PLEKHG1 cDNA ORF clone, Bos taurus(cattle)

The following PLEKHG1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PLEKHG1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa02012 NM_001205921.1
Latest version!
Bos taurus pleckstrin homology domain containing, family G (with RhoGef domain) member 1 (PLEKHG1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa237778 NM_001205921.2
Latest version!
Bos taurus pleckstrin homology and RhoGEF domain containing G1 (PLEKHG1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa237775 XM_024996651.1
Latest version!
Bos taurus pleckstrin homology and RhoGEF domain containing G1 (PLEKHG1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa237776 XM_024996652.1
Latest version!
Bos taurus pleckstrin homology and RhoGEF domain containing G1 (PLEKHG1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa237777 XM_024996653.1
Latest version!
Bos taurus pleckstrin homology and RhoGEF domain containing G1 (PLEKHG1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa237778 XM_024996656.1
Latest version!
Bos taurus pleckstrin homology and RhoGEF domain containing G1 (PLEKHG1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa237778 XM_024996655.1
Latest version!
Bos taurus pleckstrin homology and RhoGEF domain containing G1 (PLEKHG1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa237778 XM_024996654.1
Latest version!
Bos taurus pleckstrin homology and RhoGEF domain containing G1 (PLEKHG1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa237778 XM_024996658.1
Latest version!
Bos taurus pleckstrin homology and RhoGEF domain containing G1 (PLEKHG1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa02012
    Clone ID Related Accession (Same CDS sequence) NM_001205921.1
    Accession Version NM_001205921.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4143bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2012-08-31
    Organism Bos taurus(cattle)
    Product pleckstrin homology domain-containing family G member 1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03065069.1, AAFC03065070.1 and AAFC03029655.1. On Apr 20, 2011 this sequence version replaced XM_604128.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAGCTCT CCGACAGTGA CCGCCCGGTC AGCTTCGGCT CCACGTCGTC CTCGGCTTCC 
    TCCCGGGACA GCCATGGTTC CTTCGGCAGC AGAATGACCT TAGTTTCAAG CAGCCATCTG
    GGCTTGTTTC CTCAGGATAA GGAAGCAGGG GCCATAAAAC TGGAGCTGAT GCCAGCCAGG
    CCGTTTTCCA GCAGCGAGCT GCAGACGGAC CGTGCGGCCG GGCAGCAGAG CACAGACGAG
    GGCGGCGAGA GGCAGCCCCG GGCACAGCGC AGAGTGGAAG CCAACGGGCC GACCAAAACC
    GGGGCCGAAT CGGCCACCAG CCCCAAGCTG CTCTATGTGG ACAGAGTTGT GCAGGAAATT
    CTGGAGACCG AACGGACTTA CGTGCAAGAT TTAAAGAGCA TCGTAGAGGA TTACCTTGAC
    TGTATCAGGG ACCAAACGAA ACTTCCTCTG GGGACAGATG AAAGATCAGC CCTTTTTGGA
    AACATACAGG ATATCTACCA CTTTAATAGT GAACTTCTGC AAGAGTTGGA AAACTGTGAA
    AATGATCCTG TGGCCATAGC AGAGTGCTTT GTGTCCAAGA GTGAAGAGTT CCACATTTAT
    ACCCAGTATT GCACGAACTA TCCAAGATCT GTGGCTGTGC TAACAGAGTG TATGAGGAAC
    AAGATGCTGG CCAAATTCTT CAGGGAACGG CAGGAAACTT TGAAACATTC ATTGCCTCTG
    GGGTCCTATC TCTTGAAGCC AGTTCAGCGG ATTCTCAAGT ATCATCTCCT TTTGCATGAA
    ATAGAAAATC ACCTTGACAA GGACACAGAG GGCTACGATG TGGTGCTTGA TGCCATAGAC
    ACGATGCAGA GAGTGGCCTG GCACATCAAT GACATGAAGC GGAAGCATGA ACACGCCGTC
    CGCCTGCAGG AGATACAGAG TTTGCTCACC AACTGGAAGG GGCCAGACCT GACCAGCTAC
    GGGGAGCTAG TGCTCGAAGG GACCTTCCGC CTCCAGCGGG CCAAGAATGA GCGGACGCTC
    TTCCTCTTTG ACAAGCTGCT GCTCATCACC AAGAAGAGAG ACGACACGTT TACATACAAA
    GCCCACATCC TGTGCGGCAA CCTCATGCTG GTGGAAGTGA TCCCAAAGGA GCCCCTCAGC
    TTCAGCGTCT TCCACTACAA GAACCCCAAG CTGCAGCACA CGGTTCAGGC GAAGTCGCAG
    CAAGACAAGC GCCTGTGGGT TCTGCACCTG AAGAGGCTGA TCCTGGAGAA TCATGCGGCA
    AAGATTCCAG CTAAGGCCAA GCAAGCAATA CTTGAAATGG ACGCCATTCA TTACCCAGGC
    TTCTGTTACA GTCCCGAGGG AGAGACTTGT GGCTCTCGAA ATGGCTCTGC TCCGTATCGG
    CTGAGAAGGA AATCTGAACC TTCCTCAAGA TCACATAAAG TTTTGAAGAC CAGCGAAACA
    GCACAAGACA TTCAAAAGGT TTCCAGAGAG GAAGGGTCTC CCCAGTTGAC TTCAGCAGGG
    CCCTCTCCTG CCCAGCGGAA CAGTCAGCCA AGCAGTTCGA CCATCCTGAG CGTGCTTCGT
    GGGGGTGGGG CTGTCAGAAA CATCTGGACC GACCACCAGA TCCGCCAAGC CCTGTTTCCC
    AGCCGAAGGT CGCAGGAGAA CGAGGAGGAT GAAGACGACT ATCAGATGTT CCTGCCATCC
    TTCTCCTCCT CGGATCTGAA CTCAGCCCAG CTGTGTGAAG ATGGTACTTC TAGTCGACCT
    TGCAGTTGGC ACATGGGACA AATCGAGTCC TCCGAGACCT CCAGCTCTGG CCACAGGATT
    GTGCGGCGGG CCAGCAGTGC TGGCGAGAGC AACACGTGTC CCCCTGACAT AAGAACTAGG
    GACAGTGGTG GTTCTCAGTA CAGCTCCCGG AGGGAACTGC AGGGTGACCC TAAAACAGAG
    GGGCAGGACG GGACAACTCC CTACGGGTCC TCTATAGAGT TGACTATAGA TGACATAGAC
    CATGTCTATG ACAACATAAG TTACGAGGAC TTGAAACTGA TGGTTGCTAA ACGCGAAGAC
    GCTGAAGTCA GTCCCCAGAA ACCAGTCAGG GACTCTGTTC GCCCCAAGAG CACCCCAGAG
    CTGGCCTTCT CCAAGAAGCC AGCGGGGCCT GAGGAGGAGA GCACATTGCA CCCCAGGAGA
    GATGGAGCCC TCCCGGCCGG GGAGGCCTGG AACCAAAGCA TGCACGACCT CCAGGTGATG
    GAGGAGAATG TCTATGACAC CATTGCGCTC CCGGAGGCGC CCTCCCGGGA CTTCAAGGGC
    AGCAGCCCGA AGCGTTCTGC AAGGAGCACC TTCCTGGGCT TGGAGGCCGA CTTGGGGTGC
    TGTGATAGTC TGCGGGCCTC TGCTTCCCAG GACAGCCTCC AGTTCAGTGA GGACGAGGCG
    CCCTACCCGC CAGGCTCCTC TGATAACGAT TATCTGAGTT TGCTCTATAA CTCCTTCGGC
    TGCAACTTGG CCCTAGCGGA TCGGTCCATC TCTGATAAAC TGTCTGAAGA AGTAGATGAA
    ATCTGGAATG ACTTGGAAAA CTACATCAAG AAAAACGAAG TCAAAGCCAG AAACCGGCTC
    CTGGCCGCGT TTCCGGTTAG CAAAGATGAC GTGCAGGAGC GGCCGCTGGC GGGCAGTACC
    CCGGAGCTGA GCGCGCGCTC CCCGCTCTCG CTGGCCGCGG GCCGGGCTCT CCTCGCGCCG
    CCGGCAGACA GGCCCGGGCC AGCCCCCTGC GCCCTGGACC GAGACCCAGC CTCCAAAGAC
    AGCTCCTGCC TGAGTCTCCA CCGGCTGTCG CTGGCCAGTG ACCTGCCGCC CGCAGAGAGT
    CCCTATGATG TGGCCGGCGG CAGCCTGTCC TCAGCGGACC TAGAGAACCC TGAGCCCGGG
    CTGGACAATG TAGACAAGAC CAGGAGCAGG GTTTTCCTGA TGGCCAGGCA GTACAGTCAG
    AAGATCAAGA AGGCAAATCA GCTGCTAAAA GTGAAAAACC CAGAGCTGGA ACAGACGCTC
    AGTAGCCAGC CGCAGAAGCC CGCGTCCAAG GACCTGGCGG CCATCCTAGA AGAGAAGAGG
    CAGGGCGGGC CCGCCATCGG TGCCAGGATT GCTGAGTATT CCCAACTGTA TGACCAGATT
    GTATTTAGAG AGACCCCCTT TAAGACTCAG AAGGATGGCT GGGCCAGCCC TCAAGACCCC
    TCCAACCTCA GCTCTGCATC ACCTTCCCAT GCCCAGCAAG GTAGCGAGGA CTGGCTTTTG
    CATTCAACCT ATAGTAATGG AGAGTTAGCT GACTTCTGTC CCTGGCCAGA ACAAGACTTA
    AAGTCAAAAT ATCCCATCTT AGAGATCAAT GCAAGAAGTA CCCCAAAACA ACTGTCTGCA
    GCTTGCTCTG TGCCTTCTCT TCAAACCTCC GACCCTCTGC TGGGCTTCGT GCAGAGACAC
    AGCGTGGTGG TCAGCCAGCC AAACAAAGAG AACTCCTGCC AGGGCCATCT TTACAACTCT
    CTGGGTCGGA AAGGAATCAG TGCTAAATCT CAGCCTTATA ACAGGTCCCA ATCATCTTCC
    TCCATCCTGA TAAACAAGTC TGCAGACTCC ATCAACTACC CTAGTGGCGT GGCAAAGCAG
    CAGCCTGTCT CTCTACACAG AAGTTCAAGG TGGGAGAGTC ACCAGGACTT GCTGCCAAGT
    ATTGCTGACT CAGGTCAACA GGGCCCTGAA AAACGCTCAG ATCTCACACT CCAGGACTCG
    CAGAAAGTTC TGGTAGTGAA TAGAAATTTA CCTTTAAGTG CTCAAATGGC TACCCAGAAC
    TATTTTTCCA ATTTCAAAGA GACTGAAGGA GACGAAGATG ACTATGTGGA GATCAAGTCA
    GAAGAAGATG AGTCTGAGTT AGAACTGTCT CACAGTCGGA GGAGGAAATC TGACCCAAAG
    ATTACAGACG CTGACTTTGC AGATAACGTC TGCAGCCACA CGACTTCTTA CTCTTTGAAT
    AGTCCGTGCA CTCCAAAAAA GCCAGTAAGT GGCAAACTTG GGATTTCGCC GTATCTGACG
    CCATACAATG ATCCCGACAA ACTGAATGAC TATCTCTGGA AGGGCCCATC TCCCAATCAG
    CAAAATATTG TGCAGTCTCT AAGGGAAAAA TTCCAGTGTC TTAGTTCAAG CAGCTTTGCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192850.1
    CDS211..4353
    Translation

    Target ORF information:

    RefSeq Version NM_001205921.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology domain containing, family G (with RhoGef domain) member 1 (PLEKHG1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001205921.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAGCTCT CCGACAGTGA CCGCCCGGTC AGCTTCGGCT CCACGTCGTC CTCGGCTTCC 
    TCCCGGGACA GCCATGGTTC CTTCGGCAGC AGAATGACCT TAGTTTCAAG CAGCCATCTG
    GGCTTGTTTC CTCAGGATAA GGAAGCAGGG GCCATAAAAC TGGAGCTGAT GCCAGCCAGG
    CCGTTTTCCA GCAGCGAGCT GCAGACGGAC CGTGCGGCCG GGCAGCAGAG CACAGACGAG
    GGCGGCGAGA GGCAGCCCCG GGCACAGCGC AGAGTGGAAG CCAACGGGCC GACCAAAACC
    GGGGCCGAAT CGGCCACCAG CCCCAAGCTG CTCTATGTGG ACAGAGTTGT GCAGGAAATT
    CTGGAGACCG AACGGACTTA CGTGCAAGAT TTAAAGAGCA TCGTAGAGGA TTACCTTGAC
    TGTATCAGGG ACCAAACGAA ACTTCCTCTG GGGACAGATG AAAGATCAGC CCTTTTTGGA
    AACATACAGG ATATCTACCA CTTTAATAGT GAACTTCTGC AAGAGTTGGA AAACTGTGAA
    AATGATCCTG TGGCCATAGC AGAGTGCTTT GTGTCCAAGA GTGAAGAGTT CCACATTTAT
    ACCCAGTATT GCACGAACTA TCCAAGATCT GTGGCTGTGC TAACAGAGTG TATGAGGAAC
    AAGATGCTGG CCAAATTCTT CAGGGAACGG CAGGAAACTT TGAAACATTC ATTGCCTCTG
    GGGTCCTATC TCTTGAAGCC AGTTCAGCGG ATTCTCAAGT ATCATCTCCT TTTGCATGAA
    ATAGAAAATC ACCTTGACAA GGACACAGAG GGCTACGATG TGGTGCTTGA TGCCATAGAC
    ACGATGCAGA GAGTGGCCTG GCACATCAAT GACATGAAGC GGAAGCATGA ACACGCCGTC
    CGCCTGCAGG AGATACAGAG TTTGCTCACC AACTGGAAGG GGCCAGACCT GACCAGCTAC
    GGGGAGCTAG TGCTCGAAGG GACCTTCCGC CTCCAGCGGG CCAAGAATGA GCGGACGCTC
    TTCCTCTTTG ACAAGCTGCT GCTCATCACC AAGAAGAGAG ACGACACGTT TACATACAAA
    GCCCACATCC TGTGCGGCAA CCTCATGCTG GTGGAAGTGA TCCCAAAGGA GCCCCTCAGC
    TTCAGCGTCT TCCACTACAA GAACCCCAAG CTGCAGCACA CGGTTCAGGC GAAGTCGCAG
    CAAGACAAGC GCCTGTGGGT TCTGCACCTG AAGAGGCTGA TCCTGGAGAA TCATGCGGCA
    AAGATTCCAG CTAAGGCCAA GCAAGCAATA CTTGAAATGG ACGCCATTCA TTACCCAGGC
    TTCTGTTACA GTCCCGAGGG AGAGACTTGT GGCTCTCGAA ATGGCTCTGC TCCGTATCGG
    CTGAGAAGGA AATCTGAACC TTCCTCAAGA TCACATAAAG TTTTGAAGAC CAGCGAAACA
    GCACAAGACA TTCAAAAGGT TTCCAGAGAG GAAGGGTCTC CCCAGTTGAC TTCAGCAGGG
    CCCTCTCCTG CCCAGCGGAA CAGTCAGCCA AGCAGTTCGA CCATCCTGAG CGTGCTTCGT
    GGGGGTGGGG CTGTCAGAAA CATCTGGACC GACCACCAGA TCCGCCAAGC CCTGTTTCCC
    AGCCGAAGGT CGCAGGAGAA CGAGGAGGAT GAAGACGACT ATCAGATGTT CCTGCCATCC
    TTCTCCTCCT CGGATCTGAA CTCAGCCCAG CTGTGTGAAG ATGGTACTTC TAGTCGACCT
    TGCAGTTGGC ACATGGGACA AATCGAGTCC TCCGAGACCT CCAGCTCTGG CCACAGGATT
    GTGCGGCGGG CCAGCAGTGC TGGCGAGAGC AACACGTGTC CCCCTGACAT AAGAACTAGG
    GACAGTGGTG GTTCTCAGTA CAGCTCCCGG AGGGAACTGC AGGGTGACCC TAAAACAGAG
    GGGCAGGACG GGACAACTCC CTACGGGTCC TCTATAGAGT TGACTATAGA TGACATAGAC
    CATGTCTATG ACAACATAAG TTACGAGGAC TTGAAACTGA TGGTTGCTAA ACGCGAAGAC
    GCTGAAGTCA GTCCCCAGAA ACCAGTCAGG GACTCTGTTC GCCCCAAGAG CACCCCAGAG
    CTGGCCTTCT CCAAGAAGCC AGCGGGGCCT GAGGAGGAGA GCACATTGCA CCCCAGGAGA
    GATGGAGCCC TCCCGGCCGG GGAGGCCTGG AACCAAAGCA TGCACGACCT CCAGGTGATG
    GAGGAGAATG TCTATGACAC CATTGCGCTC CCGGAGGCGC CCTCCCGGGA CTTCAAGGGC
    AGCAGCCCGA AGCGTTCTGC AAGGAGCACC TTCCTGGGCT TGGAGGCCGA CTTGGGGTGC
    TGTGATAGTC TGCGGGCCTC TGCTTCCCAG GACAGCCTCC AGTTCAGTGA GGACGAGGCG
    CCCTACCCGC CAGGCTCCTC TGATAACGAT TATCTGAGTT TGCTCTATAA CTCCTTCGGC
    TGCAACTTGG CCCTAGCGGA TCGGTCCATC TCTGATAAAC TGTCTGAAGA AGTAGATGAA
    ATCTGGAATG ACTTGGAAAA CTACATCAAG AAAAACGAAG TCAAAGCCAG AAACCGGCTC
    CTGGCCGCGT TTCCGGTTAG CAAAGATGAC GTGCAGGAGC GGCCGCTGGC GGGCAGTACC
    CCGGAGCTGA GCGCGCGCTC CCCGCTCTCG CTGGCCGCGG GCCGGGCTCT CCTCGCGCCG
    CCGGCAGACA GGCCCGGGCC AGCCCCCTGC GCCCTGGACC GAGACCCAGC CTCCAAAGAC
    AGCTCCTGCC TGAGTCTCCA CCGGCTGTCG CTGGCCAGTG ACCTGCCGCC CGCAGAGAGT
    CCCTATGATG TGGCCGGCGG CAGCCTGTCC TCAGCGGACC TAGAGAACCC TGAGCCCGGG
    CTGGACAATG TAGACAAGAC CAGGAGCAGG GTTTTCCTGA TGGCCAGGCA GTACAGTCAG
    AAGATCAAGA AGGCAAATCA GCTGCTAAAA GTGAAAAACC CAGAGCTGGA ACAGACGCTC
    AGTAGCCAGC CGCAGAAGCC CGCGTCCAAG GACCTGGCGG CCATCCTAGA AGAGAAGAGG
    CAGGGCGGGC CCGCCATCGG TGCCAGGATT GCTGAGTATT CCCAACTGTA TGACCAGATT
    GTATTTAGAG AGACCCCCTT TAAGACTCAG AAGGATGGCT GGGCCAGCCC TCAAGACCCC
    TCCAACCTCA GCTCTGCATC ACCTTCCCAT GCCCAGCAAG GTAGCGAGGA CTGGCTTTTG
    CATTCAACCT ATAGTAATGG AGAGTTAGCT GACTTCTGTC CCTGGCCAGA ACAAGACTTA
    AAGTCAAAAT ATCCCATCTT AGAGATCAAT GCAAGAAGTA CCCCAAAACA ACTGTCTGCA
    GCTTGCTCTG TGCCTTCTCT TCAAACCTCC GACCCTCTGC TGGGCTTCGT GCAGAGACAC
    AGCGTGGTGG TCAGCCAGCC AAACAAAGAG AACTCCTGCC AGGGCCATCT TTACAACTCT
    CTGGGTCGGA AAGGAATCAG TGCTAAATCT CAGCCTTATA ACAGGTCCCA ATCATCTTCC
    TCCATCCTGA TAAACAAGTC TGCAGACTCC ATCAACTACC CTAGTGGCGT GGCAAAGCAG
    CAGCCTGTCT CTCTACACAG AAGTTCAAGG TGGGAGAGTC ACCAGGACTT GCTGCCAAGT
    ATTGCTGACT CAGGTCAACA GGGCCCTGAA AAACGCTCAG ATCTCACACT CCAGGACTCG
    CAGAAAGTTC TGGTAGTGAA TAGAAATTTA CCTTTAAGTG CTCAAATGGC TACCCAGAAC
    TATTTTTCCA ATTTCAAAGA GACTGAAGGA GACGAAGATG ACTATGTGGA GATCAAGTCA
    GAAGAAGATG AGTCTGAGTT AGAACTGTCT CACAGTCGGA GGAGGAAATC TGACCCAAAG
    ATTACAGACG CTGACTTTGC AGATAACGTC TGCAGCCACA CGACTTCTTA CTCTTTGAAT
    AGTCCGTGCA CTCCAAAAAA GCCAGTAAGT GGCAAACTTG GGATTTCGCC GTATCTGACG
    CCATACAATG ATCCCGACAA ACTGAATGAC TATCTCTGGA AGGGCCCATC TCCCAATCAG
    CAAAATATTG TGCAGTCTCT AAGGGAAAAA TTCCAGTGTC TTAGTTCAAG CAGCTTTGCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237778
    Clone ID Related Accession (Same CDS sequence) XM_024996655.1 , XM_024996658.1 , XM_024996654.1 , NM_001205921.2 , XM_024996656.1
    Accession Version NM_001205921.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4143bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-14
    Organism Bos taurus(cattle)
    Product pleckstrin homology domain-containing family G member 1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000009.1. On Jun 15, 2018 this sequence version replaced NM_001205921.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## CDS exon combination :: SRR5571265.33738.1, SRR5571260.46991.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN03145425, SAMN03145426 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAGCTCT CCGACAGTGA CCGCCCGGTC AGCTTCGGCT CCACGTCGTC CTCGGCTTCC 
    TCCCGGGACA GCCATGGTTC CTTCGGCAGC AGAATGACCT TAGTTTCAAG CAGCCATCTG
    GGCTTGTTTC CTCAGGATAA GGAAGCAGGG GCCATAAAAC TGGAGCTGAT GCCAGCCAGG
    CCGTTTTCCA GCAGCGAGCT GCAGACGGAC CGTGCGGCCG GGCAGCAGAG CACAGACGAG
    GGCGGCGAGA GGCAGCCCCG GGCACAGCGC AGAGTGGAAG CCAACGGGCC GACCAAAACC
    GGGGCCGAAT CGGCCACCAG CCCCAAGCTG CTCTATGTGG ACAGAGTTGT GCAGGAAATT
    CTGGAGACCG AACGGACTTA CGTGCAAGAT TTAAAGAGCA TCGTAGAGGA TTACCTTGAC
    TGTATCAGGG ACCAAACGAA ACTTCCTCTG GGGACAGATG AAAGATCAGC CCTTTTTGGA
    AACATACAGG ATATCTACCA CTTTAATAGT GAACTTCTGC AAGAGTTGGA AAACTGTGAA
    AATGATCCTG TGGCCATAGC AGAGTGCTTT GTGTCCAAGA GTGAAGAGTT CCACATTTAT
    ACCCAGTATT GCACGAACTA TCCAAGATCT GTGGCTGTGC TAACAGAGTG TATGAGGAAC
    AAGATGCTGG CCAAATTCTT CAGGGAACGG CAGGAAACTT TGAAACATTC ATTGCCTCTG
    GGGTCCTATC TCTTGAAGCC AGTTCAGCGG ATTCTCAAGT ATCATCTCCT TTTGCATGAA
    ATAGAAAATC ACCTTGACAA GGACACAGAG GGCTACGATG TGGTGCTTGA TGCCATAGAC
    ACGATGCAGA GAGTGGCCTG GCACATCAAT GACATGAAGC GGAAGCATGA ACACGCCGTC
    CGCCTGCAGG AGATACAGAG TTTGCTCACC AACTGGAAGG GGCCAGACCT GACCAGCTAC
    GGGGAGCTAG TGCTCGAAGG GACCTTCCGC CTCCAGCGGG CCAAGAATGA GCGGACGCTC
    TTCCTCTTTG ACAAGCTGCT GCTCATCACC AAGAAGAGAG ACGACACGTT TACATACAAA
    GCCCACATCC TGTGCGGCAA CCTCATGCTG GTGGAAGTGA TCCCAAAGGA GCCCCTCAGC
    TTCAGCGTCT TCCACTACAA GAACCCCAAG CTGCAGCACA CGGTTCAGGC GAAGTCGCAG
    CAAGACAAGC GCCTGTGGGT TCTGCACCTG AAGAGGCTGA TCCTGGAGAA TCATGCGGCA
    AAGATTCCAG CTAAGGCCAA GCAAGCAATA CTTGAAATGG ACGCCATTCA TTACCCAGGC
    TTCTGTTACA GTCCCGAGGG AGAGACTTGT GGCTCTCGAA ATGGCTCTGC TCCGTATCGG
    CTGAGAAGGA AATCTGAACC TTCCTCAAGA TCACATAAAG TTTTGAAGAC CAGTGAAACA
    GCACAAGACA TTCAAAAGGT TTCCAGAGAG GAAGGGTCTC CCCAGTTGAC TTCAGCAGGG
    CCCTCTCCTG CCCAGCGGAA CAGTCAGCCA AGCAGTTCGA CCATCCTGAG CGTGCTTCGT
    GGGGGTGGGG CTGTCAGAAA CATCTGGACC GACCACCAGA TCCGCCAAGC CCTGTTTCCC
    AGCCGAAGGT CGCAGGAGAA CGAGGAGGAT GAAGACGACT ATCAGATGTT CCTGCCATCC
    TTCTCCTCCT CGGATCTGAA CTCAGCCCAG CTGTGTGAAG ATGGTACTTC TAGTCGACCT
    TGCAGTTGGC ACATGGGACA AATCGAGTCC TCCGAGACCT CCAGCTCTGG CCACAGGATT
    GTGCGGCGGG CCAGCAGTGC TGGCGAGAGC AACACGTGTC CCCCTGACAT AAGAACTAGG
    GACAGTGGTG GTTCTCAGTA CAGCTCCCGG AGGGAACTGC AGGGTGACCC TAAAACAGAG
    GGGCAGGACG GGACAACTCC CTACGGGTCC TCTATAGAGT TGACTATAGA TGACATAGAC
    CATGTCTATG ACAACATAAG TTACGAGGAC TTGAAACTGA TGGTTGCTAA ACGCGAAGAC
    GCTGAAGTCA GTCCCCAGAA ACCAGTCAGG GACTCTGTTC GCCCCAAGAG CACCCCAGAG
    CTGGCCTTCT CCAAGAAGCC AGCGGGGCCT GAGGAGGAGA GCACATTGCA CCCCAGGAGA
    GATGGAGCCC TCCCGGCCGG GGAGGCCTGG AACCAAAGCA TGCACGACCT CCAGGTGATG
    GAGGAGAATG TCTATGACAC CATTGCGCTC CCGGAGGCGC CCTCCCGGGA CTTCAAGGGC
    AGCAGCCCGA AGCGTTCTGC AAGGAGCACC TTCCTGGGCT TGGAGGCCGA CTTGGGGTGC
    TGTGATAGTC TGCGGGCCTC TGCTTCCCAG GACAGCCTCC AGTTCAGTGA GGACGAGGCG
    CCCTACCCGC CAGGCTCCTC TGATAACGAT TATCTGAGTT TGCTCTATAA CTCCTTCGGC
    TGCAACTTGG CCCTAGCGGA TCGGTCCATC TCTGATAAAC TGTCTGAAGA AGTAGATGAA
    ATCTGGAATG ACTTGGAAAA CTACATCAAG AAAAACGAAG TCAAAGCCAG AAACCGGCTC
    CTGGCCGCGT TTCCGGTTAG CAAAGATGAC GTGCAGGAGC GGCCGCTGGC GGGCAGTACC
    CCGGAGCTGA GCGCGCGCTC CCCGCTCTCG CTGGCCGCGG GCCGGGCTCT CCTCGCGCCG
    CCGGCAGACA GGCCCGGGCC AGCCCCCTGC GCCCTGGACC GAGACCCAGC CTCCAAAGAC
    AGCTCCTGCC TGAGTCTCCA CCGGCTGTCG CTGGCCAGTG ACCTGCCGCC CGCAGAGAGT
    CCCTATGATG TGGCCGGCGG CAGCCTGTCC TCAGCGGACC TAGAGAACCC TGAGCCCGGG
    CTGGACAATG TAGACAAGAC CAGGAGCAGG GTTTTCCTGA TGGCCAGGCA GTACAGTCAG
    AAGATCAAGA AGGCAAATCA GCTGCTAAAA GTGAAAAACC CAGAGCTGGA ACAGACGCTC
    AGTAGCCAGC CGCAGAAGCC CGCGTCCAAG GACCTGGCGG CCATCCTAGA AGAGAAGAGG
    CAGGGCGGGC CCGCCATCGG TGCCAGGATT GCTGAGTATT CCCAACTGTA TGACCAGATT
    GTATTTAGAG AGACCCCCTT TAAGACTCAG AAGGATGGCT GGGCCAGCCC TCAAGACCCC
    TCCAACCTCA GCTCTGCATC ACCTTCCCAT GCCCAGCAAG GTAGCGAGGA CTGGCTTTTG
    CATTCAACCT ATAGTAATGG AGAGTTAGCT GACTTCTGTC CCTGGCCAGA ACAAGACTTA
    AAGTCAAAAT ATCCCATCTT AGAGATCAAT GCAAGAAGTA CCCCAAAACA ACTGTCTGCA
    GCTTGCTCTG TGCCTTCTCT TCAAACCTCC GACCCTCTGC TGGGCTTCGT GCAGAGACAC
    AGCGTGGTGG TCAGCCAGCC AAACAAAGAG AACTCCTGCC AGGGCCATCT TTACAACTCT
    CTGGGTCGGA AAGGAATCAG TGCTAAATCT CAGCCTTATA ACAGGTCCCA ATCATCTTCC
    TCCATCCTGA TAAACAAGTC TGCAGACTCC ATCAACTACC CTAGTGGCGT GGCAAAGCAG
    CAGCCTGTCT CTCTACACAG AAGTTCAAGG TGGGAGAGTC ACCAGGACTT GCTGCCAAGT
    ATTGCTGACT CAGGTCAACA GGGCCCTGAA AAACGCTCAG ATCTCACACT CCAGGACTCG
    CAGAAAGTTC TGGTAGTGAA TAGAAATTTA CCTTTAAGTG CTCAAATGGC TACCCAGAAC
    TATTTTTCCA ATTTCAAAGA GACTGAAGGA GACGAAGATG ACTATGTGGA GATCAAGTCA
    GAAGAAGATG AGTCTGAGTT AGAACTGTCT CACAGTCGGA GGAGGAAATC TGACCCAAAG
    ATTACAGACG CTGACTTTGC AGATAACGTC TGCAGCCACA CGACTTCTTA CTCTTTGAAT
    AGTCCGTGCA CTCCAAAAAA GCCAGTAAGT GGCAAACTTG GGATTTCGCC GTATCTGACG
    CCATACAATG ATCCCGACAA ACTGAATGAC TATCTCTGGA AGGGCCCATC TCCCAATCAG
    CAAAATATTG TGCAGTCTCT AAGGGAAAAA TTCCAGTGTC TTAGTTCAAG CAGCTTTGCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192850.1
    CDS212..4354
    Translation

    Target ORF information:

    RefSeq Version NM_001205921.2
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology and RhoGEF domain containing G1 (PLEKHG1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001205921.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAGCTCT CCGACAGTGA CCGCCCGGTC AGCTTCGGCT CCACGTCGTC CTCGGCTTCC 
    TCCCGGGACA GCCATGGTTC CTTCGGCAGC AGAATGACCT TAGTTTCAAG CAGCCATCTG
    GGCTTGTTTC CTCAGGATAA GGAAGCAGGG GCCATAAAAC TGGAGCTGAT GCCAGCCAGG
    CCGTTTTCCA GCAGCGAGCT GCAGACGGAC CGTGCGGCCG GGCAGCAGAG CACAGACGAG
    GGCGGCGAGA GGCAGCCCCG GGCACAGCGC AGAGTGGAAG CCAACGGGCC GACCAAAACC
    GGGGCCGAAT CGGCCACCAG CCCCAAGCTG CTCTATGTGG ACAGAGTTGT GCAGGAAATT
    CTGGAGACCG AACGGACTTA CGTGCAAGAT TTAAAGAGCA TCGTAGAGGA TTACCTTGAC
    TGTATCAGGG ACCAAACGAA ACTTCCTCTG GGGACAGATG AAAGATCAGC CCTTTTTGGA
    AACATACAGG ATATCTACCA CTTTAATAGT GAACTTCTGC AAGAGTTGGA AAACTGTGAA
    AATGATCCTG TGGCCATAGC AGAGTGCTTT GTGTCCAAGA GTGAAGAGTT CCACATTTAT
    ACCCAGTATT GCACGAACTA TCCAAGATCT GTGGCTGTGC TAACAGAGTG TATGAGGAAC
    AAGATGCTGG CCAAATTCTT CAGGGAACGG CAGGAAACTT TGAAACATTC ATTGCCTCTG
    GGGTCCTATC TCTTGAAGCC AGTTCAGCGG ATTCTCAAGT ATCATCTCCT TTTGCATGAA
    ATAGAAAATC ACCTTGACAA GGACACAGAG GGCTACGATG TGGTGCTTGA TGCCATAGAC
    ACGATGCAGA GAGTGGCCTG GCACATCAAT GACATGAAGC GGAAGCATGA ACACGCCGTC
    CGCCTGCAGG AGATACAGAG TTTGCTCACC AACTGGAAGG GGCCAGACCT GACCAGCTAC
    GGGGAGCTAG TGCTCGAAGG GACCTTCCGC CTCCAGCGGG CCAAGAATGA GCGGACGCTC
    TTCCTCTTTG ACAAGCTGCT GCTCATCACC AAGAAGAGAG ACGACACGTT TACATACAAA
    GCCCACATCC TGTGCGGCAA CCTCATGCTG GTGGAAGTGA TCCCAAAGGA GCCCCTCAGC
    TTCAGCGTCT TCCACTACAA GAACCCCAAG CTGCAGCACA CGGTTCAGGC GAAGTCGCAG
    CAAGACAAGC GCCTGTGGGT TCTGCACCTG AAGAGGCTGA TCCTGGAGAA TCATGCGGCA
    AAGATTCCAG CTAAGGCCAA GCAAGCAATA CTTGAAATGG ACGCCATTCA TTACCCAGGC
    TTCTGTTACA GTCCCGAGGG AGAGACTTGT GGCTCTCGAA ATGGCTCTGC TCCGTATCGG
    CTGAGAAGGA AATCTGAACC TTCCTCAAGA TCACATAAAG TTTTGAAGAC CAGTGAAACA
    GCACAAGACA TTCAAAAGGT TTCCAGAGAG GAAGGGTCTC CCCAGTTGAC TTCAGCAGGG
    CCCTCTCCTG CCCAGCGGAA CAGTCAGCCA AGCAGTTCGA CCATCCTGAG CGTGCTTCGT
    GGGGGTGGGG CTGTCAGAAA CATCTGGACC GACCACCAGA TCCGCCAAGC CCTGTTTCCC
    AGCCGAAGGT CGCAGGAGAA CGAGGAGGAT GAAGACGACT ATCAGATGTT CCTGCCATCC
    TTCTCCTCCT CGGATCTGAA CTCAGCCCAG CTGTGTGAAG ATGGTACTTC TAGTCGACCT
    TGCAGTTGGC ACATGGGACA AATCGAGTCC TCCGAGACCT CCAGCTCTGG CCACAGGATT
    GTGCGGCGGG CCAGCAGTGC TGGCGAGAGC AACACGTGTC CCCCTGACAT AAGAACTAGG
    GACAGTGGTG GTTCTCAGTA CAGCTCCCGG AGGGAACTGC AGGGTGACCC TAAAACAGAG
    GGGCAGGACG GGACAACTCC CTACGGGTCC TCTATAGAGT TGACTATAGA TGACATAGAC
    CATGTCTATG ACAACATAAG TTACGAGGAC TTGAAACTGA TGGTTGCTAA ACGCGAAGAC
    GCTGAAGTCA GTCCCCAGAA ACCAGTCAGG GACTCTGTTC GCCCCAAGAG CACCCCAGAG
    CTGGCCTTCT CCAAGAAGCC AGCGGGGCCT GAGGAGGAGA GCACATTGCA CCCCAGGAGA
    GATGGAGCCC TCCCGGCCGG GGAGGCCTGG AACCAAAGCA TGCACGACCT CCAGGTGATG
    GAGGAGAATG TCTATGACAC CATTGCGCTC CCGGAGGCGC CCTCCCGGGA CTTCAAGGGC
    AGCAGCCCGA AGCGTTCTGC AAGGAGCACC TTCCTGGGCT TGGAGGCCGA CTTGGGGTGC
    TGTGATAGTC TGCGGGCCTC TGCTTCCCAG GACAGCCTCC AGTTCAGTGA GGACGAGGCG
    CCCTACCCGC CAGGCTCCTC TGATAACGAT TATCTGAGTT TGCTCTATAA CTCCTTCGGC
    TGCAACTTGG CCCTAGCGGA TCGGTCCATC TCTGATAAAC TGTCTGAAGA AGTAGATGAA
    ATCTGGAATG ACTTGGAAAA CTACATCAAG AAAAACGAAG TCAAAGCCAG AAACCGGCTC
    CTGGCCGCGT TTCCGGTTAG CAAAGATGAC GTGCAGGAGC GGCCGCTGGC GGGCAGTACC
    CCGGAGCTGA GCGCGCGCTC CCCGCTCTCG CTGGCCGCGG GCCGGGCTCT CCTCGCGCCG
    CCGGCAGACA GGCCCGGGCC AGCCCCCTGC GCCCTGGACC GAGACCCAGC CTCCAAAGAC
    AGCTCCTGCC TGAGTCTCCA CCGGCTGTCG CTGGCCAGTG ACCTGCCGCC CGCAGAGAGT
    CCCTATGATG TGGCCGGCGG CAGCCTGTCC TCAGCGGACC TAGAGAACCC TGAGCCCGGG
    CTGGACAATG TAGACAAGAC CAGGAGCAGG GTTTTCCTGA TGGCCAGGCA GTACAGTCAG
    AAGATCAAGA AGGCAAATCA GCTGCTAAAA GTGAAAAACC CAGAGCTGGA ACAGACGCTC
    AGTAGCCAGC CGCAGAAGCC CGCGTCCAAG GACCTGGCGG CCATCCTAGA AGAGAAGAGG
    CAGGGCGGGC CCGCCATCGG TGCCAGGATT GCTGAGTATT CCCAACTGTA TGACCAGATT
    GTATTTAGAG AGACCCCCTT TAAGACTCAG AAGGATGGCT GGGCCAGCCC TCAAGACCCC
    TCCAACCTCA GCTCTGCATC ACCTTCCCAT GCCCAGCAAG GTAGCGAGGA CTGGCTTTTG
    CATTCAACCT ATAGTAATGG AGAGTTAGCT GACTTCTGTC CCTGGCCAGA ACAAGACTTA
    AAGTCAAAAT ATCCCATCTT AGAGATCAAT GCAAGAAGTA CCCCAAAACA ACTGTCTGCA
    GCTTGCTCTG TGCCTTCTCT TCAAACCTCC GACCCTCTGC TGGGCTTCGT GCAGAGACAC
    AGCGTGGTGG TCAGCCAGCC AAACAAAGAG AACTCCTGCC AGGGCCATCT TTACAACTCT
    CTGGGTCGGA AAGGAATCAG TGCTAAATCT CAGCCTTATA ACAGGTCCCA ATCATCTTCC
    TCCATCCTGA TAAACAAGTC TGCAGACTCC ATCAACTACC CTAGTGGCGT GGCAAAGCAG
    CAGCCTGTCT CTCTACACAG AAGTTCAAGG TGGGAGAGTC ACCAGGACTT GCTGCCAAGT
    ATTGCTGACT CAGGTCAACA GGGCCCTGAA AAACGCTCAG ATCTCACACT CCAGGACTCG
    CAGAAAGTTC TGGTAGTGAA TAGAAATTTA CCTTTAAGTG CTCAAATGGC TACCCAGAAC
    TATTTTTCCA ATTTCAAAGA GACTGAAGGA GACGAAGATG ACTATGTGGA GATCAAGTCA
    GAAGAAGATG AGTCTGAGTT AGAACTGTCT CACAGTCGGA GGAGGAAATC TGACCCAAAG
    ATTACAGACG CTGACTTTGC AGATAACGTC TGCAGCCACA CGACTTCTTA CTCTTTGAAT
    AGTCCGTGCA CTCCAAAAAA GCCAGTAAGT GGCAAACTTG GGATTTCGCC GTATCTGACG
    CCATACAATG ATCCCGACAA ACTGAATGAC TATCTCTGGA AGGGCCCATC TCCCAATCAG
    CAAAATATTG TGCAGTCTCT AAGGGAAAAA TTCCAGTGTC TTAGTTCAAG CAGCTTTGCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237775
    Clone ID Related Accession (Same CDS sequence) XM_024996651.1
    Accession Version XM_024996651.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4434bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pleckstrin homology domain-containing family G member 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCTGTCCA CAACCATGGT GGGGGCTCTG GTCAGAGGGT GGAGAAATCG AAGCTCTCCT 
    CGAAGGGCAG AATCCAAGCT TATTATCCCT CAGAGCCAAG ATGAAATTCA GACTCGAGTT
    CTGCTGGGAG CAGAGTCAGA TGATAAAGTC AGCTCACCAC AAGATATTGA GGACCTGCCG
    GGGGAAGTTT CAGATGCGCA CCAGTTGCGT CGTGAAGTAC GGTCAGCTGT AACACAAGCT
    CGGTATCTCA GAACCACAGC CAGGCACTCC TGCTCCGGGG ACCTCGGGGC CATGGAGCTC
    TCCGACAGTG ACCGCCCGGT CAGCTTCGGC TCCACGTCGT CCTCGGCTTC CTCCCGGGAC
    AGCCATGGTT CCTTCGGCAG CAGAATGACC TTAGTTTCAA GCAGCCATCT GGGCTTGTTT
    CCTCAGGATA AGGAAGCAGG GGCCATAAAA CTGGAGCTGA TGCCAGCCAG GCCGTTTTCC
    AGCAGCGAGC TGCAGACGGA CCGTGCGGCC GGGCAGCAGA GCACAGACGA GGGCGGCGAG
    AGGCAGCCCC GGGCACAGCG CAGAGTGGAA GCCAACGGGC CGACCAAAAC CGGGGCCGAA
    TCGGCCACCA GCCCCAAGCT GCTCTATGTG GACAGAGTTG TGCAGGAAAT TCTGGAGACC
    GAACGGACTT ACGTGCAAGA TTTAAAGAGC ATCGTAGAGG ATTACCTTGA CTGTATCAGG
    GACCAAACGA AACTTCCTCT GGGGACAGAT GAAAGATCAG CCCTTTTTGG AAACATACAG
    GATATCTACC ACTTTAATAG TGAACTTCTG CAAGAGTTGG AAAACTGTGA AAATGATCCT
    GTGGCCATAG CAGAGTGCTT TGTGTCCAAG AGTGAAGAGT TCCACATTTA TACCCAGTAT
    TGCACGAACT ATCCAAGATC TGTGGCTGTG CTAACAGAGT GTATGAGGAA CAAGATGCTG
    GCCAAATTCT TCAGGGAACG GCAGGAAACT TTGAAACATT CATTGCCTCT GGGGTCCTAT
    CTCTTGAAGC CAGTTCAGCG GATTCTCAAG TATCATCTCC TTTTGCATGA AATAGAAAAT
    CACCTTGACA AGGACACAGA GGGCTACGAT GTGGTGCTTG ATGCCATAGA CACGATGCAG
    AGAGTGGCCT GGCACATCAA TGACATGAAG CGGAAGCATG AACACGCCGT CCGCCTGCAG
    GAGATACAGA GTTTGCTCAC CAACTGGAAG GGGCCAGACC TGACCAGCTA CGGGGAGCTA
    GTGCTCGAAG GGACCTTCCG CCTCCAGCGG GCCAAGAATG AGCGGACGCT CTTCCTCTTT
    GACAAGCTGC TGCTCATCAC CAAGAAGAGA GACGACACGT TTACATACAA AGCCCACATC
    CTGTGCGGCA ACCTCATGCT GGTGGAAGTG ATCCCAAAGG AGCCCCTCAG CTTCAGCGTC
    TTCCACTACA AGAACCCCAA GCTGCAGCAC ACGGTTCAGG CGAAGTCGCA GCAAGACAAG
    CGCCTGTGGG TTCTGCACCT GAAGAGGCTG ATCCTGGAGA ATCATGCGGC AAAGATTCCA
    GCTAAGGCCA AGCAAGCAAT ACTTGAAATG GACGCCATTC ATTACCCAGG CTTCTGTTAC
    AGTCCCGAGG GAGAGACTTG TGGCTCTCGA AATGGCTCTG CTCCGTATCG GCTGAGAAGG
    AAATCTGAAC CTTCCTCAAG ATCACATAAA GTTTTGAAGA CCAGTGAAAC AGCACAAGAC
    ATTCAAAAGG TTTCCAGAGA GGAAGGGTCT CCCCAGTTGA CTTCAGCAGG GCCCTCTCCT
    GCCCAGCGGA ACAGTCAGCC AAGCAGTTCG ACCATCCTGA GCGTGCTTCG TGGGGGTGGG
    GCTGTCAGAA ACATCTGGAC CGACCACCAG ATCCGCCAAG CCCTGTTTCC CAGCCGAAGG
    TCGCAGGAGA ACGAGGAGGA TGAAGACGAC TATCAGATGT TCCTGCCATC CTTCTCCTCC
    TCGGATCTGA ACTCAGCCCA GCTGTGTGAA GATGGTACTT CTAGTCGACC TTGCAGTTGG
    CACATGGGAC AAATCGAGTC CTCCGAGACC TCCAGCTCTG GCCACAGGAT TGTGCGGCGG
    GCCAGCAGTG CTGGCGAGAG CAACACGTGT CCCCCTGACA TAAGAACTAG GGACAGTGGT
    GGTTCTCAGT ACAGCTCCCG GAGGGAACTG CAGGGTGACC CTAAAACAGA GGGGCAGGAC
    GGGACAACTC CCTACGGGTC CTCTATAGAG TTGACTATAG ATGACATAGA CCATGTCTAT
    GACAACATAA GTTACGAGGA CTTGAAACTG ATGGTTGCTA AACGCGAAGA CGCTGAAGTC
    AGTCCCCAGA AACCAGTCAG GGACTCTGTT CGCCCCAAGA GCACCCCAGA GCTGGCCTTC
    TCCAAGAAGC CAGCGGGGCC TGAGGAGGAG AGCACATTGC ACCCCAGGAG AGATGGAGCC
    CTCCCGGCCG GGGAGGCCTG GAACCAAAGC ATGCACGACC TCCAGGTGAT GGAGGAGAAT
    GTCTATGACA CCATTGCGCT CCCGGAGGCG CCCTCCCGGG ACTTCAAGGG CAGCAGCCCG
    AAGCGTTCTG CAAGGAGCAC CTTCCTGGGC TTGGAGGCCG ACTTGGGGTG CTGTGATAGT
    CTGCGGGCCT CTGCTTCCCA GGACAGCCTC CAGTTCAGTG AGGACGAGGC GCCCTACCCG
    CCAGGCTCCT CTGATAACGA TTATCTGAGT TTGCTCTATA ACTCCTTCGG CTGCAACTTG
    GCCCTAGCGG ATCGGTCCAT CTCTGATAAA CTGTCTGAAG AAGTAGATGA AATCTGGAAT
    GACTTGGAAA ACTACATCAA GAAAAACGAA GTCAAAGCCA GAAACCGGCT CCTGGCCGCG
    TTTCCGGTTA GCAAAGATGA CGTGCAGGAG CGGCCGCTGG CGGGCAGTAC CCCGGAGCTG
    AGCGCGCGCT CCCCGCTCTC GCTGGCCGCG GGCCGGGCTC TCCTCGCGCC GCCGGCAGAC
    AGGCCCGGGC CAGCCCCCTG CGCCCTGGAC CGAGACCCAG CCTCCAAAGA CAGCTCCTGC
    CTGAGTCTCC ACCGGCTGTC GCTGGCCAGT GACCTGCCGC CCGCAGAGAG TCCCTATGAT
    GTGGCCGGCG GCAGCCTGTC CTCAGCGGAC CTAGAGAACC CTGAGCCCGG GCTGGACAAT
    GTAGACAAGA CCAGGAGCAG GGTTTTCCTG ATGGCCAGGC AGTACAGTCA GAAGATCAAG
    AAGGCAAATC AGCTGCTAAA AGTGAAAAAC CCAGAGCTGG AACAGACGCT CAGTAGCCAG
    CCGCAGAAGC CCGCGTCCAA GGACCTGGCG GCCATCCTAG AAGAGAAGAG GCAGGGCGGG
    CCCGCCATCG GTGCCAGGAT TGCTGAGTAT TCCCAACTGT ATGACCAGAT TGTATTTAGA
    GAGACCCCCT TTAAGACTCA GAAGGATGGC TGGGCCAGCC CTCAAGACCC CTCCAACCTC
    AGCTCTGCAT CACCTTCCCA TGCCCAGCAA GGTAGCGAGG ACTGGCTTTT GCATTCAACC
    TATAGTAATG GAGAGTTAGC TGACTTCTGT CCCTGGCCAG AACAAGACTT AAAGTCAAAA
    TATCCCATCT TAGAGATCAA TGCAAGAAGT ACCCCAAAAC AACTGTCTGC AGCTTGCTCT
    GTGCCTTCTC TTCAAACCTC CGACCCTCTG CTGGGCTTCG TGCAGAGACA CAGCGTGGTG
    GTCAGCCAGC CAAACAAAGA GAACTCCTGC CAGGGCCATC TTTACAACTC TCTGGGTCGG
    AAAGGAATCA GTGCTAAATC TCAGCCTTAT AACAGGTCCC AATCATCTTC CTCCATCCTG
    ATAAACAAGT CTGCAGACTC CATCAACTAC CCTAGTGGCG TGGCAAAGCA GCAGCCTGTC
    TCTCTACACA GAAGTTCAAG GTGGGAGAGT CACCAGGACT TGCTGCCAAG TATTGCTGAC
    TCAGGTCAAC AGGGCCCTGA AAAACGCTCA GATCTCACAC TCCAGGACTC GCAGAAAGTT
    CTGGTAGTGA ATAGAAATTT ACCTTTAAGT GCTCAAATGG CTACCCAGAA CTATTTTTCC
    AATTTCAAAG AGACTGAAGG AGACGAAGAT GACTATGTGG AGATCAAGTC AGAAGAAGAT
    GAGTCTGAGT TAGAACTGTC TCACAGTCGG AGGAGGAAAT CTGACCCAAA GATTACAGAC
    GCTGACTTTG CAGATAACGT CTGCAGCCAC ACGACTTCTT ACTCTTTGAA TAGTCCGTGC
    ACTCCAAAAA AGCCAGTAAG TGGCAAACTT GGGATTTCGC CGTATCTGAC GCCATACAAT
    GATCCCGACA AACTGAATGA CTATCTCTGG AAGGGCCCAT CTCCCAATCA GCAAAATATT
    GTGCAGTCTC TAAGGGAAAA ATTCCAGTGT CTTAGTTCAA GCAGCTTTGC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852419.1
    CDS429..4862
    Translation

    Target ORF information:

    RefSeq Version XM_024996651.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology and RhoGEF domain containing G1 (PLEKHG1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996651.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCTGTCCA CAACCATGGT GGGGGCTCTG GTCAGAGGGT GGAGAAATCG AAGCTCTCCT 
    CGAAGGGCAG AATCCAAGCT TATTATCCCT CAGAGCCAAG ATGAAATTCA GACTCGAGTT
    CTGCTGGGAG CAGAGTCAGA TGATAAAGTC AGCTCACCAC AAGATATTGA GGACCTGCCG
    GGGGAAGTTT CAGATGCGCA CCAGTTGCGT CGTGAAGTAC GGTCAGCTGT AACACAAGCT
    CGGTATCTCA GAACCACAGC CAGGCACTCC TGCTCCGGGG ACCTCGGGGC CATGGAGCTC
    TCCGACAGTG ACCGCCCGGT CAGCTTCGGC TCCACGTCGT CCTCGGCTTC CTCCCGGGAC
    AGCCATGGTT CCTTCGGCAG CAGAATGACC TTAGTTTCAA GCAGCCATCT GGGCTTGTTT
    CCTCAGGATA AGGAAGCAGG GGCCATAAAA CTGGAGCTGA TGCCAGCCAG GCCGTTTTCC
    AGCAGCGAGC TGCAGACGGA CCGTGCGGCC GGGCAGCAGA GCACAGACGA GGGCGGCGAG
    AGGCAGCCCC GGGCACAGCG CAGAGTGGAA GCCAACGGGC CGACCAAAAC CGGGGCCGAA
    TCGGCCACCA GCCCCAAGCT GCTCTATGTG GACAGAGTTG TGCAGGAAAT TCTGGAGACC
    GAACGGACTT ACGTGCAAGA TTTAAAGAGC ATCGTAGAGG ATTACCTTGA CTGTATCAGG
    GACCAAACGA AACTTCCTCT GGGGACAGAT GAAAGATCAG CCCTTTTTGG AAACATACAG
    GATATCTACC ACTTTAATAG TGAACTTCTG CAAGAGTTGG AAAACTGTGA AAATGATCCT
    GTGGCCATAG CAGAGTGCTT TGTGTCCAAG AGTGAAGAGT TCCACATTTA TACCCAGTAT
    TGCACGAACT ATCCAAGATC TGTGGCTGTG CTAACAGAGT GTATGAGGAA CAAGATGCTG
    GCCAAATTCT TCAGGGAACG GCAGGAAACT TTGAAACATT CATTGCCTCT GGGGTCCTAT
    CTCTTGAAGC CAGTTCAGCG GATTCTCAAG TATCATCTCC TTTTGCATGA AATAGAAAAT
    CACCTTGACA AGGACACAGA GGGCTACGAT GTGGTGCTTG ATGCCATAGA CACGATGCAG
    AGAGTGGCCT GGCACATCAA TGACATGAAG CGGAAGCATG AACACGCCGT CCGCCTGCAG
    GAGATACAGA GTTTGCTCAC CAACTGGAAG GGGCCAGACC TGACCAGCTA CGGGGAGCTA
    GTGCTCGAAG GGACCTTCCG CCTCCAGCGG GCCAAGAATG AGCGGACGCT CTTCCTCTTT
    GACAAGCTGC TGCTCATCAC CAAGAAGAGA GACGACACGT TTACATACAA AGCCCACATC
    CTGTGCGGCA ACCTCATGCT GGTGGAAGTG ATCCCAAAGG AGCCCCTCAG CTTCAGCGTC
    TTCCACTACA AGAACCCCAA GCTGCAGCAC ACGGTTCAGG CGAAGTCGCA GCAAGACAAG
    CGCCTGTGGG TTCTGCACCT GAAGAGGCTG ATCCTGGAGA ATCATGCGGC AAAGATTCCA
    GCTAAGGCCA AGCAAGCAAT ACTTGAAATG GACGCCATTC ATTACCCAGG CTTCTGTTAC
    AGTCCCGAGG GAGAGACTTG TGGCTCTCGA AATGGCTCTG CTCCGTATCG GCTGAGAAGG
    AAATCTGAAC CTTCCTCAAG ATCACATAAA GTTTTGAAGA CCAGTGAAAC AGCACAAGAC
    ATTCAAAAGG TTTCCAGAGA GGAAGGGTCT CCCCAGTTGA CTTCAGCAGG GCCCTCTCCT
    GCCCAGCGGA ACAGTCAGCC AAGCAGTTCG ACCATCCTGA GCGTGCTTCG TGGGGGTGGG
    GCTGTCAGAA ACATCTGGAC CGACCACCAG ATCCGCCAAG CCCTGTTTCC CAGCCGAAGG
    TCGCAGGAGA ACGAGGAGGA TGAAGACGAC TATCAGATGT TCCTGCCATC CTTCTCCTCC
    TCGGATCTGA ACTCAGCCCA GCTGTGTGAA GATGGTACTT CTAGTCGACC TTGCAGTTGG
    CACATGGGAC AAATCGAGTC CTCCGAGACC TCCAGCTCTG GCCACAGGAT TGTGCGGCGG
    GCCAGCAGTG CTGGCGAGAG CAACACGTGT CCCCCTGACA TAAGAACTAG GGACAGTGGT
    GGTTCTCAGT ACAGCTCCCG GAGGGAACTG CAGGGTGACC CTAAAACAGA GGGGCAGGAC
    GGGACAACTC CCTACGGGTC CTCTATAGAG TTGACTATAG ATGACATAGA CCATGTCTAT
    GACAACATAA GTTACGAGGA CTTGAAACTG ATGGTTGCTA AACGCGAAGA CGCTGAAGTC
    AGTCCCCAGA AACCAGTCAG GGACTCTGTT CGCCCCAAGA GCACCCCAGA GCTGGCCTTC
    TCCAAGAAGC CAGCGGGGCC TGAGGAGGAG AGCACATTGC ACCCCAGGAG AGATGGAGCC
    CTCCCGGCCG GGGAGGCCTG GAACCAAAGC ATGCACGACC TCCAGGTGAT GGAGGAGAAT
    GTCTATGACA CCATTGCGCT CCCGGAGGCG CCCTCCCGGG ACTTCAAGGG CAGCAGCCCG
    AAGCGTTCTG CAAGGAGCAC CTTCCTGGGC TTGGAGGCCG ACTTGGGGTG CTGTGATAGT
    CTGCGGGCCT CTGCTTCCCA GGACAGCCTC CAGTTCAGTG AGGACGAGGC GCCCTACCCG
    CCAGGCTCCT CTGATAACGA TTATCTGAGT TTGCTCTATA ACTCCTTCGG CTGCAACTTG
    GCCCTAGCGG ATCGGTCCAT CTCTGATAAA CTGTCTGAAG AAGTAGATGA AATCTGGAAT
    GACTTGGAAA ACTACATCAA GAAAAACGAA GTCAAAGCCA GAAACCGGCT CCTGGCCGCG
    TTTCCGGTTA GCAAAGATGA CGTGCAGGAG CGGCCGCTGG CGGGCAGTAC CCCGGAGCTG
    AGCGCGCGCT CCCCGCTCTC GCTGGCCGCG GGCCGGGCTC TCCTCGCGCC GCCGGCAGAC
    AGGCCCGGGC CAGCCCCCTG CGCCCTGGAC CGAGACCCAG CCTCCAAAGA CAGCTCCTGC
    CTGAGTCTCC ACCGGCTGTC GCTGGCCAGT GACCTGCCGC CCGCAGAGAG TCCCTATGAT
    GTGGCCGGCG GCAGCCTGTC CTCAGCGGAC CTAGAGAACC CTGAGCCCGG GCTGGACAAT
    GTAGACAAGA CCAGGAGCAG GGTTTTCCTG ATGGCCAGGC AGTACAGTCA GAAGATCAAG
    AAGGCAAATC AGCTGCTAAA AGTGAAAAAC CCAGAGCTGG AACAGACGCT CAGTAGCCAG
    CCGCAGAAGC CCGCGTCCAA GGACCTGGCG GCCATCCTAG AAGAGAAGAG GCAGGGCGGG
    CCCGCCATCG GTGCCAGGAT TGCTGAGTAT TCCCAACTGT ATGACCAGAT TGTATTTAGA
    GAGACCCCCT TTAAGACTCA GAAGGATGGC TGGGCCAGCC CTCAAGACCC CTCCAACCTC
    AGCTCTGCAT CACCTTCCCA TGCCCAGCAA GGTAGCGAGG ACTGGCTTTT GCATTCAACC
    TATAGTAATG GAGAGTTAGC TGACTTCTGT CCCTGGCCAG AACAAGACTT AAAGTCAAAA
    TATCCCATCT TAGAGATCAA TGCAAGAAGT ACCCCAAAAC AACTGTCTGC AGCTTGCTCT
    GTGCCTTCTC TTCAAACCTC CGACCCTCTG CTGGGCTTCG TGCAGAGACA CAGCGTGGTG
    GTCAGCCAGC CAAACAAAGA GAACTCCTGC CAGGGCCATC TTTACAACTC TCTGGGTCGG
    AAAGGAATCA GTGCTAAATC TCAGCCTTAT AACAGGTCCC AATCATCTTC CTCCATCCTG
    ATAAACAAGT CTGCAGACTC CATCAACTAC CCTAGTGGCG TGGCAAAGCA GCAGCCTGTC
    TCTCTACACA GAAGTTCAAG GTGGGAGAGT CACCAGGACT TGCTGCCAAG TATTGCTGAC
    TCAGGTCAAC AGGGCCCTGA AAAACGCTCA GATCTCACAC TCCAGGACTC GCAGAAAGTT
    CTGGTAGTGA ATAGAAATTT ACCTTTAAGT GCTCAAATGG CTACCCAGAA CTATTTTTCC
    AATTTCAAAG AGACTGAAGG AGACGAAGAT GACTATGTGG AGATCAAGTC AGAAGAAGAT
    GAGTCTGAGT TAGAACTGTC TCACAGTCGG AGGAGGAAAT CTGACCCAAA GATTACAGAC
    GCTGACTTTG CAGATAACGT CTGCAGCCAC ACGACTTCTT ACTCTTTGAA TAGTCCGTGC
    ACTCCAAAAA AGCCAGTAAG TGGCAAACTT GGGATTTCGC CGTATCTGAC GCCATACAAT
    GATCCCGACA AACTGAATGA CTATCTCTGG AAGGGCCCAT CTCCCAATCA GCAAAATATT
    GTGCAGTCTC TAAGGGAAAA ATTCCAGTGT CTTAGTTCAA GCAGCTTTGC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237776
    Clone ID Related Accession (Same CDS sequence) XM_024996652.1
    Accession Version XM_024996652.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4359bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pleckstrin homology domain-containing family G member 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCTGTCCA CAACCATGGT GGGGGCTCTG GTCAGAGGGT GGAGAAATCG AAGCTCTCCT 
    CGAAGGGCAG AATCCAAGCT TATTATCCCT CAGAGCCAAG ATGAAATTCA GACTCGAGAT
    GCGCACCAGT TGCGTCGTGA AGTACGGTCA GCTGTAACAC AAGCTCGGTA TCTCAGAACC
    ACAGCCAGGC ACTCCTGCTC CGGGGACCTC GGGGCCATGG AGCTCTCCGA CAGTGACCGC
    CCGGTCAGCT TCGGCTCCAC GTCGTCCTCG GCTTCCTCCC GGGACAGCCA TGGTTCCTTC
    GGCAGCAGAA TGACCTTAGT TTCAAGCAGC CATCTGGGCT TGTTTCCTCA GGATAAGGAA
    GCAGGGGCCA TAAAACTGGA GCTGATGCCA GCCAGGCCGT TTTCCAGCAG CGAGCTGCAG
    ACGGACCGTG CGGCCGGGCA GCAGAGCACA GACGAGGGCG GCGAGAGGCA GCCCCGGGCA
    CAGCGCAGAG TGGAAGCCAA CGGGCCGACC AAAACCGGGG CCGAATCGGC CACCAGCCCC
    AAGCTGCTCT ATGTGGACAG AGTTGTGCAG GAAATTCTGG AGACCGAACG GACTTACGTG
    CAAGATTTAA AGAGCATCGT AGAGGATTAC CTTGACTGTA TCAGGGACCA AACGAAACTT
    CCTCTGGGGA CAGATGAAAG ATCAGCCCTT TTTGGAAACA TACAGGATAT CTACCACTTT
    AATAGTGAAC TTCTGCAAGA GTTGGAAAAC TGTGAAAATG ATCCTGTGGC CATAGCAGAG
    TGCTTTGTGT CCAAGAGTGA AGAGTTCCAC ATTTATACCC AGTATTGCAC GAACTATCCA
    AGATCTGTGG CTGTGCTAAC AGAGTGTATG AGGAACAAGA TGCTGGCCAA ATTCTTCAGG
    GAACGGCAGG AAACTTTGAA ACATTCATTG CCTCTGGGGT CCTATCTCTT GAAGCCAGTT
    CAGCGGATTC TCAAGTATCA TCTCCTTTTG CATGAAATAG AAAATCACCT TGACAAGGAC
    ACAGAGGGCT ACGATGTGGT GCTTGATGCC ATAGACACGA TGCAGAGAGT GGCCTGGCAC
    ATCAATGACA TGAAGCGGAA GCATGAACAC GCCGTCCGCC TGCAGGAGAT ACAGAGTTTG
    CTCACCAACT GGAAGGGGCC AGACCTGACC AGCTACGGGG AGCTAGTGCT CGAAGGGACC
    TTCCGCCTCC AGCGGGCCAA GAATGAGCGG ACGCTCTTCC TCTTTGACAA GCTGCTGCTC
    ATCACCAAGA AGAGAGACGA CACGTTTACA TACAAAGCCC ACATCCTGTG CGGCAACCTC
    ATGCTGGTGG AAGTGATCCC AAAGGAGCCC CTCAGCTTCA GCGTCTTCCA CTACAAGAAC
    CCCAAGCTGC AGCACACGGT TCAGGCGAAG TCGCAGCAAG ACAAGCGCCT GTGGGTTCTG
    CACCTGAAGA GGCTGATCCT GGAGAATCAT GCGGCAAAGA TTCCAGCTAA GGCCAAGCAA
    GCAATACTTG AAATGGACGC CATTCATTAC CCAGGCTTCT GTTACAGTCC CGAGGGAGAG
    ACTTGTGGCT CTCGAAATGG CTCTGCTCCG TATCGGCTGA GAAGGAAATC TGAACCTTCC
    TCAAGATCAC ATAAAGTTTT GAAGACCAGT GAAACAGCAC AAGACATTCA AAAGGTTTCC
    AGAGAGGAAG GGTCTCCCCA GTTGACTTCA GCAGGGCCCT CTCCTGCCCA GCGGAACAGT
    CAGCCAAGCA GTTCGACCAT CCTGAGCGTG CTTCGTGGGG GTGGGGCTGT CAGAAACATC
    TGGACCGACC ACCAGATCCG CCAAGCCCTG TTTCCCAGCC GAAGGTCGCA GGAGAACGAG
    GAGGATGAAG ACGACTATCA GATGTTCCTG CCATCCTTCT CCTCCTCGGA TCTGAACTCA
    GCCCAGCTGT GTGAAGATGG TACTTCTAGT CGACCTTGCA GTTGGCACAT GGGACAAATC
    GAGTCCTCCG AGACCTCCAG CTCTGGCCAC AGGATTGTGC GGCGGGCCAG CAGTGCTGGC
    GAGAGCAACA CGTGTCCCCC TGACATAAGA ACTAGGGACA GTGGTGGTTC TCAGTACAGC
    TCCCGGAGGG AACTGCAGGG TGACCCTAAA ACAGAGGGGC AGGACGGGAC AACTCCCTAC
    GGGTCCTCTA TAGAGTTGAC TATAGATGAC ATAGACCATG TCTATGACAA CATAAGTTAC
    GAGGACTTGA AACTGATGGT TGCTAAACGC GAAGACGCTG AAGTCAGTCC CCAGAAACCA
    GTCAGGGACT CTGTTCGCCC CAAGAGCACC CCAGAGCTGG CCTTCTCCAA GAAGCCAGCG
    GGGCCTGAGG AGGAGAGCAC ATTGCACCCC AGGAGAGATG GAGCCCTCCC GGCCGGGGAG
    GCCTGGAACC AAAGCATGCA CGACCTCCAG GTGATGGAGG AGAATGTCTA TGACACCATT
    GCGCTCCCGG AGGCGCCCTC CCGGGACTTC AAGGGCAGCA GCCCGAAGCG TTCTGCAAGG
    AGCACCTTCC TGGGCTTGGA GGCCGACTTG GGGTGCTGTG ATAGTCTGCG GGCCTCTGCT
    TCCCAGGACA GCCTCCAGTT CAGTGAGGAC GAGGCGCCCT ACCCGCCAGG CTCCTCTGAT
    AACGATTATC TGAGTTTGCT CTATAACTCC TTCGGCTGCA ACTTGGCCCT AGCGGATCGG
    TCCATCTCTG ATAAACTGTC TGAAGAAGTA GATGAAATCT GGAATGACTT GGAAAACTAC
    ATCAAGAAAA ACGAAGTCAA AGCCAGAAAC CGGCTCCTGG CCGCGTTTCC GGTTAGCAAA
    GATGACGTGC AGGAGCGGCC GCTGGCGGGC AGTACCCCGG AGCTGAGCGC GCGCTCCCCG
    CTCTCGCTGG CCGCGGGCCG GGCTCTCCTC GCGCCGCCGG CAGACAGGCC CGGGCCAGCC
    CCCTGCGCCC TGGACCGAGA CCCAGCCTCC AAAGACAGCT CCTGCCTGAG TCTCCACCGG
    CTGTCGCTGG CCAGTGACCT GCCGCCCGCA GAGAGTCCCT ATGATGTGGC CGGCGGCAGC
    CTGTCCTCAG CGGACCTAGA GAACCCTGAG CCCGGGCTGG ACAATGTAGA CAAGACCAGG
    AGCAGGGTTT TCCTGATGGC CAGGCAGTAC AGTCAGAAGA TCAAGAAGGC AAATCAGCTG
    CTAAAAGTGA AAAACCCAGA GCTGGAACAG ACGCTCAGTA GCCAGCCGCA GAAGCCCGCG
    TCCAAGGACC TGGCGGCCAT CCTAGAAGAG AAGAGGCAGG GCGGGCCCGC CATCGGTGCC
    AGGATTGCTG AGTATTCCCA ACTGTATGAC CAGATTGTAT TTAGAGAGAC CCCCTTTAAG
    ACTCAGAAGG ATGGCTGGGC CAGCCCTCAA GACCCCTCCA ACCTCAGCTC TGCATCACCT
    TCCCATGCCC AGCAAGGTAG CGAGGACTGG CTTTTGCATT CAACCTATAG TAATGGAGAG
    TTAGCTGACT TCTGTCCCTG GCCAGAACAA GACTTAAAGT CAAAATATCC CATCTTAGAG
    ATCAATGCAA GAAGTACCCC AAAACAACTG TCTGCAGCTT GCTCTGTGCC TTCTCTTCAA
    ACCTCCGACC CTCTGCTGGG CTTCGTGCAG AGACACAGCG TGGTGGTCAG CCAGCCAAAC
    AAAGAGAACT CCTGCCAGGG CCATCTTTAC AACTCTCTGG GTCGGAAAGG AATCAGTGCT
    AAATCTCAGC CTTATAACAG GTCCCAATCA TCTTCCTCCA TCCTGATAAA CAAGTCTGCA
    GACTCCATCA ACTACCCTAG TGGCGTGGCA AAGCAGCAGC CTGTCTCTCT ACACAGAAGT
    TCAAGGTGGG AGAGTCACCA GGACTTGCTG CCAAGTATTG CTGACTCAGG TCAACAGGGC
    CCTGAAAAAC GCTCAGATCT CACACTCCAG GACTCGCAGA AAGTTCTGGT AGTGAATAGA
    AATTTACCTT TAAGTGCTCA AATGGCTACC CAGAACTATT TTTCCAATTT CAAAGAGACT
    GAAGGAGACG AAGATGACTA TGTGGAGATC AAGTCAGAAG AAGATGAGTC TGAGTTAGAA
    CTGTCTCACA GTCGGAGGAG GAAATCTGAC CCAAAGATTA CAGACGCTGA CTTTGCAGAT
    AACGTCTGCA GCCACACGAC TTCTTACTCT TTGAATAGTC CGTGCACTCC AAAAAAGCCA
    GTAAGTGGCA AACTTGGGAT TTCGCCGTAT CTGACGCCAT ACAATGATCC CGACAAACTG
    AATGACTATC TCTGGAAGGG CCCATCTCCC AATCAGCAAA ATATTGTGCA GTCTCTAAGG
    GAAAAATTCC AGTGTCTTAG TTCAAGCAGC TTTGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852420.1
    CDS429..4787
    Translation

    Target ORF information:

    RefSeq Version XM_024996652.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology and RhoGEF domain containing G1 (PLEKHG1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996652.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCTGTCCA CAACCATGGT GGGGGCTCTG GTCAGAGGGT GGAGAAATCG AAGCTCTCCT 
    CGAAGGGCAG AATCCAAGCT TATTATCCCT CAGAGCCAAG ATGAAATTCA GACTCGAGAT
    GCGCACCAGT TGCGTCGTGA AGTACGGTCA GCTGTAACAC AAGCTCGGTA TCTCAGAACC
    ACAGCCAGGC ACTCCTGCTC CGGGGACCTC GGGGCCATGG AGCTCTCCGA CAGTGACCGC
    CCGGTCAGCT TCGGCTCCAC GTCGTCCTCG GCTTCCTCCC GGGACAGCCA TGGTTCCTTC
    GGCAGCAGAA TGACCTTAGT TTCAAGCAGC CATCTGGGCT TGTTTCCTCA GGATAAGGAA
    GCAGGGGCCA TAAAACTGGA GCTGATGCCA GCCAGGCCGT TTTCCAGCAG CGAGCTGCAG
    ACGGACCGTG CGGCCGGGCA GCAGAGCACA GACGAGGGCG GCGAGAGGCA GCCCCGGGCA
    CAGCGCAGAG TGGAAGCCAA CGGGCCGACC AAAACCGGGG CCGAATCGGC CACCAGCCCC
    AAGCTGCTCT ATGTGGACAG AGTTGTGCAG GAAATTCTGG AGACCGAACG GACTTACGTG
    CAAGATTTAA AGAGCATCGT AGAGGATTAC CTTGACTGTA TCAGGGACCA AACGAAACTT
    CCTCTGGGGA CAGATGAAAG ATCAGCCCTT TTTGGAAACA TACAGGATAT CTACCACTTT
    AATAGTGAAC TTCTGCAAGA GTTGGAAAAC TGTGAAAATG ATCCTGTGGC CATAGCAGAG
    TGCTTTGTGT CCAAGAGTGA AGAGTTCCAC ATTTATACCC AGTATTGCAC GAACTATCCA
    AGATCTGTGG CTGTGCTAAC AGAGTGTATG AGGAACAAGA TGCTGGCCAA ATTCTTCAGG
    GAACGGCAGG AAACTTTGAA ACATTCATTG CCTCTGGGGT CCTATCTCTT GAAGCCAGTT
    CAGCGGATTC TCAAGTATCA TCTCCTTTTG CATGAAATAG AAAATCACCT TGACAAGGAC
    ACAGAGGGCT ACGATGTGGT GCTTGATGCC ATAGACACGA TGCAGAGAGT GGCCTGGCAC
    ATCAATGACA TGAAGCGGAA GCATGAACAC GCCGTCCGCC TGCAGGAGAT ACAGAGTTTG
    CTCACCAACT GGAAGGGGCC AGACCTGACC AGCTACGGGG AGCTAGTGCT CGAAGGGACC
    TTCCGCCTCC AGCGGGCCAA GAATGAGCGG ACGCTCTTCC TCTTTGACAA GCTGCTGCTC
    ATCACCAAGA AGAGAGACGA CACGTTTACA TACAAAGCCC ACATCCTGTG CGGCAACCTC
    ATGCTGGTGG AAGTGATCCC AAAGGAGCCC CTCAGCTTCA GCGTCTTCCA CTACAAGAAC
    CCCAAGCTGC AGCACACGGT TCAGGCGAAG TCGCAGCAAG ACAAGCGCCT GTGGGTTCTG
    CACCTGAAGA GGCTGATCCT GGAGAATCAT GCGGCAAAGA TTCCAGCTAA GGCCAAGCAA
    GCAATACTTG AAATGGACGC CATTCATTAC CCAGGCTTCT GTTACAGTCC CGAGGGAGAG
    ACTTGTGGCT CTCGAAATGG CTCTGCTCCG TATCGGCTGA GAAGGAAATC TGAACCTTCC
    TCAAGATCAC ATAAAGTTTT GAAGACCAGT GAAACAGCAC AAGACATTCA AAAGGTTTCC
    AGAGAGGAAG GGTCTCCCCA GTTGACTTCA GCAGGGCCCT CTCCTGCCCA GCGGAACAGT
    CAGCCAAGCA GTTCGACCAT CCTGAGCGTG CTTCGTGGGG GTGGGGCTGT CAGAAACATC
    TGGACCGACC ACCAGATCCG CCAAGCCCTG TTTCCCAGCC GAAGGTCGCA GGAGAACGAG
    GAGGATGAAG ACGACTATCA GATGTTCCTG CCATCCTTCT CCTCCTCGGA TCTGAACTCA
    GCCCAGCTGT GTGAAGATGG TACTTCTAGT CGACCTTGCA GTTGGCACAT GGGACAAATC
    GAGTCCTCCG AGACCTCCAG CTCTGGCCAC AGGATTGTGC GGCGGGCCAG CAGTGCTGGC
    GAGAGCAACA CGTGTCCCCC TGACATAAGA ACTAGGGACA GTGGTGGTTC TCAGTACAGC
    TCCCGGAGGG AACTGCAGGG TGACCCTAAA ACAGAGGGGC AGGACGGGAC AACTCCCTAC
    GGGTCCTCTA TAGAGTTGAC TATAGATGAC ATAGACCATG TCTATGACAA CATAAGTTAC
    GAGGACTTGA AACTGATGGT TGCTAAACGC GAAGACGCTG AAGTCAGTCC CCAGAAACCA
    GTCAGGGACT CTGTTCGCCC CAAGAGCACC CCAGAGCTGG CCTTCTCCAA GAAGCCAGCG
    GGGCCTGAGG AGGAGAGCAC ATTGCACCCC AGGAGAGATG GAGCCCTCCC GGCCGGGGAG
    GCCTGGAACC AAAGCATGCA CGACCTCCAG GTGATGGAGG AGAATGTCTA TGACACCATT
    GCGCTCCCGG AGGCGCCCTC CCGGGACTTC AAGGGCAGCA GCCCGAAGCG TTCTGCAAGG
    AGCACCTTCC TGGGCTTGGA GGCCGACTTG GGGTGCTGTG ATAGTCTGCG GGCCTCTGCT
    TCCCAGGACA GCCTCCAGTT CAGTGAGGAC GAGGCGCCCT ACCCGCCAGG CTCCTCTGAT
    AACGATTATC TGAGTTTGCT CTATAACTCC TTCGGCTGCA ACTTGGCCCT AGCGGATCGG
    TCCATCTCTG ATAAACTGTC TGAAGAAGTA GATGAAATCT GGAATGACTT GGAAAACTAC
    ATCAAGAAAA ACGAAGTCAA AGCCAGAAAC CGGCTCCTGG CCGCGTTTCC GGTTAGCAAA
    GATGACGTGC AGGAGCGGCC GCTGGCGGGC AGTACCCCGG AGCTGAGCGC GCGCTCCCCG
    CTCTCGCTGG CCGCGGGCCG GGCTCTCCTC GCGCCGCCGG CAGACAGGCC CGGGCCAGCC
    CCCTGCGCCC TGGACCGAGA CCCAGCCTCC AAAGACAGCT CCTGCCTGAG TCTCCACCGG
    CTGTCGCTGG CCAGTGACCT GCCGCCCGCA GAGAGTCCCT ATGATGTGGC CGGCGGCAGC
    CTGTCCTCAG CGGACCTAGA GAACCCTGAG CCCGGGCTGG ACAATGTAGA CAAGACCAGG
    AGCAGGGTTT TCCTGATGGC CAGGCAGTAC AGTCAGAAGA TCAAGAAGGC AAATCAGCTG
    CTAAAAGTGA AAAACCCAGA GCTGGAACAG ACGCTCAGTA GCCAGCCGCA GAAGCCCGCG
    TCCAAGGACC TGGCGGCCAT CCTAGAAGAG AAGAGGCAGG GCGGGCCCGC CATCGGTGCC
    AGGATTGCTG AGTATTCCCA ACTGTATGAC CAGATTGTAT TTAGAGAGAC CCCCTTTAAG
    ACTCAGAAGG ATGGCTGGGC CAGCCCTCAA GACCCCTCCA ACCTCAGCTC TGCATCACCT
    TCCCATGCCC AGCAAGGTAG CGAGGACTGG CTTTTGCATT CAACCTATAG TAATGGAGAG
    TTAGCTGACT TCTGTCCCTG GCCAGAACAA GACTTAAAGT CAAAATATCC CATCTTAGAG
    ATCAATGCAA GAAGTACCCC AAAACAACTG TCTGCAGCTT GCTCTGTGCC TTCTCTTCAA
    ACCTCCGACC CTCTGCTGGG CTTCGTGCAG AGACACAGCG TGGTGGTCAG CCAGCCAAAC
    AAAGAGAACT CCTGCCAGGG CCATCTTTAC AACTCTCTGG GTCGGAAAGG AATCAGTGCT
    AAATCTCAGC CTTATAACAG GTCCCAATCA TCTTCCTCCA TCCTGATAAA CAAGTCTGCA
    GACTCCATCA ACTACCCTAG TGGCGTGGCA AAGCAGCAGC CTGTCTCTCT ACACAGAAGT
    TCAAGGTGGG AGAGTCACCA GGACTTGCTG CCAAGTATTG CTGACTCAGG TCAACAGGGC
    CCTGAAAAAC GCTCAGATCT CACACTCCAG GACTCGCAGA AAGTTCTGGT AGTGAATAGA
    AATTTACCTT TAAGTGCTCA AATGGCTACC CAGAACTATT TTTCCAATTT CAAAGAGACT
    GAAGGAGACG AAGATGACTA TGTGGAGATC AAGTCAGAAG AAGATGAGTC TGAGTTAGAA
    CTGTCTCACA GTCGGAGGAG GAAATCTGAC CCAAAGATTA CAGACGCTGA CTTTGCAGAT
    AACGTCTGCA GCCACACGAC TTCTTACTCT TTGAATAGTC CGTGCACTCC AAAAAAGCCA
    GTAAGTGGCA AACTTGGGAT TTCGCCGTAT CTGACGCCAT ACAATGATCC CGACAAACTG
    AATGACTATC TCTGGAAGGG CCCATCTCCC AATCAGCAAA ATATTGTGCA GTCTCTAAGG
    GAAAAATTCC AGTGTCTTAG TTCAAGCAGC TTTGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237777
    Clone ID Related Accession (Same CDS sequence) XM_024996653.1
    Accession Version XM_024996653.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4311bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pleckstrin homology domain-containing family G member 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGTGTCTGG GGAACCGATG CTCGACACCC TTGGTCCTTC CTGCTGGAGC AGCCGAGCCC 
    AACCGCCTCG ATGCGCACCA GTTGCGTCGT GAAGTACGGT CAGCTGTAAC ACAAGCTCGG
    TATCTCAGAA CCACAGCCAG GCACTCCTGC TCCGGGGACC TCGGGGCCAT GGAGCTCTCC
    GACAGTGACC GCCCGGTCAG CTTCGGCTCC ACGTCGTCCT CGGCTTCCTC CCGGGACAGC
    CATGGTTCCT TCGGCAGCAG AATGACCTTA GTTTCAAGCA GCCATCTGGG CTTGTTTCCT
    CAGGATAAGG AAGCAGGGGC CATAAAACTG GAGCTGATGC CAGCCAGGCC GTTTTCCAGC
    AGCGAGCTGC AGACGGACCG TGCGGCCGGG CAGCAGAGCA CAGACGAGGG CGGCGAGAGG
    CAGCCCCGGG CACAGCGCAG AGTGGAAGCC AACGGGCCGA CCAAAACCGG GGCCGAATCG
    GCCACCAGCC CCAAGCTGCT CTATGTGGAC AGAGTTGTGC AGGAAATTCT GGAGACCGAA
    CGGACTTACG TGCAAGATTT AAAGAGCATC GTAGAGGATT ACCTTGACTG TATCAGGGAC
    CAAACGAAAC TTCCTCTGGG GACAGATGAA AGATCAGCCC TTTTTGGAAA CATACAGGAT
    ATCTACCACT TTAATAGTGA ACTTCTGCAA GAGTTGGAAA ACTGTGAAAA TGATCCTGTG
    GCCATAGCAG AGTGCTTTGT GTCCAAGAGT GAAGAGTTCC ACATTTATAC CCAGTATTGC
    ACGAACTATC CAAGATCTGT GGCTGTGCTA ACAGAGTGTA TGAGGAACAA GATGCTGGCC
    AAATTCTTCA GGGAACGGCA GGAAACTTTG AAACATTCAT TGCCTCTGGG GTCCTATCTC
    TTGAAGCCAG TTCAGCGGAT TCTCAAGTAT CATCTCCTTT TGCATGAAAT AGAAAATCAC
    CTTGACAAGG ACACAGAGGG CTACGATGTG GTGCTTGATG CCATAGACAC GATGCAGAGA
    GTGGCCTGGC ACATCAATGA CATGAAGCGG AAGCATGAAC ACGCCGTCCG CCTGCAGGAG
    ATACAGAGTT TGCTCACCAA CTGGAAGGGG CCAGACCTGA CCAGCTACGG GGAGCTAGTG
    CTCGAAGGGA CCTTCCGCCT CCAGCGGGCC AAGAATGAGC GGACGCTCTT CCTCTTTGAC
    AAGCTGCTGC TCATCACCAA GAAGAGAGAC GACACGTTTA CATACAAAGC CCACATCCTG
    TGCGGCAACC TCATGCTGGT GGAAGTGATC CCAAAGGAGC CCCTCAGCTT CAGCGTCTTC
    CACTACAAGA ACCCCAAGCT GCAGCACACG GTTCAGGCGA AGTCGCAGCA AGACAAGCGC
    CTGTGGGTTC TGCACCTGAA GAGGCTGATC CTGGAGAATC ATGCGGCAAA GATTCCAGCT
    AAGGCCAAGC AAGCAATACT TGAAATGGAC GCCATTCATT ACCCAGGCTT CTGTTACAGT
    CCCGAGGGAG AGACTTGTGG CTCTCGAAAT GGCTCTGCTC CGTATCGGCT GAGAAGGAAA
    TCTGAACCTT CCTCAAGATC ACATAAAGTT TTGAAGACCA GTGAAACAGC ACAAGACATT
    CAAAAGGTTT CCAGAGAGGA AGGGTCTCCC CAGTTGACTT CAGCAGGGCC CTCTCCTGCC
    CAGCGGAACA GTCAGCCAAG CAGTTCGACC ATCCTGAGCG TGCTTCGTGG GGGTGGGGCT
    GTCAGAAACA TCTGGACCGA CCACCAGATC CGCCAAGCCC TGTTTCCCAG CCGAAGGTCG
    CAGGAGAACG AGGAGGATGA AGACGACTAT CAGATGTTCC TGCCATCCTT CTCCTCCTCG
    GATCTGAACT CAGCCCAGCT GTGTGAAGAT GGTACTTCTA GTCGACCTTG CAGTTGGCAC
    ATGGGACAAA TCGAGTCCTC CGAGACCTCC AGCTCTGGCC ACAGGATTGT GCGGCGGGCC
    AGCAGTGCTG GCGAGAGCAA CACGTGTCCC CCTGACATAA GAACTAGGGA CAGTGGTGGT
    TCTCAGTACA GCTCCCGGAG GGAACTGCAG GGTGACCCTA AAACAGAGGG GCAGGACGGG
    ACAACTCCCT ACGGGTCCTC TATAGAGTTG ACTATAGATG ACATAGACCA TGTCTATGAC
    AACATAAGTT ACGAGGACTT GAAACTGATG GTTGCTAAAC GCGAAGACGC TGAAGTCAGT
    CCCCAGAAAC CAGTCAGGGA CTCTGTTCGC CCCAAGAGCA CCCCAGAGCT GGCCTTCTCC
    AAGAAGCCAG CGGGGCCTGA GGAGGAGAGC ACATTGCACC CCAGGAGAGA TGGAGCCCTC
    CCGGCCGGGG AGGCCTGGAA CCAAAGCATG CACGACCTCC AGGTGATGGA GGAGAATGTC
    TATGACACCA TTGCGCTCCC GGAGGCGCCC TCCCGGGACT TCAAGGGCAG CAGCCCGAAG
    CGTTCTGCAA GGAGCACCTT CCTGGGCTTG GAGGCCGACT TGGGGTGCTG TGATAGTCTG
    CGGGCCTCTG CTTCCCAGGA CAGCCTCCAG TTCAGTGAGG ACGAGGCGCC CTACCCGCCA
    GGCTCCTCTG ATAACGATTA TCTGAGTTTG CTCTATAACT CCTTCGGCTG CAACTTGGCC
    CTAGCGGATC GGTCCATCTC TGATAAACTG TCTGAAGAAG TAGATGAAAT CTGGAATGAC
    TTGGAAAACT ACATCAAGAA AAACGAAGTC AAAGCCAGAA ACCGGCTCCT GGCCGCGTTT
    CCGGTTAGCA AAGATGACGT GCAGGAGCGG CCGCTGGCGG GCAGTACCCC GGAGCTGAGC
    GCGCGCTCCC CGCTCTCGCT GGCCGCGGGC CGGGCTCTCC TCGCGCCGCC GGCAGACAGG
    CCCGGGCCAG CCCCCTGCGC CCTGGACCGA GACCCAGCCT CCAAAGACAG CTCCTGCCTG
    AGTCTCCACC GGCTGTCGCT GGCCAGTGAC CTGCCGCCCG CAGAGAGTCC CTATGATGTG
    GCCGGCGGCA GCCTGTCCTC AGCGGACCTA GAGAACCCTG AGCCCGGGCT GGACAATGTA
    GACAAGACCA GGAGCAGGGT TTTCCTGATG GCCAGGCAGT ACAGTCAGAA GATCAAGAAG
    GCAAATCAGC TGCTAAAAGT GAAAAACCCA GAGCTGGAAC AGACGCTCAG TAGCCAGCCG
    CAGAAGCCCG CGTCCAAGGA CCTGGCGGCC ATCCTAGAAG AGAAGAGGCA GGGCGGGCCC
    GCCATCGGTG CCAGGATTGC TGAGTATTCC CAACTGTATG ACCAGATTGT ATTTAGAGAG
    ACCCCCTTTA AGACTCAGAA GGATGGCTGG GCCAGCCCTC AAGACCCCTC CAACCTCAGC
    TCTGCATCAC CTTCCCATGC CCAGCAAGGT AGCGAGGACT GGCTTTTGCA TTCAACCTAT
    AGTAATGGAG AGTTAGCTGA CTTCTGTCCC TGGCCAGAAC AAGACTTAAA GTCAAAATAT
    CCCATCTTAG AGATCAATGC AAGAAGTACC CCAAAACAAC TGTCTGCAGC TTGCTCTGTG
    CCTTCTCTTC AAACCTCCGA CCCTCTGCTG GGCTTCGTGC AGAGACACAG CGTGGTGGTC
    AGCCAGCCAA ACAAAGAGAA CTCCTGCCAG GGCCATCTTT ACAACTCTCT GGGTCGGAAA
    GGAATCAGTG CTAAATCTCA GCCTTATAAC AGGTCCCAAT CATCTTCCTC CATCCTGATA
    AACAAGTCTG CAGACTCCAT CAACTACCCT AGTGGCGTGG CAAAGCAGCA GCCTGTCTCT
    CTACACAGAA GTTCAAGGTG GGAGAGTCAC CAGGACTTGC TGCCAAGTAT TGCTGACTCA
    GGTCAACAGG GCCCTGAAAA ACGCTCAGAT CTCACACTCC AGGACTCGCA GAAAGTTCTG
    GTAGTGAATA GAAATTTACC TTTAAGTGCT CAAATGGCTA CCCAGAACTA TTTTTCCAAT
    TTCAAAGAGA CTGAAGGAGA CGAAGATGAC TATGTGGAGA TCAAGTCAGA AGAAGATGAG
    TCTGAGTTAG AACTGTCTCA CAGTCGGAGG AGGAAATCTG ACCCAAAGAT TACAGACGCT
    GACTTTGCAG ATAACGTCTG CAGCCACACG ACTTCTTACT CTTTGAATAG TCCGTGCACT
    CCAAAAAAGC CAGTAAGTGG CAAACTTGGG ATTTCGCCGT ATCTGACGCC ATACAATGAT
    CCCGACAAAC TGAATGACTA TCTCTGGAAG GGCCCATCTC CCAATCAGCA AAATATTGTG
    CAGTCTCTAA GGGAAAAATT CCAGTGTCTT AGTTCAAGCA GCTTTGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852421.1
    CDS384..4694
    Translation

    Target ORF information:

    RefSeq Version XM_024996653.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology and RhoGEF domain containing G1 (PLEKHG1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996653.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGTGTCTGG GGAACCGATG CTCGACACCC TTGGTCCTTC CTGCTGGAGC AGCCGAGCCC 
    AACCGCCTCG ATGCGCACCA GTTGCGTCGT GAAGTACGGT CAGCTGTAAC ACAAGCTCGG
    TATCTCAGAA CCACAGCCAG GCACTCCTGC TCCGGGGACC TCGGGGCCAT GGAGCTCTCC
    GACAGTGACC GCCCGGTCAG CTTCGGCTCC ACGTCGTCCT CGGCTTCCTC CCGGGACAGC
    CATGGTTCCT TCGGCAGCAG AATGACCTTA GTTTCAAGCA GCCATCTGGG CTTGTTTCCT
    CAGGATAAGG AAGCAGGGGC CATAAAACTG GAGCTGATGC CAGCCAGGCC GTTTTCCAGC
    AGCGAGCTGC AGACGGACCG TGCGGCCGGG CAGCAGAGCA CAGACGAGGG CGGCGAGAGG
    CAGCCCCGGG CACAGCGCAG AGTGGAAGCC AACGGGCCGA CCAAAACCGG GGCCGAATCG
    GCCACCAGCC CCAAGCTGCT CTATGTGGAC AGAGTTGTGC AGGAAATTCT GGAGACCGAA
    CGGACTTACG TGCAAGATTT AAAGAGCATC GTAGAGGATT ACCTTGACTG TATCAGGGAC
    CAAACGAAAC TTCCTCTGGG GACAGATGAA AGATCAGCCC TTTTTGGAAA CATACAGGAT
    ATCTACCACT TTAATAGTGA ACTTCTGCAA GAGTTGGAAA ACTGTGAAAA TGATCCTGTG
    GCCATAGCAG AGTGCTTTGT GTCCAAGAGT GAAGAGTTCC ACATTTATAC CCAGTATTGC
    ACGAACTATC CAAGATCTGT GGCTGTGCTA ACAGAGTGTA TGAGGAACAA GATGCTGGCC
    AAATTCTTCA GGGAACGGCA GGAAACTTTG AAACATTCAT TGCCTCTGGG GTCCTATCTC
    TTGAAGCCAG TTCAGCGGAT TCTCAAGTAT CATCTCCTTT TGCATGAAAT AGAAAATCAC
    CTTGACAAGG ACACAGAGGG CTACGATGTG GTGCTTGATG CCATAGACAC GATGCAGAGA
    GTGGCCTGGC ACATCAATGA CATGAAGCGG AAGCATGAAC ACGCCGTCCG CCTGCAGGAG
    ATACAGAGTT TGCTCACCAA CTGGAAGGGG CCAGACCTGA CCAGCTACGG GGAGCTAGTG
    CTCGAAGGGA CCTTCCGCCT CCAGCGGGCC AAGAATGAGC GGACGCTCTT CCTCTTTGAC
    AAGCTGCTGC TCATCACCAA GAAGAGAGAC GACACGTTTA CATACAAAGC CCACATCCTG
    TGCGGCAACC TCATGCTGGT GGAAGTGATC CCAAAGGAGC CCCTCAGCTT CAGCGTCTTC
    CACTACAAGA ACCCCAAGCT GCAGCACACG GTTCAGGCGA AGTCGCAGCA AGACAAGCGC
    CTGTGGGTTC TGCACCTGAA GAGGCTGATC CTGGAGAATC ATGCGGCAAA GATTCCAGCT
    AAGGCCAAGC AAGCAATACT TGAAATGGAC GCCATTCATT ACCCAGGCTT CTGTTACAGT
    CCCGAGGGAG AGACTTGTGG CTCTCGAAAT GGCTCTGCTC CGTATCGGCT GAGAAGGAAA
    TCTGAACCTT CCTCAAGATC ACATAAAGTT TTGAAGACCA GTGAAACAGC ACAAGACATT
    CAAAAGGTTT CCAGAGAGGA AGGGTCTCCC CAGTTGACTT CAGCAGGGCC CTCTCCTGCC
    CAGCGGAACA GTCAGCCAAG CAGTTCGACC ATCCTGAGCG TGCTTCGTGG GGGTGGGGCT
    GTCAGAAACA TCTGGACCGA CCACCAGATC CGCCAAGCCC TGTTTCCCAG CCGAAGGTCG
    CAGGAGAACG AGGAGGATGA AGACGACTAT CAGATGTTCC TGCCATCCTT CTCCTCCTCG
    GATCTGAACT CAGCCCAGCT GTGTGAAGAT GGTACTTCTA GTCGACCTTG CAGTTGGCAC
    ATGGGACAAA TCGAGTCCTC CGAGACCTCC AGCTCTGGCC ACAGGATTGT GCGGCGGGCC
    AGCAGTGCTG GCGAGAGCAA CACGTGTCCC CCTGACATAA GAACTAGGGA CAGTGGTGGT
    TCTCAGTACA GCTCCCGGAG GGAACTGCAG GGTGACCCTA AAACAGAGGG GCAGGACGGG
    ACAACTCCCT ACGGGTCCTC TATAGAGTTG ACTATAGATG ACATAGACCA TGTCTATGAC
    AACATAAGTT ACGAGGACTT GAAACTGATG GTTGCTAAAC GCGAAGACGC TGAAGTCAGT
    CCCCAGAAAC CAGTCAGGGA CTCTGTTCGC CCCAAGAGCA CCCCAGAGCT GGCCTTCTCC
    AAGAAGCCAG CGGGGCCTGA GGAGGAGAGC ACATTGCACC CCAGGAGAGA TGGAGCCCTC
    CCGGCCGGGG AGGCCTGGAA CCAAAGCATG CACGACCTCC AGGTGATGGA GGAGAATGTC
    TATGACACCA TTGCGCTCCC GGAGGCGCCC TCCCGGGACT TCAAGGGCAG CAGCCCGAAG
    CGTTCTGCAA GGAGCACCTT CCTGGGCTTG GAGGCCGACT TGGGGTGCTG TGATAGTCTG
    CGGGCCTCTG CTTCCCAGGA CAGCCTCCAG TTCAGTGAGG ACGAGGCGCC CTACCCGCCA
    GGCTCCTCTG ATAACGATTA TCTGAGTTTG CTCTATAACT CCTTCGGCTG CAACTTGGCC
    CTAGCGGATC GGTCCATCTC TGATAAACTG TCTGAAGAAG TAGATGAAAT CTGGAATGAC
    TTGGAAAACT ACATCAAGAA AAACGAAGTC AAAGCCAGAA ACCGGCTCCT GGCCGCGTTT
    CCGGTTAGCA AAGATGACGT GCAGGAGCGG CCGCTGGCGG GCAGTACCCC GGAGCTGAGC
    GCGCGCTCCC CGCTCTCGCT GGCCGCGGGC CGGGCTCTCC TCGCGCCGCC GGCAGACAGG
    CCCGGGCCAG CCCCCTGCGC CCTGGACCGA GACCCAGCCT CCAAAGACAG CTCCTGCCTG
    AGTCTCCACC GGCTGTCGCT GGCCAGTGAC CTGCCGCCCG CAGAGAGTCC CTATGATGTG
    GCCGGCGGCA GCCTGTCCTC AGCGGACCTA GAGAACCCTG AGCCCGGGCT GGACAATGTA
    GACAAGACCA GGAGCAGGGT TTTCCTGATG GCCAGGCAGT ACAGTCAGAA GATCAAGAAG
    GCAAATCAGC TGCTAAAAGT GAAAAACCCA GAGCTGGAAC AGACGCTCAG TAGCCAGCCG
    CAGAAGCCCG CGTCCAAGGA CCTGGCGGCC ATCCTAGAAG AGAAGAGGCA GGGCGGGCCC
    GCCATCGGTG CCAGGATTGC TGAGTATTCC CAACTGTATG ACCAGATTGT ATTTAGAGAG
    ACCCCCTTTA AGACTCAGAA GGATGGCTGG GCCAGCCCTC AAGACCCCTC CAACCTCAGC
    TCTGCATCAC CTTCCCATGC CCAGCAAGGT AGCGAGGACT GGCTTTTGCA TTCAACCTAT
    AGTAATGGAG AGTTAGCTGA CTTCTGTCCC TGGCCAGAAC AAGACTTAAA GTCAAAATAT
    CCCATCTTAG AGATCAATGC AAGAAGTACC CCAAAACAAC TGTCTGCAGC TTGCTCTGTG
    CCTTCTCTTC AAACCTCCGA CCCTCTGCTG GGCTTCGTGC AGAGACACAG CGTGGTGGTC
    AGCCAGCCAA ACAAAGAGAA CTCCTGCCAG GGCCATCTTT ACAACTCTCT GGGTCGGAAA
    GGAATCAGTG CTAAATCTCA GCCTTATAAC AGGTCCCAAT CATCTTCCTC CATCCTGATA
    AACAAGTCTG CAGACTCCAT CAACTACCCT AGTGGCGTGG CAAAGCAGCA GCCTGTCTCT
    CTACACAGAA GTTCAAGGTG GGAGAGTCAC CAGGACTTGC TGCCAAGTAT TGCTGACTCA
    GGTCAACAGG GCCCTGAAAA ACGCTCAGAT CTCACACTCC AGGACTCGCA GAAAGTTCTG
    GTAGTGAATA GAAATTTACC TTTAAGTGCT CAAATGGCTA CCCAGAACTA TTTTTCCAAT
    TTCAAAGAGA CTGAAGGAGA CGAAGATGAC TATGTGGAGA TCAAGTCAGA AGAAGATGAG
    TCTGAGTTAG AACTGTCTCA CAGTCGGAGG AGGAAATCTG ACCCAAAGAT TACAGACGCT
    GACTTTGCAG ATAACGTCTG CAGCCACACG ACTTCTTACT CTTTGAATAG TCCGTGCACT
    CCAAAAAAGC CAGTAAGTGG CAAACTTGGG ATTTCGCCGT ATCTGACGCC ATACAATGAT
    CCCGACAAAC TGAATGACTA TCTCTGGAAG GGCCCATCTC CCAATCAGCA AAATATTGTG
    CAGTCTCTAA GGGAAAAATT CCAGTGTCTT AGTTCAAGCA GCTTTGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237778
    Clone ID Related Accession (Same CDS sequence) XM_024996655.1 , XM_024996658.1 , XM_024996654.1 , NM_001205921.2 , XM_024996656.1
    Accession Version XM_024996655.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4143bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pleckstrin homology domain-containing family G member 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAGCTCT CCGACAGTGA CCGCCCGGTC AGCTTCGGCT CCACGTCGTC CTCGGCTTCC 
    TCCCGGGACA GCCATGGTTC CTTCGGCAGC AGAATGACCT TAGTTTCAAG CAGCCATCTG
    GGCTTGTTTC CTCAGGATAA GGAAGCAGGG GCCATAAAAC TGGAGCTGAT GCCAGCCAGG
    CCGTTTTCCA GCAGCGAGCT GCAGACGGAC CGTGCGGCCG GGCAGCAGAG CACAGACGAG
    GGCGGCGAGA GGCAGCCCCG GGCACAGCGC AGAGTGGAAG CCAACGGGCC GACCAAAACC
    GGGGCCGAAT CGGCCACCAG CCCCAAGCTG CTCTATGTGG ACAGAGTTGT GCAGGAAATT
    CTGGAGACCG AACGGACTTA CGTGCAAGAT TTAAAGAGCA TCGTAGAGGA TTACCTTGAC
    TGTATCAGGG ACCAAACGAA ACTTCCTCTG GGGACAGATG AAAGATCAGC CCTTTTTGGA
    AACATACAGG ATATCTACCA CTTTAATAGT GAACTTCTGC AAGAGTTGGA AAACTGTGAA
    AATGATCCTG TGGCCATAGC AGAGTGCTTT GTGTCCAAGA GTGAAGAGTT CCACATTTAT
    ACCCAGTATT GCACGAACTA TCCAAGATCT GTGGCTGTGC TAACAGAGTG TATGAGGAAC
    AAGATGCTGG CCAAATTCTT CAGGGAACGG CAGGAAACTT TGAAACATTC ATTGCCTCTG
    GGGTCCTATC TCTTGAAGCC AGTTCAGCGG ATTCTCAAGT ATCATCTCCT TTTGCATGAA
    ATAGAAAATC ACCTTGACAA GGACACAGAG GGCTACGATG TGGTGCTTGA TGCCATAGAC
    ACGATGCAGA GAGTGGCCTG GCACATCAAT GACATGAAGC GGAAGCATGA ACACGCCGTC
    CGCCTGCAGG AGATACAGAG TTTGCTCACC AACTGGAAGG GGCCAGACCT GACCAGCTAC
    GGGGAGCTAG TGCTCGAAGG GACCTTCCGC CTCCAGCGGG CCAAGAATGA GCGGACGCTC
    TTCCTCTTTG ACAAGCTGCT GCTCATCACC AAGAAGAGAG ACGACACGTT TACATACAAA
    GCCCACATCC TGTGCGGCAA CCTCATGCTG GTGGAAGTGA TCCCAAAGGA GCCCCTCAGC
    TTCAGCGTCT TCCACTACAA GAACCCCAAG CTGCAGCACA CGGTTCAGGC GAAGTCGCAG
    CAAGACAAGC GCCTGTGGGT TCTGCACCTG AAGAGGCTGA TCCTGGAGAA TCATGCGGCA
    AAGATTCCAG CTAAGGCCAA GCAAGCAATA CTTGAAATGG ACGCCATTCA TTACCCAGGC
    TTCTGTTACA GTCCCGAGGG AGAGACTTGT GGCTCTCGAA ATGGCTCTGC TCCGTATCGG
    CTGAGAAGGA AATCTGAACC TTCCTCAAGA TCACATAAAG TTTTGAAGAC CAGTGAAACA
    GCACAAGACA TTCAAAAGGT TTCCAGAGAG GAAGGGTCTC CCCAGTTGAC TTCAGCAGGG
    CCCTCTCCTG CCCAGCGGAA CAGTCAGCCA AGCAGTTCGA CCATCCTGAG CGTGCTTCGT
    GGGGGTGGGG CTGTCAGAAA CATCTGGACC GACCACCAGA TCCGCCAAGC CCTGTTTCCC
    AGCCGAAGGT CGCAGGAGAA CGAGGAGGAT GAAGACGACT ATCAGATGTT CCTGCCATCC
    TTCTCCTCCT CGGATCTGAA CTCAGCCCAG CTGTGTGAAG ATGGTACTTC TAGTCGACCT
    TGCAGTTGGC ACATGGGACA AATCGAGTCC TCCGAGACCT CCAGCTCTGG CCACAGGATT
    GTGCGGCGGG CCAGCAGTGC TGGCGAGAGC AACACGTGTC CCCCTGACAT AAGAACTAGG
    GACAGTGGTG GTTCTCAGTA CAGCTCCCGG AGGGAACTGC AGGGTGACCC TAAAACAGAG
    GGGCAGGACG GGACAACTCC CTACGGGTCC TCTATAGAGT TGACTATAGA TGACATAGAC
    CATGTCTATG ACAACATAAG TTACGAGGAC TTGAAACTGA TGGTTGCTAA ACGCGAAGAC
    GCTGAAGTCA GTCCCCAGAA ACCAGTCAGG GACTCTGTTC GCCCCAAGAG CACCCCAGAG
    CTGGCCTTCT CCAAGAAGCC AGCGGGGCCT GAGGAGGAGA GCACATTGCA CCCCAGGAGA
    GATGGAGCCC TCCCGGCCGG GGAGGCCTGG AACCAAAGCA TGCACGACCT CCAGGTGATG
    GAGGAGAATG TCTATGACAC CATTGCGCTC CCGGAGGCGC CCTCCCGGGA CTTCAAGGGC
    AGCAGCCCGA AGCGTTCTGC AAGGAGCACC TTCCTGGGCT TGGAGGCCGA CTTGGGGTGC
    TGTGATAGTC TGCGGGCCTC TGCTTCCCAG GACAGCCTCC AGTTCAGTGA GGACGAGGCG
    CCCTACCCGC CAGGCTCCTC TGATAACGAT TATCTGAGTT TGCTCTATAA CTCCTTCGGC
    TGCAACTTGG CCCTAGCGGA TCGGTCCATC TCTGATAAAC TGTCTGAAGA AGTAGATGAA
    ATCTGGAATG ACTTGGAAAA CTACATCAAG AAAAACGAAG TCAAAGCCAG AAACCGGCTC
    CTGGCCGCGT TTCCGGTTAG CAAAGATGAC GTGCAGGAGC GGCCGCTGGC GGGCAGTACC
    CCGGAGCTGA GCGCGCGCTC CCCGCTCTCG CTGGCCGCGG GCCGGGCTCT CCTCGCGCCG
    CCGGCAGACA GGCCCGGGCC AGCCCCCTGC GCCCTGGACC GAGACCCAGC CTCCAAAGAC
    AGCTCCTGCC TGAGTCTCCA CCGGCTGTCG CTGGCCAGTG ACCTGCCGCC CGCAGAGAGT
    CCCTATGATG TGGCCGGCGG CAGCCTGTCC TCAGCGGACC TAGAGAACCC TGAGCCCGGG
    CTGGACAATG TAGACAAGAC CAGGAGCAGG GTTTTCCTGA TGGCCAGGCA GTACAGTCAG
    AAGATCAAGA AGGCAAATCA GCTGCTAAAA GTGAAAAACC CAGAGCTGGA ACAGACGCTC
    AGTAGCCAGC CGCAGAAGCC CGCGTCCAAG GACCTGGCGG CCATCCTAGA AGAGAAGAGG
    CAGGGCGGGC CCGCCATCGG TGCCAGGATT GCTGAGTATT CCCAACTGTA TGACCAGATT
    GTATTTAGAG AGACCCCCTT TAAGACTCAG AAGGATGGCT GGGCCAGCCC TCAAGACCCC
    TCCAACCTCA GCTCTGCATC ACCTTCCCAT GCCCAGCAAG GTAGCGAGGA CTGGCTTTTG
    CATTCAACCT ATAGTAATGG AGAGTTAGCT GACTTCTGTC CCTGGCCAGA ACAAGACTTA
    AAGTCAAAAT ATCCCATCTT AGAGATCAAT GCAAGAAGTA CCCCAAAACA ACTGTCTGCA
    GCTTGCTCTG TGCCTTCTCT TCAAACCTCC GACCCTCTGC TGGGCTTCGT GCAGAGACAC
    AGCGTGGTGG TCAGCCAGCC AAACAAAGAG AACTCCTGCC AGGGCCATCT TTACAACTCT
    CTGGGTCGGA AAGGAATCAG TGCTAAATCT CAGCCTTATA ACAGGTCCCA ATCATCTTCC
    TCCATCCTGA TAAACAAGTC TGCAGACTCC ATCAACTACC CTAGTGGCGT GGCAAAGCAG
    CAGCCTGTCT CTCTACACAG AAGTTCAAGG TGGGAGAGTC ACCAGGACTT GCTGCCAAGT
    ATTGCTGACT CAGGTCAACA GGGCCCTGAA AAACGCTCAG ATCTCACACT CCAGGACTCG
    CAGAAAGTTC TGGTAGTGAA TAGAAATTTA CCTTTAAGTG CTCAAATGGC TACCCAGAAC
    TATTTTTCCA ATTTCAAAGA GACTGAAGGA GACGAAGATG ACTATGTGGA GATCAAGTCA
    GAAGAAGATG AGTCTGAGTT AGAACTGTCT CACAGTCGGA GGAGGAAATC TGACCCAAAG
    ATTACAGACG CTGACTTTGC AGATAACGTC TGCAGCCACA CGACTTCTTA CTCTTTGAAT
    AGTCCGTGCA CTCCAAAAAA GCCAGTAAGT GGCAAACTTG GGATTTCGCC GTATCTGACG
    CCATACAATG ATCCCGACAA ACTGAATGAC TATCTCTGGA AGGGCCCATC TCCCAATCAG
    CAAAATATTG TGCAGTCTCT AAGGGAAAAA TTCCAGTGTC TTAGTTCAAG CAGCTTTGCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852423.1
    CDS599..4741
    Translation

    Target ORF information:

    RefSeq Version XM_024996655.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology and RhoGEF domain containing G1 (PLEKHG1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996655.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAGCTCT CCGACAGTGA CCGCCCGGTC AGCTTCGGCT CCACGTCGTC CTCGGCTTCC 
    TCCCGGGACA GCCATGGTTC CTTCGGCAGC AGAATGACCT TAGTTTCAAG CAGCCATCTG
    GGCTTGTTTC CTCAGGATAA GGAAGCAGGG GCCATAAAAC TGGAGCTGAT GCCAGCCAGG
    CCGTTTTCCA GCAGCGAGCT GCAGACGGAC CGTGCGGCCG GGCAGCAGAG CACAGACGAG
    GGCGGCGAGA GGCAGCCCCG GGCACAGCGC AGAGTGGAAG CCAACGGGCC GACCAAAACC
    GGGGCCGAAT CGGCCACCAG CCCCAAGCTG CTCTATGTGG ACAGAGTTGT GCAGGAAATT
    CTGGAGACCG AACGGACTTA CGTGCAAGAT TTAAAGAGCA TCGTAGAGGA TTACCTTGAC
    TGTATCAGGG ACCAAACGAA ACTTCCTCTG GGGACAGATG AAAGATCAGC CCTTTTTGGA
    AACATACAGG ATATCTACCA CTTTAATAGT GAACTTCTGC AAGAGTTGGA AAACTGTGAA
    AATGATCCTG TGGCCATAGC AGAGTGCTTT GTGTCCAAGA GTGAAGAGTT CCACATTTAT
    ACCCAGTATT GCACGAACTA TCCAAGATCT GTGGCTGTGC TAACAGAGTG TATGAGGAAC
    AAGATGCTGG CCAAATTCTT CAGGGAACGG CAGGAAACTT TGAAACATTC ATTGCCTCTG
    GGGTCCTATC TCTTGAAGCC AGTTCAGCGG ATTCTCAAGT ATCATCTCCT TTTGCATGAA
    ATAGAAAATC ACCTTGACAA GGACACAGAG GGCTACGATG TGGTGCTTGA TGCCATAGAC
    ACGATGCAGA GAGTGGCCTG GCACATCAAT GACATGAAGC GGAAGCATGA ACACGCCGTC
    CGCCTGCAGG AGATACAGAG TTTGCTCACC AACTGGAAGG GGCCAGACCT GACCAGCTAC
    GGGGAGCTAG TGCTCGAAGG GACCTTCCGC CTCCAGCGGG CCAAGAATGA GCGGACGCTC
    TTCCTCTTTG ACAAGCTGCT GCTCATCACC AAGAAGAGAG ACGACACGTT TACATACAAA
    GCCCACATCC TGTGCGGCAA CCTCATGCTG GTGGAAGTGA TCCCAAAGGA GCCCCTCAGC
    TTCAGCGTCT TCCACTACAA GAACCCCAAG CTGCAGCACA CGGTTCAGGC GAAGTCGCAG
    CAAGACAAGC GCCTGTGGGT TCTGCACCTG AAGAGGCTGA TCCTGGAGAA TCATGCGGCA
    AAGATTCCAG CTAAGGCCAA GCAAGCAATA CTTGAAATGG ACGCCATTCA TTACCCAGGC
    TTCTGTTACA GTCCCGAGGG AGAGACTTGT GGCTCTCGAA ATGGCTCTGC TCCGTATCGG
    CTGAGAAGGA AATCTGAACC TTCCTCAAGA TCACATAAAG TTTTGAAGAC CAGTGAAACA
    GCACAAGACA TTCAAAAGGT TTCCAGAGAG GAAGGGTCTC CCCAGTTGAC TTCAGCAGGG
    CCCTCTCCTG CCCAGCGGAA CAGTCAGCCA AGCAGTTCGA CCATCCTGAG CGTGCTTCGT
    GGGGGTGGGG CTGTCAGAAA CATCTGGACC GACCACCAGA TCCGCCAAGC CCTGTTTCCC
    AGCCGAAGGT CGCAGGAGAA CGAGGAGGAT GAAGACGACT ATCAGATGTT CCTGCCATCC
    TTCTCCTCCT CGGATCTGAA CTCAGCCCAG CTGTGTGAAG ATGGTACTTC TAGTCGACCT
    TGCAGTTGGC ACATGGGACA AATCGAGTCC TCCGAGACCT CCAGCTCTGG CCACAGGATT
    GTGCGGCGGG CCAGCAGTGC TGGCGAGAGC AACACGTGTC CCCCTGACAT AAGAACTAGG
    GACAGTGGTG GTTCTCAGTA CAGCTCCCGG AGGGAACTGC AGGGTGACCC TAAAACAGAG
    GGGCAGGACG GGACAACTCC CTACGGGTCC TCTATAGAGT TGACTATAGA TGACATAGAC
    CATGTCTATG ACAACATAAG TTACGAGGAC TTGAAACTGA TGGTTGCTAA ACGCGAAGAC
    GCTGAAGTCA GTCCCCAGAA ACCAGTCAGG GACTCTGTTC GCCCCAAGAG CACCCCAGAG
    CTGGCCTTCT CCAAGAAGCC AGCGGGGCCT GAGGAGGAGA GCACATTGCA CCCCAGGAGA
    GATGGAGCCC TCCCGGCCGG GGAGGCCTGG AACCAAAGCA TGCACGACCT CCAGGTGATG
    GAGGAGAATG TCTATGACAC CATTGCGCTC CCGGAGGCGC CCTCCCGGGA CTTCAAGGGC
    AGCAGCCCGA AGCGTTCTGC AAGGAGCACC TTCCTGGGCT TGGAGGCCGA CTTGGGGTGC
    TGTGATAGTC TGCGGGCCTC TGCTTCCCAG GACAGCCTCC AGTTCAGTGA GGACGAGGCG
    CCCTACCCGC CAGGCTCCTC TGATAACGAT TATCTGAGTT TGCTCTATAA CTCCTTCGGC
    TGCAACTTGG CCCTAGCGGA TCGGTCCATC TCTGATAAAC TGTCTGAAGA AGTAGATGAA
    ATCTGGAATG ACTTGGAAAA CTACATCAAG AAAAACGAAG TCAAAGCCAG AAACCGGCTC
    CTGGCCGCGT TTCCGGTTAG CAAAGATGAC GTGCAGGAGC GGCCGCTGGC GGGCAGTACC
    CCGGAGCTGA GCGCGCGCTC CCCGCTCTCG CTGGCCGCGG GCCGGGCTCT CCTCGCGCCG
    CCGGCAGACA GGCCCGGGCC AGCCCCCTGC GCCCTGGACC GAGACCCAGC CTCCAAAGAC
    AGCTCCTGCC TGAGTCTCCA CCGGCTGTCG CTGGCCAGTG ACCTGCCGCC CGCAGAGAGT
    CCCTATGATG TGGCCGGCGG CAGCCTGTCC TCAGCGGACC TAGAGAACCC TGAGCCCGGG
    CTGGACAATG TAGACAAGAC CAGGAGCAGG GTTTTCCTGA TGGCCAGGCA GTACAGTCAG
    AAGATCAAGA AGGCAAATCA GCTGCTAAAA GTGAAAAACC CAGAGCTGGA ACAGACGCTC
    AGTAGCCAGC CGCAGAAGCC CGCGTCCAAG GACCTGGCGG CCATCCTAGA AGAGAAGAGG
    CAGGGCGGGC CCGCCATCGG TGCCAGGATT GCTGAGTATT CCCAACTGTA TGACCAGATT
    GTATTTAGAG AGACCCCCTT TAAGACTCAG AAGGATGGCT GGGCCAGCCC TCAAGACCCC
    TCCAACCTCA GCTCTGCATC ACCTTCCCAT GCCCAGCAAG GTAGCGAGGA CTGGCTTTTG
    CATTCAACCT ATAGTAATGG AGAGTTAGCT GACTTCTGTC CCTGGCCAGA ACAAGACTTA
    AAGTCAAAAT ATCCCATCTT AGAGATCAAT GCAAGAAGTA CCCCAAAACA ACTGTCTGCA
    GCTTGCTCTG TGCCTTCTCT TCAAACCTCC GACCCTCTGC TGGGCTTCGT GCAGAGACAC
    AGCGTGGTGG TCAGCCAGCC AAACAAAGAG AACTCCTGCC AGGGCCATCT TTACAACTCT
    CTGGGTCGGA AAGGAATCAG TGCTAAATCT CAGCCTTATA ACAGGTCCCA ATCATCTTCC
    TCCATCCTGA TAAACAAGTC TGCAGACTCC ATCAACTACC CTAGTGGCGT GGCAAAGCAG
    CAGCCTGTCT CTCTACACAG AAGTTCAAGG TGGGAGAGTC ACCAGGACTT GCTGCCAAGT
    ATTGCTGACT CAGGTCAACA GGGCCCTGAA AAACGCTCAG ATCTCACACT CCAGGACTCG
    CAGAAAGTTC TGGTAGTGAA TAGAAATTTA CCTTTAAGTG CTCAAATGGC TACCCAGAAC
    TATTTTTCCA ATTTCAAAGA GACTGAAGGA GACGAAGATG ACTATGTGGA GATCAAGTCA
    GAAGAAGATG AGTCTGAGTT AGAACTGTCT CACAGTCGGA GGAGGAAATC TGACCCAAAG
    ATTACAGACG CTGACTTTGC AGATAACGTC TGCAGCCACA CGACTTCTTA CTCTTTGAAT
    AGTCCGTGCA CTCCAAAAAA GCCAGTAAGT GGCAAACTTG GGATTTCGCC GTATCTGACG
    CCATACAATG ATCCCGACAA ACTGAATGAC TATCTCTGGA AGGGCCCATC TCCCAATCAG
    CAAAATATTG TGCAGTCTCT AAGGGAAAAA TTCCAGTGTC TTAGTTCAAG CAGCTTTGCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237778
    Clone ID Related Accession (Same CDS sequence) XM_024996655.1 , XM_024996658.1 , XM_024996654.1 , NM_001205921.2 , XM_024996656.1
    Accession Version XM_024996658.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4143bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pleckstrin homology domain-containing family G member 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAGCTCT CCGACAGTGA CCGCCCGGTC AGCTTCGGCT CCACGTCGTC CTCGGCTTCC 
    TCCCGGGACA GCCATGGTTC CTTCGGCAGC AGAATGACCT TAGTTTCAAG CAGCCATCTG
    GGCTTGTTTC CTCAGGATAA GGAAGCAGGG GCCATAAAAC TGGAGCTGAT GCCAGCCAGG
    CCGTTTTCCA GCAGCGAGCT GCAGACGGAC CGTGCGGCCG GGCAGCAGAG CACAGACGAG
    GGCGGCGAGA GGCAGCCCCG GGCACAGCGC AGAGTGGAAG CCAACGGGCC GACCAAAACC
    GGGGCCGAAT CGGCCACCAG CCCCAAGCTG CTCTATGTGG ACAGAGTTGT GCAGGAAATT
    CTGGAGACCG AACGGACTTA CGTGCAAGAT TTAAAGAGCA TCGTAGAGGA TTACCTTGAC
    TGTATCAGGG ACCAAACGAA ACTTCCTCTG GGGACAGATG AAAGATCAGC CCTTTTTGGA
    AACATACAGG ATATCTACCA CTTTAATAGT GAACTTCTGC AAGAGTTGGA AAACTGTGAA
    AATGATCCTG TGGCCATAGC AGAGTGCTTT GTGTCCAAGA GTGAAGAGTT CCACATTTAT
    ACCCAGTATT GCACGAACTA TCCAAGATCT GTGGCTGTGC TAACAGAGTG TATGAGGAAC
    AAGATGCTGG CCAAATTCTT CAGGGAACGG CAGGAAACTT TGAAACATTC ATTGCCTCTG
    GGGTCCTATC TCTTGAAGCC AGTTCAGCGG ATTCTCAAGT ATCATCTCCT TTTGCATGAA
    ATAGAAAATC ACCTTGACAA GGACACAGAG GGCTACGATG TGGTGCTTGA TGCCATAGAC
    ACGATGCAGA GAGTGGCCTG GCACATCAAT GACATGAAGC GGAAGCATGA ACACGCCGTC
    CGCCTGCAGG AGATACAGAG TTTGCTCACC AACTGGAAGG GGCCAGACCT GACCAGCTAC
    GGGGAGCTAG TGCTCGAAGG GACCTTCCGC CTCCAGCGGG CCAAGAATGA GCGGACGCTC
    TTCCTCTTTG ACAAGCTGCT GCTCATCACC AAGAAGAGAG ACGACACGTT TACATACAAA
    GCCCACATCC TGTGCGGCAA CCTCATGCTG GTGGAAGTGA TCCCAAAGGA GCCCCTCAGC
    TTCAGCGTCT TCCACTACAA GAACCCCAAG CTGCAGCACA CGGTTCAGGC GAAGTCGCAG
    CAAGACAAGC GCCTGTGGGT TCTGCACCTG AAGAGGCTGA TCCTGGAGAA TCATGCGGCA
    AAGATTCCAG CTAAGGCCAA GCAAGCAATA CTTGAAATGG ACGCCATTCA TTACCCAGGC
    TTCTGTTACA GTCCCGAGGG AGAGACTTGT GGCTCTCGAA ATGGCTCTGC TCCGTATCGG
    CTGAGAAGGA AATCTGAACC TTCCTCAAGA TCACATAAAG TTTTGAAGAC CAGTGAAACA
    GCACAAGACA TTCAAAAGGT TTCCAGAGAG GAAGGGTCTC CCCAGTTGAC TTCAGCAGGG
    CCCTCTCCTG CCCAGCGGAA CAGTCAGCCA AGCAGTTCGA CCATCCTGAG CGTGCTTCGT
    GGGGGTGGGG CTGTCAGAAA CATCTGGACC GACCACCAGA TCCGCCAAGC CCTGTTTCCC
    AGCCGAAGGT CGCAGGAGAA CGAGGAGGAT GAAGACGACT ATCAGATGTT CCTGCCATCC
    TTCTCCTCCT CGGATCTGAA CTCAGCCCAG CTGTGTGAAG ATGGTACTTC TAGTCGACCT
    TGCAGTTGGC ACATGGGACA AATCGAGTCC TCCGAGACCT CCAGCTCTGG CCACAGGATT
    GTGCGGCGGG CCAGCAGTGC TGGCGAGAGC AACACGTGTC CCCCTGACAT AAGAACTAGG
    GACAGTGGTG GTTCTCAGTA CAGCTCCCGG AGGGAACTGC AGGGTGACCC TAAAACAGAG
    GGGCAGGACG GGACAACTCC CTACGGGTCC TCTATAGAGT TGACTATAGA TGACATAGAC
    CATGTCTATG ACAACATAAG TTACGAGGAC TTGAAACTGA TGGTTGCTAA ACGCGAAGAC
    GCTGAAGTCA GTCCCCAGAA ACCAGTCAGG GACTCTGTTC GCCCCAAGAG CACCCCAGAG
    CTGGCCTTCT CCAAGAAGCC AGCGGGGCCT GAGGAGGAGA GCACATTGCA CCCCAGGAGA
    GATGGAGCCC TCCCGGCCGG GGAGGCCTGG AACCAAAGCA TGCACGACCT CCAGGTGATG
    GAGGAGAATG TCTATGACAC CATTGCGCTC CCGGAGGCGC CCTCCCGGGA CTTCAAGGGC
    AGCAGCCCGA AGCGTTCTGC AAGGAGCACC TTCCTGGGCT TGGAGGCCGA CTTGGGGTGC
    TGTGATAGTC TGCGGGCCTC TGCTTCCCAG GACAGCCTCC AGTTCAGTGA GGACGAGGCG
    CCCTACCCGC CAGGCTCCTC TGATAACGAT TATCTGAGTT TGCTCTATAA CTCCTTCGGC
    TGCAACTTGG CCCTAGCGGA TCGGTCCATC TCTGATAAAC TGTCTGAAGA AGTAGATGAA
    ATCTGGAATG ACTTGGAAAA CTACATCAAG AAAAACGAAG TCAAAGCCAG AAACCGGCTC
    CTGGCCGCGT TTCCGGTTAG CAAAGATGAC GTGCAGGAGC GGCCGCTGGC GGGCAGTACC
    CCGGAGCTGA GCGCGCGCTC CCCGCTCTCG CTGGCCGCGG GCCGGGCTCT CCTCGCGCCG
    CCGGCAGACA GGCCCGGGCC AGCCCCCTGC GCCCTGGACC GAGACCCAGC CTCCAAAGAC
    AGCTCCTGCC TGAGTCTCCA CCGGCTGTCG CTGGCCAGTG ACCTGCCGCC CGCAGAGAGT
    CCCTATGATG TGGCCGGCGG CAGCCTGTCC TCAGCGGACC TAGAGAACCC TGAGCCCGGG
    CTGGACAATG TAGACAAGAC CAGGAGCAGG GTTTTCCTGA TGGCCAGGCA GTACAGTCAG
    AAGATCAAGA AGGCAAATCA GCTGCTAAAA GTGAAAAACC CAGAGCTGGA ACAGACGCTC
    AGTAGCCAGC CGCAGAAGCC CGCGTCCAAG GACCTGGCGG CCATCCTAGA AGAGAAGAGG
    CAGGGCGGGC CCGCCATCGG TGCCAGGATT GCTGAGTATT CCCAACTGTA TGACCAGATT
    GTATTTAGAG AGACCCCCTT TAAGACTCAG AAGGATGGCT GGGCCAGCCC TCAAGACCCC
    TCCAACCTCA GCTCTGCATC ACCTTCCCAT GCCCAGCAAG GTAGCGAGGA CTGGCTTTTG
    CATTCAACCT ATAGTAATGG AGAGTTAGCT GACTTCTGTC CCTGGCCAGA ACAAGACTTA
    AAGTCAAAAT ATCCCATCTT AGAGATCAAT GCAAGAAGTA CCCCAAAACA ACTGTCTGCA
    GCTTGCTCTG TGCCTTCTCT TCAAACCTCC GACCCTCTGC TGGGCTTCGT GCAGAGACAC
    AGCGTGGTGG TCAGCCAGCC AAACAAAGAG AACTCCTGCC AGGGCCATCT TTACAACTCT
    CTGGGTCGGA AAGGAATCAG TGCTAAATCT CAGCCTTATA ACAGGTCCCA ATCATCTTCC
    TCCATCCTGA TAAACAAGTC TGCAGACTCC ATCAACTACC CTAGTGGCGT GGCAAAGCAG
    CAGCCTGTCT CTCTACACAG AAGTTCAAGG TGGGAGAGTC ACCAGGACTT GCTGCCAAGT
    ATTGCTGACT CAGGTCAACA GGGCCCTGAA AAACGCTCAG ATCTCACACT CCAGGACTCG
    CAGAAAGTTC TGGTAGTGAA TAGAAATTTA CCTTTAAGTG CTCAAATGGC TACCCAGAAC
    TATTTTTCCA ATTTCAAAGA GACTGAAGGA GACGAAGATG ACTATGTGGA GATCAAGTCA
    GAAGAAGATG AGTCTGAGTT AGAACTGTCT CACAGTCGGA GGAGGAAATC TGACCCAAAG
    ATTACAGACG CTGACTTTGC AGATAACGTC TGCAGCCACA CGACTTCTTA CTCTTTGAAT
    AGTCCGTGCA CTCCAAAAAA GCCAGTAAGT GGCAAACTTG GGATTTCGCC GTATCTGACG
    CCATACAATG ATCCCGACAA ACTGAATGAC TATCTCTGGA AGGGCCCATC TCCCAATCAG
    CAAAATATTG TGCAGTCTCT AAGGGAAAAA TTCCAGTGTC TTAGTTCAAG CAGCTTTGCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852426.1
    CDS213..4355
    Translation

    Target ORF information:

    RefSeq Version XM_024996658.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology and RhoGEF domain containing G1 (PLEKHG1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996658.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAGCTCT CCGACAGTGA CCGCCCGGTC AGCTTCGGCT CCACGTCGTC CTCGGCTTCC 
    TCCCGGGACA GCCATGGTTC CTTCGGCAGC AGAATGACCT TAGTTTCAAG CAGCCATCTG
    GGCTTGTTTC CTCAGGATAA GGAAGCAGGG GCCATAAAAC TGGAGCTGAT GCCAGCCAGG
    CCGTTTTCCA GCAGCGAGCT GCAGACGGAC CGTGCGGCCG GGCAGCAGAG CACAGACGAG
    GGCGGCGAGA GGCAGCCCCG GGCACAGCGC AGAGTGGAAG CCAACGGGCC GACCAAAACC
    GGGGCCGAAT CGGCCACCAG CCCCAAGCTG CTCTATGTGG ACAGAGTTGT GCAGGAAATT
    CTGGAGACCG AACGGACTTA CGTGCAAGAT TTAAAGAGCA TCGTAGAGGA TTACCTTGAC
    TGTATCAGGG ACCAAACGAA ACTTCCTCTG GGGACAGATG AAAGATCAGC CCTTTTTGGA
    AACATACAGG ATATCTACCA CTTTAATAGT GAACTTCTGC AAGAGTTGGA AAACTGTGAA
    AATGATCCTG TGGCCATAGC AGAGTGCTTT GTGTCCAAGA GTGAAGAGTT CCACATTTAT
    ACCCAGTATT GCACGAACTA TCCAAGATCT GTGGCTGTGC TAACAGAGTG TATGAGGAAC
    AAGATGCTGG CCAAATTCTT CAGGGAACGG CAGGAAACTT TGAAACATTC ATTGCCTCTG
    GGGTCCTATC TCTTGAAGCC AGTTCAGCGG ATTCTCAAGT ATCATCTCCT TTTGCATGAA
    ATAGAAAATC ACCTTGACAA GGACACAGAG GGCTACGATG TGGTGCTTGA TGCCATAGAC
    ACGATGCAGA GAGTGGCCTG GCACATCAAT GACATGAAGC GGAAGCATGA ACACGCCGTC
    CGCCTGCAGG AGATACAGAG TTTGCTCACC AACTGGAAGG GGCCAGACCT GACCAGCTAC
    GGGGAGCTAG TGCTCGAAGG GACCTTCCGC CTCCAGCGGG CCAAGAATGA GCGGACGCTC
    TTCCTCTTTG ACAAGCTGCT GCTCATCACC AAGAAGAGAG ACGACACGTT TACATACAAA
    GCCCACATCC TGTGCGGCAA CCTCATGCTG GTGGAAGTGA TCCCAAAGGA GCCCCTCAGC
    TTCAGCGTCT TCCACTACAA GAACCCCAAG CTGCAGCACA CGGTTCAGGC GAAGTCGCAG
    CAAGACAAGC GCCTGTGGGT TCTGCACCTG AAGAGGCTGA TCCTGGAGAA TCATGCGGCA
    AAGATTCCAG CTAAGGCCAA GCAAGCAATA CTTGAAATGG ACGCCATTCA TTACCCAGGC
    TTCTGTTACA GTCCCGAGGG AGAGACTTGT GGCTCTCGAA ATGGCTCTGC TCCGTATCGG
    CTGAGAAGGA AATCTGAACC TTCCTCAAGA TCACATAAAG TTTTGAAGAC CAGTGAAACA
    GCACAAGACA TTCAAAAGGT TTCCAGAGAG GAAGGGTCTC CCCAGTTGAC TTCAGCAGGG
    CCCTCTCCTG CCCAGCGGAA CAGTCAGCCA AGCAGTTCGA CCATCCTGAG CGTGCTTCGT
    GGGGGTGGGG CTGTCAGAAA CATCTGGACC GACCACCAGA TCCGCCAAGC CCTGTTTCCC
    AGCCGAAGGT CGCAGGAGAA CGAGGAGGAT GAAGACGACT ATCAGATGTT CCTGCCATCC
    TTCTCCTCCT CGGATCTGAA CTCAGCCCAG CTGTGTGAAG ATGGTACTTC TAGTCGACCT
    TGCAGTTGGC ACATGGGACA AATCGAGTCC TCCGAGACCT CCAGCTCTGG CCACAGGATT
    GTGCGGCGGG CCAGCAGTGC TGGCGAGAGC AACACGTGTC CCCCTGACAT AAGAACTAGG
    GACAGTGGTG GTTCTCAGTA CAGCTCCCGG AGGGAACTGC AGGGTGACCC TAAAACAGAG
    GGGCAGGACG GGACAACTCC CTACGGGTCC TCTATAGAGT TGACTATAGA TGACATAGAC
    CATGTCTATG ACAACATAAG TTACGAGGAC TTGAAACTGA TGGTTGCTAA ACGCGAAGAC
    GCTGAAGTCA GTCCCCAGAA ACCAGTCAGG GACTCTGTTC GCCCCAAGAG CACCCCAGAG
    CTGGCCTTCT CCAAGAAGCC AGCGGGGCCT GAGGAGGAGA GCACATTGCA CCCCAGGAGA
    GATGGAGCCC TCCCGGCCGG GGAGGCCTGG AACCAAAGCA TGCACGACCT CCAGGTGATG
    GAGGAGAATG TCTATGACAC CATTGCGCTC CCGGAGGCGC CCTCCCGGGA CTTCAAGGGC
    AGCAGCCCGA AGCGTTCTGC AAGGAGCACC TTCCTGGGCT TGGAGGCCGA CTTGGGGTGC
    TGTGATAGTC TGCGGGCCTC TGCTTCCCAG GACAGCCTCC AGTTCAGTGA GGACGAGGCG
    CCCTACCCGC CAGGCTCCTC TGATAACGAT TATCTGAGTT TGCTCTATAA CTCCTTCGGC
    TGCAACTTGG CCCTAGCGGA TCGGTCCATC TCTGATAAAC TGTCTGAAGA AGTAGATGAA
    ATCTGGAATG ACTTGGAAAA CTACATCAAG AAAAACGAAG TCAAAGCCAG AAACCGGCTC
    CTGGCCGCGT TTCCGGTTAG CAAAGATGAC GTGCAGGAGC GGCCGCTGGC GGGCAGTACC
    CCGGAGCTGA GCGCGCGCTC CCCGCTCTCG CTGGCCGCGG GCCGGGCTCT CCTCGCGCCG
    CCGGCAGACA GGCCCGGGCC AGCCCCCTGC GCCCTGGACC GAGACCCAGC CTCCAAAGAC
    AGCTCCTGCC TGAGTCTCCA CCGGCTGTCG CTGGCCAGTG ACCTGCCGCC CGCAGAGAGT
    CCCTATGATG TGGCCGGCGG CAGCCTGTCC TCAGCGGACC TAGAGAACCC TGAGCCCGGG
    CTGGACAATG TAGACAAGAC CAGGAGCAGG GTTTTCCTGA TGGCCAGGCA GTACAGTCAG
    AAGATCAAGA AGGCAAATCA GCTGCTAAAA GTGAAAAACC CAGAGCTGGA ACAGACGCTC
    AGTAGCCAGC CGCAGAAGCC CGCGTCCAAG GACCTGGCGG CCATCCTAGA AGAGAAGAGG
    CAGGGCGGGC CCGCCATCGG TGCCAGGATT GCTGAGTATT CCCAACTGTA TGACCAGATT
    GTATTTAGAG AGACCCCCTT TAAGACTCAG AAGGATGGCT GGGCCAGCCC TCAAGACCCC
    TCCAACCTCA GCTCTGCATC ACCTTCCCAT GCCCAGCAAG GTAGCGAGGA CTGGCTTTTG
    CATTCAACCT ATAGTAATGG AGAGTTAGCT GACTTCTGTC CCTGGCCAGA ACAAGACTTA
    AAGTCAAAAT ATCCCATCTT AGAGATCAAT GCAAGAAGTA CCCCAAAACA ACTGTCTGCA
    GCTTGCTCTG TGCCTTCTCT TCAAACCTCC GACCCTCTGC TGGGCTTCGT GCAGAGACAC
    AGCGTGGTGG TCAGCCAGCC AAACAAAGAG AACTCCTGCC AGGGCCATCT TTACAACTCT
    CTGGGTCGGA AAGGAATCAG TGCTAAATCT CAGCCTTATA ACAGGTCCCA ATCATCTTCC
    TCCATCCTGA TAAACAAGTC TGCAGACTCC ATCAACTACC CTAGTGGCGT GGCAAAGCAG
    CAGCCTGTCT CTCTACACAG AAGTTCAAGG TGGGAGAGTC ACCAGGACTT GCTGCCAAGT
    ATTGCTGACT CAGGTCAACA GGGCCCTGAA AAACGCTCAG ATCTCACACT CCAGGACTCG
    CAGAAAGTTC TGGTAGTGAA TAGAAATTTA CCTTTAAGTG CTCAAATGGC TACCCAGAAC
    TATTTTTCCA ATTTCAAAGA GACTGAAGGA GACGAAGATG ACTATGTGGA GATCAAGTCA
    GAAGAAGATG AGTCTGAGTT AGAACTGTCT CACAGTCGGA GGAGGAAATC TGACCCAAAG
    ATTACAGACG CTGACTTTGC AGATAACGTC TGCAGCCACA CGACTTCTTA CTCTTTGAAT
    AGTCCGTGCA CTCCAAAAAA GCCAGTAAGT GGCAAACTTG GGATTTCGCC GTATCTGACG
    CCATACAATG ATCCCGACAA ACTGAATGAC TATCTCTGGA AGGGCCCATC TCCCAATCAG
    CAAAATATTG TGCAGTCTCT AAGGGAAAAA TTCCAGTGTC TTAGTTCAAG CAGCTTTGCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237778
    Clone ID Related Accession (Same CDS sequence) XM_024996655.1 , XM_024996658.1 , XM_024996654.1 , NM_001205921.2 , XM_024996656.1
    Accession Version XM_024996654.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4143bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pleckstrin homology domain-containing family G member 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAGCTCT CCGACAGTGA CCGCCCGGTC AGCTTCGGCT CCACGTCGTC CTCGGCTTCC 
    TCCCGGGACA GCCATGGTTC CTTCGGCAGC AGAATGACCT TAGTTTCAAG CAGCCATCTG
    GGCTTGTTTC CTCAGGATAA GGAAGCAGGG GCCATAAAAC TGGAGCTGAT GCCAGCCAGG
    CCGTTTTCCA GCAGCGAGCT GCAGACGGAC CGTGCGGCCG GGCAGCAGAG CACAGACGAG
    GGCGGCGAGA GGCAGCCCCG GGCACAGCGC AGAGTGGAAG CCAACGGGCC GACCAAAACC
    GGGGCCGAAT CGGCCACCAG CCCCAAGCTG CTCTATGTGG ACAGAGTTGT GCAGGAAATT
    CTGGAGACCG AACGGACTTA CGTGCAAGAT TTAAAGAGCA TCGTAGAGGA TTACCTTGAC
    TGTATCAGGG ACCAAACGAA ACTTCCTCTG GGGACAGATG AAAGATCAGC CCTTTTTGGA
    AACATACAGG ATATCTACCA CTTTAATAGT GAACTTCTGC AAGAGTTGGA AAACTGTGAA
    AATGATCCTG TGGCCATAGC AGAGTGCTTT GTGTCCAAGA GTGAAGAGTT CCACATTTAT
    ACCCAGTATT GCACGAACTA TCCAAGATCT GTGGCTGTGC TAACAGAGTG TATGAGGAAC
    AAGATGCTGG CCAAATTCTT CAGGGAACGG CAGGAAACTT TGAAACATTC ATTGCCTCTG
    GGGTCCTATC TCTTGAAGCC AGTTCAGCGG ATTCTCAAGT ATCATCTCCT TTTGCATGAA
    ATAGAAAATC ACCTTGACAA GGACACAGAG GGCTACGATG TGGTGCTTGA TGCCATAGAC
    ACGATGCAGA GAGTGGCCTG GCACATCAAT GACATGAAGC GGAAGCATGA ACACGCCGTC
    CGCCTGCAGG AGATACAGAG TTTGCTCACC AACTGGAAGG GGCCAGACCT GACCAGCTAC
    GGGGAGCTAG TGCTCGAAGG GACCTTCCGC CTCCAGCGGG CCAAGAATGA GCGGACGCTC
    TTCCTCTTTG ACAAGCTGCT GCTCATCACC AAGAAGAGAG ACGACACGTT TACATACAAA
    GCCCACATCC TGTGCGGCAA CCTCATGCTG GTGGAAGTGA TCCCAAAGGA GCCCCTCAGC
    TTCAGCGTCT TCCACTACAA GAACCCCAAG CTGCAGCACA CGGTTCAGGC GAAGTCGCAG
    CAAGACAAGC GCCTGTGGGT TCTGCACCTG AAGAGGCTGA TCCTGGAGAA TCATGCGGCA
    AAGATTCCAG CTAAGGCCAA GCAAGCAATA CTTGAAATGG ACGCCATTCA TTACCCAGGC
    TTCTGTTACA GTCCCGAGGG AGAGACTTGT GGCTCTCGAA ATGGCTCTGC TCCGTATCGG
    CTGAGAAGGA AATCTGAACC TTCCTCAAGA TCACATAAAG TTTTGAAGAC CAGTGAAACA
    GCACAAGACA TTCAAAAGGT TTCCAGAGAG GAAGGGTCTC CCCAGTTGAC TTCAGCAGGG
    CCCTCTCCTG CCCAGCGGAA CAGTCAGCCA AGCAGTTCGA CCATCCTGAG CGTGCTTCGT
    GGGGGTGGGG CTGTCAGAAA CATCTGGACC GACCACCAGA TCCGCCAAGC CCTGTTTCCC
    AGCCGAAGGT CGCAGGAGAA CGAGGAGGAT GAAGACGACT ATCAGATGTT CCTGCCATCC
    TTCTCCTCCT CGGATCTGAA CTCAGCCCAG CTGTGTGAAG ATGGTACTTC TAGTCGACCT
    TGCAGTTGGC ACATGGGACA AATCGAGTCC TCCGAGACCT CCAGCTCTGG CCACAGGATT
    GTGCGGCGGG CCAGCAGTGC TGGCGAGAGC AACACGTGTC CCCCTGACAT AAGAACTAGG
    GACAGTGGTG GTTCTCAGTA CAGCTCCCGG AGGGAACTGC AGGGTGACCC TAAAACAGAG
    GGGCAGGACG GGACAACTCC CTACGGGTCC TCTATAGAGT TGACTATAGA TGACATAGAC
    CATGTCTATG ACAACATAAG TTACGAGGAC TTGAAACTGA TGGTTGCTAA ACGCGAAGAC
    GCTGAAGTCA GTCCCCAGAA ACCAGTCAGG GACTCTGTTC GCCCCAAGAG CACCCCAGAG
    CTGGCCTTCT CCAAGAAGCC AGCGGGGCCT GAGGAGGAGA GCACATTGCA CCCCAGGAGA
    GATGGAGCCC TCCCGGCCGG GGAGGCCTGG AACCAAAGCA TGCACGACCT CCAGGTGATG
    GAGGAGAATG TCTATGACAC CATTGCGCTC CCGGAGGCGC CCTCCCGGGA CTTCAAGGGC
    AGCAGCCCGA AGCGTTCTGC AAGGAGCACC TTCCTGGGCT TGGAGGCCGA CTTGGGGTGC
    TGTGATAGTC TGCGGGCCTC TGCTTCCCAG GACAGCCTCC AGTTCAGTGA GGACGAGGCG
    CCCTACCCGC CAGGCTCCTC TGATAACGAT TATCTGAGTT TGCTCTATAA CTCCTTCGGC
    TGCAACTTGG CCCTAGCGGA TCGGTCCATC TCTGATAAAC TGTCTGAAGA AGTAGATGAA
    ATCTGGAATG ACTTGGAAAA CTACATCAAG AAAAACGAAG TCAAAGCCAG AAACCGGCTC
    CTGGCCGCGT TTCCGGTTAG CAAAGATGAC GTGCAGGAGC GGCCGCTGGC GGGCAGTACC
    CCGGAGCTGA GCGCGCGCTC CCCGCTCTCG CTGGCCGCGG GCCGGGCTCT CCTCGCGCCG
    CCGGCAGACA GGCCCGGGCC AGCCCCCTGC GCCCTGGACC GAGACCCAGC CTCCAAAGAC
    AGCTCCTGCC TGAGTCTCCA CCGGCTGTCG CTGGCCAGTG ACCTGCCGCC CGCAGAGAGT
    CCCTATGATG TGGCCGGCGG CAGCCTGTCC TCAGCGGACC TAGAGAACCC TGAGCCCGGG
    CTGGACAATG TAGACAAGAC CAGGAGCAGG GTTTTCCTGA TGGCCAGGCA GTACAGTCAG
    AAGATCAAGA AGGCAAATCA GCTGCTAAAA GTGAAAAACC CAGAGCTGGA ACAGACGCTC
    AGTAGCCAGC CGCAGAAGCC CGCGTCCAAG GACCTGGCGG CCATCCTAGA AGAGAAGAGG
    CAGGGCGGGC CCGCCATCGG TGCCAGGATT GCTGAGTATT CCCAACTGTA TGACCAGATT
    GTATTTAGAG AGACCCCCTT TAAGACTCAG AAGGATGGCT GGGCCAGCCC TCAAGACCCC
    TCCAACCTCA GCTCTGCATC ACCTTCCCAT GCCCAGCAAG GTAGCGAGGA CTGGCTTTTG
    CATTCAACCT ATAGTAATGG AGAGTTAGCT GACTTCTGTC CCTGGCCAGA ACAAGACTTA
    AAGTCAAAAT ATCCCATCTT AGAGATCAAT GCAAGAAGTA CCCCAAAACA ACTGTCTGCA
    GCTTGCTCTG TGCCTTCTCT TCAAACCTCC GACCCTCTGC TGGGCTTCGT GCAGAGACAC
    AGCGTGGTGG TCAGCCAGCC AAACAAAGAG AACTCCTGCC AGGGCCATCT TTACAACTCT
    CTGGGTCGGA AAGGAATCAG TGCTAAATCT CAGCCTTATA ACAGGTCCCA ATCATCTTCC
    TCCATCCTGA TAAACAAGTC TGCAGACTCC ATCAACTACC CTAGTGGCGT GGCAAAGCAG
    CAGCCTGTCT CTCTACACAG AAGTTCAAGG TGGGAGAGTC ACCAGGACTT GCTGCCAAGT
    ATTGCTGACT CAGGTCAACA GGGCCCTGAA AAACGCTCAG ATCTCACACT CCAGGACTCG
    CAGAAAGTTC TGGTAGTGAA TAGAAATTTA CCTTTAAGTG CTCAAATGGC TACCCAGAAC
    TATTTTTCCA ATTTCAAAGA GACTGAAGGA GACGAAGATG ACTATGTGGA GATCAAGTCA
    GAAGAAGATG AGTCTGAGTT AGAACTGTCT CACAGTCGGA GGAGGAAATC TGACCCAAAG
    ATTACAGACG CTGACTTTGC AGATAACGTC TGCAGCCACA CGACTTCTTA CTCTTTGAAT
    AGTCCGTGCA CTCCAAAAAA GCCAGTAAGT GGCAAACTTG GGATTTCGCC GTATCTGACG
    CCATACAATG ATCCCGACAA ACTGAATGAC TATCTCTGGA AGGGCCCATC TCCCAATCAG
    CAAAATATTG TGCAGTCTCT AAGGGAAAAA TTCCAGTGTC TTAGTTCAAG CAGCTTTGCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852422.1
    CDS295..4437
    Translation

    Target ORF information:

    RefSeq Version XM_024996654.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology and RhoGEF domain containing G1 (PLEKHG1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996654.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAGCTCT CCGACAGTGA CCGCCCGGTC AGCTTCGGCT CCACGTCGTC CTCGGCTTCC 
    TCCCGGGACA GCCATGGTTC CTTCGGCAGC AGAATGACCT TAGTTTCAAG CAGCCATCTG
    GGCTTGTTTC CTCAGGATAA GGAAGCAGGG GCCATAAAAC TGGAGCTGAT GCCAGCCAGG
    CCGTTTTCCA GCAGCGAGCT GCAGACGGAC CGTGCGGCCG GGCAGCAGAG CACAGACGAG
    GGCGGCGAGA GGCAGCCCCG GGCACAGCGC AGAGTGGAAG CCAACGGGCC GACCAAAACC
    GGGGCCGAAT CGGCCACCAG CCCCAAGCTG CTCTATGTGG ACAGAGTTGT GCAGGAAATT
    CTGGAGACCG AACGGACTTA CGTGCAAGAT TTAAAGAGCA TCGTAGAGGA TTACCTTGAC
    TGTATCAGGG ACCAAACGAA ACTTCCTCTG GGGACAGATG AAAGATCAGC CCTTTTTGGA
    AACATACAGG ATATCTACCA CTTTAATAGT GAACTTCTGC AAGAGTTGGA AAACTGTGAA
    AATGATCCTG TGGCCATAGC AGAGTGCTTT GTGTCCAAGA GTGAAGAGTT CCACATTTAT
    ACCCAGTATT GCACGAACTA TCCAAGATCT GTGGCTGTGC TAACAGAGTG TATGAGGAAC
    AAGATGCTGG CCAAATTCTT CAGGGAACGG CAGGAAACTT TGAAACATTC ATTGCCTCTG
    GGGTCCTATC TCTTGAAGCC AGTTCAGCGG ATTCTCAAGT ATCATCTCCT TTTGCATGAA
    ATAGAAAATC ACCTTGACAA GGACACAGAG GGCTACGATG TGGTGCTTGA TGCCATAGAC
    ACGATGCAGA GAGTGGCCTG GCACATCAAT GACATGAAGC GGAAGCATGA ACACGCCGTC
    CGCCTGCAGG AGATACAGAG TTTGCTCACC AACTGGAAGG GGCCAGACCT GACCAGCTAC
    GGGGAGCTAG TGCTCGAAGG GACCTTCCGC CTCCAGCGGG CCAAGAATGA GCGGACGCTC
    TTCCTCTTTG ACAAGCTGCT GCTCATCACC AAGAAGAGAG ACGACACGTT TACATACAAA
    GCCCACATCC TGTGCGGCAA CCTCATGCTG GTGGAAGTGA TCCCAAAGGA GCCCCTCAGC
    TTCAGCGTCT TCCACTACAA GAACCCCAAG CTGCAGCACA CGGTTCAGGC GAAGTCGCAG
    CAAGACAAGC GCCTGTGGGT TCTGCACCTG AAGAGGCTGA TCCTGGAGAA TCATGCGGCA
    AAGATTCCAG CTAAGGCCAA GCAAGCAATA CTTGAAATGG ACGCCATTCA TTACCCAGGC
    TTCTGTTACA GTCCCGAGGG AGAGACTTGT GGCTCTCGAA ATGGCTCTGC TCCGTATCGG
    CTGAGAAGGA AATCTGAACC TTCCTCAAGA TCACATAAAG TTTTGAAGAC CAGTGAAACA
    GCACAAGACA TTCAAAAGGT TTCCAGAGAG GAAGGGTCTC CCCAGTTGAC TTCAGCAGGG
    CCCTCTCCTG CCCAGCGGAA CAGTCAGCCA AGCAGTTCGA CCATCCTGAG CGTGCTTCGT
    GGGGGTGGGG CTGTCAGAAA CATCTGGACC GACCACCAGA TCCGCCAAGC CCTGTTTCCC
    AGCCGAAGGT CGCAGGAGAA CGAGGAGGAT GAAGACGACT ATCAGATGTT CCTGCCATCC
    TTCTCCTCCT CGGATCTGAA CTCAGCCCAG CTGTGTGAAG ATGGTACTTC TAGTCGACCT
    TGCAGTTGGC ACATGGGACA AATCGAGTCC TCCGAGACCT CCAGCTCTGG CCACAGGATT
    GTGCGGCGGG CCAGCAGTGC TGGCGAGAGC AACACGTGTC CCCCTGACAT AAGAACTAGG
    GACAGTGGTG GTTCTCAGTA CAGCTCCCGG AGGGAACTGC AGGGTGACCC TAAAACAGAG
    GGGCAGGACG GGACAACTCC CTACGGGTCC TCTATAGAGT TGACTATAGA TGACATAGAC
    CATGTCTATG ACAACATAAG TTACGAGGAC TTGAAACTGA TGGTTGCTAA ACGCGAAGAC
    GCTGAAGTCA GTCCCCAGAA ACCAGTCAGG GACTCTGTTC GCCCCAAGAG CACCCCAGAG
    CTGGCCTTCT CCAAGAAGCC AGCGGGGCCT GAGGAGGAGA GCACATTGCA CCCCAGGAGA
    GATGGAGCCC TCCCGGCCGG GGAGGCCTGG AACCAAAGCA TGCACGACCT CCAGGTGATG
    GAGGAGAATG TCTATGACAC CATTGCGCTC CCGGAGGCGC CCTCCCGGGA CTTCAAGGGC
    AGCAGCCCGA AGCGTTCTGC AAGGAGCACC TTCCTGGGCT TGGAGGCCGA CTTGGGGTGC
    TGTGATAGTC TGCGGGCCTC TGCTTCCCAG GACAGCCTCC AGTTCAGTGA GGACGAGGCG
    CCCTACCCGC CAGGCTCCTC TGATAACGAT TATCTGAGTT TGCTCTATAA CTCCTTCGGC
    TGCAACTTGG CCCTAGCGGA TCGGTCCATC TCTGATAAAC TGTCTGAAGA AGTAGATGAA
    ATCTGGAATG ACTTGGAAAA CTACATCAAG AAAAACGAAG TCAAAGCCAG AAACCGGCTC
    CTGGCCGCGT TTCCGGTTAG CAAAGATGAC GTGCAGGAGC GGCCGCTGGC GGGCAGTACC
    CCGGAGCTGA GCGCGCGCTC CCCGCTCTCG CTGGCCGCGG GCCGGGCTCT CCTCGCGCCG
    CCGGCAGACA GGCCCGGGCC AGCCCCCTGC GCCCTGGACC GAGACCCAGC CTCCAAAGAC
    AGCTCCTGCC TGAGTCTCCA CCGGCTGTCG CTGGCCAGTG ACCTGCCGCC CGCAGAGAGT
    CCCTATGATG TGGCCGGCGG CAGCCTGTCC TCAGCGGACC TAGAGAACCC TGAGCCCGGG
    CTGGACAATG TAGACAAGAC CAGGAGCAGG GTTTTCCTGA TGGCCAGGCA GTACAGTCAG
    AAGATCAAGA AGGCAAATCA GCTGCTAAAA GTGAAAAACC CAGAGCTGGA ACAGACGCTC
    AGTAGCCAGC CGCAGAAGCC CGCGTCCAAG GACCTGGCGG CCATCCTAGA AGAGAAGAGG
    CAGGGCGGGC CCGCCATCGG TGCCAGGATT GCTGAGTATT CCCAACTGTA TGACCAGATT
    GTATTTAGAG AGACCCCCTT TAAGACTCAG AAGGATGGCT GGGCCAGCCC TCAAGACCCC
    TCCAACCTCA GCTCTGCATC ACCTTCCCAT GCCCAGCAAG GTAGCGAGGA CTGGCTTTTG
    CATTCAACCT ATAGTAATGG AGAGTTAGCT GACTTCTGTC CCTGGCCAGA ACAAGACTTA
    AAGTCAAAAT ATCCCATCTT AGAGATCAAT GCAAGAAGTA CCCCAAAACA ACTGTCTGCA
    GCTTGCTCTG TGCCTTCTCT TCAAACCTCC GACCCTCTGC TGGGCTTCGT GCAGAGACAC
    AGCGTGGTGG TCAGCCAGCC AAACAAAGAG AACTCCTGCC AGGGCCATCT TTACAACTCT
    CTGGGTCGGA AAGGAATCAG TGCTAAATCT CAGCCTTATA ACAGGTCCCA ATCATCTTCC
    TCCATCCTGA TAAACAAGTC TGCAGACTCC ATCAACTACC CTAGTGGCGT GGCAAAGCAG
    CAGCCTGTCT CTCTACACAG AAGTTCAAGG TGGGAGAGTC ACCAGGACTT GCTGCCAAGT
    ATTGCTGACT CAGGTCAACA GGGCCCTGAA AAACGCTCAG ATCTCACACT CCAGGACTCG
    CAGAAAGTTC TGGTAGTGAA TAGAAATTTA CCTTTAAGTG CTCAAATGGC TACCCAGAAC
    TATTTTTCCA ATTTCAAAGA GACTGAAGGA GACGAAGATG ACTATGTGGA GATCAAGTCA
    GAAGAAGATG AGTCTGAGTT AGAACTGTCT CACAGTCGGA GGAGGAAATC TGACCCAAAG
    ATTACAGACG CTGACTTTGC AGATAACGTC TGCAGCCACA CGACTTCTTA CTCTTTGAAT
    AGTCCGTGCA CTCCAAAAAA GCCAGTAAGT GGCAAACTTG GGATTTCGCC GTATCTGACG
    CCATACAATG ATCCCGACAA ACTGAATGAC TATCTCTGGA AGGGCCCATC TCCCAATCAG
    CAAAATATTG TGCAGTCTCT AAGGGAAAAA TTCCAGTGTC TTAGTTCAAG CAGCTTTGCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237778
    Clone ID Related Accession (Same CDS sequence) XM_024996655.1 , XM_024996658.1 , XM_024996654.1 , NM_001205921.2 , XM_024996656.1
    Accession Version XM_024996656.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4143bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pleckstrin homology domain-containing family G member 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAGCTCT CCGACAGTGA CCGCCCGGTC AGCTTCGGCT CCACGTCGTC CTCGGCTTCC 
    TCCCGGGACA GCCATGGTTC CTTCGGCAGC AGAATGACCT TAGTTTCAAG CAGCCATCTG
    GGCTTGTTTC CTCAGGATAA GGAAGCAGGG GCCATAAAAC TGGAGCTGAT GCCAGCCAGG
    CCGTTTTCCA GCAGCGAGCT GCAGACGGAC CGTGCGGCCG GGCAGCAGAG CACAGACGAG
    GGCGGCGAGA GGCAGCCCCG GGCACAGCGC AGAGTGGAAG CCAACGGGCC GACCAAAACC
    GGGGCCGAAT CGGCCACCAG CCCCAAGCTG CTCTATGTGG ACAGAGTTGT GCAGGAAATT
    CTGGAGACCG AACGGACTTA CGTGCAAGAT TTAAAGAGCA TCGTAGAGGA TTACCTTGAC
    TGTATCAGGG ACCAAACGAA ACTTCCTCTG GGGACAGATG AAAGATCAGC CCTTTTTGGA
    AACATACAGG ATATCTACCA CTTTAATAGT GAACTTCTGC AAGAGTTGGA AAACTGTGAA
    AATGATCCTG TGGCCATAGC AGAGTGCTTT GTGTCCAAGA GTGAAGAGTT CCACATTTAT
    ACCCAGTATT GCACGAACTA TCCAAGATCT GTGGCTGTGC TAACAGAGTG TATGAGGAAC
    AAGATGCTGG CCAAATTCTT CAGGGAACGG CAGGAAACTT TGAAACATTC ATTGCCTCTG
    GGGTCCTATC TCTTGAAGCC AGTTCAGCGG ATTCTCAAGT ATCATCTCCT TTTGCATGAA
    ATAGAAAATC ACCTTGACAA GGACACAGAG GGCTACGATG TGGTGCTTGA TGCCATAGAC
    ACGATGCAGA GAGTGGCCTG GCACATCAAT GACATGAAGC GGAAGCATGA ACACGCCGTC
    CGCCTGCAGG AGATACAGAG TTTGCTCACC AACTGGAAGG GGCCAGACCT GACCAGCTAC
    GGGGAGCTAG TGCTCGAAGG GACCTTCCGC CTCCAGCGGG CCAAGAATGA GCGGACGCTC
    TTCCTCTTTG ACAAGCTGCT GCTCATCACC AAGAAGAGAG ACGACACGTT TACATACAAA
    GCCCACATCC TGTGCGGCAA CCTCATGCTG GTGGAAGTGA TCCCAAAGGA GCCCCTCAGC
    TTCAGCGTCT TCCACTACAA GAACCCCAAG CTGCAGCACA CGGTTCAGGC GAAGTCGCAG
    CAAGACAAGC GCCTGTGGGT TCTGCACCTG AAGAGGCTGA TCCTGGAGAA TCATGCGGCA
    AAGATTCCAG CTAAGGCCAA GCAAGCAATA CTTGAAATGG ACGCCATTCA TTACCCAGGC
    TTCTGTTACA GTCCCGAGGG AGAGACTTGT GGCTCTCGAA ATGGCTCTGC TCCGTATCGG
    CTGAGAAGGA AATCTGAACC TTCCTCAAGA TCACATAAAG TTTTGAAGAC CAGTGAAACA
    GCACAAGACA TTCAAAAGGT TTCCAGAGAG GAAGGGTCTC CCCAGTTGAC TTCAGCAGGG
    CCCTCTCCTG CCCAGCGGAA CAGTCAGCCA AGCAGTTCGA CCATCCTGAG CGTGCTTCGT
    GGGGGTGGGG CTGTCAGAAA CATCTGGACC GACCACCAGA TCCGCCAAGC CCTGTTTCCC
    AGCCGAAGGT CGCAGGAGAA CGAGGAGGAT GAAGACGACT ATCAGATGTT CCTGCCATCC
    TTCTCCTCCT CGGATCTGAA CTCAGCCCAG CTGTGTGAAG ATGGTACTTC TAGTCGACCT
    TGCAGTTGGC ACATGGGACA AATCGAGTCC TCCGAGACCT CCAGCTCTGG CCACAGGATT
    GTGCGGCGGG CCAGCAGTGC TGGCGAGAGC AACACGTGTC CCCCTGACAT AAGAACTAGG
    GACAGTGGTG GTTCTCAGTA CAGCTCCCGG AGGGAACTGC AGGGTGACCC TAAAACAGAG
    GGGCAGGACG GGACAACTCC CTACGGGTCC TCTATAGAGT TGACTATAGA TGACATAGAC
    CATGTCTATG ACAACATAAG TTACGAGGAC TTGAAACTGA TGGTTGCTAA ACGCGAAGAC
    GCTGAAGTCA GTCCCCAGAA ACCAGTCAGG GACTCTGTTC GCCCCAAGAG CACCCCAGAG
    CTGGCCTTCT CCAAGAAGCC AGCGGGGCCT GAGGAGGAGA GCACATTGCA CCCCAGGAGA
    GATGGAGCCC TCCCGGCCGG GGAGGCCTGG AACCAAAGCA TGCACGACCT CCAGGTGATG
    GAGGAGAATG TCTATGACAC CATTGCGCTC CCGGAGGCGC CCTCCCGGGA CTTCAAGGGC
    AGCAGCCCGA AGCGTTCTGC AAGGAGCACC TTCCTGGGCT TGGAGGCCGA CTTGGGGTGC
    TGTGATAGTC TGCGGGCCTC TGCTTCCCAG GACAGCCTCC AGTTCAGTGA GGACGAGGCG
    CCCTACCCGC CAGGCTCCTC TGATAACGAT TATCTGAGTT TGCTCTATAA CTCCTTCGGC
    TGCAACTTGG CCCTAGCGGA TCGGTCCATC TCTGATAAAC TGTCTGAAGA AGTAGATGAA
    ATCTGGAATG ACTTGGAAAA CTACATCAAG AAAAACGAAG TCAAAGCCAG AAACCGGCTC
    CTGGCCGCGT TTCCGGTTAG CAAAGATGAC GTGCAGGAGC GGCCGCTGGC GGGCAGTACC
    CCGGAGCTGA GCGCGCGCTC CCCGCTCTCG CTGGCCGCGG GCCGGGCTCT CCTCGCGCCG
    CCGGCAGACA GGCCCGGGCC AGCCCCCTGC GCCCTGGACC GAGACCCAGC CTCCAAAGAC
    AGCTCCTGCC TGAGTCTCCA CCGGCTGTCG CTGGCCAGTG ACCTGCCGCC CGCAGAGAGT
    CCCTATGATG TGGCCGGCGG CAGCCTGTCC TCAGCGGACC TAGAGAACCC TGAGCCCGGG
    CTGGACAATG TAGACAAGAC CAGGAGCAGG GTTTTCCTGA TGGCCAGGCA GTACAGTCAG
    AAGATCAAGA AGGCAAATCA GCTGCTAAAA GTGAAAAACC CAGAGCTGGA ACAGACGCTC
    AGTAGCCAGC CGCAGAAGCC CGCGTCCAAG GACCTGGCGG CCATCCTAGA AGAGAAGAGG
    CAGGGCGGGC CCGCCATCGG TGCCAGGATT GCTGAGTATT CCCAACTGTA TGACCAGATT
    GTATTTAGAG AGACCCCCTT TAAGACTCAG AAGGATGGCT GGGCCAGCCC TCAAGACCCC
    TCCAACCTCA GCTCTGCATC ACCTTCCCAT GCCCAGCAAG GTAGCGAGGA CTGGCTTTTG
    CATTCAACCT ATAGTAATGG AGAGTTAGCT GACTTCTGTC CCTGGCCAGA ACAAGACTTA
    AAGTCAAAAT ATCCCATCTT AGAGATCAAT GCAAGAAGTA CCCCAAAACA ACTGTCTGCA
    GCTTGCTCTG TGCCTTCTCT TCAAACCTCC GACCCTCTGC TGGGCTTCGT GCAGAGACAC
    AGCGTGGTGG TCAGCCAGCC AAACAAAGAG AACTCCTGCC AGGGCCATCT TTACAACTCT
    CTGGGTCGGA AAGGAATCAG TGCTAAATCT CAGCCTTATA ACAGGTCCCA ATCATCTTCC
    TCCATCCTGA TAAACAAGTC TGCAGACTCC ATCAACTACC CTAGTGGCGT GGCAAAGCAG
    CAGCCTGTCT CTCTACACAG AAGTTCAAGG TGGGAGAGTC ACCAGGACTT GCTGCCAAGT
    ATTGCTGACT CAGGTCAACA GGGCCCTGAA AAACGCTCAG ATCTCACACT CCAGGACTCG
    CAGAAAGTTC TGGTAGTGAA TAGAAATTTA CCTTTAAGTG CTCAAATGGC TACCCAGAAC
    TATTTTTCCA ATTTCAAAGA GACTGAAGGA GACGAAGATG ACTATGTGGA GATCAAGTCA
    GAAGAAGATG AGTCTGAGTT AGAACTGTCT CACAGTCGGA GGAGGAAATC TGACCCAAAG
    ATTACAGACG CTGACTTTGC AGATAACGTC TGCAGCCACA CGACTTCTTA CTCTTTGAAT
    AGTCCGTGCA CTCCAAAAAA GCCAGTAAGT GGCAAACTTG GGATTTCGCC GTATCTGACG
    CCATACAATG ATCCCGACAA ACTGAATGAC TATCTCTGGA AGGGCCCATC TCCCAATCAG
    CAAAATATTG TGCAGTCTCT AAGGGAAAAA TTCCAGTGTC TTAGTTCAAG CAGCTTTGCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852424.1
    CDS192..4334
    Translation

    Target ORF information:

    RefSeq Version XM_024996656.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology and RhoGEF domain containing G1 (PLEKHG1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996656.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAGCTCT CCGACAGTGA CCGCCCGGTC AGCTTCGGCT CCACGTCGTC CTCGGCTTCC 
    TCCCGGGACA GCCATGGTTC CTTCGGCAGC AGAATGACCT TAGTTTCAAG CAGCCATCTG
    GGCTTGTTTC CTCAGGATAA GGAAGCAGGG GCCATAAAAC TGGAGCTGAT GCCAGCCAGG
    CCGTTTTCCA GCAGCGAGCT GCAGACGGAC CGTGCGGCCG GGCAGCAGAG CACAGACGAG
    GGCGGCGAGA GGCAGCCCCG GGCACAGCGC AGAGTGGAAG CCAACGGGCC GACCAAAACC
    GGGGCCGAAT CGGCCACCAG CCCCAAGCTG CTCTATGTGG ACAGAGTTGT GCAGGAAATT
    CTGGAGACCG AACGGACTTA CGTGCAAGAT TTAAAGAGCA TCGTAGAGGA TTACCTTGAC
    TGTATCAGGG ACCAAACGAA ACTTCCTCTG GGGACAGATG AAAGATCAGC CCTTTTTGGA
    AACATACAGG ATATCTACCA CTTTAATAGT GAACTTCTGC AAGAGTTGGA AAACTGTGAA
    AATGATCCTG TGGCCATAGC AGAGTGCTTT GTGTCCAAGA GTGAAGAGTT CCACATTTAT
    ACCCAGTATT GCACGAACTA TCCAAGATCT GTGGCTGTGC TAACAGAGTG TATGAGGAAC
    AAGATGCTGG CCAAATTCTT CAGGGAACGG CAGGAAACTT TGAAACATTC ATTGCCTCTG
    GGGTCCTATC TCTTGAAGCC AGTTCAGCGG ATTCTCAAGT ATCATCTCCT TTTGCATGAA
    ATAGAAAATC ACCTTGACAA GGACACAGAG GGCTACGATG TGGTGCTTGA TGCCATAGAC
    ACGATGCAGA GAGTGGCCTG GCACATCAAT GACATGAAGC GGAAGCATGA ACACGCCGTC
    CGCCTGCAGG AGATACAGAG TTTGCTCACC AACTGGAAGG GGCCAGACCT GACCAGCTAC
    GGGGAGCTAG TGCTCGAAGG GACCTTCCGC CTCCAGCGGG CCAAGAATGA GCGGACGCTC
    TTCCTCTTTG ACAAGCTGCT GCTCATCACC AAGAAGAGAG ACGACACGTT TACATACAAA
    GCCCACATCC TGTGCGGCAA CCTCATGCTG GTGGAAGTGA TCCCAAAGGA GCCCCTCAGC
    TTCAGCGTCT TCCACTACAA GAACCCCAAG CTGCAGCACA CGGTTCAGGC GAAGTCGCAG
    CAAGACAAGC GCCTGTGGGT TCTGCACCTG AAGAGGCTGA TCCTGGAGAA TCATGCGGCA
    AAGATTCCAG CTAAGGCCAA GCAAGCAATA CTTGAAATGG ACGCCATTCA TTACCCAGGC
    TTCTGTTACA GTCCCGAGGG AGAGACTTGT GGCTCTCGAA ATGGCTCTGC TCCGTATCGG
    CTGAGAAGGA AATCTGAACC TTCCTCAAGA TCACATAAAG TTTTGAAGAC CAGTGAAACA
    GCACAAGACA TTCAAAAGGT TTCCAGAGAG GAAGGGTCTC CCCAGTTGAC TTCAGCAGGG
    CCCTCTCCTG CCCAGCGGAA CAGTCAGCCA AGCAGTTCGA CCATCCTGAG CGTGCTTCGT
    GGGGGTGGGG CTGTCAGAAA CATCTGGACC GACCACCAGA TCCGCCAAGC CCTGTTTCCC
    AGCCGAAGGT CGCAGGAGAA CGAGGAGGAT GAAGACGACT ATCAGATGTT CCTGCCATCC
    TTCTCCTCCT CGGATCTGAA CTCAGCCCAG CTGTGTGAAG ATGGTACTTC TAGTCGACCT
    TGCAGTTGGC ACATGGGACA AATCGAGTCC TCCGAGACCT CCAGCTCTGG CCACAGGATT
    GTGCGGCGGG CCAGCAGTGC TGGCGAGAGC AACACGTGTC CCCCTGACAT AAGAACTAGG
    GACAGTGGTG GTTCTCAGTA CAGCTCCCGG AGGGAACTGC AGGGTGACCC TAAAACAGAG
    GGGCAGGACG GGACAACTCC CTACGGGTCC TCTATAGAGT TGACTATAGA TGACATAGAC
    CATGTCTATG ACAACATAAG TTACGAGGAC TTGAAACTGA TGGTTGCTAA ACGCGAAGAC
    GCTGAAGTCA GTCCCCAGAA ACCAGTCAGG GACTCTGTTC GCCCCAAGAG CACCCCAGAG
    CTGGCCTTCT CCAAGAAGCC AGCGGGGCCT GAGGAGGAGA GCACATTGCA CCCCAGGAGA
    GATGGAGCCC TCCCGGCCGG GGAGGCCTGG AACCAAAGCA TGCACGACCT CCAGGTGATG
    GAGGAGAATG TCTATGACAC CATTGCGCTC CCGGAGGCGC CCTCCCGGGA CTTCAAGGGC
    AGCAGCCCGA AGCGTTCTGC AAGGAGCACC TTCCTGGGCT TGGAGGCCGA CTTGGGGTGC
    TGTGATAGTC TGCGGGCCTC TGCTTCCCAG GACAGCCTCC AGTTCAGTGA GGACGAGGCG
    CCCTACCCGC CAGGCTCCTC TGATAACGAT TATCTGAGTT TGCTCTATAA CTCCTTCGGC
    TGCAACTTGG CCCTAGCGGA TCGGTCCATC TCTGATAAAC TGTCTGAAGA AGTAGATGAA
    ATCTGGAATG ACTTGGAAAA CTACATCAAG AAAAACGAAG TCAAAGCCAG AAACCGGCTC
    CTGGCCGCGT TTCCGGTTAG CAAAGATGAC GTGCAGGAGC GGCCGCTGGC GGGCAGTACC
    CCGGAGCTGA GCGCGCGCTC CCCGCTCTCG CTGGCCGCGG GCCGGGCTCT CCTCGCGCCG
    CCGGCAGACA GGCCCGGGCC AGCCCCCTGC GCCCTGGACC GAGACCCAGC CTCCAAAGAC
    AGCTCCTGCC TGAGTCTCCA CCGGCTGTCG CTGGCCAGTG ACCTGCCGCC CGCAGAGAGT
    CCCTATGATG TGGCCGGCGG CAGCCTGTCC TCAGCGGACC TAGAGAACCC TGAGCCCGGG
    CTGGACAATG TAGACAAGAC CAGGAGCAGG GTTTTCCTGA TGGCCAGGCA GTACAGTCAG
    AAGATCAAGA AGGCAAATCA GCTGCTAAAA GTGAAAAACC CAGAGCTGGA ACAGACGCTC
    AGTAGCCAGC CGCAGAAGCC CGCGTCCAAG GACCTGGCGG CCATCCTAGA AGAGAAGAGG
    CAGGGCGGGC CCGCCATCGG TGCCAGGATT GCTGAGTATT CCCAACTGTA TGACCAGATT
    GTATTTAGAG AGACCCCCTT TAAGACTCAG AAGGATGGCT GGGCCAGCCC TCAAGACCCC
    TCCAACCTCA GCTCTGCATC ACCTTCCCAT GCCCAGCAAG GTAGCGAGGA CTGGCTTTTG
    CATTCAACCT ATAGTAATGG AGAGTTAGCT GACTTCTGTC CCTGGCCAGA ACAAGACTTA
    AAGTCAAAAT ATCCCATCTT AGAGATCAAT GCAAGAAGTA CCCCAAAACA ACTGTCTGCA
    GCTTGCTCTG TGCCTTCTCT TCAAACCTCC GACCCTCTGC TGGGCTTCGT GCAGAGACAC
    AGCGTGGTGG TCAGCCAGCC AAACAAAGAG AACTCCTGCC AGGGCCATCT TTACAACTCT
    CTGGGTCGGA AAGGAATCAG TGCTAAATCT CAGCCTTATA ACAGGTCCCA ATCATCTTCC
    TCCATCCTGA TAAACAAGTC TGCAGACTCC ATCAACTACC CTAGTGGCGT GGCAAAGCAG
    CAGCCTGTCT CTCTACACAG AAGTTCAAGG TGGGAGAGTC ACCAGGACTT GCTGCCAAGT
    ATTGCTGACT CAGGTCAACA GGGCCCTGAA AAACGCTCAG ATCTCACACT CCAGGACTCG
    CAGAAAGTTC TGGTAGTGAA TAGAAATTTA CCTTTAAGTG CTCAAATGGC TACCCAGAAC
    TATTTTTCCA ATTTCAAAGA GACTGAAGGA GACGAAGATG ACTATGTGGA GATCAAGTCA
    GAAGAAGATG AGTCTGAGTT AGAACTGTCT CACAGTCGGA GGAGGAAATC TGACCCAAAG
    ATTACAGACG CTGACTTTGC AGATAACGTC TGCAGCCACA CGACTTCTTA CTCTTTGAAT
    AGTCCGTGCA CTCCAAAAAA GCCAGTAAGT GGCAAACTTG GGATTTCGCC GTATCTGACG
    CCATACAATG ATCCCGACAA ACTGAATGAC TATCTCTGGA AGGGCCCATC TCCCAATCAG
    CAAAATATTG TGCAGTCTCT AAGGGAAAAA TTCCAGTGTC TTAGTTCAAG CAGCTTTGCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.