EEF2K cDNA ORF clone, Bos taurus(cattle)

The following EEF2K gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EEF2K cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa50453 XM_010819305.2
Latest version!
Bos taurus eukaryotic elongation factor 2 kinase (EEF2K), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa32973 NM_001192542.1
Latest version!
Bos taurus eukaryotic elongation factor 2 kinase (EEF2K), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa32973 XM_005224739.4
Latest version!
Bos taurus eukaryotic elongation factor 2 kinase (EEF2K), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa32974 XM_005224740.4
Latest version!
Bos taurus eukaryotic elongation factor 2 kinase (EEF2K), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
View Old Accession Versions >>
OBa32974 XM_005224740.3 Bos taurus eukaryotic elongation factor 2 kinase (EEF2K), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa50453 XM_010819305.1 Bos taurus eukaryotic elongation factor 2 kinase (EEF2K), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa32973 XM_005224739.3 Bos taurus eukaryotic elongation factor 2 kinase (EEF2K), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa50453
    Clone ID Related Accession (Same CDS sequence) XM_010819305.1 , XM_010819305.2
    Accession Version XM_010819305.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1896bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product eukaryotic elongation factor 2 kinase isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104572.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGCCTGACC CCTGGGCTGA GTTCCACCTG GAAGACGTTG CCACGGAATG TGCCACACGC 
    CACAGGTACA ACGCTGTTAC CGGCGAGTGG GTGGAGGATG AAGTCCTAAT CAAAATGGCA
    TCCCAGCCCT TCGGCCGGGG AGCAATGAGG GAGTGCTTCA GAACGAAGAA GCTCTCCAAC
    TTCCTGCACA CCCAGCAGTG GAAGGGGGCC TCCAACTACG TGGCAAAGCG CTACATCGAG
    TCCGTGGACA GGGACGTGTA CTTCGAGGAC GTGCGTCTAC AGATGGAGGC CAAGCTCTGG
    GGAGAGGAGT ATAATCGGCA CAAGCCCCCC AAGCAGGTGG ACATCATGCA GATGTGCATC
    ATAGAGCTGA AGGAGAGACC GGGCCGGCCG CTATTCCACC TGGAACACTA CATCGAGGGC
    AAGTACATCA AATATAACTC CAACTCAGGC TTCGTCCGTG ATGACAACGT GCGCCTGACG
    CCACAGGCCT TCAGCCACTT CACGTTTGAG CGTTCCGGCC ACCAGCTGAT TGTGGTGGAC
    ATCCAGGGCG TGGGTGACCT CTATACCGAC CCACAGATCC ACACTGAGAG GGGCACTGAC
    TTTGGAGATG GAAACCTAGG TGTCCGCGGG ATGGCTCTCT TCTTCCACTC ACATGCCTGC
    AACCGGATTT GCAAGAGCAT GGGCCTCACT CCCTTTGACC TGTCCCCGAG AGAGAGGGAT
    GCAGTGAATC AGAACACCAA GCTGCTGCAG TCAGCCAAGA CCATCTTAAG GGGGACAGAG
    GAAAAGTGCG GGAGCCCCCG GGTGAGGACC CTCTCAGGGA GCCGGCCCCC ACTGCTGCTT
    CGCCTCTCAG AGAACTCCGG AGATGACAAC ATGAGCGACG TGACCTTTGA CTCTCTCCCT
    TCCTCTCCGT CTTCAGCCAC ACCACATAGC CAGAAGCTGG AGCACCTCCA TTGGCCTGTG
    TTCAGCGACC TTGATAACAT GGCACCTCGA GACCAGGACC CTCTGGACAA CCACCGGGAC
    TCTGAGAACG GTGGGGACAG TGGATACCCC AGTGAGAAGC GGGGTGACCT GGATGACCCA
    GAGCCCAGAG AACATGGCCA CTCAAACGGT AACCGGAGGT ACGAGTCGGA TGAAGACAGC
    CTGGGCAGCT CCGGACGGGT CTGTGTGGAG AAGTGGAATC TCTTCAACTC CTCCCGCCTC
    CACCTGCCGA GACCTTCGGC CGTGGCCCTG GAAGTGCAGA GGCTCAACGC TCTGGACCTC
    GAGAAGAAAA TCGGGAAGTC CATTTTGGGG AAGGTCCATC TGGCCATGGT GCGCTACCAC
    GAGGCTGGCC GCTTCTGCGA GAAGGACGAG GAGTGGGACC GGGAGTCGGC GGTCTTCCAC
    CTGGAGCACG CAGCTGACCT GGGCGAGCTG GAGGCCATCG TGGGCCTGGG ACTCATGTAC
    TCGCAGCTGC CCCACCACAT CCTGGCGGAT GTCTCTCTGA AGGAGACAGA AGAGAGCAAA
    ACCAAAGGAT TTGATTACTT ACTGAAGGCA GCTGAAGCTG GGGACAGACA GTGCATGATT
    CTGGTGGCAC GAGCCTTTGA CACTGGCCAG AACCTCAGCC CTGACAGGTC CCAGAGCTGG
    CCAGAGGCTC TCCACTGGTA CAACACTGCC CTGGAGACAA CTGACTGTGA TGAGGGTGGG
    GAGTATGATG GGATGCAAGA CGAACCCCGG TACATGCTGC TGGCCAGGGA GGCGGAGATG
    CTGTTCACAG GCGGCTGCGG GCTGGAGAAG GACCCACAGA GATCAGGCGA TTTGTACACC
    CAGGCAGCCG AGGCAGCGAT GGAAGCCATG AAGGGCCGGC TGGCCAACCA GTACTACGAG
    AAAGCAGAGG AGGCCTGGGC CCAGATGGAG GAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010817607.1
    CDS338..2233
    Translation

    Target ORF information:

    RefSeq Version XM_010819305.1
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic elongation factor 2 kinase (EEF2K), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010819305.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGCCTGACC CCTGGGCTGA GTTCCACCTG GAAGACGTTG CCACGGAATG TGCCACACGC 
    CACAGGTACA ACGCTGTTAC CGGCGAGTGG GTGGAGGATG AAGTCCTAAT CAAAATGGCA
    TCCCAGCCCT TCGGCCGGGG AGCAATGAGG GAGTGCTTCA GAACGAAGAA GCTCTCCAAC
    TTCCTGCACA CCCAGCAGTG GAAGGGGGCC TCCAACTACG TGGCAAAGCG CTACATCGAG
    TCCGTGGACA GGGACGTGTA CTTCGAGGAC GTGCGTCTAC AGATGGAGGC CAAGCTCTGG
    GGAGAGGAGT ATAATCGGCA CAAGCCCCCC AAGCAGGTGG ACATCATGCA GATGTGCATC
    ATAGAGCTGA AGGAGAGACC GGGCCGGCCG CTATTCCACC TGGAACACTA CATCGAGGGC
    AAGTACATCA AATATAACTC CAACTCAGGC TTCGTCCGTG ATGACAACGT GCGCCTGACG
    CCACAGGCCT TCAGCCACTT CACGTTTGAG CGTTCCGGCC ACCAGCTGAT TGTGGTGGAC
    ATCCAGGGCG TGGGTGACCT CTATACCGAC CCACAGATCC ACACTGAGAG GGGCACTGAC
    TTTGGAGATG GAAACCTAGG TGTCCGCGGG ATGGCTCTCT TCTTCCACTC ACATGCCTGC
    AACCGGATTT GCAAGAGCAT GGGCCTCACT CCCTTTGACC TGTCCCCGAG AGAGAGGGAT
    GCAGTGAATC AGAACACCAA GCTGCTGCAG TCAGCCAAGA CCATCTTAAG GGGGACAGAG
    GAAAAGTGCG GGAGCCCCCG GGTGAGGACC CTCTCAGGGA GCCGGCCCCC ACTGCTGCTT
    CGCCTCTCAG AGAACTCCGG AGATGACAAC ATGAGCGACG TGACCTTTGA CTCTCTCCCT
    TCCTCTCCGT CTTCAGCCAC ACCACATAGC CAGAAGCTGG AGCACCTCCA TTGGCCTGTG
    TTCAGCGACC TTGATAACAT GGCACCTCGA GACCAGGACC CTCTGGACAA CCACCGGGAC
    TCTGAGAACG GTGGGGACAG TGGATACCCC AGTGAGAAGC GGGGTGACCT GGATGACCCA
    GAGCCCAGAG AACATGGCCA CTCAAACGGT AACCGGAGGT ACGAGTCGGA TGAAGACAGC
    CTGGGCAGCT CCGGACGGGT CTGTGTGGAG AAGTGGAATC TCTTCAACTC CTCCCGCCTC
    CACCTGCCGA GACCTTCGGC CGTGGCCCTG GAAGTGCAGA GGCTCAACGC TCTGGACCTC
    GAGAAGAAAA TCGGGAAGTC CATTTTGGGG AAGGTCCATC TGGCCATGGT GCGCTACCAC
    GAGGCTGGCC GCTTCTGCGA GAAGGACGAG GAGTGGGACC GGGAGTCGGC GGTCTTCCAC
    CTGGAGCACG CAGCTGACCT GGGCGAGCTG GAGGCCATCG TGGGCCTGGG ACTCATGTAC
    TCGCAGCTGC CCCACCACAT CCTGGCGGAT GTCTCTCTGA AGGAGACAGA AGAGAGCAAA
    ACCAAAGGAT TTGATTACTT ACTGAAGGCA GCTGAAGCTG GGGACAGACA GTGCATGATT
    CTGGTGGCAC GAGCCTTTGA CACTGGCCAG AACCTCAGCC CTGACAGGTC CCAGAGCTGG
    CCAGAGGCTC TCCACTGGTA CAACACTGCC CTGGAGACAA CTGACTGTGA TGAGGGTGGG
    GAGTATGATG GGATGCAAGA CGAACCCCGG TACATGCTGC TGGCCAGGGA GGCGGAGATG
    CTGTTCACAG GCGGCTGCGG GCTGGAGAAG GACCCACAGA GATCAGGCGA TTTGTACACC
    CAGGCAGCCG AGGCAGCGAT GGAAGCCATG AAGGGCCGGC TGGCCAACCA GTACTACGAG
    AAAGCAGAGG AGGCCTGGGC CCAGATGGAG GAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50453
    Clone ID Related Accession (Same CDS sequence) XM_010819305.1 , XM_010819305.2
    Accession Version XM_010819305.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1896bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product eukaryotic elongation factor 2 kinase isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010819305.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGCCTGACC CCTGGGCTGA GTTCCACCTG GAAGACGTTG CCACGGAATG TGCCACACGC 
    CACAGGTACA ACGCTGTTAC CGGCGAGTGG GTGGAGGATG AAGTCCTAAT CAAAATGGCA
    TCCCAGCCCT TCGGCCGGGG AGCAATGAGG GAGTGCTTCA GAACGAAGAA GCTCTCCAAC
    TTCCTGCACA CCCAGCAGTG GAAGGGGGCC TCCAACTACG TGGCAAAGCG CTACATCGAG
    TCCGTGGACA GGGACGTGTA CTTCGAGGAC GTGCGTCTAC AGATGGAGGC CAAGCTCTGG
    GGAGAGGAGT ATAATCGGCA CAAGCCCCCC AAGCAGGTGG ACATCATGCA GATGTGCATC
    ATAGAGCTGA AGGAGAGACC GGGCCGGCCG CTATTCCACC TGGAACACTA CATCGAGGGC
    AAGTACATCA AATATAACTC CAACTCAGGC TTCGTCCGTG ATGACAACGT GCGCCTGACG
    CCACAGGCCT TCAGCCACTT CACGTTTGAG CGTTCCGGCC ACCAGCTGAT TGTGGTGGAC
    ATCCAGGGCG TGGGTGACCT CTATACCGAC CCACAGATCC ACACTGAGAG GGGCACTGAC
    TTTGGAGATG GAAACCTAGG TGTCCGCGGG ATGGCTCTCT TCTTCCACTC ACATGCCTGC
    AACCGGATTT GCAAGAGCAT GGGCCTCACT CCCTTTGACC TGTCCCCGAG AGAGAGGGAT
    GCAGTGAATC AGAACACCAA GCTGCTGCAG TCAGCCAAGA CCATCTTAAG GGGGACAGAG
    GAAAAGTGCG GGAGCCCCCG GGTGAGGACC CTCTCAGGGA GCCGGCCCCC ACTGCTGCTT
    CGCCTCTCAG AGAACTCCGG AGATGACAAC ATGAGCGACG TGACCTTTGA CTCTCTCCCT
    TCCTCTCCGT CTTCAGCCAC ACCACATAGC CAGAAGCTGG AGCACCTCCA TTGGCCTGTG
    TTCAGCGACC TTGATAACAT GGCACCTCGA GACCAGGACC CTCTGGACAA CCACCGGGAC
    TCTGAGAACG GTGGGGACAG TGGATACCCC AGTGAGAAGC GGGGTGACCT GGATGACCCA
    GAGCCCAGAG AACATGGCCA CTCAAACGGT AACCGGAGGT ACGAGTCGGA TGAAGACAGC
    CTGGGCAGCT CCGGACGGGT CTGTGTGGAG AAGTGGAATC TCTTCAACTC CTCCCGCCTC
    CACCTGCCGA GACCTTCGGC CGTGGCCCTG GAAGTGCAGA GGCTCAACGC TCTGGACCTC
    GAGAAGAAAA TCGGGAAGTC CATTTTGGGG AAGGTCCATC TGGCCATGGT GCGCTACCAC
    GAGGCTGGCC GCTTCTGCGA GAAGGACGAG GAGTGGGACC GGGAGTCGGC GGTCTTCCAC
    CTGGAGCACG CAGCTGACCT GGGCGAGCTG GAGGCCATCG TGGGCCTGGG ACTCATGTAC
    TCGCAGCTGC CCCACCACAT CCTGGCGGAT GTCTCTCTGA AGGAGACAGA AGAGAGCAAA
    ACCAAAGGAT TTGATTACTT ACTGAAGGCA GCTGAAGCTG GGGACAGACA GTGCATGATT
    CTGGTGGCAC GAGCCTTTGA CACTGGCCAG AACCTCAGCC CTGACAGGTC CCAGAGCTGG
    CCAGAGGCTC TCCACTGGTA CAACACTGCC CTGGAGACAA CTGACTGTGA TGAGGGTGGG
    GAGTATGATG GGATGCAAGA CGAACCCCGG TACATGCTGC TGGCCAGGGA GGCGGAGATG
    CTGTTCACAG GCGGCTGCGG GCTGGAGAAG GACCCACAGA GATCAGGCGA TTTGTACACC
    CAGGCAGCCG AGGCAGCGAT GGAAGCCATG AAGGGCCGGC TGGCCAACCA GTACTACGAG
    AAAGCAGAGG AGGCCTGGGC CCAGATGGAG GAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010817607.1
    CDS359..2254
    Translation

    Target ORF information:

    RefSeq Version XM_010819305.2
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic elongation factor 2 kinase (EEF2K), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010819305.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGCCTGACC CCTGGGCTGA GTTCCACCTG GAAGACGTTG CCACGGAATG TGCCACACGC 
    CACAGGTACA ACGCTGTTAC CGGCGAGTGG GTGGAGGATG AAGTCCTAAT CAAAATGGCA
    TCCCAGCCCT TCGGCCGGGG AGCAATGAGG GAGTGCTTCA GAACGAAGAA GCTCTCCAAC
    TTCCTGCACA CCCAGCAGTG GAAGGGGGCC TCCAACTACG TGGCAAAGCG CTACATCGAG
    TCCGTGGACA GGGACGTGTA CTTCGAGGAC GTGCGTCTAC AGATGGAGGC CAAGCTCTGG
    GGAGAGGAGT ATAATCGGCA CAAGCCCCCC AAGCAGGTGG ACATCATGCA GATGTGCATC
    ATAGAGCTGA AGGAGAGACC GGGCCGGCCG CTATTCCACC TGGAACACTA CATCGAGGGC
    AAGTACATCA AATATAACTC CAACTCAGGC TTCGTCCGTG ATGACAACGT GCGCCTGACG
    CCACAGGCCT TCAGCCACTT CACGTTTGAG CGTTCCGGCC ACCAGCTGAT TGTGGTGGAC
    ATCCAGGGCG TGGGTGACCT CTATACCGAC CCACAGATCC ACACTGAGAG GGGCACTGAC
    TTTGGAGATG GAAACCTAGG TGTCCGCGGG ATGGCTCTCT TCTTCCACTC ACATGCCTGC
    AACCGGATTT GCAAGAGCAT GGGCCTCACT CCCTTTGACC TGTCCCCGAG AGAGAGGGAT
    GCAGTGAATC AGAACACCAA GCTGCTGCAG TCAGCCAAGA CCATCTTAAG GGGGACAGAG
    GAAAAGTGCG GGAGCCCCCG GGTGAGGACC CTCTCAGGGA GCCGGCCCCC ACTGCTGCTT
    CGCCTCTCAG AGAACTCCGG AGATGACAAC ATGAGCGACG TGACCTTTGA CTCTCTCCCT
    TCCTCTCCGT CTTCAGCCAC ACCACATAGC CAGAAGCTGG AGCACCTCCA TTGGCCTGTG
    TTCAGCGACC TTGATAACAT GGCACCTCGA GACCAGGACC CTCTGGACAA CCACCGGGAC
    TCTGAGAACG GTGGGGACAG TGGATACCCC AGTGAGAAGC GGGGTGACCT GGATGACCCA
    GAGCCCAGAG AACATGGCCA CTCAAACGGT AACCGGAGGT ACGAGTCGGA TGAAGACAGC
    CTGGGCAGCT CCGGACGGGT CTGTGTGGAG AAGTGGAATC TCTTCAACTC CTCCCGCCTC
    CACCTGCCGA GACCTTCGGC CGTGGCCCTG GAAGTGCAGA GGCTCAACGC TCTGGACCTC
    GAGAAGAAAA TCGGGAAGTC CATTTTGGGG AAGGTCCATC TGGCCATGGT GCGCTACCAC
    GAGGCTGGCC GCTTCTGCGA GAAGGACGAG GAGTGGGACC GGGAGTCGGC GGTCTTCCAC
    CTGGAGCACG CAGCTGACCT GGGCGAGCTG GAGGCCATCG TGGGCCTGGG ACTCATGTAC
    TCGCAGCTGC CCCACCACAT CCTGGCGGAT GTCTCTCTGA AGGAGACAGA AGAGAGCAAA
    ACCAAAGGAT TTGATTACTT ACTGAAGGCA GCTGAAGCTG GGGACAGACA GTGCATGATT
    CTGGTGGCAC GAGCCTTTGA CACTGGCCAG AACCTCAGCC CTGACAGGTC CCAGAGCTGG
    CCAGAGGCTC TCCACTGGTA CAACACTGCC CTGGAGACAA CTGACTGTGA TGAGGGTGGG
    GAGTATGATG GGATGCAAGA CGAACCCCGG TACATGCTGC TGGCCAGGGA GGCGGAGATG
    CTGTTCACAG GCGGCTGCGG GCTGGAGAAG GACCCACAGA GATCAGGCGA TTTGTACACC
    CAGGCAGCCG AGGCAGCGAT GGAAGCCATG AAGGGCCGGC TGGCCAACCA GTACTACGAG
    AAAGCAGAGG AGGCCTGGGC CCAGATGGAG GAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa32973
    Clone ID Related Accession (Same CDS sequence) NM_001192542.1 , XM_005224739.3 , XM_005224739.4
    Accession Version NM_001192542.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2172bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-28
    Organism Bos taurus(cattle)
    Product eukaryotic elongation factor 2 kinase
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000025.1. On Jul 17, 2010 this sequence version replaced XM_599997.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR6764281.3876.1, SRR5721697.2246.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCGAGG AAGACCTCAT CTTCCGCCTG GAAGGCGTGG ATGGCCGCCC AGCCTCCCGG 
    GCTGGTGATT CAGACACAGA CAGCGACGAT GATGAGGAGG GTTACTTCAT CTGCTCCATC
    ACCGATGACC CCGGGGCACA CCAGAATGTC AACTTCAAGG TTAATAACTG CCACAGCAAC
    CTAGCAAAAA GCGAGCGCTA CGGCTCCAGC GGGTCCCCGG CTGGCTCCTT CCATTTCAAG
    GAAGCTTGGA AGCATGCAAT CGAGAAAGCC AAGCACATGC CTGACCCCTG GGCTGAGTTC
    CACCTGGAAG ACGTTGCCAC GGAATGTGCC ACACGCCACA GGTACAACGC TGTTACCGGC
    GAGTGGGTGG AGGATGAAGT CCTAATCAAA ATGGCATCCC AGCCCTTCGG CCGGGGAGCA
    ATGAGGGAGT GCTTCAGAAC GAAGAAGCTC TCCAACTTCC TGCACACCCA GCAGTGGAAG
    GGGGCCTCCA ACTACGTGGC AAAGCGCTAC ATCGAGTCCG TGGACAGGGA CGTGTACTTC
    GAGGACGTGC GTCTACAGAT GGAGGCCAAG CTCTGGGGAG AGGAGTATAA TCGGCACAAG
    CCCCCCAAGC AGGTGGACAT CATGCAGATG TGCATCATAG AGCTGAAGGA GAGACCGGGC
    CGGCCGCTAT TCCACCTGGA ACACTACATC GAGGGCAAGT ACATCAAATA TAACTCCAAC
    TCAGGCTTCG TCCGTGATGA CAACGTGCGC CTGACGCCAC AGGCCTTCAG CCACTTCACG
    TTTGAGCGTT CCGGCCACCA GCTGATTGTG GTGGACATCC AGGGCGTGGG TGACCTCTAT
    ACCGACCCAC AGATCCACAC TGAGAGGGGC ACTGACTTTG GAGATGGAAA CCTAGGTGTC
    CGCGGGATGG CTCTCTTCTT CCACTCACAT GCCTGCAACC GGATTTGCAA GAGCATGGGC
    CTCACTCCCT TTGACCTGTC CCCGAGAGAG AGGGATGCAG TGAATCAGAA CACCAAGCTG
    CTGCAGTCAG CCAAGACCAT CTTAAGGGGG ACAGAGGAAA AGTGCGGGAG CCCCCGGGTG
    AGGACCCTCT CAGGGAGCCG GCCCCCACTG CTGCTTCGCC TCTCAGAGAA CTCCGGAGAT
    GACAACATGA GCGACGTGAC CTTTGACTCT CTCCCTTCCT CTCCGTCTTC AGCCACACCA
    CATAGCCAGA AGCTGGAGCA CCTCCATTGG CCTGTGTTCA GCGACCTTGA TAACATGGCA
    CCTCGAGACC AGGACCCTCT GGACAACCAC CGGGACTCTG AGAACGGTGG GGACAGTGGA
    TACCCCAGTG AGAAGCGGGG TGACCTGGAT GACCCAGAGC CCAGAGAACA TGGCCACTCA
    AACGGTAACC GGAGGTACGA GTCGGATGAA GACAGCCTGG GCAGCTCCGG ACGGGTCTGT
    GTGGAGAAGT GGAATCTCTT CAACTCCTCC CGCCTCCACC TGCCGAGACC TTCGGCCGTG
    GCCCTGGAAG TGCAGAGGCT CAACGCTCTG GACCTCGAGA AGAAAATCGG GAAGTCCATT
    TTGGGGAAGG TCCATCTGGC CATGGTGCGC TACCACGAGG CTGGCCGCTT CTGCGAGAAG
    GACGAGGAGT GGGACCGGGA GTCGGCGGTC TTCCACCTGG AGCACGCAGC TGACCTGGGC
    GAGCTGGAGG CCATCGTGGG CCTGGGACTC ATGTACTCGC AGCTGCCCCA CCACATCCTG
    GCGGATGTCT CTCTGAAGGA GACAGAAGAG AGCAAAACCA AAGGATTTGA TTACTTACTG
    AAGGCAGCTG AAGCTGGGGA CAGACAGTGC ATGATTCTGG TGGCACGAGC CTTTGACACT
    GGCCAGAACC TCAGCCCTGA CAGGTCCCAG AGCTGGCCAG AGGCTCTCCA CTGGTACAAC
    ACTGCCCTGG AGACAACTGA CTGTGATGAG GGTGGGGAGT ATGATGGGAT GCAAGACGAA
    CCCCGGTACA TGCTGCTGGC CAGGGAGGCG GAGATGCTGT TCACAGGCGG CTGCGGGCTG
    GAGAAGGACC CACAGAGATC AGGCGATTTG TACACCCAGG CAGCCGAGGC AGCGATGGAA
    GCCATGAAGG GCCGGCTGGC CAACCAGTAC TACGAGAAAG CAGAGGAGGC CTGGGCCCAG
    ATGGAGGAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001179471.1
    CDS149..2320
    Translation

    Target ORF information:

    RefSeq Version NM_001192542.1
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic elongation factor 2 kinase (EEF2K), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001192542.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCGAGG AAGACCTCAT CTTCCGCCTG GAAGGCGTGG ATGGCCGCCC AGCCTCCCGG 
    GCTGGTGATT CAGACACAGA CAGCGACGAT GATGAGGAGG GTTACTTCAT CTGCTCCATC
    ACCGATGACC CCGGGGCACA CCAGAATGTC AACTTCAAGG TTAATAACTG CCACAGCAAC
    CTAGCAAAAA GCGAGCGCTA CGGCTCCAGC GGGTCCCCGG CTGGCTCCTT CCATTTCAAG
    GAAGCTTGGA AGCATGCAAT CGAGAAAGCC AAGCACATGC CTGACCCCTG GGCTGAGTTC
    CACCTGGAAG ACGTTGCCAC GGAATGTGCC ACACGCCACA GGTACAACGC TGTTACCGGC
    GAGTGGGTGG AGGATGAAGT CCTAATCAAA ATGGCATCCC AGCCCTTCGG CCGGGGAGCA
    ATGAGGGAGT GCTTCAGAAC GAAGAAGCTC TCCAACTTCC TGCACACCCA GCAGTGGAAG
    GGGGCCTCCA ACTACGTGGC AAAGCGCTAC ATCGAGTCCG TGGACAGGGA CGTGTACTTC
    GAGGACGTGC GTCTACAGAT GGAGGCCAAG CTCTGGGGAG AGGAGTATAA TCGGCACAAG
    CCCCCCAAGC AGGTGGACAT CATGCAGATG TGCATCATAG AGCTGAAGGA GAGACCGGGC
    CGGCCGCTAT TCCACCTGGA ACACTACATC GAGGGCAAGT ACATCAAATA TAACTCCAAC
    TCAGGCTTCG TCCGTGATGA CAACGTGCGC CTGACGCCAC AGGCCTTCAG CCACTTCACG
    TTTGAGCGTT CCGGCCACCA GCTGATTGTG GTGGACATCC AGGGCGTGGG TGACCTCTAT
    ACCGACCCAC AGATCCACAC TGAGAGGGGC ACTGACTTTG GAGATGGAAA CCTAGGTGTC
    CGCGGGATGG CTCTCTTCTT CCACTCACAT GCCTGCAACC GGATTTGCAA GAGCATGGGC
    CTCACTCCCT TTGACCTGTC CCCGAGAGAG AGGGATGCAG TGAATCAGAA CACCAAGCTG
    CTGCAGTCAG CCAAGACCAT CTTAAGGGGG ACAGAGGAAA AGTGCGGGAG CCCCCGGGTG
    AGGACCCTCT CAGGGAGCCG GCCCCCACTG CTGCTTCGCC TCTCAGAGAA CTCCGGAGAT
    GACAACATGA GCGACGTGAC CTTTGACTCT CTCCCTTCCT CTCCGTCTTC AGCCACACCA
    CATAGCCAGA AGCTGGAGCA CCTCCATTGG CCTGTGTTCA GCGACCTTGA TAACATGGCA
    CCTCGAGACC AGGACCCTCT GGACAACCAC CGGGACTCTG AGAACGGTGG GGACAGTGGA
    TACCCCAGTG AGAAGCGGGG TGACCTGGAT GACCCAGAGC CCAGAGAACA TGGCCACTCA
    AACGGTAACC GGAGGTACGA GTCGGATGAA GACAGCCTGG GCAGCTCCGG ACGGGTCTGT
    GTGGAGAAGT GGAATCTCTT CAACTCCTCC CGCCTCCACC TGCCGAGACC TTCGGCCGTG
    GCCCTGGAAG TGCAGAGGCT CAACGCTCTG GACCTCGAGA AGAAAATCGG GAAGTCCATT
    TTGGGGAAGG TCCATCTGGC CATGGTGCGC TACCACGAGG CTGGCCGCTT CTGCGAGAAG
    GACGAGGAGT GGGACCGGGA GTCGGCGGTC TTCCACCTGG AGCACGCAGC TGACCTGGGC
    GAGCTGGAGG CCATCGTGGG CCTGGGACTC ATGTACTCGC AGCTGCCCCA CCACATCCTG
    GCGGATGTCT CTCTGAAGGA GACAGAAGAG AGCAAAACCA AAGGATTTGA TTACTTACTG
    AAGGCAGCTG AAGCTGGGGA CAGACAGTGC ATGATTCTGG TGGCACGAGC CTTTGACACT
    GGCCAGAACC TCAGCCCTGA CAGGTCCCAG AGCTGGCCAG AGGCTCTCCA CTGGTACAAC
    ACTGCCCTGG AGACAACTGA CTGTGATGAG GGTGGGGAGT ATGATGGGAT GCAAGACGAA
    CCCCGGTACA TGCTGCTGGC CAGGGAGGCG GAGATGCTGT TCACAGGCGG CTGCGGGCTG
    GAGAAGGACC CACAGAGATC AGGCGATTTG TACACCCAGG CAGCCGAGGC AGCGATGGAA
    GCCATGAAGG GCCGGCTGGC CAACCAGTAC TACGAGAAAG CAGAGGAGGC CTGGGCCCAG
    ATGGAGGAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa32973
    Clone ID Related Accession (Same CDS sequence) NM_001192542.1 , XM_005224739.3 , XM_005224739.4
    Accession Version XM_005224739.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2172bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product eukaryotic elongation factor 2 kinase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104572.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005224739.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCGAGG AAGACCTCAT CTTCCGCCTG GAAGGCGTGG ATGGCCGCCC AGCCTCCCGG 
    GCTGGTGATT CAGACACAGA CAGCGACGAT GATGAGGAGG GTTACTTCAT CTGCTCCATC
    ACCGATGACC CCGGGGCACA CCAGAATGTC AACTTCAAGG TTAATAACTG CCACAGCAAC
    CTAGCAAAAA GCGAGCGCTA CGGCTCCAGC GGGTCCCCGG CTGGCTCCTT CCATTTCAAG
    GAAGCTTGGA AGCATGCAAT CGAGAAAGCC AAGCACATGC CTGACCCCTG GGCTGAGTTC
    CACCTGGAAG ACGTTGCCAC GGAATGTGCC ACACGCCACA GGTACAACGC TGTTACCGGC
    GAGTGGGTGG AGGATGAAGT CCTAATCAAA ATGGCATCCC AGCCCTTCGG CCGGGGAGCA
    ATGAGGGAGT GCTTCAGAAC GAAGAAGCTC TCCAACTTCC TGCACACCCA GCAGTGGAAG
    GGGGCCTCCA ACTACGTGGC AAAGCGCTAC ATCGAGTCCG TGGACAGGGA CGTGTACTTC
    GAGGACGTGC GTCTACAGAT GGAGGCCAAG CTCTGGGGAG AGGAGTATAA TCGGCACAAG
    CCCCCCAAGC AGGTGGACAT CATGCAGATG TGCATCATAG AGCTGAAGGA GAGACCGGGC
    CGGCCGCTAT TCCACCTGGA ACACTACATC GAGGGCAAGT ACATCAAATA TAACTCCAAC
    TCAGGCTTCG TCCGTGATGA CAACGTGCGC CTGACGCCAC AGGCCTTCAG CCACTTCACG
    TTTGAGCGTT CCGGCCACCA GCTGATTGTG GTGGACATCC AGGGCGTGGG TGACCTCTAT
    ACCGACCCAC AGATCCACAC TGAGAGGGGC ACTGACTTTG GAGATGGAAA CCTAGGTGTC
    CGCGGGATGG CTCTCTTCTT CCACTCACAT GCCTGCAACC GGATTTGCAA GAGCATGGGC
    CTCACTCCCT TTGACCTGTC CCCGAGAGAG AGGGATGCAG TGAATCAGAA CACCAAGCTG
    CTGCAGTCAG CCAAGACCAT CTTAAGGGGG ACAGAGGAAA AGTGCGGGAG CCCCCGGGTG
    AGGACCCTCT CAGGGAGCCG GCCCCCACTG CTGCTTCGCC TCTCAGAGAA CTCCGGAGAT
    GACAACATGA GCGACGTGAC CTTTGACTCT CTCCCTTCCT CTCCGTCTTC AGCCACACCA
    CATAGCCAGA AGCTGGAGCA CCTCCATTGG CCTGTGTTCA GCGACCTTGA TAACATGGCA
    CCTCGAGACC AGGACCCTCT GGACAACCAC CGGGACTCTG AGAACGGTGG GGACAGTGGA
    TACCCCAGTG AGAAGCGGGG TGACCTGGAT GACCCAGAGC CCAGAGAACA TGGCCACTCA
    AACGGTAACC GGAGGTACGA GTCGGATGAA GACAGCCTGG GCAGCTCCGG ACGGGTCTGT
    GTGGAGAAGT GGAATCTCTT CAACTCCTCC CGCCTCCACC TGCCGAGACC TTCGGCCGTG
    GCCCTGGAAG TGCAGAGGCT CAACGCTCTG GACCTCGAGA AGAAAATCGG GAAGTCCATT
    TTGGGGAAGG TCCATCTGGC CATGGTGCGC TACCACGAGG CTGGCCGCTT CTGCGAGAAG
    GACGAGGAGT GGGACCGGGA GTCGGCGGTC TTCCACCTGG AGCACGCAGC TGACCTGGGC
    GAGCTGGAGG CCATCGTGGG CCTGGGACTC ATGTACTCGC AGCTGCCCCA CCACATCCTG
    GCGGATGTCT CTCTGAAGGA GACAGAAGAG AGCAAAACCA AAGGATTTGA TTACTTACTG
    AAGGCAGCTG AAGCTGGGGA CAGACAGTGC ATGATTCTGG TGGCACGAGC CTTTGACACT
    GGCCAGAACC TCAGCCCTGA CAGGTCCCAG AGCTGGCCAG AGGCTCTCCA CTGGTACAAC
    ACTGCCCTGG AGACAACTGA CTGTGATGAG GGTGGGGAGT ATGATGGGAT GCAAGACGAA
    CCCCGGTACA TGCTGCTGGC CAGGGAGGCG GAGATGCTGT TCACAGGCGG CTGCGGGCTG
    GAGAAGGACC CACAGAGATC AGGCGATTTG TACACCCAGG CAGCCGAGGC AGCGATGGAA
    GCCATGAAGG GCCGGCTGGC CAACCAGTAC TACGAGAAAG CAGAGGAGGC CTGGGCCCAG
    ATGGAGGAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005224796.1
    CDS178..2349
    Translation

    Target ORF information:

    RefSeq Version XM_005224739.3
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic elongation factor 2 kinase (EEF2K), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005224739.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCGAGG AAGACCTCAT CTTCCGCCTG GAAGGCGTGG ATGGCCGCCC AGCCTCCCGG 
    GCTGGTGATT CAGACACAGA CAGCGACGAT GATGAGGAGG GTTACTTCAT CTGCTCCATC
    ACCGATGACC CCGGGGCACA CCAGAATGTC AACTTCAAGG TTAATAACTG CCACAGCAAC
    CTAGCAAAAA GCGAGCGCTA CGGCTCCAGC GGGTCCCCGG CTGGCTCCTT CCATTTCAAG
    GAAGCTTGGA AGCATGCAAT CGAGAAAGCC AAGCACATGC CTGACCCCTG GGCTGAGTTC
    CACCTGGAAG ACGTTGCCAC GGAATGTGCC ACACGCCACA GGTACAACGC TGTTACCGGC
    GAGTGGGTGG AGGATGAAGT CCTAATCAAA ATGGCATCCC AGCCCTTCGG CCGGGGAGCA
    ATGAGGGAGT GCTTCAGAAC GAAGAAGCTC TCCAACTTCC TGCACACCCA GCAGTGGAAG
    GGGGCCTCCA ACTACGTGGC AAAGCGCTAC ATCGAGTCCG TGGACAGGGA CGTGTACTTC
    GAGGACGTGC GTCTACAGAT GGAGGCCAAG CTCTGGGGAG AGGAGTATAA TCGGCACAAG
    CCCCCCAAGC AGGTGGACAT CATGCAGATG TGCATCATAG AGCTGAAGGA GAGACCGGGC
    CGGCCGCTAT TCCACCTGGA ACACTACATC GAGGGCAAGT ACATCAAATA TAACTCCAAC
    TCAGGCTTCG TCCGTGATGA CAACGTGCGC CTGACGCCAC AGGCCTTCAG CCACTTCACG
    TTTGAGCGTT CCGGCCACCA GCTGATTGTG GTGGACATCC AGGGCGTGGG TGACCTCTAT
    ACCGACCCAC AGATCCACAC TGAGAGGGGC ACTGACTTTG GAGATGGAAA CCTAGGTGTC
    CGCGGGATGG CTCTCTTCTT CCACTCACAT GCCTGCAACC GGATTTGCAA GAGCATGGGC
    CTCACTCCCT TTGACCTGTC CCCGAGAGAG AGGGATGCAG TGAATCAGAA CACCAAGCTG
    CTGCAGTCAG CCAAGACCAT CTTAAGGGGG ACAGAGGAAA AGTGCGGGAG CCCCCGGGTG
    AGGACCCTCT CAGGGAGCCG GCCCCCACTG CTGCTTCGCC TCTCAGAGAA CTCCGGAGAT
    GACAACATGA GCGACGTGAC CTTTGACTCT CTCCCTTCCT CTCCGTCTTC AGCCACACCA
    CATAGCCAGA AGCTGGAGCA CCTCCATTGG CCTGTGTTCA GCGACCTTGA TAACATGGCA
    CCTCGAGACC AGGACCCTCT GGACAACCAC CGGGACTCTG AGAACGGTGG GGACAGTGGA
    TACCCCAGTG AGAAGCGGGG TGACCTGGAT GACCCAGAGC CCAGAGAACA TGGCCACTCA
    AACGGTAACC GGAGGTACGA GTCGGATGAA GACAGCCTGG GCAGCTCCGG ACGGGTCTGT
    GTGGAGAAGT GGAATCTCTT CAACTCCTCC CGCCTCCACC TGCCGAGACC TTCGGCCGTG
    GCCCTGGAAG TGCAGAGGCT CAACGCTCTG GACCTCGAGA AGAAAATCGG GAAGTCCATT
    TTGGGGAAGG TCCATCTGGC CATGGTGCGC TACCACGAGG CTGGCCGCTT CTGCGAGAAG
    GACGAGGAGT GGGACCGGGA GTCGGCGGTC TTCCACCTGG AGCACGCAGC TGACCTGGGC
    GAGCTGGAGG CCATCGTGGG CCTGGGACTC ATGTACTCGC AGCTGCCCCA CCACATCCTG
    GCGGATGTCT CTCTGAAGGA GACAGAAGAG AGCAAAACCA AAGGATTTGA TTACTTACTG
    AAGGCAGCTG AAGCTGGGGA CAGACAGTGC ATGATTCTGG TGGCACGAGC CTTTGACACT
    GGCCAGAACC TCAGCCCTGA CAGGTCCCAG AGCTGGCCAG AGGCTCTCCA CTGGTACAAC
    ACTGCCCTGG AGACAACTGA CTGTGATGAG GGTGGGGAGT ATGATGGGAT GCAAGACGAA
    CCCCGGTACA TGCTGCTGGC CAGGGAGGCG GAGATGCTGT TCACAGGCGG CTGCGGGCTG
    GAGAAGGACC CACAGAGATC AGGCGATTTG TACACCCAGG CAGCCGAGGC AGCGATGGAA
    GCCATGAAGG GCCGGCTGGC CAACCAGTAC TACGAGAAAG CAGAGGAGGC CTGGGCCCAG
    ATGGAGGAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa32973
    Clone ID Related Accession (Same CDS sequence) NM_001192542.1 , XM_005224739.3 , XM_005224739.4
    Accession Version XM_005224739.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2172bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product eukaryotic elongation factor 2 kinase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005224739.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCGAGG AAGACCTCAT CTTCCGCCTG GAAGGCGTGG ATGGCCGCCC AGCCTCCCGG 
    GCTGGTGATT CAGACACAGA CAGCGACGAT GATGAGGAGG GTTACTTCAT CTGCTCCATC
    ACCGATGACC CCGGGGCACA CCAGAATGTC AACTTCAAGG TTAATAACTG CCACAGCAAC
    CTAGCAAAAA GCGAGCGCTA CGGCTCCAGC GGGTCCCCGG CTGGCTCCTT CCATTTCAAG
    GAAGCTTGGA AGCATGCAAT CGAGAAAGCC AAGCACATGC CTGACCCCTG GGCTGAGTTC
    CACCTGGAAG ACGTTGCCAC GGAATGTGCC ACACGCCACA GGTACAACGC TGTTACCGGC
    GAGTGGGTGG AGGATGAAGT CCTAATCAAA ATGGCATCCC AGCCCTTCGG CCGGGGAGCA
    ATGAGGGAGT GCTTCAGAAC GAAGAAGCTC TCCAACTTCC TGCACACCCA GCAGTGGAAG
    GGGGCCTCCA ACTACGTGGC AAAGCGCTAC ATCGAGTCCG TGGACAGGGA CGTGTACTTC
    GAGGACGTGC GTCTACAGAT GGAGGCCAAG CTCTGGGGAG AGGAGTATAA TCGGCACAAG
    CCCCCCAAGC AGGTGGACAT CATGCAGATG TGCATCATAG AGCTGAAGGA GAGACCGGGC
    CGGCCGCTAT TCCACCTGGA ACACTACATC GAGGGCAAGT ACATCAAATA TAACTCCAAC
    TCAGGCTTCG TCCGTGATGA CAACGTGCGC CTGACGCCAC AGGCCTTCAG CCACTTCACG
    TTTGAGCGTT CCGGCCACCA GCTGATTGTG GTGGACATCC AGGGCGTGGG TGACCTCTAT
    ACCGACCCAC AGATCCACAC TGAGAGGGGC ACTGACTTTG GAGATGGAAA CCTAGGTGTC
    CGCGGGATGG CTCTCTTCTT CCACTCACAT GCCTGCAACC GGATTTGCAA GAGCATGGGC
    CTCACTCCCT TTGACCTGTC CCCGAGAGAG AGGGATGCAG TGAATCAGAA CACCAAGCTG
    CTGCAGTCAG CCAAGACCAT CTTAAGGGGG ACAGAGGAAA AGTGCGGGAG CCCCCGGGTG
    AGGACCCTCT CAGGGAGCCG GCCCCCACTG CTGCTTCGCC TCTCAGAGAA CTCCGGAGAT
    GACAACATGA GCGACGTGAC CTTTGACTCT CTCCCTTCCT CTCCGTCTTC AGCCACACCA
    CATAGCCAGA AGCTGGAGCA CCTCCATTGG CCTGTGTTCA GCGACCTTGA TAACATGGCA
    CCTCGAGACC AGGACCCTCT GGACAACCAC CGGGACTCTG AGAACGGTGG GGACAGTGGA
    TACCCCAGTG AGAAGCGGGG TGACCTGGAT GACCCAGAGC CCAGAGAACA TGGCCACTCA
    AACGGTAACC GGAGGTACGA GTCGGATGAA GACAGCCTGG GCAGCTCCGG ACGGGTCTGT
    GTGGAGAAGT GGAATCTCTT CAACTCCTCC CGCCTCCACC TGCCGAGACC TTCGGCCGTG
    GCCCTGGAAG TGCAGAGGCT CAACGCTCTG GACCTCGAGA AGAAAATCGG GAAGTCCATT
    TTGGGGAAGG TCCATCTGGC CATGGTGCGC TACCACGAGG CTGGCCGCTT CTGCGAGAAG
    GACGAGGAGT GGGACCGGGA GTCGGCGGTC TTCCACCTGG AGCACGCAGC TGACCTGGGC
    GAGCTGGAGG CCATCGTGGG CCTGGGACTC ATGTACTCGC AGCTGCCCCA CCACATCCTG
    GCGGATGTCT CTCTGAAGGA GACAGAAGAG AGCAAAACCA AAGGATTTGA TTACTTACTG
    AAGGCAGCTG AAGCTGGGGA CAGACAGTGC ATGATTCTGG TGGCACGAGC CTTTGACACT
    GGCCAGAACC TCAGCCCTGA CAGGTCCCAG AGCTGGCCAG AGGCTCTCCA CTGGTACAAC
    ACTGCCCTGG AGACAACTGA CTGTGATGAG GGTGGGGAGT ATGATGGGAT GCAAGACGAA
    CCCCGGTACA TGCTGCTGGC CAGGGAGGCG GAGATGCTGT TCACAGGCGG CTGCGGGCTG
    GAGAAGGACC CACAGAGATC AGGCGATTTG TACACCCAGG CAGCCGAGGC AGCGATGGAA
    GCCATGAAGG GCCGGCTGGC CAACCAGTAC TACGAGAAAG CAGAGGAGGC CTGGGCCCAG
    ATGGAGGAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005224796.1
    CDS481..2652
    Translation

    Target ORF information:

    RefSeq Version XM_005224739.4
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic elongation factor 2 kinase (EEF2K), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005224739.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCGAGG AAGACCTCAT CTTCCGCCTG GAAGGCGTGG ATGGCCGCCC AGCCTCCCGG 
    GCTGGTGATT CAGACACAGA CAGCGACGAT GATGAGGAGG GTTACTTCAT CTGCTCCATC
    ACCGATGACC CCGGGGCACA CCAGAATGTC AACTTCAAGG TTAATAACTG CCACAGCAAC
    CTAGCAAAAA GCGAGCGCTA CGGCTCCAGC GGGTCCCCGG CTGGCTCCTT CCATTTCAAG
    GAAGCTTGGA AGCATGCAAT CGAGAAAGCC AAGCACATGC CTGACCCCTG GGCTGAGTTC
    CACCTGGAAG ACGTTGCCAC GGAATGTGCC ACACGCCACA GGTACAACGC TGTTACCGGC
    GAGTGGGTGG AGGATGAAGT CCTAATCAAA ATGGCATCCC AGCCCTTCGG CCGGGGAGCA
    ATGAGGGAGT GCTTCAGAAC GAAGAAGCTC TCCAACTTCC TGCACACCCA GCAGTGGAAG
    GGGGCCTCCA ACTACGTGGC AAAGCGCTAC ATCGAGTCCG TGGACAGGGA CGTGTACTTC
    GAGGACGTGC GTCTACAGAT GGAGGCCAAG CTCTGGGGAG AGGAGTATAA TCGGCACAAG
    CCCCCCAAGC AGGTGGACAT CATGCAGATG TGCATCATAG AGCTGAAGGA GAGACCGGGC
    CGGCCGCTAT TCCACCTGGA ACACTACATC GAGGGCAAGT ACATCAAATA TAACTCCAAC
    TCAGGCTTCG TCCGTGATGA CAACGTGCGC CTGACGCCAC AGGCCTTCAG CCACTTCACG
    TTTGAGCGTT CCGGCCACCA GCTGATTGTG GTGGACATCC AGGGCGTGGG TGACCTCTAT
    ACCGACCCAC AGATCCACAC TGAGAGGGGC ACTGACTTTG GAGATGGAAA CCTAGGTGTC
    CGCGGGATGG CTCTCTTCTT CCACTCACAT GCCTGCAACC GGATTTGCAA GAGCATGGGC
    CTCACTCCCT TTGACCTGTC CCCGAGAGAG AGGGATGCAG TGAATCAGAA CACCAAGCTG
    CTGCAGTCAG CCAAGACCAT CTTAAGGGGG ACAGAGGAAA AGTGCGGGAG CCCCCGGGTG
    AGGACCCTCT CAGGGAGCCG GCCCCCACTG CTGCTTCGCC TCTCAGAGAA CTCCGGAGAT
    GACAACATGA GCGACGTGAC CTTTGACTCT CTCCCTTCCT CTCCGTCTTC AGCCACACCA
    CATAGCCAGA AGCTGGAGCA CCTCCATTGG CCTGTGTTCA GCGACCTTGA TAACATGGCA
    CCTCGAGACC AGGACCCTCT GGACAACCAC CGGGACTCTG AGAACGGTGG GGACAGTGGA
    TACCCCAGTG AGAAGCGGGG TGACCTGGAT GACCCAGAGC CCAGAGAACA TGGCCACTCA
    AACGGTAACC GGAGGTACGA GTCGGATGAA GACAGCCTGG GCAGCTCCGG ACGGGTCTGT
    GTGGAGAAGT GGAATCTCTT CAACTCCTCC CGCCTCCACC TGCCGAGACC TTCGGCCGTG
    GCCCTGGAAG TGCAGAGGCT CAACGCTCTG GACCTCGAGA AGAAAATCGG GAAGTCCATT
    TTGGGGAAGG TCCATCTGGC CATGGTGCGC TACCACGAGG CTGGCCGCTT CTGCGAGAAG
    GACGAGGAGT GGGACCGGGA GTCGGCGGTC TTCCACCTGG AGCACGCAGC TGACCTGGGC
    GAGCTGGAGG CCATCGTGGG CCTGGGACTC ATGTACTCGC AGCTGCCCCA CCACATCCTG
    GCGGATGTCT CTCTGAAGGA GACAGAAGAG AGCAAAACCA AAGGATTTGA TTACTTACTG
    AAGGCAGCTG AAGCTGGGGA CAGACAGTGC ATGATTCTGG TGGCACGAGC CTTTGACACT
    GGCCAGAACC TCAGCCCTGA CAGGTCCCAG AGCTGGCCAG AGGCTCTCCA CTGGTACAAC
    ACTGCCCTGG AGACAACTGA CTGTGATGAG GGTGGGGAGT ATGATGGGAT GCAAGACGAA
    CCCCGGTACA TGCTGCTGGC CAGGGAGGCG GAGATGCTGT TCACAGGCGG CTGCGGGCTG
    GAGAAGGACC CACAGAGATC AGGCGATTTG TACACCCAGG CAGCCGAGGC AGCGATGGAA
    GCCATGAAGG GCCGGCTGGC CAACCAGTAC TACGAGAAAG CAGAGGAGGC CTGGGCCCAG
    ATGGAGGAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa32974
    Clone ID Related Accession (Same CDS sequence) XM_005224740.3 , XM_005224740.4
    Accession Version XM_005224740.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2169bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product eukaryotic elongation factor 2 kinase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104572.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005224740.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCGAGG AAGACCTCAT CTTCCGCCTG GAAGGCGTGG ATGGCCGCCC AGCCTCCCGG 
    GCTGGTGATT CAGACACAGA CAGCGACGAT GATGAGGAGG GTTACTTCAT CTGCTCCATC
    ACCGATGACC CCGGGGCACA CCAGAATGTC AACTTCAAGG TTAATAACTG CCACAGCAAC
    CTAGCAAAAA GCGAGCGCTA CGGCTCCAGC GGGTCCCCGG CTGGCTCCTT CCATTTCAAG
    GAAGCTTGGA AGCATGCAAT CGAGAAAGCC AAGCACATGC CTGACCCCTG GGCTGAGTTC
    CACCTGGAAG ACGTTGCCAC GGAATGTGCC ACACGCCACA GGTACAACGC TGTTACCGGC
    GAGTGGGTGG AGGATGAAGT CCTAATCAAA ATGGCATCCC AGCCCTTCGG CCGGGGAGCA
    ATGAGGGAGT GCTTCAGAAC GAAGAAGCTC TCCAACTTCC TGCACACCCA GCAGTGGAAG
    GGGGCCTCCA ACTACGTGGC AAAGCGCTAC ATCGAGTCCG TGGACAGGGA CGTGTACTTC
    GAGGACGTGC GTCTACAGAT GGAGGCCAAG CTCTGGGGAG AGGAGTATAA TCGGCACAAG
    CCCCCCAAGC AGGTGGACAT CATGCAGATG TGCATCATAG AGCTGAAGGA GAGACCGGGC
    CGGCCGCTAT TCCACCTGGA ACACTACATC GAGGGCAAGT ACATCAAATA TAACTCCAAC
    TCAGGCTTCG TCCGTGATGA CAACGTGCGC CTGACGCCAC AGGCCTTCAG CCACTTCACG
    TTTGAGCGTT CCGGCCACCA GCTGATTGTG GTGGACATCC AGGGCGTGGG TGACCTCTAT
    ACCGACCCAC AGATCCACAC TGAGAGGGGC ACTGACTTTG GAGATGGAAA CCTAGGTGTC
    CGCGGGATGG CTCTCTTCTT CCACTCACAT GCCTGCAACC GGATTTGCAA GAGCATGGGC
    CTCACTCCCT TTGACCTGTC CCCGAGAGAG AGGGATGCAG TGAATCAGAA CACCAAGCTG
    CTGTCAGCCA AGACCATCTT AAGGGGGACA GAGGAAAAGT GCGGGAGCCC CCGGGTGAGG
    ACCCTCTCAG GGAGCCGGCC CCCACTGCTG CTTCGCCTCT CAGAGAACTC CGGAGATGAC
    AACATGAGCG ACGTGACCTT TGACTCTCTC CCTTCCTCTC CGTCTTCAGC CACACCACAT
    AGCCAGAAGC TGGAGCACCT CCATTGGCCT GTGTTCAGCG ACCTTGATAA CATGGCACCT
    CGAGACCAGG ACCCTCTGGA CAACCACCGG GACTCTGAGA ACGGTGGGGA CAGTGGATAC
    CCCAGTGAGA AGCGGGGTGA CCTGGATGAC CCAGAGCCCA GAGAACATGG CCACTCAAAC
    GGTAACCGGA GGTACGAGTC GGATGAAGAC AGCCTGGGCA GCTCCGGACG GGTCTGTGTG
    GAGAAGTGGA ATCTCTTCAA CTCCTCCCGC CTCCACCTGC CGAGACCTTC GGCCGTGGCC
    CTGGAAGTGC AGAGGCTCAA CGCTCTGGAC CTCGAGAAGA AAATCGGGAA GTCCATTTTG
    GGGAAGGTCC ATCTGGCCAT GGTGCGCTAC CACGAGGCTG GCCGCTTCTG CGAGAAGGAC
    GAGGAGTGGG ACCGGGAGTC GGCGGTCTTC CACCTGGAGC ACGCAGCTGA CCTGGGCGAG
    CTGGAGGCCA TCGTGGGCCT GGGACTCATG TACTCGCAGC TGCCCCACCA CATCCTGGCG
    GATGTCTCTC TGAAGGAGAC AGAAGAGAGC AAAACCAAAG GATTTGATTA CTTACTGAAG
    GCAGCTGAAG CTGGGGACAG ACAGTGCATG ATTCTGGTGG CACGAGCCTT TGACACTGGC
    CAGAACCTCA GCCCTGACAG GTCCCAGAGC TGGCCAGAGG CTCTCCACTG GTACAACACT
    GCCCTGGAGA CAACTGACTG TGATGAGGGT GGGGAGTATG ATGGGATGCA AGACGAACCC
    CGGTACATGC TGCTGGCCAG GGAGGCGGAG ATGCTGTTCA CAGGCGGCTG CGGGCTGGAG
    AAGGACCCAC AGAGATCAGG CGATTTGTAC ACCCAGGCAG CCGAGGCAGC GATGGAAGCC
    ATGAAGGGCC GGCTGGCCAA CCAGTACTAC GAGAAAGCAG AGGAGGCCTG GGCCCAGATG
    GAGGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005224797.1
    CDS180..2348
    Translation

    Target ORF information:

    RefSeq Version XM_005224740.3
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic elongation factor 2 kinase (EEF2K), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005224740.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCGAGG AAGACCTCAT CTTCCGCCTG GAAGGCGTGG ATGGCCGCCC AGCCTCCCGG 
    GCTGGTGATT CAGACACAGA CAGCGACGAT GATGAGGAGG GTTACTTCAT CTGCTCCATC
    ACCGATGACC CCGGGGCACA CCAGAATGTC AACTTCAAGG TTAATAACTG CCACAGCAAC
    CTAGCAAAAA GCGAGCGCTA CGGCTCCAGC GGGTCCCCGG CTGGCTCCTT CCATTTCAAG
    GAAGCTTGGA AGCATGCAAT CGAGAAAGCC AAGCACATGC CTGACCCCTG GGCTGAGTTC
    CACCTGGAAG ACGTTGCCAC GGAATGTGCC ACACGCCACA GGTACAACGC TGTTACCGGC
    GAGTGGGTGG AGGATGAAGT CCTAATCAAA ATGGCATCCC AGCCCTTCGG CCGGGGAGCA
    ATGAGGGAGT GCTTCAGAAC GAAGAAGCTC TCCAACTTCC TGCACACCCA GCAGTGGAAG
    GGGGCCTCCA ACTACGTGGC AAAGCGCTAC ATCGAGTCCG TGGACAGGGA CGTGTACTTC
    GAGGACGTGC GTCTACAGAT GGAGGCCAAG CTCTGGGGAG AGGAGTATAA TCGGCACAAG
    CCCCCCAAGC AGGTGGACAT CATGCAGATG TGCATCATAG AGCTGAAGGA GAGACCGGGC
    CGGCCGCTAT TCCACCTGGA ACACTACATC GAGGGCAAGT ACATCAAATA TAACTCCAAC
    TCAGGCTTCG TCCGTGATGA CAACGTGCGC CTGACGCCAC AGGCCTTCAG CCACTTCACG
    TTTGAGCGTT CCGGCCACCA GCTGATTGTG GTGGACATCC AGGGCGTGGG TGACCTCTAT
    ACCGACCCAC AGATCCACAC TGAGAGGGGC ACTGACTTTG GAGATGGAAA CCTAGGTGTC
    CGCGGGATGG CTCTCTTCTT CCACTCACAT GCCTGCAACC GGATTTGCAA GAGCATGGGC
    CTCACTCCCT TTGACCTGTC CCCGAGAGAG AGGGATGCAG TGAATCAGAA CACCAAGCTG
    CTGTCAGCCA AGACCATCTT AAGGGGGACA GAGGAAAAGT GCGGGAGCCC CCGGGTGAGG
    ACCCTCTCAG GGAGCCGGCC CCCACTGCTG CTTCGCCTCT CAGAGAACTC CGGAGATGAC
    AACATGAGCG ACGTGACCTT TGACTCTCTC CCTTCCTCTC CGTCTTCAGC CACACCACAT
    AGCCAGAAGC TGGAGCACCT CCATTGGCCT GTGTTCAGCG ACCTTGATAA CATGGCACCT
    CGAGACCAGG ACCCTCTGGA CAACCACCGG GACTCTGAGA ACGGTGGGGA CAGTGGATAC
    CCCAGTGAGA AGCGGGGTGA CCTGGATGAC CCAGAGCCCA GAGAACATGG CCACTCAAAC
    GGTAACCGGA GGTACGAGTC GGATGAAGAC AGCCTGGGCA GCTCCGGACG GGTCTGTGTG
    GAGAAGTGGA ATCTCTTCAA CTCCTCCCGC CTCCACCTGC CGAGACCTTC GGCCGTGGCC
    CTGGAAGTGC AGAGGCTCAA CGCTCTGGAC CTCGAGAAGA AAATCGGGAA GTCCATTTTG
    GGGAAGGTCC ATCTGGCCAT GGTGCGCTAC CACGAGGCTG GCCGCTTCTG CGAGAAGGAC
    GAGGAGTGGG ACCGGGAGTC GGCGGTCTTC CACCTGGAGC ACGCAGCTGA CCTGGGCGAG
    CTGGAGGCCA TCGTGGGCCT GGGACTCATG TACTCGCAGC TGCCCCACCA CATCCTGGCG
    GATGTCTCTC TGAAGGAGAC AGAAGAGAGC AAAACCAAAG GATTTGATTA CTTACTGAAG
    GCAGCTGAAG CTGGGGACAG ACAGTGCATG ATTCTGGTGG CACGAGCCTT TGACACTGGC
    CAGAACCTCA GCCCTGACAG GTCCCAGAGC TGGCCAGAGG CTCTCCACTG GTACAACACT
    GCCCTGGAGA CAACTGACTG TGATGAGGGT GGGGAGTATG ATGGGATGCA AGACGAACCC
    CGGTACATGC TGCTGGCCAG GGAGGCGGAG ATGCTGTTCA CAGGCGGCTG CGGGCTGGAG
    AAGGACCCAC AGAGATCAGG CGATTTGTAC ACCCAGGCAG CCGAGGCAGC GATGGAAGCC
    ATGAAGGGCC GGCTGGCCAA CCAGTACTAC GAGAAAGCAG AGGAGGCCTG GGCCCAGATG
    GAGGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa32974
    Clone ID Related Accession (Same CDS sequence) XM_005224740.3 , XM_005224740.4
    Accession Version XM_005224740.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2169bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product eukaryotic elongation factor 2 kinase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005224740.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCGAGG AAGACCTCAT CTTCCGCCTG GAAGGCGTGG ATGGCCGCCC AGCCTCCCGG 
    GCTGGTGATT CAGACACAGA CAGCGACGAT GATGAGGAGG GTTACTTCAT CTGCTCCATC
    ACCGATGACC CCGGGGCACA CCAGAATGTC AACTTCAAGG TTAATAACTG CCACAGCAAC
    CTAGCAAAAA GCGAGCGCTA CGGCTCCAGC GGGTCCCCGG CTGGCTCCTT CCATTTCAAG
    GAAGCTTGGA AGCATGCAAT CGAGAAAGCC AAGCACATGC CTGACCCCTG GGCTGAGTTC
    CACCTGGAAG ACGTTGCCAC GGAATGTGCC ACACGCCACA GGTACAACGC TGTTACCGGC
    GAGTGGGTGG AGGATGAAGT CCTAATCAAA ATGGCATCCC AGCCCTTCGG CCGGGGAGCA
    ATGAGGGAGT GCTTCAGAAC GAAGAAGCTC TCCAACTTCC TGCACACCCA GCAGTGGAAG
    GGGGCCTCCA ACTACGTGGC AAAGCGCTAC ATCGAGTCCG TGGACAGGGA CGTGTACTTC
    GAGGACGTGC GTCTACAGAT GGAGGCCAAG CTCTGGGGAG AGGAGTATAA TCGGCACAAG
    CCCCCCAAGC AGGTGGACAT CATGCAGATG TGCATCATAG AGCTGAAGGA GAGACCGGGC
    CGGCCGCTAT TCCACCTGGA ACACTACATC GAGGGCAAGT ACATCAAATA TAACTCCAAC
    TCAGGCTTCG TCCGTGATGA CAACGTGCGC CTGACGCCAC AGGCCTTCAG CCACTTCACG
    TTTGAGCGTT CCGGCCACCA GCTGATTGTG GTGGACATCC AGGGCGTGGG TGACCTCTAT
    ACCGACCCAC AGATCCACAC TGAGAGGGGC ACTGACTTTG GAGATGGAAA CCTAGGTGTC
    CGCGGGATGG CTCTCTTCTT CCACTCACAT GCCTGCAACC GGATTTGCAA GAGCATGGGC
    CTCACTCCCT TTGACCTGTC CCCGAGAGAG AGGGATGCAG TGAATCAGAA CACCAAGCTG
    CTGTCAGCCA AGACCATCTT AAGGGGGACA GAGGAAAAGT GCGGGAGCCC CCGGGTGAGG
    ACCCTCTCAG GGAGCCGGCC CCCACTGCTG CTTCGCCTCT CAGAGAACTC CGGAGATGAC
    AACATGAGCG ACGTGACCTT TGACTCTCTC CCTTCCTCTC CGTCTTCAGC CACACCACAT
    AGCCAGAAGC TGGAGCACCT CCATTGGCCT GTGTTCAGCG ACCTTGATAA CATGGCACCT
    CGAGACCAGG ACCCTCTGGA CAACCACCGG GACTCTGAGA ACGGTGGGGA CAGTGGATAC
    CCCAGTGAGA AGCGGGGTGA CCTGGATGAC CCAGAGCCCA GAGAACATGG CCACTCAAAC
    GGTAACCGGA GGTACGAGTC GGATGAAGAC AGCCTGGGCA GCTCCGGACG GGTCTGTGTG
    GAGAAGTGGA ATCTCTTCAA CTCCTCCCGC CTCCACCTGC CGAGACCTTC GGCCGTGGCC
    CTGGAAGTGC AGAGGCTCAA CGCTCTGGAC CTCGAGAAGA AAATCGGGAA GTCCATTTTG
    GGGAAGGTCC ATCTGGCCAT GGTGCGCTAC CACGAGGCTG GCCGCTTCTG CGAGAAGGAC
    GAGGAGTGGG ACCGGGAGTC GGCGGTCTTC CACCTGGAGC ACGCAGCTGA CCTGGGCGAG
    CTGGAGGCCA TCGTGGGCCT GGGACTCATG TACTCGCAGC TGCCCCACCA CATCCTGGCG
    GATGTCTCTC TGAAGGAGAC AGAAGAGAGC AAAACCAAAG GATTTGATTA CTTACTGAAG
    GCAGCTGAAG CTGGGGACAG ACAGTGCATG ATTCTGGTGG CACGAGCCTT TGACACTGGC
    CAGAACCTCA GCCCTGACAG GTCCCAGAGC TGGCCAGAGG CTCTCCACTG GTACAACACT
    GCCCTGGAGA CAACTGACTG TGATGAGGGT GGGGAGTATG ATGGGATGCA AGACGAACCC
    CGGTACATGC TGCTGGCCAG GGAGGCGGAG ATGCTGTTCA CAGGCGGCTG CGGGCTGGAG
    AAGGACCCAC AGAGATCAGG CGATTTGTAC ACCCAGGCAG CCGAGGCAGC GATGGAAGCC
    ATGAAGGGCC GGCTGGCCAA CCAGTACTAC GAGAAAGCAG AGGAGGCCTG GGCCCAGATG
    GAGGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005224797.1
    CDS400..2568
    Translation

    Target ORF information:

    RefSeq Version XM_005224740.4
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic elongation factor 2 kinase (EEF2K), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005224740.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCGAGG AAGACCTCAT CTTCCGCCTG GAAGGCGTGG ATGGCCGCCC AGCCTCCCGG 
    GCTGGTGATT CAGACACAGA CAGCGACGAT GATGAGGAGG GTTACTTCAT CTGCTCCATC
    ACCGATGACC CCGGGGCACA CCAGAATGTC AACTTCAAGG TTAATAACTG CCACAGCAAC
    CTAGCAAAAA GCGAGCGCTA CGGCTCCAGC GGGTCCCCGG CTGGCTCCTT CCATTTCAAG
    GAAGCTTGGA AGCATGCAAT CGAGAAAGCC AAGCACATGC CTGACCCCTG GGCTGAGTTC
    CACCTGGAAG ACGTTGCCAC GGAATGTGCC ACACGCCACA GGTACAACGC TGTTACCGGC
    GAGTGGGTGG AGGATGAAGT CCTAATCAAA ATGGCATCCC AGCCCTTCGG CCGGGGAGCA
    ATGAGGGAGT GCTTCAGAAC GAAGAAGCTC TCCAACTTCC TGCACACCCA GCAGTGGAAG
    GGGGCCTCCA ACTACGTGGC AAAGCGCTAC ATCGAGTCCG TGGACAGGGA CGTGTACTTC
    GAGGACGTGC GTCTACAGAT GGAGGCCAAG CTCTGGGGAG AGGAGTATAA TCGGCACAAG
    CCCCCCAAGC AGGTGGACAT CATGCAGATG TGCATCATAG AGCTGAAGGA GAGACCGGGC
    CGGCCGCTAT TCCACCTGGA ACACTACATC GAGGGCAAGT ACATCAAATA TAACTCCAAC
    TCAGGCTTCG TCCGTGATGA CAACGTGCGC CTGACGCCAC AGGCCTTCAG CCACTTCACG
    TTTGAGCGTT CCGGCCACCA GCTGATTGTG GTGGACATCC AGGGCGTGGG TGACCTCTAT
    ACCGACCCAC AGATCCACAC TGAGAGGGGC ACTGACTTTG GAGATGGAAA CCTAGGTGTC
    CGCGGGATGG CTCTCTTCTT CCACTCACAT GCCTGCAACC GGATTTGCAA GAGCATGGGC
    CTCACTCCCT TTGACCTGTC CCCGAGAGAG AGGGATGCAG TGAATCAGAA CACCAAGCTG
    CTGTCAGCCA AGACCATCTT AAGGGGGACA GAGGAAAAGT GCGGGAGCCC CCGGGTGAGG
    ACCCTCTCAG GGAGCCGGCC CCCACTGCTG CTTCGCCTCT CAGAGAACTC CGGAGATGAC
    AACATGAGCG ACGTGACCTT TGACTCTCTC CCTTCCTCTC CGTCTTCAGC CACACCACAT
    AGCCAGAAGC TGGAGCACCT CCATTGGCCT GTGTTCAGCG ACCTTGATAA CATGGCACCT
    CGAGACCAGG ACCCTCTGGA CAACCACCGG GACTCTGAGA ACGGTGGGGA CAGTGGATAC
    CCCAGTGAGA AGCGGGGTGA CCTGGATGAC CCAGAGCCCA GAGAACATGG CCACTCAAAC
    GGTAACCGGA GGTACGAGTC GGATGAAGAC AGCCTGGGCA GCTCCGGACG GGTCTGTGTG
    GAGAAGTGGA ATCTCTTCAA CTCCTCCCGC CTCCACCTGC CGAGACCTTC GGCCGTGGCC
    CTGGAAGTGC AGAGGCTCAA CGCTCTGGAC CTCGAGAAGA AAATCGGGAA GTCCATTTTG
    GGGAAGGTCC ATCTGGCCAT GGTGCGCTAC CACGAGGCTG GCCGCTTCTG CGAGAAGGAC
    GAGGAGTGGG ACCGGGAGTC GGCGGTCTTC CACCTGGAGC ACGCAGCTGA CCTGGGCGAG
    CTGGAGGCCA TCGTGGGCCT GGGACTCATG TACTCGCAGC TGCCCCACCA CATCCTGGCG
    GATGTCTCTC TGAAGGAGAC AGAAGAGAGC AAAACCAAAG GATTTGATTA CTTACTGAAG
    GCAGCTGAAG CTGGGGACAG ACAGTGCATG ATTCTGGTGG CACGAGCCTT TGACACTGGC
    CAGAACCTCA GCCCTGACAG GTCCCAGAGC TGGCCAGAGG CTCTCCACTG GTACAACACT
    GCCCTGGAGA CAACTGACTG TGATGAGGGT GGGGAGTATG ATGGGATGCA AGACGAACCC
    CGGTACATGC TGCTGGCCAG GGAGGCGGAG ATGCTGTTCA CAGGCGGCTG CGGGCTGGAG
    AAGGACCCAC AGAGATCAGG CGATTTGTAC ACCCAGGCAG CCGAGGCAGC GATGGAAGCC
    ATGAAGGGCC GGCTGGCCAA CCAGTACTAC GAGAAAGCAG AGGAGGCCTG GGCCCAGATG
    GAGGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.