGAAGGAGC CTGCTGTGGA AAAGCCCAAA GAACCCAAAG CTGAAGCCAA GAAGGAAGAG 
GCTGAAGATA AGAAAAAAGC AACCACCCCA GAGAAGGAGG CTCCTGCCAA GGGGAAGGAA 
GAGGCCAAGC CTAAAGAGAA GACTGAGGTG GCCAAGAAGG AACCAGATGA TGCCAAGCCC 
AAAGAACCCA GCAAAGCAGC AGAAAAGCCA AAGAAGGAAG AGACGCCGGC AGCACCGGAG 
AAAAAAGACG CCAAGGAGGG GAAGGCCGCA GAAGCCAAGA AGCCTGAGGA GAAACCCAAA 
ACAGAGGCCC AAGCCAAGGA ACCTGGCAAG GAGGCCTCCA CACCCGGGAA AGCCAAGGCT 
GAGAAATCCT CCAGCACAGA CCAGAAAGAC AGCAGACCTG CAGAGAAGGC CGCAGAAGAT 
AAGGCCACCA AGGGAGAGAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEFH ( XM_005218115.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005218172.1 Bos taurus neurofilament, heavy polypeptide (NEFH), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa28071
Clone ID Related Accession (Same CDS sequence)	XM_010823475.2 , XM_005218115.3 , XM_005218115.2
Accession Version	XM_005218115.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neurofilament heavy polypeptide isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104458.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005218115.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCA GCGGCGCCGA CGCGCTGCTG GGCGCCTTCG CGCCGCTCCA CGGAGGCGGC 
AGCTTGCACT ATGCACTGGC TCGCAAAGGC GGCGCCGGCG GAGCGCGCTC TGCAGCCGGC 
TCCTCCAGCG GATTCCACTC ATGGGCGCGG ACCTCCGTGA GCTCCGTGTC GGCCTCCCCG 
AGCCGCTTCC GAGGCGCCGG GACTACCTCC AGCACCGACT CGCTAGACAC CCTGAGCAAC 
GGACCGGAGG GCTGCGTGGT GGTGGCGCGC AGTGAGAAGG AGCAGCTGCA GGTGCTGAAC 
GACCGCTTCG CGGGCTACAT CGACAAGGTG CGGCAGCTCG AGGCGCATAA CCGCAGCCTG 
GAGGGCGAGG CGGCGGCGCT GCGGCAGCAG CAGGCGGGCC GCGCAGCCAT GGGCGAGCTG 
TACGAGCGCG AGGTGCGCGA GATGCGCGGC GCAGTGCTGC GCTTGGGCGC GGCTCGCGGC 
CAGCTGCGCC TGGAGCAGGA GCACCTGCTG GAGGACATCG CGCACGTGCG CCAGCGCCTG 
GACGACGAGG CCCGGCAGCG CGAGGAGGCC GAGGCAGCGA CGCGCGCACT GGCCCGCTTT 
GCGCAGGAGG CCGAGGCGGC GCGCGTCGAG CTGCAGAAGA AGGCACAAGC CCTGCAGGAA 
GAGTGCGGCT ACCTGCGGCG TCACCACCAG GAGGAGGTGG GCGAGCTGCT CGGCCAGATC 
CAGAGCAGCA GCGCCGCGCA GACGCAGGCC GAGGCGCGCG ATGCCCTCAA GTGCGACGTG 
ACGTCGGCCC TGCGCGAGAT CCGCGCGCAG CTTGAAGGCC ACACGGTGCA GAGCACGCTT 
CAGTCCGAGG AGTGGTTCCG AGTGAGGCTG GACCGACTGT CCGAGGCAGC CAAGGTGAAC 
ACAGACGCCA TGCGCTCAGC ACAGGAGGAG ATATCTGAGT ACCGGCGCCA GCTGCAGGCC 
AGGACCACAG AGCTGGAGAC GCTCAAAAGC ACCAAGGACT CACTGGAGAG GCAGCGCTCT 
GAGCTGGAGG ACCGTCACCA GGCTGACATC GCATCCTACC AGGAGGCCAT CCAGCAGCTG 
GATGCAGAGC TGAGGAACAC CAAGTGGGAG ATGGCAGCCC AGCTCCGAGA GTACCAGGAT 
CTACTCAATG TCAAGATGGC TCTGGACATT GAGATCGCTG CTTACAGAAA ACTCCTGGAG 
GGTGAAGAAT GTCGAATTGG CTTTGGCCCG AGTCCTTTCC TCCTTCCAGA AGGACTCCCC 
AAGATCCCCT CCGTGTCCAC TCACATCAAA GTCAAAAGTG AAGAGAAGAT CAAGATGGTA 
GAAAAGTCAG AGAAGGAAAC TGTGATTGTG GAGGAACAGA CAGAGGAGGT CCAAGTGACT 
GAAGAAGTGA CTGAAGAAGA AGAGAAAGAG GCCAAAGAGG AGGAAGGAGA AGCAGTAAAG 
TCTCCCCCAG CAGAAGAGGC TGCATCCCCA GAGAAGGAGG AGGCCAAGTC ACCAGCCGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAGG AGGCCAAGTC CCCACAGAAG 
GAAGAGGCCA AGTCACCAGC TGAGGTGAAG TCCCCTGAGA AGGCCAAGTC CCCCGTGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAAG AAGCCAAATC CCCAGAGAAG 
GCCAAGTCCC CAGTGGAGGC CAAGTCTCCA GAAAAGGCCA AGTCCCCCAT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGT CAAATCCCCA GTGGAGGCCA AATCCCCAGA GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GAGAAAGCCA AGTCCCCTGT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GTGGAAGCCA AGTCCCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCA GTGGAGGCCA AGTCGCCAGA GAAGGCCAAG 
TCTCCTGTGA AGGAGGAGGC CAAGTCCCCA GAGAAGGCCA AGTCTCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT GTGAAAGAGG AGGCCAAGTC CCCAGAGAAG 
GCCAAGTCTC CTGTGAAGGA GGAGGCCAAG TCGCCAGAGA AGGCCAAGTC TCCTGTGAAA 
GAGGAGGCCA AGTCCCCAGA GAAGGCCAAG TCTCCTGTGA AAGAGGAGGC CAAGTCCCCA 
GAGAAGGCCA AGTCCCCAGT AGAGGCCAAG TCCCCAGAGA AGGCCAAGTC TCCTGCGAAG 
GAGGAGGCCA AGTCGCCAGA GAAGGCCAAG TCTCCTGTGA AAGAGGAGGC CAAGTCCCCA 
GAGAAGGCCA AGTCTCCTGT GAAAGAGGAG GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT 
GCGAAGGAGG AGGCCAAGTC CCCAGAGAAG GCCAAGTCCC CAGAGAAGCC CAAAAGCCCT 
GTGAAGGAGG AGGCCAAGTC CCCAGAGAAG CCCAAAAGCC CTGTCAAGGA GGAGGCCAAA 
TCCCCAGAGA AGGCCAAGTC TCCTGTGAAG GATGAGGCCA AGGCTCCTGG GAAGGAGGTC 
CCAAAGAAGG AGGAGGCCAA GTCCCCTGTG AAGGAGGAAG AAAAACCCCA GGAGGTGAGA 
GCCAAAGAGC CCCCAAAGAA GGCAGAGGAA GAGAAAGCTC CAGCCATACC CAAAGCTGAG 
GAGAAGAAGG ACAGCAAGAA AGATGAGGTG CCCAAGAAGG AGGCTCCAAA GCCCGAGGAG 
AAGAAGGAGC CTGCTGTGGA AAAGCCCAAA GAACCCAAAG CTGAAGCCAA GAAGGAAGAG 
GCTGAAGATA AGAAAAAAGC AACCACCCCA GAGAAGGAGG CTCCTGCCAA GGGGAAGGAA 
GAGGCCAAGC CTAAAGAGAA GACTGAGGTG GCCAAGAAGG AACCAGATGA TGCCAAGCCC 
AAAGAACCCA GCAAAGCAGC AGAAAAGCCA AAGAAGGAAG AGACGCCGGC AGCACCGGAG 
AAAAAAGACG CCAAGGAGGG GAAGGCCGCA GAAGCCAAGA AGCCTGAGGA GAAACCCAAA 
ACAGAGGCCC AAGCCAAGGA ACCTGGCAAG GAGGCCTCCA CACCCGGGAA AGCCAAGGCT 
GAGAAATCCT CCAGCACAGA CCAGAAAGAC AGCAGACCTG CAGAGAAGGC CGCAGAAGAT 
AAGGCCACCA AGGGAGAGAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005218172.1
CDS	79..3282
Translation	MSFSGADALLGAFAPLHGGGSLHYALARKGGAGGARSAAGSSSGFHSWARTSVSSVSASPSRFRGAGTTSSTDSLDTLSNGPEGCVVVARSEKEQLQVLNDRFAGYIDKVRQLEAHNRSLEGEAAALRQQQAGRAAMGELYEREVREMRGAVLRLGAARGQLRLEQEHLLEDIAHVRQRLDDEARQREEAEAATRALARFAQEAEAARVELQKKAQALQEECGYLRRHHQEEVGELLGQIQSSSAAQTQAEARDALKCDVTSALREIRAQLEGHTVQSTLQSEEWFRVRLDRLSEAAKVNTDAMRSAQEEISEYRRQLQARTTELETLKSTKDSLERQRSELEDRHQADIASYQEAIQQLDAELRNTKWEMAAQLREYQDLLNVKMALDIEIAAYRKLLEGEECRIGFGPSPFLLPEGLPKIPSVSTHIKVKSEEKIKMVEKSEKETVIVEEQTEEVQVTEEVTEEEEKEAKEEEGEAVKSPPAEEAASPEKEEAKSPAEAKSPEKAKSPVKEEAKSPQKEEAKSPAEVKSPEKAKSPVEAKSPEKAKSPVKEEAKSPEKAKSPVEAKSPEKAKSPMKEEAKSPEKVKSPVEAKSPEKEEAKSPEKAKSPEKAKSPVKEEAKSPEKAKSPVEAKSPVKEEAKSPEKAKSPVEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVEAKSPEKAKSPAKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPAKEEAKSPEKAKSPEKPKSPVKEEAKSPEKPKSPVKEEAKSPEKAKSPVKDEAKAPGKEVPKKEEAKSPVKEEEKPQEVRAKEPPKKAEEEKAPAIPKAEEKKDSKKDEVPKKEAPKPEEKKEPAVEKPKEPKAEAKKEEAEDKKKATTPEKEAPAKGKEEAKPKEKTEVAKKEPDDAKPKEPSKAAEKPKKEETPAAPEKKDAKEGKAAEAKKPEEKPKTEAQAKEPGKEASTPGKAKAEKSSSTDQKDSRPAEKAAEDKATKGEK

Target ORF information:

RefSeq Version	XM_005218115.2
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofilament, heavy polypeptide (NEFH), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005218115.2

ORF Insert Sequence:

ATGAGCTTCA GCGGCGCCGA CGCGCTGCTG GGCGCCTTCG CGCCGCTCCA CGGAGGCGGC 
AGCTTGCACT ATGCACTGGC TCGCAAAGGC GGCGCCGGCG GAGCGCGCTC TGCAGCCGGC 
TCCTCCAGCG GATTCCACTC ATGGGCGCGG ACCTCCGTGA GCTCCGTGTC GGCCTCCCCG 
AGCCGCTTCC GAGGCGCCGG GACTACCTCC AGCACCGACT CGCTAGACAC CCTGAGCAAC 
GGACCGGAGG GCTGCGTGGT GGTGGCGCGC AGTGAGAAGG AGCAGCTGCA GGTGCTGAAC 
GACCGCTTCG CGGGCTACAT CGACAAGGTG CGGCAGCTCG AGGCGCATAA CCGCAGCCTG 
GAGGGCGAGG CGGCGGCGCT GCGGCAGCAG CAGGCGGGCC GCGCAGCCAT GGGCGAGCTG 
TACGAGCGCG AGGTGCGCGA GATGCGCGGC GCAGTGCTGC GCTTGGGCGC GGCTCGCGGC 
CAGCTGCGCC TGGAGCAGGA GCACCTGCTG GAGGACATCG CGCACGTGCG CCAGCGCCTG 
GACGACGAGG CCCGGCAGCG CGAGGAGGCC GAGGCAGCGA CGCGCGCACT GGCCCGCTTT 
GCGCAGGAGG CCGAGGCGGC GCGCGTCGAG CTGCAGAAGA AGGCACAAGC CCTGCAGGAA 
GAGTGCGGCT ACCTGCGGCG TCACCACCAG GAGGAGGTGG GCGAGCTGCT CGGCCAGATC 
CAGAGCAGCA GCGCCGCGCA GACGCAGGCC GAGGCGCGCG ATGCCCTCAA GTGCGACGTG 
ACGTCGGCCC TGCGCGAGAT CCGCGCGCAG CTTGAAGGCC ACACGGTGCA GAGCACGCTT 
CAGTCCGAGG AGTGGTTCCG AGTGAGGCTG GACCGACTGT CCGAGGCAGC CAAGGTGAAC 
ACAGACGCCA TGCGCTCAGC ACAGGAGGAG ATATCTGAGT ACCGGCGCCA GCTGCAGGCC 
AGGACCACAG AGCTGGAGAC GCTCAAAAGC ACCAAGGACT CACTGGAGAG GCAGCGCTCT 
GAGCTGGAGG ACCGTCACCA GGCTGACATC GCATCCTACC AGGAGGCCAT CCAGCAGCTG 
GATGCAGAGC TGAGGAACAC CAAGTGGGAG ATGGCAGCCC AGCTCCGAGA GTACCAGGAT 
CTACTCAATG TCAAGATGGC TCTGGACATT GAGATCGCTG CTTACAGAAA ACTCCTGGAG 
GGTGAAGAAT GTCGAATTGG CTTTGGCCCG AGTCCTTTCC TCCTTCCAGA AGGACTCCCC 
AAGATCCCCT CCGTGTCCAC TCACATCAAA GTCAAAAGTG AAGAGAAGAT CAAGATGGTA 
GAAAAGTCAG AGAAGGAAAC TGTGATTGTG GAGGAACAGA CAGAGGAGGT CCAAGTGACT 
GAAGAAGTGA CTGAAGAAGA AGAGAAAGAG GCCAAAGAGG AGGAAGGAGA AGCAGTAAAG 
TCTCCCCCAG CAGAAGAGGC TGCATCCCCA GAGAAGGAGG AGGCCAAGTC ACCAGCCGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAGG AGGCCAAGTC CCCACAGAAG 
GAAGAGGCCA AGTCACCAGC TGAGGTGAAG TCCCCTGAGA AGGCCAAGTC CCCCGTGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAAG AAGCCAAATC CCCAGAGAAG 
GCCAAGTCCC CAGTGGAGGC CAAGTCTCCA GAAAAGGCCA AGTCCCCCAT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGT CAAATCCCCA GTGGAGGCCA AATCCCCAGA GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GAGAAAGCCA AGTCCCCTGT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GTGGAAGCCA AGTCCCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCA GTGGAGGCCA AGTCGCCAGA GAAGGCCAAG 
TCTCCTGTGA AGGAGGAGGC CAAGTCCCCA GAGAAGGCCA AGTCTCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT GTGAAAGAGG AGGCCAAGTC CCCAGAGAAG 
GCCAAGTCTC CTGTGAAGGA GGAGGCCAAG TCGCCAGAGA AGGCCAAGTC TCCTGTGAAA 
GAGGAGGCCA AGTCCCCAGA GAAGGCCAAG TCTCCTGTGA AAGAGGAGGC CAAGTCCCCA 
GAGAAGGCCA AGTCCCCAGT AGAGGCCAAG TCCCCAGAGA AGGCCAAGTC TCCTGCGAAG 
GAGGAGGCCA AGTCGCCAGA GAAGGCCAAG TCTCCTGTGA AAGAGGAGGC CAAGTCCCCA 
GAGAAGGCCA AGTCTCCTGT GAAAGAGGAG GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT 
GCGAAGGAGG AGGCCAAGTC CCCAGAGAAG GCCAAGTCCC CAGAGAAGCC CAAAAGCCCT 
GTGAAGGAGG AGGCCAAGTC CCCAGAGAAG CCCAAAAGCC CTGTCAAGGA GGAGGCCAAA 
TCCCCAGAGA AGGCCAAGTC TCCTGTGAAG GATGAGGCCA AGGCTCCTGG GAAGGAGGTC 
CCAAAGAAGG AGGAGGCCAA GTCCCCTGTG AAGGAGGAAG AAAAACCCCA GGAGGTGAGA 
GCCAAAGAGC CCCCAAAGAA GGCAGAGGAA GAGAAAGCTC CAGCCATACC CAAAGCTGAG 
GAGAAGAAGG ACAGCAAGAA AGATGAGGTG CCCAAGAAGG AGGCTCCAAA GCCCGAGGAG 
AAGAAGGAGC CTGCTGTGGA AAAGCCCAAA GAACCCAAAG CTGAAGCCAA GAAGGAAGAG 
GCTGAAGATA AGAAAAAAGC AACCACCCCA GAGAAGGAGG CTCCTGCCAA GGGGAAGGAA 
GAGGCCAAGC CTAAAGAGAA GACTGAGGTG GCCAAGAAGG AACCAGATGA TGCCAAGCCC 
AAAGAACCCA GCAAAGCAGC AGAAAAGCCA AAGAAGGAAG AGACGCCGGC AGCACCGGAG 
AAAAAAGACG CCAAGGAGGG GAAGGCCGCA GAAGCCAAGA AGCCTGAGGA GAAACCCAAA 
ACAGAGGCCC AAGCCAAGGA ACCTGGCAAG GAGGCCTCCA CACCCGGGAA AGCCAAGGCT 
GAGAAATCCT CCAGCACAGA CCAGAAAGAC AGCAGACCTG CAGAGAAGGC CGCAGAAGAT 
AAGGCCACCA AGGGAGAGAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEFH ( XM_010814137.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010812439.1 Bos taurus neurofilament, heavy polypeptide (NEFH), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa28073
Clone ID Related Accession (Same CDS sequence)	XM_010814137.1 , XM_010823476.2
Accession Version	XM_010814137.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3168bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neurofilament heavy polypeptide isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104458.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCA GCGGCGCCGA CGCGCTGCTG GGCGCCTTCG CGCCGCTCCA CGGAGGCGGC 
AGCTTGCACT ATGCACTGGC TCGCAAAGGC GGCGCCGGCG GAGCGCGCTC TGCAGCCGGC 
TCCTCCAGCG GATTCCACTC ATGGGCGCGG ACCTCCGTGA GCTCCGTGTC GGCCTCCCCG 
AGCCGCTTCC GAGGCGCCGG GACTACCTCC AGCACCGACT CGCTAGACAC CCTGAGCAAC 
GGACCGGAGG GCTGCGTGGT GGTGGCGCGC AGTGAGAAGG AGCAGCTGCA GGTGCTGAAC 
GACCGCTTCG CGGGCTACAT CGACAAGGTG CGGCAGCTCG AGGCGCATAA CCGCAGCCTG 
GAGGGCGAGG CGGCGGCGCT GCGGCAGCAG CAGGCGGGCC GCGCAGCCAT GGGCGAGCTG 
TACGAGCGCG AGGTGCGCGA GATGCGCGGC GCAGTGCTGC GCTTGGGCGC GGCTCGCGGC 
CAGCTGCGCC TGGAGCAGGA GCACCTGCTG GAGGACATCG CGCACGTGCG CCAGCGCCTG 
GACGACGAGG CCCGGCAGCG CGAGGAGGCC GAGGCAGCGA CGCGCGCACT GGCCCGCTTT 
GCGCAGGAGG CCGAGGCGGC GCGCGTCGAG CTGCAGAAGA AGGCACAAGC CCTGCAGGAA 
GAGTGCGGCT ACCTGCGGCG TCACCACCAG GAGGAGGTGG GCGAGCTGCT CGGCCAGATC 
CAGAGCAGCA GCGCCGCGCA GACGCAGGCC GAGGCGCGCG ATGCCCTCAA GTGCGACGTG 
ACGTCGGCCC TGCGCGAGAT CCGCGCGCAG CTTGAAGGCC ACACGGTGCA GAGCACGCTT 
CAGTCCGAGG AGTGGTTCCG AGTGAGGCTG GACCGACTGT CCGAGGCAGC CAAGGTGAAC 
ACAGACGCCA TGCGCTCAGC ACAGGAGGAG ATATCTGAGT ACCGGCGCCA GCTGCAGGCC 
AGGACCACAG AGCTGGAGAC GCTCAAAAGC ACCAAGGACT CACTGGAGAG GCAGCGCTCT 
GAGCTGGAGG ACCGTCACCA GGCTGACATC GCATCCTACC AGGAGGCCAT CCAGCAGCTG 
GATGCAGAGC TGAGGAACAC CAAGTGGGAG ATGGCAGCCC AGCTCCGAGA GTACCAGGAT 
CTACTCAATG TCAAGATGGC TCTGGACATT GAGATCGCTG CTTACAGAAA ACTCCTGGAG 
GGTGAAGAAT GTCGAATTGG CTTTGGCCCG AGTCCTTTCC TCCTTCCAGA AGGACTCCCC 
AAGATCCCCT CCGTGTCCAC TCACATCAAA GTCAAAAGTG AAGAGAAGAT CAAGATGGTA 
GAAAAGTCAG AGAAGGAAAC TGTGATTGTG GAGGAACAGA CAGAGGAGGT CCAAGTGACT 
GAAGAAGTGA CTGAAGAAGA AGAGAAAGAG GCCAAAGAGG AGGAAGGAGA AGCAGTAAAG 
TCTCCCCCAG CAGAAGAGGC TGCATCCCCA GAGAAGGAGG AGGCCAAGTC ACCAGCCGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAGG AGGCCAAGTC CCCACAGAAG 
GAAGAGGCCA AGTCACCAGC TGAGGTGAAG TCCCCTGAGA AGGCCAAGTC CCCCGTGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAAG AAGCCAAATC CCCAGAGAAG 
GCCAAGTCCC CAGTGGAGGC CAAGTCTCCA GAAAAGGCCA AGTCCCCCAT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGT CAAATCCCCA GTGGAGGCCA AATCCCCAGA GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GAGAAAGCCA AGTCCCCTGT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GTGGAAGCCA AGTCCCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCA GTGGAGGCCA AGTCGCCAGA GAAGGCCAAG 
TCTCCTGTGA AGGAGGAGGC CAAGTCCCCA GAGAAGGCCA AGTCTCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT GTGAAAGAGG AGGCCAAGTC CCCAGAGAAG 
GCCAAGTCTC CTGTGAAGGA GGAGGCCAAG TCGCCAGAGA AGGCCAAGTC TCCTGTGAAA 
GAGGAGGCCA AGTCCCCAGA GAAGGCCAAG TCTCCTGTGA AAGAGGAGGC CAAGTCCCCA 
GAGAAGGCCA AGTCTCCTGT GAAAGAGGAG GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT 
GTGAAAGAGG AGGCCAAGTC CCCAGAGAAG GCCAAGTCTC CTGCGAAGGA GGAGGCCAAG 
TCCCCAGAGA AGGCCAAGTC CCCAGAGAAG CCCAAAAGCC CTGTGAAGGA GGAGGCCAAG 
TCCCCAGAGA AGCCCAAAAG CCCTGTGAAG GAGGAGGCCA AGTCCCCAGA GAAGCCCAAA 
AGCCCTGTCA AGGAGGAGGC CAAATCCCCA GAGAAGGCCA AGTCTCCTGT GAAGGATGAG 
GCCAAGGCTC CTGGGAAGGA GGTCCCAAAG AAGGAGGAGG CCAAGTCCCC TGTGAAGGAG 
GAAGAAAAAC CCCAGGAGGT GAGAGCCAAA GAGCCCCCAA AGAAGGCAGA GGAAGAGAAA 
GCTCCAGCCA TACCCAAAGC TGAGGAGAAG AAGGACAGCA AGAAAGATGA GGTGCCCAAG 
AAGGAGGCTC CAAAGCCCGA GGAGAAGAAG GAGCCTGCTG TGGAAAAGCC CAAAGAACCC 
AAAGCTGAAG CCAAGAAGGA AGAGGCTGAA GATAAGAAAA AAGCAACCAC CCCAGAGAAG 
GAGGCTCCTG CCAAGGGGAA GGAAGAGGCC AAGCCTAAAG AGAAGACTGA GGTGGCCAAG 
AAGGAACCAG ATGATGCCAA GCCCAAAGAA CCCAGCAAAG CAGCAGAAAA GCCAAAGAAG 
GAAGAGACGC CGGCAGCACC GGAGAAAAAA GACGCCAAGG AGGGGAAGGC CGCAGAAGCC 
AAGAAGCCTG AGGAGAAACC CAAAACAGAG GCCCAAGCCA AGGAACCTGG CAAGGAGGCC 
TCCACACCCG GGAAAGCCAA GGCTGAGAAA TCCTCCAGCA CAGACCAGAA AGACAGCAGA 
CCTGCAGAGA AGGCCGCAGA AGATAAGGCC ACCAAGGGAG AGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010812439.1
CDS	79..3246
Translation	MSFSGADALLGAFAPLHGGGSLHYALARKGGAGGARSAAGSSSGFHSWARTSVSSVSASPSRFRGAGTTSSTDSLDTLSNGPEGCVVVARSEKEQLQVLNDRFAGYIDKVRQLEAHNRSLEGEAAALRQQQAGRAAMGELYEREVREMRGAVLRLGAARGQLRLEQEHLLEDIAHVRQRLDDEARQREEAEAATRALARFAQEAEAARVELQKKAQALQEECGYLRRHHQEEVGELLGQIQSSSAAQTQAEARDALKCDVTSALREIRAQLEGHTVQSTLQSEEWFRVRLDRLSEAAKVNTDAMRSAQEEISEYRRQLQARTTELETLKSTKDSLERQRSELEDRHQADIASYQEAIQQLDAELRNTKWEMAAQLREYQDLLNVKMALDIEIAAYRKLLEGEECRIGFGPSPFLLPEGLPKIPSVSTHIKVKSEEKIKMVEKSEKETVIVEEQTEEVQVTEEVTEEEEKEAKEEEGEAVKSPPAEEAASPEKEEAKSPAEAKSPEKAKSPVKEEAKSPQKEEAKSPAEVKSPEKAKSPVEAKSPEKAKSPVKEEAKSPEKAKSPVEAKSPEKAKSPMKEEAKSPEKVKSPVEAKSPEKEEAKSPEKAKSPEKAKSPVKEEAKSPEKAKSPVEAKSPVKEEAKSPEKAKSPVEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPAKEEAKSPEKAKSPEKPKSPVKEEAKSPEKPKSPVKEEAKSPEKPKSPVKEEAKSPEKAKSPVKDEAKAPGKEVPKKEEAKSPVKEEEKPQEVRAKEPPKKAEEEKAPAIPKAEEKKDSKKDEVPKKEAPKPEEKKEPAVEKPKEPKAEAKKEEAEDKKKATTPEKEAPAKGKEEAKPKEKTEVAKKEPDDAKPKEPSKAAEKPKKEETPAAPEKKDAKEGKAAEAKKPEEKPKTEAQAKEPGKEASTPGKAKAEKSSSTDQKDSRPAEKAAEDKATKGEK

Target ORF information:

RefSeq Version	XM_010814137.1
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofilament, heavy polypeptide (NEFH), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010814137.1

ORF Insert Sequence:

ATGAGCTTCA GCGGCGCCGA CGCGCTGCTG GGCGCCTTCG CGCCGCTCCA CGGAGGCGGC 
AGCTTGCACT ATGCACTGGC TCGCAAAGGC GGCGCCGGCG GAGCGCGCTC TGCAGCCGGC 
TCCTCCAGCG GATTCCACTC ATGGGCGCGG ACCTCCGTGA GCTCCGTGTC GGCCTCCCCG 
AGCCGCTTCC GAGGCGCCGG GACTACCTCC AGCACCGACT CGCTAGACAC CCTGAGCAAC 
GGACCGGAGG GCTGCGTGGT GGTGGCGCGC AGTGAGAAGG AGCAGCTGCA GGTGCTGAAC 
GACCGCTTCG CGGGCTACAT CGACAAGGTG CGGCAGCTCG AGGCGCATAA CCGCAGCCTG 
GAGGGCGAGG CGGCGGCGCT GCGGCAGCAG CAGGCGGGCC GCGCAGCCAT GGGCGAGCTG 
TACGAGCGCG AGGTGCGCGA GATGCGCGGC GCAGTGCTGC GCTTGGGCGC GGCTCGCGGC 
CAGCTGCGCC TGGAGCAGGA GCACCTGCTG GAGGACATCG CGCACGTGCG CCAGCGCCTG 
GACGACGAGG CCCGGCAGCG CGAGGAGGCC GAGGCAGCGA CGCGCGCACT GGCCCGCTTT 
GCGCAGGAGG CCGAGGCGGC GCGCGTCGAG CTGCAGAAGA AGGCACAAGC CCTGCAGGAA 
GAGTGCGGCT ACCTGCGGCG TCACCACCAG GAGGAGGTGG GCGAGCTGCT CGGCCAGATC 
CAGAGCAGCA GCGCCGCGCA GACGCAGGCC GAGGCGCGCG ATGCCCTCAA GTGCGACGTG 
ACGTCGGCCC TGCGCGAGAT CCGCGCGCAG CTTGAAGGCC ACACGGTGCA GAGCACGCTT 
CAGTCCGAGG AGTGGTTCCG AGTGAGGCTG GACCGACTGT CCGAGGCAGC CAAGGTGAAC 
ACAGACGCCA TGCGCTCAGC ACAGGAGGAG ATATCTGAGT ACCGGCGCCA GCTGCAGGCC 
AGGACCACAG AGCTGGAGAC GCTCAAAAGC ACCAAGGACT CACTGGAGAG GCAGCGCTCT 
GAGCTGGAGG ACCGTCACCA GGCTGACATC GCATCCTACC AGGAGGCCAT CCAGCAGCTG 
GATGCAGAGC TGAGGAACAC CAAGTGGGAG ATGGCAGCCC AGCTCCGAGA GTACCAGGAT 
CTACTCAATG TCAAGATGGC TCTGGACATT GAGATCGCTG CTTACAGAAA ACTCCTGGAG 
GGTGAAGAAT GTCGAATTGG CTTTGGCCCG AGTCCTTTCC TCCTTCCAGA AGGACTCCCC 
AAGATCCCCT CCGTGTCCAC TCACATCAAA GTCAAAAGTG AAGAGAAGAT CAAGATGGTA 
GAAAAGTCAG AGAAGGAAAC TGTGATTGTG GAGGAACAGA CAGAGGAGGT CCAAGTGACT 
GAAGAAGTGA CTGAAGAAGA AGAGAAAGAG GCCAAAGAGG AGGAAGGAGA AGCAGTAAAG 
TCTCCCCCAG CAGAAGAGGC TGCATCCCCA GAGAAGGAGG AGGCCAAGTC ACCAGCCGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAGG AGGCCAAGTC CCCACAGAAG 
GAAGAGGCCA AGTCACCAGC TGAGGTGAAG TCCCCTGAGA AGGCCAAGTC CCCCGTGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAAG AAGCCAAATC CCCAGAGAAG 
GCCAAGTCCC CAGTGGAGGC CAAGTCTCCA GAAAAGGCCA AGTCCCCCAT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGT CAAATCCCCA GTGGAGGCCA AATCCCCAGA GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GAGAAAGCCA AGTCCCCTGT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GTGGAAGCCA AGTCCCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCA GTGGAGGCCA AGTCGCCAGA GAAGGCCAAG 
TCTCCTGTGA AGGAGGAGGC CAAGTCCCCA GAGAAGGCCA AGTCTCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT GTGAAAGAGG AGGCCAAGTC CCCAGAGAAG 
GCCAAGTCTC CTGTGAAGGA GGAGGCCAAG TCGCCAGAGA AGGCCAAGTC TCCTGTGAAA 
GAGGAGGCCA AGTCCCCAGA GAAGGCCAAG TCTCCTGTGA AAGAGGAGGC CAAGTCCCCA 
GAGAAGGCCA AGTCTCCTGT GAAAGAGGAG GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT 
GTGAAAGAGG AGGCCAAGTC CCCAGAGAAG GCCAAGTCTC CTGCGAAGGA GGAGGCCAAG 
TCCCCAGAGA AGGCCAAGTC CCCAGAGAAG CCCAAAAGCC CTGTGAAGGA GGAGGCCAAG 
TCCCCAGAGA AGCCCAAAAG CCCTGTGAAG GAGGAGGCCA AGTCCCCAGA GAAGCCCAAA 
AGCCCTGTCA AGGAGGAGGC CAAATCCCCA GAGAAGGCCA AGTCTCCTGT GAAGGATGAG 
GCCAAGGCTC CTGGGAAGGA GGTCCCAAAG AAGGAGGAGG CCAAGTCCCC TGTGAAGGAG 
GAAGAAAAAC CCCAGGAGGT GAGAGCCAAA GAGCCCCCAA AGAAGGCAGA GGAAGAGAAA 
GCTCCAGCCA TACCCAAAGC TGAGGAGAAG AAGGACAGCA AGAAAGATGA GGTGCCCAAG 
AAGGAGGCTC CAAAGCCCGA GGAGAAGAAG GAGCCTGCTG TGGAAAAGCC CAAAGAACCC 
AAAGCTGAAG CCAAGAAGGA AGAGGCTGAA GATAAGAAAA AAGCAACCAC CCCAGAGAAG 
GAGGCTCCTG CCAAGGGGAA GGAAGAGGCC AAGCCTAAAG AGAAGACTGA GGTGGCCAAG 
AAGGAACCAG ATGATGCCAA GCCCAAAGAA CCCAGCAAAG CAGCAGAAAA GCCAAAGAAG 
GAAGAGACGC CGGCAGCACC GGAGAAAAAA GACGCCAAGG AGGGGAAGGC CGCAGAAGCC 
AAGAAGCCTG AGGAGAAACC CAAAACAGAG GCCCAAGCCA AGGAACCTGG CAAGGAGGCC 
TCCACACCCG GGAAAGCCAA GGCTGAGAAA TCCTCCAGCA CAGACCAGAA AGACAGCAGA 
CCTGCAGAGA AGGCCGCAGA AGATAAGGCC ACCAAGGGAG AGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEFH ( XM_010823476.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010821778.2 Bos taurus neurofilament, heavy polypeptide (NEFH), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa28073
Clone ID Related Accession (Same CDS sequence)	XM_010814137.1 , XM_010823476.2
Accession Version	XM_010823476.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3168bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neurofilament heavy polypeptide isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646373.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010823476.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCA GCGGCGCCGA CGCGCTGCTG GGCGCCTTCG CGCCGCTCCA CGGAGGCGGC 
AGCTTGCACT ATGCACTGGC TCGCAAAGGC GGCGCCGGCG GAGCGCGCTC TGCAGCCGGC 
TCCTCCAGCG GATTCCACTC ATGGGCGCGG ACCTCCGTGA GCTCCGTGTC GGCCTCCCCG 
AGCCGCTTCC GAGGCGCCGG GACTACCTCC AGCACCGACT CGCTAGACAC CCTGAGCAAC 
GGACCGGAGG GCTGCGTGGT GGTGGCGCGC AGTGAGAAGG AGCAGCTGCA GGTGCTGAAC 
GACCGCTTCG CGGGCTACAT CGACAAGGTG CGGCAGCTCG AGGCGCATAA CCGCAGCCTG 
GAGGGCGAGG CGGCGGCGCT GCGGCAGCAG CAGGCGGGCC GCGCAGCCAT GGGCGAGCTG 
TACGAGCGCG AGGTGCGCGA GATGCGCGGC GCAGTGCTGC GCTTGGGCGC GGCTCGCGGC 
CAGCTGCGCC TGGAGCAGGA GCACCTGCTG GAGGACATCG CGCACGTGCG CCAGCGCCTG 
GACGACGAGG CCCGGCAGCG CGAGGAGGCC GAGGCAGCGA CGCGCGCACT GGCCCGCTTT 
GCGCAGGAGG CCGAGGCGGC GCGCGTCGAG CTGCAGAAGA AGGCACAAGC CCTGCAGGAA 
GAGTGCGGCT ACCTGCGGCG TCACCACCAG GAGGAGGTGG GCGAGCTGCT CGGCCAGATC 
CAGAGCAGCA GCGCCGCGCA GACGCAGGCC GAGGCGCGCG ATGCCCTCAA GTGCGACGTG 
ACGTCGGCCC TGCGCGAGAT CCGCGCGCAG CTTGAAGGCC ACACGGTGCA GAGCACGCTT 
CAGTCCGAGG AGTGGTTCCG AGTGAGGCTG GACCGACTGT CCGAGGCAGC CAAGGTGAAC 
ACAGACGCCA TGCGCTCAGC ACAGGAGGAG ATATCTGAGT ACCGGCGCCA GCTGCAGGCC 
AGGACCACAG AGCTGGAGAC GCTCAAAAGC ACCAAGGACT CACTGGAGAG GCAGCGCTCT 
GAGCTGGAGG ACCGTCACCA GGCTGACATC GCATCCTACC AGGAGGCCAT CCAGCAGCTG 
GATGCAGAGC TGAGGAACAC CAAGTGGGAG ATGGCAGCCC AGCTCCGAGA GTACCAGGAT 
CTACTCAATG TCAAGATGGC TCTGGACATT GAGATCGCTG CTTACAGAAA ACTCCTGGAG 
GGTGAAGAAT GTCGAATTGG CTTTGGCCCG AGTCCTTTCC TCCTTCCAGA AGGACTCCCC 
AAGATCCCCT CCGTGTCCAC TCACATCAAA GTCAAAAGTG AAGAGAAGAT CAAGATGGTA 
GAAAAGTCAG AGAAGGAAAC TGTGATTGTG GAGGAACAGA CAGAGGAGGT CCAAGTGACT 
GAAGAAGTGA CTGAAGAAGA AGAGAAAGAG GCCAAAGAGG AGGAAGGAGA AGCAGTAAAG 
TCTCCCCCAG CAGAAGAGGC TGCATCCCCA GAGAAGGAGG AGGCCAAGTC ACCAGCCGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAGG AGGCCAAGTC CCCACAGAAG 
GAAGAGGCCA AGTCACCAGC TGAGGTGAAG TCCCCTGAGA AGGCCAAGTC CCCCGTGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAAG AAGCCAAATC CCCAGAGAAG 
GCCAAGTCCC CAGTGGAGGC CAAGTCTCCA GAAAAGGCCA AGTCCCCCAT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGT CAAATCCCCA GTGGAGGCCA AATCCCCAGA GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GAGAAAGCCA AGTCCCCTGT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GTGGAAGCCA AGTCCCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCA GTGGAGGCCA AGTCGCCAGA GAAGGCCAAG 
TCTCCTGTGA AGGAGGAGGC CAAGTCCCCA GAGAAGGCCA AGTCTCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT GTGAAAGAGG AGGCCAAGTC CCCAGAGAAG 
GCCAAGTCTC CTGTGAAGGA GGAGGCCAAG TCGCCAGAGA AGGCCAAGTC TCCTGTGAAA 
GAGGAGGCCA AGTCCCCAGA GAAGGCCAAG TCTCCTGTGA AAGAGGAGGC CAAGTCCCCA 
GAGAAGGCCA AGTCTCCTGT GAAAGAGGAG GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT 
GTGAAAGAGG AGGCCAAGTC CCCAGAGAAG GCCAAGTCTC CTGCGAAGGA GGAGGCCAAG 
TCCCCAGAGA AGGCCAAGTC CCCAGAGAAG CCCAAAAGCC CTGTGAAGGA GGAGGCCAAG 
TCCCCAGAGA AGCCCAAAAG CCCTGTGAAG GAGGAGGCCA AGTCCCCAGA GAAGCCCAAA 
AGCCCTGTCA AGGAGGAGGC CAAATCCCCA GAGAAGGCCA AGTCTCCTGT GAAGGATGAG 
GCCAAGGCTC CTGGGAAGGA GGTCCCAAAG AAGGAGGAGG CCAAGTCCCC TGTGAAGGAG 
GAAGAAAAAC CCCAGGAGGT GAGAGCCAAA GAGCCCCCAA AGAAGGCAGA GGAAGAGAAA 
GCTCCAGCCA TACCCAAAGC TGAGGAGAAG AAGGACAGCA AGAAAGATGA GGTGCCCAAG 
AAGGAGGCTC CAAAGCCCGA GGAGAAGAAG GAGCCTGCTG TGGAAAAGCC CAAAGAACCC 
AAAGCTGAAG CCAAGAAGGA AGAGGCTGAA GATAAGAAAA AAGCAACCAC CCCAGAGAAG 
GAGGCTCCTG CCAAGGGGAA GGAAGAGGCC AAGCCTAAAG AGAAGACTGA GGTGGCCAAG 
AAGGAACCAG ATGATGCCAA GCCCAAAGAA CCCAGCAAAG CAGCAGAAAA GCCAAAGAAG 
GAAGAGACGC CGGCAGCACC GGAGAAAAAA GACGCCAAGG AGGGGAAGGC CGCAGAAGCC 
AAGAAGCCTG AGGAGAAACC CAAAACAGAG GCCCAAGCCA AGGAACCTGG CAAGGAGGCC 
TCCACACCCG GGAAAGCCAA GGCTGAGAAA TCCTCCAGCA CAGACCAGAA AGACAGCAGA 
CCTGCAGAGA AGGCCGCAGA AGATAAGGCC ACCAAGGGAG AGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010821778.2
CDS	79..3246
Translation	MSFSGADALLGAFAPLHGGGSLHYALARKGGAGGARSAAGSSSGFHSWARTSVSSVSASPSRFRGAGTTSSTDSLDTLSNGPEGCVVVARSEKEQLQVLNDRFAGYIDKVRQLEAHNRSLEGEAAALRQQQAGRAAMGELYEREVREMRGAVLRLGAARGQLRLEQEHLLEDIAHVRQRLDDEARQREEAEAATRALARFAQEAEAARVELQKKAQALQEECGYLRRHHQEEVGELLGQIQSSSAAQTQAEARDALKCDVTSALREIRAQLEGHTVQSTLQSEEWFRVRLDRLSEAAKVNTDAMRSAQEEISEYRRQLQARTTELETLKSTKDSLERQRSELEDRHQADIASYQEAIQQLDAELRNTKWEMAAQLREYQDLLNVKMALDIEIAAYRKLLEGEECRIGFGPSPFLLPEGLPKIPSVSTHIKVKSEEKIKMVEKSEKETVIVEEQTEEVQVTEEVTEEEEKEAKEEEGEAVKSPPAEEAASPEKEEAKSPAEAKSPEKAKSPVKEEAKSPQKEEAKSPAEVKSPEKAKSPVEAKSPEKAKSPVKEEAKSPEKAKSPVEAKSPEKAKSPMKEEAKSPEKVKSPVEAKSPEKEEAKSPEKAKSPEKAKSPVKEEAKSPEKAKSPVEAKSPVKEEAKSPEKAKSPVEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPAKEEAKSPEKAKSPEKPKSPVKEEAKSPEKPKSPVKEEAKSPEKPKSPVKEEAKSPEKAKSPVKDEAKAPGKEVPKKEEAKSPVKEEEKPQEVRAKEPPKKAEEEKAPAIPKAEEKKDSKKDEVPKKEAPKPEEKKEPAVEKPKEPKAEAKKEEAEDKKKATTPEKEAPAKGKEEAKPKEKTEVAKKEPDDAKPKEPSKAAEKPKKEETPAAPEKKDAKEGKAAEAKKPEEKPKTEAQAKEPGKEASTPGKAKAEKSSSTDQKDSRPAEKAAEDKATKGEK

Target ORF information:

RefSeq Version	XM_010823476.2
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofilament, heavy polypeptide (NEFH), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010823476.2

ORF Insert Sequence:

ATGAGCTTCA GCGGCGCCGA CGCGCTGCTG GGCGCCTTCG CGCCGCTCCA CGGAGGCGGC 
AGCTTGCACT ATGCACTGGC TCGCAAAGGC GGCGCCGGCG GAGCGCGCTC TGCAGCCGGC 
TCCTCCAGCG GATTCCACTC ATGGGCGCGG ACCTCCGTGA GCTCCGTGTC GGCCTCCCCG 
AGCCGCTTCC GAGGCGCCGG GACTACCTCC AGCACCGACT CGCTAGACAC CCTGAGCAAC 
GGACCGGAGG GCTGCGTGGT GGTGGCGCGC AGTGAGAAGG AGCAGCTGCA GGTGCTGAAC 
GACCGCTTCG CGGGCTACAT CGACAAGGTG CGGCAGCTCG AGGCGCATAA CCGCAGCCTG 
GAGGGCGAGG CGGCGGCGCT GCGGCAGCAG CAGGCGGGCC GCGCAGCCAT GGGCGAGCTG 
TACGAGCGCG AGGTGCGCGA GATGCGCGGC GCAGTGCTGC GCTTGGGCGC GGCTCGCGGC 
CAGCTGCGCC TGGAGCAGGA GCACCTGCTG GAGGACATCG CGCACGTGCG CCAGCGCCTG 
GACGACGAGG CCCGGCAGCG CGAGGAGGCC GAGGCAGCGA CGCGCGCACT GGCCCGCTTT 
GCGCAGGAGG CCGAGGCGGC GCGCGTCGAG CTGCAGAAGA AGGCACAAGC CCTGCAGGAA 
GAGTGCGGCT ACCTGCGGCG TCACCACCAG GAGGAGGTGG GCGAGCTGCT CGGCCAGATC 
CAGAGCAGCA GCGCCGCGCA GACGCAGGCC GAGGCGCGCG ATGCCCTCAA GTGCGACGTG 
ACGTCGGCCC TGCGCGAGAT CCGCGCGCAG CTTGAAGGCC ACACGGTGCA GAGCACGCTT 
CAGTCCGAGG AGTGGTTCCG AGTGAGGCTG GACCGACTGT CCGAGGCAGC CAAGGTGAAC 
ACAGACGCCA TGCGCTCAGC ACAGGAGGAG ATATCTGAGT ACCGGCGCCA GCTGCAGGCC 
AGGACCACAG AGCTGGAGAC GCTCAAAAGC ACCAAGGACT CACTGGAGAG GCAGCGCTCT 
GAGCTGGAGG ACCGTCACCA GGCTGACATC GCATCCTACC AGGAGGCCAT CCAGCAGCTG 
GATGCAGAGC TGAGGAACAC CAAGTGGGAG ATGGCAGCCC AGCTCCGAGA GTACCAGGAT 
CTACTCAATG TCAAGATGGC TCTGGACATT GAGATCGCTG CTTACAGAAA ACTCCTGGAG 
GGTGAAGAAT GTCGAATTGG CTTTGGCCCG AGTCCTTTCC TCCTTCCAGA AGGACTCCCC 
AAGATCCCCT CCGTGTCCAC TCACATCAAA GTCAAAAGTG AAGAGAAGAT CAAGATGGTA 
GAAAAGTCAG AGAAGGAAAC TGTGATTGTG GAGGAACAGA CAGAGGAGGT CCAAGTGACT 
GAAGAAGTGA CTGAAGAAGA AGAGAAAGAG GCCAAAGAGG AGGAAGGAGA AGCAGTAAAG 
TCTCCCCCAG CAGAAGAGGC TGCATCCCCA GAGAAGGAGG AGGCCAAGTC ACCAGCCGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAGG AGGCCAAGTC CCCACAGAAG 
GAAGAGGCCA AGTCACCAGC TGAGGTGAAG TCCCCTGAGA AGGCCAAGTC CCCCGTGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAAG AAGCCAAATC CCCAGAGAAG 
GCCAAGTCCC CAGTGGAGGC CAAGTCTCCA GAAAAGGCCA AGTCCCCCAT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGT CAAATCCCCA GTGGAGGCCA AATCCCCAGA GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GAGAAAGCCA AGTCCCCTGT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GTGGAAGCCA AGTCCCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCA GTGGAGGCCA AGTCGCCAGA GAAGGCCAAG 
TCTCCTGTGA AGGAGGAGGC CAAGTCCCCA GAGAAGGCCA AGTCTCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT GTGAAAGAGG AGGCCAAGTC CCCAGAGAAG 
GCCAAGTCTC CTGTGAAGGA GGAGGCCAAG TCGCCAGAGA AGGCCAAGTC TCCTGTGAAA 
GAGGAGGCCA AGTCCCCAGA GAAGGCCAAG TCTCCTGTGA AAGAGGAGGC CAAGTCCCCA 
GAGAAGGCCA AGTCTCCTGT GAAAGAGGAG GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT 
GTGAAAGAGG AGGCCAAGTC CCCAGAGAAG GCCAAGTCTC CTGCGAAGGA GGAGGCCAAG 
TCCCCAGAGA AGGCCAAGTC CCCAGAGAAG CCCAAAAGCC CTGTGAAGGA GGAGGCCAAG 
TCCCCAGAGA AGCCCAAAAG CCCTGTGAAG GAGGAGGCCA AGTCCCCAGA GAAGCCCAAA 
AGCCCTGTCA AGGAGGAGGC CAAATCCCCA GAGAAGGCCA AGTCTCCTGT GAAGGATGAG 
GCCAAGGCTC CTGGGAAGGA GGTCCCAAAG AAGGAGGAGG CCAAGTCCCC TGTGAAGGAG 
GAAGAAAAAC CCCAGGAGGT GAGAGCCAAA GAGCCCCCAA AGAAGGCAGA GGAAGAGAAA 
GCTCCAGCCA TACCCAAAGC TGAGGAGAAG AAGGACAGCA AGAAAGATGA GGTGCCCAAG 
AAGGAGGCTC CAAAGCCCGA GGAGAAGAAG GAGCCTGCTG TGGAAAAGCC CAAAGAACCC 
AAAGCTGAAG CCAAGAAGGA AGAGGCTGAA GATAAGAAAA AAGCAACCAC CCCAGAGAAG 
GAGGCTCCTG CCAAGGGGAA GGAAGAGGCC AAGCCTAAAG AGAAGACTGA GGTGGCCAAG 
AAGGAACCAG ATGATGCCAA GCCCAAAGAA CCCAGCAAAG CAGCAGAAAA GCCAAAGAAG 
GAAGAGACGC CGGCAGCACC GGAGAAAAAA GACGCCAAGG AGGGGAAGGC CGCAGAAGCC 
AAGAAGCCTG AGGAGAAACC CAAAACAGAG GCCCAAGCCA AGGAACCTGG CAAGGAGGCC 
TCCACACCCG GGAAAGCCAA GGCTGAGAAA TCCTCCAGCA CAGACCAGAA AGACAGCAGA 
CCTGCAGAGA AGGCCGCAGA AGATAAGGCC ACCAAGGGAG AGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEFH ( XM_005218119.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005218176.1 Bos taurus neurofilament, heavy polypeptide (NEFH), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa28075
Clone ID Related Accession (Same CDS sequence)	XM_005218119.2 , XM_010823477.2
Accession Version	XM_005218119.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3120bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neurofilament heavy polypeptide isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104458.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005218119.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCA GCGGCGCCGA CGCGCTGCTG GGCGCCTTCG CGCCGCTCCA CGGAGGCGGC 
AGCTTGCACT ATGCACTGGC TCGCAAAGGC GGCGCCGGCG GAGCGCGCTC TGCAGCCGGC 
TCCTCCAGCG GATTCCACTC ATGGGCGCGG ACCTCCGTGA GCTCCGTGTC GGCCTCCCCG 
AGCCGCTTCC GAGGCGCCGG GACTACCTCC AGCACCGACT CGCTAGACAC CCTGAGCAAC 
GGACCGGAGG GCTGCGTGGT GGTGGCGCGC AGTGAGAAGG AGCAGCTGCA GGTGCTGAAC 
GACCGCTTCG CGGGCTACAT CGACAAGGTG CGGCAGCTCG AGGCGCATAA CCGCAGCCTG 
GAGGGCGAGG CGGCGGCGCT GCGGCAGCAG CAGGCGGGCC GCGCAGCCAT GGGCGAGCTG 
TACGAGCGCG AGGTGCGCGA GATGCGCGGC GCAGTGCTGC GCTTGGGCGC GGCTCGCGGC 
CAGCTGCGCC TGGAGCAGGA GCACCTGCTG GAGGACATCG CGCACGTGCG CCAGCGCCTG 
GACGACGAGG CCCGGCAGCG CGAGGAGGCC GAGGCAGCGA CGCGCGCACT GGCCCGCTTT 
GCGCAGGAGG CCGAGGCGGC GCGCGTCGAG CTGCAGAAGA AGGCACAAGC CCTGCAGGAA 
GAGTGCGGCT ACCTGCGGCG TCACCACCAG GAGGAGGTGG GCGAGCTGCT CGGCCAGATC 
CAGAGCAGCA GCGCCGCGCA GACGCAGGCC GAGGCGCGCG ATGCCCTCAA GTGCGACGTG 
ACGTCGGCCC TGCGCGAGAT CCGCGCGCAG CTTGAAGGCC ACACGGTGCA GAGCACGCTT 
CAGTCCGAGG AGTGGTTCCG AGTGAGGCTG GACCGACTGT CCGAGGCAGC CAAGGTGAAC 
ACAGACGCCA TGCGCTCAGC ACAGGAGGAG ATATCTGAGT ACCGGCGCCA GCTGCAGGCC 
AGGACCACAG AGCTGGAGAC GCTCAAAAGC ACCAAGGACT CACTGGAGAG GCAGCGCTCT 
GAGCTGGAGG ACCGTCACCA GGCTGACATC GCATCCTACC AGGAGGCCAT CCAGCAGCTG 
GATGCAGAGC TGAGGAACAC CAAGTGGGAG ATGGCAGCCC AGCTCCGAGA GTACCAGGAT 
CTACTCAATG TCAAGATGGC TCTGGACATT GAGATCGCTG CTTACAGAAA ACTCCTGGAG 
GGTGAAGAAT GTCGAATTGG CTTTGGCCCG AGTCCTTTCC TCCTTCCAGA AGGACTCCCC 
AAGATCCCCT CCGTGTCCAC TCACATCAAA GTCAAAAGTG AAGAGAAGAT CAAGATGGTA 
GAAAAGTCAG AGAAGGAAAC TGTGATTGTG GAGGAACAGA CAGAGGAGGT CCAAGTGACT 
GAAGAAGTGA CTGAAGAAGA AGAGAAAGAG GCCAAAGAGG AGGAAGGAGA AGCAGTAAAG 
TCTCCCCCAG CAGAAGAGGC TGCATCCCCA GAGAAGGAGG AGGCCAAGTC ACCAGCCGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAGG AGGCCAAGTC CCCACAGAAG 
GAAGAGGCCA AGTCACCAGC TGAGGTGAAG TCCCCTGAGA AGGCCAAGTC CCCCGTGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAAG AAGCCAAATC CCCAGAGAAG 
GCCAAGTCCC CAGTGGAGGC CAAGTCTCCA GAAAAGGCCA AGTCCCCCAT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGT CAAATCCCCA GTGGAGGCCA AATCCCCAGA GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GAGAAAGCCA AGTCCCCTGT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GTGGAAGCCA AGTCCCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCA GTGGAGGCCA AGTCGCCAGA GAAGGCCAAG 
TCTCCTGTGA AGGAGGAGGC CAAGTCCCCA GAGAAGGCCA AGTCTCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT GTGAAAGAGG AGGCCAAGTC CCCAGAGAAG 
GCCAAGTCTC CTGTGAAGGA GGAGGCCAAG TCGCCAGAGA AGGCCAAGTC TCCTGTGAAA 
GAGGAGGCCA AGTCCCCAGA GAAGGCCAAG TCTCCTGTGA AAGAGGAGGC CAAGTCCCCA 
GAGAAGGCCA AGTCCCCAGT AGAGGCCAAG TCCCCAGAGA AGGCCAAGTC TCCTGCGAAG 
GAGGAGGCCA AGTCCCCAGA GAAGGCCAAG TCCCCAGAGA AGCCCAAAAG CCCTGTGAAG 
GAGGAGGCCA AGTCCCCAGA GAAGCCCAAA AGCCCTGTGA AGGAGGAGGC CAAGTCCCCA 
GAGAAGCCCA AAAGCCCTGT CAAGGAGGAG GCCAAATCCC CAGAGAAGGC CAAGTCTCCT 
GTGAAGGATG AGGCCAAGGC TCCTGGGAAG GAGGTCCCAA AGAAGGAGGA GGCCAAGTCC 
CCTGTGAAGG AGGAAGAAAA ACCCCAGGAG GTGAGAGCCA AAGAGCCCCC AAAGAAGGCA 
GAGGAAGAGA AAGCTCCAGC CATACCCAAA GCTGAGGAGA AGAAGGACAG CAAGAAAGAT 
GAGGTGCCCA AGAAGGAGGC TCCAAAGCCC GAGGAGAAGA AGGAGCCTGC TGTGGAAAAG 
CCCAAAGAAC CCAAAGCTGA AGCCAAGAAG GAAGAGGCTG AAGATAAGAA AAAAGCAACC 
ACCCCAGAGA AGGAGGCTCC TGCCAAGGGG AAGGAAGAGG CCAAGCCTAA AGAGAAGACT 
GAGGTGGCCA AGAAGGAACC AGATGATGCC AAGCCCAAAG AACCCAGCAA AGCAGCAGAA 
AAGCCAAAGA AGGAAGAGAC GCCGGCAGCA CCGGAGAAAA AAGACGCCAA GGAGGGGAAG 
GCCGCAGAAG CCAAGAAGCC TGAGGAGAAA CCCAAAACAG AGGCCCAAGC CAAGGAACCT 
GGCAAGGAGG CCTCCACACC CGGGAAAGCC AAGGCTGAGA AATCCTCCAG CACAGACCAG 
AAAGACAGCA GACCTGCAGA GAAGGCCGCA GAAGATAAGG CCACCAAGGG AGAGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005218176.1
CDS	79..3198
Translation	MSFSGADALLGAFAPLHGGGSLHYALARKGGAGGARSAAGSSSGFHSWARTSVSSVSASPSRFRGAGTTSSTDSLDTLSNGPEGCVVVARSEKEQLQVLNDRFAGYIDKVRQLEAHNRSLEGEAAALRQQQAGRAAMGELYEREVREMRGAVLRLGAARGQLRLEQEHLLEDIAHVRQRLDDEARQREEAEAATRALARFAQEAEAARVELQKKAQALQEECGYLRRHHQEEVGELLGQIQSSSAAQTQAEARDALKCDVTSALREIRAQLEGHTVQSTLQSEEWFRVRLDRLSEAAKVNTDAMRSAQEEISEYRRQLQARTTELETLKSTKDSLERQRSELEDRHQADIASYQEAIQQLDAELRNTKWEMAAQLREYQDLLNVKMALDIEIAAYRKLLEGEECRIGFGPSPFLLPEGLPKIPSVSTHIKVKSEEKIKMVEKSEKETVIVEEQTEEVQVTEEVTEEEEKEAKEEEGEAVKSPPAEEAASPEKEEAKSPAEAKSPEKAKSPVKEEAKSPQKEEAKSPAEVKSPEKAKSPVEAKSPEKAKSPVKEEAKSPEKAKSPVEAKSPEKAKSPMKEEAKSPEKVKSPVEAKSPEKEEAKSPEKAKSPEKAKSPVKEEAKSPEKAKSPVEAKSPVKEEAKSPEKAKSPVEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVEAKSPEKAKSPAKEEAKSPEKAKSPEKPKSPVKEEAKSPEKPKSPVKEEAKSPEKPKSPVKEEAKSPEKAKSPVKDEAKAPGKEVPKKEEAKSPVKEEEKPQEVRAKEPPKKAEEEKAPAIPKAEEKKDSKKDEVPKKEAPKPEEKKEPAVEKPKEPKAEAKKEEAEDKKKATTPEKEAPAKGKEEAKPKEKTEVAKKEPDDAKPKEPSKAAEKPKKEETPAAPEKKDAKEGKAAEAKKPEEKPKTEAQAKEPGKEASTPGKAKAEKSSSTDQKDSRPAEKAAEDKATKGEK

Target ORF information:

RefSeq Version	XM_005218119.2
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofilament, heavy polypeptide (NEFH), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005218119.2

ORF Insert Sequence:

ATGAGCTTCA GCGGCGCCGA CGCGCTGCTG GGCGCCTTCG CGCCGCTCCA CGGAGGCGGC 
AGCTTGCACT ATGCACTGGC TCGCAAAGGC GGCGCCGGCG GAGCGCGCTC TGCAGCCGGC 
TCCTCCAGCG GATTCCACTC ATGGGCGCGG ACCTCCGTGA GCTCCGTGTC GGCCTCCCCG 
AGCCGCTTCC GAGGCGCCGG GACTACCTCC AGCACCGACT CGCTAGACAC CCTGAGCAAC 
GGACCGGAGG GCTGCGTGGT GGTGGCGCGC AGTGAGAAGG AGCAGCTGCA GGTGCTGAAC 
GACCGCTTCG CGGGCTACAT CGACAAGGTG CGGCAGCTCG AGGCGCATAA CCGCAGCCTG 
GAGGGCGAGG CGGCGGCGCT GCGGCAGCAG CAGGCGGGCC GCGCAGCCAT GGGCGAGCTG 
TACGAGCGCG AGGTGCGCGA GATGCGCGGC GCAGTGCTGC GCTTGGGCGC GGCTCGCGGC 
CAGCTGCGCC TGGAGCAGGA GCACCTGCTG GAGGACATCG CGCACGTGCG CCAGCGCCTG 
GACGACGAGG CCCGGCAGCG CGAGGAGGCC GAGGCAGCGA CGCGCGCACT GGCCCGCTTT 
GCGCAGGAGG CCGAGGCGGC GCGCGTCGAG CTGCAGAAGA AGGCACAAGC CCTGCAGGAA 
GAGTGCGGCT ACCTGCGGCG TCACCACCAG GAGGAGGTGG GCGAGCTGCT CGGCCAGATC 
CAGAGCAGCA GCGCCGCGCA GACGCAGGCC GAGGCGCGCG ATGCCCTCAA GTGCGACGTG 
ACGTCGGCCC TGCGCGAGAT CCGCGCGCAG CTTGAAGGCC ACACGGTGCA GAGCACGCTT 
CAGTCCGAGG AGTGGTTCCG AGTGAGGCTG GACCGACTGT CCGAGGCAGC CAAGGTGAAC 
ACAGACGCCA TGCGCTCAGC ACAGGAGGAG ATATCTGAGT ACCGGCGCCA GCTGCAGGCC 
AGGACCACAG AGCTGGAGAC GCTCAAAAGC ACCAAGGACT CACTGGAGAG GCAGCGCTCT 
GAGCTGGAGG ACCGTCACCA GGCTGACATC GCATCCTACC AGGAGGCCAT CCAGCAGCTG 
GATGCAGAGC TGAGGAACAC CAAGTGGGAG ATGGCAGCCC AGCTCCGAGA GTACCAGGAT 
CTACTCAATG TCAAGATGGC TCTGGACATT GAGATCGCTG CTTACAGAAA ACTCCTGGAG 
GGTGAAGAAT GTCGAATTGG CTTTGGCCCG AGTCCTTTCC TCCTTCCAGA AGGACTCCCC 
AAGATCCCCT CCGTGTCCAC TCACATCAAA GTCAAAAGTG AAGAGAAGAT CAAGATGGTA 
GAAAAGTCAG AGAAGGAAAC TGTGATTGTG GAGGAACAGA CAGAGGAGGT CCAAGTGACT 
GAAGAAGTGA CTGAAGAAGA AGAGAAAGAG GCCAAAGAGG AGGAAGGAGA AGCAGTAAAG 
TCTCCCCCAG CAGAAGAGGC TGCATCCCCA GAGAAGGAGG AGGCCAAGTC ACCAGCCGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAGG AGGCCAAGTC CCCACAGAAG 
GAAGAGGCCA AGTCACCAGC TGAGGTGAAG TCCCCTGAGA AGGCCAAGTC CCCCGTGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAAG AAGCCAAATC CCCAGAGAAG 
GCCAAGTCCC CAGTGGAGGC CAAGTCTCCA GAAAAGGCCA AGTCCCCCAT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGT CAAATCCCCA GTGGAGGCCA AATCCCCAGA GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GAGAAAGCCA AGTCCCCTGT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GTGGAAGCCA AGTCCCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCA GTGGAGGCCA AGTCGCCAGA GAAGGCCAAG 
TCTCCTGTGA AGGAGGAGGC CAAGTCCCCA GAGAAGGCCA AGTCTCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT GTGAAAGAGG AGGCCAAGTC CCCAGAGAAG 
GCCAAGTCTC CTGTGAAGGA GGAGGCCAAG TCGCCAGAGA AGGCCAAGTC TCCTGTGAAA 
GAGGAGGCCA AGTCCCCAGA GAAGGCCAAG TCTCCTGTGA AAGAGGAGGC CAAGTCCCCA 
GAGAAGGCCA AGTCCCCAGT AGAGGCCAAG TCCCCAGAGA AGGCCAAGTC TCCTGCGAAG 
GAGGAGGCCA AGTCCCCAGA GAAGGCCAAG TCCCCAGAGA AGCCCAAAAG CCCTGTGAAG 
GAGGAGGCCA AGTCCCCAGA GAAGCCCAAA AGCCCTGTGA AGGAGGAGGC CAAGTCCCCA 
GAGAAGCCCA AAAGCCCTGT CAAGGAGGAG GCCAAATCCC CAGAGAAGGC CAAGTCTCCT 
GTGAAGGATG AGGCCAAGGC TCCTGGGAAG GAGGTCCCAA AGAAGGAGGA GGCCAAGTCC 
CCTGTGAAGG AGGAAGAAAA ACCCCAGGAG GTGAGAGCCA AAGAGCCCCC AAAGAAGGCA 
GAGGAAGAGA AAGCTCCAGC CATACCCAAA GCTGAGGAGA AGAAGGACAG CAAGAAAGAT 
GAGGTGCCCA AGAAGGAGGC TCCAAAGCCC GAGGAGAAGA AGGAGCCTGC TGTGGAAAAG 
CCCAAAGAAC CCAAAGCTGA AGCCAAGAAG GAAGAGGCTG AAGATAAGAA AAAAGCAACC 
ACCCCAGAGA AGGAGGCTCC TGCCAAGGGG AAGGAAGAGG CCAAGCCTAA AGAGAAGACT 
GAGGTGGCCA AGAAGGAACC AGATGATGCC AAGCCCAAAG AACCCAGCAA AGCAGCAGAA 
AAGCCAAAGA AGGAAGAGAC GCCGGCAGCA CCGGAGAAAA AAGACGCCAA GGAGGGGAAG 
GCCGCAGAAG CCAAGAAGCC TGAGGAGAAA CCCAAAACAG AGGCCCAAGC CAAGGAACCT 
GGCAAGGAGG CCTCCACACC CGGGAAAGCC AAGGCTGAGA AATCCTCCAG CACAGACCAG 
AAAGACAGCA GACCTGCAGA GAAGGCCGCA GAAGATAAGG CCACCAAGGG AGAGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEFH ( XM_010823477.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010821779.2 Bos taurus neurofilament, heavy polypeptide (NEFH), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa28075
Clone ID Related Accession (Same CDS sequence)	XM_005218119.2 , XM_010823477.2
Accession Version	XM_010823477.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3120bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neurofilament heavy polypeptide isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646373.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010823477.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCA GCGGCGCCGA CGCGCTGCTG GGCGCCTTCG CGCCGCTCCA CGGAGGCGGC 
AGCTTGCACT ATGCACTGGC TCGCAAAGGC GGCGCCGGCG GAGCGCGCTC TGCAGCCGGC 
TCCTCCAGCG GATTCCACTC ATGGGCGCGG ACCTCCGTGA GCTCCGTGTC GGCCTCCCCG 
AGCCGCTTCC GAGGCGCCGG GACTACCTCC AGCACCGACT CGCTAGACAC CCTGAGCAAC 
GGACCGGAGG GCTGCGTGGT GGTGGCGCGC AGTGAGAAGG AGCAGCTGCA GGTGCTGAAC 
GACCGCTTCG CGGGCTACAT CGACAAGGTG CGGCAGCTCG AGGCGCATAA CCGCAGCCTG 
GAGGGCGAGG CGGCGGCGCT GCGGCAGCAG CAGGCGGGCC GCGCAGCCAT GGGCGAGCTG 
TACGAGCGCG AGGTGCGCGA GATGCGCGGC GCAGTGCTGC GCTTGGGCGC GGCTCGCGGC 
CAGCTGCGCC TGGAGCAGGA GCACCTGCTG GAGGACATCG CGCACGTGCG CCAGCGCCTG 
GACGACGAGG CCCGGCAGCG CGAGGAGGCC GAGGCAGCGA CGCGCGCACT GGCCCGCTTT 
GCGCAGGAGG CCGAGGCGGC GCGCGTCGAG CTGCAGAAGA AGGCACAAGC CCTGCAGGAA 
GAGTGCGGCT ACCTGCGGCG TCACCACCAG GAGGAGGTGG GCGAGCTGCT CGGCCAGATC 
CAGAGCAGCA GCGCCGCGCA GACGCAGGCC GAGGCGCGCG ATGCCCTCAA GTGCGACGTG 
ACGTCGGCCC TGCGCGAGAT CCGCGCGCAG CTTGAAGGCC ACACGGTGCA GAGCACGCTT 
CAGTCCGAGG AGTGGTTCCG AGTGAGGCTG GACCGACTGT CCGAGGCAGC CAAGGTGAAC 
ACAGACGCCA TGCGCTCAGC ACAGGAGGAG ATATCTGAGT ACCGGCGCCA GCTGCAGGCC 
AGGACCACAG AGCTGGAGAC GCTCAAAAGC ACCAAGGACT CACTGGAGAG GCAGCGCTCT 
GAGCTGGAGG ACCGTCACCA GGCTGACATC GCATCCTACC AGGAGGCCAT CCAGCAGCTG 
GATGCAGAGC TGAGGAACAC CAAGTGGGAG ATGGCAGCCC AGCTCCGAGA GTACCAGGAT 
CTACTCAATG TCAAGATGGC TCTGGACATT GAGATCGCTG CTTACAGAAA ACTCCTGGAG 
GGTGAAGAAT GTCGAATTGG CTTTGGCCCG AGTCCTTTCC TCCTTCCAGA AGGACTCCCC 
AAGATCCCCT CCGTGTCCAC TCACATCAAA GTCAAAAGTG AAGAGAAGAT CAAGATGGTA 
GAAAAGTCAG AGAAGGAAAC TGTGATTGTG GAGGAACAGA CAGAGGAGGT CCAAGTGACT 
GAAGAAGTGA CTGAAGAAGA AGAGAAAGAG GCCAAAGAGG AGGAAGGAGA AGCAGTAAAG 
TCTCCCCCAG CAGAAGAGGC TGCATCCCCA GAGAAGGAGG AGGCCAAGTC ACCAGCCGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAGG AGGCCAAGTC CCCACAGAAG 
GAAGAGGCCA AGTCACCAGC TGAGGTGAAG TCCCCTGAGA AGGCCAAGTC CCCCGTGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAAG AAGCCAAATC CCCAGAGAAG 
GCCAAGTCCC CAGTGGAGGC CAAGTCTCCA GAAAAGGCCA AGTCCCCCAT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGT CAAATCCCCA GTGGAGGCCA AATCCCCAGA GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GAGAAAGCCA AGTCCCCTGT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GTGGAAGCCA AGTCCCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCA GTGGAGGCCA AGTCGCCAGA GAAGGCCAAG 
TCTCCTGTGA AGGAGGAGGC CAAGTCCCCA GAGAAGGCCA AGTCTCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT GTGAAAGAGG AGGCCAAGTC CCCAGAGAAG 
GCCAAGTCTC CTGTGAAGGA GGAGGCCAAG TCGCCAGAGA AGGCCAAGTC TCCTGTGAAA 
GAGGAGGCCA AGTCCCCAGA GAAGGCCAAG TCTCCTGTGA AAGAGGAGGC CAAGTCCCCA 
GAGAAGGCCA AGTCCCCAGT AGAGGCCAAG TCCCCAGAGA AGGCCAAGTC TCCTGCGAAG 
GAGGAGGCCA AGTCCCCAGA GAAGGCCAAG TCCCCAGAGA AGCCCAAAAG CCCTGTGAAG 
GAGGAGGCCA AGTCCCCAGA GAAGCCCAAA AGCCCTGTGA AGGAGGAGGC CAAGTCCCCA 
GAGAAGCCCA AAAGCCCTGT CAAGGAGGAG GCCAAATCCC CAGAGAAGGC CAAGTCTCCT 
GTGAAGGATG AGGCCAAGGC TCCTGGGAAG GAGGTCCCAA AGAAGGAGGA GGCCAAGTCC 
CCTGTGAAGG AGGAAGAAAA ACCCCAGGAG GTGAGAGCCA AAGAGCCCCC AAAGAAGGCA 
GAGGAAGAGA AAGCTCCAGC CATACCCAAA GCTGAGGAGA AGAAGGACAG CAAGAAAGAT 
GAGGTGCCCA AGAAGGAGGC TCCAAAGCCC GAGGAGAAGA AGGAGCCTGC TGTGGAAAAG 
CCCAAAGAAC CCAAAGCTGA AGCCAAGAAG GAAGAGGCTG AAGATAAGAA AAAAGCAACC 
ACCCCAGAGA AGGAGGCTCC TGCCAAGGGG AAGGAAGAGG CCAAGCCTAA AGAGAAGACT 
GAGGTGGCCA AGAAGGAACC AGATGATGCC AAGCCCAAAG AACCCAGCAA AGCAGCAGAA 
AAGCCAAAGA AGGAAGAGAC GCCGGCAGCA CCGGAGAAAA AAGACGCCAA GGAGGGGAAG 
GCCGCAGAAG CCAAGAAGCC TGAGGAGAAA CCCAAAACAG AGGCCCAAGC CAAGGAACCT 
GGCAAGGAGG CCTCCACACC CGGGAAAGCC AAGGCTGAGA AATCCTCCAG CACAGACCAG 
AAAGACAGCA GACCTGCAGA GAAGGCCGCA GAAGATAAGG CCACCAAGGG AGAGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010821779.2
CDS	79..3198
Translation	MSFSGADALLGAFAPLHGGGSLHYALARKGGAGGARSAAGSSSGFHSWARTSVSSVSASPSRFRGAGTTSSTDSLDTLSNGPEGCVVVARSEKEQLQVLNDRFAGYIDKVRQLEAHNRSLEGEAAALRQQQAGRAAMGELYEREVREMRGAVLRLGAARGQLRLEQEHLLEDIAHVRQRLDDEARQREEAEAATRALARFAQEAEAARVELQKKAQALQEECGYLRRHHQEEVGELLGQIQSSSAAQTQAEARDALKCDVTSALREIRAQLEGHTVQSTLQSEEWFRVRLDRLSEAAKVNTDAMRSAQEEISEYRRQLQARTTELETLKSTKDSLERQRSELEDRHQADIASYQEAIQQLDAELRNTKWEMAAQLREYQDLLNVKMALDIEIAAYRKLLEGEECRIGFGPSPFLLPEGLPKIPSVSTHIKVKSEEKIKMVEKSEKETVIVEEQTEEVQVTEEVTEEEEKEAKEEEGEAVKSPPAEEAASPEKEEAKSPAEAKSPEKAKSPVKEEAKSPQKEEAKSPAEVKSPEKAKSPVEAKSPEKAKSPVKEEAKSPEKAKSPVEAKSPEKAKSPMKEEAKSPEKVKSPVEAKSPEKEEAKSPEKAKSPEKAKSPVKEEAKSPEKAKSPVEAKSPVKEEAKSPEKAKSPVEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVEAKSPEKAKSPAKEEAKSPEKAKSPEKPKSPVKEEAKSPEKPKSPVKEEAKSPEKPKSPVKEEAKSPEKAKSPVKDEAKAPGKEVPKKEEAKSPVKEEEKPQEVRAKEPPKKAEEEKAPAIPKAEEKKDSKKDEVPKKEAPKPEEKKEPAVEKPKEPKAEAKKEEAEDKKKATTPEKEAPAKGKEEAKPKEKTEVAKKEPDDAKPKEPSKAAEKPKKEETPAAPEKKDAKEGKAAEAKKPEEKPKTEAQAKEPGKEASTPGKAKAEKSSSTDQKDSRPAEKAAEDKATKGEK

Target ORF information:

RefSeq Version	XM_010823477.2
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofilament, heavy polypeptide (NEFH), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010823477.2

ORF Insert Sequence:

ATGAGCTTCA GCGGCGCCGA CGCGCTGCTG GGCGCCTTCG CGCCGCTCCA CGGAGGCGGC 
AGCTTGCACT ATGCACTGGC TCGCAAAGGC GGCGCCGGCG GAGCGCGCTC TGCAGCCGGC 
TCCTCCAGCG GATTCCACTC ATGGGCGCGG ACCTCCGTGA GCTCCGTGTC GGCCTCCCCG 
AGCCGCTTCC GAGGCGCCGG GACTACCTCC AGCACCGACT CGCTAGACAC CCTGAGCAAC 
GGACCGGAGG GCTGCGTGGT GGTGGCGCGC AGTGAGAAGG AGCAGCTGCA GGTGCTGAAC 
GACCGCTTCG CGGGCTACAT CGACAAGGTG CGGCAGCTCG AGGCGCATAA CCGCAGCCTG 
GAGGGCGAGG CGGCGGCGCT GCGGCAGCAG CAGGCGGGCC GCGCAGCCAT GGGCGAGCTG 
TACGAGCGCG AGGTGCGCGA GATGCGCGGC GCAGTGCTGC GCTTGGGCGC GGCTCGCGGC 
CAGCTGCGCC TGGAGCAGGA GCACCTGCTG GAGGACATCG CGCACGTGCG CCAGCGCCTG 
GACGACGAGG CCCGGCAGCG CGAGGAGGCC GAGGCAGCGA CGCGCGCACT GGCCCGCTTT 
GCGCAGGAGG CCGAGGCGGC GCGCGTCGAG CTGCAGAAGA AGGCACAAGC CCTGCAGGAA 
GAGTGCGGCT ACCTGCGGCG TCACCACCAG GAGGAGGTGG GCGAGCTGCT CGGCCAGATC 
CAGAGCAGCA GCGCCGCGCA GACGCAGGCC GAGGCGCGCG ATGCCCTCAA GTGCGACGTG 
ACGTCGGCCC TGCGCGAGAT CCGCGCGCAG CTTGAAGGCC ACACGGTGCA GAGCACGCTT 
CAGTCCGAGG AGTGGTTCCG AGTGAGGCTG GACCGACTGT CCGAGGCAGC CAAGGTGAAC 
ACAGACGCCA TGCGCTCAGC ACAGGAGGAG ATATCTGAGT ACCGGCGCCA GCTGCAGGCC 
AGGACCACAG AGCTGGAGAC GCTCAAAAGC ACCAAGGACT CACTGGAGAG GCAGCGCTCT 
GAGCTGGAGG ACCGTCACCA GGCTGACATC GCATCCTACC AGGAGGCCAT CCAGCAGCTG 
GATGCAGAGC TGAGGAACAC CAAGTGGGAG ATGGCAGCCC AGCTCCGAGA GTACCAGGAT 
CTACTCAATG TCAAGATGGC TCTGGACATT GAGATCGCTG CTTACAGAAA ACTCCTGGAG 
GGTGAAGAAT GTCGAATTGG CTTTGGCCCG AGTCCTTTCC TCCTTCCAGA AGGACTCCCC 
AAGATCCCCT CCGTGTCCAC TCACATCAAA GTCAAAAGTG AAGAGAAGAT CAAGATGGTA 
GAAAAGTCAG AGAAGGAAAC TGTGATTGTG GAGGAACAGA CAGAGGAGGT CCAAGTGACT 
GAAGAAGTGA CTGAAGAAGA AGAGAAAGAG GCCAAAGAGG AGGAAGGAGA AGCAGTAAAG 
TCTCCCCCAG CAGAAGAGGC TGCATCCCCA GAGAAGGAGG AGGCCAAGTC ACCAGCCGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAGG AGGCCAAGTC CCCACAGAAG 
GAAGAGGCCA AGTCACCAGC TGAGGTGAAG TCCCCTGAGA AGGCCAAGTC CCCCGTGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAAG AAGCCAAATC CCCAGAGAAG 
GCCAAGTCCC CAGTGGAGGC CAAGTCTCCA GAAAAGGCCA AGTCCCCCAT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGT CAAATCCCCA GTGGAGGCCA AATCCCCAGA GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GAGAAAGCCA AGTCCCCTGT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GTGGAAGCCA AGTCCCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCA GTGGAGGCCA AGTCGCCAGA GAAGGCCAAG 
TCTCCTGTGA AGGAGGAGGC CAAGTCCCCA GAGAAGGCCA AGTCTCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT GTGAAAGAGG AGGCCAAGTC CCCAGAGAAG 
GCCAAGTCTC CTGTGAAGGA GGAGGCCAAG TCGCCAGAGA AGGCCAAGTC TCCTGTGAAA 
GAGGAGGCCA AGTCCCCAGA GAAGGCCAAG TCTCCTGTGA AAGAGGAGGC CAAGTCCCCA 
GAGAAGGCCA AGTCCCCAGT AGAGGCCAAG TCCCCAGAGA AGGCCAAGTC TCCTGCGAAG 
GAGGAGGCCA AGTCCCCAGA GAAGGCCAAG TCCCCAGAGA AGCCCAAAAG CCCTGTGAAG 
GAGGAGGCCA AGTCCCCAGA GAAGCCCAAA AGCCCTGTGA AGGAGGAGGC CAAGTCCCCA 
GAGAAGCCCA AAAGCCCTGT CAAGGAGGAG GCCAAATCCC CAGAGAAGGC CAAGTCTCCT 
GTGAAGGATG AGGCCAAGGC TCCTGGGAAG GAGGTCCCAA AGAAGGAGGA GGCCAAGTCC 
CCTGTGAAGG AGGAAGAAAA ACCCCAGGAG GTGAGAGCCA AAGAGCCCCC AAAGAAGGCA 
GAGGAAGAGA AAGCTCCAGC CATACCCAAA GCTGAGGAGA AGAAGGACAG CAAGAAAGAT 
GAGGTGCCCA AGAAGGAGGC TCCAAAGCCC GAGGAGAAGA AGGAGCCTGC TGTGGAAAAG 
CCCAAAGAAC CCAAAGCTGA AGCCAAGAAG GAAGAGGCTG AAGATAAGAA AAAAGCAACC 
ACCCCAGAGA AGGAGGCTCC TGCCAAGGGG AAGGAAGAGG CCAAGCCTAA AGAGAAGACT 
GAGGTGGCCA AGAAGGAACC AGATGATGCC AAGCCCAAAG AACCCAGCAA AGCAGCAGAA 
AAGCCAAAGA AGGAAGAGAC GCCGGCAGCA CCGGAGAAAA AAGACGCCAA GGAGGGGAAG 
GCCGCAGAAG CCAAGAAGCC TGAGGAGAAA CCCAAAACAG AGGCCCAAGC CAAGGAACCT 
GGCAAGGAGG CCTCCACACC CGGGAAAGCC AAGGCTGAGA AATCCTCCAG CACAGACCAG 
AAAGACAGCA GACCTGCAGA GAAGGCCGCA GAAGATAAGG CCACCAAGGG AGAGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEFH ( XM_005218121.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005218178.1 Bos taurus neurofilament heavy (NEFH), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa28077
Clone ID Related Accession (Same CDS sequence)	XM_005218121.3 , XM_010823479.2 , XM_005218121.2
Accession Version	XM_005218121.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3084bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	neurofilament heavy polypeptide isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005218121.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCA GCGGCGCCGA CGCGCTGCTG GGCGCCTTCG CGCCGCTCCA CGGAGGCGGC 
AGCTTGCACT ATGCACTGGC TCGCAAAGGC GGCGCCGGCG GAGCGCGCTC TGCAGCCGGC 
TCCTCCAGCG GATTCCACTC ATGGGCGCGG ACCTCCGTGA GCTCCGTGTC GGCCTCCCCG 
AGCCGCTTCC GAGGCGCCGG GACTACCTCC AGCACCGACT CGCTAGACAC CCTGAGCAAC 
GGACCGGAGG GCTGCGTGGT GGTGGCGCGC AGTGAGAAGG AGCAGCTGCA GGTGCTGAAC 
GACCGCTTCG CGGGCTACAT CGACAAGGTG CGGCAGCTCG AGGCGCATAA CCGCAGCCTG 
GAGGGCGAGG CGGCGGCGCT GCGGCAGCAG CAGGCGGGCC GCGCAGCCAT GGGCGAGCTG 
TACGAGCGCG AGGTGCGCGA GATGCGCGGC GCAGTGCTGC GCTTGGGCGC GGCTCGCGGC 
CAGCTGCGCC TGGAGCAGGA GCACCTGCTG GAGGACATCG CGCACGTGCG CCAGCGCCTG 
GACGACGAGG CCCGGCAGCG CGAGGAGGCC GAGGCAGCGA CGCGCGCACT GGCCCGCTTT 
GCGCAGGAGG CCGAGGCGGC GCGCGTCGAG CTGCAGAAGA AGGCACAAGC CCTGCAGGAA 
GAGTGCGGCT ACCTGCGGCG TCACCACCAG GAGGAGGTGG GCGAGCTGCT CGGCCAGATC 
CAGAGCAGCA GCGCCGCGCA GACGCAGGCC GAGGCGCGCG ATGCCCTCAA GTGCGACGTG 
ACGTCGGCCC TGCGCGAGAT CCGCGCGCAG CTTGAAGGCC ACACGGTGCA GAGCACGCTT 
CAGTCCGAGG AGTGGTTCCG AGTGAGGCTG GACCGACTGT CCGAGGCAGC CAAGGTGAAC 
ACAGACGCCA TGCGCTCAGC ACAGGAGGAG ATATCTGAGT ACCGGCGCCA GCTGCAGGCC 
AGGACCACAG AGCTGGAGAC GCTCAAAAGC ACCAAGGACT CACTGGAGAG GCAGCGCTCT 
GAGCTGGAGG ACCGTCACCA GGCTGACATC GCATCCTACC AGGAGGCCAT CCAGCAGCTG 
GATGCAGAGC TGAGGAACAC CAAGTGGGAG ATGGCAGCCC AGCTCCGAGA GTACCAGGAT 
CTACTCAATG TCAAGATGGC TCTGGACATT GAGATCGCTG CTTACAGAAA ACTCCTGGAG 
GGTGAAGAAT GTCGAATTGG CTTTGGCCCG AGTCCTTTCC TCCTTCCAGA AGGACTCCCC 
AAGATCCCCT CCGTGTCCAC TCACATCAAA GTCAAAAGTG AAGAGAAGAT CAAGATGGTA 
GAAAAGTCAG AGAAGGAAAC TGTGATTGTG GAGGAACAGA CAGAGGAGGT CCAAGTGACT 
GAAGAAGTGA CTGAAGAAGA AGAGAAAGAG GCCAAAGAGG AGGAAGGAGA AGCAGTAAAG 
TCTCCCCCAG CAGAAGAGGC TGCATCCCCA GAGAAGGAGG AGGCCAAGTC ACCAGCCGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAGG AGGCCAAGTC CCCACAGAAG 
GAAGAGGCCA AGTCACCAGC TGAGGTGAAG TCCCCTGAGA AGGCCAAGTC CCCCGTGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAAG AAGCCAAATC CCCAGAGAAG 
GCCAAGTCCC CAGTGGAGGC CAAGTCTCCA GAAAAGGCCA AGTCCCCCAT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGT CAAATCCCCA GTGGAGGCCA AATCCCCAGA GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GAGAAAGCCA AGTCCCCTGT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GTGGAAGCCA AGTCCCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCA GTGGAGGCCA AGTCGCCAGA GAAGGCCAAG 
TCTCCTGTGA AGGAGGAGGC CAAGTCCCCA GAGAAGGCCA AGTCTCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT GTGAAAGAGG AGGCCAAGTC CCCAGAGAAG 
GCCAAGTCTC CTGCGAAGGA GGAGGCCAAG TCGCCAGAGA AGGCCAAGTC TCCTGTGAAA 
GAGGAGGCCA AGTCCCCAGA GAAGGCCAAG TCTCCTGTGA AAGAGGAGGC CAAGTCCCCA 
GAGAAGGCCA AGTCTCCTGC GAAGGAGGAG GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA 
GAGAAGCCCA AAAGCCCTGT GAAGGAGGAG GCCAAGTCCC CAGAGAAGCC CAAAAGCCCT 
GTGAAGGAGG AGGCCAAGTC CCCAGAGAAG CCCAAAAGCC CTGTCAAGGA GGAGGCCAAA 
TCCCCAGAGA AGGCCAAGTC TCCTGTGAAG GATGAGGCCA AGGCTCCTGG GAAGGAGGTC 
CCAAAGAAGG AGGAGGCCAA GTCCCCTGTG AAGGAGGAAG AAAAACCCCA GGAGGTGAGA 
GCCAAAGAGC CCCCAAAGAA GGCAGAGGAA GAGAAAGCTC CAGCCATACC CAAAGCTGAG 
GAGAAGAAGG ACAGCAAGAA AGATGAGGTG CCCAAGAAGG AGGCTCCAAA GCCCGAGGAG 
AAGAAGGAGC CTGCTGTGGA AAAGCCCAAA GAACCCAAAG CTGAAGCCAA GAAGGAAGAG 
GCTGAAGATA AGAAAAAAGC AACCACCCCA GAGAAGGAGG CTCCTGCCAA GGGGAAGGAA 
GAGGCCAAGC CTAAAGAGAA GACTGAGGTG GCCAAGAAGG AACCAGATGA TGCCAAGCCC 
AAAGAACCCA GCAAAGCAGC AGAAAAGCCA AAGAAGGAAG AGACGCCGGC AGCACCGGAG 
AAAAAAGACG CCAAGGAGGG GAAGGCCGCA GAAGCCAAGA AGCCTGAGGA GAAACCCAAA 
ACAGAGGCCC AAGCCAAGGA ACCTGGCAAG GAGGCCTCCA CACCCGGGAA AGCCAAGGCT 
GAGAAATCCT CCAGCACAGA CCAGAAAGAC AGCAGACCTG CAGAGAAGGC CGCAGAAGAT 
AAGGCCACCA AGGGAGAGAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005218178.1
CDS	76..3159
Translation	MSFSGADALLGAFAPLHGGGSLHYALARKGGAGGARSAAGSSSGFHSWARTSVSSVSASPSRFRGAGTTSSTDSLDTLSNGPEGCVVVARSEKEQLQVLNDRFAGYIDKVRQLEAHNRSLEGEAAALRQQQAGRAAMGELYEREVREMRGAVLRLGAARGQLRLEQEHLLEDIAHVRQRLDDEARQREEAEAATRALARFAQEAEAARVELQKKAQALQEECGYLRRHHQEEVGELLGQIQSSSAAQTQAEARDALKCDVTSALREIRAQLEGHTVQSTLQSEEWFRVRLDRLSEAAKVNTDAMRSAQEEISEYRRQLQARTTELETLKSTKDSLERQRSELEDRHQADIASYQEAIQQLDAELRNTKWEMAAQLREYQDLLNVKMALDIEIAAYRKLLEGEECRIGFGPSPFLLPEGLPKIPSVSTHIKVKSEEKIKMVEKSEKETVIVEEQTEEVQVTEEVTEEEEKEAKEEEGEAVKSPPAEEAASPEKEEAKSPAEAKSPEKAKSPVKEEAKSPQKEEAKSPAEVKSPEKAKSPVEAKSPEKAKSPVKEEAKSPEKAKSPVEAKSPEKAKSPMKEEAKSPEKVKSPVEAKSPEKEEAKSPEKAKSPEKAKSPVKEEAKSPEKAKSPVEAKSPVKEEAKSPEKAKSPVEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPAKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPAKEEAKSPEKAKSPEKPKSPVKEEAKSPEKPKSPVKEEAKSPEKPKSPVKEEAKSPEKAKSPVKDEAKAPGKEVPKKEEAKSPVKEEEKPQEVRAKEPPKKAEEEKAPAIPKAEEKKDSKKDEVPKKEAPKPEEKKEPAVEKPKEPKAEAKKEEAEDKKKATTPEKEAPAKGKEEAKPKEKTEVAKKEPDDAKPKEPSKAAEKPKKEETPAAPEKKDAKEGKAAEAKKPEEKPKTEAQAKEPGKEASTPGKAKAEKSSSTDQKDSRPAEKAAEDKATKGEK

Target ORF information:

RefSeq Version	XM_005218121.3
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofilament heavy (NEFH), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005218121.3

ORF Insert Sequence:

ATGAGCTTCA GCGGCGCCGA CGCGCTGCTG GGCGCCTTCG CGCCGCTCCA CGGAGGCGGC 
AGCTTGCACT ATGCACTGGC TCGCAAAGGC GGCGCCGGCG GAGCGCGCTC TGCAGCCGGC 
TCCTCCAGCG GATTCCACTC ATGGGCGCGG ACCTCCGTGA GCTCCGTGTC GGCCTCCCCG 
AGCCGCTTCC GAGGCGCCGG GACTACCTCC AGCACCGACT CGCTAGACAC CCTGAGCAAC 
GGACCGGAGG GCTGCGTGGT GGTGGCGCGC AGTGAGAAGG AGCAGCTGCA GGTGCTGAAC 
GACCGCTTCG CGGGCTACAT CGACAAGGTG CGGCAGCTCG AGGCGCATAA CCGCAGCCTG 
GAGGGCGAGG CGGCGGCGCT GCGGCAGCAG CAGGCGGGCC GCGCAGCCAT GGGCGAGCTG 
TACGAGCGCG AGGTGCGCGA GATGCGCGGC GCAGTGCTGC GCTTGGGCGC GGCTCGCGGC 
CAGCTGCGCC TGGAGCAGGA GCACCTGCTG GAGGACATCG CGCACGTGCG CCAGCGCCTG 
GACGACGAGG CCCGGCAGCG CGAGGAGGCC GAGGCAGCGA CGCGCGCACT GGCCCGCTTT 
GCGCAGGAGG CCGAGGCGGC GCGCGTCGAG CTGCAGAAGA AGGCACAAGC CCTGCAGGAA 
GAGTGCGGCT ACCTGCGGCG TCACCACCAG GAGGAGGTGG GCGAGCTGCT CGGCCAGATC 
CAGAGCAGCA GCGCCGCGCA GACGCAGGCC GAGGCGCGCG ATGCCCTCAA GTGCGACGTG 
ACGTCGGCCC TGCGCGAGAT CCGCGCGCAG CTTGAAGGCC ACACGGTGCA GAGCACGCTT 
CAGTCCGAGG AGTGGTTCCG AGTGAGGCTG GACCGACTGT CCGAGGCAGC CAAGGTGAAC 
ACAGACGCCA TGCGCTCAGC ACAGGAGGAG ATATCTGAGT ACCGGCGCCA GCTGCAGGCC 
AGGACCACAG AGCTGGAGAC GCTCAAAAGC ACCAAGGACT CACTGGAGAG GCAGCGCTCT 
GAGCTGGAGG ACCGTCACCA GGCTGACATC GCATCCTACC AGGAGGCCAT CCAGCAGCTG 
GATGCAGAGC TGAGGAACAC CAAGTGGGAG ATGGCAGCCC AGCTCCGAGA GTACCAGGAT 
CTACTCAATG TCAAGATGGC TCTGGACATT GAGATCGCTG CTTACAGAAA ACTCCTGGAG 
GGTGAAGAAT GTCGAATTGG CTTTGGCCCG AGTCCTTTCC TCCTTCCAGA AGGACTCCCC 
AAGATCCCCT CCGTGTCCAC TCACATCAAA GTCAAAAGTG AAGAGAAGAT CAAGATGGTA 
GAAAAGTCAG AGAAGGAAAC TGTGATTGTG GAGGAACAGA CAGAGGAGGT CCAAGTGACT 
GAAGAAGTGA CTGAAGAAGA AGAGAAAGAG GCCAAAGAGG AGGAAGGAGA AGCAGTAAAG 
TCTCCCCCAG CAGAAGAGGC TGCATCCCCA GAGAAGGAGG AGGCCAAGTC ACCAGCCGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAGG AGGCCAAGTC CCCACAGAAG 
GAAGAGGCCA AGTCACCAGC TGAGGTGAAG TCCCCTGAGA AGGCCAAGTC CCCCGTGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAAG AAGCCAAATC CCCAGAGAAG 
GCCAAGTCCC CAGTGGAGGC CAAGTCTCCA GAAAAGGCCA AGTCCCCCAT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGT CAAATCCCCA GTGGAGGCCA AATCCCCAGA GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GAGAAAGCCA AGTCCCCTGT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GTGGAAGCCA AGTCCCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCA GTGGAGGCCA AGTCGCCAGA GAAGGCCAAG 
TCTCCTGTGA AGGAGGAGGC CAAGTCCCCA GAGAAGGCCA AGTCTCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT GTGAAAGAGG AGGCCAAGTC CCCAGAGAAG 
GCCAAGTCTC CTGCGAAGGA GGAGGCCAAG TCGCCAGAGA AGGCCAAGTC TCCTGTGAAA 
GAGGAGGCCA AGTCCCCAGA GAAGGCCAAG TCTCCTGTGA AAGAGGAGGC CAAGTCCCCA 
GAGAAGGCCA AGTCTCCTGC GAAGGAGGAG GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA 
GAGAAGCCCA AAAGCCCTGT GAAGGAGGAG GCCAAGTCCC CAGAGAAGCC CAAAAGCCCT 
GTGAAGGAGG AGGCCAAGTC CCCAGAGAAG CCCAAAAGCC CTGTCAAGGA GGAGGCCAAA 
TCCCCAGAGA AGGCCAAGTC TCCTGTGAAG GATGAGGCCA AGGCTCCTGG GAAGGAGGTC 
CCAAAGAAGG AGGAGGCCAA GTCCCCTGTG AAGGAGGAAG AAAAACCCCA GGAGGTGAGA 
GCCAAAGAGC CCCCAAAGAA GGCAGAGGAA GAGAAAGCTC CAGCCATACC CAAAGCTGAG 
GAGAAGAAGG ACAGCAAGAA AGATGAGGTG CCCAAGAAGG AGGCTCCAAA GCCCGAGGAG 
AAGAAGGAGC CTGCTGTGGA AAAGCCCAAA GAACCCAAAG CTGAAGCCAA GAAGGAAGAG 
GCTGAAGATA AGAAAAAAGC AACCACCCCA GAGAAGGAGG CTCCTGCCAA GGGGAAGGAA 
GAGGCCAAGC CTAAAGAGAA GACTGAGGTG GCCAAGAAGG AACCAGATGA TGCCAAGCCC 
AAAGAACCCA GCAAAGCAGC AGAAAAGCCA AAGAAGGAAG AGACGCCGGC AGCACCGGAG 
AAAAAAGACG CCAAGGAGGG GAAGGCCGCA GAAGCCAAGA AGCCTGAGGA GAAACCCAAA 
ACAGAGGCCC AAGCCAAGGA ACCTGGCAAG GAGGCCTCCA CACCCGGGAA AGCCAAGGCT 
GAGAAATCCT CCAGCACAGA CCAGAAAGAC AGCAGACCTG CAGAGAAGGC CGCAGAAGAT 
AAGGCCACCA AGGGAGAGAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEFH ( XM_010823479.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010821781.2 Bos taurus neurofilament, heavy polypeptide (NEFH), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa28077
Clone ID Related Accession (Same CDS sequence)	XM_005218121.3 , XM_010823479.2 , XM_005218121.2
Accession Version	XM_010823479.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3084bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neurofilament heavy polypeptide isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646373.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010823479.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCA GCGGCGCCGA CGCGCTGCTG GGCGCCTTCG CGCCGCTCCA CGGAGGCGGC 
AGCTTGCACT ATGCACTGGC TCGCAAAGGC GGCGCCGGCG GAGCGCGCTC TGCAGCCGGC 
TCCTCCAGCG GATTCCACTC ATGGGCGCGG ACCTCCGTGA GCTCCGTGTC GGCCTCCCCG 
AGCCGCTTCC GAGGCGCCGG GACTACCTCC AGCACCGACT CGCTAGACAC CCTGAGCAAC 
GGACCGGAGG GCTGCGTGGT GGTGGCGCGC AGTGAGAAGG AGCAGCTGCA GGTGCTGAAC 
GACCGCTTCG CGGGCTACAT CGACAAGGTG CGGCAGCTCG AGGCGCATAA CCGCAGCCTG 
GAGGGCGAGG CGGCGGCGCT GCGGCAGCAG CAGGCGGGCC GCGCAGCCAT GGGCGAGCTG 
TACGAGCGCG AGGTGCGCGA GATGCGCGGC GCAGTGCTGC GCTTGGGCGC GGCTCGCGGC 
CAGCTGCGCC TGGAGCAGGA GCACCTGCTG GAGGACATCG CGCACGTGCG CCAGCGCCTG 
GACGACGAGG CCCGGCAGCG CGAGGAGGCC GAGGCAGCGA CGCGCGCACT GGCCCGCTTT 
GCGCAGGAGG CCGAGGCGGC GCGCGTCGAG CTGCAGAAGA AGGCACAAGC CCTGCAGGAA 
GAGTGCGGCT ACCTGCGGCG TCACCACCAG GAGGAGGTGG GCGAGCTGCT CGGCCAGATC 
CAGAGCAGCA GCGCCGCGCA GACGCAGGCC GAGGCGCGCG ATGCCCTCAA GTGCGACGTG 
ACGTCGGCCC TGCGCGAGAT CCGCGCGCAG CTTGAAGGCC ACACGGTGCA GAGCACGCTT 
CAGTCCGAGG AGTGGTTCCG AGTGAGGCTG GACCGACTGT CCGAGGCAGC CAAGGTGAAC 
ACAGACGCCA TGCGCTCAGC ACAGGAGGAG ATATCTGAGT ACCGGCGCCA GCTGCAGGCC 
AGGACCACAG AGCTGGAGAC GCTCAAAAGC ACCAAGGACT CACTGGAGAG GCAGCGCTCT 
GAGCTGGAGG ACCGTCACCA GGCTGACATC GCATCCTACC AGGAGGCCAT CCAGCAGCTG 
GATGCAGAGC TGAGGAACAC CAAGTGGGAG ATGGCAGCCC AGCTCCGAGA GTACCAGGAT 
CTACTCAATG TCAAGATGGC TCTGGACATT GAGATCGCTG CTTACAGAAA ACTCCTGGAG 
GGTGAAGAAT GTCGAATTGG CTTTGGCCCG AGTCCTTTCC TCCTTCCAGA AGGACTCCCC 
AAGATCCCCT CCGTGTCCAC TCACATCAAA GTCAAAAGTG AAGAGAAGAT CAAGATGGTA 
GAAAAGTCAG AGAAGGAAAC TGTGATTGTG GAGGAACAGA CAGAGGAGGT CCAAGTGACT 
GAAGAAGTGA CTGAAGAAGA AGAGAAAGAG GCCAAAGAGG AGGAAGGAGA AGCAGTAAAG 
TCTCCCCCAG CAGAAGAGGC TGCATCCCCA GAGAAGGAGG AGGCCAAGTC ACCAGCCGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAGG AGGCCAAGTC CCCACAGAAG 
GAAGAGGCCA AGTCACCAGC TGAGGTGAAG TCCCCTGAGA AGGCCAAGTC CCCCGTGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAAG AAGCCAAATC CCCAGAGAAG 
GCCAAGTCCC CAGTGGAGGC CAAGTCTCCA GAAAAGGCCA AGTCCCCCAT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGT CAAATCCCCA GTGGAGGCCA AATCCCCAGA GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GAGAAAGCCA AGTCCCCTGT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GTGGAAGCCA AGTCCCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCA GTGGAGGCCA AGTCGCCAGA GAAGGCCAAG 
TCTCCTGTGA AGGAGGAGGC CAAGTCCCCA GAGAAGGCCA AGTCTCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT GTGAAAGAGG AGGCCAAGTC CCCAGAGAAG 
GCCAAGTCTC CTGCGAAGGA GGAGGCCAAG TCGCCAGAGA AGGCCAAGTC TCCTGTGAAA 
GAGGAGGCCA AGTCCCCAGA GAAGGCCAAG TCTCCTGTGA AAGAGGAGGC CAAGTCCCCA 
GAGAAGGCCA AGTCTCCTGC GAAGGAGGAG GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA 
GAGAAGCCCA AAAGCCCTGT GAAGGAGGAG GCCAAGTCCC CAGAGAAGCC CAAAAGCCCT 
GTGAAGGAGG AGGCCAAGTC CCCAGAGAAG CCCAAAAGCC CTGTCAAGGA GGAGGCCAAA 
TCCCCAGAGA AGGCCAAGTC TCCTGTGAAG GATGAGGCCA AGGCTCCTGG GAAGGAGGTC 
CCAAAGAAGG AGGAGGCCAA GTCCCCTGTG AAGGAGGAAG AAAAACCCCA GGAGGTGAGA 
GCCAAAGAGC CCCCAAAGAA GGCAGAGGAA GAGAAAGCTC CAGCCATACC CAAAGCTGAG 
GAGAAGAAGG ACAGCAAGAA AGATGAGGTG CCCAAGAAGG AGGCTCCAAA GCCCGAGGAG 
AAGAAGGAGC CTGCTGTGGA AAAGCCCAAA GAACCCAAAG CTGAAGCCAA GAAGGAAGAG 
GCTGAAGATA AGAAAAAAGC AACCACCCCA GAGAAGGAGG CTCCTGCCAA GGGGAAGGAA 
GAGGCCAAGC CTAAAGAGAA GACTGAGGTG GCCAAGAAGG AACCAGATGA TGCCAAGCCC 
AAAGAACCCA GCAAAGCAGC AGAAAAGCCA AAGAAGGAAG AGACGCCGGC AGCACCGGAG 
AAAAAAGACG CCAAGGAGGG GAAGGCCGCA GAAGCCAAGA AGCCTGAGGA GAAACCCAAA 
ACAGAGGCCC AAGCCAAGGA ACCTGGCAAG GAGGCCTCCA CACCCGGGAA AGCCAAGGCT 
GAGAAATCCT CCAGCACAGA CCAGAAAGAC AGCAGACCTG CAGAGAAGGC CGCAGAAGAT 
AAGGCCACCA AGGGAGAGAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010821781.2
CDS	79..3162
Translation	MSFSGADALLGAFAPLHGGGSLHYALARKGGAGGARSAAGSSSGFHSWARTSVSSVSASPSRFRGAGTTSSTDSLDTLSNGPEGCVVVARSEKEQLQVLNDRFAGYIDKVRQLEAHNRSLEGEAAALRQQQAGRAAMGELYEREVREMRGAVLRLGAARGQLRLEQEHLLEDIAHVRQRLDDEARQREEAEAATRALARFAQEAEAARVELQKKAQALQEECGYLRRHHQEEVGELLGQIQSSSAAQTQAEARDALKCDVTSALREIRAQLEGHTVQSTLQSEEWFRVRLDRLSEAAKVNTDAMRSAQEEISEYRRQLQARTTELETLKSTKDSLERQRSELEDRHQADIASYQEAIQQLDAELRNTKWEMAAQLREYQDLLNVKMALDIEIAAYRKLLEGEECRIGFGPSPFLLPEGLPKIPSVSTHIKVKSEEKIKMVEKSEKETVIVEEQTEEVQVTEEVTEEEEKEAKEEEGEAVKSPPAEEAASPEKEEAKSPAEAKSPEKAKSPVKEEAKSPQKEEAKSPAEVKSPEKAKSPVEAKSPEKAKSPVKEEAKSPEKAKSPVEAKSPEKAKSPMKEEAKSPEKVKSPVEAKSPEKEEAKSPEKAKSPEKAKSPVKEEAKSPEKAKSPVEAKSPVKEEAKSPEKAKSPVEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPAKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPAKEEAKSPEKAKSPEKPKSPVKEEAKSPEKPKSPVKEEAKSPEKPKSPVKEEAKSPEKAKSPVKDEAKAPGKEVPKKEEAKSPVKEEEKPQEVRAKEPPKKAEEEKAPAIPKAEEKKDSKKDEVPKKEAPKPEEKKEPAVEKPKEPKAEAKKEEAEDKKKATTPEKEAPAKGKEEAKPKEKTEVAKKEPDDAKPKEPSKAAEKPKKEETPAAPEKKDAKEGKAAEAKKPEEKPKTEAQAKEPGKEASTPGKAKAEKSSSTDQKDSRPAEKAAEDKATKGEK

Target ORF information:

RefSeq Version	XM_010823479.2
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofilament, heavy polypeptide (NEFH), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010823479.2

ORF Insert Sequence:

ATGAGCTTCA GCGGCGCCGA CGCGCTGCTG GGCGCCTTCG CGCCGCTCCA CGGAGGCGGC 
AGCTTGCACT ATGCACTGGC TCGCAAAGGC GGCGCCGGCG GAGCGCGCTC TGCAGCCGGC 
TCCTCCAGCG GATTCCACTC ATGGGCGCGG ACCTCCGTGA GCTCCGTGTC GGCCTCCCCG 
AGCCGCTTCC GAGGCGCCGG GACTACCTCC AGCACCGACT CGCTAGACAC CCTGAGCAAC 
GGACCGGAGG GCTGCGTGGT GGTGGCGCGC AGTGAGAAGG AGCAGCTGCA GGTGCTGAAC 
GACCGCTTCG CGGGCTACAT CGACAAGGTG CGGCAGCTCG AGGCGCATAA CCGCAGCCTG 
GAGGGCGAGG CGGCGGCGCT GCGGCAGCAG CAGGCGGGCC GCGCAGCCAT GGGCGAGCTG 
TACGAGCGCG AGGTGCGCGA GATGCGCGGC GCAGTGCTGC GCTTGGGCGC GGCTCGCGGC 
CAGCTGCGCC TGGAGCAGGA GCACCTGCTG GAGGACATCG CGCACGTGCG CCAGCGCCTG 
GACGACGAGG CCCGGCAGCG CGAGGAGGCC GAGGCAGCGA CGCGCGCACT GGCCCGCTTT 
GCGCAGGAGG CCGAGGCGGC GCGCGTCGAG CTGCAGAAGA AGGCACAAGC CCTGCAGGAA 
GAGTGCGGCT ACCTGCGGCG TCACCACCAG GAGGAGGTGG GCGAGCTGCT CGGCCAGATC 
CAGAGCAGCA GCGCCGCGCA GACGCAGGCC GAGGCGCGCG ATGCCCTCAA GTGCGACGTG 
ACGTCGGCCC TGCGCGAGAT CCGCGCGCAG CTTGAAGGCC ACACGGTGCA GAGCACGCTT 
CAGTCCGAGG AGTGGTTCCG AGTGAGGCTG GACCGACTGT CCGAGGCAGC CAAGGTGAAC 
ACAGACGCCA TGCGCTCAGC ACAGGAGGAG ATATCTGAGT ACCGGCGCCA GCTGCAGGCC 
AGGACCACAG AGCTGGAGAC GCTCAAAAGC ACCAAGGACT CACTGGAGAG GCAGCGCTCT 
GAGCTGGAGG ACCGTCACCA GGCTGACATC GCATCCTACC AGGAGGCCAT CCAGCAGCTG 
GATGCAGAGC TGAGGAACAC CAAGTGGGAG ATGGCAGCCC AGCTCCGAGA GTACCAGGAT 
CTACTCAATG TCAAGATGGC TCTGGACATT GAGATCGCTG CTTACAGAAA ACTCCTGGAG 
GGTGAAGAAT GTCGAATTGG CTTTGGCCCG AGTCCTTTCC TCCTTCCAGA AGGACTCCCC 
AAGATCCCCT CCGTGTCCAC TCACATCAAA GTCAAAAGTG AAGAGAAGAT CAAGATGGTA 
GAAAAGTCAG AGAAGGAAAC TGTGATTGTG GAGGAACAGA CAGAGGAGGT CCAAGTGACT 
GAAGAAGTGA CTGAAGAAGA AGAGAAAGAG GCCAAAGAGG AGGAAGGAGA AGCAGTAAAG 
TCTCCCCCAG CAGAAGAGGC TGCATCCCCA GAGAAGGAGG AGGCCAAGTC ACCAGCCGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAGG AGGCCAAGTC CCCACAGAAG 
GAAGAGGCCA AGTCACCAGC TGAGGTGAAG TCCCCTGAGA AGGCCAAGTC CCCCGTGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAAG AAGCCAAATC CCCAGAGAAG 
GCCAAGTCCC CAGTGGAGGC CAAGTCTCCA GAAAAGGCCA AGTCCCCCAT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGT CAAATCCCCA GTGGAGGCCA AATCCCCAGA GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GAGAAAGCCA AGTCCCCTGT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GTGGAAGCCA AGTCCCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCA GTGGAGGCCA AGTCGCCAGA GAAGGCCAAG 
TCTCCTGTGA AGGAGGAGGC CAAGTCCCCA GAGAAGGCCA AGTCTCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT GTGAAAGAGG AGGCCAAGTC CCCAGAGAAG 
GCCAAGTCTC CTGCGAAGGA GGAGGCCAAG TCGCCAGAGA AGGCCAAGTC TCCTGTGAAA 
GAGGAGGCCA AGTCCCCAGA GAAGGCCAAG TCTCCTGTGA AAGAGGAGGC CAAGTCCCCA 
GAGAAGGCCA AGTCTCCTGC GAAGGAGGAG GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA 
GAGAAGCCCA AAAGCCCTGT GAAGGAGGAG GCCAAGTCCC CAGAGAAGCC CAAAAGCCCT 
GTGAAGGAGG AGGCCAAGTC CCCAGAGAAG CCCAAAAGCC CTGTCAAGGA GGAGGCCAAA 
TCCCCAGAGA AGGCCAAGTC TCCTGTGAAG GATGAGGCCA AGGCTCCTGG GAAGGAGGTC 
CCAAAGAAGG AGGAGGCCAA GTCCCCTGTG AAGGAGGAAG AAAAACCCCA GGAGGTGAGA 
GCCAAAGAGC CCCCAAAGAA GGCAGAGGAA GAGAAAGCTC CAGCCATACC CAAAGCTGAG 
GAGAAGAAGG ACAGCAAGAA AGATGAGGTG CCCAAGAAGG AGGCTCCAAA GCCCGAGGAG 
AAGAAGGAGC CTGCTGTGGA AAAGCCCAAA GAACCCAAAG CTGAAGCCAA GAAGGAAGAG 
GCTGAAGATA AGAAAAAAGC AACCACCCCA GAGAAGGAGG CTCCTGCCAA GGGGAAGGAA 
GAGGCCAAGC CTAAAGAGAA GACTGAGGTG GCCAAGAAGG AACCAGATGA TGCCAAGCCC 
AAAGAACCCA GCAAAGCAGC AGAAAAGCCA AAGAAGGAAG AGACGCCGGC AGCACCGGAG 
AAAAAAGACG CCAAGGAGGG GAAGGCCGCA GAAGCCAAGA AGCCTGAGGA GAAACCCAAA 
ACAGAGGCCC AAGCCAAGGA ACCTGGCAAG GAGGCCTCCA CACCCGGGAA AGCCAAGGCT 
GAGAAATCCT CCAGCACAGA CCAGAAAGAC AGCAGACCTG CAGAGAAGGC CGCAGAAGAT 
AAGGCCACCA AGGGAGAGAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEFH ( XM_005218121.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005218178.1 Bos taurus neurofilament, heavy polypeptide (NEFH), transcript variant X6, mRNA.

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CloneID	OBa28077
Clone ID Related Accession (Same CDS sequence)	XM_005218121.3 , XM_010823479.2 , XM_005218121.2
Accession Version	XM_005218121.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3084bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neurofilament heavy polypeptide isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104458.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005218121.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCA GCGGCGCCGA CGCGCTGCTG GGCGCCTTCG CGCCGCTCCA CGGAGGCGGC 
AGCTTGCACT ATGCACTGGC TCGCAAAGGC GGCGCCGGCG GAGCGCGCTC TGCAGCCGGC 
TCCTCCAGCG GATTCCACTC ATGGGCGCGG ACCTCCGTGA GCTCCGTGTC GGCCTCCCCG 
AGCCGCTTCC GAGGCGCCGG GACTACCTCC AGCACCGACT CGCTAGACAC CCTGAGCAAC 
GGACCGGAGG GCTGCGTGGT GGTGGCGCGC AGTGAGAAGG AGCAGCTGCA GGTGCTGAAC 
GACCGCTTCG CGGGCTACAT CGACAAGGTG CGGCAGCTCG AGGCGCATAA CCGCAGCCTG 
GAGGGCGAGG CGGCGGCGCT GCGGCAGCAG CAGGCGGGCC GCGCAGCCAT GGGCGAGCTG 
TACGAGCGCG AGGTGCGCGA GATGCGCGGC GCAGTGCTGC GCTTGGGCGC GGCTCGCGGC 
CAGCTGCGCC TGGAGCAGGA GCACCTGCTG GAGGACATCG CGCACGTGCG CCAGCGCCTG 
GACGACGAGG CCCGGCAGCG CGAGGAGGCC GAGGCAGCGA CGCGCGCACT GGCCCGCTTT 
GCGCAGGAGG CCGAGGCGGC GCGCGTCGAG CTGCAGAAGA AGGCACAAGC CCTGCAGGAA 
GAGTGCGGCT ACCTGCGGCG TCACCACCAG GAGGAGGTGG GCGAGCTGCT CGGCCAGATC 
CAGAGCAGCA GCGCCGCGCA GACGCAGGCC GAGGCGCGCG ATGCCCTCAA GTGCGACGTG 
ACGTCGGCCC TGCGCGAGAT CCGCGCGCAG CTTGAAGGCC ACACGGTGCA GAGCACGCTT 
CAGTCCGAGG AGTGGTTCCG AGTGAGGCTG GACCGACTGT CCGAGGCAGC CAAGGTGAAC 
ACAGACGCCA TGCGCTCAGC ACAGGAGGAG ATATCTGAGT ACCGGCGCCA GCTGCAGGCC 
AGGACCACAG AGCTGGAGAC GCTCAAAAGC ACCAAGGACT CACTGGAGAG GCAGCGCTCT 
GAGCTGGAGG ACCGTCACCA GGCTGACATC GCATCCTACC AGGAGGCCAT CCAGCAGCTG 
GATGCAGAGC TGAGGAACAC CAAGTGGGAG ATGGCAGCCC AGCTCCGAGA GTACCAGGAT 
CTACTCAATG TCAAGATGGC TCTGGACATT GAGATCGCTG CTTACAGAAA ACTCCTGGAG 
GGTGAAGAAT GTCGAATTGG CTTTGGCCCG AGTCCTTTCC TCCTTCCAGA AGGACTCCCC 
AAGATCCCCT CCGTGTCCAC TCACATCAAA GTCAAAAGTG AAGAGAAGAT CAAGATGGTA 
GAAAAGTCAG AGAAGGAAAC TGTGATTGTG GAGGAACAGA CAGAGGAGGT CCAAGTGACT 
GAAGAAGTGA CTGAAGAAGA AGAGAAAGAG GCCAAAGAGG AGGAAGGAGA AGCAGTAAAG 
TCTCCCCCAG CAGAAGAGGC TGCATCCCCA GAGAAGGAGG AGGCCAAGTC ACCAGCCGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAGG AGGCCAAGTC CCCACAGAAG 
GAAGAGGCCA AGTCACCAGC TGAGGTGAAG TCCCCTGAGA AGGCCAAGTC CCCCGTGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAAG AAGCCAAATC CCCAGAGAAG 
GCCAAGTCCC CAGTGGAGGC CAAGTCTCCA GAAAAGGCCA AGTCCCCCAT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGT CAAATCCCCA GTGGAGGCCA AATCCCCAGA GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GAGAAAGCCA AGTCCCCTGT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GTGGAAGCCA AGTCCCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCA GTGGAGGCCA AGTCGCCAGA GAAGGCCAAG 
TCTCCTGTGA AGGAGGAGGC CAAGTCCCCA GAGAAGGCCA AGTCTCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT GTGAAAGAGG AGGCCAAGTC CCCAGAGAAG 
GCCAAGTCTC CTGCGAAGGA GGAGGCCAAG TCGCCAGAGA AGGCCAAGTC TCCTGTGAAA 
GAGGAGGCCA AGTCCCCAGA GAAGGCCAAG TCTCCTGTGA AAGAGGAGGC CAAGTCCCCA 
GAGAAGGCCA AGTCTCCTGC GAAGGAGGAG GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA 
GAGAAGCCCA AAAGCCCTGT GAAGGAGGAG GCCAAGTCCC CAGAGAAGCC CAAAAGCCCT 
GTGAAGGAGG AGGCCAAGTC CCCAGAGAAG CCCAAAAGCC CTGTCAAGGA GGAGGCCAAA 
TCCCCAGAGA AGGCCAAGTC TCCTGTGAAG GATGAGGCCA AGGCTCCTGG GAAGGAGGTC 
CCAAAGAAGG AGGAGGCCAA GTCCCCTGTG AAGGAGGAAG AAAAACCCCA GGAGGTGAGA 
GCCAAAGAGC CCCCAAAGAA GGCAGAGGAA GAGAAAGCTC CAGCCATACC CAAAGCTGAG 
GAGAAGAAGG ACAGCAAGAA AGATGAGGTG CCCAAGAAGG AGGCTCCAAA GCCCGAGGAG 
AAGAAGGAGC CTGCTGTGGA AAAGCCCAAA GAACCCAAAG CTGAAGCCAA GAAGGAAGAG 
GCTGAAGATA AGAAAAAAGC AACCACCCCA GAGAAGGAGG CTCCTGCCAA GGGGAAGGAA 
GAGGCCAAGC CTAAAGAGAA GACTGAGGTG GCCAAGAAGG AACCAGATGA TGCCAAGCCC 
AAAGAACCCA GCAAAGCAGC AGAAAAGCCA AAGAAGGAAG AGACGCCGGC AGCACCGGAG 
AAAAAAGACG CCAAGGAGGG GAAGGCCGCA GAAGCCAAGA AGCCTGAGGA GAAACCCAAA 
ACAGAGGCCC AAGCCAAGGA ACCTGGCAAG GAGGCCTCCA CACCCGGGAA AGCCAAGGCT 
GAGAAATCCT CCAGCACAGA CCAGAAAGAC AGCAGACCTG CAGAGAAGGC CGCAGAAGAT 
AAGGCCACCA AGGGAGAGAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005218178.1
CDS	79..3162
Translation	MSFSGADALLGAFAPLHGGGSLHYALARKGGAGGARSAAGSSSGFHSWARTSVSSVSASPSRFRGAGTTSSTDSLDTLSNGPEGCVVVARSEKEQLQVLNDRFAGYIDKVRQLEAHNRSLEGEAAALRQQQAGRAAMGELYEREVREMRGAVLRLGAARGQLRLEQEHLLEDIAHVRQRLDDEARQREEAEAATRALARFAQEAEAARVELQKKAQALQEECGYLRRHHQEEVGELLGQIQSSSAAQTQAEARDALKCDVTSALREIRAQLEGHTVQSTLQSEEWFRVRLDRLSEAAKVNTDAMRSAQEEISEYRRQLQARTTELETLKSTKDSLERQRSELEDRHQADIASYQEAIQQLDAELRNTKWEMAAQLREYQDLLNVKMALDIEIAAYRKLLEGEECRIGFGPSPFLLPEGLPKIPSVSTHIKVKSEEKIKMVEKSEKETVIVEEQTEEVQVTEEVTEEEEKEAKEEEGEAVKSPPAEEAASPEKEEAKSPAEAKSPEKAKSPVKEEAKSPQKEEAKSPAEVKSPEKAKSPVEAKSPEKAKSPVKEEAKSPEKAKSPVEAKSPEKAKSPMKEEAKSPEKVKSPVEAKSPEKEEAKSPEKAKSPEKAKSPVKEEAKSPEKAKSPVEAKSPVKEEAKSPEKAKSPVEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPAKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPAKEEAKSPEKAKSPEKPKSPVKEEAKSPEKPKSPVKEEAKSPEKPKSPVKEEAKSPEKAKSPVKDEAKAPGKEVPKKEEAKSPVKEEEKPQEVRAKEPPKKAEEEKAPAIPKAEEKKDSKKDEVPKKEAPKPEEKKEPAVEKPKEPKAEAKKEEAEDKKKATTPEKEAPAKGKEEAKPKEKTEVAKKEPDDAKPKEPSKAAEKPKKEETPAAPEKKDAKEGKAAEAKKPEEKPKTEAQAKEPGKEASTPGKAKAEKSSSTDQKDSRPAEKAAEDKATKGEK

Target ORF information:

RefSeq Version	XM_005218121.2
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofilament, heavy polypeptide (NEFH), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005218121.2

ORF Insert Sequence:

ATGAGCTTCA GCGGCGCCGA CGCGCTGCTG GGCGCCTTCG CGCCGCTCCA CGGAGGCGGC 
AGCTTGCACT ATGCACTGGC TCGCAAAGGC GGCGCCGGCG GAGCGCGCTC TGCAGCCGGC 
TCCTCCAGCG GATTCCACTC ATGGGCGCGG ACCTCCGTGA GCTCCGTGTC GGCCTCCCCG 
AGCCGCTTCC GAGGCGCCGG GACTACCTCC AGCACCGACT CGCTAGACAC CCTGAGCAAC 
GGACCGGAGG GCTGCGTGGT GGTGGCGCGC AGTGAGAAGG AGCAGCTGCA GGTGCTGAAC 
GACCGCTTCG CGGGCTACAT CGACAAGGTG CGGCAGCTCG AGGCGCATAA CCGCAGCCTG 
GAGGGCGAGG CGGCGGCGCT GCGGCAGCAG CAGGCGGGCC GCGCAGCCAT GGGCGAGCTG 
TACGAGCGCG AGGTGCGCGA GATGCGCGGC GCAGTGCTGC GCTTGGGCGC GGCTCGCGGC 
CAGCTGCGCC TGGAGCAGGA GCACCTGCTG GAGGACATCG CGCACGTGCG CCAGCGCCTG 
GACGACGAGG CCCGGCAGCG CGAGGAGGCC GAGGCAGCGA CGCGCGCACT GGCCCGCTTT 
GCGCAGGAGG CCGAGGCGGC GCGCGTCGAG CTGCAGAAGA AGGCACAAGC CCTGCAGGAA 
GAGTGCGGCT ACCTGCGGCG TCACCACCAG GAGGAGGTGG GCGAGCTGCT CGGCCAGATC 
CAGAGCAGCA GCGCCGCGCA GACGCAGGCC GAGGCGCGCG ATGCCCTCAA GTGCGACGTG 
ACGTCGGCCC TGCGCGAGAT CCGCGCGCAG CTTGAAGGCC ACACGGTGCA GAGCACGCTT 
CAGTCCGAGG AGTGGTTCCG AGTGAGGCTG GACCGACTGT CCGAGGCAGC CAAGGTGAAC 
ACAGACGCCA TGCGCTCAGC ACAGGAGGAG ATATCTGAGT ACCGGCGCCA GCTGCAGGCC 
AGGACCACAG AGCTGGAGAC GCTCAAAAGC ACCAAGGACT CACTGGAGAG GCAGCGCTCT 
GAGCTGGAGG ACCGTCACCA GGCTGACATC GCATCCTACC AGGAGGCCAT CCAGCAGCTG 
GATGCAGAGC TGAGGAACAC CAAGTGGGAG ATGGCAGCCC AGCTCCGAGA GTACCAGGAT 
CTACTCAATG TCAAGATGGC TCTGGACATT GAGATCGCTG CTTACAGAAA ACTCCTGGAG 
GGTGAAGAAT GTCGAATTGG CTTTGGCCCG AGTCCTTTCC TCCTTCCAGA AGGACTCCCC 
AAGATCCCCT CCGTGTCCAC TCACATCAAA GTCAAAAGTG AAGAGAAGAT CAAGATGGTA 
GAAAAGTCAG AGAAGGAAAC TGTGATTGTG GAGGAACAGA CAGAGGAGGT CCAAGTGACT 
GAAGAAGTGA CTGAAGAAGA AGAGAAAGAG GCCAAAGAGG AGGAAGGAGA AGCAGTAAAG 
TCTCCCCCAG CAGAAGAGGC TGCATCCCCA GAGAAGGAGG AGGCCAAGTC ACCAGCCGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAGG AGGCCAAGTC CCCACAGAAG 
GAAGAGGCCA AGTCACCAGC TGAGGTGAAG TCCCCTGAGA AGGCCAAGTC CCCCGTGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAAG AAGCCAAATC CCCAGAGAAG 
GCCAAGTCCC CAGTGGAGGC CAAGTCTCCA GAAAAGGCCA AGTCCCCCAT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGT CAAATCCCCA GTGGAGGCCA AATCCCCAGA GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GAGAAAGCCA AGTCCCCTGT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GTGGAAGCCA AGTCCCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCA GTGGAGGCCA AGTCGCCAGA GAAGGCCAAG 
TCTCCTGTGA AGGAGGAGGC CAAGTCCCCA GAGAAGGCCA AGTCTCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT GTGAAAGAGG AGGCCAAGTC CCCAGAGAAG 
GCCAAGTCTC CTGCGAAGGA GGAGGCCAAG TCGCCAGAGA AGGCCAAGTC TCCTGTGAAA 
GAGGAGGCCA AGTCCCCAGA GAAGGCCAAG TCTCCTGTGA AAGAGGAGGC CAAGTCCCCA 
GAGAAGGCCA AGTCTCCTGC GAAGGAGGAG GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA 
GAGAAGCCCA AAAGCCCTGT GAAGGAGGAG GCCAAGTCCC CAGAGAAGCC CAAAAGCCCT 
GTGAAGGAGG AGGCCAAGTC CCCAGAGAAG CCCAAAAGCC CTGTCAAGGA GGAGGCCAAA 
TCCCCAGAGA AGGCCAAGTC TCCTGTGAAG GATGAGGCCA AGGCTCCTGG GAAGGAGGTC 
CCAAAGAAGG AGGAGGCCAA GTCCCCTGTG AAGGAGGAAG AAAAACCCCA GGAGGTGAGA 
GCCAAAGAGC CCCCAAAGAA GGCAGAGGAA GAGAAAGCTC CAGCCATACC CAAAGCTGAG 
GAGAAGAAGG ACAGCAAGAA AGATGAGGTG CCCAAGAAGG AGGCTCCAAA GCCCGAGGAG 
AAGAAGGAGC CTGCTGTGGA AAAGCCCAAA GAACCCAAAG CTGAAGCCAA GAAGGAAGAG 
GCTGAAGATA AGAAAAAAGC AACCACCCCA GAGAAGGAGG CTCCTGCCAA GGGGAAGGAA 
GAGGCCAAGC CTAAAGAGAA GACTGAGGTG GCCAAGAAGG AACCAGATGA TGCCAAGCCC 
AAAGAACCCA GCAAAGCAGC AGAAAAGCCA AAGAAGGAAG AGACGCCGGC AGCACCGGAG 
AAAAAAGACG CCAAGGAGGG GAAGGCCGCA GAAGCCAAGA AGCCTGAGGA GAAACCCAAA 
ACAGAGGCCC AAGCCAAGGA ACCTGGCAAG GAGGCCTCCA CACCCGGGAA AGCCAAGGCT 
GAGAAATCCT CCAGCACAGA CCAGAAAGAC AGCAGACCTG CAGAGAAGGC CGCAGAAGAT 
AAGGCCACCA AGGGAGAGAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEFH ( XM_010823480.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010821782.2 Bos taurus neurofilament, heavy polypeptide (NEFH), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBa51617
Clone ID Related Accession (Same CDS sequence)	XM_010823480.2 , XM_010814139.1
Accession Version	XM_010823480.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3042bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neurofilament heavy polypeptide isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646373.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010823480.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCA GCGGCGCCGA CGCGCTGCTG GGCGCCTTCG CGCCGCTCCA CGGAGGCGGC 
AGCTTGCACT ATGCACTGGC TCGCAAAGGC GGCGCCGGCG GAGCGCGCTC TGCAGCCGGC 
TCCTCCAGCG GATTCCACTC ATGGGCGCGG ACCTCCGTGA GCTCCGTGTC GGCCTCCCCG 
AGCCGCTTCC GAGGCGCCGG GACTACCTCC AGCACCGACT CGCTAGACAC CCTGAGCAAC 
GGACCGGAGG GCTGCGTGGT GGTGGCGCGC AGTGAGAAGG AGCAGCTGCA GGTGCTGAAC 
GACCGCTTCG CGGGCTACAT CGACAAGGTG CGGCAGCTCG AGGCGCATAA CCGCAGCCTG 
GAGGGCGAGG CGGCGGCGCT GCGGCAGCAG CAGGCGGGCC GCGCAGCCAT GGGCGAGCTG 
TACGAGCGCG AGGTGCGCGA GATGCGCGGC GCAGTGCTGC GCTTGGGCGC GGCTCGCGGC 
CAGCTGCGCC TGGAGCAGGA GCACCTGCTG GAGGACATCG CGCACGTGCG CCAGCGCCTG 
GACGACGAGG CCCGGCAGCG CGAGGAGGCC GAGGCAGCGA CGCGCGCACT GGCCCGCTTT 
GCGCAGGAGG CCGAGGCGGC GCGCGTCGAG CTGCAGAAGA AGGCACAAGC CCTGCAGGAA 
GAGTGCGGCT ACCTGCGGCG TCACCACCAG GAGGAGGTGG GCGAGCTGCT CGGCCAGATC 
CAGAGCAGCA GCGCCGCGCA GACGCAGGCC GAGGCGCGCG ATGCCCTCAA GTGCGACGTG 
ACGTCGGCCC TGCGCGAGAT CCGCGCGCAG CTTGAAGGCC ACACGGTGCA GAGCACGCTT 
CAGTCCGAGG AGTGGTTCCG AGTGAGGCTG GACCGACTGT CCGAGGCAGC CAAGGTGAAC 
ACAGACGCCA TGCGCTCAGC ACAGGAGGAG ATATCTGAGT ACCGGCGCCA GCTGCAGGCC 
AGGACCACAG AGCTGGAGAC GCTCAAAAGC ACCAAGGACT CACTGGAGAG GCAGCGCTCT 
GAGCTGGAGG ACCGTCACCA GGCTGACATC GCATCCTACC AGGAGGCCAT CCAGCAGCTG 
GATGCAGAGC TGAGGAACAC CAAGTGGGAG ATGGCAGCCC AGCTCCGAGA GTACCAGGAT 
CTACTCAATG TCAAGATGGC TCTGGACATT GAGATCGCTG CTTACAGAAA ACTCCTGGAG 
GGTGAAGAAT GTCGAATTGG CTTTGGCCCG AGTCCTTTCC TCCTTCCAGA AGGACTCCCC 
AAGATCCCCT CCGTGTCCAC TCACATCAAA GTCAAAAGTG AAGAGAAGAT CAAGATGGTA 
GAAAAGTCAG AGAAGGAAAC TGTGATTGTG GAGGAACAGA CAGAGGAGGT CCAAGTGACT 
GAAGAAGTGA CTGAAGAAGA AGAGAAAGAG GCCAAAGAGG AGGAAGGAGA AGCAGTAAAG 
TCTCCCCCAG CAGAAGAGGC TGCATCCCCA GAGAAGGAGG AGGCCAAGTC ACCAGCCGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAGG AGGCCAAGTC CCCACAGAAG 
GAAGAGGCCA AGTCACCAGC TGAGGTGAAG TCCCCTGAGA AGGCCAAGTC CCCCGTGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAAG AAGCCAAATC CCCAGAGAAG 
GCCAAGTCCC CAGTGGAGGC CAAGTCTCCA GAAAAGGCCA AGTCCCCCAT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGT CAAATCCCCA GTGGAGGCCA AATCCCCAGA GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GAGAAAGCCA AGTCCCCTGT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GTGGAAGCCA AGTCCCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCA GTGGAGGCCA AGTCGCCAGA GAAGGCCAAG 
TCTCCTGTGA AGGAGGAGGC CAAGTCCCCA GAGAAGGCCA AGTCTCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT GCGAAGGAGG AGGCCAAGTC GCCAGAGAAG 
GCCAAGTCTC CTGTGAAAGA GGAGGCCAAG TCCCCAGAGA AGGCCAAGTC TCCTGTGAAA 
GAGGAGGCCA AGTCCCCAGA GAAGGCCAAG TCTCCTGCGA AGGAGGAGGC CAAGTCCCCA 
GAGAAGGCCA AGTCCCCAGA GAAGCCCAAA AGCCCTGTGA AGGAGGAGGC CAAGTCCCCA 
GAGAAGCCCA AAAGCCCTGT GAAGGAGGAG GCCAAGTCCC CAGAGAAGCC CAAAAGCCCT 
GTCAAGGAGG AGGCCAAATC CCCAGAGAAG GCCAAGTCTC CTGTGAAGGA TGAGGCCAAG 
GCTCCTGGGA AGGAGGTCCC AAAGAAGGAG GAGGCCAAGT CCCCTGTGAA GGAGGAAGAA 
AAACCCCAGG AGGTGAGAGC CAAAGAGCCC CCAAAGAAGG CAGAGGAAGA GAAAGCTCCA 
GCCATACCCA AAGCTGAGGA GAAGAAGGAC AGCAAGAAAG ATGAGGTGCC CAAGAAGGAG 
GCTCCAAAGC CCGAGGAGAA GAAGGAGCCT GCTGTGGAAA AGCCCAAAGA ACCCAAAGCT 
GAAGCCAAGA AGGAAGAGGC TGAAGATAAG AAAAAAGCAA CCACCCCAGA GAAGGAGGCT 
CCTGCCAAGG GGAAGGAAGA GGCCAAGCCT AAAGAGAAGA CTGAGGTGGC CAAGAAGGAA 
CCAGATGATG CCAAGCCCAA AGAACCCAGC AAAGCAGCAG AAAAGCCAAA GAAGGAAGAG 
ACGCCGGCAG CACCGGAGAA AAAAGACGCC AAGGAGGGGA AGGCCGCAGA AGCCAAGAAG 
CCTGAGGAGA AACCCAAAAC AGAGGCCCAA GCCAAGGAAC CTGGCAAGGA GGCCTCCACA 
CCCGGGAAAG CCAAGGCTGA GAAATCCTCC AGCACAGACC AGAAAGACAG CAGACCTGCA 
GAGAAGGCCG CAGAAGATAA GGCCACCAAG GGAGAGAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010821782.2
CDS	79..3120
Translation	MSFSGADALLGAFAPLHGGGSLHYALARKGGAGGARSAAGSSSGFHSWARTSVSSVSASPSRFRGAGTTSSTDSLDTLSNGPEGCVVVARSEKEQLQVLNDRFAGYIDKVRQLEAHNRSLEGEAAALRQQQAGRAAMGELYEREVREMRGAVLRLGAARGQLRLEQEHLLEDIAHVRQRLDDEARQREEAEAATRALARFAQEAEAARVELQKKAQALQEECGYLRRHHQEEVGELLGQIQSSSAAQTQAEARDALKCDVTSALREIRAQLEGHTVQSTLQSEEWFRVRLDRLSEAAKVNTDAMRSAQEEISEYRRQLQARTTELETLKSTKDSLERQRSELEDRHQADIASYQEAIQQLDAELRNTKWEMAAQLREYQDLLNVKMALDIEIAAYRKLLEGEECRIGFGPSPFLLPEGLPKIPSVSTHIKVKSEEKIKMVEKSEKETVIVEEQTEEVQVTEEVTEEEEKEAKEEEGEAVKSPPAEEAASPEKEEAKSPAEAKSPEKAKSPVKEEAKSPQKEEAKSPAEVKSPEKAKSPVEAKSPEKAKSPVKEEAKSPEKAKSPVEAKSPEKAKSPMKEEAKSPEKVKSPVEAKSPEKEEAKSPEKAKSPEKAKSPVKEEAKSPEKAKSPVEAKSPVKEEAKSPEKAKSPVEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPAKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPAKEEAKSPEKAKSPEKPKSPVKEEAKSPEKPKSPVKEEAKSPEKPKSPVKEEAKSPEKAKSPVKDEAKAPGKEVPKKEEAKSPVKEEEKPQEVRAKEPPKKAEEEKAPAIPKAEEKKDSKKDEVPKKEAPKPEEKKEPAVEKPKEPKAEAKKEEAEDKKKATTPEKEAPAKGKEEAKPKEKTEVAKKEPDDAKPKEPSKAAEKPKKEETPAAPEKKDAKEGKAAEAKKPEEKPKTEAQAKEPGKEASTPGKAKAEKSSSTDQKDSRPAEKAAEDKATKGEK

Target ORF information:

RefSeq Version	XM_010823480.2
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofilament, heavy polypeptide (NEFH), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010823480.2

ORF Insert Sequence:

ATGAGCTTCA GCGGCGCCGA CGCGCTGCTG GGCGCCTTCG CGCCGCTCCA CGGAGGCGGC 
AGCTTGCACT ATGCACTGGC TCGCAAAGGC GGCGCCGGCG GAGCGCGCTC TGCAGCCGGC 
TCCTCCAGCG GATTCCACTC ATGGGCGCGG ACCTCCGTGA GCTCCGTGTC GGCCTCCCCG 
AGCCGCTTCC GAGGCGCCGG GACTACCTCC AGCACCGACT CGCTAGACAC CCTGAGCAAC 
GGACCGGAGG GCTGCGTGGT GGTGGCGCGC AGTGAGAAGG AGCAGCTGCA GGTGCTGAAC 
GACCGCTTCG CGGGCTACAT CGACAAGGTG CGGCAGCTCG AGGCGCATAA CCGCAGCCTG 
GAGGGCGAGG CGGCGGCGCT GCGGCAGCAG CAGGCGGGCC GCGCAGCCAT GGGCGAGCTG 
TACGAGCGCG AGGTGCGCGA GATGCGCGGC GCAGTGCTGC GCTTGGGCGC GGCTCGCGGC 
CAGCTGCGCC TGGAGCAGGA GCACCTGCTG GAGGACATCG CGCACGTGCG CCAGCGCCTG 
GACGACGAGG CCCGGCAGCG CGAGGAGGCC GAGGCAGCGA CGCGCGCACT GGCCCGCTTT 
GCGCAGGAGG CCGAGGCGGC GCGCGTCGAG CTGCAGAAGA AGGCACAAGC CCTGCAGGAA 
GAGTGCGGCT ACCTGCGGCG TCACCACCAG GAGGAGGTGG GCGAGCTGCT CGGCCAGATC 
CAGAGCAGCA GCGCCGCGCA GACGCAGGCC GAGGCGCGCG ATGCCCTCAA GTGCGACGTG 
ACGTCGGCCC TGCGCGAGAT CCGCGCGCAG CTTGAAGGCC ACACGGTGCA GAGCACGCTT 
CAGTCCGAGG AGTGGTTCCG AGTGAGGCTG GACCGACTGT CCGAGGCAGC CAAGGTGAAC 
ACAGACGCCA TGCGCTCAGC ACAGGAGGAG ATATCTGAGT ACCGGCGCCA GCTGCAGGCC 
AGGACCACAG AGCTGGAGAC GCTCAAAAGC ACCAAGGACT CACTGGAGAG GCAGCGCTCT 
GAGCTGGAGG ACCGTCACCA GGCTGACATC GCATCCTACC AGGAGGCCAT CCAGCAGCTG 
GATGCAGAGC TGAGGAACAC CAAGTGGGAG ATGGCAGCCC AGCTCCGAGA GTACCAGGAT 
CTACTCAATG TCAAGATGGC TCTGGACATT GAGATCGCTG CTTACAGAAA ACTCCTGGAG 
GGTGAAGAAT GTCGAATTGG CTTTGGCCCG AGTCCTTTCC TCCTTCCAGA AGGACTCCCC 
AAGATCCCCT CCGTGTCCAC TCACATCAAA GTCAAAAGTG AAGAGAAGAT CAAGATGGTA 
GAAAAGTCAG AGAAGGAAAC TGTGATTGTG GAGGAACAGA CAGAGGAGGT CCAAGTGACT 
GAAGAAGTGA CTGAAGAAGA AGAGAAAGAG GCCAAAGAGG AGGAAGGAGA AGCAGTAAAG 
TCTCCCCCAG CAGAAGAGGC TGCATCCCCA GAGAAGGAGG AGGCCAAGTC ACCAGCCGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAGG AGGCCAAGTC CCCACAGAAG 
GAAGAGGCCA AGTCACCAGC TGAGGTGAAG TCCCCTGAGA AGGCCAAGTC CCCCGTGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAAG AAGCCAAATC CCCAGAGAAG 
GCCAAGTCCC CAGTGGAGGC CAAGTCTCCA GAAAAGGCCA AGTCCCCCAT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGT CAAATCCCCA GTGGAGGCCA AATCCCCAGA GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GAGAAAGCCA AGTCCCCTGT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GTGGAAGCCA AGTCCCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCA GTGGAGGCCA AGTCGCCAGA GAAGGCCAAG 
TCTCCTGTGA AGGAGGAGGC CAAGTCCCCA GAGAAGGCCA AGTCTCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT GCGAAGGAGG AGGCCAAGTC GCCAGAGAAG 
GCCAAGTCTC CTGTGAAAGA GGAGGCCAAG TCCCCAGAGA AGGCCAAGTC TCCTGTGAAA 
GAGGAGGCCA AGTCCCCAGA GAAGGCCAAG TCTCCTGCGA AGGAGGAGGC CAAGTCCCCA 
GAGAAGGCCA AGTCCCCAGA GAAGCCCAAA AGCCCTGTGA AGGAGGAGGC CAAGTCCCCA 
GAGAAGCCCA AAAGCCCTGT GAAGGAGGAG GCCAAGTCCC CAGAGAAGCC CAAAAGCCCT 
GTCAAGGAGG AGGCCAAATC CCCAGAGAAG GCCAAGTCTC CTGTGAAGGA TGAGGCCAAG 
GCTCCTGGGA AGGAGGTCCC AAAGAAGGAG GAGGCCAAGT CCCCTGTGAA GGAGGAAGAA 
AAACCCCAGG AGGTGAGAGC CAAAGAGCCC CCAAAGAAGG CAGAGGAAGA GAAAGCTCCA 
GCCATACCCA AAGCTGAGGA GAAGAAGGAC AGCAAGAAAG ATGAGGTGCC CAAGAAGGAG 
GCTCCAAAGC CCGAGGAGAA GAAGGAGCCT GCTGTGGAAA AGCCCAAAGA ACCCAAAGCT 
GAAGCCAAGA AGGAAGAGGC TGAAGATAAG AAAAAAGCAA CCACCCCAGA GAAGGAGGCT 
CCTGCCAAGG GGAAGGAAGA GGCCAAGCCT AAAGAGAAGA CTGAGGTGGC CAAGAAGGAA 
CCAGATGATG CCAAGCCCAA AGAACCCAGC AAAGCAGCAG AAAAGCCAAA GAAGGAAGAG 
ACGCCGGCAG CACCGGAGAA AAAAGACGCC AAGGAGGGGA AGGCCGCAGA AGCCAAGAAG 
CCTGAGGAGA AACCCAAAAC AGAGGCCCAA GCCAAGGAAC CTGGCAAGGA GGCCTCCACA 
CCCGGGAAAG CCAAGGCTGA GAAATCCTCC AGCACAGACC AGAAAGACAG CAGACCTGCA 
GAGAAGGCCG CAGAAGATAA GGCCACCAAG GGAGAGAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEFH ( XM_010814139.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010812441.1 Bos taurus neurofilament, heavy polypeptide (NEFH), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBa51617
Clone ID Related Accession (Same CDS sequence)	XM_010823480.2 , XM_010814139.1
Accession Version	XM_010814139.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3042bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neurofilament heavy polypeptide isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104458.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCA GCGGCGCCGA CGCGCTGCTG GGCGCCTTCG CGCCGCTCCA CGGAGGCGGC 
AGCTTGCACT ATGCACTGGC TCGCAAAGGC GGCGCCGGCG GAGCGCGCTC TGCAGCCGGC 
TCCTCCAGCG GATTCCACTC ATGGGCGCGG ACCTCCGTGA GCTCCGTGTC GGCCTCCCCG 
AGCCGCTTCC GAGGCGCCGG GACTACCTCC AGCACCGACT CGCTAGACAC CCTGAGCAAC 
GGACCGGAGG GCTGCGTGGT GGTGGCGCGC AGTGAGAAGG AGCAGCTGCA GGTGCTGAAC 
GACCGCTTCG CGGGCTACAT CGACAAGGTG CGGCAGCTCG AGGCGCATAA CCGCAGCCTG 
GAGGGCGAGG CGGCGGCGCT GCGGCAGCAG CAGGCGGGCC GCGCAGCCAT GGGCGAGCTG 
TACGAGCGCG AGGTGCGCGA GATGCGCGGC GCAGTGCTGC GCTTGGGCGC GGCTCGCGGC 
CAGCTGCGCC TGGAGCAGGA GCACCTGCTG GAGGACATCG CGCACGTGCG CCAGCGCCTG 
GACGACGAGG CCCGGCAGCG CGAGGAGGCC GAGGCAGCGA CGCGCGCACT GGCCCGCTTT 
GCGCAGGAGG CCGAGGCGGC GCGCGTCGAG CTGCAGAAGA AGGCACAAGC CCTGCAGGAA 
GAGTGCGGCT ACCTGCGGCG TCACCACCAG GAGGAGGTGG GCGAGCTGCT CGGCCAGATC 
CAGAGCAGCA GCGCCGCGCA GACGCAGGCC GAGGCGCGCG ATGCCCTCAA GTGCGACGTG 
ACGTCGGCCC TGCGCGAGAT CCGCGCGCAG CTTGAAGGCC ACACGGTGCA GAGCACGCTT 
CAGTCCGAGG AGTGGTTCCG AGTGAGGCTG GACCGACTGT CCGAGGCAGC CAAGGTGAAC 
ACAGACGCCA TGCGCTCAGC ACAGGAGGAG ATATCTGAGT ACCGGCGCCA GCTGCAGGCC 
AGGACCACAG AGCTGGAGAC GCTCAAAAGC ACCAAGGACT CACTGGAGAG GCAGCGCTCT 
GAGCTGGAGG ACCGTCACCA GGCTGACATC GCATCCTACC AGGAGGCCAT CCAGCAGCTG 
GATGCAGAGC TGAGGAACAC CAAGTGGGAG ATGGCAGCCC AGCTCCGAGA GTACCAGGAT 
CTACTCAATG TCAAGATGGC TCTGGACATT GAGATCGCTG CTTACAGAAA ACTCCTGGAG 
GGTGAAGAAT GTCGAATTGG CTTTGGCCCG AGTCCTTTCC TCCTTCCAGA AGGACTCCCC 
AAGATCCCCT CCGTGTCCAC TCACATCAAA GTCAAAAGTG AAGAGAAGAT CAAGATGGTA 
GAAAAGTCAG AGAAGGAAAC TGTGATTGTG GAGGAACAGA CAGAGGAGGT CCAAGTGACT 
GAAGAAGTGA CTGAAGAAGA AGAGAAAGAG GCCAAAGAGG AGGAAGGAGA AGCAGTAAAG 
TCTCCCCCAG CAGAAGAGGC TGCATCCCCA GAGAAGGAGG AGGCCAAGTC ACCAGCCGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAGG AGGCCAAGTC CCCACAGAAG 
GAAGAGGCCA AGTCACCAGC TGAGGTGAAG TCCCCTGAGA AGGCCAAGTC CCCCGTGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAAG AAGCCAAATC CCCAGAGAAG 
GCCAAGTCCC CAGTGGAGGC CAAGTCTCCA GAAAAGGCCA AGTCCCCCAT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGT CAAATCCCCA GTGGAGGCCA AATCCCCAGA GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GAGAAAGCCA AGTCCCCTGT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GTGGAAGCCA AGTCCCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCA GTGGAGGCCA AGTCGCCAGA GAAGGCCAAG 
TCTCCTGTGA AGGAGGAGGC CAAGTCCCCA GAGAAGGCCA AGTCTCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT GCGAAGGAGG AGGCCAAGTC GCCAGAGAAG 
GCCAAGTCTC CTGTGAAAGA GGAGGCCAAG TCCCCAGAGA AGGCCAAGTC TCCTGTGAAA 
GAGGAGGCCA AGTCCCCAGA GAAGGCCAAG TCTCCTGCGA AGGAGGAGGC CAAGTCCCCA 
GAGAAGGCCA AGTCCCCAGA GAAGCCCAAA AGCCCTGTGA AGGAGGAGGC CAAGTCCCCA 
GAGAAGCCCA AAAGCCCTGT GAAGGAGGAG GCCAAGTCCC CAGAGAAGCC CAAAAGCCCT 
GTCAAGGAGG AGGCCAAATC CCCAGAGAAG GCCAAGTCTC CTGTGAAGGA TGAGGCCAAG 
GCTCCTGGGA AGGAGGTCCC AAAGAAGGAG GAGGCCAAGT CCCCTGTGAA GGAGGAAGAA 
AAACCCCAGG AGGTGAGAGC CAAAGAGCCC CCAAAGAAGG CAGAGGAAGA GAAAGCTCCA 
GCCATACCCA AAGCTGAGGA GAAGAAGGAC AGCAAGAAAG ATGAGGTGCC CAAGAAGGAG 
GCTCCAAAGC CCGAGGAGAA GAAGGAGCCT GCTGTGGAAA AGCCCAAAGA ACCCAAAGCT 
GAAGCCAAGA AGGAAGAGGC TGAAGATAAG AAAAAAGCAA CCACCCCAGA GAAGGAGGCT 
CCTGCCAAGG GGAAGGAAGA GGCCAAGCCT AAAGAGAAGA CTGAGGTGGC CAAGAAGGAA 
CCAGATGATG CCAAGCCCAA AGAACCCAGC AAAGCAGCAG AAAAGCCAAA GAAGGAAGAG 
ACGCCGGCAG CACCGGAGAA AAAAGACGCC AAGGAGGGGA AGGCCGCAGA AGCCAAGAAG 
CCTGAGGAGA AACCCAAAAC AGAGGCCCAA GCCAAGGAAC CTGGCAAGGA GGCCTCCACA 
CCCGGGAAAG CCAAGGCTGA GAAATCCTCC AGCACAGACC AGAAAGACAG CAGACCTGCA 
GAGAAGGCCG CAGAAGATAA GGCCACCAAG GGAGAGAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010812441.1
CDS	79..3120
Translation	MSFSGADALLGAFAPLHGGGSLHYALARKGGAGGARSAAGSSSGFHSWARTSVSSVSASPSRFRGAGTTSSTDSLDTLSNGPEGCVVVARSEKEQLQVLNDRFAGYIDKVRQLEAHNRSLEGEAAALRQQQAGRAAMGELYEREVREMRGAVLRLGAARGQLRLEQEHLLEDIAHVRQRLDDEARQREEAEAATRALARFAQEAEAARVELQKKAQALQEECGYLRRHHQEEVGELLGQIQSSSAAQTQAEARDALKCDVTSALREIRAQLEGHTVQSTLQSEEWFRVRLDRLSEAAKVNTDAMRSAQEEISEYRRQLQARTTELETLKSTKDSLERQRSELEDRHQADIASYQEAIQQLDAELRNTKWEMAAQLREYQDLLNVKMALDIEIAAYRKLLEGEECRIGFGPSPFLLPEGLPKIPSVSTHIKVKSEEKIKMVEKSEKETVIVEEQTEEVQVTEEVTEEEEKEAKEEEGEAVKSPPAEEAASPEKEEAKSPAEAKSPEKAKSPVKEEAKSPQKEEAKSPAEVKSPEKAKSPVEAKSPEKAKSPVKEEAKSPEKAKSPVEAKSPEKAKSPMKEEAKSPEKVKSPVEAKSPEKEEAKSPEKAKSPEKAKSPVKEEAKSPEKAKSPVEAKSPVKEEAKSPEKAKSPVEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPAKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPAKEEAKSPEKAKSPEKPKSPVKEEAKSPEKPKSPVKEEAKSPEKPKSPVKEEAKSPEKAKSPVKDEAKAPGKEVPKKEEAKSPVKEEEKPQEVRAKEPPKKAEEEKAPAIPKAEEKKDSKKDEVPKKEAPKPEEKKEPAVEKPKEPKAEAKKEEAEDKKKATTPEKEAPAKGKEEAKPKEKTEVAKKEPDDAKPKEPSKAAEKPKKEETPAAPEKKDAKEGKAAEAKKPEEKPKTEAQAKEPGKEASTPGKAKAEKSSSTDQKDSRPAEKAAEDKATKGEK

Target ORF information:

RefSeq Version	XM_010814139.1
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofilament, heavy polypeptide (NEFH), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010814139.1

ORF Insert Sequence:

ATGAGCTTCA GCGGCGCCGA CGCGCTGCTG GGCGCCTTCG CGCCGCTCCA CGGAGGCGGC 
AGCTTGCACT ATGCACTGGC TCGCAAAGGC GGCGCCGGCG GAGCGCGCTC TGCAGCCGGC 
TCCTCCAGCG GATTCCACTC ATGGGCGCGG ACCTCCGTGA GCTCCGTGTC GGCCTCCCCG 
AGCCGCTTCC GAGGCGCCGG GACTACCTCC AGCACCGACT CGCTAGACAC CCTGAGCAAC 
GGACCGGAGG GCTGCGTGGT GGTGGCGCGC AGTGAGAAGG AGCAGCTGCA GGTGCTGAAC 
GACCGCTTCG CGGGCTACAT CGACAAGGTG CGGCAGCTCG AGGCGCATAA CCGCAGCCTG 
GAGGGCGAGG CGGCGGCGCT GCGGCAGCAG CAGGCGGGCC GCGCAGCCAT GGGCGAGCTG 
TACGAGCGCG AGGTGCGCGA GATGCGCGGC GCAGTGCTGC GCTTGGGCGC GGCTCGCGGC 
CAGCTGCGCC TGGAGCAGGA GCACCTGCTG GAGGACATCG CGCACGTGCG CCAGCGCCTG 
GACGACGAGG CCCGGCAGCG CGAGGAGGCC GAGGCAGCGA CGCGCGCACT GGCCCGCTTT 
GCGCAGGAGG CCGAGGCGGC GCGCGTCGAG CTGCAGAAGA AGGCACAAGC CCTGCAGGAA 
GAGTGCGGCT ACCTGCGGCG TCACCACCAG GAGGAGGTGG GCGAGCTGCT CGGCCAGATC 
CAGAGCAGCA GCGCCGCGCA GACGCAGGCC GAGGCGCGCG ATGCCCTCAA GTGCGACGTG 
ACGTCGGCCC TGCGCGAGAT CCGCGCGCAG CTTGAAGGCC ACACGGTGCA GAGCACGCTT 
CAGTCCGAGG AGTGGTTCCG AGTGAGGCTG GACCGACTGT CCGAGGCAGC CAAGGTGAAC 
ACAGACGCCA TGCGCTCAGC ACAGGAGGAG ATATCTGAGT ACCGGCGCCA GCTGCAGGCC 
AGGACCACAG AGCTGGAGAC GCTCAAAAGC ACCAAGGACT CACTGGAGAG GCAGCGCTCT 
GAGCTGGAGG ACCGTCACCA GGCTGACATC GCATCCTACC AGGAGGCCAT CCAGCAGCTG 
GATGCAGAGC TGAGGAACAC CAAGTGGGAG ATGGCAGCCC AGCTCCGAGA GTACCAGGAT 
CTACTCAATG TCAAGATGGC TCTGGACATT GAGATCGCTG CTTACAGAAA ACTCCTGGAG 
GGTGAAGAAT GTCGAATTGG CTTTGGCCCG AGTCCTTTCC TCCTTCCAGA AGGACTCCCC 
AAGATCCCCT CCGTGTCCAC TCACATCAAA GTCAAAAGTG AAGAGAAGAT CAAGATGGTA 
GAAAAGTCAG AGAAGGAAAC TGTGATTGTG GAGGAACAGA CAGAGGAGGT CCAAGTGACT 
GAAGAAGTGA CTGAAGAAGA AGAGAAAGAG GCCAAAGAGG AGGAAGGAGA AGCAGTAAAG 
TCTCCCCCAG CAGAAGAGGC TGCATCCCCA GAGAAGGAGG AGGCCAAGTC ACCAGCCGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAGG AGGCCAAGTC CCCACAGAAG 
GAAGAGGCCA AGTCACCAGC TGAGGTGAAG TCCCCTGAGA AGGCCAAGTC CCCCGTGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAAG AAGCCAAATC CCCAGAGAAG 
GCCAAGTCCC CAGTGGAGGC CAAGTCTCCA GAAAAGGCCA AGTCCCCCAT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGT CAAATCCCCA GTGGAGGCCA AATCCCCAGA GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GAGAAAGCCA AGTCCCCTGT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GTGGAAGCCA AGTCCCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCA GTGGAGGCCA AGTCGCCAGA GAAGGCCAAG 
TCTCCTGTGA AGGAGGAGGC CAAGTCCCCA GAGAAGGCCA AGTCTCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT GCGAAGGAGG AGGCCAAGTC GCCAGAGAAG 
GCCAAGTCTC CTGTGAAAGA GGAGGCCAAG TCCCCAGAGA AGGCCAAGTC TCCTGTGAAA 
GAGGAGGCCA AGTCCCCAGA GAAGGCCAAG TCTCCTGCGA AGGAGGAGGC CAAGTCCCCA 
GAGAAGGCCA AGTCCCCAGA GAAGCCCAAA AGCCCTGTGA AGGAGGAGGC CAAGTCCCCA 
GAGAAGCCCA AAAGCCCTGT GAAGGAGGAG GCCAAGTCCC CAGAGAAGCC CAAAAGCCCT 
GTCAAGGAGG AGGCCAAATC CCCAGAGAAG GCCAAGTCTC CTGTGAAGGA TGAGGCCAAG 
GCTCCTGGGA AGGAGGTCCC AAAGAAGGAG GAGGCCAAGT CCCCTGTGAA GGAGGAAGAA 
AAACCCCAGG AGGTGAGAGC CAAAGAGCCC CCAAAGAAGG CAGAGGAAGA GAAAGCTCCA 
GCCATACCCA AAGCTGAGGA GAAGAAGGAC AGCAAGAAAG ATGAGGTGCC CAAGAAGGAG 
GCTCCAAAGC CCGAGGAGAA GAAGGAGCCT GCTGTGGAAA AGCCCAAAGA ACCCAAAGCT 
GAAGCCAAGA AGGAAGAGGC TGAAGATAAG AAAAAAGCAA CCACCCCAGA GAAGGAGGCT 
CCTGCCAAGG GGAAGGAAGA GGCCAAGCCT AAAGAGAAGA CTGAGGTGGC CAAGAAGGAA 
CCAGATGATG CCAAGCCCAA AGAACCCAGC AAAGCAGCAG AAAAGCCAAA GAAGGAAGAG 
ACGCCGGCAG CACCGGAGAA AAAAGACGCC AAGGAGGGGA AGGCCGCAGA AGCCAAGAAG 
CCTGAGGAGA AACCCAAAAC AGAGGCCCAA GCCAAGGAAC CTGGCAAGGA GGCCTCCACA 
CCCGGGAAAG CCAAGGCTGA GAAATCCTCC AGCACAGACC AGAAAGACAG CAGACCTGCA 
GAGAAGGCCG CAGAAGATAA GGCCACCAAG GGAGAGAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEFH ( XM_010814141.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010812443.1 Bos taurus neurofilament, heavy polypeptide (NEFH), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBa51618
Clone ID Related Accession (Same CDS sequence)	XM_010814141.1 , XM_010823481.2
Accession Version	XM_010814141.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2958bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neurofilament heavy polypeptide isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104458.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCA GCGGCGCCGA CGCGCTGCTG GGCGCCTTCG CGCCGCTCCA CGGAGGCGGC 
AGCTTGCACT ATGCACTGGC TCGCAAAGGC GGCGCCGGCG GAGCGCGCTC TGCAGCCGGC 
TCCTCCAGCG GATTCCACTC ATGGGCGCGG ACCTCCGTGA GCTCCGTGTC GGCCTCCCCG 
AGCCGCTTCC GAGGCGCCGG GACTACCTCC AGCACCGACT CGCTAGACAC CCTGAGCAAC 
GGACCGGAGG GCTGCGTGGT GGTGGCGCGC AGTGAGAAGG AGCAGCTGCA GGTGCTGAAC 
GACCGCTTCG CGGGCTACAT CGACAAGGTG CGGCAGCTCG AGGCGCATAA CCGCAGCCTG 
GAGGGCGAGG CGGCGGCGCT GCGGCAGCAG CAGGCGGGCC GCGCAGCCAT GGGCGAGCTG 
TACGAGCGCG AGGTGCGCGA GATGCGCGGC GCAGTGCTGC GCTTGGGCGC GGCTCGCGGC 
CAGCTGCGCC TGGAGCAGGA GCACCTGCTG GAGGACATCG CGCACGTGCG CCAGCGCCTG 
GACGACGAGG CCCGGCAGCG CGAGGAGGCC GAGGCAGCGA CGCGCGCACT GGCCCGCTTT 
GCGCAGGAGG CCGAGGCGGC GCGCGTCGAG CTGCAGAAGA AGGCACAAGC CCTGCAGGAA 
GAGTGCGGCT ACCTGCGGCG TCACCACCAG GAGGAGGTGG GCGAGCTGCT CGGCCAGATC 
CAGAGCAGCA GCGCCGCGCA GACGCAGGCC GAGGCGCGCG ATGCCCTCAA GTGCGACGTG 
ACGTCGGCCC TGCGCGAGAT CCGCGCGCAG CTTGAAGGCC ACACGGTGCA GAGCACGCTT 
CAGTCCGAGG AGTGGTTCCG AGTGAGGCTG GACCGACTGT CCGAGGCAGC CAAGGTGAAC 
ACAGACGCCA TGCGCTCAGC ACAGGAGGAG ATATCTGAGT ACCGGCGCCA GCTGCAGGCC 
AGGACCACAG AGCTGGAGAC GCTCAAAAGC ACCAAGGACT CACTGGAGAG GCAGCGCTCT 
GAGCTGGAGG ACCGTCACCA GGCTGACATC GCATCCTACC AGGAGGCCAT CCAGCAGCTG 
GATGCAGAGC TGAGGAACAC CAAGTGGGAG ATGGCAGCCC AGCTCCGAGA GTACCAGGAT 
CTACTCAATG TCAAGATGGC TCTGGACATT GAGATCGCTG CTTACAGAAA ACTCCTGGAG 
GGTGAAGAAT GTCGAATTGG CTTTGGCCCG AGTCCTTTCC TCCTTCCAGA AGGACTCCCC 
AAGATCCCCT CCGTGTCCAC TCACATCAAA GTCAAAAGTG AAGAGAAGAT CAAGATGGTA 
GAAAAGTCAG AGAAGGAAAC TGTGATTGTG GAGGAACAGA CAGAGGAGGT CCAAGTGACT 
GAAGAAGTGA CTGAAGAAGA AGAGAAAGAG GCCAAAGAGG AGGAAGGAGA AGCAGTAAAG 
TCTCCCCCAG CAGAAGAGGC TGCATCCCCA GAGAAGGAGG AGGCCAAGTC ACCAGCCGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAGG AGGCCAAGTC CCCACAGAAG 
GAAGAGGCCA AGTCACCAGC TGAGGTGAAG TCCCCTGAGA AGGCCAAGTC CCCCGTGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAAG AAGCCAAATC CCCAGAGAAG 
GCCAAGTCCC CAGTGGAGGC CAAGTCTCCA GAAAAGGCCA AGTCCCCCAT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGT CAAATCCCCA GTGGAGGCCA AATCCCCAGA GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GAGAAAGCCA AGTCCCCTGT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GTGGAAGCCA AGTCCCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCA GTGGAGGCCA AGTCGCCAGA GAAGGCCAAG 
TCTCCTGTGA AGGAGGAGGC CAAGTCCCCA GAGAAGGCCA AGTCTCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT GTGAAAGAGG AGGCCAAGTC CCCAGAGAAG 
GCCAAGTCTC CTGCGAAGGA GGAGGCCAAG TCCCCAGAGA AGGCCAAGTC CCCAGAGAAG 
CCCAAAAGCC CTGTGAAGGA GGAGGCCAAG TCCCCAGAGA AGCCCAAAAG CCCTGTGAAG 
GAGGAGGCCA AGTCCCCAGA GAAGCCCAAA AGCCCTGTCA AGGAGGAGGC CAAATCCCCA 
GAGAAGGCCA AGTCTCCTGT GAAGGATGAG GCCAAGGCTC CTGGGAAGGA GGTCCCAAAG 
AAGGAGGAGG CCAAGTCCCC TGTGAAGGAG GAAGAAAAAC CCCAGGAGGT GAGAGCCAAA 
GAGCCCCCAA AGAAGGCAGA GGAAGAGAAA GCTCCAGCCA TACCCAAAGC TGAGGAGAAG 
AAGGACAGCA AGAAAGATGA GGTGCCCAAG AAGGAGGCTC CAAAGCCCGA GGAGAAGAAG 
GAGCCTGCTG TGGAAAAGCC CAAAGAACCC AAAGCTGAAG CCAAGAAGGA AGAGGCTGAA 
GATAAGAAAA AAGCAACCAC CCCAGAGAAG GAGGCTCCTG CCAAGGGGAA GGAAGAGGCC 
AAGCCTAAAG AGAAGACTGA GGTGGCCAAG AAGGAACCAG ATGATGCCAA GCCCAAAGAA 
CCCAGCAAAG CAGCAGAAAA GCCAAAGAAG GAAGAGACGC CGGCAGCACC GGAGAAAAAA 
GACGCCAAGG AGGGGAAGGC CGCAGAAGCC AAGAAGCCTG AGGAGAAACC CAAAACAGAG 
GCCCAAGCCA AGGAACCTGG CAAGGAGGCC TCCACACCCG GGAAAGCCAA GGCTGAGAAA 
TCCTCCAGCA CAGACCAGAA AGACAGCAGA CCTGCAGAGA AGGCCGCAGA AGATAAGGCC 
ACCAAGGGAG AGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010812443.1
CDS	79..3036
Translation	MSFSGADALLGAFAPLHGGGSLHYALARKGGAGGARSAAGSSSGFHSWARTSVSSVSASPSRFRGAGTTSSTDSLDTLSNGPEGCVVVARSEKEQLQVLNDRFAGYIDKVRQLEAHNRSLEGEAAALRQQQAGRAAMGELYEREVREMRGAVLRLGAARGQLRLEQEHLLEDIAHVRQRLDDEARQREEAEAATRALARFAQEAEAARVELQKKAQALQEECGYLRRHHQEEVGELLGQIQSSSAAQTQAEARDALKCDVTSALREIRAQLEGHTVQSTLQSEEWFRVRLDRLSEAAKVNTDAMRSAQEEISEYRRQLQARTTELETLKSTKDSLERQRSELEDRHQADIASYQEAIQQLDAELRNTKWEMAAQLREYQDLLNVKMALDIEIAAYRKLLEGEECRIGFGPSPFLLPEGLPKIPSVSTHIKVKSEEKIKMVEKSEKETVIVEEQTEEVQVTEEVTEEEEKEAKEEEGEAVKSPPAEEAASPEKEEAKSPAEAKSPEKAKSPVKEEAKSPQKEEAKSPAEVKSPEKAKSPVEAKSPEKAKSPVKEEAKSPEKAKSPVEAKSPEKAKSPMKEEAKSPEKVKSPVEAKSPEKEEAKSPEKAKSPEKAKSPVKEEAKSPEKAKSPVEAKSPVKEEAKSPEKAKSPVEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPAKEEAKSPEKAKSPEKPKSPVKEEAKSPEKPKSPVKEEAKSPEKPKSPVKEEAKSPEKAKSPVKDEAKAPGKEVPKKEEAKSPVKEEEKPQEVRAKEPPKKAEEEKAPAIPKAEEKKDSKKDEVPKKEAPKPEEKKEPAVEKPKEPKAEAKKEEAEDKKKATTPEKEAPAKGKEEAKPKEKTEVAKKEPDDAKPKEPSKAAEKPKKEETPAAPEKKDAKEGKAAEAKKPEEKPKTEAQAKEPGKEASTPGKAKAEKSSSTDQKDSRPAEKAAEDKATKGEK

Target ORF information:

RefSeq Version	XM_010814141.1
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofilament, heavy polypeptide (NEFH), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010814141.1

ORF Insert Sequence:

ATGAGCTTCA GCGGCGCCGA CGCGCTGCTG GGCGCCTTCG CGCCGCTCCA CGGAGGCGGC 
AGCTTGCACT ATGCACTGGC TCGCAAAGGC GGCGCCGGCG GAGCGCGCTC TGCAGCCGGC 
TCCTCCAGCG GATTCCACTC ATGGGCGCGG ACCTCCGTGA GCTCCGTGTC GGCCTCCCCG 
AGCCGCTTCC GAGGCGCCGG GACTACCTCC AGCACCGACT CGCTAGACAC CCTGAGCAAC 
GGACCGGAGG GCTGCGTGGT GGTGGCGCGC AGTGAGAAGG AGCAGCTGCA GGTGCTGAAC 
GACCGCTTCG CGGGCTACAT CGACAAGGTG CGGCAGCTCG AGGCGCATAA CCGCAGCCTG 
GAGGGCGAGG CGGCGGCGCT GCGGCAGCAG CAGGCGGGCC GCGCAGCCAT GGGCGAGCTG 
TACGAGCGCG AGGTGCGCGA GATGCGCGGC GCAGTGCTGC GCTTGGGCGC GGCTCGCGGC 
CAGCTGCGCC TGGAGCAGGA GCACCTGCTG GAGGACATCG CGCACGTGCG CCAGCGCCTG 
GACGACGAGG CCCGGCAGCG CGAGGAGGCC GAGGCAGCGA CGCGCGCACT GGCCCGCTTT 
GCGCAGGAGG CCGAGGCGGC GCGCGTCGAG CTGCAGAAGA AGGCACAAGC CCTGCAGGAA 
GAGTGCGGCT ACCTGCGGCG TCACCACCAG GAGGAGGTGG GCGAGCTGCT CGGCCAGATC 
CAGAGCAGCA GCGCCGCGCA GACGCAGGCC GAGGCGCGCG ATGCCCTCAA GTGCGACGTG 
ACGTCGGCCC TGCGCGAGAT CCGCGCGCAG CTTGAAGGCC ACACGGTGCA GAGCACGCTT 
CAGTCCGAGG AGTGGTTCCG AGTGAGGCTG GACCGACTGT CCGAGGCAGC CAAGGTGAAC 
ACAGACGCCA TGCGCTCAGC ACAGGAGGAG ATATCTGAGT ACCGGCGCCA GCTGCAGGCC 
AGGACCACAG AGCTGGAGAC GCTCAAAAGC ACCAAGGACT CACTGGAGAG GCAGCGCTCT 
GAGCTGGAGG ACCGTCACCA GGCTGACATC GCATCCTACC AGGAGGCCAT CCAGCAGCTG 
GATGCAGAGC TGAGGAACAC CAAGTGGGAG ATGGCAGCCC AGCTCCGAGA GTACCAGGAT 
CTACTCAATG TCAAGATGGC TCTGGACATT GAGATCGCTG CTTACAGAAA ACTCCTGGAG 
GGTGAAGAAT GTCGAATTGG CTTTGGCCCG AGTCCTTTCC TCCTTCCAGA AGGACTCCCC 
AAGATCCCCT CCGTGTCCAC TCACATCAAA GTCAAAAGTG AAGAGAAGAT CAAGATGGTA 
GAAAAGTCAG AGAAGGAAAC TGTGATTGTG GAGGAACAGA CAGAGGAGGT CCAAGTGACT 
GAAGAAGTGA CTGAAGAAGA AGAGAAAGAG GCCAAAGAGG AGGAAGGAGA AGCAGTAAAG 
TCTCCCCCAG CAGAAGAGGC TGCATCCCCA GAGAAGGAGG AGGCCAAGTC ACCAGCCGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAGG AGGCCAAGTC CCCACAGAAG 
GAAGAGGCCA AGTCACCAGC TGAGGTGAAG TCCCCTGAGA AGGCCAAGTC CCCCGTGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAAG AAGCCAAATC CCCAGAGAAG 
GCCAAGTCCC CAGTGGAGGC CAAGTCTCCA GAAAAGGCCA AGTCCCCCAT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGT CAAATCCCCA GTGGAGGCCA AATCCCCAGA GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GAGAAAGCCA AGTCCCCTGT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GTGGAAGCCA AGTCCCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCA GTGGAGGCCA AGTCGCCAGA GAAGGCCAAG 
TCTCCTGTGA AGGAGGAGGC CAAGTCCCCA GAGAAGGCCA AGTCTCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT GTGAAAGAGG AGGCCAAGTC CCCAGAGAAG 
GCCAAGTCTC CTGCGAAGGA GGAGGCCAAG TCCCCAGAGA AGGCCAAGTC CCCAGAGAAG 
CCCAAAAGCC CTGTGAAGGA GGAGGCCAAG TCCCCAGAGA AGCCCAAAAG CCCTGTGAAG 
GAGGAGGCCA AGTCCCCAGA GAAGCCCAAA AGCCCTGTCA AGGAGGAGGC CAAATCCCCA 
GAGAAGGCCA AGTCTCCTGT GAAGGATGAG GCCAAGGCTC CTGGGAAGGA GGTCCCAAAG 
AAGGAGGAGG CCAAGTCCCC TGTGAAGGAG GAAGAAAAAC CCCAGGAGGT GAGAGCCAAA 
GAGCCCCCAA AGAAGGCAGA GGAAGAGAAA GCTCCAGCCA TACCCAAAGC TGAGGAGAAG 
AAGGACAGCA AGAAAGATGA GGTGCCCAAG AAGGAGGCTC CAAAGCCCGA GGAGAAGAAG 
GAGCCTGCTG TGGAAAAGCC CAAAGAACCC AAAGCTGAAG CCAAGAAGGA AGAGGCTGAA 
GATAAGAAAA AAGCAACCAC CCCAGAGAAG GAGGCTCCTG CCAAGGGGAA GGAAGAGGCC 
AAGCCTAAAG AGAAGACTGA GGTGGCCAAG AAGGAACCAG ATGATGCCAA GCCCAAAGAA 
CCCAGCAAAG CAGCAGAAAA GCCAAAGAAG GAAGAGACGC CGGCAGCACC GGAGAAAAAA 
GACGCCAAGG AGGGGAAGGC CGCAGAAGCC AAGAAGCCTG AGGAGAAACC CAAAACAGAG 
GCCCAAGCCA AGGAACCTGG CAAGGAGGCC TCCACACCCG GGAAAGCCAA GGCTGAGAAA 
TCCTCCAGCA CAGACCAGAA AGACAGCAGA CCTGCAGAGA AGGCCGCAGA AGATAAGGCC 
ACCAAGGGAG AGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEFH ( XM_010823481.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010821783.2 Bos taurus neurofilament, heavy polypeptide (NEFH), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBa51618
Clone ID Related Accession (Same CDS sequence)	XM_010814141.1 , XM_010823481.2
Accession Version	XM_010823481.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2958bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neurofilament heavy polypeptide isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646373.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010823481.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCA GCGGCGCCGA CGCGCTGCTG GGCGCCTTCG CGCCGCTCCA CGGAGGCGGC 
AGCTTGCACT ATGCACTGGC TCGCAAAGGC GGCGCCGGCG GAGCGCGCTC TGCAGCCGGC 
TCCTCCAGCG GATTCCACTC ATGGGCGCGG ACCTCCGTGA GCTCCGTGTC GGCCTCCCCG 
AGCCGCTTCC GAGGCGCCGG GACTACCTCC AGCACCGACT CGCTAGACAC CCTGAGCAAC 
GGACCGGAGG GCTGCGTGGT GGTGGCGCGC AGTGAGAAGG AGCAGCTGCA GGTGCTGAAC 
GACCGCTTCG CGGGCTACAT CGACAAGGTG CGGCAGCTCG AGGCGCATAA CCGCAGCCTG 
GAGGGCGAGG CGGCGGCGCT GCGGCAGCAG CAGGCGGGCC GCGCAGCCAT GGGCGAGCTG 
TACGAGCGCG AGGTGCGCGA GATGCGCGGC GCAGTGCTGC GCTTGGGCGC GGCTCGCGGC 
CAGCTGCGCC TGGAGCAGGA GCACCTGCTG GAGGACATCG CGCACGTGCG CCAGCGCCTG 
GACGACGAGG CCCGGCAGCG CGAGGAGGCC GAGGCAGCGA CGCGCGCACT GGCCCGCTTT 
GCGCAGGAGG CCGAGGCGGC GCGCGTCGAG CTGCAGAAGA AGGCACAAGC CCTGCAGGAA 
GAGTGCGGCT ACCTGCGGCG TCACCACCAG GAGGAGGTGG GCGAGCTGCT CGGCCAGATC 
CAGAGCAGCA GCGCCGCGCA GACGCAGGCC GAGGCGCGCG ATGCCCTCAA GTGCGACGTG 
ACGTCGGCCC TGCGCGAGAT CCGCGCGCAG CTTGAAGGCC ACACGGTGCA GAGCACGCTT 
CAGTCCGAGG AGTGGTTCCG AGTGAGGCTG GACCGACTGT CCGAGGCAGC CAAGGTGAAC 
ACAGACGCCA TGCGCTCAGC ACAGGAGGAG ATATCTGAGT ACCGGCGCCA GCTGCAGGCC 
AGGACCACAG AGCTGGAGAC GCTCAAAAGC ACCAAGGACT CACTGGAGAG GCAGCGCTCT 
GAGCTGGAGG ACCGTCACCA GGCTGACATC GCATCCTACC AGGAGGCCAT CCAGCAGCTG 
GATGCAGAGC TGAGGAACAC CAAGTGGGAG ATGGCAGCCC AGCTCCGAGA GTACCAGGAT 
CTACTCAATG TCAAGATGGC TCTGGACATT GAGATCGCTG CTTACAGAAA ACTCCTGGAG 
GGTGAAGAAT GTCGAATTGG CTTTGGCCCG AGTCCTTTCC TCCTTCCAGA AGGACTCCCC 
AAGATCCCCT CCGTGTCCAC TCACATCAAA GTCAAAAGTG AAGAGAAGAT CAAGATGGTA 
GAAAAGTCAG AGAAGGAAAC TGTGATTGTG GAGGAACAGA CAGAGGAGGT CCAAGTGACT 
GAAGAAGTGA CTGAAGAAGA AGAGAAAGAG GCCAAAGAGG AGGAAGGAGA AGCAGTAAAG 
TCTCCCCCAG CAGAAGAGGC TGCATCCCCA GAGAAGGAGG AGGCCAAGTC ACCAGCCGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAGG AGGCCAAGTC CCCACAGAAG 
GAAGAGGCCA AGTCACCAGC TGAGGTGAAG TCCCCTGAGA AGGCCAAGTC CCCCGTGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAAG AAGCCAAATC CCCAGAGAAG 
GCCAAGTCCC CAGTGGAGGC CAAGTCTCCA GAAAAGGCCA AGTCCCCCAT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGT CAAATCCCCA GTGGAGGCCA AATCCCCAGA GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GAGAAAGCCA AGTCCCCTGT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GTGGAAGCCA AGTCCCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCA GTGGAGGCCA AGTCGCCAGA GAAGGCCAAG 
TCTCCTGTGA AGGAGGAGGC CAAGTCCCCA GAGAAGGCCA AGTCTCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT GTGAAAGAGG AGGCCAAGTC CCCAGAGAAG 
GCCAAGTCTC CTGCGAAGGA GGAGGCCAAG TCCCCAGAGA AGGCCAAGTC CCCAGAGAAG 
CCCAAAAGCC CTGTGAAGGA GGAGGCCAAG TCCCCAGAGA AGCCCAAAAG CCCTGTGAAG 
GAGGAGGCCA AGTCCCCAGA GAAGCCCAAA AGCCCTGTCA AGGAGGAGGC CAAATCCCCA 
GAGAAGGCCA AGTCTCCTGT GAAGGATGAG GCCAAGGCTC CTGGGAAGGA GGTCCCAAAG 
AAGGAGGAGG CCAAGTCCCC TGTGAAGGAG GAAGAAAAAC CCCAGGAGGT GAGAGCCAAA 
GAGCCCCCAA AGAAGGCAGA GGAAGAGAAA GCTCCAGCCA TACCCAAAGC TGAGGAGAAG 
AAGGACAGCA AGAAAGATGA GGTGCCCAAG AAGGAGGCTC CAAAGCCCGA GGAGAAGAAG 
GAGCCTGCTG TGGAAAAGCC CAAAGAACCC AAAGCTGAAG CCAAGAAGGA AGAGGCTGAA 
GATAAGAAAA AAGCAACCAC CCCAGAGAAG GAGGCTCCTG CCAAGGGGAA GGAAGAGGCC 
AAGCCTAAAG AGAAGACTGA GGTGGCCAAG AAGGAACCAG ATGATGCCAA GCCCAAAGAA 
CCCAGCAAAG CAGCAGAAAA GCCAAAGAAG GAAGAGACGC CGGCAGCACC GGAGAAAAAA 
GACGCCAAGG AGGGGAAGGC CGCAGAAGCC AAGAAGCCTG AGGAGAAACC CAAAACAGAG 
GCCCAAGCCA AGGAACCTGG CAAGGAGGCC TCCACACCCG GGAAAGCCAA GGCTGAGAAA 
TCCTCCAGCA CAGACCAGAA AGACAGCAGA CCTGCAGAGA AGGCCGCAGA AGATAAGGCC 
ACCAAGGGAG AGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010821783.2
CDS	79..3036
Translation	MSFSGADALLGAFAPLHGGGSLHYALARKGGAGGARSAAGSSSGFHSWARTSVSSVSASPSRFRGAGTTSSTDSLDTLSNGPEGCVVVARSEKEQLQVLNDRFAGYIDKVRQLEAHNRSLEGEAAALRQQQAGRAAMGELYEREVREMRGAVLRLGAARGQLRLEQEHLLEDIAHVRQRLDDEARQREEAEAATRALARFAQEAEAARVELQKKAQALQEECGYLRRHHQEEVGELLGQIQSSSAAQTQAEARDALKCDVTSALREIRAQLEGHTVQSTLQSEEWFRVRLDRLSEAAKVNTDAMRSAQEEISEYRRQLQARTTELETLKSTKDSLERQRSELEDRHQADIASYQEAIQQLDAELRNTKWEMAAQLREYQDLLNVKMALDIEIAAYRKLLEGEECRIGFGPSPFLLPEGLPKIPSVSTHIKVKSEEKIKMVEKSEKETVIVEEQTEEVQVTEEVTEEEEKEAKEEEGEAVKSPPAEEAASPEKEEAKSPAEAKSPEKAKSPVKEEAKSPQKEEAKSPAEVKSPEKAKSPVEAKSPEKAKSPVKEEAKSPEKAKSPVEAKSPEKAKSPMKEEAKSPEKVKSPVEAKSPEKEEAKSPEKAKSPEKAKSPVKEEAKSPEKAKSPVEAKSPVKEEAKSPEKAKSPVEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPAKEEAKSPEKAKSPEKPKSPVKEEAKSPEKPKSPVKEEAKSPEKPKSPVKEEAKSPEKAKSPVKDEAKAPGKEVPKKEEAKSPVKEEEKPQEVRAKEPPKKAEEEKAPAIPKAEEKKDSKKDEVPKKEAPKPEEKKEPAVEKPKEPKAEAKKEEAEDKKKATTPEKEAPAKGKEEAKPKEKTEVAKKEPDDAKPKEPSKAAEKPKKEETPAAPEKKDAKEGKAAEAKKPEEKPKTEAQAKEPGKEASTPGKAKAEKSSSTDQKDSRPAEKAAEDKATKGEK

Target ORF information:

RefSeq Version	XM_010823481.2
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofilament, heavy polypeptide (NEFH), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010823481.2

ORF Insert Sequence:

ATGAGCTTCA GCGGCGCCGA CGCGCTGCTG GGCGCCTTCG CGCCGCTCCA CGGAGGCGGC 
AGCTTGCACT ATGCACTGGC TCGCAAAGGC GGCGCCGGCG GAGCGCGCTC TGCAGCCGGC 
TCCTCCAGCG GATTCCACTC ATGGGCGCGG ACCTCCGTGA GCTCCGTGTC GGCCTCCCCG 
AGCCGCTTCC GAGGCGCCGG GACTACCTCC AGCACCGACT CGCTAGACAC CCTGAGCAAC 
GGACCGGAGG GCTGCGTGGT GGTGGCGCGC AGTGAGAAGG AGCAGCTGCA GGTGCTGAAC 
GACCGCTTCG CGGGCTACAT CGACAAGGTG CGGCAGCTCG AGGCGCATAA CCGCAGCCTG 
GAGGGCGAGG CGGCGGCGCT GCGGCAGCAG CAGGCGGGCC GCGCAGCCAT GGGCGAGCTG 
TACGAGCGCG AGGTGCGCGA GATGCGCGGC GCAGTGCTGC GCTTGGGCGC GGCTCGCGGC 
CAGCTGCGCC TGGAGCAGGA GCACCTGCTG GAGGACATCG CGCACGTGCG CCAGCGCCTG 
GACGACGAGG CCCGGCAGCG CGAGGAGGCC GAGGCAGCGA CGCGCGCACT GGCCCGCTTT 
GCGCAGGAGG CCGAGGCGGC GCGCGTCGAG CTGCAGAAGA AGGCACAAGC CCTGCAGGAA 
GAGTGCGGCT ACCTGCGGCG TCACCACCAG GAGGAGGTGG GCGAGCTGCT CGGCCAGATC 
CAGAGCAGCA GCGCCGCGCA GACGCAGGCC GAGGCGCGCG ATGCCCTCAA GTGCGACGTG 
ACGTCGGCCC TGCGCGAGAT CCGCGCGCAG CTTGAAGGCC ACACGGTGCA GAGCACGCTT 
CAGTCCGAGG AGTGGTTCCG AGTGAGGCTG GACCGACTGT CCGAGGCAGC CAAGGTGAAC 
ACAGACGCCA TGCGCTCAGC ACAGGAGGAG ATATCTGAGT ACCGGCGCCA GCTGCAGGCC 
AGGACCACAG AGCTGGAGAC GCTCAAAAGC ACCAAGGACT CACTGGAGAG GCAGCGCTCT 
GAGCTGGAGG ACCGTCACCA GGCTGACATC GCATCCTACC AGGAGGCCAT CCAGCAGCTG 
GATGCAGAGC TGAGGAACAC CAAGTGGGAG ATGGCAGCCC AGCTCCGAGA GTACCAGGAT 
CTACTCAATG TCAAGATGGC TCTGGACATT GAGATCGCTG CTTACAGAAA ACTCCTGGAG 
GGTGAAGAAT GTCGAATTGG CTTTGGCCCG AGTCCTTTCC TCCTTCCAGA AGGACTCCCC 
AAGATCCCCT CCGTGTCCAC TCACATCAAA GTCAAAAGTG AAGAGAAGAT CAAGATGGTA 
GAAAAGTCAG AGAAGGAAAC TGTGATTGTG GAGGAACAGA CAGAGGAGGT CCAAGTGACT 
GAAGAAGTGA CTGAAGAAGA AGAGAAAGAG GCCAAAGAGG AGGAAGGAGA AGCAGTAAAG 
TCTCCCCCAG CAGAAGAGGC TGCATCCCCA GAGAAGGAGG AGGCCAAGTC ACCAGCCGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAGG AGGCCAAGTC CCCACAGAAG 
GAAGAGGCCA AGTCACCAGC TGAGGTGAAG TCCCCTGAGA AGGCCAAGTC CCCCGTGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAAG AAGCCAAATC CCCAGAGAAG 
GCCAAGTCCC CAGTGGAGGC CAAGTCTCCA GAAAAGGCCA AGTCCCCCAT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGT CAAATCCCCA GTGGAGGCCA AATCCCCAGA GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GAGAAAGCCA AGTCCCCTGT GAAGGAAGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA GTGGAAGCCA AGTCCCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCA GTGGAGGCCA AGTCGCCAGA GAAGGCCAAG 
TCTCCTGTGA AGGAGGAGGC CAAGTCCCCA GAGAAGGCCA AGTCTCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT GTGAAAGAGG AGGCCAAGTC CCCAGAGAAG 
GCCAAGTCTC CTGCGAAGGA GGAGGCCAAG TCCCCAGAGA AGGCCAAGTC CCCAGAGAAG 
CCCAAAAGCC CTGTGAAGGA GGAGGCCAAG TCCCCAGAGA AGCCCAAAAG CCCTGTGAAG 
GAGGAGGCCA AGTCCCCAGA GAAGCCCAAA AGCCCTGTCA AGGAGGAGGC CAAATCCCCA 
GAGAAGGCCA AGTCTCCTGT GAAGGATGAG GCCAAGGCTC CTGGGAAGGA GGTCCCAAAG 
AAGGAGGAGG CCAAGTCCCC TGTGAAGGAG GAAGAAAAAC CCCAGGAGGT GAGAGCCAAA 
GAGCCCCCAA AGAAGGCAGA GGAAGAGAAA GCTCCAGCCA TACCCAAAGC TGAGGAGAAG 
AAGGACAGCA AGAAAGATGA GGTGCCCAAG AAGGAGGCTC CAAAGCCCGA GGAGAAGAAG 
GAGCCTGCTG TGGAAAAGCC CAAAGAACCC AAAGCTGAAG CCAAGAAGGA AGAGGCTGAA 
GATAAGAAAA AAGCAACCAC CCCAGAGAAG GAGGCTCCTG CCAAGGGGAA GGAAGAGGCC 
AAGCCTAAAG AGAAGACTGA GGTGGCCAAG AAGGAACCAG ATGATGCCAA GCCCAAAGAA 
CCCAGCAAAG CAGCAGAAAA GCCAAAGAAG GAAGAGACGC CGGCAGCACC GGAGAAAAAA 
GACGCCAAGG AGGGGAAGGC CGCAGAAGCC AAGAAGCCTG AGGAGAAACC CAAAACAGAG 
GCCCAAGCCA AGGAACCTGG CAAGGAGGCC TCCACACCCG GGAAAGCCAA GGCTGAGAAA 
TCCTCCAGCA CAGACCAGAA AGACAGCAGA CCTGCAGAGA AGGCCGCAGA AGATAAGGCC 
ACCAAGGGAG AGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEFH ( XM_010823482.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010821784.2 Bos taurus neurofilament, heavy polypeptide (NEFH), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OBa51619
Clone ID Related Accession (Same CDS sequence)	XM_010823482.2 , XM_010814142.1
Accession Version	XM_010823482.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2868bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neurofilament heavy polypeptide isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646373.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010823482.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCA GCGGCGCCGA CGCGCTGCTG GGCGCCTTCG CGCCGCTCCA CGGAGGCGGC 
AGCTTGCACT ATGCACTGGC TCGCAAAGGC GGCGCCGGCG GAGCGCGCTC TGCAGCCGGC 
TCCTCCAGCG GATTCCACTC ATGGGCGCGG ACCTCCGTGA GCTCCGTGTC GGCCTCCCCG 
AGCCGCTTCC GAGGCGCCGG GACTACCTCC AGCACCGACT CGCTAGACAC CCTGAGCAAC 
GGACCGGAGG GCTGCGTGGT GGTGGCGCGC AGTGAGAAGG AGCAGCTGCA GGTGCTGAAC 
GACCGCTTCG CGGGCTACAT CGACAAGGTG CGGCAGCTCG AGGCGCATAA CCGCAGCCTG 
GAGGGCGAGG CGGCGGCGCT GCGGCAGCAG CAGGCGGGCC GCGCAGCCAT GGGCGAGCTG 
TACGAGCGCG AGGTGCGCGA GATGCGCGGC GCAGTGCTGC GCTTGGGCGC GGCTCGCGGC 
CAGCTGCGCC TGGAGCAGGA GCACCTGCTG GAGGACATCG CGCACGTGCG CCAGCGCCTG 
GACGACGAGG CCCGGCAGCG CGAGGAGGCC GAGGCAGCGA CGCGCGCACT GGCCCGCTTT 
GCGCAGGAGG CCGAGGCGGC GCGCGTCGAG CTGCAGAAGA AGGCACAAGC CCTGCAGGAA 
GAGTGCGGCT ACCTGCGGCG TCACCACCAG GAGGAGGTGG GCGAGCTGCT CGGCCAGATC 
CAGAGCAGCA GCGCCGCGCA GACGCAGGCC GAGGCGCGCG ATGCCCTCAA GTGCGACGTG 
ACGTCGGCCC TGCGCGAGAT CCGCGCGCAG CTTGAAGGCC ACACGGTGCA GAGCACGCTT 
CAGTCCGAGG AGTGGTTCCG AGTGAGGCTG GACCGACTGT CCGAGGCAGC CAAGGTGAAC 
ACAGACGCCA TGCGCTCAGC ACAGGAGGAG ATATCTGAGT ACCGGCGCCA GCTGCAGGCC 
AGGACCACAG AGCTGGAGAC GCTCAAAAGC ACCAAGGACT CACTGGAGAG GCAGCGCTCT 
GAGCTGGAGG ACCGTCACCA GGCTGACATC GCATCCTACC AGGAGGCCAT CCAGCAGCTG 
GATGCAGAGC TGAGGAACAC CAAGTGGGAG ATGGCAGCCC AGCTCCGAGA GTACCAGGAT 
CTACTCAATG TCAAGATGGC TCTGGACATT GAGATCGCTG CTTACAGAAA ACTCCTGGAG 
GGTGAAGAAT GTCGAATTGG CTTTGGCCCG AGTCCTTTCC TCCTTCCAGA AGGACTCCCC 
AAGATCCCCT CCGTGTCCAC TCACATCAAA GTCAAAAGTG AAGAGAAGAT CAAGATGGTA 
GAAAAGTCAG AGAAGGAAAC TGTGATTGTG GAGGAACAGA CAGAGGAGGT CCAAGTGACT 
GAAGAAGTGA CTGAAGAAGA AGAGAAAGAG GCCAAAGAGG AGGAAGGAGA AGCAGTAAAG 
TCTCCCCCAG CAGAAGAGGC TGCATCCCCA GAGAAGGAGG AGGCCAAGTC ACCAGCCGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAGG AGGCCAAGTC CCCAGAGAAG 
GCCAAGTCTC CAGTGGAGGC CAAGTCGCCA GAGAAGGCCA AGTCTCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT GTGAAGGAGG AGGCCAAGTC CCCAGAGAAG 
GCCAAGTCTC CTGTGAAAGA GGAGGCCAAG TCCCCAGAGA AGGCCAAGTC TCCTGTGAAG 
GAGGAGGCCA AGTCGCCAGA GAAGGCCAAG TCTCCTGTGA AAGAGGAGGC CAAGTCCCCA 
GAGAAGGCCA AGTCTCCTGT GAAAGAGGAG GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA 
GTAGAGGCCA AGTCCCCAGA GAAGGCCAAG TCTCCTGCGA AGGAGGAGGC CAAGTCGCCA 
GAGAAGGCCA AGTCTCCTGT GAAAGAGGAG GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT 
GTGAAAGAGG AGGCCAAGTC CCCAGAGAAG GCCAAGTCTC CTGCGAAGGA GGAGGCCAAG 
TCCCCAGAGA AGGCCAAGTC CCCAGAGAAG CCCAAAAGCC CTGTGAAGGA GGAGGCCAAG 
TCCCCAGAGA AGCCCAAAAG CCCTGTGAAG GAGGAGGCCA AGTCCCCAGA GAAGCCCAAA 
AGCCCTGTCA AGGAGGAGGC CAAATCCCCA GAGAAGGCCA AGTCTCCTGT GAAGGATGAG 
GCCAAGGCTC CTGGGAAGGA GGTCCCAAAG AAGGAGGAGG CCAAGTCCCC TGTGAAGGAG 
GAAGAAAAAC CCCAGGAGGT GAGAGCCAAA GAGCCCCCAA AGAAGGCAGA GGAAGAGAAA 
GCTCCAGCCA TACCCAAAGC TGAGGAGAAG AAGGACAGCA AGAAAGATGA GGTGCCCAAG 
AAGGAGGCTC CAAAGCCCGA GGAGAAGAAG GAGCCTGCTG TGGAAAAGCC CAAAGAACCC 
AAAGCTGAAG CCAAGAAGGA AGAGGCTGAA GATAAGAAAA AAGCAACCAC CCCAGAGAAG 
GAGGCTCCTG CCAAGGGGAA GGAAGAGGCC AAGCCTAAAG AGAAGACTGA GGTGGCCAAG 
AAGGAACCAG ATGATGCCAA GCCCAAAGAA CCCAGCAAAG CAGCAGAAAA GCCAAAGAAG 
GAAGAGACGC CGGCAGCACC GGAGAAAAAA GACGCCAAGG AGGGGAAGGC CGCAGAAGCC 
AAGAAGCCTG AGGAGAAACC CAAAACAGAG GCCCAAGCCA AGGAACCTGG CAAGGAGGCC 
TCCACACCCG GGAAAGCCAA GGCTGAGAAA TCCTCCAGCA CAGACCAGAA AGACAGCAGA 
CCTGCAGAGA AGGCCGCAGA AGATAAGGCC ACCAAGGGAG AGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010821784.2
CDS	79..2946
Translation	MSFSGADALLGAFAPLHGGGSLHYALARKGGAGGARSAAGSSSGFHSWARTSVSSVSASPSRFRGAGTTSSTDSLDTLSNGPEGCVVVARSEKEQLQVLNDRFAGYIDKVRQLEAHNRSLEGEAAALRQQQAGRAAMGELYEREVREMRGAVLRLGAARGQLRLEQEHLLEDIAHVRQRLDDEARQREEAEAATRALARFAQEAEAARVELQKKAQALQEECGYLRRHHQEEVGELLGQIQSSSAAQTQAEARDALKCDVTSALREIRAQLEGHTVQSTLQSEEWFRVRLDRLSEAAKVNTDAMRSAQEEISEYRRQLQARTTELETLKSTKDSLERQRSELEDRHQADIASYQEAIQQLDAELRNTKWEMAAQLREYQDLLNVKMALDIEIAAYRKLLEGEECRIGFGPSPFLLPEGLPKIPSVSTHIKVKSEEKIKMVEKSEKETVIVEEQTEEVQVTEEVTEEEEKEAKEEEGEAVKSPPAEEAASPEKEEAKSPAEAKSPEKAKSPVKEEAKSPEKAKSPVEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVEAKSPEKAKSPAKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPAKEEAKSPEKAKSPEKPKSPVKEEAKSPEKPKSPVKEEAKSPEKPKSPVKEEAKSPEKAKSPVKDEAKAPGKEVPKKEEAKSPVKEEEKPQEVRAKEPPKKAEEEKAPAIPKAEEKKDSKKDEVPKKEAPKPEEKKEPAVEKPKEPKAEAKKEEAEDKKKATTPEKEAPAKGKEEAKPKEKTEVAKKEPDDAKPKEPSKAAEKPKKEETPAAPEKKDAKEGKAAEAKKPEEKPKTEAQAKEPGKEASTPGKAKAEKSSSTDQKDSRPAEKAAEDKATKGEK

Target ORF information:

RefSeq Version	XM_010823482.2
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofilament, heavy polypeptide (NEFH), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010823482.2

ORF Insert Sequence:

ATGAGCTTCA GCGGCGCCGA CGCGCTGCTG GGCGCCTTCG CGCCGCTCCA CGGAGGCGGC 
AGCTTGCACT ATGCACTGGC TCGCAAAGGC GGCGCCGGCG GAGCGCGCTC TGCAGCCGGC 
TCCTCCAGCG GATTCCACTC ATGGGCGCGG ACCTCCGTGA GCTCCGTGTC GGCCTCCCCG 
AGCCGCTTCC GAGGCGCCGG GACTACCTCC AGCACCGACT CGCTAGACAC CCTGAGCAAC 
GGACCGGAGG GCTGCGTGGT GGTGGCGCGC AGTGAGAAGG AGCAGCTGCA GGTGCTGAAC 
GACCGCTTCG CGGGCTACAT CGACAAGGTG CGGCAGCTCG AGGCGCATAA CCGCAGCCTG 
GAGGGCGAGG CGGCGGCGCT GCGGCAGCAG CAGGCGGGCC GCGCAGCCAT GGGCGAGCTG 
TACGAGCGCG AGGTGCGCGA GATGCGCGGC GCAGTGCTGC GCTTGGGCGC GGCTCGCGGC 
CAGCTGCGCC TGGAGCAGGA GCACCTGCTG GAGGACATCG CGCACGTGCG CCAGCGCCTG 
GACGACGAGG CCCGGCAGCG CGAGGAGGCC GAGGCAGCGA CGCGCGCACT GGCCCGCTTT 
GCGCAGGAGG CCGAGGCGGC GCGCGTCGAG CTGCAGAAGA AGGCACAAGC CCTGCAGGAA 
GAGTGCGGCT ACCTGCGGCG TCACCACCAG GAGGAGGTGG GCGAGCTGCT CGGCCAGATC 
CAGAGCAGCA GCGCCGCGCA GACGCAGGCC GAGGCGCGCG ATGCCCTCAA GTGCGACGTG 
ACGTCGGCCC TGCGCGAGAT CCGCGCGCAG CTTGAAGGCC ACACGGTGCA GAGCACGCTT 
CAGTCCGAGG AGTGGTTCCG AGTGAGGCTG GACCGACTGT CCGAGGCAGC CAAGGTGAAC 
ACAGACGCCA TGCGCTCAGC ACAGGAGGAG ATATCTGAGT ACCGGCGCCA GCTGCAGGCC 
AGGACCACAG AGCTGGAGAC GCTCAAAAGC ACCAAGGACT CACTGGAGAG GCAGCGCTCT 
GAGCTGGAGG ACCGTCACCA GGCTGACATC GCATCCTACC AGGAGGCCAT CCAGCAGCTG 
GATGCAGAGC TGAGGAACAC CAAGTGGGAG ATGGCAGCCC AGCTCCGAGA GTACCAGGAT 
CTACTCAATG TCAAGATGGC TCTGGACATT GAGATCGCTG CTTACAGAAA ACTCCTGGAG 
GGTGAAGAAT GTCGAATTGG CTTTGGCCCG AGTCCTTTCC TCCTTCCAGA AGGACTCCCC 
AAGATCCCCT CCGTGTCCAC TCACATCAAA GTCAAAAGTG AAGAGAAGAT CAAGATGGTA 
GAAAAGTCAG AGAAGGAAAC TGTGATTGTG GAGGAACAGA CAGAGGAGGT CCAAGTGACT 
GAAGAAGTGA CTGAAGAAGA AGAGAAAGAG GCCAAAGAGG AGGAAGGAGA AGCAGTAAAG 
TCTCCCCCAG CAGAAGAGGC TGCATCCCCA GAGAAGGAGG AGGCCAAGTC ACCAGCCGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAGG AGGCCAAGTC CCCAGAGAAG 
GCCAAGTCTC CAGTGGAGGC CAAGTCGCCA GAGAAGGCCA AGTCTCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT GTGAAGGAGG AGGCCAAGTC CCCAGAGAAG 
GCCAAGTCTC CTGTGAAAGA GGAGGCCAAG TCCCCAGAGA AGGCCAAGTC TCCTGTGAAG 
GAGGAGGCCA AGTCGCCAGA GAAGGCCAAG TCTCCTGTGA AAGAGGAGGC CAAGTCCCCA 
GAGAAGGCCA AGTCTCCTGT GAAAGAGGAG GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA 
GTAGAGGCCA AGTCCCCAGA GAAGGCCAAG TCTCCTGCGA AGGAGGAGGC CAAGTCGCCA 
GAGAAGGCCA AGTCTCCTGT GAAAGAGGAG GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT 
GTGAAAGAGG AGGCCAAGTC CCCAGAGAAG GCCAAGTCTC CTGCGAAGGA GGAGGCCAAG 
TCCCCAGAGA AGGCCAAGTC CCCAGAGAAG CCCAAAAGCC CTGTGAAGGA GGAGGCCAAG 
TCCCCAGAGA AGCCCAAAAG CCCTGTGAAG GAGGAGGCCA AGTCCCCAGA GAAGCCCAAA 
AGCCCTGTCA AGGAGGAGGC CAAATCCCCA GAGAAGGCCA AGTCTCCTGT GAAGGATGAG 
GCCAAGGCTC CTGGGAAGGA GGTCCCAAAG AAGGAGGAGG CCAAGTCCCC TGTGAAGGAG 
GAAGAAAAAC CCCAGGAGGT GAGAGCCAAA GAGCCCCCAA AGAAGGCAGA GGAAGAGAAA 
GCTCCAGCCA TACCCAAAGC TGAGGAGAAG AAGGACAGCA AGAAAGATGA GGTGCCCAAG 
AAGGAGGCTC CAAAGCCCGA GGAGAAGAAG GAGCCTGCTG TGGAAAAGCC CAAAGAACCC 
AAAGCTGAAG CCAAGAAGGA AGAGGCTGAA GATAAGAAAA AAGCAACCAC CCCAGAGAAG 
GAGGCTCCTG CCAAGGGGAA GGAAGAGGCC AAGCCTAAAG AGAAGACTGA GGTGGCCAAG 
AAGGAACCAG ATGATGCCAA GCCCAAAGAA CCCAGCAAAG CAGCAGAAAA GCCAAAGAAG 
GAAGAGACGC CGGCAGCACC GGAGAAAAAA GACGCCAAGG AGGGGAAGGC CGCAGAAGCC 
AAGAAGCCTG AGGAGAAACC CAAAACAGAG GCCCAAGCCA AGGAACCTGG CAAGGAGGCC 
TCCACACCCG GGAAAGCCAA GGCTGAGAAA TCCTCCAGCA CAGACCAGAA AGACAGCAGA 
CCTGCAGAGA AGGCCGCAGA AGATAAGGCC ACCAAGGGAG AGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEFH ( XM_010814142.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010812444.1 Bos taurus neurofilament, heavy polypeptide (NEFH), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OBa51619
Clone ID Related Accession (Same CDS sequence)	XM_010823482.2 , XM_010814142.1
Accession Version	XM_010814142.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2868bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neurofilament heavy polypeptide isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104458.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCA GCGGCGCCGA CGCGCTGCTG GGCGCCTTCG CGCCGCTCCA CGGAGGCGGC 
AGCTTGCACT ATGCACTGGC TCGCAAAGGC GGCGCCGGCG GAGCGCGCTC TGCAGCCGGC 
TCCTCCAGCG GATTCCACTC ATGGGCGCGG ACCTCCGTGA GCTCCGTGTC GGCCTCCCCG 
AGCCGCTTCC GAGGCGCCGG GACTACCTCC AGCACCGACT CGCTAGACAC CCTGAGCAAC 
GGACCGGAGG GCTGCGTGGT GGTGGCGCGC AGTGAGAAGG AGCAGCTGCA GGTGCTGAAC 
GACCGCTTCG CGGGCTACAT CGACAAGGTG CGGCAGCTCG AGGCGCATAA CCGCAGCCTG 
GAGGGCGAGG CGGCGGCGCT GCGGCAGCAG CAGGCGGGCC GCGCAGCCAT GGGCGAGCTG 
TACGAGCGCG AGGTGCGCGA GATGCGCGGC GCAGTGCTGC GCTTGGGCGC GGCTCGCGGC 
CAGCTGCGCC TGGAGCAGGA GCACCTGCTG GAGGACATCG CGCACGTGCG CCAGCGCCTG 
GACGACGAGG CCCGGCAGCG CGAGGAGGCC GAGGCAGCGA CGCGCGCACT GGCCCGCTTT 
GCGCAGGAGG CCGAGGCGGC GCGCGTCGAG CTGCAGAAGA AGGCACAAGC CCTGCAGGAA 
GAGTGCGGCT ACCTGCGGCG TCACCACCAG GAGGAGGTGG GCGAGCTGCT CGGCCAGATC 
CAGAGCAGCA GCGCCGCGCA GACGCAGGCC GAGGCGCGCG ATGCCCTCAA GTGCGACGTG 
ACGTCGGCCC TGCGCGAGAT CCGCGCGCAG CTTGAAGGCC ACACGGTGCA GAGCACGCTT 
CAGTCCGAGG AGTGGTTCCG AGTGAGGCTG GACCGACTGT CCGAGGCAGC CAAGGTGAAC 
ACAGACGCCA TGCGCTCAGC ACAGGAGGAG ATATCTGAGT ACCGGCGCCA GCTGCAGGCC 
AGGACCACAG AGCTGGAGAC GCTCAAAAGC ACCAAGGACT CACTGGAGAG GCAGCGCTCT 
GAGCTGGAGG ACCGTCACCA GGCTGACATC GCATCCTACC AGGAGGCCAT CCAGCAGCTG 
GATGCAGAGC TGAGGAACAC CAAGTGGGAG ATGGCAGCCC AGCTCCGAGA GTACCAGGAT 
CTACTCAATG TCAAGATGGC TCTGGACATT GAGATCGCTG CTTACAGAAA ACTCCTGGAG 
GGTGAAGAAT GTCGAATTGG CTTTGGCCCG AGTCCTTTCC TCCTTCCAGA AGGACTCCCC 
AAGATCCCCT CCGTGTCCAC TCACATCAAA GTCAAAAGTG AAGAGAAGAT CAAGATGGTA 
GAAAAGTCAG AGAAGGAAAC TGTGATTGTG GAGGAACAGA CAGAGGAGGT CCAAGTGACT 
GAAGAAGTGA CTGAAGAAGA AGAGAAAGAG GCCAAAGAGG AGGAAGGAGA AGCAGTAAAG 
TCTCCCCCAG CAGAAGAGGC TGCATCCCCA GAGAAGGAGG AGGCCAAGTC ACCAGCCGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAGG AGGCCAAGTC CCCAGAGAAG 
GCCAAGTCTC CAGTGGAGGC CAAGTCGCCA GAGAAGGCCA AGTCTCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT GTGAAGGAGG AGGCCAAGTC CCCAGAGAAG 
GCCAAGTCTC CTGTGAAAGA GGAGGCCAAG TCCCCAGAGA AGGCCAAGTC TCCTGTGAAG 
GAGGAGGCCA AGTCGCCAGA GAAGGCCAAG TCTCCTGTGA AAGAGGAGGC CAAGTCCCCA 
GAGAAGGCCA AGTCTCCTGT GAAAGAGGAG GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA 
GTAGAGGCCA AGTCCCCAGA GAAGGCCAAG TCTCCTGCGA AGGAGGAGGC CAAGTCGCCA 
GAGAAGGCCA AGTCTCCTGT GAAAGAGGAG GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT 
GTGAAAGAGG AGGCCAAGTC CCCAGAGAAG GCCAAGTCTC CTGCGAAGGA GGAGGCCAAG 
TCCCCAGAGA AGGCCAAGTC CCCAGAGAAG CCCAAAAGCC CTGTGAAGGA GGAGGCCAAG 
TCCCCAGAGA AGCCCAAAAG CCCTGTGAAG GAGGAGGCCA AGTCCCCAGA GAAGCCCAAA 
AGCCCTGTCA AGGAGGAGGC CAAATCCCCA GAGAAGGCCA AGTCTCCTGT GAAGGATGAG 
GCCAAGGCTC CTGGGAAGGA GGTCCCAAAG AAGGAGGAGG CCAAGTCCCC TGTGAAGGAG 
GAAGAAAAAC CCCAGGAGGT GAGAGCCAAA GAGCCCCCAA AGAAGGCAGA GGAAGAGAAA 
GCTCCAGCCA TACCCAAAGC TGAGGAGAAG AAGGACAGCA AGAAAGATGA GGTGCCCAAG 
AAGGAGGCTC CAAAGCCCGA GGAGAAGAAG GAGCCTGCTG TGGAAAAGCC CAAAGAACCC 
AAAGCTGAAG CCAAGAAGGA AGAGGCTGAA GATAAGAAAA AAGCAACCAC CCCAGAGAAG 
GAGGCTCCTG CCAAGGGGAA GGAAGAGGCC AAGCCTAAAG AGAAGACTGA GGTGGCCAAG 
AAGGAACCAG ATGATGCCAA GCCCAAAGAA CCCAGCAAAG CAGCAGAAAA GCCAAAGAAG 
GAAGAGACGC CGGCAGCACC GGAGAAAAAA GACGCCAAGG AGGGGAAGGC CGCAGAAGCC 
AAGAAGCCTG AGGAGAAACC CAAAACAGAG GCCCAAGCCA AGGAACCTGG CAAGGAGGCC 
TCCACACCCG GGAAAGCCAA GGCTGAGAAA TCCTCCAGCA CAGACCAGAA AGACAGCAGA 
CCTGCAGAGA AGGCCGCAGA AGATAAGGCC ACCAAGGGAG AGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010812444.1
CDS	79..2946
Translation	MSFSGADALLGAFAPLHGGGSLHYALARKGGAGGARSAAGSSSGFHSWARTSVSSVSASPSRFRGAGTTSSTDSLDTLSNGPEGCVVVARSEKEQLQVLNDRFAGYIDKVRQLEAHNRSLEGEAAALRQQQAGRAAMGELYEREVREMRGAVLRLGAARGQLRLEQEHLLEDIAHVRQRLDDEARQREEAEAATRALARFAQEAEAARVELQKKAQALQEECGYLRRHHQEEVGELLGQIQSSSAAQTQAEARDALKCDVTSALREIRAQLEGHTVQSTLQSEEWFRVRLDRLSEAAKVNTDAMRSAQEEISEYRRQLQARTTELETLKSTKDSLERQRSELEDRHQADIASYQEAIQQLDAELRNTKWEMAAQLREYQDLLNVKMALDIEIAAYRKLLEGEECRIGFGPSPFLLPEGLPKIPSVSTHIKVKSEEKIKMVEKSEKETVIVEEQTEEVQVTEEVTEEEEKEAKEEEGEAVKSPPAEEAASPEKEEAKSPAEAKSPEKAKSPVKEEAKSPEKAKSPVEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVEAKSPEKAKSPAKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPAKEEAKSPEKAKSPEKPKSPVKEEAKSPEKPKSPVKEEAKSPEKPKSPVKEEAKSPEKAKSPVKDEAKAPGKEVPKKEEAKSPVKEEEKPQEVRAKEPPKKAEEEKAPAIPKAEEKKDSKKDEVPKKEAPKPEEKKEPAVEKPKEPKAEAKKEEAEDKKKATTPEKEAPAKGKEEAKPKEKTEVAKKEPDDAKPKEPSKAAEKPKKEETPAAPEKKDAKEGKAAEAKKPEEKPKTEAQAKEPGKEASTPGKAKAEKSSSTDQKDSRPAEKAAEDKATKGEK

Target ORF information:

RefSeq Version	XM_010814142.1
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofilament, heavy polypeptide (NEFH), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010814142.1

ORF Insert Sequence:

ATGAGCTTCA GCGGCGCCGA CGCGCTGCTG GGCGCCTTCG CGCCGCTCCA CGGAGGCGGC 
AGCTTGCACT ATGCACTGGC TCGCAAAGGC GGCGCCGGCG GAGCGCGCTC TGCAGCCGGC 
TCCTCCAGCG GATTCCACTC ATGGGCGCGG ACCTCCGTGA GCTCCGTGTC GGCCTCCCCG 
AGCCGCTTCC GAGGCGCCGG GACTACCTCC AGCACCGACT CGCTAGACAC CCTGAGCAAC 
GGACCGGAGG GCTGCGTGGT GGTGGCGCGC AGTGAGAAGG AGCAGCTGCA GGTGCTGAAC 
GACCGCTTCG CGGGCTACAT CGACAAGGTG CGGCAGCTCG AGGCGCATAA CCGCAGCCTG 
GAGGGCGAGG CGGCGGCGCT GCGGCAGCAG CAGGCGGGCC GCGCAGCCAT GGGCGAGCTG 
TACGAGCGCG AGGTGCGCGA GATGCGCGGC GCAGTGCTGC GCTTGGGCGC GGCTCGCGGC 
CAGCTGCGCC TGGAGCAGGA GCACCTGCTG GAGGACATCG CGCACGTGCG CCAGCGCCTG 
GACGACGAGG CCCGGCAGCG CGAGGAGGCC GAGGCAGCGA CGCGCGCACT GGCCCGCTTT 
GCGCAGGAGG CCGAGGCGGC GCGCGTCGAG CTGCAGAAGA AGGCACAAGC CCTGCAGGAA 
GAGTGCGGCT ACCTGCGGCG TCACCACCAG GAGGAGGTGG GCGAGCTGCT CGGCCAGATC 
CAGAGCAGCA GCGCCGCGCA GACGCAGGCC GAGGCGCGCG ATGCCCTCAA GTGCGACGTG 
ACGTCGGCCC TGCGCGAGAT CCGCGCGCAG CTTGAAGGCC ACACGGTGCA GAGCACGCTT 
CAGTCCGAGG AGTGGTTCCG AGTGAGGCTG GACCGACTGT CCGAGGCAGC CAAGGTGAAC 
ACAGACGCCA TGCGCTCAGC ACAGGAGGAG ATATCTGAGT ACCGGCGCCA GCTGCAGGCC 
AGGACCACAG AGCTGGAGAC GCTCAAAAGC ACCAAGGACT CACTGGAGAG GCAGCGCTCT 
GAGCTGGAGG ACCGTCACCA GGCTGACATC GCATCCTACC AGGAGGCCAT CCAGCAGCTG 
GATGCAGAGC TGAGGAACAC CAAGTGGGAG ATGGCAGCCC AGCTCCGAGA GTACCAGGAT 
CTACTCAATG TCAAGATGGC TCTGGACATT GAGATCGCTG CTTACAGAAA ACTCCTGGAG 
GGTGAAGAAT GTCGAATTGG CTTTGGCCCG AGTCCTTTCC TCCTTCCAGA AGGACTCCCC 
AAGATCCCCT CCGTGTCCAC TCACATCAAA GTCAAAAGTG AAGAGAAGAT CAAGATGGTA 
GAAAAGTCAG AGAAGGAAAC TGTGATTGTG GAGGAACAGA CAGAGGAGGT CCAAGTGACT 
GAAGAAGTGA CTGAAGAAGA AGAGAAAGAG GCCAAAGAGG AGGAAGGAGA AGCAGTAAAG 
TCTCCCCCAG CAGAAGAGGC TGCATCCCCA GAGAAGGAGG AGGCCAAGTC ACCAGCCGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCCCCT GTGAAGGAGG AGGCCAAGTC CCCAGAGAAG 
GCCAAGTCTC CAGTGGAGGC CAAGTCGCCA GAGAAGGCCA AGTCTCCTGT GAAGGAGGAG 
GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT GTGAAGGAGG AGGCCAAGTC CCCAGAGAAG 
GCCAAGTCTC CTGTGAAAGA GGAGGCCAAG TCCCCAGAGA AGGCCAAGTC TCCTGTGAAG 
GAGGAGGCCA AGTCGCCAGA GAAGGCCAAG TCTCCTGTGA AAGAGGAGGC CAAGTCCCCA 
GAGAAGGCCA AGTCTCCTGT GAAAGAGGAG GCCAAGTCCC CAGAGAAGGC CAAGTCCCCA 
GTAGAGGCCA AGTCCCCAGA GAAGGCCAAG TCTCCTGCGA AGGAGGAGGC CAAGTCGCCA 
GAGAAGGCCA AGTCTCCTGT GAAAGAGGAG GCCAAGTCCC CAGAGAAGGC CAAGTCTCCT 
GTGAAAGAGG AGGCCAAGTC CCCAGAGAAG GCCAAGTCTC CTGCGAAGGA GGAGGCCAAG 
TCCCCAGAGA AGGCCAAGTC CCCAGAGAAG CCCAAAAGCC CTGTGAAGGA GGAGGCCAAG 
TCCCCAGAGA AGCCCAAAAG CCCTGTGAAG GAGGAGGCCA AGTCCCCAGA GAAGCCCAAA 
AGCCCTGTCA AGGAGGAGGC CAAATCCCCA GAGAAGGCCA AGTCTCCTGT GAAGGATGAG 
GCCAAGGCTC CTGGGAAGGA GGTCCCAAAG AAGGAGGAGG CCAAGTCCCC TGTGAAGGAG 
GAAGAAAAAC CCCAGGAGGT GAGAGCCAAA GAGCCCCCAA AGAAGGCAGA GGAAGAGAAA 
GCTCCAGCCA TACCCAAAGC TGAGGAGAAG AAGGACAGCA AGAAAGATGA GGTGCCCAAG 
AAGGAGGCTC CAAAGCCCGA GGAGAAGAAG GAGCCTGCTG TGGAAAAGCC CAAAGAACCC 
AAAGCTGAAG CCAAGAAGGA AGAGGCTGAA GATAAGAAAA AAGCAACCAC CCCAGAGAAG 
GAGGCTCCTG CCAAGGGGAA GGAAGAGGCC AAGCCTAAAG AGAAGACTGA GGTGGCCAAG 
AAGGAACCAG ATGATGCCAA GCCCAAAGAA CCCAGCAAAG CAGCAGAAAA GCCAAAGAAG 
GAAGAGACGC CGGCAGCACC GGAGAAAAAA GACGCCAAGG AGGGGAAGGC CGCAGAAGCC 
AAGAAGCCTG AGGAGAAACC CAAAACAGAG GCCCAAGCCA AGGAACCTGG CAAGGAGGCC 
TCCACACCCG GGAAAGCCAA GGCTGAGAAA TCCTCCAGCA CAGACCAGAA AGACAGCAGA 
CCTGCAGAGA AGGCCGCAGA AGATAAGGCC ACCAAGGGAG AGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	NEFH
Entrez Gene ID	528842
Full Name	neurofilament heavy
General protein information	Preferred Names neurofilament heavy Names neurofilament heavy polypeptide neurofilament, heavy polypeptide 200kDa
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Homo sapiens (human)	NEFH	NP_066554.2
Mus musculus (house mouse)	Nefh	NP_035034.2
Rattus norvegicus (Norway rat)	Nefh	NP_036739.2
Pan troglodytes (chimpanzee)	NEFH	XP_515058.3
Canis lupus familiaris (dog)	NEFH	NP_001003352.1
Bos taurus (cattle)	NEFH	XP_005218170.1
Gallus gallus (chicken)	NEFH	XP_415310.4

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

NEFH cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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