PFAS cDNA ORF clone, Bos taurus(cattle)

The following PFAS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PFAS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa226375 XM_024980168.1
Latest version!
Bos taurus phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa226375 XM_024980167.1
Latest version!
Bos taurus phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa226376 XM_015458726.2
Latest version!
Bos taurus phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa226377 XM_005220359.4
Latest version!
Bos taurus phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa93869 XM_015458726.1
Latest version!
Bos taurus phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OBa93870 XM_005220359.3
Latest version!
Bos taurus phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OBa29748 NM_001256564.1
Latest version!
Bos taurus phosphoribosylformylglycinamidine synthase (PFAS), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa29748 XM_010816030.2
Latest version!
Bos taurus phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa226375
    Clone ID Related Accession (Same CDS sequence) XM_024980167.1 , XM_024980168.1
    Accession Version XM_024980167.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4017bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product phosphoribosylformylglycinamidine synthase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTCCTCCG TCCTTCACTT CTATGTCCGC CCCTCTGGCC ACGAGAGGGC AGCCTCTGAA 
    TACATTCAAA GGAAGCTGCA AAGGGAACTG CCAGAGCTGC AGGGTGTCAA GACCGAGCAG
    TGCTACAATG TGAACTGGAC AGCGGAGTCC TTCCCGAGCA ACAAGGAGAT GAAGAAGCTG
    ACATGGCTAT TTGGCTGCCC CTTGTTACTG GACGATGTTG CTCAGGAATC CTGGCTTCGT
    CCTGGCCCCA CAGACCTGCT GCTGGAGGTC GGACCCAGGC TGAACTTTTC CACCCCGACA
    TCCAGCAACA TTGTGTCAGT GTGCCAGGCT GCAGGGCTGG GGGCTGTGGA CCGCGTGGAG
    CCAACCCGGC GCTACCTGCT CTCGTTTGCC CACCCACCTT CAGCTGACCT GGAGGCCATT
    GCCCTGGCTA CCCTGCATGA CCGGATGACA GAGCAGCGCT TCCCCCAGCC CATCCAGAGC
    TTCTCTTCAG GGTGCATCCC AGCACCCCTG AGCGGCCCAA TTGACGTGCT GGCTGAGGGC
    CGGTCTGCCC TGGAGAAAGC CAACCAGGAG CTAGGTCTGG CCCTAGACTC CTGGGACCTG
    GATTTCTACA CCAAGCGCTT CCAGGAGCTG CAGCGCAACC CCAGCACTGT GGAAGTCTTC
    GACTTGGCTC AGTCCAATAG CGAACACAGC CGACACTGGT TCTTCAAGGG TCGACTCCAT
    GTGGATGGAC AGGAGCTGCC ACACTCGCTG TTTGAGTCCA TCATGAGCAC CCAGGCGTCC
    TCCAACCCTA ACAACGTCCT CAAGTTCTGT GACAACAGCA GTGCCATCCA GGGGAAAGAA
    GTCCGATTCT TGCGGCCTAA GGACCCCACA CGGCCAAGCT CCTTCCAGCA ATGTCAGGGG
    CTGAGACATG TTGTCTTCAC AGCAGAGACA CACAACTTCC CTACAGGAGT AGCCCCCTTC
    AGTGGTGCAA CCACTGGCAC AGGGGGCCGG ATCCGAGATG TCCAGTGCAC AGGCCGAGGG
    GCCCACGTGG TGGCTGGCAC TGCTGGCTAT TGCTTTGGAA ACCTGCACAT CCCAGGTTAC
    AGTATGCCCT GGGAGGATCC AAGCTTCCAG TATCCAGGAA ACTTTGCCCG ACCCCTGGAG
    ATTGCCATTG AGGCCAGTAA TGGGGCTTCT GACTACGGCA ACAAGTTTGG GGAACCAGTG
    CTTGCTGGCT TTGCCCGCTC CTTGGGCCTT CAGCTCCCGG ACGGCCAGCG GCGTGAGTGG
    ATCAAGCCCA TCATGTTTAG TGGAGGCATT GGGTCCATGG AAGCTGATCA TGTGAGCAAG
    GAGCCCCCAG AGACAGGCAT GGATGTCGTG AAGATTGGCG GTCCTGTCTA CAGGATTGGA
    GTTGGGGGCG GAGCTGCTTC GTCTGTGCAG GTGCAAGGAG ACAACGCCAG TGACCTGGAT
    TTTGGGGCTG TGCAGCGGGG AGACCCAGAA ATGGAGCAGA AGATGAACCG TGTGATCCGG
    GCTTGTGTGG AGGCCCCTGG GGGAAACCCC ATCTGCAGCC TCCATGACCA GGGTGCTGGT
    GGCAATGGCA ATGTCCTGAA GGAGCTGAGT GACCCGGCTG GAGCCATCAT TCACACCAGC
    TGCTTCCAGC TGGGGGACCC AACCCTGAAT GCCCTGGAAA TCTGGGGGGC CGAGTACCAG
    GAGTCGAACG CACTTCTGCT GAGGCCCTCT GACCGGGACT TCCTGAGTTG TGTCAGCGCC
    CGGGAACGCT GCCCAGTCTG CTTTGTGGGC ACCATCACTG GAGACAGGAG AATAGTGCTG
    GTGGATGATC GGGAGTGGCC TATGGGCACA GGTGGCCAGG TGGATGCGCC CCCCACTCCT
    CCCCCAACCC CTGTGGACCT GGACCTGGAC TGGGTGCTGG GCAAGATGCC CCAGAAGGAG
    TTCTTCCTCC AGAGGAGTCT CCCCTTGCTG CAGCCTCTGA CTCTGCCCCC AGGGCTGAGC
    GTGCGCCAGG CTCTGGCGCG GGTCCTCAGG CTGCCTGCCG TGGCCAGCAA GCGCTACCTC
    ACCAACAAGG TGGACCGTTC TGTGGGCGGC CTGGTGGCCC AGCAGCAGTG TGTGGGACCC
    CTGCAGACCC CTCTGGCAGA TGTGGCGGTT GTGGCACTGA GTCACCAGGA GCTTGTAGGG
    GCCGCCACAG CCCTGGGAGA GCAGCCAGTC AAGAGCCTGC TGGACCCGAA GGTTGCCGCC
    CGGCTGGCTG TGGCCGAAGC CCTCACCAAC CTGGTATTTG CTCTGGTCAC CGACCTCCAG
    GATGTGAAAT GCAGCGGGAA CTGGATGTGG GCAGCCAAGC TCCCAGGGGA GGGTGCAGCT
    CTGGCTGACG CCTGTGAGGC TATGGTGGCA GTGATGGCGG CCCTGGGTGT AGCGGTAGAC
    GGTGGCAAGG ACTCCCTCAG CATGGCTGCC CGGGTTGGCT CTGAAACCGT GCGGGCTCCT
    GGGTCACTGG TCATCTCAGC CTATGCTGTC TGTTCAGACA TTACAGCCAC TGTGACCCCA
    GACCTCAAGC GTCCTGGGGG GAGAGGCCAC CTGCTCTACG TGCCTCTGAG TCCTGGGCAG
    CACCGGCTGG GGGGCACAGC TCTGGCTCAG TGCTTCTCCC AGCTTGGCGA GCAGCCTCCC
    GACCTGGATG TTCCTGAGAA CTTGGTGCGA GCCTTCAGCA TCACCCAGGG GCTGCTGAAA
    GACCGCCTCC TCTGCTCAGG CCATGATGTC AGTGATGGAG GTCTCATCAC TTGCCTGCTA
    GAGATGGCCT TTGCTGGGAA TTGTGGGATA GAGGTGGACA TCCCCGCACC TGGGGTCGAT
    GCCCTGCCTG TGCTGTTCGC CGAGGAGCCA GGCCTGGTGC TGGAGGTGCA GGAGCCGGAC
    CTCGCCCAAG TGCTGATGCG TTACCGGAAT GCTGGCCTCC ACTGCCTAGA GCTGGGTCTC
    ACAGGCGACA CGGGGCCCCA CGCCATGGTC CGCGTGTCGG TGAACAGGAC GGTGGTTCTG
    GAGGAACCTG TTGGGCAGCT ACGAGCCCTC TGGGAGGAAA CCAGTTTCCA GTTGGACCGG
    CTACAGGCAA AACCACACTG TGTGGCCCAG GAAGAGCAAG GGCTGAGGGA GCGGGCAGGG
    CCCACCTTCT GCCTGCCGCC TACCTTCCCC AGAGCTTCCG TGCCTTGTGA GCCTGGTGGT
    CCTGCCCCCC GAGTCGCCAT CATTCGGGAA GAGGGCAGTA ATGGAGACCG GGAGATGGCA
    GATGCCTTCC ACTTGGCTGG ATTTGAGGTG TGGGATGTAA CCATGCAGGA CCTGTGCTCT
    GGAGCCATCG GGCTGGACAC ATTCCGCGGT GTGGCCTTCG TAGGCGGCTT CAGCTACGCG
    GACGTCCTGG GCTCTGCCAA AGGCTGGGCA GCTGCTGTGA CCTTTAACCC GCAGGCCGGG
    GCTGAGATGA AGCGCTTCCG CCAGCGGCCA GACACCTTCA GCCTGGGCGT GTGTAACGGC
    TGTCAGCTGC TGGCCCTGCT GGGCTGGGTG GGAGGCAGTT CTAGTGAGGA GGTGGTGGAG
    ATGTGCCAGG AGTCCTGGCC GGCCCGGCCC GGTCTCCTGC TACGCCACAA CCTGTCTGGG
    CGCTTTGAGT CGCGCTGGGC TAGTGTGCGC GTGGGGCCCG GGCCAGCCCT GATGCTGCGA
    GGGATGGAAG GCGCGGTGCT GCCCGTGTGG AGCGCTCACG GGGAAGGTTA CATGGCATTT
    TCTTCTCCGG AACTCCAAGC CCAGATGGAG GCCAGGGGCT TAGCTCCACT GCACTGGGCA
    GATGATGATG GGAACCCCAC GGAACAGTAC CCCCTGAATC CCAACGGCTC CCCAGGGGGT
    GTCGCTGGCA TCTGCTCCCC CGATGGCCGC CACTTGGCCC TCATGCCTCA CCCGGAGCGG
    TCTGTGAGGC CTTGGCAGTG GGCCTGGCGA CCTCCTCCAT TTGACACTCT AACCACCTCC
    CCCTGGCTCC AGCTCTTCAT CAATGCCCGG AATTGGACCC AGGAATGCAG CTGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024835935.1
    CDS80..4096
    Translation

    Target ORF information:

    RefSeq Version XM_024980167.1
    Organism Bos taurus(cattle)
    Definition Bos taurus phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024980167.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTCCTCCG TCCTTCACTT CTATGTCCGC CCCTCTGGCC ACGAGAGGGC AGCCTCTGAA 
    TACATTCAAA GGAAGCTGCA AAGGGAACTG CCAGAGCTGC AGGGTGTCAA GACCGAGCAG
    TGCTACAATG TGAACTGGAC AGCGGAGTCC TTCCCGAGCA ACAAGGAGAT GAAGAAGCTG
    ACATGGCTAT TTGGCTGCCC CTTGTTACTG GACGATGTTG CTCAGGAATC CTGGCTTCGT
    CCTGGCCCCA CAGACCTGCT GCTGGAGGTC GGACCCAGGC TGAACTTTTC CACCCCGACA
    TCCAGCAACA TTGTGTCAGT GTGCCAGGCT GCAGGGCTGG GGGCTGTGGA CCGCGTGGAG
    CCAACCCGGC GCTACCTGCT CTCGTTTGCC CACCCACCTT CAGCTGACCT GGAGGCCATT
    GCCCTGGCTA CCCTGCATGA CCGGATGACA GAGCAGCGCT TCCCCCAGCC CATCCAGAGC
    TTCTCTTCAG GGTGCATCCC AGCACCCCTG AGCGGCCCAA TTGACGTGCT GGCTGAGGGC
    CGGTCTGCCC TGGAGAAAGC CAACCAGGAG CTAGGTCTGG CCCTAGACTC CTGGGACCTG
    GATTTCTACA CCAAGCGCTT CCAGGAGCTG CAGCGCAACC CCAGCACTGT GGAAGTCTTC
    GACTTGGCTC AGTCCAATAG CGAACACAGC CGACACTGGT TCTTCAAGGG TCGACTCCAT
    GTGGATGGAC AGGAGCTGCC ACACTCGCTG TTTGAGTCCA TCATGAGCAC CCAGGCGTCC
    TCCAACCCTA ACAACGTCCT CAAGTTCTGT GACAACAGCA GTGCCATCCA GGGGAAAGAA
    GTCCGATTCT TGCGGCCTAA GGACCCCACA CGGCCAAGCT CCTTCCAGCA ATGTCAGGGG
    CTGAGACATG TTGTCTTCAC AGCAGAGACA CACAACTTCC CTACAGGAGT AGCCCCCTTC
    AGTGGTGCAA CCACTGGCAC AGGGGGCCGG ATCCGAGATG TCCAGTGCAC AGGCCGAGGG
    GCCCACGTGG TGGCTGGCAC TGCTGGCTAT TGCTTTGGAA ACCTGCACAT CCCAGGTTAC
    AGTATGCCCT GGGAGGATCC AAGCTTCCAG TATCCAGGAA ACTTTGCCCG ACCCCTGGAG
    ATTGCCATTG AGGCCAGTAA TGGGGCTTCT GACTACGGCA ACAAGTTTGG GGAACCAGTG
    CTTGCTGGCT TTGCCCGCTC CTTGGGCCTT CAGCTCCCGG ACGGCCAGCG GCGTGAGTGG
    ATCAAGCCCA TCATGTTTAG TGGAGGCATT GGGTCCATGG AAGCTGATCA TGTGAGCAAG
    GAGCCCCCAG AGACAGGCAT GGATGTCGTG AAGATTGGCG GTCCTGTCTA CAGGATTGGA
    GTTGGGGGCG GAGCTGCTTC GTCTGTGCAG GTGCAAGGAG ACAACGCCAG TGACCTGGAT
    TTTGGGGCTG TGCAGCGGGG AGACCCAGAA ATGGAGCAGA AGATGAACCG TGTGATCCGG
    GCTTGTGTGG AGGCCCCTGG GGGAAACCCC ATCTGCAGCC TCCATGACCA GGGTGCTGGT
    GGCAATGGCA ATGTCCTGAA GGAGCTGAGT GACCCGGCTG GAGCCATCAT TCACACCAGC
    TGCTTCCAGC TGGGGGACCC AACCCTGAAT GCCCTGGAAA TCTGGGGGGC CGAGTACCAG
    GAGTCGAACG CACTTCTGCT GAGGCCCTCT GACCGGGACT TCCTGAGTTG TGTCAGCGCC
    CGGGAACGCT GCCCAGTCTG CTTTGTGGGC ACCATCACTG GAGACAGGAG AATAGTGCTG
    GTGGATGATC GGGAGTGGCC TATGGGCACA GGTGGCCAGG TGGATGCGCC CCCCACTCCT
    CCCCCAACCC CTGTGGACCT GGACCTGGAC TGGGTGCTGG GCAAGATGCC CCAGAAGGAG
    TTCTTCCTCC AGAGGAGTCT CCCCTTGCTG CAGCCTCTGA CTCTGCCCCC AGGGCTGAGC
    GTGCGCCAGG CTCTGGCGCG GGTCCTCAGG CTGCCTGCCG TGGCCAGCAA GCGCTACCTC
    ACCAACAAGG TGGACCGTTC TGTGGGCGGC CTGGTGGCCC AGCAGCAGTG TGTGGGACCC
    CTGCAGACCC CTCTGGCAGA TGTGGCGGTT GTGGCACTGA GTCACCAGGA GCTTGTAGGG
    GCCGCCACAG CCCTGGGAGA GCAGCCAGTC AAGAGCCTGC TGGACCCGAA GGTTGCCGCC
    CGGCTGGCTG TGGCCGAAGC CCTCACCAAC CTGGTATTTG CTCTGGTCAC CGACCTCCAG
    GATGTGAAAT GCAGCGGGAA CTGGATGTGG GCAGCCAAGC TCCCAGGGGA GGGTGCAGCT
    CTGGCTGACG CCTGTGAGGC TATGGTGGCA GTGATGGCGG CCCTGGGTGT AGCGGTAGAC
    GGTGGCAAGG ACTCCCTCAG CATGGCTGCC CGGGTTGGCT CTGAAACCGT GCGGGCTCCT
    GGGTCACTGG TCATCTCAGC CTATGCTGTC TGTTCAGACA TTACAGCCAC TGTGACCCCA
    GACCTCAAGC GTCCTGGGGG GAGAGGCCAC CTGCTCTACG TGCCTCTGAG TCCTGGGCAG
    CACCGGCTGG GGGGCACAGC TCTGGCTCAG TGCTTCTCCC AGCTTGGCGA GCAGCCTCCC
    GACCTGGATG TTCCTGAGAA CTTGGTGCGA GCCTTCAGCA TCACCCAGGG GCTGCTGAAA
    GACCGCCTCC TCTGCTCAGG CCATGATGTC AGTGATGGAG GTCTCATCAC TTGCCTGCTA
    GAGATGGCCT TTGCTGGGAA TTGTGGGATA GAGGTGGACA TCCCCGCACC TGGGGTCGAT
    GCCCTGCCTG TGCTGTTCGC CGAGGAGCCA GGCCTGGTGC TGGAGGTGCA GGAGCCGGAC
    CTCGCCCAAG TGCTGATGCG TTACCGGAAT GCTGGCCTCC ACTGCCTAGA GCTGGGTCTC
    ACAGGCGACA CGGGGCCCCA CGCCATGGTC CGCGTGTCGG TGAACAGGAC GGTGGTTCTG
    GAGGAACCTG TTGGGCAGCT ACGAGCCCTC TGGGAGGAAA CCAGTTTCCA GTTGGACCGG
    CTACAGGCAA AACCACACTG TGTGGCCCAG GAAGAGCAAG GGCTGAGGGA GCGGGCAGGG
    CCCACCTTCT GCCTGCCGCC TACCTTCCCC AGAGCTTCCG TGCCTTGTGA GCCTGGTGGT
    CCTGCCCCCC GAGTCGCCAT CATTCGGGAA GAGGGCAGTA ATGGAGACCG GGAGATGGCA
    GATGCCTTCC ACTTGGCTGG ATTTGAGGTG TGGGATGTAA CCATGCAGGA CCTGTGCTCT
    GGAGCCATCG GGCTGGACAC ATTCCGCGGT GTGGCCTTCG TAGGCGGCTT CAGCTACGCG
    GACGTCCTGG GCTCTGCCAA AGGCTGGGCA GCTGCTGTGA CCTTTAACCC GCAGGCCGGG
    GCTGAGATGA AGCGCTTCCG CCAGCGGCCA GACACCTTCA GCCTGGGCGT GTGTAACGGC
    TGTCAGCTGC TGGCCCTGCT GGGCTGGGTG GGAGGCAGTT CTAGTGAGGA GGTGGTGGAG
    ATGTGCCAGG AGTCCTGGCC GGCCCGGCCC GGTCTCCTGC TACGCCACAA CCTGTCTGGG
    CGCTTTGAGT CGCGCTGGGC TAGTGTGCGC GTGGGGCCCG GGCCAGCCCT GATGCTGCGA
    GGGATGGAAG GCGCGGTGCT GCCCGTGTGG AGCGCTCACG GGGAAGGTTA CATGGCATTT
    TCTTCTCCGG AACTCCAAGC CCAGATGGAG GCCAGGGGCT TAGCTCCACT GCACTGGGCA
    GATGATGATG GGAACCCCAC GGAACAGTAC CCCCTGAATC CCAACGGCTC CCCAGGGGGT
    GTCGCTGGCA TCTGCTCCCC CGATGGCCGC CACTTGGCCC TCATGCCTCA CCCGGAGCGG
    TCTGTGAGGC CTTGGCAGTG GGCCTGGCGA CCTCCTCCAT TTGACACTCT AACCACCTCC
    CCCTGGCTCC AGCTCTTCAT CAATGCCCGG AATTGGACCC AGGAATGCAG CTGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa226375
    Clone ID Related Accession (Same CDS sequence) XM_024980167.1 , XM_024980168.1
    Accession Version XM_024980168.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4017bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product phosphoribosylformylglycinamidine synthase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTCCTCCG TCCTTCACTT CTATGTCCGC CCCTCTGGCC ACGAGAGGGC AGCCTCTGAA 
    TACATTCAAA GGAAGCTGCA AAGGGAACTG CCAGAGCTGC AGGGTGTCAA GACCGAGCAG
    TGCTACAATG TGAACTGGAC AGCGGAGTCC TTCCCGAGCA ACAAGGAGAT GAAGAAGCTG
    ACATGGCTAT TTGGCTGCCC CTTGTTACTG GACGATGTTG CTCAGGAATC CTGGCTTCGT
    CCTGGCCCCA CAGACCTGCT GCTGGAGGTC GGACCCAGGC TGAACTTTTC CACCCCGACA
    TCCAGCAACA TTGTGTCAGT GTGCCAGGCT GCAGGGCTGG GGGCTGTGGA CCGCGTGGAG
    CCAACCCGGC GCTACCTGCT CTCGTTTGCC CACCCACCTT CAGCTGACCT GGAGGCCATT
    GCCCTGGCTA CCCTGCATGA CCGGATGACA GAGCAGCGCT TCCCCCAGCC CATCCAGAGC
    TTCTCTTCAG GGTGCATCCC AGCACCCCTG AGCGGCCCAA TTGACGTGCT GGCTGAGGGC
    CGGTCTGCCC TGGAGAAAGC CAACCAGGAG CTAGGTCTGG CCCTAGACTC CTGGGACCTG
    GATTTCTACA CCAAGCGCTT CCAGGAGCTG CAGCGCAACC CCAGCACTGT GGAAGTCTTC
    GACTTGGCTC AGTCCAATAG CGAACACAGC CGACACTGGT TCTTCAAGGG TCGACTCCAT
    GTGGATGGAC AGGAGCTGCC ACACTCGCTG TTTGAGTCCA TCATGAGCAC CCAGGCGTCC
    TCCAACCCTA ACAACGTCCT CAAGTTCTGT GACAACAGCA GTGCCATCCA GGGGAAAGAA
    GTCCGATTCT TGCGGCCTAA GGACCCCACA CGGCCAAGCT CCTTCCAGCA ATGTCAGGGG
    CTGAGACATG TTGTCTTCAC AGCAGAGACA CACAACTTCC CTACAGGAGT AGCCCCCTTC
    AGTGGTGCAA CCACTGGCAC AGGGGGCCGG ATCCGAGATG TCCAGTGCAC AGGCCGAGGG
    GCCCACGTGG TGGCTGGCAC TGCTGGCTAT TGCTTTGGAA ACCTGCACAT CCCAGGTTAC
    AGTATGCCCT GGGAGGATCC AAGCTTCCAG TATCCAGGAA ACTTTGCCCG ACCCCTGGAG
    ATTGCCATTG AGGCCAGTAA TGGGGCTTCT GACTACGGCA ACAAGTTTGG GGAACCAGTG
    CTTGCTGGCT TTGCCCGCTC CTTGGGCCTT CAGCTCCCGG ACGGCCAGCG GCGTGAGTGG
    ATCAAGCCCA TCATGTTTAG TGGAGGCATT GGGTCCATGG AAGCTGATCA TGTGAGCAAG
    GAGCCCCCAG AGACAGGCAT GGATGTCGTG AAGATTGGCG GTCCTGTCTA CAGGATTGGA
    GTTGGGGGCG GAGCTGCTTC GTCTGTGCAG GTGCAAGGAG ACAACGCCAG TGACCTGGAT
    TTTGGGGCTG TGCAGCGGGG AGACCCAGAA ATGGAGCAGA AGATGAACCG TGTGATCCGG
    GCTTGTGTGG AGGCCCCTGG GGGAAACCCC ATCTGCAGCC TCCATGACCA GGGTGCTGGT
    GGCAATGGCA ATGTCCTGAA GGAGCTGAGT GACCCGGCTG GAGCCATCAT TCACACCAGC
    TGCTTCCAGC TGGGGGACCC AACCCTGAAT GCCCTGGAAA TCTGGGGGGC CGAGTACCAG
    GAGTCGAACG CACTTCTGCT GAGGCCCTCT GACCGGGACT TCCTGAGTTG TGTCAGCGCC
    CGGGAACGCT GCCCAGTCTG CTTTGTGGGC ACCATCACTG GAGACAGGAG AATAGTGCTG
    GTGGATGATC GGGAGTGGCC TATGGGCACA GGTGGCCAGG TGGATGCGCC CCCCACTCCT
    CCCCCAACCC CTGTGGACCT GGACCTGGAC TGGGTGCTGG GCAAGATGCC CCAGAAGGAG
    TTCTTCCTCC AGAGGAGTCT CCCCTTGCTG CAGCCTCTGA CTCTGCCCCC AGGGCTGAGC
    GTGCGCCAGG CTCTGGCGCG GGTCCTCAGG CTGCCTGCCG TGGCCAGCAA GCGCTACCTC
    ACCAACAAGG TGGACCGTTC TGTGGGCGGC CTGGTGGCCC AGCAGCAGTG TGTGGGACCC
    CTGCAGACCC CTCTGGCAGA TGTGGCGGTT GTGGCACTGA GTCACCAGGA GCTTGTAGGG
    GCCGCCACAG CCCTGGGAGA GCAGCCAGTC AAGAGCCTGC TGGACCCGAA GGTTGCCGCC
    CGGCTGGCTG TGGCCGAAGC CCTCACCAAC CTGGTATTTG CTCTGGTCAC CGACCTCCAG
    GATGTGAAAT GCAGCGGGAA CTGGATGTGG GCAGCCAAGC TCCCAGGGGA GGGTGCAGCT
    CTGGCTGACG CCTGTGAGGC TATGGTGGCA GTGATGGCGG CCCTGGGTGT AGCGGTAGAC
    GGTGGCAAGG ACTCCCTCAG CATGGCTGCC CGGGTTGGCT CTGAAACCGT GCGGGCTCCT
    GGGTCACTGG TCATCTCAGC CTATGCTGTC TGTTCAGACA TTACAGCCAC TGTGACCCCA
    GACCTCAAGC GTCCTGGGGG GAGAGGCCAC CTGCTCTACG TGCCTCTGAG TCCTGGGCAG
    CACCGGCTGG GGGGCACAGC TCTGGCTCAG TGCTTCTCCC AGCTTGGCGA GCAGCCTCCC
    GACCTGGATG TTCCTGAGAA CTTGGTGCGA GCCTTCAGCA TCACCCAGGG GCTGCTGAAA
    GACCGCCTCC TCTGCTCAGG CCATGATGTC AGTGATGGAG GTCTCATCAC TTGCCTGCTA
    GAGATGGCCT TTGCTGGGAA TTGTGGGATA GAGGTGGACA TCCCCGCACC TGGGGTCGAT
    GCCCTGCCTG TGCTGTTCGC CGAGGAGCCA GGCCTGGTGC TGGAGGTGCA GGAGCCGGAC
    CTCGCCCAAG TGCTGATGCG TTACCGGAAT GCTGGCCTCC ACTGCCTAGA GCTGGGTCTC
    ACAGGCGACA CGGGGCCCCA CGCCATGGTC CGCGTGTCGG TGAACAGGAC GGTGGTTCTG
    GAGGAACCTG TTGGGCAGCT ACGAGCCCTC TGGGAGGAAA CCAGTTTCCA GTTGGACCGG
    CTACAGGCAA AACCACACTG TGTGGCCCAG GAAGAGCAAG GGCTGAGGGA GCGGGCAGGG
    CCCACCTTCT GCCTGCCGCC TACCTTCCCC AGAGCTTCCG TGCCTTGTGA GCCTGGTGGT
    CCTGCCCCCC GAGTCGCCAT CATTCGGGAA GAGGGCAGTA ATGGAGACCG GGAGATGGCA
    GATGCCTTCC ACTTGGCTGG ATTTGAGGTG TGGGATGTAA CCATGCAGGA CCTGTGCTCT
    GGAGCCATCG GGCTGGACAC ATTCCGCGGT GTGGCCTTCG TAGGCGGCTT CAGCTACGCG
    GACGTCCTGG GCTCTGCCAA AGGCTGGGCA GCTGCTGTGA CCTTTAACCC GCAGGCCGGG
    GCTGAGATGA AGCGCTTCCG CCAGCGGCCA GACACCTTCA GCCTGGGCGT GTGTAACGGC
    TGTCAGCTGC TGGCCCTGCT GGGCTGGGTG GGAGGCAGTT CTAGTGAGGA GGTGGTGGAG
    ATGTGCCAGG AGTCCTGGCC GGCCCGGCCC GGTCTCCTGC TACGCCACAA CCTGTCTGGG
    CGCTTTGAGT CGCGCTGGGC TAGTGTGCGC GTGGGGCCCG GGCCAGCCCT GATGCTGCGA
    GGGATGGAAG GCGCGGTGCT GCCCGTGTGG AGCGCTCACG GGGAAGGTTA CATGGCATTT
    TCTTCTCCGG AACTCCAAGC CCAGATGGAG GCCAGGGGCT TAGCTCCACT GCACTGGGCA
    GATGATGATG GGAACCCCAC GGAACAGTAC CCCCTGAATC CCAACGGCTC CCCAGGGGGT
    GTCGCTGGCA TCTGCTCCCC CGATGGCCGC CACTTGGCCC TCATGCCTCA CCCGGAGCGG
    TCTGTGAGGC CTTGGCAGTG GGCCTGGCGA CCTCCTCCAT TTGACACTCT AACCACCTCC
    CCCTGGCTCC AGCTCTTCAT CAATGCCCGG AATTGGACCC AGGAATGCAG CTGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024835936.1
    CDS206..4222
    Translation

    Target ORF information:

    RefSeq Version XM_024980168.1
    Organism Bos taurus(cattle)
    Definition Bos taurus phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024980168.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTCCTCCG TCCTTCACTT CTATGTCCGC CCCTCTGGCC ACGAGAGGGC AGCCTCTGAA 
    TACATTCAAA GGAAGCTGCA AAGGGAACTG CCAGAGCTGC AGGGTGTCAA GACCGAGCAG
    TGCTACAATG TGAACTGGAC AGCGGAGTCC TTCCCGAGCA ACAAGGAGAT GAAGAAGCTG
    ACATGGCTAT TTGGCTGCCC CTTGTTACTG GACGATGTTG CTCAGGAATC CTGGCTTCGT
    CCTGGCCCCA CAGACCTGCT GCTGGAGGTC GGACCCAGGC TGAACTTTTC CACCCCGACA
    TCCAGCAACA TTGTGTCAGT GTGCCAGGCT GCAGGGCTGG GGGCTGTGGA CCGCGTGGAG
    CCAACCCGGC GCTACCTGCT CTCGTTTGCC CACCCACCTT CAGCTGACCT GGAGGCCATT
    GCCCTGGCTA CCCTGCATGA CCGGATGACA GAGCAGCGCT TCCCCCAGCC CATCCAGAGC
    TTCTCTTCAG GGTGCATCCC AGCACCCCTG AGCGGCCCAA TTGACGTGCT GGCTGAGGGC
    CGGTCTGCCC TGGAGAAAGC CAACCAGGAG CTAGGTCTGG CCCTAGACTC CTGGGACCTG
    GATTTCTACA CCAAGCGCTT CCAGGAGCTG CAGCGCAACC CCAGCACTGT GGAAGTCTTC
    GACTTGGCTC AGTCCAATAG CGAACACAGC CGACACTGGT TCTTCAAGGG TCGACTCCAT
    GTGGATGGAC AGGAGCTGCC ACACTCGCTG TTTGAGTCCA TCATGAGCAC CCAGGCGTCC
    TCCAACCCTA ACAACGTCCT CAAGTTCTGT GACAACAGCA GTGCCATCCA GGGGAAAGAA
    GTCCGATTCT TGCGGCCTAA GGACCCCACA CGGCCAAGCT CCTTCCAGCA ATGTCAGGGG
    CTGAGACATG TTGTCTTCAC AGCAGAGACA CACAACTTCC CTACAGGAGT AGCCCCCTTC
    AGTGGTGCAA CCACTGGCAC AGGGGGCCGG ATCCGAGATG TCCAGTGCAC AGGCCGAGGG
    GCCCACGTGG TGGCTGGCAC TGCTGGCTAT TGCTTTGGAA ACCTGCACAT CCCAGGTTAC
    AGTATGCCCT GGGAGGATCC AAGCTTCCAG TATCCAGGAA ACTTTGCCCG ACCCCTGGAG
    ATTGCCATTG AGGCCAGTAA TGGGGCTTCT GACTACGGCA ACAAGTTTGG GGAACCAGTG
    CTTGCTGGCT TTGCCCGCTC CTTGGGCCTT CAGCTCCCGG ACGGCCAGCG GCGTGAGTGG
    ATCAAGCCCA TCATGTTTAG TGGAGGCATT GGGTCCATGG AAGCTGATCA TGTGAGCAAG
    GAGCCCCCAG AGACAGGCAT GGATGTCGTG AAGATTGGCG GTCCTGTCTA CAGGATTGGA
    GTTGGGGGCG GAGCTGCTTC GTCTGTGCAG GTGCAAGGAG ACAACGCCAG TGACCTGGAT
    TTTGGGGCTG TGCAGCGGGG AGACCCAGAA ATGGAGCAGA AGATGAACCG TGTGATCCGG
    GCTTGTGTGG AGGCCCCTGG GGGAAACCCC ATCTGCAGCC TCCATGACCA GGGTGCTGGT
    GGCAATGGCA ATGTCCTGAA GGAGCTGAGT GACCCGGCTG GAGCCATCAT TCACACCAGC
    TGCTTCCAGC TGGGGGACCC AACCCTGAAT GCCCTGGAAA TCTGGGGGGC CGAGTACCAG
    GAGTCGAACG CACTTCTGCT GAGGCCCTCT GACCGGGACT TCCTGAGTTG TGTCAGCGCC
    CGGGAACGCT GCCCAGTCTG CTTTGTGGGC ACCATCACTG GAGACAGGAG AATAGTGCTG
    GTGGATGATC GGGAGTGGCC TATGGGCACA GGTGGCCAGG TGGATGCGCC CCCCACTCCT
    CCCCCAACCC CTGTGGACCT GGACCTGGAC TGGGTGCTGG GCAAGATGCC CCAGAAGGAG
    TTCTTCCTCC AGAGGAGTCT CCCCTTGCTG CAGCCTCTGA CTCTGCCCCC AGGGCTGAGC
    GTGCGCCAGG CTCTGGCGCG GGTCCTCAGG CTGCCTGCCG TGGCCAGCAA GCGCTACCTC
    ACCAACAAGG TGGACCGTTC TGTGGGCGGC CTGGTGGCCC AGCAGCAGTG TGTGGGACCC
    CTGCAGACCC CTCTGGCAGA TGTGGCGGTT GTGGCACTGA GTCACCAGGA GCTTGTAGGG
    GCCGCCACAG CCCTGGGAGA GCAGCCAGTC AAGAGCCTGC TGGACCCGAA GGTTGCCGCC
    CGGCTGGCTG TGGCCGAAGC CCTCACCAAC CTGGTATTTG CTCTGGTCAC CGACCTCCAG
    GATGTGAAAT GCAGCGGGAA CTGGATGTGG GCAGCCAAGC TCCCAGGGGA GGGTGCAGCT
    CTGGCTGACG CCTGTGAGGC TATGGTGGCA GTGATGGCGG CCCTGGGTGT AGCGGTAGAC
    GGTGGCAAGG ACTCCCTCAG CATGGCTGCC CGGGTTGGCT CTGAAACCGT GCGGGCTCCT
    GGGTCACTGG TCATCTCAGC CTATGCTGTC TGTTCAGACA TTACAGCCAC TGTGACCCCA
    GACCTCAAGC GTCCTGGGGG GAGAGGCCAC CTGCTCTACG TGCCTCTGAG TCCTGGGCAG
    CACCGGCTGG GGGGCACAGC TCTGGCTCAG TGCTTCTCCC AGCTTGGCGA GCAGCCTCCC
    GACCTGGATG TTCCTGAGAA CTTGGTGCGA GCCTTCAGCA TCACCCAGGG GCTGCTGAAA
    GACCGCCTCC TCTGCTCAGG CCATGATGTC AGTGATGGAG GTCTCATCAC TTGCCTGCTA
    GAGATGGCCT TTGCTGGGAA TTGTGGGATA GAGGTGGACA TCCCCGCACC TGGGGTCGAT
    GCCCTGCCTG TGCTGTTCGC CGAGGAGCCA GGCCTGGTGC TGGAGGTGCA GGAGCCGGAC
    CTCGCCCAAG TGCTGATGCG TTACCGGAAT GCTGGCCTCC ACTGCCTAGA GCTGGGTCTC
    ACAGGCGACA CGGGGCCCCA CGCCATGGTC CGCGTGTCGG TGAACAGGAC GGTGGTTCTG
    GAGGAACCTG TTGGGCAGCT ACGAGCCCTC TGGGAGGAAA CCAGTTTCCA GTTGGACCGG
    CTACAGGCAA AACCACACTG TGTGGCCCAG GAAGAGCAAG GGCTGAGGGA GCGGGCAGGG
    CCCACCTTCT GCCTGCCGCC TACCTTCCCC AGAGCTTCCG TGCCTTGTGA GCCTGGTGGT
    CCTGCCCCCC GAGTCGCCAT CATTCGGGAA GAGGGCAGTA ATGGAGACCG GGAGATGGCA
    GATGCCTTCC ACTTGGCTGG ATTTGAGGTG TGGGATGTAA CCATGCAGGA CCTGTGCTCT
    GGAGCCATCG GGCTGGACAC ATTCCGCGGT GTGGCCTTCG TAGGCGGCTT CAGCTACGCG
    GACGTCCTGG GCTCTGCCAA AGGCTGGGCA GCTGCTGTGA CCTTTAACCC GCAGGCCGGG
    GCTGAGATGA AGCGCTTCCG CCAGCGGCCA GACACCTTCA GCCTGGGCGT GTGTAACGGC
    TGTCAGCTGC TGGCCCTGCT GGGCTGGGTG GGAGGCAGTT CTAGTGAGGA GGTGGTGGAG
    ATGTGCCAGG AGTCCTGGCC GGCCCGGCCC GGTCTCCTGC TACGCCACAA CCTGTCTGGG
    CGCTTTGAGT CGCGCTGGGC TAGTGTGCGC GTGGGGCCCG GGCCAGCCCT GATGCTGCGA
    GGGATGGAAG GCGCGGTGCT GCCCGTGTGG AGCGCTCACG GGGAAGGTTA CATGGCATTT
    TCTTCTCCGG AACTCCAAGC CCAGATGGAG GCCAGGGGCT TAGCTCCACT GCACTGGGCA
    GATGATGATG GGAACCCCAC GGAACAGTAC CCCCTGAATC CCAACGGCTC CCCAGGGGGT
    GTCGCTGGCA TCTGCTCCCC CGATGGCCGC CACTTGGCCC TCATGCCTCA CCCGGAGCGG
    TCTGTGAGGC CTTGGCAGTG GGCCTGGCGA CCTCCTCCAT TTGACACTCT AACCACCTCC
    CCCTGGCTCC AGCTCTTCAT CAATGCCCGG AATTGGACCC AGGAATGCAG CTGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa226376
    Clone ID Related Accession (Same CDS sequence) XM_015458726.2
    Accession Version XM_015458726.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4008bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product phosphoribosylformylglycinamidine synthase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015458726.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTCCTCCG TCCTTCACTT CTATGTCCGC CCCTCTGGCC ACGAGAGGGC AGCCTCTGAA 
    TACATTCAAA GGAAGCTGCA AAGGGAACTG CCAGAGCTGC AGGGTGTCAA GACCGAGCAG
    TGCTACAATG TGAACTGGAC AGCGGAGTCC TTCCCGAGCA ACAAGGAGAT GAAGAAGCTG
    ACATGGCTAT TTGGCTGCCC CTTGTTACTG GACGATGTTG CTCAGGAATC CTGGCTTCGT
    CCTGGCCCCA CAGACCTGCT GCTGGAGGTC GGACCCAGGC TGAACTTTTC CACCCCGACA
    TCCAGCAACA TTGTGTCAGT GTGCCAGGCT GCAGGGCTGG GGGCTGTGGA CCGCGTGGAG
    CCAACCCGGC GCTACCTGCT CTCGTTTGCC CACCCACCTT CAGCTGACCT GGAGGCCATT
    GCCCTGGCTA CCCTGCATGA CCGGATGACA GAGCAGCGCT TCCCCCAGCC CATCCAGAGC
    TTCTCTTCAG GGTGCATCCC AGCACCCCTG AGCGGCCCAA TTGACGTGCT GGCTGAGGGC
    CGGTCTGCCC TGGAGAAAGC CAACCAGGAG CTAGGTCTGG CCCTAGACTC CTGGGACCTG
    GATTTCTACA CCAAGCGCTT CCAGGAGCTG CAGCGCAACC CCAGCACTGT GGAAGTCTTC
    GACTTGGCTC AGTCCAATAG CGAACACAGC CGACACTGGT TCTTCAAGGG TCGACTCCAT
    GTGGATGGAC AGGAGCTGCC ACACTCGCTG TTTGAGTCCA TCATGAGCAC CCAGGCGTCC
    TCCAACCCTA ACAACGTCCT CAAGTTCTGT GACAACAGCA GTGCCATCCA GGGGAAAGAA
    GTCCGATTCT TGCGGCCTAA GGACCCCACA CGGCCAAGCT CCTTCCAGCA ATGTCAGGGG
    CTGAGACATG TTGTCTTCAC AGCAGAGACA CACAACTTCC CTACAGGAGT AGCCCCCTTC
    AGTGGTGCAA CCACTGGCAC AGGGGGCCGG ATCCGAGATG TCCAGTGCAC AGGCCGAGGG
    GCCCACGTGG TGGCTGGCAC TGCTGGCTAT TGCTTTGGAA ACCTGCACAT CCCAGGTTAC
    AGTATGCCCT GGGAGGATCC AAGCTTCCAG TATCCAGGAA ACTTTGCCCG ACCCCTGGAG
    ATTGCCATTG AGGCCAGTAA TGGGGCTTCT GACTACGGCA ACAAGTTTGG GGAACCAGTG
    CTTGCTGGCT TTGCCCGCTC CTTGGGCCTT CAGCTCCCGG ACGGCCAGCG GCGTGAGTGG
    ATCAAGCCCA TCATGTTTAG TGGAGGCATT GGGTCCATGG AAGCTGATCA TGTGAGCAAG
    GAGCCCCCAG AGACAGGCAT GGATGTCGTG AAGATTGGCG GTCCTGTCTA CAGGATTGGA
    GTTGGGGGCG GAGCTGCTTC GTCTGTGCAG GTGCAAGGAG ACAACGCCAG TGACCTGGAT
    TTTGGGGCTG TGCAGCGGGG AGACCCAGAA ATGGAGCAGA AGATGAACCG TGTGATCCGG
    GCTTGTGTGG AGGCCCCTGG GGGAAACCCC ATCTGCAGCC TCCATGACCA GGGTGCTGGT
    GGCAATGGCA ATGTCCTGAA GGAGCTGAGT GACCCGGCTG GAGCCATCAT TCACACCAGC
    TGCTTCCAGC TGGGGGACCC AACCCTGAAT GCCCTGGAAA TCTGGGGGGC CGAGTACCAG
    GAGTCGAACG CACTTCTGCT GAGGCCCTCT GACCGGGACT TCCTGAGTTG TGTCAGCGCC
    CGGGAACGCT GCCCAGTCTG CTTTGTGGGC ACCATCACTG GAGACAGGAG AATAGTGCTG
    GTGGATGATC GGGAGTGGCC TATGGGCACA GGTGGCCAGG TGGATGCGCC CCCCACTCCT
    CCCCCAACCC CTGTGGACCT GGACCTGGAC TGGGTGCTGG GCAAGATGCC CCAGAAGGAG
    TTCTTCCTCC AGAGGAGTCT CCCCTTGCTG CAGCCTCTGA CTCTGCCCCC AGGGCTGAGC
    GTGCGCCAGG CTCTGGCGCG GGTCCTCAGG CTGCCTGCCG TGGCCAGCAA GCGCTACCTC
    ACCAACAAGG TGGACCGTTC TGTGGGCGGC CTGGTGGCCC AGCAGCAGTG TGTGGGACCC
    CTGCAGACCC CTCTGGCAGA TGTGGCGGTT GTGGCACTGA GTCACCAGGA GCTTGTAGGG
    GCCGCCACAG CCCTGGGAGA GCAGCCAGTC AAGAGCCTGC TGGACCCGAA GGTTGCCGCC
    CGGCTGGCTG TGGCCGAAGC CCTCACCAAC CTGGTATTTG CTCTGGTCAC CGACCTCCAG
    GATGTGAAAT GCAGCGGGAA CTGGATGTGG GCAGCCAAGC TCCCAGGGGA GGGTGCAGCT
    CTGGCTGACG CCTGTGAGGC TATGGTGGCA GTGATGGCGG CCCTGGGTGT AGCGGTAGAC
    GGTGGCAAGG ACTCCCTCAG CATGGCTGCC CGGGTTGGCT CTGAAACCGT GCGGGCTCCT
    GGGTCACTGG TCATCTCAGC CTATGCTGTC TGTTCAGACA TTACAGCCAC TGTGACCCCA
    GACCTCAAGC GTCCTGGGGG GAGAGGCCAC CTGCTCTACG TGCCTCTGAG TCCTGGGCAG
    CACCGGCTGG GGGGCACAGC TCTGGCTCAG TGCTTCTCCC AGCTTGGCGA GCAGCCTCCC
    GACCTGGATG TTCCTGAGAA CTTGGTGCGA GCCTTCAGCA TCACCCAGGG GCTGCTGAAA
    GACCGCCTCC TCTGCTCAGG CCATGATGTC AGTGATGGAG GTCTCATCAC TTGCCTGCTA
    GAGATGGCCT TTGCTGGGAA TTGTGGGATA GAGGTGGACA TCCCCGCACC TGGGGTCGAT
    GCCCTGCCTG TGCTGTTCGC CGAGGAGCCA GGCCTGGTGC TGGAGGTGCA GGAGCCGGAC
    CTCGCCCAAG TGCTGATGCG TTACCGGAAT GCTGGCCTCC ACTGCCTAGA GCTGGGTCTC
    ACAGGCGACA CGGGGCCCCA CGCCATGGTC CGCGTGTCGG TGAACAGGAC GGTGGTTCTG
    GAGGAACCTG TTGGGCAGCT ACGAGCCCTC TGGGAGGAAA CCAGTTTCCA GTTGGACCGG
    CTACAGGCAA AACCACACTG TGTGGCCCAG GAAGAGCAAG GGCTGAGGGA GCGGGCAGGG
    CCCACCTTCT GCCTGCCGCC TACCTTCCCC AGAGCTTCCG TGCCTTGTGG TCCTGCCCCC
    CGAGTCGCCA TCATTCGGGA AGAGGGCAGT AATGGAGACC GGGAGATGGC AGATGCCTTC
    CACTTGGCTG GATTTGAGGT GTGGGATGTA ACCATGCAGG ACCTGTGCTC TGGAGCCATC
    GGGCTGGACA CATTCCGCGG TGTGGCCTTC GTAGGCGGCT TCAGCTACGC GGACGTCCTG
    GGCTCTGCCA AAGGCTGGGC AGCTGCTGTG ACCTTTAACC CGCAGGCCGG GGCTGAGATG
    AAGCGCTTCC GCCAGCGGCC AGACACCTTC AGCCTGGGCG TGTGTAACGG CTGTCAGCTG
    CTGGCCCTGC TGGGCTGGGT GGGAGGCAGT TCTAGTGAGG AGGTGGTGGA GATGTGCCAG
    GAGTCCTGGC CGGCCCGGCC CGGTCTCCTG CTACGCCACA ACCTGTCTGG GCGCTTTGAG
    TCGCGCTGGG CTAGTGTGCG CGTGGGGCCC GGGCCAGCCC TGATGCTGCG AGGGATGGAA
    GGCGCGGTGC TGCCCGTGTG GAGCGCTCAC GGGGAAGGTT ACATGGCATT TTCTTCTCCG
    GAACTCCAAG CCCAGATGGA GGCCAGGGGC TTAGCTCCAC TGCACTGGGC AGATGATGAT
    GGGAACCCCA CGGAACAGTA CCCCCTGAAT CCCAACGGCT CCCCAGGGGG TGTCGCTGGC
    ATCTGCTCCC CCGATGGCCG CCACTTGGCC CTCATGCCTC ACCCGGAGCG GTCTGTGAGG
    CCTTGGCAGT GGGCCTGGCG ACCTCCTCCA TTTGACACTC TAACCACCTC CCCCTGGCTC
    CAGCTCTTCA TCAATGCCCG GAATTGGACC CAGGAATGCA GCTGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015314212.2
    CDS81..4088
    Translation

    Target ORF information:

    RefSeq Version XM_015458726.2
    Organism Bos taurus(cattle)
    Definition Bos taurus phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015458726.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTCCTCCG TCCTTCACTT CTATGTCCGC CCCTCTGGCC ACGAGAGGGC AGCCTCTGAA 
    TACATTCAAA GGAAGCTGCA AAGGGAACTG CCAGAGCTGC AGGGTGTCAA GACCGAGCAG
    TGCTACAATG TGAACTGGAC AGCGGAGTCC TTCCCGAGCA ACAAGGAGAT GAAGAAGCTG
    ACATGGCTAT TTGGCTGCCC CTTGTTACTG GACGATGTTG CTCAGGAATC CTGGCTTCGT
    CCTGGCCCCA CAGACCTGCT GCTGGAGGTC GGACCCAGGC TGAACTTTTC CACCCCGACA
    TCCAGCAACA TTGTGTCAGT GTGCCAGGCT GCAGGGCTGG GGGCTGTGGA CCGCGTGGAG
    CCAACCCGGC GCTACCTGCT CTCGTTTGCC CACCCACCTT CAGCTGACCT GGAGGCCATT
    GCCCTGGCTA CCCTGCATGA CCGGATGACA GAGCAGCGCT TCCCCCAGCC CATCCAGAGC
    TTCTCTTCAG GGTGCATCCC AGCACCCCTG AGCGGCCCAA TTGACGTGCT GGCTGAGGGC
    CGGTCTGCCC TGGAGAAAGC CAACCAGGAG CTAGGTCTGG CCCTAGACTC CTGGGACCTG
    GATTTCTACA CCAAGCGCTT CCAGGAGCTG CAGCGCAACC CCAGCACTGT GGAAGTCTTC
    GACTTGGCTC AGTCCAATAG CGAACACAGC CGACACTGGT TCTTCAAGGG TCGACTCCAT
    GTGGATGGAC AGGAGCTGCC ACACTCGCTG TTTGAGTCCA TCATGAGCAC CCAGGCGTCC
    TCCAACCCTA ACAACGTCCT CAAGTTCTGT GACAACAGCA GTGCCATCCA GGGGAAAGAA
    GTCCGATTCT TGCGGCCTAA GGACCCCACA CGGCCAAGCT CCTTCCAGCA ATGTCAGGGG
    CTGAGACATG TTGTCTTCAC AGCAGAGACA CACAACTTCC CTACAGGAGT AGCCCCCTTC
    AGTGGTGCAA CCACTGGCAC AGGGGGCCGG ATCCGAGATG TCCAGTGCAC AGGCCGAGGG
    GCCCACGTGG TGGCTGGCAC TGCTGGCTAT TGCTTTGGAA ACCTGCACAT CCCAGGTTAC
    AGTATGCCCT GGGAGGATCC AAGCTTCCAG TATCCAGGAA ACTTTGCCCG ACCCCTGGAG
    ATTGCCATTG AGGCCAGTAA TGGGGCTTCT GACTACGGCA ACAAGTTTGG GGAACCAGTG
    CTTGCTGGCT TTGCCCGCTC CTTGGGCCTT CAGCTCCCGG ACGGCCAGCG GCGTGAGTGG
    ATCAAGCCCA TCATGTTTAG TGGAGGCATT GGGTCCATGG AAGCTGATCA TGTGAGCAAG
    GAGCCCCCAG AGACAGGCAT GGATGTCGTG AAGATTGGCG GTCCTGTCTA CAGGATTGGA
    GTTGGGGGCG GAGCTGCTTC GTCTGTGCAG GTGCAAGGAG ACAACGCCAG TGACCTGGAT
    TTTGGGGCTG TGCAGCGGGG AGACCCAGAA ATGGAGCAGA AGATGAACCG TGTGATCCGG
    GCTTGTGTGG AGGCCCCTGG GGGAAACCCC ATCTGCAGCC TCCATGACCA GGGTGCTGGT
    GGCAATGGCA ATGTCCTGAA GGAGCTGAGT GACCCGGCTG GAGCCATCAT TCACACCAGC
    TGCTTCCAGC TGGGGGACCC AACCCTGAAT GCCCTGGAAA TCTGGGGGGC CGAGTACCAG
    GAGTCGAACG CACTTCTGCT GAGGCCCTCT GACCGGGACT TCCTGAGTTG TGTCAGCGCC
    CGGGAACGCT GCCCAGTCTG CTTTGTGGGC ACCATCACTG GAGACAGGAG AATAGTGCTG
    GTGGATGATC GGGAGTGGCC TATGGGCACA GGTGGCCAGG TGGATGCGCC CCCCACTCCT
    CCCCCAACCC CTGTGGACCT GGACCTGGAC TGGGTGCTGG GCAAGATGCC CCAGAAGGAG
    TTCTTCCTCC AGAGGAGTCT CCCCTTGCTG CAGCCTCTGA CTCTGCCCCC AGGGCTGAGC
    GTGCGCCAGG CTCTGGCGCG GGTCCTCAGG CTGCCTGCCG TGGCCAGCAA GCGCTACCTC
    ACCAACAAGG TGGACCGTTC TGTGGGCGGC CTGGTGGCCC AGCAGCAGTG TGTGGGACCC
    CTGCAGACCC CTCTGGCAGA TGTGGCGGTT GTGGCACTGA GTCACCAGGA GCTTGTAGGG
    GCCGCCACAG CCCTGGGAGA GCAGCCAGTC AAGAGCCTGC TGGACCCGAA GGTTGCCGCC
    CGGCTGGCTG TGGCCGAAGC CCTCACCAAC CTGGTATTTG CTCTGGTCAC CGACCTCCAG
    GATGTGAAAT GCAGCGGGAA CTGGATGTGG GCAGCCAAGC TCCCAGGGGA GGGTGCAGCT
    CTGGCTGACG CCTGTGAGGC TATGGTGGCA GTGATGGCGG CCCTGGGTGT AGCGGTAGAC
    GGTGGCAAGG ACTCCCTCAG CATGGCTGCC CGGGTTGGCT CTGAAACCGT GCGGGCTCCT
    GGGTCACTGG TCATCTCAGC CTATGCTGTC TGTTCAGACA TTACAGCCAC TGTGACCCCA
    GACCTCAAGC GTCCTGGGGG GAGAGGCCAC CTGCTCTACG TGCCTCTGAG TCCTGGGCAG
    CACCGGCTGG GGGGCACAGC TCTGGCTCAG TGCTTCTCCC AGCTTGGCGA GCAGCCTCCC
    GACCTGGATG TTCCTGAGAA CTTGGTGCGA GCCTTCAGCA TCACCCAGGG GCTGCTGAAA
    GACCGCCTCC TCTGCTCAGG CCATGATGTC AGTGATGGAG GTCTCATCAC TTGCCTGCTA
    GAGATGGCCT TTGCTGGGAA TTGTGGGATA GAGGTGGACA TCCCCGCACC TGGGGTCGAT
    GCCCTGCCTG TGCTGTTCGC CGAGGAGCCA GGCCTGGTGC TGGAGGTGCA GGAGCCGGAC
    CTCGCCCAAG TGCTGATGCG TTACCGGAAT GCTGGCCTCC ACTGCCTAGA GCTGGGTCTC
    ACAGGCGACA CGGGGCCCCA CGCCATGGTC CGCGTGTCGG TGAACAGGAC GGTGGTTCTG
    GAGGAACCTG TTGGGCAGCT ACGAGCCCTC TGGGAGGAAA CCAGTTTCCA GTTGGACCGG
    CTACAGGCAA AACCACACTG TGTGGCCCAG GAAGAGCAAG GGCTGAGGGA GCGGGCAGGG
    CCCACCTTCT GCCTGCCGCC TACCTTCCCC AGAGCTTCCG TGCCTTGTGG TCCTGCCCCC
    CGAGTCGCCA TCATTCGGGA AGAGGGCAGT AATGGAGACC GGGAGATGGC AGATGCCTTC
    CACTTGGCTG GATTTGAGGT GTGGGATGTA ACCATGCAGG ACCTGTGCTC TGGAGCCATC
    GGGCTGGACA CATTCCGCGG TGTGGCCTTC GTAGGCGGCT TCAGCTACGC GGACGTCCTG
    GGCTCTGCCA AAGGCTGGGC AGCTGCTGTG ACCTTTAACC CGCAGGCCGG GGCTGAGATG
    AAGCGCTTCC GCCAGCGGCC AGACACCTTC AGCCTGGGCG TGTGTAACGG CTGTCAGCTG
    CTGGCCCTGC TGGGCTGGGT GGGAGGCAGT TCTAGTGAGG AGGTGGTGGA GATGTGCCAG
    GAGTCCTGGC CGGCCCGGCC CGGTCTCCTG CTACGCCACA ACCTGTCTGG GCGCTTTGAG
    TCGCGCTGGG CTAGTGTGCG CGTGGGGCCC GGGCCAGCCC TGATGCTGCG AGGGATGGAA
    GGCGCGGTGC TGCCCGTGTG GAGCGCTCAC GGGGAAGGTT ACATGGCATT TTCTTCTCCG
    GAACTCCAAG CCCAGATGGA GGCCAGGGGC TTAGCTCCAC TGCACTGGGC AGATGATGAT
    GGGAACCCCA CGGAACAGTA CCCCCTGAAT CCCAACGGCT CCCCAGGGGG TGTCGCTGGC
    ATCTGCTCCC CCGATGGCCG CCACTTGGCC CTCATGCCTC ACCCGGAGCG GTCTGTGAGG
    CCTTGGCAGT GGGCCTGGCG ACCTCCTCCA TTTGACACTC TAACCACCTC CCCCTGGCTC
    CAGCTCTTCA TCAATGCCCG GAATTGGACC CAGGAATGCA GCTGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa226377
    Clone ID Related Accession (Same CDS sequence) XM_005220359.4
    Accession Version XM_005220359.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4104bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product phosphoribosylformylglycinamidine synthase isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005220359.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAGGATGA CGTTGATCTG CGCGCCAGAA TTCGAGGAGC TGACGGACTG GGAGAAGTGC 
    TACCACGCGC AACGGACACC TGCAGAGATG TCCTCCGTCC TTCACTTCTA TGTCCGCCCC
    TCTGGCCACG AGAGGGCAGC CTCTGAATAC ATTCAAAGGA AGCTGCAAAG GGAACTGCCA
    GAGCTGCAGG GTGTCAAGAC CGAGCAGTGC TACAATGTGA ACTGGACAGC GGAGTCCTTC
    CCGAGCAACA AGGAGATGAA GAAGCTGACA TGGCTATTTG GCTGCCCCTT GTTACTGGAC
    GATGTTGCTC AGGAATCCTG GCTTCGTCCT GGCCCCACAG ACCTGCTGCT GGAGGTCGGA
    CCCAGGCTGA ACTTTTCCAC CCCGACATCC AGCAACATTG TGTCAGTGTG CCAGGCTGCA
    GGGCTGGGGG CTGTGGACCG CGTGGAGCCA ACCCGGCGCT ACCTGCTCTC GTTTGCCCAC
    CCACCTTCAG CTGACCTGGA GGCCATTGCC CTGGCTACCC TGCATGACCG GATGACAGAG
    CAGCGCTTCC CCCAGCCCAT CCAGAGCTTC TCTTCAGGGT GCATCCCAGC ACCCCTGAGC
    GGCCCAATTG ACGTGCTGGC TGAGGGCCGG TCTGCCCTGG AGAAAGCCAA CCAGGAGCTA
    GGTCTGGCCC TAGACTCCTG GGACCTGGAT TTCTACACCA AGCGCTTCCA GGAGCTGCAG
    CGCAACCCCA GCACTGTGGA AGTCTTCGAC TTGGCTCAGT CCAATAGCGA ACACAGCCGA
    CACTGGTTCT TCAAGGGTCG ACTCCATGTG GATGGACAGG AGCTGCCACA CTCGCTGTTT
    GAGTCCATCA TGAGCACCCA GGCGTCCTCC AACCCTAACA ACGTCCTCAA GTTCTGTGAC
    AACAGCAGTG CCATCCAGGG GAAAGAAGTC CGATTCTTGC GGCCTAAGGA CCCCACACGG
    CCAAGCTCCT TCCAGCAATG TCAGGGGCTG AGACATGTTG TCTTCACAGC AGAGACACAC
    AACTTCCCTA CAGGAGTAGC CCCCTTCAGT GGTGCAACCA CTGGCACAGG GGGCCGGATC
    CGAGATGTCC AGTGCACAGG CCGAGGGGCC CACGTGGTGG CTGGCACTGC TGGCTATTGC
    TTTGGAAACC TGCACATCCC AGGTTACAGT ATGCCCTGGG AGGATCCAAG CTTCCAGTAT
    CCAGGAAACT TTGCCCGACC CCTGGAGATT GCCATTGAGG CCAGTAATGG GGCTTCTGAC
    TACGGCAACA AGTTTGGGGA ACCAGTGCTT GCTGGCTTTG CCCGCTCCTT GGGCCTTCAG
    CTCCCGGACG GCCAGCGGCG TGAGTGGATC AAGCCCATCA TGTTTAGTGG AGGCATTGGG
    TCCATGGAAG CTGATCATGT GAGCAAGGAG CCCCCAGAGA CAGGCATGGA TGTCGTGAAG
    ATTGGCGGTC CTGTCTACAG GATTGGAGTT GGGGGCGGAG CTGCTTCGTC TGTGCAGGTG
    CAAGGAGACA ACGCCAGTGA CCTGGATTTT GGGGCTGTGC AGCGGGGAGA CCCAGAAATG
    GAGCAGAAGA TGAACCGTGT GATCCGGGCT TGTGTGGAGG CCCCTGGGGG AAACCCCATC
    TGCAGCCTCC ATGACCAGGG TGCTGGTGGC AATGGCAATG TCCTGAAGGA GCTGAGTGAC
    CCGGCTGGAG CCATCATTCA CACCAGCTGC TTCCAGCTGG GGGACCCAAC CCTGAATGCC
    CTGGAAATCT GGGGGGCCGA GTACCAGGAG TCGAACGCAC TTCTGCTGAG GCCCTCTGAC
    CGGGACTTCC TGAGTTGTGT CAGCGCCCGG GAACGCTGCC CAGTCTGCTT TGTGGGCACC
    ATCACTGGAG ACAGGAGAAT AGTGCTGGTG GATGATCGGG AGTGGCCTAT GGGCACAGGT
    GGCCAGGTGG ATGCGCCCCC CACTCCTCCC CCAACCCCTG TGGACCTGGA CCTGGACTGG
    GTGCTGGGCA AGATGCCCCA GAAGGAGTTC TTCCTCCAGA GGAGTCTCCC CTTGCTGCAG
    CCTCTGACTC TGCCCCCAGG GCTGAGCGTG CGCCAGGCTC TGGCGCGGGT CCTCAGGCTG
    CCTGCCGTGG CCAGCAAGCG CTACCTCACC AACAAGGTGG ACCGTTCTGT GGGCGGCCTG
    GTGGCCCAGC AGCAGTGTGT GGGACCCCTG CAGACCCCTC TGGCAGATGT GGCGGTTGTG
    GCACTGAGTC ACCAGGAGCT TGTAGGGGCC GCCACAGCCC TGGGAGAGCA GCCAGTCAAG
    AGCCTGCTGG ACCCGAAGGT TGCCGCCCGG CTGGCTGTGG CCGAAGCCCT CACCAACCTG
    GTATTTGCTC TGGTCACCGA CCTCCAGGAT GTGAAATGCA GCGGGAACTG GATGTGGGCA
    GCCAAGCTCC CAGGGGAGGG TGCAGCTCTG GCTGACGCCT GTGAGGCTAT GGTGGCAGTG
    ATGGCGGCCC TGGGTGTAGC GGTAGACGGT GGCAAGGACT CCCTCAGCAT GGCTGCCCGG
    GTTGGCTCTG AAACCGTGCG GGCTCCTGGG TCACTGGTCA TCTCAGCCTA TGCTGTCTGT
    TCAGACATTA CAGCCACTGT GACCCCAGAC CTCAAGCGTC CTGGGGGGAG AGGCCACCTG
    CTCTACGTGC CTCTGAGTCC TGGGCAGCAC CGGCTGGGGG GCACAGCTCT GGCTCAGTGC
    TTCTCCCAGC TTGGCGAGCA GCCTCCCGAC CTGGATGTTC CTGAGAACTT GGTGCGAGCC
    TTCAGCATCA CCCAGGGGCT GCTGAAAGAC CGCCTCCTCT GCTCAGGCCA TGATGTCAGT
    GATGGAGGTC TCATCACTTG CCTGCTAGAG ATGGCCTTTG CTGGGAATTG TGGGATAGAG
    GTGGACATCC CCGCACCTGG GGTCGATGCC CTGCCTGTGC TGTTCGCCGA GGAGCCAGGC
    CTGGTGCTGG AGGTGCAGGA GCCGGACCTC GCCCAAGTGC TGATGCGTTA CCGGAATGCT
    GGCCTCCACT GCCTAGAGCT GGGTCTCACA GGCGACACGG GGCCCCACGC CATGGTCCGC
    GTGTCGGTGA ACAGGACGGT GGTTCTGGAG GAACCTGTTG GGCAGCTACG AGCCCTCTGG
    GAGGAAACCA GTTTCCAGTT GGACCGGCTA CAGGCAAAAC CACACTGTGT GGCCCAGGAA
    GAGCAAGGGC TGAGGGAGCG GGCAGGGCCC ACCTTCTGCC TGCCGCCTAC CTTCCCCAGA
    GCTTCCGTGC CTTGTGAGCC TGGTGGTCCT GCCCCCCGAG TCGCCATCAT TCGGGAAGAG
    GGCAGTAATG GAGACCGGGA GATGGCAGAT GCCTTCCACT TGGCTGGATT TGAGGTGTGG
    GATGTAACCA TGCAGGACCT GTGCTCTGGA GCCATCGGGC TGGACACATT CCGCGGTGTG
    GCCTTCGTAG GCGGCTTCAG CTACGCGGAC GTCCTGGGCT CTGCCAAAGG CTGGGCAGCT
    GCTGTGACCT TTAACCCGCA GGCCGGGGCT GAGATGAAGC GCTTCCGCCA GCGGCCAGAC
    ACCTTCAGCC TGGGCGTGTG TAACGGCTGT CAGCTGCTGG CCCTGCTGGG CTGGGTGGGA
    GGCAGTTCTA GTGAGGAGGT GGTGGAGATG TGCCAGGAGT CCTGGCCGGC CCGGCCCGGT
    CTCCTGCTAC GCCACAACCT GTCTGGGCGC TTTGAGTCGC GCTGGGCTAG TGTGCGCGTG
    GGGCCCGGGC CAGCCCTGAT GCTGCGAGGG ATGGAAGGCG CGGTGCTGCC CGTGTGGAGC
    GCTCACGGGG AAGGTTACAT GGCATTTTCT TCTCCGGAAC TCCAAGCCCA GATGGAGGCC
    AGGGGCTTAG CTCCACTGCA CTGGGCAGAT GATGATGGGA ACCCCACGGA ACAGTACCCC
    CTGAATCCCA ACGGCTCCCC AGGGGGTGTC GCTGGCATCT GCTCCCCCGA TGGCCGCCAC
    TTGGCCCTCA TGCCTCACCC GGAGCGGTCT GTGAGGCCTT GGCAGTGGGC CTGGCGACCT
    CCTCCATTTG ACACTCTAAC CACCTCCCCC TGGCTCCAGC TCTTCATCAA TGCCCGGAAT
    TGGACCCAGG AATGCAGCTG CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005220416.4
    CDS35..4138
    Translation

    Target ORF information:

    RefSeq Version XM_005220359.4
    Organism Bos taurus(cattle)
    Definition Bos taurus phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005220359.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAGGATGA CGTTGATCTG CGCGCCAGAA TTCGAGGAGC TGACGGACTG GGAGAAGTGC 
    TACCACGCGC AACGGACACC TGCAGAGATG TCCTCCGTCC TTCACTTCTA TGTCCGCCCC
    TCTGGCCACG AGAGGGCAGC CTCTGAATAC ATTCAAAGGA AGCTGCAAAG GGAACTGCCA
    GAGCTGCAGG GTGTCAAGAC CGAGCAGTGC TACAATGTGA ACTGGACAGC GGAGTCCTTC
    CCGAGCAACA AGGAGATGAA GAAGCTGACA TGGCTATTTG GCTGCCCCTT GTTACTGGAC
    GATGTTGCTC AGGAATCCTG GCTTCGTCCT GGCCCCACAG ACCTGCTGCT GGAGGTCGGA
    CCCAGGCTGA ACTTTTCCAC CCCGACATCC AGCAACATTG TGTCAGTGTG CCAGGCTGCA
    GGGCTGGGGG CTGTGGACCG CGTGGAGCCA ACCCGGCGCT ACCTGCTCTC GTTTGCCCAC
    CCACCTTCAG CTGACCTGGA GGCCATTGCC CTGGCTACCC TGCATGACCG GATGACAGAG
    CAGCGCTTCC CCCAGCCCAT CCAGAGCTTC TCTTCAGGGT GCATCCCAGC ACCCCTGAGC
    GGCCCAATTG ACGTGCTGGC TGAGGGCCGG TCTGCCCTGG AGAAAGCCAA CCAGGAGCTA
    GGTCTGGCCC TAGACTCCTG GGACCTGGAT TTCTACACCA AGCGCTTCCA GGAGCTGCAG
    CGCAACCCCA GCACTGTGGA AGTCTTCGAC TTGGCTCAGT CCAATAGCGA ACACAGCCGA
    CACTGGTTCT TCAAGGGTCG ACTCCATGTG GATGGACAGG AGCTGCCACA CTCGCTGTTT
    GAGTCCATCA TGAGCACCCA GGCGTCCTCC AACCCTAACA ACGTCCTCAA GTTCTGTGAC
    AACAGCAGTG CCATCCAGGG GAAAGAAGTC CGATTCTTGC GGCCTAAGGA CCCCACACGG
    CCAAGCTCCT TCCAGCAATG TCAGGGGCTG AGACATGTTG TCTTCACAGC AGAGACACAC
    AACTTCCCTA CAGGAGTAGC CCCCTTCAGT GGTGCAACCA CTGGCACAGG GGGCCGGATC
    CGAGATGTCC AGTGCACAGG CCGAGGGGCC CACGTGGTGG CTGGCACTGC TGGCTATTGC
    TTTGGAAACC TGCACATCCC AGGTTACAGT ATGCCCTGGG AGGATCCAAG CTTCCAGTAT
    CCAGGAAACT TTGCCCGACC CCTGGAGATT GCCATTGAGG CCAGTAATGG GGCTTCTGAC
    TACGGCAACA AGTTTGGGGA ACCAGTGCTT GCTGGCTTTG CCCGCTCCTT GGGCCTTCAG
    CTCCCGGACG GCCAGCGGCG TGAGTGGATC AAGCCCATCA TGTTTAGTGG AGGCATTGGG
    TCCATGGAAG CTGATCATGT GAGCAAGGAG CCCCCAGAGA CAGGCATGGA TGTCGTGAAG
    ATTGGCGGTC CTGTCTACAG GATTGGAGTT GGGGGCGGAG CTGCTTCGTC TGTGCAGGTG
    CAAGGAGACA ACGCCAGTGA CCTGGATTTT GGGGCTGTGC AGCGGGGAGA CCCAGAAATG
    GAGCAGAAGA TGAACCGTGT GATCCGGGCT TGTGTGGAGG CCCCTGGGGG AAACCCCATC
    TGCAGCCTCC ATGACCAGGG TGCTGGTGGC AATGGCAATG TCCTGAAGGA GCTGAGTGAC
    CCGGCTGGAG CCATCATTCA CACCAGCTGC TTCCAGCTGG GGGACCCAAC CCTGAATGCC
    CTGGAAATCT GGGGGGCCGA GTACCAGGAG TCGAACGCAC TTCTGCTGAG GCCCTCTGAC
    CGGGACTTCC TGAGTTGTGT CAGCGCCCGG GAACGCTGCC CAGTCTGCTT TGTGGGCACC
    ATCACTGGAG ACAGGAGAAT AGTGCTGGTG GATGATCGGG AGTGGCCTAT GGGCACAGGT
    GGCCAGGTGG ATGCGCCCCC CACTCCTCCC CCAACCCCTG TGGACCTGGA CCTGGACTGG
    GTGCTGGGCA AGATGCCCCA GAAGGAGTTC TTCCTCCAGA GGAGTCTCCC CTTGCTGCAG
    CCTCTGACTC TGCCCCCAGG GCTGAGCGTG CGCCAGGCTC TGGCGCGGGT CCTCAGGCTG
    CCTGCCGTGG CCAGCAAGCG CTACCTCACC AACAAGGTGG ACCGTTCTGT GGGCGGCCTG
    GTGGCCCAGC AGCAGTGTGT GGGACCCCTG CAGACCCCTC TGGCAGATGT GGCGGTTGTG
    GCACTGAGTC ACCAGGAGCT TGTAGGGGCC GCCACAGCCC TGGGAGAGCA GCCAGTCAAG
    AGCCTGCTGG ACCCGAAGGT TGCCGCCCGG CTGGCTGTGG CCGAAGCCCT CACCAACCTG
    GTATTTGCTC TGGTCACCGA CCTCCAGGAT GTGAAATGCA GCGGGAACTG GATGTGGGCA
    GCCAAGCTCC CAGGGGAGGG TGCAGCTCTG GCTGACGCCT GTGAGGCTAT GGTGGCAGTG
    ATGGCGGCCC TGGGTGTAGC GGTAGACGGT GGCAAGGACT CCCTCAGCAT GGCTGCCCGG
    GTTGGCTCTG AAACCGTGCG GGCTCCTGGG TCACTGGTCA TCTCAGCCTA TGCTGTCTGT
    TCAGACATTA CAGCCACTGT GACCCCAGAC CTCAAGCGTC CTGGGGGGAG AGGCCACCTG
    CTCTACGTGC CTCTGAGTCC TGGGCAGCAC CGGCTGGGGG GCACAGCTCT GGCTCAGTGC
    TTCTCCCAGC TTGGCGAGCA GCCTCCCGAC CTGGATGTTC CTGAGAACTT GGTGCGAGCC
    TTCAGCATCA CCCAGGGGCT GCTGAAAGAC CGCCTCCTCT GCTCAGGCCA TGATGTCAGT
    GATGGAGGTC TCATCACTTG CCTGCTAGAG ATGGCCTTTG CTGGGAATTG TGGGATAGAG
    GTGGACATCC CCGCACCTGG GGTCGATGCC CTGCCTGTGC TGTTCGCCGA GGAGCCAGGC
    CTGGTGCTGG AGGTGCAGGA GCCGGACCTC GCCCAAGTGC TGATGCGTTA CCGGAATGCT
    GGCCTCCACT GCCTAGAGCT GGGTCTCACA GGCGACACGG GGCCCCACGC CATGGTCCGC
    GTGTCGGTGA ACAGGACGGT GGTTCTGGAG GAACCTGTTG GGCAGCTACG AGCCCTCTGG
    GAGGAAACCA GTTTCCAGTT GGACCGGCTA CAGGCAAAAC CACACTGTGT GGCCCAGGAA
    GAGCAAGGGC TGAGGGAGCG GGCAGGGCCC ACCTTCTGCC TGCCGCCTAC CTTCCCCAGA
    GCTTCCGTGC CTTGTGAGCC TGGTGGTCCT GCCCCCCGAG TCGCCATCAT TCGGGAAGAG
    GGCAGTAATG GAGACCGGGA GATGGCAGAT GCCTTCCACT TGGCTGGATT TGAGGTGTGG
    GATGTAACCA TGCAGGACCT GTGCTCTGGA GCCATCGGGC TGGACACATT CCGCGGTGTG
    GCCTTCGTAG GCGGCTTCAG CTACGCGGAC GTCCTGGGCT CTGCCAAAGG CTGGGCAGCT
    GCTGTGACCT TTAACCCGCA GGCCGGGGCT GAGATGAAGC GCTTCCGCCA GCGGCCAGAC
    ACCTTCAGCC TGGGCGTGTG TAACGGCTGT CAGCTGCTGG CCCTGCTGGG CTGGGTGGGA
    GGCAGTTCTA GTGAGGAGGT GGTGGAGATG TGCCAGGAGT CCTGGCCGGC CCGGCCCGGT
    CTCCTGCTAC GCCACAACCT GTCTGGGCGC TTTGAGTCGC GCTGGGCTAG TGTGCGCGTG
    GGGCCCGGGC CAGCCCTGAT GCTGCGAGGG ATGGAAGGCG CGGTGCTGCC CGTGTGGAGC
    GCTCACGGGG AAGGTTACAT GGCATTTTCT TCTCCGGAAC TCCAAGCCCA GATGGAGGCC
    AGGGGCTTAG CTCCACTGCA CTGGGCAGAT GATGATGGGA ACCCCACGGA ACAGTACCCC
    CTGAATCCCA ACGGCTCCCC AGGGGGTGTC GCTGGCATCT GCTCCCCCGA TGGCCGCCAC
    TTGGCCCTCA TGCCTCACCC GGAGCGGTCT GTGAGGCCTT GGCAGTGGGC CTGGCGACCT
    CCTCCATTTG ACACTCTAAC CACCTCCCCC TGGCTCCAGC TCTTCATCAA TGCCCGGAAT
    TGGACCCAGG AATGCAGCTG CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93869
    Clone ID Related Accession (Same CDS sequence) XM_015458726.1
    Accession Version XM_015458726.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4008bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product phosphoribosylformylglycinamidine synthase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104494.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTCCTCCG TCCTTCACTT CTATGTCCGC CCCTCTGGCC ACGAGAGGGC AGCCTCTGAA 
    TACATTCAAA GGAAGCTGCA AAGGGAACTG CCAGAGCTGC AGGGTGTCAA GACCGAGCAG
    TGCTACAATG TGAACTGGGC AGCGGAGTCC TTCCCGAGCA ACAAGGAGAT GAAGAAGCTG
    ACATGGCTAT TTGGCTGCCC CTTGTTACTG GACGATGTTG CTCAGGAATC CTGGCTTCGT
    CCTGGCCCCA CAGACCTGCT GCTGGAGGTC GGACCCAGGC TGAACTTTTC CACCCCGACA
    TCCAGCAACA TTGTGTCAGT GTGCCAGGCT GCAGGGCTGG GGGCTGTGGA CCGCGTGGAG
    CCAACCCGGC GCTACCTGCT CTCGTTTGCC CACCCACCTT CAGCTGACCT GGAGGCCATT
    GCCCTGGCTA CCCTGCATGA CCGGATGACA GAGCAGCACT TCCCCCAGCC CATCCAGAGC
    TTCTCTTCAG GGTGCATCCC AGCACCCCTG AGCGGCCCAA TTGACGTGCT GGCTGAGGGC
    CGGTCTGCCC TGGAGAAAGC CAACCAGGAG CTAGGTCTGG CCCTAGACTC CTGGGACCTG
    GATTTCTACA CCAAGCGCTT CCAGGAGCTG CAGCGCAACC CCAGCACTGT GGAAGTCTTC
    GACTTGGCTC AGTCCAATAG CGAACACAGC CGACACTGGT TCTTCAAGGG TCGACTCCAT
    GTGGATGGAC AGGAGCTGCC ACACTCGCTG TTTGAGTCCA TCATGAGCAC CCAGGCGTCC
    TCCAACCCTA ACAACGTCCT CAAGTTCTGT GACAACAGCA GTGCCATCCA GGGGAAAGAA
    GTCCGATTCT TGCGGCCTAA GGACCCCACA CGGCCAAGCT CCTTCCAGCA ATGTCAGGGG
    CTGAGACATG TTGTCTTCAC AGCAGAGACA CACAACTTCC CTACAGGAGT AGCCCCCTTC
    AGTGGTGCAG CCACTGGCAC AGGGGGCCGG ATCCGAGATG TCCAGTGCAC AGGCCGAGGG
    GCCCACGTGG TGGCTGGCAC TGCTGGCTAT TGCTTTGGAA ACCTGCACAT CCCAGGTTAC
    AGTATGCCCT GGGAGGATCC AAGCTTCCAG TATCCAGGAA ACTTTGCCCG ACCCCTGGAG
    ATTGCCATTG AGGCCAGTAA TGGGGCTTCT GACTACGGCA ACAAGTTTGG GGAACCAGTG
    CTTGCTGGCT TTGCCCGCTC CTTGGGCCTT CAGCTTCCGG ACGGCCAGCG GCGTGAGTGG
    ATCAAGCCCA TAATGTTTAG TGGAGGCATT GGGTCCATGG AAGCTGAGCA TGTGAGCAAG
    GAGCCCCCAG AGACAGGCAT GGATGTCGTG AAGATTGGCG GTCCTGTCTA CAGGATTGGA
    GTTGGGGGCG GAGCTGCTTC GTCTGTGCAG GTGCAAGGAG ACAACGCCAG TGACCTGGAT
    TTTGGGGCTG TGCAGCGGGG AGACCCAGAG ATGGAGCAGA AGATGAACCG TGTGATCCGG
    GCTTGTGTGG AGGCCCCTGG GGGAAACCCC ATCTGCAGCC TCCATGACCA GGGTGCTGGT
    GGCAATGGCA ATGTCCTGAA GGAGCTGAGT GACCCGGCTG GAGCCGTCAT TCACACCAGC
    TGCTTCCAGC TGGGGGACCC AACCCTGAAT GCCCTGGAAA TCTGGGGGGC CGAGTACCAG
    GAGTCGAACG CACTTCTGCT GAGGCCCTCT GACCGGGACT TCCTGAGTTG TGTCAGCGCC
    CGGGAACGCT GCCCAGTCTG CTTTGTGGGC ACCATCACTG GAGACAGGAG AATAGTGCTG
    GTGGATGATC GGGAGTGGCC TATGGGCACA GGTGGCCAGG TGGATGCGCC CCCCACTCCT
    CCCCCAACCC CTGTGGACCT GGACCTGGAC TGGGTGCTGG GCAAGATGCC CCAGAAGGAG
    TTCTTCCTCC AGAGGAGTCT CCCCTTGCTG CAGCCTCTGA CTCTGCCCCC AGGGCTGAGC
    GTGCGCCAGG CTCTGGCGCG GGTCCTCAGG CTGCCTGCCG TGGCCAGCAA GCGCTACCTC
    ACCAACAAGG TGGACCGTTC TGTGGGCGGC CTGGTGGCCC AGCAGCAGTG TGTGGGACCC
    CTGCAGACCC CTCTGGCAGA TGTGGCGGTT GTGGCACTGA GTCACCAGGA GCTTGTAGGG
    GCCGCCACAG CCCTGGGAGA GCAGCCAGTC AAGAGCCTGC TGGACCCGAA GGTTGCCGCC
    CGGCTGGCTG TGGCCGAAGC CCTCACCAAC CTGGTATTTG CTCTGGTCAC CGACCTCCAG
    GATGTGAAAT GCAGCGGGAA CTGGATGTGG GCAGCCAAGC TCCCAGGGGA GGGTGCAGCT
    CTGGCTGACG CCTGTGAGGC TATGGTGGCA GTGATGGCGG CCCTGGGTGT AGCGGTAGAC
    GGTGGCAAGG ACTCCCTCAG CATGGCTGCC CGGGTTGGCT CTGAAACCGT GCGGGCTCCT
    GGGTCACTGG TCATCTCAGC CTATGCTGTC TGTTCAGACA TTACAGCCAC TGTGACCCCA
    GACCTCAAGC GTCCTGGGGG GAGAGGCCAC CTGCTCTACG TGCCTCTGAG TCCTGGGCAG
    CACCGGCTGG GGGGCACAGC TCTGGCTCAG TGCTTCTCCC AGCTTGGCGA GCAGCCTCCC
    GACCTGGATG TTCCTGAGAA CTTGGTGCGA GCCTTCAGCA TCACCCAGGG GCTGCTGAAA
    GACCGCCTCC TCTGCTCAGG CCATGATGTC AGTGATGGAG GTCTCATCAC TTGCCTGCTA
    GAGATGGCCT TTGCTGGGAA TTGTGGGATA GAGGTGGACA TCCCCGCACC TGGGGTCGAT
    GCCCTGCCTG TGCTGTTCGC CGAGGAGCCA GGCCTGGTGC TGGAGGTGCA GGAGCCGGAC
    CTCGCCCAAG TGCTGATGCG TTACCGGAAT GCTGGCCTCC ACTGCCTAGA GCTGGGTCTC
    ACAGGCGACA CGGGGCCCCA CGCCATGGTC CGCGTGTCGG TGAACAGGAC GGTGGTTCTG
    GAGGAACCTG TTGGGCAGCT ACGAGCCCTC TGGGAGGAAA CCAGTTTCCA GTTGGACCGG
    CTACAGGCAA AACCACACTG TGTGGCCCAG GAAGAGCAAG GGCTGAGGGA GCGGGCAGGG
    CCCACCTTCT GCCTGCCGCC TACCTTCCCC AGAGCTTCCG TGCCTTGTGG TCCTGCCCCC
    CGAGTCGCCA TCATTCGGGA AGAGGGCAGT AATGGAGACC GGGAGATGGC AGATGCCTTC
    CACTTGGCTG GATTTGAGGT GTGGGATGTA ACCATGCAGG ACCTGTGCTC TGGAGCCATC
    GGGCTGGACA CATTCCGCGG TGTGGCCTTC GTAGGCGGCT TCAGCTACGC GGACGTCCTG
    GGCTCTGCCA AAGGCTGGGC AGCTGCTGTG ACCTTTAACC CGCAGGCCGG GGCTGAGATG
    AAGCGCTTCC GCCAGCGGCC AGACACCTTC AGCCTGGGCG TGTGTAACGG CTGTCAGCTG
    CTGGCCCTGC TGGGCTGGGT GGGAGGCAGT TCTAGTGAGG AGGTGGTGGA GATGTGCCAG
    GAGTCCTGGC CGGCCCGGCC CGGTCTCCTG CTACGCCACA ACCTGTCTGG GCGCTTTGAG
    TCGCGCTGGG CTAGTGTGCG CGTGGGGCCC GGGCCAGCCC TGATGCTGCG AGGGATGGAA
    GGCGCGGTGC TGCCCGTGTG GAGCGCTCAC GGGGAAGGTT ACATGGCATT TTCTTCTCCG
    GAACTCCAAG CCCAGATGGA GGCCAGGGGC TTAGCTCCAC TGCACTGGGC AGATGATGAT
    GGGAACCCCA CGGAACAGTA CCCCCTGAAT CCCAACGGCT CCCCAGGGGG TGTCGCTGGC
    ATCTGCTCCC CCGATGGCCG CCACTTGGCC CTCATGCCTC ACCCGGAGCG GTCTGTGAGG
    CCTTGGCAGT GGGCCTGGCG ACCTCCTCCA TTTGACACTC TAACCACCTC CCCCTGGCTC
    CAGCTCTTCA TCAATGCCCG GAATTGGACC CAGGAATGCA GCTGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015314212.1
    CDS40..4047
    Translation

    Target ORF information:

    RefSeq Version XM_015458726.1
    Organism Bos taurus(cattle)
    Definition Bos taurus phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015458726.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTCCTCCG TCCTTCACTT CTATGTCCGC CCCTCTGGCC ACGAGAGGGC AGCCTCTGAA 
    TACATTCAAA GGAAGCTGCA AAGGGAACTG CCAGAGCTGC AGGGTGTCAA GACCGAGCAG
    TGCTACAATG TGAACTGGGC AGCGGAGTCC TTCCCGAGCA ACAAGGAGAT GAAGAAGCTG
    ACATGGCTAT TTGGCTGCCC CTTGTTACTG GACGATGTTG CTCAGGAATC CTGGCTTCGT
    CCTGGCCCCA CAGACCTGCT GCTGGAGGTC GGACCCAGGC TGAACTTTTC CACCCCGACA
    TCCAGCAACA TTGTGTCAGT GTGCCAGGCT GCAGGGCTGG GGGCTGTGGA CCGCGTGGAG
    CCAACCCGGC GCTACCTGCT CTCGTTTGCC CACCCACCTT CAGCTGACCT GGAGGCCATT
    GCCCTGGCTA CCCTGCATGA CCGGATGACA GAGCAGCACT TCCCCCAGCC CATCCAGAGC
    TTCTCTTCAG GGTGCATCCC AGCACCCCTG AGCGGCCCAA TTGACGTGCT GGCTGAGGGC
    CGGTCTGCCC TGGAGAAAGC CAACCAGGAG CTAGGTCTGG CCCTAGACTC CTGGGACCTG
    GATTTCTACA CCAAGCGCTT CCAGGAGCTG CAGCGCAACC CCAGCACTGT GGAAGTCTTC
    GACTTGGCTC AGTCCAATAG CGAACACAGC CGACACTGGT TCTTCAAGGG TCGACTCCAT
    GTGGATGGAC AGGAGCTGCC ACACTCGCTG TTTGAGTCCA TCATGAGCAC CCAGGCGTCC
    TCCAACCCTA ACAACGTCCT CAAGTTCTGT GACAACAGCA GTGCCATCCA GGGGAAAGAA
    GTCCGATTCT TGCGGCCTAA GGACCCCACA CGGCCAAGCT CCTTCCAGCA ATGTCAGGGG
    CTGAGACATG TTGTCTTCAC AGCAGAGACA CACAACTTCC CTACAGGAGT AGCCCCCTTC
    AGTGGTGCAG CCACTGGCAC AGGGGGCCGG ATCCGAGATG TCCAGTGCAC AGGCCGAGGG
    GCCCACGTGG TGGCTGGCAC TGCTGGCTAT TGCTTTGGAA ACCTGCACAT CCCAGGTTAC
    AGTATGCCCT GGGAGGATCC AAGCTTCCAG TATCCAGGAA ACTTTGCCCG ACCCCTGGAG
    ATTGCCATTG AGGCCAGTAA TGGGGCTTCT GACTACGGCA ACAAGTTTGG GGAACCAGTG
    CTTGCTGGCT TTGCCCGCTC CTTGGGCCTT CAGCTTCCGG ACGGCCAGCG GCGTGAGTGG
    ATCAAGCCCA TAATGTTTAG TGGAGGCATT GGGTCCATGG AAGCTGAGCA TGTGAGCAAG
    GAGCCCCCAG AGACAGGCAT GGATGTCGTG AAGATTGGCG GTCCTGTCTA CAGGATTGGA
    GTTGGGGGCG GAGCTGCTTC GTCTGTGCAG GTGCAAGGAG ACAACGCCAG TGACCTGGAT
    TTTGGGGCTG TGCAGCGGGG AGACCCAGAG ATGGAGCAGA AGATGAACCG TGTGATCCGG
    GCTTGTGTGG AGGCCCCTGG GGGAAACCCC ATCTGCAGCC TCCATGACCA GGGTGCTGGT
    GGCAATGGCA ATGTCCTGAA GGAGCTGAGT GACCCGGCTG GAGCCGTCAT TCACACCAGC
    TGCTTCCAGC TGGGGGACCC AACCCTGAAT GCCCTGGAAA TCTGGGGGGC CGAGTACCAG
    GAGTCGAACG CACTTCTGCT GAGGCCCTCT GACCGGGACT TCCTGAGTTG TGTCAGCGCC
    CGGGAACGCT GCCCAGTCTG CTTTGTGGGC ACCATCACTG GAGACAGGAG AATAGTGCTG
    GTGGATGATC GGGAGTGGCC TATGGGCACA GGTGGCCAGG TGGATGCGCC CCCCACTCCT
    CCCCCAACCC CTGTGGACCT GGACCTGGAC TGGGTGCTGG GCAAGATGCC CCAGAAGGAG
    TTCTTCCTCC AGAGGAGTCT CCCCTTGCTG CAGCCTCTGA CTCTGCCCCC AGGGCTGAGC
    GTGCGCCAGG CTCTGGCGCG GGTCCTCAGG CTGCCTGCCG TGGCCAGCAA GCGCTACCTC
    ACCAACAAGG TGGACCGTTC TGTGGGCGGC CTGGTGGCCC AGCAGCAGTG TGTGGGACCC
    CTGCAGACCC CTCTGGCAGA TGTGGCGGTT GTGGCACTGA GTCACCAGGA GCTTGTAGGG
    GCCGCCACAG CCCTGGGAGA GCAGCCAGTC AAGAGCCTGC TGGACCCGAA GGTTGCCGCC
    CGGCTGGCTG TGGCCGAAGC CCTCACCAAC CTGGTATTTG CTCTGGTCAC CGACCTCCAG
    GATGTGAAAT GCAGCGGGAA CTGGATGTGG GCAGCCAAGC TCCCAGGGGA GGGTGCAGCT
    CTGGCTGACG CCTGTGAGGC TATGGTGGCA GTGATGGCGG CCCTGGGTGT AGCGGTAGAC
    GGTGGCAAGG ACTCCCTCAG CATGGCTGCC CGGGTTGGCT CTGAAACCGT GCGGGCTCCT
    GGGTCACTGG TCATCTCAGC CTATGCTGTC TGTTCAGACA TTACAGCCAC TGTGACCCCA
    GACCTCAAGC GTCCTGGGGG GAGAGGCCAC CTGCTCTACG TGCCTCTGAG TCCTGGGCAG
    CACCGGCTGG GGGGCACAGC TCTGGCTCAG TGCTTCTCCC AGCTTGGCGA GCAGCCTCCC
    GACCTGGATG TTCCTGAGAA CTTGGTGCGA GCCTTCAGCA TCACCCAGGG GCTGCTGAAA
    GACCGCCTCC TCTGCTCAGG CCATGATGTC AGTGATGGAG GTCTCATCAC TTGCCTGCTA
    GAGATGGCCT TTGCTGGGAA TTGTGGGATA GAGGTGGACA TCCCCGCACC TGGGGTCGAT
    GCCCTGCCTG TGCTGTTCGC CGAGGAGCCA GGCCTGGTGC TGGAGGTGCA GGAGCCGGAC
    CTCGCCCAAG TGCTGATGCG TTACCGGAAT GCTGGCCTCC ACTGCCTAGA GCTGGGTCTC
    ACAGGCGACA CGGGGCCCCA CGCCATGGTC CGCGTGTCGG TGAACAGGAC GGTGGTTCTG
    GAGGAACCTG TTGGGCAGCT ACGAGCCCTC TGGGAGGAAA CCAGTTTCCA GTTGGACCGG
    CTACAGGCAA AACCACACTG TGTGGCCCAG GAAGAGCAAG GGCTGAGGGA GCGGGCAGGG
    CCCACCTTCT GCCTGCCGCC TACCTTCCCC AGAGCTTCCG TGCCTTGTGG TCCTGCCCCC
    CGAGTCGCCA TCATTCGGGA AGAGGGCAGT AATGGAGACC GGGAGATGGC AGATGCCTTC
    CACTTGGCTG GATTTGAGGT GTGGGATGTA ACCATGCAGG ACCTGTGCTC TGGAGCCATC
    GGGCTGGACA CATTCCGCGG TGTGGCCTTC GTAGGCGGCT TCAGCTACGC GGACGTCCTG
    GGCTCTGCCA AAGGCTGGGC AGCTGCTGTG ACCTTTAACC CGCAGGCCGG GGCTGAGATG
    AAGCGCTTCC GCCAGCGGCC AGACACCTTC AGCCTGGGCG TGTGTAACGG CTGTCAGCTG
    CTGGCCCTGC TGGGCTGGGT GGGAGGCAGT TCTAGTGAGG AGGTGGTGGA GATGTGCCAG
    GAGTCCTGGC CGGCCCGGCC CGGTCTCCTG CTACGCCACA ACCTGTCTGG GCGCTTTGAG
    TCGCGCTGGG CTAGTGTGCG CGTGGGGCCC GGGCCAGCCC TGATGCTGCG AGGGATGGAA
    GGCGCGGTGC TGCCCGTGTG GAGCGCTCAC GGGGAAGGTT ACATGGCATT TTCTTCTCCG
    GAACTCCAAG CCCAGATGGA GGCCAGGGGC TTAGCTCCAC TGCACTGGGC AGATGATGAT
    GGGAACCCCA CGGAACAGTA CCCCCTGAAT CCCAACGGCT CCCCAGGGGG TGTCGCTGGC
    ATCTGCTCCC CCGATGGCCG CCACTTGGCC CTCATGCCTC ACCCGGAGCG GTCTGTGAGG
    CCTTGGCAGT GGGCCTGGCG ACCTCCTCCA TTTGACACTC TAACCACCTC CCCCTGGCTC
    CAGCTCTTCA TCAATGCCCG GAATTGGACC CAGGAATGCA GCTGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93870
    Clone ID Related Accession (Same CDS sequence) XM_005220359.3
    Accession Version XM_005220359.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4104bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product phosphoribosylformylglycinamidine synthase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104494.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005220359.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAGGATGA CGTTGATCTG CGCGCCAGAA TTCGAGGAGC TGACGGACTG GGAGAAGTGC 
    TACCACGCGC AACGGACACC TGCAGAGATG TCCTCCGTCC TTCACTTCTA TGTCCGCCCC
    TCTGGCCACG AGAGGGCAGC CTCTGAATAC ATTCAAAGGA AGCTGCAAAG GGAACTGCCA
    GAGCTGCAGG GTGTCAAGAC CGAGCAGTGC TACAATGTGA ACTGGGCAGC GGAGTCCTTC
    CCGAGCAACA AGGAGATGAA GAAGCTGACA TGGCTATTTG GCTGCCCCTT GTTACTGGAC
    GATGTTGCTC AGGAATCCTG GCTTCGTCCT GGCCCCACAG ACCTGCTGCT GGAGGTCGGA
    CCCAGGCTGA ACTTTTCCAC CCCGACATCC AGCAACATTG TGTCAGTGTG CCAGGCTGCA
    GGGCTGGGGG CTGTGGACCG CGTGGAGCCA ACCCGGCGCT ACCTGCTCTC GTTTGCCCAC
    CCACCTTCAG CTGACCTGGA GGCCATTGCC CTGGCTACCC TGCATGACCG GATGACAGAG
    CAGCACTTCC CCCAGCCCAT CCAGAGCTTC TCTTCAGGGT GCATCCCAGC ACCCCTGAGC
    GGCCCAATTG ACGTGCTGGC TGAGGGCCGG TCTGCCCTGG AGAAAGCCAA CCAGGAGCTA
    GGTCTGGCCC TAGACTCCTG GGACCTGGAT TTCTACACCA AGCGCTTCCA GGAGCTGCAG
    CGCAACCCCA GCACTGTGGA AGTCTTCGAC TTGGCTCAGT CCAATAGCGA ACACAGCCGA
    CACTGGTTCT TCAAGGGTCG ACTCCATGTG GATGGACAGG AGCTGCCACA CTCGCTGTTT
    GAGTCCATCA TGAGCACCCA GGCGTCCTCC AACCCTAACA ACGTCCTCAA GTTCTGTGAC
    AACAGCAGTG CCATCCAGGG GAAAGAAGTC CGATTCTTGC GGCCTAAGGA CCCCACACGG
    CCAAGCTCCT TCCAGCAATG TCAGGGGCTG AGACATGTTG TCTTCACAGC AGAGACACAC
    AACTTCCCTA CAGGAGTAGC CCCCTTCAGT GGTGCAGCCA CTGGCACAGG GGGCCGGATC
    CGAGATGTCC AGTGCACAGG CCGAGGGGCC CACGTGGTGG CTGGCACTGC TGGCTATTGC
    TTTGGAAACC TGCACATCCC AGGTTACAGT ATGCCCTGGG AGGATCCAAG CTTCCAGTAT
    CCAGGAAACT TTGCCCGACC CCTGGAGATT GCCATTGAGG CCAGTAATGG GGCTTCTGAC
    TACGGCAACA AGTTTGGGGA ACCAGTGCTT GCTGGCTTTG CCCGCTCCTT GGGCCTTCAG
    CTTCCGGACG GCCAGCGGCG TGAGTGGATC AAGCCCATAA TGTTTAGTGG AGGCATTGGG
    TCCATGGAAG CTGAGCATGT GAGCAAGGAG CCCCCAGAGA CAGGCATGGA TGTCGTGAAG
    ATTGGCGGTC CTGTCTACAG GATTGGAGTT GGGGGCGGAG CTGCTTCGTC TGTGCAGGTG
    CAAGGAGACA ACGCCAGTGA CCTGGATTTT GGGGCTGTGC AGCGGGGAGA CCCAGAGATG
    GAGCAGAAGA TGAACCGTGT GATCCGGGCT TGTGTGGAGG CCCCTGGGGG AAACCCCATC
    TGCAGCCTCC ATGACCAGGG TGCTGGTGGC AATGGCAATG TCCTGAAGGA GCTGAGTGAC
    CCGGCTGGAG CCGTCATTCA CACCAGCTGC TTCCAGCTGG GGGACCCAAC CCTGAATGCC
    CTGGAAATCT GGGGGGCCGA GTACCAGGAG TCGAACGCAC TTCTGCTGAG GCCCTCTGAC
    CGGGACTTCC TGAGTTGTGT CAGCGCCCGG GAACGCTGCC CAGTCTGCTT TGTGGGCACC
    ATCACTGGAG ACAGGAGAAT AGTGCTGGTG GATGATCGGG AGTGGCCTAT GGGCACAGGT
    GGCCAGGTGG ATGCGCCCCC CACTCCTCCC CCAACCCCTG TGGACCTGGA CCTGGACTGG
    GTGCTGGGCA AGATGCCCCA GAAGGAGTTC TTCCTCCAGA GGAGTCTCCC CTTGCTGCAG
    CCTCTGACTC TGCCCCCAGG GCTGAGCGTG CGCCAGGCTC TGGCGCGGGT CCTCAGGCTG
    CCTGCCGTGG CCAGCAAGCG CTACCTCACC AACAAGGTGG ACCGTTCTGT GGGCGGCCTG
    GTGGCCCAGC AGCAGTGTGT GGGACCCCTG CAGACCCCTC TGGCAGATGT GGCGGTTGTG
    GCACTGAGTC ACCAGGAGCT TGTAGGGGCC GCCACAGCCC TGGGAGAGCA GCCAGTCAAG
    AGCCTGCTGG ACCCGAAGGT TGCCGCCCGG CTGGCTGTGG CCGAAGCCCT CACCAACCTG
    GTATTTGCTC TGGTCACCGA CCTCCAGGAT GTGAAATGCA GCGGGAACTG GATGTGGGCA
    GCCAAGCTCC CAGGGGAGGG TGCAGCTCTG GCTGACGCCT GTGAGGCTAT GGTGGCAGTG
    ATGGCGGCCC TGGGTGTAGC GGTAGACGGT GGCAAGGACT CCCTCAGCAT GGCTGCCCGG
    GTTGGCTCTG AAACCGTGCG GGCTCCTGGG TCACTGGTCA TCTCAGCCTA TGCTGTCTGT
    TCAGACATTA CAGCCACTGT GACCCCAGAC CTCAAGCGTC CTGGGGGGAG AGGCCACCTG
    CTCTACGTGC CTCTGAGTCC TGGGCAGCAC CGGCTGGGGG GCACAGCTCT GGCTCAGTGC
    TTCTCCCAGC TTGGCGAGCA GCCTCCCGAC CTGGATGTTC CTGAGAACTT GGTGCGAGCC
    TTCAGCATCA CCCAGGGGCT GCTGAAAGAC CGCCTCCTCT GCTCAGGCCA TGATGTCAGT
    GATGGAGGTC TCATCACTTG CCTGCTAGAG ATGGCCTTTG CTGGGAATTG TGGGATAGAG
    GTGGACATCC CCGCACCTGG GGTCGATGCC CTGCCTGTGC TGTTCGCCGA GGAGCCAGGC
    CTGGTGCTGG AGGTGCAGGA GCCGGACCTC GCCCAAGTGC TGATGCGTTA CCGGAATGCT
    GGCCTCCACT GCCTAGAGCT GGGTCTCACA GGCGACACGG GGCCCCACGC CATGGTCCGC
    GTGTCGGTGA ACAGGACGGT GGTTCTGGAG GAACCTGTTG GGCAGCTACG AGCCCTCTGG
    GAGGAAACCA GTTTCCAGTT GGACCGGCTA CAGGCAAAAC CACACTGTGT GGCCCAGGAA
    GAGCAAGGGC TGAGGGAGCG GGCAGGGCCC ACCTTCTGCC TGCCGCCTAC CTTCCCCAGA
    GCTTCCGTGC CTTGTGAGCC TGGTGGTCCT GCCCCCCGAG TCGCCATCAT TCGGGAAGAG
    GGCAGTAATG GAGACCGGGA GATGGCAGAT GCCTTCCACT TGGCTGGATT TGAGGTGTGG
    GATGTAACCA TGCAGGACCT GTGCTCTGGA GCCATCGGGC TGGACACATT CCGCGGTGTG
    GCCTTCGTAG GCGGCTTCAG CTACGCGGAC GTCCTGGGCT CTGCCAAAGG CTGGGCAGCT
    GCTGTGACCT TTAACCCGCA GGCCGGGGCT GAGATGAAGC GCTTCCGCCA GCGGCCAGAC
    ACCTTCAGCC TGGGCGTGTG TAACGGCTGT CAGCTGCTGG CCCTGCTGGG CTGGGTGGGA
    GGCAGTTCTA GTGAGGAGGT GGTGGAGATG TGCCAGGAGT CCTGGCCGGC CCGGCCCGGT
    CTCCTGCTAC GCCACAACCT GTCTGGGCGC TTTGAGTCGC GCTGGGCTAG TGTGCGCGTG
    GGGCCCGGGC CAGCCCTGAT GCTGCGAGGG ATGGAAGGCG CGGTGCTGCC CGTGTGGAGC
    GCTCACGGGG AAGGTTACAT GGCATTTTCT TCTCCGGAAC TCCAAGCCCA GATGGAGGCC
    AGGGGCTTAG CTCCACTGCA CTGGGCAGAT GATGATGGGA ACCCCACGGA ACAGTACCCC
    CTGAATCCCA ACGGCTCCCC AGGGGGTGTC GCTGGCATCT GCTCCCCCGA TGGCCGCCAC
    TTGGCCCTCA TGCCTCACCC GGAGCGGTCT GTGAGGCCTT GGCAGTGGGC CTGGCGACCT
    CCTCCATTTG ACACTCTAAC CACCTCCCCC TGGCTCCAGC TCTTCATCAA TGCCCGGAAT
    TGGACCCAGG AATGCAGCTG CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005220416.3
    CDS19..4122
    Translation

    Target ORF information:

    RefSeq Version XM_005220359.3
    Organism Bos taurus(cattle)
    Definition Bos taurus phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005220359.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAGGATGA CGTTGATCTG CGCGCCAGAA TTCGAGGAGC TGACGGACTG GGAGAAGTGC 
    TACCACGCGC AACGGACACC TGCAGAGATG TCCTCCGTCC TTCACTTCTA TGTCCGCCCC
    TCTGGCCACG AGAGGGCAGC CTCTGAATAC ATTCAAAGGA AGCTGCAAAG GGAACTGCCA
    GAGCTGCAGG GTGTCAAGAC CGAGCAGTGC TACAATGTGA ACTGGGCAGC GGAGTCCTTC
    CCGAGCAACA AGGAGATGAA GAAGCTGACA TGGCTATTTG GCTGCCCCTT GTTACTGGAC
    GATGTTGCTC AGGAATCCTG GCTTCGTCCT GGCCCCACAG ACCTGCTGCT GGAGGTCGGA
    CCCAGGCTGA ACTTTTCCAC CCCGACATCC AGCAACATTG TGTCAGTGTG CCAGGCTGCA
    GGGCTGGGGG CTGTGGACCG CGTGGAGCCA ACCCGGCGCT ACCTGCTCTC GTTTGCCCAC
    CCACCTTCAG CTGACCTGGA GGCCATTGCC CTGGCTACCC TGCATGACCG GATGACAGAG
    CAGCACTTCC CCCAGCCCAT CCAGAGCTTC TCTTCAGGGT GCATCCCAGC ACCCCTGAGC
    GGCCCAATTG ACGTGCTGGC TGAGGGCCGG TCTGCCCTGG AGAAAGCCAA CCAGGAGCTA
    GGTCTGGCCC TAGACTCCTG GGACCTGGAT TTCTACACCA AGCGCTTCCA GGAGCTGCAG
    CGCAACCCCA GCACTGTGGA AGTCTTCGAC TTGGCTCAGT CCAATAGCGA ACACAGCCGA
    CACTGGTTCT TCAAGGGTCG ACTCCATGTG GATGGACAGG AGCTGCCACA CTCGCTGTTT
    GAGTCCATCA TGAGCACCCA GGCGTCCTCC AACCCTAACA ACGTCCTCAA GTTCTGTGAC
    AACAGCAGTG CCATCCAGGG GAAAGAAGTC CGATTCTTGC GGCCTAAGGA CCCCACACGG
    CCAAGCTCCT TCCAGCAATG TCAGGGGCTG AGACATGTTG TCTTCACAGC AGAGACACAC
    AACTTCCCTA CAGGAGTAGC CCCCTTCAGT GGTGCAGCCA CTGGCACAGG GGGCCGGATC
    CGAGATGTCC AGTGCACAGG CCGAGGGGCC CACGTGGTGG CTGGCACTGC TGGCTATTGC
    TTTGGAAACC TGCACATCCC AGGTTACAGT ATGCCCTGGG AGGATCCAAG CTTCCAGTAT
    CCAGGAAACT TTGCCCGACC CCTGGAGATT GCCATTGAGG CCAGTAATGG GGCTTCTGAC
    TACGGCAACA AGTTTGGGGA ACCAGTGCTT GCTGGCTTTG CCCGCTCCTT GGGCCTTCAG
    CTTCCGGACG GCCAGCGGCG TGAGTGGATC AAGCCCATAA TGTTTAGTGG AGGCATTGGG
    TCCATGGAAG CTGAGCATGT GAGCAAGGAG CCCCCAGAGA CAGGCATGGA TGTCGTGAAG
    ATTGGCGGTC CTGTCTACAG GATTGGAGTT GGGGGCGGAG CTGCTTCGTC TGTGCAGGTG
    CAAGGAGACA ACGCCAGTGA CCTGGATTTT GGGGCTGTGC AGCGGGGAGA CCCAGAGATG
    GAGCAGAAGA TGAACCGTGT GATCCGGGCT TGTGTGGAGG CCCCTGGGGG AAACCCCATC
    TGCAGCCTCC ATGACCAGGG TGCTGGTGGC AATGGCAATG TCCTGAAGGA GCTGAGTGAC
    CCGGCTGGAG CCGTCATTCA CACCAGCTGC TTCCAGCTGG GGGACCCAAC CCTGAATGCC
    CTGGAAATCT GGGGGGCCGA GTACCAGGAG TCGAACGCAC TTCTGCTGAG GCCCTCTGAC
    CGGGACTTCC TGAGTTGTGT CAGCGCCCGG GAACGCTGCC CAGTCTGCTT TGTGGGCACC
    ATCACTGGAG ACAGGAGAAT AGTGCTGGTG GATGATCGGG AGTGGCCTAT GGGCACAGGT
    GGCCAGGTGG ATGCGCCCCC CACTCCTCCC CCAACCCCTG TGGACCTGGA CCTGGACTGG
    GTGCTGGGCA AGATGCCCCA GAAGGAGTTC TTCCTCCAGA GGAGTCTCCC CTTGCTGCAG
    CCTCTGACTC TGCCCCCAGG GCTGAGCGTG CGCCAGGCTC TGGCGCGGGT CCTCAGGCTG
    CCTGCCGTGG CCAGCAAGCG CTACCTCACC AACAAGGTGG ACCGTTCTGT GGGCGGCCTG
    GTGGCCCAGC AGCAGTGTGT GGGACCCCTG CAGACCCCTC TGGCAGATGT GGCGGTTGTG
    GCACTGAGTC ACCAGGAGCT TGTAGGGGCC GCCACAGCCC TGGGAGAGCA GCCAGTCAAG
    AGCCTGCTGG ACCCGAAGGT TGCCGCCCGG CTGGCTGTGG CCGAAGCCCT CACCAACCTG
    GTATTTGCTC TGGTCACCGA CCTCCAGGAT GTGAAATGCA GCGGGAACTG GATGTGGGCA
    GCCAAGCTCC CAGGGGAGGG TGCAGCTCTG GCTGACGCCT GTGAGGCTAT GGTGGCAGTG
    ATGGCGGCCC TGGGTGTAGC GGTAGACGGT GGCAAGGACT CCCTCAGCAT GGCTGCCCGG
    GTTGGCTCTG AAACCGTGCG GGCTCCTGGG TCACTGGTCA TCTCAGCCTA TGCTGTCTGT
    TCAGACATTA CAGCCACTGT GACCCCAGAC CTCAAGCGTC CTGGGGGGAG AGGCCACCTG
    CTCTACGTGC CTCTGAGTCC TGGGCAGCAC CGGCTGGGGG GCACAGCTCT GGCTCAGTGC
    TTCTCCCAGC TTGGCGAGCA GCCTCCCGAC CTGGATGTTC CTGAGAACTT GGTGCGAGCC
    TTCAGCATCA CCCAGGGGCT GCTGAAAGAC CGCCTCCTCT GCTCAGGCCA TGATGTCAGT
    GATGGAGGTC TCATCACTTG CCTGCTAGAG ATGGCCTTTG CTGGGAATTG TGGGATAGAG
    GTGGACATCC CCGCACCTGG GGTCGATGCC CTGCCTGTGC TGTTCGCCGA GGAGCCAGGC
    CTGGTGCTGG AGGTGCAGGA GCCGGACCTC GCCCAAGTGC TGATGCGTTA CCGGAATGCT
    GGCCTCCACT GCCTAGAGCT GGGTCTCACA GGCGACACGG GGCCCCACGC CATGGTCCGC
    GTGTCGGTGA ACAGGACGGT GGTTCTGGAG GAACCTGTTG GGCAGCTACG AGCCCTCTGG
    GAGGAAACCA GTTTCCAGTT GGACCGGCTA CAGGCAAAAC CACACTGTGT GGCCCAGGAA
    GAGCAAGGGC TGAGGGAGCG GGCAGGGCCC ACCTTCTGCC TGCCGCCTAC CTTCCCCAGA
    GCTTCCGTGC CTTGTGAGCC TGGTGGTCCT GCCCCCCGAG TCGCCATCAT TCGGGAAGAG
    GGCAGTAATG GAGACCGGGA GATGGCAGAT GCCTTCCACT TGGCTGGATT TGAGGTGTGG
    GATGTAACCA TGCAGGACCT GTGCTCTGGA GCCATCGGGC TGGACACATT CCGCGGTGTG
    GCCTTCGTAG GCGGCTTCAG CTACGCGGAC GTCCTGGGCT CTGCCAAAGG CTGGGCAGCT
    GCTGTGACCT TTAACCCGCA GGCCGGGGCT GAGATGAAGC GCTTCCGCCA GCGGCCAGAC
    ACCTTCAGCC TGGGCGTGTG TAACGGCTGT CAGCTGCTGG CCCTGCTGGG CTGGGTGGGA
    GGCAGTTCTA GTGAGGAGGT GGTGGAGATG TGCCAGGAGT CCTGGCCGGC CCGGCCCGGT
    CTCCTGCTAC GCCACAACCT GTCTGGGCGC TTTGAGTCGC GCTGGGCTAG TGTGCGCGTG
    GGGCCCGGGC CAGCCCTGAT GCTGCGAGGG ATGGAAGGCG CGGTGCTGCC CGTGTGGAGC
    GCTCACGGGG AAGGTTACAT GGCATTTTCT TCTCCGGAAC TCCAAGCCCA GATGGAGGCC
    AGGGGCTTAG CTCCACTGCA CTGGGCAGAT GATGATGGGA ACCCCACGGA ACAGTACCCC
    CTGAATCCCA ACGGCTCCCC AGGGGGTGTC GCTGGCATCT GCTCCCCCGA TGGCCGCCAC
    TTGGCCCTCA TGCCTCACCC GGAGCGGTCT GTGAGGCCTT GGCAGTGGGC CTGGCGACCT
    CCTCCATTTG ACACTCTAAC CACCTCCCCC TGGCTCCAGC TCTTCATCAA TGCCCGGAAT
    TGGACCCAGG AATGCAGCTG CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa29748
    Clone ID Related Accession (Same CDS sequence) XM_010816030.2 , NM_001256564.1
    Accession Version NM_001256564.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4017bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-22
    Organism Bos taurus(cattle)
    Product phosphoribosylformylglycinamidine synthase
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DAAA02048815.1. On or before Feb 11, 2012 this sequence version replaced XM_598557.4, XM_002695870.1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN02822088, SAMN02822089 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTCCTCCG TCCTTCACTT CTATGTCCGC CCCTCTGGCC ACGAGAGGGC AGCCTCTGAA 
    TACATTCAAA GGAAGCTGCA AAGGGAACTG CCAGAGCTGC AGGGTGTCAA GACCGAGCAG
    TGCTACAATG TGAACTGGGC AGCGGAGTCC TTCCCGAGCA ACAAGGAGAT GAAGAAGCTG
    ACATGGCTAT TTGGCTGCCC CTTGTTACTG GACGATGTTG CTCAGGAATC CTGGCTTCGT
    CCTGGCCCCA CAGACCTGCT GCTGGAGGTC GGACCCAGGC TGAACTTTTC CACCCCGACA
    TCCAGCAACA TTGTGTCAGT GTGCCAGGCT GCAGGGCTGG GGGCTGTGGA CCGCGTGGAG
    CCAACCCGGC GCTACCTGCT CTCGTTTGCC CACCCACCTT CAGCTGACCT GGAGGCCATT
    GCCCTGGCTA CCCTGCATGA CCGGATGACA GAGCAGCACT TCCCCCAGCC CATCCAGAGC
    TTCTCTTCAG GGTGCATCCC AGCACCCCTG AGCGGCCCAA TTGACGTGCT GGCTGAGGGC
    CGGTCTGCCC TGGAGAAAGC CAACCAGGAG CTAGGTCTGG CCCTAGACTC CTGGGACCTG
    GATTTCTACA CCAAGCGCTT CCAGGAGCTG CAGCGCAACC CCAGCACTGT GGAAGTCTTC
    GACTTGGCTC AGTCCAATAG CGAACACAGC CGACACTGGT TCTTCAAGGG TCGACTCCAT
    GTGGATGGAC AGGAGCTGCC ACACTCGCTG TTTGAGTCCA TCATGAGCAC CCAGGCGTCC
    TCCAACCCTA ACAACGTCCT CAAGTTCTGT GACAACAGCA GTGCCATCCA GGGGAAAGAA
    GTCCGATTCT TGCGGCCTAA GGACCCCACA CGGCCAAGCT CCTTCCAGCA ATGTCAGGGG
    CTGAGACATG TTGTCTTCAC AGCAGAGACA CACAACTTCC CTACAGGAGT AGCCCCCTTC
    AGTGGTGCAG CCACTGGCAC AGGGGGCCGG ATCCGAGATG TCCAGTGCAC AGGCCGAGGG
    GCCCACGTGG TGGCTGGCAC TGCTGGCTAT TGCTTTGGAA ACCTGCACAT CCCAGGTTAC
    AGTATGCCCT GGGAGGATCC AAGCTTCCAG TATCCAGGAA ACTTTGCCCG ACCCCTGGAG
    ATTGCCATTG AGGCCAGTAA TGGGGCTTCT GACTACGGCA ACAAGTTTGG GGAACCAGTG
    CTTGCTGGCT TTGCCCGCTC CTTGGGCCTT CAGCTTCCGG ACGGCCAGCG GCGTGAGTGG
    ATCAAGCCCA TAATGTTTAG TGGAGGCATT GGGTCCATGG AAGCTGAGCA TGTGAGCAAG
    GAGCCCCCAG AGACAGGCAT GGATGTCGTG AAGATTGGCG GTCCTGTCTA CAGGATTGGA
    GTTGGGGGCG GAGCTGCTTC GTCTGTGCAG GTGCAAGGAG ACAACGCCAG TGACCTGGAT
    TTTGGGGCTG TGCAGCGGGG AGACCCAGAG ATGGAGCAGA AGATGAACCG TGTGATCCGG
    GCTTGTGTGG AGGCCCCTGG GGGAAACCCC ATCTGCAGCC TCCATGACCA GGGTGCTGGT
    GGCAATGGCA ATGTCCTGAA GGAGCTGAGT GACCCGGCTG GAGCCGTCAT TCACACCAGC
    TGCTTCCAGC TGGGGGACCC AACCCTGAAT GCCCTGGAAA TCTGGGGGGC CGAGTACCAG
    GAGTCGAACG CACTTCTGCT GAGGCCCTCT GACCGGGACT TCCTGAGTTG TGTCAGCGCC
    CGGGAACGCT GCCCAGTCTG CTTTGTGGGC ACCATCACTG GAGACAGGAG AATAGTGCTG
    GTGGATGATC GGGAGTGGCC TATGGGCACA GGTGGCCAGG TGGATGCGCC CCCCACTCCT
    CCCCCAACCC CTGTGGACCT GGACCTGGAC TGGGTGCTGG GCAAGATGCC CCAGAAGGAG
    TTCTTCCTCC AGAGGAGTCT CCCCTTGCTG CAGCCTCTGA CTCTGCCCCC AGGGCTGAGC
    GTGCGCCAGG CTCTGGCGCG GGTCCTCAGG CTGCCTGCCG TGGCCAGCAA GCGCTACCTC
    ACCAACAAGG TGGACCGTTC TGTGGGCGGC CTGGTGGCCC AGCAGCAGTG TGTGGGACCC
    CTGCAGACCC CTCTGGCAGA TGTGGCGGTT GTGGCACTGA GTCACCAGGA GCTTGTAGGG
    GCCGCCACAG CCCTGGGAGA GCAGCCAGTC AAGAGCCTGC TGGACCCGAA GGTTGCCGCC
    CGGCTGGCTG TGGCCGAAGC CCTCACCAAC CTGGTATTTG CTCTGGTCAC CGACCTCCAG
    GATGTGAAAT GCAGCGGGAA CTGGATGTGG GCAGCCAAGC TCCCAGGGGA GGGTGCAGCT
    CTGGCTGACG CCTGTGAGGC TATGGTGGCA GTGATGGCGG CCCTGGGTGT AGCGGTAGAC
    GGTGGCAAGG ACTCCCTCAG CATGGCTGCC CGGGTTGGCT CTGAAACCGT GCGGGCTCCT
    GGGTCACTGG TCATCTCAGC CTATGCTGTC TGTTCAGACA TTACAGCCAC TGTGACCCCA
    GACCTCAAGC GTCCTGGGGG GAGAGGCCAC CTGCTCTACG TGCCTCTGAG TCCTGGGCAG
    CACCGGCTGG GGGGCACAGC TCTGGCTCAG TGCTTCTCCC AGCTTGGCGA GCAGCCTCCC
    GACCTGGATG TTCCTGAGAA CTTGGTGCGA GCCTTCAGCA TCACCCAGGG GCTGCTGAAA
    GACCGCCTCC TCTGCTCAGG CCATGATGTC AGTGATGGAG GTCTCATCAC TTGCCTGCTA
    GAGATGGCCT TTGCTGGGAA TTGTGGGATA GAGGTGGACA TCCCCGCACC TGGGGTCGAT
    GCCCTGCCTG TGCTGTTCGC CGAGGAGCCA GGCCTGGTGC TGGAGGTGCA GGAGCCGGAC
    CTCGCCCAAG TGCTGATGCG TTACCGGAAT GCTGGCCTCC ACTGCCTAGA GCTGGGTCTC
    ACAGGCGACA CGGGGCCCCA CGCCATGGTC CGCGTGTCGG TGAACAGGAC GGTGGTTCTG
    GAGGAACCTG TTGGGCAGCT ACGAGCCCTC TGGGAGGAAA CCAGTTTCCA GTTGGACCGG
    CTACAGGCAA AACCACACTG TGTGGCCCAG GAAGAGCAAG GGCTGAGGGA GCGGGCAGGG
    CCCACCTTCT GCCTGCCGCC TACCTTCCCC AGAGCTTCCG TGCCTTGTGA GCCTGGTGGT
    CCTGCCCCCC GAGTCGCCAT CATTCGGGAA GAGGGCAGTA ATGGAGACCG GGAGATGGCA
    GATGCCTTCC ACTTGGCTGG ATTTGAGGTG TGGGATGTAA CCATGCAGGA CCTGTGCTCT
    GGAGCCATCG GGCTGGACAC ATTCCGCGGT GTGGCCTTCG TAGGCGGCTT CAGCTACGCG
    GACGTCCTGG GCTCTGCCAA AGGCTGGGCA GCTGCTGTGA CCTTTAACCC GCAGGCCGGG
    GCTGAGATGA AGCGCTTCCG CCAGCGGCCA GACACCTTCA GCCTGGGCGT GTGTAACGGC
    TGTCAGCTGC TGGCCCTGCT GGGCTGGGTG GGAGGCAGTT CTAGTGAGGA GGTGGTGGAG
    ATGTGCCAGG AGTCCTGGCC GGCCCGGCCC GGTCTCCTGC TACGCCACAA CCTGTCTGGG
    CGCTTTGAGT CGCGCTGGGC TAGTGTGCGC GTGGGGCCCG GGCCAGCCCT GATGCTGCGA
    GGGATGGAAG GCGCGGTGCT GCCCGTGTGG AGCGCTCACG GGGAAGGTTA CATGGCATTT
    TCTTCTCCGG AACTCCAAGC CCAGATGGAG GCCAGGGGCT TAGCTCCACT GCACTGGGCA
    GATGATGATG GGAACCCCAC GGAACAGTAC CCCCTGAATC CCAACGGCTC CCCAGGGGGT
    GTCGCTGGCA TCTGCTCCCC CGATGGCCGC CACTTGGCCC TCATGCCTCA CCCGGAGCGG
    TCTGTGAGGC CTTGGCAGTG GGCCTGGCGA CCTCCTCCAT TTGACACTCT AACCACCTCC
    CCCTGGCTCC AGCTCTTCAT CAATGCCCGG AATTGGACCC AGGAATGCAG CTGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001243493.1
    CDS1..4017
    Translation

    Target ORF information:

    RefSeq Version NM_001256564.1
    Organism Bos taurus(cattle)
    Definition Bos taurus phosphoribosylformylglycinamidine synthase (PFAS), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001256564.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTCCTCCG TCCTTCACTT CTATGTCCGC CCCTCTGGCC ACGAGAGGGC AGCCTCTGAA 
    TACATTCAAA GGAAGCTGCA AAGGGAACTG CCAGAGCTGC AGGGTGTCAA GACCGAGCAG
    TGCTACAATG TGAACTGGGC AGCGGAGTCC TTCCCGAGCA ACAAGGAGAT GAAGAAGCTG
    ACATGGCTAT TTGGCTGCCC CTTGTTACTG GACGATGTTG CTCAGGAATC CTGGCTTCGT
    CCTGGCCCCA CAGACCTGCT GCTGGAGGTC GGACCCAGGC TGAACTTTTC CACCCCGACA
    TCCAGCAACA TTGTGTCAGT GTGCCAGGCT GCAGGGCTGG GGGCTGTGGA CCGCGTGGAG
    CCAACCCGGC GCTACCTGCT CTCGTTTGCC CACCCACCTT CAGCTGACCT GGAGGCCATT
    GCCCTGGCTA CCCTGCATGA CCGGATGACA GAGCAGCACT TCCCCCAGCC CATCCAGAGC
    TTCTCTTCAG GGTGCATCCC AGCACCCCTG AGCGGCCCAA TTGACGTGCT GGCTGAGGGC
    CGGTCTGCCC TGGAGAAAGC CAACCAGGAG CTAGGTCTGG CCCTAGACTC CTGGGACCTG
    GATTTCTACA CCAAGCGCTT CCAGGAGCTG CAGCGCAACC CCAGCACTGT GGAAGTCTTC
    GACTTGGCTC AGTCCAATAG CGAACACAGC CGACACTGGT TCTTCAAGGG TCGACTCCAT
    GTGGATGGAC AGGAGCTGCC ACACTCGCTG TTTGAGTCCA TCATGAGCAC CCAGGCGTCC
    TCCAACCCTA ACAACGTCCT CAAGTTCTGT GACAACAGCA GTGCCATCCA GGGGAAAGAA
    GTCCGATTCT TGCGGCCTAA GGACCCCACA CGGCCAAGCT CCTTCCAGCA ATGTCAGGGG
    CTGAGACATG TTGTCTTCAC AGCAGAGACA CACAACTTCC CTACAGGAGT AGCCCCCTTC
    AGTGGTGCAG CCACTGGCAC AGGGGGCCGG ATCCGAGATG TCCAGTGCAC AGGCCGAGGG
    GCCCACGTGG TGGCTGGCAC TGCTGGCTAT TGCTTTGGAA ACCTGCACAT CCCAGGTTAC
    AGTATGCCCT GGGAGGATCC AAGCTTCCAG TATCCAGGAA ACTTTGCCCG ACCCCTGGAG
    ATTGCCATTG AGGCCAGTAA TGGGGCTTCT GACTACGGCA ACAAGTTTGG GGAACCAGTG
    CTTGCTGGCT TTGCCCGCTC CTTGGGCCTT CAGCTTCCGG ACGGCCAGCG GCGTGAGTGG
    ATCAAGCCCA TAATGTTTAG TGGAGGCATT GGGTCCATGG AAGCTGAGCA TGTGAGCAAG
    GAGCCCCCAG AGACAGGCAT GGATGTCGTG AAGATTGGCG GTCCTGTCTA CAGGATTGGA
    GTTGGGGGCG GAGCTGCTTC GTCTGTGCAG GTGCAAGGAG ACAACGCCAG TGACCTGGAT
    TTTGGGGCTG TGCAGCGGGG AGACCCAGAG ATGGAGCAGA AGATGAACCG TGTGATCCGG
    GCTTGTGTGG AGGCCCCTGG GGGAAACCCC ATCTGCAGCC TCCATGACCA GGGTGCTGGT
    GGCAATGGCA ATGTCCTGAA GGAGCTGAGT GACCCGGCTG GAGCCGTCAT TCACACCAGC
    TGCTTCCAGC TGGGGGACCC AACCCTGAAT GCCCTGGAAA TCTGGGGGGC CGAGTACCAG
    GAGTCGAACG CACTTCTGCT GAGGCCCTCT GACCGGGACT TCCTGAGTTG TGTCAGCGCC
    CGGGAACGCT GCCCAGTCTG CTTTGTGGGC ACCATCACTG GAGACAGGAG AATAGTGCTG
    GTGGATGATC GGGAGTGGCC TATGGGCACA GGTGGCCAGG TGGATGCGCC CCCCACTCCT
    CCCCCAACCC CTGTGGACCT GGACCTGGAC TGGGTGCTGG GCAAGATGCC CCAGAAGGAG
    TTCTTCCTCC AGAGGAGTCT CCCCTTGCTG CAGCCTCTGA CTCTGCCCCC AGGGCTGAGC
    GTGCGCCAGG CTCTGGCGCG GGTCCTCAGG CTGCCTGCCG TGGCCAGCAA GCGCTACCTC
    ACCAACAAGG TGGACCGTTC TGTGGGCGGC CTGGTGGCCC AGCAGCAGTG TGTGGGACCC
    CTGCAGACCC CTCTGGCAGA TGTGGCGGTT GTGGCACTGA GTCACCAGGA GCTTGTAGGG
    GCCGCCACAG CCCTGGGAGA GCAGCCAGTC AAGAGCCTGC TGGACCCGAA GGTTGCCGCC
    CGGCTGGCTG TGGCCGAAGC CCTCACCAAC CTGGTATTTG CTCTGGTCAC CGACCTCCAG
    GATGTGAAAT GCAGCGGGAA CTGGATGTGG GCAGCCAAGC TCCCAGGGGA GGGTGCAGCT
    CTGGCTGACG CCTGTGAGGC TATGGTGGCA GTGATGGCGG CCCTGGGTGT AGCGGTAGAC
    GGTGGCAAGG ACTCCCTCAG CATGGCTGCC CGGGTTGGCT CTGAAACCGT GCGGGCTCCT
    GGGTCACTGG TCATCTCAGC CTATGCTGTC TGTTCAGACA TTACAGCCAC TGTGACCCCA
    GACCTCAAGC GTCCTGGGGG GAGAGGCCAC CTGCTCTACG TGCCTCTGAG TCCTGGGCAG
    CACCGGCTGG GGGGCACAGC TCTGGCTCAG TGCTTCTCCC AGCTTGGCGA GCAGCCTCCC
    GACCTGGATG TTCCTGAGAA CTTGGTGCGA GCCTTCAGCA TCACCCAGGG GCTGCTGAAA
    GACCGCCTCC TCTGCTCAGG CCATGATGTC AGTGATGGAG GTCTCATCAC TTGCCTGCTA
    GAGATGGCCT TTGCTGGGAA TTGTGGGATA GAGGTGGACA TCCCCGCACC TGGGGTCGAT
    GCCCTGCCTG TGCTGTTCGC CGAGGAGCCA GGCCTGGTGC TGGAGGTGCA GGAGCCGGAC
    CTCGCCCAAG TGCTGATGCG TTACCGGAAT GCTGGCCTCC ACTGCCTAGA GCTGGGTCTC
    ACAGGCGACA CGGGGCCCCA CGCCATGGTC CGCGTGTCGG TGAACAGGAC GGTGGTTCTG
    GAGGAACCTG TTGGGCAGCT ACGAGCCCTC TGGGAGGAAA CCAGTTTCCA GTTGGACCGG
    CTACAGGCAA AACCACACTG TGTGGCCCAG GAAGAGCAAG GGCTGAGGGA GCGGGCAGGG
    CCCACCTTCT GCCTGCCGCC TACCTTCCCC AGAGCTTCCG TGCCTTGTGA GCCTGGTGGT
    CCTGCCCCCC GAGTCGCCAT CATTCGGGAA GAGGGCAGTA ATGGAGACCG GGAGATGGCA
    GATGCCTTCC ACTTGGCTGG ATTTGAGGTG TGGGATGTAA CCATGCAGGA CCTGTGCTCT
    GGAGCCATCG GGCTGGACAC ATTCCGCGGT GTGGCCTTCG TAGGCGGCTT CAGCTACGCG
    GACGTCCTGG GCTCTGCCAA AGGCTGGGCA GCTGCTGTGA CCTTTAACCC GCAGGCCGGG
    GCTGAGATGA AGCGCTTCCG CCAGCGGCCA GACACCTTCA GCCTGGGCGT GTGTAACGGC
    TGTCAGCTGC TGGCCCTGCT GGGCTGGGTG GGAGGCAGTT CTAGTGAGGA GGTGGTGGAG
    ATGTGCCAGG AGTCCTGGCC GGCCCGGCCC GGTCTCCTGC TACGCCACAA CCTGTCTGGG
    CGCTTTGAGT CGCGCTGGGC TAGTGTGCGC GTGGGGCCCG GGCCAGCCCT GATGCTGCGA
    GGGATGGAAG GCGCGGTGCT GCCCGTGTGG AGCGCTCACG GGGAAGGTTA CATGGCATTT
    TCTTCTCCGG AACTCCAAGC CCAGATGGAG GCCAGGGGCT TAGCTCCACT GCACTGGGCA
    GATGATGATG GGAACCCCAC GGAACAGTAC CCCCTGAATC CCAACGGCTC CCCAGGGGGT
    GTCGCTGGCA TCTGCTCCCC CGATGGCCGC CACTTGGCCC TCATGCCTCA CCCGGAGCGG
    TCTGTGAGGC CTTGGCAGTG GGCCTGGCGA CCTCCTCCAT TTGACACTCT AACCACCTCC
    CCCTGGCTCC AGCTCTTCAT CAATGCCCGG AATTGGACCC AGGAATGCAG CTGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa29748
    Clone ID Related Accession (Same CDS sequence) XM_010816030.2 , NM_001256564.1
    Accession Version XM_010816030.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4017bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product phosphoribosylformylglycinamidine synthase isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104494.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010816030.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTCCTCCG TCCTTCACTT CTATGTCCGC CCCTCTGGCC ACGAGAGGGC AGCCTCTGAA 
    TACATTCAAA GGAAGCTGCA AAGGGAACTG CCAGAGCTGC AGGGTGTCAA GACCGAGCAG
    TGCTACAATG TGAACTGGGC AGCGGAGTCC TTCCCGAGCA ACAAGGAGAT GAAGAAGCTG
    ACATGGCTAT TTGGCTGCCC CTTGTTACTG GACGATGTTG CTCAGGAATC CTGGCTTCGT
    CCTGGCCCCA CAGACCTGCT GCTGGAGGTC GGACCCAGGC TGAACTTTTC CACCCCGACA
    TCCAGCAACA TTGTGTCAGT GTGCCAGGCT GCAGGGCTGG GGGCTGTGGA CCGCGTGGAG
    CCAACCCGGC GCTACCTGCT CTCGTTTGCC CACCCACCTT CAGCTGACCT GGAGGCCATT
    GCCCTGGCTA CCCTGCATGA CCGGATGACA GAGCAGCACT TCCCCCAGCC CATCCAGAGC
    TTCTCTTCAG GGTGCATCCC AGCACCCCTG AGCGGCCCAA TTGACGTGCT GGCTGAGGGC
    CGGTCTGCCC TGGAGAAAGC CAACCAGGAG CTAGGTCTGG CCCTAGACTC CTGGGACCTG
    GATTTCTACA CCAAGCGCTT CCAGGAGCTG CAGCGCAACC CCAGCACTGT GGAAGTCTTC
    GACTTGGCTC AGTCCAATAG CGAACACAGC CGACACTGGT TCTTCAAGGG TCGACTCCAT
    GTGGATGGAC AGGAGCTGCC ACACTCGCTG TTTGAGTCCA TCATGAGCAC CCAGGCGTCC
    TCCAACCCTA ACAACGTCCT CAAGTTCTGT GACAACAGCA GTGCCATCCA GGGGAAAGAA
    GTCCGATTCT TGCGGCCTAA GGACCCCACA CGGCCAAGCT CCTTCCAGCA ATGTCAGGGG
    CTGAGACATG TTGTCTTCAC AGCAGAGACA CACAACTTCC CTACAGGAGT AGCCCCCTTC
    AGTGGTGCAG CCACTGGCAC AGGGGGCCGG ATCCGAGATG TCCAGTGCAC AGGCCGAGGG
    GCCCACGTGG TGGCTGGCAC TGCTGGCTAT TGCTTTGGAA ACCTGCACAT CCCAGGTTAC
    AGTATGCCCT GGGAGGATCC AAGCTTCCAG TATCCAGGAA ACTTTGCCCG ACCCCTGGAG
    ATTGCCATTG AGGCCAGTAA TGGGGCTTCT GACTACGGCA ACAAGTTTGG GGAACCAGTG
    CTTGCTGGCT TTGCCCGCTC CTTGGGCCTT CAGCTTCCGG ACGGCCAGCG GCGTGAGTGG
    ATCAAGCCCA TAATGTTTAG TGGAGGCATT GGGTCCATGG AAGCTGAGCA TGTGAGCAAG
    GAGCCCCCAG AGACAGGCAT GGATGTCGTG AAGATTGGCG GTCCTGTCTA CAGGATTGGA
    GTTGGGGGCG GAGCTGCTTC GTCTGTGCAG GTGCAAGGAG ACAACGCCAG TGACCTGGAT
    TTTGGGGCTG TGCAGCGGGG AGACCCAGAG ATGGAGCAGA AGATGAACCG TGTGATCCGG
    GCTTGTGTGG AGGCCCCTGG GGGAAACCCC ATCTGCAGCC TCCATGACCA GGGTGCTGGT
    GGCAATGGCA ATGTCCTGAA GGAGCTGAGT GACCCGGCTG GAGCCGTCAT TCACACCAGC
    TGCTTCCAGC TGGGGGACCC AACCCTGAAT GCCCTGGAAA TCTGGGGGGC CGAGTACCAG
    GAGTCGAACG CACTTCTGCT GAGGCCCTCT GACCGGGACT TCCTGAGTTG TGTCAGCGCC
    CGGGAACGCT GCCCAGTCTG CTTTGTGGGC ACCATCACTG GAGACAGGAG AATAGTGCTG
    GTGGATGATC GGGAGTGGCC TATGGGCACA GGTGGCCAGG TGGATGCGCC CCCCACTCCT
    CCCCCAACCC CTGTGGACCT GGACCTGGAC TGGGTGCTGG GCAAGATGCC CCAGAAGGAG
    TTCTTCCTCC AGAGGAGTCT CCCCTTGCTG CAGCCTCTGA CTCTGCCCCC AGGGCTGAGC
    GTGCGCCAGG CTCTGGCGCG GGTCCTCAGG CTGCCTGCCG TGGCCAGCAA GCGCTACCTC
    ACCAACAAGG TGGACCGTTC TGTGGGCGGC CTGGTGGCCC AGCAGCAGTG TGTGGGACCC
    CTGCAGACCC CTCTGGCAGA TGTGGCGGTT GTGGCACTGA GTCACCAGGA GCTTGTAGGG
    GCCGCCACAG CCCTGGGAGA GCAGCCAGTC AAGAGCCTGC TGGACCCGAA GGTTGCCGCC
    CGGCTGGCTG TGGCCGAAGC CCTCACCAAC CTGGTATTTG CTCTGGTCAC CGACCTCCAG
    GATGTGAAAT GCAGCGGGAA CTGGATGTGG GCAGCCAAGC TCCCAGGGGA GGGTGCAGCT
    CTGGCTGACG CCTGTGAGGC TATGGTGGCA GTGATGGCGG CCCTGGGTGT AGCGGTAGAC
    GGTGGCAAGG ACTCCCTCAG CATGGCTGCC CGGGTTGGCT CTGAAACCGT GCGGGCTCCT
    GGGTCACTGG TCATCTCAGC CTATGCTGTC TGTTCAGACA TTACAGCCAC TGTGACCCCA
    GACCTCAAGC GTCCTGGGGG GAGAGGCCAC CTGCTCTACG TGCCTCTGAG TCCTGGGCAG
    CACCGGCTGG GGGGCACAGC TCTGGCTCAG TGCTTCTCCC AGCTTGGCGA GCAGCCTCCC
    GACCTGGATG TTCCTGAGAA CTTGGTGCGA GCCTTCAGCA TCACCCAGGG GCTGCTGAAA
    GACCGCCTCC TCTGCTCAGG CCATGATGTC AGTGATGGAG GTCTCATCAC TTGCCTGCTA
    GAGATGGCCT TTGCTGGGAA TTGTGGGATA GAGGTGGACA TCCCCGCACC TGGGGTCGAT
    GCCCTGCCTG TGCTGTTCGC CGAGGAGCCA GGCCTGGTGC TGGAGGTGCA GGAGCCGGAC
    CTCGCCCAAG TGCTGATGCG TTACCGGAAT GCTGGCCTCC ACTGCCTAGA GCTGGGTCTC
    ACAGGCGACA CGGGGCCCCA CGCCATGGTC CGCGTGTCGG TGAACAGGAC GGTGGTTCTG
    GAGGAACCTG TTGGGCAGCT ACGAGCCCTC TGGGAGGAAA CCAGTTTCCA GTTGGACCGG
    CTACAGGCAA AACCACACTG TGTGGCCCAG GAAGAGCAAG GGCTGAGGGA GCGGGCAGGG
    CCCACCTTCT GCCTGCCGCC TACCTTCCCC AGAGCTTCCG TGCCTTGTGA GCCTGGTGGT
    CCTGCCCCCC GAGTCGCCAT CATTCGGGAA GAGGGCAGTA ATGGAGACCG GGAGATGGCA
    GATGCCTTCC ACTTGGCTGG ATTTGAGGTG TGGGATGTAA CCATGCAGGA CCTGTGCTCT
    GGAGCCATCG GGCTGGACAC ATTCCGCGGT GTGGCCTTCG TAGGCGGCTT CAGCTACGCG
    GACGTCCTGG GCTCTGCCAA AGGCTGGGCA GCTGCTGTGA CCTTTAACCC GCAGGCCGGG
    GCTGAGATGA AGCGCTTCCG CCAGCGGCCA GACACCTTCA GCCTGGGCGT GTGTAACGGC
    TGTCAGCTGC TGGCCCTGCT GGGCTGGGTG GGAGGCAGTT CTAGTGAGGA GGTGGTGGAG
    ATGTGCCAGG AGTCCTGGCC GGCCCGGCCC GGTCTCCTGC TACGCCACAA CCTGTCTGGG
    CGCTTTGAGT CGCGCTGGGC TAGTGTGCGC GTGGGGCCCG GGCCAGCCCT GATGCTGCGA
    GGGATGGAAG GCGCGGTGCT GCCCGTGTGG AGCGCTCACG GGGAAGGTTA CATGGCATTT
    TCTTCTCCGG AACTCCAAGC CCAGATGGAG GCCAGGGGCT TAGCTCCACT GCACTGGGCA
    GATGATGATG GGAACCCCAC GGAACAGTAC CCCCTGAATC CCAACGGCTC CCCAGGGGGT
    GTCGCTGGCA TCTGCTCCCC CGATGGCCGC CACTTGGCCC TCATGCCTCA CCCGGAGCGG
    TCTGTGAGGC CTTGGCAGTG GGCCTGGCGA CCTCCTCCAT TTGACACTCT AACCACCTCC
    CCCTGGCTCC AGCTCTTCAT CAATGCCCGG AATTGGACCC AGGAATGCAG CTGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010814332.1
    CDS1030..5046
    Translation

    Target ORF information:

    RefSeq Version XM_010816030.2
    Organism Bos taurus(cattle)
    Definition Bos taurus phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010816030.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTCCTCCG TCCTTCACTT CTATGTCCGC CCCTCTGGCC ACGAGAGGGC AGCCTCTGAA 
    TACATTCAAA GGAAGCTGCA AAGGGAACTG CCAGAGCTGC AGGGTGTCAA GACCGAGCAG
    TGCTACAATG TGAACTGGGC AGCGGAGTCC TTCCCGAGCA ACAAGGAGAT GAAGAAGCTG
    ACATGGCTAT TTGGCTGCCC CTTGTTACTG GACGATGTTG CTCAGGAATC CTGGCTTCGT
    CCTGGCCCCA CAGACCTGCT GCTGGAGGTC GGACCCAGGC TGAACTTTTC CACCCCGACA
    TCCAGCAACA TTGTGTCAGT GTGCCAGGCT GCAGGGCTGG GGGCTGTGGA CCGCGTGGAG
    CCAACCCGGC GCTACCTGCT CTCGTTTGCC CACCCACCTT CAGCTGACCT GGAGGCCATT
    GCCCTGGCTA CCCTGCATGA CCGGATGACA GAGCAGCACT TCCCCCAGCC CATCCAGAGC
    TTCTCTTCAG GGTGCATCCC AGCACCCCTG AGCGGCCCAA TTGACGTGCT GGCTGAGGGC
    CGGTCTGCCC TGGAGAAAGC CAACCAGGAG CTAGGTCTGG CCCTAGACTC CTGGGACCTG
    GATTTCTACA CCAAGCGCTT CCAGGAGCTG CAGCGCAACC CCAGCACTGT GGAAGTCTTC
    GACTTGGCTC AGTCCAATAG CGAACACAGC CGACACTGGT TCTTCAAGGG TCGACTCCAT
    GTGGATGGAC AGGAGCTGCC ACACTCGCTG TTTGAGTCCA TCATGAGCAC CCAGGCGTCC
    TCCAACCCTA ACAACGTCCT CAAGTTCTGT GACAACAGCA GTGCCATCCA GGGGAAAGAA
    GTCCGATTCT TGCGGCCTAA GGACCCCACA CGGCCAAGCT CCTTCCAGCA ATGTCAGGGG
    CTGAGACATG TTGTCTTCAC AGCAGAGACA CACAACTTCC CTACAGGAGT AGCCCCCTTC
    AGTGGTGCAG CCACTGGCAC AGGGGGCCGG ATCCGAGATG TCCAGTGCAC AGGCCGAGGG
    GCCCACGTGG TGGCTGGCAC TGCTGGCTAT TGCTTTGGAA ACCTGCACAT CCCAGGTTAC
    AGTATGCCCT GGGAGGATCC AAGCTTCCAG TATCCAGGAA ACTTTGCCCG ACCCCTGGAG
    ATTGCCATTG AGGCCAGTAA TGGGGCTTCT GACTACGGCA ACAAGTTTGG GGAACCAGTG
    CTTGCTGGCT TTGCCCGCTC CTTGGGCCTT CAGCTTCCGG ACGGCCAGCG GCGTGAGTGG
    ATCAAGCCCA TAATGTTTAG TGGAGGCATT GGGTCCATGG AAGCTGAGCA TGTGAGCAAG
    GAGCCCCCAG AGACAGGCAT GGATGTCGTG AAGATTGGCG GTCCTGTCTA CAGGATTGGA
    GTTGGGGGCG GAGCTGCTTC GTCTGTGCAG GTGCAAGGAG ACAACGCCAG TGACCTGGAT
    TTTGGGGCTG TGCAGCGGGG AGACCCAGAG ATGGAGCAGA AGATGAACCG TGTGATCCGG
    GCTTGTGTGG AGGCCCCTGG GGGAAACCCC ATCTGCAGCC TCCATGACCA GGGTGCTGGT
    GGCAATGGCA ATGTCCTGAA GGAGCTGAGT GACCCGGCTG GAGCCGTCAT TCACACCAGC
    TGCTTCCAGC TGGGGGACCC AACCCTGAAT GCCCTGGAAA TCTGGGGGGC CGAGTACCAG
    GAGTCGAACG CACTTCTGCT GAGGCCCTCT GACCGGGACT TCCTGAGTTG TGTCAGCGCC
    CGGGAACGCT GCCCAGTCTG CTTTGTGGGC ACCATCACTG GAGACAGGAG AATAGTGCTG
    GTGGATGATC GGGAGTGGCC TATGGGCACA GGTGGCCAGG TGGATGCGCC CCCCACTCCT
    CCCCCAACCC CTGTGGACCT GGACCTGGAC TGGGTGCTGG GCAAGATGCC CCAGAAGGAG
    TTCTTCCTCC AGAGGAGTCT CCCCTTGCTG CAGCCTCTGA CTCTGCCCCC AGGGCTGAGC
    GTGCGCCAGG CTCTGGCGCG GGTCCTCAGG CTGCCTGCCG TGGCCAGCAA GCGCTACCTC
    ACCAACAAGG TGGACCGTTC TGTGGGCGGC CTGGTGGCCC AGCAGCAGTG TGTGGGACCC
    CTGCAGACCC CTCTGGCAGA TGTGGCGGTT GTGGCACTGA GTCACCAGGA GCTTGTAGGG
    GCCGCCACAG CCCTGGGAGA GCAGCCAGTC AAGAGCCTGC TGGACCCGAA GGTTGCCGCC
    CGGCTGGCTG TGGCCGAAGC CCTCACCAAC CTGGTATTTG CTCTGGTCAC CGACCTCCAG
    GATGTGAAAT GCAGCGGGAA CTGGATGTGG GCAGCCAAGC TCCCAGGGGA GGGTGCAGCT
    CTGGCTGACG CCTGTGAGGC TATGGTGGCA GTGATGGCGG CCCTGGGTGT AGCGGTAGAC
    GGTGGCAAGG ACTCCCTCAG CATGGCTGCC CGGGTTGGCT CTGAAACCGT GCGGGCTCCT
    GGGTCACTGG TCATCTCAGC CTATGCTGTC TGTTCAGACA TTACAGCCAC TGTGACCCCA
    GACCTCAAGC GTCCTGGGGG GAGAGGCCAC CTGCTCTACG TGCCTCTGAG TCCTGGGCAG
    CACCGGCTGG GGGGCACAGC TCTGGCTCAG TGCTTCTCCC AGCTTGGCGA GCAGCCTCCC
    GACCTGGATG TTCCTGAGAA CTTGGTGCGA GCCTTCAGCA TCACCCAGGG GCTGCTGAAA
    GACCGCCTCC TCTGCTCAGG CCATGATGTC AGTGATGGAG GTCTCATCAC TTGCCTGCTA
    GAGATGGCCT TTGCTGGGAA TTGTGGGATA GAGGTGGACA TCCCCGCACC TGGGGTCGAT
    GCCCTGCCTG TGCTGTTCGC CGAGGAGCCA GGCCTGGTGC TGGAGGTGCA GGAGCCGGAC
    CTCGCCCAAG TGCTGATGCG TTACCGGAAT GCTGGCCTCC ACTGCCTAGA GCTGGGTCTC
    ACAGGCGACA CGGGGCCCCA CGCCATGGTC CGCGTGTCGG TGAACAGGAC GGTGGTTCTG
    GAGGAACCTG TTGGGCAGCT ACGAGCCCTC TGGGAGGAAA CCAGTTTCCA GTTGGACCGG
    CTACAGGCAA AACCACACTG TGTGGCCCAG GAAGAGCAAG GGCTGAGGGA GCGGGCAGGG
    CCCACCTTCT GCCTGCCGCC TACCTTCCCC AGAGCTTCCG TGCCTTGTGA GCCTGGTGGT
    CCTGCCCCCC GAGTCGCCAT CATTCGGGAA GAGGGCAGTA ATGGAGACCG GGAGATGGCA
    GATGCCTTCC ACTTGGCTGG ATTTGAGGTG TGGGATGTAA CCATGCAGGA CCTGTGCTCT
    GGAGCCATCG GGCTGGACAC ATTCCGCGGT GTGGCCTTCG TAGGCGGCTT CAGCTACGCG
    GACGTCCTGG GCTCTGCCAA AGGCTGGGCA GCTGCTGTGA CCTTTAACCC GCAGGCCGGG
    GCTGAGATGA AGCGCTTCCG CCAGCGGCCA GACACCTTCA GCCTGGGCGT GTGTAACGGC
    TGTCAGCTGC TGGCCCTGCT GGGCTGGGTG GGAGGCAGTT CTAGTGAGGA GGTGGTGGAG
    ATGTGCCAGG AGTCCTGGCC GGCCCGGCCC GGTCTCCTGC TACGCCACAA CCTGTCTGGG
    CGCTTTGAGT CGCGCTGGGC TAGTGTGCGC GTGGGGCCCG GGCCAGCCCT GATGCTGCGA
    GGGATGGAAG GCGCGGTGCT GCCCGTGTGG AGCGCTCACG GGGAAGGTTA CATGGCATTT
    TCTTCTCCGG AACTCCAAGC CCAGATGGAG GCCAGGGGCT TAGCTCCACT GCACTGGGCA
    GATGATGATG GGAACCCCAC GGAACAGTAC CCCCTGAATC CCAACGGCTC CCCAGGGGGT
    GTCGCTGGCA TCTGCTCCCC CGATGGCCGC CACTTGGCCC TCATGCCTCA CCCGGAGCGG
    TCTGTGAGGC CTTGGCAGTG GGCCTGGCGA CCTCCTCCAT TTGACACTCT AACCACCTCC
    CCCTGGCTCC AGCTCTTCAT CAATGCCCGG AATTGGACCC AGGAATGCAG CTGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.