NEFH cDNA ORF clone, Pan troglodytes(chimpanzee)

Gene
Clones
gRNAs

Summary

Gene Symbol	NEFH
Entrez Gene ID	458741
Full Name	neurofilament heavy
General protein information	Preferred Names neurofilament heavy Names neurofilament heavy polypeptide neurofilament, heavy polypeptide 200kDa
Gene Type	protein-coding
Organism	Pan troglodytes(chimpanzee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_016938911.2	XP_016794400.2	neurofilament heavy polypeptide
XM_016938911.1	XP_016794400.1	neurofilament heavy polypeptide

Pathways from BioSystems

Homologies

Homo sapiens (human)	NEFH	NP_066554.2
Mus musculus (house mouse)	Nefh	NP_035034.2
Rattus norvegicus (Norway rat)	Nefh	NP_036739.2
Pan troglodytes (chimpanzee)	NEFH	XP_515058.3
Canis lupus familiaris (dog)	NEFH	NP_001003352.1
Bos taurus (cattle)	NEFH	XP_005218170.1
Gallus gallus (chicken)	NEFH	XP_415310.4

Gene Ontology

The following NEFH gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NEFH cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPc102731	XM_016938911.2 Latest version!	Pan troglodytes neurofilament heavy (NEFH), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OPc77889	XM_016938911.1 Latest version!	Pan troglodytes neurofilament, heavy polypeptide (NEFH), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NEFH ( XM_016938911.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016794400.2 Pan troglodytes neurofilament heavy (NEFH), mRNA.

Price & Availability↑

CloneID	OPc102731
Clone ID Related Accession (Same CDS sequence)	XM_016938911.2
Accession Version	XM_016938911.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3087bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	neurofilament heavy polypeptide
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036901.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016938911.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAGCT TCGGCGGCGC GGACGCGCTG CTGGGCGCCC CGTTCGCGCC GCTGCATGGC 
GGCGGCAGCC TCCACTACGC GCTAGCCCGA AAGGGTGGCG CAGGCGGGAC GCGCTCCGCC 
GCCGGCTCCT CCAGCGGCTT CCACTCGTGG ACACGGACGT CCGTGAGCTC CGTGTCCGCC 
TCGCCCAGCC GCTTCCGTGG CGCAGGCGCC GCCTCAAGCA CCGACTCGCT GGACACGCTG 
AGCAACGGGC CGGAGGGCTG CATGGTGGCG GTGGCCACCT CGCGCAGTGA GAAGGAGCAG 
CTGCAGGCGC TGAACGACCG CTTCGCCGGG TACATCGACA AGGTGCGGCA GCTGGAGGCG 
CACAACCGCA GCCTGGAGGG CGAGGCTGCG GCGCTGCGGC AGCAGCAGGC GGGCCGCTCC 
GCTATGGGCG AGCTGTACGA GCGCGAGGTC CGCGAGATGC GCGGCGCGGT GCTGCGCCTG 
GGCGCGGCGC GCGGTCAGCT ACGCCTGGAG CAGGAGCACC TGCTCGAGGA CATCGCGCAC 
GTGCGCCAGC GCCTCGACGA CGAGGCCCGG CAGCGAGAGG AGGCCGAGGC GGCGGCCCGC 
GCGCTGGCGC GCTTCGCGCA GGAGGCCGAG GCGGCGCGCG TGGACCTGCA GAAGAAGGCG 
CAGGCGCTGC AGGAGGAGTG CGGCTACCTG CGGCGCCACC ACCAGGAAGA GGTGGGCGAG 
CTGCTCGGCC AGATCCAGGG CTCCGGCGCC GCGCAGGCGC AGATGCAGGC CGAGACGCGC 
GACGCCCTGA AGTGCGACGT GACGTCGGCG CTGCGCGAGA TTCGCGCGCA GCTTGAAGGC 
CACGCGGTGC AGAGCACGCT GCAGTCCGAG GAGTGGTTCC GAGTGAGGCT GGACCGACTG 
TCGGAGGCAG CCAAGGTGAA CACAGACGCT ATGCGCTCAG CGCAGGAGGA GATAACTGAG 
TACCGGCGTC AGCTGCAGGC CAGGACCACA GAGCTGGAGG CACTGAAAAG CACCAAGGAC 
TCACTGGAGA GGCAGCGCTC TGAGCTGGAG GACCGTCATC AGGCCGACAT TGCCTCCTAC 
CAGGAAGCCA TTCAGCAGCT GGACGCTGAG CTGAGGAACA CCAAGTGGGA GATGGCCGCC 
CAGCTGCGAG AATACCAGGA CCTGCTCAAT GTCAAGATGG CTCTGGATAT AGAGATAGCC 
GCTTACAGAA AACTCCTGGA AGGTGAAGAG TGTCGGATTG GCTTTGGCCC AATTCCTTTC 
TCGCTTCCAG AAGGACTCCC CAAAATTCCC TCTGTGTCCA CTCACATAAA GGTGAAAAGC 
GAAGAGAAGA TCAAAGTGGT GGAGAAGTCT GAGAAAGAAA CTGTGATTGT GGAGGAACAG 
ACAGAGGAGA CCCAAGTGAC TGAAGAAGTG ACTGAAGAAG AGGAGAAAGA GGCCAAAGAG 
GAGGAGGGCA AGGAGGAAGA AGGGGGTGAA GAAGAGGAGG CAGAAGGGGG AGAAGAAGAA 
ACAAAGTCTC CCCCAGCAGA AGAGGCTGCA TCCCCAGAGA AGGAAGCCAA GTCACCAGTA 
AAGGAAGAGG CAAAGTCACC GGCTGAGGCC AAGTCCCCAG AGAAGGAGGA AGCAAAATCC 
CCAGCTGAAG TCAAGTCCCC TGAGAAGGCC AAGTCTCCAG CAAAGGAAGA GGCAAAGTCA 
CCGCCTGAGG CCAAGTCCCC AGAGAAGGAG GAAGCAAAAT CTCCAGTTGA GGTCAAGTCC 
CCCGAGAAGG CCAAGTCCCC AGCAAAGGAA GAGGCAAAGT CACCGGCTGA GGCCAAGTCT 
CCAGAGAAGG CCAAGTCCCC AGTGAAGGAA GAAGCAAAGT CACCGGCTGA GGCCAAGTCC 
CCAGTGAAGG AAGAAGCAAA ATCTCCAGCT GAGGTCAAGT CCCCGGAAAA GGGCAAGTCT 
CCAACGAAGG AGGAAGCAAA GTCCCCTGAG AAGGCCAAGT CCCCAGAGAA GGAAGAGGCC 
AAGTCCCCTG AGAAGGCCAA GTCCCCAGTG AAGGCAGAAG CAAAGTCCCC TGAGAAGGCC 
AAGTCCCCAG TGAAGGCAGA AGCAAAGTCC CCTGAGAAGG CCAAGTCCCC AGTGAAGGCA 
GAAGCAAAGT CCCCTGAGAA GGCCAAGTCC CCAGTGAAGG CAGAAGCAAA GTCCCCTGAG 
AAGGCCAAGT CCCCAGTGAA GGAAGAAGCA AAGACCCCCG AGAAGGCCAA GTCCCCAGTG 
AAGGAAGAAG CCAAGTCCCC AGAGAAGGCC AAGTCCCCAG TGAAGGAAGA AGCCAAGTCC 
CCAGAGAAGG CCAAGACTCT TGATGTGAAG TCTCCAGAAG CCAAGACTCC AGCGAAGGAG 
GAAGCAAGGT CCCCTGCAGA CAAATCCCCT GAAAAGGCCA AAAGCCCTGT CAAGGAGGAG 
GTCAAGTCCC CAGAGAAGGC GAAATCTCCC CTGAAGGAGG ATGCCAAGGC CCCTGAGAAG 
GAGATCCCAA AAAAGGAAGA GGTGAAGTCC CCAGTGAAGG AGGAGGAGAA GCCCCAGGAG 
GTGAAAGTCA AAGAGCCCCC AAAGAAGGCG GAGGAAGAGA AAGCCCCTGC CACACCAAAA 
ACAGAGGAGA AGAAGGACAG CAAGAAAGAG GAGGCACCCA AGAAGGAGGC TCCAAAGCCC 
AAGGTGGAGG AGAAGAAGGA ACCTGCTGTC GAAAAGCCCA AAGAATCCAA AGTTGAAGCC 
AAGAAGGAAG AGGCTGAAGA TAAGAAAAAA GTCCCCACCC CAGAGAAGGA GGCTCCTGCC 
AAGGTGGAGG TGAAGGAAGA CGCTAAACCC AAAGAAAAGA CAGAGGTGGC CAAGAAGGAA 
CCAGATGATG CCAAGGCCAA GGAACCCAGC AGACCAGCAG AGAAGAAGGA GGCAGCACCG 
GAGAAAAAAG ACACCAAGGA GGAGAAGGCC AAGAAGCCTG AGGAGAAACC CAAGACAGAG 
GCCAAAGCCA AGGAAGATGA CAAGACCCTC TCAAAAGAGC CTAGCAAGCC TAAGGCAGAA 
AAGGCTGAAA AATCCTCCAG CACAGACCAA AAAGACAGCA AGCCTCCAGA GAAGGCCACA 
GAAGACAAGG CCGCCAAGGG GAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016794400.2
CDS	69..3155
Translation	MMSFGGADALLGAPFAPLHGGGSLHYALARKGGAGGTRSAAGSSSGFHSWTRTSVSSVSASPSRFRGAGAASSTDSLDTLSNGPEGCMVAVATSRSEKEQLQALNDRFAGYIDKVRQLEAHNRSLEGEAAALRQQQAGRSAMGELYEREVREMRGAVLRLGAARGQLRLEQEHLLEDIAHVRQRLDDEARQREEAEAAARALARFAQEAEAARVDLQKKAQALQEECGYLRRHHQEEVGELLGQIQGSGAAQAQMQAETRDALKCDVTSALREIRAQLEGHAVQSTLQSEEWFRVRLDRLSEAAKVNTDAMRSAQEEITEYRRQLQARTTELEALKSTKDSLERQRSELEDRHQADIASYQEAIQQLDAELRNTKWEMAAQLREYQDLLNVKMALDIEIAAYRKLLEGEECRIGFGPIPFSLPEGLPKIPSVSTHIKVKSEEKIKVVEKSEKETVIVEEQTEETQVTEEVTEEEEKEAKEEEGKEEEGGEEEEAEGGEEETKSPPAEEAASPEKEAKSPVKEEAKSPAEAKSPEKEEAKSPAEVKSPEKAKSPAKEEAKSPPEAKSPEKEEAKSPVEVKSPEKAKSPAKEEAKSPAEAKSPEKAKSPVKEEAKSPAEAKSPVKEEAKSPAEVKSPEKGKSPTKEEAKSPEKAKSPEKEEAKSPEKAKSPVKAEAKSPEKAKSPVKAEAKSPEKAKSPVKAEAKSPEKAKSPVKAEAKSPEKAKSPVKEEAKTPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKTLDVKSPEAKTPAKEEARSPADKSPEKAKSPVKEEVKSPEKAKSPLKEDAKAPEKEIPKKEEVKSPVKEEEKPQEVKVKEPPKKAEEEKAPATPKTEEKKDSKKEEAPKKEAPKPKVEEKKEPAVEKPKESKVEAKKEEAEDKKKVPTPEKEAPAKVEVKEDAKPKEKTEVAKKEPDDAKAKEPSRPAEKKEAAPEKKDTKEEKAKKPEEKPKTEAKAKEDDKTLSKEPSKPKAEKAEKSSSTDQKDSKPPEKATEDKAAKGK

Target ORF information:

RefSeq Version	XM_016938911.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes neurofilament heavy (NEFH), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016938911.2

ORF Insert Sequence:

ATGATGAGCT TCGGCGGCGC GGACGCGCTG CTGGGCGCCC CGTTCGCGCC GCTGCATGGC 
GGCGGCAGCC TCCACTACGC GCTAGCCCGA AAGGGTGGCG CAGGCGGGAC GCGCTCCGCC 
GCCGGCTCCT CCAGCGGCTT CCACTCGTGG ACACGGACGT CCGTGAGCTC CGTGTCCGCC 
TCGCCCAGCC GCTTCCGTGG CGCAGGCGCC GCCTCAAGCA CCGACTCGCT GGACACGCTG 
AGCAACGGGC CGGAGGGCTG CATGGTGGCG GTGGCCACCT CGCGCAGTGA GAAGGAGCAG 
CTGCAGGCGC TGAACGACCG CTTCGCCGGG TACATCGACA AGGTGCGGCA GCTGGAGGCG 
CACAACCGCA GCCTGGAGGG CGAGGCTGCG GCGCTGCGGC AGCAGCAGGC GGGCCGCTCC 
GCTATGGGCG AGCTGTACGA GCGCGAGGTC CGCGAGATGC GCGGCGCGGT GCTGCGCCTG 
GGCGCGGCGC GCGGTCAGCT ACGCCTGGAG CAGGAGCACC TGCTCGAGGA CATCGCGCAC 
GTGCGCCAGC GCCTCGACGA CGAGGCCCGG CAGCGAGAGG AGGCCGAGGC GGCGGCCCGC 
GCGCTGGCGC GCTTCGCGCA GGAGGCCGAG GCGGCGCGCG TGGACCTGCA GAAGAAGGCG 
CAGGCGCTGC AGGAGGAGTG CGGCTACCTG CGGCGCCACC ACCAGGAAGA GGTGGGCGAG 
CTGCTCGGCC AGATCCAGGG CTCCGGCGCC GCGCAGGCGC AGATGCAGGC CGAGACGCGC 
GACGCCCTGA AGTGCGACGT GACGTCGGCG CTGCGCGAGA TTCGCGCGCA GCTTGAAGGC 
CACGCGGTGC AGAGCACGCT GCAGTCCGAG GAGTGGTTCC GAGTGAGGCT GGACCGACTG 
TCGGAGGCAG CCAAGGTGAA CACAGACGCT ATGCGCTCAG CGCAGGAGGA GATAACTGAG 
TACCGGCGTC AGCTGCAGGC CAGGACCACA GAGCTGGAGG CACTGAAAAG CACCAAGGAC 
TCACTGGAGA GGCAGCGCTC TGAGCTGGAG GACCGTCATC AGGCCGACAT TGCCTCCTAC 
CAGGAAGCCA TTCAGCAGCT GGACGCTGAG CTGAGGAACA CCAAGTGGGA GATGGCCGCC 
CAGCTGCGAG AATACCAGGA CCTGCTCAAT GTCAAGATGG CTCTGGATAT AGAGATAGCC 
GCTTACAGAA AACTCCTGGA AGGTGAAGAG TGTCGGATTG GCTTTGGCCC AATTCCTTTC 
TCGCTTCCAG AAGGACTCCC CAAAATTCCC TCTGTGTCCA CTCACATAAA GGTGAAAAGC 
GAAGAGAAGA TCAAAGTGGT GGAGAAGTCT GAGAAAGAAA CTGTGATTGT GGAGGAACAG 
ACAGAGGAGA CCCAAGTGAC TGAAGAAGTG ACTGAAGAAG AGGAGAAAGA GGCCAAAGAG 
GAGGAGGGCA AGGAGGAAGA AGGGGGTGAA GAAGAGGAGG CAGAAGGGGG AGAAGAAGAA 
ACAAAGTCTC CCCCAGCAGA AGAGGCTGCA TCCCCAGAGA AGGAAGCCAA GTCACCAGTA 
AAGGAAGAGG CAAAGTCACC GGCTGAGGCC AAGTCCCCAG AGAAGGAGGA AGCAAAATCC 
CCAGCTGAAG TCAAGTCCCC TGAGAAGGCC AAGTCTCCAG CAAAGGAAGA GGCAAAGTCA 
CCGCCTGAGG CCAAGTCCCC AGAGAAGGAG GAAGCAAAAT CTCCAGTTGA GGTCAAGTCC 
CCCGAGAAGG CCAAGTCCCC AGCAAAGGAA GAGGCAAAGT CACCGGCTGA GGCCAAGTCT 
CCAGAGAAGG CCAAGTCCCC AGTGAAGGAA GAAGCAAAGT CACCGGCTGA GGCCAAGTCC 
CCAGTGAAGG AAGAAGCAAA ATCTCCAGCT GAGGTCAAGT CCCCGGAAAA GGGCAAGTCT 
CCAACGAAGG AGGAAGCAAA GTCCCCTGAG AAGGCCAAGT CCCCAGAGAA GGAAGAGGCC 
AAGTCCCCTG AGAAGGCCAA GTCCCCAGTG AAGGCAGAAG CAAAGTCCCC TGAGAAGGCC 
AAGTCCCCAG TGAAGGCAGA AGCAAAGTCC CCTGAGAAGG CCAAGTCCCC AGTGAAGGCA 
GAAGCAAAGT CCCCTGAGAA GGCCAAGTCC CCAGTGAAGG CAGAAGCAAA GTCCCCTGAG 
AAGGCCAAGT CCCCAGTGAA GGAAGAAGCA AAGACCCCCG AGAAGGCCAA GTCCCCAGTG 
AAGGAAGAAG CCAAGTCCCC AGAGAAGGCC AAGTCCCCAG TGAAGGAAGA AGCCAAGTCC 
CCAGAGAAGG CCAAGACTCT TGATGTGAAG TCTCCAGAAG CCAAGACTCC AGCGAAGGAG 
GAAGCAAGGT CCCCTGCAGA CAAATCCCCT GAAAAGGCCA AAAGCCCTGT CAAGGAGGAG 
GTCAAGTCCC CAGAGAAGGC GAAATCTCCC CTGAAGGAGG ATGCCAAGGC CCCTGAGAAG 
GAGATCCCAA AAAAGGAAGA GGTGAAGTCC CCAGTGAAGG AGGAGGAGAA GCCCCAGGAG 
GTGAAAGTCA AAGAGCCCCC AAAGAAGGCG GAGGAAGAGA AAGCCCCTGC CACACCAAAA 
ACAGAGGAGA AGAAGGACAG CAAGAAAGAG GAGGCACCCA AGAAGGAGGC TCCAAAGCCC 
AAGGTGGAGG AGAAGAAGGA ACCTGCTGTC GAAAAGCCCA AAGAATCCAA AGTTGAAGCC 
AAGAAGGAAG AGGCTGAAGA TAAGAAAAAA GTCCCCACCC CAGAGAAGGA GGCTCCTGCC 
AAGGTGGAGG TGAAGGAAGA CGCTAAACCC AAAGAAAAGA CAGAGGTGGC CAAGAAGGAA 
CCAGATGATG CCAAGGCCAA GGAACCCAGC AGACCAGCAG AGAAGAAGGA GGCAGCACCG 
GAGAAAAAAG ACACCAAGGA GGAGAAGGCC AAGAAGCCTG AGGAGAAACC CAAGACAGAG 
GCCAAAGCCA AGGAAGATGA CAAGACCCTC TCAAAAGAGC CTAGCAAGCC TAAGGCAGAA 
AAGGCTGAAA AATCCTCCAG CACAGACCAA AAAGACAGCA AGCCTCCAGA GAAGGCCACA 
GAAGACAAGG CCGCCAAGGG GAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEFH ( XM_016938911.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016794400.1 Pan troglodytes neurofilament, heavy polypeptide (NEFH), mRNA.

Price & Availability↑

CloneID	OPc77889
Clone ID Related Accession (Same CDS sequence)	XM_016938911.1
Accession Version	XM_016938911.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3087bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	neurofilament heavy polypeptide
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006489.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAGCT TCGGCGGCGC GGACGCGCTG CTGGGCGCCC CGTTCGCGCC GCTGCATGGC 
GGCGGCAGCC TCCACTACGC GCTAGCCCGA AAGGGTGGCG CAGGCGGGAC GCGCTCCGCC 
GCCGGCTCCT CCAGCGGCTT CCACTCGTGG ACACGGACGT CCGTGAGCTC CGTGTCCGCC 
TCGCCCAGCC GCTTCCGTGG CGCAGGCGCC GCCTCAAGCA CCGACTCGCT GGACACGCTG 
AGCAACGGGC CGGAGGGCTG CATGGTGGCG GTGGCCACCT CGCGCAGTGA GAAGGAGCAG 
CTGCAGGCGC TGAACGACCG CTTCGCCGGG TACATCGACA AGGTGCGGCA GCTGGAGGCG 
CACAACCGCA GCCTGGAGGG CGAGGCTGCG GCGCTGCGGC AGCAGCAGGC GGGCCGCTCC 
GCTATGGGCG AGCTGTACGA GCGCGAGGTC CGCGAGATGC GCGGCGCGGT GCTGCGCCTG 
GGCGCGGCGC GCGGTCAGCT ACGCCTGGAG CAGGAGCACC TGCTCGAGGA CATCGCGCAC 
GTGCGCCAGC GCCTCGACGA CGAGGCCCGG CAGCGAGAGG AGGCCGAGGC GGCGGCCCGC 
GCGCTGGCGC GCTTCGCGCA GGAGGCCGAG GCGGCGCGCG TGGACCTGCA GAAGAAGGCG 
CAGGCGCTGC AGGAGGAGTG CGGCTACCTG CGGCGCCACC ACCAGGAAGA GGTGGGCGAG 
CTGCTCGGCC AGATCCAGGG CTCCGGCGCC GCGCAGGCGC AGATGCAGGC CGAGACGCGC 
GACGCCCTGA AGTGCGACGT GACGTCGGCG CTGCGCGAGA TTCGCGCGCA GCTTGAAGGC 
CACGCGGTGC AGAGCACGCT GCAGTCCGAG GAGTGGTTCC GAGTGAGGCT GGACCGACTG 
TCGGAGGCAG CCAAGGTGAA CACAGACGCT ATGCGCTCAG CGCAGGAGGA GATAACTGAG 
TACCGGCGTC AGCTGCAGGC CAGGACCACA GAGCTGGAGG CACTGAAAAG CACCAAGGAC 
TCACTGGAGA GGCAGCGCTC TGAGCTGGAG GACCGTCATC AGGCCGACAT TGCCTCCTAC 
CAGGAAGCCA TTCAGCAGCT GGACGCTGAG CTGAGGAACA CCAAGTGGGA GATGGCCGCC 
CAGCTGCGAG AATACCAGGA CCTGCTCAAT GTCAAGATGG CTCTGGATAT AGAGATAGCC 
GCTTACAGAA AACTCCTGGA AGGTGAAGAG TGTCGGATTG GCTTTGGCCC AATTCCTTTC 
TCGCTTCCAG AAGGACTCCC CAAAATTCCC TCTGTGTCCA CTCACATAAA GGTGAAAAGC 
GAAGAGAAGA TCAAAGTGGT GGAGAAGTCT GAGAAAGAAA CTGTGATTGT GGAGGAACAG 
ACAGAGGAGA CCCAAGTGAC TGAAGAAGTG ACTGAAGAAG AGGAGAAAGA GGCCAAAGAG 
GAGGAGGGCA AGGAGGAAGA AGGGGGTGAA GAAGAGGAGG CAGAAGGGGG AGAAGAAGAA 
ACAAAGTCTC CCCCAGCAGA AGAGGCTGCA TCCCCAGAGA AGGAAGCCAA GTCACCAGTA 
AAGGAAGAGG CAAAGTCACC GGCTGAGGCC AAGTCCCCAG AGAAGGAGGA AGCAAAATCC 
CCAGCTGAAG TCAAGTCCCC TGAGAAGGCC AAGTCTCCAG CAAAGGAAGA GGCAAAGTCA 
CCGCCTGAGG CCAAGTCCCC AGAGAAGGAG GAAGCAAAAT CTCCAGTTGA GGTCAAGTCC 
CCCGAGAAGG CCAAGTCCCC AGCAAAGGAA GAGGCAAAGT CACCGGCTGA GGCCAAGTCT 
CCAGAGAAGG CCAAGTCCCC AGTGAAGGAA GAAGCAAAGT CACCGGCTGA GGCCAAGTCC 
CCAGTGAAGG AAGAAGCAAA ATCTCCAGCT GAGGTCAAGT CCCCGGAAAA GGGCAAGTCT 
CCAACGAAGG AGGAAGCAAA GTCCCCTGAG AAGGCCAAGT CCCCAGAGAA GGAAGAGGCC 
AAGTCCCCTG AGAAGGCCAA GTCCCCAGTG AAGGCAGAAG CAAAGTCCCC TGAGAAGGCC 
AAGTCCCCAG TGAAGGCAGA AGCAAAGTCC CCTGAGAAGG CCAAGTCCCC AGTGAAGGCA 
GAAGCAAAGT CCCCTGAGAA GGCCAAGTCC CCAGTGAAGG CAGAAGCAAA GTCCCCTGAG 
AAGGCCAAGT CCCCAGTGAA GGAAGAAGCA AAGACCCCCG AGAAGGCCAA GTCCCCAGTG 
AAGGAAGAAG CCAAGTCCCC AGAGAAGGCC AAGTCCCCAG TGAAGGAAGA AGCCAAGTCC 
CCAGAGAAGG CCAAGACTCT TGATGTGAAG TCTCCAGAAG CCAAGACTCC AGCGAAGGAG 
GAAGCAAGGT CCCCTGCAGA CAAATCCCCT GAAAAGGCCA AAAGCCCTGT CAAGGAGGAG 
GTCAAGTCCC CAGAGAAGGC GAAATCTCCC CTGAAGGAGG ATGCCAAGGC CCCTGAGAAG 
GAGATCCCAA AAAAGGAAGA GGTGAAGTCC CCAGTGAAGG AGGAGGAGAA GCCCCAGGAG 
GTGAAAGTCA AAGAGCCCCC AAAGAAGGCG GAGGAAGAGA AAGCCCCTGC CACACCAAAA 
ACAGAGGAGA AGAAGGACAG CAAGAAAGAG GAGGCACCCA AGAAGGAGGC TCCAAAGCCC 
AAGGTGGAGG AGAAGAAGGA ACCTGCTGTC GAAAAGCCCA AAGAATCCAA AGTTGAAGCC 
AAGAAGGAAG AGGCTGAAGA TAAGAAAAAA GTCCCCACCC CAGAGAAGGA GGCTCCTGCC 
AAGGTGGAGG TGAAGGAAGA CGCTAAACCC AAAGAAAAGA CAGAGGTGGC CAAGAAGGAA 
CCAGATGATG CCAAGGCCAA GGAACCCAGC AAACCAGCAG AGAAGAAGGA GGCAGCACCG 
GAGAAAAAAG ACACCAAGGA GGAGAAGGCC AAGAAGCCTG AGGAGAAACC CAAGACAGAG 
GCCAAAGCCA AGGAAGATGA CAAGACCCTC TCAAAAGAGC CTAGCAAGCC TAAGGCAGAA 
AAGGCTGAAA AATCCTCCAG CACAGACCAA AAAGACAGCA AGCCTCCAGA GAAGGCCACA 
GAAGACAAGG CCGCCAAGGG GAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016794400.1
CDS	69..3155
Translation	MMSFGGADALLGAPFAPLHGGGSLHYALARKGGAGGTRSAAGSSSGFHSWTRTSVSSVSASPSRFRGAGAASSTDSLDTLSNGPEGCMVAVATSRSEKEQLQALNDRFAGYIDKVRQLEAHNRSLEGEAAALRQQQAGRSAMGELYEREVREMRGAVLRLGAARGQLRLEQEHLLEDIAHVRQRLDDEARQREEAEAAARALARFAQEAEAARVDLQKKAQALQEECGYLRRHHQEEVGELLGQIQGSGAAQAQMQAETRDALKCDVTSALREIRAQLEGHAVQSTLQSEEWFRVRLDRLSEAAKVNTDAMRSAQEEITEYRRQLQARTTELEALKSTKDSLERQRSELEDRHQADIASYQEAIQQLDAELRNTKWEMAAQLREYQDLLNVKMALDIEIAAYRKLLEGEECRIGFGPIPFSLPEGLPKIPSVSTHIKVKSEEKIKVVEKSEKETVIVEEQTEETQVTEEVTEEEEKEAKEEEGKEEEGGEEEEAEGGEEETKSPPAEEAASPEKEAKSPVKEEAKSPAEAKSPEKEEAKSPAEVKSPEKAKSPAKEEAKSPPEAKSPEKEEAKSPVEVKSPEKAKSPAKEEAKSPAEAKSPEKAKSPVKEEAKSPAEAKSPVKEEAKSPAEVKSPEKGKSPTKEEAKSPEKAKSPEKEEAKSPEKAKSPVKAEAKSPEKAKSPVKAEAKSPEKAKSPVKAEAKSPEKAKSPVKAEAKSPEKAKSPVKEEAKTPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKTLDVKSPEAKTPAKEEARSPADKSPEKAKSPVKEEVKSPEKAKSPLKEDAKAPEKEIPKKEEVKSPVKEEEKPQEVKVKEPPKKAEEEKAPATPKTEEKKDSKKEEAPKKEAPKPKVEEKKEPAVEKPKESKVEAKKEEAEDKKKVPTPEKEAPAKVEVKEDAKPKEKTEVAKKEPDDAKAKEPSKPAEKKEAAPEKKDTKEEKAKKPEEKPKTEAKAKEDDKTLSKEPSKPKAEKAEKSSSTDQKDSKPPEKATEDKAAKGK

Target ORF information:

RefSeq Version	XM_016938911.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes neurofilament, heavy polypeptide (NEFH), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016938911.1

ORF Insert Sequence:

ATGATGAGCT TCGGCGGCGC GGACGCGCTG CTGGGCGCCC CGTTCGCGCC GCTGCATGGC 
GGCGGCAGCC TCCACTACGC GCTAGCCCGA AAGGGTGGCG CAGGCGGGAC GCGCTCCGCC 
GCCGGCTCCT CCAGCGGCTT CCACTCGTGG ACACGGACGT CCGTGAGCTC CGTGTCCGCC 
TCGCCCAGCC GCTTCCGTGG CGCAGGCGCC GCCTCAAGCA CCGACTCGCT GGACACGCTG 
AGCAACGGGC CGGAGGGCTG CATGGTGGCG GTGGCCACCT CGCGCAGTGA GAAGGAGCAG 
CTGCAGGCGC TGAACGACCG CTTCGCCGGG TACATCGACA AGGTGCGGCA GCTGGAGGCG 
CACAACCGCA GCCTGGAGGG CGAGGCTGCG GCGCTGCGGC AGCAGCAGGC GGGCCGCTCC 
GCTATGGGCG AGCTGTACGA GCGCGAGGTC CGCGAGATGC GCGGCGCGGT GCTGCGCCTG 
GGCGCGGCGC GCGGTCAGCT ACGCCTGGAG CAGGAGCACC TGCTCGAGGA CATCGCGCAC 
GTGCGCCAGC GCCTCGACGA CGAGGCCCGG CAGCGAGAGG AGGCCGAGGC GGCGGCCCGC 
GCGCTGGCGC GCTTCGCGCA GGAGGCCGAG GCGGCGCGCG TGGACCTGCA GAAGAAGGCG 
CAGGCGCTGC AGGAGGAGTG CGGCTACCTG CGGCGCCACC ACCAGGAAGA GGTGGGCGAG 
CTGCTCGGCC AGATCCAGGG CTCCGGCGCC GCGCAGGCGC AGATGCAGGC CGAGACGCGC 
GACGCCCTGA AGTGCGACGT GACGTCGGCG CTGCGCGAGA TTCGCGCGCA GCTTGAAGGC 
CACGCGGTGC AGAGCACGCT GCAGTCCGAG GAGTGGTTCC GAGTGAGGCT GGACCGACTG 
TCGGAGGCAG CCAAGGTGAA CACAGACGCT ATGCGCTCAG CGCAGGAGGA GATAACTGAG 
TACCGGCGTC AGCTGCAGGC CAGGACCACA GAGCTGGAGG CACTGAAAAG CACCAAGGAC 
TCACTGGAGA GGCAGCGCTC TGAGCTGGAG GACCGTCATC AGGCCGACAT TGCCTCCTAC 
CAGGAAGCCA TTCAGCAGCT GGACGCTGAG CTGAGGAACA CCAAGTGGGA GATGGCCGCC 
CAGCTGCGAG AATACCAGGA CCTGCTCAAT GTCAAGATGG CTCTGGATAT AGAGATAGCC 
GCTTACAGAA AACTCCTGGA AGGTGAAGAG TGTCGGATTG GCTTTGGCCC AATTCCTTTC 
TCGCTTCCAG AAGGACTCCC CAAAATTCCC TCTGTGTCCA CTCACATAAA GGTGAAAAGC 
GAAGAGAAGA TCAAAGTGGT GGAGAAGTCT GAGAAAGAAA CTGTGATTGT GGAGGAACAG 
ACAGAGGAGA CCCAAGTGAC TGAAGAAGTG ACTGAAGAAG AGGAGAAAGA GGCCAAAGAG 
GAGGAGGGCA AGGAGGAAGA AGGGGGTGAA GAAGAGGAGG CAGAAGGGGG AGAAGAAGAA 
ACAAAGTCTC CCCCAGCAGA AGAGGCTGCA TCCCCAGAGA AGGAAGCCAA GTCACCAGTA 
AAGGAAGAGG CAAAGTCACC GGCTGAGGCC AAGTCCCCAG AGAAGGAGGA AGCAAAATCC 
CCAGCTGAAG TCAAGTCCCC TGAGAAGGCC AAGTCTCCAG CAAAGGAAGA GGCAAAGTCA 
CCGCCTGAGG CCAAGTCCCC AGAGAAGGAG GAAGCAAAAT CTCCAGTTGA GGTCAAGTCC 
CCCGAGAAGG CCAAGTCCCC AGCAAAGGAA GAGGCAAAGT CACCGGCTGA GGCCAAGTCT 
CCAGAGAAGG CCAAGTCCCC AGTGAAGGAA GAAGCAAAGT CACCGGCTGA GGCCAAGTCC 
CCAGTGAAGG AAGAAGCAAA ATCTCCAGCT GAGGTCAAGT CCCCGGAAAA GGGCAAGTCT 
CCAACGAAGG AGGAAGCAAA GTCCCCTGAG AAGGCCAAGT CCCCAGAGAA GGAAGAGGCC 
AAGTCCCCTG AGAAGGCCAA GTCCCCAGTG AAGGCAGAAG CAAAGTCCCC TGAGAAGGCC 
AAGTCCCCAG TGAAGGCAGA AGCAAAGTCC CCTGAGAAGG CCAAGTCCCC AGTGAAGGCA 
GAAGCAAAGT CCCCTGAGAA GGCCAAGTCC CCAGTGAAGG CAGAAGCAAA GTCCCCTGAG 
AAGGCCAAGT CCCCAGTGAA GGAAGAAGCA AAGACCCCCG AGAAGGCCAA GTCCCCAGTG 
AAGGAAGAAG CCAAGTCCCC AGAGAAGGCC AAGTCCCCAG TGAAGGAAGA AGCCAAGTCC 
CCAGAGAAGG CCAAGACTCT TGATGTGAAG TCTCCAGAAG CCAAGACTCC AGCGAAGGAG 
GAAGCAAGGT CCCCTGCAGA CAAATCCCCT GAAAAGGCCA AAAGCCCTGT CAAGGAGGAG 
GTCAAGTCCC CAGAGAAGGC GAAATCTCCC CTGAAGGAGG ATGCCAAGGC CCCTGAGAAG 
GAGATCCCAA AAAAGGAAGA GGTGAAGTCC CCAGTGAAGG AGGAGGAGAA GCCCCAGGAG 
GTGAAAGTCA AAGAGCCCCC AAAGAAGGCG GAGGAAGAGA AAGCCCCTGC CACACCAAAA 
ACAGAGGAGA AGAAGGACAG CAAGAAAGAG GAGGCACCCA AGAAGGAGGC TCCAAAGCCC 
AAGGTGGAGG AGAAGAAGGA ACCTGCTGTC GAAAAGCCCA AAGAATCCAA AGTTGAAGCC 
AAGAAGGAAG AGGCTGAAGA TAAGAAAAAA GTCCCCACCC CAGAGAAGGA GGCTCCTGCC 
AAGGTGGAGG TGAAGGAAGA CGCTAAACCC AAAGAAAAGA CAGAGGTGGC CAAGAAGGAA 
CCAGATGATG CCAAGGCCAA GGAACCCAGC AAACCAGCAG AGAAGAAGGA GGCAGCACCG 
GAGAAAAAAG ACACCAAGGA GGAGAAGGCC AAGAAGCCTG AGGAGAAACC CAAGACAGAG 
GCCAAAGCCA AGGAAGATGA CAAGACCCTC TCAAAAGAGC CTAGCAAGCC TAAGGCAGAA 
AAGGCTGAAA AATCCTCCAG CACAGACCAA AAAGACAGCA AGCCTCCAGA GAAGGCCACA 
GAAGACAAGG CCGCCAAGGG GAAGTAA