PLEKHG1 cDNA ORF clone, Canis lupus familiaris(Dog)

The following PLEKHG1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PLEKHG1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe00947 XM_005615507.2
Latest version!
Canis lupus familiaris pleckstrin homology domain containing, family G (with RhoGef domain) member 1 (PLEKHG1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OCe00948 XM_541152.6
Latest version!
Canis lupus familiaris pleckstrin homology and RhoGEF domain containing G1 (PLEKHG1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OCe00948 XM_005615510.3
Latest version!
Canis lupus familiaris pleckstrin homology and RhoGEF domain containing G1 (PLEKHG1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OCe00948 XM_005615508.3
Latest version!
Canis lupus familiaris pleckstrin homology and RhoGEF domain containing G1 (PLEKHG1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OCe00948 XM_005615509.2
Latest version!
Canis lupus familiaris pleckstrin homology domain containing, family G (with RhoGef domain) member 1 (PLEKHG1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OCe34452 XM_014112767.1
Latest version!
Canis lupus familiaris pleckstrin homology domain containing, family G (with RhoGef domain) member 1 (PLEKHG1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
View Old Accession Versions >>
OCe00948 XM_541152.5 Canis lupus familiaris pleckstrin homology domain containing, family G (with RhoGef domain) member 1 (PLEKHG1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OCe00948 XM_005615508.2 Canis lupus familiaris pleckstrin homology domain containing, family G (with RhoGef domain) member 1 (PLEKHG1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OCe00948 XM_005615510.2 Canis lupus familiaris pleckstrin homology domain containing, family G (with RhoGef domain) member 1 (PLEKHG1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe00947
    Clone ID Related Accession (Same CDS sequence) XM_005615507.2
    Accession Version XM_005615507.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4437bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product pleckstrin homology domain-containing family G member 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726046.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005615507.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGGACAGG CCCCCGAGGA CTACCCGGCC TCCCGGGGCC TGCGAACCGC GGCGGGAGTC 
    AGAGGCCGGG GCGCCGCGCC GGGCTCGGGG GACCCCCGCC CTGGTGAAGA CGGACATTGG
    GAGTCTGAGC ACAGACACTG GAAAGACTTC AGCTGTAAAT ATGTGCAGCA GTTGTGTCTT
    GAAGTGCCAT CAGCTGTGAC AGAAATTCCA GATCCCAAGA CCACAGCCCG GCACTCGGGC
    TCCCAGGACC TGAGGACCAT GGAGCTCTCG GACAGCGACC GCCCGGTCAG CTTCGGCTCC
    ACGTCGTCCT CGGCTTCTTC CCGGGACAGC CACGGTTCCT TCGGCAGCAG AATGACCTTA
    GTCTCAAACA GCCACTTGGG CTTGTTTCAC CAAGATAAGG AAGCCGGGGC CATAAAACTG
    GAGCTGATTC CAGCCAGGCC GCTTTCCAGC AGCGAGCTGC AGCGGGCCGG CCCCGCTGGG
    CCACAGGACA CGGGCGACGG CGGCGACAGG CAGCCCCGGG CCCAGCGGAG AATGGAAGCA
    AACGGGGCCA CCAGAGCAGG TGCAGAGTCG GCCACCAGCC CCAAGCTCCT GTATGTGGAT
    AGGGTTGTTC AGGAGATTCT GGAGACTGAG AGGACTTACG TGCAAGACTT GAAAAGCATC
    GTAGAGGATT ACCTTGACTG CATCAGGGAC CAAACAAAAC TTCCTCTGGG GACAGAAGAA
    AGATCAGCCC TCTTTGGAAA CATTCAGGAT ATCTACCATT TCAATAGTGA ACTTCTACAA
    GATTTGGAAA ACTGTGAAAA TGATCCTGTG GCCATTGCAG AATGTTTTGT ATCCAAGAGT
    GAAGAGTTCC ACATTTACAC GCAGTACTGC ACTAACTATC CAAGATCAGT GGCTGTGCTG
    ACAGAGTGCA TGCGGAACAA GATGCTGGCC AAATTCTTCA GGGAACGTCA GGAAACTTTG
    AAACACTCAC TGCCTTTGGG GTCCTATCTC TTGAAACCAG TTCAGCGGAT TCTCAAGTAT
    CATCTCCTTT TGCATGAAAT AGAAAATCAC CTTGACAAGG ACACAGAAGG GTATGATGTG
    GTGCTTGATG CAATAGACAC GATGCAGCGA GTAGCCTGGC ACATCAACGA CATGAAGAGG
    AAGCACGAAC ACGCAGTCCG GTTACAGGAG ATCCAGAGTC TGCTCACCAA CTGGAAGGGG
    CCAGACCTGA CCAGCTATGG GGAACTGGTG CTCGAAGGAA CCTTTCGCAT CCAGCGGGCC
    AAGAATGAGC GGACACTCTT CCTCTTTGAC AAGCTGCTGC TCATCACAAA GAAGAGAGAT
    GACACGTTTA CATACAAAGC TCACATCCTT TGTGGCAACC TCATGCTGGT GGAAGTGATA
    CCTAAGGAGC CGCTCAGCTT CAGCGTCTTC CACTATAAGA ACCCAAAGCT GCAGCACACA
    GTCCAGGCTA AATCCCAGCA GGACAAACGC CTCTGGGTTC TGCACCTGAA GAGACTGATC
    CTGGAGAACC ATGCGGCTAA GATTCCAGCT AAGGCCAAGC AAGCAATACT TGAAATGGAT
    GCCATTCATT ATCCCGGTTT CTGTTACAGT CCTGAGGGAG AGACGAAAGC ATTGTGTGGT
    TCTAAAGATG GTTCGGCTCC GTATCGGCTG AGAAGAAAAT CTGAACCTTC CTCAAGATCA
    CATAAAGTTT TGAAGACCAG TGAAGCAGCA CAAGACATCC AAAAGGTTTC CAGAGAGGAA
    GGGTCTCCCC AGCGGACCTC AACCCGGCCA TCTCCCGCCC AGAGGAACAA CCAGCCAAGC
    AGTTCTGCCA TTATCAACGT GCTTCGTGGG GGTGGAGCCA TCAGAAACAT CTGGACAGAC
    CACCAGATCA GACAAGCCTT ATTTCCCAGT CGTCGGTCCC CTCAGGAGAA CGAGGATGAT
    GAAGATGATT ATCAGATGTT CGTGCCGTCC TTCTCCTCCT CAGATCTGAA CTCTACCAGA
    CTGTGTGAAG ACAGCACTTC CAGTCGCCCT TGCAGTTGGC ACATGGGACA GATTGAGCCC
    ACAGAGACCA CCAGCTCCAG CCACCGGGTT GTCAGGCGGG CCAGTAGCGC TGGTGAGAGC
    AATACATGCA CTCCTGAAAT AAGAATTAGG GACAGTGGTG GCTCTCAGTA CAGCCCCCGA
    AGGGAACTAC AGAGTGACCC TAAAACAGAA GGGCAGGATG GAATGACTCC CTTTGGGTCA
    TCTATAGAGT TGACTATTGA TGATATAGAC CATGTCTATG ATAACATAAG TTACGAGGAC
    TTAAAACTAA TGGTTGCAAA ACGGGAAGAA GCTGAATCCA CACCCCTCAA AATAGCCAGG
    GACTCTGTTC GCCCCAAGAG CACCCCAGAG CTAGCCTTCT CAAAGAAGCA AGCTGGCCAC
    GAGAAGAGCT CTCTGCACAC AAAAAGGGAT GGAGCTCTAA CAGGTTGCCA GGCATCCAAC
    CAAAGCACAC ACGAACTCCA GATGGTTGAG GAAAACATCT ATGATACCAT AGGGCTCCCA
    GATGCACCCT CGCTGGATTT TAACTGCAGC AGCCTGAAGC GTCCCAAGAG GAGCACCTTC
    TTAGGTCTGG AAGCCGACTT TGCCTGCTGT GACAGTCTGA GGCCCTTCAT TTCCCAGGAT
    AGCCTCCAGT TCAGTGAGGA TGAAGCTCCT TACCACCAGG GGCCTTCTGA TAATGATTAT
    TTGAGTTTGC TCTATAACTC TTTCAGCTGT AACCTATCTA TAGGTGATAA ATCTATATCT
    GATAAGTTGT CTGAAGAAGT AGATGAAATC TGGAATGACC TGGAAAATTA CATCAAGAAA
    AACGAAGTCA AAGCTAGAGA CCGGCTCCTC GCAGCATTTC CTGTCAGCAA AGATGACGTG
    CCAGACAGGC TGCACTCCGA GAGCACACCT GAGCTGGGCA GAGATGCGGC GCATTCTGTG
    TCTACCCTCT CGCTTCCTGA AGGTCACACC TTCCTCACGC CCCTGAAAGA CAAGCCTGGC
    AGAGCTGGCC GGGCCAACTG CCCTTTTGAA GAGGACCTGA TTTCTAAAGA AGCCTCCTTT
    ATGAGTCTTA ACCGGCTGTC TTTGGCCAGT GAAACACCTC CCATGGAAAA CCCCTATGAC
    TTGGCCAACA GCAGCAGTCT GTCCCAATCA GACTTGGAAA TCCCTGACGC CGGGATGGAT
    GTCAGTGATA AGACAAGAAG CAGGGTTTTT ATGATGGCCA GGCAGTATAG TCAGAAGATC
    AAGAAGGCAA ATCAACTTTT AAAAGTGAGA AGCCCAGAGT TGGAACAGCC ACTGGCCAGC
    CAGCAGCATA AATCCACGCA CAAAGACCTG GCAGCCATCT TGGAAGAAAA GAAGCAAGGG
    GGTCCTGCCA TCGGTGCCAG GATCGCTGAG TATTCCCAGC TGTATGACCA GATTGTGTTC
    AGAGAGACTC CTTGTAAGAC TCAGAAGGAT GGCTGGGCCA GCCCTCAAGA GCCCTCCAAC
    CTCAGATCTA CATCATCTTC CCAGGCCCAA CTTAGCAGTG AGGATTGGCT TTTGCATTCA
    ACCTATAGTA ATGGAGAGTT AGTGGATTTC TGTCCCCGGC CAGAGCAAGA CTTGAAGTCA
    AAACATCCTA CTTTAGAGAT CAGTACAAAA AGCATTCCAA GACAGCTGTC CACAGCTTGC
    TCTGTGCCTT CTCTTCAAAC CTCTGACCCT CTGCTGTGCT CCTTGCAGAG ACACAGTGTG
    ATAGTCAGCC AGCCCAACAA AGATAACTCG TATCAGGGCC ACCTCTACAA TTCGTTGGGT
    CGGAAAAGCA TCAGTGCGAA ATCTCAGCCT TATAGCAGGT CTCAGTCCTC TTCTTCAATC
    TTGATAAACA AGTCGGTGGA CTCCATCAGC TACCCTGGTG AAATGGGAAA GAAACAGCCA
    CTGTCTATCC ACAAAAGTTC GAGGTGTGAG AGTCACCAGG ATTTGCTGCC AGCTATGGCT
    GACCCGTGTC AGCAGGGCCC TGAAAAACAC TCAGACCTCA CGCTCCGAGA CTCACAGAAG
    GTTCTGGTAG TCAATAGAAA TGTACCCTTA AATGCCCAAA TAGCCACACA GAACTATTTT
    TCCAATTTCA AAGAGACTGA AGGAGACGAA GACGACTATG TGGAAATAAG ATCTGAAGAA
    GACGAGTCAG AAGTAGAGCC ATCCCATGGT CGGAGGAAGA AATCTGACCC AAGGATTGCA
    GAAGCTGACT TTTCGGAGGA CCCCTGCACC AACAGCACCC CTTACTCTTT GAATAGTCCA
    TGCACTCCAA AAAAGCCGAT AAGTGACAAG CTTGGGATTT CACCCTACCT GGCATCGTAC
    AGCGATTCGG ACAAACTGAA CGACTATCTC TGGAGGGGCC CGTCTCCCAA CCAGCAAAAT
    ATTGTCCAGT CGCTGAGGGA AAAATTTCAG TGTCTCAGTT CAAGCAGCTT TGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005615564.1
    CDS166..4602
    Translation

    Target ORF information:

    RefSeq Version XM_005615507.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris pleckstrin homology domain containing, family G (with RhoGef domain) member 1 (PLEKHG1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005615507.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGGACAGG CCCCCGAGGA CTACCCGGCC TCCCGGGGCC TGCGAACCGC GGCGGGAGTC 
    AGAGGCCGGG GCGCCGCGCC GGGCTCGGGG GACCCCCGCC CTGGTGAAGA CGGACATTGG
    GAGTCTGAGC ACAGACACTG GAAAGACTTC AGCTGTAAAT ATGTGCAGCA GTTGTGTCTT
    GAAGTGCCAT CAGCTGTGAC AGAAATTCCA GATCCCAAGA CCACAGCCCG GCACTCGGGC
    TCCCAGGACC TGAGGACCAT GGAGCTCTCG GACAGCGACC GCCCGGTCAG CTTCGGCTCC
    ACGTCGTCCT CGGCTTCTTC CCGGGACAGC CACGGTTCCT TCGGCAGCAG AATGACCTTA
    GTCTCAAACA GCCACTTGGG CTTGTTTCAC CAAGATAAGG AAGCCGGGGC CATAAAACTG
    GAGCTGATTC CAGCCAGGCC GCTTTCCAGC AGCGAGCTGC AGCGGGCCGG CCCCGCTGGG
    CCACAGGACA CGGGCGACGG CGGCGACAGG CAGCCCCGGG CCCAGCGGAG AATGGAAGCA
    AACGGGGCCA CCAGAGCAGG TGCAGAGTCG GCCACCAGCC CCAAGCTCCT GTATGTGGAT
    AGGGTTGTTC AGGAGATTCT GGAGACTGAG AGGACTTACG TGCAAGACTT GAAAAGCATC
    GTAGAGGATT ACCTTGACTG CATCAGGGAC CAAACAAAAC TTCCTCTGGG GACAGAAGAA
    AGATCAGCCC TCTTTGGAAA CATTCAGGAT ATCTACCATT TCAATAGTGA ACTTCTACAA
    GATTTGGAAA ACTGTGAAAA TGATCCTGTG GCCATTGCAG AATGTTTTGT ATCCAAGAGT
    GAAGAGTTCC ACATTTACAC GCAGTACTGC ACTAACTATC CAAGATCAGT GGCTGTGCTG
    ACAGAGTGCA TGCGGAACAA GATGCTGGCC AAATTCTTCA GGGAACGTCA GGAAACTTTG
    AAACACTCAC TGCCTTTGGG GTCCTATCTC TTGAAACCAG TTCAGCGGAT TCTCAAGTAT
    CATCTCCTTT TGCATGAAAT AGAAAATCAC CTTGACAAGG ACACAGAAGG GTATGATGTG
    GTGCTTGATG CAATAGACAC GATGCAGCGA GTAGCCTGGC ACATCAACGA CATGAAGAGG
    AAGCACGAAC ACGCAGTCCG GTTACAGGAG ATCCAGAGTC TGCTCACCAA CTGGAAGGGG
    CCAGACCTGA CCAGCTATGG GGAACTGGTG CTCGAAGGAA CCTTTCGCAT CCAGCGGGCC
    AAGAATGAGC GGACACTCTT CCTCTTTGAC AAGCTGCTGC TCATCACAAA GAAGAGAGAT
    GACACGTTTA CATACAAAGC TCACATCCTT TGTGGCAACC TCATGCTGGT GGAAGTGATA
    CCTAAGGAGC CGCTCAGCTT CAGCGTCTTC CACTATAAGA ACCCAAAGCT GCAGCACACA
    GTCCAGGCTA AATCCCAGCA GGACAAACGC CTCTGGGTTC TGCACCTGAA GAGACTGATC
    CTGGAGAACC ATGCGGCTAA GATTCCAGCT AAGGCCAAGC AAGCAATACT TGAAATGGAT
    GCCATTCATT ATCCCGGTTT CTGTTACAGT CCTGAGGGAG AGACGAAAGC ATTGTGTGGT
    TCTAAAGATG GTTCGGCTCC GTATCGGCTG AGAAGAAAAT CTGAACCTTC CTCAAGATCA
    CATAAAGTTT TGAAGACCAG TGAAGCAGCA CAAGACATCC AAAAGGTTTC CAGAGAGGAA
    GGGTCTCCCC AGCGGACCTC AACCCGGCCA TCTCCCGCCC AGAGGAACAA CCAGCCAAGC
    AGTTCTGCCA TTATCAACGT GCTTCGTGGG GGTGGAGCCA TCAGAAACAT CTGGACAGAC
    CACCAGATCA GACAAGCCTT ATTTCCCAGT CGTCGGTCCC CTCAGGAGAA CGAGGATGAT
    GAAGATGATT ATCAGATGTT CGTGCCGTCC TTCTCCTCCT CAGATCTGAA CTCTACCAGA
    CTGTGTGAAG ACAGCACTTC CAGTCGCCCT TGCAGTTGGC ACATGGGACA GATTGAGCCC
    ACAGAGACCA CCAGCTCCAG CCACCGGGTT GTCAGGCGGG CCAGTAGCGC TGGTGAGAGC
    AATACATGCA CTCCTGAAAT AAGAATTAGG GACAGTGGTG GCTCTCAGTA CAGCCCCCGA
    AGGGAACTAC AGAGTGACCC TAAAACAGAA GGGCAGGATG GAATGACTCC CTTTGGGTCA
    TCTATAGAGT TGACTATTGA TGATATAGAC CATGTCTATG ATAACATAAG TTACGAGGAC
    TTAAAACTAA TGGTTGCAAA ACGGGAAGAA GCTGAATCCA CACCCCTCAA AATAGCCAGG
    GACTCTGTTC GCCCCAAGAG CACCCCAGAG CTAGCCTTCT CAAAGAAGCA AGCTGGCCAC
    GAGAAGAGCT CTCTGCACAC AAAAAGGGAT GGAGCTCTAA CAGGTTGCCA GGCATCCAAC
    CAAAGCACAC ACGAACTCCA GATGGTTGAG GAAAACATCT ATGATACCAT AGGGCTCCCA
    GATGCACCCT CGCTGGATTT TAACTGCAGC AGCCTGAAGC GTCCCAAGAG GAGCACCTTC
    TTAGGTCTGG AAGCCGACTT TGCCTGCTGT GACAGTCTGA GGCCCTTCAT TTCCCAGGAT
    AGCCTCCAGT TCAGTGAGGA TGAAGCTCCT TACCACCAGG GGCCTTCTGA TAATGATTAT
    TTGAGTTTGC TCTATAACTC TTTCAGCTGT AACCTATCTA TAGGTGATAA ATCTATATCT
    GATAAGTTGT CTGAAGAAGT AGATGAAATC TGGAATGACC TGGAAAATTA CATCAAGAAA
    AACGAAGTCA AAGCTAGAGA CCGGCTCCTC GCAGCATTTC CTGTCAGCAA AGATGACGTG
    CCAGACAGGC TGCACTCCGA GAGCACACCT GAGCTGGGCA GAGATGCGGC GCATTCTGTG
    TCTACCCTCT CGCTTCCTGA AGGTCACACC TTCCTCACGC CCCTGAAAGA CAAGCCTGGC
    AGAGCTGGCC GGGCCAACTG CCCTTTTGAA GAGGACCTGA TTTCTAAAGA AGCCTCCTTT
    ATGAGTCTTA ACCGGCTGTC TTTGGCCAGT GAAACACCTC CCATGGAAAA CCCCTATGAC
    TTGGCCAACA GCAGCAGTCT GTCCCAATCA GACTTGGAAA TCCCTGACGC CGGGATGGAT
    GTCAGTGATA AGACAAGAAG CAGGGTTTTT ATGATGGCCA GGCAGTATAG TCAGAAGATC
    AAGAAGGCAA ATCAACTTTT AAAAGTGAGA AGCCCAGAGT TGGAACAGCC ACTGGCCAGC
    CAGCAGCATA AATCCACGCA CAAAGACCTG GCAGCCATCT TGGAAGAAAA GAAGCAAGGG
    GGTCCTGCCA TCGGTGCCAG GATCGCTGAG TATTCCCAGC TGTATGACCA GATTGTGTTC
    AGAGAGACTC CTTGTAAGAC TCAGAAGGAT GGCTGGGCCA GCCCTCAAGA GCCCTCCAAC
    CTCAGATCTA CATCATCTTC CCAGGCCCAA CTTAGCAGTG AGGATTGGCT TTTGCATTCA
    ACCTATAGTA ATGGAGAGTT AGTGGATTTC TGTCCCCGGC CAGAGCAAGA CTTGAAGTCA
    AAACATCCTA CTTTAGAGAT CAGTACAAAA AGCATTCCAA GACAGCTGTC CACAGCTTGC
    TCTGTGCCTT CTCTTCAAAC CTCTGACCCT CTGCTGTGCT CCTTGCAGAG ACACAGTGTG
    ATAGTCAGCC AGCCCAACAA AGATAACTCG TATCAGGGCC ACCTCTACAA TTCGTTGGGT
    CGGAAAAGCA TCAGTGCGAA ATCTCAGCCT TATAGCAGGT CTCAGTCCTC TTCTTCAATC
    TTGATAAACA AGTCGGTGGA CTCCATCAGC TACCCTGGTG AAATGGGAAA GAAACAGCCA
    CTGTCTATCC ACAAAAGTTC GAGGTGTGAG AGTCACCAGG ATTTGCTGCC AGCTATGGCT
    GACCCGTGTC AGCAGGGCCC TGAAAAACAC TCAGACCTCA CGCTCCGAGA CTCACAGAAG
    GTTCTGGTAG TCAATAGAAA TGTACCCTTA AATGCCCAAA TAGCCACACA GAACTATTTT
    TCCAATTTCA AAGAGACTGA AGGAGACGAA GACGACTATG TGGAAATAAG ATCTGAAGAA
    GACGAGTCAG AAGTAGAGCC ATCCCATGGT CGGAGGAAGA AATCTGACCC AAGGATTGCA
    GAAGCTGACT TTTCGGAGGA CCCCTGCACC AACAGCACCC CTTACTCTTT GAATAGTCCA
    TGCACTCCAA AAAAGCCGAT AAGTGACAAG CTTGGGATTT CACCCTACCT GGCATCGTAC
    AGCGATTCGG ACAAACTGAA CGACTATCTC TGGAGGGGCC CGTCTCCCAA CCAGCAAAAT
    ATTGTCCAGT CGCTGAGGGA AAAATTTCAG TGTCTCAGTT CAAGCAGCTT TGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe00948
    Clone ID Related Accession (Same CDS sequence) XM_541152.6 , XM_005615510.3 , XM_005615508.3 , XM_541152.5 , XM_005615508.2 , XM_005615509.2 , XM_005615510.2
    Accession Version XM_541152.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4179bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product pleckstrin homology domain-containing family G member 1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_541152.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAGCTCT CGGACAGCGA CCGCCCGGTC AGCTTCGGCT CCACGTCGTC CTCGGCTTCT 
    TCCCGGGACA GCCACGGTTC CTTCGGCAGC AGAATGACCT TAGTCTCAAA CAGCCACTTG
    GGCTTGTTTC ACCAAGATAA GGAAGCCGGG GCCATAAAAC TGGAGCTGAT TCCAGCCAGG
    CCGCTTTCCA GCAGCGAGCT GCAGCGGGCC GGCCCCGCTG GGCCACAGGA CACGGGCGAC
    GGCGGCGACA GGCAGCCCCG GGCCCAGCGG AGAATGGAAG CAAACGGGGC CACCAGAGCA
    GGTGCAGAGT CGGCCACCAG CCCCAAGCTC CTGTATGTGG ATAGGGTTGT TCAGGAGATT
    CTGGAGACTG AGAGGACTTA CGTGCAAGAC TTGAAAAGCA TCGTAGAGGA TTACCTTGAC
    TGCATCAGGG ACCAAACAAA ACTTCCTCTG GGGACAGAAG AAAGATCAGC CCTCTTTGGA
    AACATTCAGG ATATCTACCA TTTCAATAGT GAACTTCTAC AAGATTTGGA AAACTGTGAA
    AATGATCCTG TGGCCATTGC AGAATGTTTT GTATCCAAGA GTGAAGAGTT CCACATTTAC
    ACGCAGTACT GCACTAACTA TCCAAGATCA GTGGCTGTGC TGACAGAGTG CATGCGGAAC
    AAGATGCTGG CCAAATTCTT CAGGGAACGT CAGGAAACTT TGAAACACTC ACTGCCTTTG
    GGGTCCTATC TCTTGAAACC AGTTCAGCGG ATTCTCAAGT ATCATCTCCT TTTGCATGAA
    ATAGAAAATC ACCTTGACAA GGACACAGAA GGGTATGATG TGGTGCTTGA TGCAATAGAC
    ACGATGCAGC GAGTAGCCTG GCACATCAAC GACATGAAGA GGAAGCACGA ACACGCAGTC
    CGGTTACAGG AGATCCAGAG TCTGCTCACC AACTGGAAGG GGCCAGACCT GACCAGCTAT
    GGGGAACTGG TGCTCGAAGG AACCTTTCGC ATCCAGCGGG CCAAGAATGA GCGGACACTC
    TTCCTCTTTG ACAAGCTGCT GCTCATCACA AAGAAGAGAG ATGACACGTT TACATACAAA
    GCTCACATCC TTTGTGGCAA CCTCATGCTG GTGGAAGTGA TACCTAAGGA GCCGCTCAGC
    TTCAGCGTCT TCCACTATAA GAACCCAAAG CTGCAGCACA CAGTCCAGGC TAAATCCCAG
    CAGGACAAAC GCCTCTGGGT TCTGCACCTG AAGAGACTGA TCCTGGAGAA CCATGCGGCT
    AAGATTCCAG CTAAGGCCAA GCAAGCAATA CTTGAAATGG ATGCCATTCA TTATCCCGGT
    TTCTGTTACA GTCCTGAGGG AGAGACGAAA GCATTGTGTG GTTCTAAAGA TGGTTCGGCT
    CCGTATCGGC TGAGAAGAAA ATCTGAACCT TCCTCAAGAT CACATAAAGT TTTGAAGACC
    AGTGAAGCAG CACAAGACAT CCAAAAGGTT TCCAGAGAGG AAGGGTCTCC CCAGCGGACC
    TCAACCCGGC CATCTCCCGC CCAGAGGAAC AACCAGCCAA GCAGTTCTGC CATTATCAAC
    GTGCTTCGTG GGGGTGGAGC CATCAGAAAC ATCTGGACAG ACCACCAGAT CAGACAAGCC
    TTATTTCCCA GTCGTCGGTC CCCTCAGGAG AACGAGGATG ATGAAGATGA TTATCAGATG
    TTCGTGCCGT CCTTCTCCTC CTCAGATCTG AACTCTACCA GACTGTGTGA AGACAGCACT
    TCCAGTCGCC CTTGCAGTTG GCACATGGGA CAGATTGAGC CCACAGAGAC CACCAGCTCC
    AGCCACCGGG TTGTCAGGCG GGCCAGTAGC GCTGGTGAGA GCAATACATG CACTCCTGAA
    ATAAGAATTA GGGACAGTGG TGGCTCTCAG TACAGCCCCC GAAGGGAACT ACAGAGTGAC
    CCTAAAACAG AAGGGCAGGA TGGAATGACT CCCTTTGGGT CATCTATAGA GTTGACTATT
    GATGATATAG ACCATGTCTA TGATAACATA AGTTACGAGG ACTTAAAACT AATGGTTGCA
    AAACGGGAAG AAGCTGAATC CACACCCCTC AAAATAGCCA GGGACTCTGT TCGCCCCAAG
    AGCACCCCAG AGCTAGCCTT CTCAAAGAAG CAAGCTGGCC ACGAGAAGAG CTCTCTGCAC
    ACAAAAAGGG ATGGAGCTCT AACAGGTTGC CAGGCATCCA ACCAAAGCAC ACACGAACTC
    CAGATGGTTG AGGAAAACAT CTATGATACC ATAGGGCTCC CAGATGCACC CTCGCTGGAT
    TTTAACTGCA GCAGCCTGAA GCGTCCCAAG AGGAGCACCT TCTTAGGTCT GGAAGCCGAC
    TTTGCCTGCT GTGACAGTCT GAGGCCCTTC ATTTCCCAGG ATAGCCTCCA GTTCAGTGAG
    GATGAAGCTC CTTACCACCA GGGGCCTTCT GATAATGATT ATTTGAGTTT GCTCTATAAC
    TCTTTCAGCT GTAACCTATC TATAGGTGAT AAATCTATAT CTGATAAGTT GTCTGAAGAA
    GTAGATGAAA TCTGGAATGA CCTGGAAAAT TACATCAAGA AAAACGAAGT CAAAGCTAGA
    GACCGGCTCC TCGCAGCATT TCCTGTCAGC AAAGATGACG TGCCAGACAG GCTGCACTCC
    GAGAGCACAC CTGAGCTGGG CAGAGATGCG GCGCATTCTG TGTCTACCCT CTCGCTTCCT
    GAAGGTCACA CCTTCCTCAC GCCCCTGAAA GACAAGCCTG GCAGAGCTGG CCGGGCCAAC
    TGCCCTTTTG AAGAGGACCT GATTTCTAAA GAAGCCTCCT TTATGAGTCT TAACCGGCTG
    TCTTTGGCCA GTGAAACACC TCCCATGGAA AACCCCTATG ACTTGGCCAA CAGCAGCAGT
    CTGTCCCAAT CAGACTTGGA AATCCCTGAC GCCGGGATGG ATGTCAGTGA TAAGACAAGA
    AGCAGGGTTT TTATGATGGC CAGGCAGTAT AGTCAGAAGA TCAAGAAGGC AAATCAACTT
    TTAAAAGTGA GAAGCCCAGA GTTGGAACAG CCACTGGCCA GCCAGCAGCA TAAATCCACG
    CACAAAGACC TGGCAGCCAT CTTGGAAGAA AAGAAGCAAG GGGGTCCTGC CATCGGTGCC
    AGGATCGCTG AGTATTCCCA GCTGTATGAC CAGATTGTGT TCAGAGAGAC TCCTTGTAAG
    ACTCAGAAGG ATGGCTGGGC CAGCCCTCAA GAGCCCTCCA ACCTCAGATC TACATCATCT
    TCCCAGGCCC AACTTAGCAG TGAGGATTGG CTTTTGCATT CAACCTATAG TAATGGAGAG
    TTAGTGGATT TCTGTCCCCG GCCAGAGCAA GACTTGAAGT CAAAACATCC TACTTTAGAG
    ATCAGTACAA AAAGCATTCC AAGACAGCTG TCCACAGCTT GCTCTGTGCC TTCTCTTCAA
    ACCTCTGACC CTCTGCTGTG CTCCTTGCAG AGACACAGTG TGATAGTCAG CCAGCCCAAC
    AAAGATAACT CGTATCAGGG CCACCTCTAC AATTCGTTGG GTCGGAAAAG CATCAGTGCG
    AAATCTCAGC CTTATAGCAG GTCTCAGTCC TCTTCTTCAA TCTTGATAAA CAAGTCGGTG
    GACTCCATCA GCTACCCTGG TGAAATGGGA AAGAAACAGC CACTGTCTAT CCACAAAAGT
    TCGAGGTGTG AGAGTCACCA GGATTTGCTG CCAGCTATGG CTGACCCGTG TCAGCAGGGC
    CCTGAAAAAC ACTCAGACCT CACGCTCCGA GACTCACAGA AGGTTCTGGT AGTCAATAGA
    AATGTACCCT TAAATGCCCA AATAGCCACA CAGAACTATT TTTCCAATTT CAAAGAGACT
    GAAGGAGACG AAGACGACTA TGTGGAAATA AGATCTGAAG AAGACGAGTC AGAAGTAGAG
    CCATCCCATG GTCGGAGGAA GAAATCTGAC CCAAGGATTG CAGAAGCTGA CTTTTCGGAG
    GACCCCTGCA CCAACAGCAC CCCTTACTCT TTGAATAGTC CATGCACTCC AAAAAAGCCG
    ATAAGTGACA AGCTTGGGAT TTCACCCTAC CTGGCATCGT ACAGCGATTC GGACAAACTG
    AACGACTATC TCTGGAGGGG CCCGTCTCCC AACCAGCAAA ATATTGTCCA GTCGCTGAGG
    GAAAAATTTC AGTGTCTCAG TTCAAGCAGC TTTGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_541152.3
    CDS237..4415
    Translation

    Target ORF information:

    RefSeq Version XM_541152.6
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris pleckstrin homology and RhoGEF domain containing G1 (PLEKHG1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_541152.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAGCTCT CGGACAGCGA CCGCCCGGTC AGCTTCGGCT CCACGTCGTC CTCGGCTTCT 
    TCCCGGGACA GCCACGGTTC CTTCGGCAGC AGAATGACCT TAGTCTCAAA CAGCCACTTG
    GGCTTGTTTC ACCAAGATAA GGAAGCCGGG GCCATAAAAC TGGAGCTGAT TCCAGCCAGG
    CCGCTTTCCA GCAGCGAGCT GCAGCGGGCC GGCCCCGCTG GGCCACAGGA CACGGGCGAC
    GGCGGCGACA GGCAGCCCCG GGCCCAGCGG AGAATGGAAG CAAACGGGGC CACCAGAGCA
    GGTGCAGAGT CGGCCACCAG CCCCAAGCTC CTGTATGTGG ATAGGGTTGT TCAGGAGATT
    CTGGAGACTG AGAGGACTTA CGTGCAAGAC TTGAAAAGCA TCGTAGAGGA TTACCTTGAC
    TGCATCAGGG ACCAAACAAA ACTTCCTCTG GGGACAGAAG AAAGATCAGC CCTCTTTGGA
    AACATTCAGG ATATCTACCA TTTCAATAGT GAACTTCTAC AAGATTTGGA AAACTGTGAA
    AATGATCCTG TGGCCATTGC AGAATGTTTT GTATCCAAGA GTGAAGAGTT CCACATTTAC
    ACGCAGTACT GCACTAACTA TCCAAGATCA GTGGCTGTGC TGACAGAGTG CATGCGGAAC
    AAGATGCTGG CCAAATTCTT CAGGGAACGT CAGGAAACTT TGAAACACTC ACTGCCTTTG
    GGGTCCTATC TCTTGAAACC AGTTCAGCGG ATTCTCAAGT ATCATCTCCT TTTGCATGAA
    ATAGAAAATC ACCTTGACAA GGACACAGAA GGGTATGATG TGGTGCTTGA TGCAATAGAC
    ACGATGCAGC GAGTAGCCTG GCACATCAAC GACATGAAGA GGAAGCACGA ACACGCAGTC
    CGGTTACAGG AGATCCAGAG TCTGCTCACC AACTGGAAGG GGCCAGACCT GACCAGCTAT
    GGGGAACTGG TGCTCGAAGG AACCTTTCGC ATCCAGCGGG CCAAGAATGA GCGGACACTC
    TTCCTCTTTG ACAAGCTGCT GCTCATCACA AAGAAGAGAG ATGACACGTT TACATACAAA
    GCTCACATCC TTTGTGGCAA CCTCATGCTG GTGGAAGTGA TACCTAAGGA GCCGCTCAGC
    TTCAGCGTCT TCCACTATAA GAACCCAAAG CTGCAGCACA CAGTCCAGGC TAAATCCCAG
    CAGGACAAAC GCCTCTGGGT TCTGCACCTG AAGAGACTGA TCCTGGAGAA CCATGCGGCT
    AAGATTCCAG CTAAGGCCAA GCAAGCAATA CTTGAAATGG ATGCCATTCA TTATCCCGGT
    TTCTGTTACA GTCCTGAGGG AGAGACGAAA GCATTGTGTG GTTCTAAAGA TGGTTCGGCT
    CCGTATCGGC TGAGAAGAAA ATCTGAACCT TCCTCAAGAT CACATAAAGT TTTGAAGACC
    AGTGAAGCAG CACAAGACAT CCAAAAGGTT TCCAGAGAGG AAGGGTCTCC CCAGCGGACC
    TCAACCCGGC CATCTCCCGC CCAGAGGAAC AACCAGCCAA GCAGTTCTGC CATTATCAAC
    GTGCTTCGTG GGGGTGGAGC CATCAGAAAC ATCTGGACAG ACCACCAGAT CAGACAAGCC
    TTATTTCCCA GTCGTCGGTC CCCTCAGGAG AACGAGGATG ATGAAGATGA TTATCAGATG
    TTCGTGCCGT CCTTCTCCTC CTCAGATCTG AACTCTACCA GACTGTGTGA AGACAGCACT
    TCCAGTCGCC CTTGCAGTTG GCACATGGGA CAGATTGAGC CCACAGAGAC CACCAGCTCC
    AGCCACCGGG TTGTCAGGCG GGCCAGTAGC GCTGGTGAGA GCAATACATG CACTCCTGAA
    ATAAGAATTA GGGACAGTGG TGGCTCTCAG TACAGCCCCC GAAGGGAACT ACAGAGTGAC
    CCTAAAACAG AAGGGCAGGA TGGAATGACT CCCTTTGGGT CATCTATAGA GTTGACTATT
    GATGATATAG ACCATGTCTA TGATAACATA AGTTACGAGG ACTTAAAACT AATGGTTGCA
    AAACGGGAAG AAGCTGAATC CACACCCCTC AAAATAGCCA GGGACTCTGT TCGCCCCAAG
    AGCACCCCAG AGCTAGCCTT CTCAAAGAAG CAAGCTGGCC ACGAGAAGAG CTCTCTGCAC
    ACAAAAAGGG ATGGAGCTCT AACAGGTTGC CAGGCATCCA ACCAAAGCAC ACACGAACTC
    CAGATGGTTG AGGAAAACAT CTATGATACC ATAGGGCTCC CAGATGCACC CTCGCTGGAT
    TTTAACTGCA GCAGCCTGAA GCGTCCCAAG AGGAGCACCT TCTTAGGTCT GGAAGCCGAC
    TTTGCCTGCT GTGACAGTCT GAGGCCCTTC ATTTCCCAGG ATAGCCTCCA GTTCAGTGAG
    GATGAAGCTC CTTACCACCA GGGGCCTTCT GATAATGATT ATTTGAGTTT GCTCTATAAC
    TCTTTCAGCT GTAACCTATC TATAGGTGAT AAATCTATAT CTGATAAGTT GTCTGAAGAA
    GTAGATGAAA TCTGGAATGA CCTGGAAAAT TACATCAAGA AAAACGAAGT CAAAGCTAGA
    GACCGGCTCC TCGCAGCATT TCCTGTCAGC AAAGATGACG TGCCAGACAG GCTGCACTCC
    GAGAGCACAC CTGAGCTGGG CAGAGATGCG GCGCATTCTG TGTCTACCCT CTCGCTTCCT
    GAAGGTCACA CCTTCCTCAC GCCCCTGAAA GACAAGCCTG GCAGAGCTGG CCGGGCCAAC
    TGCCCTTTTG AAGAGGACCT GATTTCTAAA GAAGCCTCCT TTATGAGTCT TAACCGGCTG
    TCTTTGGCCA GTGAAACACC TCCCATGGAA AACCCCTATG ACTTGGCCAA CAGCAGCAGT
    CTGTCCCAAT CAGACTTGGA AATCCCTGAC GCCGGGATGG ATGTCAGTGA TAAGACAAGA
    AGCAGGGTTT TTATGATGGC CAGGCAGTAT AGTCAGAAGA TCAAGAAGGC AAATCAACTT
    TTAAAAGTGA GAAGCCCAGA GTTGGAACAG CCACTGGCCA GCCAGCAGCA TAAATCCACG
    CACAAAGACC TGGCAGCCAT CTTGGAAGAA AAGAAGCAAG GGGGTCCTGC CATCGGTGCC
    AGGATCGCTG AGTATTCCCA GCTGTATGAC CAGATTGTGT TCAGAGAGAC TCCTTGTAAG
    ACTCAGAAGG ATGGCTGGGC CAGCCCTCAA GAGCCCTCCA ACCTCAGATC TACATCATCT
    TCCCAGGCCC AACTTAGCAG TGAGGATTGG CTTTTGCATT CAACCTATAG TAATGGAGAG
    TTAGTGGATT TCTGTCCCCG GCCAGAGCAA GACTTGAAGT CAAAACATCC TACTTTAGAG
    ATCAGTACAA AAAGCATTCC AAGACAGCTG TCCACAGCTT GCTCTGTGCC TTCTCTTCAA
    ACCTCTGACC CTCTGCTGTG CTCCTTGCAG AGACACAGTG TGATAGTCAG CCAGCCCAAC
    AAAGATAACT CGTATCAGGG CCACCTCTAC AATTCGTTGG GTCGGAAAAG CATCAGTGCG
    AAATCTCAGC CTTATAGCAG GTCTCAGTCC TCTTCTTCAA TCTTGATAAA CAAGTCGGTG
    GACTCCATCA GCTACCCTGG TGAAATGGGA AAGAAACAGC CACTGTCTAT CCACAAAAGT
    TCGAGGTGTG AGAGTCACCA GGATTTGCTG CCAGCTATGG CTGACCCGTG TCAGCAGGGC
    CCTGAAAAAC ACTCAGACCT CACGCTCCGA GACTCACAGA AGGTTCTGGT AGTCAATAGA
    AATGTACCCT TAAATGCCCA AATAGCCACA CAGAACTATT TTTCCAATTT CAAAGAGACT
    GAAGGAGACG AAGACGACTA TGTGGAAATA AGATCTGAAG AAGACGAGTC AGAAGTAGAG
    CCATCCCATG GTCGGAGGAA GAAATCTGAC CCAAGGATTG CAGAAGCTGA CTTTTCGGAG
    GACCCCTGCA CCAACAGCAC CCCTTACTCT TTGAATAGTC CATGCACTCC AAAAAAGCCG
    ATAAGTGACA AGCTTGGGAT TTCACCCTAC CTGGCATCGT ACAGCGATTC GGACAAACTG
    AACGACTATC TCTGGAGGGG CCCGTCTCCC AACCAGCAAA ATATTGTCCA GTCGCTGAGG
    GAAAAATTTC AGTGTCTCAG TTCAAGCAGC TTTGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe00948
    Clone ID Related Accession (Same CDS sequence) XM_541152.6 , XM_005615510.3 , XM_005615508.3 , XM_541152.5 , XM_005615508.2 , XM_005615509.2 , XM_005615510.2
    Accession Version XM_005615510.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4179bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product pleckstrin homology domain-containing family G member 1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005615510.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAGCTCT CGGACAGCGA CCGCCCGGTC AGCTTCGGCT CCACGTCGTC CTCGGCTTCT 
    TCCCGGGACA GCCACGGTTC CTTCGGCAGC AGAATGACCT TAGTCTCAAA CAGCCACTTG
    GGCTTGTTTC ACCAAGATAA GGAAGCCGGG GCCATAAAAC TGGAGCTGAT TCCAGCCAGG
    CCGCTTTCCA GCAGCGAGCT GCAGCGGGCC GGCCCCGCTG GGCCACAGGA CACGGGCGAC
    GGCGGCGACA GGCAGCCCCG GGCCCAGCGG AGAATGGAAG CAAACGGGGC CACCAGAGCA
    GGTGCAGAGT CGGCCACCAG CCCCAAGCTC CTGTATGTGG ATAGGGTTGT TCAGGAGATT
    CTGGAGACTG AGAGGACTTA CGTGCAAGAC TTGAAAAGCA TCGTAGAGGA TTACCTTGAC
    TGCATCAGGG ACCAAACAAA ACTTCCTCTG GGGACAGAAG AAAGATCAGC CCTCTTTGGA
    AACATTCAGG ATATCTACCA TTTCAATAGT GAACTTCTAC AAGATTTGGA AAACTGTGAA
    AATGATCCTG TGGCCATTGC AGAATGTTTT GTATCCAAGA GTGAAGAGTT CCACATTTAC
    ACGCAGTACT GCACTAACTA TCCAAGATCA GTGGCTGTGC TGACAGAGTG CATGCGGAAC
    AAGATGCTGG CCAAATTCTT CAGGGAACGT CAGGAAACTT TGAAACACTC ACTGCCTTTG
    GGGTCCTATC TCTTGAAACC AGTTCAGCGG ATTCTCAAGT ATCATCTCCT TTTGCATGAA
    ATAGAAAATC ACCTTGACAA GGACACAGAA GGGTATGATG TGGTGCTTGA TGCAATAGAC
    ACGATGCAGC GAGTAGCCTG GCACATCAAC GACATGAAGA GGAAGCACGA ACACGCAGTC
    CGGTTACAGG AGATCCAGAG TCTGCTCACC AACTGGAAGG GGCCAGACCT GACCAGCTAT
    GGGGAACTGG TGCTCGAAGG AACCTTTCGC ATCCAGCGGG CCAAGAATGA GCGGACACTC
    TTCCTCTTTG ACAAGCTGCT GCTCATCACA AAGAAGAGAG ATGACACGTT TACATACAAA
    GCTCACATCC TTTGTGGCAA CCTCATGCTG GTGGAAGTGA TACCTAAGGA GCCGCTCAGC
    TTCAGCGTCT TCCACTATAA GAACCCAAAG CTGCAGCACA CAGTCCAGGC TAAATCCCAG
    CAGGACAAAC GCCTCTGGGT TCTGCACCTG AAGAGACTGA TCCTGGAGAA CCATGCGGCT
    AAGATTCCAG CTAAGGCCAA GCAAGCAATA CTTGAAATGG ATGCCATTCA TTATCCCGGT
    TTCTGTTACA GTCCTGAGGG AGAGACGAAA GCATTGTGTG GTTCTAAAGA TGGTTCGGCT
    CCGTATCGGC TGAGAAGAAA ATCTGAACCT TCCTCAAGAT CACATAAAGT TTTGAAGACC
    AGTGAAGCAG CACAAGACAT CCAAAAGGTT TCCAGAGAGG AAGGGTCTCC CCAGCGGACC
    TCAACCCGGC CATCTCCCGC CCAGAGGAAC AACCAGCCAA GCAGTTCTGC CATTATCAAC
    GTGCTTCGTG GGGGTGGAGC CATCAGAAAC ATCTGGACAG ACCACCAGAT CAGACAAGCC
    TTATTTCCCA GTCGTCGGTC CCCTCAGGAG AACGAGGATG ATGAAGATGA TTATCAGATG
    TTCGTGCCGT CCTTCTCCTC CTCAGATCTG AACTCTACCA GACTGTGTGA AGACAGCACT
    TCCAGTCGCC CTTGCAGTTG GCACATGGGA CAGATTGAGC CCACAGAGAC CACCAGCTCC
    AGCCACCGGG TTGTCAGGCG GGCCAGTAGC GCTGGTGAGA GCAATACATG CACTCCTGAA
    ATAAGAATTA GGGACAGTGG TGGCTCTCAG TACAGCCCCC GAAGGGAACT ACAGAGTGAC
    CCTAAAACAG AAGGGCAGGA TGGAATGACT CCCTTTGGGT CATCTATAGA GTTGACTATT
    GATGATATAG ACCATGTCTA TGATAACATA AGTTACGAGG ACTTAAAACT AATGGTTGCA
    AAACGGGAAG AAGCTGAATC CACACCCCTC AAAATAGCCA GGGACTCTGT TCGCCCCAAG
    AGCACCCCAG AGCTAGCCTT CTCAAAGAAG CAAGCTGGCC ACGAGAAGAG CTCTCTGCAC
    ACAAAAAGGG ATGGAGCTCT AACAGGTTGC CAGGCATCCA ACCAAAGCAC ACACGAACTC
    CAGATGGTTG AGGAAAACAT CTATGATACC ATAGGGCTCC CAGATGCACC CTCGCTGGAT
    TTTAACTGCA GCAGCCTGAA GCGTCCCAAG AGGAGCACCT TCTTAGGTCT GGAAGCCGAC
    TTTGCCTGCT GTGACAGTCT GAGGCCCTTC ATTTCCCAGG ATAGCCTCCA GTTCAGTGAG
    GATGAAGCTC CTTACCACCA GGGGCCTTCT GATAATGATT ATTTGAGTTT GCTCTATAAC
    TCTTTCAGCT GTAACCTATC TATAGGTGAT AAATCTATAT CTGATAAGTT GTCTGAAGAA
    GTAGATGAAA TCTGGAATGA CCTGGAAAAT TACATCAAGA AAAACGAAGT CAAAGCTAGA
    GACCGGCTCC TCGCAGCATT TCCTGTCAGC AAAGATGACG TGCCAGACAG GCTGCACTCC
    GAGAGCACAC CTGAGCTGGG CAGAGATGCG GCGCATTCTG TGTCTACCCT CTCGCTTCCT
    GAAGGTCACA CCTTCCTCAC GCCCCTGAAA GACAAGCCTG GCAGAGCTGG CCGGGCCAAC
    TGCCCTTTTG AAGAGGACCT GATTTCTAAA GAAGCCTCCT TTATGAGTCT TAACCGGCTG
    TCTTTGGCCA GTGAAACACC TCCCATGGAA AACCCCTATG ACTTGGCCAA CAGCAGCAGT
    CTGTCCCAAT CAGACTTGGA AATCCCTGAC GCCGGGATGG ATGTCAGTGA TAAGACAAGA
    AGCAGGGTTT TTATGATGGC CAGGCAGTAT AGTCAGAAGA TCAAGAAGGC AAATCAACTT
    TTAAAAGTGA GAAGCCCAGA GTTGGAACAG CCACTGGCCA GCCAGCAGCA TAAATCCACG
    CACAAAGACC TGGCAGCCAT CTTGGAAGAA AAGAAGCAAG GGGGTCCTGC CATCGGTGCC
    AGGATCGCTG AGTATTCCCA GCTGTATGAC CAGATTGTGT TCAGAGAGAC TCCTTGTAAG
    ACTCAGAAGG ATGGCTGGGC CAGCCCTCAA GAGCCCTCCA ACCTCAGATC TACATCATCT
    TCCCAGGCCC AACTTAGCAG TGAGGATTGG CTTTTGCATT CAACCTATAG TAATGGAGAG
    TTAGTGGATT TCTGTCCCCG GCCAGAGCAA GACTTGAAGT CAAAACATCC TACTTTAGAG
    ATCAGTACAA AAAGCATTCC AAGACAGCTG TCCACAGCTT GCTCTGTGCC TTCTCTTCAA
    ACCTCTGACC CTCTGCTGTG CTCCTTGCAG AGACACAGTG TGATAGTCAG CCAGCCCAAC
    AAAGATAACT CGTATCAGGG CCACCTCTAC AATTCGTTGG GTCGGAAAAG CATCAGTGCG
    AAATCTCAGC CTTATAGCAG GTCTCAGTCC TCTTCTTCAA TCTTGATAAA CAAGTCGGTG
    GACTCCATCA GCTACCCTGG TGAAATGGGA AAGAAACAGC CACTGTCTAT CCACAAAAGT
    TCGAGGTGTG AGAGTCACCA GGATTTGCTG CCAGCTATGG CTGACCCGTG TCAGCAGGGC
    CCTGAAAAAC ACTCAGACCT CACGCTCCGA GACTCACAGA AGGTTCTGGT AGTCAATAGA
    AATGTACCCT TAAATGCCCA AATAGCCACA CAGAACTATT TTTCCAATTT CAAAGAGACT
    GAAGGAGACG AAGACGACTA TGTGGAAATA AGATCTGAAG AAGACGAGTC AGAAGTAGAG
    CCATCCCATG GTCGGAGGAA GAAATCTGAC CCAAGGATTG CAGAAGCTGA CTTTTCGGAG
    GACCCCTGCA CCAACAGCAC CCCTTACTCT TTGAATAGTC CATGCACTCC AAAAAAGCCG
    ATAAGTGACA AGCTTGGGAT TTCACCCTAC CTGGCATCGT ACAGCGATTC GGACAAACTG
    AACGACTATC TCTGGAGGGG CCCGTCTCCC AACCAGCAAA ATATTGTCCA GTCGCTGAGG
    GAAAAATTTC AGTGTCTCAG TTCAAGCAGC TTTGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005615567.1
    CDS242..4420
    Translation

    Target ORF information:

    RefSeq Version XM_005615510.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris pleckstrin homology and RhoGEF domain containing G1 (PLEKHG1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005615510.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAGCTCT CGGACAGCGA CCGCCCGGTC AGCTTCGGCT CCACGTCGTC CTCGGCTTCT 
    TCCCGGGACA GCCACGGTTC CTTCGGCAGC AGAATGACCT TAGTCTCAAA CAGCCACTTG
    GGCTTGTTTC ACCAAGATAA GGAAGCCGGG GCCATAAAAC TGGAGCTGAT TCCAGCCAGG
    CCGCTTTCCA GCAGCGAGCT GCAGCGGGCC GGCCCCGCTG GGCCACAGGA CACGGGCGAC
    GGCGGCGACA GGCAGCCCCG GGCCCAGCGG AGAATGGAAG CAAACGGGGC CACCAGAGCA
    GGTGCAGAGT CGGCCACCAG CCCCAAGCTC CTGTATGTGG ATAGGGTTGT TCAGGAGATT
    CTGGAGACTG AGAGGACTTA CGTGCAAGAC TTGAAAAGCA TCGTAGAGGA TTACCTTGAC
    TGCATCAGGG ACCAAACAAA ACTTCCTCTG GGGACAGAAG AAAGATCAGC CCTCTTTGGA
    AACATTCAGG ATATCTACCA TTTCAATAGT GAACTTCTAC AAGATTTGGA AAACTGTGAA
    AATGATCCTG TGGCCATTGC AGAATGTTTT GTATCCAAGA GTGAAGAGTT CCACATTTAC
    ACGCAGTACT GCACTAACTA TCCAAGATCA GTGGCTGTGC TGACAGAGTG CATGCGGAAC
    AAGATGCTGG CCAAATTCTT CAGGGAACGT CAGGAAACTT TGAAACACTC ACTGCCTTTG
    GGGTCCTATC TCTTGAAACC AGTTCAGCGG ATTCTCAAGT ATCATCTCCT TTTGCATGAA
    ATAGAAAATC ACCTTGACAA GGACACAGAA GGGTATGATG TGGTGCTTGA TGCAATAGAC
    ACGATGCAGC GAGTAGCCTG GCACATCAAC GACATGAAGA GGAAGCACGA ACACGCAGTC
    CGGTTACAGG AGATCCAGAG TCTGCTCACC AACTGGAAGG GGCCAGACCT GACCAGCTAT
    GGGGAACTGG TGCTCGAAGG AACCTTTCGC ATCCAGCGGG CCAAGAATGA GCGGACACTC
    TTCCTCTTTG ACAAGCTGCT GCTCATCACA AAGAAGAGAG ATGACACGTT TACATACAAA
    GCTCACATCC TTTGTGGCAA CCTCATGCTG GTGGAAGTGA TACCTAAGGA GCCGCTCAGC
    TTCAGCGTCT TCCACTATAA GAACCCAAAG CTGCAGCACA CAGTCCAGGC TAAATCCCAG
    CAGGACAAAC GCCTCTGGGT TCTGCACCTG AAGAGACTGA TCCTGGAGAA CCATGCGGCT
    AAGATTCCAG CTAAGGCCAA GCAAGCAATA CTTGAAATGG ATGCCATTCA TTATCCCGGT
    TTCTGTTACA GTCCTGAGGG AGAGACGAAA GCATTGTGTG GTTCTAAAGA TGGTTCGGCT
    CCGTATCGGC TGAGAAGAAA ATCTGAACCT TCCTCAAGAT CACATAAAGT TTTGAAGACC
    AGTGAAGCAG CACAAGACAT CCAAAAGGTT TCCAGAGAGG AAGGGTCTCC CCAGCGGACC
    TCAACCCGGC CATCTCCCGC CCAGAGGAAC AACCAGCCAA GCAGTTCTGC CATTATCAAC
    GTGCTTCGTG GGGGTGGAGC CATCAGAAAC ATCTGGACAG ACCACCAGAT CAGACAAGCC
    TTATTTCCCA GTCGTCGGTC CCCTCAGGAG AACGAGGATG ATGAAGATGA TTATCAGATG
    TTCGTGCCGT CCTTCTCCTC CTCAGATCTG AACTCTACCA GACTGTGTGA AGACAGCACT
    TCCAGTCGCC CTTGCAGTTG GCACATGGGA CAGATTGAGC CCACAGAGAC CACCAGCTCC
    AGCCACCGGG TTGTCAGGCG GGCCAGTAGC GCTGGTGAGA GCAATACATG CACTCCTGAA
    ATAAGAATTA GGGACAGTGG TGGCTCTCAG TACAGCCCCC GAAGGGAACT ACAGAGTGAC
    CCTAAAACAG AAGGGCAGGA TGGAATGACT CCCTTTGGGT CATCTATAGA GTTGACTATT
    GATGATATAG ACCATGTCTA TGATAACATA AGTTACGAGG ACTTAAAACT AATGGTTGCA
    AAACGGGAAG AAGCTGAATC CACACCCCTC AAAATAGCCA GGGACTCTGT TCGCCCCAAG
    AGCACCCCAG AGCTAGCCTT CTCAAAGAAG CAAGCTGGCC ACGAGAAGAG CTCTCTGCAC
    ACAAAAAGGG ATGGAGCTCT AACAGGTTGC CAGGCATCCA ACCAAAGCAC ACACGAACTC
    CAGATGGTTG AGGAAAACAT CTATGATACC ATAGGGCTCC CAGATGCACC CTCGCTGGAT
    TTTAACTGCA GCAGCCTGAA GCGTCCCAAG AGGAGCACCT TCTTAGGTCT GGAAGCCGAC
    TTTGCCTGCT GTGACAGTCT GAGGCCCTTC ATTTCCCAGG ATAGCCTCCA GTTCAGTGAG
    GATGAAGCTC CTTACCACCA GGGGCCTTCT GATAATGATT ATTTGAGTTT GCTCTATAAC
    TCTTTCAGCT GTAACCTATC TATAGGTGAT AAATCTATAT CTGATAAGTT GTCTGAAGAA
    GTAGATGAAA TCTGGAATGA CCTGGAAAAT TACATCAAGA AAAACGAAGT CAAAGCTAGA
    GACCGGCTCC TCGCAGCATT TCCTGTCAGC AAAGATGACG TGCCAGACAG GCTGCACTCC
    GAGAGCACAC CTGAGCTGGG CAGAGATGCG GCGCATTCTG TGTCTACCCT CTCGCTTCCT
    GAAGGTCACA CCTTCCTCAC GCCCCTGAAA GACAAGCCTG GCAGAGCTGG CCGGGCCAAC
    TGCCCTTTTG AAGAGGACCT GATTTCTAAA GAAGCCTCCT TTATGAGTCT TAACCGGCTG
    TCTTTGGCCA GTGAAACACC TCCCATGGAA AACCCCTATG ACTTGGCCAA CAGCAGCAGT
    CTGTCCCAAT CAGACTTGGA AATCCCTGAC GCCGGGATGG ATGTCAGTGA TAAGACAAGA
    AGCAGGGTTT TTATGATGGC CAGGCAGTAT AGTCAGAAGA TCAAGAAGGC AAATCAACTT
    TTAAAAGTGA GAAGCCCAGA GTTGGAACAG CCACTGGCCA GCCAGCAGCA TAAATCCACG
    CACAAAGACC TGGCAGCCAT CTTGGAAGAA AAGAAGCAAG GGGGTCCTGC CATCGGTGCC
    AGGATCGCTG AGTATTCCCA GCTGTATGAC CAGATTGTGT TCAGAGAGAC TCCTTGTAAG
    ACTCAGAAGG ATGGCTGGGC CAGCCCTCAA GAGCCCTCCA ACCTCAGATC TACATCATCT
    TCCCAGGCCC AACTTAGCAG TGAGGATTGG CTTTTGCATT CAACCTATAG TAATGGAGAG
    TTAGTGGATT TCTGTCCCCG GCCAGAGCAA GACTTGAAGT CAAAACATCC TACTTTAGAG
    ATCAGTACAA AAAGCATTCC AAGACAGCTG TCCACAGCTT GCTCTGTGCC TTCTCTTCAA
    ACCTCTGACC CTCTGCTGTG CTCCTTGCAG AGACACAGTG TGATAGTCAG CCAGCCCAAC
    AAAGATAACT CGTATCAGGG CCACCTCTAC AATTCGTTGG GTCGGAAAAG CATCAGTGCG
    AAATCTCAGC CTTATAGCAG GTCTCAGTCC TCTTCTTCAA TCTTGATAAA CAAGTCGGTG
    GACTCCATCA GCTACCCTGG TGAAATGGGA AAGAAACAGC CACTGTCTAT CCACAAAAGT
    TCGAGGTGTG AGAGTCACCA GGATTTGCTG CCAGCTATGG CTGACCCGTG TCAGCAGGGC
    CCTGAAAAAC ACTCAGACCT CACGCTCCGA GACTCACAGA AGGTTCTGGT AGTCAATAGA
    AATGTACCCT TAAATGCCCA AATAGCCACA CAGAACTATT TTTCCAATTT CAAAGAGACT
    GAAGGAGACG AAGACGACTA TGTGGAAATA AGATCTGAAG AAGACGAGTC AGAAGTAGAG
    CCATCCCATG GTCGGAGGAA GAAATCTGAC CCAAGGATTG CAGAAGCTGA CTTTTCGGAG
    GACCCCTGCA CCAACAGCAC CCCTTACTCT TTGAATAGTC CATGCACTCC AAAAAAGCCG
    ATAAGTGACA AGCTTGGGAT TTCACCCTAC CTGGCATCGT ACAGCGATTC GGACAAACTG
    AACGACTATC TCTGGAGGGG CCCGTCTCCC AACCAGCAAA ATATTGTCCA GTCGCTGAGG
    GAAAAATTTC AGTGTCTCAG TTCAAGCAGC TTTGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe00948
    Clone ID Related Accession (Same CDS sequence) XM_541152.6 , XM_005615510.3 , XM_005615508.3 , XM_541152.5 , XM_005615508.2 , XM_005615509.2 , XM_005615510.2
    Accession Version XM_005615508.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4179bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product pleckstrin homology domain-containing family G member 1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005615508.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAGCTCT CGGACAGCGA CCGCCCGGTC AGCTTCGGCT CCACGTCGTC CTCGGCTTCT 
    TCCCGGGACA GCCACGGTTC CTTCGGCAGC AGAATGACCT TAGTCTCAAA CAGCCACTTG
    GGCTTGTTTC ACCAAGATAA GGAAGCCGGG GCCATAAAAC TGGAGCTGAT TCCAGCCAGG
    CCGCTTTCCA GCAGCGAGCT GCAGCGGGCC GGCCCCGCTG GGCCACAGGA CACGGGCGAC
    GGCGGCGACA GGCAGCCCCG GGCCCAGCGG AGAATGGAAG CAAACGGGGC CACCAGAGCA
    GGTGCAGAGT CGGCCACCAG CCCCAAGCTC CTGTATGTGG ATAGGGTTGT TCAGGAGATT
    CTGGAGACTG AGAGGACTTA CGTGCAAGAC TTGAAAAGCA TCGTAGAGGA TTACCTTGAC
    TGCATCAGGG ACCAAACAAA ACTTCCTCTG GGGACAGAAG AAAGATCAGC CCTCTTTGGA
    AACATTCAGG ATATCTACCA TTTCAATAGT GAACTTCTAC AAGATTTGGA AAACTGTGAA
    AATGATCCTG TGGCCATTGC AGAATGTTTT GTATCCAAGA GTGAAGAGTT CCACATTTAC
    ACGCAGTACT GCACTAACTA TCCAAGATCA GTGGCTGTGC TGACAGAGTG CATGCGGAAC
    AAGATGCTGG CCAAATTCTT CAGGGAACGT CAGGAAACTT TGAAACACTC ACTGCCTTTG
    GGGTCCTATC TCTTGAAACC AGTTCAGCGG ATTCTCAAGT ATCATCTCCT TTTGCATGAA
    ATAGAAAATC ACCTTGACAA GGACACAGAA GGGTATGATG TGGTGCTTGA TGCAATAGAC
    ACGATGCAGC GAGTAGCCTG GCACATCAAC GACATGAAGA GGAAGCACGA ACACGCAGTC
    CGGTTACAGG AGATCCAGAG TCTGCTCACC AACTGGAAGG GGCCAGACCT GACCAGCTAT
    GGGGAACTGG TGCTCGAAGG AACCTTTCGC ATCCAGCGGG CCAAGAATGA GCGGACACTC
    TTCCTCTTTG ACAAGCTGCT GCTCATCACA AAGAAGAGAG ATGACACGTT TACATACAAA
    GCTCACATCC TTTGTGGCAA CCTCATGCTG GTGGAAGTGA TACCTAAGGA GCCGCTCAGC
    TTCAGCGTCT TCCACTATAA GAACCCAAAG CTGCAGCACA CAGTCCAGGC TAAATCCCAG
    CAGGACAAAC GCCTCTGGGT TCTGCACCTG AAGAGACTGA TCCTGGAGAA CCATGCGGCT
    AAGATTCCAG CTAAGGCCAA GCAAGCAATA CTTGAAATGG ATGCCATTCA TTATCCCGGT
    TTCTGTTACA GTCCTGAGGG AGAGACGAAA GCATTGTGTG GTTCTAAAGA TGGTTCGGCT
    CCGTATCGGC TGAGAAGAAA ATCTGAACCT TCCTCAAGAT CACATAAAGT TTTGAAGACC
    AGTGAAGCAG CACAAGACAT CCAAAAGGTT TCCAGAGAGG AAGGGTCTCC CCAGCGGACC
    TCAACCCGGC CATCTCCCGC CCAGAGGAAC AACCAGCCAA GCAGTTCTGC CATTATCAAC
    GTGCTTCGTG GGGGTGGAGC CATCAGAAAC ATCTGGACAG ACCACCAGAT CAGACAAGCC
    TTATTTCCCA GTCGTCGGTC CCCTCAGGAG AACGAGGATG ATGAAGATGA TTATCAGATG
    TTCGTGCCGT CCTTCTCCTC CTCAGATCTG AACTCTACCA GACTGTGTGA AGACAGCACT
    TCCAGTCGCC CTTGCAGTTG GCACATGGGA CAGATTGAGC CCACAGAGAC CACCAGCTCC
    AGCCACCGGG TTGTCAGGCG GGCCAGTAGC GCTGGTGAGA GCAATACATG CACTCCTGAA
    ATAAGAATTA GGGACAGTGG TGGCTCTCAG TACAGCCCCC GAAGGGAACT ACAGAGTGAC
    CCTAAAACAG AAGGGCAGGA TGGAATGACT CCCTTTGGGT CATCTATAGA GTTGACTATT
    GATGATATAG ACCATGTCTA TGATAACATA AGTTACGAGG ACTTAAAACT AATGGTTGCA
    AAACGGGAAG AAGCTGAATC CACACCCCTC AAAATAGCCA GGGACTCTGT TCGCCCCAAG
    AGCACCCCAG AGCTAGCCTT CTCAAAGAAG CAAGCTGGCC ACGAGAAGAG CTCTCTGCAC
    ACAAAAAGGG ATGGAGCTCT AACAGGTTGC CAGGCATCCA ACCAAAGCAC ACACGAACTC
    CAGATGGTTG AGGAAAACAT CTATGATACC ATAGGGCTCC CAGATGCACC CTCGCTGGAT
    TTTAACTGCA GCAGCCTGAA GCGTCCCAAG AGGAGCACCT TCTTAGGTCT GGAAGCCGAC
    TTTGCCTGCT GTGACAGTCT GAGGCCCTTC ATTTCCCAGG ATAGCCTCCA GTTCAGTGAG
    GATGAAGCTC CTTACCACCA GGGGCCTTCT GATAATGATT ATTTGAGTTT GCTCTATAAC
    TCTTTCAGCT GTAACCTATC TATAGGTGAT AAATCTATAT CTGATAAGTT GTCTGAAGAA
    GTAGATGAAA TCTGGAATGA CCTGGAAAAT TACATCAAGA AAAACGAAGT CAAAGCTAGA
    GACCGGCTCC TCGCAGCATT TCCTGTCAGC AAAGATGACG TGCCAGACAG GCTGCACTCC
    GAGAGCACAC CTGAGCTGGG CAGAGATGCG GCGCATTCTG TGTCTACCCT CTCGCTTCCT
    GAAGGTCACA CCTTCCTCAC GCCCCTGAAA GACAAGCCTG GCAGAGCTGG CCGGGCCAAC
    TGCCCTTTTG AAGAGGACCT GATTTCTAAA GAAGCCTCCT TTATGAGTCT TAACCGGCTG
    TCTTTGGCCA GTGAAACACC TCCCATGGAA AACCCCTATG ACTTGGCCAA CAGCAGCAGT
    CTGTCCCAAT CAGACTTGGA AATCCCTGAC GCCGGGATGG ATGTCAGTGA TAAGACAAGA
    AGCAGGGTTT TTATGATGGC CAGGCAGTAT AGTCAGAAGA TCAAGAAGGC AAATCAACTT
    TTAAAAGTGA GAAGCCCAGA GTTGGAACAG CCACTGGCCA GCCAGCAGCA TAAATCCACG
    CACAAAGACC TGGCAGCCAT CTTGGAAGAA AAGAAGCAAG GGGGTCCTGC CATCGGTGCC
    AGGATCGCTG AGTATTCCCA GCTGTATGAC CAGATTGTGT TCAGAGAGAC TCCTTGTAAG
    ACTCAGAAGG ATGGCTGGGC CAGCCCTCAA GAGCCCTCCA ACCTCAGATC TACATCATCT
    TCCCAGGCCC AACTTAGCAG TGAGGATTGG CTTTTGCATT CAACCTATAG TAATGGAGAG
    TTAGTGGATT TCTGTCCCCG GCCAGAGCAA GACTTGAAGT CAAAACATCC TACTTTAGAG
    ATCAGTACAA AAAGCATTCC AAGACAGCTG TCCACAGCTT GCTCTGTGCC TTCTCTTCAA
    ACCTCTGACC CTCTGCTGTG CTCCTTGCAG AGACACAGTG TGATAGTCAG CCAGCCCAAC
    AAAGATAACT CGTATCAGGG CCACCTCTAC AATTCGTTGG GTCGGAAAAG CATCAGTGCG
    AAATCTCAGC CTTATAGCAG GTCTCAGTCC TCTTCTTCAA TCTTGATAAA CAAGTCGGTG
    GACTCCATCA GCTACCCTGG TGAAATGGGA AAGAAACAGC CACTGTCTAT CCACAAAAGT
    TCGAGGTGTG AGAGTCACCA GGATTTGCTG CCAGCTATGG CTGACCCGTG TCAGCAGGGC
    CCTGAAAAAC ACTCAGACCT CACGCTCCGA GACTCACAGA AGGTTCTGGT AGTCAATAGA
    AATGTACCCT TAAATGCCCA AATAGCCACA CAGAACTATT TTTCCAATTT CAAAGAGACT
    GAAGGAGACG AAGACGACTA TGTGGAAATA AGATCTGAAG AAGACGAGTC AGAAGTAGAG
    CCATCCCATG GTCGGAGGAA GAAATCTGAC CCAAGGATTG CAGAAGCTGA CTTTTCGGAG
    GACCCCTGCA CCAACAGCAC CCCTTACTCT TTGAATAGTC CATGCACTCC AAAAAAGCCG
    ATAAGTGACA AGCTTGGGAT TTCACCCTAC CTGGCATCGT ACAGCGATTC GGACAAACTG
    AACGACTATC TCTGGAGGGG CCCGTCTCCC AACCAGCAAA ATATTGTCCA GTCGCTGAGG
    GAAAAATTTC AGTGTCTCAG TTCAAGCAGC TTTGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005615565.1
    CDS285..4463
    Translation

    Target ORF information:

    RefSeq Version XM_005615508.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris pleckstrin homology and RhoGEF domain containing G1 (PLEKHG1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005615508.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAGCTCT CGGACAGCGA CCGCCCGGTC AGCTTCGGCT CCACGTCGTC CTCGGCTTCT 
    TCCCGGGACA GCCACGGTTC CTTCGGCAGC AGAATGACCT TAGTCTCAAA CAGCCACTTG
    GGCTTGTTTC ACCAAGATAA GGAAGCCGGG GCCATAAAAC TGGAGCTGAT TCCAGCCAGG
    CCGCTTTCCA GCAGCGAGCT GCAGCGGGCC GGCCCCGCTG GGCCACAGGA CACGGGCGAC
    GGCGGCGACA GGCAGCCCCG GGCCCAGCGG AGAATGGAAG CAAACGGGGC CACCAGAGCA
    GGTGCAGAGT CGGCCACCAG CCCCAAGCTC CTGTATGTGG ATAGGGTTGT TCAGGAGATT
    CTGGAGACTG AGAGGACTTA CGTGCAAGAC TTGAAAAGCA TCGTAGAGGA TTACCTTGAC
    TGCATCAGGG ACCAAACAAA ACTTCCTCTG GGGACAGAAG AAAGATCAGC CCTCTTTGGA
    AACATTCAGG ATATCTACCA TTTCAATAGT GAACTTCTAC AAGATTTGGA AAACTGTGAA
    AATGATCCTG TGGCCATTGC AGAATGTTTT GTATCCAAGA GTGAAGAGTT CCACATTTAC
    ACGCAGTACT GCACTAACTA TCCAAGATCA GTGGCTGTGC TGACAGAGTG CATGCGGAAC
    AAGATGCTGG CCAAATTCTT CAGGGAACGT CAGGAAACTT TGAAACACTC ACTGCCTTTG
    GGGTCCTATC TCTTGAAACC AGTTCAGCGG ATTCTCAAGT ATCATCTCCT TTTGCATGAA
    ATAGAAAATC ACCTTGACAA GGACACAGAA GGGTATGATG TGGTGCTTGA TGCAATAGAC
    ACGATGCAGC GAGTAGCCTG GCACATCAAC GACATGAAGA GGAAGCACGA ACACGCAGTC
    CGGTTACAGG AGATCCAGAG TCTGCTCACC AACTGGAAGG GGCCAGACCT GACCAGCTAT
    GGGGAACTGG TGCTCGAAGG AACCTTTCGC ATCCAGCGGG CCAAGAATGA GCGGACACTC
    TTCCTCTTTG ACAAGCTGCT GCTCATCACA AAGAAGAGAG ATGACACGTT TACATACAAA
    GCTCACATCC TTTGTGGCAA CCTCATGCTG GTGGAAGTGA TACCTAAGGA GCCGCTCAGC
    TTCAGCGTCT TCCACTATAA GAACCCAAAG CTGCAGCACA CAGTCCAGGC TAAATCCCAG
    CAGGACAAAC GCCTCTGGGT TCTGCACCTG AAGAGACTGA TCCTGGAGAA CCATGCGGCT
    AAGATTCCAG CTAAGGCCAA GCAAGCAATA CTTGAAATGG ATGCCATTCA TTATCCCGGT
    TTCTGTTACA GTCCTGAGGG AGAGACGAAA GCATTGTGTG GTTCTAAAGA TGGTTCGGCT
    CCGTATCGGC TGAGAAGAAA ATCTGAACCT TCCTCAAGAT CACATAAAGT TTTGAAGACC
    AGTGAAGCAG CACAAGACAT CCAAAAGGTT TCCAGAGAGG AAGGGTCTCC CCAGCGGACC
    TCAACCCGGC CATCTCCCGC CCAGAGGAAC AACCAGCCAA GCAGTTCTGC CATTATCAAC
    GTGCTTCGTG GGGGTGGAGC CATCAGAAAC ATCTGGACAG ACCACCAGAT CAGACAAGCC
    TTATTTCCCA GTCGTCGGTC CCCTCAGGAG AACGAGGATG ATGAAGATGA TTATCAGATG
    TTCGTGCCGT CCTTCTCCTC CTCAGATCTG AACTCTACCA GACTGTGTGA AGACAGCACT
    TCCAGTCGCC CTTGCAGTTG GCACATGGGA CAGATTGAGC CCACAGAGAC CACCAGCTCC
    AGCCACCGGG TTGTCAGGCG GGCCAGTAGC GCTGGTGAGA GCAATACATG CACTCCTGAA
    ATAAGAATTA GGGACAGTGG TGGCTCTCAG TACAGCCCCC GAAGGGAACT ACAGAGTGAC
    CCTAAAACAG AAGGGCAGGA TGGAATGACT CCCTTTGGGT CATCTATAGA GTTGACTATT
    GATGATATAG ACCATGTCTA TGATAACATA AGTTACGAGG ACTTAAAACT AATGGTTGCA
    AAACGGGAAG AAGCTGAATC CACACCCCTC AAAATAGCCA GGGACTCTGT TCGCCCCAAG
    AGCACCCCAG AGCTAGCCTT CTCAAAGAAG CAAGCTGGCC ACGAGAAGAG CTCTCTGCAC
    ACAAAAAGGG ATGGAGCTCT AACAGGTTGC CAGGCATCCA ACCAAAGCAC ACACGAACTC
    CAGATGGTTG AGGAAAACAT CTATGATACC ATAGGGCTCC CAGATGCACC CTCGCTGGAT
    TTTAACTGCA GCAGCCTGAA GCGTCCCAAG AGGAGCACCT TCTTAGGTCT GGAAGCCGAC
    TTTGCCTGCT GTGACAGTCT GAGGCCCTTC ATTTCCCAGG ATAGCCTCCA GTTCAGTGAG
    GATGAAGCTC CTTACCACCA GGGGCCTTCT GATAATGATT ATTTGAGTTT GCTCTATAAC
    TCTTTCAGCT GTAACCTATC TATAGGTGAT AAATCTATAT CTGATAAGTT GTCTGAAGAA
    GTAGATGAAA TCTGGAATGA CCTGGAAAAT TACATCAAGA AAAACGAAGT CAAAGCTAGA
    GACCGGCTCC TCGCAGCATT TCCTGTCAGC AAAGATGACG TGCCAGACAG GCTGCACTCC
    GAGAGCACAC CTGAGCTGGG CAGAGATGCG GCGCATTCTG TGTCTACCCT CTCGCTTCCT
    GAAGGTCACA CCTTCCTCAC GCCCCTGAAA GACAAGCCTG GCAGAGCTGG CCGGGCCAAC
    TGCCCTTTTG AAGAGGACCT GATTTCTAAA GAAGCCTCCT TTATGAGTCT TAACCGGCTG
    TCTTTGGCCA GTGAAACACC TCCCATGGAA AACCCCTATG ACTTGGCCAA CAGCAGCAGT
    CTGTCCCAAT CAGACTTGGA AATCCCTGAC GCCGGGATGG ATGTCAGTGA TAAGACAAGA
    AGCAGGGTTT TTATGATGGC CAGGCAGTAT AGTCAGAAGA TCAAGAAGGC AAATCAACTT
    TTAAAAGTGA GAAGCCCAGA GTTGGAACAG CCACTGGCCA GCCAGCAGCA TAAATCCACG
    CACAAAGACC TGGCAGCCAT CTTGGAAGAA AAGAAGCAAG GGGGTCCTGC CATCGGTGCC
    AGGATCGCTG AGTATTCCCA GCTGTATGAC CAGATTGTGT TCAGAGAGAC TCCTTGTAAG
    ACTCAGAAGG ATGGCTGGGC CAGCCCTCAA GAGCCCTCCA ACCTCAGATC TACATCATCT
    TCCCAGGCCC AACTTAGCAG TGAGGATTGG CTTTTGCATT CAACCTATAG TAATGGAGAG
    TTAGTGGATT TCTGTCCCCG GCCAGAGCAA GACTTGAAGT CAAAACATCC TACTTTAGAG
    ATCAGTACAA AAAGCATTCC AAGACAGCTG TCCACAGCTT GCTCTGTGCC TTCTCTTCAA
    ACCTCTGACC CTCTGCTGTG CTCCTTGCAG AGACACAGTG TGATAGTCAG CCAGCCCAAC
    AAAGATAACT CGTATCAGGG CCACCTCTAC AATTCGTTGG GTCGGAAAAG CATCAGTGCG
    AAATCTCAGC CTTATAGCAG GTCTCAGTCC TCTTCTTCAA TCTTGATAAA CAAGTCGGTG
    GACTCCATCA GCTACCCTGG TGAAATGGGA AAGAAACAGC CACTGTCTAT CCACAAAAGT
    TCGAGGTGTG AGAGTCACCA GGATTTGCTG CCAGCTATGG CTGACCCGTG TCAGCAGGGC
    CCTGAAAAAC ACTCAGACCT CACGCTCCGA GACTCACAGA AGGTTCTGGT AGTCAATAGA
    AATGTACCCT TAAATGCCCA AATAGCCACA CAGAACTATT TTTCCAATTT CAAAGAGACT
    GAAGGAGACG AAGACGACTA TGTGGAAATA AGATCTGAAG AAGACGAGTC AGAAGTAGAG
    CCATCCCATG GTCGGAGGAA GAAATCTGAC CCAAGGATTG CAGAAGCTGA CTTTTCGGAG
    GACCCCTGCA CCAACAGCAC CCCTTACTCT TTGAATAGTC CATGCACTCC AAAAAAGCCG
    ATAAGTGACA AGCTTGGGAT TTCACCCTAC CTGGCATCGT ACAGCGATTC GGACAAACTG
    AACGACTATC TCTGGAGGGG CCCGTCTCCC AACCAGCAAA ATATTGTCCA GTCGCTGAGG
    GAAAAATTTC AGTGTCTCAG TTCAAGCAGC TTTGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe00948
    Clone ID Related Accession (Same CDS sequence) XM_541152.6 , XM_005615510.3 , XM_005615508.3 , XM_541152.5 , XM_005615508.2 , XM_005615509.2 , XM_005615510.2
    Accession Version XM_541152.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4179bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product pleckstrin homology domain-containing family G member 1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726046.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_541152.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAGCTCT CGGACAGCGA CCGCCCGGTC AGCTTCGGCT CCACGTCGTC CTCGGCTTCT 
    TCCCGGGACA GCCACGGTTC CTTCGGCAGC AGAATGACCT TAGTCTCAAA CAGCCACTTG
    GGCTTGTTTC ACCAAGATAA GGAAGCCGGG GCCATAAAAC TGGAGCTGAT TCCAGCCAGG
    CCGCTTTCCA GCAGCGAGCT GCAGCGGGCC GGCCCCGCTG GGCCACAGGA CACGGGCGAC
    GGCGGCGACA GGCAGCCCCG GGCCCAGCGG AGAATGGAAG CAAACGGGGC CACCAGAGCA
    GGTGCAGAGT CGGCCACCAG CCCCAAGCTC CTGTATGTGG ATAGGGTTGT TCAGGAGATT
    CTGGAGACTG AGAGGACTTA CGTGCAAGAC TTGAAAAGCA TCGTAGAGGA TTACCTTGAC
    TGCATCAGGG ACCAAACAAA ACTTCCTCTG GGGACAGAAG AAAGATCAGC CCTCTTTGGA
    AACATTCAGG ATATCTACCA TTTCAATAGT GAACTTCTAC AAGATTTGGA AAACTGTGAA
    AATGATCCTG TGGCCATTGC AGAATGTTTT GTATCCAAGA GTGAAGAGTT CCACATTTAC
    ACGCAGTACT GCACTAACTA TCCAAGATCA GTGGCTGTGC TGACAGAGTG CATGCGGAAC
    AAGATGCTGG CCAAATTCTT CAGGGAACGT CAGGAAACTT TGAAACACTC ACTGCCTTTG
    GGGTCCTATC TCTTGAAACC AGTTCAGCGG ATTCTCAAGT ATCATCTCCT TTTGCATGAA
    ATAGAAAATC ACCTTGACAA GGACACAGAA GGGTATGATG TGGTGCTTGA TGCAATAGAC
    ACGATGCAGC GAGTAGCCTG GCACATCAAC GACATGAAGA GGAAGCACGA ACACGCAGTC
    CGGTTACAGG AGATCCAGAG TCTGCTCACC AACTGGAAGG GGCCAGACCT GACCAGCTAT
    GGGGAACTGG TGCTCGAAGG AACCTTTCGC ATCCAGCGGG CCAAGAATGA GCGGACACTC
    TTCCTCTTTG ACAAGCTGCT GCTCATCACA AAGAAGAGAG ATGACACGTT TACATACAAA
    GCTCACATCC TTTGTGGCAA CCTCATGCTG GTGGAAGTGA TACCTAAGGA GCCGCTCAGC
    TTCAGCGTCT TCCACTATAA GAACCCAAAG CTGCAGCACA CAGTCCAGGC TAAATCCCAG
    CAGGACAAAC GCCTCTGGGT TCTGCACCTG AAGAGACTGA TCCTGGAGAA CCATGCGGCT
    AAGATTCCAG CTAAGGCCAA GCAAGCAATA CTTGAAATGG ATGCCATTCA TTATCCCGGT
    TTCTGTTACA GTCCTGAGGG AGAGACGAAA GCATTGTGTG GTTCTAAAGA TGGTTCGGCT
    CCGTATCGGC TGAGAAGAAA ATCTGAACCT TCCTCAAGAT CACATAAAGT TTTGAAGACC
    AGTGAAGCAG CACAAGACAT CCAAAAGGTT TCCAGAGAGG AAGGGTCTCC CCAGCGGACC
    TCAACCCGGC CATCTCCCGC CCAGAGGAAC AACCAGCCAA GCAGTTCTGC CATTATCAAC
    GTGCTTCGTG GGGGTGGAGC CATCAGAAAC ATCTGGACAG ACCACCAGAT CAGACAAGCC
    TTATTTCCCA GTCGTCGGTC CCCTCAGGAG AACGAGGATG ATGAAGATGA TTATCAGATG
    TTCGTGCCGT CCTTCTCCTC CTCAGATCTG AACTCTACCA GACTGTGTGA AGACAGCACT
    TCCAGTCGCC CTTGCAGTTG GCACATGGGA CAGATTGAGC CCACAGAGAC CACCAGCTCC
    AGCCACCGGG TTGTCAGGCG GGCCAGTAGC GCTGGTGAGA GCAATACATG CACTCCTGAA
    ATAAGAATTA GGGACAGTGG TGGCTCTCAG TACAGCCCCC GAAGGGAACT ACAGAGTGAC
    CCTAAAACAG AAGGGCAGGA TGGAATGACT CCCTTTGGGT CATCTATAGA GTTGACTATT
    GATGATATAG ACCATGTCTA TGATAACATA AGTTACGAGG ACTTAAAACT AATGGTTGCA
    AAACGGGAAG AAGCTGAATC CACACCCCTC AAAATAGCCA GGGACTCTGT TCGCCCCAAG
    AGCACCCCAG AGCTAGCCTT CTCAAAGAAG CAAGCTGGCC ACGAGAAGAG CTCTCTGCAC
    ACAAAAAGGG ATGGAGCTCT AACAGGTTGC CAGGCATCCA ACCAAAGCAC ACACGAACTC
    CAGATGGTTG AGGAAAACAT CTATGATACC ATAGGGCTCC CAGATGCACC CTCGCTGGAT
    TTTAACTGCA GCAGCCTGAA GCGTCCCAAG AGGAGCACCT TCTTAGGTCT GGAAGCCGAC
    TTTGCCTGCT GTGACAGTCT GAGGCCCTTC ATTTCCCAGG ATAGCCTCCA GTTCAGTGAG
    GATGAAGCTC CTTACCACCA GGGGCCTTCT GATAATGATT ATTTGAGTTT GCTCTATAAC
    TCTTTCAGCT GTAACCTATC TATAGGTGAT AAATCTATAT CTGATAAGTT GTCTGAAGAA
    GTAGATGAAA TCTGGAATGA CCTGGAAAAT TACATCAAGA AAAACGAAGT CAAAGCTAGA
    GACCGGCTCC TCGCAGCATT TCCTGTCAGC AAAGATGACG TGCCAGACAG GCTGCACTCC
    GAGAGCACAC CTGAGCTGGG CAGAGATGCG GCGCATTCTG TGTCTACCCT CTCGCTTCCT
    GAAGGTCACA CCTTCCTCAC GCCCCTGAAA GACAAGCCTG GCAGAGCTGG CCGGGCCAAC
    TGCCCTTTTG AAGAGGACCT GATTTCTAAA GAAGCCTCCT TTATGAGTCT TAACCGGCTG
    TCTTTGGCCA GTGAAACACC TCCCATGGAA AACCCCTATG ACTTGGCCAA CAGCAGCAGT
    CTGTCCCAAT CAGACTTGGA AATCCCTGAC GCCGGGATGG ATGTCAGTGA TAAGACAAGA
    AGCAGGGTTT TTATGATGGC CAGGCAGTAT AGTCAGAAGA TCAAGAAGGC AAATCAACTT
    TTAAAAGTGA GAAGCCCAGA GTTGGAACAG CCACTGGCCA GCCAGCAGCA TAAATCCACG
    CACAAAGACC TGGCAGCCAT CTTGGAAGAA AAGAAGCAAG GGGGTCCTGC CATCGGTGCC
    AGGATCGCTG AGTATTCCCA GCTGTATGAC CAGATTGTGT TCAGAGAGAC TCCTTGTAAG
    ACTCAGAAGG ATGGCTGGGC CAGCCCTCAA GAGCCCTCCA ACCTCAGATC TACATCATCT
    TCCCAGGCCC AACTTAGCAG TGAGGATTGG CTTTTGCATT CAACCTATAG TAATGGAGAG
    TTAGTGGATT TCTGTCCCCG GCCAGAGCAA GACTTGAAGT CAAAACATCC TACTTTAGAG
    ATCAGTACAA AAAGCATTCC AAGACAGCTG TCCACAGCTT GCTCTGTGCC TTCTCTTCAA
    ACCTCTGACC CTCTGCTGTG CTCCTTGCAG AGACACAGTG TGATAGTCAG CCAGCCCAAC
    AAAGATAACT CGTATCAGGG CCACCTCTAC AATTCGTTGG GTCGGAAAAG CATCAGTGCG
    AAATCTCAGC CTTATAGCAG GTCTCAGTCC TCTTCTTCAA TCTTGATAAA CAAGTCGGTG
    GACTCCATCA GCTACCCTGG TGAAATGGGA AAGAAACAGC CACTGTCTAT CCACAAAAGT
    TCGAGGTGTG AGAGTCACCA GGATTTGCTG CCAGCTATGG CTGACCCGTG TCAGCAGGGC
    CCTGAAAAAC ACTCAGACCT CACGCTCCGA GACTCACAGA AGGTTCTGGT AGTCAATAGA
    AATGTACCCT TAAATGCCCA AATAGCCACA CAGAACTATT TTTCCAATTT CAAAGAGACT
    GAAGGAGACG AAGACGACTA TGTGGAAATA AGATCTGAAG AAGACGAGTC AGAAGTAGAG
    CCATCCCATG GTCGGAGGAA GAAATCTGAC CCAAGGATTG CAGAAGCTGA CTTTTCGGAG
    GACCCCTGCA CCAACAGCAC CCCTTACTCT TTGAATAGTC CATGCACTCC AAAAAAGCCG
    ATAAGTGACA AGCTTGGGAT TTCACCCTAC CTGGCATCGT ACAGCGATTC GGACAAACTG
    AACGACTATC TCTGGAGGGG CCCGTCTCCC AACCAGCAAA ATATTGTCCA GTCGCTGAGG
    GAAAAATTTC AGTGTCTCAG TTCAAGCAGC TTTGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_541152.3
    CDS254..4432
    Translation

    Target ORF information:

    RefSeq Version XM_541152.5
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris pleckstrin homology domain containing, family G (with RhoGef domain) member 1 (PLEKHG1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_541152.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAGCTCT CGGACAGCGA CCGCCCGGTC AGCTTCGGCT CCACGTCGTC CTCGGCTTCT 
    TCCCGGGACA GCCACGGTTC CTTCGGCAGC AGAATGACCT TAGTCTCAAA CAGCCACTTG
    GGCTTGTTTC ACCAAGATAA GGAAGCCGGG GCCATAAAAC TGGAGCTGAT TCCAGCCAGG
    CCGCTTTCCA GCAGCGAGCT GCAGCGGGCC GGCCCCGCTG GGCCACAGGA CACGGGCGAC
    GGCGGCGACA GGCAGCCCCG GGCCCAGCGG AGAATGGAAG CAAACGGGGC CACCAGAGCA
    GGTGCAGAGT CGGCCACCAG CCCCAAGCTC CTGTATGTGG ATAGGGTTGT TCAGGAGATT
    CTGGAGACTG AGAGGACTTA CGTGCAAGAC TTGAAAAGCA TCGTAGAGGA TTACCTTGAC
    TGCATCAGGG ACCAAACAAA ACTTCCTCTG GGGACAGAAG AAAGATCAGC CCTCTTTGGA
    AACATTCAGG ATATCTACCA TTTCAATAGT GAACTTCTAC AAGATTTGGA AAACTGTGAA
    AATGATCCTG TGGCCATTGC AGAATGTTTT GTATCCAAGA GTGAAGAGTT CCACATTTAC
    ACGCAGTACT GCACTAACTA TCCAAGATCA GTGGCTGTGC TGACAGAGTG CATGCGGAAC
    AAGATGCTGG CCAAATTCTT CAGGGAACGT CAGGAAACTT TGAAACACTC ACTGCCTTTG
    GGGTCCTATC TCTTGAAACC AGTTCAGCGG ATTCTCAAGT ATCATCTCCT TTTGCATGAA
    ATAGAAAATC ACCTTGACAA GGACACAGAA GGGTATGATG TGGTGCTTGA TGCAATAGAC
    ACGATGCAGC GAGTAGCCTG GCACATCAAC GACATGAAGA GGAAGCACGA ACACGCAGTC
    CGGTTACAGG AGATCCAGAG TCTGCTCACC AACTGGAAGG GGCCAGACCT GACCAGCTAT
    GGGGAACTGG TGCTCGAAGG AACCTTTCGC ATCCAGCGGG CCAAGAATGA GCGGACACTC
    TTCCTCTTTG ACAAGCTGCT GCTCATCACA AAGAAGAGAG ATGACACGTT TACATACAAA
    GCTCACATCC TTTGTGGCAA CCTCATGCTG GTGGAAGTGA TACCTAAGGA GCCGCTCAGC
    TTCAGCGTCT TCCACTATAA GAACCCAAAG CTGCAGCACA CAGTCCAGGC TAAATCCCAG
    CAGGACAAAC GCCTCTGGGT TCTGCACCTG AAGAGACTGA TCCTGGAGAA CCATGCGGCT
    AAGATTCCAG CTAAGGCCAA GCAAGCAATA CTTGAAATGG ATGCCATTCA TTATCCCGGT
    TTCTGTTACA GTCCTGAGGG AGAGACGAAA GCATTGTGTG GTTCTAAAGA TGGTTCGGCT
    CCGTATCGGC TGAGAAGAAA ATCTGAACCT TCCTCAAGAT CACATAAAGT TTTGAAGACC
    AGTGAAGCAG CACAAGACAT CCAAAAGGTT TCCAGAGAGG AAGGGTCTCC CCAGCGGACC
    TCAACCCGGC CATCTCCCGC CCAGAGGAAC AACCAGCCAA GCAGTTCTGC CATTATCAAC
    GTGCTTCGTG GGGGTGGAGC CATCAGAAAC ATCTGGACAG ACCACCAGAT CAGACAAGCC
    TTATTTCCCA GTCGTCGGTC CCCTCAGGAG AACGAGGATG ATGAAGATGA TTATCAGATG
    TTCGTGCCGT CCTTCTCCTC CTCAGATCTG AACTCTACCA GACTGTGTGA AGACAGCACT
    TCCAGTCGCC CTTGCAGTTG GCACATGGGA CAGATTGAGC CCACAGAGAC CACCAGCTCC
    AGCCACCGGG TTGTCAGGCG GGCCAGTAGC GCTGGTGAGA GCAATACATG CACTCCTGAA
    ATAAGAATTA GGGACAGTGG TGGCTCTCAG TACAGCCCCC GAAGGGAACT ACAGAGTGAC
    CCTAAAACAG AAGGGCAGGA TGGAATGACT CCCTTTGGGT CATCTATAGA GTTGACTATT
    GATGATATAG ACCATGTCTA TGATAACATA AGTTACGAGG ACTTAAAACT AATGGTTGCA
    AAACGGGAAG AAGCTGAATC CACACCCCTC AAAATAGCCA GGGACTCTGT TCGCCCCAAG
    AGCACCCCAG AGCTAGCCTT CTCAAAGAAG CAAGCTGGCC ACGAGAAGAG CTCTCTGCAC
    ACAAAAAGGG ATGGAGCTCT AACAGGTTGC CAGGCATCCA ACCAAAGCAC ACACGAACTC
    CAGATGGTTG AGGAAAACAT CTATGATACC ATAGGGCTCC CAGATGCACC CTCGCTGGAT
    TTTAACTGCA GCAGCCTGAA GCGTCCCAAG AGGAGCACCT TCTTAGGTCT GGAAGCCGAC
    TTTGCCTGCT GTGACAGTCT GAGGCCCTTC ATTTCCCAGG ATAGCCTCCA GTTCAGTGAG
    GATGAAGCTC CTTACCACCA GGGGCCTTCT GATAATGATT ATTTGAGTTT GCTCTATAAC
    TCTTTCAGCT GTAACCTATC TATAGGTGAT AAATCTATAT CTGATAAGTT GTCTGAAGAA
    GTAGATGAAA TCTGGAATGA CCTGGAAAAT TACATCAAGA AAAACGAAGT CAAAGCTAGA
    GACCGGCTCC TCGCAGCATT TCCTGTCAGC AAAGATGACG TGCCAGACAG GCTGCACTCC
    GAGAGCACAC CTGAGCTGGG CAGAGATGCG GCGCATTCTG TGTCTACCCT CTCGCTTCCT
    GAAGGTCACA CCTTCCTCAC GCCCCTGAAA GACAAGCCTG GCAGAGCTGG CCGGGCCAAC
    TGCCCTTTTG AAGAGGACCT GATTTCTAAA GAAGCCTCCT TTATGAGTCT TAACCGGCTG
    TCTTTGGCCA GTGAAACACC TCCCATGGAA AACCCCTATG ACTTGGCCAA CAGCAGCAGT
    CTGTCCCAAT CAGACTTGGA AATCCCTGAC GCCGGGATGG ATGTCAGTGA TAAGACAAGA
    AGCAGGGTTT TTATGATGGC CAGGCAGTAT AGTCAGAAGA TCAAGAAGGC AAATCAACTT
    TTAAAAGTGA GAAGCCCAGA GTTGGAACAG CCACTGGCCA GCCAGCAGCA TAAATCCACG
    CACAAAGACC TGGCAGCCAT CTTGGAAGAA AAGAAGCAAG GGGGTCCTGC CATCGGTGCC
    AGGATCGCTG AGTATTCCCA GCTGTATGAC CAGATTGTGT TCAGAGAGAC TCCTTGTAAG
    ACTCAGAAGG ATGGCTGGGC CAGCCCTCAA GAGCCCTCCA ACCTCAGATC TACATCATCT
    TCCCAGGCCC AACTTAGCAG TGAGGATTGG CTTTTGCATT CAACCTATAG TAATGGAGAG
    TTAGTGGATT TCTGTCCCCG GCCAGAGCAA GACTTGAAGT CAAAACATCC TACTTTAGAG
    ATCAGTACAA AAAGCATTCC AAGACAGCTG TCCACAGCTT GCTCTGTGCC TTCTCTTCAA
    ACCTCTGACC CTCTGCTGTG CTCCTTGCAG AGACACAGTG TGATAGTCAG CCAGCCCAAC
    AAAGATAACT CGTATCAGGG CCACCTCTAC AATTCGTTGG GTCGGAAAAG CATCAGTGCG
    AAATCTCAGC CTTATAGCAG GTCTCAGTCC TCTTCTTCAA TCTTGATAAA CAAGTCGGTG
    GACTCCATCA GCTACCCTGG TGAAATGGGA AAGAAACAGC CACTGTCTAT CCACAAAAGT
    TCGAGGTGTG AGAGTCACCA GGATTTGCTG CCAGCTATGG CTGACCCGTG TCAGCAGGGC
    CCTGAAAAAC ACTCAGACCT CACGCTCCGA GACTCACAGA AGGTTCTGGT AGTCAATAGA
    AATGTACCCT TAAATGCCCA AATAGCCACA CAGAACTATT TTTCCAATTT CAAAGAGACT
    GAAGGAGACG AAGACGACTA TGTGGAAATA AGATCTGAAG AAGACGAGTC AGAAGTAGAG
    CCATCCCATG GTCGGAGGAA GAAATCTGAC CCAAGGATTG CAGAAGCTGA CTTTTCGGAG
    GACCCCTGCA CCAACAGCAC CCCTTACTCT TTGAATAGTC CATGCACTCC AAAAAAGCCG
    ATAAGTGACA AGCTTGGGAT TTCACCCTAC CTGGCATCGT ACAGCGATTC GGACAAACTG
    AACGACTATC TCTGGAGGGG CCCGTCTCCC AACCAGCAAA ATATTGTCCA GTCGCTGAGG
    GAAAAATTTC AGTGTCTCAG TTCAAGCAGC TTTGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe00948
    Clone ID Related Accession (Same CDS sequence) XM_541152.6 , XM_005615510.3 , XM_005615508.3 , XM_541152.5 , XM_005615508.2 , XM_005615509.2 , XM_005615510.2
    Accession Version XM_005615508.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4179bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product pleckstrin homology domain-containing family G member 1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726046.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005615508.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAGCTCT CGGACAGCGA CCGCCCGGTC AGCTTCGGCT CCACGTCGTC CTCGGCTTCT 
    TCCCGGGACA GCCACGGTTC CTTCGGCAGC AGAATGACCT TAGTCTCAAA CAGCCACTTG
    GGCTTGTTTC ACCAAGATAA GGAAGCCGGG GCCATAAAAC TGGAGCTGAT TCCAGCCAGG
    CCGCTTTCCA GCAGCGAGCT GCAGCGGGCC GGCCCCGCTG GGCCACAGGA CACGGGCGAC
    GGCGGCGACA GGCAGCCCCG GGCCCAGCGG AGAATGGAAG CAAACGGGGC CACCAGAGCA
    GGTGCAGAGT CGGCCACCAG CCCCAAGCTC CTGTATGTGG ATAGGGTTGT TCAGGAGATT
    CTGGAGACTG AGAGGACTTA CGTGCAAGAC TTGAAAAGCA TCGTAGAGGA TTACCTTGAC
    TGCATCAGGG ACCAAACAAA ACTTCCTCTG GGGACAGAAG AAAGATCAGC CCTCTTTGGA
    AACATTCAGG ATATCTACCA TTTCAATAGT GAACTTCTAC AAGATTTGGA AAACTGTGAA
    AATGATCCTG TGGCCATTGC AGAATGTTTT GTATCCAAGA GTGAAGAGTT CCACATTTAC
    ACGCAGTACT GCACTAACTA TCCAAGATCA GTGGCTGTGC TGACAGAGTG CATGCGGAAC
    AAGATGCTGG CCAAATTCTT CAGGGAACGT CAGGAAACTT TGAAACACTC ACTGCCTTTG
    GGGTCCTATC TCTTGAAACC AGTTCAGCGG ATTCTCAAGT ATCATCTCCT TTTGCATGAA
    ATAGAAAATC ACCTTGACAA GGACACAGAA GGGTATGATG TGGTGCTTGA TGCAATAGAC
    ACGATGCAGC GAGTAGCCTG GCACATCAAC GACATGAAGA GGAAGCACGA ACACGCAGTC
    CGGTTACAGG AGATCCAGAG TCTGCTCACC AACTGGAAGG GGCCAGACCT GACCAGCTAT
    GGGGAACTGG TGCTCGAAGG AACCTTTCGC ATCCAGCGGG CCAAGAATGA GCGGACACTC
    TTCCTCTTTG ACAAGCTGCT GCTCATCACA AAGAAGAGAG ATGACACGTT TACATACAAA
    GCTCACATCC TTTGTGGCAA CCTCATGCTG GTGGAAGTGA TACCTAAGGA GCCGCTCAGC
    TTCAGCGTCT TCCACTATAA GAACCCAAAG CTGCAGCACA CAGTCCAGGC TAAATCCCAG
    CAGGACAAAC GCCTCTGGGT TCTGCACCTG AAGAGACTGA TCCTGGAGAA CCATGCGGCT
    AAGATTCCAG CTAAGGCCAA GCAAGCAATA CTTGAAATGG ATGCCATTCA TTATCCCGGT
    TTCTGTTACA GTCCTGAGGG AGAGACGAAA GCATTGTGTG GTTCTAAAGA TGGTTCGGCT
    CCGTATCGGC TGAGAAGAAA ATCTGAACCT TCCTCAAGAT CACATAAAGT TTTGAAGACC
    AGTGAAGCAG CACAAGACAT CCAAAAGGTT TCCAGAGAGG AAGGGTCTCC CCAGCGGACC
    TCAACCCGGC CATCTCCCGC CCAGAGGAAC AACCAGCCAA GCAGTTCTGC CATTATCAAC
    GTGCTTCGTG GGGGTGGAGC CATCAGAAAC ATCTGGACAG ACCACCAGAT CAGACAAGCC
    TTATTTCCCA GTCGTCGGTC CCCTCAGGAG AACGAGGATG ATGAAGATGA TTATCAGATG
    TTCGTGCCGT CCTTCTCCTC CTCAGATCTG AACTCTACCA GACTGTGTGA AGACAGCACT
    TCCAGTCGCC CTTGCAGTTG GCACATGGGA CAGATTGAGC CCACAGAGAC CACCAGCTCC
    AGCCACCGGG TTGTCAGGCG GGCCAGTAGC GCTGGTGAGA GCAATACATG CACTCCTGAA
    ATAAGAATTA GGGACAGTGG TGGCTCTCAG TACAGCCCCC GAAGGGAACT ACAGAGTGAC
    CCTAAAACAG AAGGGCAGGA TGGAATGACT CCCTTTGGGT CATCTATAGA GTTGACTATT
    GATGATATAG ACCATGTCTA TGATAACATA AGTTACGAGG ACTTAAAACT AATGGTTGCA
    AAACGGGAAG AAGCTGAATC CACACCCCTC AAAATAGCCA GGGACTCTGT TCGCCCCAAG
    AGCACCCCAG AGCTAGCCTT CTCAAAGAAG CAAGCTGGCC ACGAGAAGAG CTCTCTGCAC
    ACAAAAAGGG ATGGAGCTCT AACAGGTTGC CAGGCATCCA ACCAAAGCAC ACACGAACTC
    CAGATGGTTG AGGAAAACAT CTATGATACC ATAGGGCTCC CAGATGCACC CTCGCTGGAT
    TTTAACTGCA GCAGCCTGAA GCGTCCCAAG AGGAGCACCT TCTTAGGTCT GGAAGCCGAC
    TTTGCCTGCT GTGACAGTCT GAGGCCCTTC ATTTCCCAGG ATAGCCTCCA GTTCAGTGAG
    GATGAAGCTC CTTACCACCA GGGGCCTTCT GATAATGATT ATTTGAGTTT GCTCTATAAC
    TCTTTCAGCT GTAACCTATC TATAGGTGAT AAATCTATAT CTGATAAGTT GTCTGAAGAA
    GTAGATGAAA TCTGGAATGA CCTGGAAAAT TACATCAAGA AAAACGAAGT CAAAGCTAGA
    GACCGGCTCC TCGCAGCATT TCCTGTCAGC AAAGATGACG TGCCAGACAG GCTGCACTCC
    GAGAGCACAC CTGAGCTGGG CAGAGATGCG GCGCATTCTG TGTCTACCCT CTCGCTTCCT
    GAAGGTCACA CCTTCCTCAC GCCCCTGAAA GACAAGCCTG GCAGAGCTGG CCGGGCCAAC
    TGCCCTTTTG AAGAGGACCT GATTTCTAAA GAAGCCTCCT TTATGAGTCT TAACCGGCTG
    TCTTTGGCCA GTGAAACACC TCCCATGGAA AACCCCTATG ACTTGGCCAA CAGCAGCAGT
    CTGTCCCAAT CAGACTTGGA AATCCCTGAC GCCGGGATGG ATGTCAGTGA TAAGACAAGA
    AGCAGGGTTT TTATGATGGC CAGGCAGTAT AGTCAGAAGA TCAAGAAGGC AAATCAACTT
    TTAAAAGTGA GAAGCCCAGA GTTGGAACAG CCACTGGCCA GCCAGCAGCA TAAATCCACG
    CACAAAGACC TGGCAGCCAT CTTGGAAGAA AAGAAGCAAG GGGGTCCTGC CATCGGTGCC
    AGGATCGCTG AGTATTCCCA GCTGTATGAC CAGATTGTGT TCAGAGAGAC TCCTTGTAAG
    ACTCAGAAGG ATGGCTGGGC CAGCCCTCAA GAGCCCTCCA ACCTCAGATC TACATCATCT
    TCCCAGGCCC AACTTAGCAG TGAGGATTGG CTTTTGCATT CAACCTATAG TAATGGAGAG
    TTAGTGGATT TCTGTCCCCG GCCAGAGCAA GACTTGAAGT CAAAACATCC TACTTTAGAG
    ATCAGTACAA AAAGCATTCC AAGACAGCTG TCCACAGCTT GCTCTGTGCC TTCTCTTCAA
    ACCTCTGACC CTCTGCTGTG CTCCTTGCAG AGACACAGTG TGATAGTCAG CCAGCCCAAC
    AAAGATAACT CGTATCAGGG CCACCTCTAC AATTCGTTGG GTCGGAAAAG CATCAGTGCG
    AAATCTCAGC CTTATAGCAG GTCTCAGTCC TCTTCTTCAA TCTTGATAAA CAAGTCGGTG
    GACTCCATCA GCTACCCTGG TGAAATGGGA AAGAAACAGC CACTGTCTAT CCACAAAAGT
    TCGAGGTGTG AGAGTCACCA GGATTTGCTG CCAGCTATGG CTGACCCGTG TCAGCAGGGC
    CCTGAAAAAC ACTCAGACCT CACGCTCCGA GACTCACAGA AGGTTCTGGT AGTCAATAGA
    AATGTACCCT TAAATGCCCA AATAGCCACA CAGAACTATT TTTCCAATTT CAAAGAGACT
    GAAGGAGACG AAGACGACTA TGTGGAAATA AGATCTGAAG AAGACGAGTC AGAAGTAGAG
    CCATCCCATG GTCGGAGGAA GAAATCTGAC CCAAGGATTG CAGAAGCTGA CTTTTCGGAG
    GACCCCTGCA CCAACAGCAC CCCTTACTCT TTGAATAGTC CATGCACTCC AAAAAAGCCG
    ATAAGTGACA AGCTTGGGAT TTCACCCTAC CTGGCATCGT ACAGCGATTC GGACAAACTG
    AACGACTATC TCTGGAGGGG CCCGTCTCCC AACCAGCAAA ATATTGTCCA GTCGCTGAGG
    GAAAAATTTC AGTGTCTCAG TTCAAGCAGC TTTGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005615565.1
    CDS312..4490
    Translation

    Target ORF information:

    RefSeq Version XM_005615508.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris pleckstrin homology domain containing, family G (with RhoGef domain) member 1 (PLEKHG1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005615508.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAGCTCT CGGACAGCGA CCGCCCGGTC AGCTTCGGCT CCACGTCGTC CTCGGCTTCT 
    TCCCGGGACA GCCACGGTTC CTTCGGCAGC AGAATGACCT TAGTCTCAAA CAGCCACTTG
    GGCTTGTTTC ACCAAGATAA GGAAGCCGGG GCCATAAAAC TGGAGCTGAT TCCAGCCAGG
    CCGCTTTCCA GCAGCGAGCT GCAGCGGGCC GGCCCCGCTG GGCCACAGGA CACGGGCGAC
    GGCGGCGACA GGCAGCCCCG GGCCCAGCGG AGAATGGAAG CAAACGGGGC CACCAGAGCA
    GGTGCAGAGT CGGCCACCAG CCCCAAGCTC CTGTATGTGG ATAGGGTTGT TCAGGAGATT
    CTGGAGACTG AGAGGACTTA CGTGCAAGAC TTGAAAAGCA TCGTAGAGGA TTACCTTGAC
    TGCATCAGGG ACCAAACAAA ACTTCCTCTG GGGACAGAAG AAAGATCAGC CCTCTTTGGA
    AACATTCAGG ATATCTACCA TTTCAATAGT GAACTTCTAC AAGATTTGGA AAACTGTGAA
    AATGATCCTG TGGCCATTGC AGAATGTTTT GTATCCAAGA GTGAAGAGTT CCACATTTAC
    ACGCAGTACT GCACTAACTA TCCAAGATCA GTGGCTGTGC TGACAGAGTG CATGCGGAAC
    AAGATGCTGG CCAAATTCTT CAGGGAACGT CAGGAAACTT TGAAACACTC ACTGCCTTTG
    GGGTCCTATC TCTTGAAACC AGTTCAGCGG ATTCTCAAGT ATCATCTCCT TTTGCATGAA
    ATAGAAAATC ACCTTGACAA GGACACAGAA GGGTATGATG TGGTGCTTGA TGCAATAGAC
    ACGATGCAGC GAGTAGCCTG GCACATCAAC GACATGAAGA GGAAGCACGA ACACGCAGTC
    CGGTTACAGG AGATCCAGAG TCTGCTCACC AACTGGAAGG GGCCAGACCT GACCAGCTAT
    GGGGAACTGG TGCTCGAAGG AACCTTTCGC ATCCAGCGGG CCAAGAATGA GCGGACACTC
    TTCCTCTTTG ACAAGCTGCT GCTCATCACA AAGAAGAGAG ATGACACGTT TACATACAAA
    GCTCACATCC TTTGTGGCAA CCTCATGCTG GTGGAAGTGA TACCTAAGGA GCCGCTCAGC
    TTCAGCGTCT TCCACTATAA GAACCCAAAG CTGCAGCACA CAGTCCAGGC TAAATCCCAG
    CAGGACAAAC GCCTCTGGGT TCTGCACCTG AAGAGACTGA TCCTGGAGAA CCATGCGGCT
    AAGATTCCAG CTAAGGCCAA GCAAGCAATA CTTGAAATGG ATGCCATTCA TTATCCCGGT
    TTCTGTTACA GTCCTGAGGG AGAGACGAAA GCATTGTGTG GTTCTAAAGA TGGTTCGGCT
    CCGTATCGGC TGAGAAGAAA ATCTGAACCT TCCTCAAGAT CACATAAAGT TTTGAAGACC
    AGTGAAGCAG CACAAGACAT CCAAAAGGTT TCCAGAGAGG AAGGGTCTCC CCAGCGGACC
    TCAACCCGGC CATCTCCCGC CCAGAGGAAC AACCAGCCAA GCAGTTCTGC CATTATCAAC
    GTGCTTCGTG GGGGTGGAGC CATCAGAAAC ATCTGGACAG ACCACCAGAT CAGACAAGCC
    TTATTTCCCA GTCGTCGGTC CCCTCAGGAG AACGAGGATG ATGAAGATGA TTATCAGATG
    TTCGTGCCGT CCTTCTCCTC CTCAGATCTG AACTCTACCA GACTGTGTGA AGACAGCACT
    TCCAGTCGCC CTTGCAGTTG GCACATGGGA CAGATTGAGC CCACAGAGAC CACCAGCTCC
    AGCCACCGGG TTGTCAGGCG GGCCAGTAGC GCTGGTGAGA GCAATACATG CACTCCTGAA
    ATAAGAATTA GGGACAGTGG TGGCTCTCAG TACAGCCCCC GAAGGGAACT ACAGAGTGAC
    CCTAAAACAG AAGGGCAGGA TGGAATGACT CCCTTTGGGT CATCTATAGA GTTGACTATT
    GATGATATAG ACCATGTCTA TGATAACATA AGTTACGAGG ACTTAAAACT AATGGTTGCA
    AAACGGGAAG AAGCTGAATC CACACCCCTC AAAATAGCCA GGGACTCTGT TCGCCCCAAG
    AGCACCCCAG AGCTAGCCTT CTCAAAGAAG CAAGCTGGCC ACGAGAAGAG CTCTCTGCAC
    ACAAAAAGGG ATGGAGCTCT AACAGGTTGC CAGGCATCCA ACCAAAGCAC ACACGAACTC
    CAGATGGTTG AGGAAAACAT CTATGATACC ATAGGGCTCC CAGATGCACC CTCGCTGGAT
    TTTAACTGCA GCAGCCTGAA GCGTCCCAAG AGGAGCACCT TCTTAGGTCT GGAAGCCGAC
    TTTGCCTGCT GTGACAGTCT GAGGCCCTTC ATTTCCCAGG ATAGCCTCCA GTTCAGTGAG
    GATGAAGCTC CTTACCACCA GGGGCCTTCT GATAATGATT ATTTGAGTTT GCTCTATAAC
    TCTTTCAGCT GTAACCTATC TATAGGTGAT AAATCTATAT CTGATAAGTT GTCTGAAGAA
    GTAGATGAAA TCTGGAATGA CCTGGAAAAT TACATCAAGA AAAACGAAGT CAAAGCTAGA
    GACCGGCTCC TCGCAGCATT TCCTGTCAGC AAAGATGACG TGCCAGACAG GCTGCACTCC
    GAGAGCACAC CTGAGCTGGG CAGAGATGCG GCGCATTCTG TGTCTACCCT CTCGCTTCCT
    GAAGGTCACA CCTTCCTCAC GCCCCTGAAA GACAAGCCTG GCAGAGCTGG CCGGGCCAAC
    TGCCCTTTTG AAGAGGACCT GATTTCTAAA GAAGCCTCCT TTATGAGTCT TAACCGGCTG
    TCTTTGGCCA GTGAAACACC TCCCATGGAA AACCCCTATG ACTTGGCCAA CAGCAGCAGT
    CTGTCCCAAT CAGACTTGGA AATCCCTGAC GCCGGGATGG ATGTCAGTGA TAAGACAAGA
    AGCAGGGTTT TTATGATGGC CAGGCAGTAT AGTCAGAAGA TCAAGAAGGC AAATCAACTT
    TTAAAAGTGA GAAGCCCAGA GTTGGAACAG CCACTGGCCA GCCAGCAGCA TAAATCCACG
    CACAAAGACC TGGCAGCCAT CTTGGAAGAA AAGAAGCAAG GGGGTCCTGC CATCGGTGCC
    AGGATCGCTG AGTATTCCCA GCTGTATGAC CAGATTGTGT TCAGAGAGAC TCCTTGTAAG
    ACTCAGAAGG ATGGCTGGGC CAGCCCTCAA GAGCCCTCCA ACCTCAGATC TACATCATCT
    TCCCAGGCCC AACTTAGCAG TGAGGATTGG CTTTTGCATT CAACCTATAG TAATGGAGAG
    TTAGTGGATT TCTGTCCCCG GCCAGAGCAA GACTTGAAGT CAAAACATCC TACTTTAGAG
    ATCAGTACAA AAAGCATTCC AAGACAGCTG TCCACAGCTT GCTCTGTGCC TTCTCTTCAA
    ACCTCTGACC CTCTGCTGTG CTCCTTGCAG AGACACAGTG TGATAGTCAG CCAGCCCAAC
    AAAGATAACT CGTATCAGGG CCACCTCTAC AATTCGTTGG GTCGGAAAAG CATCAGTGCG
    AAATCTCAGC CTTATAGCAG GTCTCAGTCC TCTTCTTCAA TCTTGATAAA CAAGTCGGTG
    GACTCCATCA GCTACCCTGG TGAAATGGGA AAGAAACAGC CACTGTCTAT CCACAAAAGT
    TCGAGGTGTG AGAGTCACCA GGATTTGCTG CCAGCTATGG CTGACCCGTG TCAGCAGGGC
    CCTGAAAAAC ACTCAGACCT CACGCTCCGA GACTCACAGA AGGTTCTGGT AGTCAATAGA
    AATGTACCCT TAAATGCCCA AATAGCCACA CAGAACTATT TTTCCAATTT CAAAGAGACT
    GAAGGAGACG AAGACGACTA TGTGGAAATA AGATCTGAAG AAGACGAGTC AGAAGTAGAG
    CCATCCCATG GTCGGAGGAA GAAATCTGAC CCAAGGATTG CAGAAGCTGA CTTTTCGGAG
    GACCCCTGCA CCAACAGCAC CCCTTACTCT TTGAATAGTC CATGCACTCC AAAAAAGCCG
    ATAAGTGACA AGCTTGGGAT TTCACCCTAC CTGGCATCGT ACAGCGATTC GGACAAACTG
    AACGACTATC TCTGGAGGGG CCCGTCTCCC AACCAGCAAA ATATTGTCCA GTCGCTGAGG
    GAAAAATTTC AGTGTCTCAG TTCAAGCAGC TTTGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe00948
    Clone ID Related Accession (Same CDS sequence) XM_541152.6 , XM_005615510.3 , XM_005615508.3 , XM_541152.5 , XM_005615508.2 , XM_005615509.2 , XM_005615510.2
    Accession Version XM_005615509.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4179bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product pleckstrin homology domain-containing family G member 1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726046.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005615509.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAGCTCT CGGACAGCGA CCGCCCGGTC AGCTTCGGCT CCACGTCGTC CTCGGCTTCT 
    TCCCGGGACA GCCACGGTTC CTTCGGCAGC AGAATGACCT TAGTCTCAAA CAGCCACTTG
    GGCTTGTTTC ACCAAGATAA GGAAGCCGGG GCCATAAAAC TGGAGCTGAT TCCAGCCAGG
    CCGCTTTCCA GCAGCGAGCT GCAGCGGGCC GGCCCCGCTG GGCCACAGGA CACGGGCGAC
    GGCGGCGACA GGCAGCCCCG GGCCCAGCGG AGAATGGAAG CAAACGGGGC CACCAGAGCA
    GGTGCAGAGT CGGCCACCAG CCCCAAGCTC CTGTATGTGG ATAGGGTTGT TCAGGAGATT
    CTGGAGACTG AGAGGACTTA CGTGCAAGAC TTGAAAAGCA TCGTAGAGGA TTACCTTGAC
    TGCATCAGGG ACCAAACAAA ACTTCCTCTG GGGACAGAAG AAAGATCAGC CCTCTTTGGA
    AACATTCAGG ATATCTACCA TTTCAATAGT GAACTTCTAC AAGATTTGGA AAACTGTGAA
    AATGATCCTG TGGCCATTGC AGAATGTTTT GTATCCAAGA GTGAAGAGTT CCACATTTAC
    ACGCAGTACT GCACTAACTA TCCAAGATCA GTGGCTGTGC TGACAGAGTG CATGCGGAAC
    AAGATGCTGG CCAAATTCTT CAGGGAACGT CAGGAAACTT TGAAACACTC ACTGCCTTTG
    GGGTCCTATC TCTTGAAACC AGTTCAGCGG ATTCTCAAGT ATCATCTCCT TTTGCATGAA
    ATAGAAAATC ACCTTGACAA GGACACAGAA GGGTATGATG TGGTGCTTGA TGCAATAGAC
    ACGATGCAGC GAGTAGCCTG GCACATCAAC GACATGAAGA GGAAGCACGA ACACGCAGTC
    CGGTTACAGG AGATCCAGAG TCTGCTCACC AACTGGAAGG GGCCAGACCT GACCAGCTAT
    GGGGAACTGG TGCTCGAAGG AACCTTTCGC ATCCAGCGGG CCAAGAATGA GCGGACACTC
    TTCCTCTTTG ACAAGCTGCT GCTCATCACA AAGAAGAGAG ATGACACGTT TACATACAAA
    GCTCACATCC TTTGTGGCAA CCTCATGCTG GTGGAAGTGA TACCTAAGGA GCCGCTCAGC
    TTCAGCGTCT TCCACTATAA GAACCCAAAG CTGCAGCACA CAGTCCAGGC TAAATCCCAG
    CAGGACAAAC GCCTCTGGGT TCTGCACCTG AAGAGACTGA TCCTGGAGAA CCATGCGGCT
    AAGATTCCAG CTAAGGCCAA GCAAGCAATA CTTGAAATGG ATGCCATTCA TTATCCCGGT
    TTCTGTTACA GTCCTGAGGG AGAGACGAAA GCATTGTGTG GTTCTAAAGA TGGTTCGGCT
    CCGTATCGGC TGAGAAGAAA ATCTGAACCT TCCTCAAGAT CACATAAAGT TTTGAAGACC
    AGTGAAGCAG CACAAGACAT CCAAAAGGTT TCCAGAGAGG AAGGGTCTCC CCAGCGGACC
    TCAACCCGGC CATCTCCCGC CCAGAGGAAC AACCAGCCAA GCAGTTCTGC CATTATCAAC
    GTGCTTCGTG GGGGTGGAGC CATCAGAAAC ATCTGGACAG ACCACCAGAT CAGACAAGCC
    TTATTTCCCA GTCGTCGGTC CCCTCAGGAG AACGAGGATG ATGAAGATGA TTATCAGATG
    TTCGTGCCGT CCTTCTCCTC CTCAGATCTG AACTCTACCA GACTGTGTGA AGACAGCACT
    TCCAGTCGCC CTTGCAGTTG GCACATGGGA CAGATTGAGC CCACAGAGAC CACCAGCTCC
    AGCCACCGGG TTGTCAGGCG GGCCAGTAGC GCTGGTGAGA GCAATACATG CACTCCTGAA
    ATAAGAATTA GGGACAGTGG TGGCTCTCAG TACAGCCCCC GAAGGGAACT ACAGAGTGAC
    CCTAAAACAG AAGGGCAGGA TGGAATGACT CCCTTTGGGT CATCTATAGA GTTGACTATT
    GATGATATAG ACCATGTCTA TGATAACATA AGTTACGAGG ACTTAAAACT AATGGTTGCA
    AAACGGGAAG AAGCTGAATC CACACCCCTC AAAATAGCCA GGGACTCTGT TCGCCCCAAG
    AGCACCCCAG AGCTAGCCTT CTCAAAGAAG CAAGCTGGCC ACGAGAAGAG CTCTCTGCAC
    ACAAAAAGGG ATGGAGCTCT AACAGGTTGC CAGGCATCCA ACCAAAGCAC ACACGAACTC
    CAGATGGTTG AGGAAAACAT CTATGATACC ATAGGGCTCC CAGATGCACC CTCGCTGGAT
    TTTAACTGCA GCAGCCTGAA GCGTCCCAAG AGGAGCACCT TCTTAGGTCT GGAAGCCGAC
    TTTGCCTGCT GTGACAGTCT GAGGCCCTTC ATTTCCCAGG ATAGCCTCCA GTTCAGTGAG
    GATGAAGCTC CTTACCACCA GGGGCCTTCT GATAATGATT ATTTGAGTTT GCTCTATAAC
    TCTTTCAGCT GTAACCTATC TATAGGTGAT AAATCTATAT CTGATAAGTT GTCTGAAGAA
    GTAGATGAAA TCTGGAATGA CCTGGAAAAT TACATCAAGA AAAACGAAGT CAAAGCTAGA
    GACCGGCTCC TCGCAGCATT TCCTGTCAGC AAAGATGACG TGCCAGACAG GCTGCACTCC
    GAGAGCACAC CTGAGCTGGG CAGAGATGCG GCGCATTCTG TGTCTACCCT CTCGCTTCCT
    GAAGGTCACA CCTTCCTCAC GCCCCTGAAA GACAAGCCTG GCAGAGCTGG CCGGGCCAAC
    TGCCCTTTTG AAGAGGACCT GATTTCTAAA GAAGCCTCCT TTATGAGTCT TAACCGGCTG
    TCTTTGGCCA GTGAAACACC TCCCATGGAA AACCCCTATG ACTTGGCCAA CAGCAGCAGT
    CTGTCCCAAT CAGACTTGGA AATCCCTGAC GCCGGGATGG ATGTCAGTGA TAAGACAAGA
    AGCAGGGTTT TTATGATGGC CAGGCAGTAT AGTCAGAAGA TCAAGAAGGC AAATCAACTT
    TTAAAAGTGA GAAGCCCAGA GTTGGAACAG CCACTGGCCA GCCAGCAGCA TAAATCCACG
    CACAAAGACC TGGCAGCCAT CTTGGAAGAA AAGAAGCAAG GGGGTCCTGC CATCGGTGCC
    AGGATCGCTG AGTATTCCCA GCTGTATGAC CAGATTGTGT TCAGAGAGAC TCCTTGTAAG
    ACTCAGAAGG ATGGCTGGGC CAGCCCTCAA GAGCCCTCCA ACCTCAGATC TACATCATCT
    TCCCAGGCCC AACTTAGCAG TGAGGATTGG CTTTTGCATT CAACCTATAG TAATGGAGAG
    TTAGTGGATT TCTGTCCCCG GCCAGAGCAA GACTTGAAGT CAAAACATCC TACTTTAGAG
    ATCAGTACAA AAAGCATTCC AAGACAGCTG TCCACAGCTT GCTCTGTGCC TTCTCTTCAA
    ACCTCTGACC CTCTGCTGTG CTCCTTGCAG AGACACAGTG TGATAGTCAG CCAGCCCAAC
    AAAGATAACT CGTATCAGGG CCACCTCTAC AATTCGTTGG GTCGGAAAAG CATCAGTGCG
    AAATCTCAGC CTTATAGCAG GTCTCAGTCC TCTTCTTCAA TCTTGATAAA CAAGTCGGTG
    GACTCCATCA GCTACCCTGG TGAAATGGGA AAGAAACAGC CACTGTCTAT CCACAAAAGT
    TCGAGGTGTG AGAGTCACCA GGATTTGCTG CCAGCTATGG CTGACCCGTG TCAGCAGGGC
    CCTGAAAAAC ACTCAGACCT CACGCTCCGA GACTCACAGA AGGTTCTGGT AGTCAATAGA
    AATGTACCCT TAAATGCCCA AATAGCCACA CAGAACTATT TTTCCAATTT CAAAGAGACT
    GAAGGAGACG AAGACGACTA TGTGGAAATA AGATCTGAAG AAGACGAGTC AGAAGTAGAG
    CCATCCCATG GTCGGAGGAA GAAATCTGAC CCAAGGATTG CAGAAGCTGA CTTTTCGGAG
    GACCCCTGCA CCAACAGCAC CCCTTACTCT TTGAATAGTC CATGCACTCC AAAAAAGCCG
    ATAAGTGACA AGCTTGGGAT TTCACCCTAC CTGGCATCGT ACAGCGATTC GGACAAACTG
    AACGACTATC TCTGGAGGGG CCCGTCTCCC AACCAGCAAA ATATTGTCCA GTCGCTGAGG
    GAAAAATTTC AGTGTCTCAG TTCAAGCAGC TTTGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005615566.1
    CDS335..4513
    Translation

    Target ORF information:

    RefSeq Version XM_005615509.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris pleckstrin homology domain containing, family G (with RhoGef domain) member 1 (PLEKHG1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005615509.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAGCTCT CGGACAGCGA CCGCCCGGTC AGCTTCGGCT CCACGTCGTC CTCGGCTTCT 
    TCCCGGGACA GCCACGGTTC CTTCGGCAGC AGAATGACCT TAGTCTCAAA CAGCCACTTG
    GGCTTGTTTC ACCAAGATAA GGAAGCCGGG GCCATAAAAC TGGAGCTGAT TCCAGCCAGG
    CCGCTTTCCA GCAGCGAGCT GCAGCGGGCC GGCCCCGCTG GGCCACAGGA CACGGGCGAC
    GGCGGCGACA GGCAGCCCCG GGCCCAGCGG AGAATGGAAG CAAACGGGGC CACCAGAGCA
    GGTGCAGAGT CGGCCACCAG CCCCAAGCTC CTGTATGTGG ATAGGGTTGT TCAGGAGATT
    CTGGAGACTG AGAGGACTTA CGTGCAAGAC TTGAAAAGCA TCGTAGAGGA TTACCTTGAC
    TGCATCAGGG ACCAAACAAA ACTTCCTCTG GGGACAGAAG AAAGATCAGC CCTCTTTGGA
    AACATTCAGG ATATCTACCA TTTCAATAGT GAACTTCTAC AAGATTTGGA AAACTGTGAA
    AATGATCCTG TGGCCATTGC AGAATGTTTT GTATCCAAGA GTGAAGAGTT CCACATTTAC
    ACGCAGTACT GCACTAACTA TCCAAGATCA GTGGCTGTGC TGACAGAGTG CATGCGGAAC
    AAGATGCTGG CCAAATTCTT CAGGGAACGT CAGGAAACTT TGAAACACTC ACTGCCTTTG
    GGGTCCTATC TCTTGAAACC AGTTCAGCGG ATTCTCAAGT ATCATCTCCT TTTGCATGAA
    ATAGAAAATC ACCTTGACAA GGACACAGAA GGGTATGATG TGGTGCTTGA TGCAATAGAC
    ACGATGCAGC GAGTAGCCTG GCACATCAAC GACATGAAGA GGAAGCACGA ACACGCAGTC
    CGGTTACAGG AGATCCAGAG TCTGCTCACC AACTGGAAGG GGCCAGACCT GACCAGCTAT
    GGGGAACTGG TGCTCGAAGG AACCTTTCGC ATCCAGCGGG CCAAGAATGA GCGGACACTC
    TTCCTCTTTG ACAAGCTGCT GCTCATCACA AAGAAGAGAG ATGACACGTT TACATACAAA
    GCTCACATCC TTTGTGGCAA CCTCATGCTG GTGGAAGTGA TACCTAAGGA GCCGCTCAGC
    TTCAGCGTCT TCCACTATAA GAACCCAAAG CTGCAGCACA CAGTCCAGGC TAAATCCCAG
    CAGGACAAAC GCCTCTGGGT TCTGCACCTG AAGAGACTGA TCCTGGAGAA CCATGCGGCT
    AAGATTCCAG CTAAGGCCAA GCAAGCAATA CTTGAAATGG ATGCCATTCA TTATCCCGGT
    TTCTGTTACA GTCCTGAGGG AGAGACGAAA GCATTGTGTG GTTCTAAAGA TGGTTCGGCT
    CCGTATCGGC TGAGAAGAAA ATCTGAACCT TCCTCAAGAT CACATAAAGT TTTGAAGACC
    AGTGAAGCAG CACAAGACAT CCAAAAGGTT TCCAGAGAGG AAGGGTCTCC CCAGCGGACC
    TCAACCCGGC CATCTCCCGC CCAGAGGAAC AACCAGCCAA GCAGTTCTGC CATTATCAAC
    GTGCTTCGTG GGGGTGGAGC CATCAGAAAC ATCTGGACAG ACCACCAGAT CAGACAAGCC
    TTATTTCCCA GTCGTCGGTC CCCTCAGGAG AACGAGGATG ATGAAGATGA TTATCAGATG
    TTCGTGCCGT CCTTCTCCTC CTCAGATCTG AACTCTACCA GACTGTGTGA AGACAGCACT
    TCCAGTCGCC CTTGCAGTTG GCACATGGGA CAGATTGAGC CCACAGAGAC CACCAGCTCC
    AGCCACCGGG TTGTCAGGCG GGCCAGTAGC GCTGGTGAGA GCAATACATG CACTCCTGAA
    ATAAGAATTA GGGACAGTGG TGGCTCTCAG TACAGCCCCC GAAGGGAACT ACAGAGTGAC
    CCTAAAACAG AAGGGCAGGA TGGAATGACT CCCTTTGGGT CATCTATAGA GTTGACTATT
    GATGATATAG ACCATGTCTA TGATAACATA AGTTACGAGG ACTTAAAACT AATGGTTGCA
    AAACGGGAAG AAGCTGAATC CACACCCCTC AAAATAGCCA GGGACTCTGT TCGCCCCAAG
    AGCACCCCAG AGCTAGCCTT CTCAAAGAAG CAAGCTGGCC ACGAGAAGAG CTCTCTGCAC
    ACAAAAAGGG ATGGAGCTCT AACAGGTTGC CAGGCATCCA ACCAAAGCAC ACACGAACTC
    CAGATGGTTG AGGAAAACAT CTATGATACC ATAGGGCTCC CAGATGCACC CTCGCTGGAT
    TTTAACTGCA GCAGCCTGAA GCGTCCCAAG AGGAGCACCT TCTTAGGTCT GGAAGCCGAC
    TTTGCCTGCT GTGACAGTCT GAGGCCCTTC ATTTCCCAGG ATAGCCTCCA GTTCAGTGAG
    GATGAAGCTC CTTACCACCA GGGGCCTTCT GATAATGATT ATTTGAGTTT GCTCTATAAC
    TCTTTCAGCT GTAACCTATC TATAGGTGAT AAATCTATAT CTGATAAGTT GTCTGAAGAA
    GTAGATGAAA TCTGGAATGA CCTGGAAAAT TACATCAAGA AAAACGAAGT CAAAGCTAGA
    GACCGGCTCC TCGCAGCATT TCCTGTCAGC AAAGATGACG TGCCAGACAG GCTGCACTCC
    GAGAGCACAC CTGAGCTGGG CAGAGATGCG GCGCATTCTG TGTCTACCCT CTCGCTTCCT
    GAAGGTCACA CCTTCCTCAC GCCCCTGAAA GACAAGCCTG GCAGAGCTGG CCGGGCCAAC
    TGCCCTTTTG AAGAGGACCT GATTTCTAAA GAAGCCTCCT TTATGAGTCT TAACCGGCTG
    TCTTTGGCCA GTGAAACACC TCCCATGGAA AACCCCTATG ACTTGGCCAA CAGCAGCAGT
    CTGTCCCAAT CAGACTTGGA AATCCCTGAC GCCGGGATGG ATGTCAGTGA TAAGACAAGA
    AGCAGGGTTT TTATGATGGC CAGGCAGTAT AGTCAGAAGA TCAAGAAGGC AAATCAACTT
    TTAAAAGTGA GAAGCCCAGA GTTGGAACAG CCACTGGCCA GCCAGCAGCA TAAATCCACG
    CACAAAGACC TGGCAGCCAT CTTGGAAGAA AAGAAGCAAG GGGGTCCTGC CATCGGTGCC
    AGGATCGCTG AGTATTCCCA GCTGTATGAC CAGATTGTGT TCAGAGAGAC TCCTTGTAAG
    ACTCAGAAGG ATGGCTGGGC CAGCCCTCAA GAGCCCTCCA ACCTCAGATC TACATCATCT
    TCCCAGGCCC AACTTAGCAG TGAGGATTGG CTTTTGCATT CAACCTATAG TAATGGAGAG
    TTAGTGGATT TCTGTCCCCG GCCAGAGCAA GACTTGAAGT CAAAACATCC TACTTTAGAG
    ATCAGTACAA AAAGCATTCC AAGACAGCTG TCCACAGCTT GCTCTGTGCC TTCTCTTCAA
    ACCTCTGACC CTCTGCTGTG CTCCTTGCAG AGACACAGTG TGATAGTCAG CCAGCCCAAC
    AAAGATAACT CGTATCAGGG CCACCTCTAC AATTCGTTGG GTCGGAAAAG CATCAGTGCG
    AAATCTCAGC CTTATAGCAG GTCTCAGTCC TCTTCTTCAA TCTTGATAAA CAAGTCGGTG
    GACTCCATCA GCTACCCTGG TGAAATGGGA AAGAAACAGC CACTGTCTAT CCACAAAAGT
    TCGAGGTGTG AGAGTCACCA GGATTTGCTG CCAGCTATGG CTGACCCGTG TCAGCAGGGC
    CCTGAAAAAC ACTCAGACCT CACGCTCCGA GACTCACAGA AGGTTCTGGT AGTCAATAGA
    AATGTACCCT TAAATGCCCA AATAGCCACA CAGAACTATT TTTCCAATTT CAAAGAGACT
    GAAGGAGACG AAGACGACTA TGTGGAAATA AGATCTGAAG AAGACGAGTC AGAAGTAGAG
    CCATCCCATG GTCGGAGGAA GAAATCTGAC CCAAGGATTG CAGAAGCTGA CTTTTCGGAG
    GACCCCTGCA CCAACAGCAC CCCTTACTCT TTGAATAGTC CATGCACTCC AAAAAAGCCG
    ATAAGTGACA AGCTTGGGAT TTCACCCTAC CTGGCATCGT ACAGCGATTC GGACAAACTG
    AACGACTATC TCTGGAGGGG CCCGTCTCCC AACCAGCAAA ATATTGTCCA GTCGCTGAGG
    GAAAAATTTC AGTGTCTCAG TTCAAGCAGC TTTGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe00948
    Clone ID Related Accession (Same CDS sequence) XM_541152.6 , XM_005615510.3 , XM_005615508.3 , XM_541152.5 , XM_005615508.2 , XM_005615509.2 , XM_005615510.2
    Accession Version XM_005615510.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4179bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product pleckstrin homology domain-containing family G member 1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726046.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005615510.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAGCTCT CGGACAGCGA CCGCCCGGTC AGCTTCGGCT CCACGTCGTC CTCGGCTTCT 
    TCCCGGGACA GCCACGGTTC CTTCGGCAGC AGAATGACCT TAGTCTCAAA CAGCCACTTG
    GGCTTGTTTC ACCAAGATAA GGAAGCCGGG GCCATAAAAC TGGAGCTGAT TCCAGCCAGG
    CCGCTTTCCA GCAGCGAGCT GCAGCGGGCC GGCCCCGCTG GGCCACAGGA CACGGGCGAC
    GGCGGCGACA GGCAGCCCCG GGCCCAGCGG AGAATGGAAG CAAACGGGGC CACCAGAGCA
    GGTGCAGAGT CGGCCACCAG CCCCAAGCTC CTGTATGTGG ATAGGGTTGT TCAGGAGATT
    CTGGAGACTG AGAGGACTTA CGTGCAAGAC TTGAAAAGCA TCGTAGAGGA TTACCTTGAC
    TGCATCAGGG ACCAAACAAA ACTTCCTCTG GGGACAGAAG AAAGATCAGC CCTCTTTGGA
    AACATTCAGG ATATCTACCA TTTCAATAGT GAACTTCTAC AAGATTTGGA AAACTGTGAA
    AATGATCCTG TGGCCATTGC AGAATGTTTT GTATCCAAGA GTGAAGAGTT CCACATTTAC
    ACGCAGTACT GCACTAACTA TCCAAGATCA GTGGCTGTGC TGACAGAGTG CATGCGGAAC
    AAGATGCTGG CCAAATTCTT CAGGGAACGT CAGGAAACTT TGAAACACTC ACTGCCTTTG
    GGGTCCTATC TCTTGAAACC AGTTCAGCGG ATTCTCAAGT ATCATCTCCT TTTGCATGAA
    ATAGAAAATC ACCTTGACAA GGACACAGAA GGGTATGATG TGGTGCTTGA TGCAATAGAC
    ACGATGCAGC GAGTAGCCTG GCACATCAAC GACATGAAGA GGAAGCACGA ACACGCAGTC
    CGGTTACAGG AGATCCAGAG TCTGCTCACC AACTGGAAGG GGCCAGACCT GACCAGCTAT
    GGGGAACTGG TGCTCGAAGG AACCTTTCGC ATCCAGCGGG CCAAGAATGA GCGGACACTC
    TTCCTCTTTG ACAAGCTGCT GCTCATCACA AAGAAGAGAG ATGACACGTT TACATACAAA
    GCTCACATCC TTTGTGGCAA CCTCATGCTG GTGGAAGTGA TACCTAAGGA GCCGCTCAGC
    TTCAGCGTCT TCCACTATAA GAACCCAAAG CTGCAGCACA CAGTCCAGGC TAAATCCCAG
    CAGGACAAAC GCCTCTGGGT TCTGCACCTG AAGAGACTGA TCCTGGAGAA CCATGCGGCT
    AAGATTCCAG CTAAGGCCAA GCAAGCAATA CTTGAAATGG ATGCCATTCA TTATCCCGGT
    TTCTGTTACA GTCCTGAGGG AGAGACGAAA GCATTGTGTG GTTCTAAAGA TGGTTCGGCT
    CCGTATCGGC TGAGAAGAAA ATCTGAACCT TCCTCAAGAT CACATAAAGT TTTGAAGACC
    AGTGAAGCAG CACAAGACAT CCAAAAGGTT TCCAGAGAGG AAGGGTCTCC CCAGCGGACC
    TCAACCCGGC CATCTCCCGC CCAGAGGAAC AACCAGCCAA GCAGTTCTGC CATTATCAAC
    GTGCTTCGTG GGGGTGGAGC CATCAGAAAC ATCTGGACAG ACCACCAGAT CAGACAAGCC
    TTATTTCCCA GTCGTCGGTC CCCTCAGGAG AACGAGGATG ATGAAGATGA TTATCAGATG
    TTCGTGCCGT CCTTCTCCTC CTCAGATCTG AACTCTACCA GACTGTGTGA AGACAGCACT
    TCCAGTCGCC CTTGCAGTTG GCACATGGGA CAGATTGAGC CCACAGAGAC CACCAGCTCC
    AGCCACCGGG TTGTCAGGCG GGCCAGTAGC GCTGGTGAGA GCAATACATG CACTCCTGAA
    ATAAGAATTA GGGACAGTGG TGGCTCTCAG TACAGCCCCC GAAGGGAACT ACAGAGTGAC
    CCTAAAACAG AAGGGCAGGA TGGAATGACT CCCTTTGGGT CATCTATAGA GTTGACTATT
    GATGATATAG ACCATGTCTA TGATAACATA AGTTACGAGG ACTTAAAACT AATGGTTGCA
    AAACGGGAAG AAGCTGAATC CACACCCCTC AAAATAGCCA GGGACTCTGT TCGCCCCAAG
    AGCACCCCAG AGCTAGCCTT CTCAAAGAAG CAAGCTGGCC ACGAGAAGAG CTCTCTGCAC
    ACAAAAAGGG ATGGAGCTCT AACAGGTTGC CAGGCATCCA ACCAAAGCAC ACACGAACTC
    CAGATGGTTG AGGAAAACAT CTATGATACC ATAGGGCTCC CAGATGCACC CTCGCTGGAT
    TTTAACTGCA GCAGCCTGAA GCGTCCCAAG AGGAGCACCT TCTTAGGTCT GGAAGCCGAC
    TTTGCCTGCT GTGACAGTCT GAGGCCCTTC ATTTCCCAGG ATAGCCTCCA GTTCAGTGAG
    GATGAAGCTC CTTACCACCA GGGGCCTTCT GATAATGATT ATTTGAGTTT GCTCTATAAC
    TCTTTCAGCT GTAACCTATC TATAGGTGAT AAATCTATAT CTGATAAGTT GTCTGAAGAA
    GTAGATGAAA TCTGGAATGA CCTGGAAAAT TACATCAAGA AAAACGAAGT CAAAGCTAGA
    GACCGGCTCC TCGCAGCATT TCCTGTCAGC AAAGATGACG TGCCAGACAG GCTGCACTCC
    GAGAGCACAC CTGAGCTGGG CAGAGATGCG GCGCATTCTG TGTCTACCCT CTCGCTTCCT
    GAAGGTCACA CCTTCCTCAC GCCCCTGAAA GACAAGCCTG GCAGAGCTGG CCGGGCCAAC
    TGCCCTTTTG AAGAGGACCT GATTTCTAAA GAAGCCTCCT TTATGAGTCT TAACCGGCTG
    TCTTTGGCCA GTGAAACACC TCCCATGGAA AACCCCTATG ACTTGGCCAA CAGCAGCAGT
    CTGTCCCAAT CAGACTTGGA AATCCCTGAC GCCGGGATGG ATGTCAGTGA TAAGACAAGA
    AGCAGGGTTT TTATGATGGC CAGGCAGTAT AGTCAGAAGA TCAAGAAGGC AAATCAACTT
    TTAAAAGTGA GAAGCCCAGA GTTGGAACAG CCACTGGCCA GCCAGCAGCA TAAATCCACG
    CACAAAGACC TGGCAGCCAT CTTGGAAGAA AAGAAGCAAG GGGGTCCTGC CATCGGTGCC
    AGGATCGCTG AGTATTCCCA GCTGTATGAC CAGATTGTGT TCAGAGAGAC TCCTTGTAAG
    ACTCAGAAGG ATGGCTGGGC CAGCCCTCAA GAGCCCTCCA ACCTCAGATC TACATCATCT
    TCCCAGGCCC AACTTAGCAG TGAGGATTGG CTTTTGCATT CAACCTATAG TAATGGAGAG
    TTAGTGGATT TCTGTCCCCG GCCAGAGCAA GACTTGAAGT CAAAACATCC TACTTTAGAG
    ATCAGTACAA AAAGCATTCC AAGACAGCTG TCCACAGCTT GCTCTGTGCC TTCTCTTCAA
    ACCTCTGACC CTCTGCTGTG CTCCTTGCAG AGACACAGTG TGATAGTCAG CCAGCCCAAC
    AAAGATAACT CGTATCAGGG CCACCTCTAC AATTCGTTGG GTCGGAAAAG CATCAGTGCG
    AAATCTCAGC CTTATAGCAG GTCTCAGTCC TCTTCTTCAA TCTTGATAAA CAAGTCGGTG
    GACTCCATCA GCTACCCTGG TGAAATGGGA AAGAAACAGC CACTGTCTAT CCACAAAAGT
    TCGAGGTGTG AGAGTCACCA GGATTTGCTG CCAGCTATGG CTGACCCGTG TCAGCAGGGC
    CCTGAAAAAC ACTCAGACCT CACGCTCCGA GACTCACAGA AGGTTCTGGT AGTCAATAGA
    AATGTACCCT TAAATGCCCA AATAGCCACA CAGAACTATT TTTCCAATTT CAAAGAGACT
    GAAGGAGACG AAGACGACTA TGTGGAAATA AGATCTGAAG AAGACGAGTC AGAAGTAGAG
    CCATCCCATG GTCGGAGGAA GAAATCTGAC CCAAGGATTG CAGAAGCTGA CTTTTCGGAG
    GACCCCTGCA CCAACAGCAC CCCTTACTCT TTGAATAGTC CATGCACTCC AAAAAAGCCG
    ATAAGTGACA AGCTTGGGAT TTCACCCTAC CTGGCATCGT ACAGCGATTC GGACAAACTG
    AACGACTATC TCTGGAGGGG CCCGTCTCCC AACCAGCAAA ATATTGTCCA GTCGCTGAGG
    GAAAAATTTC AGTGTCTCAG TTCAAGCAGC TTTGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005615567.1
    CDS246..4424
    Translation

    Target ORF information:

    RefSeq Version XM_005615510.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris pleckstrin homology domain containing, family G (with RhoGef domain) member 1 (PLEKHG1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005615510.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAGCTCT CGGACAGCGA CCGCCCGGTC AGCTTCGGCT CCACGTCGTC CTCGGCTTCT 
    TCCCGGGACA GCCACGGTTC CTTCGGCAGC AGAATGACCT TAGTCTCAAA CAGCCACTTG
    GGCTTGTTTC ACCAAGATAA GGAAGCCGGG GCCATAAAAC TGGAGCTGAT TCCAGCCAGG
    CCGCTTTCCA GCAGCGAGCT GCAGCGGGCC GGCCCCGCTG GGCCACAGGA CACGGGCGAC
    GGCGGCGACA GGCAGCCCCG GGCCCAGCGG AGAATGGAAG CAAACGGGGC CACCAGAGCA
    GGTGCAGAGT CGGCCACCAG CCCCAAGCTC CTGTATGTGG ATAGGGTTGT TCAGGAGATT
    CTGGAGACTG AGAGGACTTA CGTGCAAGAC TTGAAAAGCA TCGTAGAGGA TTACCTTGAC
    TGCATCAGGG ACCAAACAAA ACTTCCTCTG GGGACAGAAG AAAGATCAGC CCTCTTTGGA
    AACATTCAGG ATATCTACCA TTTCAATAGT GAACTTCTAC AAGATTTGGA AAACTGTGAA
    AATGATCCTG TGGCCATTGC AGAATGTTTT GTATCCAAGA GTGAAGAGTT CCACATTTAC
    ACGCAGTACT GCACTAACTA TCCAAGATCA GTGGCTGTGC TGACAGAGTG CATGCGGAAC
    AAGATGCTGG CCAAATTCTT CAGGGAACGT CAGGAAACTT TGAAACACTC ACTGCCTTTG
    GGGTCCTATC TCTTGAAACC AGTTCAGCGG ATTCTCAAGT ATCATCTCCT TTTGCATGAA
    ATAGAAAATC ACCTTGACAA GGACACAGAA GGGTATGATG TGGTGCTTGA TGCAATAGAC
    ACGATGCAGC GAGTAGCCTG GCACATCAAC GACATGAAGA GGAAGCACGA ACACGCAGTC
    CGGTTACAGG AGATCCAGAG TCTGCTCACC AACTGGAAGG GGCCAGACCT GACCAGCTAT
    GGGGAACTGG TGCTCGAAGG AACCTTTCGC ATCCAGCGGG CCAAGAATGA GCGGACACTC
    TTCCTCTTTG ACAAGCTGCT GCTCATCACA AAGAAGAGAG ATGACACGTT TACATACAAA
    GCTCACATCC TTTGTGGCAA CCTCATGCTG GTGGAAGTGA TACCTAAGGA GCCGCTCAGC
    TTCAGCGTCT TCCACTATAA GAACCCAAAG CTGCAGCACA CAGTCCAGGC TAAATCCCAG
    CAGGACAAAC GCCTCTGGGT TCTGCACCTG AAGAGACTGA TCCTGGAGAA CCATGCGGCT
    AAGATTCCAG CTAAGGCCAA GCAAGCAATA CTTGAAATGG ATGCCATTCA TTATCCCGGT
    TTCTGTTACA GTCCTGAGGG AGAGACGAAA GCATTGTGTG GTTCTAAAGA TGGTTCGGCT
    CCGTATCGGC TGAGAAGAAA ATCTGAACCT TCCTCAAGAT CACATAAAGT TTTGAAGACC
    AGTGAAGCAG CACAAGACAT CCAAAAGGTT TCCAGAGAGG AAGGGTCTCC CCAGCGGACC
    TCAACCCGGC CATCTCCCGC CCAGAGGAAC AACCAGCCAA GCAGTTCTGC CATTATCAAC
    GTGCTTCGTG GGGGTGGAGC CATCAGAAAC ATCTGGACAG ACCACCAGAT CAGACAAGCC
    TTATTTCCCA GTCGTCGGTC CCCTCAGGAG AACGAGGATG ATGAAGATGA TTATCAGATG
    TTCGTGCCGT CCTTCTCCTC CTCAGATCTG AACTCTACCA GACTGTGTGA AGACAGCACT
    TCCAGTCGCC CTTGCAGTTG GCACATGGGA CAGATTGAGC CCACAGAGAC CACCAGCTCC
    AGCCACCGGG TTGTCAGGCG GGCCAGTAGC GCTGGTGAGA GCAATACATG CACTCCTGAA
    ATAAGAATTA GGGACAGTGG TGGCTCTCAG TACAGCCCCC GAAGGGAACT ACAGAGTGAC
    CCTAAAACAG AAGGGCAGGA TGGAATGACT CCCTTTGGGT CATCTATAGA GTTGACTATT
    GATGATATAG ACCATGTCTA TGATAACATA AGTTACGAGG ACTTAAAACT AATGGTTGCA
    AAACGGGAAG AAGCTGAATC CACACCCCTC AAAATAGCCA GGGACTCTGT TCGCCCCAAG
    AGCACCCCAG AGCTAGCCTT CTCAAAGAAG CAAGCTGGCC ACGAGAAGAG CTCTCTGCAC
    ACAAAAAGGG ATGGAGCTCT AACAGGTTGC CAGGCATCCA ACCAAAGCAC ACACGAACTC
    CAGATGGTTG AGGAAAACAT CTATGATACC ATAGGGCTCC CAGATGCACC CTCGCTGGAT
    TTTAACTGCA GCAGCCTGAA GCGTCCCAAG AGGAGCACCT TCTTAGGTCT GGAAGCCGAC
    TTTGCCTGCT GTGACAGTCT GAGGCCCTTC ATTTCCCAGG ATAGCCTCCA GTTCAGTGAG
    GATGAAGCTC CTTACCACCA GGGGCCTTCT GATAATGATT ATTTGAGTTT GCTCTATAAC
    TCTTTCAGCT GTAACCTATC TATAGGTGAT AAATCTATAT CTGATAAGTT GTCTGAAGAA
    GTAGATGAAA TCTGGAATGA CCTGGAAAAT TACATCAAGA AAAACGAAGT CAAAGCTAGA
    GACCGGCTCC TCGCAGCATT TCCTGTCAGC AAAGATGACG TGCCAGACAG GCTGCACTCC
    GAGAGCACAC CTGAGCTGGG CAGAGATGCG GCGCATTCTG TGTCTACCCT CTCGCTTCCT
    GAAGGTCACA CCTTCCTCAC GCCCCTGAAA GACAAGCCTG GCAGAGCTGG CCGGGCCAAC
    TGCCCTTTTG AAGAGGACCT GATTTCTAAA GAAGCCTCCT TTATGAGTCT TAACCGGCTG
    TCTTTGGCCA GTGAAACACC TCCCATGGAA AACCCCTATG ACTTGGCCAA CAGCAGCAGT
    CTGTCCCAAT CAGACTTGGA AATCCCTGAC GCCGGGATGG ATGTCAGTGA TAAGACAAGA
    AGCAGGGTTT TTATGATGGC CAGGCAGTAT AGTCAGAAGA TCAAGAAGGC AAATCAACTT
    TTAAAAGTGA GAAGCCCAGA GTTGGAACAG CCACTGGCCA GCCAGCAGCA TAAATCCACG
    CACAAAGACC TGGCAGCCAT CTTGGAAGAA AAGAAGCAAG GGGGTCCTGC CATCGGTGCC
    AGGATCGCTG AGTATTCCCA GCTGTATGAC CAGATTGTGT TCAGAGAGAC TCCTTGTAAG
    ACTCAGAAGG ATGGCTGGGC CAGCCCTCAA GAGCCCTCCA ACCTCAGATC TACATCATCT
    TCCCAGGCCC AACTTAGCAG TGAGGATTGG CTTTTGCATT CAACCTATAG TAATGGAGAG
    TTAGTGGATT TCTGTCCCCG GCCAGAGCAA GACTTGAAGT CAAAACATCC TACTTTAGAG
    ATCAGTACAA AAAGCATTCC AAGACAGCTG TCCACAGCTT GCTCTGTGCC TTCTCTTCAA
    ACCTCTGACC CTCTGCTGTG CTCCTTGCAG AGACACAGTG TGATAGTCAG CCAGCCCAAC
    AAAGATAACT CGTATCAGGG CCACCTCTAC AATTCGTTGG GTCGGAAAAG CATCAGTGCG
    AAATCTCAGC CTTATAGCAG GTCTCAGTCC TCTTCTTCAA TCTTGATAAA CAAGTCGGTG
    GACTCCATCA GCTACCCTGG TGAAATGGGA AAGAAACAGC CACTGTCTAT CCACAAAAGT
    TCGAGGTGTG AGAGTCACCA GGATTTGCTG CCAGCTATGG CTGACCCGTG TCAGCAGGGC
    CCTGAAAAAC ACTCAGACCT CACGCTCCGA GACTCACAGA AGGTTCTGGT AGTCAATAGA
    AATGTACCCT TAAATGCCCA AATAGCCACA CAGAACTATT TTTCCAATTT CAAAGAGACT
    GAAGGAGACG AAGACGACTA TGTGGAAATA AGATCTGAAG AAGACGAGTC AGAAGTAGAG
    CCATCCCATG GTCGGAGGAA GAAATCTGAC CCAAGGATTG CAGAAGCTGA CTTTTCGGAG
    GACCCCTGCA CCAACAGCAC CCCTTACTCT TTGAATAGTC CATGCACTCC AAAAAAGCCG
    ATAAGTGACA AGCTTGGGAT TTCACCCTAC CTGGCATCGT ACAGCGATTC GGACAAACTG
    AACGACTATC TCTGGAGGGG CCCGTCTCCC AACCAGCAAA ATATTGTCCA GTCGCTGAGG
    GAAAAATTTC AGTGTCTCAG TTCAAGCAGC TTTGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe34452
    Clone ID Related Accession (Same CDS sequence) XM_014112767.1
    Accession Version XM_014112767.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4371bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product pleckstrin homology domain-containing family G member 1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726046.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCAAGGA GCAAGAAGAT GAATAAATCA CTGTTCGGTG AAGACGGACA TTGGGAGTCT 
    GAGCACAGAC ACTGGAAAGA CTTCAGCTGT AAATATGTGC AGCAGTTGTG TCTTGAAGTG
    CCATCAGCTG TGACAGAAAT TCCAGATCCC AAGACCACAG CCCGGCACTC GGGCTCCCAG
    GACCTGAGGA CCATGGAGCT CTCGGACAGC GACCGCCCGG TCAGCTTCGG CTCCACGTCG
    TCCTCGGCTT CTTCCCGGGA CAGCCACGGT TCCTTCGGCA GCAGAATGAC CTTAGTCTCA
    AACAGCCACT TGGGCTTGTT TCACCAAGAT AAGGAAGCCG GGGCCATAAA ACTGGAGCTG
    ATTCCAGCCA GGCCGCTTTC CAGCAGCGAG CTGCAGCGGG CCGGCCCCGC TGGGCCACAG
    GACACGGGCG ACGGCGGCGA CAGGCAGCCC CGGGCCCAGC GGAGAATGGA AGCAAACGGG
    GCCACCAGAG CAGGTGCAGA GTCGGCCACC AGCCCCAAGC TCCTGTATGT GGATAGGGTT
    GTTCAGGAGA TTCTGGAGAC TGAGAGGACT TACGTGCAAG ACTTGAAAAG CATCGTAGAG
    GATTACCTTG ACTGCATCAG GGACCAAACA AAACTTCCTC TGGGGACAGA AGAAAGATCA
    GCCCTCTTTG GAAACATTCA GGATATCTAC CATTTCAATA GTGAACTTCT ACAAGATTTG
    GAAAACTGTG AAAATGATCC TGTGGCCATT GCAGAATGTT TTGTATCCAA GAGTGAAGAG
    TTCCACATTT ACACGCAGTA CTGCACTAAC TATCCAAGAT CAGTGGCTGT GCTGACAGAG
    TGCATGCGGA ACAAGATGCT GGCCAAATTC TTCAGGGAAC GTCAGGAAAC TTTGAAACAC
    TCACTGCCTT TGGGGTCCTA TCTCTTGAAA CCAGTTCAGC GGATTCTCAA GTATCATCTC
    CTTTTGCATG AAATAGAAAA TCACCTTGAC AAGGACACAG AAGGGTATGA TGTGGTGCTT
    GATGCAATAG ACACGATGCA GCGAGTAGCC TGGCACATCA ACGACATGAA GAGGAAGCAC
    GAACACGCAG TCCGGTTACA GGAGATCCAG AGTCTGCTCA CCAACTGGAA GGGGCCAGAC
    CTGACCAGCT ATGGGGAACT GGTGCTCGAA GGAACCTTTC GCATCCAGCG GGCCAAGAAT
    GAGCGGACAC TCTTCCTCTT TGACAAGCTG CTGCTCATCA CAAAGAAGAG AGATGACACG
    TTTACATACA AAGCTCACAT CCTTTGTGGC AACCTCATGC TGGTGGAAGT GATACCTAAG
    GAGCCGCTCA GCTTCAGCGT CTTCCACTAT AAGAACCCAA AGCTGCAGCA CACAGTCCAG
    GCTAAATCCC AGCAGGACAA ACGCCTCTGG GTTCTGCACC TGAAGAGACT GATCCTGGAG
    AACCATGCGG CTAAGATTCC AGCTAAGGCC AAGCAAGCAA TACTTGAAAT GGATGCCATT
    CATTATCCCG GTTTCTGTTA CAGTCCTGAG GGAGAGACGA AAGCATTGTG TGGTTCTAAA
    GATGGTTCGG CTCCGTATCG GCTGAGAAGA AAATCTGAAC CTTCCTCAAG ATCACATAAA
    GTTTTGAAGA CCAGTGAAGC AGCACAAGAC ATCCAAAAGG TTTCCAGAGA GGAAGGGTCT
    CCCCAGCGGA CCTCAACCCG GCCATCTCCC GCCCAGAGGA ACAACCAGCC AAGCAGTTCT
    GCCATTATCA ACGTGCTTCG TGGGGGTGGA GCCATCAGAA ACATCTGGAC AGACCACCAG
    ATCAGACAAG CCTTATTTCC CAGTCGTCGG TCCCCTCAGG AGAACGAGGA TGATGAAGAT
    GATTATCAGA TGTTCGTGCC GTCCTTCTCC TCCTCAGATC TGAACTCTAC CAGACTGTGT
    GAAGACAGCA CTTCCAGTCG CCCTTGCAGT TGGCACATGG GACAGATTGA GCCCACAGAG
    ACCACCAGCT CCAGCCACCG GGTTGTCAGG CGGGCCAGTA GCGCTGGTGA GAGCAATACA
    TGCACTCCTG AAATAAGAAT TAGGGACAGT GGTGGCTCTC AGTACAGCCC CCGAAGGGAA
    CTACAGAGTG ACCCTAAAAC AGAAGGGCAG GATGGAATGA CTCCCTTTGG GTCATCTATA
    GAGTTGACTA TTGATGATAT AGACCATGTC TATGATAACA TAAGTTACGA GGACTTAAAA
    CTAATGGTTG CAAAACGGGA AGAAGCTGAA TCCACACCCC TCAAAATAGC CAGGGACTCT
    GTTCGCCCCA AGAGCACCCC AGAGCTAGCC TTCTCAAAGA AGCAAGCTGG CCACGAGAAG
    AGCTCTCTGC ACACAAAAAG GGATGGAGCT CTAACAGGTT GCCAGGCATC CAACCAAAGC
    ACACACGAAC TCCAGATGGT TGAGGAAAAC ATCTATGATA CCATAGGGCT CCCAGATGCA
    CCCTCGCTGG ATTTTAACTG CAGCAGCCTG AAGCGTCCCA AGAGGAGCAC CTTCTTAGGT
    CTGGAAGCCG ACTTTGCCTG CTGTGACAGT CTGAGGCCCT TCATTTCCCA GGATAGCCTC
    CAGTTCAGTG AGGATGAAGC TCCTTACCAC CAGGGGCCTT CTGATAATGA TTATTTGAGT
    TTGCTCTATA ACTCTTTCAG CTGTAACCTA TCTATAGGTG ATAAATCTAT ATCTGATAAG
    TTGTCTGAAG AAGTAGATGA AATCTGGAAT GACCTGGAAA ATTACATCAA GAAAAACGAA
    GTCAAAGCTA GAGACCGGCT CCTCGCAGCA TTTCCTGTCA GCAAAGATGA CGTGCCAGAC
    AGGCTGCACT CCGAGAGCAC ACCTGAGCTG GGCAGAGATG CGGCGCATTC TGTGTCTACC
    CTCTCGCTTC CTGAAGGTCA CACCTTCCTC ACGCCCCTGA AAGACAAGCC TGGCAGAGCT
    GGCCGGGCCA ACTGCCCTTT TGAAGAGGAC CTGATTTCTA AAGAAGCCTC CTTTATGAGT
    CTTAACCGGC TGTCTTTGGC CAGTGAAACA CCTCCCATGG AAAACCCCTA TGACTTGGCC
    AACAGCAGCA GTCTGTCCCA ATCAGACTTG GAAATCCCTG ACGCCGGGAT GGATGTCAGT
    GATAAGACAA GAAGCAGGGT TTTTATGATG GCCAGGCAGT ATAGTCAGAA GATCAAGAAG
    GCAAATCAAC TTTTAAAAGT GAGAAGCCCA GAGTTGGAAC AGCCACTGGC CAGCCAGCAG
    CATAAATCCA CGCACAAAGA CCTGGCAGCC ATCTTGGAAG AAAAGAAGCA AGGGGGTCCT
    GCCATCGGTG CCAGGATCGC TGAGTATTCC CAGCTGTATG ACCAGATTGT GTTCAGAGAG
    ACTCCTTGTA AGACTCAGAA GGATGGCTGG GCCAGCCCTC AAGAGCCCTC CAACCTCAGA
    TCTACATCAT CTTCCCAGGC CCAACTTAGC AGTGAGGATT GGCTTTTGCA TTCAACCTAT
    AGTAATGGAG AGTTAGTGGA TTTCTGTCCC CGGCCAGAGC AAGACTTGAA GTCAAAACAT
    CCTACTTTAG AGATCAGTAC AAAAAGCATT CCAAGACAGC TGTCCACAGC TTGCTCTGTG
    CCTTCTCTTC AAACCTCTGA CCCTCTGCTG TGCTCCTTGC AGAGACACAG TGTGATAGTC
    AGCCAGCCCA ACAAAGATAA CTCGTATCAG GGCCACCTCT ACAATTCGTT GGGTCGGAAA
    AGCATCAGTG CGAAATCTCA GCCTTATAGC AGGTCTCAGT CCTCTTCTTC AATCTTGATA
    AACAAGTCGG TGGACTCCAT CAGCTACCCT GGTGAAATGG GAAAGAAACA GCCACTGTCT
    ATCCACAAAA GTTCGAGGTG TGAGAGTCAC CAGGATTTGC TGCCAGCTAT GGCTGACCCG
    TGTCAGCAGG GCCCTGAAAA ACACTCAGAC CTCACGCTCC GAGACTCACA GAAGGTTCTG
    GTAGTCAATA GAAATGTACC CTTAAATGCC CAAATAGCCA CACAGAACTA TTTTTCCAAT
    TTCAAAGAGA CTGAAGGAGA CGAAGACGAC TATGTGGAAA TAAGATCTGA AGAAGACGAG
    TCAGAAGTAG AGCCATCCCA TGGTCGGAGG AAGAAATCTG ACCCAAGGAT TGCAGAAGCT
    GACTTTTCGG AGGACCCCTG CACCAACAGC ACCCCTTACT CTTTGAATAG TCCATGCACT
    CCAAAAAAGC CGATAAGTGA CAAGCTTGGG ATTTCACCCT ACCTGGCATC GTACAGCGAT
    TCGGACAAAC TGAACGACTA TCTCTGGAGG GGCCCGTCTC CCAACCAGCA AAATATTGTC
    CAGTCGCTGA GGGAAAAATT TCAGTGTCTC AGTTCAAGCA GCTTTGCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013968242.1
    CDS16..4386
    Translation

    Target ORF information:

    RefSeq Version XM_014112767.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris pleckstrin homology domain containing, family G (with RhoGef domain) member 1 (PLEKHG1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014112767.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCAAGGA GCAAGAAGAT GAATAAATCA CTGTTCGGTG AAGACGGACA TTGGGAGTCT 
    GAGCACAGAC ACTGGAAAGA CTTCAGCTGT AAATATGTGC AGCAGTTGTG TCTTGAAGTG
    CCATCAGCTG TGACAGAAAT TCCAGATCCC AAGACCACAG CCCGGCACTC GGGCTCCCAG
    GACCTGAGGA CCATGGAGCT CTCGGACAGC GACCGCCCGG TCAGCTTCGG CTCCACGTCG
    TCCTCGGCTT CTTCCCGGGA CAGCCACGGT TCCTTCGGCA GCAGAATGAC CTTAGTCTCA
    AACAGCCACT TGGGCTTGTT TCACCAAGAT AAGGAAGCCG GGGCCATAAA ACTGGAGCTG
    ATTCCAGCCA GGCCGCTTTC CAGCAGCGAG CTGCAGCGGG CCGGCCCCGC TGGGCCACAG
    GACACGGGCG ACGGCGGCGA CAGGCAGCCC CGGGCCCAGC GGAGAATGGA AGCAAACGGG
    GCCACCAGAG CAGGTGCAGA GTCGGCCACC AGCCCCAAGC TCCTGTATGT GGATAGGGTT
    GTTCAGGAGA TTCTGGAGAC TGAGAGGACT TACGTGCAAG ACTTGAAAAG CATCGTAGAG
    GATTACCTTG ACTGCATCAG GGACCAAACA AAACTTCCTC TGGGGACAGA AGAAAGATCA
    GCCCTCTTTG GAAACATTCA GGATATCTAC CATTTCAATA GTGAACTTCT ACAAGATTTG
    GAAAACTGTG AAAATGATCC TGTGGCCATT GCAGAATGTT TTGTATCCAA GAGTGAAGAG
    TTCCACATTT ACACGCAGTA CTGCACTAAC TATCCAAGAT CAGTGGCTGT GCTGACAGAG
    TGCATGCGGA ACAAGATGCT GGCCAAATTC TTCAGGGAAC GTCAGGAAAC TTTGAAACAC
    TCACTGCCTT TGGGGTCCTA TCTCTTGAAA CCAGTTCAGC GGATTCTCAA GTATCATCTC
    CTTTTGCATG AAATAGAAAA TCACCTTGAC AAGGACACAG AAGGGTATGA TGTGGTGCTT
    GATGCAATAG ACACGATGCA GCGAGTAGCC TGGCACATCA ACGACATGAA GAGGAAGCAC
    GAACACGCAG TCCGGTTACA GGAGATCCAG AGTCTGCTCA CCAACTGGAA GGGGCCAGAC
    CTGACCAGCT ATGGGGAACT GGTGCTCGAA GGAACCTTTC GCATCCAGCG GGCCAAGAAT
    GAGCGGACAC TCTTCCTCTT TGACAAGCTG CTGCTCATCA CAAAGAAGAG AGATGACACG
    TTTACATACA AAGCTCACAT CCTTTGTGGC AACCTCATGC TGGTGGAAGT GATACCTAAG
    GAGCCGCTCA GCTTCAGCGT CTTCCACTAT AAGAACCCAA AGCTGCAGCA CACAGTCCAG
    GCTAAATCCC AGCAGGACAA ACGCCTCTGG GTTCTGCACC TGAAGAGACT GATCCTGGAG
    AACCATGCGG CTAAGATTCC AGCTAAGGCC AAGCAAGCAA TACTTGAAAT GGATGCCATT
    CATTATCCCG GTTTCTGTTA CAGTCCTGAG GGAGAGACGA AAGCATTGTG TGGTTCTAAA
    GATGGTTCGG CTCCGTATCG GCTGAGAAGA AAATCTGAAC CTTCCTCAAG ATCACATAAA
    GTTTTGAAGA CCAGTGAAGC AGCACAAGAC ATCCAAAAGG TTTCCAGAGA GGAAGGGTCT
    CCCCAGCGGA CCTCAACCCG GCCATCTCCC GCCCAGAGGA ACAACCAGCC AAGCAGTTCT
    GCCATTATCA ACGTGCTTCG TGGGGGTGGA GCCATCAGAA ACATCTGGAC AGACCACCAG
    ATCAGACAAG CCTTATTTCC CAGTCGTCGG TCCCCTCAGG AGAACGAGGA TGATGAAGAT
    GATTATCAGA TGTTCGTGCC GTCCTTCTCC TCCTCAGATC TGAACTCTAC CAGACTGTGT
    GAAGACAGCA CTTCCAGTCG CCCTTGCAGT TGGCACATGG GACAGATTGA GCCCACAGAG
    ACCACCAGCT CCAGCCACCG GGTTGTCAGG CGGGCCAGTA GCGCTGGTGA GAGCAATACA
    TGCACTCCTG AAATAAGAAT TAGGGACAGT GGTGGCTCTC AGTACAGCCC CCGAAGGGAA
    CTACAGAGTG ACCCTAAAAC AGAAGGGCAG GATGGAATGA CTCCCTTTGG GTCATCTATA
    GAGTTGACTA TTGATGATAT AGACCATGTC TATGATAACA TAAGTTACGA GGACTTAAAA
    CTAATGGTTG CAAAACGGGA AGAAGCTGAA TCCACACCCC TCAAAATAGC CAGGGACTCT
    GTTCGCCCCA AGAGCACCCC AGAGCTAGCC TTCTCAAAGA AGCAAGCTGG CCACGAGAAG
    AGCTCTCTGC ACACAAAAAG GGATGGAGCT CTAACAGGTT GCCAGGCATC CAACCAAAGC
    ACACACGAAC TCCAGATGGT TGAGGAAAAC ATCTATGATA CCATAGGGCT CCCAGATGCA
    CCCTCGCTGG ATTTTAACTG CAGCAGCCTG AAGCGTCCCA AGAGGAGCAC CTTCTTAGGT
    CTGGAAGCCG ACTTTGCCTG CTGTGACAGT CTGAGGCCCT TCATTTCCCA GGATAGCCTC
    CAGTTCAGTG AGGATGAAGC TCCTTACCAC CAGGGGCCTT CTGATAATGA TTATTTGAGT
    TTGCTCTATA ACTCTTTCAG CTGTAACCTA TCTATAGGTG ATAAATCTAT ATCTGATAAG
    TTGTCTGAAG AAGTAGATGA AATCTGGAAT GACCTGGAAA ATTACATCAA GAAAAACGAA
    GTCAAAGCTA GAGACCGGCT CCTCGCAGCA TTTCCTGTCA GCAAAGATGA CGTGCCAGAC
    AGGCTGCACT CCGAGAGCAC ACCTGAGCTG GGCAGAGATG CGGCGCATTC TGTGTCTACC
    CTCTCGCTTC CTGAAGGTCA CACCTTCCTC ACGCCCCTGA AAGACAAGCC TGGCAGAGCT
    GGCCGGGCCA ACTGCCCTTT TGAAGAGGAC CTGATTTCTA AAGAAGCCTC CTTTATGAGT
    CTTAACCGGC TGTCTTTGGC CAGTGAAACA CCTCCCATGG AAAACCCCTA TGACTTGGCC
    AACAGCAGCA GTCTGTCCCA ATCAGACTTG GAAATCCCTG ACGCCGGGAT GGATGTCAGT
    GATAAGACAA GAAGCAGGGT TTTTATGATG GCCAGGCAGT ATAGTCAGAA GATCAAGAAG
    GCAAATCAAC TTTTAAAAGT GAGAAGCCCA GAGTTGGAAC AGCCACTGGC CAGCCAGCAG
    CATAAATCCA CGCACAAAGA CCTGGCAGCC ATCTTGGAAG AAAAGAAGCA AGGGGGTCCT
    GCCATCGGTG CCAGGATCGC TGAGTATTCC CAGCTGTATG ACCAGATTGT GTTCAGAGAG
    ACTCCTTGTA AGACTCAGAA GGATGGCTGG GCCAGCCCTC AAGAGCCCTC CAACCTCAGA
    TCTACATCAT CTTCCCAGGC CCAACTTAGC AGTGAGGATT GGCTTTTGCA TTCAACCTAT
    AGTAATGGAG AGTTAGTGGA TTTCTGTCCC CGGCCAGAGC AAGACTTGAA GTCAAAACAT
    CCTACTTTAG AGATCAGTAC AAAAAGCATT CCAAGACAGC TGTCCACAGC TTGCTCTGTG
    CCTTCTCTTC AAACCTCTGA CCCTCTGCTG TGCTCCTTGC AGAGACACAG TGTGATAGTC
    AGCCAGCCCA ACAAAGATAA CTCGTATCAG GGCCACCTCT ACAATTCGTT GGGTCGGAAA
    AGCATCAGTG CGAAATCTCA GCCTTATAGC AGGTCTCAGT CCTCTTCTTC AATCTTGATA
    AACAAGTCGG TGGACTCCAT CAGCTACCCT GGTGAAATGG GAAAGAAACA GCCACTGTCT
    ATCCACAAAA GTTCGAGGTG TGAGAGTCAC CAGGATTTGC TGCCAGCTAT GGCTGACCCG
    TGTCAGCAGG GCCCTGAAAA ACACTCAGAC CTCACGCTCC GAGACTCACA GAAGGTTCTG
    GTAGTCAATA GAAATGTACC CTTAAATGCC CAAATAGCCA CACAGAACTA TTTTTCCAAT
    TTCAAAGAGA CTGAAGGAGA CGAAGACGAC TATGTGGAAA TAAGATCTGA AGAAGACGAG
    TCAGAAGTAG AGCCATCCCA TGGTCGGAGG AAGAAATCTG ACCCAAGGAT TGCAGAAGCT
    GACTTTTCGG AGGACCCCTG CACCAACAGC ACCCCTTACT CTTTGAATAG TCCATGCACT
    CCAAAAAAGC CGATAAGTGA CAAGCTTGGG ATTTCACCCT ACCTGGCATC GTACAGCGAT
    TCGGACAAAC TGAACGACTA TCTCTGGAGG GGCCCGTCTC CCAACCAGCA AAATATTGTC
    CAGTCGCTGA GGGAAAAATT TCAGTGTCTC AGTTCAAGCA GCTTTGCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.