Ide cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ide
Entrez Gene ID	25700
Full Name	insulin degrading enzyme
Synonyms	INSDEGM
General protein information	Preferred Names insulin degrading enzyme Names insulin-degrading enzyme insulin protease insulinase insulysin
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q53
Summary	enzyme involved in the degradation of bioactive peptides [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_008760362.2	XP_008758584.1	insulin-degrading enzyme isoform X2
NM_013159.1	NP_037291.1	insulin-degrading enzyme
XM_006231313.3	XP_006231375.1	insulin-degrading enzyme isoform X1
XM_017588831.1	XP_017444320.1	insulin-degrading enzyme isoform X2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	IDE	NP_001069317.1
Danio rerio (zebrafish)	ide	NP_001082994.1
Caenorhabditis elegans (roundworm)	CELE_Y70C5C.1	NP_507226.2
Caenorhabditis elegans (roundworm)	C28F5.4	NP_495575.2
Kluyveromyces lactis	KLLA0E05105g	XP_454175.1
Magnaporthe oryzae (rice blast fungus)	MGG_13149	XP_003710564.1
Arabidopsis thaliana (thale cress)	AT3G57470	NP_001118852.1
Xenopus tropicalis (tropical clawed frog)	ide	XP_002939138.2
Drosophila melanogaster (fruit fly)	Ide	NP_524182.3
Caenorhabditis elegans (roundworm)	C02G6.2	NP_504531.2
Saccharomyces cerevisiae (baker's yeast)	STE23	NP_013493.2
Neurospora crassa	NCU00481	XP_956166.1
Homo sapiens (human)	IDE	NP_004960.2
Pan troglodytes (chimpanzee)	IDE	XP_507922.2
	IDE	XP_001090249.1
Canis lupus familiaris (dog)	IDE	XP_534963.3
Mus musculus (house mouse)	Ide	NP_112419.3
Rattus norvegicus (Norway rat)	Ide	NP_037291.1
Gallus gallus (chicken)	IDE	XP_421686.4
Caenorhabditis elegans (roundworm)	F44E7.4	NP_741542.1
Caenorhabditis elegans (roundworm)	C02G6.1	NP_504532.1
Schizosaccharomyces pombe (fission yeast)	mug138	NP_593966.1
Oryza sativa (rice)	Os07g0570300	NP_001060044.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010351	XP_311589.4
Eremothecium gossypii	AGOS_AER053C	NP_984913.2
Arabidopsis thaliana (thale cress)	AT2G41790	NP_181710.1

Gene Ontology
Bibliography

Related articles in PubMed

GLP-1 receptor regulates cell growth through regulating IDE expression level in A?1-42-treated PC12 cells.
Li H, Cao L, Ren Y, Jiang Y, Xie W, Li D
Bioscience reports(2017 Dec)

Neuroprotective mechanism of
Wang N, Jia YM, Zhang B, Xue D, Reeju M, Li Y, Huang SM, Liu XW
Neural regeneration research12(4)654-659(2017 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study concluded that vitamin D3 ameliorated insulin resistance and hyperinsulinemia in diabetic rat model received high fructose water through reduction of insulin-degrading enzyme and activation of insulin receptor phosphorylation.
Title: Vitamin D3 intake as regulator of insulin degrading enzyme and insulin receptor phosphorylation in diabetic rats.

Ageing and diabetes are accompanied by a deficit of IDE in the brain structures where accumulation of Abeta was reported in Azheimer disease patients.
Title: Effects of ageing and experimental diabetes on insulin-degrading enzyme?expression?in male rat tissues.

Insulin-degrading enzyme is able to degrade specific amino acid sequences present in the neuropeptide pro-NPFFA (NPFF precursor), generating some cryptic peptides that are also observed after incubation with rat brain cortex homogenate.
Title: Metabolism of cryptic peptides derived from neuropeptide FF precursors: the involvement of insulin-degrading enzyme.

IDE-Met(1) links the mitochondrial biogenesis pathway with mitAbeta levels and organelle functionality.
Title: Transcriptional regulation of insulin-degrading enzyme modulates mitochondrial amyloid ? (A?) peptide catabolism and functionality.

Proteolytically inactive insulin-degrading enzyme inhibits amyloid formation yielding non-neurotoxic abeta peptide aggregates.
Title: Proteolytically inactive insulin-degrading enzyme inhibits amyloid formation yielding non-neurotoxic a? peptide aggregates.

The following Ide gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ide cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa24052	XM_017588831.1 Latest version!	Rattus norvegicus insulin degrading enzyme (Ide), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa24052	XM_008760362.2 Latest version!	Rattus norvegicus insulin degrading enzyme (Ide), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa13554	NM_013159.1 Latest version!	Rattus norvegicus insulin degrading enzyme (Ide), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa24050	XM_006231313.3 Latest version!	Rattus norvegicus insulin degrading enzyme (Ide), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ide ( XM_008760362.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008758584.1 Rattus norvegicus insulin degrading enzyme (Ide), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa24052
Clone ID Related Accession (Same CDS sequence)	XM_008760362.2 , XM_017588831.1
Accession Version	XM_008760362.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2937bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	insulin-degrading enzyme isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008760362.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATAATC CGGCCATCCA GAGAATAGAA GACCATATTG TCAAGTCTCC TGAAGACAAA 
CGGGAATATC GTGGACTAGA ACTGGCCAAT GGTATCAAAG TGCTTCTCAT CAGTGATCCC 
ACCACGGACA AGTCATCAGC AGCACTCGAC GTGCACATAG GGTCACTGTC AGACCCTCCA 
AATATTCCTG GCTTAAGCCA TTTTTGTGAG CATATGCTGT TTTTGGGAAC AAAGAAATAT 
CCTAAGGAAA ATGAATACAG CCAGTTTCTC AGTGAACATG CTGGGAGTTC AAATGCATTT 
ACCAGCGGAG AACACACCAA TTATTATTTC GATGTTTCCC ATGAACACTT GGAAGGAGCC 
CTGGACAGGT TTGCCCAGTT TTTCCTGTGC CCCTTGTTTG ATGCAAGTTG TAAGGACAGA 
GAGGTGAACG CTGTCGATTC AGAGCATGAA AAGAATGTGA TGAATGATGC CTGGAGACTC 
TTCCAGCTGG AAAAGGCTAC AGGGAATCCC AAGCACCCCT TCAGCAAATT CGGGACAGGA 
AACAAATATA CTCTAGAGAC TCGACCCAAC CAAGAAGGCA TCGATGTAAG GGAAGAACTC 
TTGAAATTTC ACTCTACGTA TTATTCGTCC AATCTGATGG CGATTTGTGT TTTAGGTCGA 
GAATCTTTAG ATGACCTGAC TAATCTGGTG GTGAAGTTAT TTTCTGAAGT AGAGAATAAA 
AATGTCCCTT TGCCTGAATT TCCTGAGCAC CCTTTCCAAG AAGAACATCT TAAACAACTT 
TATAAAATAG TACCCATTAA GGATATTAGG AATCTTTATG TGACATTTCC CATACCAGAC 
CTTCAACAGT ACTACAAATC CAATCCCGGT CATTATCTCG GTCATCTGAT CGGGCATGAA 
GGTCCTGGAA GCCTGTTGTC AGAGCTCAAG TCAAAGGGCT GGGTAAACAC CCTGGTTGGG 
GGACAGAAGG AAGGAGCCCG AGGTTTTATG TTTTTTATCA TTAATGTGGA CTTAACTGAA 
GAAGGGTTAT TACATGTTGA AGATATAATT TTGCACATGT TTCAATACAT TCAGAAGCTA 
CGTGCTGAAG GACCTCAAGA ATGGGTTTTC CAAGAGTGCA AGGACTTGAA CGCTGTCGCT 
TTCAGGTTTA AAGATAAAGA GAGGCCACGA GGCTACACAT CTAAGATTGC AGGGAAATTG 
CACTATTATC CCCTAAATGG AGTGCTCACA GCTGAATATT TACTGGAAGA ATTTAGACCC 
GACTTGATAG ACATGGTTCT TGATAAACTC AGACCAGAAA ATGTCCGGGT GGCAATAGTT 
TCTAAATCAT TTGAAGGGAA AACTGACCGC ACAGAGCAGT GGTATGGAAC CCAGTACAAG 
CAAGAAGCTA TCCCAGAGGA CGTCATTCAG AAATGGCAAA ATGCTGACCT GAATGGGAAA 
TTTAAACTTC CAACAAAGAA TGAATTCATT CCTACAAATT TTGAGATTTT GGCATTAGAA 
AAAGATGCAA CACCATACCC TGCTCTTATT AAGGATACAG CCATGAGTAA GCTGTGGTTC 
AAACAAGATG ATAAATTTTT CTTGCCGAAA GCTTGTCTCA ACTTTGAATT TTTCAGCCCG 
TTTGCTTATG TGGACCCCTT GCACTGTAAC ATGGCCTATT TGTACCTTGA ACTCCTCAAA 
GACTCACTCA ACGAGTATGC ATATGCAGCA GAGCTAGCAG GCCTGAGCTA TGATCTCCAA 
AACACCATCT ATGGGATGTA TCTCTCAGTG AAAGGTTACA ATGACAAACA GCCAATTTTG 
CTAAAGAAGA TCACCGAGAA AATGGCTACT TTTGAGATTG ATAAAAAAAG ATTTGAAATT 
ATCAAAGAGG CGTACATGCG ATCTCTTAAT AATTTCCGGG CTGAGCAGCC TCACCAGCAC 
GCCATGTACT ACCTCCGTCT GCTGATGACT GAAGTGGCCT GGACCAAAGA TGAGTTAAAA 
GAAGCCCTCG ATGATGTGAC CCTCCCCCGT CTTAAGGCCT TCATACCTCA GCTGCTGTCC 
CGGCTGCATA TTGAAGCCCT TCTCCATGGC AACATAACAA AGCAGGCTGC CTTAGGAGTT 
ATGCAGATGG TAGAAGACAC CCTTATTGAG CATGCTCACA CCAAACCTCT CCTTCCAAGT 
CAGCTAGTCC GGTACAGAGA AGTTCAGCTC CCCGACCGAG GATGGTTTGT TTACCAGCGG 
AGGAACGAAG TCCACAATAA CTGTGGCATT GAGATTTACT ACCAGACAGA CATGCAGAGC 
ACCTCGGAGA ACATGTTCCT GGAGCTCTTC TGCCAGATTA TCTCTGAGCC TTGCTTCAAC 
ACTCTGCGTA CCAAGGAGCA GCTTGGCTAT ATTGTCTTCA GTGGACCTCG TCGGGCCAAC 
GGCATCCAGG GCTTGCGATT CATCATCCAG TCAGAAAAAC CACCTCACTA CCTGGAAAGC 
AGAGTGGAAG CCTTCTTGAT CACCATGGAA AAGGCCATAG AGGACATGAC AGAGGAGGCT 
TTCCAAAAAC ACATTCAGGC GTTAGCGATT CGCCGACTCG ACAAACCAAA GAAACTCTCT 
GCAGAGTGCG CGAAGTACTG GGGGGAGATC ATCTCCCAGC AGTACAATTA TGACAGAGAT 
AACATAGAGG TTGCATATTT AAAGACACTC AGCAAGGATG ATATCATCAA ATTCTACAAG 
GAAATGTTGG CTGTGGACGC ACCAAGGAGA CATAAAGTAT CCGTCCACGT TCTTGCCAGG 
GAAATGGATT CTTGTCCTGT GGTTGGAGAG TTCCCCTCTC AGAATGATAT AAACCTTTCC 
GAAGCGCCAC CCTTGCCACA ACCTGAGGTG ATTCATAACA TGACTGAATT CAAGCGCGGC 
CTGCCGCTGT TCCCCCTTGT GAAGCCACAC ATTAACTTCA TGGCGGCAAA ACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008758584.1
CDS	879..3815
Translation	MNNPAIQRIEDHIVKSPEDKREYRGLELANGIKVLLISDPTTDKSSAALDVHIGSLSDPPNIPGLSHFCEHMLFLGTKKYPKENEYSQFLSEHAGSSNAFTSGEHTNYYFDVSHEHLEGALDRFAQFFLCPLFDASCKDREVNAVDSEHEKNVMNDAWRLFQLEKATGNPKHPFSKFGTGNKYTLETRPNQEGIDVREELLKFHSTYYSSNLMAICVLGRESLDDLTNLVVKLFSEVENKNVPLPEFPEHPFQEEHLKQLYKIVPIKDIRNLYVTFPIPDLQQYYKSNPGHYLGHLIGHEGPGSLLSELKSKGWVNTLVGGQKEGARGFMFFIINVDLTEEGLLHVEDIILHMFQYIQKLRAEGPQEWVFQECKDLNAVAFRFKDKERPRGYTSKIAGKLHYYPLNGVLTAEYLLEEFRPDLIDMVLDKLRPENVRVAIVSKSFEGKTDRTEQWYGTQYKQEAIPEDVIQKWQNADLNGKFKLPTKNEFIPTNFEILALEKDATPYPALIKDTAMSKLWFKQDDKFFLPKACLNFEFFSPFAYVDPLHCNMAYLYLELLKDSLNEYAYAAELAGLSYDLQNTIYGMYLSVKGYNDKQPILLKKITEKMATFEIDKKRFEIIKEAYMRSLNNFRAEQPHQHAMYYLRLLMTEVAWTKDELKEALDDVTLPRLKAFIPQLLSRLHIEALLHGNITKQAALGVMQMVEDTLIEHAHTKPLLPSQLVRYREVQLPDRGWFVYQRRNEVHNNCGIEIYYQTDMQSTSENMFLELFCQIISEPCFNTLRTKEQLGYIVFSGPRRANGIQGLRFIIQSEKPPHYLESRVEAFLITMEKAIEDMTEEAFQKHIQALAIRRLDKPKKLSAECAKYWGEIISQQYNYDRDNIEVAYLKTLSKDDIIKFYKEMLAVDAPRRHKVSVHVLAREMDSCPVVGEFPSQNDINLSEAPPLPQPEVIHNMTEFKRGLPLFPLVKPHINFMAAKL

Target ORF information:

RefSeq Version	XM_008760362.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus insulin degrading enzyme (Ide), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008760362.2

ORF Insert Sequence:

ATGAATAATC CGGCCATCCA GAGAATAGAA GACCATATTG TCAAGTCTCC TGAAGACAAA 
CGGGAATATC GTGGACTAGA ACTGGCCAAT GGTATCAAAG TGCTTCTCAT CAGTGATCCC 
ACCACGGACA AGTCATCAGC AGCACTCGAC GTGCACATAG GGTCACTGTC AGACCCTCCA 
AATATTCCTG GCTTAAGCCA TTTTTGTGAG CATATGCTGT TTTTGGGAAC AAAGAAATAT 
CCTAAGGAAA ATGAATACAG CCAGTTTCTC AGTGAACATG CTGGGAGTTC AAATGCATTT 
ACCAGCGGAG AACACACCAA TTATTATTTC GATGTTTCCC ATGAACACTT GGAAGGAGCC 
CTGGACAGGT TTGCCCAGTT TTTCCTGTGC CCCTTGTTTG ATGCAAGTTG TAAGGACAGA 
GAGGTGAACG CTGTCGATTC AGAGCATGAA AAGAATGTGA TGAATGATGC CTGGAGACTC 
TTCCAGCTGG AAAAGGCTAC AGGGAATCCC AAGCACCCCT TCAGCAAATT CGGGACAGGA 
AACAAATATA CTCTAGAGAC TCGACCCAAC CAAGAAGGCA TCGATGTAAG GGAAGAACTC 
TTGAAATTTC ACTCTACGTA TTATTCGTCC AATCTGATGG CGATTTGTGT TTTAGGTCGA 
GAATCTTTAG ATGACCTGAC TAATCTGGTG GTGAAGTTAT TTTCTGAAGT AGAGAATAAA 
AATGTCCCTT TGCCTGAATT TCCTGAGCAC CCTTTCCAAG AAGAACATCT TAAACAACTT 
TATAAAATAG TACCCATTAA GGATATTAGG AATCTTTATG TGACATTTCC CATACCAGAC 
CTTCAACAGT ACTACAAATC CAATCCCGGT CATTATCTCG GTCATCTGAT CGGGCATGAA 
GGTCCTGGAA GCCTGTTGTC AGAGCTCAAG TCAAAGGGCT GGGTAAACAC CCTGGTTGGG 
GGACAGAAGG AAGGAGCCCG AGGTTTTATG TTTTTTATCA TTAATGTGGA CTTAACTGAA 
GAAGGGTTAT TACATGTTGA AGATATAATT TTGCACATGT TTCAATACAT TCAGAAGCTA 
CGTGCTGAAG GACCTCAAGA ATGGGTTTTC CAAGAGTGCA AGGACTTGAA CGCTGTCGCT 
TTCAGGTTTA AAGATAAAGA GAGGCCACGA GGCTACACAT CTAAGATTGC AGGGAAATTG 
CACTATTATC CCCTAAATGG AGTGCTCACA GCTGAATATT TACTGGAAGA ATTTAGACCC 
GACTTGATAG ACATGGTTCT TGATAAACTC AGACCAGAAA ATGTCCGGGT GGCAATAGTT 
TCTAAATCAT TTGAAGGGAA AACTGACCGC ACAGAGCAGT GGTATGGAAC CCAGTACAAG 
CAAGAAGCTA TCCCAGAGGA CGTCATTCAG AAATGGCAAA ATGCTGACCT GAATGGGAAA 
TTTAAACTTC CAACAAAGAA TGAATTCATT CCTACAAATT TTGAGATTTT GGCATTAGAA 
AAAGATGCAA CACCATACCC TGCTCTTATT AAGGATACAG CCATGAGTAA GCTGTGGTTC 
AAACAAGATG ATAAATTTTT CTTGCCGAAA GCTTGTCTCA ACTTTGAATT TTTCAGCCCG 
TTTGCTTATG TGGACCCCTT GCACTGTAAC ATGGCCTATT TGTACCTTGA ACTCCTCAAA 
GACTCACTCA ACGAGTATGC ATATGCAGCA GAGCTAGCAG GCCTGAGCTA TGATCTCCAA 
AACACCATCT ATGGGATGTA TCTCTCAGTG AAAGGTTACA ATGACAAACA GCCAATTTTG 
CTAAAGAAGA TCACCGAGAA AATGGCTACT TTTGAGATTG ATAAAAAAAG ATTTGAAATT 
ATCAAAGAGG CGTACATGCG ATCTCTTAAT AATTTCCGGG CTGAGCAGCC TCACCAGCAC 
GCCATGTACT ACCTCCGTCT GCTGATGACT GAAGTGGCCT GGACCAAAGA TGAGTTAAAA 
GAAGCCCTCG ATGATGTGAC CCTCCCCCGT CTTAAGGCCT TCATACCTCA GCTGCTGTCC 
CGGCTGCATA TTGAAGCCCT TCTCCATGGC AACATAACAA AGCAGGCTGC CTTAGGAGTT 
ATGCAGATGG TAGAAGACAC CCTTATTGAG CATGCTCACA CCAAACCTCT CCTTCCAAGT 
CAGCTAGTCC GGTACAGAGA AGTTCAGCTC CCCGACCGAG GATGGTTTGT TTACCAGCGG 
AGGAACGAAG TCCACAATAA CTGTGGCATT GAGATTTACT ACCAGACAGA CATGCAGAGC 
ACCTCGGAGA ACATGTTCCT GGAGCTCTTC TGCCAGATTA TCTCTGAGCC TTGCTTCAAC 
ACTCTGCGTA CCAAGGAGCA GCTTGGCTAT ATTGTCTTCA GTGGACCTCG TCGGGCCAAC 
GGCATCCAGG GCTTGCGATT CATCATCCAG TCAGAAAAAC CACCTCACTA CCTGGAAAGC 
AGAGTGGAAG CCTTCTTGAT CACCATGGAA AAGGCCATAG AGGACATGAC AGAGGAGGCT 
TTCCAAAAAC ACATTCAGGC GTTAGCGATT CGCCGACTCG ACAAACCAAA GAAACTCTCT 
GCAGAGTGCG CGAAGTACTG GGGGGAGATC ATCTCCCAGC AGTACAATTA TGACAGAGAT 
AACATAGAGG TTGCATATTT AAAGACACTC AGCAAGGATG ATATCATCAA ATTCTACAAG 
GAAATGTTGG CTGTGGACGC ACCAAGGAGA CATAAAGTAT CCGTCCACGT TCTTGCCAGG 
GAAATGGATT CTTGTCCTGT GGTTGGAGAG TTCCCCTCTC AGAATGATAT AAACCTTTCC 
GAAGCGCCAC CCTTGCCACA ACCTGAGGTG ATTCATAACA TGACTGAATT CAAGCGCGGC 
CTGCCGCTGT TCCCCCTTGT GAAGCCACAC ATTAACTTCA TGGCGGCAAA ACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ide ( XM_017588831.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017444320.1 Rattus norvegicus insulin degrading enzyme (Ide), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa24052
Clone ID Related Accession (Same CDS sequence)	XM_008760362.2 , XM_017588831.1
Accession Version	XM_017588831.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2937bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	insulin-degrading enzyme isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATAATC CGGCCATCCA GAGAATAGAA GACCATATTG TCAAGTCTCC TGAAGACAAA 
CGGGAATATC GTGGACTAGA ACTGGCCAAT GGTATCAAAG TGCTTCTCAT CAGTGATCCC 
ACCACGGACA AGTCATCAGC AGCACTCGAC GTGCACATAG GGTCACTGTC AGACCCTCCA 
AATATTCCTG GCTTAAGCCA TTTTTGTGAG CATATGCTGT TTTTGGGAAC AAAGAAATAT 
CCTAAGGAAA ATGAATACAG CCAGTTTCTC AGTGAACATG CTGGGAGTTC AAATGCATTT 
ACCAGCGGAG AACACACCAA TTATTATTTC GATGTTTCCC ATGAACACTT GGAAGGAGCC 
CTGGACAGGT TTGCCCAGTT TTTCCTGTGC CCCTTGTTTG ATGCAAGTTG TAAGGACAGA 
GAGGTGAACG CTGTCGATTC AGAGCATGAA AAGAATGTGA TGAATGATGC CTGGAGACTC 
TTCCAGCTGG AAAAGGCTAC AGGGAATCCC AAGCACCCCT TCAGCAAATT CGGGACAGGA 
AACAAATATA CTCTAGAGAC TCGACCCAAC CAAGAAGGCA TCGATGTAAG GGAAGAACTC 
TTGAAATTTC ACTCTACGTA TTATTCGTCC AATCTGATGG CGATTTGTGT TTTAGGTCGA 
GAATCTTTAG ATGACCTGAC TAATCTGGTG GTGAAGTTAT TTTCTGAAGT AGAGAATAAA 
AATGTCCCTT TGCCTGAATT TCCTGAGCAC CCTTTCCAAG AAGAACATCT TAAACAACTT 
TATAAAATAG TACCCATTAA GGATATTAGG AATCTTTATG TGACATTTCC CATACCAGAC 
CTTCAACAGT ACTACAAATC CAATCCCGGT CATTATCTCG GTCATCTGAT CGGGCATGAA 
GGTCCTGGAA GCCTGTTGTC AGAGCTCAAG TCAAAGGGCT GGGTAAACAC CCTGGTTGGG 
GGACAGAAGG AAGGAGCCCG AGGTTTTATG TTTTTTATCA TTAATGTGGA CTTAACTGAA 
GAAGGGTTAT TACATGTTGA AGATATAATT TTGCACATGT TTCAATACAT TCAGAAGCTA 
CGTGCTGAAG GACCTCAAGA ATGGGTTTTC CAAGAGTGCA AGGACTTGAA CGCTGTCGCT 
TTCAGGTTTA AAGATAAAGA GAGGCCACGA GGCTACACAT CTAAGATTGC AGGGAAATTG 
CACTATTATC CCCTAAATGG AGTGCTCACA GCTGAATATT TACTGGAAGA ATTTAGACCC 
GACTTGATAG ACATGGTTCT TGATAAACTC AGACCAGAAA ATGTCCGGGT GGCAATAGTT 
TCTAAATCAT TTGAAGGGAA AACTGACCGC ACAGAGCAGT GGTATGGAAC CCAGTACAAG 
CAAGAAGCTA TCCCAGAGGA CGTCATTCAG AAATGGCAAA ATGCTGACCT GAATGGGAAA 
TTTAAACTTC CAACAAAGAA TGAATTCATT CCTACAAATT TTGAGATTTT GGCATTAGAA 
AAAGATGCAA CACCATACCC TGCTCTTATT AAGGATACAG CCATGAGTAA GCTGTGGTTC 
AAACAAGATG ATAAATTTTT CTTGCCGAAA GCTTGTCTCA ACTTTGAATT TTTCAGCCCG 
TTTGCTTATG TGGACCCCTT GCACTGTAAC ATGGCCTATT TGTACCTTGA ACTCCTCAAA 
GACTCACTCA ACGAGTATGC ATATGCAGCA GAGCTAGCAG GCCTGAGCTA TGATCTCCAA 
AACACCATCT ATGGGATGTA TCTCTCAGTG AAAGGTTACA ATGACAAACA GCCAATTTTG 
CTAAAGAAGA TCACCGAGAA AATGGCTACT TTTGAGATTG ATAAAAAAAG ATTTGAAATT 
ATCAAAGAGG CGTACATGCG ATCTCTTAAT AATTTCCGGG CTGAGCAGCC TCACCAGCAC 
GCCATGTACT ACCTCCGTCT GCTGATGACT GAAGTGGCCT GGACCAAAGA TGAGTTAAAA 
GAAGCCCTCG ATGATGTGAC CCTCCCCCGT CTTAAGGCCT TCATACCTCA GCTGCTGTCC 
CGGCTGCATA TTGAAGCCCT TCTCCATGGC AACATAACAA AGCAGGCTGC CTTAGGAGTT 
ATGCAGATGG TAGAAGACAC CCTTATTGAG CATGCTCACA CCAAACCTCT CCTTCCAAGT 
CAGCTAGTCC GGTACAGAGA AGTTCAGCTC CCCGACCGAG GATGGTTTGT TTACCAGCGG 
AGGAACGAAG TCCACAATAA CTGTGGCATT GAGATTTACT ACCAGACAGA CATGCAGAGC 
ACCTCGGAGA ACATGTTCCT GGAGCTCTTC TGCCAGATTA TCTCTGAGCC TTGCTTCAAC 
ACTCTGCGTA CCAAGGAGCA GCTTGGCTAT ATTGTCTTCA GTGGACCTCG TCGGGCCAAC 
GGCATCCAGG GCTTGCGATT CATCATCCAG TCAGAAAAAC CACCTCACTA CCTGGAAAGC 
AGAGTGGAAG CCTTCTTGAT CACCATGGAA AAGGCCATAG AGGACATGAC AGAGGAGGCT 
TTCCAAAAAC ACATTCAGGC GTTAGCGATT CGCCGACTCG ACAAACCAAA GAAACTCTCT 
GCAGAGTGCG CGAAGTACTG GGGGGAGATC ATCTCCCAGC AGTACAATTA TGACAGAGAT 
AACATAGAGG TTGCATATTT AAAGACACTC AGCAAGGATG ATATCATCAA ATTCTACAAG 
GAAATGTTGG CTGTGGACGC ACCAAGGAGA CATAAAGTAT CCGTCCACGT TCTTGCCAGG 
GAAATGGATT CTTGTCCTGT GGTTGGAGAG TTCCCCTCTC AGAATGATAT AAACCTTTCC 
GAAGCGCCAC CCTTGCCACA ACCTGAGGTG ATTCATAACA TGACTGAATT CAAGCGCGGC 
CTGCCGCTGT TCCCCCTTGT GAAGCCACAC ATTAACTTCA TGGCGGCAAA ACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017444320.1
CDS	58..2994
Translation	MNNPAIQRIEDHIVKSPEDKREYRGLELANGIKVLLISDPTTDKSSAALDVHIGSLSDPPNIPGLSHFCEHMLFLGTKKYPKENEYSQFLSEHAGSSNAFTSGEHTNYYFDVSHEHLEGALDRFAQFFLCPLFDASCKDREVNAVDSEHEKNVMNDAWRLFQLEKATGNPKHPFSKFGTGNKYTLETRPNQEGIDVREELLKFHSTYYSSNLMAICVLGRESLDDLTNLVVKLFSEVENKNVPLPEFPEHPFQEEHLKQLYKIVPIKDIRNLYVTFPIPDLQQYYKSNPGHYLGHLIGHEGPGSLLSELKSKGWVNTLVGGQKEGARGFMFFIINVDLTEEGLLHVEDIILHMFQYIQKLRAEGPQEWVFQECKDLNAVAFRFKDKERPRGYTSKIAGKLHYYPLNGVLTAEYLLEEFRPDLIDMVLDKLRPENVRVAIVSKSFEGKTDRTEQWYGTQYKQEAIPEDVIQKWQNADLNGKFKLPTKNEFIPTNFEILALEKDATPYPALIKDTAMSKLWFKQDDKFFLPKACLNFEFFSPFAYVDPLHCNMAYLYLELLKDSLNEYAYAAELAGLSYDLQNTIYGMYLSVKGYNDKQPILLKKITEKMATFEIDKKRFEIIKEAYMRSLNNFRAEQPHQHAMYYLRLLMTEVAWTKDELKEALDDVTLPRLKAFIPQLLSRLHIEALLHGNITKQAALGVMQMVEDTLIEHAHTKPLLPSQLVRYREVQLPDRGWFVYQRRNEVHNNCGIEIYYQTDMQSTSENMFLELFCQIISEPCFNTLRTKEQLGYIVFSGPRRANGIQGLRFIIQSEKPPHYLESRVEAFLITMEKAIEDMTEEAFQKHIQALAIRRLDKPKKLSAECAKYWGEIISQQYNYDRDNIEVAYLKTLSKDDIIKFYKEMLAVDAPRRHKVSVHVLAREMDSCPVVGEFPSQNDINLSEAPPLPQPEVIHNMTEFKRGLPLFPLVKPHINFMAAKL

Target ORF information:

RefSeq Version	XM_017588831.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus insulin degrading enzyme (Ide), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017588831.1

ORF Insert Sequence:

ATGAATAATC CGGCCATCCA GAGAATAGAA GACCATATTG TCAAGTCTCC TGAAGACAAA 
CGGGAATATC GTGGACTAGA ACTGGCCAAT GGTATCAAAG TGCTTCTCAT CAGTGATCCC 
ACCACGGACA AGTCATCAGC AGCACTCGAC GTGCACATAG GGTCACTGTC AGACCCTCCA 
AATATTCCTG GCTTAAGCCA TTTTTGTGAG CATATGCTGT TTTTGGGAAC AAAGAAATAT 
CCTAAGGAAA ATGAATACAG CCAGTTTCTC AGTGAACATG CTGGGAGTTC AAATGCATTT 
ACCAGCGGAG AACACACCAA TTATTATTTC GATGTTTCCC ATGAACACTT GGAAGGAGCC 
CTGGACAGGT TTGCCCAGTT TTTCCTGTGC CCCTTGTTTG ATGCAAGTTG TAAGGACAGA 
GAGGTGAACG CTGTCGATTC AGAGCATGAA AAGAATGTGA TGAATGATGC CTGGAGACTC 
TTCCAGCTGG AAAAGGCTAC AGGGAATCCC AAGCACCCCT TCAGCAAATT CGGGACAGGA 
AACAAATATA CTCTAGAGAC TCGACCCAAC CAAGAAGGCA TCGATGTAAG GGAAGAACTC 
TTGAAATTTC ACTCTACGTA TTATTCGTCC AATCTGATGG CGATTTGTGT TTTAGGTCGA 
GAATCTTTAG ATGACCTGAC TAATCTGGTG GTGAAGTTAT TTTCTGAAGT AGAGAATAAA 
AATGTCCCTT TGCCTGAATT TCCTGAGCAC CCTTTCCAAG AAGAACATCT TAAACAACTT 
TATAAAATAG TACCCATTAA GGATATTAGG AATCTTTATG TGACATTTCC CATACCAGAC 
CTTCAACAGT ACTACAAATC CAATCCCGGT CATTATCTCG GTCATCTGAT CGGGCATGAA 
GGTCCTGGAA GCCTGTTGTC AGAGCTCAAG TCAAAGGGCT GGGTAAACAC CCTGGTTGGG 
GGACAGAAGG AAGGAGCCCG AGGTTTTATG TTTTTTATCA TTAATGTGGA CTTAACTGAA 
GAAGGGTTAT TACATGTTGA AGATATAATT TTGCACATGT TTCAATACAT TCAGAAGCTA 
CGTGCTGAAG GACCTCAAGA ATGGGTTTTC CAAGAGTGCA AGGACTTGAA CGCTGTCGCT 
TTCAGGTTTA AAGATAAAGA GAGGCCACGA GGCTACACAT CTAAGATTGC AGGGAAATTG 
CACTATTATC CCCTAAATGG AGTGCTCACA GCTGAATATT TACTGGAAGA ATTTAGACCC 
GACTTGATAG ACATGGTTCT TGATAAACTC AGACCAGAAA ATGTCCGGGT GGCAATAGTT 
TCTAAATCAT TTGAAGGGAA AACTGACCGC ACAGAGCAGT GGTATGGAAC CCAGTACAAG 
CAAGAAGCTA TCCCAGAGGA CGTCATTCAG AAATGGCAAA ATGCTGACCT GAATGGGAAA 
TTTAAACTTC CAACAAAGAA TGAATTCATT CCTACAAATT TTGAGATTTT GGCATTAGAA 
AAAGATGCAA CACCATACCC TGCTCTTATT AAGGATACAG CCATGAGTAA GCTGTGGTTC 
AAACAAGATG ATAAATTTTT CTTGCCGAAA GCTTGTCTCA ACTTTGAATT TTTCAGCCCG 
TTTGCTTATG TGGACCCCTT GCACTGTAAC ATGGCCTATT TGTACCTTGA ACTCCTCAAA 
GACTCACTCA ACGAGTATGC ATATGCAGCA GAGCTAGCAG GCCTGAGCTA TGATCTCCAA 
AACACCATCT ATGGGATGTA TCTCTCAGTG AAAGGTTACA ATGACAAACA GCCAATTTTG 
CTAAAGAAGA TCACCGAGAA AATGGCTACT TTTGAGATTG ATAAAAAAAG ATTTGAAATT 
ATCAAAGAGG CGTACATGCG ATCTCTTAAT AATTTCCGGG CTGAGCAGCC TCACCAGCAC 
GCCATGTACT ACCTCCGTCT GCTGATGACT GAAGTGGCCT GGACCAAAGA TGAGTTAAAA 
GAAGCCCTCG ATGATGTGAC CCTCCCCCGT CTTAAGGCCT TCATACCTCA GCTGCTGTCC 
CGGCTGCATA TTGAAGCCCT TCTCCATGGC AACATAACAA AGCAGGCTGC CTTAGGAGTT 
ATGCAGATGG TAGAAGACAC CCTTATTGAG CATGCTCACA CCAAACCTCT CCTTCCAAGT 
CAGCTAGTCC GGTACAGAGA AGTTCAGCTC CCCGACCGAG GATGGTTTGT TTACCAGCGG 
AGGAACGAAG TCCACAATAA CTGTGGCATT GAGATTTACT ACCAGACAGA CATGCAGAGC 
ACCTCGGAGA ACATGTTCCT GGAGCTCTTC TGCCAGATTA TCTCTGAGCC TTGCTTCAAC 
ACTCTGCGTA CCAAGGAGCA GCTTGGCTAT ATTGTCTTCA GTGGACCTCG TCGGGCCAAC 
GGCATCCAGG GCTTGCGATT CATCATCCAG TCAGAAAAAC CACCTCACTA CCTGGAAAGC 
AGAGTGGAAG CCTTCTTGAT CACCATGGAA AAGGCCATAG AGGACATGAC AGAGGAGGCT 
TTCCAAAAAC ACATTCAGGC GTTAGCGATT CGCCGACTCG ACAAACCAAA GAAACTCTCT 
GCAGAGTGCG CGAAGTACTG GGGGGAGATC ATCTCCCAGC AGTACAATTA TGACAGAGAT 
AACATAGAGG TTGCATATTT AAAGACACTC AGCAAGGATG ATATCATCAA ATTCTACAAG 
GAAATGTTGG CTGTGGACGC ACCAAGGAGA CATAAAGTAT CCGTCCACGT TCTTGCCAGG 
GAAATGGATT CTTGTCCTGT GGTTGGAGAG TTCCCCTCTC AGAATGATAT AAACCTTTCC 
GAAGCGCCAC CCTTGCCACA ACCTGAGGTG ATTCATAACA TGACTGAATT CAAGCGCGGC 
CTGCCGCTGT TCCCCCTTGT GAAGCCACAC ATTAACTTCA TGGCGGCAAA ACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ide ( NM_013159.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037291.1 Rattus norvegicus insulin degrading enzyme (Ide), mRNA.

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CloneID	ORa13554
Clone ID Related Accession (Same CDS sequence)	NM_013159.1
Accession Version	NM_013159.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3060bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	insulin-degrading enzyme
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X67269.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X67269.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGCGGAACG GGCTCGTGTG GCTGCTGCAC CCCGCGCTGC CCAGCACCTT GCACTCCATC 
CTCGGCGCTC GCCCGCCTCC CGTGAAGCGA CTGTGTGGAT TCCCAAAACA AATTTACAGC 
ACAATGAATA ATCCGGCCAT CCAGAGAATA GAAGACCATA TTGTCAAGTC TCCTGAAGAC 
AAACGGGAAT ATCGTGGACT AGAACTGGCC AATGGTATCA AAGTGCTTCT CATCAGTGAT 
CCCACCACGG ACAAGTCATC AGCAGCACTC GACGTGCACA TAGGGTCACT GTCAGACCCT 
CCAAATATTC CTGGCTTAAG CCATTTTTGT GAGCATATGC TGTTTTTGGG AACAAAGAAA 
TATCCTAAGG AAAATGAATA CAGCCAGTTT CTCAGTGAAC ATGCTGGGAG TTCAAATGCA 
TTTACCAGCG GAGAACACAC CAATTATTAT TTCGATGTTT CCCATGAACA CTTGGAAGGA 
GCCCTGGACA GGTTTGCCCA GTTTTTCCTG TGCCCCTTGT TTGATGCAAG TTGTAAGGAC 
AGAGAGGTGA ACGCTGTCGA TTCAGAGCAT GAAAAGAATG TGATGAATGA TGCCTGGAGA 
CTCTTCCAGC TGGAAAAGGC TACAGGGAAT CCCAAGCACC CCTTCAGCAA ATTCGGGACA 
GGAAACAAAT ATACTCTAGA GACTCGACCC AACCAAGAAG GCATCGATGT AAGGGAAGAA 
CTCTTGAAAT TTCACTCTAC GTATTATTCG TCCAATCTGA TGGCGATTTG TGTTTTAGGT 
CGAGAATCTT TAGATGACCT GACTAATCTG GTGGTGAAGT TATTTTCTGA AGTAGAGAAT 
AAAAATGTCC CTTTGCCTGA ATTTCCTGAG CACCCTTTCC AAGAAGAACA TCTTAAACAA 
CTTTATAAAA TAGTACCCAT TAAGGATATT AGGAATCTTT ATGTGACATT TCCCATACCA 
GACCTTCAAC AGTACTACAA ATCCAATCCC GGTCATTATC TCGGTCATCT GATCGGGCAT 
GAAGGTCCTG GAAGCCTGTT GTCAGAGCTC AAGTCAAAGG GCTGGGTAAA CACCCTGGTT 
GGGGGACAGA AGGAAGGAGC CCGAGGTTTT ATGTTTTTTA TCATTAATGT GGACTTAACT 
GAAGAAGGGT TATTACATGT TGAAGATATA ATTTTGCACA TGTTTCAATA CATTCAGAAG 
CTACGTGCTG AAGGACCTCA AGAATGGGTT TTCCAAGAGT GCAAGGACTT GAACGCTGTC 
GCTTTCAGGT TTAAAGATAA AGAGAGGCCA CGAGGCTACA CATCTAAGAT TGCAGGGAAA 
TTGCACTATT ATCCCCTAAA TGGAGTGCTC ACAGCTGAAT ATTTACTGGA AGAATTTAGA 
CCCGACTTGA TAGACATGGT TCTTGATAAA CTCAGACCAG AAAATGTCCG GGTGGCAATA 
GTTTCTAAAT CATTTGAAGG GAAAACTGAC CGCACAGAGC AGTGGTATGG AACCCAGTAC 
AAGCAAGAAG CTATCCCAGA GGACGTCATT CAGAAATGGC AAAATGCTGA CCTGAATGGG 
AAATTTAAAC TTCCAACAAA GAATGAATTC ATTCCTACAA ATTTTGAGAT TTTGGCATTA 
GAAAAAGATG CAACACCATA CCCTGCTCTT ATTAAGGATA CAGCCATGAG TAAGCTGTGG 
TTCAAACAAG ATGATAAATT TTTCTTGCCG AAAGCTTGTC TCAACTTTGA ATTTTTCAGC 
CCGTTTGCTT ATGTGGACCC CTTGCACTGT AACATGGCCT ATTTGTACCT TGAACTCCTC 
AAAGACTCAC TCAACGAGTA TGCATATGCA GCAGAGCTAG CAGGCCTGAG CTATGATCTC 
CAAAACACCA TCTATGGGAT GTATCTCTCA GTGAAAGGTT ACAATGACAA ACAGCCAATT 
TTGCTAAAGA AGATCACCGA GAAAATGGCT ACTTTTGAGA TTGATAAAAA AAGATTTGAA 
ATTATCAAAG AGGCGTACAT GCGATCTCTT AATAATTTCC GGGCTGAGCA GCCTCACCAG 
CACGCCATGT ACTACCTCCG TCTGCTGATG ACTGAAGTGG CCTGGACCAA AGATGAGTTA 
AAAGAAGCCC TCGATGATGT GACCCTCCCC CGTCTTAAGG CCTTCATACC TCAGCTGCTG 
TCCCGGCTGC ATATTGAAGC CCTTCTCCAT GGCAACATAA CAAAGCAGGC TGCCTTAGGA 
GTTATGCAGA TGGTAGAAGA CACCCTTATT GAGCATGCTC ACACCAAACC TCTCCTTCCA 
AGTCAGCTAG TCCGGTACAG AGAAGTTCAG CTCCCCGACC GAGGATGGTT TGTTTACCAG 
CGGAGGAACG AAGTCCACAA TAACTGTGGC ATTGAGATTT ACTACCAGAC AGACATGCAG 
AGCACCTCGG AGAACATGTT CCTGGAGCTC TTCTGCCAGA TTATCTCTGA GCCTTGCTTC 
AACACTCTGC GTACCAAGGA GCAGCTTGGC TATATTGTCT TCAGTGGACC TCGTCGGGCC 
AACGGCATCC AGGGCTTGCG ATTCATCATC CAGTCAGAAA AACCACCTCA CTACCTGGAA 
AGCAGAGTGG AAGCCTTCTT GATCACCATG GAAAAGGCCA TAGAGGACAT GACAGAGGAG 
GCTTTCCAAA AACACATTCA GGCGTTAGCG ATTCGCCGAC TCGACAAACC AAAGAAACTC 
TCTGCAGAGT GCGCGAAGTA CTGGGGGGAG ATCATCTCCC AGCAGTACAA TTATGACAGA 
GATAACATAG AGGTTGCATA TTTAAAGACA CTCAGCAAGG ATGATATCAT CAAATTCTAC 
AAGGAAATGT TGGCTGTGGA CGCACCAAGG AGACATAAAG TATCCGTCCA CGTTCTTGCC 
AGGGAAATGG ATTCTTGTCC TGTGGTTGGA GAGTTCCCCT CTCAGAATGA TATAAACCTT 
TCCGAAGCGC CACCCTTGCC ACAACCTGAG GTGATTCATA ACATGACTGA ATTCAAGCGC 
GGCCTGCCGC TGTTCCCCCT TGTGAAGCCA CACATTAACT TCATGGCGGC AAAACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037291.1
CDS	16..3075
Translation	MRNGLVWLLHPALPSTLHSILGARPPPVKRLCGFPKQIYSTMNNPAIQRIEDHIVKSPEDKREYRGLELANGIKVLLISDPTTDKSSAALDVHIGSLSDPPNIPGLSHFCEHMLFLGTKKYPKENEYSQFLSEHAGSSNAFTSGEHTNYYFDVSHEHLEGALDRFAQFFLCPLFDASCKDREVNAVDSEHEKNVMNDAWRLFQLEKATGNPKHPFSKFGTGNKYTLETRPNQEGIDVREELLKFHSTYYSSNLMAICVLGRESLDDLTNLVVKLFSEVENKNVPLPEFPEHPFQEEHLKQLYKIVPIKDIRNLYVTFPIPDLQQYYKSNPGHYLGHLIGHEGPGSLLSELKSKGWVNTLVGGQKEGARGFMFFIINVDLTEEGLLHVEDIILHMFQYIQKLRAEGPQEWVFQECKDLNAVAFRFKDKERPRGYTSKIAGKLHYYPLNGVLTAEYLLEEFRPDLIDMVLDKLRPENVRVAIVSKSFEGKTDRTEQWYGTQYKQEAIPEDVIQKWQNADLNGKFKLPTKNEFIPTNFEILALEKDATPYPALIKDTAMSKLWFKQDDKFFLPKACLNFEFFSPFAYVDPLHCNMAYLYLELLKDSLNEYAYAAELAGLSYDLQNTIYGMYLSVKGYNDKQPILLKKITEKMATFEIDKKRFEIIKEAYMRSLNNFRAEQPHQHAMYYLRLLMTEVAWTKDELKEALDDVTLPRLKAFIPQLLSRLHIEALLHGNITKQAALGVMQMVEDTLIEHAHTKPLLPSQLVRYREVQLPDRGWFVYQRRNEVHNNCGIEIYYQTDMQSTSENMFLELFCQIISEPCFNTLRTKEQLGYIVFSGPRRANGIQGLRFIIQSEKPPHYLESRVEAFLITMEKAIEDMTEEAFQKHIQALAIRRLDKPKKLSAECAKYWGEIISQQYNYDRDNIEVAYLKTLSKDDIIKFYKEMLAVDAPRRHKVSVHVLAREMDSCPVVGEFPSQNDINLSEAPPLPQPEVIHNMTEFKRGLPLFPLVKPHINFMAAKL

Target ORF information:

RefSeq Version	NM_013159.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus insulin degrading enzyme (Ide), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013159.1

ORF Insert Sequence:

ATGCGGAACG GGCTCGTGTG GCTGCTGCAC CCCGCGCTGC CCAGCACCTT GCACTCCATC 
CTCGGCGCTC GCCCGCCTCC CGTGAAGCGA CTGTGTGGAT TCCCAAAACA AATTTACAGC 
ACAATGAATA ATCCGGCCAT CCAGAGAATA GAAGACCATA TTGTCAAGTC TCCTGAAGAC 
AAACGGGAAT ATCGTGGACT AGAACTGGCC AATGGTATCA AAGTGCTTCT CATCAGTGAT 
CCCACCACGG ACAAGTCATC AGCAGCACTC GACGTGCACA TAGGGTCACT GTCAGACCCT 
CCAAATATTC CTGGCTTAAG CCATTTTTGT GAGCATATGC TGTTTTTGGG AACAAAGAAA 
TATCCTAAGG AAAATGAATA CAGCCAGTTT CTCAGTGAAC ATGCTGGGAG TTCAAATGCA 
TTTACCAGCG GAGAACACAC CAATTATTAT TTCGATGTTT CCCATGAACA CTTGGAAGGA 
GCCCTGGACA GGTTTGCCCA GTTTTTCCTG TGCCCCTTGT TTGATGCAAG TTGTAAGGAC 
AGAGAGGTGA ACGCTGTCGA TTCAGAGCAT GAAAAGAATG TGATGAATGA TGCCTGGAGA 
CTCTTCCAGC TGGAAAAGGC TACAGGGAAT CCCAAGCACC CCTTCAGCAA ATTCGGGACA 
GGAAACAAAT ATACTCTAGA GACTCGACCC AACCAAGAAG GCATCGATGT AAGGGAAGAA 
CTCTTGAAAT TTCACTCTAC GTATTATTCG TCCAATCTGA TGGCGATTTG TGTTTTAGGT 
CGAGAATCTT TAGATGACCT GACTAATCTG GTGGTGAAGT TATTTTCTGA AGTAGAGAAT 
AAAAATGTCC CTTTGCCTGA ATTTCCTGAG CACCCTTTCC AAGAAGAACA TCTTAAACAA 
CTTTATAAAA TAGTACCCAT TAAGGATATT AGGAATCTTT ATGTGACATT TCCCATACCA 
GACCTTCAAC AGTACTACAA ATCCAATCCC GGTCATTATC TCGGTCATCT GATCGGGCAT 
GAAGGTCCTG GAAGCCTGTT GTCAGAGCTC AAGTCAAAGG GCTGGGTAAA CACCCTGGTT 
GGGGGACAGA AGGAAGGAGC CCGAGGTTTT ATGTTTTTTA TCATTAATGT GGACTTAACT 
GAAGAAGGGT TATTACATGT TGAAGATATA ATTTTGCACA TGTTTCAATA CATTCAGAAG 
CTACGTGCTG AAGGACCTCA AGAATGGGTT TTCCAAGAGT GCAAGGACTT GAACGCTGTC 
GCTTTCAGGT TTAAAGATAA AGAGAGGCCA CGAGGCTACA CATCTAAGAT TGCAGGGAAA 
TTGCACTATT ATCCCCTAAA TGGAGTGCTC ACAGCTGAAT ATTTACTGGA AGAATTTAGA 
CCCGACTTGA TAGACATGGT TCTTGATAAA CTCAGACCAG AAAATGTCCG GGTGGCAATA 
GTTTCTAAAT CATTTGAAGG GAAAACTGAC CGCACAGAGC AGTGGTATGG AACCCAGTAC 
AAGCAAGAAG CTATCCCAGA GGACGTCATT CAGAAATGGC AAAATGCTGA CCTGAATGGG 
AAATTTAAAC TTCCAACAAA GAATGAATTC ATTCCTACAA ATTTTGAGAT TTTGGCATTA 
GAAAAAGATG CAACACCATA CCCTGCTCTT ATTAAGGATA CAGCCATGAG TAAGCTGTGG 
TTCAAACAAG ATGATAAATT TTTCTTGCCG AAAGCTTGTC TCAACTTTGA ATTTTTCAGC 
CCGTTTGCTT ATGTGGACCC CTTGCACTGT AACATGGCCT ATTTGTACCT TGAACTCCTC 
AAAGACTCAC TCAACGAGTA TGCATATGCA GCAGAGCTAG CAGGCCTGAG CTATGATCTC 
CAAAACACCA TCTATGGGAT GTATCTCTCA GTGAAAGGTT ACAATGACAA ACAGCCAATT 
TTGCTAAAGA AGATCACCGA GAAAATGGCT ACTTTTGAGA TTGATAAAAA AAGATTTGAA 
ATTATCAAAG AGGCGTACAT GCGATCTCTT AATAATTTCC GGGCTGAGCA GCCTCACCAG 
CACGCCATGT ACTACCTCCG TCTGCTGATG ACTGAAGTGG CCTGGACCAA AGATGAGTTA 
AAAGAAGCCC TCGATGATGT GACCCTCCCC CGTCTTAAGG CCTTCATACC TCAGCTGCTG 
TCCCGGCTGC ATATTGAAGC CCTTCTCCAT GGCAACATAA CAAAGCAGGC TGCCTTAGGA 
GTTATGCAGA TGGTAGAAGA CACCCTTATT GAGCATGCTC ACACCAAACC TCTCCTTCCA 
AGTCAGCTAG TCCGGTACAG AGAAGTTCAG CTCCCCGACC GAGGATGGTT TGTTTACCAG 
CGGAGGAACG AAGTCCACAA TAACTGTGGC ATTGAGATTT ACTACCAGAC AGACATGCAG 
AGCACCTCGG AGAACATGTT CCTGGAGCTC TTCTGCCAGA TTATCTCTGA GCCTTGCTTC 
AACACTCTGC GTACCAAGGA GCAGCTTGGC TATATTGTCT TCAGTGGACC TCGTCGGGCC 
AACGGCATCC AGGGCTTGCG ATTCATCATC CAGTCAGAAA AACCACCTCA CTACCTGGAA 
AGCAGAGTGG AAGCCTTCTT GATCACCATG GAAAAGGCCA TAGAGGACAT GACAGAGGAG 
GCTTTCCAAA AACACATTCA GGCGTTAGCG ATTCGCCGAC TCGACAAACC AAAGAAACTC 
TCTGCAGAGT GCGCGAAGTA CTGGGGGGAG ATCATCTCCC AGCAGTACAA TTATGACAGA 
GATAACATAG AGGTTGCATA TTTAAAGACA CTCAGCAAGG ATGATATCAT CAAATTCTAC 
AAGGAAATGT TGGCTGTGGA CGCACCAAGG AGACATAAAG TATCCGTCCA CGTTCTTGCC 
AGGGAAATGG ATTCTTGTCC TGTGGTTGGA GAGTTCCCCT CTCAGAATGA TATAAACCTT 
TCCGAAGCGC CACCCTTGCC ACAACCTGAG GTGATTCATA ACATGACTGA ATTCAAGCGC 
GGCCTGCCGC TGTTCCCCCT TGTGAAGCCA CACATTAACT TCATGGCGGC AAAACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ide ( XM_006231313.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006231375.1 Rattus norvegicus insulin degrading enzyme (Ide), transcript variant X1, mRNA.

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CloneID	ORa24050
Clone ID Related Accession (Same CDS sequence)	XM_006231313.3
Accession Version	XM_006231313.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3060bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	insulin-degrading enzyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006231313.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGAACG GGCTCGTGTG GCTGCTGCAC CCCGCGCTGC CCAGCACCTT GCACTCCATC 
CTCGGCGCTC GCCCGCCTCC CGTGAAGCGA CTGTGTGGAT TCCCAAAACA AATTTACAGC 
ACAATGAATA ATCCGGCCAT CCAGAGAATA GAAGACCATA TTGTCAAGTC TCCTGAAGAC 
AAACGGGAAT ATCGTGGACT AGAACTGGCC AATGGTATCA AAGTGCTTCT CATCAGTGAT 
CCCACCACGG ACAAGTCATC AGCAGCACTC GACGTGCACA TAGGGTCACT GTCAGACCCT 
CCAAATATTC CTGGCTTAAG CCATTTTTGT GAGCATATGC TGTTTTTGGG AACAAAGAAA 
TATCCTAAGG AAAATGAATA CAGCCAGTTT CTCAGTGAAC ATGCTGGGAG TTCAAATGCA 
TTTACCAGCG GAGAACACAC CAATTATTAT TTCGATGTTT CCCATGAACA CTTGGAAGGA 
GCCCTGGACA GGTTTGCCCA GTTTTTCCTG TGCCCCTTGT TTGATGCAAG TTGTAAGGAC 
AGAGAGGTGA ACGCTGTCGA TTCAGAGCAT GAAAAGAATG TGATGAATGA TGCCTGGAGA 
CTCTTCCAGC TGGAAAAGGC TACAGGGAAT CCCAAGCACC CCTTCAGCAA ATTCGGGACA 
GGAAACAAAT ATACTCTAGA GACTCGACCC AACCAAGAAG GCATCGATGT AAGGGAAGAA 
CTCTTGAAAT TTCACTCTAC GTATTATTCG TCCAATCTGA TGGCGATTTG TGTTTTAGGT 
CGAGAATCTT TAGATGACCT GACTAATCTG GTGGTGAAGT TATTTTCTGA AGTAGAGAAT 
AAAAATGTCC CTTTGCCTGA ATTTCCTGAG CACCCTTTCC AAGAAGAACA TCTTAAACAA 
CTTTATAAAA TAGTACCCAT TAAGGATATT AGGAATCTTT ATGTGACATT TCCCATACCA 
GACCTTCAAC AGTACTACAA ATCCAATCCC GGTCATTATC TCGGTCATCT GATCGGGCAT 
GAAGGTCCTG GAAGCCTGTT GTCAGAGCTC AAGTCAAAGG GCTGGGTAAA CACCCTGGTT 
GGGGGACAGA AGGAAGGAGC CCGAGGTTTT ATGTTTTTTA TCATTAATGT GGACTTAACT 
GAAGAAGGGT TATTACATGT TGAAGATATA ATTTTGCACA TGTTTCAATA CATTCAGAAG 
CTACGTGCTG AAGGACCTCA AGAATGGGTT TTCCAAGAGT GCAAGGACTT GAACGCTGTC 
GCTTTCAGGT TTAAAGATAA AGAGAGGCCA CGAGGCTACA CATCTAAGAT TGCAGGGAAA 
TTGCACTATT ATCCCCTAAA TGGAGTGCTC ACAGCTGAAT ATTTACTGGA AGAATTTAGA 
CCCGACTTGA TAGACATGGT TCTTGATAAA CTCAGACCAG AAAATGTCCG GGTGGCAATA 
GTTTCTAAAT CATTTGAAGG GAAAACTGAC CGCACAGAGC AGTGGTATGG AACCCAGTAC 
AAGCAAGAAG CTATCCCAGA GGACGTCATT CAGAAATGGC AAAATGCTGA CCTGAATGGG 
AAATTTAAAC TTCCAACAAA GAATGAATTC ATTCCTACAA ATTTTGAGAT TTTGGCATTA 
GAAAAAGATG CAACACCATA CCCTGCTCTT ATTAAGGATA CAGCCATGAG TAAGCTGTGG 
TTCAAACAAG ATGATAAATT TTTCTTGCCG AAAGCTTGTC TCAACTTTGA ATTTTTCAGT 
CGCTACATTT ATGCTGATCC TCTGCATTGC ACCATGACTT ACCTGTTTCT CAGGTTATTG 
AAGGATGATT TAAAAGAGTA TACATATGCA GCACGCCTGT CAGGTTTGAG CTATGCCATT 
GCCTCAGGGA TGAATGCAAT ACTTCTCTCA GTGAAAGGTT ACAATGACAA ACAGCCAATT 
TTGCTAAAGA AGATCACCGA GAAAATGGCT ACTTTTGAGA TTGATAAAAA AAGATTTGAA 
ATTATCAAAG AGGCGTACAT GCGATCTCTT AATAATTTCC GGGCTGAGCA GCCTCACCAG 
CACGCCATGT ACTACCTCCG TCTGCTGATG ACTGAAGTGG CCTGGACCAA AGATGAGTTA 
AAAGAAGCCC TCGATGATGT GACCCTCCCC CGTCTTAAGG CCTTCATACC TCAGCTGCTG 
TCCCGGCTGC ATATTGAAGC CCTTCTCCAT GGCAACATAA CAAAGCAGGC TGCCTTAGGA 
GTTATGCAGA TGGTAGAAGA CACCCTTATT GAGCATGCTC ACACCAAACC TCTCCTTCCA 
AGTCAGCTAG TCCGGTACAG AGAAGTTCAG CTCCCCGACC GAGGATGGTT TGTTTACCAG 
CGGAGGAACG AAGTCCACAA TAACTGTGGC ATTGAGATTT ACTACCAGAC AGACATGCAG 
AGCACCTCGG AGAACATGTT CCTGGAGCTC TTCTGCCAGA TTATCTCTGA GCCTTGCTTC 
AACACTCTGC GTACCAAGGA GCAGCTTGGC TATATTGTCT TCAGTGGACC TCGTCGGGCC 
AACGGCATCC AGGGCTTGCG ATTCATCATC CAGTCAGAAA AACCACCTCA CTACCTGGAA 
AGCAGAGTGG AAGCCTTCTT GATCACCATG GAAAAGGCCA TAGAGGACAT GACAGAGGAG 
GCTTTCCAAA AACACATTCA GGCGTTAGCG ATTCGCCGAC TCGACAAACC AAAGAAACTC 
TCTGCAGAGT GCGCGAAGTA CTGGGGGGAG ATCATCTCCC AGCAGTACAA TTATGACAGA 
GATAACATAG AGGTTGCATA TTTAAAGACA CTCAGCAAGG ATGATATCAT CAAATTCTAC 
AAGGAAATGT TGGCTGTGGA CGCACCAAGG AGACATAAAG TATCCGTCCA CGTTCTTGCC 
AGGGAAATGG ATTCTTGTCC TGTGGTTGGA GAGTTCCCCT CTCAGAATGA TATAAACCTT 
TCCGAAGCGC CACCCTTGCC ACAACCTGAG GTGATTCATA ACATGACTGA ATTCAAGCGC 
GGCCTGCCGC TGTTCCCCCT TGTGAAGCCA CACATTAACT TCATGGCGGC AAAACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006231375.1
CDS	690..3749
Translation	MRNGLVWLLHPALPSTLHSILGARPPPVKRLCGFPKQIYSTMNNPAIQRIEDHIVKSPEDKREYRGLELANGIKVLLISDPTTDKSSAALDVHIGSLSDPPNIPGLSHFCEHMLFLGTKKYPKENEYSQFLSEHAGSSNAFTSGEHTNYYFDVSHEHLEGALDRFAQFFLCPLFDASCKDREVNAVDSEHEKNVMNDAWRLFQLEKATGNPKHPFSKFGTGNKYTLETRPNQEGIDVREELLKFHSTYYSSNLMAICVLGRESLDDLTNLVVKLFSEVENKNVPLPEFPEHPFQEEHLKQLYKIVPIKDIRNLYVTFPIPDLQQYYKSNPGHYLGHLIGHEGPGSLLSELKSKGWVNTLVGGQKEGARGFMFFIINVDLTEEGLLHVEDIILHMFQYIQKLRAEGPQEWVFQECKDLNAVAFRFKDKERPRGYTSKIAGKLHYYPLNGVLTAEYLLEEFRPDLIDMVLDKLRPENVRVAIVSKSFEGKTDRTEQWYGTQYKQEAIPEDVIQKWQNADLNGKFKLPTKNEFIPTNFEILALEKDATPYPALIKDTAMSKLWFKQDDKFFLPKACLNFEFFSRYIYADPLHCTMTYLFLRLLKDDLKEYTYAARLSGLSYAIASGMNAILLSVKGYNDKQPILLKKITEKMATFEIDKKRFEIIKEAYMRSLNNFRAEQPHQHAMYYLRLLMTEVAWTKDELKEALDDVTLPRLKAFIPQLLSRLHIEALLHGNITKQAALGVMQMVEDTLIEHAHTKPLLPSQLVRYREVQLPDRGWFVYQRRNEVHNNCGIEIYYQTDMQSTSENMFLELFCQIISEPCFNTLRTKEQLGYIVFSGPRRANGIQGLRFIIQSEKPPHYLESRVEAFLITMEKAIEDMTEEAFQKHIQALAIRRLDKPKKLSAECAKYWGEIISQQYNYDRDNIEVAYLKTLSKDDIIKFYKEMLAVDAPRRHKVSVHVLAREMDSCPVVGEFPSQNDINLSEAPPLPQPEVIHNMTEFKRGLPLFPLVKPHINFMAAKL

Target ORF information:

RefSeq Version	XM_006231313.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus insulin degrading enzyme (Ide), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006231313.3

ORF Insert Sequence:

ATGCGGAACG GGCTCGTGTG GCTGCTGCAC CCCGCGCTGC CCAGCACCTT GCACTCCATC 
CTCGGCGCTC GCCCGCCTCC CGTGAAGCGA CTGTGTGGAT TCCCAAAACA AATTTACAGC 
ACAATGAATA ATCCGGCCAT CCAGAGAATA GAAGACCATA TTGTCAAGTC TCCTGAAGAC 
AAACGGGAAT ATCGTGGACT AGAACTGGCC AATGGTATCA AAGTGCTTCT CATCAGTGAT 
CCCACCACGG ACAAGTCATC AGCAGCACTC GACGTGCACA TAGGGTCACT GTCAGACCCT 
CCAAATATTC CTGGCTTAAG CCATTTTTGT GAGCATATGC TGTTTTTGGG AACAAAGAAA 
TATCCTAAGG AAAATGAATA CAGCCAGTTT CTCAGTGAAC ATGCTGGGAG TTCAAATGCA 
TTTACCAGCG GAGAACACAC CAATTATTAT TTCGATGTTT CCCATGAACA CTTGGAAGGA 
GCCCTGGACA GGTTTGCCCA GTTTTTCCTG TGCCCCTTGT TTGATGCAAG TTGTAAGGAC 
AGAGAGGTGA ACGCTGTCGA TTCAGAGCAT GAAAAGAATG TGATGAATGA TGCCTGGAGA 
CTCTTCCAGC TGGAAAAGGC TACAGGGAAT CCCAAGCACC CCTTCAGCAA ATTCGGGACA 
GGAAACAAAT ATACTCTAGA GACTCGACCC AACCAAGAAG GCATCGATGT AAGGGAAGAA 
CTCTTGAAAT TTCACTCTAC GTATTATTCG TCCAATCTGA TGGCGATTTG TGTTTTAGGT 
CGAGAATCTT TAGATGACCT GACTAATCTG GTGGTGAAGT TATTTTCTGA AGTAGAGAAT 
AAAAATGTCC CTTTGCCTGA ATTTCCTGAG CACCCTTTCC AAGAAGAACA TCTTAAACAA 
CTTTATAAAA TAGTACCCAT TAAGGATATT AGGAATCTTT ATGTGACATT TCCCATACCA 
GACCTTCAAC AGTACTACAA ATCCAATCCC GGTCATTATC TCGGTCATCT GATCGGGCAT 
GAAGGTCCTG GAAGCCTGTT GTCAGAGCTC AAGTCAAAGG GCTGGGTAAA CACCCTGGTT 
GGGGGACAGA AGGAAGGAGC CCGAGGTTTT ATGTTTTTTA TCATTAATGT GGACTTAACT 
GAAGAAGGGT TATTACATGT TGAAGATATA ATTTTGCACA TGTTTCAATA CATTCAGAAG 
CTACGTGCTG AAGGACCTCA AGAATGGGTT TTCCAAGAGT GCAAGGACTT GAACGCTGTC 
GCTTTCAGGT TTAAAGATAA AGAGAGGCCA CGAGGCTACA CATCTAAGAT TGCAGGGAAA 
TTGCACTATT ATCCCCTAAA TGGAGTGCTC ACAGCTGAAT ATTTACTGGA AGAATTTAGA 
CCCGACTTGA TAGACATGGT TCTTGATAAA CTCAGACCAG AAAATGTCCG GGTGGCAATA 
GTTTCTAAAT CATTTGAAGG GAAAACTGAC CGCACAGAGC AGTGGTATGG AACCCAGTAC 
AAGCAAGAAG CTATCCCAGA GGACGTCATT CAGAAATGGC AAAATGCTGA CCTGAATGGG 
AAATTTAAAC TTCCAACAAA GAATGAATTC ATTCCTACAA ATTTTGAGAT TTTGGCATTA 
GAAAAAGATG CAACACCATA CCCTGCTCTT ATTAAGGATA CAGCCATGAG TAAGCTGTGG 
TTCAAACAAG ATGATAAATT TTTCTTGCCG AAAGCTTGTC TCAACTTTGA ATTTTTCAGT 
CGCTACATTT ATGCTGATCC TCTGCATTGC ACCATGACTT ACCTGTTTCT CAGGTTATTG 
AAGGATGATT TAAAAGAGTA TACATATGCA GCACGCCTGT CAGGTTTGAG CTATGCCATT 
GCCTCAGGGA TGAATGCAAT ACTTCTCTCA GTGAAAGGTT ACAATGACAA ACAGCCAATT 
TTGCTAAAGA AGATCACCGA GAAAATGGCT ACTTTTGAGA TTGATAAAAA AAGATTTGAA 
ATTATCAAAG AGGCGTACAT GCGATCTCTT AATAATTTCC GGGCTGAGCA GCCTCACCAG 
CACGCCATGT ACTACCTCCG TCTGCTGATG ACTGAAGTGG CCTGGACCAA AGATGAGTTA 
AAAGAAGCCC TCGATGATGT GACCCTCCCC CGTCTTAAGG CCTTCATACC TCAGCTGCTG 
TCCCGGCTGC ATATTGAAGC CCTTCTCCAT GGCAACATAA CAAAGCAGGC TGCCTTAGGA 
GTTATGCAGA TGGTAGAAGA CACCCTTATT GAGCATGCTC ACACCAAACC TCTCCTTCCA 
AGTCAGCTAG TCCGGTACAG AGAAGTTCAG CTCCCCGACC GAGGATGGTT TGTTTACCAG 
CGGAGGAACG AAGTCCACAA TAACTGTGGC ATTGAGATTT ACTACCAGAC AGACATGCAG 
AGCACCTCGG AGAACATGTT CCTGGAGCTC TTCTGCCAGA TTATCTCTGA GCCTTGCTTC 
AACACTCTGC GTACCAAGGA GCAGCTTGGC TATATTGTCT TCAGTGGACC TCGTCGGGCC 
AACGGCATCC AGGGCTTGCG ATTCATCATC CAGTCAGAAA AACCACCTCA CTACCTGGAA 
AGCAGAGTGG AAGCCTTCTT GATCACCATG GAAAAGGCCA TAGAGGACAT GACAGAGGAG 
GCTTTCCAAA AACACATTCA GGCGTTAGCG ATTCGCCGAC TCGACAAACC AAAGAAACTC 
TCTGCAGAGT GCGCGAAGTA CTGGGGGGAG ATCATCTCCC AGCAGTACAA TTATGACAGA 
GATAACATAG AGGTTGCATA TTTAAAGACA CTCAGCAAGG ATGATATCAT CAAATTCTAC 
AAGGAAATGT TGGCTGTGGA CGCACCAAGG AGACATAAAG TATCCGTCCA CGTTCTTGCC 
AGGGAAATGG ATTCTTGTCC TGTGGTTGGA GAGTTCCCCT CTCAGAATGA TATAAACCTT 
TCCGAAGCGC CACCCTTGCC ACAACCTGAG GTGATTCATA ACATGACTGA ATTCAAGCGC 
GGCCTGCCGC TGTTCCCCCT TGTGAAGCCA CACATTAACT TCATGGCGGC AAAACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

GLP-1 receptor regulates cell growth through regulating IDE expression level in A?1-42-treated PC12 cells.
Bioscience reports(2017 Dec)
Li H,Cao L,Ren Y,Jiang Y,Xie W,Li D

Neuroprotective mechanism of
Neural regeneration research12(4)654-659(2017 Apr)
Wang N,Jia YM,Zhang B,Xue D,Reeju M,Li Y,Huang SM,Liu XW

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Ide cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

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