Nefh cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nefh
Entrez Gene ID	380684
Full Name	neurofilament, heavy polypeptide
Synonyms	NF-H,NF200,Nfh,mKIAA0845
General protein information	Preferred Names neurofilament, heavy polypeptide Names neurofilament heavy polypeptide 200 kDa neurofilament protein neurofilament 200kDa neurofilament triplet H protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11 A1\|11 3.12 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_010904.3	NP_035034.2	neurofilament heavy polypeptide

Pathways from BioSystems

Homologies

Homo sapiens (human)	NEFH	NP_066554.2
Mus musculus (house mouse)	Nefh	NP_035034.2
Rattus norvegicus (Norway rat)	Nefh	NP_036739.2
Pan troglodytes (chimpanzee)	NEFH	XP_515058.3
Canis lupus familiaris (dog)	NEFH	NP_001003352.1
Bos taurus (cattle)	NEFH	XP_005218170.1
Gallus gallus (chicken)	NEFH	XP_415310.4

Gene Ontology

The following Nefh gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nefh cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu07130	NM_010904.3 Latest version!	Mus musculus neurofilament, heavy polypeptide (Nefh), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nefh ( NM_010904.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035034.2 Mus musculus neurofilament, heavy polypeptide (Nefh), mRNA.

Price & Availability↑

CloneID	OMu07130
Clone ID Related Accession (Same CDS sequence)	NM_010904.3
Accession Version	NM_010904.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	neurofilament heavy polypeptide
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL645522.12, AK122388.1, CO426942.1 and BQ174087.1. On Jan 30, 2007 this sequence version replaced NM_010904.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660817.337918.1, SRR1660815.395888.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164139 [ECO:0000348] ##Evidence-Data-END##

ATGATGAGCT TCGGCAGCGC CGATGCGCTG CTGGGCGCCC CGTTCGCGCC GCTGCACGGA 
GGCGGCAGCC TGCACTACTC GCTGAGCCGC AAGGCAGGCC CGGGCGGCAC GCGCTCCGCG 
GCCGGCTCCT CCAGCGGCTT CCACTCGTGG GCGCGGACGT CCGTGAGCTC CGTGTCCGCC 
TCACCCAGCC GCTTCCGCGG CGCCGCCTCG AGCACCGACT CGCTAGACAC CCTAAGCAAC 
GGCCCAGAGG GCTGCGTGGT GGCGGCGGTG GCGGCGCGCA GCGAGAAGGA GCAGCTGCAG 
GCTCTGAACG ACCGCTTCGC GGGCTACATC GACAAGGTGA GGCAGCTCGA GGCGCACAAC 
CGCAGCCTGG AGGGCGAGGC GGCGGCGCTG CGGCAGCAGC AAGCCGGCCG CGCCGCCATG 
GGCGAGCTGT ACGAGCGCGA GGTGCGCGAG ATGCGCGGCG CCGTGCTGCG CCTCGGGGCG 
GCGCGCGGGC AGCTGCGCCT GGAGCAGGAG CACCTGCTGG AGGACATCGC TCACGTCCGC 
CAGCGGCTGG ACGAGGAGGC CCGGCAGCGT GAGGAGGCGG AGGCGGCGGC GCGCGCCCTG 
GCGCGCTTCG CGCAGGAGGC GGAAGCGGCG CGCGTGGAGC TGCAGAAGAA GGCGCAGGCG 
CTGCAGGAGG AGTGCGGCTA CCTGCGGCGC CACCACCAGG AGGAGGTGGG CGAGCTGCTC 
GGTCAGATCC AGGGCTGCGG GGCCGCGCAG GCGCAGGCTC AGGCCGAGGC TCGCGACGCC 
CTCAAGTGCG ACGTGACGTC GGCGCTGCGG GAGATCCGCG CGCAGCTCGA AGGCCACGCG 
GTGCAGAGCA CGCTGCAGTC CGAGGAGTGG TTCCGAGTGA GGTTGGACCG ACTCTCAGAG 
GCAGCCAAAG TGAACACAGA TGCTATGCGC TCGGCCCAAG AGGAGATAAC TGAGTACCGG 
CGGCAGCTGC AAGCCAGGAC CACAGAGTTG GAGGCCCTGA AAAGCACCAA GGAGTCACTG 
GAGAGGCAGC GCTCTGAGCT AGAGGACCGT CATCAGGCAG ACATTGCCTC CTACCAGGAC 
GCTATTCAGC AGCTGGACAG TGAGCTGAGA AACACCAAGT GGGAGATGGC TGCACAGCTC 
CGAGAGTACC AGGACCTGCT CAACGTCAAG ATGGCCCTGG ACATTGAGAT TGCCGCTTAC 
AGAAAGCTCC TGGAAGGCGA AGAGTGTCGG ATTGGCTTTG GTCCGAGTCC CTTCTCTCTT 
ACTGAAGGAC TCCCAAAAAT TCCCTCCATA TCCACGCACA TAAAAGTCAA AAGCGAAGAG 
ATGATAAAGG TAGTAGAGAA ATCCGAGAAG GAAACTGTGA TTGTAGAAGG ACAGACAGAA 
GAGATCCGGG TGACGGAAGG AGTGACAGAA GAGGAGGACA AAGAGGCCCA AGGTCAGGAA 
GGAGAAGAAG CAGAAGAGGG AGAAGAAAAA GAAGAAGAGG AAGGAGCAGC AGCTACATCT 
CCCCCTGCAG AAGAGGCTGC ATCTCCAGAA AAAGAAACCA AGTCTCGTGT GAAAGAAGAG 
GCCAAGTCCC CAGGTGAGGC CAAGTCCCCA GGTGAGGCCA AGTCCCCAGC TGAGGCCAAG 
TCCCCAGGTG AGGCCAAGTC CCCAGGTGAG GCCAAGTCCC CAGGTGAGGC CAAGTCTCCA 
GCTGAGCCCA AGTCTCCAGC TGAGCCCAAG TCTCCAGCTG AGGCCAAGTC ACCAGCTGAG 
CCCAAGTCTC CAGCTACAGT GAAGTCTCCA GGTGAGGCCA AGTCACCATC TGAGGCCAAA 
TCTCCAGCTG AAGCCAAATC TCCAGCTGAG GCCAAATCTC CAGCTGAGGC CAAATCTCCA 
GCTGAGGCCA AGTCACCAGC TGAAGCCAAG TCACCAGCTG AAGCCAAATC TCCAGCTACA 
GTGAAGTCTC CAGGTGAGGC CAAGTCACCA TCTGAGGCCA AATCTCCAGC TGAAGCCAAA 
TCTCCAGCTG AGGCCAAATC TCCAGCTGAG GCCAAATCTC CAGCTGAGGT CAAGTCACCA 
GGTGAGGCCA AGTCTCCAGC TGAGCCCAAG TCACCAGCTG AGGCCAAATC TCCAGCTGAG 
GTCAAGTCAC CAGCTGAGGC CAAATCTCCA GCTGAGGTCA AGTCCCCAGG TGAGGCCAAG 
TCTCCAGCTG CAGTGAAGTC ACCAGCTGAG GCCAAGTCTC CAGCTGCAGT CAAGTCCCCA 
GGTGAGGCCA AGTCCCCAGG TGAGGCCAAG TCACCAGCTG AGGCCAAATC TCCAGCTGAG 
GCCAAGTCAC CAATTGAGGT AAAATCTCCA GAGAAGGCCA AGACCCCCGT CAAGGAAGGA 
GCAAAATCTC CAGCTGAGGC CAAGTCTCCT GAGAAGGCCA AGTCCCCCGT GAAGGAAGAT 
ATCAAGCCCC CAGCTGAGGC GAAATCCCCT GAGAAGGCCA AGAGCCCCGT GAAGGAAGGA 
GCAAAGCCTC CTGAGAAGGC CAAGCCTCTA GATGTGAAGT CTCCGGAAGC CCAGACTCCA 
GTACAGGAGG AAGCGAAGCA CCCCACAGAC ATCAGACCCC CTGAGCAGGT GAAAAGTCCT 
GCCAAGGAGA AGGCCAAGTC CCCTGAGAAG GAAGAAGCCA AGACTTCTGA AAAGGTGGCT 
CCCAAGAAGG AAGAGGTGAA GTCCCCTGTG AAGGAGGAGG TAAAAGCCAA AGAACCCCCA 
AAGAAGGTAG AAGAAGAGAA GACACTGCCT ACACCAAAGA CAGAGGCGAA GGAGAGTAAG 
AAAGACGAAG CTCCCAAGGA GGCCCCGAAG CCCAAGGTGG AGGAGAAGAA GGAAACTCCC 
ACGGAAAAGC CCAAGGACTC TACAGCAGAA GCCAAGAAGG AAGAGGCTGG AGAGAAGAAG 
AAAGCCGTGG CCTCAGAGGA GGAGACTCCT GCCAAGTTGG GTGTGAAGGA AGAAGCTAAA 
CCCAAAGAGA AGACAGAGAC AACCAAGACA GAAGCAGAAG ACACCAAGGC CAAAGAACCT 
AGCAAACCCA CAGAGACGGA AAAGCCAAAG AAAGAGGAGA TGCCAGCGGC ACCAGAGAAG 
AAAGACACCA AGGAGGAGAA GACCACAGAG TCCAGGAAGC CTGAGGAGAA GCCCAAAATG 
GAGGCCAAGG TCAAGGAGGA TGACAAGAGC CTTTCCAAAG AGCCTAGCAA ACCCAAGACA 
GAAAAGGCTG AAAAATCCTC TAGCACAGAC CAGAAAGAAA GCCAGCCCCC AGAGAAGACC 
ACAGAGGACA AGGCCACCAA GGGAGAGAAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035034.2
CDS	131..3403
Translation	MMSFGSADALLGAPFAPLHGGGSLHYSLSRKAGPGGTRSAAGSSSGFHSWARTSVSSVSASPSRFRGAASSTDSLDTLSNGPEGCVVAAVAARSEKEQLQALNDRFAGYIDKVRQLEAHNRSLEGEAAALRQQQAGRAAMGELYEREVREMRGAVLRLGAARGQLRLEQEHLLEDIAHVRQRLDEEARQREEAEAAARALARFAQEAEAARVELQKKAQALQEECGYLRRHHQEEVGELLGQIQGCGAAQAQAQAEARDALKCDVTSALREIRAQLEGHAVQSTLQSEEWFRVRLDRLSEAAKVNTDAMRSAQEEITEYRRQLQARTTELEALKSTKESLERQRSELEDRHQADIASYQDAIQQLDSELRNTKWEMAAQLREYQDLLNVKMALDIEIAAYRKLLEGEECRIGFGPSPFSLTEGLPKIPSISTHIKVKSEEMIKVVEKSEKETVIVEGQTEEIRVTEGVTEEEDKEAQGQEGEEAEEGEEKEEEEGAAATSPPAEEAASPEKETKSRVKEEAKSPGEAKSPGEAKSPAEAKSPGEAKSPGEAKSPGEAKSPAEPKSPAEPKSPAEAKSPAEPKSPATVKSPGEAKSPSEAKSPAEAKSPAEAKSPAEAKSPAEAKSPAEAKSPAEAKSPATVKSPGEAKSPSEAKSPAEAKSPAEAKSPAEAKSPAEVKSPGEAKSPAEPKSPAEAKSPAEVKSPAEAKSPAEVKSPGEAKSPAAVKSPAEAKSPAAVKSPGEAKSPGEAKSPAEAKSPAEAKSPIEVKSPEKAKTPVKEGAKSPAEAKSPEKAKSPVKEDIKPPAEAKSPEKAKSPVKEGAKPPEKAKPLDVKSPEAQTPVQEEAKHPTDIRPPEQVKSPAKEKAKSPEKEEAKTSEKVAPKKEEVKSPVKEEVKAKEPPKKVEEEKTLPTPKTEAKESKKDEAPKEAPKPKVEEKKETPTEKPKDSTAEAKKEEAGEKKKAVASEEETPAKLGVKEEAKPKEKTETTKTEAEDTKAKEPSKPTETEKPKKEEMPAAPEKKDTKEEKTTESRKPEEKPKMEAKVKEDDKSLSKEPSKPKTEKAEKSSSTDQKESQPPEKTTEDKATKGEK

Target ORF information:

RefSeq Version	NM_010904.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofilament, heavy polypeptide (Nefh), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010904.3

ORF Insert Sequence:

ATGATGAGCT TCGGCAGCGC CGATGCGCTG CTGGGCGCCC CGTTCGCGCC GCTGCACGGA 
GGCGGCAGCC TGCACTACTC GCTGAGCCGC AAGGCAGGCC CGGGCGGCAC GCGCTCCGCG 
GCCGGCTCCT CCAGCGGCTT CCACTCGTGG GCGCGGACGT CCGTGAGCTC CGTGTCCGCC 
TCACCCAGCC GCTTCCGCGG CGCCGCCTCG AGCACCGACT CGCTAGACAC CCTAAGCAAC 
GGCCCAGAGG GCTGCGTGGT GGCGGCGGTG GCGGCGCGCA GCGAGAAGGA GCAGCTGCAG 
GCTCTGAACG ACCGCTTCGC GGGCTACATC GACAAGGTGA GGCAGCTCGA GGCGCACAAC 
CGCAGCCTGG AGGGCGAGGC GGCGGCGCTG CGGCAGCAGC AAGCCGGCCG CGCCGCCATG 
GGCGAGCTGT ACGAGCGCGA GGTGCGCGAG ATGCGCGGCG CCGTGCTGCG CCTCGGGGCG 
GCGCGCGGGC AGCTGCGCCT GGAGCAGGAG CACCTGCTGG AGGACATCGC TCACGTCCGC 
CAGCGGCTGG ACGAGGAGGC CCGGCAGCGT GAGGAGGCGG AGGCGGCGGC GCGCGCCCTG 
GCGCGCTTCG CGCAGGAGGC GGAAGCGGCG CGCGTGGAGC TGCAGAAGAA GGCGCAGGCG 
CTGCAGGAGG AGTGCGGCTA CCTGCGGCGC CACCACCAGG AGGAGGTGGG CGAGCTGCTC 
GGTCAGATCC AGGGCTGCGG GGCCGCGCAG GCGCAGGCTC AGGCCGAGGC TCGCGACGCC 
CTCAAGTGCG ACGTGACGTC GGCGCTGCGG GAGATCCGCG CGCAGCTCGA AGGCCACGCG 
GTGCAGAGCA CGCTGCAGTC CGAGGAGTGG TTCCGAGTGA GGTTGGACCG ACTCTCAGAG 
GCAGCCAAAG TGAACACAGA TGCTATGCGC TCGGCCCAAG AGGAGATAAC TGAGTACCGG 
CGGCAGCTGC AAGCCAGGAC CACAGAGTTG GAGGCCCTGA AAAGCACCAA GGAGTCACTG 
GAGAGGCAGC GCTCTGAGCT AGAGGACCGT CATCAGGCAG ACATTGCCTC CTACCAGGAC 
GCTATTCAGC AGCTGGACAG TGAGCTGAGA AACACCAAGT GGGAGATGGC TGCACAGCTC 
CGAGAGTACC AGGACCTGCT CAACGTCAAG ATGGCCCTGG ACATTGAGAT TGCCGCTTAC 
AGAAAGCTCC TGGAAGGCGA AGAGTGTCGG ATTGGCTTTG GTCCGAGTCC CTTCTCTCTT 
ACTGAAGGAC TCCCAAAAAT TCCCTCCATA TCCACGCACA TAAAAGTCAA AAGCGAAGAG 
ATGATAAAGG TAGTAGAGAA ATCCGAGAAG GAAACTGTGA TTGTAGAAGG ACAGACAGAA 
GAGATCCGGG TGACGGAAGG AGTGACAGAA GAGGAGGACA AAGAGGCCCA AGGTCAGGAA 
GGAGAAGAAG CAGAAGAGGG AGAAGAAAAA GAAGAAGAGG AAGGAGCAGC AGCTACATCT 
CCCCCTGCAG AAGAGGCTGC ATCTCCAGAA AAAGAAACCA AGTCTCGTGT GAAAGAAGAG 
GCCAAGTCCC CAGGTGAGGC CAAGTCCCCA GGTGAGGCCA AGTCCCCAGC TGAGGCCAAG 
TCCCCAGGTG AGGCCAAGTC CCCAGGTGAG GCCAAGTCCC CAGGTGAGGC CAAGTCTCCA 
GCTGAGCCCA AGTCTCCAGC TGAGCCCAAG TCTCCAGCTG AGGCCAAGTC ACCAGCTGAG 
CCCAAGTCTC CAGCTACAGT GAAGTCTCCA GGTGAGGCCA AGTCACCATC TGAGGCCAAA 
TCTCCAGCTG AAGCCAAATC TCCAGCTGAG GCCAAATCTC CAGCTGAGGC CAAATCTCCA 
GCTGAGGCCA AGTCACCAGC TGAAGCCAAG TCACCAGCTG AAGCCAAATC TCCAGCTACA 
GTGAAGTCTC CAGGTGAGGC CAAGTCACCA TCTGAGGCCA AATCTCCAGC TGAAGCCAAA 
TCTCCAGCTG AGGCCAAATC TCCAGCTGAG GCCAAATCTC CAGCTGAGGT CAAGTCACCA 
GGTGAGGCCA AGTCTCCAGC TGAGCCCAAG TCACCAGCTG AGGCCAAATC TCCAGCTGAG 
GTCAAGTCAC CAGCTGAGGC CAAATCTCCA GCTGAGGTCA AGTCCCCAGG TGAGGCCAAG 
TCTCCAGCTG CAGTGAAGTC ACCAGCTGAG GCCAAGTCTC CAGCTGCAGT CAAGTCCCCA 
GGTGAGGCCA AGTCCCCAGG TGAGGCCAAG TCACCAGCTG AGGCCAAATC TCCAGCTGAG 
GCCAAGTCAC CAATTGAGGT AAAATCTCCA GAGAAGGCCA AGACCCCCGT CAAGGAAGGA 
GCAAAATCTC CAGCTGAGGC CAAGTCTCCT GAGAAGGCCA AGTCCCCCGT GAAGGAAGAT 
ATCAAGCCCC CAGCTGAGGC GAAATCCCCT GAGAAGGCCA AGAGCCCCGT GAAGGAAGGA 
GCAAAGCCTC CTGAGAAGGC CAAGCCTCTA GATGTGAAGT CTCCGGAAGC CCAGACTCCA 
GTACAGGAGG AAGCGAAGCA CCCCACAGAC ATCAGACCCC CTGAGCAGGT GAAAAGTCCT 
GCCAAGGAGA AGGCCAAGTC CCCTGAGAAG GAAGAAGCCA AGACTTCTGA AAAGGTGGCT 
CCCAAGAAGG AAGAGGTGAA GTCCCCTGTG AAGGAGGAGG TAAAAGCCAA AGAACCCCCA 
AAGAAGGTAG AAGAAGAGAA GACACTGCCT ACACCAAAGA CAGAGGCGAA GGAGAGTAAG 
AAAGACGAAG CTCCCAAGGA GGCCCCGAAG CCCAAGGTGG AGGAGAAGAA GGAAACTCCC 
ACGGAAAAGC CCAAGGACTC TACAGCAGAA GCCAAGAAGG AAGAGGCTGG AGAGAAGAAG 
AAAGCCGTGG CCTCAGAGGA GGAGACTCCT GCCAAGTTGG GTGTGAAGGA AGAAGCTAAA 
CCCAAAGAGA AGACAGAGAC AACCAAGACA GAAGCAGAAG ACACCAAGGC CAAAGAACCT 
AGCAAACCCA CAGAGACGGA AAAGCCAAAG AAAGAGGAGA TGCCAGCGGC ACCAGAGAAG 
AAAGACACCA AGGAGGAGAA GACCACAGAG TCCAGGAAGC CTGAGGAGAA GCCCAAAATG 
GAGGCCAAGG TCAAGGAGGA TGACAAGAGC CTTTCCAAAG AGCCTAGCAA ACCCAAGACA 
GAAAAGGCTG AAAAATCCTC TAGCACAGAC CAGAAAGAAA GCCAGCCCCC AGAGAAGACC 
ACAGAGGACA AGGCCACCAA GGGAGAGAAG TAA