plekhg1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	plekhg1
Entrez Gene ID	100488098
Full Name	pleckstrin homology and RhoGEF domain containing G1
General protein information	Preferred Names pleckstrin homology and RhoGEF domain containing G1 Names pleckstrin homology domain-containing family G member 1 pleckstrin homology domain containing, family G (with RhoGef domain) member 1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018094254.1	XP_017949743.1	pleckstrin homology domain-containing family G member 1 isoform X3
XM_031901989.1	XP_031757849.1	pleckstrin homology domain-containing family G member 1 isoform X4
XM_012963786.2	XP_012819240.1	pleckstrin homology domain-containing family G member 1 isoform X2
XM_018094254.2	XP_017949743.1	pleckstrin homology domain-containing family G member 1 isoform X3
XM_012963787.1	XP_012819241.1	pleckstrin homology domain-containing family G member 1 isoform X4
XM_012963786.3	XP_012819240.1	pleckstrin homology domain-containing family G member 1 isoform X2
XM_018094253.1	XP_017949742.1	pleckstrin homology domain-containing family G member 1 isoform X1
XM_018094253.2	XP_017949742.1	pleckstrin homology domain-containing family G member 1 isoform X1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	PLEKHG1	XP_419671.3
Xenopus tropicalis (tropical clawed frog)	plekhg1	XP_002934500.2
Pan troglodytes (chimpanzee)	PLEKHG1	XP_001135739.3
Bos taurus (cattle)	PLEKHG1	NP_001192850.1
Mus musculus (house mouse)	Plekhg1	NP_001153414.1
Rattus norvegicus (Norway rat)	Plekhg1	NP_001177928.1
	PLEKHG1	XP_001098617.2
Homo sapiens (human)	PLEKHG1	NP_001025055.1
Canis lupus familiaris (dog)	PLEKHG1	XP_005615564.1
Danio rerio (zebrafish)	LOC101886810	XP_005174146.1

Gene Ontology

The following plekhg1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the plekhg1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa34063	XM_012963787.1 Latest version!	Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G1 (plekhg1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa34062	XM_012963786.3 Latest version!	Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G1 (plekhg1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa44665	XM_018094253.2 Latest version!	Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G1 (plekhg1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa44666	XM_018094254.2 Latest version!	Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G1 (plekhg1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa58264	XM_031901989.1 Latest version!	Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G1 (plekhg1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
View Old Accession Versions >>
OXa44666	XM_018094254.1	Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G1 (plekhg1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OXa34062	XM_012963786.2	Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G1 (plekhg1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa44665	XM_018094253.1	Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G1 (plekhg1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

plekhg1 ( XM_012963787.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819241.1 Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G1 (plekhg1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa34063
Clone ID Related Accession (Same CDS sequence)	XM_012963787.1
Accession Version	XM_012963787.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3855bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology domain-containing family G member 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAACA AAATACTGGC CAAATTCTTT AGAGAACGAC AGGAAGTCCT ACAACATTCT 
CTTCCCTTAG GATCTTACCT CTTGAAGCCT GTCCAGAGAA TCCTTAAGTA CCACTTGCTG 
TTGCATGAAA TATCCAATCA TTTGGATAAA GACACTGAAG GCTATGACAT TGTGCTGGAT 
GCCATAGACA CAATGCAAAG GGTTGCATGG CATATAAATG ATATGAAGAG GAAACATGAA 
CATGACATTC GGCTCCAGGA AATCCAGAGT TTGCTCACTA ATTGGAAAGG ACCAGATCTG 
ACAAGTTACG GGGAGCTAGT TTTGGAAGGA ACATTCCGTT TTCAGAGGGC CAAGAATGAA 
CGCACTTTGT TCCTGTTCGA CCGTCTGCTT CTAATCACAA AGAAAAGGGA TGACACATAC 
ACATACAAGG CTCATATCCT GTGTTGCAAT CTCATGCTAG TTGAAGTAAT TCCAAAGGAG 
CCACTTAGCT TCAGTGTCTT CCATTATAAG AACCCAAAGC TACAGCACAC AGTACAGGCA 
AAGTCACAGC AAGACAAGAG GCTCTGGATT TTGCATTTGA AGAGACTTAT CCTTGAAAAC 
CACCCAGCGA AAATCCCAGC AAAGGCTAAA CAAGCAATTT TGGAAATGGA TGCTATACAT 
CATCCCGGGT TCTCCTATAC CCCAGAGGGA GAACGGAAAT CACCTTTAAA CTTGAAAGAA 
GATCTCGCAT CATGCAGAGT CAGAAGAAAG TCAGAACCTT CGTCTAATCT ACACAAAGTC 
ATGAAGCAAG ATGGTTTAAG CCCAGAGATG CAAAAGAGAA GTAGCATACA GGGCAATTTG 
CTATGCCAGG CTTCTAAACT CAGTGGAAGT GATCTGCTTC GTGGGTCTGG GATGAGAAAA 
GGTGGCTCAA TGAATCACAA CAGCAATATG ACCCGGTCTC AAGATTTCTA CAACAAAGAA 
GGTCTAAGTG TGCGCCTTGG GCCAGCGGAG CAGTCTGACG CAGATGATGA AGAGGAGTCT 
GAACAGCTGA GCTCTCAGCA TAAAATTAAA GGTGGCAGAA AAAGACTTAA CAGCCAAGCT 
TCAGAGAATG TTGAGAAGCG AAGAAGTATC AATCTAAGTG TATGTGAGAG CCAGATATCA 
AAAAGCCAGT CAGATGAAGA GTCAACAAAG CTGAATACAG GGCAGATCTT TCCAAGTTCC 
AGTGGTCATC TTCCAAGCAG ACAGAATGGT GCACAACAGA GCAATTCTTT GATTATGAAT 
ATCCTTGGAG GTACTGGTGC TCTGAGGAAC ATCTGGACTG ATCATCAAAT TAGACAAGCC 
CTCTTTCCTT CTAGACGCTC TACACAGGAA AATGAGGAGG ATGATGATGA CTACCAGATG 
TTTCTGTTGT CTGAATCTGC AACCAGGTTG GAATCGTCAT GGTCACAGGA AGAAAGAGTG 
GCATCAAGCC GGCCATGTAG CTGGCACCTT GGTCTTATGG AAGAAGACAC AATACCAAAC 
CCCTCCTGCA AAATTGTCAG GCGTGCAAGT AGTGTTGGAG AGAATAAATC TTGTTCTGCA 
AGACAACGTA GCCACTATCA TAAATCGGGG CCTCATGAAC ATTTCAATCG TTCACATGAG 
CGTCATCAAC ATTTTACTTG TCTTGGCTCT AATGAGGACT TAACCATTGA TGACATTGAA 
CATGTTTATG ACAATATTAG TTATGAAGAT CTGAAACAGA TGGGGTTCAC CAGGAGACAT 
GATTCTGATG CTCCTATAAA TAAGGAAGGA GGGGATTCTC AAAGCCAAGA AGACATTAGT 
ATTAGAAGAC TAAGAGAACA GTTTGATAGA ACCAGGAACT CCCTAATCCT AAATAAAGAG 
AAAAATCTAA AGAAAGGAAT GTCCAATTCA AATTCACAAG AACTCAGAAT AATTGAGGAA 
AACATTTATG ACTCTATTCA GCTACCAGAA CAATCTCTGG AGCATGTAAA GCATAAATTG 
CATTCTAAAC GGAGCAATTT TTTGGCTCTG GGCACTGATT TTCAGTCTTG TGATCAGCTT 
GATAGGTTTG TTTCCGAAGA CAGCCTACAG TTCAGCGAAG ATGAATCACT GGATCATCGT 
GTACCAGTGA AAGATGGAGA TTTCTGTTAT TCCTCTTCAA AGGCTGGTTC AGTATCACAT 
AAGTCTGCAG CAGATAAATT GTCAGAGGAA GTAGATGAAA TCTGGAATGA TTTGGAAAAC 
TACATTAAGA AGAATGAGGA GAAAAAAAGT GAACGGCTTT TAGCATCATT CCCTGTAAAC 
AAAGATGACG TTCTTGAAAG GCGCCATGTA ACGTACATGC CTTGTTTCAG CAAAGATATG 
GAGTATTCAT TGTCGTCATT GTGCTTGCCA GAAGATTTGC CTTCACAGAG GACAATACTG 
AATACTTCAC AAATAAGTAA TGATAATAGT AGCTGTGGAA GCTCAAATAG TGGCTCATTA 
CTAAGTCTTA AGAGGACTTC CATTGTAAGT GAAACAGGCT TTCTAGAATC ACCCCTTTTT 
GCTTCAAACA GCACTATTCC CTATTCCGAA GATACATGTT CTTCTCAAAC TTTAGAGAGC 
ACTGATAAAT CAAAAAGCAA GGTTTTTATG ATGGCTCGCC AATATAGTCA AAAAATAAAA 
AAAGCCAACC AGCTTTTAAA GATGAAAAGC CCAGAACAAG AACAGCCCTT TTACAAATCA 
CAGAAAATGA AACCGAAGGA TCTTGCAGCT ATTATGGAAG AGAAGAAACA AGGAGGCTGT 
GCTATTGGTG CAAGAATTGC CGAGTATTCC CAGCTTTATG ACCAGATTAT CTTTCGCGAC 
TCCCCCATAA GGATGCAAAA GGAAGCAAAG AAAGACTCCC ACAAAGTAGC ATCTCCAAGT 
TACTCTCCAT TAAGCCCAAC TTGCCAGCAA CCGTCCTCTC CATCTAAATG GCAACAGCGA 
GGTAAATGTC TCCAATCAAA TAGCACTGGT TTACAGTCTG TTAATGGAAG TGGGGCAGAA 
TTTTATACAT GGCCGGATGT AAAGGATTTT AAATCAAAAA CGGATTTATC AGAACCTGGC 
TCTGGGTGCC CTCACAGAAA CATTACAGCT GCTTGTTCAG TGCCATCTTT GTCAAAGTCA 
TTACAGGATG CATCGCATGA GTCAAGGTGG AGTGGCGTAA GCCAGCAGCA TAGTGAAAAT 
GGACAAATGG TTTGTGCTTC TAATTCACCA GAAAGGATAG TGAATAAAGA ACCTCAGCTT 
TATATTCGAT CACAGTCTTC ATCTAGTATG CTACATAACA GGACATCTGG CTCTCACAGA 
CTGGCAACAA ACTCACAGCG GTCAAAGCCC ATCTTAAAAC CTACAAGGCC TACAGATCTT 
GGTCATCTTG GATATGAAAG AAAATCCCAA ATTGAAGACG ACACCTCTGA TCTTACCTTG 
CAAGATTCCC AGAAGGTTTT GGTCATGAAC ACACTGTCCT CTCTTAATGC TCAAATAGCT 
ACGCTCAACT ATTATGCTAA CTTTAAGGAT ACTGATGGAG ATGACGATGA TGATGACTAT 
GTAGAGATCA AGTCAGAAGA TGAAGAGGAT GAGTTAGAGA CTGATTCTCA TCAGGCTCTT 
AAAATGAGTG TAAAAGGAAA GCCAAATGTT ACTCAGACTT ACTGTAGTAC AAATCCAACC 
TATTCCTTTA GCGTGCCCAG TACTCCAGTC AAGTCCTACA ACAGCAAGGA ACTCTCTGAT 
GCACATATAT TAGCCTATGA CGATGCAGAC AAACTAAATG ACTATCTCTG GAGACCAACG 
CCTTCTAATC AGCAAAATAT TGTTCAGTCA CTTAGAGAAA AATTTCAGTG TCTCAGCTCA 
AGTAGTTTTG CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819241.1
CDS	47..3901
Translation	MRNKILAKFFRERQEVLQHSLPLGSYLLKPVQRILKYHLLLHEISNHLDKDTEGYDIVLDAIDTMQRVAWHINDMKRKHEHDIRLQEIQSLLTNWKGPDLTSYGELVLEGTFRFQRAKNERTLFLFDRLLLITKKRDDTYTYKAHILCCNLMLVEVIPKEPLSFSVFHYKNPKLQHTVQAKSQQDKRLWILHLKRLILENHPAKIPAKAKQAILEMDAIHHPGFSYTPEGERKSPLNLKEDLASCRVRRKSEPSSNLHKVMKQDGLSPEMQKRSSIQGNLLCQASKLSGSDLLRGSGMRKGGSMNHNSNMTRSQDFYNKEGLSVRLGPAEQSDADDEEESEQLSSQHKIKGGRKRLNSQASENVEKRRSINLSVCESQISKSQSDEESTKLNTGQIFPSSSGHLPSRQNGAQQSNSLIMNILGGTGALRNIWTDHQIRQALFPSRRSTQENEEDDDDYQMFLLSESATRLESSWSQEERVASSRPCSWHLGLMEEDTIPNPSCKIVRRASSVGENKSCSARQRSHYHKSGPHEHFNRSHERHQHFTCLGSNEDLTIDDIEHVYDNISYEDLKQMGFTRRHDSDAPINKEGGDSQSQEDISIRRLREQFDRTRNSLILNKEKNLKKGMSNSNSQELRIIEENIYDSIQLPEQSLEHVKHKLHSKRSNFLALGTDFQSCDQLDRFVSEDSLQFSEDESLDHRVPVKDGDFCYSSSKAGSVSHKSAADKLSEEVDEIWNDLENYIKKNEEKKSERLLASFPVNKDDVLERRHVTYMPCFSKDMEYSLSSLCLPEDLPSQRTILNTSQISNDNSSCGSSNSGSLLSLKRTSIVSETGFLESPLFASNSTIPYSEDTCSSQTLESTDKSKSKVFMMARQYSQKIKKANQLLKMKSPEQEQPFYKSQKMKPKDLAAIMEEKKQGGCAIGARIAEYSQLYDQIIFRDSPIRMQKEAKKDSHKVASPSYSPLSPTCQQPSSPSKWQQRGKCLQSNSTGLQSVNGSGAEFYTWPDVKDFKSKTDLSEPGSGCPHRNITAACSVPSLSKSLQDASHESRWSGVSQQHSENGQMVCASNSPERIVNKEPQLYIRSQSSSSMLHNRTSGSHRLATNSQRSKPILKPTRPTDLGHLGYERKSQIEDDTSDLTLQDSQKVLVMNTLSSLNAQIATLNYYANFKDTDGDDDDDDYVEIKSEDEEDELETDSHQALKMSVKGKPNVTQTYCSTNPTYSFSVPSTPVKSYNSKELSDAHILAYDDADKLNDYLWRPTPSNQQNIVQSLREKFQCLSSSSFA

Target ORF information:

RefSeq Version	XM_012963787.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G1 (plekhg1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963787.1

ORF Insert Sequence:

ATGAGAAACA AAATACTGGC CAAATTCTTT AGAGAACGAC AGGAAGTCCT ACAACATTCT 
CTTCCCTTAG GATCTTACCT CTTGAAGCCT GTCCAGAGAA TCCTTAAGTA CCACTTGCTG 
TTGCATGAAA TATCCAATCA TTTGGATAAA GACACTGAAG GCTATGACAT TGTGCTGGAT 
GCCATAGACA CAATGCAAAG GGTTGCATGG CATATAAATG ATATGAAGAG GAAACATGAA 
CATGACATTC GGCTCCAGGA AATCCAGAGT TTGCTCACTA ATTGGAAAGG ACCAGATCTG 
ACAAGTTACG GGGAGCTAGT TTTGGAAGGA ACATTCCGTT TTCAGAGGGC CAAGAATGAA 
CGCACTTTGT TCCTGTTCGA CCGTCTGCTT CTAATCACAA AGAAAAGGGA TGACACATAC 
ACATACAAGG CTCATATCCT GTGTTGCAAT CTCATGCTAG TTGAAGTAAT TCCAAAGGAG 
CCACTTAGCT TCAGTGTCTT CCATTATAAG AACCCAAAGC TACAGCACAC AGTACAGGCA 
AAGTCACAGC AAGACAAGAG GCTCTGGATT TTGCATTTGA AGAGACTTAT CCTTGAAAAC 
CACCCAGCGA AAATCCCAGC AAAGGCTAAA CAAGCAATTT TGGAAATGGA TGCTATACAT 
CATCCCGGGT TCTCCTATAC CCCAGAGGGA GAACGGAAAT CACCTTTAAA CTTGAAAGAA 
GATCTCGCAT CATGCAGAGT CAGAAGAAAG TCAGAACCTT CGTCTAATCT ACACAAAGTC 
ATGAAGCAAG ATGGTTTAAG CCCAGAGATG CAAAAGAGAA GTAGCATACA GGGCAATTTG 
CTATGCCAGG CTTCTAAACT CAGTGGAAGT GATCTGCTTC GTGGGTCTGG GATGAGAAAA 
GGTGGCTCAA TGAATCACAA CAGCAATATG ACCCGGTCTC AAGATTTCTA CAACAAAGAA 
GGTCTAAGTG TGCGCCTTGG GCCAGCGGAG CAGTCTGACG CAGATGATGA AGAGGAGTCT 
GAACAGCTGA GCTCTCAGCA TAAAATTAAA GGTGGCAGAA AAAGACTTAA CAGCCAAGCT 
TCAGAGAATG TTGAGAAGCG AAGAAGTATC AATCTAAGTG TATGTGAGAG CCAGATATCA 
AAAAGCCAGT CAGATGAAGA GTCAACAAAG CTGAATACAG GGCAGATCTT TCCAAGTTCC 
AGTGGTCATC TTCCAAGCAG ACAGAATGGT GCACAACAGA GCAATTCTTT GATTATGAAT 
ATCCTTGGAG GTACTGGTGC TCTGAGGAAC ATCTGGACTG ATCATCAAAT TAGACAAGCC 
CTCTTTCCTT CTAGACGCTC TACACAGGAA AATGAGGAGG ATGATGATGA CTACCAGATG 
TTTCTGTTGT CTGAATCTGC AACCAGGTTG GAATCGTCAT GGTCACAGGA AGAAAGAGTG 
GCATCAAGCC GGCCATGTAG CTGGCACCTT GGTCTTATGG AAGAAGACAC AATACCAAAC 
CCCTCCTGCA AAATTGTCAG GCGTGCAAGT AGTGTTGGAG AGAATAAATC TTGTTCTGCA 
AGACAACGTA GCCACTATCA TAAATCGGGG CCTCATGAAC ATTTCAATCG TTCACATGAG 
CGTCATCAAC ATTTTACTTG TCTTGGCTCT AATGAGGACT TAACCATTGA TGACATTGAA 
CATGTTTATG ACAATATTAG TTATGAAGAT CTGAAACAGA TGGGGTTCAC CAGGAGACAT 
GATTCTGATG CTCCTATAAA TAAGGAAGGA GGGGATTCTC AAAGCCAAGA AGACATTAGT 
ATTAGAAGAC TAAGAGAACA GTTTGATAGA ACCAGGAACT CCCTAATCCT AAATAAAGAG 
AAAAATCTAA AGAAAGGAAT GTCCAATTCA AATTCACAAG AACTCAGAAT AATTGAGGAA 
AACATTTATG ACTCTATTCA GCTACCAGAA CAATCTCTGG AGCATGTAAA GCATAAATTG 
CATTCTAAAC GGAGCAATTT TTTGGCTCTG GGCACTGATT TTCAGTCTTG TGATCAGCTT 
GATAGGTTTG TTTCCGAAGA CAGCCTACAG TTCAGCGAAG ATGAATCACT GGATCATCGT 
GTACCAGTGA AAGATGGAGA TTTCTGTTAT TCCTCTTCAA AGGCTGGTTC AGTATCACAT 
AAGTCTGCAG CAGATAAATT GTCAGAGGAA GTAGATGAAA TCTGGAATGA TTTGGAAAAC 
TACATTAAGA AGAATGAGGA GAAAAAAAGT GAACGGCTTT TAGCATCATT CCCTGTAAAC 
AAAGATGACG TTCTTGAAAG GCGCCATGTA ACGTACATGC CTTGTTTCAG CAAAGATATG 
GAGTATTCAT TGTCGTCATT GTGCTTGCCA GAAGATTTGC CTTCACAGAG GACAATACTG 
AATACTTCAC AAATAAGTAA TGATAATAGT AGCTGTGGAA GCTCAAATAG TGGCTCATTA 
CTAAGTCTTA AGAGGACTTC CATTGTAAGT GAAACAGGCT TTCTAGAATC ACCCCTTTTT 
GCTTCAAACA GCACTATTCC CTATTCCGAA GATACATGTT CTTCTCAAAC TTTAGAGAGC 
ACTGATAAAT CAAAAAGCAA GGTTTTTATG ATGGCTCGCC AATATAGTCA AAAAATAAAA 
AAAGCCAACC AGCTTTTAAA GATGAAAAGC CCAGAACAAG AACAGCCCTT TTACAAATCA 
CAGAAAATGA AACCGAAGGA TCTTGCAGCT ATTATGGAAG AGAAGAAACA AGGAGGCTGT 
GCTATTGGTG CAAGAATTGC CGAGTATTCC CAGCTTTATG ACCAGATTAT CTTTCGCGAC 
TCCCCCATAA GGATGCAAAA GGAAGCAAAG AAAGACTCCC ACAAAGTAGC ATCTCCAAGT 
TACTCTCCAT TAAGCCCAAC TTGCCAGCAA CCGTCCTCTC CATCTAAATG GCAACAGCGA 
GGTAAATGTC TCCAATCAAA TAGCACTGGT TTACAGTCTG TTAATGGAAG TGGGGCAGAA 
TTTTATACAT GGCCGGATGT AAAGGATTTT AAATCAAAAA CGGATTTATC AGAACCTGGC 
TCTGGGTGCC CTCACAGAAA CATTACAGCT GCTTGTTCAG TGCCATCTTT GTCAAAGTCA 
TTACAGGATG CATCGCATGA GTCAAGGTGG AGTGGCGTAA GCCAGCAGCA TAGTGAAAAT 
GGACAAATGG TTTGTGCTTC TAATTCACCA GAAAGGATAG TGAATAAAGA ACCTCAGCTT 
TATATTCGAT CACAGTCTTC ATCTAGTATG CTACATAACA GGACATCTGG CTCTCACAGA 
CTGGCAACAA ACTCACAGCG GTCAAAGCCC ATCTTAAAAC CTACAAGGCC TACAGATCTT 
GGTCATCTTG GATATGAAAG AAAATCCCAA ATTGAAGACG ACACCTCTGA TCTTACCTTG 
CAAGATTCCC AGAAGGTTTT GGTCATGAAC ACACTGTCCT CTCTTAATGC TCAAATAGCT 
ACGCTCAACT ATTATGCTAA CTTTAAGGAT ACTGATGGAG ATGACGATGA TGATGACTAT 
GTAGAGATCA AGTCAGAAGA TGAAGAGGAT GAGTTAGAGA CTGATTCTCA TCAGGCTCTT 
AAAATGAGTG TAAAAGGAAA GCCAAATGTT ACTCAGACTT ACTGTAGTAC AAATCCAACC 
TATTCCTTTA GCGTGCCCAG TACTCCAGTC AAGTCCTACA ACAGCAAGGA ACTCTCTGAT 
GCACATATAT TAGCCTATGA CGATGCAGAC AAACTAAATG ACTATCTCTG GAGACCAACG 
CCTTCTAATC AGCAAAATAT TGTTCAGTCA CTTAGAGAAA AATTTCAGTG TCTCAGCTCA 
AGTAGTTTTG CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekhg1 ( XM_012963786.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819240.1 Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G1 (plekhg1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa34062
Clone ID Related Accession (Same CDS sequence)	XM_012963786.2 , XM_012963786.3
Accession Version	XM_012963786.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4653bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology domain-containing family G member 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012963786.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTACTG AGGACTACCA TTTGCCTCTT TATGGTCTTC CATCTGTAAG AGAGCCTCAG 
AGTCCTAGGA CAGCAGAGGA AAATCTGCAA AGGACCAGCA GACATTTCCG TTACTGTGGG 
ATCAAAAACA TGGAGATGTC AGACAGTGAC CGGCCAGTCA GCTTTGGTTC AACATCCTCA 
TCTGCCTCTT CCAGAGACAG TCACTGTTCA TTTGGCAGTC GGATGACCCT AGCATCAAAC 
AGTCACTTGG GTTTGTTTCA CCAAGATAAG GAAGCTGGAG CCATAAAACT AGAACTGGTT 
CCTGCCCGGC AGATATCAAA TGATGACCTT CATAGGATCT GCACCAAAGA ACAACCTCAT 
GGAAGTCCAA GGGAAAAACC ATCTGATGAG CATCAGAGCA CACAGTGGAA AGCTGAAGCT 
AAAAGATCAA TTAAATGTTC AGTGACTGAA GCGGTAGAGA CAACCAGTCC TAAACTTATG 
TATGTAGACA GAGTAGTTCA AGAAATTCTC CATACTGAAA GGGCCTATGT GCAGGATCTG 
AAAAGCATTG TTGAGGATTA TCTCAATTGC ATTTCTGACC AATCAAGACT TTCCCTAGGG 
ACAGAAGAAC GGTCAGCTCT CTTTGGCAAT ATATGCGATA TCTACCATTT CAATAGTGAA 
CTTCTTCAAG AACTGGAAAA TTGTGATAAT GATCCTGTTG CAATAGCCGA ATGCTTTGTG 
TCAAAGAGTG AGGAATTCCA TATATACACA CAGTATTGTA CTAATTATCC AAGATCTGTT 
GCTATATTGA CAGAGTGTAT GAGAAACAAA ATACTGGCCA AATTCTTTAG AGAACGACAG 
GAAGTCCTAC AACATTCTCT TCCCTTAGGA TCTTACCTCT TGAAGCCTGT CCAGAGAATC 
CTTAAGTACC ACTTGCTGTT GCATGAAATA TCCAATCATT TGGATAAAGA CACTGAAGGC 
TATGACATTG TGCTGGATGC CATAGACACA ATGCAAAGGG TTGCATGGCA TATAAATGAT 
ATGAAGAGGA AACATGAACA TGACATTCGG CTCCAGGAAA TCCAGAGTTT GCTCACTAAT 
TGGAAAGGAC CAGATCTGAC AAGTTACGGG GAGCTAGTTT TGGAAGGAAC ATTCCGTTTT 
CAGAGGGCCA AGAATGAACG CACTTTGTTC CTGTTCGACC GTCTGCTTCT AATCACAAAG 
AAAAGGGATG ACACATACAC ATACAAGGCT CATATCCTGT GTTGCAATCT CATGCTAGTT 
GAAGTAATTC CAAAGGAGCC ACTTAGCTTC AGTGTCTTCC ATTATAAGAA CCCAAAGCTA 
CAGCACACAG TACAGGCAAA GTCACAGCAA GACAAGAGGC TCTGGATTTT GCATTTGAAG 
AGACTTATCC TTGAAAACCA CCCAGCGAAA ATCCCAGCAA AGGCTAAACA AGCAATTTTG 
GAAATGGATG CTATACATCA TCCCGGGTTC TCCTATACCC CAGAGGGAGA ACGGAAATCA 
CCTTTAAACT TGAAAGAAGA TCTCGCATCA TGCAGAGTCA GAAGAAAGTC AGAACCTTCG 
TCTAATCTAC ACAAAGTCAT GAAGCAAGAT GGTTTAAGCC CAGAGATGCA AAAGAGAAGT 
AGCATACAGG GCAATTTGCT ATGCCAGGCT TCTAAACTCA GTGGAAGTGA TCTGCTTCGT 
GGGTCTGGGA TGAGAAAAGG TGGCTCAATG AATCACAACA GCAATATGAC CCGGTCTCAA 
GATTTCTACA ACAAAGAAGG TCTAAGTGTG CGCCTTGGGC CAGCGGAGCA GTCTGACGCA 
GATGATGAAG AGGAGTCTGA ACAGCTGAGC TCTCAGCATA AAATTAAAGG TGGCAGAAAA 
AGACTTAACA GCCAAGCTTC AGAGAATGTT GAGAAGCGAA GAAGTATCAA TCTAAGTGTA 
TGTGAGAGCC AGATATCAAA AAGCCAGTCA GATGAAGAGT CAACAAAGCT GAATACAGGG 
CAGATCTTTC CAAGTTCCAG TGGTCATCTT CCAAGCAGAC AGAATGGTGC ACAACAGAGC 
AATTCTTTGA TTATGAATAT CCTTGGAGGT ACTGGTGCTC TGAGGAACAT CTGGACTGAT 
CATCAAATTA GACAAGCCCT CTTTCCTTCT AGACGCTCTA CACAGGAAAA TGAGGAGGAT 
GATGATGACT ACCAGATGTT TCTGTTGTCT GAATCTGCAA CCAGGTTGGA ATCGTCATGG 
TCACAGGAAG AAAGAGTGGC ATCAAGCCGG CCATGTAGCT GGCACCTTGG TCTTATGGAA 
GAAGACACAA TACCAAACCC CTCCTGCAAA ATTGTCAGGC GTGCAAGTAG TGTTGGAGAG 
AATAAATCTT GTTCTGCAAG ACAACGTAGC CACTATCATA AATCGGGGCC TCATGAACAT 
TTCAATCGTT CACATGAGCG TCATCAACAT TTTACTTGTC TTGGCTCTAA TGAGGACTTA 
ACCATTGATG ACATTGAACA TGTTTATGAC AATATTAGTT ATGAAGATCT GAAACAGATG 
GGGTTCACCA GGAGACATGA TTCTGATGCT CCTATAAATA AGGAAGGAGG GGATTCTCAA 
AGCCAAGAAG ACATTAGTAT TAGAAGACTA AGAGAACAGT TTGATAGAAC CAGGAACTCC 
CTAATCCTAA ATAAAGAGAA AAATCTAAAG AAAGGAATGT CCAATTCAAA TTCACAAGAA 
CTCAGAATAA TTGAGGAAAA CATTTATGAC TCTATTCAGC TACCAGAACA ATCTCTGGAG 
CATGTAAAGC ATAAATTGCA TTCTAAACGG AGCAATTTTT TGGCTCTGGG CACTGATTTT 
CAGTCTTGTG ATCAGCTTGA TAGGTTTGTT TCCGAAGACA GCCTACAGTT CAGCGAAGAT 
GAATCACTGG ATCATCGTGT ACCAGTGAAA GATGGAGATT TCTGTTATTC CTCTTCAAAG 
GCTGGTTCAG TATCACATAA GTCTGCAGCA GATAAATTGT CAGAGGAAGT AGATGAAATC 
TGGAATGATT TGGAAAACTA CATTAAGAAG AATGAGGAGA AAAAAAGTGA ACGGCTTTTA 
GCATCATTCC CTGTAAACAA AGATGACGTT CTTGAAAGGC GCCATGTAAC GTACATGCCT 
TGTTTCAGCA AAGATATGGA GTATTCATTG TCGTCATTGT GCTTGCCAGA AGATTTGCCT 
TCACAGAGGA CAATACTGAA TACTTCACAA ATAAGTAATG ATAATAGTAG CTGTGGAAGC 
TCAAATAGTG GCTCATTACT AAGTCTTAAG AGGACTTCCA TTGTAAGTGA AACAGGCTTT 
CTAGAATCAC CCCTTTTTGC TTCAAACAGC ACTATTCCCT ATTCCGAAGA TACATGTTCT 
TCTCAAACTT TAGAGAGCAC TGATAAATCA AAAAGCAAGG TTTTTATGAT GGCTCGCCAA 
TATAGTCAAA AAATAAAAAA AGCCAACCAG CTTTTAAAGA TGAAAAGCCC AGAACAAGAA 
CAGCCCTTTT ACAAATCACA GAAAATGAAA CCGAAGGATC TTGCAGCTAT TATGGAAGAG 
AAGAAACAAG GAGGCTGTGC TATTGGTGCA AGAATTGCCG AGTATTCCCA GCTTTATGAC 
CAGATTATCT TTCGCGACTC CCCCATAAGG ATGCAAAAGG AAGCAAAGAA AGACTCCCAC 
AAAGTAGCAT CTCCAAGTTA CTCTCCATTA AGCCCAACTT GCCAGCAACC GTCCTCTCCA 
TCTAAATGGC AACAGCGAGG TAAATGTCTC CAATCAAATA GCACTGGTTT ACAGTCTGTT 
AATGGAAGTG GGGCAGAATT TTATACATGG CCGGATGTAA AGGATTTTAA ATCAAAAACG 
GATTTATCAG AACCTGGCTC TGGGTGCCCT CACAGAAACA TTACAGCTGC TTGTTCAGTG 
CCATCTTTGT CAAAGTCATT ACAGGATGCA TCGCATGAGT CAAGGTGGAG TGGCGTAAGC 
CAGCAGCATA GTGAAAATGG ACAAATGGTT TGTGCTTCTA ATTCACCAGA AAGGATAGTG 
AATAAAGAAC CTCAGCTTTA TATTCGATCA CAGTCTTCAT CTAGTATGCT ACATAACAGG 
ACATCTGGCT CTCACAGACT GGCAACAAAC TCACAGCGGT CAAAGCCCAT CTTAAAACCT 
ACAAGGCCTA CAGATCTTGG TCATCTTGGA TATGAAAGAA AATCCCAAAT TGAAGACGAC 
ACCTCTGATC TTACCTTGCA AGATTCCCAG AAGGTTTTGG TCATGAACAC ACTGTCCTCT 
CTTAATGCTC AAATAGCTAC GCTCAACTAT TATGCTAACT TTAAGGATAC TGATGGAGAT 
GACGATGATG ATGACTATGT AGAGATCAAG TCAGAAGATG AAGAGGATGA GTTAGAGACT 
GATTCTCATC AGGCTCTTAA AATGAGTGTA AAAGGAAAGC CAAATGTTAC TCAGACTTAC 
TGTAGTACAA ATCCAACCTA TTCCTTTAGC GTGCCCAGTA CTCCAGTCAA GTCCTACAAC 
AGCAAGGAAC TCTCTGATGC ACATATATTA GCCTATGACG ATGCAGACAA ACTAAATGAC 
TATCTCTGGA GACCAACGCC TTCTAATCAG CAAAATATTG TTCAGTCACT TAGAGAAAAA 
TTTCAGTGTC TCAGCTCAAG TAGTTTTGCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819240.1
CDS	651..5303
Translation	MPTEDYHLPLYGLPSVREPQSPRTAEENLQRTSRHFRYCGIKNMEMSDSDRPVSFGSTSSSASSRDSHCSFGSRMTLASNSHLGLFHQDKEAGAIKLELVPARQISNDDLHRICTKEQPHGSPREKPSDEHQSTQWKAEAKRSIKCSVTEAVETTSPKLMYVDRVVQEILHTERAYVQDLKSIVEDYLNCISDQSRLSLGTEERSALFGNICDIYHFNSELLQELENCDNDPVAIAECFVSKSEEFHIYTQYCTNYPRSVAILTECMRNKILAKFFRERQEVLQHSLPLGSYLLKPVQRILKYHLLLHEISNHLDKDTEGYDIVLDAIDTMQRVAWHINDMKRKHEHDIRLQEIQSLLTNWKGPDLTSYGELVLEGTFRFQRAKNERTLFLFDRLLLITKKRDDTYTYKAHILCCNLMLVEVIPKEPLSFSVFHYKNPKLQHTVQAKSQQDKRLWILHLKRLILENHPAKIPAKAKQAILEMDAIHHPGFSYTPEGERKSPLNLKEDLASCRVRRKSEPSSNLHKVMKQDGLSPEMQKRSSIQGNLLCQASKLSGSDLLRGSGMRKGGSMNHNSNMTRSQDFYNKEGLSVRLGPAEQSDADDEEESEQLSSQHKIKGGRKRLNSQASENVEKRRSINLSVCESQISKSQSDEESTKLNTGQIFPSSSGHLPSRQNGAQQSNSLIMNILGGTGALRNIWTDHQIRQALFPSRRSTQENEEDDDDYQMFLLSESATRLESSWSQEERVASSRPCSWHLGLMEEDTIPNPSCKIVRRASSVGENKSCSARQRSHYHKSGPHEHFNRSHERHQHFTCLGSNEDLTIDDIEHVYDNISYEDLKQMGFTRRHDSDAPINKEGGDSQSQEDISIRRLREQFDRTRNSLILNKEKNLKKGMSNSNSQELRIIEENIYDSIQLPEQSLEHVKHKLHSKRSNFLALGTDFQSCDQLDRFVSEDSLQFSEDESLDHRVPVKDGDFCYSSSKAGSVSHKSAADKLSEEVDEIWNDLENYIKKNEEKKSERLLASFPVNKDDVLERRHVTYMPCFSKDMEYSLSSLCLPEDLPSQRTILNTSQISNDNSSCGSSNSGSLLSLKRTSIVSETGFLESPLFASNSTIPYSEDTCSSQTLESTDKSKSKVFMMARQYSQKIKKANQLLKMKSPEQEQPFYKSQKMKPKDLAAIMEEKKQGGCAIGARIAEYSQLYDQIIFRDSPIRMQKEAKKDSHKVASPSYSPLSPTCQQPSSPSKWQQRGKCLQSNSTGLQSVNGSGAEFYTWPDVKDFKSKTDLSEPGSGCPHRNITAACSVPSLSKSLQDASHESRWSGVSQQHSENGQMVCASNSPERIVNKEPQLYIRSQSSSSMLHNRTSGSHRLATNSQRSKPILKPTRPTDLGHLGYERKSQIEDDTSDLTLQDSQKVLVMNTLSSLNAQIATLNYYANFKDTDGDDDDDDYVEIKSEDEEDELETDSHQALKMSVKGKPNVTQTYCSTNPTYSFSVPSTPVKSYNSKELSDAHILAYDDADKLNDYLWRPTPSNQQNIVQSLREKFQCLSSSSFA

Target ORF information:

RefSeq Version	XM_012963786.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G1 (plekhg1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963786.3

ORF Insert Sequence:

ATGCCTACTG AGGACTACCA TTTGCCTCTT TATGGTCTTC CATCTGTAAG AGAGCCTCAG 
AGTCCTAGGA CAGCAGAGGA AAATCTGCAA AGGACCAGCA GACATTTCCG TTACTGTGGG 
ATCAAAAACA TGGAGATGTC AGACAGTGAC CGGCCAGTCA GCTTTGGTTC AACATCCTCA 
TCTGCCTCTT CCAGAGACAG TCACTGTTCA TTTGGCAGTC GGATGACCCT AGCATCAAAC 
AGTCACTTGG GTTTGTTTCA CCAAGATAAG GAAGCTGGAG CCATAAAACT AGAACTGGTT 
CCTGCCCGGC AGATATCAAA TGATGACCTT CATAGGATCT GCACCAAAGA ACAACCTCAT 
GGAAGTCCAA GGGAAAAACC ATCTGATGAG CATCAGAGCA CACAGTGGAA AGCTGAAGCT 
AAAAGATCAA TTAAATGTTC AGTGACTGAA GCGGTAGAGA CAACCAGTCC TAAACTTATG 
TATGTAGACA GAGTAGTTCA AGAAATTCTC CATACTGAAA GGGCCTATGT GCAGGATCTG 
AAAAGCATTG TTGAGGATTA TCTCAATTGC ATTTCTGACC AATCAAGACT TTCCCTAGGG 
ACAGAAGAAC GGTCAGCTCT CTTTGGCAAT ATATGCGATA TCTACCATTT CAATAGTGAA 
CTTCTTCAAG AACTGGAAAA TTGTGATAAT GATCCTGTTG CAATAGCCGA ATGCTTTGTG 
TCAAAGAGTG AGGAATTCCA TATATACACA CAGTATTGTA CTAATTATCC AAGATCTGTT 
GCTATATTGA CAGAGTGTAT GAGAAACAAA ATACTGGCCA AATTCTTTAG AGAACGACAG 
GAAGTCCTAC AACATTCTCT TCCCTTAGGA TCTTACCTCT TGAAGCCTGT CCAGAGAATC 
CTTAAGTACC ACTTGCTGTT GCATGAAATA TCCAATCATT TGGATAAAGA CACTGAAGGC 
TATGACATTG TGCTGGATGC CATAGACACA ATGCAAAGGG TTGCATGGCA TATAAATGAT 
ATGAAGAGGA AACATGAACA TGACATTCGG CTCCAGGAAA TCCAGAGTTT GCTCACTAAT 
TGGAAAGGAC CAGATCTGAC AAGTTACGGG GAGCTAGTTT TGGAAGGAAC ATTCCGTTTT 
CAGAGGGCCA AGAATGAACG CACTTTGTTC CTGTTCGACC GTCTGCTTCT AATCACAAAG 
AAAAGGGATG ACACATACAC ATACAAGGCT CATATCCTGT GTTGCAATCT CATGCTAGTT 
GAAGTAATTC CAAAGGAGCC ACTTAGCTTC AGTGTCTTCC ATTATAAGAA CCCAAAGCTA 
CAGCACACAG TACAGGCAAA GTCACAGCAA GACAAGAGGC TCTGGATTTT GCATTTGAAG 
AGACTTATCC TTGAAAACCA CCCAGCGAAA ATCCCAGCAA AGGCTAAACA AGCAATTTTG 
GAAATGGATG CTATACATCA TCCCGGGTTC TCCTATACCC CAGAGGGAGA ACGGAAATCA 
CCTTTAAACT TGAAAGAAGA TCTCGCATCA TGCAGAGTCA GAAGAAAGTC AGAACCTTCG 
TCTAATCTAC ACAAAGTCAT GAAGCAAGAT GGTTTAAGCC CAGAGATGCA AAAGAGAAGT 
AGCATACAGG GCAATTTGCT ATGCCAGGCT TCTAAACTCA GTGGAAGTGA TCTGCTTCGT 
GGGTCTGGGA TGAGAAAAGG TGGCTCAATG AATCACAACA GCAATATGAC CCGGTCTCAA 
GATTTCTACA ACAAAGAAGG TCTAAGTGTG CGCCTTGGGC CAGCGGAGCA GTCTGACGCA 
GATGATGAAG AGGAGTCTGA ACAGCTGAGC TCTCAGCATA AAATTAAAGG TGGCAGAAAA 
AGACTTAACA GCCAAGCTTC AGAGAATGTT GAGAAGCGAA GAAGTATCAA TCTAAGTGTA 
TGTGAGAGCC AGATATCAAA AAGCCAGTCA GATGAAGAGT CAACAAAGCT GAATACAGGG 
CAGATCTTTC CAAGTTCCAG TGGTCATCTT CCAAGCAGAC AGAATGGTGC ACAACAGAGC 
AATTCTTTGA TTATGAATAT CCTTGGAGGT ACTGGTGCTC TGAGGAACAT CTGGACTGAT 
CATCAAATTA GACAAGCCCT CTTTCCTTCT AGACGCTCTA CACAGGAAAA TGAGGAGGAT 
GATGATGACT ACCAGATGTT TCTGTTGTCT GAATCTGCAA CCAGGTTGGA ATCGTCATGG 
TCACAGGAAG AAAGAGTGGC ATCAAGCCGG CCATGTAGCT GGCACCTTGG TCTTATGGAA 
GAAGACACAA TACCAAACCC CTCCTGCAAA ATTGTCAGGC GTGCAAGTAG TGTTGGAGAG 
AATAAATCTT GTTCTGCAAG ACAACGTAGC CACTATCATA AATCGGGGCC TCATGAACAT 
TTCAATCGTT CACATGAGCG TCATCAACAT TTTACTTGTC TTGGCTCTAA TGAGGACTTA 
ACCATTGATG ACATTGAACA TGTTTATGAC AATATTAGTT ATGAAGATCT GAAACAGATG 
GGGTTCACCA GGAGACATGA TTCTGATGCT CCTATAAATA AGGAAGGAGG GGATTCTCAA 
AGCCAAGAAG ACATTAGTAT TAGAAGACTA AGAGAACAGT TTGATAGAAC CAGGAACTCC 
CTAATCCTAA ATAAAGAGAA AAATCTAAAG AAAGGAATGT CCAATTCAAA TTCACAAGAA 
CTCAGAATAA TTGAGGAAAA CATTTATGAC TCTATTCAGC TACCAGAACA ATCTCTGGAG 
CATGTAAAGC ATAAATTGCA TTCTAAACGG AGCAATTTTT TGGCTCTGGG CACTGATTTT 
CAGTCTTGTG ATCAGCTTGA TAGGTTTGTT TCCGAAGACA GCCTACAGTT CAGCGAAGAT 
GAATCACTGG ATCATCGTGT ACCAGTGAAA GATGGAGATT TCTGTTATTC CTCTTCAAAG 
GCTGGTTCAG TATCACATAA GTCTGCAGCA GATAAATTGT CAGAGGAAGT AGATGAAATC 
TGGAATGATT TGGAAAACTA CATTAAGAAG AATGAGGAGA AAAAAAGTGA ACGGCTTTTA 
GCATCATTCC CTGTAAACAA AGATGACGTT CTTGAAAGGC GCCATGTAAC GTACATGCCT 
TGTTTCAGCA AAGATATGGA GTATTCATTG TCGTCATTGT GCTTGCCAGA AGATTTGCCT 
TCACAGAGGA CAATACTGAA TACTTCACAA ATAAGTAATG ATAATAGTAG CTGTGGAAGC 
TCAAATAGTG GCTCATTACT AAGTCTTAAG AGGACTTCCA TTGTAAGTGA AACAGGCTTT 
CTAGAATCAC CCCTTTTTGC TTCAAACAGC ACTATTCCCT ATTCCGAAGA TACATGTTCT 
TCTCAAACTT TAGAGAGCAC TGATAAATCA AAAAGCAAGG TTTTTATGAT GGCTCGCCAA 
TATAGTCAAA AAATAAAAAA AGCCAACCAG CTTTTAAAGA TGAAAAGCCC AGAACAAGAA 
CAGCCCTTTT ACAAATCACA GAAAATGAAA CCGAAGGATC TTGCAGCTAT TATGGAAGAG 
AAGAAACAAG GAGGCTGTGC TATTGGTGCA AGAATTGCCG AGTATTCCCA GCTTTATGAC 
CAGATTATCT TTCGCGACTC CCCCATAAGG ATGCAAAAGG AAGCAAAGAA AGACTCCCAC 
AAAGTAGCAT CTCCAAGTTA CTCTCCATTA AGCCCAACTT GCCAGCAACC GTCCTCTCCA 
TCTAAATGGC AACAGCGAGG TAAATGTCTC CAATCAAATA GCACTGGTTT ACAGTCTGTT 
AATGGAAGTG GGGCAGAATT TTATACATGG CCGGATGTAA AGGATTTTAA ATCAAAAACG 
GATTTATCAG AACCTGGCTC TGGGTGCCCT CACAGAAACA TTACAGCTGC TTGTTCAGTG 
CCATCTTTGT CAAAGTCATT ACAGGATGCA TCGCATGAGT CAAGGTGGAG TGGCGTAAGC 
CAGCAGCATA GTGAAAATGG ACAAATGGTT TGTGCTTCTA ATTCACCAGA AAGGATAGTG 
AATAAAGAAC CTCAGCTTTA TATTCGATCA CAGTCTTCAT CTAGTATGCT ACATAACAGG 
ACATCTGGCT CTCACAGACT GGCAACAAAC TCACAGCGGT CAAAGCCCAT CTTAAAACCT 
ACAAGGCCTA CAGATCTTGG TCATCTTGGA TATGAAAGAA AATCCCAAAT TGAAGACGAC 
ACCTCTGATC TTACCTTGCA AGATTCCCAG AAGGTTTTGG TCATGAACAC ACTGTCCTCT 
CTTAATGCTC AAATAGCTAC GCTCAACTAT TATGCTAACT TTAAGGATAC TGATGGAGAT 
GACGATGATG ATGACTATGT AGAGATCAAG TCAGAAGATG AAGAGGATGA GTTAGAGACT 
GATTCTCATC AGGCTCTTAA AATGAGTGTA AAAGGAAAGC CAAATGTTAC TCAGACTTAC 
TGTAGTACAA ATCCAACCTA TTCCTTTAGC GTGCCCAGTA CTCCAGTCAA GTCCTACAAC 
AGCAAGGAAC TCTCTGATGC ACATATATTA GCCTATGACG ATGCAGACAA ACTAAATGAC 
TATCTCTGGA GACCAACGCC TTCTAATCAG CAAAATATTG TTCAGTCACT TAGAGAAAAA 
TTTCAGTGTC TCAGCTCAAG TAGTTTTGCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekhg1 ( XM_018094253.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017949742.1 Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G1 (plekhg1), transcript variant X1, mRNA.

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CloneID	OXa44665
Clone ID Related Accession (Same CDS sequence)	XM_018094253.1 , XM_018094253.2
Accession Version	XM_018094253.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4692bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology domain-containing family G member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGATA AAGCACCAGA GGGTACAGAA ACAGCATCCC AGAATGAAGA GGACTACCAT 
TTGCCTCTTT ATGGTCTTCC ATCTGTAAGA GAGCCTCAGA GTCCTAGGAC AGCAGAGGAA 
AATCTGCAAA GGACCAGCAG ACATTTCCGT TACTGTGGGA TCAAAAACAT GGAGATGTCA 
GACAGTGACC GGCCAGTCAG CTTTGGTTCA ACATCCTCAT CTGCCTCTTC CAGAGACAGT 
CACTGTTCAT TTGGCAGTCG GATGACCCTA GCATCAAACA GTCACTTGGG TTTGTTTCAC 
CAAGATAAGG AAGCTGGAGC CATAAAACTA GAACTGGTTC CTGCCCGGCA GATATCAAAT 
GATGACCTTC ATAGGATCTG CACCAAAGAA CAACCTCATG GAAGTCCAAG GGAAAAACCA 
TCTGATGAGC ATCAGAGCAC ACAGTGGAAA GCTGAAGCTA AAAGATCAAT TAAATGTTCA 
GTGACTGAAG CGGTAGAGAC AACCAGTCCT AAACTTATGT ATGTAGACAG AGTAGTTCAA 
GAAATTCTCC ATACTGAAAG GGCCTATGTG CAGGATCTGA AAAGCATTGT TGAGGATTAT 
CTCAATTGCA TTTCTGACCA ATCAAGACTT TCCCTAGGGA CAGAAGAACG GTCAGCTCTC 
TTTGGCAATA TATGCGATAT CTACCATTTC AATAGTGAAC TTCTTCAAGA ACTGGAAAAT 
TGTGATAATG ATCCTGTTGC AATAGCCGAA TGCTTTGTGT CAAAGAGTGA GGAATTCCAT 
ATATACACAC AGTATTGTAC TAATTATCCA AGATCTGTTG CTATATTGAC AGAGTGTATG 
AGAAACAAAA TACTGGCCAA ATTCTTTAGA GAACGACAGG AAGTCCTACA ACATTCTCTT 
CCCTTAGGAT CTTACCTCTT GAAGCCTGTC CAGAGAATCC TTAAGTACCA CTTGCTGTTG 
CATGAAATAT CCAATCATTT GGATAAAGAC ACTGAAGGCT ATGACATTGT GCTGGATGCC 
ATAGACACAA TGCAAAGGGT TGCATGGCAT ATAAATGATA TGAAGAGGAA ACATGAACAT 
GACATTCGGC TCCAGGAAAT CCAGAGTTTG CTCACTAATT GGAAAGGACC AGATCTGACA 
AGTTACGGGG AGCTAGTTTT GGAAGGAACA TTCCGTTTTC AGAGGGCCAA GAATGAACGC 
ACTTTGTTCC TGTTCGACCG TCTGCTTCTA ATCACAAAGA AAAGGGATGA CACATACACA 
TACAAGGCTC ATATCCTGTG TTGCAATCTC ATGCTAGTTG AAGTAATTCC AAAGGAGCCA 
CTTAGCTTCA GTGTCTTCCA TTATAAGAAC CCAAAGCTAC AGCACACAGT ACAGGCAAAG 
TCACAGCAAG ACAAGAGGCT CTGGATTTTG CATTTGAAGA GACTTATCCT TGAAAACCAC 
CCAGCGAAAA TCCCAGCAAA GGCTAAACAA GCAATTTTGG AAATGGATGC TATACATCAT 
CCCGGGTTCT CCTATACCCC AGAGGGAGAA CGGAAATCAC CTTTAAACTT GAAAGAAGAT 
CTCGCATCAT GCAGAGTCAG AAGAAAGTCA GAACCTTCGT CTAATCTACA CAAAGTCATG 
AAGCAAGATG GTTTAAGCCC AGAGATGCAA AAGAGAAGTA GCATACAGGG CAATTTGCTA 
TGCCAGGCTT CTAAACTCAG TGGAAGTGAT CTGCTTCGTG GGTCTGGGAT GAGAAAAGGT 
GGCTCAATGA ATCACAACAG CAATATGACC CGGTCTCAAG ATTTCTACAA CAAAGAAGGT 
CTAAGTGTGC GCCTTGGGCC AGCGGAGCAG TCTGACGCAG ATGATGAAGA GGAGTCTGAA 
CAGCTGAGCT CTCAGCATAA AATTAAAGGT GGCAGAAAAA GACTTAACAG CCAAGCTTCA 
GAGAATGTTG AGAAGCGAAG AAGTATCAAT CTAAGTGTAT GTGAGAGCCA GATATCAAAA 
AGCCAGTCAG ATGAAGAGTC AACAAAGCTG AATACAGGGC AGATCTTTCC AAGTTCCAGT 
GGTCATCTTC CAAGCAGACA GAATGGTGCA CAACAGAGCA ATTCTTTGAT TATGAATATC 
CTTGGAGGTA CTGGTGCTCT GAGGAACATC TGGACTGATC ATCAAATTAG ACAAGCCCTC 
TTTCCTTCTA GACGCTCTAC ACAGGAAAAT GAGGAGGATG ATGATGACTA CCAGATGTTT 
CTGTTGTCTG AATCTGCAAC CAGGTTGGAA TCGTCATGGT CACAGGAAGA AAGAGTGGCA 
TCAAGCCGGC CATGTAGCTG GCACCTTGGT CTTATGGAAG AAGACACAAT ACCAAACCCC 
TCCTGCAAAA TTGTCAGGCG TGCAAGTAGT GTTGGAGAGA ATAAATCTTG TTCTGCAAGA 
CAACGTAGCC ACTATCATAA ATCGGGGCCT CATGAACATT TCAATCGTTC ACATGAGCGT 
CATCAACATT TTACTTGTCT TGGCTCTAAT GAGGACTTAA CCATTGATGA CATTGAACAT 
GTTTATGACA ATATTAGTTA TGAAGATCTG AAACAGATGG GGTTCACCAG GAGACATGAT 
TCTGATGCTC CTATAAATAA GGAAGGAGGG GATTCTCAAA GCCAAGAAGA CATTAGTATT 
AGAAGACTAA GAGAACAGTT TGATAGAACC AGGAACTCCC TAATCCTAAA TAAAGAGAAA 
AATCTAAAGA AAGGAATGTC CAATTCAAAT TCACAAGAAC TCAGAATAAT TGAGGAAAAC 
ATTTATGACT CTATTCAGCT ACCAGAACAA TCTCTGGAGC ATGTAAAGCA TAAATTGCAT 
TCTAAACGGA GCAATTTTTT GGCTCTGGGC ACTGATTTTC AGTCTTGTGA TCAGCTTGAT 
AGGTTTGTTT CCGAAGACAG CCTACAGTTC AGCGAAGATG AATCACTGGA TCATCGTGTA 
CCAGTGAAAG ATGGAGATTT CTGTTATTCC TCTTCAAAGG CTGGTTCAGT ATCACATAAG 
TCTGCAGCAG ATAAATTGTC AGAGGAAGTA GATGAAATCT GGAATGATTT GGAAAACTAC 
ATTAAGAAGA ATGAGGAGAA AAAAAGTGAA CGGCTTTTAG CATCATTCCC TGTAAACAAA 
GATGACGTTC TTGAAAGGCG CCATGTAACG TACATGCCTT GTTTCAGCAA AGATATGGAG 
TATTCATTGT CGTCATTGTG CTTGCCAGAA GATTTGCCTT CACAGAGGAC AATACTGAAT 
ACTTCACAAA TAAGTAATGA TAATAGTAGC TGTGGAAGCT CAAATAGTGG CTCATTACTA 
AGTCTTAAGA GGACTTCCAT TGTAAGTGAA ACAGGCTTTC TAGAATCACC CCTTTTTGCT 
TCAAACAGCA CTATTCCCTA TTCCGAAGAT ACATGTTCTT CTCAAACTTT AGAGAGCACT 
GATAAATCAA AAAGCAAGGT TTTTATGATG GCTCGCCAAT ATAGTCAAAA AATAAAAAAA 
GCCAACCAGC TTTTAAAGAT GAAAAGCCCA GAACAAGAAC AGCCCTTTTA CAAATCACAG 
AAAATGAAAC CGAAGGATCT TGCAGCTATT ATGGAAGAGA AGAAACAAGG AGGCTGTGCT 
ATTGGTGCAA GAATTGCCGA GTATTCCCAG CTTTATGACC AGATTATCTT TCGCGACTCC 
CCCATAAGGA TGCAAAAGGA AGCAAAGAAA GACTCCCACA AAGTAGCATC TCCAAGTTAC 
TCTCCATTAA GCCCAACTTG CCAGCAACCG TCCTCTCCAT CTAAATGGCA ACAGCGAGGT 
AAATGTCTCC AATCAAATAG CACTGGTTTA CAGTCTGTTA ATGGAAGTGG GGCAGAATTT 
TATACATGGC CGGATGTAAA GGATTTTAAA TCAAAAACGG ATTTATCAGA ACCTGGCTCT 
GGGTGCCCTC ACAGAAACAT TACAGCTGCT TGTTCAGTGC CATCTTTGTC AAAGTCATTA 
CAGGATGCAT CGCATGAGTC AAGGTGGAGT GGCGTAAGCC AGCAGCATAG TGAAAATGGA 
CAAATGGTTT GTGCTTCTAA TTCACCAGAA AGGATAGTGA ATAAAGAACC TCAGCTTTAT 
ATTCGATCAC AGTCTTCATC TAGTATGCTA CATAACAGGA CATCTGGCTC TCACAGACTG 
GCAACAAACT CACAGCGGTC AAAGCCCATC TTAAAACCTA CAAGGCCTAC AGATCTTGGT 
CATCTTGGAT ATGAAAGAAA ATCCCAAATT GAAGACGACA CCTCTGATCT TACCTTGCAA 
GATTCCCAGA AGGTTTTGGT CATGAACACA CTGTCCTCTC TTAATGCTCA AATAGCTACG 
CTCAACTATT ATGCTAACTT TAAGGATACT GATGGAGATG ACGATGATGA TGACTATGTA 
GAGATCAAGT CAGAAGATGA AGAGGATGAG TTAGAGACTG ATTCTCATCA GGCTCTTAAA 
ATGAGTGTAA AAGGAAAGCC AAATGTTACT CAGACTTACT GTAGTACAAA TCCAACCTAT 
TCCTTTAGCG TGCCCAGTAC TCCAGTCAAG TCCTACAACA GCAAGGAACT CTCTGATGCA 
CATATATTAG CCTATGACGA TGCAGACAAA CTAAATGACT ATCTCTGGAG ACCAACGCCT 
TCTAATCAGC AAAATATTGT TCAGTCACTT AGAGAAAAAT TTCAGTGTCT CAGCTCAAGT 
AGTTTTGCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017949742.1
CDS	235..4926
Translation	MPDKAPEGTETASQNEEDYHLPLYGLPSVREPQSPRTAEENLQRTSRHFRYCGIKNMEMSDSDRPVSFGSTSSSASSRDSHCSFGSRMTLASNSHLGLFHQDKEAGAIKLELVPARQISNDDLHRICTKEQPHGSPREKPSDEHQSTQWKAEAKRSIKCSVTEAVETTSPKLMYVDRVVQEILHTERAYVQDLKSIVEDYLNCISDQSRLSLGTEERSALFGNICDIYHFNSELLQELENCDNDPVAIAECFVSKSEEFHIYTQYCTNYPRSVAILTECMRNKILAKFFRERQEVLQHSLPLGSYLLKPVQRILKYHLLLHEISNHLDKDTEGYDIVLDAIDTMQRVAWHINDMKRKHEHDIRLQEIQSLLTNWKGPDLTSYGELVLEGTFRFQRAKNERTLFLFDRLLLITKKRDDTYTYKAHILCCNLMLVEVIPKEPLSFSVFHYKNPKLQHTVQAKSQQDKRLWILHLKRLILENHPAKIPAKAKQAILEMDAIHHPGFSYTPEGERKSPLNLKEDLASCRVRRKSEPSSNLHKVMKQDGLSPEMQKRSSIQGNLLCQASKLSGSDLLRGSGMRKGGSMNHNSNMTRSQDFYNKEGLSVRLGPAEQSDADDEEESEQLSSQHKIKGGRKRLNSQASENVEKRRSINLSVCESQISKSQSDEESTKLNTGQIFPSSSGHLPSRQNGAQQSNSLIMNILGGTGALRNIWTDHQIRQALFPSRRSTQENEEDDDDYQMFLLSESATRLESSWSQEERVASSRPCSWHLGLMEEDTIPNPSCKIVRRASSVGENKSCSARQRSHYHKSGPHEHFNRSHERHQHFTCLGSNEDLTIDDIEHVYDNISYEDLKQMGFTRRHDSDAPINKEGGDSQSQEDISIRRLREQFDRTRNSLILNKEKNLKKGMSNSNSQELRIIEENIYDSIQLPEQSLEHVKHKLHSKRSNFLALGTDFQSCDQLDRFVSEDSLQFSEDESLDHRVPVKDGDFCYSSSKAGSVSHKSAADKLSEEVDEIWNDLENYIKKNEEKKSERLLASFPVNKDDVLERRHVTYMPCFSKDMEYSLSSLCLPEDLPSQRTILNTSQISNDNSSCGSSNSGSLLSLKRTSIVSETGFLESPLFASNSTIPYSEDTCSSQTLESTDKSKSKVFMMARQYSQKIKKANQLLKMKSPEQEQPFYKSQKMKPKDLAAIMEEKKQGGCAIGARIAEYSQLYDQIIFRDSPIRMQKEAKKDSHKVASPSYSPLSPTCQQPSSPSKWQQRGKCLQSNSTGLQSVNGSGAEFYTWPDVKDFKSKTDLSEPGSGCPHRNITAACSVPSLSKSLQDASHESRWSGVSQQHSENGQMVCASNSPERIVNKEPQLYIRSQSSSSMLHNRTSGSHRLATNSQRSKPILKPTRPTDLGHLGYERKSQIEDDTSDLTLQDSQKVLVMNTLSSLNAQIATLNYYANFKDTDGDDDDDDYVEIKSEDEEDELETDSHQALKMSVKGKPNVTQTYCSTNPTYSFSVPSTPVKSYNSKELSDAHILAYDDADKLNDYLWRPTPSNQQNIVQSLREKFQCLSSSSFA

Target ORF information:

RefSeq Version	XM_018094253.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G1 (plekhg1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018094253.1

ORF Insert Sequence:

ATGCCAGATA AAGCACCAGA GGGTACAGAA ACAGCATCCC AGAATGAAGA GGACTACCAT 
TTGCCTCTTT ATGGTCTTCC ATCTGTAAGA GAGCCTCAGA GTCCTAGGAC AGCAGAGGAA 
AATCTGCAAA GGACCAGCAG ACATTTCCGT TACTGTGGGA TCAAAAACAT GGAGATGTCA 
GACAGTGACC GGCCAGTCAG CTTTGGTTCA ACATCCTCAT CTGCCTCTTC CAGAGACAGT 
CACTGTTCAT TTGGCAGTCG GATGACCCTA GCATCAAACA GTCACTTGGG TTTGTTTCAC 
CAAGATAAGG AAGCTGGAGC CATAAAACTA GAACTGGTTC CTGCCCGGCA GATATCAAAT 
GATGACCTTC ATAGGATCTG CACCAAAGAA CAACCTCATG GAAGTCCAAG GGAAAAACCA 
TCTGATGAGC ATCAGAGCAC ACAGTGGAAA GCTGAAGCTA AAAGATCAAT TAAATGTTCA 
GTGACTGAAG CGGTAGAGAC AACCAGTCCT AAACTTATGT ATGTAGACAG AGTAGTTCAA 
GAAATTCTCC ATACTGAAAG GGCCTATGTG CAGGATCTGA AAAGCATTGT TGAGGATTAT 
CTCAATTGCA TTTCTGACCA ATCAAGACTT TCCCTAGGGA CAGAAGAACG GTCAGCTCTC 
TTTGGCAATA TATGCGATAT CTACCATTTC AATAGTGAAC TTCTTCAAGA ACTGGAAAAT 
TGTGATAATG ATCCTGTTGC AATAGCCGAA TGCTTTGTGT CAAAGAGTGA GGAATTCCAT 
ATATACACAC AGTATTGTAC TAATTATCCA AGATCTGTTG CTATATTGAC AGAGTGTATG 
AGAAACAAAA TACTGGCCAA ATTCTTTAGA GAACGACAGG AAGTCCTACA ACATTCTCTT 
CCCTTAGGAT CTTACCTCTT GAAGCCTGTC CAGAGAATCC TTAAGTACCA CTTGCTGTTG 
CATGAAATAT CCAATCATTT GGATAAAGAC ACTGAAGGCT ATGACATTGT GCTGGATGCC 
ATAGACACAA TGCAAAGGGT TGCATGGCAT ATAAATGATA TGAAGAGGAA ACATGAACAT 
GACATTCGGC TCCAGGAAAT CCAGAGTTTG CTCACTAATT GGAAAGGACC AGATCTGACA 
AGTTACGGGG AGCTAGTTTT GGAAGGAACA TTCCGTTTTC AGAGGGCCAA GAATGAACGC 
ACTTTGTTCC TGTTCGACCG TCTGCTTCTA ATCACAAAGA AAAGGGATGA CACATACACA 
TACAAGGCTC ATATCCTGTG TTGCAATCTC ATGCTAGTTG AAGTAATTCC AAAGGAGCCA 
CTTAGCTTCA GTGTCTTCCA TTATAAGAAC CCAAAGCTAC AGCACACAGT ACAGGCAAAG 
TCACAGCAAG ACAAGAGGCT CTGGATTTTG CATTTGAAGA GACTTATCCT TGAAAACCAC 
CCAGCGAAAA TCCCAGCAAA GGCTAAACAA GCAATTTTGG AAATGGATGC TATACATCAT 
CCCGGGTTCT CCTATACCCC AGAGGGAGAA CGGAAATCAC CTTTAAACTT GAAAGAAGAT 
CTCGCATCAT GCAGAGTCAG AAGAAAGTCA GAACCTTCGT CTAATCTACA CAAAGTCATG 
AAGCAAGATG GTTTAAGCCC AGAGATGCAA AAGAGAAGTA GCATACAGGG CAATTTGCTA 
TGCCAGGCTT CTAAACTCAG TGGAAGTGAT CTGCTTCGTG GGTCTGGGAT GAGAAAAGGT 
GGCTCAATGA ATCACAACAG CAATATGACC CGGTCTCAAG ATTTCTACAA CAAAGAAGGT 
CTAAGTGTGC GCCTTGGGCC AGCGGAGCAG TCTGACGCAG ATGATGAAGA GGAGTCTGAA 
CAGCTGAGCT CTCAGCATAA AATTAAAGGT GGCAGAAAAA GACTTAACAG CCAAGCTTCA 
GAGAATGTTG AGAAGCGAAG AAGTATCAAT CTAAGTGTAT GTGAGAGCCA GATATCAAAA 
AGCCAGTCAG ATGAAGAGTC AACAAAGCTG AATACAGGGC AGATCTTTCC AAGTTCCAGT 
GGTCATCTTC CAAGCAGACA GAATGGTGCA CAACAGAGCA ATTCTTTGAT TATGAATATC 
CTTGGAGGTA CTGGTGCTCT GAGGAACATC TGGACTGATC ATCAAATTAG ACAAGCCCTC 
TTTCCTTCTA GACGCTCTAC ACAGGAAAAT GAGGAGGATG ATGATGACTA CCAGATGTTT 
CTGTTGTCTG AATCTGCAAC CAGGTTGGAA TCGTCATGGT CACAGGAAGA AAGAGTGGCA 
TCAAGCCGGC CATGTAGCTG GCACCTTGGT CTTATGGAAG AAGACACAAT ACCAAACCCC 
TCCTGCAAAA TTGTCAGGCG TGCAAGTAGT GTTGGAGAGA ATAAATCTTG TTCTGCAAGA 
CAACGTAGCC ACTATCATAA ATCGGGGCCT CATGAACATT TCAATCGTTC ACATGAGCGT 
CATCAACATT TTACTTGTCT TGGCTCTAAT GAGGACTTAA CCATTGATGA CATTGAACAT 
GTTTATGACA ATATTAGTTA TGAAGATCTG AAACAGATGG GGTTCACCAG GAGACATGAT 
TCTGATGCTC CTATAAATAA GGAAGGAGGG GATTCTCAAA GCCAAGAAGA CATTAGTATT 
AGAAGACTAA GAGAACAGTT TGATAGAACC AGGAACTCCC TAATCCTAAA TAAAGAGAAA 
AATCTAAAGA AAGGAATGTC CAATTCAAAT TCACAAGAAC TCAGAATAAT TGAGGAAAAC 
ATTTATGACT CTATTCAGCT ACCAGAACAA TCTCTGGAGC ATGTAAAGCA TAAATTGCAT 
TCTAAACGGA GCAATTTTTT GGCTCTGGGC ACTGATTTTC AGTCTTGTGA TCAGCTTGAT 
AGGTTTGTTT CCGAAGACAG CCTACAGTTC AGCGAAGATG AATCACTGGA TCATCGTGTA 
CCAGTGAAAG ATGGAGATTT CTGTTATTCC TCTTCAAAGG CTGGTTCAGT ATCACATAAG 
TCTGCAGCAG ATAAATTGTC AGAGGAAGTA GATGAAATCT GGAATGATTT GGAAAACTAC 
ATTAAGAAGA ATGAGGAGAA AAAAAGTGAA CGGCTTTTAG CATCATTCCC TGTAAACAAA 
GATGACGTTC TTGAAAGGCG CCATGTAACG TACATGCCTT GTTTCAGCAA AGATATGGAG 
TATTCATTGT CGTCATTGTG CTTGCCAGAA GATTTGCCTT CACAGAGGAC AATACTGAAT 
ACTTCACAAA TAAGTAATGA TAATAGTAGC TGTGGAAGCT CAAATAGTGG CTCATTACTA 
AGTCTTAAGA GGACTTCCAT TGTAAGTGAA ACAGGCTTTC TAGAATCACC CCTTTTTGCT 
TCAAACAGCA CTATTCCCTA TTCCGAAGAT ACATGTTCTT CTCAAACTTT AGAGAGCACT 
GATAAATCAA AAAGCAAGGT TTTTATGATG GCTCGCCAAT ATAGTCAAAA AATAAAAAAA 
GCCAACCAGC TTTTAAAGAT GAAAAGCCCA GAACAAGAAC AGCCCTTTTA CAAATCACAG 
AAAATGAAAC CGAAGGATCT TGCAGCTATT ATGGAAGAGA AGAAACAAGG AGGCTGTGCT 
ATTGGTGCAA GAATTGCCGA GTATTCCCAG CTTTATGACC AGATTATCTT TCGCGACTCC 
CCCATAAGGA TGCAAAAGGA AGCAAAGAAA GACTCCCACA AAGTAGCATC TCCAAGTTAC 
TCTCCATTAA GCCCAACTTG CCAGCAACCG TCCTCTCCAT CTAAATGGCA ACAGCGAGGT 
AAATGTCTCC AATCAAATAG CACTGGTTTA CAGTCTGTTA ATGGAAGTGG GGCAGAATTT 
TATACATGGC CGGATGTAAA GGATTTTAAA TCAAAAACGG ATTTATCAGA ACCTGGCTCT 
GGGTGCCCTC ACAGAAACAT TACAGCTGCT TGTTCAGTGC CATCTTTGTC AAAGTCATTA 
CAGGATGCAT CGCATGAGTC AAGGTGGAGT GGCGTAAGCC AGCAGCATAG TGAAAATGGA 
CAAATGGTTT GTGCTTCTAA TTCACCAGAA AGGATAGTGA ATAAAGAACC TCAGCTTTAT 
ATTCGATCAC AGTCTTCATC TAGTATGCTA CATAACAGGA CATCTGGCTC TCACAGACTG 
GCAACAAACT CACAGCGGTC AAAGCCCATC TTAAAACCTA CAAGGCCTAC AGATCTTGGT 
CATCTTGGAT ATGAAAGAAA ATCCCAAATT GAAGACGACA CCTCTGATCT TACCTTGCAA 
GATTCCCAGA AGGTTTTGGT CATGAACACA CTGTCCTCTC TTAATGCTCA AATAGCTACG 
CTCAACTATT ATGCTAACTT TAAGGATACT GATGGAGATG ACGATGATGA TGACTATGTA 
GAGATCAAGT CAGAAGATGA AGAGGATGAG TTAGAGACTG ATTCTCATCA GGCTCTTAAA 
ATGAGTGTAA AAGGAAAGCC AAATGTTACT CAGACTTACT GTAGTACAAA TCCAACCTAT 
TCCTTTAGCG TGCCCAGTAC TCCAGTCAAG TCCTACAACA GCAAGGAACT CTCTGATGCA 
CATATATTAG CCTATGACGA TGCAGACAAA CTAAATGACT ATCTCTGGAG ACCAACGCCT 
TCTAATCAGC AAAATATTGT TCAGTCACTT AGAGAAAAAT TTCAGTGTCT CAGCTCAAGT 
AGTTTTGCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekhg1 ( XM_018094253.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017949742.1 Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G1 (plekhg1), transcript variant X1, mRNA.

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CloneID	OXa44665
Clone ID Related Accession (Same CDS sequence)	XM_018094253.1 , XM_018094253.2
Accession Version	XM_018094253.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4692bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology domain-containing family G member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018094253.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGATA AAGCACCAGA GGGTACAGAA ACAGCATCCC AGAATGAAGA GGACTACCAT 
TTGCCTCTTT ATGGTCTTCC ATCTGTAAGA GAGCCTCAGA GTCCTAGGAC AGCAGAGGAA 
AATCTGCAAA GGACCAGCAG ACATTTCCGT TACTGTGGGA TCAAAAACAT GGAGATGTCA 
GACAGTGACC GGCCAGTCAG CTTTGGTTCA ACATCCTCAT CTGCCTCTTC CAGAGACAGT 
CACTGTTCAT TTGGCAGTCG GATGACCCTA GCATCAAACA GTCACTTGGG TTTGTTTCAC 
CAAGATAAGG AAGCTGGAGC CATAAAACTA GAACTGGTTC CTGCCCGGCA GATATCAAAT 
GATGACCTTC ATAGGATCTG CACCAAAGAA CAACCTCATG GAAGTCCAAG GGAAAAACCA 
TCTGATGAGC ATCAGAGCAC ACAGTGGAAA GCTGAAGCTA AAAGATCAAT TAAATGTTCA 
GTGACTGAAG CGGTAGAGAC AACCAGTCCT AAACTTATGT ATGTAGACAG AGTAGTTCAA 
GAAATTCTCC ATACTGAAAG GGCCTATGTG CAGGATCTGA AAAGCATTGT TGAGGATTAT 
CTCAATTGCA TTTCTGACCA ATCAAGACTT TCCCTAGGGA CAGAAGAACG GTCAGCTCTC 
TTTGGCAATA TATGCGATAT CTACCATTTC AATAGTGAAC TTCTTCAAGA ACTGGAAAAT 
TGTGATAATG ATCCTGTTGC AATAGCCGAA TGCTTTGTGT CAAAGAGTGA GGAATTCCAT 
ATATACACAC AGTATTGTAC TAATTATCCA AGATCTGTTG CTATATTGAC AGAGTGTATG 
AGAAACAAAA TACTGGCCAA ATTCTTTAGA GAACGACAGG AAGTCCTACA ACATTCTCTT 
CCCTTAGGAT CTTACCTCTT GAAGCCTGTC CAGAGAATCC TTAAGTACCA CTTGCTGTTG 
CATGAAATAT CCAATCATTT GGATAAAGAC ACTGAAGGCT ATGACATTGT GCTGGATGCC 
ATAGACACAA TGCAAAGGGT TGCATGGCAT ATAAATGATA TGAAGAGGAA ACATGAACAT 
GACATTCGGC TCCAGGAAAT CCAGAGTTTG CTCACTAATT GGAAAGGACC AGATCTGACA 
AGTTACGGGG AGCTAGTTTT GGAAGGAACA TTCCGTTTTC AGAGGGCCAA GAATGAACGC 
ACTTTGTTCC TGTTCGACCG TCTGCTTCTA ATCACAAAGA AAAGGGATGA CACATACACA 
TACAAGGCTC ATATCCTGTG TTGCAATCTC ATGCTAGTTG AAGTAATTCC AAAGGAGCCA 
CTTAGCTTCA GTGTCTTCCA TTATAAGAAC CCAAAGCTAC AGCACACAGT ACAGGCAAAG 
TCACAGCAAG ACAAGAGGCT CTGGATTTTG CATTTGAAGA GACTTATCCT TGAAAACCAC 
CCAGCGAAAA TCCCAGCAAA GGCTAAACAA GCAATTTTGG AAATGGATGC TATACATCAT 
CCCGGGTTCT CCTATACCCC AGAGGGAGAA CGGAAATCAC CTTTAAACTT GAAAGAAGAT 
CTCGCATCAT GCAGAGTCAG AAGAAAGTCA GAACCTTCGT CTAATCTACA CAAAGTCATG 
AAGCAAGATG GTTTAAGCCC AGAGATGCAA AAGAGAAGTA GCATACAGGG CAATTTGCTA 
TGCCAGGCTT CTAAACTCAG TGGAAGTGAT CTGCTTCGTG GGTCTGGGAT GAGAAAAGGT 
GGCTCAATGA ATCACAACAG CAATATGACC CGGTCTCAAG ATTTCTACAA CAAAGAAGGT 
CTAAGTGTGC GCCTTGGGCC AGCGGAGCAG TCTGACGCAG ATGATGAAGA GGAGTCTGAA 
CAGCTGAGCT CTCAGCATAA AATTAAAGGT GGCAGAAAAA GACTTAACAG CCAAGCTTCA 
GAGAATGTTG AGAAGCGAAG AAGTATCAAT CTAAGTGTAT GTGAGAGCCA GATATCAAAA 
AGCCAGTCAG ATGAAGAGTC AACAAAGCTG AATACAGGGC AGATCTTTCC AAGTTCCAGT 
GGTCATCTTC CAAGCAGACA GAATGGTGCA CAACAGAGCA ATTCTTTGAT TATGAATATC 
CTTGGAGGTA CTGGTGCTCT GAGGAACATC TGGACTGATC ATCAAATTAG ACAAGCCCTC 
TTTCCTTCTA GACGCTCTAC ACAGGAAAAT GAGGAGGATG ATGATGACTA CCAGATGTTT 
CTGTTGTCTG AATCTGCAAC CAGGTTGGAA TCGTCATGGT CACAGGAAGA AAGAGTGGCA 
TCAAGCCGGC CATGTAGCTG GCACCTTGGT CTTATGGAAG AAGACACAAT ACCAAACCCC 
TCCTGCAAAA TTGTCAGGCG TGCAAGTAGT GTTGGAGAGA ATAAATCTTG TTCTGCAAGA 
CAACGTAGCC ACTATCATAA ATCGGGGCCT CATGAACATT TCAATCGTTC ACATGAGCGT 
CATCAACATT TTACTTGTCT TGGCTCTAAT GAGGACTTAA CCATTGATGA CATTGAACAT 
GTTTATGACA ATATTAGTTA TGAAGATCTG AAACAGATGG GGTTCACCAG GAGACATGAT 
TCTGATGCTC CTATAAATAA GGAAGGAGGG GATTCTCAAA GCCAAGAAGA CATTAGTATT 
AGAAGACTAA GAGAACAGTT TGATAGAACC AGGAACTCCC TAATCCTAAA TAAAGAGAAA 
AATCTAAAGA AAGGAATGTC CAATTCAAAT TCACAAGAAC TCAGAATAAT TGAGGAAAAC 
ATTTATGACT CTATTCAGCT ACCAGAACAA TCTCTGGAGC ATGTAAAGCA TAAATTGCAT 
TCTAAACGGA GCAATTTTTT GGCTCTGGGC ACTGATTTTC AGTCTTGTGA TCAGCTTGAT 
AGGTTTGTTT CCGAAGACAG CCTACAGTTC AGCGAAGATG AATCACTGGA TCATCGTGTA 
CCAGTGAAAG ATGGAGATTT CTGTTATTCC TCTTCAAAGG CTGGTTCAGT ATCACATAAG 
TCTGCAGCAG ATAAATTGTC AGAGGAAGTA GATGAAATCT GGAATGATTT GGAAAACTAC 
ATTAAGAAGA ATGAGGAGAA AAAAAGTGAA CGGCTTTTAG CATCATTCCC TGTAAACAAA 
GATGACGTTC TTGAAAGGCG CCATGTAACG TACATGCCTT GTTTCAGCAA AGATATGGAG 
TATTCATTGT CGTCATTGTG CTTGCCAGAA GATTTGCCTT CACAGAGGAC AATACTGAAT 
ACTTCACAAA TAAGTAATGA TAATAGTAGC TGTGGAAGCT CAAATAGTGG CTCATTACTA 
AGTCTTAAGA GGACTTCCAT TGTAAGTGAA ACAGGCTTTC TAGAATCACC CCTTTTTGCT 
TCAAACAGCA CTATTCCCTA TTCCGAAGAT ACATGTTCTT CTCAAACTTT AGAGAGCACT 
GATAAATCAA AAAGCAAGGT TTTTATGATG GCTCGCCAAT ATAGTCAAAA AATAAAAAAA 
GCCAACCAGC TTTTAAAGAT GAAAAGCCCA GAACAAGAAC AGCCCTTTTA CAAATCACAG 
AAAATGAAAC CGAAGGATCT TGCAGCTATT ATGGAAGAGA AGAAACAAGG AGGCTGTGCT 
ATTGGTGCAA GAATTGCCGA GTATTCCCAG CTTTATGACC AGATTATCTT TCGCGACTCC 
CCCATAAGGA TGCAAAAGGA AGCAAAGAAA GACTCCCACA AAGTAGCATC TCCAAGTTAC 
TCTCCATTAA GCCCAACTTG CCAGCAACCG TCCTCTCCAT CTAAATGGCA ACAGCGAGGT 
AAATGTCTCC AATCAAATAG CACTGGTTTA CAGTCTGTTA ATGGAAGTGG GGCAGAATTT 
TATACATGGC CGGATGTAAA GGATTTTAAA TCAAAAACGG ATTTATCAGA ACCTGGCTCT 
GGGTGCCCTC ACAGAAACAT TACAGCTGCT TGTTCAGTGC CATCTTTGTC AAAGTCATTA 
CAGGATGCAT CGCATGAGTC AAGGTGGAGT GGCGTAAGCC AGCAGCATAG TGAAAATGGA 
CAAATGGTTT GTGCTTCTAA TTCACCAGAA AGGATAGTGA ATAAAGAACC TCAGCTTTAT 
ATTCGATCAC AGTCTTCATC TAGTATGCTA CATAACAGGA CATCTGGCTC TCACAGACTG 
GCAACAAACT CACAGCGGTC AAAGCCCATC TTAAAACCTA CAAGGCCTAC AGATCTTGGT 
CATCTTGGAT ATGAAAGAAA ATCCCAAATT GAAGACGACA CCTCTGATCT TACCTTGCAA 
GATTCCCAGA AGGTTTTGGT CATGAACACA CTGTCCTCTC TTAATGCTCA AATAGCTACG 
CTCAACTATT ATGCTAACTT TAAGGATACT GATGGAGATG ACGATGATGA TGACTATGTA 
GAGATCAAGT CAGAAGATGA AGAGGATGAG TTAGAGACTG ATTCTCATCA GGCTCTTAAA 
ATGAGTGTAA AAGGAAAGCC AAATGTTACT CAGACTTACT GTAGTACAAA TCCAACCTAT 
TCCTTTAGCG TGCCCAGTAC TCCAGTCAAG TCCTACAACA GCAAGGAACT CTCTGATGCA 
CATATATTAG CCTATGACGA TGCAGACAAA CTAAATGACT ATCTCTGGAG ACCAACGCCT 
TCTAATCAGC AAAATATTGT TCAGTCACTT AGAGAAAAAT TTCAGTGTCT CAGCTCAAGT 
AGTTTTGCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017949742.1
CDS	257..4948
Translation	MPDKAPEGTETASQNEEDYHLPLYGLPSVREPQSPRTAEENLQRTSRHFRYCGIKNMEMSDSDRPVSFGSTSSSASSRDSHCSFGSRMTLASNSHLGLFHQDKEAGAIKLELVPARQISNDDLHRICTKEQPHGSPREKPSDEHQSTQWKAEAKRSIKCSVTEAVETTSPKLMYVDRVVQEILHTERAYVQDLKSIVEDYLNCISDQSRLSLGTEERSALFGNICDIYHFNSELLQELENCDNDPVAIAECFVSKSEEFHIYTQYCTNYPRSVAILTECMRNKILAKFFRERQEVLQHSLPLGSYLLKPVQRILKYHLLLHEISNHLDKDTEGYDIVLDAIDTMQRVAWHINDMKRKHEHDIRLQEIQSLLTNWKGPDLTSYGELVLEGTFRFQRAKNERTLFLFDRLLLITKKRDDTYTYKAHILCCNLMLVEVIPKEPLSFSVFHYKNPKLQHTVQAKSQQDKRLWILHLKRLILENHPAKIPAKAKQAILEMDAIHHPGFSYTPEGERKSPLNLKEDLASCRVRRKSEPSSNLHKVMKQDGLSPEMQKRSSIQGNLLCQASKLSGSDLLRGSGMRKGGSMNHNSNMTRSQDFYNKEGLSVRLGPAEQSDADDEEESEQLSSQHKIKGGRKRLNSQASENVEKRRSINLSVCESQISKSQSDEESTKLNTGQIFPSSSGHLPSRQNGAQQSNSLIMNILGGTGALRNIWTDHQIRQALFPSRRSTQENEEDDDDYQMFLLSESATRLESSWSQEERVASSRPCSWHLGLMEEDTIPNPSCKIVRRASSVGENKSCSARQRSHYHKSGPHEHFNRSHERHQHFTCLGSNEDLTIDDIEHVYDNISYEDLKQMGFTRRHDSDAPINKEGGDSQSQEDISIRRLREQFDRTRNSLILNKEKNLKKGMSNSNSQELRIIEENIYDSIQLPEQSLEHVKHKLHSKRSNFLALGTDFQSCDQLDRFVSEDSLQFSEDESLDHRVPVKDGDFCYSSSKAGSVSHKSAADKLSEEVDEIWNDLENYIKKNEEKKSERLLASFPVNKDDVLERRHVTYMPCFSKDMEYSLSSLCLPEDLPSQRTILNTSQISNDNSSCGSSNSGSLLSLKRTSIVSETGFLESPLFASNSTIPYSEDTCSSQTLESTDKSKSKVFMMARQYSQKIKKANQLLKMKSPEQEQPFYKSQKMKPKDLAAIMEEKKQGGCAIGARIAEYSQLYDQIIFRDSPIRMQKEAKKDSHKVASPSYSPLSPTCQQPSSPSKWQQRGKCLQSNSTGLQSVNGSGAEFYTWPDVKDFKSKTDLSEPGSGCPHRNITAACSVPSLSKSLQDASHESRWSGVSQQHSENGQMVCASNSPERIVNKEPQLYIRSQSSSSMLHNRTSGSHRLATNSQRSKPILKPTRPTDLGHLGYERKSQIEDDTSDLTLQDSQKVLVMNTLSSLNAQIATLNYYANFKDTDGDDDDDDYVEIKSEDEEDELETDSHQALKMSVKGKPNVTQTYCSTNPTYSFSVPSTPVKSYNSKELSDAHILAYDDADKLNDYLWRPTPSNQQNIVQSLREKFQCLSSSSFA

Target ORF information:

RefSeq Version	XM_018094253.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G1 (plekhg1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018094253.2

ORF Insert Sequence:

ATGCCAGATA AAGCACCAGA GGGTACAGAA ACAGCATCCC AGAATGAAGA GGACTACCAT 
TTGCCTCTTT ATGGTCTTCC ATCTGTAAGA GAGCCTCAGA GTCCTAGGAC AGCAGAGGAA 
AATCTGCAAA GGACCAGCAG ACATTTCCGT TACTGTGGGA TCAAAAACAT GGAGATGTCA 
GACAGTGACC GGCCAGTCAG CTTTGGTTCA ACATCCTCAT CTGCCTCTTC CAGAGACAGT 
CACTGTTCAT TTGGCAGTCG GATGACCCTA GCATCAAACA GTCACTTGGG TTTGTTTCAC 
CAAGATAAGG AAGCTGGAGC CATAAAACTA GAACTGGTTC CTGCCCGGCA GATATCAAAT 
GATGACCTTC ATAGGATCTG CACCAAAGAA CAACCTCATG GAAGTCCAAG GGAAAAACCA 
TCTGATGAGC ATCAGAGCAC ACAGTGGAAA GCTGAAGCTA AAAGATCAAT TAAATGTTCA 
GTGACTGAAG CGGTAGAGAC AACCAGTCCT AAACTTATGT ATGTAGACAG AGTAGTTCAA 
GAAATTCTCC ATACTGAAAG GGCCTATGTG CAGGATCTGA AAAGCATTGT TGAGGATTAT 
CTCAATTGCA TTTCTGACCA ATCAAGACTT TCCCTAGGGA CAGAAGAACG GTCAGCTCTC 
TTTGGCAATA TATGCGATAT CTACCATTTC AATAGTGAAC TTCTTCAAGA ACTGGAAAAT 
TGTGATAATG ATCCTGTTGC AATAGCCGAA TGCTTTGTGT CAAAGAGTGA GGAATTCCAT 
ATATACACAC AGTATTGTAC TAATTATCCA AGATCTGTTG CTATATTGAC AGAGTGTATG 
AGAAACAAAA TACTGGCCAA ATTCTTTAGA GAACGACAGG AAGTCCTACA ACATTCTCTT 
CCCTTAGGAT CTTACCTCTT GAAGCCTGTC CAGAGAATCC TTAAGTACCA CTTGCTGTTG 
CATGAAATAT CCAATCATTT GGATAAAGAC ACTGAAGGCT ATGACATTGT GCTGGATGCC 
ATAGACACAA TGCAAAGGGT TGCATGGCAT ATAAATGATA TGAAGAGGAA ACATGAACAT 
GACATTCGGC TCCAGGAAAT CCAGAGTTTG CTCACTAATT GGAAAGGACC AGATCTGACA 
AGTTACGGGG AGCTAGTTTT GGAAGGAACA TTCCGTTTTC AGAGGGCCAA GAATGAACGC 
ACTTTGTTCC TGTTCGACCG TCTGCTTCTA ATCACAAAGA AAAGGGATGA CACATACACA 
TACAAGGCTC ATATCCTGTG TTGCAATCTC ATGCTAGTTG AAGTAATTCC AAAGGAGCCA 
CTTAGCTTCA GTGTCTTCCA TTATAAGAAC CCAAAGCTAC AGCACACAGT ACAGGCAAAG 
TCACAGCAAG ACAAGAGGCT CTGGATTTTG CATTTGAAGA GACTTATCCT TGAAAACCAC 
CCAGCGAAAA TCCCAGCAAA GGCTAAACAA GCAATTTTGG AAATGGATGC TATACATCAT 
CCCGGGTTCT CCTATACCCC AGAGGGAGAA CGGAAATCAC CTTTAAACTT GAAAGAAGAT 
CTCGCATCAT GCAGAGTCAG AAGAAAGTCA GAACCTTCGT CTAATCTACA CAAAGTCATG 
AAGCAAGATG GTTTAAGCCC AGAGATGCAA AAGAGAAGTA GCATACAGGG CAATTTGCTA 
TGCCAGGCTT CTAAACTCAG TGGAAGTGAT CTGCTTCGTG GGTCTGGGAT GAGAAAAGGT 
GGCTCAATGA ATCACAACAG CAATATGACC CGGTCTCAAG ATTTCTACAA CAAAGAAGGT 
CTAAGTGTGC GCCTTGGGCC AGCGGAGCAG TCTGACGCAG ATGATGAAGA GGAGTCTGAA 
CAGCTGAGCT CTCAGCATAA AATTAAAGGT GGCAGAAAAA GACTTAACAG CCAAGCTTCA 
GAGAATGTTG AGAAGCGAAG AAGTATCAAT CTAAGTGTAT GTGAGAGCCA GATATCAAAA 
AGCCAGTCAG ATGAAGAGTC AACAAAGCTG AATACAGGGC AGATCTTTCC AAGTTCCAGT 
GGTCATCTTC CAAGCAGACA GAATGGTGCA CAACAGAGCA ATTCTTTGAT TATGAATATC 
CTTGGAGGTA CTGGTGCTCT GAGGAACATC TGGACTGATC ATCAAATTAG ACAAGCCCTC 
TTTCCTTCTA GACGCTCTAC ACAGGAAAAT GAGGAGGATG ATGATGACTA CCAGATGTTT 
CTGTTGTCTG AATCTGCAAC CAGGTTGGAA TCGTCATGGT CACAGGAAGA AAGAGTGGCA 
TCAAGCCGGC CATGTAGCTG GCACCTTGGT CTTATGGAAG AAGACACAAT ACCAAACCCC 
TCCTGCAAAA TTGTCAGGCG TGCAAGTAGT GTTGGAGAGA ATAAATCTTG TTCTGCAAGA 
CAACGTAGCC ACTATCATAA ATCGGGGCCT CATGAACATT TCAATCGTTC ACATGAGCGT 
CATCAACATT TTACTTGTCT TGGCTCTAAT GAGGACTTAA CCATTGATGA CATTGAACAT 
GTTTATGACA ATATTAGTTA TGAAGATCTG AAACAGATGG GGTTCACCAG GAGACATGAT 
TCTGATGCTC CTATAAATAA GGAAGGAGGG GATTCTCAAA GCCAAGAAGA CATTAGTATT 
AGAAGACTAA GAGAACAGTT TGATAGAACC AGGAACTCCC TAATCCTAAA TAAAGAGAAA 
AATCTAAAGA AAGGAATGTC CAATTCAAAT TCACAAGAAC TCAGAATAAT TGAGGAAAAC 
ATTTATGACT CTATTCAGCT ACCAGAACAA TCTCTGGAGC ATGTAAAGCA TAAATTGCAT 
TCTAAACGGA GCAATTTTTT GGCTCTGGGC ACTGATTTTC AGTCTTGTGA TCAGCTTGAT 
AGGTTTGTTT CCGAAGACAG CCTACAGTTC AGCGAAGATG AATCACTGGA TCATCGTGTA 
CCAGTGAAAG ATGGAGATTT CTGTTATTCC TCTTCAAAGG CTGGTTCAGT ATCACATAAG 
TCTGCAGCAG ATAAATTGTC AGAGGAAGTA GATGAAATCT GGAATGATTT GGAAAACTAC 
ATTAAGAAGA ATGAGGAGAA AAAAAGTGAA CGGCTTTTAG CATCATTCCC TGTAAACAAA 
GATGACGTTC TTGAAAGGCG CCATGTAACG TACATGCCTT GTTTCAGCAA AGATATGGAG 
TATTCATTGT CGTCATTGTG CTTGCCAGAA GATTTGCCTT CACAGAGGAC AATACTGAAT 
ACTTCACAAA TAAGTAATGA TAATAGTAGC TGTGGAAGCT CAAATAGTGG CTCATTACTA 
AGTCTTAAGA GGACTTCCAT TGTAAGTGAA ACAGGCTTTC TAGAATCACC CCTTTTTGCT 
TCAAACAGCA CTATTCCCTA TTCCGAAGAT ACATGTTCTT CTCAAACTTT AGAGAGCACT 
GATAAATCAA AAAGCAAGGT TTTTATGATG GCTCGCCAAT ATAGTCAAAA AATAAAAAAA 
GCCAACCAGC TTTTAAAGAT GAAAAGCCCA GAACAAGAAC AGCCCTTTTA CAAATCACAG 
AAAATGAAAC CGAAGGATCT TGCAGCTATT ATGGAAGAGA AGAAACAAGG AGGCTGTGCT 
ATTGGTGCAA GAATTGCCGA GTATTCCCAG CTTTATGACC AGATTATCTT TCGCGACTCC 
CCCATAAGGA TGCAAAAGGA AGCAAAGAAA GACTCCCACA AAGTAGCATC TCCAAGTTAC 
TCTCCATTAA GCCCAACTTG CCAGCAACCG TCCTCTCCAT CTAAATGGCA ACAGCGAGGT 
AAATGTCTCC AATCAAATAG CACTGGTTTA CAGTCTGTTA ATGGAAGTGG GGCAGAATTT 
TATACATGGC CGGATGTAAA GGATTTTAAA TCAAAAACGG ATTTATCAGA ACCTGGCTCT 
GGGTGCCCTC ACAGAAACAT TACAGCTGCT TGTTCAGTGC CATCTTTGTC AAAGTCATTA 
CAGGATGCAT CGCATGAGTC AAGGTGGAGT GGCGTAAGCC AGCAGCATAG TGAAAATGGA 
CAAATGGTTT GTGCTTCTAA TTCACCAGAA AGGATAGTGA ATAAAGAACC TCAGCTTTAT 
ATTCGATCAC AGTCTTCATC TAGTATGCTA CATAACAGGA CATCTGGCTC TCACAGACTG 
GCAACAAACT CACAGCGGTC AAAGCCCATC TTAAAACCTA CAAGGCCTAC AGATCTTGGT 
CATCTTGGAT ATGAAAGAAA ATCCCAAATT GAAGACGACA CCTCTGATCT TACCTTGCAA 
GATTCCCAGA AGGTTTTGGT CATGAACACA CTGTCCTCTC TTAATGCTCA AATAGCTACG 
CTCAACTATT ATGCTAACTT TAAGGATACT GATGGAGATG ACGATGATGA TGACTATGTA 
GAGATCAAGT CAGAAGATGA AGAGGATGAG TTAGAGACTG ATTCTCATCA GGCTCTTAAA 
ATGAGTGTAA AAGGAAAGCC AAATGTTACT CAGACTTACT GTAGTACAAA TCCAACCTAT 
TCCTTTAGCG TGCCCAGTAC TCCAGTCAAG TCCTACAACA GCAAGGAACT CTCTGATGCA 
CATATATTAG CCTATGACGA TGCAGACAAA CTAAATGACT ATCTCTGGAG ACCAACGCCT 
TCTAATCAGC AAAATATTGT TCAGTCACTT AGAGAAAAAT TTCAGTGTCT CAGCTCAAGT 
AGTTTTGCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekhg1 ( XM_018094254.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017949743.1 Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G1 (plekhg1), transcript variant X3, mRNA.

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CloneID	OXa44666
Clone ID Related Accession (Same CDS sequence)	XM_018094254.1 , XM_018094254.2
Accession Version	XM_018094254.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4596bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology domain-containing family G member 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGATA AAGCACCAGA GGGTACAGAA ACAGCATCCC AGAATGAAGA GGACTACCAT 
TTGCCTCTTT ATGGTCTTCC ATCTGTAAGA GAGCCTCAGA GTCCTAGGAC AGCAGAGGAA 
AATCTGCAAA GGACCAGCAG ACATTTCCGT TACTGTGGGA TCAAAAACAT GGAGATGTCA 
GACAGTGACC GGCCAGTCAG CTTTGGTTCA ACATCCTCAT CTGCCTCTTC CAGAGACAGT 
CACTGTTCAT TTGGCAGTCG GATGACCCTA GCATCAAACA GTCACTTGGG TTTGTTTCAC 
CAAGATAAGG AAGCTGGAGC CATAAAACTA GAACTGGTTC CTGCCCGGCA GATATCAAAT 
GATGACCTTC ATAGGATCTG CACCAAAGAA CAACCTCATG GAAGTCCAAG GGAAAAACCA 
TCTGATGAGC ATCAGAGCAC ACAGTGGAAA GCTGAAGCTA AAAGATCAAT TAAATGTTCA 
GTGACTGAAG CGGTAGAGAC AACCAGTCCT AAACTTATGT ATGTAGACAG AGTAGTTCAA 
GAAATTCTCC ATACTGAAAG GGCCTATGTG CAGGATCTGA AAAGCATTGT TGAGGATTAT 
CTCAATTGCA TTTCTGACCA ATCAAGACTT TCCCTAGGGA CAGAAGAACG GTCAGCTCTC 
TTTGGCAATA TATGCGATAT CTACCATTTC AATAGTGAAC TTCTTCAAGA ACTGGAAAAT 
TGTGATAATG ATCCTGTTGC AATAGCCGAA TGCTTTGTGT CAAAGAGTGA GGAATTCCAT 
ATATACACAC AGTATTGTAC TAATTATCCA AGATCTGTTG CTATATTGAC AGAGTGTATG 
AGAAACAAAA TACTGGCCAA ATTCTTTAGA GAACGACAGG AAGTCCTACA ACATTCTCTT 
CCCTTAGGAT CTTACCTCTT GAAGCCTGTC CAGAGAATCC TTAAGTACCA CTTGCTGTTG 
CATGAAATAT CCAATCATTT GGATAAAGAC ACTGAAGGCT ATGACATTGT GCTGGATGCC 
ATAGACACAA TGCAAAGGGT TGCATGGCAT ATAAATGATA TGAAGAGGAA ACATGAACAT 
GACATTCGGC TCCAGGAAAT CCAGAGTTTG CTCACTAATT GGAAAGGACC AGATCTGACA 
AGTTACGGGG AGCTAGTTTT GGAAGGAACA TTCCGTTTTC AGAGGGCCAA GAATGAACGC 
ACTTTGTTCC TGTTCGACCG TCTGCTTCTA ATCACAAAGA AAAGGGATGA CACATACACA 
TACAAGGCTC ATATCCTGTG TTGCAATCTC ATGCTAGTTG AAGTAATTCC AAAGGAGCCA 
CTTAGCTTCA GTGTCTTCCA TTATAAGAAC CCAAAGCTAC AGCACACAGT ACAGGCAAAG 
TCACAGCAAG ACAAGAGGCT CTGGATTTTG CATTTGAAGA GACTTATCCT TGAAAACCAC 
CCAGCGAAAA TCCCAGCAAA GGCTAAACAA GCAATTTTGG AAATGGATGC TATACAACCT 
TCGTCTAATC TACACAAAGT CATGAAGCAA GATGGTTTAA GCCCAGAGAT GCAAAAGAGA 
AGTAGCATAC AGGGCAATTT GCTATGCCAG GCTTCTAAAC TCAGTGGAAG TGATCTGCTT 
CGTGGGTCTG GGATGAGAAA AGGTGGCTCA ATGAATCACA ACAGCAATAT GACCCGGTCT 
CAAGATTTCT ACAACAAAGA AGGTCTAAGT GTGCGCCTTG GGCCAGCGGA GCAGTCTGAC 
GCAGATGATG AAGAGGAGTC TGAACAGCTG AGCTCTCAGC ATAAAATTAA AGGTGGCAGA 
AAAAGACTTA ACAGCCAAGC TTCAGAGAAT GTTGAGAAGC GAAGAAGTAT CAATCTAAGT 
GTATGTGAGA GCCAGATATC AAAAAGCCAG TCAGATGAAG AGTCAACAAA GCTGAATACA 
GGGCAGATCT TTCCAAGTTC CAGTGGTCAT CTTCCAAGCA GACAGAATGG TGCACAACAG 
AGCAATTCTT TGATTATGAA TATCCTTGGA GGTACTGGTG CTCTGAGGAA CATCTGGACT 
GATCATCAAA TTAGACAAGC CCTCTTTCCT TCTAGACGCT CTACACAGGA AAATGAGGAG 
GATGATGATG ACTACCAGAT GTTTCTGTTG TCTGAATCTG CAACCAGGTT GGAATCGTCA 
TGGTCACAGG AAGAAAGAGT GGCATCAAGC CGGCCATGTA GCTGGCACCT TGGTCTTATG 
GAAGAAGACA CAATACCAAA CCCCTCCTGC AAAATTGTCA GGCGTGCAAG TAGTGTTGGA 
GAGAATAAAT CTTGTTCTGC AAGACAACGT AGCCACTATC ATAAATCGGG GCCTCATGAA 
CATTTCAATC GTTCACATGA GCGTCATCAA CATTTTACTT GTCTTGGCTC TAATGAGGAC 
TTAACCATTG ATGACATTGA ACATGTTTAT GACAATATTA GTTATGAAGA TCTGAAACAG 
ATGGGGTTCA CCAGGAGACA TGATTCTGAT GCTCCTATAA ATAAGGAAGG AGGGGATTCT 
CAAAGCCAAG AAGACATTAG TATTAGAAGA CTAAGAGAAC AGTTTGATAG AACCAGGAAC 
TCCCTAATCC TAAATAAAGA GAAAAATCTA AAGAAAGGAA TGTCCAATTC AAATTCACAA 
GAACTCAGAA TAATTGAGGA AAACATTTAT GACTCTATTC AGCTACCAGA ACAATCTCTG 
GAGCATGTAA AGCATAAATT GCATTCTAAA CGGAGCAATT TTTTGGCTCT GGGCACTGAT 
TTTCAGTCTT GTGATCAGCT TGATAGGTTT GTTTCCGAAG ACAGCCTACA GTTCAGCGAA 
GATGAATCAC TGGATCATCG TGTACCAGTG AAAGATGGAG ATTTCTGTTA TTCCTCTTCA 
AAGGCTGGTT CAGTATCACA TAAGTCTGCA GCAGATAAAT TGTCAGAGGA AGTAGATGAA 
ATCTGGAATG ATTTGGAAAA CTACATTAAG AAGAATGAGG AGAAAAAAAG TGAACGGCTT 
TTAGCATCAT TCCCTGTAAA CAAAGATGAC GTTCTTGAAA GGCGCCATGT AACGTACATG 
CCTTGTTTCA GCAAAGATAT GGAGTATTCA TTGTCGTCAT TGTGCTTGCC AGAAGATTTG 
CCTTCACAGA GGACAATACT GAATACTTCA CAAATAAGTA ATGATAATAG TAGCTGTGGA 
AGCTCAAATA GTGGCTCATT ACTAAGTCTT AAGAGGACTT CCATTGTAAG TGAAACAGGC 
TTTCTAGAAT CACCCCTTTT TGCTTCAAAC AGCACTATTC CCTATTCCGA AGATACATGT 
TCTTCTCAAA CTTTAGAGAG CACTGATAAA TCAAAAAGCA AGGTTTTTAT GATGGCTCGC 
CAATATAGTC AAAAAATAAA AAAAGCCAAC CAGCTTTTAA AGATGAAAAG CCCAGAACAA 
GAACAGCCCT TTTACAAATC ACAGAAAATG AAACCGAAGG ATCTTGCAGC TATTATGGAA 
GAGAAGAAAC AAGGAGGCTG TGCTATTGGT GCAAGAATTG CCGAGTATTC CCAGCTTTAT 
GACCAGATTA TCTTTCGCGA CTCCCCCATA AGGATGCAAA AGGAAGCAAA GAAAGACTCC 
CACAAAGTAG CATCTCCAAG TTACTCTCCA TTAAGCCCAA CTTGCCAGCA ACCGTCCTCT 
CCATCTAAAT GGCAACAGCG AGGTAAATGT CTCCAATCAA ATAGCACTGG TTTACAGTCT 
GTTAATGGAA GTGGGGCAGA ATTTTATACA TGGCCGGATG TAAAGGATTT TAAATCAAAA 
ACGGATTTAT CAGAACCTGG CTCTGGGTGC CCTCACAGAA ACATTACAGC TGCTTGTTCA 
GTGCCATCTT TGTCAAAGTC ATTACAGGAT GCATCGCATG AGTCAAGGTG GAGTGGCGTA 
AGCCAGCAGC ATAGTGAAAA TGGACAAATG GTTTGTGCTT CTAATTCACC AGAAAGGATA 
GTGAATAAAG AACCTCAGCT TTATATTCGA TCACAGTCTT CATCTAGTAT GCTACATAAC 
AGGACATCTG GCTCTCACAG ACTGGCAACA AACTCACAGC GGTCAAAGCC CATCTTAAAA 
CCTACAAGGC CTACAGATCT TGGTCATCTT GGATATGAAA GAAAATCCCA AATTGAAGAC 
GACACCTCTG ATCTTACCTT GCAAGATTCC CAGAAGGTTT TGGTCATGAA CACACTGTCC 
TCTCTTAATG CTCAAATAGC TACGCTCAAC TATTATGCTA ACTTTAAGGA TACTGATGGA 
GATGACGATG ATGATGACTA TGTAGAGATC AAGTCAGAAG ATGAAGAGGA TGAGTTAGAG 
ACTGATTCTC ATCAGGCTCT TAAAATGAGT GTAAAAGGAA AGCCAAATGT TACTCAGACT 
TACTGTAGTA CAAATCCAAC CTATTCCTTT AGCGTGCCCA GTACTCCAGT CAAGTCCTAC 
AACAGCAAGG AACTCTCTGA TGCACATATA TTAGCCTATG ACGATGCAGA CAAACTAAAT 
GACTATCTCT GGAGACCAAC GCCTTCTAAT CAGCAAAATA TTGTTCAGTC ACTTAGAGAA 
AAATTTCAGT GTCTCAGCTC AAGTAGTTTT GCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017949743.1
CDS	235..4830
Translation	MPDKAPEGTETASQNEEDYHLPLYGLPSVREPQSPRTAEENLQRTSRHFRYCGIKNMEMSDSDRPVSFGSTSSSASSRDSHCSFGSRMTLASNSHLGLFHQDKEAGAIKLELVPARQISNDDLHRICTKEQPHGSPREKPSDEHQSTQWKAEAKRSIKCSVTEAVETTSPKLMYVDRVVQEILHTERAYVQDLKSIVEDYLNCISDQSRLSLGTEERSALFGNICDIYHFNSELLQELENCDNDPVAIAECFVSKSEEFHIYTQYCTNYPRSVAILTECMRNKILAKFFRERQEVLQHSLPLGSYLLKPVQRILKYHLLLHEISNHLDKDTEGYDIVLDAIDTMQRVAWHINDMKRKHEHDIRLQEIQSLLTNWKGPDLTSYGELVLEGTFRFQRAKNERTLFLFDRLLLITKKRDDTYTYKAHILCCNLMLVEVIPKEPLSFSVFHYKNPKLQHTVQAKSQQDKRLWILHLKRLILENHPAKIPAKAKQAILEMDAIQPSSNLHKVMKQDGLSPEMQKRSSIQGNLLCQASKLSGSDLLRGSGMRKGGSMNHNSNMTRSQDFYNKEGLSVRLGPAEQSDADDEEESEQLSSQHKIKGGRKRLNSQASENVEKRRSINLSVCESQISKSQSDEESTKLNTGQIFPSSSGHLPSRQNGAQQSNSLIMNILGGTGALRNIWTDHQIRQALFPSRRSTQENEEDDDDYQMFLLSESATRLESSWSQEERVASSRPCSWHLGLMEEDTIPNPSCKIVRRASSVGENKSCSARQRSHYHKSGPHEHFNRSHERHQHFTCLGSNEDLTIDDIEHVYDNISYEDLKQMGFTRRHDSDAPINKEGGDSQSQEDISIRRLREQFDRTRNSLILNKEKNLKKGMSNSNSQELRIIEENIYDSIQLPEQSLEHVKHKLHSKRSNFLALGTDFQSCDQLDRFVSEDSLQFSEDESLDHRVPVKDGDFCYSSSKAGSVSHKSAADKLSEEVDEIWNDLENYIKKNEEKKSERLLASFPVNKDDVLERRHVTYMPCFSKDMEYSLSSLCLPEDLPSQRTILNTSQISNDNSSCGSSNSGSLLSLKRTSIVSETGFLESPLFASNSTIPYSEDTCSSQTLESTDKSKSKVFMMARQYSQKIKKANQLLKMKSPEQEQPFYKSQKMKPKDLAAIMEEKKQGGCAIGARIAEYSQLYDQIIFRDSPIRMQKEAKKDSHKVASPSYSPLSPTCQQPSSPSKWQQRGKCLQSNSTGLQSVNGSGAEFYTWPDVKDFKSKTDLSEPGSGCPHRNITAACSVPSLSKSLQDASHESRWSGVSQQHSENGQMVCASNSPERIVNKEPQLYIRSQSSSSMLHNRTSGSHRLATNSQRSKPILKPTRPTDLGHLGYERKSQIEDDTSDLTLQDSQKVLVMNTLSSLNAQIATLNYYANFKDTDGDDDDDDYVEIKSEDEEDELETDSHQALKMSVKGKPNVTQTYCSTNPTYSFSVPSTPVKSYNSKELSDAHILAYDDADKLNDYLWRPTPSNQQNIVQSLREKFQCLSSSSFA

Target ORF information:

RefSeq Version	XM_018094254.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G1 (plekhg1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018094254.1

ORF Insert Sequence:

ATGCCAGATA AAGCACCAGA GGGTACAGAA ACAGCATCCC AGAATGAAGA GGACTACCAT 
TTGCCTCTTT ATGGTCTTCC ATCTGTAAGA GAGCCTCAGA GTCCTAGGAC AGCAGAGGAA 
AATCTGCAAA GGACCAGCAG ACATTTCCGT TACTGTGGGA TCAAAAACAT GGAGATGTCA 
GACAGTGACC GGCCAGTCAG CTTTGGTTCA ACATCCTCAT CTGCCTCTTC CAGAGACAGT 
CACTGTTCAT TTGGCAGTCG GATGACCCTA GCATCAAACA GTCACTTGGG TTTGTTTCAC 
CAAGATAAGG AAGCTGGAGC CATAAAACTA GAACTGGTTC CTGCCCGGCA GATATCAAAT 
GATGACCTTC ATAGGATCTG CACCAAAGAA CAACCTCATG GAAGTCCAAG GGAAAAACCA 
TCTGATGAGC ATCAGAGCAC ACAGTGGAAA GCTGAAGCTA AAAGATCAAT TAAATGTTCA 
GTGACTGAAG CGGTAGAGAC AACCAGTCCT AAACTTATGT ATGTAGACAG AGTAGTTCAA 
GAAATTCTCC ATACTGAAAG GGCCTATGTG CAGGATCTGA AAAGCATTGT TGAGGATTAT 
CTCAATTGCA TTTCTGACCA ATCAAGACTT TCCCTAGGGA CAGAAGAACG GTCAGCTCTC 
TTTGGCAATA TATGCGATAT CTACCATTTC AATAGTGAAC TTCTTCAAGA ACTGGAAAAT 
TGTGATAATG ATCCTGTTGC AATAGCCGAA TGCTTTGTGT CAAAGAGTGA GGAATTCCAT 
ATATACACAC AGTATTGTAC TAATTATCCA AGATCTGTTG CTATATTGAC AGAGTGTATG 
AGAAACAAAA TACTGGCCAA ATTCTTTAGA GAACGACAGG AAGTCCTACA ACATTCTCTT 
CCCTTAGGAT CTTACCTCTT GAAGCCTGTC CAGAGAATCC TTAAGTACCA CTTGCTGTTG 
CATGAAATAT CCAATCATTT GGATAAAGAC ACTGAAGGCT ATGACATTGT GCTGGATGCC 
ATAGACACAA TGCAAAGGGT TGCATGGCAT ATAAATGATA TGAAGAGGAA ACATGAACAT 
GACATTCGGC TCCAGGAAAT CCAGAGTTTG CTCACTAATT GGAAAGGACC AGATCTGACA 
AGTTACGGGG AGCTAGTTTT GGAAGGAACA TTCCGTTTTC AGAGGGCCAA GAATGAACGC 
ACTTTGTTCC TGTTCGACCG TCTGCTTCTA ATCACAAAGA AAAGGGATGA CACATACACA 
TACAAGGCTC ATATCCTGTG TTGCAATCTC ATGCTAGTTG AAGTAATTCC AAAGGAGCCA 
CTTAGCTTCA GTGTCTTCCA TTATAAGAAC CCAAAGCTAC AGCACACAGT ACAGGCAAAG 
TCACAGCAAG ACAAGAGGCT CTGGATTTTG CATTTGAAGA GACTTATCCT TGAAAACCAC 
CCAGCGAAAA TCCCAGCAAA GGCTAAACAA GCAATTTTGG AAATGGATGC TATACAACCT 
TCGTCTAATC TACACAAAGT CATGAAGCAA GATGGTTTAA GCCCAGAGAT GCAAAAGAGA 
AGTAGCATAC AGGGCAATTT GCTATGCCAG GCTTCTAAAC TCAGTGGAAG TGATCTGCTT 
CGTGGGTCTG GGATGAGAAA AGGTGGCTCA ATGAATCACA ACAGCAATAT GACCCGGTCT 
CAAGATTTCT ACAACAAAGA AGGTCTAAGT GTGCGCCTTG GGCCAGCGGA GCAGTCTGAC 
GCAGATGATG AAGAGGAGTC TGAACAGCTG AGCTCTCAGC ATAAAATTAA AGGTGGCAGA 
AAAAGACTTA ACAGCCAAGC TTCAGAGAAT GTTGAGAAGC GAAGAAGTAT CAATCTAAGT 
GTATGTGAGA GCCAGATATC AAAAAGCCAG TCAGATGAAG AGTCAACAAA GCTGAATACA 
GGGCAGATCT TTCCAAGTTC CAGTGGTCAT CTTCCAAGCA GACAGAATGG TGCACAACAG 
AGCAATTCTT TGATTATGAA TATCCTTGGA GGTACTGGTG CTCTGAGGAA CATCTGGACT 
GATCATCAAA TTAGACAAGC CCTCTTTCCT TCTAGACGCT CTACACAGGA AAATGAGGAG 
GATGATGATG ACTACCAGAT GTTTCTGTTG TCTGAATCTG CAACCAGGTT GGAATCGTCA 
TGGTCACAGG AAGAAAGAGT GGCATCAAGC CGGCCATGTA GCTGGCACCT TGGTCTTATG 
GAAGAAGACA CAATACCAAA CCCCTCCTGC AAAATTGTCA GGCGTGCAAG TAGTGTTGGA 
GAGAATAAAT CTTGTTCTGC AAGACAACGT AGCCACTATC ATAAATCGGG GCCTCATGAA 
CATTTCAATC GTTCACATGA GCGTCATCAA CATTTTACTT GTCTTGGCTC TAATGAGGAC 
TTAACCATTG ATGACATTGA ACATGTTTAT GACAATATTA GTTATGAAGA TCTGAAACAG 
ATGGGGTTCA CCAGGAGACA TGATTCTGAT GCTCCTATAA ATAAGGAAGG AGGGGATTCT 
CAAAGCCAAG AAGACATTAG TATTAGAAGA CTAAGAGAAC AGTTTGATAG AACCAGGAAC 
TCCCTAATCC TAAATAAAGA GAAAAATCTA AAGAAAGGAA TGTCCAATTC AAATTCACAA 
GAACTCAGAA TAATTGAGGA AAACATTTAT GACTCTATTC AGCTACCAGA ACAATCTCTG 
GAGCATGTAA AGCATAAATT GCATTCTAAA CGGAGCAATT TTTTGGCTCT GGGCACTGAT 
TTTCAGTCTT GTGATCAGCT TGATAGGTTT GTTTCCGAAG ACAGCCTACA GTTCAGCGAA 
GATGAATCAC TGGATCATCG TGTACCAGTG AAAGATGGAG ATTTCTGTTA TTCCTCTTCA 
AAGGCTGGTT CAGTATCACA TAAGTCTGCA GCAGATAAAT TGTCAGAGGA AGTAGATGAA 
ATCTGGAATG ATTTGGAAAA CTACATTAAG AAGAATGAGG AGAAAAAAAG TGAACGGCTT 
TTAGCATCAT TCCCTGTAAA CAAAGATGAC GTTCTTGAAA GGCGCCATGT AACGTACATG 
CCTTGTTTCA GCAAAGATAT GGAGTATTCA TTGTCGTCAT TGTGCTTGCC AGAAGATTTG 
CCTTCACAGA GGACAATACT GAATACTTCA CAAATAAGTA ATGATAATAG TAGCTGTGGA 
AGCTCAAATA GTGGCTCATT ACTAAGTCTT AAGAGGACTT CCATTGTAAG TGAAACAGGC 
TTTCTAGAAT CACCCCTTTT TGCTTCAAAC AGCACTATTC CCTATTCCGA AGATACATGT 
TCTTCTCAAA CTTTAGAGAG CACTGATAAA TCAAAAAGCA AGGTTTTTAT GATGGCTCGC 
CAATATAGTC AAAAAATAAA AAAAGCCAAC CAGCTTTTAA AGATGAAAAG CCCAGAACAA 
GAACAGCCCT TTTACAAATC ACAGAAAATG AAACCGAAGG ATCTTGCAGC TATTATGGAA 
GAGAAGAAAC AAGGAGGCTG TGCTATTGGT GCAAGAATTG CCGAGTATTC CCAGCTTTAT 
GACCAGATTA TCTTTCGCGA CTCCCCCATA AGGATGCAAA AGGAAGCAAA GAAAGACTCC 
CACAAAGTAG CATCTCCAAG TTACTCTCCA TTAAGCCCAA CTTGCCAGCA ACCGTCCTCT 
CCATCTAAAT GGCAACAGCG AGGTAAATGT CTCCAATCAA ATAGCACTGG TTTACAGTCT 
GTTAATGGAA GTGGGGCAGA ATTTTATACA TGGCCGGATG TAAAGGATTT TAAATCAAAA 
ACGGATTTAT CAGAACCTGG CTCTGGGTGC CCTCACAGAA ACATTACAGC TGCTTGTTCA 
GTGCCATCTT TGTCAAAGTC ATTACAGGAT GCATCGCATG AGTCAAGGTG GAGTGGCGTA 
AGCCAGCAGC ATAGTGAAAA TGGACAAATG GTTTGTGCTT CTAATTCACC AGAAAGGATA 
GTGAATAAAG AACCTCAGCT TTATATTCGA TCACAGTCTT CATCTAGTAT GCTACATAAC 
AGGACATCTG GCTCTCACAG ACTGGCAACA AACTCACAGC GGTCAAAGCC CATCTTAAAA 
CCTACAAGGC CTACAGATCT TGGTCATCTT GGATATGAAA GAAAATCCCA AATTGAAGAC 
GACACCTCTG ATCTTACCTT GCAAGATTCC CAGAAGGTTT TGGTCATGAA CACACTGTCC 
TCTCTTAATG CTCAAATAGC TACGCTCAAC TATTATGCTA ACTTTAAGGA TACTGATGGA 
GATGACGATG ATGATGACTA TGTAGAGATC AAGTCAGAAG ATGAAGAGGA TGAGTTAGAG 
ACTGATTCTC ATCAGGCTCT TAAAATGAGT GTAAAAGGAA AGCCAAATGT TACTCAGACT 
TACTGTAGTA CAAATCCAAC CTATTCCTTT AGCGTGCCCA GTACTCCAGT CAAGTCCTAC 
AACAGCAAGG AACTCTCTGA TGCACATATA TTAGCCTATG ACGATGCAGA CAAACTAAAT 
GACTATCTCT GGAGACCAAC GCCTTCTAAT CAGCAAAATA TTGTTCAGTC ACTTAGAGAA 
AAATTTCAGT GTCTCAGCTC AAGTAGTTTT GCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekhg1 ( XM_018094254.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017949743.1 Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G1 (plekhg1), transcript variant X3, mRNA.

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CloneID	OXa44666
Clone ID Related Accession (Same CDS sequence)	XM_018094254.1 , XM_018094254.2
Accession Version	XM_018094254.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4596bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology domain-containing family G member 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018094254.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGATA AAGCACCAGA GGGTACAGAA ACAGCATCCC AGAATGAAGA GGACTACCAT 
TTGCCTCTTT ATGGTCTTCC ATCTGTAAGA GAGCCTCAGA GTCCTAGGAC AGCAGAGGAA 
AATCTGCAAA GGACCAGCAG ACATTTCCGT TACTGTGGGA TCAAAAACAT GGAGATGTCA 
GACAGTGACC GGCCAGTCAG CTTTGGTTCA ACATCCTCAT CTGCCTCTTC CAGAGACAGT 
CACTGTTCAT TTGGCAGTCG GATGACCCTA GCATCAAACA GTCACTTGGG TTTGTTTCAC 
CAAGATAAGG AAGCTGGAGC CATAAAACTA GAACTGGTTC CTGCCCGGCA GATATCAAAT 
GATGACCTTC ATAGGATCTG CACCAAAGAA CAACCTCATG GAAGTCCAAG GGAAAAACCA 
TCTGATGAGC ATCAGAGCAC ACAGTGGAAA GCTGAAGCTA AAAGATCAAT TAAATGTTCA 
GTGACTGAAG CGGTAGAGAC AACCAGTCCT AAACTTATGT ATGTAGACAG AGTAGTTCAA 
GAAATTCTCC ATACTGAAAG GGCCTATGTG CAGGATCTGA AAAGCATTGT TGAGGATTAT 
CTCAATTGCA TTTCTGACCA ATCAAGACTT TCCCTAGGGA CAGAAGAACG GTCAGCTCTC 
TTTGGCAATA TATGCGATAT CTACCATTTC AATAGTGAAC TTCTTCAAGA ACTGGAAAAT 
TGTGATAATG ATCCTGTTGC AATAGCCGAA TGCTTTGTGT CAAAGAGTGA GGAATTCCAT 
ATATACACAC AGTATTGTAC TAATTATCCA AGATCTGTTG CTATATTGAC AGAGTGTATG 
AGAAACAAAA TACTGGCCAA ATTCTTTAGA GAACGACAGG AAGTCCTACA ACATTCTCTT 
CCCTTAGGAT CTTACCTCTT GAAGCCTGTC CAGAGAATCC TTAAGTACCA CTTGCTGTTG 
CATGAAATAT CCAATCATTT GGATAAAGAC ACTGAAGGCT ATGACATTGT GCTGGATGCC 
ATAGACACAA TGCAAAGGGT TGCATGGCAT ATAAATGATA TGAAGAGGAA ACATGAACAT 
GACATTCGGC TCCAGGAAAT CCAGAGTTTG CTCACTAATT GGAAAGGACC AGATCTGACA 
AGTTACGGGG AGCTAGTTTT GGAAGGAACA TTCCGTTTTC AGAGGGCCAA GAATGAACGC 
ACTTTGTTCC TGTTCGACCG TCTGCTTCTA ATCACAAAGA AAAGGGATGA CACATACACA 
TACAAGGCTC ATATCCTGTG TTGCAATCTC ATGCTAGTTG AAGTAATTCC AAAGGAGCCA 
CTTAGCTTCA GTGTCTTCCA TTATAAGAAC CCAAAGCTAC AGCACACAGT ACAGGCAAAG 
TCACAGCAAG ACAAGAGGCT CTGGATTTTG CATTTGAAGA GACTTATCCT TGAAAACCAC 
CCAGCGAAAA TCCCAGCAAA GGCTAAACAA GCAATTTTGG AAATGGATGC TATACAACCT 
TCGTCTAATC TACACAAAGT CATGAAGCAA GATGGTTTAA GCCCAGAGAT GCAAAAGAGA 
AGTAGCATAC AGGGCAATTT GCTATGCCAG GCTTCTAAAC TCAGTGGAAG TGATCTGCTT 
CGTGGGTCTG GGATGAGAAA AGGTGGCTCA ATGAATCACA ACAGCAATAT GACCCGGTCT 
CAAGATTTCT ACAACAAAGA AGGTCTAAGT GTGCGCCTTG GGCCAGCGGA GCAGTCTGAC 
GCAGATGATG AAGAGGAGTC TGAACAGCTG AGCTCTCAGC ATAAAATTAA AGGTGGCAGA 
AAAAGACTTA ACAGCCAAGC TTCAGAGAAT GTTGAGAAGC GAAGAAGTAT CAATCTAAGT 
GTATGTGAGA GCCAGATATC AAAAAGCCAG TCAGATGAAG AGTCAACAAA GCTGAATACA 
GGGCAGATCT TTCCAAGTTC CAGTGGTCAT CTTCCAAGCA GACAGAATGG TGCACAACAG 
AGCAATTCTT TGATTATGAA TATCCTTGGA GGTACTGGTG CTCTGAGGAA CATCTGGACT 
GATCATCAAA TTAGACAAGC CCTCTTTCCT TCTAGACGCT CTACACAGGA AAATGAGGAG 
GATGATGATG ACTACCAGAT GTTTCTGTTG TCTGAATCTG CAACCAGGTT GGAATCGTCA 
TGGTCACAGG AAGAAAGAGT GGCATCAAGC CGGCCATGTA GCTGGCACCT TGGTCTTATG 
GAAGAAGACA CAATACCAAA CCCCTCCTGC AAAATTGTCA GGCGTGCAAG TAGTGTTGGA 
GAGAATAAAT CTTGTTCTGC AAGACAACGT AGCCACTATC ATAAATCGGG GCCTCATGAA 
CATTTCAATC GTTCACATGA GCGTCATCAA CATTTTACTT GTCTTGGCTC TAATGAGGAC 
TTAACCATTG ATGACATTGA ACATGTTTAT GACAATATTA GTTATGAAGA TCTGAAACAG 
ATGGGGTTCA CCAGGAGACA TGATTCTGAT GCTCCTATAA ATAAGGAAGG AGGGGATTCT 
CAAAGCCAAG AAGACATTAG TATTAGAAGA CTAAGAGAAC AGTTTGATAG AACCAGGAAC 
TCCCTAATCC TAAATAAAGA GAAAAATCTA AAGAAAGGAA TGTCCAATTC AAATTCACAA 
GAACTCAGAA TAATTGAGGA AAACATTTAT GACTCTATTC AGCTACCAGA ACAATCTCTG 
GAGCATGTAA AGCATAAATT GCATTCTAAA CGGAGCAATT TTTTGGCTCT GGGCACTGAT 
TTTCAGTCTT GTGATCAGCT TGATAGGTTT GTTTCCGAAG ACAGCCTACA GTTCAGCGAA 
GATGAATCAC TGGATCATCG TGTACCAGTG AAAGATGGAG ATTTCTGTTA TTCCTCTTCA 
AAGGCTGGTT CAGTATCACA TAAGTCTGCA GCAGATAAAT TGTCAGAGGA AGTAGATGAA 
ATCTGGAATG ATTTGGAAAA CTACATTAAG AAGAATGAGG AGAAAAAAAG TGAACGGCTT 
TTAGCATCAT TCCCTGTAAA CAAAGATGAC GTTCTTGAAA GGCGCCATGT AACGTACATG 
CCTTGTTTCA GCAAAGATAT GGAGTATTCA TTGTCGTCAT TGTGCTTGCC AGAAGATTTG 
CCTTCACAGA GGACAATACT GAATACTTCA CAAATAAGTA ATGATAATAG TAGCTGTGGA 
AGCTCAAATA GTGGCTCATT ACTAAGTCTT AAGAGGACTT CCATTGTAAG TGAAACAGGC 
TTTCTAGAAT CACCCCTTTT TGCTTCAAAC AGCACTATTC CCTATTCCGA AGATACATGT 
TCTTCTCAAA CTTTAGAGAG CACTGATAAA TCAAAAAGCA AGGTTTTTAT GATGGCTCGC 
CAATATAGTC AAAAAATAAA AAAAGCCAAC CAGCTTTTAA AGATGAAAAG CCCAGAACAA 
GAACAGCCCT TTTACAAATC ACAGAAAATG AAACCGAAGG ATCTTGCAGC TATTATGGAA 
GAGAAGAAAC AAGGAGGCTG TGCTATTGGT GCAAGAATTG CCGAGTATTC CCAGCTTTAT 
GACCAGATTA TCTTTCGCGA CTCCCCCATA AGGATGCAAA AGGAAGCAAA GAAAGACTCC 
CACAAAGTAG CATCTCCAAG TTACTCTCCA TTAAGCCCAA CTTGCCAGCA ACCGTCCTCT 
CCATCTAAAT GGCAACAGCG AGGTAAATGT CTCCAATCAA ATAGCACTGG TTTACAGTCT 
GTTAATGGAA GTGGGGCAGA ATTTTATACA TGGCCGGATG TAAAGGATTT TAAATCAAAA 
ACGGATTTAT CAGAACCTGG CTCTGGGTGC CCTCACAGAA ACATTACAGC TGCTTGTTCA 
GTGCCATCTT TGTCAAAGTC ATTACAGGAT GCATCGCATG AGTCAAGGTG GAGTGGCGTA 
AGCCAGCAGC ATAGTGAAAA TGGACAAATG GTTTGTGCTT CTAATTCACC AGAAAGGATA 
GTGAATAAAG AACCTCAGCT TTATATTCGA TCACAGTCTT CATCTAGTAT GCTACATAAC 
AGGACATCTG GCTCTCACAG ACTGGCAACA AACTCACAGC GGTCAAAGCC CATCTTAAAA 
CCTACAAGGC CTACAGATCT TGGTCATCTT GGATATGAAA GAAAATCCCA AATTGAAGAC 
GACACCTCTG ATCTTACCTT GCAAGATTCC CAGAAGGTTT TGGTCATGAA CACACTGTCC 
TCTCTTAATG CTCAAATAGC TACGCTCAAC TATTATGCTA ACTTTAAGGA TACTGATGGA 
GATGACGATG ATGATGACTA TGTAGAGATC AAGTCAGAAG ATGAAGAGGA TGAGTTAGAG 
ACTGATTCTC ATCAGGCTCT TAAAATGAGT GTAAAAGGAA AGCCAAATGT TACTCAGACT 
TACTGTAGTA CAAATCCAAC CTATTCCTTT AGCGTGCCCA GTACTCCAGT CAAGTCCTAC 
AACAGCAAGG AACTCTCTGA TGCACATATA TTAGCCTATG ACGATGCAGA CAAACTAAAT 
GACTATCTCT GGAGACCAAC GCCTTCTAAT CAGCAAAATA TTGTTCAGTC ACTTAGAGAA 
AAATTTCAGT GTCTCAGCTC AAGTAGTTTT GCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017949743.1
CDS	258..4853
Translation	MPDKAPEGTETASQNEEDYHLPLYGLPSVREPQSPRTAEENLQRTSRHFRYCGIKNMEMSDSDRPVSFGSTSSSASSRDSHCSFGSRMTLASNSHLGLFHQDKEAGAIKLELVPARQISNDDLHRICTKEQPHGSPREKPSDEHQSTQWKAEAKRSIKCSVTEAVETTSPKLMYVDRVVQEILHTERAYVQDLKSIVEDYLNCISDQSRLSLGTEERSALFGNICDIYHFNSELLQELENCDNDPVAIAECFVSKSEEFHIYTQYCTNYPRSVAILTECMRNKILAKFFRERQEVLQHSLPLGSYLLKPVQRILKYHLLLHEISNHLDKDTEGYDIVLDAIDTMQRVAWHINDMKRKHEHDIRLQEIQSLLTNWKGPDLTSYGELVLEGTFRFQRAKNERTLFLFDRLLLITKKRDDTYTYKAHILCCNLMLVEVIPKEPLSFSVFHYKNPKLQHTVQAKSQQDKRLWILHLKRLILENHPAKIPAKAKQAILEMDAIQPSSNLHKVMKQDGLSPEMQKRSSIQGNLLCQASKLSGSDLLRGSGMRKGGSMNHNSNMTRSQDFYNKEGLSVRLGPAEQSDADDEEESEQLSSQHKIKGGRKRLNSQASENVEKRRSINLSVCESQISKSQSDEESTKLNTGQIFPSSSGHLPSRQNGAQQSNSLIMNILGGTGALRNIWTDHQIRQALFPSRRSTQENEEDDDDYQMFLLSESATRLESSWSQEERVASSRPCSWHLGLMEEDTIPNPSCKIVRRASSVGENKSCSARQRSHYHKSGPHEHFNRSHERHQHFTCLGSNEDLTIDDIEHVYDNISYEDLKQMGFTRRHDSDAPINKEGGDSQSQEDISIRRLREQFDRTRNSLILNKEKNLKKGMSNSNSQELRIIEENIYDSIQLPEQSLEHVKHKLHSKRSNFLALGTDFQSCDQLDRFVSEDSLQFSEDESLDHRVPVKDGDFCYSSSKAGSVSHKSAADKLSEEVDEIWNDLENYIKKNEEKKSERLLASFPVNKDDVLERRHVTYMPCFSKDMEYSLSSLCLPEDLPSQRTILNTSQISNDNSSCGSSNSGSLLSLKRTSIVSETGFLESPLFASNSTIPYSEDTCSSQTLESTDKSKSKVFMMARQYSQKIKKANQLLKMKSPEQEQPFYKSQKMKPKDLAAIMEEKKQGGCAIGARIAEYSQLYDQIIFRDSPIRMQKEAKKDSHKVASPSYSPLSPTCQQPSSPSKWQQRGKCLQSNSTGLQSVNGSGAEFYTWPDVKDFKSKTDLSEPGSGCPHRNITAACSVPSLSKSLQDASHESRWSGVSQQHSENGQMVCASNSPERIVNKEPQLYIRSQSSSSMLHNRTSGSHRLATNSQRSKPILKPTRPTDLGHLGYERKSQIEDDTSDLTLQDSQKVLVMNTLSSLNAQIATLNYYANFKDTDGDDDDDDYVEIKSEDEEDELETDSHQALKMSVKGKPNVTQTYCSTNPTYSFSVPSTPVKSYNSKELSDAHILAYDDADKLNDYLWRPTPSNQQNIVQSLREKFQCLSSSSFA

Target ORF information:

RefSeq Version	XM_018094254.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G1 (plekhg1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018094254.2

ORF Insert Sequence:

ATGCCAGATA AAGCACCAGA GGGTACAGAA ACAGCATCCC AGAATGAAGA GGACTACCAT 
TTGCCTCTTT ATGGTCTTCC ATCTGTAAGA GAGCCTCAGA GTCCTAGGAC AGCAGAGGAA 
AATCTGCAAA GGACCAGCAG ACATTTCCGT TACTGTGGGA TCAAAAACAT GGAGATGTCA 
GACAGTGACC GGCCAGTCAG CTTTGGTTCA ACATCCTCAT CTGCCTCTTC CAGAGACAGT 
CACTGTTCAT TTGGCAGTCG GATGACCCTA GCATCAAACA GTCACTTGGG TTTGTTTCAC 
CAAGATAAGG AAGCTGGAGC CATAAAACTA GAACTGGTTC CTGCCCGGCA GATATCAAAT 
GATGACCTTC ATAGGATCTG CACCAAAGAA CAACCTCATG GAAGTCCAAG GGAAAAACCA 
TCTGATGAGC ATCAGAGCAC ACAGTGGAAA GCTGAAGCTA AAAGATCAAT TAAATGTTCA 
GTGACTGAAG CGGTAGAGAC AACCAGTCCT AAACTTATGT ATGTAGACAG AGTAGTTCAA 
GAAATTCTCC ATACTGAAAG GGCCTATGTG CAGGATCTGA AAAGCATTGT TGAGGATTAT 
CTCAATTGCA TTTCTGACCA ATCAAGACTT TCCCTAGGGA CAGAAGAACG GTCAGCTCTC 
TTTGGCAATA TATGCGATAT CTACCATTTC AATAGTGAAC TTCTTCAAGA ACTGGAAAAT 
TGTGATAATG ATCCTGTTGC AATAGCCGAA TGCTTTGTGT CAAAGAGTGA GGAATTCCAT 
ATATACACAC AGTATTGTAC TAATTATCCA AGATCTGTTG CTATATTGAC AGAGTGTATG 
AGAAACAAAA TACTGGCCAA ATTCTTTAGA GAACGACAGG AAGTCCTACA ACATTCTCTT 
CCCTTAGGAT CTTACCTCTT GAAGCCTGTC CAGAGAATCC TTAAGTACCA CTTGCTGTTG 
CATGAAATAT CCAATCATTT GGATAAAGAC ACTGAAGGCT ATGACATTGT GCTGGATGCC 
ATAGACACAA TGCAAAGGGT TGCATGGCAT ATAAATGATA TGAAGAGGAA ACATGAACAT 
GACATTCGGC TCCAGGAAAT CCAGAGTTTG CTCACTAATT GGAAAGGACC AGATCTGACA 
AGTTACGGGG AGCTAGTTTT GGAAGGAACA TTCCGTTTTC AGAGGGCCAA GAATGAACGC 
ACTTTGTTCC TGTTCGACCG TCTGCTTCTA ATCACAAAGA AAAGGGATGA CACATACACA 
TACAAGGCTC ATATCCTGTG TTGCAATCTC ATGCTAGTTG AAGTAATTCC AAAGGAGCCA 
CTTAGCTTCA GTGTCTTCCA TTATAAGAAC CCAAAGCTAC AGCACACAGT ACAGGCAAAG 
TCACAGCAAG ACAAGAGGCT CTGGATTTTG CATTTGAAGA GACTTATCCT TGAAAACCAC 
CCAGCGAAAA TCCCAGCAAA GGCTAAACAA GCAATTTTGG AAATGGATGC TATACAACCT 
TCGTCTAATC TACACAAAGT CATGAAGCAA GATGGTTTAA GCCCAGAGAT GCAAAAGAGA 
AGTAGCATAC AGGGCAATTT GCTATGCCAG GCTTCTAAAC TCAGTGGAAG TGATCTGCTT 
CGTGGGTCTG GGATGAGAAA AGGTGGCTCA ATGAATCACA ACAGCAATAT GACCCGGTCT 
CAAGATTTCT ACAACAAAGA AGGTCTAAGT GTGCGCCTTG GGCCAGCGGA GCAGTCTGAC 
GCAGATGATG AAGAGGAGTC TGAACAGCTG AGCTCTCAGC ATAAAATTAA AGGTGGCAGA 
AAAAGACTTA ACAGCCAAGC TTCAGAGAAT GTTGAGAAGC GAAGAAGTAT CAATCTAAGT 
GTATGTGAGA GCCAGATATC AAAAAGCCAG TCAGATGAAG AGTCAACAAA GCTGAATACA 
GGGCAGATCT TTCCAAGTTC CAGTGGTCAT CTTCCAAGCA GACAGAATGG TGCACAACAG 
AGCAATTCTT TGATTATGAA TATCCTTGGA GGTACTGGTG CTCTGAGGAA CATCTGGACT 
GATCATCAAA TTAGACAAGC CCTCTTTCCT TCTAGACGCT CTACACAGGA AAATGAGGAG 
GATGATGATG ACTACCAGAT GTTTCTGTTG TCTGAATCTG CAACCAGGTT GGAATCGTCA 
TGGTCACAGG AAGAAAGAGT GGCATCAAGC CGGCCATGTA GCTGGCACCT TGGTCTTATG 
GAAGAAGACA CAATACCAAA CCCCTCCTGC AAAATTGTCA GGCGTGCAAG TAGTGTTGGA 
GAGAATAAAT CTTGTTCTGC AAGACAACGT AGCCACTATC ATAAATCGGG GCCTCATGAA 
CATTTCAATC GTTCACATGA GCGTCATCAA CATTTTACTT GTCTTGGCTC TAATGAGGAC 
TTAACCATTG ATGACATTGA ACATGTTTAT GACAATATTA GTTATGAAGA TCTGAAACAG 
ATGGGGTTCA CCAGGAGACA TGATTCTGAT GCTCCTATAA ATAAGGAAGG AGGGGATTCT 
CAAAGCCAAG AAGACATTAG TATTAGAAGA CTAAGAGAAC AGTTTGATAG AACCAGGAAC 
TCCCTAATCC TAAATAAAGA GAAAAATCTA AAGAAAGGAA TGTCCAATTC AAATTCACAA 
GAACTCAGAA TAATTGAGGA AAACATTTAT GACTCTATTC AGCTACCAGA ACAATCTCTG 
GAGCATGTAA AGCATAAATT GCATTCTAAA CGGAGCAATT TTTTGGCTCT GGGCACTGAT 
TTTCAGTCTT GTGATCAGCT TGATAGGTTT GTTTCCGAAG ACAGCCTACA GTTCAGCGAA 
GATGAATCAC TGGATCATCG TGTACCAGTG AAAGATGGAG ATTTCTGTTA TTCCTCTTCA 
AAGGCTGGTT CAGTATCACA TAAGTCTGCA GCAGATAAAT TGTCAGAGGA AGTAGATGAA 
ATCTGGAATG ATTTGGAAAA CTACATTAAG AAGAATGAGG AGAAAAAAAG TGAACGGCTT 
TTAGCATCAT TCCCTGTAAA CAAAGATGAC GTTCTTGAAA GGCGCCATGT AACGTACATG 
CCTTGTTTCA GCAAAGATAT GGAGTATTCA TTGTCGTCAT TGTGCTTGCC AGAAGATTTG 
CCTTCACAGA GGACAATACT GAATACTTCA CAAATAAGTA ATGATAATAG TAGCTGTGGA 
AGCTCAAATA GTGGCTCATT ACTAAGTCTT AAGAGGACTT CCATTGTAAG TGAAACAGGC 
TTTCTAGAAT CACCCCTTTT TGCTTCAAAC AGCACTATTC CCTATTCCGA AGATACATGT 
TCTTCTCAAA CTTTAGAGAG CACTGATAAA TCAAAAAGCA AGGTTTTTAT GATGGCTCGC 
CAATATAGTC AAAAAATAAA AAAAGCCAAC CAGCTTTTAA AGATGAAAAG CCCAGAACAA 
GAACAGCCCT TTTACAAATC ACAGAAAATG AAACCGAAGG ATCTTGCAGC TATTATGGAA 
GAGAAGAAAC AAGGAGGCTG TGCTATTGGT GCAAGAATTG CCGAGTATTC CCAGCTTTAT 
GACCAGATTA TCTTTCGCGA CTCCCCCATA AGGATGCAAA AGGAAGCAAA GAAAGACTCC 
CACAAAGTAG CATCTCCAAG TTACTCTCCA TTAAGCCCAA CTTGCCAGCA ACCGTCCTCT 
CCATCTAAAT GGCAACAGCG AGGTAAATGT CTCCAATCAA ATAGCACTGG TTTACAGTCT 
GTTAATGGAA GTGGGGCAGA ATTTTATACA TGGCCGGATG TAAAGGATTT TAAATCAAAA 
ACGGATTTAT CAGAACCTGG CTCTGGGTGC CCTCACAGAA ACATTACAGC TGCTTGTTCA 
GTGCCATCTT TGTCAAAGTC ATTACAGGAT GCATCGCATG AGTCAAGGTG GAGTGGCGTA 
AGCCAGCAGC ATAGTGAAAA TGGACAAATG GTTTGTGCTT CTAATTCACC AGAAAGGATA 
GTGAATAAAG AACCTCAGCT TTATATTCGA TCACAGTCTT CATCTAGTAT GCTACATAAC 
AGGACATCTG GCTCTCACAG ACTGGCAACA AACTCACAGC GGTCAAAGCC CATCTTAAAA 
CCTACAAGGC CTACAGATCT TGGTCATCTT GGATATGAAA GAAAATCCCA AATTGAAGAC 
GACACCTCTG ATCTTACCTT GCAAGATTCC CAGAAGGTTT TGGTCATGAA CACACTGTCC 
TCTCTTAATG CTCAAATAGC TACGCTCAAC TATTATGCTA ACTTTAAGGA TACTGATGGA 
GATGACGATG ATGATGACTA TGTAGAGATC AAGTCAGAAG ATGAAGAGGA TGAGTTAGAG 
ACTGATTCTC ATCAGGCTCT TAAAATGAGT GTAAAAGGAA AGCCAAATGT TACTCAGACT 
TACTGTAGTA CAAATCCAAC CTATTCCTTT AGCGTGCCCA GTACTCCAGT CAAGTCCTAC 
AACAGCAAGG AACTCTCTGA TGCACATATA TTAGCCTATG ACGATGCAGA CAAACTAAAT 
GACTATCTCT GGAGACCAAC GCCTTCTAAT CAGCAAAATA TTGTTCAGTC ACTTAGAGAA 
AAATTTCAGT GTCTCAGCTC AAGTAGTTTT GCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekhg1 ( XM_031901989.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757849.1 Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G1 (plekhg1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa58264
Clone ID Related Accession (Same CDS sequence)	XM_031901989.1
Accession Version	XM_031901989.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology domain-containing family G member 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGATGT CAGACAGTGA CCGGCCAGTC AGCTTTGGTT CAACATCCTC ATCTGCCTCT 
TCCAGAGACA GTCACTGTTC ATTTGGCAGT CGGATGACCC TAGCATCAAA CAGTCACTTG 
GGTTTGTTTC ACCAAGATAA GGAAGCTGGA GCCATAAAAC TAGAACTGGT TCCTGCCCGG 
CAGATATCAA ATGATGACCT TCATAGGATC TGCACCAAAG AACAACCTCA TGGAAGTCCA 
AGGGAAAAAC CATCTGATGA GCATCAGAGC ACACAGTGGA AAGCTGAAGC TAAAAGATCA 
ATTAAATGTT CAGTGACTGA AGCGGTAGAG ACAACCAGTC CTAAACTTAT GTATGTAGAC 
AGAGTAGTTC AAGAAATTCT CCATACTGAA AGGGCCTATG TGCAGGATCT GAAAAGCATT 
GTTGAGGATT ATCTCAATTG CATTTCTGAC CAATCAAGAC TTTCCCTAGG GACAGAAGAA 
CGGTCAGCTC TCTTTGGCAA TATATGCGAT ATCTACCATT TCAATAGTGA ACTTCTTCAA 
GAACTGGAAA ATTGTGATAA TGATCCTGTT GCAATAGCCG AATGCTTTGT GTCAAAGAGT 
GAGGAATTCC ATATATACAC ACAGTATTGT ACTAATTATC CAAGATCTGT TGCTATATTG 
ACAGAGTGTA TGAGAAACAA AATACTGGCC AAATTCTTTA GAGAACGACA GGAAGTCCTA 
CAACATTCTC TTCCCTTAGG ATCTTACCTC TTGAAGCCTG TCCAGAGAAT CCTTAAGTAC 
CACTTGCTGT TGCATGAAAT ATCCAATCAT TTGGATAAAG ACACTGAAGG CTATGACATT 
GTGCTGGATG CCATAGACAC AATGCAAAGG GTTGCATGGC ATATAAATGA TATGAAGAGG 
AAACATGAAC ATGACATTCG GCTCCAGGAA ATCCAGAGTT TGCTCACTAA TTGGAAAGGA 
CCAGATCTGA CAAGTTACGG GGAGCTAGTT TTGGAAGGAA CATTCCGTTT TCAGAGGGCC 
AAGAATGAAC GCACTTTGTT CCTGTTCGAC CGTCTGCTTC TAATCACAAA GAAAAGGGAT 
GACACATACA CATACAAGGC TCATATCCTG TGTTGCAATC TCATGCTAGT TGAAGTAATT 
CCAAAGGAGC CACTTAGCTT CAGTGTCTTC CATTATAAGA ACCCAAAGCT ACAGCACACA 
GTACAGGCAA AGTCACAGCA AGACAAGAGG CTCTGGATTT TGCATTTGAA GAGACTTATC 
CTTGAAAACC ACCCAGCGAA AATCCCAGCA AAGGCTAAAC AAGCAATTTT GGAAATGGAT 
GCTATACATC ATCCCGGGTT CTCCTATACC CCAGAGGGAG AACGGAAATC ACCTTTAAAC 
TTGAAAGAAG ATCTCGCATC ATGCAGAGTC AGAAGAAAGT CAGAACCTTC GTCTAATCTA 
CACAAAGTCA TGAAGCAAGA TGGTTTAAGC CCAGAGATGC AAAAGAGAAG TAGCATACAG 
GGCAATTTGC TATGCCAGGC TTCTAAACTC AGTGGAAGTG ATCTGCTTCG TGGGTCTGGG 
ATGAGAAAAG GTGGCTCAAT GAATCACAAC AGCAATATGA CCCGGTCTCA AGATTTCTAC 
AACAAAGAAG GTCTAAGTGT GCGCCTTGGG CCAGCGGAGC AGTCTGACGC AGATGATGAA 
GAGGAGTCTG AACAGCTGAG CTCTCAGCAT AAAATTAAAG GTGGCAGAAA AAGACTTAAC 
AGCCAAGCTT CAGAGAATGT TGAGAAGCGA AGAAGTATCA ATCTAAGTGT ATGTGAGAGC 
CAGATATCAA AAAGCCAGTC AGATGAAGAG TCAACAAAGC TGAATACAGG GCAGATCTTT 
CCAAGTTCCA GTGGTCATCT TCCAAGCAGA CAGAATGGTG CACAACAGAG CAATTCTTTG 
ATTATGAATA TCCTTGGAGG TACTGGTGCT CTGAGGAACA TCTGGACTGA TCATCAAATT 
AGACAAGCCC TCTTTCCTTC TAGACGCTCT ACACAGGAAA ATGAGGAGGA TGATGATGAC 
TACCAGATGT TTCTGTTGTC TGAATCTGCA ACCAGGTTGG AATCGTCATG GTCACAGGAA 
GAAAGAGTGG CATCAAGCCG GCCATGTAGC TGGCACCTTG GTCTTATGGA AGAAGACACA 
ATACCAAACC CCTCCTGCAA AATTGTCAGG CGTGCAAGTA GTGTTGGAGA GAATAAATCT 
TGTTCTGCAA GACAACGTAG CCACTATCAT AAATCGGGGC CTCATGAACA TTTCAATCGT 
TCACATGAGC GTCATCAACA TTTTACTTGT CTTGGCTCTA ATGAGGACTT AACCATTGAT 
GACATTGAAC ATGTTTATGA CAATATTAGT TATGAAGATC TGAAACAGAT GGGGTTCACC 
AGGAGACATG ATTCTGATGC TCCTATAAAT AAGGAAGGAG GGGATTCTCA AAGCCAAGAA 
GACATTAGTA TTAGAAGACT AAGAGAACAG TTTGATAGAA CCAGGAACTC CCTAATCCTA 
AATAAAGAGA AAAATCTAAA GAAAGGAATG TCCAATTCAA ATTCACAAGA ACTCAGAATA 
ATTGAGGAAA ACATTTATGA CTCTATTCAG CTACCAGAAC AATCTCTGGA GCATGTAAAG 
CATAAATTGC ATTCTAAACG GAGCAATTTT TTGGCTCTGG GCACTGATTT TCAGTCTTGT 
GATCAGCTTG ATAGGTTTGT TTCCGAAGAC AGCCTACAGT TCAGCGAAGA TGAATCACTG 
GATCATCGTG TACCAGTGAA AGATGGAGAT TTCTGTTATT CCTCTTCAAA GGCTGGTTCA 
GTATCACATA AGTCTGCAGC AGATAAATTG TCAGAGGAAG TAGATGAAAT CTGGAATGAT 
TTGGAAAACT ACATTAAGAA GAATGAGGAG AAAAAAAGTG AACGGCTTTT AGCATCATTC 
CCTGTAAACA AAGATGACGT TCTTGAAAGG CGCCATGTAA CGTACATGCC TTGTTTCAGC 
AAAGATATGG AGTATTCATT GTCGTCATTG TGCTTGCCAG AAGATTTGCC TTCACAGAGG 
ACAATACTGA ATACTTCACA AATAAGTAAT GATAATAGTA GCTGTGGAAG CTCAAATAGT 
GGCTCATTAC TAAGTCTTAA GAGGACTTCC ATTGTAAGTG AAACAGGCTT TCTAGAATCA 
CCCCTTTTTG CTTCAAACAG CACTATTCCC TATTCCGAAG ATACATGTTC TTCTCAAACT 
TTAGAGAGCA CTGATAAATC AAAAAGCAAG GTTTTTATGA TGGCTCGCCA ATATAGTCAA 
AAAATAAAAA AAGCCAACCA GCTTTTAAAG ATGAAAAGCC CAGAACAAGA ACAGCCCTTT 
TACAAATCAC AGAAAATGAA ACCGAAGGAT CTTGCAGCTA TTATGGAAGA GAAGAAACAA 
GGAGGCTGTG CTATTGGTGC AAGAATTGCC GAGTATTCCC AGCTTTATGA CCAGATTATC 
TTTCGCGACT CCCCCATAAG GATGCAAAAG GAAGCAAAGA AAGACTCCCA CAAAGTAGCA 
TCTCCAAGTT ACTCTCCATT AAGCCCAACT TGCCAGCAAC CGTCCTCTCC ATCTAAATGG 
CAACAGCGAG GTAAATGTCT CCAATCAAAT AGCACTGGTT TACAGTCTGT TAATGGAAGT 
GGGGCAGAAT TTTATACATG GCCGGATGTA AAGGATTTTA AATCAAAAAC GGATTTATCA 
GAACCTGGCT CTGGGTGCCC TCACAGAAAC ATTACAGCTG CTTGTTCAGT GCCATCTTTG 
TCAAAGTCAT TACAGGATGC ATCGCATGAG TCAAGGTGGA GTGGCGTAAG CCAGCAGCAT 
AGTGAAAATG GACAAATGGT TTGTGCTTCT AATTCACCAG AAAGGATAGT GAATAAAGAA 
CCTCAGCTTT ATATTCGATC ACAGTCTTCA TCTAGTATGC TACATAACAG GACATCTGGC 
TCTCACAGAC TGGCAACAAA CTCACAGCGG TCAAAGCCCA TCTTAAAACC TACAAGGCCT 
ACAGATCTTG GTCATCTTGG ATATGAAAGA AAATCCCAAA TTGAAGACGA CACCTCTGAT 
CTTACCTTGC AAGATTCCCA GAAGGTTTTG GTCATGAACA CACTGTCCTC TCTTAATGCT 
CAAATAGCTA CGCTCAACTA TTATGCTAAC TTTAAGGATA CTGATGGAGA TGACGATGAT 
GATGACTATG TAGAGATCAA GTCAGAAGAT GAAGAGGATG AGTTAGAGAC TGATTCTCAT 
CAGGCTCTTA AAATGAGTGT AAAAGGAAAG CCAAATGTTA CTCAGACTTA CTGTAGTACA 
AATCCAACCT ATTCCTTTAG CGTGCCCAGT ACTCCAGTCA AGTCCTACAA CAGCAAGGAA 
CTCTCTGATG CACATATATT AGCCTATGAC GATGCAGACA AACTAAATGA CTATCTCTGG 
AGACCAACGC CTTCTAATCA GCAAAATATT GTTCAGTCAC TTAGAGAAAA ATTTCAGTGT 
CTCAGCTCAA GTAGTTTTGC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757849.1
CDS	236..4759
Translation	MEMSDSDRPVSFGSTSSSASSRDSHCSFGSRMTLASNSHLGLFHQDKEAGAIKLELVPARQISNDDLHRICTKEQPHGSPREKPSDEHQSTQWKAEAKRSIKCSVTEAVETTSPKLMYVDRVVQEILHTERAYVQDLKSIVEDYLNCISDQSRLSLGTEERSALFGNICDIYHFNSELLQELENCDNDPVAIAECFVSKSEEFHIYTQYCTNYPRSVAILTECMRNKILAKFFRERQEVLQHSLPLGSYLLKPVQRILKYHLLLHEISNHLDKDTEGYDIVLDAIDTMQRVAWHINDMKRKHEHDIRLQEIQSLLTNWKGPDLTSYGELVLEGTFRFQRAKNERTLFLFDRLLLITKKRDDTYTYKAHILCCNLMLVEVIPKEPLSFSVFHYKNPKLQHTVQAKSQQDKRLWILHLKRLILENHPAKIPAKAKQAILEMDAIHHPGFSYTPEGERKSPLNLKEDLASCRVRRKSEPSSNLHKVMKQDGLSPEMQKRSSIQGNLLCQASKLSGSDLLRGSGMRKGGSMNHNSNMTRSQDFYNKEGLSVRLGPAEQSDADDEEESEQLSSQHKIKGGRKRLNSQASENVEKRRSINLSVCESQISKSQSDEESTKLNTGQIFPSSSGHLPSRQNGAQQSNSLIMNILGGTGALRNIWTDHQIRQALFPSRRSTQENEEDDDDYQMFLLSESATRLESSWSQEERVASSRPCSWHLGLMEEDTIPNPSCKIVRRASSVGENKSCSARQRSHYHKSGPHEHFNRSHERHQHFTCLGSNEDLTIDDIEHVYDNISYEDLKQMGFTRRHDSDAPINKEGGDSQSQEDISIRRLREQFDRTRNSLILNKEKNLKKGMSNSNSQELRIIEENIYDSIQLPEQSLEHVKHKLHSKRSNFLALGTDFQSCDQLDRFVSEDSLQFSEDESLDHRVPVKDGDFCYSSSKAGSVSHKSAADKLSEEVDEIWNDLENYIKKNEEKKSERLLASFPVNKDDVLERRHVTYMPCFSKDMEYSLSSLCLPEDLPSQRTILNTSQISNDNSSCGSSNSGSLLSLKRTSIVSETGFLESPLFASNSTIPYSEDTCSSQTLESTDKSKSKVFMMARQYSQKIKKANQLLKMKSPEQEQPFYKSQKMKPKDLAAIMEEKKQGGCAIGARIAEYSQLYDQIIFRDSPIRMQKEAKKDSHKVASPSYSPLSPTCQQPSSPSKWQQRGKCLQSNSTGLQSVNGSGAEFYTWPDVKDFKSKTDLSEPGSGCPHRNITAACSVPSLSKSLQDASHESRWSGVSQQHSENGQMVCASNSPERIVNKEPQLYIRSQSSSSMLHNRTSGSHRLATNSQRSKPILKPTRPTDLGHLGYERKSQIEDDTSDLTLQDSQKVLVMNTLSSLNAQIATLNYYANFKDTDGDDDDDDYVEIKSEDEEDELETDSHQALKMSVKGKPNVTQTYCSTNPTYSFSVPSTPVKSYNSKELSDAHILAYDDADKLNDYLWRPTPSNQQNIVQSLREKFQCLSSSSFA

Target ORF information:

RefSeq Version	XM_031901989.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G1 (plekhg1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031901989.1

ORF Insert Sequence:

ATGGAGATGT CAGACAGTGA CCGGCCAGTC AGCTTTGGTT CAACATCCTC ATCTGCCTCT 
TCCAGAGACA GTCACTGTTC ATTTGGCAGT CGGATGACCC TAGCATCAAA CAGTCACTTG 
GGTTTGTTTC ACCAAGATAA GGAAGCTGGA GCCATAAAAC TAGAACTGGT TCCTGCCCGG 
CAGATATCAA ATGATGACCT TCATAGGATC TGCACCAAAG AACAACCTCA TGGAAGTCCA 
AGGGAAAAAC CATCTGATGA GCATCAGAGC ACACAGTGGA AAGCTGAAGC TAAAAGATCA 
ATTAAATGTT CAGTGACTGA AGCGGTAGAG ACAACCAGTC CTAAACTTAT GTATGTAGAC 
AGAGTAGTTC AAGAAATTCT CCATACTGAA AGGGCCTATG TGCAGGATCT GAAAAGCATT 
GTTGAGGATT ATCTCAATTG CATTTCTGAC CAATCAAGAC TTTCCCTAGG GACAGAAGAA 
CGGTCAGCTC TCTTTGGCAA TATATGCGAT ATCTACCATT TCAATAGTGA ACTTCTTCAA 
GAACTGGAAA ATTGTGATAA TGATCCTGTT GCAATAGCCG AATGCTTTGT GTCAAAGAGT 
GAGGAATTCC ATATATACAC ACAGTATTGT ACTAATTATC CAAGATCTGT TGCTATATTG 
ACAGAGTGTA TGAGAAACAA AATACTGGCC AAATTCTTTA GAGAACGACA GGAAGTCCTA 
CAACATTCTC TTCCCTTAGG ATCTTACCTC TTGAAGCCTG TCCAGAGAAT CCTTAAGTAC 
CACTTGCTGT TGCATGAAAT ATCCAATCAT TTGGATAAAG ACACTGAAGG CTATGACATT 
GTGCTGGATG CCATAGACAC AATGCAAAGG GTTGCATGGC ATATAAATGA TATGAAGAGG 
AAACATGAAC ATGACATTCG GCTCCAGGAA ATCCAGAGTT TGCTCACTAA TTGGAAAGGA 
CCAGATCTGA CAAGTTACGG GGAGCTAGTT TTGGAAGGAA CATTCCGTTT TCAGAGGGCC 
AAGAATGAAC GCACTTTGTT CCTGTTCGAC CGTCTGCTTC TAATCACAAA GAAAAGGGAT 
GACACATACA CATACAAGGC TCATATCCTG TGTTGCAATC TCATGCTAGT TGAAGTAATT 
CCAAAGGAGC CACTTAGCTT CAGTGTCTTC CATTATAAGA ACCCAAAGCT ACAGCACACA 
GTACAGGCAA AGTCACAGCA AGACAAGAGG CTCTGGATTT TGCATTTGAA GAGACTTATC 
CTTGAAAACC ACCCAGCGAA AATCCCAGCA AAGGCTAAAC AAGCAATTTT GGAAATGGAT 
GCTATACATC ATCCCGGGTT CTCCTATACC CCAGAGGGAG AACGGAAATC ACCTTTAAAC 
TTGAAAGAAG ATCTCGCATC ATGCAGAGTC AGAAGAAAGT CAGAACCTTC GTCTAATCTA 
CACAAAGTCA TGAAGCAAGA TGGTTTAAGC CCAGAGATGC AAAAGAGAAG TAGCATACAG 
GGCAATTTGC TATGCCAGGC TTCTAAACTC AGTGGAAGTG ATCTGCTTCG TGGGTCTGGG 
ATGAGAAAAG GTGGCTCAAT GAATCACAAC AGCAATATGA CCCGGTCTCA AGATTTCTAC 
AACAAAGAAG GTCTAAGTGT GCGCCTTGGG CCAGCGGAGC AGTCTGACGC AGATGATGAA 
GAGGAGTCTG AACAGCTGAG CTCTCAGCAT AAAATTAAAG GTGGCAGAAA AAGACTTAAC 
AGCCAAGCTT CAGAGAATGT TGAGAAGCGA AGAAGTATCA ATCTAAGTGT ATGTGAGAGC 
CAGATATCAA AAAGCCAGTC AGATGAAGAG TCAACAAAGC TGAATACAGG GCAGATCTTT 
CCAAGTTCCA GTGGTCATCT TCCAAGCAGA CAGAATGGTG CACAACAGAG CAATTCTTTG 
ATTATGAATA TCCTTGGAGG TACTGGTGCT CTGAGGAACA TCTGGACTGA TCATCAAATT 
AGACAAGCCC TCTTTCCTTC TAGACGCTCT ACACAGGAAA ATGAGGAGGA TGATGATGAC 
TACCAGATGT TTCTGTTGTC TGAATCTGCA ACCAGGTTGG AATCGTCATG GTCACAGGAA 
GAAAGAGTGG CATCAAGCCG GCCATGTAGC TGGCACCTTG GTCTTATGGA AGAAGACACA 
ATACCAAACC CCTCCTGCAA AATTGTCAGG CGTGCAAGTA GTGTTGGAGA GAATAAATCT 
TGTTCTGCAA GACAACGTAG CCACTATCAT AAATCGGGGC CTCATGAACA TTTCAATCGT 
TCACATGAGC GTCATCAACA TTTTACTTGT CTTGGCTCTA ATGAGGACTT AACCATTGAT 
GACATTGAAC ATGTTTATGA CAATATTAGT TATGAAGATC TGAAACAGAT GGGGTTCACC 
AGGAGACATG ATTCTGATGC TCCTATAAAT AAGGAAGGAG GGGATTCTCA AAGCCAAGAA 
GACATTAGTA TTAGAAGACT AAGAGAACAG TTTGATAGAA CCAGGAACTC CCTAATCCTA 
AATAAAGAGA AAAATCTAAA GAAAGGAATG TCCAATTCAA ATTCACAAGA ACTCAGAATA 
ATTGAGGAAA ACATTTATGA CTCTATTCAG CTACCAGAAC AATCTCTGGA GCATGTAAAG 
CATAAATTGC ATTCTAAACG GAGCAATTTT TTGGCTCTGG GCACTGATTT TCAGTCTTGT 
GATCAGCTTG ATAGGTTTGT TTCCGAAGAC AGCCTACAGT TCAGCGAAGA TGAATCACTG 
GATCATCGTG TACCAGTGAA AGATGGAGAT TTCTGTTATT CCTCTTCAAA GGCTGGTTCA 
GTATCACATA AGTCTGCAGC AGATAAATTG TCAGAGGAAG TAGATGAAAT CTGGAATGAT 
TTGGAAAACT ACATTAAGAA GAATGAGGAG AAAAAAAGTG AACGGCTTTT AGCATCATTC 
CCTGTAAACA AAGATGACGT TCTTGAAAGG CGCCATGTAA CGTACATGCC TTGTTTCAGC 
AAAGATATGG AGTATTCATT GTCGTCATTG TGCTTGCCAG AAGATTTGCC TTCACAGAGG 
ACAATACTGA ATACTTCACA AATAAGTAAT GATAATAGTA GCTGTGGAAG CTCAAATAGT 
GGCTCATTAC TAAGTCTTAA GAGGACTTCC ATTGTAAGTG AAACAGGCTT TCTAGAATCA 
CCCCTTTTTG CTTCAAACAG CACTATTCCC TATTCCGAAG ATACATGTTC TTCTCAAACT 
TTAGAGAGCA CTGATAAATC AAAAAGCAAG GTTTTTATGA TGGCTCGCCA ATATAGTCAA 
AAAATAAAAA AAGCCAACCA GCTTTTAAAG ATGAAAAGCC CAGAACAAGA ACAGCCCTTT 
TACAAATCAC AGAAAATGAA ACCGAAGGAT CTTGCAGCTA TTATGGAAGA GAAGAAACAA 
GGAGGCTGTG CTATTGGTGC AAGAATTGCC GAGTATTCCC AGCTTTATGA CCAGATTATC 
TTTCGCGACT CCCCCATAAG GATGCAAAAG GAAGCAAAGA AAGACTCCCA CAAAGTAGCA 
TCTCCAAGTT ACTCTCCATT AAGCCCAACT TGCCAGCAAC CGTCCTCTCC ATCTAAATGG 
CAACAGCGAG GTAAATGTCT CCAATCAAAT AGCACTGGTT TACAGTCTGT TAATGGAAGT 
GGGGCAGAAT TTTATACATG GCCGGATGTA AAGGATTTTA AATCAAAAAC GGATTTATCA 
GAACCTGGCT CTGGGTGCCC TCACAGAAAC ATTACAGCTG CTTGTTCAGT GCCATCTTTG 
TCAAAGTCAT TACAGGATGC ATCGCATGAG TCAAGGTGGA GTGGCGTAAG CCAGCAGCAT 
AGTGAAAATG GACAAATGGT TTGTGCTTCT AATTCACCAG AAAGGATAGT GAATAAAGAA 
CCTCAGCTTT ATATTCGATC ACAGTCTTCA TCTAGTATGC TACATAACAG GACATCTGGC 
TCTCACAGAC TGGCAACAAA CTCACAGCGG TCAAAGCCCA TCTTAAAACC TACAAGGCCT 
ACAGATCTTG GTCATCTTGG ATATGAAAGA AAATCCCAAA TTGAAGACGA CACCTCTGAT 
CTTACCTTGC AAGATTCCCA GAAGGTTTTG GTCATGAACA CACTGTCCTC TCTTAATGCT 
CAAATAGCTA CGCTCAACTA TTATGCTAAC TTTAAGGATA CTGATGGAGA TGACGATGAT 
GATGACTATG TAGAGATCAA GTCAGAAGAT GAAGAGGATG AGTTAGAGAC TGATTCTCAT 
CAGGCTCTTA AAATGAGTGT AAAAGGAAAG CCAAATGTTA CTCAGACTTA CTGTAGTACA 
AATCCAACCT ATTCCTTTAG CGTGCCCAGT ACTCCAGTCA AGTCCTACAA CAGCAAGGAA 
CTCTCTGATG CACATATATT AGCCTATGAC GATGCAGACA AACTAAATGA CTATCTCTGG 
AGACCAACGC CTTCTAATCA GCAAAATATT GTTCAGTCAC TTAGAGAAAA ATTTCAGTGT 
CTCAGCTCAA GTAGTTTTGC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekhg1 ( XM_012963786.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819240.1 Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G1 (plekhg1), transcript variant X2, mRNA.

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CloneID	OXa34062
Clone ID Related Accession (Same CDS sequence)	XM_012963786.2 , XM_012963786.3
Accession Version	XM_012963786.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4653bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology domain-containing family G member 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963786.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTACTG AGGACTACCA TTTGCCTCTT TATGGTCTTC CATCTGTAAG AGAGCCTCAG 
AGTCCTAGGA CAGCAGAGGA AAATCTGCAA AGGACCAGCA GACATTTCCG TTACTGTGGG 
ATCAAAAACA TGGAGATGTC AGACAGTGAC CGGCCAGTCA GCTTTGGTTC AACATCCTCA 
TCTGCCTCTT CCAGAGACAG TCACTGTTCA TTTGGCAGTC GGATGACCCT AGCATCAAAC 
AGTCACTTGG GTTTGTTTCA CCAAGATAAG GAAGCTGGAG CCATAAAACT AGAACTGGTT 
CCTGCCCGGC AGATATCAAA TGATGACCTT CATAGGATCT GCACCAAAGA ACAACCTCAT 
GGAAGTCCAA GGGAAAAACC ATCTGATGAG CATCAGAGCA CACAGTGGAA AGCTGAAGCT 
AAAAGATCAA TTAAATGTTC AGTGACTGAA GCGGTAGAGA CAACCAGTCC TAAACTTATG 
TATGTAGACA GAGTAGTTCA AGAAATTCTC CATACTGAAA GGGCCTATGT GCAGGATCTG 
AAAAGCATTG TTGAGGATTA TCTCAATTGC ATTTCTGACC AATCAAGACT TTCCCTAGGG 
ACAGAAGAAC GGTCAGCTCT CTTTGGCAAT ATATGCGATA TCTACCATTT CAATAGTGAA 
CTTCTTCAAG AACTGGAAAA TTGTGATAAT GATCCTGTTG CAATAGCCGA ATGCTTTGTG 
TCAAAGAGTG AGGAATTCCA TATATACACA CAGTATTGTA CTAATTATCC AAGATCTGTT 
GCTATATTGA CAGAGTGTAT GAGAAACAAA ATACTGGCCA AATTCTTTAG AGAACGACAG 
GAAGTCCTAC AACATTCTCT TCCCTTAGGA TCTTACCTCT TGAAGCCTGT CCAGAGAATC 
CTTAAGTACC ACTTGCTGTT GCATGAAATA TCCAATCATT TGGATAAAGA CACTGAAGGC 
TATGACATTG TGCTGGATGC CATAGACACA ATGCAAAGGG TTGCATGGCA TATAAATGAT 
ATGAAGAGGA AACATGAACA TGACATTCGG CTCCAGGAAA TCCAGAGTTT GCTCACTAAT 
TGGAAAGGAC CAGATCTGAC AAGTTACGGG GAGCTAGTTT TGGAAGGAAC ATTCCGTTTT 
CAGAGGGCCA AGAATGAACG CACTTTGTTC CTGTTCGACC GTCTGCTTCT AATCACAAAG 
AAAAGGGATG ACACATACAC ATACAAGGCT CATATCCTGT GTTGCAATCT CATGCTAGTT 
GAAGTAATTC CAAAGGAGCC ACTTAGCTTC AGTGTCTTCC ATTATAAGAA CCCAAAGCTA 
CAGCACACAG TACAGGCAAA GTCACAGCAA GACAAGAGGC TCTGGATTTT GCATTTGAAG 
AGACTTATCC TTGAAAACCA CCCAGCGAAA ATCCCAGCAA AGGCTAAACA AGCAATTTTG 
GAAATGGATG CTATACATCA TCCCGGGTTC TCCTATACCC CAGAGGGAGA ACGGAAATCA 
CCTTTAAACT TGAAAGAAGA TCTCGCATCA TGCAGAGTCA GAAGAAAGTC AGAACCTTCG 
TCTAATCTAC ACAAAGTCAT GAAGCAAGAT GGTTTAAGCC CAGAGATGCA AAAGAGAAGT 
AGCATACAGG GCAATTTGCT ATGCCAGGCT TCTAAACTCA GTGGAAGTGA TCTGCTTCGT 
GGGTCTGGGA TGAGAAAAGG TGGCTCAATG AATCACAACA GCAATATGAC CCGGTCTCAA 
GATTTCTACA ACAAAGAAGG TCTAAGTGTG CGCCTTGGGC CAGCGGAGCA GTCTGACGCA 
GATGATGAAG AGGAGTCTGA ACAGCTGAGC TCTCAGCATA AAATTAAAGG TGGCAGAAAA 
AGACTTAACA GCCAAGCTTC AGAGAATGTT GAGAAGCGAA GAAGTATCAA TCTAAGTGTA 
TGTGAGAGCC AGATATCAAA AAGCCAGTCA GATGAAGAGT CAACAAAGCT GAATACAGGG 
CAGATCTTTC CAAGTTCCAG TGGTCATCTT CCAAGCAGAC AGAATGGTGC ACAACAGAGC 
AATTCTTTGA TTATGAATAT CCTTGGAGGT ACTGGTGCTC TGAGGAACAT CTGGACTGAT 
CATCAAATTA GACAAGCCCT CTTTCCTTCT AGACGCTCTA CACAGGAAAA TGAGGAGGAT 
GATGATGACT ACCAGATGTT TCTGTTGTCT GAATCTGCAA CCAGGTTGGA ATCGTCATGG 
TCACAGGAAG AAAGAGTGGC ATCAAGCCGG CCATGTAGCT GGCACCTTGG TCTTATGGAA 
GAAGACACAA TACCAAACCC CTCCTGCAAA ATTGTCAGGC GTGCAAGTAG TGTTGGAGAG 
AATAAATCTT GTTCTGCAAG ACAACGTAGC CACTATCATA AATCGGGGCC TCATGAACAT 
TTCAATCGTT CACATGAGCG TCATCAACAT TTTACTTGTC TTGGCTCTAA TGAGGACTTA 
ACCATTGATG ACATTGAACA TGTTTATGAC AATATTAGTT ATGAAGATCT GAAACAGATG 
GGGTTCACCA GGAGACATGA TTCTGATGCT CCTATAAATA AGGAAGGAGG GGATTCTCAA 
AGCCAAGAAG ACATTAGTAT TAGAAGACTA AGAGAACAGT TTGATAGAAC CAGGAACTCC 
CTAATCCTAA ATAAAGAGAA AAATCTAAAG AAAGGAATGT CCAATTCAAA TTCACAAGAA 
CTCAGAATAA TTGAGGAAAA CATTTATGAC TCTATTCAGC TACCAGAACA ATCTCTGGAG 
CATGTAAAGC ATAAATTGCA TTCTAAACGG AGCAATTTTT TGGCTCTGGG CACTGATTTT 
CAGTCTTGTG ATCAGCTTGA TAGGTTTGTT TCCGAAGACA GCCTACAGTT CAGCGAAGAT 
GAATCACTGG ATCATCGTGT ACCAGTGAAA GATGGAGATT TCTGTTATTC CTCTTCAAAG 
GCTGGTTCAG TATCACATAA GTCTGCAGCA GATAAATTGT CAGAGGAAGT AGATGAAATC 
TGGAATGATT TGGAAAACTA CATTAAGAAG AATGAGGAGA AAAAAAGTGA ACGGCTTTTA 
GCATCATTCC CTGTAAACAA AGATGACGTT CTTGAAAGGC GCCATGTAAC GTACATGCCT 
TGTTTCAGCA AAGATATGGA GTATTCATTG TCGTCATTGT GCTTGCCAGA AGATTTGCCT 
TCACAGAGGA CAATACTGAA TACTTCACAA ATAAGTAATG ATAATAGTAG CTGTGGAAGC 
TCAAATAGTG GCTCATTACT AAGTCTTAAG AGGACTTCCA TTGTAAGTGA AACAGGCTTT 
CTAGAATCAC CCCTTTTTGC TTCAAACAGC ACTATTCCCT ATTCCGAAGA TACATGTTCT 
TCTCAAACTT TAGAGAGCAC TGATAAATCA AAAAGCAAGG TTTTTATGAT GGCTCGCCAA 
TATAGTCAAA AAATAAAAAA AGCCAACCAG CTTTTAAAGA TGAAAAGCCC AGAACAAGAA 
CAGCCCTTTT ACAAATCACA GAAAATGAAA CCGAAGGATC TTGCAGCTAT TATGGAAGAG 
AAGAAACAAG GAGGCTGTGC TATTGGTGCA AGAATTGCCG AGTATTCCCA GCTTTATGAC 
CAGATTATCT TTCGCGACTC CCCCATAAGG ATGCAAAAGG AAGCAAAGAA AGACTCCCAC 
AAAGTAGCAT CTCCAAGTTA CTCTCCATTA AGCCCAACTT GCCAGCAACC GTCCTCTCCA 
TCTAAATGGC AACAGCGAGG TAAATGTCTC CAATCAAATA GCACTGGTTT ACAGTCTGTT 
AATGGAAGTG GGGCAGAATT TTATACATGG CCGGATGTAA AGGATTTTAA ATCAAAAACG 
GATTTATCAG AACCTGGCTC TGGGTGCCCT CACAGAAACA TTACAGCTGC TTGTTCAGTG 
CCATCTTTGT CAAAGTCATT ACAGGATGCA TCGCATGAGT CAAGGTGGAG TGGCGTAAGC 
CAGCAGCATA GTGAAAATGG ACAAATGGTT TGTGCTTCTA ATTCACCAGA AAGGATAGTG 
AATAAAGAAC CTCAGCTTTA TATTCGATCA CAGTCTTCAT CTAGTATGCT ACATAACAGG 
ACATCTGGCT CTCACAGACT GGCAACAAAC TCACAGCGGT CAAAGCCCAT CTTAAAACCT 
ACAAGGCCTA CAGATCTTGG TCATCTTGGA TATGAAAGAA AATCCCAAAT TGAAGACGAC 
ACCTCTGATC TTACCTTGCA AGATTCCCAG AAGGTTTTGG TCATGAACAC ACTGTCCTCT 
CTTAATGCTC AAATAGCTAC GCTCAACTAT TATGCTAACT TTAAGGATAC TGATGGAGAT 
GACGATGATG ATGACTATGT AGAGATCAAG TCAGAAGATG AAGAGGATGA GTTAGAGACT 
GATTCTCATC AGGCTCTTAA AATGAGTGTA AAAGGAAAGC CAAATGTTAC TCAGACTTAC 
TGTAGTACAA ATCCAACCTA TTCCTTTAGC GTGCCCAGTA CTCCAGTCAA GTCCTACAAC 
AGCAAGGAAC TCTCTGATGC ACATATATTA GCCTATGACG ATGCAGACAA ACTAAATGAC 
TATCTCTGGA GACCAACGCC TTCTAATCAG CAAAATATTG TTCAGTCACT TAGAGAAAAA 
TTTCAGTGTC TCAGCTCAAG TAGTTTTGCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819240.1
CDS	665..5317
Translation	MPTEDYHLPLYGLPSVREPQSPRTAEENLQRTSRHFRYCGIKNMEMSDSDRPVSFGSTSSSASSRDSHCSFGSRMTLASNSHLGLFHQDKEAGAIKLELVPARQISNDDLHRICTKEQPHGSPREKPSDEHQSTQWKAEAKRSIKCSVTEAVETTSPKLMYVDRVVQEILHTERAYVQDLKSIVEDYLNCISDQSRLSLGTEERSALFGNICDIYHFNSELLQELENCDNDPVAIAECFVSKSEEFHIYTQYCTNYPRSVAILTECMRNKILAKFFRERQEVLQHSLPLGSYLLKPVQRILKYHLLLHEISNHLDKDTEGYDIVLDAIDTMQRVAWHINDMKRKHEHDIRLQEIQSLLTNWKGPDLTSYGELVLEGTFRFQRAKNERTLFLFDRLLLITKKRDDTYTYKAHILCCNLMLVEVIPKEPLSFSVFHYKNPKLQHTVQAKSQQDKRLWILHLKRLILENHPAKIPAKAKQAILEMDAIHHPGFSYTPEGERKSPLNLKEDLASCRVRRKSEPSSNLHKVMKQDGLSPEMQKRSSIQGNLLCQASKLSGSDLLRGSGMRKGGSMNHNSNMTRSQDFYNKEGLSVRLGPAEQSDADDEEESEQLSSQHKIKGGRKRLNSQASENVEKRRSINLSVCESQISKSQSDEESTKLNTGQIFPSSSGHLPSRQNGAQQSNSLIMNILGGTGALRNIWTDHQIRQALFPSRRSTQENEEDDDDYQMFLLSESATRLESSWSQEERVASSRPCSWHLGLMEEDTIPNPSCKIVRRASSVGENKSCSARQRSHYHKSGPHEHFNRSHERHQHFTCLGSNEDLTIDDIEHVYDNISYEDLKQMGFTRRHDSDAPINKEGGDSQSQEDISIRRLREQFDRTRNSLILNKEKNLKKGMSNSNSQELRIIEENIYDSIQLPEQSLEHVKHKLHSKRSNFLALGTDFQSCDQLDRFVSEDSLQFSEDESLDHRVPVKDGDFCYSSSKAGSVSHKSAADKLSEEVDEIWNDLENYIKKNEEKKSERLLASFPVNKDDVLERRHVTYMPCFSKDMEYSLSSLCLPEDLPSQRTILNTSQISNDNSSCGSSNSGSLLSLKRTSIVSETGFLESPLFASNSTIPYSEDTCSSQTLESTDKSKSKVFMMARQYSQKIKKANQLLKMKSPEQEQPFYKSQKMKPKDLAAIMEEKKQGGCAIGARIAEYSQLYDQIIFRDSPIRMQKEAKKDSHKVASPSYSPLSPTCQQPSSPSKWQQRGKCLQSNSTGLQSVNGSGAEFYTWPDVKDFKSKTDLSEPGSGCPHRNITAACSVPSLSKSLQDASHESRWSGVSQQHSENGQMVCASNSPERIVNKEPQLYIRSQSSSSMLHNRTSGSHRLATNSQRSKPILKPTRPTDLGHLGYERKSQIEDDTSDLTLQDSQKVLVMNTLSSLNAQIATLNYYANFKDTDGDDDDDDYVEIKSEDEEDELETDSHQALKMSVKGKPNVTQTYCSTNPTYSFSVPSTPVKSYNSKELSDAHILAYDDADKLNDYLWRPTPSNQQNIVQSLREKFQCLSSSSFA

Target ORF information:

RefSeq Version	XM_012963786.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G1 (plekhg1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963786.2

ORF Insert Sequence:

ATGCCTACTG AGGACTACCA TTTGCCTCTT TATGGTCTTC CATCTGTAAG AGAGCCTCAG 
AGTCCTAGGA CAGCAGAGGA AAATCTGCAA AGGACCAGCA GACATTTCCG TTACTGTGGG 
ATCAAAAACA TGGAGATGTC AGACAGTGAC CGGCCAGTCA GCTTTGGTTC AACATCCTCA 
TCTGCCTCTT CCAGAGACAG TCACTGTTCA TTTGGCAGTC GGATGACCCT AGCATCAAAC 
AGTCACTTGG GTTTGTTTCA CCAAGATAAG GAAGCTGGAG CCATAAAACT AGAACTGGTT 
CCTGCCCGGC AGATATCAAA TGATGACCTT CATAGGATCT GCACCAAAGA ACAACCTCAT 
GGAAGTCCAA GGGAAAAACC ATCTGATGAG CATCAGAGCA CACAGTGGAA AGCTGAAGCT 
AAAAGATCAA TTAAATGTTC AGTGACTGAA GCGGTAGAGA CAACCAGTCC TAAACTTATG 
TATGTAGACA GAGTAGTTCA AGAAATTCTC CATACTGAAA GGGCCTATGT GCAGGATCTG 
AAAAGCATTG TTGAGGATTA TCTCAATTGC ATTTCTGACC AATCAAGACT TTCCCTAGGG 
ACAGAAGAAC GGTCAGCTCT CTTTGGCAAT ATATGCGATA TCTACCATTT CAATAGTGAA 
CTTCTTCAAG AACTGGAAAA TTGTGATAAT GATCCTGTTG CAATAGCCGA ATGCTTTGTG 
TCAAAGAGTG AGGAATTCCA TATATACACA CAGTATTGTA CTAATTATCC AAGATCTGTT 
GCTATATTGA CAGAGTGTAT GAGAAACAAA ATACTGGCCA AATTCTTTAG AGAACGACAG 
GAAGTCCTAC AACATTCTCT TCCCTTAGGA TCTTACCTCT TGAAGCCTGT CCAGAGAATC 
CTTAAGTACC ACTTGCTGTT GCATGAAATA TCCAATCATT TGGATAAAGA CACTGAAGGC 
TATGACATTG TGCTGGATGC CATAGACACA ATGCAAAGGG TTGCATGGCA TATAAATGAT 
ATGAAGAGGA AACATGAACA TGACATTCGG CTCCAGGAAA TCCAGAGTTT GCTCACTAAT 
TGGAAAGGAC CAGATCTGAC AAGTTACGGG GAGCTAGTTT TGGAAGGAAC ATTCCGTTTT 
CAGAGGGCCA AGAATGAACG CACTTTGTTC CTGTTCGACC GTCTGCTTCT AATCACAAAG 
AAAAGGGATG ACACATACAC ATACAAGGCT CATATCCTGT GTTGCAATCT CATGCTAGTT 
GAAGTAATTC CAAAGGAGCC ACTTAGCTTC AGTGTCTTCC ATTATAAGAA CCCAAAGCTA 
CAGCACACAG TACAGGCAAA GTCACAGCAA GACAAGAGGC TCTGGATTTT GCATTTGAAG 
AGACTTATCC TTGAAAACCA CCCAGCGAAA ATCCCAGCAA AGGCTAAACA AGCAATTTTG 
GAAATGGATG CTATACATCA TCCCGGGTTC TCCTATACCC CAGAGGGAGA ACGGAAATCA 
CCTTTAAACT TGAAAGAAGA TCTCGCATCA TGCAGAGTCA GAAGAAAGTC AGAACCTTCG 
TCTAATCTAC ACAAAGTCAT GAAGCAAGAT GGTTTAAGCC CAGAGATGCA AAAGAGAAGT 
AGCATACAGG GCAATTTGCT ATGCCAGGCT TCTAAACTCA GTGGAAGTGA TCTGCTTCGT 
GGGTCTGGGA TGAGAAAAGG TGGCTCAATG AATCACAACA GCAATATGAC CCGGTCTCAA 
GATTTCTACA ACAAAGAAGG TCTAAGTGTG CGCCTTGGGC CAGCGGAGCA GTCTGACGCA 
GATGATGAAG AGGAGTCTGA ACAGCTGAGC TCTCAGCATA AAATTAAAGG TGGCAGAAAA 
AGACTTAACA GCCAAGCTTC AGAGAATGTT GAGAAGCGAA GAAGTATCAA TCTAAGTGTA 
TGTGAGAGCC AGATATCAAA AAGCCAGTCA GATGAAGAGT CAACAAAGCT GAATACAGGG 
CAGATCTTTC CAAGTTCCAG TGGTCATCTT CCAAGCAGAC AGAATGGTGC ACAACAGAGC 
AATTCTTTGA TTATGAATAT CCTTGGAGGT ACTGGTGCTC TGAGGAACAT CTGGACTGAT 
CATCAAATTA GACAAGCCCT CTTTCCTTCT AGACGCTCTA CACAGGAAAA TGAGGAGGAT 
GATGATGACT ACCAGATGTT TCTGTTGTCT GAATCTGCAA CCAGGTTGGA ATCGTCATGG 
TCACAGGAAG AAAGAGTGGC ATCAAGCCGG CCATGTAGCT GGCACCTTGG TCTTATGGAA 
GAAGACACAA TACCAAACCC CTCCTGCAAA ATTGTCAGGC GTGCAAGTAG TGTTGGAGAG 
AATAAATCTT GTTCTGCAAG ACAACGTAGC CACTATCATA AATCGGGGCC TCATGAACAT 
TTCAATCGTT CACATGAGCG TCATCAACAT TTTACTTGTC TTGGCTCTAA TGAGGACTTA 
ACCATTGATG ACATTGAACA TGTTTATGAC AATATTAGTT ATGAAGATCT GAAACAGATG 
GGGTTCACCA GGAGACATGA TTCTGATGCT CCTATAAATA AGGAAGGAGG GGATTCTCAA 
AGCCAAGAAG ACATTAGTAT TAGAAGACTA AGAGAACAGT TTGATAGAAC CAGGAACTCC 
CTAATCCTAA ATAAAGAGAA AAATCTAAAG AAAGGAATGT CCAATTCAAA TTCACAAGAA 
CTCAGAATAA TTGAGGAAAA CATTTATGAC TCTATTCAGC TACCAGAACA ATCTCTGGAG 
CATGTAAAGC ATAAATTGCA TTCTAAACGG AGCAATTTTT TGGCTCTGGG CACTGATTTT 
CAGTCTTGTG ATCAGCTTGA TAGGTTTGTT TCCGAAGACA GCCTACAGTT CAGCGAAGAT 
GAATCACTGG ATCATCGTGT ACCAGTGAAA GATGGAGATT TCTGTTATTC CTCTTCAAAG 
GCTGGTTCAG TATCACATAA GTCTGCAGCA GATAAATTGT CAGAGGAAGT AGATGAAATC 
TGGAATGATT TGGAAAACTA CATTAAGAAG AATGAGGAGA AAAAAAGTGA ACGGCTTTTA 
GCATCATTCC CTGTAAACAA AGATGACGTT CTTGAAAGGC GCCATGTAAC GTACATGCCT 
TGTTTCAGCA AAGATATGGA GTATTCATTG TCGTCATTGT GCTTGCCAGA AGATTTGCCT 
TCACAGAGGA CAATACTGAA TACTTCACAA ATAAGTAATG ATAATAGTAG CTGTGGAAGC 
TCAAATAGTG GCTCATTACT AAGTCTTAAG AGGACTTCCA TTGTAAGTGA AACAGGCTTT 
CTAGAATCAC CCCTTTTTGC TTCAAACAGC ACTATTCCCT ATTCCGAAGA TACATGTTCT 
TCTCAAACTT TAGAGAGCAC TGATAAATCA AAAAGCAAGG TTTTTATGAT GGCTCGCCAA 
TATAGTCAAA AAATAAAAAA AGCCAACCAG CTTTTAAAGA TGAAAAGCCC AGAACAAGAA 
CAGCCCTTTT ACAAATCACA GAAAATGAAA CCGAAGGATC TTGCAGCTAT TATGGAAGAG 
AAGAAACAAG GAGGCTGTGC TATTGGTGCA AGAATTGCCG AGTATTCCCA GCTTTATGAC 
CAGATTATCT TTCGCGACTC CCCCATAAGG ATGCAAAAGG AAGCAAAGAA AGACTCCCAC 
AAAGTAGCAT CTCCAAGTTA CTCTCCATTA AGCCCAACTT GCCAGCAACC GTCCTCTCCA 
TCTAAATGGC AACAGCGAGG TAAATGTCTC CAATCAAATA GCACTGGTTT ACAGTCTGTT 
AATGGAAGTG GGGCAGAATT TTATACATGG CCGGATGTAA AGGATTTTAA ATCAAAAACG 
GATTTATCAG AACCTGGCTC TGGGTGCCCT CACAGAAACA TTACAGCTGC TTGTTCAGTG 
CCATCTTTGT CAAAGTCATT ACAGGATGCA TCGCATGAGT CAAGGTGGAG TGGCGTAAGC 
CAGCAGCATA GTGAAAATGG ACAAATGGTT TGTGCTTCTA ATTCACCAGA AAGGATAGTG 
AATAAAGAAC CTCAGCTTTA TATTCGATCA CAGTCTTCAT CTAGTATGCT ACATAACAGG 
ACATCTGGCT CTCACAGACT GGCAACAAAC TCACAGCGGT CAAAGCCCAT CTTAAAACCT 
ACAAGGCCTA CAGATCTTGG TCATCTTGGA TATGAAAGAA AATCCCAAAT TGAAGACGAC 
ACCTCTGATC TTACCTTGCA AGATTCCCAG AAGGTTTTGG TCATGAACAC ACTGTCCTCT 
CTTAATGCTC AAATAGCTAC GCTCAACTAT TATGCTAACT TTAAGGATAC TGATGGAGAT 
GACGATGATG ATGACTATGT AGAGATCAAG TCAGAAGATG AAGAGGATGA GTTAGAGACT 
GATTCTCATC AGGCTCTTAA AATGAGTGTA AAAGGAAAGC CAAATGTTAC TCAGACTTAC 
TGTAGTACAA ATCCAACCTA TTCCTTTAGC GTGCCCAGTA CTCCAGTCAA GTCCTACAAC 
AGCAAGGAAC TCTCTGATGC ACATATATTA GCCTATGACG ATGCAGACAA ACTAAATGAC 
TATCTCTGGA GACCAACGCC TTCTAATCAG CAAAATATTG TTCAGTCACT TAGAGAAAAA 
TTTCAGTGTC TCAGCTCAAG TAGTTTTGCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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plekhg1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

電話

FAX

オンラインフォーム

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