STE23 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	STE23
Entrez Gene ID	851105
Full Name	metalloendopeptidase
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001182278.1	NP_013493.2	metalloendopeptidase

Pathways from BioSystems

Homologies

Bos taurus (cattle)	IDE	NP_001069317.1
Danio rerio (zebrafish)	ide	NP_001082994.1
Caenorhabditis elegans (roundworm)	CELE_Y70C5C.1	NP_507226.2
Caenorhabditis elegans (roundworm)	C28F5.4	NP_495575.2
Kluyveromyces lactis	KLLA0E05105g	XP_454175.1
Magnaporthe oryzae (rice blast fungus)	MGG_13149	XP_003710564.1
Arabidopsis thaliana (thale cress)	AT3G57470	NP_001118852.1
Xenopus tropicalis (tropical clawed frog)	ide	XP_002939138.2
Drosophila melanogaster (fruit fly)	Ide	NP_524182.3
Caenorhabditis elegans (roundworm)	C02G6.2	NP_504531.2
Saccharomyces cerevisiae (baker's yeast)	STE23	NP_013493.2
Neurospora crassa	NCU00481	XP_956166.1
Homo sapiens (human)	IDE	NP_004960.2
Pan troglodytes (chimpanzee)	IDE	XP_507922.2
	IDE	XP_001090249.1
Canis lupus familiaris (dog)	IDE	XP_534963.3
Mus musculus (house mouse)	Ide	NP_112419.3
Rattus norvegicus (Norway rat)	Ide	NP_037291.1
Gallus gallus (chicken)	IDE	XP_421686.4
Caenorhabditis elegans (roundworm)	F44E7.4	NP_741542.1
Caenorhabditis elegans (roundworm)	C02G6.1	NP_504532.1
Schizosaccharomyces pombe (fission yeast)	mug138	NP_593966.1
Oryza sativa (rice)	Os07g0570300	NP_001060044.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010351	XP_311589.4
Eremothecium gossypii	AGOS_AER053C	NP_984913.2
Arabidopsis thaliana (thale cress)	AT2G41790	NP_181710.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
Johnston M, Hillier L, Riles L, Albermann K, Andr? B, Ansorge W, Benes V, Br?ckner M, Delius H, Dubois E, D?sterh?ft A, Entian KD, Floeth M, Goffeau A, Hebling U, Heumann K, Heuss-Neitzel D, Hilbert H, Hilger F, Kleine K, K?tter P, Louis EJ, Messenguy F, Mewes HW, Hoheisel JD
Nature387(6632 Suppl)87-90(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Authors identify the novel mitochondrial matrix protease Ste23 in yeast, a homologue of human insulin-degrading enzyme, which is required for efficient peptide degradation. Ste23 and Cym1 tightly cooperate to ensure the correct functioning of the essential presequence processing machinery.
Title: The novel mitochondrial matrix protease Ste23 is required for efficient presequence degradation and processing.

This study presents the first comprehensive biochemical analysis of a yeast M16A enzyme, and provides evidence that S. cerevisiae Ste23p has enzymatic properties that are highly consistent with mammalian IDEs and other M16A enzymes.
Title: Yeast Ste23p shares functional similarities with mammalian insulin-degrading enzymes.

The following STE23 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the STE23 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00704	NM_001182278.1 Latest version!	Saccharomyces cerevisiae S288C metalloendopeptidase (STE23), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

STE23 ( NM_001182278.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_013493.2 Saccharomyces cerevisiae S288C metalloendopeptidase (STE23), partial mRNA.

Price & Availability↑

CloneID	OSi00704
Clone ID Related Accession (Same CDS sequence)	NM_001182278.1
Accession Version	NM_001182278.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3084bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	metalloendopeptidase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001144). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGGCGTAT CTCTTTTAGC TTCTTCTTCT GCATTTGTAA CTAAACCACT CCTCACTCAA 
TTGGTTCACC TCTCACCTAT CAGTCTCAAT TTCACCGTAA GACGCTTCAA GCCATTCACG 
TGCTTATCAC GCTATTACAC AACAAATCCT TATAATATGA CATCCAATTT TAAGACATTC 
AATCTGGACT TTTTAAAGCC GGATTTAGAC GAAAGATCGT ATCGGTTTAT TGAGCTACCT 
AATAAGCTGA AGGCTCTGTT GATCCAGGAC CCAAAAGCAG ATAAAGCAGC AGCGTCGCTA 
GACGTCAATA TTGGCGCCTT TGAAGATCCA AAGAACCTAC CTGGATTGGC GCATTTCTGC 
GAGCATCTTT TATTCATGGG CTCTGAGAAA TTTCCAGACG AAAACGAATA CTCGAGCTAT 
TTAAGCAAGC ACGGTGGTTC ATCCAATGCC TACACAGCAT CGCAAAACAC AAATTATTTT 
TTTGAGGTTA ATCACCAACA TTTATTTGGT GCGCTAGACA GGTTTTCAGG GTTTTTCTCT 
TGTCCTCTTT TCAACAAGGA CTCTACTGAT AAAGAGATCA ACGCCGTTAA CAGCGAAAAC 
AAAAAGAACT TACAGAATGA TATTTGGAGA ATTTACCAAT TAGACAAGTC TTTGACCAAT 
ACAAAACATC CATATCACAA ATTTTCTACA GGAAACATCG AAACTTTGGG CACATTGCCA 
AAGGAAAATG GACTAAATGT TAGAGATGAA CTGTTAAAAT TCCATAAGAA TTTTTACTCC 
GCTAACCTAA TGAAGCTATG TATTCTTGGT AGAGAAGACC TTGATACTCT CTCTGATTGG 
ACTTATGATC TCTTCAAGGA TGTAGCCAAT AACGGTCGTG AAGTACCCCT TTATGCGGAA 
CCTATTATGC AGCCTGAGCA TTTGCAAAAA ATAATTCAAG TCCGTCCTGT GAAAGATTTG 
AAAAAACTAG AAATCTCTTT CACTGTCCCT GATATGGAGG AACATTGGGA GTCCAAGCCA 
CCGAGGATAT TAAGTCATTT AATCGGCCAT GAAGGGTCCG GTTCATTACT AGCACATTTG 
AAAAAATTGG GCTGGGCCAA TGAGCTATCT GCTGGTGGGC ATACTGTGTC CAAAGGAAAT 
GCTTTTTTTG CTGTCGATAT AGATTTAACA GACAACGGGT TGACACACTA TCGCGATGTT 
ATTGTCTTGA TCTTCCAGTA TATTGAAATG TTGAAAAACT CACTACCTCA AAAATGGATA 
TTCAATGAAC TGCAAGATAT CTCTAATGCT ACCTTCAAGT TTAAGCAAGC TGGTAGCCCA 
TCTTCCACAG TTTCATCATT GGCAAAATGC TTGGAAAAGG ATTACATTCC TGTTAGCAGA 
ATTTTAGCCA TGGGTTTGTT AACGAAATAT GAACCTGATT TGCTTACCCA ATATACGGAC 
GCTCTGGTCC CGGAAAATTC TCGTGTTACT CTGATCTCAA GGAGTTTAGA AACAGATTCT 
GCTGAAAAAT GGTATGGTAC TGCGTATAAA GTGGTAGATT ATCCGGCAGA TTTAATAAAA 
AACATGAAAT CGCCAGGTTT GAACCCTGCT CTGACTTTGC CACGTCCAAA TGAATTCGTT 
TCAACAAATT TCAAGGTTGA TAAAATTGAC GGTATAAAAC CATTAGATGA ACCTGTTTTG 
TTATTATCCG ATGACGTCAG CAAATTATGG TACAAGAAGG ACGACCGATT CTGGCAACCA 
CGAGGCTACA TTTACTTGTC TTTTAAACTT CCACATACGC ATGCAAGCAT CATTAATAGT 
ATGCTTTCTA CGCTATACAC TCAGCTGGCC AACGATGCCT TGAAAGATGT ACAATATGAT 
GCTGCTTGTG CCGATCTTCG TATATCATTC AATAAAACTA ATCAAGGCCT CGCCATTACC 
GCGTCTGGGT TCAACGAAAA ATTGATCATT CTATTAACAA GGTTTTTACA AGGCGTTAAT 
TCATTCGAAC CTAAGAAGGA TCGTTTTGAG ATTTTGAAAG ATAAGACCAT ACGTCACTTG 
AAAAACCTTT TATATGAAGT TCCTTATTCA CAAATGTCGA ATTACTACAA TGCTATAATA 
AATGAACGTT CTTGGTCAAC TGCAGAAAAA TTACAAGTCT TTGAAAAATT GACCTTTGAA 
CAATTGATCA ACTTCATACC CACCATTTAC GAAGGCGTTT ATTTTGAAAC TTTAATCCAC 
GGAAACATAA AACATGAAGA AGCATTGGAG GTGGATTCTC TGATCAAGTC TTTGATTCCA 
AACAACATTC ATAATCTGCA GGTTTCTAAC AACCGGTTGA GATCCTACTT ACTTCCTAAA 
GGCAAAACTT TCAGATATGA AACTGCTTTG AAAGACTCCC AGAATGTTAA CTCATGTATT 
CAACATGTCA CCCAGCTAGA TGTCTATTCA GAAGACCTAT CTGCCCTCAG CGGATTATTT 
GCGCAATTGA TTCACGAGCC ATGTTTTGAT ACCTTAAGAA CAAAAGAGCA ATTGGGCTAC 
GTTGTTTTTA GTTCGAGTTT GAACAATCAC GGTACAGCGA ACATTAGGAT CTTGATTCAG 
TCAGAACATA CCACGCCATA TCTAGAATGG AGAATAAACA ATTTTTATGA AACTTTTGGC 
CAGGTTTTGA GGGATATGCC AGAAGAGGAC TTTGAAAAAC ACAAAGAGGC GTTATGCAAC 
AGTCTATTGC AAAAATTCAA GAATATGGCC GAGGAAAGCG CCAGATACAC AGCAGCAATT 
TACTTAGGTG ATTACAATTT CACACATCGT CAAAAAAAGG CTAAGCTCGT TGCTAACATT 
ACAAAGCAGC AAATGATAGA TTTTTATGAG AATTATATAA TGAGCGAGAA CGCATCGAAG 
TTGATTCTAC ATTTGAAATC TCAAGTGGAA AACAAAGAGC TCAATGAAAA TGAATTGGAC 
ACCGCTAAGT ATCCAACTGG CCAGTTGATC GAAGATGTCG GGGCATTCAA GTCGACGCTG 
TTTGTAGCTC CCGTTCGTCA ACCAATGAAA GACTTCGAGA TTTCCGCGCC TCCTAAGCTG 
AATAATAGTT CTGAATCAGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_013493.2
CDS	1..3084
Translation	MGVSLLASSSAFVTKPLLTQLVHLSPISLNFTVRRFKPFTCLSRYYTTNPYNMTSNFKTFNLDFLKPDLDERSYRFIELPNKLKALLIQDPKADKAAASLDVNIGAFEDPKNLPGLAHFCEHLLFMGSEKFPDENEYSSYLSKHGGSSNAYTASQNTNYFFEVNHQHLFGALDRFSGFFSCPLFNKDSTDKEINAVNSENKKNLQNDIWRIYQLDKSLTNTKHPYHKFSTGNIETLGTLPKENGLNVRDELLKFHKNFYSANLMKLCILGREDLDTLSDWTYDLFKDVANNGREVPLYAEPIMQPEHLQKIIQVRPVKDLKKLEISFTVPDMEEHWESKPPRILSHLIGHEGSGSLLAHLKKLGWANELSAGGHTVSKGNAFFAVDIDLTDNGLTHYRDVIVLIFQYIEMLKNSLPQKWIFNELQDISNATFKFKQAGSPSSTVSSLAKCLEKDYIPVSRILAMGLLTKYEPDLLTQYTDALVPENSRVTLISRSLETDSAEKWYGTAYKVVDYPADLIKNMKSPGLNPALTLPRPNEFVSTNFKVDKIDGIKPLDEPVLLLSDDVSKLWYKKDDRFWQPRGYIYLSFKLPHTHASIINSMLSTLYTQLANDALKDVQYDAACADLRISFNKTNQGLAITASGFNEKLIILLTRFLQGVNSFEPKKDRFEILKDKTIRHLKNLLYEVPYSQMSNYYNAIINERSWSTAEKLQVFEKLTFEQLINFIPTIYEGVYFETLIHGNIKHEEALEVDSLIKSLIPNNIHNLQVSNNRLRSYLLPKGKTFRYETALKDSQNVNSCIQHVTQLDVYSEDLSALSGLFAQLIHEPCFDTLRTKEQLGYVVFSSSLNNHGTANIRILIQSEHTTPYLEWRINNFYETFGQVLRDMPEEDFEKHKEALCNSLLQKFKNMAEESARYTAAIYLGDYNFTHRQKKAKLVANITKQQMIDFYENYIMSENASKLILHLKSQVENKELNENELDTAKYPTGQLIEDVGAFKSTLFVAPVRQPMKDFEISAPPKLNNSSESE

Target ORF information:

RefSeq Version	NM_001182278.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C metalloendopeptidase (STE23), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001182278.1

ORF Insert Sequence:

ATGGGCGTAT CTCTTTTAGC TTCTTCTTCT GCATTTGTAA CTAAACCACT CCTCACTCAA 
TTGGTTCACC TCTCACCTAT CAGTCTCAAT TTCACCGTAA GACGCTTCAA GCCATTCACG 
TGCTTATCAC GCTATTACAC AACAAATCCT TATAATATGA CATCCAATTT TAAGACATTC 
AATCTGGACT TTTTAAAGCC GGATTTAGAC GAAAGATCGT ATCGGTTTAT TGAGCTACCT 
AATAAGCTGA AGGCTCTGTT GATCCAGGAC CCAAAAGCAG ATAAAGCAGC AGCGTCGCTA 
GACGTCAATA TTGGCGCCTT TGAAGATCCA AAGAACCTAC CTGGATTGGC GCATTTCTGC 
GAGCATCTTT TATTCATGGG CTCTGAGAAA TTTCCAGACG AAAACGAATA CTCGAGCTAT 
TTAAGCAAGC ACGGTGGTTC ATCCAATGCC TACACAGCAT CGCAAAACAC AAATTATTTT 
TTTGAGGTTA ATCACCAACA TTTATTTGGT GCGCTAGACA GGTTTTCAGG GTTTTTCTCT 
TGTCCTCTTT TCAACAAGGA CTCTACTGAT AAAGAGATCA ACGCCGTTAA CAGCGAAAAC 
AAAAAGAACT TACAGAATGA TATTTGGAGA ATTTACCAAT TAGACAAGTC TTTGACCAAT 
ACAAAACATC CATATCACAA ATTTTCTACA GGAAACATCG AAACTTTGGG CACATTGCCA 
AAGGAAAATG GACTAAATGT TAGAGATGAA CTGTTAAAAT TCCATAAGAA TTTTTACTCC 
GCTAACCTAA TGAAGCTATG TATTCTTGGT AGAGAAGACC TTGATACTCT CTCTGATTGG 
ACTTATGATC TCTTCAAGGA TGTAGCCAAT AACGGTCGTG AAGTACCCCT TTATGCGGAA 
CCTATTATGC AGCCTGAGCA TTTGCAAAAA ATAATTCAAG TCCGTCCTGT GAAAGATTTG 
AAAAAACTAG AAATCTCTTT CACTGTCCCT GATATGGAGG AACATTGGGA GTCCAAGCCA 
CCGAGGATAT TAAGTCATTT AATCGGCCAT GAAGGGTCCG GTTCATTACT AGCACATTTG 
AAAAAATTGG GCTGGGCCAA TGAGCTATCT GCTGGTGGGC ATACTGTGTC CAAAGGAAAT 
GCTTTTTTTG CTGTCGATAT AGATTTAACA GACAACGGGT TGACACACTA TCGCGATGTT 
ATTGTCTTGA TCTTCCAGTA TATTGAAATG TTGAAAAACT CACTACCTCA AAAATGGATA 
TTCAATGAAC TGCAAGATAT CTCTAATGCT ACCTTCAAGT TTAAGCAAGC TGGTAGCCCA 
TCTTCCACAG TTTCATCATT GGCAAAATGC TTGGAAAAGG ATTACATTCC TGTTAGCAGA 
ATTTTAGCCA TGGGTTTGTT AACGAAATAT GAACCTGATT TGCTTACCCA ATATACGGAC 
GCTCTGGTCC CGGAAAATTC TCGTGTTACT CTGATCTCAA GGAGTTTAGA AACAGATTCT 
GCTGAAAAAT GGTATGGTAC TGCGTATAAA GTGGTAGATT ATCCGGCAGA TTTAATAAAA 
AACATGAAAT CGCCAGGTTT GAACCCTGCT CTGACTTTGC CACGTCCAAA TGAATTCGTT 
TCAACAAATT TCAAGGTTGA TAAAATTGAC GGTATAAAAC CATTAGATGA ACCTGTTTTG 
TTATTATCCG ATGACGTCAG CAAATTATGG TACAAGAAGG ACGACCGATT CTGGCAACCA 
CGAGGCTACA TTTACTTGTC TTTTAAACTT CCACATACGC ATGCAAGCAT CATTAATAGT 
ATGCTTTCTA CGCTATACAC TCAGCTGGCC AACGATGCCT TGAAAGATGT ACAATATGAT 
GCTGCTTGTG CCGATCTTCG TATATCATTC AATAAAACTA ATCAAGGCCT CGCCATTACC 
GCGTCTGGGT TCAACGAAAA ATTGATCATT CTATTAACAA GGTTTTTACA AGGCGTTAAT 
TCATTCGAAC CTAAGAAGGA TCGTTTTGAG ATTTTGAAAG ATAAGACCAT ACGTCACTTG 
AAAAACCTTT TATATGAAGT TCCTTATTCA CAAATGTCGA ATTACTACAA TGCTATAATA 
AATGAACGTT CTTGGTCAAC TGCAGAAAAA TTACAAGTCT TTGAAAAATT GACCTTTGAA 
CAATTGATCA ACTTCATACC CACCATTTAC GAAGGCGTTT ATTTTGAAAC TTTAATCCAC 
GGAAACATAA AACATGAAGA AGCATTGGAG GTGGATTCTC TGATCAAGTC TTTGATTCCA 
AACAACATTC ATAATCTGCA GGTTTCTAAC AACCGGTTGA GATCCTACTT ACTTCCTAAA 
GGCAAAACTT TCAGATATGA AACTGCTTTG AAAGACTCCC AGAATGTTAA CTCATGTATT 
CAACATGTCA CCCAGCTAGA TGTCTATTCA GAAGACCTAT CTGCCCTCAG CGGATTATTT 
GCGCAATTGA TTCACGAGCC ATGTTTTGAT ACCTTAAGAA CAAAAGAGCA ATTGGGCTAC 
GTTGTTTTTA GTTCGAGTTT GAACAATCAC GGTACAGCGA ACATTAGGAT CTTGATTCAG 
TCAGAACATA CCACGCCATA TCTAGAATGG AGAATAAACA ATTTTTATGA AACTTTTGGC 
CAGGTTTTGA GGGATATGCC AGAAGAGGAC TTTGAAAAAC ACAAAGAGGC GTTATGCAAC 
AGTCTATTGC AAAAATTCAA GAATATGGCC GAGGAAAGCG CCAGATACAC AGCAGCAATT 
TACTTAGGTG ATTACAATTT CACACATCGT CAAAAAAAGG CTAAGCTCGT TGCTAACATT 
ACAAAGCAGC AAATGATAGA TTTTTATGAG AATTATATAA TGAGCGAGAA CGCATCGAAG 
TTGATTCTAC ATTTGAAATC TCAAGTGGAA AACAAAGAGC TCAATGAAAA TGAATTGGAC 
ACCGCTAAGT ATCCAACTGG CCAGTTGATC GAAGATGTCG GGGCATTCAA GTCGACGCTG 
TTTGTAGCTC CCGTTCGTCA ACCAATGAAA GACTTCGAGA TTTCCGCGCC TCCTAAGCTG 
AATAATAGTT CTGAATCAGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
Nature387(6632 Suppl)87-90(1997 May)
Johnston M,Hillier L,Riles L,Albermann K,Andr? B,Ansorge W,Benes V,Br?ckner M,Delius H,Dubois E,D?sterh?ft A,Entian KD,Floeth M,Goffeau A,Hebling U,Heumann K,Heuss-Neitzel D,Hilbert H,Hilger F,Kleine K,K?tter P,Louis EJ,Messenguy F,Mewes HW,Hoheisel JD

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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STE23 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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