IDE cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	IDE
Entrez Gene ID	523752
Full Name	insulin degrading enzyme
General protein information	Preferred Names insulin degrading enzyme Names insulin-degrading enzyme insulin protease insulinase insulysin
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001075849.1	NP_001069317.1	insulin-degrading enzyme precursor
XM_010819775.3	XP_010818077.1	insulin-degrading enzyme isoform X1
XM_010819775.2	XP_010818077.1	insulin-degrading enzyme isoform X1
XM_010819776.2	XP_010818078.1	insulin-degrading enzyme isoform X2
XM_015460599.2	XP_015316085.1	insulin-degrading enzyme isoform X2
XM_015460599.1	XP_015316085.1	insulin-degrading enzyme isoform X2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	IDE	NP_001069317.1
Danio rerio (zebrafish)	ide	NP_001082994.1
Caenorhabditis elegans (roundworm)	CELE_Y70C5C.1	NP_507226.2
Caenorhabditis elegans (roundworm)	C28F5.4	NP_495575.2
Kluyveromyces lactis	KLLA0E05105g	XP_454175.1
Magnaporthe oryzae (rice blast fungus)	MGG_13149	XP_003710564.1
Arabidopsis thaliana (thale cress)	AT3G57470	NP_001118852.1
Xenopus tropicalis (tropical clawed frog)	ide	XP_002939138.2
Drosophila melanogaster (fruit fly)	Ide	NP_524182.3
Caenorhabditis elegans (roundworm)	C02G6.2	NP_504531.2
Saccharomyces cerevisiae (baker's yeast)	STE23	NP_013493.2
Neurospora crassa	NCU00481	XP_956166.1
Homo sapiens (human)	IDE	NP_004960.2
Pan troglodytes (chimpanzee)	IDE	XP_507922.2
	IDE	XP_001090249.1
Canis lupus familiaris (dog)	IDE	XP_534963.3
Mus musculus (house mouse)	Ide	NP_112419.3
Rattus norvegicus (Norway rat)	Ide	NP_037291.1
Gallus gallus (chicken)	IDE	XP_421686.4
Caenorhabditis elegans (roundworm)	F44E7.4	NP_741542.1
Caenorhabditis elegans (roundworm)	C02G6.1	NP_504532.1
Schizosaccharomyces pombe (fission yeast)	mug138	NP_593966.1
Oryza sativa (rice)	Os07g0570300	NP_001060044.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010351	XP_311589.4
Eremothecium gossypii	AGOS_AER053C	NP_984913.2
Arabidopsis thaliana (thale cress)	AT2G41790	NP_181710.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following IDE gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IDE cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa33467	XM_015460599.1 Latest version!	Bos taurus insulin-degrading enzyme (IDE), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa33467	XM_010819776.2 Latest version!	Bos taurus insulin-degrading enzyme (IDE), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa230877	XM_015460599.2 Latest version!	Bos taurus insulin degrading enzyme (IDE), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa230876	XM_010819775.3 Latest version!	Bos taurus insulin degrading enzyme (IDE), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa42699	NM_001075849.1 Latest version!	Bos taurus insulin degrading enzyme (IDE), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa50774	XM_010819775.2 Latest version!	Bos taurus insulin-degrading enzyme (IDE), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

IDE ( XM_010819776.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010818078.1 Bos taurus insulin-degrading enzyme (IDE), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa33467
Clone ID Related Accession (Same CDS sequence)	XM_010819776.2 , XM_015460599.1
Accession Version	XM_010819776.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2937bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	insulin-degrading enzyme isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104581.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010819776.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATAATC CAGCAATCAA GAGAATAGGA CATCATATCA TCAAGTCTCA TGAAGACAAG 
CGAGAGTACC GCGGACTTGA GCTGGCCAAT GGGATCAAAG TACTTCTTGT CAGTGATCCA 
ACAACAGATA AATCATCAGC AGCACTTGAT GTACACATAG GTTCATTGTC AGATCCTCCA 
AATATTGCTG GCTTAAGTCA TTTTTGTGAG CATATGTTAT TTTTGGGAAC AAAGAAATAC 
CCTAAAGAAA ATGAATACAG CCAGTTTCTC AGTGAACATG CAGGAAGTTC AAACGCCTTC 
ACTAGTGGAG AGCATACCAA TTACTATTTT GATGTTTCTC ATGAACACCT AGAAGGTGCG 
CTAGACAGAT TTGCACAGTT TTTCCTGTGC CCCTTGTTTG ATGAGAGCTG CAAAGACAGA 
GAGGTGAATG CAGTTGATTC AGAACATGAG AAGAATGTGA TGAATGATGC CTGGAGACTC 
TTTCAGCTGG AAAAAGCTAC AGGGAATCCC AAACATCCCT TCAGTAAATT TGGAACAGGT 
AACAAATATA CTCTAGAGAC TAGACCGAAC CAAGAAGGCA TTGATGTAAG ACAAGAGCTC 
CTGAAATTCC ATTCTACTTA TTATTCATCG AACTTAATGG CTATTTGTGT TTTAGGTCGA 
GAATCTTTGG ATGATTTGAC TAATCTGGTG GTAAAGTTAT TTTCTGAAGT AGAGAACAAA 
AATGTCCCGT TGCCAGAATT TCCTGAACAT CCTTTCCAAG AAGAACATCT TAAACAACTT 
TACAAAATAG TACCCATTAA GGATATTAGG AATCTTTATG TGACATTTCC CATACCTGAC 
CTTCAGAAAT ACTACAAATC AAATCCCGGT CATTATCTCG GTCATCTCAT TGGGCATGAA 
GGTCCTGGAA GTCTGTTATC AGAACTTAAA TCAAAGGGCT GGGTAAATAC TCTTGTTGGT 
GGGCAAAAGG AAGGAGCCCG AGGTTTTATG TTTTTTATCA TTAATGTGGA CTTGACTGAG 
GAAGGATTAT TACATGTTGA GGATATAATT TTGCACATGT TTCAATACAT TCAGAAGTTA 
CGAGCAGAAG GACCTCAAGA ATGGGTTTTC CAAGAGTGCA AGGACTTGAA TGCTGTTGCT 
TTTAGGTTTA AAGATAAAGA GAGACCACGG GGTTATACCT CTAAGATTGC AGGAATATTA 
CATTATTATC CCCTAGAAGA AGTGCTCACA GCTGAATATT TACTGGAAGA ATTTAGACCA 
GATTTAATAG AGATGGTTCT CGATAAACTC AGACCAGAAA ATGTCCGGGT TGCAATAGTT 
TCCAAATCTT TTGAAGGAAA AACTGATCGC ACAGAAGAGT GGTACGGAAC CCAGTACAAA 
CAAGAAGCTA TACCAGACGA AGTCATTAAG AAATGGCAAA ATGCTGATCT GAATGGGAAA 
TTTAAACTTC CAATGAAGAA TGAATTTATT CCTACAAATT TTGAAATTTT ATCATTAGAA 
AAAGAAGCAA CGCCATACCC TTCTCTTATT AAGGATACAG CTATGAGCAA ACTCTGGTTC 
AAACAAGATG ATAAGTTTTT CTTGCCCAAG GCCTGTCTCA ACTTTGAATT TTTCAGCCCA 
TTTGCTTATG TGGACCCCTT GCACTGTAAC ATGGCCTATT TGTACCTTGA GCTCCTCAAA 
GACTCACTCA ACGAGTATGC ATATGCAGCA GAGCTAGCAG GCTTGAGCTA TGACCTCCAA 
AATACCATCT ATGGGATGTA TCTCTCGGTG AAAGGTTACA ATGACAAGCA GCCAATTTTG 
CTAAAGAAGA TCATTGAGAA AATGGCTACC TTTGAGATTG ATGAAAAAAG ATTTGAAATT 
ATCAAAGAGG CATATATGCG ATCTCTTAAC AATTTCCGAG CTGAACAGCC TCATCAACAC 
GCCATGTACT ACCTCCGCTT GCTGATGACT GAAGTGGCCT GGACTAAAGA TGAATTAAAA 
GAAGCCTTGG ATGATGTCAC CCTTCCTCGC CTTAAGGCCT TCATACCTCA ACTCCTGTCA 
CGGCTGCATA TTGAAGCCCT TCTCCATGGA AATATAACAA AGCAGGCTGC GTTAGGAATC 
ATGCAGATGG TTGAAGACAC CCTTATTGAA CATGCTCATA CGAAACCACT TCTTCCAAGT 
CAGCTCGTTC GGTACAGAGA AGTTCAGCTC CCGGACAGGG GATGGTTTGT GTATCAGCAG 
AGGAATGAAG TCCACAATAA CTGTGGCATT GAGATATACT ACCAAACCGA CATGCAGAGC 
ACGTCAGAGA ACATGTTTCT GGAGCTCTTC TGTCAGATCA TCTCCGAGCC TTGCTTCAAC 
ACCCTGCGCA CCAAGGAGCA ACTGGGCTAT ATCGTCTTCA GCGGGCCACG CCGAGCCAAT 
GGCATTCAGG GCTTACGGTT TATCATCCAG TCAGAAAAGC CACCTCACTA CCTAGAAAGC 
AGAGTGGAAG CTTTCTTAAT TACCATGGAA AAGTCCATAG AGGACATGAC AGAAGAGGCC 
TTCCAAAAAC ACATCCAAGC ATTAGCAATC CGTCGACTAG ACAAACCAAA GAAGCTATCT 
GCAGAGTGTG CTAAATATTG GGGGGAGATC ATCTCTCAGC AGTACAATTT TGACAGAGAT 
AATATAGAAG TTGCCTATTT GAAGACACTT ACCAAGGAAG ATATTATCAA ATTCTATAAG 
GAAATGTTGG CGGTAGATGC CCCGAGGAGA CACAAGGTAT CCGTCCATGT TCTTGCCAGA 
GAAATGGATT CTTGTCCTGT GGTTGGAGAG TTCCCATGTC AAAATGATAT AAATTTGTCA 
CAAGCACCAG CCTTGCCACA ACCTGAAGTG ATTCAGAACA TGACTGAGTT CAAGCGTGGT 
TTGCCACTGT TCCCCCTTGT GAAACCACAT ATTAACTTCA TGGCTGCAAA ACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010818078.1
CDS	45..2981
Translation	MNNPAIKRIGHHIIKSHEDKREYRGLELANGIKVLLVSDPTTDKSSAALDVHIGSLSDPPNIAGLSHFCEHMLFLGTKKYPKENEYSQFLSEHAGSSNAFTSGEHTNYYFDVSHEHLEGALDRFAQFFLCPLFDESCKDREVNAVDSEHEKNVMNDAWRLFQLEKATGNPKHPFSKFGTGNKYTLETRPNQEGIDVRQELLKFHSTYYSSNLMAICVLGRESLDDLTNLVVKLFSEVENKNVPLPEFPEHPFQEEHLKQLYKIVPIKDIRNLYVTFPIPDLQKYYKSNPGHYLGHLIGHEGPGSLLSELKSKGWVNTLVGGQKEGARGFMFFIINVDLTEEGLLHVEDIILHMFQYIQKLRAEGPQEWVFQECKDLNAVAFRFKDKERPRGYTSKIAGILHYYPLEEVLTAEYLLEEFRPDLIEMVLDKLRPENVRVAIVSKSFEGKTDRTEEWYGTQYKQEAIPDEVIKKWQNADLNGKFKLPMKNEFIPTNFEILSLEKEATPYPSLIKDTAMSKLWFKQDDKFFLPKACLNFEFFSPFAYVDPLHCNMAYLYLELLKDSLNEYAYAAELAGLSYDLQNTIYGMYLSVKGYNDKQPILLKKIIEKMATFEIDEKRFEIIKEAYMRSLNNFRAEQPHQHAMYYLRLLMTEVAWTKDELKEALDDVTLPRLKAFIPQLLSRLHIEALLHGNITKQAALGIMQMVEDTLIEHAHTKPLLPSQLVRYREVQLPDRGWFVYQQRNEVHNNCGIEIYYQTDMQSTSENMFLELFCQIISEPCFNTLRTKEQLGYIVFSGPRRANGIQGLRFIIQSEKPPHYLESRVEAFLITMEKSIEDMTEEAFQKHIQALAIRRLDKPKKLSAECAKYWGEIISQQYNFDRDNIEVAYLKTLTKEDIIKFYKEMLAVDAPRRHKVSVHVLAREMDSCPVVGEFPCQNDINLSQAPALPQPEVIQNMTEFKRGLPLFPLVKPHINFMAAKL

Target ORF information:

RefSeq Version	XM_010819776.2
Organism	Bos taurus(cattle)
Definition	Bos taurus insulin-degrading enzyme (IDE), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010819776.2

ORF Insert Sequence:

ATGAATAATC CAGCAATCAA GAGAATAGGA CATCATATCA TCAAGTCTCA TGAAGACAAG 
CGAGAGTACC GCGGACTTGA GCTGGCCAAT GGGATCAAAG TACTTCTTGT CAGTGATCCA 
ACAACAGATA AATCATCAGC AGCACTTGAT GTACACATAG GTTCATTGTC AGATCCTCCA 
AATATTGCTG GCTTAAGTCA TTTTTGTGAG CATATGTTAT TTTTGGGAAC AAAGAAATAC 
CCTAAAGAAA ATGAATACAG CCAGTTTCTC AGTGAACATG CAGGAAGTTC AAACGCCTTC 
ACTAGTGGAG AGCATACCAA TTACTATTTT GATGTTTCTC ATGAACACCT AGAAGGTGCG 
CTAGACAGAT TTGCACAGTT TTTCCTGTGC CCCTTGTTTG ATGAGAGCTG CAAAGACAGA 
GAGGTGAATG CAGTTGATTC AGAACATGAG AAGAATGTGA TGAATGATGC CTGGAGACTC 
TTTCAGCTGG AAAAAGCTAC AGGGAATCCC AAACATCCCT TCAGTAAATT TGGAACAGGT 
AACAAATATA CTCTAGAGAC TAGACCGAAC CAAGAAGGCA TTGATGTAAG ACAAGAGCTC 
CTGAAATTCC ATTCTACTTA TTATTCATCG AACTTAATGG CTATTTGTGT TTTAGGTCGA 
GAATCTTTGG ATGATTTGAC TAATCTGGTG GTAAAGTTAT TTTCTGAAGT AGAGAACAAA 
AATGTCCCGT TGCCAGAATT TCCTGAACAT CCTTTCCAAG AAGAACATCT TAAACAACTT 
TACAAAATAG TACCCATTAA GGATATTAGG AATCTTTATG TGACATTTCC CATACCTGAC 
CTTCAGAAAT ACTACAAATC AAATCCCGGT CATTATCTCG GTCATCTCAT TGGGCATGAA 
GGTCCTGGAA GTCTGTTATC AGAACTTAAA TCAAAGGGCT GGGTAAATAC TCTTGTTGGT 
GGGCAAAAGG AAGGAGCCCG AGGTTTTATG TTTTTTATCA TTAATGTGGA CTTGACTGAG 
GAAGGATTAT TACATGTTGA GGATATAATT TTGCACATGT TTCAATACAT TCAGAAGTTA 
CGAGCAGAAG GACCTCAAGA ATGGGTTTTC CAAGAGTGCA AGGACTTGAA TGCTGTTGCT 
TTTAGGTTTA AAGATAAAGA GAGACCACGG GGTTATACCT CTAAGATTGC AGGAATATTA 
CATTATTATC CCCTAGAAGA AGTGCTCACA GCTGAATATT TACTGGAAGA ATTTAGACCA 
GATTTAATAG AGATGGTTCT CGATAAACTC AGACCAGAAA ATGTCCGGGT TGCAATAGTT 
TCCAAATCTT TTGAAGGAAA AACTGATCGC ACAGAAGAGT GGTACGGAAC CCAGTACAAA 
CAAGAAGCTA TACCAGACGA AGTCATTAAG AAATGGCAAA ATGCTGATCT GAATGGGAAA 
TTTAAACTTC CAATGAAGAA TGAATTTATT CCTACAAATT TTGAAATTTT ATCATTAGAA 
AAAGAAGCAA CGCCATACCC TTCTCTTATT AAGGATACAG CTATGAGCAA ACTCTGGTTC 
AAACAAGATG ATAAGTTTTT CTTGCCCAAG GCCTGTCTCA ACTTTGAATT TTTCAGCCCA 
TTTGCTTATG TGGACCCCTT GCACTGTAAC ATGGCCTATT TGTACCTTGA GCTCCTCAAA 
GACTCACTCA ACGAGTATGC ATATGCAGCA GAGCTAGCAG GCTTGAGCTA TGACCTCCAA 
AATACCATCT ATGGGATGTA TCTCTCGGTG AAAGGTTACA ATGACAAGCA GCCAATTTTG 
CTAAAGAAGA TCATTGAGAA AATGGCTACC TTTGAGATTG ATGAAAAAAG ATTTGAAATT 
ATCAAAGAGG CATATATGCG ATCTCTTAAC AATTTCCGAG CTGAACAGCC TCATCAACAC 
GCCATGTACT ACCTCCGCTT GCTGATGACT GAAGTGGCCT GGACTAAAGA TGAATTAAAA 
GAAGCCTTGG ATGATGTCAC CCTTCCTCGC CTTAAGGCCT TCATACCTCA ACTCCTGTCA 
CGGCTGCATA TTGAAGCCCT TCTCCATGGA AATATAACAA AGCAGGCTGC GTTAGGAATC 
ATGCAGATGG TTGAAGACAC CCTTATTGAA CATGCTCATA CGAAACCACT TCTTCCAAGT 
CAGCTCGTTC GGTACAGAGA AGTTCAGCTC CCGGACAGGG GATGGTTTGT GTATCAGCAG 
AGGAATGAAG TCCACAATAA CTGTGGCATT GAGATATACT ACCAAACCGA CATGCAGAGC 
ACGTCAGAGA ACATGTTTCT GGAGCTCTTC TGTCAGATCA TCTCCGAGCC TTGCTTCAAC 
ACCCTGCGCA CCAAGGAGCA ACTGGGCTAT ATCGTCTTCA GCGGGCCACG CCGAGCCAAT 
GGCATTCAGG GCTTACGGTT TATCATCCAG TCAGAAAAGC CACCTCACTA CCTAGAAAGC 
AGAGTGGAAG CTTTCTTAAT TACCATGGAA AAGTCCATAG AGGACATGAC AGAAGAGGCC 
TTCCAAAAAC ACATCCAAGC ATTAGCAATC CGTCGACTAG ACAAACCAAA GAAGCTATCT 
GCAGAGTGTG CTAAATATTG GGGGGAGATC ATCTCTCAGC AGTACAATTT TGACAGAGAT 
AATATAGAAG TTGCCTATTT GAAGACACTT ACCAAGGAAG ATATTATCAA ATTCTATAAG 
GAAATGTTGG CGGTAGATGC CCCGAGGAGA CACAAGGTAT CCGTCCATGT TCTTGCCAGA 
GAAATGGATT CTTGTCCTGT GGTTGGAGAG TTCCCATGTC AAAATGATAT AAATTTGTCA 
CAAGCACCAG CCTTGCCACA ACCTGAAGTG ATTCAGAACA TGACTGAGTT CAAGCGTGGT 
TTGCCACTGT TCCCCCTTGT GAAACCACAT ATTAACTTCA TGGCTGCAAA ACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IDE ( XM_015460599.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015316085.1 Bos taurus insulin-degrading enzyme (IDE), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa33467
Clone ID Related Accession (Same CDS sequence)	XM_010819776.2 , XM_015460599.1
Accession Version	XM_015460599.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2937bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	insulin-degrading enzyme isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104581.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATAATC CAGCAATCAA GAGAATAGGA CATCATATCA TCAAGTCTCA TGAAGACAAG 
CGAGAGTACC GCGGACTTGA GCTGGCCAAT GGGATCAAAG TACTTCTTGT CAGTGATCCA 
ACAACAGATA AATCATCAGC AGCACTTGAT GTACACATAG GTTCATTGTC AGATCCTCCA 
AATATTGCTG GCTTAAGTCA TTTTTGTGAG CATATGTTAT TTTTGGGAAC AAAGAAATAC 
CCTAAAGAAA ATGAATACAG CCAGTTTCTC AGTGAACATG CAGGAAGTTC AAACGCCTTC 
ACTAGTGGAG AGCATACCAA TTACTATTTT GATGTTTCTC ATGAACACCT AGAAGGTGCG 
CTAGACAGAT TTGCACAGTT TTTCCTGTGC CCCTTGTTTG ATGAGAGCTG CAAAGACAGA 
GAGGTGAATG CAGTTGATTC AGAACATGAG AAGAATGTGA TGAATGATGC CTGGAGACTC 
TTTCAGCTGG AAAAAGCTAC AGGGAATCCC AAACATCCCT TCAGTAAATT TGGAACAGGT 
AACAAATATA CTCTAGAGAC TAGACCGAAC CAAGAAGGCA TTGATGTAAG ACAAGAGCTC 
CTGAAATTCC ATTCTACTTA TTATTCATCG AACTTAATGG CTATTTGTGT TTTAGGTCGA 
GAATCTTTGG ATGATTTGAC TAATCTGGTG GTAAAGTTAT TTTCTGAAGT AGAGAACAAA 
AATGTCCCGT TGCCAGAATT TCCTGAACAT CCTTTCCAAG AAGAACATCT TAAACAACTT 
TACAAAATAG TACCCATTAA GGATATTAGG AATCTTTATG TGACATTTCC CATACCTGAC 
CTTCAGAAAT ACTACAAATC AAATCCCGGT CATTATCTCG GTCATCTCAT TGGGCATGAA 
GGTCCTGGAA GTCTGTTATC AGAACTTAAA TCAAAGGGCT GGGTAAATAC TCTTGTTGGT 
GGGCAAAAGG AAGGAGCCCG AGGTTTTATG TTTTTTATCA TTAATGTGGA CTTGACTGAG 
GAAGGATTAT TACATGTTGA GGATATAATT TTGCACATGT TTCAATACAT TCAGAAGTTA 
CGAGCAGAAG GACCTCAAGA ATGGGTTTTC CAAGAGTGCA AGGACTTGAA TGCTGTTGCT 
TTTAGGTTTA AAGATAAAGA GAGACCACGG GGTTATACCT CTAAGATTGC AGGAATATTA 
CATTATTATC CCCTAGAAGA AGTGCTCACA GCTGAATATT TACTGGAAGA ATTTAGACCA 
GATTTAATAG AGATGGTTCT CGATAAACTC AGACCAGAAA ATGTCCGGGT TGCAATAGTT 
TCCAAATCTT TTGAAGGAAA AACTGATCGC ACAGAAGAGT GGTACGGAAC CCAGTACAAA 
CAAGAAGCTA TACCAGACGA AGTCATTAAG AAATGGCAAA ATGCTGATCT GAATGGGAAA 
TTTAAACTTC CAATGAAGAA TGAATTTATT CCTACAAATT TTGAAATTTT ATCATTAGAA 
AAAGAAGCAA CGCCATACCC TTCTCTTATT AAGGATACAG CTATGAGCAA ACTCTGGTTC 
AAACAAGATG ATAAGTTTTT CTTGCCCAAG GCCTGTCTCA ACTTTGAATT TTTCAGCCCA 
TTTGCTTATG TGGACCCCTT GCACTGTAAC ATGGCCTATT TGTACCTTGA GCTCCTCAAA 
GACTCACTCA ACGAGTATGC ATATGCAGCA GAGCTAGCAG GCTTGAGCTA TGACCTCCAA 
AATACCATCT ATGGGATGTA TCTCTCGGTG AAAGGTTACA ATGACAAGCA GCCAATTTTG 
CTAAAGAAGA TCATTGAGAA AATGGCTACC TTTGAGATTG ATGAAAAAAG ATTTGAAATT 
ATCAAAGAGG CATATATGCG ATCTCTTAAC AATTTCCGAG CTGAACAGCC TCATCAACAC 
GCCATGTACT ACCTCCGCTT GCTGATGACT GAAGTGGCCT GGACTAAAGA TGAATTAAAA 
GAAGCCTTGG ATGATGTCAC CCTTCCTCGC CTTAAGGCCT TCATACCTCA ACTCCTGTCA 
CGGCTGCATA TTGAAGCCCT TCTCCATGGA AATATAACAA AGCAGGCTGC GTTAGGAATC 
ATGCAGATGG TTGAAGACAC CCTTATTGAA CATGCTCATA CGAAACCACT TCTTCCAAGT 
CAGCTCGTTC GGTACAGAGA AGTTCAGCTC CCGGACAGGG GATGGTTTGT GTATCAGCAG 
AGGAATGAAG TCCACAATAA CTGTGGCATT GAGATATACT ACCAAACCGA CATGCAGAGC 
ACGTCAGAGA ACATGTTTCT GGAGCTCTTC TGTCAGATCA TCTCCGAGCC TTGCTTCAAC 
ACCCTGCGCA CCAAGGAGCA ACTGGGCTAT ATCGTCTTCA GCGGGCCACG CCGAGCCAAT 
GGCATTCAGG GCTTACGGTT TATCATCCAG TCAGAAAAGC CACCTCACTA CCTAGAAAGC 
AGAGTGGAAG CTTTCTTAAT TACCATGGAA AAGTCCATAG AGGACATGAC AGAAGAGGCC 
TTCCAAAAAC ACATCCAAGC ATTAGCAATC CGTCGACTAG ACAAACCAAA GAAGCTATCT 
GCAGAGTGTG CTAAATATTG GGGGGAGATC ATCTCTCAGC AGTACAATTT TGACAGAGAT 
AATATAGAAG TTGCCTATTT GAAGACACTT ACCAAGGAAG ATATTATCAA ATTCTATAAG 
GAAATGTTGG CGGTAGATGC CCCGAGGAGA CACAAGGTAT CCGTCCATGT TCTTGCCAGA 
GAAATGGATT CTTGTCCTGT GGTTGGAGAG TTCCCATGTC AAAATGATAT AAATTTGTCA 
CAAGCACCAG CCTTGCCACA ACCTGAAGTG ATTCAGAACA TGACTGAGTT CAAGCGTGGT 
TTGCCACTGT TCCCCCTTGT GAAACCACAT ATTAACTTCA TGGCTGCAAA ACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015316085.1
CDS	303..3239
Translation	MNNPAIKRIGHHIIKSHEDKREYRGLELANGIKVLLVSDPTTDKSSAALDVHIGSLSDPPNIAGLSHFCEHMLFLGTKKYPKENEYSQFLSEHAGSSNAFTSGEHTNYYFDVSHEHLEGALDRFAQFFLCPLFDESCKDREVNAVDSEHEKNVMNDAWRLFQLEKATGNPKHPFSKFGTGNKYTLETRPNQEGIDVRQELLKFHSTYYSSNLMAICVLGRESLDDLTNLVVKLFSEVENKNVPLPEFPEHPFQEEHLKQLYKIVPIKDIRNLYVTFPIPDLQKYYKSNPGHYLGHLIGHEGPGSLLSELKSKGWVNTLVGGQKEGARGFMFFIINVDLTEEGLLHVEDIILHMFQYIQKLRAEGPQEWVFQECKDLNAVAFRFKDKERPRGYTSKIAGILHYYPLEEVLTAEYLLEEFRPDLIEMVLDKLRPENVRVAIVSKSFEGKTDRTEEWYGTQYKQEAIPDEVIKKWQNADLNGKFKLPMKNEFIPTNFEILSLEKEATPYPSLIKDTAMSKLWFKQDDKFFLPKACLNFEFFSPFAYVDPLHCNMAYLYLELLKDSLNEYAYAAELAGLSYDLQNTIYGMYLSVKGYNDKQPILLKKIIEKMATFEIDEKRFEIIKEAYMRSLNNFRAEQPHQHAMYYLRLLMTEVAWTKDELKEALDDVTLPRLKAFIPQLLSRLHIEALLHGNITKQAALGIMQMVEDTLIEHAHTKPLLPSQLVRYREVQLPDRGWFVYQQRNEVHNNCGIEIYYQTDMQSTSENMFLELFCQIISEPCFNTLRTKEQLGYIVFSGPRRANGIQGLRFIIQSEKPPHYLESRVEAFLITMEKSIEDMTEEAFQKHIQALAIRRLDKPKKLSAECAKYWGEIISQQYNFDRDNIEVAYLKTLTKEDIIKFYKEMLAVDAPRRHKVSVHVLAREMDSCPVVGEFPCQNDINLSQAPALPQPEVIQNMTEFKRGLPLFPLVKPHINFMAAKL

Target ORF information:

RefSeq Version	XM_015460599.1
Organism	Bos taurus(cattle)
Definition	Bos taurus insulin-degrading enzyme (IDE), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015460599.1

ORF Insert Sequence:

ATGAATAATC CAGCAATCAA GAGAATAGGA CATCATATCA TCAAGTCTCA TGAAGACAAG 
CGAGAGTACC GCGGACTTGA GCTGGCCAAT GGGATCAAAG TACTTCTTGT CAGTGATCCA 
ACAACAGATA AATCATCAGC AGCACTTGAT GTACACATAG GTTCATTGTC AGATCCTCCA 
AATATTGCTG GCTTAAGTCA TTTTTGTGAG CATATGTTAT TTTTGGGAAC AAAGAAATAC 
CCTAAAGAAA ATGAATACAG CCAGTTTCTC AGTGAACATG CAGGAAGTTC AAACGCCTTC 
ACTAGTGGAG AGCATACCAA TTACTATTTT GATGTTTCTC ATGAACACCT AGAAGGTGCG 
CTAGACAGAT TTGCACAGTT TTTCCTGTGC CCCTTGTTTG ATGAGAGCTG CAAAGACAGA 
GAGGTGAATG CAGTTGATTC AGAACATGAG AAGAATGTGA TGAATGATGC CTGGAGACTC 
TTTCAGCTGG AAAAAGCTAC AGGGAATCCC AAACATCCCT TCAGTAAATT TGGAACAGGT 
AACAAATATA CTCTAGAGAC TAGACCGAAC CAAGAAGGCA TTGATGTAAG ACAAGAGCTC 
CTGAAATTCC ATTCTACTTA TTATTCATCG AACTTAATGG CTATTTGTGT TTTAGGTCGA 
GAATCTTTGG ATGATTTGAC TAATCTGGTG GTAAAGTTAT TTTCTGAAGT AGAGAACAAA 
AATGTCCCGT TGCCAGAATT TCCTGAACAT CCTTTCCAAG AAGAACATCT TAAACAACTT 
TACAAAATAG TACCCATTAA GGATATTAGG AATCTTTATG TGACATTTCC CATACCTGAC 
CTTCAGAAAT ACTACAAATC AAATCCCGGT CATTATCTCG GTCATCTCAT TGGGCATGAA 
GGTCCTGGAA GTCTGTTATC AGAACTTAAA TCAAAGGGCT GGGTAAATAC TCTTGTTGGT 
GGGCAAAAGG AAGGAGCCCG AGGTTTTATG TTTTTTATCA TTAATGTGGA CTTGACTGAG 
GAAGGATTAT TACATGTTGA GGATATAATT TTGCACATGT TTCAATACAT TCAGAAGTTA 
CGAGCAGAAG GACCTCAAGA ATGGGTTTTC CAAGAGTGCA AGGACTTGAA TGCTGTTGCT 
TTTAGGTTTA AAGATAAAGA GAGACCACGG GGTTATACCT CTAAGATTGC AGGAATATTA 
CATTATTATC CCCTAGAAGA AGTGCTCACA GCTGAATATT TACTGGAAGA ATTTAGACCA 
GATTTAATAG AGATGGTTCT CGATAAACTC AGACCAGAAA ATGTCCGGGT TGCAATAGTT 
TCCAAATCTT TTGAAGGAAA AACTGATCGC ACAGAAGAGT GGTACGGAAC CCAGTACAAA 
CAAGAAGCTA TACCAGACGA AGTCATTAAG AAATGGCAAA ATGCTGATCT GAATGGGAAA 
TTTAAACTTC CAATGAAGAA TGAATTTATT CCTACAAATT TTGAAATTTT ATCATTAGAA 
AAAGAAGCAA CGCCATACCC TTCTCTTATT AAGGATACAG CTATGAGCAA ACTCTGGTTC 
AAACAAGATG ATAAGTTTTT CTTGCCCAAG GCCTGTCTCA ACTTTGAATT TTTCAGCCCA 
TTTGCTTATG TGGACCCCTT GCACTGTAAC ATGGCCTATT TGTACCTTGA GCTCCTCAAA 
GACTCACTCA ACGAGTATGC ATATGCAGCA GAGCTAGCAG GCTTGAGCTA TGACCTCCAA 
AATACCATCT ATGGGATGTA TCTCTCGGTG AAAGGTTACA ATGACAAGCA GCCAATTTTG 
CTAAAGAAGA TCATTGAGAA AATGGCTACC TTTGAGATTG ATGAAAAAAG ATTTGAAATT 
ATCAAAGAGG CATATATGCG ATCTCTTAAC AATTTCCGAG CTGAACAGCC TCATCAACAC 
GCCATGTACT ACCTCCGCTT GCTGATGACT GAAGTGGCCT GGACTAAAGA TGAATTAAAA 
GAAGCCTTGG ATGATGTCAC CCTTCCTCGC CTTAAGGCCT TCATACCTCA ACTCCTGTCA 
CGGCTGCATA TTGAAGCCCT TCTCCATGGA AATATAACAA AGCAGGCTGC GTTAGGAATC 
ATGCAGATGG TTGAAGACAC CCTTATTGAA CATGCTCATA CGAAACCACT TCTTCCAAGT 
CAGCTCGTTC GGTACAGAGA AGTTCAGCTC CCGGACAGGG GATGGTTTGT GTATCAGCAG 
AGGAATGAAG TCCACAATAA CTGTGGCATT GAGATATACT ACCAAACCGA CATGCAGAGC 
ACGTCAGAGA ACATGTTTCT GGAGCTCTTC TGTCAGATCA TCTCCGAGCC TTGCTTCAAC 
ACCCTGCGCA CCAAGGAGCA ACTGGGCTAT ATCGTCTTCA GCGGGCCACG CCGAGCCAAT 
GGCATTCAGG GCTTACGGTT TATCATCCAG TCAGAAAAGC CACCTCACTA CCTAGAAAGC 
AGAGTGGAAG CTTTCTTAAT TACCATGGAA AAGTCCATAG AGGACATGAC AGAAGAGGCC 
TTCCAAAAAC ACATCCAAGC ATTAGCAATC CGTCGACTAG ACAAACCAAA GAAGCTATCT 
GCAGAGTGTG CTAAATATTG GGGGGAGATC ATCTCTCAGC AGTACAATTT TGACAGAGAT 
AATATAGAAG TTGCCTATTT GAAGACACTT ACCAAGGAAG ATATTATCAA ATTCTATAAG 
GAAATGTTGG CGGTAGATGC CCCGAGGAGA CACAAGGTAT CCGTCCATGT TCTTGCCAGA 
GAAATGGATT CTTGTCCTGT GGTTGGAGAG TTCCCATGTC AAAATGATAT AAATTTGTCA 
CAAGCACCAG CCTTGCCACA ACCTGAAGTG ATTCAGAACA TGACTGAGTT CAAGCGTGGT 
TTGCCACTGT TCCCCCTTGT GAAACCACAT ATTAACTTCA TGGCTGCAAA ACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IDE ( XM_015460599.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015316085.1 Bos taurus insulin degrading enzyme (IDE), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa230877
Clone ID Related Accession (Same CDS sequence)	XM_015460599.2
Accession Version	XM_015460599.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2937bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	insulin-degrading enzyme isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037353.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015460599.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATAATC CAGCAATCAA GAGAATAGGA CATCATATCA TCAAGTCTCA TGAAGACAAG 
CGAGAGTACC GCGGACTTGA GCTGGCCAAT GGGATCAAAG TACTTCTTGT CAGTGATCCA 
ACAACAGATA AATCATCAGC AGCACTTGAT GTACACATAG GTTCATTGTC AGATCCTCCA 
AATATTGCTG GCTTAAGTCA TTTTTGTGAG CATATGTTAT TTTTGGGAAC AAAGAAATAC 
CCTAAAGAAA ATGAATACAG CCAGTTTCTC AGTGAACATG CAGGAAGTTC AAACGCCTTC 
ACTAGTGGAG AGCATACCAA TTACTATTTT GATGTTTCTC ATGAACACCT AGAAGGTGCG 
CTAGACAGAT TTGCACAGTT TTTCCTGTGC CCCTTGTTTG ATGAGAGCTG CAAAGACAGA 
GAGGTGAATG CAGTTGATTC AGAACATGAG AAGAATGTGA TGAACGATGC CTGGAGACTC 
TTTCAGCTGG AAAAAGCTAC AGGGAATCCC AAACATCCCT TCAGTAAATT TGGAACAGGT 
AACAAATATA CTCTAGAGAC TAGACCGAAC CAAGAAGGCA TTGATGTAAG ACAAGAGCTC 
CTGAAATTCC ATTCTACTTA TTATTCATCG AACTTAATGG CTATTTGTGT TTTAGGTCGA 
GAATCTTTGG ATGATTTGAC TAATCTGGTG GTAAAGTTAT TTTCTGAAGT AGAGAACAAA 
AATGTCCCGT TGCCAGAATT TCCTGAACAT CCTTTCCAAG AAGAACATCT TAAACAACTT 
TACAAAATAG TACCCATTAA GGATATTAGG AATCTTTATG TGACATTTCC CATACCTGAC 
CTTCAGAAAT ACTACAAATC AAATCCCGGT CATTATCTCG GTCATCTCAT TGGGCATGAA 
GGTCCTGGAA GTCTGTTATC AGAACTTAAA TCAAAGGGCT GGGTAAATAC TCTTGTTGGT 
GGGCAAAAGG AAGGAGCCCG AGGTTTTATG TTTTTTATCA TTAATGTGGA CTTGACTGAG 
GAAGGATTAT TACATGTTGA GGATATAATT TTGCACATGT TTCAATACAT TCAGAAGTTA 
CGAGCAGAAG GACCTCAAGA ATGGGTTTTC CAAGAGTGCA AGGACTTGAA TGCTGTTGCT 
TTTAGGTTTA AAGATAAAGA GAGACCACGG GGTTATACCT CTAAGATTGC AGGAATATTA 
CATTATTATC CCCTAGAAGA AGTGCTCACA GCTGAATATT TACTGGAAGA ATTTAGACCA 
GATTTAATAG AGATGGTTCT CGATAAACTC AGACCAGAAA ATGTCCGGGT TGCAATAGTT 
TCCAAATCTT TTGAAGGAAA AACTGATCGC ACAGAAGAGT GGTACGGAAC CCAGTACAAA 
CAAGAAGCTA TACCAGACGA AGTCATTAAG AAATGGCAAA ATGCTGATCT GAATGGGAAA 
TTTAAACTTC CAATGAAGAA TGAATTTATT CCTACAAATT TTGAAATTTT ATCATTAGAA 
AAAGAAGCAA CGCCATACCC TTCTCTTATT AAGGATACAG CTATGAGCAA ACTCTGGTTC 
AAACAAGATG ATAAGTTTTT CTTGCCCAAG GCCTGTCTCA ACTTTGAATT TTTCAGCCCA 
TTTGCTTATG TGGACCCCTT GCACTGTAAC ATGGCCTATT TGTACCTTGA GCTCCTCAAA 
GACTCACTCA ACGAGTATGC ATATGCAGCA GAGCTAGCAG GCTTGAGCTA TGACCTCCAA 
AATACCATCT ATGGGATGTA TCTCTCGGTG AAAGGTTACA ATGACAAGCA GCCAATTTTG 
CTAAAGAAGA TCATTGAGAA AATGGCTACC TTTGAGATTG ATGAAAAAAG ATTTGAAATT 
ATCAAAGAGG CATATATGCG ATCTCTTAAC AATTTCCGAG CTGAACAGCC TCATCAACAC 
GCCATGTACT ACCTCCGCTT GCTGATGACT GAAGTGGCCT GGACTAAAGA TGAATTAAAA 
GAAGCCTTGG ATGATGTCAC CCTTCCTCGC CTTAAGGCCT TCATACCTCA ACTCCTGTCA 
CGGCTGCATA TTGAAGCCCT TCTCCATGGA AATATAACAA AGCAGGCTGC GTTAGGAATC 
ATGCAGATGG TTGAAGACAC CCTTATTGAA CATGCTCATA CGAAACCACT TCTTCCAAGT 
CAGCTCGTTC GGTACAGAGA AGTTCAGCTC CCGGACAGGG GATGGTTTGT GTATCAGCAG 
AGGAATGAAG TCCACAATAA CTGTGGCATT GAGATATACT ACCAAACCGA CATGCAGAGC 
ACGTCAGAGA ACATGTTTCT GGAGCTCTTC TGTCAGATCA TCTCCGAGCC TTGCTTCAAC 
ACCCTGCGCA CCAAGGAGCA ACTGGGCTAT ATCGTCTTCA GCGGGCCACG CCGAGCCAAT 
GGCATTCAGG GCTTACGGTT TATCATCCAG TCAGAAAAGC CACCTCACTA CCTAGAAAGC 
AGAGTGGAAG CTTTCTTAAT TACCATGGAA AAGTCCATAG AGGACATGAC AGAAGAGGCC 
TTCCAAAAAC ACATCCAAGC ATTAGCAATC CGTCGACTAG ACAAACCAAA GAAGCTATCT 
GCAGAGTGTG CTAAATATTG GGGGGAGATC ATCTCTCAGC AGTACAATTT TGACAGAGAT 
AATATAGAAG TTGCCTATTT GAAGACACTT ACCAAGGAAG ATATTATCAA ATTCTATAAG 
GAAATGTTGG CGGTAGATGC CCCGAGGAGA CACAAGGTAT CCGTCCATGT TCTTGCCAGA 
GAAATGGATT CTTGTCCTGT GGTTGGAGAG TTCCCATGTC AAAATGATAT AAATTTGTCA 
CAAGCACCAG CCTTGCCACA ACCTGAAGTG ATTCAGAACA TGACTGAGTT CAAGCGTGGT 
TTGCCACTGT TCCCCCTTGT GAAACCACAT ATTAACTTCA TGGCTGCAAA ACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015316085.1
CDS	365..3301
Translation	MNNPAIKRIGHHIIKSHEDKREYRGLELANGIKVLLVSDPTTDKSSAALDVHIGSLSDPPNIAGLSHFCEHMLFLGTKKYPKENEYSQFLSEHAGSSNAFTSGEHTNYYFDVSHEHLEGALDRFAQFFLCPLFDESCKDREVNAVDSEHEKNVMNDAWRLFQLEKATGNPKHPFSKFGTGNKYTLETRPNQEGIDVRQELLKFHSTYYSSNLMAICVLGRESLDDLTNLVVKLFSEVENKNVPLPEFPEHPFQEEHLKQLYKIVPIKDIRNLYVTFPIPDLQKYYKSNPGHYLGHLIGHEGPGSLLSELKSKGWVNTLVGGQKEGARGFMFFIINVDLTEEGLLHVEDIILHMFQYIQKLRAEGPQEWVFQECKDLNAVAFRFKDKERPRGYTSKIAGILHYYPLEEVLTAEYLLEEFRPDLIEMVLDKLRPENVRVAIVSKSFEGKTDRTEEWYGTQYKQEAIPDEVIKKWQNADLNGKFKLPMKNEFIPTNFEILSLEKEATPYPSLIKDTAMSKLWFKQDDKFFLPKACLNFEFFSPFAYVDPLHCNMAYLYLELLKDSLNEYAYAAELAGLSYDLQNTIYGMYLSVKGYNDKQPILLKKIIEKMATFEIDEKRFEIIKEAYMRSLNNFRAEQPHQHAMYYLRLLMTEVAWTKDELKEALDDVTLPRLKAFIPQLLSRLHIEALLHGNITKQAALGIMQMVEDTLIEHAHTKPLLPSQLVRYREVQLPDRGWFVYQQRNEVHNNCGIEIYYQTDMQSTSENMFLELFCQIISEPCFNTLRTKEQLGYIVFSGPRRANGIQGLRFIIQSEKPPHYLESRVEAFLITMEKSIEDMTEEAFQKHIQALAIRRLDKPKKLSAECAKYWGEIISQQYNFDRDNIEVAYLKTLTKEDIIKFYKEMLAVDAPRRHKVSVHVLAREMDSCPVVGEFPCQNDINLSQAPALPQPEVIQNMTEFKRGLPLFPLVKPHINFMAAKL

Target ORF information:

RefSeq Version	XM_015460599.2
Organism	Bos taurus(cattle)
Definition	Bos taurus insulin degrading enzyme (IDE), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015460599.2

ORF Insert Sequence:

ATGAATAATC CAGCAATCAA GAGAATAGGA CATCATATCA TCAAGTCTCA TGAAGACAAG 
CGAGAGTACC GCGGACTTGA GCTGGCCAAT GGGATCAAAG TACTTCTTGT CAGTGATCCA 
ACAACAGATA AATCATCAGC AGCACTTGAT GTACACATAG GTTCATTGTC AGATCCTCCA 
AATATTGCTG GCTTAAGTCA TTTTTGTGAG CATATGTTAT TTTTGGGAAC AAAGAAATAC 
CCTAAAGAAA ATGAATACAG CCAGTTTCTC AGTGAACATG CAGGAAGTTC AAACGCCTTC 
ACTAGTGGAG AGCATACCAA TTACTATTTT GATGTTTCTC ATGAACACCT AGAAGGTGCG 
CTAGACAGAT TTGCACAGTT TTTCCTGTGC CCCTTGTTTG ATGAGAGCTG CAAAGACAGA 
GAGGTGAATG CAGTTGATTC AGAACATGAG AAGAATGTGA TGAACGATGC CTGGAGACTC 
TTTCAGCTGG AAAAAGCTAC AGGGAATCCC AAACATCCCT TCAGTAAATT TGGAACAGGT 
AACAAATATA CTCTAGAGAC TAGACCGAAC CAAGAAGGCA TTGATGTAAG ACAAGAGCTC 
CTGAAATTCC ATTCTACTTA TTATTCATCG AACTTAATGG CTATTTGTGT TTTAGGTCGA 
GAATCTTTGG ATGATTTGAC TAATCTGGTG GTAAAGTTAT TTTCTGAAGT AGAGAACAAA 
AATGTCCCGT TGCCAGAATT TCCTGAACAT CCTTTCCAAG AAGAACATCT TAAACAACTT 
TACAAAATAG TACCCATTAA GGATATTAGG AATCTTTATG TGACATTTCC CATACCTGAC 
CTTCAGAAAT ACTACAAATC AAATCCCGGT CATTATCTCG GTCATCTCAT TGGGCATGAA 
GGTCCTGGAA GTCTGTTATC AGAACTTAAA TCAAAGGGCT GGGTAAATAC TCTTGTTGGT 
GGGCAAAAGG AAGGAGCCCG AGGTTTTATG TTTTTTATCA TTAATGTGGA CTTGACTGAG 
GAAGGATTAT TACATGTTGA GGATATAATT TTGCACATGT TTCAATACAT TCAGAAGTTA 
CGAGCAGAAG GACCTCAAGA ATGGGTTTTC CAAGAGTGCA AGGACTTGAA TGCTGTTGCT 
TTTAGGTTTA AAGATAAAGA GAGACCACGG GGTTATACCT CTAAGATTGC AGGAATATTA 
CATTATTATC CCCTAGAAGA AGTGCTCACA GCTGAATATT TACTGGAAGA ATTTAGACCA 
GATTTAATAG AGATGGTTCT CGATAAACTC AGACCAGAAA ATGTCCGGGT TGCAATAGTT 
TCCAAATCTT TTGAAGGAAA AACTGATCGC ACAGAAGAGT GGTACGGAAC CCAGTACAAA 
CAAGAAGCTA TACCAGACGA AGTCATTAAG AAATGGCAAA ATGCTGATCT GAATGGGAAA 
TTTAAACTTC CAATGAAGAA TGAATTTATT CCTACAAATT TTGAAATTTT ATCATTAGAA 
AAAGAAGCAA CGCCATACCC TTCTCTTATT AAGGATACAG CTATGAGCAA ACTCTGGTTC 
AAACAAGATG ATAAGTTTTT CTTGCCCAAG GCCTGTCTCA ACTTTGAATT TTTCAGCCCA 
TTTGCTTATG TGGACCCCTT GCACTGTAAC ATGGCCTATT TGTACCTTGA GCTCCTCAAA 
GACTCACTCA ACGAGTATGC ATATGCAGCA GAGCTAGCAG GCTTGAGCTA TGACCTCCAA 
AATACCATCT ATGGGATGTA TCTCTCGGTG AAAGGTTACA ATGACAAGCA GCCAATTTTG 
CTAAAGAAGA TCATTGAGAA AATGGCTACC TTTGAGATTG ATGAAAAAAG ATTTGAAATT 
ATCAAAGAGG CATATATGCG ATCTCTTAAC AATTTCCGAG CTGAACAGCC TCATCAACAC 
GCCATGTACT ACCTCCGCTT GCTGATGACT GAAGTGGCCT GGACTAAAGA TGAATTAAAA 
GAAGCCTTGG ATGATGTCAC CCTTCCTCGC CTTAAGGCCT TCATACCTCA ACTCCTGTCA 
CGGCTGCATA TTGAAGCCCT TCTCCATGGA AATATAACAA AGCAGGCTGC GTTAGGAATC 
ATGCAGATGG TTGAAGACAC CCTTATTGAA CATGCTCATA CGAAACCACT TCTTCCAAGT 
CAGCTCGTTC GGTACAGAGA AGTTCAGCTC CCGGACAGGG GATGGTTTGT GTATCAGCAG 
AGGAATGAAG TCCACAATAA CTGTGGCATT GAGATATACT ACCAAACCGA CATGCAGAGC 
ACGTCAGAGA ACATGTTTCT GGAGCTCTTC TGTCAGATCA TCTCCGAGCC TTGCTTCAAC 
ACCCTGCGCA CCAAGGAGCA ACTGGGCTAT ATCGTCTTCA GCGGGCCACG CCGAGCCAAT 
GGCATTCAGG GCTTACGGTT TATCATCCAG TCAGAAAAGC CACCTCACTA CCTAGAAAGC 
AGAGTGGAAG CTTTCTTAAT TACCATGGAA AAGTCCATAG AGGACATGAC AGAAGAGGCC 
TTCCAAAAAC ACATCCAAGC ATTAGCAATC CGTCGACTAG ACAAACCAAA GAAGCTATCT 
GCAGAGTGTG CTAAATATTG GGGGGAGATC ATCTCTCAGC AGTACAATTT TGACAGAGAT 
AATATAGAAG TTGCCTATTT GAAGACACTT ACCAAGGAAG ATATTATCAA ATTCTATAAG 
GAAATGTTGG CGGTAGATGC CCCGAGGAGA CACAAGGTAT CCGTCCATGT TCTTGCCAGA 
GAAATGGATT CTTGTCCTGT GGTTGGAGAG TTCCCATGTC AAAATGATAT AAATTTGTCA 
CAAGCACCAG CCTTGCCACA ACCTGAAGTG ATTCAGAACA TGACTGAGTT CAAGCGTGGT 
TTGCCACTGT TCCCCCTTGT GAAACCACAT ATTAACTTCA TGGCTGCAAA ACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IDE ( XM_010819775.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010818077.1 Bos taurus insulin degrading enzyme (IDE), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa230876
Clone ID Related Accession (Same CDS sequence)	XM_010819775.3
Accession Version	XM_010819775.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3060bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	insulin-degrading enzyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037353.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010819775.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGTACC GGCTAGCGTG GCTGCTGCAT TCCGCGCTGC CCAGCACCTT CCGCTCGGTC 
CTCGGCGCCC GCCTGCCGCC CTCGGAGCGC CTCTGTGGTT TCCAAAAAAA GACTTACAGC 
AAAATGAATA ATCCAGCAAT CAAGAGAATA GGACATCATA TCATCAAGTC TCATGAAGAC 
AAGCGAGAGT ACCGCGGACT TGAGCTGGCC AATGGGATCA AAGTACTTCT TGTCAGTGAT 
CCAACAACAG ATAAATCATC AGCAGCACTT GATGTACACA TAGGTTCATT GTCAGATCCT 
CCAAATATTG CTGGCTTAAG TCATTTTTGT GAGCATATGT TATTTTTGGG AACAAAGAAA 
TACCCTAAAG AAAATGAATA CAGCCAGTTT CTCAGTGAAC ATGCAGGAAG TTCAAACGCC 
TTCACTAGTG GAGAGCATAC CAATTACTAT TTTGATGTTT CTCATGAACA CCTAGAAGGT 
GCGCTAGACA GATTTGCACA GTTTTTCCTG TGCCCCTTGT TTGATGAGAG CTGCAAAGAC 
AGAGAGGTGA ATGCAGTTGA TTCAGAACAT GAGAAGAATG TGATGAACGA TGCCTGGAGA 
CTCTTTCAGC TGGAAAAAGC TACAGGGAAT CCCAAACATC CCTTCAGTAA ATTTGGAACA 
GGTAACAAAT ATACTCTAGA GACTAGACCG AACCAAGAAG GCATTGATGT AAGACAAGAG 
CTCCTGAAAT TCCATTCTAC TTATTATTCA TCGAACTTAA TGGCTATTTG TGTTTTAGGT 
CGAGAATCTT TGGATGATTT GACTAATCTG GTGGTAAAGT TATTTTCTGA AGTAGAGAAC 
AAAAATGTCC CGTTGCCAGA ATTTCCTGAA CATCCTTTCC AAGAAGAACA TCTTAAACAA 
CTTTACAAAA TAGTACCCAT TAAGGATATT AGGAATCTTT ATGTGACATT TCCCATACCT 
GACCTTCAGA AATACTACAA ATCAAATCCC GGTCATTATC TCGGTCATCT CATTGGGCAT 
GAAGGTCCTG GAAGTCTGTT ATCAGAACTT AAATCAAAGG GCTGGGTAAA TACTCTTGTT 
GGTGGGCAAA AGGAAGGAGC CCGAGGTTTT ATGTTTTTTA TCATTAATGT GGACTTGACT 
GAGGAAGGAT TATTACATGT TGAGGATATA ATTTTGCACA TGTTTCAATA CATTCAGAAG 
TTACGAGCAG AAGGACCTCA AGAATGGGTT TTCCAAGAGT GCAAGGACTT GAATGCTGTT 
GCTTTTAGGT TTAAAGATAA AGAGAGACCA CGGGGTTATA CCTCTAAGAT TGCAGGAATA 
TTACATTATT ATCCCCTAGA AGAAGTGCTC ACAGCTGAAT ATTTACTGGA AGAATTTAGA 
CCAGATTTAA TAGAGATGGT TCTCGATAAA CTCAGACCAG AAAATGTCCG GGTTGCAATA 
GTTTCCAAAT CTTTTGAAGG AAAAACTGAT CGCACAGAAG AGTGGTACGG AACCCAGTAC 
AAACAAGAAG CTATACCAGA CGAAGTCATT AAGAAATGGC AAAATGCTGA TCTGAATGGG 
AAATTTAAAC TTCCAATGAA GAATGAATTT ATTCCTACAA ATTTTGAAAT TTTATCATTA 
GAAAAAGAAG CAACGCCATA CCCTTCTCTT ATTAAGGATA CAGCTATGAG CAAACTCTGG 
TTCAAACAAG ATGATAAGTT TTTCTTGCCC AAGGCCTGTC TCAACTTTGA ATTTTTCAGT 
CGCTACATTT ATGCTGATCC TCTCCATTGC AACATGACAT ACCTGTTTAT CAGGTTATTG 
AAGGATGATT TAAAAGAGTA TACATATGCA GCACGCCTCT CAGGTTTGAG CTATGGCATT 
GCATCAGGAA TGAATGCAAT ACTTCTCTCG GTGAAAGGTT ACAATGACAA GCAGCCAATT 
TTGCTAAAGA AGATCATTGA GAAAATGGCT ACCTTTGAGA TTGATGAAAA AAGATTTGAA 
ATTATCAAAG AGGCATATAT GCGATCTCTT AACAATTTCC GAGCTGAACA GCCTCATCAA 
CACGCCATGT ACTACCTCCG CTTGCTGATG ACTGAAGTGG CCTGGACTAA AGATGAATTA 
AAAGAAGCCT TGGATGATGT CACCCTTCCT CGCCTTAAGG CCTTCATACC TCAACTCCTG 
TCACGGCTGC ATATTGAAGC CCTTCTCCAT GGAAATATAA CAAAGCAGGC TGCGTTAGGA 
ATCATGCAGA TGGTTGAAGA CACCCTTATT GAACATGCTC ATACGAAACC ACTTCTTCCA 
AGTCAGCTCG TTCGGTACAG AGAAGTTCAG CTCCCGGACA GGGGATGGTT TGTGTATCAG 
CAGAGGAATG AAGTCCACAA TAACTGTGGC ATTGAGATAT ACTACCAAAC CGACATGCAG 
AGCACGTCAG AGAACATGTT TCTGGAGCTC TTCTGTCAGA TCATCTCCGA GCCTTGCTTC 
AACACCCTGC GCACCAAGGA GCAACTGGGC TATATCGTCT TCAGCGGGCC ACGCCGAGCC 
AATGGCATTC AGGGCTTACG GTTTATCATC CAGTCAGAAA AGCCACCTCA CTACCTAGAA 
AGCAGAGTGG AAGCTTTCTT AATTACCATG GAAAAGTCCA TAGAGGACAT GACAGAAGAG 
GCCTTCCAAA AACACATCCA AGCATTAGCA ATCCGTCGAC TAGACAAACC AAAGAAGCTA 
TCTGCAGAGT GTGCTAAATA TTGGGGGGAG ATCATCTCTC AGCAGTACAA TTTTGACAGA 
GATAATATAG AAGTTGCCTA TTTGAAGACA CTTACCAAGG AAGATATTAT CAAATTCTAT 
AAGGAAATGT TGGCGGTAGA TGCCCCGAGG AGACACAAGG TATCCGTCCA TGTTCTTGCC 
AGAGAAATGG ATTCTTGTCC TGTGGTTGGA GAGTTCCCAT GTCAAAATGA TATAAATTTG 
TCACAAGCAC CAGCCTTGCC ACAACCTGAA GTGATTCAGA ACATGACTGA GTTCAAGCGT 
GGTTTGCCAC TGTTCCCCCT TGTGAAACCA CATATTAACT TCATGGCTGC AAAACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010818077.1
CDS	64..3123
Translation	MRYRLAWLLHSALPSTFRSVLGARLPPSERLCGFQKKTYSKMNNPAIKRIGHHIIKSHEDKREYRGLELANGIKVLLVSDPTTDKSSAALDVHIGSLSDPPNIAGLSHFCEHMLFLGTKKYPKENEYSQFLSEHAGSSNAFTSGEHTNYYFDVSHEHLEGALDRFAQFFLCPLFDESCKDREVNAVDSEHEKNVMNDAWRLFQLEKATGNPKHPFSKFGTGNKYTLETRPNQEGIDVRQELLKFHSTYYSSNLMAICVLGRESLDDLTNLVVKLFSEVENKNVPLPEFPEHPFQEEHLKQLYKIVPIKDIRNLYVTFPIPDLQKYYKSNPGHYLGHLIGHEGPGSLLSELKSKGWVNTLVGGQKEGARGFMFFIINVDLTEEGLLHVEDIILHMFQYIQKLRAEGPQEWVFQECKDLNAVAFRFKDKERPRGYTSKIAGILHYYPLEEVLTAEYLLEEFRPDLIEMVLDKLRPENVRVAIVSKSFEGKTDRTEEWYGTQYKQEAIPDEVIKKWQNADLNGKFKLPMKNEFIPTNFEILSLEKEATPYPSLIKDTAMSKLWFKQDDKFFLPKACLNFEFFSRYIYADPLHCNMTYLFIRLLKDDLKEYTYAARLSGLSYGIASGMNAILLSVKGYNDKQPILLKKIIEKMATFEIDEKRFEIIKEAYMRSLNNFRAEQPHQHAMYYLRLLMTEVAWTKDELKEALDDVTLPRLKAFIPQLLSRLHIEALLHGNITKQAALGIMQMVEDTLIEHAHTKPLLPSQLVRYREVQLPDRGWFVYQQRNEVHNNCGIEIYYQTDMQSTSENMFLELFCQIISEPCFNTLRTKEQLGYIVFSGPRRANGIQGLRFIIQSEKPPHYLESRVEAFLITMEKSIEDMTEEAFQKHIQALAIRRLDKPKKLSAECAKYWGEIISQQYNFDRDNIEVAYLKTLTKEDIIKFYKEMLAVDAPRRHKVSVHVLAREMDSCPVVGEFPCQNDINLSQAPALPQPEVIQNMTEFKRGLPLFPLVKPHINFMAAKL

Target ORF information:

RefSeq Version	XM_010819775.3
Organism	Bos taurus(cattle)
Definition	Bos taurus insulin degrading enzyme (IDE), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010819775.3

ORF Insert Sequence:

ATGCGGTACC GGCTAGCGTG GCTGCTGCAT TCCGCGCTGC CCAGCACCTT CCGCTCGGTC 
CTCGGCGCCC GCCTGCCGCC CTCGGAGCGC CTCTGTGGTT TCCAAAAAAA GACTTACAGC 
AAAATGAATA ATCCAGCAAT CAAGAGAATA GGACATCATA TCATCAAGTC TCATGAAGAC 
AAGCGAGAGT ACCGCGGACT TGAGCTGGCC AATGGGATCA AAGTACTTCT TGTCAGTGAT 
CCAACAACAG ATAAATCATC AGCAGCACTT GATGTACACA TAGGTTCATT GTCAGATCCT 
CCAAATATTG CTGGCTTAAG TCATTTTTGT GAGCATATGT TATTTTTGGG AACAAAGAAA 
TACCCTAAAG AAAATGAATA CAGCCAGTTT CTCAGTGAAC ATGCAGGAAG TTCAAACGCC 
TTCACTAGTG GAGAGCATAC CAATTACTAT TTTGATGTTT CTCATGAACA CCTAGAAGGT 
GCGCTAGACA GATTTGCACA GTTTTTCCTG TGCCCCTTGT TTGATGAGAG CTGCAAAGAC 
AGAGAGGTGA ATGCAGTTGA TTCAGAACAT GAGAAGAATG TGATGAACGA TGCCTGGAGA 
CTCTTTCAGC TGGAAAAAGC TACAGGGAAT CCCAAACATC CCTTCAGTAA ATTTGGAACA 
GGTAACAAAT ATACTCTAGA GACTAGACCG AACCAAGAAG GCATTGATGT AAGACAAGAG 
CTCCTGAAAT TCCATTCTAC TTATTATTCA TCGAACTTAA TGGCTATTTG TGTTTTAGGT 
CGAGAATCTT TGGATGATTT GACTAATCTG GTGGTAAAGT TATTTTCTGA AGTAGAGAAC 
AAAAATGTCC CGTTGCCAGA ATTTCCTGAA CATCCTTTCC AAGAAGAACA TCTTAAACAA 
CTTTACAAAA TAGTACCCAT TAAGGATATT AGGAATCTTT ATGTGACATT TCCCATACCT 
GACCTTCAGA AATACTACAA ATCAAATCCC GGTCATTATC TCGGTCATCT CATTGGGCAT 
GAAGGTCCTG GAAGTCTGTT ATCAGAACTT AAATCAAAGG GCTGGGTAAA TACTCTTGTT 
GGTGGGCAAA AGGAAGGAGC CCGAGGTTTT ATGTTTTTTA TCATTAATGT GGACTTGACT 
GAGGAAGGAT TATTACATGT TGAGGATATA ATTTTGCACA TGTTTCAATA CATTCAGAAG 
TTACGAGCAG AAGGACCTCA AGAATGGGTT TTCCAAGAGT GCAAGGACTT GAATGCTGTT 
GCTTTTAGGT TTAAAGATAA AGAGAGACCA CGGGGTTATA CCTCTAAGAT TGCAGGAATA 
TTACATTATT ATCCCCTAGA AGAAGTGCTC ACAGCTGAAT ATTTACTGGA AGAATTTAGA 
CCAGATTTAA TAGAGATGGT TCTCGATAAA CTCAGACCAG AAAATGTCCG GGTTGCAATA 
GTTTCCAAAT CTTTTGAAGG AAAAACTGAT CGCACAGAAG AGTGGTACGG AACCCAGTAC 
AAACAAGAAG CTATACCAGA CGAAGTCATT AAGAAATGGC AAAATGCTGA TCTGAATGGG 
AAATTTAAAC TTCCAATGAA GAATGAATTT ATTCCTACAA ATTTTGAAAT TTTATCATTA 
GAAAAAGAAG CAACGCCATA CCCTTCTCTT ATTAAGGATA CAGCTATGAG CAAACTCTGG 
TTCAAACAAG ATGATAAGTT TTTCTTGCCC AAGGCCTGTC TCAACTTTGA ATTTTTCAGT 
CGCTACATTT ATGCTGATCC TCTCCATTGC AACATGACAT ACCTGTTTAT CAGGTTATTG 
AAGGATGATT TAAAAGAGTA TACATATGCA GCACGCCTCT CAGGTTTGAG CTATGGCATT 
GCATCAGGAA TGAATGCAAT ACTTCTCTCG GTGAAAGGTT ACAATGACAA GCAGCCAATT 
TTGCTAAAGA AGATCATTGA GAAAATGGCT ACCTTTGAGA TTGATGAAAA AAGATTTGAA 
ATTATCAAAG AGGCATATAT GCGATCTCTT AACAATTTCC GAGCTGAACA GCCTCATCAA 
CACGCCATGT ACTACCTCCG CTTGCTGATG ACTGAAGTGG CCTGGACTAA AGATGAATTA 
AAAGAAGCCT TGGATGATGT CACCCTTCCT CGCCTTAAGG CCTTCATACC TCAACTCCTG 
TCACGGCTGC ATATTGAAGC CCTTCTCCAT GGAAATATAA CAAAGCAGGC TGCGTTAGGA 
ATCATGCAGA TGGTTGAAGA CACCCTTATT GAACATGCTC ATACGAAACC ACTTCTTCCA 
AGTCAGCTCG TTCGGTACAG AGAAGTTCAG CTCCCGGACA GGGGATGGTT TGTGTATCAG 
CAGAGGAATG AAGTCCACAA TAACTGTGGC ATTGAGATAT ACTACCAAAC CGACATGCAG 
AGCACGTCAG AGAACATGTT TCTGGAGCTC TTCTGTCAGA TCATCTCCGA GCCTTGCTTC 
AACACCCTGC GCACCAAGGA GCAACTGGGC TATATCGTCT TCAGCGGGCC ACGCCGAGCC 
AATGGCATTC AGGGCTTACG GTTTATCATC CAGTCAGAAA AGCCACCTCA CTACCTAGAA 
AGCAGAGTGG AAGCTTTCTT AATTACCATG GAAAAGTCCA TAGAGGACAT GACAGAAGAG 
GCCTTCCAAA AACACATCCA AGCATTAGCA ATCCGTCGAC TAGACAAACC AAAGAAGCTA 
TCTGCAGAGT GTGCTAAATA TTGGGGGGAG ATCATCTCTC AGCAGTACAA TTTTGACAGA 
GATAATATAG AAGTTGCCTA TTTGAAGACA CTTACCAAGG AAGATATTAT CAAATTCTAT 
AAGGAAATGT TGGCGGTAGA TGCCCCGAGG AGACACAAGG TATCCGTCCA TGTTCTTGCC 
AGAGAAATGG ATTCTTGTCC TGTGGTTGGA GAGTTCCCAT GTCAAAATGA TATAAATTTG 
TCACAAGCAC CAGCCTTGCC ACAACCTGAA GTGATTCAGA ACATGACTGA GTTCAAGCGT 
GGTTTGCCAC TGTTCCCCCT TGTGAAACCA CATATTAACT TCATGGCTGC AAAACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IDE ( NM_001075849.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001069317.1 Bos taurus insulin degrading enzyme (IDE), mRNA.

Price & Availability↑

CloneID	OBa42699
Clone ID Related Accession (Same CDS sequence)	NM_001075849.1
Accession Version	NM_001075849.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3060bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Bos taurus(cattle)
Product	insulin-degrading enzyme precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC114105.1. On Oct 4, 2006 this sequence version replaced XM_602055.2. ##Evidence-Data-START## Transcript exon combination :: BC114105.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGCGGTACC GGCTAGCGTG GCTGCTGCAT TCCGCGCTGC CCAGCACCTT CCGCTCGGTC 
CTCGGCGCCC GCCTGCCGCC CTCGGAGCGC CTCTGTGGTT TCCAAAAAAA GACTTACAGC 
AAAATGAATA ATCCAGCAAT CAAGAGAATA GGACATCATA TCATCAAGTC TCATGAAGAC 
AAGCGAGAAT ACCGCGGACT TGAGCTGGCC AATGGGATCA AAGTACTTCT TGTCAGTGAT 
CCAACAACAG ATAAATCATC AGCAGCACTT GATGTACACA TAGGTTCATT GTCAGATCCT 
CCAAATATTG CTGGCTTAAG TCATTTTTGT GAGCATATGT TATTTTTGGG AACAAAGAAA 
TACCCTAAAG AAAATGAATA CAGCCAGTTT CTCAGTGAAC ATGCAGGAAG TTCAAACGCC 
TTCACTAGTG GAGAGCATAC CAATTACTAT TTTGATGTTT CTCATGAACA CCTAGAAGGT 
GCGCTAGACA GATTTGCACA GTTTTTCCTG TGCCCCTTGT TTGATGAGAG CTGCAAAGAC 
AGAGAGGTGA ATGCAGTTGA TTCAGAACAT GAGAAGAATG TGATGAATGA TGCCTGGAGA 
CTCTTTCAGC TGGAAAAAGC TACAGGGAAT CCCAAACATC CCTTCAGTAA ATTTGGAACA 
GGTAACAAAT ATACTCTAGA GACTAGACCG AACCAAGAAG GCATTGATGT AAGACAAGAG 
CTCCTGAAAT TCCATTCTAC TTATTATTCA TCGAACTTAA TGGCTATTTG TGTTTTAGGT 
CGAGAATCTT TGGATGATTT GACTAATCTG GTGGTAAAGT TATTTTCTGA AGTAGAGAAC 
AAAAATGTCC CGTTGCCAGA ATTTCCTGAA CATCCTTTCC AAGAAGAACA TCTTAAACAA 
CTTTACAAAA TAGTACCCAT TAAGGATATT AGGAATCTTT ATGTGACATT TCCCATACCT 
GACCTTCAGA AATACTACAA ATCAAATCCT GGTCATTATC TCGGTCATCT CATTGGGCAT 
GAAGGTCCTG GAAGTCTGTT ATCAGAACTT AAATCAAAGG GCTGGGTAAA TACTCTTGTT 
GGTGGGCAAA AGGAAGGAGC CCGAGGTTTT ATGTTTTTTA TCATTAATGT GGACTTGACT 
GAGGAAGGAT TATTACATGT TGAGGATATA ATTTTGCACA TGTTTCAATA CATTCAGAAG 
TTACGAGCAG AAGGACCTCA AGAATGGGTT TTCCAAGAGT GCAAGGACTT GAATGCTGTT 
GCTTTTAGGT TTAAAGATAA AGAGAGACCA CGGGGTTATA CCTCTAAGAT TGCAGGAATA 
TTACATTATT ATCCCCTAGA AGAAGTGCTC ACAGCTGAAT ATTTACTGGA AGAATTTAGA 
CCAGATTTAA TAGAGATGGT TCTCGATAAA CTCAGACCAG AAAATGTCCG GGTTGCAATA 
GTTTCCAAAT CTTTTGAAGG AAAAACTGAT CGCACAGAAG AGTGGTACGG AACCCAGTAC 
AAACAAGAAG CTATACCAGA CGAAGTCATT AAGAAATGGC AAAATGCTGA TCTGAATGGG 
AAATTTAAAC TTCCAATGAA GAATGAATTT ATTCCTACAA ATTTTGAAAT TTTATCATTA 
GAAAAAGAAG CAACGCCATA CCCTTCTCTT ATTAAGGATA CAGCTATGAG CAAACTCTGG 
TTCAAACAAG ATGATAAGTT TTTCTTGCCC AAGGCCTGTC TCAACTTTGA ATTTTTCAGC 
CCATTTGCTT ATGTGGACCC CTTGCACTGT AACATGGCCT ATTTGTACCT TGAGCTCCTC 
AAAGACTCAC TCAACGAGTA TGCATATGCA GCAGAGCTAG CAGGCTTGAG CTATGACCTC 
CAAAATACCA TCTATGGGAT GTATCTCTCG GTGAAAGGTT ACAATGACAA GCAGCCAATT 
TTGCTAAAGA AGATCATTGA GAAAATGGCT ACCTTTGAGA TTGATGAAAA AAGATTTGAA 
ATTATCAAAG AGGCATATAT GCGATCTCTT AACAATTTCC GAGCTGAACA GCCTCATCAA 
CACGCCATGT ACTACCTCCG CTTGCTGATG ACTGAAGTGG CCTGGACTAA AGATGAATTA 
AAAGAAGCCT TGGATGATGT CACCCTTCCT CGCCTTAAGG CCTTCATACC TCAACTCCTG 
TCACGGCTGC ATATTGAAGC CCTTCTCCAT GGAAATATAA CAAAGCAGGC TGCGTTAGGA 
ATCATGCAGA TGGTTGAAGA CACCCTTATT GAACATGCTC ATACGAAACC ACTTCTTCCA 
AGTCAGCTCG TTCGGTACAG AGAAGTTCAG CTCCCGGACA GGGGATGGTT TGTGTATCAG 
CAGAGGAATG AAGTCCACAA TAACTGTGGC ATTGAGATAT ACTACCAAAC CGACATGCAG 
AGCACGTCAG AGAACATGTT TCTGGAGCTC TTCTGTCAGA TCATCTCCGA GCCTTGCTTC 
AACACCCTGC GCACCAAGGA GCAACTGGGC TATATCGTCT TCAGCGGGCC ACGCCGAGCC 
AATGGCATTC AGGGCTTACG GTTTATCATC CAGTCAGAAA AGCCACCTCA CTACCTAGAA 
AGCAGAGTGG AAGCTTTCTT AATTACCATG GAAAAGTCCA TAGAGGACAT GACAGAAGAG 
GCCTTCCAAA AACACATCCA AGCATTAGCA ATCCGTCGAC TAGACAAACC AAAGAAGCTA 
TCTGCAGAGT GTGCTAAATA TTGGGGGGAG ATCATCTCTC AGCAGTACAA TTTTGACAGA 
GATAATATAG AAGTTGCCTA TTTGAAGACA CTTACCAAGG AAGATATTAT CAAATTCTAT 
AAGGAAATGT TGGCGGTAGA TGCCCCGAGG AGACACAAGG TATCCGTCCA TGTTCTTGCC 
AGAGAAATGG ATTCTTGTCC TGTGGTTGGA GAGTTCCCAT GTCAAAATGA TATAAATTTG 
TCACAAGCAC CAGCCTTGCC ACAACCTGAA GTGATTCAGA ACATGACTGA GTTCAAGCGT 
GGTTTGCCAC TGTTCCCCCT TGTGAAACCA CATATTAACT TCATGGCTGC AAAACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001069317.1
CDS	18..3077
Translation	MRYRLAWLLHSALPSTFRSVLGARLPPSERLCGFQKKTYSKMNNPAIKRIGHHIIKSHEDKREYRGLELANGIKVLLVSDPTTDKSSAALDVHIGSLSDPPNIAGLSHFCEHMLFLGTKKYPKENEYSQFLSEHAGSSNAFTSGEHTNYYFDVSHEHLEGALDRFAQFFLCPLFDESCKDREVNAVDSEHEKNVMNDAWRLFQLEKATGNPKHPFSKFGTGNKYTLETRPNQEGIDVRQELLKFHSTYYSSNLMAICVLGRESLDDLTNLVVKLFSEVENKNVPLPEFPEHPFQEEHLKQLYKIVPIKDIRNLYVTFPIPDLQKYYKSNPGHYLGHLIGHEGPGSLLSELKSKGWVNTLVGGQKEGARGFMFFIINVDLTEEGLLHVEDIILHMFQYIQKLRAEGPQEWVFQECKDLNAVAFRFKDKERPRGYTSKIAGILHYYPLEEVLTAEYLLEEFRPDLIEMVLDKLRPENVRVAIVSKSFEGKTDRTEEWYGTQYKQEAIPDEVIKKWQNADLNGKFKLPMKNEFIPTNFEILSLEKEATPYPSLIKDTAMSKLWFKQDDKFFLPKACLNFEFFSPFAYVDPLHCNMAYLYLELLKDSLNEYAYAAELAGLSYDLQNTIYGMYLSVKGYNDKQPILLKKIIEKMATFEIDEKRFEIIKEAYMRSLNNFRAEQPHQHAMYYLRLLMTEVAWTKDELKEALDDVTLPRLKAFIPQLLSRLHIEALLHGNITKQAALGIMQMVEDTLIEHAHTKPLLPSQLVRYREVQLPDRGWFVYQQRNEVHNNCGIEIYYQTDMQSTSENMFLELFCQIISEPCFNTLRTKEQLGYIVFSGPRRANGIQGLRFIIQSEKPPHYLESRVEAFLITMEKSIEDMTEEAFQKHIQALAIRRLDKPKKLSAECAKYWGEIISQQYNFDRDNIEVAYLKTLTKEDIIKFYKEMLAVDAPRRHKVSVHVLAREMDSCPVVGEFPCQNDINLSQAPALPQPEVIQNMTEFKRGLPLFPLVKPHINFMAAKL

Target ORF information:

RefSeq Version	NM_001075849.1
Organism	Bos taurus(cattle)
Definition	Bos taurus insulin degrading enzyme (IDE), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001075849.1

ORF Insert Sequence:

ATGCGGTACC GGCTAGCGTG GCTGCTGCAT TCCGCGCTGC CCAGCACCTT CCGCTCGGTC 
CTCGGCGCCC GCCTGCCGCC CTCGGAGCGC CTCTGTGGTT TCCAAAAAAA GACTTACAGC 
AAAATGAATA ATCCAGCAAT CAAGAGAATA GGACATCATA TCATCAAGTC TCATGAAGAC 
AAGCGAGAAT ACCGCGGACT TGAGCTGGCC AATGGGATCA AAGTACTTCT TGTCAGTGAT 
CCAACAACAG ATAAATCATC AGCAGCACTT GATGTACACA TAGGTTCATT GTCAGATCCT 
CCAAATATTG CTGGCTTAAG TCATTTTTGT GAGCATATGT TATTTTTGGG AACAAAGAAA 
TACCCTAAAG AAAATGAATA CAGCCAGTTT CTCAGTGAAC ATGCAGGAAG TTCAAACGCC 
TTCACTAGTG GAGAGCATAC CAATTACTAT TTTGATGTTT CTCATGAACA CCTAGAAGGT 
GCGCTAGACA GATTTGCACA GTTTTTCCTG TGCCCCTTGT TTGATGAGAG CTGCAAAGAC 
AGAGAGGTGA ATGCAGTTGA TTCAGAACAT GAGAAGAATG TGATGAATGA TGCCTGGAGA 
CTCTTTCAGC TGGAAAAAGC TACAGGGAAT CCCAAACATC CCTTCAGTAA ATTTGGAACA 
GGTAACAAAT ATACTCTAGA GACTAGACCG AACCAAGAAG GCATTGATGT AAGACAAGAG 
CTCCTGAAAT TCCATTCTAC TTATTATTCA TCGAACTTAA TGGCTATTTG TGTTTTAGGT 
CGAGAATCTT TGGATGATTT GACTAATCTG GTGGTAAAGT TATTTTCTGA AGTAGAGAAC 
AAAAATGTCC CGTTGCCAGA ATTTCCTGAA CATCCTTTCC AAGAAGAACA TCTTAAACAA 
CTTTACAAAA TAGTACCCAT TAAGGATATT AGGAATCTTT ATGTGACATT TCCCATACCT 
GACCTTCAGA AATACTACAA ATCAAATCCT GGTCATTATC TCGGTCATCT CATTGGGCAT 
GAAGGTCCTG GAAGTCTGTT ATCAGAACTT AAATCAAAGG GCTGGGTAAA TACTCTTGTT 
GGTGGGCAAA AGGAAGGAGC CCGAGGTTTT ATGTTTTTTA TCATTAATGT GGACTTGACT 
GAGGAAGGAT TATTACATGT TGAGGATATA ATTTTGCACA TGTTTCAATA CATTCAGAAG 
TTACGAGCAG AAGGACCTCA AGAATGGGTT TTCCAAGAGT GCAAGGACTT GAATGCTGTT 
GCTTTTAGGT TTAAAGATAA AGAGAGACCA CGGGGTTATA CCTCTAAGAT TGCAGGAATA 
TTACATTATT ATCCCCTAGA AGAAGTGCTC ACAGCTGAAT ATTTACTGGA AGAATTTAGA 
CCAGATTTAA TAGAGATGGT TCTCGATAAA CTCAGACCAG AAAATGTCCG GGTTGCAATA 
GTTTCCAAAT CTTTTGAAGG AAAAACTGAT CGCACAGAAG AGTGGTACGG AACCCAGTAC 
AAACAAGAAG CTATACCAGA CGAAGTCATT AAGAAATGGC AAAATGCTGA TCTGAATGGG 
AAATTTAAAC TTCCAATGAA GAATGAATTT ATTCCTACAA ATTTTGAAAT TTTATCATTA 
GAAAAAGAAG CAACGCCATA CCCTTCTCTT ATTAAGGATA CAGCTATGAG CAAACTCTGG 
TTCAAACAAG ATGATAAGTT TTTCTTGCCC AAGGCCTGTC TCAACTTTGA ATTTTTCAGC 
CCATTTGCTT ATGTGGACCC CTTGCACTGT AACATGGCCT ATTTGTACCT TGAGCTCCTC 
AAAGACTCAC TCAACGAGTA TGCATATGCA GCAGAGCTAG CAGGCTTGAG CTATGACCTC 
CAAAATACCA TCTATGGGAT GTATCTCTCG GTGAAAGGTT ACAATGACAA GCAGCCAATT 
TTGCTAAAGA AGATCATTGA GAAAATGGCT ACCTTTGAGA TTGATGAAAA AAGATTTGAA 
ATTATCAAAG AGGCATATAT GCGATCTCTT AACAATTTCC GAGCTGAACA GCCTCATCAA 
CACGCCATGT ACTACCTCCG CTTGCTGATG ACTGAAGTGG CCTGGACTAA AGATGAATTA 
AAAGAAGCCT TGGATGATGT CACCCTTCCT CGCCTTAAGG CCTTCATACC TCAACTCCTG 
TCACGGCTGC ATATTGAAGC CCTTCTCCAT GGAAATATAA CAAAGCAGGC TGCGTTAGGA 
ATCATGCAGA TGGTTGAAGA CACCCTTATT GAACATGCTC ATACGAAACC ACTTCTTCCA 
AGTCAGCTCG TTCGGTACAG AGAAGTTCAG CTCCCGGACA GGGGATGGTT TGTGTATCAG 
CAGAGGAATG AAGTCCACAA TAACTGTGGC ATTGAGATAT ACTACCAAAC CGACATGCAG 
AGCACGTCAG AGAACATGTT TCTGGAGCTC TTCTGTCAGA TCATCTCCGA GCCTTGCTTC 
AACACCCTGC GCACCAAGGA GCAACTGGGC TATATCGTCT TCAGCGGGCC ACGCCGAGCC 
AATGGCATTC AGGGCTTACG GTTTATCATC CAGTCAGAAA AGCCACCTCA CTACCTAGAA 
AGCAGAGTGG AAGCTTTCTT AATTACCATG GAAAAGTCCA TAGAGGACAT GACAGAAGAG 
GCCTTCCAAA AACACATCCA AGCATTAGCA ATCCGTCGAC TAGACAAACC AAAGAAGCTA 
TCTGCAGAGT GTGCTAAATA TTGGGGGGAG ATCATCTCTC AGCAGTACAA TTTTGACAGA 
GATAATATAG AAGTTGCCTA TTTGAAGACA CTTACCAAGG AAGATATTAT CAAATTCTAT 
AAGGAAATGT TGGCGGTAGA TGCCCCGAGG AGACACAAGG TATCCGTCCA TGTTCTTGCC 
AGAGAAATGG ATTCTTGTCC TGTGGTTGGA GAGTTCCCAT GTCAAAATGA TATAAATTTG 
TCACAAGCAC CAGCCTTGCC ACAACCTGAA GTGATTCAGA ACATGACTGA GTTCAAGCGT 
GGTTTGCCAC TGTTCCCCCT TGTGAAACCA CATATTAACT TCATGGCTGC AAAACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IDE ( XM_010819775.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010818077.1 Bos taurus insulin-degrading enzyme (IDE), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa50774
Clone ID Related Accession (Same CDS sequence)	XM_010819775.2
Accession Version	XM_010819775.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3060bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	insulin-degrading enzyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104581.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010819775.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGTACC GGCTAGCGTG GCTGCTGCAT TCCGCGCTGC CCAGCACCTT CCGCTCGGTC 
CTCGGCGCCC GCCTGCCGCC CTCGGAGCGC CTCTGTGGTT TCCAAAAAAA GACTTACAGC 
AAAATGAATA ATCCAGCAAT CAAGAGAATA GGACATCATA TCATCAAGTC TCATGAAGAC 
AAGCGAGAGT ACCGCGGACT TGAGCTGGCC AATGGGATCA AAGTACTTCT TGTCAGTGAT 
CCAACAACAG ATAAATCATC AGCAGCACTT GATGTACACA TAGGTTCATT GTCAGATCCT 
CCAAATATTG CTGGCTTAAG TCATTTTTGT GAGCATATGT TATTTTTGGG AACAAAGAAA 
TACCCTAAAG AAAATGAATA CAGCCAGTTT CTCAGTGAAC ATGCAGGAAG TTCAAACGCC 
TTCACTAGTG GAGAGCATAC CAATTACTAT TTTGATGTTT CTCATGAACA CCTAGAAGGT 
GCGCTAGACA GATTTGCACA GTTTTTCCTG TGCCCCTTGT TTGATGAGAG CTGCAAAGAC 
AGAGAGGTGA ATGCAGTTGA TTCAGAACAT GAGAAGAATG TGATGAATGA TGCCTGGAGA 
CTCTTTCAGC TGGAAAAAGC TACAGGGAAT CCCAAACATC CCTTCAGTAA ATTTGGAACA 
GGTAACAAAT ATACTCTAGA GACTAGACCG AACCAAGAAG GCATTGATGT AAGACAAGAG 
CTCCTGAAAT TCCATTCTAC TTATTATTCA TCGAACTTAA TGGCTATTTG TGTTTTAGGT 
CGAGAATCTT TGGATGATTT GACTAATCTG GTGGTAAAGT TATTTTCTGA AGTAGAGAAC 
AAAAATGTCC CGTTGCCAGA ATTTCCTGAA CATCCTTTCC AAGAAGAACA TCTTAAACAA 
CTTTACAAAA TAGTACCCAT TAAGGATATT AGGAATCTTT ATGTGACATT TCCCATACCT 
GACCTTCAGA AATACTACAA ATCAAATCCC GGTCATTATC TCGGTCATCT CATTGGGCAT 
GAAGGTCCTG GAAGTCTGTT ATCAGAACTT AAATCAAAGG GCTGGGTAAA TACTCTTGTT 
GGTGGGCAAA AGGAAGGAGC CCGAGGTTTT ATGTTTTTTA TCATTAATGT GGACTTGACT 
GAGGAAGGAT TATTACATGT TGAGGATATA ATTTTGCACA TGTTTCAATA CATTCAGAAG 
TTACGAGCAG AAGGACCTCA AGAATGGGTT TTCCAAGAGT GCAAGGACTT GAATGCTGTT 
GCTTTTAGGT TTAAAGATAA AGAGAGACCA CGGGGTTATA CCTCTAAGAT TGCAGGAATA 
TTACATTATT ATCCCCTAGA AGAAGTGCTC ACAGCTGAAT ATTTACTGGA AGAATTTAGA 
CCAGATTTAA TAGAGATGGT TCTCGATAAA CTCAGACCAG AAAATGTCCG GGTTGCAATA 
GTTTCCAAAT CTTTTGAAGG AAAAACTGAT CGCACAGAAG AGTGGTACGG AACCCAGTAC 
AAACAAGAAG CTATACCAGA CGAAGTCATT AAGAAATGGC AAAATGCTGA TCTGAATGGG 
AAATTTAAAC TTCCAATGAA GAATGAATTT ATTCCTACAA ATTTTGAAAT TTTATCATTA 
GAAAAAGAAG CAACGCCATA CCCTTCTCTT ATTAAGGATA CAGCTATGAG CAAACTCTGG 
TTCAAACAAG ATGATAAGTT TTTCTTGCCC AAGGCCTGTC TCAACTTTGA ATTTTTCAGT 
CGCTACATTT ATGCTGATCC TCTCCATTGC AACATGACAT ACCTGTTTAT CAGGTTATTG 
AAGGATGATT TAAAAGAGTA TACATATGCA GCACGCCTCT CAGGTTTGAG CTATGGCATT 
GCATCAGGAA TGAATGCAAT ACTTCTCTCG GTGAAAGGTT ACAATGACAA GCAGCCAATT 
TTGCTAAAGA AGATCATTGA GAAAATGGCT ACCTTTGAGA TTGATGAAAA AAGATTTGAA 
ATTATCAAAG AGGCATATAT GCGATCTCTT AACAATTTCC GAGCTGAACA GCCTCATCAA 
CACGCCATGT ACTACCTCCG CTTGCTGATG ACTGAAGTGG CCTGGACTAA AGATGAATTA 
AAAGAAGCCT TGGATGATGT CACCCTTCCT CGCCTTAAGG CCTTCATACC TCAACTCCTG 
TCACGGCTGC ATATTGAAGC CCTTCTCCAT GGAAATATAA CAAAGCAGGC TGCGTTAGGA 
ATCATGCAGA TGGTTGAAGA CACCCTTATT GAACATGCTC ATACGAAACC ACTTCTTCCA 
AGTCAGCTCG TTCGGTACAG AGAAGTTCAG CTCCCGGACA GGGGATGGTT TGTGTATCAG 
CAGAGGAATG AAGTCCACAA TAACTGTGGC ATTGAGATAT ACTACCAAAC CGACATGCAG 
AGCACGTCAG AGAACATGTT TCTGGAGCTC TTCTGTCAGA TCATCTCCGA GCCTTGCTTC 
AACACCCTGC GCACCAAGGA GCAACTGGGC TATATCGTCT TCAGCGGGCC ACGCCGAGCC 
AATGGCATTC AGGGCTTACG GTTTATCATC CAGTCAGAAA AGCCACCTCA CTACCTAGAA 
AGCAGAGTGG AAGCTTTCTT AATTACCATG GAAAAGTCCA TAGAGGACAT GACAGAAGAG 
GCCTTCCAAA AACACATCCA AGCATTAGCA ATCCGTCGAC TAGACAAACC AAAGAAGCTA 
TCTGCAGAGT GTGCTAAATA TTGGGGGGAG ATCATCTCTC AGCAGTACAA TTTTGACAGA 
GATAATATAG AAGTTGCCTA TTTGAAGACA CTTACCAAGG AAGATATTAT CAAATTCTAT 
AAGGAAATGT TGGCGGTAGA TGCCCCGAGG AGACACAAGG TATCCGTCCA TGTTCTTGCC 
AGAGAAATGG ATTCTTGTCC TGTGGTTGGA GAGTTCCCAT GTCAAAATGA TATAAATTTG 
TCACAAGCAC CAGCCTTGCC ACAACCTGAA GTGATTCAGA ACATGACTGA GTTCAAGCGT 
GGTTTGCCAC TGTTCCCCCT TGTGAAACCA CATATTAACT TCATGGCTGC AAAACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010818077.1
CDS	67..3126
Translation	MRYRLAWLLHSALPSTFRSVLGARLPPSERLCGFQKKTYSKMNNPAIKRIGHHIIKSHEDKREYRGLELANGIKVLLVSDPTTDKSSAALDVHIGSLSDPPNIAGLSHFCEHMLFLGTKKYPKENEYSQFLSEHAGSSNAFTSGEHTNYYFDVSHEHLEGALDRFAQFFLCPLFDESCKDREVNAVDSEHEKNVMNDAWRLFQLEKATGNPKHPFSKFGTGNKYTLETRPNQEGIDVRQELLKFHSTYYSSNLMAICVLGRESLDDLTNLVVKLFSEVENKNVPLPEFPEHPFQEEHLKQLYKIVPIKDIRNLYVTFPIPDLQKYYKSNPGHYLGHLIGHEGPGSLLSELKSKGWVNTLVGGQKEGARGFMFFIINVDLTEEGLLHVEDIILHMFQYIQKLRAEGPQEWVFQECKDLNAVAFRFKDKERPRGYTSKIAGILHYYPLEEVLTAEYLLEEFRPDLIEMVLDKLRPENVRVAIVSKSFEGKTDRTEEWYGTQYKQEAIPDEVIKKWQNADLNGKFKLPMKNEFIPTNFEILSLEKEATPYPSLIKDTAMSKLWFKQDDKFFLPKACLNFEFFSRYIYADPLHCNMTYLFIRLLKDDLKEYTYAARLSGLSYGIASGMNAILLSVKGYNDKQPILLKKIIEKMATFEIDEKRFEIIKEAYMRSLNNFRAEQPHQHAMYYLRLLMTEVAWTKDELKEALDDVTLPRLKAFIPQLLSRLHIEALLHGNITKQAALGIMQMVEDTLIEHAHTKPLLPSQLVRYREVQLPDRGWFVYQQRNEVHNNCGIEIYYQTDMQSTSENMFLELFCQIISEPCFNTLRTKEQLGYIVFSGPRRANGIQGLRFIIQSEKPPHYLESRVEAFLITMEKSIEDMTEEAFQKHIQALAIRRLDKPKKLSAECAKYWGEIISQQYNFDRDNIEVAYLKTLTKEDIIKFYKEMLAVDAPRRHKVSVHVLAREMDSCPVVGEFPCQNDINLSQAPALPQPEVIQNMTEFKRGLPLFPLVKPHINFMAAKL

Target ORF information:

RefSeq Version	XM_010819775.2
Organism	Bos taurus(cattle)
Definition	Bos taurus insulin-degrading enzyme (IDE), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010819775.2

ORF Insert Sequence:

ATGCGGTACC GGCTAGCGTG GCTGCTGCAT TCCGCGCTGC CCAGCACCTT CCGCTCGGTC 
CTCGGCGCCC GCCTGCCGCC CTCGGAGCGC CTCTGTGGTT TCCAAAAAAA GACTTACAGC 
AAAATGAATA ATCCAGCAAT CAAGAGAATA GGACATCATA TCATCAAGTC TCATGAAGAC 
AAGCGAGAGT ACCGCGGACT TGAGCTGGCC AATGGGATCA AAGTACTTCT TGTCAGTGAT 
CCAACAACAG ATAAATCATC AGCAGCACTT GATGTACACA TAGGTTCATT GTCAGATCCT 
CCAAATATTG CTGGCTTAAG TCATTTTTGT GAGCATATGT TATTTTTGGG AACAAAGAAA 
TACCCTAAAG AAAATGAATA CAGCCAGTTT CTCAGTGAAC ATGCAGGAAG TTCAAACGCC 
TTCACTAGTG GAGAGCATAC CAATTACTAT TTTGATGTTT CTCATGAACA CCTAGAAGGT 
GCGCTAGACA GATTTGCACA GTTTTTCCTG TGCCCCTTGT TTGATGAGAG CTGCAAAGAC 
AGAGAGGTGA ATGCAGTTGA TTCAGAACAT GAGAAGAATG TGATGAATGA TGCCTGGAGA 
CTCTTTCAGC TGGAAAAAGC TACAGGGAAT CCCAAACATC CCTTCAGTAA ATTTGGAACA 
GGTAACAAAT ATACTCTAGA GACTAGACCG AACCAAGAAG GCATTGATGT AAGACAAGAG 
CTCCTGAAAT TCCATTCTAC TTATTATTCA TCGAACTTAA TGGCTATTTG TGTTTTAGGT 
CGAGAATCTT TGGATGATTT GACTAATCTG GTGGTAAAGT TATTTTCTGA AGTAGAGAAC 
AAAAATGTCC CGTTGCCAGA ATTTCCTGAA CATCCTTTCC AAGAAGAACA TCTTAAACAA 
CTTTACAAAA TAGTACCCAT TAAGGATATT AGGAATCTTT ATGTGACATT TCCCATACCT 
GACCTTCAGA AATACTACAA ATCAAATCCC GGTCATTATC TCGGTCATCT CATTGGGCAT 
GAAGGTCCTG GAAGTCTGTT ATCAGAACTT AAATCAAAGG GCTGGGTAAA TACTCTTGTT 
GGTGGGCAAA AGGAAGGAGC CCGAGGTTTT ATGTTTTTTA TCATTAATGT GGACTTGACT 
GAGGAAGGAT TATTACATGT TGAGGATATA ATTTTGCACA TGTTTCAATA CATTCAGAAG 
TTACGAGCAG AAGGACCTCA AGAATGGGTT TTCCAAGAGT GCAAGGACTT GAATGCTGTT 
GCTTTTAGGT TTAAAGATAA AGAGAGACCA CGGGGTTATA CCTCTAAGAT TGCAGGAATA 
TTACATTATT ATCCCCTAGA AGAAGTGCTC ACAGCTGAAT ATTTACTGGA AGAATTTAGA 
CCAGATTTAA TAGAGATGGT TCTCGATAAA CTCAGACCAG AAAATGTCCG GGTTGCAATA 
GTTTCCAAAT CTTTTGAAGG AAAAACTGAT CGCACAGAAG AGTGGTACGG AACCCAGTAC 
AAACAAGAAG CTATACCAGA CGAAGTCATT AAGAAATGGC AAAATGCTGA TCTGAATGGG 
AAATTTAAAC TTCCAATGAA GAATGAATTT ATTCCTACAA ATTTTGAAAT TTTATCATTA 
GAAAAAGAAG CAACGCCATA CCCTTCTCTT ATTAAGGATA CAGCTATGAG CAAACTCTGG 
TTCAAACAAG ATGATAAGTT TTTCTTGCCC AAGGCCTGTC TCAACTTTGA ATTTTTCAGT 
CGCTACATTT ATGCTGATCC TCTCCATTGC AACATGACAT ACCTGTTTAT CAGGTTATTG 
AAGGATGATT TAAAAGAGTA TACATATGCA GCACGCCTCT CAGGTTTGAG CTATGGCATT 
GCATCAGGAA TGAATGCAAT ACTTCTCTCG GTGAAAGGTT ACAATGACAA GCAGCCAATT 
TTGCTAAAGA AGATCATTGA GAAAATGGCT ACCTTTGAGA TTGATGAAAA AAGATTTGAA 
ATTATCAAAG AGGCATATAT GCGATCTCTT AACAATTTCC GAGCTGAACA GCCTCATCAA 
CACGCCATGT ACTACCTCCG CTTGCTGATG ACTGAAGTGG CCTGGACTAA AGATGAATTA 
AAAGAAGCCT TGGATGATGT CACCCTTCCT CGCCTTAAGG CCTTCATACC TCAACTCCTG 
TCACGGCTGC ATATTGAAGC CCTTCTCCAT GGAAATATAA CAAAGCAGGC TGCGTTAGGA 
ATCATGCAGA TGGTTGAAGA CACCCTTATT GAACATGCTC ATACGAAACC ACTTCTTCCA 
AGTCAGCTCG TTCGGTACAG AGAAGTTCAG CTCCCGGACA GGGGATGGTT TGTGTATCAG 
CAGAGGAATG AAGTCCACAA TAACTGTGGC ATTGAGATAT ACTACCAAAC CGACATGCAG 
AGCACGTCAG AGAACATGTT TCTGGAGCTC TTCTGTCAGA TCATCTCCGA GCCTTGCTTC 
AACACCCTGC GCACCAAGGA GCAACTGGGC TATATCGTCT TCAGCGGGCC ACGCCGAGCC 
AATGGCATTC AGGGCTTACG GTTTATCATC CAGTCAGAAA AGCCACCTCA CTACCTAGAA 
AGCAGAGTGG AAGCTTTCTT AATTACCATG GAAAAGTCCA TAGAGGACAT GACAGAAGAG 
GCCTTCCAAA AACACATCCA AGCATTAGCA ATCCGTCGAC TAGACAAACC AAAGAAGCTA 
TCTGCAGAGT GTGCTAAATA TTGGGGGGAG ATCATCTCTC AGCAGTACAA TTTTGACAGA 
GATAATATAG AAGTTGCCTA TTTGAAGACA CTTACCAAGG AAGATATTAT CAAATTCTAT 
AAGGAAATGT TGGCGGTAGA TGCCCCGAGG AGACACAAGG TATCCGTCCA TGTTCTTGCC 
AGAGAAATGG ATTCTTGTCC TGTGGTTGGA GAGTTCCCAT GTCAAAATGA TATAAATTTG 
TCACAAGCAC CAGCCTTGCC ACAACCTGAA GTGATTCAGA ACATGACTGA GTTCAAGCGT 
GGTTTGCCAC TGTTCCCCCT TGTGAAACCA CATATTAACT TCATGGCTGC AAAACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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IDE cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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