ASXL1 cDNA ORF clone, Bos taurus(cattle)

The following ASXL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ASXL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa222038 XM_025001188.1
Latest version!
Bos taurus additional sex combs like 1, transcriptional regulator (ASXL1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa92396 XM_015474264.1
Latest version!
Bos taurus additional sex combs like 1, transcriptional regulator (ASXL1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OBa92396 XM_015466141.1
Latest version!
Bos taurus additional sex combs like 1, transcriptional regulator (ASXL1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OBa222035 XM_025001184.1
Latest version!
Bos taurus additional sex combs like 1, transcriptional regulator (ASXL1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa222036 XM_025001185.1
Latest version!
Bos taurus additional sex combs like 1, transcriptional regulator (ASXL1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa222037 XM_025001186.1
Latest version!
Bos taurus additional sex combs like 1, transcriptional regulator (ASXL1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa07672 XM_600364.7
Latest version!
Bos taurus additional sex combs like 1, transcriptional regulator (ASXL1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa07672 XM_002692288.5
Latest version!
Bos taurus additional sex combs like 1, transcriptional regulator (ASXL1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
View Old Accession Versions >>
OBa07672 XM_002692288.4 Bos taurus additional sex combs like 1, transcriptional regulator (ASXL1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa222038
    Clone ID Related Accession (Same CDS sequence) XM_025001188.1
    Accession Version XM_025001188.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4455bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product putative Polycomb group protein ASXL1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAAGGACA AACAGAAGAG GAAGAAGGAG CGCACGTGGG CCGAGGCCGC GCGCCTGGTA 
    CTGGAAAACT ATTCAGATGC TCCAATGACA CCCAAACAGA TTCTGCAGGT CATAGAGGCA
    GAAGGACTAA AGGAAATGAG AAGTGGGACA TCCCCTCTCG CATGTCTTAA TGCCATGCTA
    CATTCCAATT CCAGAGGAGG AGAGGGGTTA TTTTATAAAC TACCTGGCCG AATCAGCCTT
    TTTACACTCA AGAAGGATGC CCTGCAGTGG TCTCGCAGTC CAGCTGTGGT GGAGGGAGAG
    GAGCCAGAGG ACACAGCTGA TGTGGAGAGC TGTGGGTCTA ATGAAGCCAG CACTGTGAGT
    GGTGAAAATG ATGTTTCTCT CGATGAAACA TCTTCTAATG CTTCCTGTTC TACGGAATCT
    CAGAGTCGGC CTCTTTCCAA CCCCAGGGAC AGCTGCAGAG CTTCCTCACA GGCAAACAAG
    CAGAAGAAAA AGACAGGGGT GATGCTGCCT CGAGTTGTCC TGACTCCTCT GAAGGTAAAC
    GGGGCCCACG TGGAATCAGC ATCAGGTCAA ATGAAGCGCA ACAGAGGGGA AGAGATCGAT
    TTTGAGACGC CTGGGTCCAT TCTCGTCAAC ACCAACCTCC GGGCCCTGAT CAACTCGCGG
    ACCTTCCATG CCCTGCCGTC CCACTTCCAG CAGCAGCTCC TCTTCCTCCT GCCCGAAGTA
    GACAGACAGG TGGGAACTGA TGGCCTGTTA CGTCTCAGCA GTAGTGCGCT GAACAATGAG
    TTTTTCACCC ACGCAGCTCA GAGCTGGCGG GAACGCCTGG CTGATGGTGA ATTCACTCAT
    GAGATGCAGG TCAGGATACG ACAAGAAATG GAGAAGGAAA AGAAAGTGGA ACAATGGAAA
    GAAAAGTTTT TCGAAGACTA CTATGGACAG AAGCTGGGTT TAACCAAAGA AGAGTCATTG
    CAGCAGAATG TGGGCCAGGA GGAGGCTGAA ATCAAGAGTA ACCTGCGTGT CTCAGAATCA
    ACACGGCCAC AGCGTGGCCC GGCCACCCGG CAGCGAGATG GGCATTTCAA GAAACGCTCT
    CGGCCAGATC TTCGAACCAG AGCCCGAAGG AATCTGTACA AAAGACAGGA GCCAGAACAA
    GCAGAGATCG CTAAAGACGC ACAGTCTGTG ACACTAGACA TCCCCCTCCA CAAGGACGGG
    GAGGCTAAGA CCGACGCAGC AGGGGTGGGC AGCCCCCACC CACCTGGCAC GTCCTCCGCA
    GCATCCAACC CAGAGAGTCC CGAATTCCCA GTGGAAACTG TGGCCTCCCG GCTCCAGCCC
    AGTCCCAATG ACCAGGCACG TGTCTCCGCG TCTCCAGACA GAATTCCCAG CTTGCTGCAG
    GAGACCGTGG ATCAGGAACC CAAGGATCAG AAGAGGAAAT CCTTTGAGCA GGCGACCTCT
    GCCTCCTTTC CCGAAAAGAA GCCCCGGCTT GAAGATCGTC AGTCCTTTCG TAACACAATT
    GAAAGTGTTC ACCCCGAAAA GCCACAGCCC ACCAAAGAGG AGCCCAAAGT CCCGCCCATC
    CGGATTCAAC TTTCACGTAT CAAACCACCC TGGGTGGTTA AAGGTCAGCC CACTTACCAG
    ATATGTCCCC GCATCGTCCC CATCACGGAG TCCTCCTGCC GGGGCTGGAC TGGTGCCAGG
    ACCCTTGCAG ACATTAAAGC CCGTGCTCTG CAGGTCCGAG GGGCGAGAGG CCACCACTGC
    CATCGAGAGG CGGCCACCAC TGCCATCGGA GGTGGGGGTG GCCCGGGTGG AGGTGGCGGC
    GGGGCCACCG ATGAGGGAGG TGGCAGAGGC GGCGGCAGTG GTGATGGTGG TGAGGCCTGT
    GGCCACCCTG AGCCCCGGGG GGCCCCGAGT GCCCCTGGAG AGTGTGCGTC AGATCTACAG
    CGAACACAAC TACTGCCGCC TTGTCCTCTA AACGGGGATC ACGCCCAGGC CGAAACTGCT
    GCGTCCAGAG CCAGGAGAGA GGACCTGGCT TCTCTCAGAA AGGGGCAGAG CTGCCCACTG
    CAGAGGGTCC CAGATGGCCC TAGCGGCGGG CTGGAAGATG CCTCCCGACT CCCCGTTGCA
    TCCACTCGGG ATCCGCCATG CCAGGCTCTG CCCCCACTGC CCTCTAGAAT CCCAGAACCT
    GAGAGGTTAG TTGAGCAGCT TGTGTCGCAT CCAGAAGGTA GAACTGAATG TGGGTCTGGT
    ACCACTGCCT GGGAAAGGGG TGATGAGAAG CTAGCACCTA CCATCCCCTC AGAGAACAGT
    CCTGTTCGGG CTCTGGCCGG GCCTACTGGA TTAGAAGAAG GAACTGGCCA GGCTCCAGAC
    AGTGGCAGTA ATCCCACCAT GAAGGATCCT GTGAACGTGA CCCCCAGTTC CATCTCCGAA
    TCATCCTTGG CCAGTTGCCT GCAAGACAGA CCATTTGATG ATGAATCAGA ACTTGATGAC
    TCGGGCCCAC CCACAAGGGA AAGTTCTTCT AGACAGGAAA ACTTGAAAAC CGAGGCTCCT
    GTCTCCCCTG GTGCTGCTCC TTGGAGGCCT GGCATGTCAA ATGATGAGGC AGGGGGACAA
    CCTGAACCCG ACTCCAGAGA AGAGGTCCCG TCCATTAAGT CCCAGGTCAG AGAGAAGTGG
    GAGAAAGCGG CCCCCCTCAT CCCAGCATCG CCTGTGGGCT TGACAGCGGA GGAGGGTCTG
    GATCCCCCCG TCCACTTGGG TTCACTCTGG ACAGTGCACT CTGGCTGTGT TGACAGCAGC
    GGTGACTGCA GACAGCTGGA AGGTGACAAG CCCAGAATCA ATGGAGACTC GGAAGCTCTG
    AGTCCTCACA GCGAGTCCAC AGACACTGCC TCTGACTTCG AAGGCCACTT CTCCGAGGAC
    AGCAGTGAGG CCGAGCCTGG TGAAACCTTG GGGCCGAAGA GGTCCTTGGT GGCTGAGCAG
    GGTGAGAAAC ACAGTTGGAA CCACTGTGCC TCGCTCTCCA AGGTGAATGG TGACCTGAGT
    CTGGTCACCA GGACAGATGG GATGGTTGCT CCTCAGAGCT GGGTGTCTCG GGTATGTGCA
    GTTCCCCCAA AGATCCCAGA CTCCCTGCTG CTGGCCAGTC CCGAGTACCA GCCGAGACCC
    ATGTCCCTGG GCAGGCCTGC ATCCTCAGTG GAGGCTGCTA ACCCCCTTGT GATGCAGTTG
    CTGCAGGGCC ACTTGCCCCT CAAGAAGGTT CTCCCTCCAG CCCATGGCGG CAGCAAACCC
    GAACCCCCAC GACTCGCACT TACAGCAGAG CAGAGCCCTG GTGGCTCCCT GGGGATGGGG
    TCATTGCAAG GTCCTGGGGA GAACAGATGT GAAGTTGGCA AGAGCAGTCC AGAGTCTCTG
    CTACCTGAGA GCTGTGAAGC AAGTACCGGC TTTGCCCGGC TGGAGGCTAG CCAGGCTCCT
    GGGGCGCCCC TAAAGATGTC CAAGAACGCC CCGAGTTTAG ACTCCCTATA TCCAGTGACC
    AATCCAGTGG CTGCCTCTGG GAAAGCAGAA GTGGATTTCA AAGAACAGTT ATCTCCCTTC
    AGTTTTGAAG ATCAGAAGGA AGCCCGTAAC CTGTCCCAGT GCAGTATTTC AACTGCTGCC
    CCAGGTGTGA GTCTGGGAAA TCTCACTACC TCGAGAGCCC CTCTTTTCTC ATCTCCAAAT
    GCGGTCTCCT CGGGTCCTGA TCAGGCAGGT CGGGCCCTGG GGGATCAGAA CAGTGCGGGA
    GGCCAAGGGA AGAAGCTCTT TGGCTCCAAG AACGCAGCTG CAGCCCTTCA GTGCCCCCGG
    CTGGTGGAGC CGACGCCACT GCCTGCGGAG GCCCCTCCTG CTTTTCCCAA TAGGAAGTCA
    GGGCCAGGCA AAACCTCTCT GTCTGGTGGG ATACAGACTG CCAGGGAAGA CTGGGCCCCA
    AAGCCACCGC CTGCCTCTGT TGGCAGCATC AAGAGCGAAA AGACTCTTGA TGGAGGGCCT
    CTCAAGGTGG ATGCAGAGAA CAGAAAGGCA GTGGGGCCTG GTCCCCGGGA GCTGGTGGAT
    CACTTGCAAG GGATGCCCTT TGTCCTTGAT TTGCCCTTCT GGAAATTACC CCGGGAGCCC
    GGGAAGGGAC TCAGTCAGCC TCTGGAGCCT TCTTCCATCC CCTCCCAACT CAACATCAAG
    CAGGCATTTT ATGGGAAGCT TTCCAAACTC CAGCTAAGTT CCACCAGCTT TAATTATTCC
    TCCGGCTCCC CCCCTTTTCC CAAAGGCCTG GCCGGAAGTG TGGTGCAGCT GAGCCACAAA
    GCAAACTTTG GTGCGAGCCA CAGTGCGTCC CTCTCCTTGC AAATGTTCAC CGACAGCAGC
    ACAGTGGAAA GCATCTCGCT CCAGTGTGCG TGCAGCCTGA AAGCCATGAT CATGTGCCAG
    GGCTGCGGTG CATTCTGTCA CGATGACTGT ATTGGACCCT CAAAGCTCTG TGTATTGTGC
    CTTGTGGTGA GATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024856956.1
    CDS72..4526
    Translation

    Target ORF information:

    RefSeq Version XM_025001188.1
    Organism Bos taurus(cattle)
    Definition Bos taurus additional sex combs like 1, transcriptional regulator (ASXL1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025001188.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAAGGACA AACAGAAGAG GAAGAAGGAG CGCACGTGGG CCGAGGCCGC GCGCCTGGTA 
    CTGGAAAACT ATTCAGATGC TCCAATGACA CCCAAACAGA TTCTGCAGGT CATAGAGGCA
    GAAGGACTAA AGGAAATGAG AAGTGGGACA TCCCCTCTCG CATGTCTTAA TGCCATGCTA
    CATTCCAATT CCAGAGGAGG AGAGGGGTTA TTTTATAAAC TACCTGGCCG AATCAGCCTT
    TTTACACTCA AGAAGGATGC CCTGCAGTGG TCTCGCAGTC CAGCTGTGGT GGAGGGAGAG
    GAGCCAGAGG ACACAGCTGA TGTGGAGAGC TGTGGGTCTA ATGAAGCCAG CACTGTGAGT
    GGTGAAAATG ATGTTTCTCT CGATGAAACA TCTTCTAATG CTTCCTGTTC TACGGAATCT
    CAGAGTCGGC CTCTTTCCAA CCCCAGGGAC AGCTGCAGAG CTTCCTCACA GGCAAACAAG
    CAGAAGAAAA AGACAGGGGT GATGCTGCCT CGAGTTGTCC TGACTCCTCT GAAGGTAAAC
    GGGGCCCACG TGGAATCAGC ATCAGGTCAA ATGAAGCGCA ACAGAGGGGA AGAGATCGAT
    TTTGAGACGC CTGGGTCCAT TCTCGTCAAC ACCAACCTCC GGGCCCTGAT CAACTCGCGG
    ACCTTCCATG CCCTGCCGTC CCACTTCCAG CAGCAGCTCC TCTTCCTCCT GCCCGAAGTA
    GACAGACAGG TGGGAACTGA TGGCCTGTTA CGTCTCAGCA GTAGTGCGCT GAACAATGAG
    TTTTTCACCC ACGCAGCTCA GAGCTGGCGG GAACGCCTGG CTGATGGTGA ATTCACTCAT
    GAGATGCAGG TCAGGATACG ACAAGAAATG GAGAAGGAAA AGAAAGTGGA ACAATGGAAA
    GAAAAGTTTT TCGAAGACTA CTATGGACAG AAGCTGGGTT TAACCAAAGA AGAGTCATTG
    CAGCAGAATG TGGGCCAGGA GGAGGCTGAA ATCAAGAGTA ACCTGCGTGT CTCAGAATCA
    ACACGGCCAC AGCGTGGCCC GGCCACCCGG CAGCGAGATG GGCATTTCAA GAAACGCTCT
    CGGCCAGATC TTCGAACCAG AGCCCGAAGG AATCTGTACA AAAGACAGGA GCCAGAACAA
    GCAGAGATCG CTAAAGACGC ACAGTCTGTG ACACTAGACA TCCCCCTCCA CAAGGACGGG
    GAGGCTAAGA CCGACGCAGC AGGGGTGGGC AGCCCCCACC CACCTGGCAC GTCCTCCGCA
    GCATCCAACC CAGAGAGTCC CGAATTCCCA GTGGAAACTG TGGCCTCCCG GCTCCAGCCC
    AGTCCCAATG ACCAGGCACG TGTCTCCGCG TCTCCAGACA GAATTCCCAG CTTGCTGCAG
    GAGACCGTGG ATCAGGAACC CAAGGATCAG AAGAGGAAAT CCTTTGAGCA GGCGACCTCT
    GCCTCCTTTC CCGAAAAGAA GCCCCGGCTT GAAGATCGTC AGTCCTTTCG TAACACAATT
    GAAAGTGTTC ACCCCGAAAA GCCACAGCCC ACCAAAGAGG AGCCCAAAGT CCCGCCCATC
    CGGATTCAAC TTTCACGTAT CAAACCACCC TGGGTGGTTA AAGGTCAGCC CACTTACCAG
    ATATGTCCCC GCATCGTCCC CATCACGGAG TCCTCCTGCC GGGGCTGGAC TGGTGCCAGG
    ACCCTTGCAG ACATTAAAGC CCGTGCTCTG CAGGTCCGAG GGGCGAGAGG CCACCACTGC
    CATCGAGAGG CGGCCACCAC TGCCATCGGA GGTGGGGGTG GCCCGGGTGG AGGTGGCGGC
    GGGGCCACCG ATGAGGGAGG TGGCAGAGGC GGCGGCAGTG GTGATGGTGG TGAGGCCTGT
    GGCCACCCTG AGCCCCGGGG GGCCCCGAGT GCCCCTGGAG AGTGTGCGTC AGATCTACAG
    CGAACACAAC TACTGCCGCC TTGTCCTCTA AACGGGGATC ACGCCCAGGC CGAAACTGCT
    GCGTCCAGAG CCAGGAGAGA GGACCTGGCT TCTCTCAGAA AGGGGCAGAG CTGCCCACTG
    CAGAGGGTCC CAGATGGCCC TAGCGGCGGG CTGGAAGATG CCTCCCGACT CCCCGTTGCA
    TCCACTCGGG ATCCGCCATG CCAGGCTCTG CCCCCACTGC CCTCTAGAAT CCCAGAACCT
    GAGAGGTTAG TTGAGCAGCT TGTGTCGCAT CCAGAAGGTA GAACTGAATG TGGGTCTGGT
    ACCACTGCCT GGGAAAGGGG TGATGAGAAG CTAGCACCTA CCATCCCCTC AGAGAACAGT
    CCTGTTCGGG CTCTGGCCGG GCCTACTGGA TTAGAAGAAG GAACTGGCCA GGCTCCAGAC
    AGTGGCAGTA ATCCCACCAT GAAGGATCCT GTGAACGTGA CCCCCAGTTC CATCTCCGAA
    TCATCCTTGG CCAGTTGCCT GCAAGACAGA CCATTTGATG ATGAATCAGA ACTTGATGAC
    TCGGGCCCAC CCACAAGGGA AAGTTCTTCT AGACAGGAAA ACTTGAAAAC CGAGGCTCCT
    GTCTCCCCTG GTGCTGCTCC TTGGAGGCCT GGCATGTCAA ATGATGAGGC AGGGGGACAA
    CCTGAACCCG ACTCCAGAGA AGAGGTCCCG TCCATTAAGT CCCAGGTCAG AGAGAAGTGG
    GAGAAAGCGG CCCCCCTCAT CCCAGCATCG CCTGTGGGCT TGACAGCGGA GGAGGGTCTG
    GATCCCCCCG TCCACTTGGG TTCACTCTGG ACAGTGCACT CTGGCTGTGT TGACAGCAGC
    GGTGACTGCA GACAGCTGGA AGGTGACAAG CCCAGAATCA ATGGAGACTC GGAAGCTCTG
    AGTCCTCACA GCGAGTCCAC AGACACTGCC TCTGACTTCG AAGGCCACTT CTCCGAGGAC
    AGCAGTGAGG CCGAGCCTGG TGAAACCTTG GGGCCGAAGA GGTCCTTGGT GGCTGAGCAG
    GGTGAGAAAC ACAGTTGGAA CCACTGTGCC TCGCTCTCCA AGGTGAATGG TGACCTGAGT
    CTGGTCACCA GGACAGATGG GATGGTTGCT CCTCAGAGCT GGGTGTCTCG GGTATGTGCA
    GTTCCCCCAA AGATCCCAGA CTCCCTGCTG CTGGCCAGTC CCGAGTACCA GCCGAGACCC
    ATGTCCCTGG GCAGGCCTGC ATCCTCAGTG GAGGCTGCTA ACCCCCTTGT GATGCAGTTG
    CTGCAGGGCC ACTTGCCCCT CAAGAAGGTT CTCCCTCCAG CCCATGGCGG CAGCAAACCC
    GAACCCCCAC GACTCGCACT TACAGCAGAG CAGAGCCCTG GTGGCTCCCT GGGGATGGGG
    TCATTGCAAG GTCCTGGGGA GAACAGATGT GAAGTTGGCA AGAGCAGTCC AGAGTCTCTG
    CTACCTGAGA GCTGTGAAGC AAGTACCGGC TTTGCCCGGC TGGAGGCTAG CCAGGCTCCT
    GGGGCGCCCC TAAAGATGTC CAAGAACGCC CCGAGTTTAG ACTCCCTATA TCCAGTGACC
    AATCCAGTGG CTGCCTCTGG GAAAGCAGAA GTGGATTTCA AAGAACAGTT ATCTCCCTTC
    AGTTTTGAAG ATCAGAAGGA AGCCCGTAAC CTGTCCCAGT GCAGTATTTC AACTGCTGCC
    CCAGGTGTGA GTCTGGGAAA TCTCACTACC TCGAGAGCCC CTCTTTTCTC ATCTCCAAAT
    GCGGTCTCCT CGGGTCCTGA TCAGGCAGGT CGGGCCCTGG GGGATCAGAA CAGTGCGGGA
    GGCCAAGGGA AGAAGCTCTT TGGCTCCAAG AACGCAGCTG CAGCCCTTCA GTGCCCCCGG
    CTGGTGGAGC CGACGCCACT GCCTGCGGAG GCCCCTCCTG CTTTTCCCAA TAGGAAGTCA
    GGGCCAGGCA AAACCTCTCT GTCTGGTGGG ATACAGACTG CCAGGGAAGA CTGGGCCCCA
    AAGCCACCGC CTGCCTCTGT TGGCAGCATC AAGAGCGAAA AGACTCTTGA TGGAGGGCCT
    CTCAAGGTGG ATGCAGAGAA CAGAAAGGCA GTGGGGCCTG GTCCCCGGGA GCTGGTGGAT
    CACTTGCAAG GGATGCCCTT TGTCCTTGAT TTGCCCTTCT GGAAATTACC CCGGGAGCCC
    GGGAAGGGAC TCAGTCAGCC TCTGGAGCCT TCTTCCATCC CCTCCCAACT CAACATCAAG
    CAGGCATTTT ATGGGAAGCT TTCCAAACTC CAGCTAAGTT CCACCAGCTT TAATTATTCC
    TCCGGCTCCC CCCCTTTTCC CAAAGGCCTG GCCGGAAGTG TGGTGCAGCT GAGCCACAAA
    GCAAACTTTG GTGCGAGCCA CAGTGCGTCC CTCTCCTTGC AAATGTTCAC CGACAGCAGC
    ACAGTGGAAA GCATCTCGCT CCAGTGTGCG TGCAGCCTGA AAGCCATGAT CATGTGCCAG
    GGCTGCGGTG CATTCTGTCA CGATGACTGT ATTGGACCCT CAAAGCTCTG TGTATTGTGC
    CTTGTGGTGA GATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92396
    Clone ID Related Accession (Same CDS sequence) XM_015474264.1 , XM_015466141.1
    Accession Version XM_015474264.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4452bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product putative Polycomb group protein ASXL1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104370.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGACTCTGG GCACCAAAAG GAAATTTGCT TGCCAGAGTT TTTACTTGTT AGAGGTACTG 
    GAAAACTATT CAGATGCTCC AATGACACCC AAACAGATTC TGCAGGTCAT AGAGGCAGAA
    GGACTAAAGG AAATGAGAAG TGGGACATCC CCTCTCGCAT GTCTTAATGC CATGCTACAT
    TCCAATTCCA GAGGAGGAGA GGGGTTATTT TATAAACTAC CTGGCCGAAT CAGCCTTTTT
    ACACTCAAGA AGGATGCCCT GCAGTGGTCT CGCAGTCCAG CTGTGGTGGA GGGAGAGGAG
    CCAGAGGACA CAGCTGATGT GGAGAGCTGT GGGTCTAATG AAGCCAGCAC TGTGAGTGGT
    GAAAATGATG TTTCTCTCGA TGAAACATCT TCTAATGCTT CCTGTTCTAC GGAATCTCAG
    AGTCGGCCTC TTTCCAACCC CAGGGACAGC TGCAGAGCTT CCTCACAGGC AAACAAGCAG
    AAGAAAAAGA CAGGGGTGAT GCTGCCTCGA GTTGTCCTGA CTCCTCTGAA GGTAAACGGG
    GCCCACGTGG AATCAGCATC AGGTCAAATG AAGCGCAACA GAGGGGAAGA GATCGATTTT
    GAGACGCCTG GGTCCATTCT CGTCAACACC AACCTCCGGG CCCTGATCAA CTCGCGGACC
    TTCCATGCCC TGCCGTCCCA CTTCCAGCAG CAGCTCCTCT TCCTCCTGCC CGAAGTAGAC
    AGACAGGTGG GAACTGATGG CCTGTTACGT CTCAGCAGTA GTGCGCTGAA CAATGAGTTT
    TTCACCCACG CAGCTCAGAG CTGGCGGGAA CGCCTGGCTG ATGGTGAATT CACTCATGAG
    ATGCAGGTCA GGATACGACA AGAAATGGAG AAGGAAAAGA AAGTGGAACA ATGGAAAGAA
    AAGTTTTTCG AAGACTACTA TGGACAGAAG CTGGGTTTAA CCAAAGAAGA GTCATTGCAG
    CAGAATGTGG GCCAGGAGGA GGCTGAAATC AAGAGTAACC TGCGTGTCTC AGAATCAACA
    CGGCCACAGC GTGGCCCGGC CACCCGGCAG CGAGATGGGC ATTTCAAGAA ACGCTCTCGG
    CCAGATCTTC GAACCAGAGC CCGAAGGAAT CTGTACAAAA GACAGGAGCC AGAACAAGCA
    GAGATCGCTA AAGACGCACA GTCTGTGACA CTAGACATCC CCCTCCACAA GGACGGGGAG
    GCTAAGACCG ACGCAGCAGG GGTGGGCAGC CCCCACCCAC CTGGCACGTC CTCCGCAGCA
    TCCAACCCAG AGAGTCCCGA ATTCCCAGTG GAAACTGTGG CCTCCCGGCT CCAGCCCAGT
    CCCAATGACC AGGCACGTGT CTCCGCGTCT CCAGACAGAA TTCCCAGCTT GCTGCAGGAG
    ACCGTGGATC AGGAACCCAA GGATCAGAAG AGGAAATCCT TTGAGCAGGC GACCTCTGCC
    TCCTTTCCCG AAAAGAAGCC CCGGCTTGAA GATCGTCAGT CCTTTCGTAA CACAATTGAA
    AGTGTTCACC CCGAAAAGCC ACAGCCCACC AAAGAGGAGC CCAAAGTCCC GCCCATCCGG
    ATTCAACTTT CACGTATCAA ACCACCCTGG GTGGTTAAAG GTCAGCCCAC TTACCAGATA
    TGTCCCCGCA TCGTCCCCAT CACGGAGTCC TCCTGCCGGG GCTGGACTGG TGCCAGGACC
    CTTGCAGACA TTAAAGCCCG TGCTCTGCAG GTCCGAGGGG CGAGAGGCCA CCACTGCCAT
    CGAGAGGCGG CCACCACTGC CATCGGAGGT GGGGGTGGCC CGGGTGGAGG TGGCGGCGGG
    GCCACCGATG AGGGAGGTGG CAGAGGCGGC GGCAGTGGTG ATGGTGGTGA GGCCTGTGGC
    CACCCTGAGC CCCGGGGGGC CCCGAGTGCC CCTGGAGAGT GTGCGTCAGA TCTACAGCGA
    ACACAACTAC TGCCGCCTTG TCCTCTAAAC GGGGATCACG CCCAGGCCGA AACTGCTGCG
    TCCAGAGCCA GGAGAGAGGA CCTGGCTTCT CTCAGAAAGG GGCAGAGCTG CCCACTGCAG
    AGGGTCCCAG ATGGCCCTAG CGGCGGGCTG GAAGATGCCT CCCGACTCCC CGTTGCATCC
    ACTCGGGATC CGCCATGCCA GGCTCTGCCC CCACTGCCCT CTAGAATCCC AGAACCTGAG
    AGGTTAGTTG AGCAGCTTGT GTCGCATCCA GAAGGTAGAA CTGAATGTGG GTCTGGTACC
    ACTGCCTGGG AAAGGGGTGA TGAGAAGCTA GCACCTACCA TCCCCTCAGA GAACAGTCCT
    GTTCGGGCTC TGGCCGGGCC TACTGGATTA GAAGAAGGAA CTGGCCAGGC TCCAGACAGT
    GGCAGTAATC CCACCATGAA GGATCCTGTG AACGTGACCC CCAGTTCCAT CTCCGAATCA
    TCCTTGGCCA GTTGCCTGCA AGACAGACCA TTTGATGATG AATCAGAACT TGATGACTCG
    GGCCCACCCA CAAGGGAAAG TTCTTCTAGA CAGGAAAACT TGAAAACCGA GGCTCCTGTC
    TCCCCTGGTG CTGCTCCTTG GAGGCCTGGC ATGTCAAATG ATGAGGCAGG GGGACAACCT
    GAACCCGACT CCAGAGAAGA GGTCCCGTCC ATTAAGTCCC AGGTCAGAGA GAAGTGGGAG
    AAAGCGGCCC CCCTCATCCC AGCATCGCCT GTGGGCTTGA CAGCGGAGGA GGGTCTGGAT
    CCCCCCGTCC ACTTGGGTTC ACTCTGGACA GTGCACTCTG GCTGTGTTGA CAGCAGCGGT
    GACTGCAGAC AGCTGGAAGG TGACAAGCCC AGAATCAATG GAGACTCGGA AGCTCTGAGT
    CCTCACAGCG AGTCCACAGA CACTGCCTCT GACTTCGAAG GCCACTTCTC CGAGGACAGC
    AGTGAGGCCG AGCCTGGTGA AACCTTGGGG CCGAAGAGGT CCTTGGTGGC TGAGCAGGGT
    GAGAAACACA GTTGGAACCA CTGTGCCTCG CTCTCCAAGG TGAATGGTGA CCTGAGTCTG
    GTCACCAGGA CAGATGGGAT GGTTGCTCCT CAGAGCTGGG TGTCTCGGGT ATGTGCAGTT
    CCCCCAAAGA TCCCAGACTC CCTGCTGCTG GCCAGTCCCG AGTACCAGCC GAGACCCATG
    TCCCTGGGCA GGCCTGCATC CTCAGTGGAG GCTGCTAACC CCCTTGTGAT GCAGTTGCTG
    CAGGGCCACT TGCCCCTCAA GAAGGTTCTC CCTCCAGCCC ATGGCGGCAG CAAACCCGAA
    CCCCCACGAC TCGCACTTAC AGCAGAGCAG AGCCCTGGTG GCTCCCTGGG GATGGGGTCA
    TTGCAAGGTC CTGGGGAGAA CAGATGTGAA GTTGGCAAGA GCAGTCCAGA GTCTCTGCTA
    CCTGAGAGCT GTGAAGCAAG TACCGGCTTT GCCCGGCTGG AGGCTAGCCA GGCTCCTGGG
    GCGCCCCTAA AGATGTCCAA GAACGCCCCG AGTTTAGACT CCCTATATCC AGTGACCAAT
    CCAGTGGCTG CCTCTGGGAA AGCAGAAGTG GATTTCAAAG AACAGTTATC TCCCTTCAGT
    TTTGAAGATC AGAAGGAAGC CCGTAACCTG TCCCAGTGCA GTATTTCAAC TGCTGCCCCA
    GGTGTGAGTC TGGGAAATCT CACTACCTCG AGAGCCCCTC TTTTCTCATC TCCAAATGCG
    GTCTCCTCGG GTCCTGATCA GGCAGGTCGG GCCCTGGGGG ATCAGAACAG TGCGGGAGGC
    CAAGGGAAGA AGCTCTTTGG CTCCAAGAAC GCAGCTGCAG CCCTTCAGTG CCCCCGGCTG
    GTGGAGCCGA CGCCACTGCC TGCGGAGGCC CCTCCTGCTT TTCCCAATAG GAAGTCAGGG
    CCAGGCAAAA CCTCTCTGTC TGGTGGGATA CAGACTGCCA GGGAAGACTG GGCCCCAAAG
    CCACCGCCTG CCTCTGTTGG CAGCATCAAG AGCGAAAAGA CTCTTGATGG AGGGCCTCTC
    AAGGTGGATG CAGAGAACAG AAAGGCAGTG GGGCCTGGTC CCCGGGAGCT GGTGGATCAC
    TTGCAAGGGA TGCCCTTTGT CCTTGATTTG CCCTTCTGGA AATTACCCCG GGAGCCCGGG
    AAGGGACTCA GTCAGCCTCT GGAGCCTTCT TCCATCCCCT CCCAACTCAA CATCAAGCAG
    GCATTTTATG GGAAGCTTTC CAAACTCCAG CTAAGTTCCA CCAGCTTTAA TTATTCCTCC
    GGCTCCCCCC CTTTTCCCAA AGGCCTGGCC GGAAGTGTGG TGCAGCTGAG CCACAAAGCA
    AACTTTGGTG CGAGCCACAG TGCGTCCCTC TCCTTGCAAA TGTTCACCGA CAGCAGCACA
    GTGGAAAGCA TCTCGCTCCA GTGTGCGTGC AGCCTGAAAG CCATGATCAT GTGCCAGGGC
    TGCGGTGCAT TCTGTCACGA TGACTGTATT GGACCCTCAA AGCTCTGTGT ATTGTGCCTT
    GTGGTGAGAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329750.1
    CDS455..4906
    Translation

    Target ORF information:

    RefSeq Version XM_015474264.1
    Organism Bos taurus(cattle)
    Definition Bos taurus additional sex combs like 1, transcriptional regulator (ASXL1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474264.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGACTCTGG GCACCAAAAG GAAATTTGCT TGCCAGAGTT TTTACTTGTT AGAGGTACTG 
    GAAAACTATT CAGATGCTCC AATGACACCC AAACAGATTC TGCAGGTCAT AGAGGCAGAA
    GGACTAAAGG AAATGAGAAG TGGGACATCC CCTCTCGCAT GTCTTAATGC CATGCTACAT
    TCCAATTCCA GAGGAGGAGA GGGGTTATTT TATAAACTAC CTGGCCGAAT CAGCCTTTTT
    ACACTCAAGA AGGATGCCCT GCAGTGGTCT CGCAGTCCAG CTGTGGTGGA GGGAGAGGAG
    CCAGAGGACA CAGCTGATGT GGAGAGCTGT GGGTCTAATG AAGCCAGCAC TGTGAGTGGT
    GAAAATGATG TTTCTCTCGA TGAAACATCT TCTAATGCTT CCTGTTCTAC GGAATCTCAG
    AGTCGGCCTC TTTCCAACCC CAGGGACAGC TGCAGAGCTT CCTCACAGGC AAACAAGCAG
    AAGAAAAAGA CAGGGGTGAT GCTGCCTCGA GTTGTCCTGA CTCCTCTGAA GGTAAACGGG
    GCCCACGTGG AATCAGCATC AGGTCAAATG AAGCGCAACA GAGGGGAAGA GATCGATTTT
    GAGACGCCTG GGTCCATTCT CGTCAACACC AACCTCCGGG CCCTGATCAA CTCGCGGACC
    TTCCATGCCC TGCCGTCCCA CTTCCAGCAG CAGCTCCTCT TCCTCCTGCC CGAAGTAGAC
    AGACAGGTGG GAACTGATGG CCTGTTACGT CTCAGCAGTA GTGCGCTGAA CAATGAGTTT
    TTCACCCACG CAGCTCAGAG CTGGCGGGAA CGCCTGGCTG ATGGTGAATT CACTCATGAG
    ATGCAGGTCA GGATACGACA AGAAATGGAG AAGGAAAAGA AAGTGGAACA ATGGAAAGAA
    AAGTTTTTCG AAGACTACTA TGGACAGAAG CTGGGTTTAA CCAAAGAAGA GTCATTGCAG
    CAGAATGTGG GCCAGGAGGA GGCTGAAATC AAGAGTAACC TGCGTGTCTC AGAATCAACA
    CGGCCACAGC GTGGCCCGGC CACCCGGCAG CGAGATGGGC ATTTCAAGAA ACGCTCTCGG
    CCAGATCTTC GAACCAGAGC CCGAAGGAAT CTGTACAAAA GACAGGAGCC AGAACAAGCA
    GAGATCGCTA AAGACGCACA GTCTGTGACA CTAGACATCC CCCTCCACAA GGACGGGGAG
    GCTAAGACCG ACGCAGCAGG GGTGGGCAGC CCCCACCCAC CTGGCACGTC CTCCGCAGCA
    TCCAACCCAG AGAGTCCCGA ATTCCCAGTG GAAACTGTGG CCTCCCGGCT CCAGCCCAGT
    CCCAATGACC AGGCACGTGT CTCCGCGTCT CCAGACAGAA TTCCCAGCTT GCTGCAGGAG
    ACCGTGGATC AGGAACCCAA GGATCAGAAG AGGAAATCCT TTGAGCAGGC GACCTCTGCC
    TCCTTTCCCG AAAAGAAGCC CCGGCTTGAA GATCGTCAGT CCTTTCGTAA CACAATTGAA
    AGTGTTCACC CCGAAAAGCC ACAGCCCACC AAAGAGGAGC CCAAAGTCCC GCCCATCCGG
    ATTCAACTTT CACGTATCAA ACCACCCTGG GTGGTTAAAG GTCAGCCCAC TTACCAGATA
    TGTCCCCGCA TCGTCCCCAT CACGGAGTCC TCCTGCCGGG GCTGGACTGG TGCCAGGACC
    CTTGCAGACA TTAAAGCCCG TGCTCTGCAG GTCCGAGGGG CGAGAGGCCA CCACTGCCAT
    CGAGAGGCGG CCACCACTGC CATCGGAGGT GGGGGTGGCC CGGGTGGAGG TGGCGGCGGG
    GCCACCGATG AGGGAGGTGG CAGAGGCGGC GGCAGTGGTG ATGGTGGTGA GGCCTGTGGC
    CACCCTGAGC CCCGGGGGGC CCCGAGTGCC CCTGGAGAGT GTGCGTCAGA TCTACAGCGA
    ACACAACTAC TGCCGCCTTG TCCTCTAAAC GGGGATCACG CCCAGGCCGA AACTGCTGCG
    TCCAGAGCCA GGAGAGAGGA CCTGGCTTCT CTCAGAAAGG GGCAGAGCTG CCCACTGCAG
    AGGGTCCCAG ATGGCCCTAG CGGCGGGCTG GAAGATGCCT CCCGACTCCC CGTTGCATCC
    ACTCGGGATC CGCCATGCCA GGCTCTGCCC CCACTGCCCT CTAGAATCCC AGAACCTGAG
    AGGTTAGTTG AGCAGCTTGT GTCGCATCCA GAAGGTAGAA CTGAATGTGG GTCTGGTACC
    ACTGCCTGGG AAAGGGGTGA TGAGAAGCTA GCACCTACCA TCCCCTCAGA GAACAGTCCT
    GTTCGGGCTC TGGCCGGGCC TACTGGATTA GAAGAAGGAA CTGGCCAGGC TCCAGACAGT
    GGCAGTAATC CCACCATGAA GGATCCTGTG AACGTGACCC CCAGTTCCAT CTCCGAATCA
    TCCTTGGCCA GTTGCCTGCA AGACAGACCA TTTGATGATG AATCAGAACT TGATGACTCG
    GGCCCACCCA CAAGGGAAAG TTCTTCTAGA CAGGAAAACT TGAAAACCGA GGCTCCTGTC
    TCCCCTGGTG CTGCTCCTTG GAGGCCTGGC ATGTCAAATG ATGAGGCAGG GGGACAACCT
    GAACCCGACT CCAGAGAAGA GGTCCCGTCC ATTAAGTCCC AGGTCAGAGA GAAGTGGGAG
    AAAGCGGCCC CCCTCATCCC AGCATCGCCT GTGGGCTTGA CAGCGGAGGA GGGTCTGGAT
    CCCCCCGTCC ACTTGGGTTC ACTCTGGACA GTGCACTCTG GCTGTGTTGA CAGCAGCGGT
    GACTGCAGAC AGCTGGAAGG TGACAAGCCC AGAATCAATG GAGACTCGGA AGCTCTGAGT
    CCTCACAGCG AGTCCACAGA CACTGCCTCT GACTTCGAAG GCCACTTCTC CGAGGACAGC
    AGTGAGGCCG AGCCTGGTGA AACCTTGGGG CCGAAGAGGT CCTTGGTGGC TGAGCAGGGT
    GAGAAACACA GTTGGAACCA CTGTGCCTCG CTCTCCAAGG TGAATGGTGA CCTGAGTCTG
    GTCACCAGGA CAGATGGGAT GGTTGCTCCT CAGAGCTGGG TGTCTCGGGT ATGTGCAGTT
    CCCCCAAAGA TCCCAGACTC CCTGCTGCTG GCCAGTCCCG AGTACCAGCC GAGACCCATG
    TCCCTGGGCA GGCCTGCATC CTCAGTGGAG GCTGCTAACC CCCTTGTGAT GCAGTTGCTG
    CAGGGCCACT TGCCCCTCAA GAAGGTTCTC CCTCCAGCCC ATGGCGGCAG CAAACCCGAA
    CCCCCACGAC TCGCACTTAC AGCAGAGCAG AGCCCTGGTG GCTCCCTGGG GATGGGGTCA
    TTGCAAGGTC CTGGGGAGAA CAGATGTGAA GTTGGCAAGA GCAGTCCAGA GTCTCTGCTA
    CCTGAGAGCT GTGAAGCAAG TACCGGCTTT GCCCGGCTGG AGGCTAGCCA GGCTCCTGGG
    GCGCCCCTAA AGATGTCCAA GAACGCCCCG AGTTTAGACT CCCTATATCC AGTGACCAAT
    CCAGTGGCTG CCTCTGGGAA AGCAGAAGTG GATTTCAAAG AACAGTTATC TCCCTTCAGT
    TTTGAAGATC AGAAGGAAGC CCGTAACCTG TCCCAGTGCA GTATTTCAAC TGCTGCCCCA
    GGTGTGAGTC TGGGAAATCT CACTACCTCG AGAGCCCCTC TTTTCTCATC TCCAAATGCG
    GTCTCCTCGG GTCCTGATCA GGCAGGTCGG GCCCTGGGGG ATCAGAACAG TGCGGGAGGC
    CAAGGGAAGA AGCTCTTTGG CTCCAAGAAC GCAGCTGCAG CCCTTCAGTG CCCCCGGCTG
    GTGGAGCCGA CGCCACTGCC TGCGGAGGCC CCTCCTGCTT TTCCCAATAG GAAGTCAGGG
    CCAGGCAAAA CCTCTCTGTC TGGTGGGATA CAGACTGCCA GGGAAGACTG GGCCCCAAAG
    CCACCGCCTG CCTCTGTTGG CAGCATCAAG AGCGAAAAGA CTCTTGATGG AGGGCCTCTC
    AAGGTGGATG CAGAGAACAG AAAGGCAGTG GGGCCTGGTC CCCGGGAGCT GGTGGATCAC
    TTGCAAGGGA TGCCCTTTGT CCTTGATTTG CCCTTCTGGA AATTACCCCG GGAGCCCGGG
    AAGGGACTCA GTCAGCCTCT GGAGCCTTCT TCCATCCCCT CCCAACTCAA CATCAAGCAG
    GCATTTTATG GGAAGCTTTC CAAACTCCAG CTAAGTTCCA CCAGCTTTAA TTATTCCTCC
    GGCTCCCCCC CTTTTCCCAA AGGCCTGGCC GGAAGTGTGG TGCAGCTGAG CCACAAAGCA
    AACTTTGGTG CGAGCCACAG TGCGTCCCTC TCCTTGCAAA TGTTCACCGA CAGCAGCACA
    GTGGAAAGCA TCTCGCTCCA GTGTGCGTGC AGCCTGAAAG CCATGATCAT GTGCCAGGGC
    TGCGGTGCAT TCTGTCACGA TGACTGTATT GGACCCTCAA AGCTCTGTGT ATTGTGCCTT
    GTGGTGAGAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92396
    Clone ID Related Accession (Same CDS sequence) XM_015474264.1 , XM_015466141.1
    Accession Version XM_015466141.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4452bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product putative Polycomb group protein ASXL1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646145.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGACTCTGG GCACCAAAAG GAAATTTGCT TGCCAGAGTT TTTACTTGTT AGAGGTACTG 
    GAAAACTATT CAGATGCTCC AATGACACCC AAACAGATTC TGCAGGTCAT AGAGGCAGAA
    GGACTAAAGG AAATGAGAAG TGGGACATCC CCTCTCGCAT GTCTTAATGC CATGCTACAT
    TCCAATTCCA GAGGAGGAGA GGGGTTATTT TATAAACTAC CTGGCCGAAT CAGCCTTTTT
    ACACTCAAGA AGGATGCCCT GCAGTGGTCT CGCAGTCCAG CTGTGGTGGA GGGAGAGGAG
    CCAGAGGACA CAGCTGATGT GGAGAGCTGT GGGTCTAATG AAGCCAGCAC TGTGAGTGGT
    GAAAATGATG TTTCTCTCGA TGAAACATCT TCTAATGCTT CCTGTTCTAC GGAATCTCAG
    AGTCGGCCTC TTTCCAACCC CAGGGACAGC TGCAGAGCTT CCTCACAGGC AAACAAGCAG
    AAGAAAAAGA CAGGGGTGAT GCTGCCTCGA GTTGTCCTGA CTCCTCTGAA GGTAAACGGG
    GCCCACGTGG AATCAGCATC AGGTCAAATG AAGCGCAACA GAGGGGAAGA GATCGATTTT
    GAGACGCCTG GGTCCATTCT CGTCAACACC AACCTCCGGG CCCTGATCAA CTCGCGGACC
    TTCCATGCCC TGCCGTCCCA CTTCCAGCAG CAGCTCCTCT TCCTCCTGCC CGAAGTAGAC
    AGACAGGTGG GAACTGATGG CCTGTTACGT CTCAGCAGTA GTGCGCTGAA CAATGAGTTT
    TTCACCCACG CAGCTCAGAG CTGGCGGGAA CGCCTGGCTG ATGGTGAATT CACTCATGAG
    ATGCAGGTCA GGATACGACA AGAAATGGAG AAGGAAAAGA AAGTGGAACA ATGGAAAGAA
    AAGTTTTTCG AAGACTACTA TGGACAGAAG CTGGGTTTAA CCAAAGAAGA GTCATTGCAG
    CAGAATGTGG GCCAGGAGGA GGCTGAAATC AAGAGTAACC TGCGTGTCTC AGAATCAACA
    CGGCCACAGC GTGGCCCGGC CACCCGGCAG CGAGATGGGC ATTTCAAGAA ACGCTCTCGG
    CCAGATCTTC GAACCAGAGC CCGAAGGAAT CTGTACAAAA GACAGGAGCC AGAACAAGCA
    GAGATCGCTA AAGACGCACA GTCTGTGACA CTAGACATCC CCCTCCACAA GGACGGGGAG
    GCTAAGACCG ACGCAGCAGG GGTGGGCAGC CCCCACCCAC CTGGCACGTC CTCCGCAGCA
    TCCAACCCAG AGAGTCCCGA ATTCCCAGTG GAAACTGTGG CCTCCCGGCT CCAGCCCAGT
    CCCAATGACC AGGCACGTGT CTCCGCGTCT CCAGACAGAA TTCCCAGCTT GCTGCAGGAG
    ACCGTGGATC AGGAACCCAA GGATCAGAAG AGGAAATCCT TTGAGCAGGC GACCTCTGCC
    TCCTTTCCCG AAAAGAAGCC CCGGCTTGAA GATCGTCAGT CCTTTCGTAA CACAATTGAA
    AGTGTTCACC CCGAAAAGCC ACAGCCCACC AAAGAGGAGC CCAAAGTCCC GCCCATCCGG
    ATTCAACTTT CACGTATCAA ACCACCCTGG GTGGTTAAAG GTCAGCCCAC TTACCAGATA
    TGTCCCCGCA TCGTCCCCAT CACGGAGTCC TCCTGCCGGG GCTGGACTGG TGCCAGGACC
    CTTGCAGACA TTAAAGCCCG TGCTCTGCAG GTCCGAGGGG CGAGAGGCCA CCACTGCCAT
    CGAGAGGCGG CCACCACTGC CATCGGAGGT GGGGGTGGCC CGGGTGGAGG TGGCGGCGGG
    GCCACCGATG AGGGAGGTGG CAGAGGCGGC GGCAGTGGTG ATGGTGGTGA GGCCTGTGGC
    CACCCTGAGC CCCGGGGGGC CCCGAGTGCC CCTGGAGAGT GTGCGTCAGA TCTACAGCGA
    ACACAACTAC TGCCGCCTTG TCCTCTAAAC GGGGATCACG CCCAGGCCGA AACTGCTGCG
    TCCAGAGCCA GGAGAGAGGA CCTGGCTTCT CTCAGAAAGG GGCAGAGCTG CCCACTGCAG
    AGGGTCCCAG ATGGCCCTAG CGGCGGGCTG GAAGATGCCT CCCGACTCCC CGTTGCATCC
    ACTCGGGATC CGCCATGCCA GGCTCTGCCC CCACTGCCCT CTAGAATCCC AGAACCTGAG
    AGGTTAGTTG AGCAGCTTGT GTCGCATCCA GAAGGTAGAA CTGAATGTGG GTCTGGTACC
    ACTGCCTGGG AAAGGGGTGA TGAGAAGCTA GCACCTACCA TCCCCTCAGA GAACAGTCCT
    GTTCGGGCTC TGGCCGGGCC TACTGGATTA GAAGAAGGAA CTGGCCAGGC TCCAGACAGT
    GGCAGTAATC CCACCATGAA GGATCCTGTG AACGTGACCC CCAGTTCCAT CTCCGAATCA
    TCCTTGGCCA GTTGCCTGCA AGACAGACCA TTTGATGATG AATCAGAACT TGATGACTCG
    GGCCCACCCA CAAGGGAAAG TTCTTCTAGA CAGGAAAACT TGAAAACCGA GGCTCCTGTC
    TCCCCTGGTG CTGCTCCTTG GAGGCCTGGC ATGTCAAATG ATGAGGCAGG GGGACAACCT
    GAACCCGACT CCAGAGAAGA GGTCCCGTCC ATTAAGTCCC AGGTCAGAGA GAAGTGGGAG
    AAAGCGGCCC CCCTCATCCC AGCATCGCCT GTGGGCTTGA CAGCGGAGGA GGGTCTGGAT
    CCCCCCGTCC ACTTGGGTTC ACTCTGGACA GTGCACTCTG GCTGTGTTGA CAGCAGCGGT
    GACTGCAGAC AGCTGGAAGG TGACAAGCCC AGAATCAATG GAGACTCGGA AGCTCTGAGT
    CCTCACAGCG AGTCCACAGA CACTGCCTCT GACTTCGAAG GCCACTTCTC CGAGGACAGC
    AGTGAGGCCG AGCCTGGTGA AACCTTGGGG CCGAAGAGGT CCTTGGTGGC TGAGCAGGGT
    GAGAAACACA GTTGGAACCA CTGTGCCTCG CTCTCCAAGG TGAATGGTGA CCTGAGTCTG
    GTCACCAGGA CAGATGGGAT GGTTGCTCCT CAGAGCTGGG TGTCTCGGGT ATGTGCAGTT
    CCCCCAAAGA TCCCAGACTC CCTGCTGCTG GCCAGTCCCG AGTACCAGCC GAGACCCATG
    TCCCTGGGCA GGCCTGCATC CTCAGTGGAG GCTGCTAACC CCCTTGTGAT GCAGTTGCTG
    CAGGGCCACT TGCCCCTCAA GAAGGTTCTC CCTCCAGCCC ATGGCGGCAG CAAACCCGAA
    CCCCCACGAC TCGCACTTAC AGCAGAGCAG AGCCCTGGTG GCTCCCTGGG GATGGGGTCA
    TTGCAAGGTC CTGGGGAGAA CAGATGTGAA GTTGGCAAGA GCAGTCCAGA GTCTCTGCTA
    CCTGAGAGCT GTGAAGCAAG TACCGGCTTT GCCCGGCTGG AGGCTAGCCA GGCTCCTGGG
    GCGCCCCTAA AGATGTCCAA GAACGCCCCG AGTTTAGACT CCCTATATCC AGTGACCAAT
    CCAGTGGCTG CCTCTGGGAA AGCAGAAGTG GATTTCAAAG AACAGTTATC TCCCTTCAGT
    TTTGAAGATC AGAAGGAAGC CCGTAACCTG TCCCAGTGCA GTATTTCAAC TGCTGCCCCA
    GGTGTGAGTC TGGGAAATCT CACTACCTCG AGAGCCCCTC TTTTCTCATC TCCAAATGCG
    GTCTCCTCGG GTCCTGATCA GGCAGGTCGG GCCCTGGGGG ATCAGAACAG TGCGGGAGGC
    CAAGGGAAGA AGCTCTTTGG CTCCAAGAAC GCAGCTGCAG CCCTTCAGTG CCCCCGGCTG
    GTGGAGCCGA CGCCACTGCC TGCGGAGGCC CCTCCTGCTT TTCCCAATAG GAAGTCAGGG
    CCAGGCAAAA CCTCTCTGTC TGGTGGGATA CAGACTGCCA GGGAAGACTG GGCCCCAAAG
    CCACCGCCTG CCTCTGTTGG CAGCATCAAG AGCGAAAAGA CTCTTGATGG AGGGCCTCTC
    AAGGTGGATG CAGAGAACAG AAAGGCAGTG GGGCCTGGTC CCCGGGAGCT GGTGGATCAC
    TTGCAAGGGA TGCCCTTTGT CCTTGATTTG CCCTTCTGGA AATTACCCCG GGAGCCCGGG
    AAGGGACTCA GTCAGCCTCT GGAGCCTTCT TCCATCCCCT CCCAACTCAA CATCAAGCAG
    GCATTTTATG GGAAGCTTTC CAAACTCCAG CTAAGTTCCA CCAGCTTTAA TTATTCCTCC
    GGCTCCCCCC CTTTTCCCAA AGGCCTGGCC GGAAGTGTGG TGCAGCTGAG CCACAAAGCA
    AACTTTGGTG CGAGCCACAG TGCGTCCCTC TCCTTGCAAA TGTTCACCGA CAGCAGCACA
    GTGGAAAGCA TCTCGCTCCA GTGTGCGTGC AGCCTGAAAG CCATGATCAT GTGCCAGGGC
    TGCGGTGCAT TCTGTCACGA TGACTGTATT GGACCCTCAA AGCTCTGTGT ATTGTGCCTT
    GTGGTGAGAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015321627.1
    CDS477..4928
    Translation

    Target ORF information:

    RefSeq Version XM_015466141.1
    Organism Bos taurus(cattle)
    Definition Bos taurus additional sex combs like 1, transcriptional regulator (ASXL1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015466141.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGACTCTGG GCACCAAAAG GAAATTTGCT TGCCAGAGTT TTTACTTGTT AGAGGTACTG 
    GAAAACTATT CAGATGCTCC AATGACACCC AAACAGATTC TGCAGGTCAT AGAGGCAGAA
    GGACTAAAGG AAATGAGAAG TGGGACATCC CCTCTCGCAT GTCTTAATGC CATGCTACAT
    TCCAATTCCA GAGGAGGAGA GGGGTTATTT TATAAACTAC CTGGCCGAAT CAGCCTTTTT
    ACACTCAAGA AGGATGCCCT GCAGTGGTCT CGCAGTCCAG CTGTGGTGGA GGGAGAGGAG
    CCAGAGGACA CAGCTGATGT GGAGAGCTGT GGGTCTAATG AAGCCAGCAC TGTGAGTGGT
    GAAAATGATG TTTCTCTCGA TGAAACATCT TCTAATGCTT CCTGTTCTAC GGAATCTCAG
    AGTCGGCCTC TTTCCAACCC CAGGGACAGC TGCAGAGCTT CCTCACAGGC AAACAAGCAG
    AAGAAAAAGA CAGGGGTGAT GCTGCCTCGA GTTGTCCTGA CTCCTCTGAA GGTAAACGGG
    GCCCACGTGG AATCAGCATC AGGTCAAATG AAGCGCAACA GAGGGGAAGA GATCGATTTT
    GAGACGCCTG GGTCCATTCT CGTCAACACC AACCTCCGGG CCCTGATCAA CTCGCGGACC
    TTCCATGCCC TGCCGTCCCA CTTCCAGCAG CAGCTCCTCT TCCTCCTGCC CGAAGTAGAC
    AGACAGGTGG GAACTGATGG CCTGTTACGT CTCAGCAGTA GTGCGCTGAA CAATGAGTTT
    TTCACCCACG CAGCTCAGAG CTGGCGGGAA CGCCTGGCTG ATGGTGAATT CACTCATGAG
    ATGCAGGTCA GGATACGACA AGAAATGGAG AAGGAAAAGA AAGTGGAACA ATGGAAAGAA
    AAGTTTTTCG AAGACTACTA TGGACAGAAG CTGGGTTTAA CCAAAGAAGA GTCATTGCAG
    CAGAATGTGG GCCAGGAGGA GGCTGAAATC AAGAGTAACC TGCGTGTCTC AGAATCAACA
    CGGCCACAGC GTGGCCCGGC CACCCGGCAG CGAGATGGGC ATTTCAAGAA ACGCTCTCGG
    CCAGATCTTC GAACCAGAGC CCGAAGGAAT CTGTACAAAA GACAGGAGCC AGAACAAGCA
    GAGATCGCTA AAGACGCACA GTCTGTGACA CTAGACATCC CCCTCCACAA GGACGGGGAG
    GCTAAGACCG ACGCAGCAGG GGTGGGCAGC CCCCACCCAC CTGGCACGTC CTCCGCAGCA
    TCCAACCCAG AGAGTCCCGA ATTCCCAGTG GAAACTGTGG CCTCCCGGCT CCAGCCCAGT
    CCCAATGACC AGGCACGTGT CTCCGCGTCT CCAGACAGAA TTCCCAGCTT GCTGCAGGAG
    ACCGTGGATC AGGAACCCAA GGATCAGAAG AGGAAATCCT TTGAGCAGGC GACCTCTGCC
    TCCTTTCCCG AAAAGAAGCC CCGGCTTGAA GATCGTCAGT CCTTTCGTAA CACAATTGAA
    AGTGTTCACC CCGAAAAGCC ACAGCCCACC AAAGAGGAGC CCAAAGTCCC GCCCATCCGG
    ATTCAACTTT CACGTATCAA ACCACCCTGG GTGGTTAAAG GTCAGCCCAC TTACCAGATA
    TGTCCCCGCA TCGTCCCCAT CACGGAGTCC TCCTGCCGGG GCTGGACTGG TGCCAGGACC
    CTTGCAGACA TTAAAGCCCG TGCTCTGCAG GTCCGAGGGG CGAGAGGCCA CCACTGCCAT
    CGAGAGGCGG CCACCACTGC CATCGGAGGT GGGGGTGGCC CGGGTGGAGG TGGCGGCGGG
    GCCACCGATG AGGGAGGTGG CAGAGGCGGC GGCAGTGGTG ATGGTGGTGA GGCCTGTGGC
    CACCCTGAGC CCCGGGGGGC CCCGAGTGCC CCTGGAGAGT GTGCGTCAGA TCTACAGCGA
    ACACAACTAC TGCCGCCTTG TCCTCTAAAC GGGGATCACG CCCAGGCCGA AACTGCTGCG
    TCCAGAGCCA GGAGAGAGGA CCTGGCTTCT CTCAGAAAGG GGCAGAGCTG CCCACTGCAG
    AGGGTCCCAG ATGGCCCTAG CGGCGGGCTG GAAGATGCCT CCCGACTCCC CGTTGCATCC
    ACTCGGGATC CGCCATGCCA GGCTCTGCCC CCACTGCCCT CTAGAATCCC AGAACCTGAG
    AGGTTAGTTG AGCAGCTTGT GTCGCATCCA GAAGGTAGAA CTGAATGTGG GTCTGGTACC
    ACTGCCTGGG AAAGGGGTGA TGAGAAGCTA GCACCTACCA TCCCCTCAGA GAACAGTCCT
    GTTCGGGCTC TGGCCGGGCC TACTGGATTA GAAGAAGGAA CTGGCCAGGC TCCAGACAGT
    GGCAGTAATC CCACCATGAA GGATCCTGTG AACGTGACCC CCAGTTCCAT CTCCGAATCA
    TCCTTGGCCA GTTGCCTGCA AGACAGACCA TTTGATGATG AATCAGAACT TGATGACTCG
    GGCCCACCCA CAAGGGAAAG TTCTTCTAGA CAGGAAAACT TGAAAACCGA GGCTCCTGTC
    TCCCCTGGTG CTGCTCCTTG GAGGCCTGGC ATGTCAAATG ATGAGGCAGG GGGACAACCT
    GAACCCGACT CCAGAGAAGA GGTCCCGTCC ATTAAGTCCC AGGTCAGAGA GAAGTGGGAG
    AAAGCGGCCC CCCTCATCCC AGCATCGCCT GTGGGCTTGA CAGCGGAGGA GGGTCTGGAT
    CCCCCCGTCC ACTTGGGTTC ACTCTGGACA GTGCACTCTG GCTGTGTTGA CAGCAGCGGT
    GACTGCAGAC AGCTGGAAGG TGACAAGCCC AGAATCAATG GAGACTCGGA AGCTCTGAGT
    CCTCACAGCG AGTCCACAGA CACTGCCTCT GACTTCGAAG GCCACTTCTC CGAGGACAGC
    AGTGAGGCCG AGCCTGGTGA AACCTTGGGG CCGAAGAGGT CCTTGGTGGC TGAGCAGGGT
    GAGAAACACA GTTGGAACCA CTGTGCCTCG CTCTCCAAGG TGAATGGTGA CCTGAGTCTG
    GTCACCAGGA CAGATGGGAT GGTTGCTCCT CAGAGCTGGG TGTCTCGGGT ATGTGCAGTT
    CCCCCAAAGA TCCCAGACTC CCTGCTGCTG GCCAGTCCCG AGTACCAGCC GAGACCCATG
    TCCCTGGGCA GGCCTGCATC CTCAGTGGAG GCTGCTAACC CCCTTGTGAT GCAGTTGCTG
    CAGGGCCACT TGCCCCTCAA GAAGGTTCTC CCTCCAGCCC ATGGCGGCAG CAAACCCGAA
    CCCCCACGAC TCGCACTTAC AGCAGAGCAG AGCCCTGGTG GCTCCCTGGG GATGGGGTCA
    TTGCAAGGTC CTGGGGAGAA CAGATGTGAA GTTGGCAAGA GCAGTCCAGA GTCTCTGCTA
    CCTGAGAGCT GTGAAGCAAG TACCGGCTTT GCCCGGCTGG AGGCTAGCCA GGCTCCTGGG
    GCGCCCCTAA AGATGTCCAA GAACGCCCCG AGTTTAGACT CCCTATATCC AGTGACCAAT
    CCAGTGGCTG CCTCTGGGAA AGCAGAAGTG GATTTCAAAG AACAGTTATC TCCCTTCAGT
    TTTGAAGATC AGAAGGAAGC CCGTAACCTG TCCCAGTGCA GTATTTCAAC TGCTGCCCCA
    GGTGTGAGTC TGGGAAATCT CACTACCTCG AGAGCCCCTC TTTTCTCATC TCCAAATGCG
    GTCTCCTCGG GTCCTGATCA GGCAGGTCGG GCCCTGGGGG ATCAGAACAG TGCGGGAGGC
    CAAGGGAAGA AGCTCTTTGG CTCCAAGAAC GCAGCTGCAG CCCTTCAGTG CCCCCGGCTG
    GTGGAGCCGA CGCCACTGCC TGCGGAGGCC CCTCCTGCTT TTCCCAATAG GAAGTCAGGG
    CCAGGCAAAA CCTCTCTGTC TGGTGGGATA CAGACTGCCA GGGAAGACTG GGCCCCAAAG
    CCACCGCCTG CCTCTGTTGG CAGCATCAAG AGCGAAAAGA CTCTTGATGG AGGGCCTCTC
    AAGGTGGATG CAGAGAACAG AAAGGCAGTG GGGCCTGGTC CCCGGGAGCT GGTGGATCAC
    TTGCAAGGGA TGCCCTTTGT CCTTGATTTG CCCTTCTGGA AATTACCCCG GGAGCCCGGG
    AAGGGACTCA GTCAGCCTCT GGAGCCTTCT TCCATCCCCT CCCAACTCAA CATCAAGCAG
    GCATTTTATG GGAAGCTTTC CAAACTCCAG CTAAGTTCCA CCAGCTTTAA TTATTCCTCC
    GGCTCCCCCC CTTTTCCCAA AGGCCTGGCC GGAAGTGTGG TGCAGCTGAG CCACAAAGCA
    AACTTTGGTG CGAGCCACAG TGCGTCCCTC TCCTTGCAAA TGTTCACCGA CAGCAGCACA
    GTGGAAAGCA TCTCGCTCCA GTGTGCGTGC AGCCTGAAAG CCATGATCAT GTGCCAGGGC
    TGCGGTGCAT TCTGTCACGA TGACTGTATT GGACCCTCAA AGCTCTGTGT ATTGTGCCTT
    GTGGTGAGAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222035
    Clone ID Related Accession (Same CDS sequence) XM_025001184.1
    Accession Version XM_025001184.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4608bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product putative Polycomb group protein ASXL1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAAGGACA AACAGAAGAG GAAGAAGGAG CGCACGTGGG CCGAGGCCGC GCGCCTGGTA 
    CTGGAAAACT ATTCAGATGC TCCAATGACA CCCAAACAGA TTCTGCAGGT CATAGAGGCA
    GAAGGACTAA AGGAAATGAG AAGTGGGACA TCCCCTCTCG CATGTCTTAA TGCCATGCTA
    CATTCCAATT CCAGAGGAGG AGAGGGGTTA TTTTATAAAC TACCTGGCCG AATCAGCCTT
    TTTACACTCA AGAAGGATGC CCTGCAGTGG TCTCGCAGTC CAGCTGTGGT GGAGGGAGAG
    GAGCCAGAGG ACACAGCTGA TGTGGAGAGC TGTGGGTCTA ATGAAGCCAG CACTGTGAGT
    GGTGAAAATG ATGTTTCTCT CGATGAAACA TCTTCTAATG CTTCCTGTTC TACGGAATCT
    CAGAGTCGGC CTCTTTCCAA CCCCAGGGAC AGCTGCAGAG CTTCCTCACA GGCAAACAAG
    CAGAAGAAAA AGACAGGGGT GATGCTGCCT CGAGTTGTCC TGACTCCTCT GAAGGTAAAC
    GGGGCCCACG TGGAATCAGC ATCAGGGTTC TCGGGCCGCC ACGCCGATGG CGAGAGCGGC
    AGCCCGTCCA GCAGCAGCGG CGGCTCTCCG GCCCTGGGCA GCGCTGCTAT CCGTGGCCAG
    GCCGAGCTCG CCCGGGACCC TGCCCCGCTC CTGAGAGGCT TCCGGAAGCC GGCCACAGGT
    CAAATGAAGC GCAACAGAGG GGAAGAGATC GATTTTGAGA CGCCTGGGTC CATTCTCGTC
    AACACCAACC TCCGGGCCCT GATCAACTCG CGGACCTTCC ATGCCCTGCC GTCCCACTTC
    CAGCAGCAGC TCCTCTTCCT CCTGCCCGAA GTAGACAGAC AGGTGGGAAC TGATGGCCTG
    TTACGTCTCA GCAGTAGTGC GCTGAACAAT GAGTTTTTCA CCCACGCAGC TCAGAGCTGG
    CGGGAACGCC TGGCTGATGG TGAATTCACT CATGAGATGC AGGTCAGGAT ACGACAAGAA
    ATGGAGAAGG AAAAGAAAGT GGAACAATGG AAAGAAAAGT TTTTCGAAGA CTACTATGGA
    CAGAAGCTGG GTTTAACCAA AGAAGAGTCA TTGCAGCAGA ATGTGGGCCA GGAGGAGGCT
    GAAATCAAGA GTAACCTGCG TGTCTCAGAA TCAACACGGC CACAGCGTGG CCCGGCCACC
    CGGCAGCGAG ATGGGCATTT CAAGAAACGC TCTCGGCCAG ATCTTCGAAC CAGAGCCCGA
    AGGAATCTGT ACAAAAGACA GGAGCCAGAA CAAGCAGAGA TCGCTAAAGA CGCACAGTCT
    GTGACACTAG ACATCCCCCT CCACAAGGAC GGGGAGGCTA AGACCGACGC AGCAGGGGTG
    GGCAGCCCCC ACCCACCTGG CACGTCCTCC GCAGCATCCA ACCCAGAGAG TCCCGAATTC
    CCAGTGGAAA CTGTGGCCTC CCGGCTCCAG CCCAGTCCCA ATGACCAGGC ACGTGTCTCC
    GCGTCTCCAG ACAGAATTCC CAGCTTGCTG CAGGAGACCG TGGATCAGGA ACCCAAGGAT
    CAGAAGAGGA AATCCTTTGA GCAGGCGACC TCTGCCTCCT TTCCCGAAAA GAAGCCCCGG
    CTTGAAGATC GTCAGTCCTT TCGTAACACA ATTGAAAGTG TTCACCCCGA AAAGCCACAG
    CCCACCAAAG AGGAGCCCAA AGTCCCGCCC ATCCGGATTC AACTTTCACG TATCAAACCA
    CCCTGGGTGG TTAAAGGTCA GCCCACTTAC CAGATATGTC CCCGCATCGT CCCCATCACG
    GAGTCCTCCT GCCGGGGCTG GACTGGTGCC AGGACCCTTG CAGACATTAA AGCCCGTGCT
    CTGCAGGTCC GAGGGGCGAG AGGCCACCAC TGCCATCGAG AGGCGGCCAC CACTGCCATC
    GGAGGTGGGG GTGGCCCGGG TGGAGGTGGC GGCGGGGCCA CCGATGAGGG AGGTGGCAGA
    GGCGGCGGCA GTGGTGATGG TGGTGAGGCC TGTGGCCACC CTGAGCCCCG GGGGGCCCCG
    AGTGCCCCTG GAGAGTGTGC GTCAGATCTA CAGCGAACAC AACTACTGCC GCCTTGTCCT
    CTAAACGGGG ATCACGCCCA GGCCGAAACT GCTGCGTCCA GAGCCAGGAG AGAGGACCTG
    GCTTCTCTCA GAAAGGGGCA GAGCTGCCCA CTGCAGAGGG TCCCAGATGG CCCTAGCGGC
    GGGCTGGAAG ATGCCTCCCG ACTCCCCGTT GCATCCACTC GGGATCCGCC ATGCCAGGCT
    CTGCCCCCAC TGCCCTCTAG AATCCCAGAA CCTGAGAGGT TAGTTGAGCA GCTTGTGTCG
    CATCCAGAAG GTAGAACTGA ATGTGGGTCT GGTACCACTG CCTGGGAAAG GGGTGATGAG
    AAGCTAGCAC CTACCATCCC CTCAGAGAAC AGTCCTGTTC GGGCTCTGGC CGGGCCTACT
    GGATTAGAAG AAGGAACTGG CCAGGCTCCA GACAGTGGCA GTAATCCCAC CATGAAGGAT
    CCTGTGAACG TGACCCCCAG TTCCATCTCC GAATCATCCT TGGCCAGTTG CCTGCAAGAC
    AGACCATTTG ATGATGAATC AGAACTTGAT GACTCGGGCC CACCCACAAG GGAAAGTTCT
    TCTAGACAGG AAAACTTGAA AACCGAGGCT CCTGTCTCCC CTGGTGCTGC TCCTTGGAGG
    CCTGGCATGT CAAATGATGA GGCAGGGGGA CAACCTGAAC CCGACTCCAG AGAAGAGGTC
    CCGTCCATTA AGTCCCAGGT CAGAGAGAAG TGGGAGAAAG CGGCCCCCCT CATCCCAGCA
    TCGCCTGTGG GCTTGACAGC GGAGGAGGGT CTGGATCCCC CCGTCCACTT GGGTTCACTC
    TGGACAGTGC ACTCTGGCTG TGTTGACAGC AGCGGTGACT GCAGACAGCT GGAAGGTGAC
    AAGCCCAGAA TCAATGGAGA CTCGGAAGCT CTGAGTCCTC ACAGCGAGTC CACAGACACT
    GCCTCTGACT TCGAAGGCCA CTTCTCCGAG GACAGCAGTG AGGCCGAGCC TGGTGAAACC
    TTGGGGCCGA AGAGGTCCTT GGTGGCTGAG CAGGGTGAGA AACACAGTTG GAACCACTGT
    GCCTCGCTCT CCAAGGTGAA TGGTGACCTG AGTCTGGTCA CCAGGACAGA TGGGATGGTT
    GCTCCTCAGA GCTGGGTGTC TCGGGTATGT GCAGTTCCCC CAAAGATCCC AGACTCCCTG
    CTGCTGGCCA GTCCCGAGTA CCAGCCGAGA CCCATGTCCC TGGGCAGGCC TGCATCCTCA
    GTGGAGGCTG CTAACCCCCT TGTGATGCAG TTGCTGCAGG GCCACTTGCC CCTCAAGAAG
    GTTCTCCCTC CAGCCCATGG CGGCAGCAAA CCCGAACCCC CACGACTCGC ACTTACAGCA
    GAGCAGAGCC CTGGTGGCTC CCTGGGGATG GGGTCATTGC AAGGTCCTGG GGAGAACAGA
    TGTGAAGTTG GCAAGAGCAG TCCAGAGTCT CTGCTACCTG AGAGCTGTGA AGCAAGTACC
    GGCTTTGCCC GGCTGGAGGC TAGCCAGGCT CCTGGGGCGC CCCTAAAGAT GTCCAAGAAC
    GCCCCGAGTT TAGACTCCCT ATATCCAGTG ACCAATCCAG TGGCTGCCTC TGGGAAAGCA
    GAAGTGGATT TCAAAGAACA GTTATCTCCC TTCAGTTTTG AAGATCAGAA GGAAGCCCGT
    AACCTGTCCC AGTGCAGTAT TTCAACTGCT GCCCCAGGTG TGAGTCTGGG AAATCTCACT
    ACCTCGAGAG CCCCTCTTTT CTCATCTCCA AATGCGGTCT CCTCGGGTCC TGATCAGGCA
    GGTCGGGCCC TGGGGGATCA GAACAGTGCG GGAGGCCAAG GGAAGAAGCT CTTTGGCTCC
    AAGAACGCAG CTGCAGCCCT TCAGTGCCCC CGGCTGGTGG AGCCGACGCC ACTGCCTGCG
    GAGGCCCCTC CTGCTTTTCC CAATAGGAAG TCAGGGCCAG GCAAAACCTC TCTGTCTGGT
    GGGATACAGA CTGCCAGGGA AGACTGGGCC CCAAAGCCAC CGCCTGCCTC TGTTGGCAGC
    ATCAAGAGCG AAAAGACTCT TGATGGAGGG CCTCTCAAGG TGGATGCAGA GAACAGAAAG
    GCAGTGGGGC CTGGTCCCCG GGAGCTGGTG GATCACTTGC AAGGGATGCC CTTTGTCCTT
    GATTTGCCCT TCTGGAAATT ACCCCGGGAG CCCGGGAAGG GACTCAGTCA GCCTCTGGAG
    CCTTCTTCCA TCCCCTCCCA ACTCAACATC AAGCAGGCAT TTTATGGGAA GCTTTCCAAA
    CTCCAGCTAA GTTCCACCAG CTTTAATTAT TCCTCCGGCT CCCCCCCTTT TCCCAAAGGC
    CTGGCCGGAA GTGTGGTGCA GCTGAGCCAC AAAGCAAACT TTGGTGCGAG CCACAGTGCG
    TCCCTCTCCT TGCAAATGTT CACCGACAGC AGCACAGTGG AAAGCATCTC GCTCCAGTGT
    GCGTGCAGCC TGAAAGCCAT GATCATGTGC CAGGGCTGCG GTGCATTCTG TCACGATGAC
    TGTATTGGAC CCTCAAAGCT CTGTGTATTG TGCCTTGTGG TGAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024856952.1
    CDS72..4679
    Translation

    Target ORF information:

    RefSeq Version XM_025001184.1
    Organism Bos taurus(cattle)
    Definition Bos taurus additional sex combs like 1, transcriptional regulator (ASXL1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025001184.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAAGGACA AACAGAAGAG GAAGAAGGAG CGCACGTGGG CCGAGGCCGC GCGCCTGGTA 
    CTGGAAAACT ATTCAGATGC TCCAATGACA CCCAAACAGA TTCTGCAGGT CATAGAGGCA
    GAAGGACTAA AGGAAATGAG AAGTGGGACA TCCCCTCTCG CATGTCTTAA TGCCATGCTA
    CATTCCAATT CCAGAGGAGG AGAGGGGTTA TTTTATAAAC TACCTGGCCG AATCAGCCTT
    TTTACACTCA AGAAGGATGC CCTGCAGTGG TCTCGCAGTC CAGCTGTGGT GGAGGGAGAG
    GAGCCAGAGG ACACAGCTGA TGTGGAGAGC TGTGGGTCTA ATGAAGCCAG CACTGTGAGT
    GGTGAAAATG ATGTTTCTCT CGATGAAACA TCTTCTAATG CTTCCTGTTC TACGGAATCT
    CAGAGTCGGC CTCTTTCCAA CCCCAGGGAC AGCTGCAGAG CTTCCTCACA GGCAAACAAG
    CAGAAGAAAA AGACAGGGGT GATGCTGCCT CGAGTTGTCC TGACTCCTCT GAAGGTAAAC
    GGGGCCCACG TGGAATCAGC ATCAGGGTTC TCGGGCCGCC ACGCCGATGG CGAGAGCGGC
    AGCCCGTCCA GCAGCAGCGG CGGCTCTCCG GCCCTGGGCA GCGCTGCTAT CCGTGGCCAG
    GCCGAGCTCG CCCGGGACCC TGCCCCGCTC CTGAGAGGCT TCCGGAAGCC GGCCACAGGT
    CAAATGAAGC GCAACAGAGG GGAAGAGATC GATTTTGAGA CGCCTGGGTC CATTCTCGTC
    AACACCAACC TCCGGGCCCT GATCAACTCG CGGACCTTCC ATGCCCTGCC GTCCCACTTC
    CAGCAGCAGC TCCTCTTCCT CCTGCCCGAA GTAGACAGAC AGGTGGGAAC TGATGGCCTG
    TTACGTCTCA GCAGTAGTGC GCTGAACAAT GAGTTTTTCA CCCACGCAGC TCAGAGCTGG
    CGGGAACGCC TGGCTGATGG TGAATTCACT CATGAGATGC AGGTCAGGAT ACGACAAGAA
    ATGGAGAAGG AAAAGAAAGT GGAACAATGG AAAGAAAAGT TTTTCGAAGA CTACTATGGA
    CAGAAGCTGG GTTTAACCAA AGAAGAGTCA TTGCAGCAGA ATGTGGGCCA GGAGGAGGCT
    GAAATCAAGA GTAACCTGCG TGTCTCAGAA TCAACACGGC CACAGCGTGG CCCGGCCACC
    CGGCAGCGAG ATGGGCATTT CAAGAAACGC TCTCGGCCAG ATCTTCGAAC CAGAGCCCGA
    AGGAATCTGT ACAAAAGACA GGAGCCAGAA CAAGCAGAGA TCGCTAAAGA CGCACAGTCT
    GTGACACTAG ACATCCCCCT CCACAAGGAC GGGGAGGCTA AGACCGACGC AGCAGGGGTG
    GGCAGCCCCC ACCCACCTGG CACGTCCTCC GCAGCATCCA ACCCAGAGAG TCCCGAATTC
    CCAGTGGAAA CTGTGGCCTC CCGGCTCCAG CCCAGTCCCA ATGACCAGGC ACGTGTCTCC
    GCGTCTCCAG ACAGAATTCC CAGCTTGCTG CAGGAGACCG TGGATCAGGA ACCCAAGGAT
    CAGAAGAGGA AATCCTTTGA GCAGGCGACC TCTGCCTCCT TTCCCGAAAA GAAGCCCCGG
    CTTGAAGATC GTCAGTCCTT TCGTAACACA ATTGAAAGTG TTCACCCCGA AAAGCCACAG
    CCCACCAAAG AGGAGCCCAA AGTCCCGCCC ATCCGGATTC AACTTTCACG TATCAAACCA
    CCCTGGGTGG TTAAAGGTCA GCCCACTTAC CAGATATGTC CCCGCATCGT CCCCATCACG
    GAGTCCTCCT GCCGGGGCTG GACTGGTGCC AGGACCCTTG CAGACATTAA AGCCCGTGCT
    CTGCAGGTCC GAGGGGCGAG AGGCCACCAC TGCCATCGAG AGGCGGCCAC CACTGCCATC
    GGAGGTGGGG GTGGCCCGGG TGGAGGTGGC GGCGGGGCCA CCGATGAGGG AGGTGGCAGA
    GGCGGCGGCA GTGGTGATGG TGGTGAGGCC TGTGGCCACC CTGAGCCCCG GGGGGCCCCG
    AGTGCCCCTG GAGAGTGTGC GTCAGATCTA CAGCGAACAC AACTACTGCC GCCTTGTCCT
    CTAAACGGGG ATCACGCCCA GGCCGAAACT GCTGCGTCCA GAGCCAGGAG AGAGGACCTG
    GCTTCTCTCA GAAAGGGGCA GAGCTGCCCA CTGCAGAGGG TCCCAGATGG CCCTAGCGGC
    GGGCTGGAAG ATGCCTCCCG ACTCCCCGTT GCATCCACTC GGGATCCGCC ATGCCAGGCT
    CTGCCCCCAC TGCCCTCTAG AATCCCAGAA CCTGAGAGGT TAGTTGAGCA GCTTGTGTCG
    CATCCAGAAG GTAGAACTGA ATGTGGGTCT GGTACCACTG CCTGGGAAAG GGGTGATGAG
    AAGCTAGCAC CTACCATCCC CTCAGAGAAC AGTCCTGTTC GGGCTCTGGC CGGGCCTACT
    GGATTAGAAG AAGGAACTGG CCAGGCTCCA GACAGTGGCA GTAATCCCAC CATGAAGGAT
    CCTGTGAACG TGACCCCCAG TTCCATCTCC GAATCATCCT TGGCCAGTTG CCTGCAAGAC
    AGACCATTTG ATGATGAATC AGAACTTGAT GACTCGGGCC CACCCACAAG GGAAAGTTCT
    TCTAGACAGG AAAACTTGAA AACCGAGGCT CCTGTCTCCC CTGGTGCTGC TCCTTGGAGG
    CCTGGCATGT CAAATGATGA GGCAGGGGGA CAACCTGAAC CCGACTCCAG AGAAGAGGTC
    CCGTCCATTA AGTCCCAGGT CAGAGAGAAG TGGGAGAAAG CGGCCCCCCT CATCCCAGCA
    TCGCCTGTGG GCTTGACAGC GGAGGAGGGT CTGGATCCCC CCGTCCACTT GGGTTCACTC
    TGGACAGTGC ACTCTGGCTG TGTTGACAGC AGCGGTGACT GCAGACAGCT GGAAGGTGAC
    AAGCCCAGAA TCAATGGAGA CTCGGAAGCT CTGAGTCCTC ACAGCGAGTC CACAGACACT
    GCCTCTGACT TCGAAGGCCA CTTCTCCGAG GACAGCAGTG AGGCCGAGCC TGGTGAAACC
    TTGGGGCCGA AGAGGTCCTT GGTGGCTGAG CAGGGTGAGA AACACAGTTG GAACCACTGT
    GCCTCGCTCT CCAAGGTGAA TGGTGACCTG AGTCTGGTCA CCAGGACAGA TGGGATGGTT
    GCTCCTCAGA GCTGGGTGTC TCGGGTATGT GCAGTTCCCC CAAAGATCCC AGACTCCCTG
    CTGCTGGCCA GTCCCGAGTA CCAGCCGAGA CCCATGTCCC TGGGCAGGCC TGCATCCTCA
    GTGGAGGCTG CTAACCCCCT TGTGATGCAG TTGCTGCAGG GCCACTTGCC CCTCAAGAAG
    GTTCTCCCTC CAGCCCATGG CGGCAGCAAA CCCGAACCCC CACGACTCGC ACTTACAGCA
    GAGCAGAGCC CTGGTGGCTC CCTGGGGATG GGGTCATTGC AAGGTCCTGG GGAGAACAGA
    TGTGAAGTTG GCAAGAGCAG TCCAGAGTCT CTGCTACCTG AGAGCTGTGA AGCAAGTACC
    GGCTTTGCCC GGCTGGAGGC TAGCCAGGCT CCTGGGGCGC CCCTAAAGAT GTCCAAGAAC
    GCCCCGAGTT TAGACTCCCT ATATCCAGTG ACCAATCCAG TGGCTGCCTC TGGGAAAGCA
    GAAGTGGATT TCAAAGAACA GTTATCTCCC TTCAGTTTTG AAGATCAGAA GGAAGCCCGT
    AACCTGTCCC AGTGCAGTAT TTCAACTGCT GCCCCAGGTG TGAGTCTGGG AAATCTCACT
    ACCTCGAGAG CCCCTCTTTT CTCATCTCCA AATGCGGTCT CCTCGGGTCC TGATCAGGCA
    GGTCGGGCCC TGGGGGATCA GAACAGTGCG GGAGGCCAAG GGAAGAAGCT CTTTGGCTCC
    AAGAACGCAG CTGCAGCCCT TCAGTGCCCC CGGCTGGTGG AGCCGACGCC ACTGCCTGCG
    GAGGCCCCTC CTGCTTTTCC CAATAGGAAG TCAGGGCCAG GCAAAACCTC TCTGTCTGGT
    GGGATACAGA CTGCCAGGGA AGACTGGGCC CCAAAGCCAC CGCCTGCCTC TGTTGGCAGC
    ATCAAGAGCG AAAAGACTCT TGATGGAGGG CCTCTCAAGG TGGATGCAGA GAACAGAAAG
    GCAGTGGGGC CTGGTCCCCG GGAGCTGGTG GATCACTTGC AAGGGATGCC CTTTGTCCTT
    GATTTGCCCT TCTGGAAATT ACCCCGGGAG CCCGGGAAGG GACTCAGTCA GCCTCTGGAG
    CCTTCTTCCA TCCCCTCCCA ACTCAACATC AAGCAGGCAT TTTATGGGAA GCTTTCCAAA
    CTCCAGCTAA GTTCCACCAG CTTTAATTAT TCCTCCGGCT CCCCCCCTTT TCCCAAAGGC
    CTGGCCGGAA GTGTGGTGCA GCTGAGCCAC AAAGCAAACT TTGGTGCGAG CCACAGTGCG
    TCCCTCTCCT TGCAAATGTT CACCGACAGC AGCACAGTGG AAAGCATCTC GCTCCAGTGT
    GCGTGCAGCC TGAAAGCCAT GATCATGTGC CAGGGCTGCG GTGCATTCTG TCACGATGAC
    TGTATTGGAC CCTCAAAGCT CTGTGTATTG TGCCTTGTGG TGAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222036
    Clone ID Related Accession (Same CDS sequence) XM_025001185.1
    Accession Version XM_025001185.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4605bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product putative Polycomb group protein ASXL1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAAGGACA AACAGAAGAG GAAGAAGGAG CGCACGTGGG CCGAGGCCGC GCGCCTGGTA 
    CTGGAAAACT ATTCAGATGC TCCAATGACA CCCAAACAGA TTCTGCAGGT CATAGAGGCA
    GAAGGACTAA AGGAAATGAG TGGGACATCC CCTCTCGCAT GTCTTAATGC CATGCTACAT
    TCCAATTCCA GAGGAGGAGA GGGGTTATTT TATAAACTAC CTGGCCGAAT CAGCCTTTTT
    ACACTCAAGA AGGATGCCCT GCAGTGGTCT CGCAGTCCAG CTGTGGTGGA GGGAGAGGAG
    CCAGAGGACA CAGCTGATGT GGAGAGCTGT GGGTCTAATG AAGCCAGCAC TGTGAGTGGT
    GAAAATGATG TTTCTCTCGA TGAAACATCT TCTAATGCTT CCTGTTCTAC GGAATCTCAG
    AGTCGGCCTC TTTCCAACCC CAGGGACAGC TGCAGAGCTT CCTCACAGGC AAACAAGCAG
    AAGAAAAAGA CAGGGGTGAT GCTGCCTCGA GTTGTCCTGA CTCCTCTGAA GGTAAACGGG
    GCCCACGTGG AATCAGCATC AGGGTTCTCG GGCCGCCACG CCGATGGCGA GAGCGGCAGC
    CCGTCCAGCA GCAGCGGCGG CTCTCCGGCC CTGGGCAGCG CTGCTATCCG TGGCCAGGCC
    GAGCTCGCCC GGGACCCTGC CCCGCTCCTG AGAGGCTTCC GGAAGCCGGC CACAGGTCAA
    ATGAAGCGCA ACAGAGGGGA AGAGATCGAT TTTGAGACGC CTGGGTCCAT TCTCGTCAAC
    ACCAACCTCC GGGCCCTGAT CAACTCGCGG ACCTTCCATG CCCTGCCGTC CCACTTCCAG
    CAGCAGCTCC TCTTCCTCCT GCCCGAAGTA GACAGACAGG TGGGAACTGA TGGCCTGTTA
    CGTCTCAGCA GTAGTGCGCT GAACAATGAG TTTTTCACCC ACGCAGCTCA GAGCTGGCGG
    GAACGCCTGG CTGATGGTGA ATTCACTCAT GAGATGCAGG TCAGGATACG ACAAGAAATG
    GAGAAGGAAA AGAAAGTGGA ACAATGGAAA GAAAAGTTTT TCGAAGACTA CTATGGACAG
    AAGCTGGGTT TAACCAAAGA AGAGTCATTG CAGCAGAATG TGGGCCAGGA GGAGGCTGAA
    ATCAAGAGTA ACCTGCGTGT CTCAGAATCA ACACGGCCAC AGCGTGGCCC GGCCACCCGG
    CAGCGAGATG GGCATTTCAA GAAACGCTCT CGGCCAGATC TTCGAACCAG AGCCCGAAGG
    AATCTGTACA AAAGACAGGA GCCAGAACAA GCAGAGATCG CTAAAGACGC ACAGTCTGTG
    ACACTAGACA TCCCCCTCCA CAAGGACGGG GAGGCTAAGA CCGACGCAGC AGGGGTGGGC
    AGCCCCCACC CACCTGGCAC GTCCTCCGCA GCATCCAACC CAGAGAGTCC CGAATTCCCA
    GTGGAAACTG TGGCCTCCCG GCTCCAGCCC AGTCCCAATG ACCAGGCACG TGTCTCCGCG
    TCTCCAGACA GAATTCCCAG CTTGCTGCAG GAGACCGTGG ATCAGGAACC CAAGGATCAG
    AAGAGGAAAT CCTTTGAGCA GGCGACCTCT GCCTCCTTTC CCGAAAAGAA GCCCCGGCTT
    GAAGATCGTC AGTCCTTTCG TAACACAATT GAAAGTGTTC ACCCCGAAAA GCCACAGCCC
    ACCAAAGAGG AGCCCAAAGT CCCGCCCATC CGGATTCAAC TTTCACGTAT CAAACCACCC
    TGGGTGGTTA AAGGTCAGCC CACTTACCAG ATATGTCCCC GCATCGTCCC CATCACGGAG
    TCCTCCTGCC GGGGCTGGAC TGGTGCCAGG ACCCTTGCAG ACATTAAAGC CCGTGCTCTG
    CAGGTCCGAG GGGCGAGAGG CCACCACTGC CATCGAGAGG CGGCCACCAC TGCCATCGGA
    GGTGGGGGTG GCCCGGGTGG AGGTGGCGGC GGGGCCACCG ATGAGGGAGG TGGCAGAGGC
    GGCGGCAGTG GTGATGGTGG TGAGGCCTGT GGCCACCCTG AGCCCCGGGG GGCCCCGAGT
    GCCCCTGGAG AGTGTGCGTC AGATCTACAG CGAACACAAC TACTGCCGCC TTGTCCTCTA
    AACGGGGATC ACGCCCAGGC CGAAACTGCT GCGTCCAGAG CCAGGAGAGA GGACCTGGCT
    TCTCTCAGAA AGGGGCAGAG CTGCCCACTG CAGAGGGTCC CAGATGGCCC TAGCGGCGGG
    CTGGAAGATG CCTCCCGACT CCCCGTTGCA TCCACTCGGG ATCCGCCATG CCAGGCTCTG
    CCCCCACTGC CCTCTAGAAT CCCAGAACCT GAGAGGTTAG TTGAGCAGCT TGTGTCGCAT
    CCAGAAGGTA GAACTGAATG TGGGTCTGGT ACCACTGCCT GGGAAAGGGG TGATGAGAAG
    CTAGCACCTA CCATCCCCTC AGAGAACAGT CCTGTTCGGG CTCTGGCCGG GCCTACTGGA
    TTAGAAGAAG GAACTGGCCA GGCTCCAGAC AGTGGCAGTA ATCCCACCAT GAAGGATCCT
    GTGAACGTGA CCCCCAGTTC CATCTCCGAA TCATCCTTGG CCAGTTGCCT GCAAGACAGA
    CCATTTGATG ATGAATCAGA ACTTGATGAC TCGGGCCCAC CCACAAGGGA AAGTTCTTCT
    AGACAGGAAA ACTTGAAAAC CGAGGCTCCT GTCTCCCCTG GTGCTGCTCC TTGGAGGCCT
    GGCATGTCAA ATGATGAGGC AGGGGGACAA CCTGAACCCG ACTCCAGAGA AGAGGTCCCG
    TCCATTAAGT CCCAGGTCAG AGAGAAGTGG GAGAAAGCGG CCCCCCTCAT CCCAGCATCG
    CCTGTGGGCT TGACAGCGGA GGAGGGTCTG GATCCCCCCG TCCACTTGGG TTCACTCTGG
    ACAGTGCACT CTGGCTGTGT TGACAGCAGC GGTGACTGCA GACAGCTGGA AGGTGACAAG
    CCCAGAATCA ATGGAGACTC GGAAGCTCTG AGTCCTCACA GCGAGTCCAC AGACACTGCC
    TCTGACTTCG AAGGCCACTT CTCCGAGGAC AGCAGTGAGG CCGAGCCTGG TGAAACCTTG
    GGGCCGAAGA GGTCCTTGGT GGCTGAGCAG GGTGAGAAAC ACAGTTGGAA CCACTGTGCC
    TCGCTCTCCA AGGTGAATGG TGACCTGAGT CTGGTCACCA GGACAGATGG GATGGTTGCT
    CCTCAGAGCT GGGTGTCTCG GGTATGTGCA GTTCCCCCAA AGATCCCAGA CTCCCTGCTG
    CTGGCCAGTC CCGAGTACCA GCCGAGACCC ATGTCCCTGG GCAGGCCTGC ATCCTCAGTG
    GAGGCTGCTA ACCCCCTTGT GATGCAGTTG CTGCAGGGCC ACTTGCCCCT CAAGAAGGTT
    CTCCCTCCAG CCCATGGCGG CAGCAAACCC GAACCCCCAC GACTCGCACT TACAGCAGAG
    CAGAGCCCTG GTGGCTCCCT GGGGATGGGG TCATTGCAAG GTCCTGGGGA GAACAGATGT
    GAAGTTGGCA AGAGCAGTCC AGAGTCTCTG CTACCTGAGA GCTGTGAAGC AAGTACCGGC
    TTTGCCCGGC TGGAGGCTAG CCAGGCTCCT GGGGCGCCCC TAAAGATGTC CAAGAACGCC
    CCGAGTTTAG ACTCCCTATA TCCAGTGACC AATCCAGTGG CTGCCTCTGG GAAAGCAGAA
    GTGGATTTCA AAGAACAGTT ATCTCCCTTC AGTTTTGAAG ATCAGAAGGA AGCCCGTAAC
    CTGTCCCAGT GCAGTATTTC AACTGCTGCC CCAGGTGTGA GTCTGGGAAA TCTCACTACC
    TCGAGAGCCC CTCTTTTCTC ATCTCCAAAT GCGGTCTCCT CGGGTCCTGA TCAGGCAGGT
    CGGGCCCTGG GGGATCAGAA CAGTGCGGGA GGCCAAGGGA AGAAGCTCTT TGGCTCCAAG
    AACGCAGCTG CAGCCCTTCA GTGCCCCCGG CTGGTGGAGC CGACGCCACT GCCTGCGGAG
    GCCCCTCCTG CTTTTCCCAA TAGGAAGTCA GGGCCAGGCA AAACCTCTCT GTCTGGTGGG
    ATACAGACTG CCAGGGAAGA CTGGGCCCCA AAGCCACCGC CTGCCTCTGT TGGCAGCATC
    AAGAGCGAAA AGACTCTTGA TGGAGGGCCT CTCAAGGTGG ATGCAGAGAA CAGAAAGGCA
    GTGGGGCCTG GTCCCCGGGA GCTGGTGGAT CACTTGCAAG GGATGCCCTT TGTCCTTGAT
    TTGCCCTTCT GGAAATTACC CCGGGAGCCC GGGAAGGGAC TCAGTCAGCC TCTGGAGCCT
    TCTTCCATCC CCTCCCAACT CAACATCAAG CAGGCATTTT ATGGGAAGCT TTCCAAACTC
    CAGCTAAGTT CCACCAGCTT TAATTATTCC TCCGGCTCCC CCCCTTTTCC CAAAGGCCTG
    GCCGGAAGTG TGGTGCAGCT GAGCCACAAA GCAAACTTTG GTGCGAGCCA CAGTGCGTCC
    CTCTCCTTGC AAATGTTCAC CGACAGCAGC ACAGTGGAAA GCATCTCGCT CCAGTGTGCG
    TGCAGCCTGA AAGCCATGAT CATGTGCCAG GGCTGCGGTG CATTCTGTCA CGATGACTGT
    ATTGGACCCT CAAAGCTCTG TGTATTGTGC CTTGTGGTGA GATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024856953.1
    CDS72..4676
    Translation

    Target ORF information:

    RefSeq Version XM_025001185.1
    Organism Bos taurus(cattle)
    Definition Bos taurus additional sex combs like 1, transcriptional regulator (ASXL1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025001185.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAAGGACA AACAGAAGAG GAAGAAGGAG CGCACGTGGG CCGAGGCCGC GCGCCTGGTA 
    CTGGAAAACT ATTCAGATGC TCCAATGACA CCCAAACAGA TTCTGCAGGT CATAGAGGCA
    GAAGGACTAA AGGAAATGAG TGGGACATCC CCTCTCGCAT GTCTTAATGC CATGCTACAT
    TCCAATTCCA GAGGAGGAGA GGGGTTATTT TATAAACTAC CTGGCCGAAT CAGCCTTTTT
    ACACTCAAGA AGGATGCCCT GCAGTGGTCT CGCAGTCCAG CTGTGGTGGA GGGAGAGGAG
    CCAGAGGACA CAGCTGATGT GGAGAGCTGT GGGTCTAATG AAGCCAGCAC TGTGAGTGGT
    GAAAATGATG TTTCTCTCGA TGAAACATCT TCTAATGCTT CCTGTTCTAC GGAATCTCAG
    AGTCGGCCTC TTTCCAACCC CAGGGACAGC TGCAGAGCTT CCTCACAGGC AAACAAGCAG
    AAGAAAAAGA CAGGGGTGAT GCTGCCTCGA GTTGTCCTGA CTCCTCTGAA GGTAAACGGG
    GCCCACGTGG AATCAGCATC AGGGTTCTCG GGCCGCCACG CCGATGGCGA GAGCGGCAGC
    CCGTCCAGCA GCAGCGGCGG CTCTCCGGCC CTGGGCAGCG CTGCTATCCG TGGCCAGGCC
    GAGCTCGCCC GGGACCCTGC CCCGCTCCTG AGAGGCTTCC GGAAGCCGGC CACAGGTCAA
    ATGAAGCGCA ACAGAGGGGA AGAGATCGAT TTTGAGACGC CTGGGTCCAT TCTCGTCAAC
    ACCAACCTCC GGGCCCTGAT CAACTCGCGG ACCTTCCATG CCCTGCCGTC CCACTTCCAG
    CAGCAGCTCC TCTTCCTCCT GCCCGAAGTA GACAGACAGG TGGGAACTGA TGGCCTGTTA
    CGTCTCAGCA GTAGTGCGCT GAACAATGAG TTTTTCACCC ACGCAGCTCA GAGCTGGCGG
    GAACGCCTGG CTGATGGTGA ATTCACTCAT GAGATGCAGG TCAGGATACG ACAAGAAATG
    GAGAAGGAAA AGAAAGTGGA ACAATGGAAA GAAAAGTTTT TCGAAGACTA CTATGGACAG
    AAGCTGGGTT TAACCAAAGA AGAGTCATTG CAGCAGAATG TGGGCCAGGA GGAGGCTGAA
    ATCAAGAGTA ACCTGCGTGT CTCAGAATCA ACACGGCCAC AGCGTGGCCC GGCCACCCGG
    CAGCGAGATG GGCATTTCAA GAAACGCTCT CGGCCAGATC TTCGAACCAG AGCCCGAAGG
    AATCTGTACA AAAGACAGGA GCCAGAACAA GCAGAGATCG CTAAAGACGC ACAGTCTGTG
    ACACTAGACA TCCCCCTCCA CAAGGACGGG GAGGCTAAGA CCGACGCAGC AGGGGTGGGC
    AGCCCCCACC CACCTGGCAC GTCCTCCGCA GCATCCAACC CAGAGAGTCC CGAATTCCCA
    GTGGAAACTG TGGCCTCCCG GCTCCAGCCC AGTCCCAATG ACCAGGCACG TGTCTCCGCG
    TCTCCAGACA GAATTCCCAG CTTGCTGCAG GAGACCGTGG ATCAGGAACC CAAGGATCAG
    AAGAGGAAAT CCTTTGAGCA GGCGACCTCT GCCTCCTTTC CCGAAAAGAA GCCCCGGCTT
    GAAGATCGTC AGTCCTTTCG TAACACAATT GAAAGTGTTC ACCCCGAAAA GCCACAGCCC
    ACCAAAGAGG AGCCCAAAGT CCCGCCCATC CGGATTCAAC TTTCACGTAT CAAACCACCC
    TGGGTGGTTA AAGGTCAGCC CACTTACCAG ATATGTCCCC GCATCGTCCC CATCACGGAG
    TCCTCCTGCC GGGGCTGGAC TGGTGCCAGG ACCCTTGCAG ACATTAAAGC CCGTGCTCTG
    CAGGTCCGAG GGGCGAGAGG CCACCACTGC CATCGAGAGG CGGCCACCAC TGCCATCGGA
    GGTGGGGGTG GCCCGGGTGG AGGTGGCGGC GGGGCCACCG ATGAGGGAGG TGGCAGAGGC
    GGCGGCAGTG GTGATGGTGG TGAGGCCTGT GGCCACCCTG AGCCCCGGGG GGCCCCGAGT
    GCCCCTGGAG AGTGTGCGTC AGATCTACAG CGAACACAAC TACTGCCGCC TTGTCCTCTA
    AACGGGGATC ACGCCCAGGC CGAAACTGCT GCGTCCAGAG CCAGGAGAGA GGACCTGGCT
    TCTCTCAGAA AGGGGCAGAG CTGCCCACTG CAGAGGGTCC CAGATGGCCC TAGCGGCGGG
    CTGGAAGATG CCTCCCGACT CCCCGTTGCA TCCACTCGGG ATCCGCCATG CCAGGCTCTG
    CCCCCACTGC CCTCTAGAAT CCCAGAACCT GAGAGGTTAG TTGAGCAGCT TGTGTCGCAT
    CCAGAAGGTA GAACTGAATG TGGGTCTGGT ACCACTGCCT GGGAAAGGGG TGATGAGAAG
    CTAGCACCTA CCATCCCCTC AGAGAACAGT CCTGTTCGGG CTCTGGCCGG GCCTACTGGA
    TTAGAAGAAG GAACTGGCCA GGCTCCAGAC AGTGGCAGTA ATCCCACCAT GAAGGATCCT
    GTGAACGTGA CCCCCAGTTC CATCTCCGAA TCATCCTTGG CCAGTTGCCT GCAAGACAGA
    CCATTTGATG ATGAATCAGA ACTTGATGAC TCGGGCCCAC CCACAAGGGA AAGTTCTTCT
    AGACAGGAAA ACTTGAAAAC CGAGGCTCCT GTCTCCCCTG GTGCTGCTCC TTGGAGGCCT
    GGCATGTCAA ATGATGAGGC AGGGGGACAA CCTGAACCCG ACTCCAGAGA AGAGGTCCCG
    TCCATTAAGT CCCAGGTCAG AGAGAAGTGG GAGAAAGCGG CCCCCCTCAT CCCAGCATCG
    CCTGTGGGCT TGACAGCGGA GGAGGGTCTG GATCCCCCCG TCCACTTGGG TTCACTCTGG
    ACAGTGCACT CTGGCTGTGT TGACAGCAGC GGTGACTGCA GACAGCTGGA AGGTGACAAG
    CCCAGAATCA ATGGAGACTC GGAAGCTCTG AGTCCTCACA GCGAGTCCAC AGACACTGCC
    TCTGACTTCG AAGGCCACTT CTCCGAGGAC AGCAGTGAGG CCGAGCCTGG TGAAACCTTG
    GGGCCGAAGA GGTCCTTGGT GGCTGAGCAG GGTGAGAAAC ACAGTTGGAA CCACTGTGCC
    TCGCTCTCCA AGGTGAATGG TGACCTGAGT CTGGTCACCA GGACAGATGG GATGGTTGCT
    CCTCAGAGCT GGGTGTCTCG GGTATGTGCA GTTCCCCCAA AGATCCCAGA CTCCCTGCTG
    CTGGCCAGTC CCGAGTACCA GCCGAGACCC ATGTCCCTGG GCAGGCCTGC ATCCTCAGTG
    GAGGCTGCTA ACCCCCTTGT GATGCAGTTG CTGCAGGGCC ACTTGCCCCT CAAGAAGGTT
    CTCCCTCCAG CCCATGGCGG CAGCAAACCC GAACCCCCAC GACTCGCACT TACAGCAGAG
    CAGAGCCCTG GTGGCTCCCT GGGGATGGGG TCATTGCAAG GTCCTGGGGA GAACAGATGT
    GAAGTTGGCA AGAGCAGTCC AGAGTCTCTG CTACCTGAGA GCTGTGAAGC AAGTACCGGC
    TTTGCCCGGC TGGAGGCTAG CCAGGCTCCT GGGGCGCCCC TAAAGATGTC CAAGAACGCC
    CCGAGTTTAG ACTCCCTATA TCCAGTGACC AATCCAGTGG CTGCCTCTGG GAAAGCAGAA
    GTGGATTTCA AAGAACAGTT ATCTCCCTTC AGTTTTGAAG ATCAGAAGGA AGCCCGTAAC
    CTGTCCCAGT GCAGTATTTC AACTGCTGCC CCAGGTGTGA GTCTGGGAAA TCTCACTACC
    TCGAGAGCCC CTCTTTTCTC ATCTCCAAAT GCGGTCTCCT CGGGTCCTGA TCAGGCAGGT
    CGGGCCCTGG GGGATCAGAA CAGTGCGGGA GGCCAAGGGA AGAAGCTCTT TGGCTCCAAG
    AACGCAGCTG CAGCCCTTCA GTGCCCCCGG CTGGTGGAGC CGACGCCACT GCCTGCGGAG
    GCCCCTCCTG CTTTTCCCAA TAGGAAGTCA GGGCCAGGCA AAACCTCTCT GTCTGGTGGG
    ATACAGACTG CCAGGGAAGA CTGGGCCCCA AAGCCACCGC CTGCCTCTGT TGGCAGCATC
    AAGAGCGAAA AGACTCTTGA TGGAGGGCCT CTCAAGGTGG ATGCAGAGAA CAGAAAGGCA
    GTGGGGCCTG GTCCCCGGGA GCTGGTGGAT CACTTGCAAG GGATGCCCTT TGTCCTTGAT
    TTGCCCTTCT GGAAATTACC CCGGGAGCCC GGGAAGGGAC TCAGTCAGCC TCTGGAGCCT
    TCTTCCATCC CCTCCCAACT CAACATCAAG CAGGCATTTT ATGGGAAGCT TTCCAAACTC
    CAGCTAAGTT CCACCAGCTT TAATTATTCC TCCGGCTCCC CCCCTTTTCC CAAAGGCCTG
    GCCGGAAGTG TGGTGCAGCT GAGCCACAAA GCAAACTTTG GTGCGAGCCA CAGTGCGTCC
    CTCTCCTTGC AAATGTTCAC CGACAGCAGC ACAGTGGAAA GCATCTCGCT CCAGTGTGCG
    TGCAGCCTGA AAGCCATGAT CATGTGCCAG GGCTGCGGTG CATTCTGTCA CGATGACTGT
    ATTGGACCCT CAAAGCTCTG TGTATTGTGC CTTGTGGTGA GATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222037
    Clone ID Related Accession (Same CDS sequence) XM_025001186.1
    Accession Version XM_025001186.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4602bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product putative Polycomb group protein ASXL1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGACTCTGG GCACCAAAAG GAAATTTGCT TGCCAGAGTT TTTACTTGTT AGAGGTACTG 
    GAAAACTATT CAGATGCTCC AATGACACCC AAACAGATTC TGCAGGTCAT AGAGGCAGAA
    GGACTAAAGG AAATGAGTGG GACATCCCCT CTCGCATGTC TTAATGCCAT GCTACATTCC
    AATTCCAGAG GAGGAGAGGG GTTATTTTAT AAACTACCTG GCCGAATCAG CCTTTTTACA
    CTCAAGAAGG ATGCCCTGCA GTGGTCTCGC AGTCCAGCTG TGGTGGAGGG AGAGGAGCCA
    GAGGACACAG CTGATGTGGA GAGCTGTGGG TCTAATGAAG CCAGCACTGT GAGTGGTGAA
    AATGATGTTT CTCTCGATGA AACATCTTCT AATGCTTCCT GTTCTACGGA ATCTCAGAGT
    CGGCCTCTTT CCAACCCCAG GGACAGCTGC AGAGCTTCCT CACAGGCAAA CAAGCAGAAG
    AAAAAGACAG GGGTGATGCT GCCTCGAGTT GTCCTGACTC CTCTGAAGGT AAACGGGGCC
    CACGTGGAAT CAGCATCAGG GTTCTCGGGC CGCCACGCCG ATGGCGAGAG CGGCAGCCCG
    TCCAGCAGCA GCGGCGGCTC TCCGGCCCTG GGCAGCGCTG CTATCCGTGG CCAGGCCGAG
    CTCGCCCGGG ACCCTGCCCC GCTCCTGAGA GGCTTCCGGA AGCCGGCCAC AGGTCAAATG
    AAGCGCAACA GAGGGGAAGA GATCGATTTT GAGACGCCTG GGTCCATTCT CGTCAACACC
    AACCTCCGGG CCCTGATCAA CTCGCGGACC TTCCATGCCC TGCCGTCCCA CTTCCAGCAG
    CAGCTCCTCT TCCTCCTGCC CGAAGTAGAC AGACAGGTGG GAACTGATGG CCTGTTACGT
    CTCAGCAGTA GTGCGCTGAA CAATGAGTTT TTCACCCACG CAGCTCAGAG CTGGCGGGAA
    CGCCTGGCTG ATGGTGAATT CACTCATGAG ATGCAGGTCA GGATACGACA AGAAATGGAG
    AAGGAAAAGA AAGTGGAACA ATGGAAAGAA AAGTTTTTCG AAGACTACTA TGGACAGAAG
    CTGGGTTTAA CCAAAGAAGA GTCATTGCAG CAGAATGTGG GCCAGGAGGA GGCTGAAATC
    AAGAGTAACC TGCGTGTCTC AGAATCAACA CGGCCACAGC GTGGCCCGGC CACCCGGCAG
    CGAGATGGGC ATTTCAAGAA ACGCTCTCGG CCAGATCTTC GAACCAGAGC CCGAAGGAAT
    CTGTACAAAA GACAGGAGCC AGAACAAGCA GAGATCGCTA AAGACGCACA GTCTGTGACA
    CTAGACATCC CCCTCCACAA GGACGGGGAG GCTAAGACCG ACGCAGCAGG GGTGGGCAGC
    CCCCACCCAC CTGGCACGTC CTCCGCAGCA TCCAACCCAG AGAGTCCCGA ATTCCCAGTG
    GAAACTGTGG CCTCCCGGCT CCAGCCCAGT CCCAATGACC AGGCACGTGT CTCCGCGTCT
    CCAGACAGAA TTCCCAGCTT GCTGCAGGAG ACCGTGGATC AGGAACCCAA GGATCAGAAG
    AGGAAATCCT TTGAGCAGGC GACCTCTGCC TCCTTTCCCG AAAAGAAGCC CCGGCTTGAA
    GATCGTCAGT CCTTTCGTAA CACAATTGAA AGTGTTCACC CCGAAAAGCC ACAGCCCACC
    AAAGAGGAGC CCAAAGTCCC GCCCATCCGG ATTCAACTTT CACGTATCAA ACCACCCTGG
    GTGGTTAAAG GTCAGCCCAC TTACCAGATA TGTCCCCGCA TCGTCCCCAT CACGGAGTCC
    TCCTGCCGGG GCTGGACTGG TGCCAGGACC CTTGCAGACA TTAAAGCCCG TGCTCTGCAG
    GTCCGAGGGG CGAGAGGCCA CCACTGCCAT CGAGAGGCGG CCACCACTGC CATCGGAGGT
    GGGGGTGGCC CGGGTGGAGG TGGCGGCGGG GCCACCGATG AGGGAGGTGG CAGAGGCGGC
    GGCAGTGGTG ATGGTGGTGA GGCCTGTGGC CACCCTGAGC CCCGGGGGGC CCCGAGTGCC
    CCTGGAGAGT GTGCGTCAGA TCTACAGCGA ACACAACTAC TGCCGCCTTG TCCTCTAAAC
    GGGGATCACG CCCAGGCCGA AACTGCTGCG TCCAGAGCCA GGAGAGAGGA CCTGGCTTCT
    CTCAGAAAGG GGCAGAGCTG CCCACTGCAG AGGGTCCCAG ATGGCCCTAG CGGCGGGCTG
    GAAGATGCCT CCCGACTCCC CGTTGCATCC ACTCGGGATC CGCCATGCCA GGCTCTGCCC
    CCACTGCCCT CTAGAATCCC AGAACCTGAG AGGTTAGTTG AGCAGCTTGT GTCGCATCCA
    GAAGGTAGAA CTGAATGTGG GTCTGGTACC ACTGCCTGGG AAAGGGGTGA TGAGAAGCTA
    GCACCTACCA TCCCCTCAGA GAACAGTCCT GTTCGGGCTC TGGCCGGGCC TACTGGATTA
    GAAGAAGGAA CTGGCCAGGC TCCAGACAGT GGCAGTAATC CCACCATGAA GGATCCTGTG
    AACGTGACCC CCAGTTCCAT CTCCGAATCA TCCTTGGCCA GTTGCCTGCA AGACAGACCA
    TTTGATGATG AATCAGAACT TGATGACTCG GGCCCACCCA CAAGGGAAAG TTCTTCTAGA
    CAGGAAAACT TGAAAACCGA GGCTCCTGTC TCCCCTGGTG CTGCTCCTTG GAGGCCTGGC
    ATGTCAAATG ATGAGGCAGG GGGACAACCT GAACCCGACT CCAGAGAAGA GGTCCCGTCC
    ATTAAGTCCC AGGTCAGAGA GAAGTGGGAG AAAGCGGCCC CCCTCATCCC AGCATCGCCT
    GTGGGCTTGA CAGCGGAGGA GGGTCTGGAT CCCCCCGTCC ACTTGGGTTC ACTCTGGACA
    GTGCACTCTG GCTGTGTTGA CAGCAGCGGT GACTGCAGAC AGCTGGAAGG TGACAAGCCC
    AGAATCAATG GAGACTCGGA AGCTCTGAGT CCTCACAGCG AGTCCACAGA CACTGCCTCT
    GACTTCGAAG GCCACTTCTC CGAGGACAGC AGTGAGGCCG AGCCTGGTGA AACCTTGGGG
    CCGAAGAGGT CCTTGGTGGC TGAGCAGGGT GAGAAACACA GTTGGAACCA CTGTGCCTCG
    CTCTCCAAGG TGAATGGTGA CCTGAGTCTG GTCACCAGGA CAGATGGGAT GGTTGCTCCT
    CAGAGCTGGG TGTCTCGGGT ATGTGCAGTT CCCCCAAAGA TCCCAGACTC CCTGCTGCTG
    GCCAGTCCCG AGTACCAGCC GAGACCCATG TCCCTGGGCA GGCCTGCATC CTCAGTGGAG
    GCTGCTAACC CCCTTGTGAT GCAGTTGCTG CAGGGCCACT TGCCCCTCAA GAAGGTTCTC
    CCTCCAGCCC ATGGCGGCAG CAAACCCGAA CCCCCACGAC TCGCACTTAC AGCAGAGCAG
    AGCCCTGGTG GCTCCCTGGG GATGGGGTCA TTGCAAGGTC CTGGGGAGAA CAGATGTGAA
    GTTGGCAAGA GCAGTCCAGA GTCTCTGCTA CCTGAGAGCT GTGAAGCAAG TACCGGCTTT
    GCCCGGCTGG AGGCTAGCCA GGCTCCTGGG GCGCCCCTAA AGATGTCCAA GAACGCCCCG
    AGTTTAGACT CCCTATATCC AGTGACCAAT CCAGTGGCTG CCTCTGGGAA AGCAGAAGTG
    GATTTCAAAG AACAGTTATC TCCCTTCAGT TTTGAAGATC AGAAGGAAGC CCGTAACCTG
    TCCCAGTGCA GTATTTCAAC TGCTGCCCCA GGTGTGAGTC TGGGAAATCT CACTACCTCG
    AGAGCCCCTC TTTTCTCATC TCCAAATGCG GTCTCCTCGG GTCCTGATCA GGCAGGTCGG
    GCCCTGGGGG ATCAGAACAG TGCGGGAGGC CAAGGGAAGA AGCTCTTTGG CTCCAAGAAC
    GCAGCTGCAG CCCTTCAGTG CCCCCGGCTG GTGGAGCCGA CGCCACTGCC TGCGGAGGCC
    CCTCCTGCTT TTCCCAATAG GAAGTCAGGG CCAGGCAAAA CCTCTCTGTC TGGTGGGATA
    CAGACTGCCA GGGAAGACTG GGCCCCAAAG CCACCGCCTG CCTCTGTTGG CAGCATCAAG
    AGCGAAAAGA CTCTTGATGG AGGGCCTCTC AAGGTGGATG CAGAGAACAG AAAGGCAGTG
    GGGCCTGGTC CCCGGGAGCT GGTGGATCAC TTGCAAGGGA TGCCCTTTGT CCTTGATTTG
    CCCTTCTGGA AATTACCCCG GGAGCCCGGG AAGGGACTCA GTCAGCCTCT GGAGCCTTCT
    TCCATCCCCT CCCAACTCAA CATCAAGCAG GCATTTTATG GGAAGCTTTC CAAACTCCAG
    CTAAGTTCCA CCAGCTTTAA TTATTCCTCC GGCTCCCCCC CTTTTCCCAA AGGCCTGGCC
    GGAAGTGTGG TGCAGCTGAG CCACAAAGCA AACTTTGGTG CGAGCCACAG TGCGTCCCTC
    TCCTTGCAAA TGTTCACCGA CAGCAGCACA GTGGAAAGCA TCTCGCTCCA GTGTGCGTGC
    AGCCTGAAAG CCATGATCAT GTGCCAGGGC TGCGGTGCAT TCTGTCACGA TGACTGTATT
    GGACCCTCAA AGCTCTGTGT ATTGTGCCTT GTGGTGAGAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024856954.1
    CDS121..4722
    Translation

    Target ORF information:

    RefSeq Version XM_025001186.1
    Organism Bos taurus(cattle)
    Definition Bos taurus additional sex combs like 1, transcriptional regulator (ASXL1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025001186.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGACTCTGG GCACCAAAAG GAAATTTGCT TGCCAGAGTT TTTACTTGTT AGAGGTACTG 
    GAAAACTATT CAGATGCTCC AATGACACCC AAACAGATTC TGCAGGTCAT AGAGGCAGAA
    GGACTAAAGG AAATGAGTGG GACATCCCCT CTCGCATGTC TTAATGCCAT GCTACATTCC
    AATTCCAGAG GAGGAGAGGG GTTATTTTAT AAACTACCTG GCCGAATCAG CCTTTTTACA
    CTCAAGAAGG ATGCCCTGCA GTGGTCTCGC AGTCCAGCTG TGGTGGAGGG AGAGGAGCCA
    GAGGACACAG CTGATGTGGA GAGCTGTGGG TCTAATGAAG CCAGCACTGT GAGTGGTGAA
    AATGATGTTT CTCTCGATGA AACATCTTCT AATGCTTCCT GTTCTACGGA ATCTCAGAGT
    CGGCCTCTTT CCAACCCCAG GGACAGCTGC AGAGCTTCCT CACAGGCAAA CAAGCAGAAG
    AAAAAGACAG GGGTGATGCT GCCTCGAGTT GTCCTGACTC CTCTGAAGGT AAACGGGGCC
    CACGTGGAAT CAGCATCAGG GTTCTCGGGC CGCCACGCCG ATGGCGAGAG CGGCAGCCCG
    TCCAGCAGCA GCGGCGGCTC TCCGGCCCTG GGCAGCGCTG CTATCCGTGG CCAGGCCGAG
    CTCGCCCGGG ACCCTGCCCC GCTCCTGAGA GGCTTCCGGA AGCCGGCCAC AGGTCAAATG
    AAGCGCAACA GAGGGGAAGA GATCGATTTT GAGACGCCTG GGTCCATTCT CGTCAACACC
    AACCTCCGGG CCCTGATCAA CTCGCGGACC TTCCATGCCC TGCCGTCCCA CTTCCAGCAG
    CAGCTCCTCT TCCTCCTGCC CGAAGTAGAC AGACAGGTGG GAACTGATGG CCTGTTACGT
    CTCAGCAGTA GTGCGCTGAA CAATGAGTTT TTCACCCACG CAGCTCAGAG CTGGCGGGAA
    CGCCTGGCTG ATGGTGAATT CACTCATGAG ATGCAGGTCA GGATACGACA AGAAATGGAG
    AAGGAAAAGA AAGTGGAACA ATGGAAAGAA AAGTTTTTCG AAGACTACTA TGGACAGAAG
    CTGGGTTTAA CCAAAGAAGA GTCATTGCAG CAGAATGTGG GCCAGGAGGA GGCTGAAATC
    AAGAGTAACC TGCGTGTCTC AGAATCAACA CGGCCACAGC GTGGCCCGGC CACCCGGCAG
    CGAGATGGGC ATTTCAAGAA ACGCTCTCGG CCAGATCTTC GAACCAGAGC CCGAAGGAAT
    CTGTACAAAA GACAGGAGCC AGAACAAGCA GAGATCGCTA AAGACGCACA GTCTGTGACA
    CTAGACATCC CCCTCCACAA GGACGGGGAG GCTAAGACCG ACGCAGCAGG GGTGGGCAGC
    CCCCACCCAC CTGGCACGTC CTCCGCAGCA TCCAACCCAG AGAGTCCCGA ATTCCCAGTG
    GAAACTGTGG CCTCCCGGCT CCAGCCCAGT CCCAATGACC AGGCACGTGT CTCCGCGTCT
    CCAGACAGAA TTCCCAGCTT GCTGCAGGAG ACCGTGGATC AGGAACCCAA GGATCAGAAG
    AGGAAATCCT TTGAGCAGGC GACCTCTGCC TCCTTTCCCG AAAAGAAGCC CCGGCTTGAA
    GATCGTCAGT CCTTTCGTAA CACAATTGAA AGTGTTCACC CCGAAAAGCC ACAGCCCACC
    AAAGAGGAGC CCAAAGTCCC GCCCATCCGG ATTCAACTTT CACGTATCAA ACCACCCTGG
    GTGGTTAAAG GTCAGCCCAC TTACCAGATA TGTCCCCGCA TCGTCCCCAT CACGGAGTCC
    TCCTGCCGGG GCTGGACTGG TGCCAGGACC CTTGCAGACA TTAAAGCCCG TGCTCTGCAG
    GTCCGAGGGG CGAGAGGCCA CCACTGCCAT CGAGAGGCGG CCACCACTGC CATCGGAGGT
    GGGGGTGGCC CGGGTGGAGG TGGCGGCGGG GCCACCGATG AGGGAGGTGG CAGAGGCGGC
    GGCAGTGGTG ATGGTGGTGA GGCCTGTGGC CACCCTGAGC CCCGGGGGGC CCCGAGTGCC
    CCTGGAGAGT GTGCGTCAGA TCTACAGCGA ACACAACTAC TGCCGCCTTG TCCTCTAAAC
    GGGGATCACG CCCAGGCCGA AACTGCTGCG TCCAGAGCCA GGAGAGAGGA CCTGGCTTCT
    CTCAGAAAGG GGCAGAGCTG CCCACTGCAG AGGGTCCCAG ATGGCCCTAG CGGCGGGCTG
    GAAGATGCCT CCCGACTCCC CGTTGCATCC ACTCGGGATC CGCCATGCCA GGCTCTGCCC
    CCACTGCCCT CTAGAATCCC AGAACCTGAG AGGTTAGTTG AGCAGCTTGT GTCGCATCCA
    GAAGGTAGAA CTGAATGTGG GTCTGGTACC ACTGCCTGGG AAAGGGGTGA TGAGAAGCTA
    GCACCTACCA TCCCCTCAGA GAACAGTCCT GTTCGGGCTC TGGCCGGGCC TACTGGATTA
    GAAGAAGGAA CTGGCCAGGC TCCAGACAGT GGCAGTAATC CCACCATGAA GGATCCTGTG
    AACGTGACCC CCAGTTCCAT CTCCGAATCA TCCTTGGCCA GTTGCCTGCA AGACAGACCA
    TTTGATGATG AATCAGAACT TGATGACTCG GGCCCACCCA CAAGGGAAAG TTCTTCTAGA
    CAGGAAAACT TGAAAACCGA GGCTCCTGTC TCCCCTGGTG CTGCTCCTTG GAGGCCTGGC
    ATGTCAAATG ATGAGGCAGG GGGACAACCT GAACCCGACT CCAGAGAAGA GGTCCCGTCC
    ATTAAGTCCC AGGTCAGAGA GAAGTGGGAG AAAGCGGCCC CCCTCATCCC AGCATCGCCT
    GTGGGCTTGA CAGCGGAGGA GGGTCTGGAT CCCCCCGTCC ACTTGGGTTC ACTCTGGACA
    GTGCACTCTG GCTGTGTTGA CAGCAGCGGT GACTGCAGAC AGCTGGAAGG TGACAAGCCC
    AGAATCAATG GAGACTCGGA AGCTCTGAGT CCTCACAGCG AGTCCACAGA CACTGCCTCT
    GACTTCGAAG GCCACTTCTC CGAGGACAGC AGTGAGGCCG AGCCTGGTGA AACCTTGGGG
    CCGAAGAGGT CCTTGGTGGC TGAGCAGGGT GAGAAACACA GTTGGAACCA CTGTGCCTCG
    CTCTCCAAGG TGAATGGTGA CCTGAGTCTG GTCACCAGGA CAGATGGGAT GGTTGCTCCT
    CAGAGCTGGG TGTCTCGGGT ATGTGCAGTT CCCCCAAAGA TCCCAGACTC CCTGCTGCTG
    GCCAGTCCCG AGTACCAGCC GAGACCCATG TCCCTGGGCA GGCCTGCATC CTCAGTGGAG
    GCTGCTAACC CCCTTGTGAT GCAGTTGCTG CAGGGCCACT TGCCCCTCAA GAAGGTTCTC
    CCTCCAGCCC ATGGCGGCAG CAAACCCGAA CCCCCACGAC TCGCACTTAC AGCAGAGCAG
    AGCCCTGGTG GCTCCCTGGG GATGGGGTCA TTGCAAGGTC CTGGGGAGAA CAGATGTGAA
    GTTGGCAAGA GCAGTCCAGA GTCTCTGCTA CCTGAGAGCT GTGAAGCAAG TACCGGCTTT
    GCCCGGCTGG AGGCTAGCCA GGCTCCTGGG GCGCCCCTAA AGATGTCCAA GAACGCCCCG
    AGTTTAGACT CCCTATATCC AGTGACCAAT CCAGTGGCTG CCTCTGGGAA AGCAGAAGTG
    GATTTCAAAG AACAGTTATC TCCCTTCAGT TTTGAAGATC AGAAGGAAGC CCGTAACCTG
    TCCCAGTGCA GTATTTCAAC TGCTGCCCCA GGTGTGAGTC TGGGAAATCT CACTACCTCG
    AGAGCCCCTC TTTTCTCATC TCCAAATGCG GTCTCCTCGG GTCCTGATCA GGCAGGTCGG
    GCCCTGGGGG ATCAGAACAG TGCGGGAGGC CAAGGGAAGA AGCTCTTTGG CTCCAAGAAC
    GCAGCTGCAG CCCTTCAGTG CCCCCGGCTG GTGGAGCCGA CGCCACTGCC TGCGGAGGCC
    CCTCCTGCTT TTCCCAATAG GAAGTCAGGG CCAGGCAAAA CCTCTCTGTC TGGTGGGATA
    CAGACTGCCA GGGAAGACTG GGCCCCAAAG CCACCGCCTG CCTCTGTTGG CAGCATCAAG
    AGCGAAAAGA CTCTTGATGG AGGGCCTCTC AAGGTGGATG CAGAGAACAG AAAGGCAGTG
    GGGCCTGGTC CCCGGGAGCT GGTGGATCAC TTGCAAGGGA TGCCCTTTGT CCTTGATTTG
    CCCTTCTGGA AATTACCCCG GGAGCCCGGG AAGGGACTCA GTCAGCCTCT GGAGCCTTCT
    TCCATCCCCT CCCAACTCAA CATCAAGCAG GCATTTTATG GGAAGCTTTC CAAACTCCAG
    CTAAGTTCCA CCAGCTTTAA TTATTCCTCC GGCTCCCCCC CTTTTCCCAA AGGCCTGGCC
    GGAAGTGTGG TGCAGCTGAG CCACAAAGCA AACTTTGGTG CGAGCCACAG TGCGTCCCTC
    TCCTTGCAAA TGTTCACCGA CAGCAGCACA GTGGAAAGCA TCTCGCTCCA GTGTGCGTGC
    AGCCTGAAAG CCATGATCAT GTGCCAGGGC TGCGGTGCAT TCTGTCACGA TGACTGTATT
    GGACCCTCAA AGCTCTGTGT ATTGTGCCTT GTGGTGAGAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa07672
    Clone ID Related Accession (Same CDS sequence) XM_002692288.5 , XM_600364.7 , XM_002692288.4
    Accession Version XM_002692288.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4605bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product putative Polycomb group protein ASXL1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002692288.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGACTCTGG GCACCAAAAG GAAATTTGCT TGCCAGAGTT TTTACTTGTT AGAGGTACTG 
    GAAAACTATT CAGATGCTCC AATGACACCC AAACAGATTC TGCAGGTCAT AGAGGCAGAA
    GGACTAAAGG AAATGAGAAG TGGGACATCC CCTCTCGCAT GTCTTAATGC CATGCTACAT
    TCCAATTCCA GAGGAGGAGA GGGGTTATTT TATAAACTAC CTGGCCGAAT CAGCCTTTTT
    ACACTCAAGA AGGATGCCCT GCAGTGGTCT CGCAGTCCAG CTGTGGTGGA GGGAGAGGAG
    CCAGAGGACA CAGCTGATGT GGAGAGCTGT GGGTCTAATG AAGCCAGCAC TGTGAGTGGT
    GAAAATGATG TTTCTCTCGA TGAAACATCT TCTAATGCTT CCTGTTCTAC GGAATCTCAG
    AGTCGGCCTC TTTCCAACCC CAGGGACAGC TGCAGAGCTT CCTCACAGGC AAACAAGCAG
    AAGAAAAAGA CAGGGGTGAT GCTGCCTCGA GTTGTCCTGA CTCCTCTGAA GGTAAACGGG
    GCCCACGTGG AATCAGCATC AGGGTTCTCG GGCCGCCACG CCGATGGCGA GAGCGGCAGC
    CCGTCCAGCA GCAGCGGCGG CTCTCCGGCC CTGGGCAGCG CTGCTATCCG TGGCCAGGCC
    GAGCTCGCCC GGGACCCTGC CCCGCTCCTG AGAGGCTTCC GGAAGCCGGC CACAGGTCAA
    ATGAAGCGCA ACAGAGGGGA AGAGATCGAT TTTGAGACGC CTGGGTCCAT TCTCGTCAAC
    ACCAACCTCC GGGCCCTGAT CAACTCGCGG ACCTTCCATG CCCTGCCGTC CCACTTCCAG
    CAGCAGCTCC TCTTCCTCCT GCCCGAAGTA GACAGACAGG TGGGAACTGA TGGCCTGTTA
    CGTCTCAGCA GTAGTGCGCT GAACAATGAG TTTTTCACCC ACGCAGCTCA GAGCTGGCGG
    GAACGCCTGG CTGATGGTGA ATTCACTCAT GAGATGCAGG TCAGGATACG ACAAGAAATG
    GAGAAGGAAA AGAAAGTGGA ACAATGGAAA GAAAAGTTTT TCGAAGACTA CTATGGACAG
    AAGCTGGGTT TAACCAAAGA AGAGTCATTG CAGCAGAATG TGGGCCAGGA GGAGGCTGAA
    ATCAAGAGTA ACCTGCGTGT CTCAGAATCA ACACGGCCAC AGCGTGGCCC GGCCACCCGG
    CAGCGAGATG GGCATTTCAA GAAACGCTCT CGGCCAGATC TTCGAACCAG AGCCCGAAGG
    AATCTGTACA AAAGACAGGA GCCAGAACAA GCAGAGATCG CTAAAGACGC ACAGTCTGTG
    ACACTAGACA TCCCCCTCCA CAAGGACGGG GAGGCTAAGA CCGACGCAGC AGGGGTGGGC
    AGCCCCCACC CACCTGGCAC GTCCTCCGCA GCATCCAACC CAGAGAGTCC CGAATTCCCA
    GTGGAAACTG TGGCCTCCCG GCTCCAGCCC AGTCCCAATG ACCAGGCACG TGTCTCCGCG
    TCTCCAGACA GAATTCCCAG CTTGCTGCAG GAGACCGTGG ATCAGGAACC CAAGGATCAG
    AAGAGGAAAT CCTTTGAGCA GGCGACCTCT GCCTCCTTTC CCGAAAAGAA GCCCCGGCTT
    GAAGATCGTC AGTCCTTTCG TAACACAATT GAAAGTGTTC ACCCCGAAAA GCCACAGCCC
    ACCAAAGAGG AGCCCAAAGT CCCGCCCATC CGGATTCAAC TTTCACGTAT CAAACCACCC
    TGGGTGGTTA AAGGTCAGCC CACTTACCAG ATATGTCCCC GCATCGTCCC CATCACGGAG
    TCCTCCTGCC GGGGCTGGAC TGGTGCCAGG ACCCTTGCAG ACATTAAAGC CCGTGCTCTG
    CAGGTCCGAG GGGCGAGAGG CCACCACTGC CATCGAGAGG CGGCCACCAC TGCCATCGGA
    GGTGGGGGTG GCCCGGGTGG AGGTGGCGGC GGGGCCACCG ATGAGGGAGG TGGCAGAGGC
    GGCGGCAGTG GTGATGGTGG TGAGGCCTGT GGCCACCCTG AGCCCCGGGG GGCCCCGAGT
    GCCCCTGGAG AGTGTGCGTC AGATCTACAG CGAACACAAC TACTGCCGCC TTGTCCTCTA
    AACGGGGATC ACGCCCAGGC CGAAACTGCT GCGTCCAGAG CCAGGAGAGA GGACCTGGCT
    TCTCTCAGAA AGGGGCAGAG CTGCCCACTG CAGAGGGTCC CAGATGGCCC TAGCGGCGGG
    CTGGAAGATG CCTCCCGACT CCCCGTTGCA TCCACTCGGG ATCCGCCATG CCAGGCTCTG
    CCCCCACTGC CCTCTAGAAT CCCAGAACCT GAGAGGTTAG TTGAGCAGCT TGTGTCGCAT
    CCAGAAGGTA GAACTGAATG TGGGTCTGGT ACCACTGCCT GGGAAAGGGG TGATGAGAAG
    CTAGCACCTA CCATCCCCTC AGAGAACAGT CCTGTTCGGG CTCTGGCCGG GCCTACTGGA
    TTAGAAGAAG GAACTGGCCA GGCTCCAGAC AGTGGCAGTA ATCCCACCAT GAAGGATCCT
    GTGAACGTGA CCCCCAGTTC CATCTCCGAA TCATCCTTGG CCAGTTGCCT GCAAGACAGA
    CCATTTGATG ATGAATCAGA ACTTGATGAC TCGGGCCCAC CCACAAGGGA AAGTTCTTCT
    AGACAGGAAA ACTTGAAAAC CGAGGCTCCT GTCTCCCCTG GTGCTGCTCC TTGGAGGCCT
    GGCATGTCAA ATGATGAGGC AGGGGGACAA CCTGAACCCG ACTCCAGAGA AGAGGTCCCG
    TCCATTAAGT CCCAGGTCAG AGAGAAGTGG GAGAAAGCGG CCCCCCTCAT CCCAGCATCG
    CCTGTGGGCT TGACAGCGGA GGAGGGTCTG GATCCCCCCG TCCACTTGGG TTCACTCTGG
    ACAGTGCACT CTGGCTGTGT TGACAGCAGC GGTGACTGCA GACAGCTGGA AGGTGACAAG
    CCCAGAATCA ATGGAGACTC GGAAGCTCTG AGTCCTCACA GCGAGTCCAC AGACACTGCC
    TCTGACTTCG AAGGCCACTT CTCCGAGGAC AGCAGTGAGG CCGAGCCTGG TGAAACCTTG
    GGGCCGAAGA GGTCCTTGGT GGCTGAGCAG GGTGAGAAAC ACAGTTGGAA CCACTGTGCC
    TCGCTCTCCA AGGTGAATGG TGACCTGAGT CTGGTCACCA GGACAGATGG GATGGTTGCT
    CCTCAGAGCT GGGTGTCTCG GGTATGTGCA GTTCCCCCAA AGATCCCAGA CTCCCTGCTG
    CTGGCCAGTC CCGAGTACCA GCCGAGACCC ATGTCCCTGG GCAGGCCTGC ATCCTCAGTG
    GAGGCTGCTA ACCCCCTTGT GATGCAGTTG CTGCAGGGCC ACTTGCCCCT CAAGAAGGTT
    CTCCCTCCAG CCCATGGCGG CAGCAAACCC GAACCCCCAC GACTCGCACT TACAGCAGAG
    CAGAGCCCTG GTGGCTCCCT GGGGATGGGG TCATTGCAAG GTCCTGGGGA GAACAGATGT
    GAAGTTGGCA AGAGCAGTCC AGAGTCTCTG CTACCTGAGA GCTGTGAAGC AAGTACCGGC
    TTTGCCCGGC TGGAGGCTAG CCAGGCTCCT GGGGCGCCCC TAAAGATGTC CAAGAACGCC
    CCGAGTTTAG ACTCCCTATA TCCAGTGACC AATCCAGTGG CTGCCTCTGG GAAAGCAGAA
    GTGGATTTCA AAGAACAGTT ATCTCCCTTC AGTTTTGAAG ATCAGAAGGA AGCCCGTAAC
    CTGTCCCAGT GCAGTATTTC AACTGCTGCC CCAGGTGTGA GTCTGGGAAA TCTCACTACC
    TCGAGAGCCC CTCTTTTCTC ATCTCCAAAT GCGGTCTCCT CGGGTCCTGA TCAGGCAGGT
    CGGGCCCTGG GGGATCAGAA CAGTGCGGGA GGCCAAGGGA AGAAGCTCTT TGGCTCCAAG
    AACGCAGCTG CAGCCCTTCA GTGCCCCCGG CTGGTGGAGC CGACGCCACT GCCTGCGGAG
    GCCCCTCCTG CTTTTCCCAA TAGGAAGTCA GGGCCAGGCA AAACCTCTCT GTCTGGTGGG
    ATACAGACTG CCAGGGAAGA CTGGGCCCCA AAGCCACCGC CTGCCTCTGT TGGCAGCATC
    AAGAGCGAAA AGACTCTTGA TGGAGGGCCT CTCAAGGTGG ATGCAGAGAA CAGAAAGGCA
    GTGGGGCCTG GTCCCCGGGA GCTGGTGGAT CACTTGCAAG GGATGCCCTT TGTCCTTGAT
    TTGCCCTTCT GGAAATTACC CCGGGAGCCC GGGAAGGGAC TCAGTCAGCC TCTGGAGCCT
    TCTTCCATCC CCTCCCAACT CAACATCAAG CAGGCATTTT ATGGGAAGCT TTCCAAACTC
    CAGCTAAGTT CCACCAGCTT TAATTATTCC TCCGGCTCCC CCCCTTTTCC CAAAGGCCTG
    GCCGGAAGTG TGGTGCAGCT GAGCCACAAA GCAAACTTTG GTGCGAGCCA CAGTGCGTCC
    CTCTCCTTGC AAATGTTCAC CGACAGCAGC ACAGTGGAAA GCATCTCGCT CCAGTGTGCG
    TGCAGCCTGA AAGCCATGAT CATGTGCCAG GGCTGCGGTG CATTCTGTCA CGATGACTGT
    ATTGGACCCT CAAAGCTCTG TGTATTGTGC CTTGTGGTGA GATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002692334.2
    CDS241..4845
    Translation

    Target ORF information:

    RefSeq Version XM_002692288.5
    Organism Bos taurus(cattle)
    Definition Bos taurus additional sex combs like 1, transcriptional regulator (ASXL1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002692288.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGACTCTGG GCACCAAAAG GAAATTTGCT TGCCAGAGTT TTTACTTGTT AGAGGTACTG 
    GAAAACTATT CAGATGCTCC AATGACACCC AAACAGATTC TGCAGGTCAT AGAGGCAGAA
    GGACTAAAGG AAATGAGAAG TGGGACATCC CCTCTCGCAT GTCTTAATGC CATGCTACAT
    TCCAATTCCA GAGGAGGAGA GGGGTTATTT TATAAACTAC CTGGCCGAAT CAGCCTTTTT
    ACACTCAAGA AGGATGCCCT GCAGTGGTCT CGCAGTCCAG CTGTGGTGGA GGGAGAGGAG
    CCAGAGGACA CAGCTGATGT GGAGAGCTGT GGGTCTAATG AAGCCAGCAC TGTGAGTGGT
    GAAAATGATG TTTCTCTCGA TGAAACATCT TCTAATGCTT CCTGTTCTAC GGAATCTCAG
    AGTCGGCCTC TTTCCAACCC CAGGGACAGC TGCAGAGCTT CCTCACAGGC AAACAAGCAG
    AAGAAAAAGA CAGGGGTGAT GCTGCCTCGA GTTGTCCTGA CTCCTCTGAA GGTAAACGGG
    GCCCACGTGG AATCAGCATC AGGGTTCTCG GGCCGCCACG CCGATGGCGA GAGCGGCAGC
    CCGTCCAGCA GCAGCGGCGG CTCTCCGGCC CTGGGCAGCG CTGCTATCCG TGGCCAGGCC
    GAGCTCGCCC GGGACCCTGC CCCGCTCCTG AGAGGCTTCC GGAAGCCGGC CACAGGTCAA
    ATGAAGCGCA ACAGAGGGGA AGAGATCGAT TTTGAGACGC CTGGGTCCAT TCTCGTCAAC
    ACCAACCTCC GGGCCCTGAT CAACTCGCGG ACCTTCCATG CCCTGCCGTC CCACTTCCAG
    CAGCAGCTCC TCTTCCTCCT GCCCGAAGTA GACAGACAGG TGGGAACTGA TGGCCTGTTA
    CGTCTCAGCA GTAGTGCGCT GAACAATGAG TTTTTCACCC ACGCAGCTCA GAGCTGGCGG
    GAACGCCTGG CTGATGGTGA ATTCACTCAT GAGATGCAGG TCAGGATACG ACAAGAAATG
    GAGAAGGAAA AGAAAGTGGA ACAATGGAAA GAAAAGTTTT TCGAAGACTA CTATGGACAG
    AAGCTGGGTT TAACCAAAGA AGAGTCATTG CAGCAGAATG TGGGCCAGGA GGAGGCTGAA
    ATCAAGAGTA ACCTGCGTGT CTCAGAATCA ACACGGCCAC AGCGTGGCCC GGCCACCCGG
    CAGCGAGATG GGCATTTCAA GAAACGCTCT CGGCCAGATC TTCGAACCAG AGCCCGAAGG
    AATCTGTACA AAAGACAGGA GCCAGAACAA GCAGAGATCG CTAAAGACGC ACAGTCTGTG
    ACACTAGACA TCCCCCTCCA CAAGGACGGG GAGGCTAAGA CCGACGCAGC AGGGGTGGGC
    AGCCCCCACC CACCTGGCAC GTCCTCCGCA GCATCCAACC CAGAGAGTCC CGAATTCCCA
    GTGGAAACTG TGGCCTCCCG GCTCCAGCCC AGTCCCAATG ACCAGGCACG TGTCTCCGCG
    TCTCCAGACA GAATTCCCAG CTTGCTGCAG GAGACCGTGG ATCAGGAACC CAAGGATCAG
    AAGAGGAAAT CCTTTGAGCA GGCGACCTCT GCCTCCTTTC CCGAAAAGAA GCCCCGGCTT
    GAAGATCGTC AGTCCTTTCG TAACACAATT GAAAGTGTTC ACCCCGAAAA GCCACAGCCC
    ACCAAAGAGG AGCCCAAAGT CCCGCCCATC CGGATTCAAC TTTCACGTAT CAAACCACCC
    TGGGTGGTTA AAGGTCAGCC CACTTACCAG ATATGTCCCC GCATCGTCCC CATCACGGAG
    TCCTCCTGCC GGGGCTGGAC TGGTGCCAGG ACCCTTGCAG ACATTAAAGC CCGTGCTCTG
    CAGGTCCGAG GGGCGAGAGG CCACCACTGC CATCGAGAGG CGGCCACCAC TGCCATCGGA
    GGTGGGGGTG GCCCGGGTGG AGGTGGCGGC GGGGCCACCG ATGAGGGAGG TGGCAGAGGC
    GGCGGCAGTG GTGATGGTGG TGAGGCCTGT GGCCACCCTG AGCCCCGGGG GGCCCCGAGT
    GCCCCTGGAG AGTGTGCGTC AGATCTACAG CGAACACAAC TACTGCCGCC TTGTCCTCTA
    AACGGGGATC ACGCCCAGGC CGAAACTGCT GCGTCCAGAG CCAGGAGAGA GGACCTGGCT
    TCTCTCAGAA AGGGGCAGAG CTGCCCACTG CAGAGGGTCC CAGATGGCCC TAGCGGCGGG
    CTGGAAGATG CCTCCCGACT CCCCGTTGCA TCCACTCGGG ATCCGCCATG CCAGGCTCTG
    CCCCCACTGC CCTCTAGAAT CCCAGAACCT GAGAGGTTAG TTGAGCAGCT TGTGTCGCAT
    CCAGAAGGTA GAACTGAATG TGGGTCTGGT ACCACTGCCT GGGAAAGGGG TGATGAGAAG
    CTAGCACCTA CCATCCCCTC AGAGAACAGT CCTGTTCGGG CTCTGGCCGG GCCTACTGGA
    TTAGAAGAAG GAACTGGCCA GGCTCCAGAC AGTGGCAGTA ATCCCACCAT GAAGGATCCT
    GTGAACGTGA CCCCCAGTTC CATCTCCGAA TCATCCTTGG CCAGTTGCCT GCAAGACAGA
    CCATTTGATG ATGAATCAGA ACTTGATGAC TCGGGCCCAC CCACAAGGGA AAGTTCTTCT
    AGACAGGAAA ACTTGAAAAC CGAGGCTCCT GTCTCCCCTG GTGCTGCTCC TTGGAGGCCT
    GGCATGTCAA ATGATGAGGC AGGGGGACAA CCTGAACCCG ACTCCAGAGA AGAGGTCCCG
    TCCATTAAGT CCCAGGTCAG AGAGAAGTGG GAGAAAGCGG CCCCCCTCAT CCCAGCATCG
    CCTGTGGGCT TGACAGCGGA GGAGGGTCTG GATCCCCCCG TCCACTTGGG TTCACTCTGG
    ACAGTGCACT CTGGCTGTGT TGACAGCAGC GGTGACTGCA GACAGCTGGA AGGTGACAAG
    CCCAGAATCA ATGGAGACTC GGAAGCTCTG AGTCCTCACA GCGAGTCCAC AGACACTGCC
    TCTGACTTCG AAGGCCACTT CTCCGAGGAC AGCAGTGAGG CCGAGCCTGG TGAAACCTTG
    GGGCCGAAGA GGTCCTTGGT GGCTGAGCAG GGTGAGAAAC ACAGTTGGAA CCACTGTGCC
    TCGCTCTCCA AGGTGAATGG TGACCTGAGT CTGGTCACCA GGACAGATGG GATGGTTGCT
    CCTCAGAGCT GGGTGTCTCG GGTATGTGCA GTTCCCCCAA AGATCCCAGA CTCCCTGCTG
    CTGGCCAGTC CCGAGTACCA GCCGAGACCC ATGTCCCTGG GCAGGCCTGC ATCCTCAGTG
    GAGGCTGCTA ACCCCCTTGT GATGCAGTTG CTGCAGGGCC ACTTGCCCCT CAAGAAGGTT
    CTCCCTCCAG CCCATGGCGG CAGCAAACCC GAACCCCCAC GACTCGCACT TACAGCAGAG
    CAGAGCCCTG GTGGCTCCCT GGGGATGGGG TCATTGCAAG GTCCTGGGGA GAACAGATGT
    GAAGTTGGCA AGAGCAGTCC AGAGTCTCTG CTACCTGAGA GCTGTGAAGC AAGTACCGGC
    TTTGCCCGGC TGGAGGCTAG CCAGGCTCCT GGGGCGCCCC TAAAGATGTC CAAGAACGCC
    CCGAGTTTAG ACTCCCTATA TCCAGTGACC AATCCAGTGG CTGCCTCTGG GAAAGCAGAA
    GTGGATTTCA AAGAACAGTT ATCTCCCTTC AGTTTTGAAG ATCAGAAGGA AGCCCGTAAC
    CTGTCCCAGT GCAGTATTTC AACTGCTGCC CCAGGTGTGA GTCTGGGAAA TCTCACTACC
    TCGAGAGCCC CTCTTTTCTC ATCTCCAAAT GCGGTCTCCT CGGGTCCTGA TCAGGCAGGT
    CGGGCCCTGG GGGATCAGAA CAGTGCGGGA GGCCAAGGGA AGAAGCTCTT TGGCTCCAAG
    AACGCAGCTG CAGCCCTTCA GTGCCCCCGG CTGGTGGAGC CGACGCCACT GCCTGCGGAG
    GCCCCTCCTG CTTTTCCCAA TAGGAAGTCA GGGCCAGGCA AAACCTCTCT GTCTGGTGGG
    ATACAGACTG CCAGGGAAGA CTGGGCCCCA AAGCCACCGC CTGCCTCTGT TGGCAGCATC
    AAGAGCGAAA AGACTCTTGA TGGAGGGCCT CTCAAGGTGG ATGCAGAGAA CAGAAAGGCA
    GTGGGGCCTG GTCCCCGGGA GCTGGTGGAT CACTTGCAAG GGATGCCCTT TGTCCTTGAT
    TTGCCCTTCT GGAAATTACC CCGGGAGCCC GGGAAGGGAC TCAGTCAGCC TCTGGAGCCT
    TCTTCCATCC CCTCCCAACT CAACATCAAG CAGGCATTTT ATGGGAAGCT TTCCAAACTC
    CAGCTAAGTT CCACCAGCTT TAATTATTCC TCCGGCTCCC CCCCTTTTCC CAAAGGCCTG
    GCCGGAAGTG TGGTGCAGCT GAGCCACAAA GCAAACTTTG GTGCGAGCCA CAGTGCGTCC
    CTCTCCTTGC AAATGTTCAC CGACAGCAGC ACAGTGGAAA GCATCTCGCT CCAGTGTGCG
    TGCAGCCTGA AAGCCATGAT CATGTGCCAG GGCTGCGGTG CATTCTGTCA CGATGACTGT
    ATTGGACCCT CAAAGCTCTG TGTATTGTGC CTTGTGGTGA GATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa07672
    Clone ID Related Accession (Same CDS sequence) XM_002692288.5 , XM_600364.7 , XM_002692288.4
    Accession Version XM_600364.7 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4605bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product putative Polycomb group protein ASXL1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646145.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_600364.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGACTCTGG GCACCAAAAG GAAATTTGCT TGCCAGAGTT TTTACTTGTT AGAGGTACTG 
    GAAAACTATT CAGATGCTCC AATGACACCC AAACAGATTC TGCAGGTCAT AGAGGCAGAA
    GGACTAAAGG AAATGAGAAG TGGGACATCC CCTCTCGCAT GTCTTAATGC CATGCTACAT
    TCCAATTCCA GAGGAGGAGA GGGGTTATTT TATAAACTAC CTGGCCGAAT CAGCCTTTTT
    ACACTCAAGA AGGATGCCCT GCAGTGGTCT CGCAGTCCAG CTGTGGTGGA GGGAGAGGAG
    CCAGAGGACA CAGCTGATGT GGAGAGCTGT GGGTCTAATG AAGCCAGCAC TGTGAGTGGT
    GAAAATGATG TTTCTCTCGA TGAAACATCT TCTAATGCTT CCTGTTCTAC GGAATCTCAG
    AGTCGGCCTC TTTCCAACCC CAGGGACAGC TGCAGAGCTT CCTCACAGGC AAACAAGCAG
    AAGAAAAAGA CAGGGGTGAT GCTGCCTCGA GTTGTCCTGA CTCCTCTGAA GGTAAACGGG
    GCCCACGTGG AATCAGCATC AGGGTTCTCG GGCCGCCACG CCGATGGCGA GAGCGGCAGC
    CCGTCCAGCA GCAGCGGCGG CTCTCCGGCC CTGGGCAGCG CTGCTATCCG TGGCCAGGCC
    GAGCTCGCCC GGGACCCTGC CCCGCTCCTG AGAGGCTTCC GGAAGCCGGC CACAGGTCAA
    ATGAAGCGCA ACAGAGGGGA AGAGATCGAT TTTGAGACGC CTGGGTCCAT TCTCGTCAAC
    ACCAACCTCC GGGCCCTGAT CAACTCGCGG ACCTTCCATG CCCTGCCGTC CCACTTCCAG
    CAGCAGCTCC TCTTCCTCCT GCCCGAAGTA GACAGACAGG TGGGAACTGA TGGCCTGTTA
    CGTCTCAGCA GTAGTGCGCT GAACAATGAG TTTTTCACCC ACGCAGCTCA GAGCTGGCGG
    GAACGCCTGG CTGATGGTGA ATTCACTCAT GAGATGCAGG TCAGGATACG ACAAGAAATG
    GAGAAGGAAA AGAAAGTGGA ACAATGGAAA GAAAAGTTTT TCGAAGACTA CTATGGACAG
    AAGCTGGGTT TAACCAAAGA AGAGTCATTG CAGCAGAATG TGGGCCAGGA GGAGGCTGAA
    ATCAAGAGTA ACCTGCGTGT CTCAGAATCA ACACGGCCAC AGCGTGGCCC GGCCACCCGG
    CAGCGAGATG GGCATTTCAA GAAACGCTCT CGGCCAGATC TTCGAACCAG AGCCCGAAGG
    AATCTGTACA AAAGACAGGA GCCAGAACAA GCAGAGATCG CTAAAGACGC ACAGTCTGTG
    ACACTAGACA TCCCCCTCCA CAAGGACGGG GAGGCTAAGA CCGACGCAGC AGGGGTGGGC
    AGCCCCCACC CACCTGGCAC GTCCTCCGCA GCATCCAACC CAGAGAGTCC CGAATTCCCA
    GTGGAAACTG TGGCCTCCCG GCTCCAGCCC AGTCCCAATG ACCAGGCACG TGTCTCCGCG
    TCTCCAGACA GAATTCCCAG CTTGCTGCAG GAGACCGTGG ATCAGGAACC CAAGGATCAG
    AAGAGGAAAT CCTTTGAGCA GGCGACCTCT GCCTCCTTTC CCGAAAAGAA GCCCCGGCTT
    GAAGATCGTC AGTCCTTTCG TAACACAATT GAAAGTGTTC ACCCCGAAAA GCCACAGCCC
    ACCAAAGAGG AGCCCAAAGT CCCGCCCATC CGGATTCAAC TTTCACGTAT CAAACCACCC
    TGGGTGGTTA AAGGTCAGCC CACTTACCAG ATATGTCCCC GCATCGTCCC CATCACGGAG
    TCCTCCTGCC GGGGCTGGAC TGGTGCCAGG ACCCTTGCAG ACATTAAAGC CCGTGCTCTG
    CAGGTCCGAG GGGCGAGAGG CCACCACTGC CATCGAGAGG CGGCCACCAC TGCCATCGGA
    GGTGGGGGTG GCCCGGGTGG AGGTGGCGGC GGGGCCACCG ATGAGGGAGG TGGCAGAGGC
    GGCGGCAGTG GTGATGGTGG TGAGGCCTGT GGCCACCCTG AGCCCCGGGG GGCCCCGAGT
    GCCCCTGGAG AGTGTGCGTC AGATCTACAG CGAACACAAC TACTGCCGCC TTGTCCTCTA
    AACGGGGATC ACGCCCAGGC CGAAACTGCT GCGTCCAGAG CCAGGAGAGA GGACCTGGCT
    TCTCTCAGAA AGGGGCAGAG CTGCCCACTG CAGAGGGTCC CAGATGGCCC TAGCGGCGGG
    CTGGAAGATG CCTCCCGACT CCCCGTTGCA TCCACTCGGG ATCCGCCATG CCAGGCTCTG
    CCCCCACTGC CCTCTAGAAT CCCAGAACCT GAGAGGTTAG TTGAGCAGCT TGTGTCGCAT
    CCAGAAGGTA GAACTGAATG TGGGTCTGGT ACCACTGCCT GGGAAAGGGG TGATGAGAAG
    CTAGCACCTA CCATCCCCTC AGAGAACAGT CCTGTTCGGG CTCTGGCCGG GCCTACTGGA
    TTAGAAGAAG GAACTGGCCA GGCTCCAGAC AGTGGCAGTA ATCCCACCAT GAAGGATCCT
    GTGAACGTGA CCCCCAGTTC CATCTCCGAA TCATCCTTGG CCAGTTGCCT GCAAGACAGA
    CCATTTGATG ATGAATCAGA ACTTGATGAC TCGGGCCCAC CCACAAGGGA AAGTTCTTCT
    AGACAGGAAA ACTTGAAAAC CGAGGCTCCT GTCTCCCCTG GTGCTGCTCC TTGGAGGCCT
    GGCATGTCAA ATGATGAGGC AGGGGGACAA CCTGAACCCG ACTCCAGAGA AGAGGTCCCG
    TCCATTAAGT CCCAGGTCAG AGAGAAGTGG GAGAAAGCGG CCCCCCTCAT CCCAGCATCG
    CCTGTGGGCT TGACAGCGGA GGAGGGTCTG GATCCCCCCG TCCACTTGGG TTCACTCTGG
    ACAGTGCACT CTGGCTGTGT TGACAGCAGC GGTGACTGCA GACAGCTGGA AGGTGACAAG
    CCCAGAATCA ATGGAGACTC GGAAGCTCTG AGTCCTCACA GCGAGTCCAC AGACACTGCC
    TCTGACTTCG AAGGCCACTT CTCCGAGGAC AGCAGTGAGG CCGAGCCTGG TGAAACCTTG
    GGGCCGAAGA GGTCCTTGGT GGCTGAGCAG GGTGAGAAAC ACAGTTGGAA CCACTGTGCC
    TCGCTCTCCA AGGTGAATGG TGACCTGAGT CTGGTCACCA GGACAGATGG GATGGTTGCT
    CCTCAGAGCT GGGTGTCTCG GGTATGTGCA GTTCCCCCAA AGATCCCAGA CTCCCTGCTG
    CTGGCCAGTC CCGAGTACCA GCCGAGACCC ATGTCCCTGG GCAGGCCTGC ATCCTCAGTG
    GAGGCTGCTA ACCCCCTTGT GATGCAGTTG CTGCAGGGCC ACTTGCCCCT CAAGAAGGTT
    CTCCCTCCAG CCCATGGCGG CAGCAAACCC GAACCCCCAC GACTCGCACT TACAGCAGAG
    CAGAGCCCTG GTGGCTCCCT GGGGATGGGG TCATTGCAAG GTCCTGGGGA GAACAGATGT
    GAAGTTGGCA AGAGCAGTCC AGAGTCTCTG CTACCTGAGA GCTGTGAAGC AAGTACCGGC
    TTTGCCCGGC TGGAGGCTAG CCAGGCTCCT GGGGCGCCCC TAAAGATGTC CAAGAACGCC
    CCGAGTTTAG ACTCCCTATA TCCAGTGACC AATCCAGTGG CTGCCTCTGG GAAAGCAGAA
    GTGGATTTCA AAGAACAGTT ATCTCCCTTC AGTTTTGAAG ATCAGAAGGA AGCCCGTAAC
    CTGTCCCAGT GCAGTATTTC AACTGCTGCC CCAGGTGTGA GTCTGGGAAA TCTCACTACC
    TCGAGAGCCC CTCTTTTCTC ATCTCCAAAT GCGGTCTCCT CGGGTCCTGA TCAGGCAGGT
    CGGGCCCTGG GGGATCAGAA CAGTGCGGGA GGCCAAGGGA AGAAGCTCTT TGGCTCCAAG
    AACGCAGCTG CAGCCCTTCA GTGCCCCCGG CTGGTGGAGC CGACGCCACT GCCTGCGGAG
    GCCCCTCCTG CTTTTCCCAA TAGGAAGTCA GGGCCAGGCA AAACCTCTCT GTCTGGTGGG
    ATACAGACTG CCAGGGAAGA CTGGGCCCCA AAGCCACCGC CTGCCTCTGT TGGCAGCATC
    AAGAGCGAAA AGACTCTTGA TGGAGGGCCT CTCAAGGTGG ATGCAGAGAA CAGAAAGGCA
    GTGGGGCCTG GTCCCCGGGA GCTGGTGGAT CACTTGCAAG GGATGCCCTT TGTCCTTGAT
    TTGCCCTTCT GGAAATTACC CCGGGAGCCC GGGAAGGGAC TCAGTCAGCC TCTGGAGCCT
    TCTTCCATCC CCTCCCAACT CAACATCAAG CAGGCATTTT ATGGGAAGCT TTCCAAACTC
    CAGCTAAGTT CCACCAGCTT TAATTATTCC TCCGGCTCCC CCCCTTTTCC CAAAGGCCTG
    GCCGGAAGTG TGGTGCAGCT GAGCCACAAA GCAAACTTTG GTGCGAGCCA CAGTGCGTCC
    CTCTCCTTGC AAATGTTCAC CGACAGCAGC ACAGTGGAAA GCATCTCGCT CCAGTGTGCG
    TGCAGCCTGA AAGCCATGAT CATGTGCCAG GGCTGCGGTG CATTCTGTCA CGATGACTGT
    ATTGGACCCT CAAAGCTCTG TGTATTGTGC CTTGTGGTGA GATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_600364.5
    CDS478..5082
    Translation

    Target ORF information:

    RefSeq Version XM_600364.7
    Organism Bos taurus(cattle)
    Definition Bos taurus additional sex combs like 1, transcriptional regulator (ASXL1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_600364.7

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGACTCTGG GCACCAAAAG GAAATTTGCT TGCCAGAGTT TTTACTTGTT AGAGGTACTG 
    GAAAACTATT CAGATGCTCC AATGACACCC AAACAGATTC TGCAGGTCAT AGAGGCAGAA
    GGACTAAAGG AAATGAGAAG TGGGACATCC CCTCTCGCAT GTCTTAATGC CATGCTACAT
    TCCAATTCCA GAGGAGGAGA GGGGTTATTT TATAAACTAC CTGGCCGAAT CAGCCTTTTT
    ACACTCAAGA AGGATGCCCT GCAGTGGTCT CGCAGTCCAG CTGTGGTGGA GGGAGAGGAG
    CCAGAGGACA CAGCTGATGT GGAGAGCTGT GGGTCTAATG AAGCCAGCAC TGTGAGTGGT
    GAAAATGATG TTTCTCTCGA TGAAACATCT TCTAATGCTT CCTGTTCTAC GGAATCTCAG
    AGTCGGCCTC TTTCCAACCC CAGGGACAGC TGCAGAGCTT CCTCACAGGC AAACAAGCAG
    AAGAAAAAGA CAGGGGTGAT GCTGCCTCGA GTTGTCCTGA CTCCTCTGAA GGTAAACGGG
    GCCCACGTGG AATCAGCATC AGGGTTCTCG GGCCGCCACG CCGATGGCGA GAGCGGCAGC
    CCGTCCAGCA GCAGCGGCGG CTCTCCGGCC CTGGGCAGCG CTGCTATCCG TGGCCAGGCC
    GAGCTCGCCC GGGACCCTGC CCCGCTCCTG AGAGGCTTCC GGAAGCCGGC CACAGGTCAA
    ATGAAGCGCA ACAGAGGGGA AGAGATCGAT TTTGAGACGC CTGGGTCCAT TCTCGTCAAC
    ACCAACCTCC GGGCCCTGAT CAACTCGCGG ACCTTCCATG CCCTGCCGTC CCACTTCCAG
    CAGCAGCTCC TCTTCCTCCT GCCCGAAGTA GACAGACAGG TGGGAACTGA TGGCCTGTTA
    CGTCTCAGCA GTAGTGCGCT GAACAATGAG TTTTTCACCC ACGCAGCTCA GAGCTGGCGG
    GAACGCCTGG CTGATGGTGA ATTCACTCAT GAGATGCAGG TCAGGATACG ACAAGAAATG
    GAGAAGGAAA AGAAAGTGGA ACAATGGAAA GAAAAGTTTT TCGAAGACTA CTATGGACAG
    AAGCTGGGTT TAACCAAAGA AGAGTCATTG CAGCAGAATG TGGGCCAGGA GGAGGCTGAA
    ATCAAGAGTA ACCTGCGTGT CTCAGAATCA ACACGGCCAC AGCGTGGCCC GGCCACCCGG
    CAGCGAGATG GGCATTTCAA GAAACGCTCT CGGCCAGATC TTCGAACCAG AGCCCGAAGG
    AATCTGTACA AAAGACAGGA GCCAGAACAA GCAGAGATCG CTAAAGACGC ACAGTCTGTG
    ACACTAGACA TCCCCCTCCA CAAGGACGGG GAGGCTAAGA CCGACGCAGC AGGGGTGGGC
    AGCCCCCACC CACCTGGCAC GTCCTCCGCA GCATCCAACC CAGAGAGTCC CGAATTCCCA
    GTGGAAACTG TGGCCTCCCG GCTCCAGCCC AGTCCCAATG ACCAGGCACG TGTCTCCGCG
    TCTCCAGACA GAATTCCCAG CTTGCTGCAG GAGACCGTGG ATCAGGAACC CAAGGATCAG
    AAGAGGAAAT CCTTTGAGCA GGCGACCTCT GCCTCCTTTC CCGAAAAGAA GCCCCGGCTT
    GAAGATCGTC AGTCCTTTCG TAACACAATT GAAAGTGTTC ACCCCGAAAA GCCACAGCCC
    ACCAAAGAGG AGCCCAAAGT CCCGCCCATC CGGATTCAAC TTTCACGTAT CAAACCACCC
    TGGGTGGTTA AAGGTCAGCC CACTTACCAG ATATGTCCCC GCATCGTCCC CATCACGGAG
    TCCTCCTGCC GGGGCTGGAC TGGTGCCAGG ACCCTTGCAG ACATTAAAGC CCGTGCTCTG
    CAGGTCCGAG GGGCGAGAGG CCACCACTGC CATCGAGAGG CGGCCACCAC TGCCATCGGA
    GGTGGGGGTG GCCCGGGTGG AGGTGGCGGC GGGGCCACCG ATGAGGGAGG TGGCAGAGGC
    GGCGGCAGTG GTGATGGTGG TGAGGCCTGT GGCCACCCTG AGCCCCGGGG GGCCCCGAGT
    GCCCCTGGAG AGTGTGCGTC AGATCTACAG CGAACACAAC TACTGCCGCC TTGTCCTCTA
    AACGGGGATC ACGCCCAGGC CGAAACTGCT GCGTCCAGAG CCAGGAGAGA GGACCTGGCT
    TCTCTCAGAA AGGGGCAGAG CTGCCCACTG CAGAGGGTCC CAGATGGCCC TAGCGGCGGG
    CTGGAAGATG CCTCCCGACT CCCCGTTGCA TCCACTCGGG ATCCGCCATG CCAGGCTCTG
    CCCCCACTGC CCTCTAGAAT CCCAGAACCT GAGAGGTTAG TTGAGCAGCT TGTGTCGCAT
    CCAGAAGGTA GAACTGAATG TGGGTCTGGT ACCACTGCCT GGGAAAGGGG TGATGAGAAG
    CTAGCACCTA CCATCCCCTC AGAGAACAGT CCTGTTCGGG CTCTGGCCGG GCCTACTGGA
    TTAGAAGAAG GAACTGGCCA GGCTCCAGAC AGTGGCAGTA ATCCCACCAT GAAGGATCCT
    GTGAACGTGA CCCCCAGTTC CATCTCCGAA TCATCCTTGG CCAGTTGCCT GCAAGACAGA
    CCATTTGATG ATGAATCAGA ACTTGATGAC TCGGGCCCAC CCACAAGGGA AAGTTCTTCT
    AGACAGGAAA ACTTGAAAAC CGAGGCTCCT GTCTCCCCTG GTGCTGCTCC TTGGAGGCCT
    GGCATGTCAA ATGATGAGGC AGGGGGACAA CCTGAACCCG ACTCCAGAGA AGAGGTCCCG
    TCCATTAAGT CCCAGGTCAG AGAGAAGTGG GAGAAAGCGG CCCCCCTCAT CCCAGCATCG
    CCTGTGGGCT TGACAGCGGA GGAGGGTCTG GATCCCCCCG TCCACTTGGG TTCACTCTGG
    ACAGTGCACT CTGGCTGTGT TGACAGCAGC GGTGACTGCA GACAGCTGGA AGGTGACAAG
    CCCAGAATCA ATGGAGACTC GGAAGCTCTG AGTCCTCACA GCGAGTCCAC AGACACTGCC
    TCTGACTTCG AAGGCCACTT CTCCGAGGAC AGCAGTGAGG CCGAGCCTGG TGAAACCTTG
    GGGCCGAAGA GGTCCTTGGT GGCTGAGCAG GGTGAGAAAC ACAGTTGGAA CCACTGTGCC
    TCGCTCTCCA AGGTGAATGG TGACCTGAGT CTGGTCACCA GGACAGATGG GATGGTTGCT
    CCTCAGAGCT GGGTGTCTCG GGTATGTGCA GTTCCCCCAA AGATCCCAGA CTCCCTGCTG
    CTGGCCAGTC CCGAGTACCA GCCGAGACCC ATGTCCCTGG GCAGGCCTGC ATCCTCAGTG
    GAGGCTGCTA ACCCCCTTGT GATGCAGTTG CTGCAGGGCC ACTTGCCCCT CAAGAAGGTT
    CTCCCTCCAG CCCATGGCGG CAGCAAACCC GAACCCCCAC GACTCGCACT TACAGCAGAG
    CAGAGCCCTG GTGGCTCCCT GGGGATGGGG TCATTGCAAG GTCCTGGGGA GAACAGATGT
    GAAGTTGGCA AGAGCAGTCC AGAGTCTCTG CTACCTGAGA GCTGTGAAGC AAGTACCGGC
    TTTGCCCGGC TGGAGGCTAG CCAGGCTCCT GGGGCGCCCC TAAAGATGTC CAAGAACGCC
    CCGAGTTTAG ACTCCCTATA TCCAGTGACC AATCCAGTGG CTGCCTCTGG GAAAGCAGAA
    GTGGATTTCA AAGAACAGTT ATCTCCCTTC AGTTTTGAAG ATCAGAAGGA AGCCCGTAAC
    CTGTCCCAGT GCAGTATTTC AACTGCTGCC CCAGGTGTGA GTCTGGGAAA TCTCACTACC
    TCGAGAGCCC CTCTTTTCTC ATCTCCAAAT GCGGTCTCCT CGGGTCCTGA TCAGGCAGGT
    CGGGCCCTGG GGGATCAGAA CAGTGCGGGA GGCCAAGGGA AGAAGCTCTT TGGCTCCAAG
    AACGCAGCTG CAGCCCTTCA GTGCCCCCGG CTGGTGGAGC CGACGCCACT GCCTGCGGAG
    GCCCCTCCTG CTTTTCCCAA TAGGAAGTCA GGGCCAGGCA AAACCTCTCT GTCTGGTGGG
    ATACAGACTG CCAGGGAAGA CTGGGCCCCA AAGCCACCGC CTGCCTCTGT TGGCAGCATC
    AAGAGCGAAA AGACTCTTGA TGGAGGGCCT CTCAAGGTGG ATGCAGAGAA CAGAAAGGCA
    GTGGGGCCTG GTCCCCGGGA GCTGGTGGAT CACTTGCAAG GGATGCCCTT TGTCCTTGAT
    TTGCCCTTCT GGAAATTACC CCGGGAGCCC GGGAAGGGAC TCAGTCAGCC TCTGGAGCCT
    TCTTCCATCC CCTCCCAACT CAACATCAAG CAGGCATTTT ATGGGAAGCT TTCCAAACTC
    CAGCTAAGTT CCACCAGCTT TAATTATTCC TCCGGCTCCC CCCCTTTTCC CAAAGGCCTG
    GCCGGAAGTG TGGTGCAGCT GAGCCACAAA GCAAACTTTG GTGCGAGCCA CAGTGCGTCC
    CTCTCCTTGC AAATGTTCAC CGACAGCAGC ACAGTGGAAA GCATCTCGCT CCAGTGTGCG
    TGCAGCCTGA AAGCCATGAT CATGTGCCAG GGCTGCGGTG CATTCTGTCA CGATGACTGT
    ATTGGACCCT CAAAGCTCTG TGTATTGTGC CTTGTGGTGA GATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa07672
    Clone ID Related Accession (Same CDS sequence) XM_002692288.5 , XM_600364.7 , XM_002692288.4
    Accession Version XM_002692288.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4605bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product putative Polycomb group protein ASXL1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104370.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002692288.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGACTCTGG GCACCAAAAG GAAATTTGCT TGCCAGAGTT TTTACTTGTT AGAGGTACTG 
    GAAAACTATT CAGATGCTCC AATGACACCC AAACAGATTC TGCAGGTCAT AGAGGCAGAA
    GGACTAAAGG AAATGAGAAG TGGGACATCC CCTCTCGCAT GTCTTAATGC CATGCTACAT
    TCCAATTCCA GAGGAGGAGA GGGGTTATTT TATAAACTAC CTGGCCGAAT CAGCCTTTTT
    ACACTCAAGA AGGATGCCCT GCAGTGGTCT CGCAGTCCAG CTGTGGTGGA GGGAGAGGAG
    CCAGAGGACA CAGCTGATGT GGAGAGCTGT GGGTCTAATG AAGCCAGCAC TGTGAGTGGT
    GAAAATGATG TTTCTCTCGA TGAAACATCT TCTAATGCTT CCTGTTCTAC GGAATCTCAG
    AGTCGGCCTC TTTCCAACCC CAGGGACAGC TGCAGAGCTT CCTCACAGGC AAACAAGCAG
    AAGAAAAAGA CAGGGGTGAT GCTGCCTCGA GTTGTCCTGA CTCCTCTGAA GGTAAACGGG
    GCCCACGTGG AATCAGCATC AGGGTTCTCG GGCCGCCACG CCGATGGCGA GAGCGGCAGC
    CCGTCCAGCA GCAGCGGCGG CTCTCCGGCC CTGGGCAGCG CTGCTATCCG TGGCCAGGCC
    GAGCTCGCCC GGGACCCTGC CCCGCTCCTG AGAGGCTTCC GGAAGCCGGC CACAGGTCAA
    ATGAAGCGCA ACAGAGGGGA AGAGATCGAT TTTGAGACGC CTGGGTCCAT TCTCGTCAAC
    ACCAACCTCC GGGCCCTGAT CAACTCGCGG ACCTTCCATG CCCTGCCGTC CCACTTCCAG
    CAGCAGCTCC TCTTCCTCCT GCCCGAAGTA GACAGACAGG TGGGAACTGA TGGCCTGTTA
    CGTCTCAGCA GTAGTGCGCT GAACAATGAG TTTTTCACCC ACGCAGCTCA GAGCTGGCGG
    GAACGCCTGG CTGATGGTGA ATTCACTCAT GAGATGCAGG TCAGGATACG ACAAGAAATG
    GAGAAGGAAA AGAAAGTGGA ACAATGGAAA GAAAAGTTTT TCGAAGACTA CTATGGACAG
    AAGCTGGGTT TAACCAAAGA AGAGTCATTG CAGCAGAATG TGGGCCAGGA GGAGGCTGAA
    ATCAAGAGTA ACCTGCGTGT CTCAGAATCA ACACGGCCAC AGCGTGGCCC GGCCACCCGG
    CAGCGAGATG GGCATTTCAA GAAACGCTCT CGGCCAGATC TTCGAACCAG AGCCCGAAGG
    AATCTGTACA AAAGACAGGA GCCAGAACAA GCAGAGATCG CTAAAGACGC ACAGTCTGTG
    ACACTAGACA TCCCCCTCCA CAAGGACGGG GAGGCTAAGA CCGACGCAGC AGGGGTGGGC
    AGCCCCCACC CACCTGGCAC GTCCTCCGCA GCATCCAACC CAGAGAGTCC CGAATTCCCA
    GTGGAAACTG TGGCCTCCCG GCTCCAGCCC AGTCCCAATG ACCAGGCACG TGTCTCCGCG
    TCTCCAGACA GAATTCCCAG CTTGCTGCAG GAGACCGTGG ATCAGGAACC CAAGGATCAG
    AAGAGGAAAT CCTTTGAGCA GGCGACCTCT GCCTCCTTTC CCGAAAAGAA GCCCCGGCTT
    GAAGATCGTC AGTCCTTTCG TAACACAATT GAAAGTGTTC ACCCCGAAAA GCCACAGCCC
    ACCAAAGAGG AGCCCAAAGT CCCGCCCATC CGGATTCAAC TTTCACGTAT CAAACCACCC
    TGGGTGGTTA AAGGTCAGCC CACTTACCAG ATATGTCCCC GCATCGTCCC CATCACGGAG
    TCCTCCTGCC GGGGCTGGAC TGGTGCCAGG ACCCTTGCAG ACATTAAAGC CCGTGCTCTG
    CAGGTCCGAG GGGCGAGAGG CCACCACTGC CATCGAGAGG CGGCCACCAC TGCCATCGGA
    GGTGGGGGTG GCCCGGGTGG AGGTGGCGGC GGGGCCACCG ATGAGGGAGG TGGCAGAGGC
    GGCGGCAGTG GTGATGGTGG TGAGGCCTGT GGCCACCCTG AGCCCCGGGG GGCCCCGAGT
    GCCCCTGGAG AGTGTGCGTC AGATCTACAG CGAACACAAC TACTGCCGCC TTGTCCTCTA
    AACGGGGATC ACGCCCAGGC CGAAACTGCT GCGTCCAGAG CCAGGAGAGA GGACCTGGCT
    TCTCTCAGAA AGGGGCAGAG CTGCCCACTG CAGAGGGTCC CAGATGGCCC TAGCGGCGGG
    CTGGAAGATG CCTCCCGACT CCCCGTTGCA TCCACTCGGG ATCCGCCATG CCAGGCTCTG
    CCCCCACTGC CCTCTAGAAT CCCAGAACCT GAGAGGTTAG TTGAGCAGCT TGTGTCGCAT
    CCAGAAGGTA GAACTGAATG TGGGTCTGGT ACCACTGCCT GGGAAAGGGG TGATGAGAAG
    CTAGCACCTA CCATCCCCTC AGAGAACAGT CCTGTTCGGG CTCTGGCCGG GCCTACTGGA
    TTAGAAGAAG GAACTGGCCA GGCTCCAGAC AGTGGCAGTA ATCCCACCAT GAAGGATCCT
    GTGAACGTGA CCCCCAGTTC CATCTCCGAA TCATCCTTGG CCAGTTGCCT GCAAGACAGA
    CCATTTGATG ATGAATCAGA ACTTGATGAC TCGGGCCCAC CCACAAGGGA AAGTTCTTCT
    AGACAGGAAA ACTTGAAAAC CGAGGCTCCT GTCTCCCCTG GTGCTGCTCC TTGGAGGCCT
    GGCATGTCAA ATGATGAGGC AGGGGGACAA CCTGAACCCG ACTCCAGAGA AGAGGTCCCG
    TCCATTAAGT CCCAGGTCAG AGAGAAGTGG GAGAAAGCGG CCCCCCTCAT CCCAGCATCG
    CCTGTGGGCT TGACAGCGGA GGAGGGTCTG GATCCCCCCG TCCACTTGGG TTCACTCTGG
    ACAGTGCACT CTGGCTGTGT TGACAGCAGC GGTGACTGCA GACAGCTGGA AGGTGACAAG
    CCCAGAATCA ATGGAGACTC GGAAGCTCTG AGTCCTCACA GCGAGTCCAC AGACACTGCC
    TCTGACTTCG AAGGCCACTT CTCCGAGGAC AGCAGTGAGG CCGAGCCTGG TGAAACCTTG
    GGGCCGAAGA GGTCCTTGGT GGCTGAGCAG GGTGAGAAAC ACAGTTGGAA CCACTGTGCC
    TCGCTCTCCA AGGTGAATGG TGACCTGAGT CTGGTCACCA GGACAGATGG GATGGTTGCT
    CCTCAGAGCT GGGTGTCTCG GGTATGTGCA GTTCCCCCAA AGATCCCAGA CTCCCTGCTG
    CTGGCCAGTC CCGAGTACCA GCCGAGACCC ATGTCCCTGG GCAGGCCTGC ATCCTCAGTG
    GAGGCTGCTA ACCCCCTTGT GATGCAGTTG CTGCAGGGCC ACTTGCCCCT CAAGAAGGTT
    CTCCCTCCAG CCCATGGCGG CAGCAAACCC GAACCCCCAC GACTCGCACT TACAGCAGAG
    CAGAGCCCTG GTGGCTCCCT GGGGATGGGG TCATTGCAAG GTCCTGGGGA GAACAGATGT
    GAAGTTGGCA AGAGCAGTCC AGAGTCTCTG CTACCTGAGA GCTGTGAAGC AAGTACCGGC
    TTTGCCCGGC TGGAGGCTAG CCAGGCTCCT GGGGCGCCCC TAAAGATGTC CAAGAACGCC
    CCGAGTTTAG ACTCCCTATA TCCAGTGACC AATCCAGTGG CTGCCTCTGG GAAAGCAGAA
    GTGGATTTCA AAGAACAGTT ATCTCCCTTC AGTTTTGAAG ATCAGAAGGA AGCCCGTAAC
    CTGTCCCAGT GCAGTATTTC AACTGCTGCC CCAGGTGTGA GTCTGGGAAA TCTCACTACC
    TCGAGAGCCC CTCTTTTCTC ATCTCCAAAT GCGGTCTCCT CGGGTCCTGA TCAGGCAGGT
    CGGGCCCTGG GGGATCAGAA CAGTGCGGGA GGCCAAGGGA AGAAGCTCTT TGGCTCCAAG
    AACGCAGCTG CAGCCCTTCA GTGCCCCCGG CTGGTGGAGC CGACGCCACT GCCTGCGGAG
    GCCCCTCCTG CTTTTCCCAA TAGGAAGTCA GGGCCAGGCA AAACCTCTCT GTCTGGTGGG
    ATACAGACTG CCAGGGAAGA CTGGGCCCCA AAGCCACCGC CTGCCTCTGT TGGCAGCATC
    AAGAGCGAAA AGACTCTTGA TGGAGGGCCT CTCAAGGTGG ATGCAGAGAA CAGAAAGGCA
    GTGGGGCCTG GTCCCCGGGA GCTGGTGGAT CACTTGCAAG GGATGCCCTT TGTCCTTGAT
    TTGCCCTTCT GGAAATTACC CCGGGAGCCC GGGAAGGGAC TCAGTCAGCC TCTGGAGCCT
    TCTTCCATCC CCTCCCAACT CAACATCAAG CAGGCATTTT ATGGGAAGCT TTCCAAACTC
    CAGCTAAGTT CCACCAGCTT TAATTATTCC TCCGGCTCCC CCCCTTTTCC CAAAGGCCTG
    GCCGGAAGTG TGGTGCAGCT GAGCCACAAA GCAAACTTTG GTGCGAGCCA CAGTGCGTCC
    CTCTCCTTGC AAATGTTCAC CGACAGCAGC ACAGTGGAAA GCATCTCGCT CCAGTGTGCG
    TGCAGCCTGA AAGCCATGAT CATGTGCCAG GGCTGCGGTG CATTCTGTCA CGATGACTGT
    ATTGGACCCT CAAAGCTCTG TGTATTGTGC CTTGTGGTGA GATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002692334.2
    CDS456..5060
    Translation

    Target ORF information:

    RefSeq Version XM_002692288.4
    Organism Bos taurus(cattle)
    Definition Bos taurus additional sex combs like 1, transcriptional regulator (ASXL1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002692288.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGACTCTGG GCACCAAAAG GAAATTTGCT TGCCAGAGTT TTTACTTGTT AGAGGTACTG 
    GAAAACTATT CAGATGCTCC AATGACACCC AAACAGATTC TGCAGGTCAT AGAGGCAGAA
    GGACTAAAGG AAATGAGAAG TGGGACATCC CCTCTCGCAT GTCTTAATGC CATGCTACAT
    TCCAATTCCA GAGGAGGAGA GGGGTTATTT TATAAACTAC CTGGCCGAAT CAGCCTTTTT
    ACACTCAAGA AGGATGCCCT GCAGTGGTCT CGCAGTCCAG CTGTGGTGGA GGGAGAGGAG
    CCAGAGGACA CAGCTGATGT GGAGAGCTGT GGGTCTAATG AAGCCAGCAC TGTGAGTGGT
    GAAAATGATG TTTCTCTCGA TGAAACATCT TCTAATGCTT CCTGTTCTAC GGAATCTCAG
    AGTCGGCCTC TTTCCAACCC CAGGGACAGC TGCAGAGCTT CCTCACAGGC AAACAAGCAG
    AAGAAAAAGA CAGGGGTGAT GCTGCCTCGA GTTGTCCTGA CTCCTCTGAA GGTAAACGGG
    GCCCACGTGG AATCAGCATC AGGGTTCTCG GGCCGCCACG CCGATGGCGA GAGCGGCAGC
    CCGTCCAGCA GCAGCGGCGG CTCTCCGGCC CTGGGCAGCG CTGCTATCCG TGGCCAGGCC
    GAGCTCGCCC GGGACCCTGC CCCGCTCCTG AGAGGCTTCC GGAAGCCGGC CACAGGTCAA
    ATGAAGCGCA ACAGAGGGGA AGAGATCGAT TTTGAGACGC CTGGGTCCAT TCTCGTCAAC
    ACCAACCTCC GGGCCCTGAT CAACTCGCGG ACCTTCCATG CCCTGCCGTC CCACTTCCAG
    CAGCAGCTCC TCTTCCTCCT GCCCGAAGTA GACAGACAGG TGGGAACTGA TGGCCTGTTA
    CGTCTCAGCA GTAGTGCGCT GAACAATGAG TTTTTCACCC ACGCAGCTCA GAGCTGGCGG
    GAACGCCTGG CTGATGGTGA ATTCACTCAT GAGATGCAGG TCAGGATACG ACAAGAAATG
    GAGAAGGAAA AGAAAGTGGA ACAATGGAAA GAAAAGTTTT TCGAAGACTA CTATGGACAG
    AAGCTGGGTT TAACCAAAGA AGAGTCATTG CAGCAGAATG TGGGCCAGGA GGAGGCTGAA
    ATCAAGAGTA ACCTGCGTGT CTCAGAATCA ACACGGCCAC AGCGTGGCCC GGCCACCCGG
    CAGCGAGATG GGCATTTCAA GAAACGCTCT CGGCCAGATC TTCGAACCAG AGCCCGAAGG
    AATCTGTACA AAAGACAGGA GCCAGAACAA GCAGAGATCG CTAAAGACGC ACAGTCTGTG
    ACACTAGACA TCCCCCTCCA CAAGGACGGG GAGGCTAAGA CCGACGCAGC AGGGGTGGGC
    AGCCCCCACC CACCTGGCAC GTCCTCCGCA GCATCCAACC CAGAGAGTCC CGAATTCCCA
    GTGGAAACTG TGGCCTCCCG GCTCCAGCCC AGTCCCAATG ACCAGGCACG TGTCTCCGCG
    TCTCCAGACA GAATTCCCAG CTTGCTGCAG GAGACCGTGG ATCAGGAACC CAAGGATCAG
    AAGAGGAAAT CCTTTGAGCA GGCGACCTCT GCCTCCTTTC CCGAAAAGAA GCCCCGGCTT
    GAAGATCGTC AGTCCTTTCG TAACACAATT GAAAGTGTTC ACCCCGAAAA GCCACAGCCC
    ACCAAAGAGG AGCCCAAAGT CCCGCCCATC CGGATTCAAC TTTCACGTAT CAAACCACCC
    TGGGTGGTTA AAGGTCAGCC CACTTACCAG ATATGTCCCC GCATCGTCCC CATCACGGAG
    TCCTCCTGCC GGGGCTGGAC TGGTGCCAGG ACCCTTGCAG ACATTAAAGC CCGTGCTCTG
    CAGGTCCGAG GGGCGAGAGG CCACCACTGC CATCGAGAGG CGGCCACCAC TGCCATCGGA
    GGTGGGGGTG GCCCGGGTGG AGGTGGCGGC GGGGCCACCG ATGAGGGAGG TGGCAGAGGC
    GGCGGCAGTG GTGATGGTGG TGAGGCCTGT GGCCACCCTG AGCCCCGGGG GGCCCCGAGT
    GCCCCTGGAG AGTGTGCGTC AGATCTACAG CGAACACAAC TACTGCCGCC TTGTCCTCTA
    AACGGGGATC ACGCCCAGGC CGAAACTGCT GCGTCCAGAG CCAGGAGAGA GGACCTGGCT
    TCTCTCAGAA AGGGGCAGAG CTGCCCACTG CAGAGGGTCC CAGATGGCCC TAGCGGCGGG
    CTGGAAGATG CCTCCCGACT CCCCGTTGCA TCCACTCGGG ATCCGCCATG CCAGGCTCTG
    CCCCCACTGC CCTCTAGAAT CCCAGAACCT GAGAGGTTAG TTGAGCAGCT TGTGTCGCAT
    CCAGAAGGTA GAACTGAATG TGGGTCTGGT ACCACTGCCT GGGAAAGGGG TGATGAGAAG
    CTAGCACCTA CCATCCCCTC AGAGAACAGT CCTGTTCGGG CTCTGGCCGG GCCTACTGGA
    TTAGAAGAAG GAACTGGCCA GGCTCCAGAC AGTGGCAGTA ATCCCACCAT GAAGGATCCT
    GTGAACGTGA CCCCCAGTTC CATCTCCGAA TCATCCTTGG CCAGTTGCCT GCAAGACAGA
    CCATTTGATG ATGAATCAGA ACTTGATGAC TCGGGCCCAC CCACAAGGGA AAGTTCTTCT
    AGACAGGAAA ACTTGAAAAC CGAGGCTCCT GTCTCCCCTG GTGCTGCTCC TTGGAGGCCT
    GGCATGTCAA ATGATGAGGC AGGGGGACAA CCTGAACCCG ACTCCAGAGA AGAGGTCCCG
    TCCATTAAGT CCCAGGTCAG AGAGAAGTGG GAGAAAGCGG CCCCCCTCAT CCCAGCATCG
    CCTGTGGGCT TGACAGCGGA GGAGGGTCTG GATCCCCCCG TCCACTTGGG TTCACTCTGG
    ACAGTGCACT CTGGCTGTGT TGACAGCAGC GGTGACTGCA GACAGCTGGA AGGTGACAAG
    CCCAGAATCA ATGGAGACTC GGAAGCTCTG AGTCCTCACA GCGAGTCCAC AGACACTGCC
    TCTGACTTCG AAGGCCACTT CTCCGAGGAC AGCAGTGAGG CCGAGCCTGG TGAAACCTTG
    GGGCCGAAGA GGTCCTTGGT GGCTGAGCAG GGTGAGAAAC ACAGTTGGAA CCACTGTGCC
    TCGCTCTCCA AGGTGAATGG TGACCTGAGT CTGGTCACCA GGACAGATGG GATGGTTGCT
    CCTCAGAGCT GGGTGTCTCG GGTATGTGCA GTTCCCCCAA AGATCCCAGA CTCCCTGCTG
    CTGGCCAGTC CCGAGTACCA GCCGAGACCC ATGTCCCTGG GCAGGCCTGC ATCCTCAGTG
    GAGGCTGCTA ACCCCCTTGT GATGCAGTTG CTGCAGGGCC ACTTGCCCCT CAAGAAGGTT
    CTCCCTCCAG CCCATGGCGG CAGCAAACCC GAACCCCCAC GACTCGCACT TACAGCAGAG
    CAGAGCCCTG GTGGCTCCCT GGGGATGGGG TCATTGCAAG GTCCTGGGGA GAACAGATGT
    GAAGTTGGCA AGAGCAGTCC AGAGTCTCTG CTACCTGAGA GCTGTGAAGC AAGTACCGGC
    TTTGCCCGGC TGGAGGCTAG CCAGGCTCCT GGGGCGCCCC TAAAGATGTC CAAGAACGCC
    CCGAGTTTAG ACTCCCTATA TCCAGTGACC AATCCAGTGG CTGCCTCTGG GAAAGCAGAA
    GTGGATTTCA AAGAACAGTT ATCTCCCTTC AGTTTTGAAG ATCAGAAGGA AGCCCGTAAC
    CTGTCCCAGT GCAGTATTTC AACTGCTGCC CCAGGTGTGA GTCTGGGAAA TCTCACTACC
    TCGAGAGCCC CTCTTTTCTC ATCTCCAAAT GCGGTCTCCT CGGGTCCTGA TCAGGCAGGT
    CGGGCCCTGG GGGATCAGAA CAGTGCGGGA GGCCAAGGGA AGAAGCTCTT TGGCTCCAAG
    AACGCAGCTG CAGCCCTTCA GTGCCCCCGG CTGGTGGAGC CGACGCCACT GCCTGCGGAG
    GCCCCTCCTG CTTTTCCCAA TAGGAAGTCA GGGCCAGGCA AAACCTCTCT GTCTGGTGGG
    ATACAGACTG CCAGGGAAGA CTGGGCCCCA AAGCCACCGC CTGCCTCTGT TGGCAGCATC
    AAGAGCGAAA AGACTCTTGA TGGAGGGCCT CTCAAGGTGG ATGCAGAGAA CAGAAAGGCA
    GTGGGGCCTG GTCCCCGGGA GCTGGTGGAT CACTTGCAAG GGATGCCCTT TGTCCTTGAT
    TTGCCCTTCT GGAAATTACC CCGGGAGCCC GGGAAGGGAC TCAGTCAGCC TCTGGAGCCT
    TCTTCCATCC CCTCCCAACT CAACATCAAG CAGGCATTTT ATGGGAAGCT TTCCAAACTC
    CAGCTAAGTT CCACCAGCTT TAATTATTCC TCCGGCTCCC CCCCTTTTCC CAAAGGCCTG
    GCCGGAAGTG TGGTGCAGCT GAGCCACAAA GCAAACTTTG GTGCGAGCCA CAGTGCGTCC
    CTCTCCTTGC AAATGTTCAC CGACAGCAGC ACAGTGGAAA GCATCTCGCT CCAGTGTGCG
    TGCAGCCTGA AAGCCATGAT CATGTGCCAG GGCTGCGGTG CATTCTGTCA CGATGACTGT
    ATTGGACCCT CAAAGCTCTG TGTATTGTGC CTTGTGGTGA GATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.