Pfas cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pfas
Entrez Gene ID	237823
Full Name	phosphoribosylformylglycinamidine synthase (FGAR amidotransferase)
Synonyms	4432409B16Rik,FGAMS,FGAR-AT,FGARAT,Gm18,PURL,Sofa
General protein information	Preferred Names phosphoribosylformylglycinamidine synthase (FGAR amidotransferase) Names phosphoribosylformylglycinamidine synthase FGAM synthase FGAR amidotransferase formylglycinamide ribonucleotide amidotransferase formylglycinamide ribotide amidotransferase formylglycinamide ribotide synthetase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 B3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001159519.1	NP_001152991.1	phosphoribosylformylglycinamidine synthase

Pathways from BioSystems

Homologies

	PFAS	XP_001112405.1
Pan troglodytes (chimpanzee)	PFAS	XP_511854.3
Bos taurus (cattle)	PFAS	NP_001243493.1
Mus musculus (house mouse)	Pfas	NP_001152991.1
Drosophila melanogaster (fruit fly)	ade2	NP_723146.1
Caenorhabditis elegans (roundworm)	CELE_F10F2.2	NP_497942.2
Homo sapiens (human)	PFAS	NP_036525.1
Canis lupus familiaris (dog)	PFAS	XP_005620078.1
Rattus norvegicus (Norway rat)	Pfas	NP_001099261.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002091	XP_320948.2
Arabidopsis thaliana (thale cress)	PUR4	NP_177566.3
Danio rerio (zebrafish)	pfas	NP_001038667.1
Oryza sativa (rice)	Os01g0888500	NP_001045039.1
Xenopus tropicalis (tropical clawed frog)	pfas	NP_001135692.2

Gene Ontology
Bibliography

Related articles in PubMed

Expression of the purine biosynthetic enzyme phosphoribosyl formylglycinamidine synthase (FGAMS) in neurons.
Mangold CA, Yao PJ, Du M, Freeman WM, Benkovic SJ, Szpara ML
Journal of neurochemistry(2018 Jan)

Discovery and characterization of spontaneous mouse models of craniofacial dysmorphology.
Palmer K, Fairfield H, Borgeia S, Curtain M, Hassan MG, Dionne L, Yong Karst S, Coombs H, Bronson RT, Reinholdt LG, Bergstrom DE, Donahue LR, Cox TC, Murray SA
Developmental biology415(2)216-227(2016 07)

Exome sequencing reveals pathogenic mutations in 91 strains of mice with Mendelian disorders.
Fairfield H, Srivastava A, Ananda G, Liu R, Kircher M, Lakshminarayana A, Harris BS, Karst SY, Dionne LA, Kane CC, Curtain M, Berry ML, Ward-Bailey PF, Greenstein I, Byers C, Czechanski A, Sharp J, Palmer K, Gudis P, Martin W, Tadenev A, Bogdanik L, Pratt CH, Chang B, Schroeder DG, Cox GA, Cliften P, Milbrandt J, Murray S, Burgess R, Bergstrom DE, Donahue LR, Hamamy H, Masri A, Santoni FA, Makrythanasis P, Antonarakis SE, Shendure J, Reinholdt LG
Genome research25(7)948-57(2015 Jul)

Mutation discovery in mice by whole exome sequencing.
Fairfield H, Gilbert GJ, Barter M, Corrigan RR, Curtain M, Ding Y, D'Ascenzo M, Gerhardt DJ, He C, Huang W, Richmond T, Rowe L, Probst FJ, Bergstrom DE, Murray SA, Bult C, Richardson J, Kile BT, Gut I, Hager J, Sigurdsson S, Mauceli E, Di Palma F, Lindblad-Toh K, Cunningham ML, Cox TC, Justice MJ, Spector MS, Lowe SW, Albert T, Donahue LR, Jeddeloh J, Shendure J, Reinholdt LG
Genome biology12(9)R86(2011 Sep)

Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.
Okazaki N, F-Kikuno R, Ohara R, Inamoto S, Koseki H, Hiraoka S, Saga Y, Seino S, Nishimura M, Kaisho T, Hoshino K, Kitamura H, Nagase T, Ohara O, Koga H
DNA research : an international journal for rapid publication of reports on genes and genomes11(3)205-18(2004 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Pfas gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pfas cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu16391	NM_001159519.1 Latest version!	Mus musculus phosphoribosylformylglycinamidine synthase (FGAR amidotransferase) (Pfas), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$790.30 $1129.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pfas ( NM_001159519.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001152991.1 Mus musculus phosphoribosylformylglycinamidine synthase (FGAR amidotransferase) (Pfas), mRNA.

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CloneID	OMu16391
Clone ID Related Accession (Same CDS sequence)	NM_001159519.1
Accession Version	NM_001159519.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4014bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	phosphoribosylformylglycinamidine synthase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL645902.6 and AL714000.10. On or before Apr 19, 2009 this sequence version replaced XM_908501.3, XM_111232.8. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGCCCCTG TCCTTCACTT TTATGTCCGC CCCTCTGGCC ATGAGGGGGC AGCATCTGGA 
CGTGTTTTTC GGAGACTACA AGAGAAACTG CCAACACTTC AGAGTGTGGA GACTGAGCTG 
TGCTACAATG TACACTGGGC AGCTGAGACC CTTCCATGGG CTGAGGAGAT GAAGAAGTTG 
ATGTGGCTGT TCGGCTGCCC TTTGGTGCGG GATGATGTTG CTCAGGAGCC CTGGCTTGTT 
CCTGGCTCCA ACGATTTGTT GCTGGAGGTG GGACCCAGGC TGAATTTCTC GACTCCAGCA 
TCGACCAACA TTGTTTCTGT GTGCCAGGCA GCCGGGCTGA GGGCTGTGGA TCGGGTAGAG 
ACCACCCGAC GTTACCGACT TTCGTTTACC GACCACCCTA CAGCTGAAAT GGAAGCCATC 
TCTCTGGCCG CCTTGCACGA CCGGATGACA GAGCAGCACT ACCCTGACCC CATCCAGAGC 
TTCTCTCCTC AGAGCATTCC AGCCCCACTC AAGGGCTCCA TCGACATACT AGCTGAGGGT 
CGGCCTGCCC TGGAGAAGGC CAACCAGGAG CTAGGTTTGG CTCTTGACTC CTGGGACCTC 
GATTTCTATA CCAAGCGCTT TCAAGAGCTG CAGCGGAACC CCAGTACAGT GGAGGTCTTT 
GACTTGGCTC AGTCCAATAG TGAGCACAGC CGACATTGGT TCTTCAAGGG CCAGCTCCAT 
GTGGATGGGA AGAAGCTAGC GCATTCCCTC TTTGAATCCA TCATGAGCAC TCAGGCATCC 
TCCAACCCTA ACAATGTCCT CAAGTTCTGT GACAACAGCA GTGCAATCCA GGGGAAGAAA 
GTCAAATTCC TGCGGCCGGA GGACTCTACC CGCCCAAGCT GCTTCCAGCA ACAGCAGGGG 
CTTAGACATG TTGTCTTCAC AGCAGAGACA CATAACTTCC CCACAGGAGT GGCCCCCTTC 
AGCGGTGCAA CCACAGGCAC GGGTGGCCGC ATCAGAGACG TCCAGTGCAC AGGCCGTGGG 
GCCCACGTGG TAGCTGGCAC TGCTGGCTAT TGCTTTGGAA ACCTGCACAT CCCAGATTAT 
AATCTGCCCT GGGAGGACCC AAGCTTCCAG TATCCTGGGA ACTTTGCCAG GCCTTTGGAG 
GTTGCTATTG AGGCCAGCAA TGGAGCATCT GACTATGGCA ACAAGTTTGG AGAGCCAGTG 
CTGGCTGGAT TTGCCCGCTC TTTGGGTCTC CAGCTTCCAG ATGGTCAGAG ACGTGAGTGG 
ATCAAGCCCA TCATGTTCAG TGGCGGCATT GGGTCCATGG AAGCCAAACA TGTAGGCAAA 
AAGCCCCCAG AGCCAGGCAT GGAGGTTGTA AAGGTTGGAG GTCCTGTTTA TAGGATTGGA 
GTTGGGGGTG GAGCTGCTTC GTCTGTGCAG GTCCAGGGAG ACAACACCAG TGACCTGGAC 
TTTGGAGCTG TGCAACGGGG AGACCCGGAA ATGGAGCAAA AGATGAATCG TGTGATCCGG 
GCTTGTGTGG AGGCCCCAGG AGGAAACCCC ATCTGCAGCC TCCATGACCA GGGTGCTGGT 
GGCAATGGCA ATGTCCTAAA GGAGCTGAGT GACCCAGAAG GAGCCATCAT TTACACCAGC 
CGCTTCCAGC TAGGTGACCC AACCTTGAAT GCACTGGAAA TCTGGGGGGC TGAGTACCAG 
GAGTCAAATG CACTACTGCT TAGGCCCTCT GACCGGGACT TCCTGAGTCG TGCCAGTGCC 
CGGGAACGCT GCCCAGCTTG TTTTGTGGGC ACAATCACTG GAGACAAGAG AATTGTACTA 
GTGGATGATC GTGAATGCCT AGTAGGAAAA ACTGGTCAGG GAGATGCACC CCTGACACCT 
CCAACCCCTG TAGACCTGGA CCTTGACTGG GTGCTGGGAA AGATGCCTCA GAAGGAGTTC 
TTCCTCCAGA GGAAACCCCC TGTGCTGCAG CCTTTGGCTT TGCCCCCAGA ACTGAGTGTG 
CGACAGGCTC TGAACCGGGT CCTAAGGCTG CCTGCTGTGG CCAGCAAACG CTACCTCACC 
AACAAGGTGG ATCGCTCTGT GGGTGGCCTT GTTGCCCAAC AGCAGTGTGT CGGACCCCTG 
CAGACTCCTC TGGCTGATGT GGCTGTTGTA GCACTGAGCC ACCAGGAGTG CATAGGGGCT 
GCCACAGCCC TGGGAGAGCA GCCAGTCAAG AGCCTGCTGG ACCCCAAGGC TGCTGCCAGG 
CTTGCTGTGT CTGAGGCCCT CACCAACCTG GTGTTTGCTC TGGTCACCGA CCTCCGAGAT 
GTGAAGTGCA GTGGGAACTG GATGTGGGCA GCCAAGCTCC CAGGTGAGGG TGCAGCGCTG 
GCTGATGCCT GTGAGGCTAT GGTGGCAGTG ATGGCAGCAC TGGGTGTGGC AGTAGATGGT 
GGCAAAGACT CCCTCAGCAT GGCTGCCCGG GTCGGCACTG AGACGGTGCA GGCTCCTGGG 
TCACTGGTCA TTTCAGCATA TGCTGTCTGT CCAGACATCA CAGCCACTGT GACCCCAGAC 
CTCAAACATC CTGGTGGGAA AGGTCATTTG CTTTATGTGC CTTTGAGCCC CGGGCAGCAC 
CGGCTGGGAG GAACGGCTCT AGCCCAGTGT TTCTCCCAGC TTGGGGAGCA CCCTCCTGAT 
CTAGACCTTC CCGAGAACCT TGTGCGTGCC TTTCATATCA CCCAGGGGCT GCTGAAAGAA 
TGCCGTCTTT GCTCAGGCCA TGATGTCAGC GATGGGGGGC TTGTCACCTG CCTTCTAGAG 
ATGGCCTTTG CTGGGAACTG TGGGATAGAG GTAGATGTAC CTGCCCCTGG GATCCATGCA 
TTGCCTGTGC TGTTTGCGGA AGAGCCCGGT CTGGTGTTGG AGGTACAGGA GGCAGATGTG 
GCCGGAGTAC GGCAGAGGTA CGAGAGTGCT GGCCTACGGT GTCTGGAACT GGGCCACACA 
GGCGAGGCTG GACCCCAGGC CATGGCCCGA ATATCAGTGA ATAAGGCTGT GGTAGTAGAG 
GAGCCTGTTG GGGAGTTAAG AGCCCTCTGG GAGGAAACAA GTTTCCAGCT GGACCTACTG 
CAGGCAGAGC CACGCTGTGT GATAGAGGAG AAACAGGGCC TGAAGGAGCG GACAGGGCCC 
AGCTACTACC TGCCGCCCAC CTTTCCTGTG GCCTCTGTGC CCTGTAAACC TGGTGGACCC 
GTCCCTCGAG TTGCCATCTT GCGAGAAGAG GGCAGCAATG GAGACCGAGA AATGGCTGAT 
GCCTTTCACT TGGCAGGCTT TGAGGTGTGG GATGTGACCA TGCAAGATCT CTGCTCTGGG 
GCCATCAGGT TGGACACATT CCGTGGTGTG GCCTTTGTGG GCGGCTTCAG CTACGCAGAC 
GTCCTGGGCT CTGCTAAAGG TTGGGCAGCT GCGGTGACCT TTAATCCACA GGCCAGGGAA 
GAGCTGGGCC GATTCCGAAG GCGGCCGGAC ACCTTCAGCC TGGGCGTGTG TAATGGCTGT 
CAGCTGCTGG CTCTTCTGGG CTGGGTGGGA AGTGATCCCA GTGAAGAGCA AGCTGAGCCA 
GGCCAGGACT CCCAGCCAAC ACAGCCGGGC CTCCTGCTCC GCCACAATCT GTCTGGGCGG 
TTCGAGTCCC GATGGGCCAC CGTACGTGTG GAGCCGGGGC CAGCCCTGAT GCTCCGAGGG 
ATGGAAGGCT CTGTGCTGCC CGTGTGGAGT GCTCACGGGG AAGGCTACAT GGCATTTTCT 
TCTCCTGAAC TCCAAGCCAA GATCGAGGCC AAGGGCCTGG TTCCTCTGCA CTGGGCAGAC 
GATGATGGGA ACCCCACAGA GCAGTACCCT CTGAACCCCA ATGGATCTCC AGGGGGCATA 
GCTGGTATCT GTTCCCAAGA TGGCCGACAC CTGGCACTCA TGCCGCACCC TGAGCGGGCT 
GTAAGGCTTT GGCAGTGGGC ATGGCGACCC TCTCCCTTTG ATGTTCTTCC CACCTCCCCG 
TGGCTGCAGC TCTTCATCAA TGCTAGGAAC TGGACCCAGG AGGACAGCTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001152991.1
CDS	94..4107
Translation	MAPVLHFYVRPSGHEGAASGRVFRRLQEKLPTLQSVETELCYNVHWAAETLPWAEEMKKLMWLFGCPLVRDDVAQEPWLVPGSNDLLLEVGPRLNFSTPASTNIVSVCQAAGLRAVDRVETTRRYRLSFTDHPTAEMEAISLAALHDRMTEQHYPDPIQSFSPQSIPAPLKGSIDILAEGRPALEKANQELGLALDSWDLDFYTKRFQELQRNPSTVEVFDLAQSNSEHSRHWFFKGQLHVDGKKLAHSLFESIMSTQASSNPNNVLKFCDNSSAIQGKKVKFLRPEDSTRPSCFQQQQGLRHVVFTAETHNFPTGVAPFSGATTGTGGRIRDVQCTGRGAHVVAGTAGYCFGNLHIPDYNLPWEDPSFQYPGNFARPLEVAIEASNGASDYGNKFGEPVLAGFARSLGLQLPDGQRREWIKPIMFSGGIGSMEAKHVGKKPPEPGMEVVKVGGPVYRIGVGGGAASSVQVQGDNTSDLDFGAVQRGDPEMEQKMNRVIRACVEAPGGNPICSLHDQGAGGNGNVLKELSDPEGAIIYTSRFQLGDPTLNALEIWGAEYQESNALLLRPSDRDFLSRASARERCPACFVGTITGDKRIVLVDDRECLVGKTGQGDAPLTPPTPVDLDLDWVLGKMPQKEFFLQRKPPVLQPLALPPELSVRQALNRVLRLPAVASKRYLTNKVDRSVGGLVAQQQCVGPLQTPLADVAVVALSHQECIGAATALGEQPVKSLLDPKAAARLAVSEALTNLVFALVTDLRDVKCSGNWMWAAKLPGEGAALADACEAMVAVMAALGVAVDGGKDSLSMAARVGTETVQAPGSLVISAYAVCPDITATVTPDLKHPGGKGHLLYVPLSPGQHRLGGTALAQCFSQLGEHPPDLDLPENLVRAFHITQGLLKECRLCSGHDVSDGGLVTCLLEMAFAGNCGIEVDVPAPGIHALPVLFAEEPGLVLEVQEADVAGVRQRYESAGLRCLELGHTGEAGPQAMARISVNKAVVVEEPVGELRALWEETSFQLDLLQAEPRCVIEEKQGLKERTGPSYYLPPTFPVASVPCKPGGPVPRVAILREEGSNGDREMADAFHLAGFEVWDVTMQDLCSGAIRLDTFRGVAFVGGFSYADVLGSAKGWAAAVTFNPQAREELGRFRRRPDTFSLGVCNGCQLLALLGWVGSDPSEEQAEPGQDSQPTQPGLLLRHNLSGRFESRWATVRVEPGPALMLRGMEGSVLPVWSAHGEGYMAFSSPELQAKIEAKGLVPLHWADDDGNPTEQYPLNPNGSPGGIAGICSQDGRHLALMPHPERAVRLWQWAWRPSPFDVLPTSPWLQLFINARNWTQEDSC

Target ORF information:

RefSeq Version	NM_001159519.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphoribosylformylglycinamidine synthase (FGAR amidotransferase) (Pfas), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001159519.1

ORF Insert Sequence:

ATGGCCCCTG TCCTTCACTT TTATGTCCGC CCCTCTGGCC ATGAGGGGGC AGCATCTGGA 
CGTGTTTTTC GGAGACTACA AGAGAAACTG CCAACACTTC AGAGTGTGGA GACTGAGCTG 
TGCTACAATG TACACTGGGC AGCTGAGACC CTTCCATGGG CTGAGGAGAT GAAGAAGTTG 
ATGTGGCTGT TCGGCTGCCC TTTGGTGCGG GATGATGTTG CTCAGGAGCC CTGGCTTGTT 
CCTGGCTCCA ACGATTTGTT GCTGGAGGTG GGACCCAGGC TGAATTTCTC GACTCCAGCA 
TCGACCAACA TTGTTTCTGT GTGCCAGGCA GCCGGGCTGA GGGCTGTGGA TCGGGTAGAG 
ACCACCCGAC GTTACCGACT TTCGTTTACC GACCACCCTA CAGCTGAAAT GGAAGCCATC 
TCTCTGGCCG CCTTGCACGA CCGGATGACA GAGCAGCACT ACCCTGACCC CATCCAGAGC 
TTCTCTCCTC AGAGCATTCC AGCCCCACTC AAGGGCTCCA TCGACATACT AGCTGAGGGT 
CGGCCTGCCC TGGAGAAGGC CAACCAGGAG CTAGGTTTGG CTCTTGACTC CTGGGACCTC 
GATTTCTATA CCAAGCGCTT TCAAGAGCTG CAGCGGAACC CCAGTACAGT GGAGGTCTTT 
GACTTGGCTC AGTCCAATAG TGAGCACAGC CGACATTGGT TCTTCAAGGG CCAGCTCCAT 
GTGGATGGGA AGAAGCTAGC GCATTCCCTC TTTGAATCCA TCATGAGCAC TCAGGCATCC 
TCCAACCCTA ACAATGTCCT CAAGTTCTGT GACAACAGCA GTGCAATCCA GGGGAAGAAA 
GTCAAATTCC TGCGGCCGGA GGACTCTACC CGCCCAAGCT GCTTCCAGCA ACAGCAGGGG 
CTTAGACATG TTGTCTTCAC AGCAGAGACA CATAACTTCC CCACAGGAGT GGCCCCCTTC 
AGCGGTGCAA CCACAGGCAC GGGTGGCCGC ATCAGAGACG TCCAGTGCAC AGGCCGTGGG 
GCCCACGTGG TAGCTGGCAC TGCTGGCTAT TGCTTTGGAA ACCTGCACAT CCCAGATTAT 
AATCTGCCCT GGGAGGACCC AAGCTTCCAG TATCCTGGGA ACTTTGCCAG GCCTTTGGAG 
GTTGCTATTG AGGCCAGCAA TGGAGCATCT GACTATGGCA ACAAGTTTGG AGAGCCAGTG 
CTGGCTGGAT TTGCCCGCTC TTTGGGTCTC CAGCTTCCAG ATGGTCAGAG ACGTGAGTGG 
ATCAAGCCCA TCATGTTCAG TGGCGGCATT GGGTCCATGG AAGCCAAACA TGTAGGCAAA 
AAGCCCCCAG AGCCAGGCAT GGAGGTTGTA AAGGTTGGAG GTCCTGTTTA TAGGATTGGA 
GTTGGGGGTG GAGCTGCTTC GTCTGTGCAG GTCCAGGGAG ACAACACCAG TGACCTGGAC 
TTTGGAGCTG TGCAACGGGG AGACCCGGAA ATGGAGCAAA AGATGAATCG TGTGATCCGG 
GCTTGTGTGG AGGCCCCAGG AGGAAACCCC ATCTGCAGCC TCCATGACCA GGGTGCTGGT 
GGCAATGGCA ATGTCCTAAA GGAGCTGAGT GACCCAGAAG GAGCCATCAT TTACACCAGC 
CGCTTCCAGC TAGGTGACCC AACCTTGAAT GCACTGGAAA TCTGGGGGGC TGAGTACCAG 
GAGTCAAATG CACTACTGCT TAGGCCCTCT GACCGGGACT TCCTGAGTCG TGCCAGTGCC 
CGGGAACGCT GCCCAGCTTG TTTTGTGGGC ACAATCACTG GAGACAAGAG AATTGTACTA 
GTGGATGATC GTGAATGCCT AGTAGGAAAA ACTGGTCAGG GAGATGCACC CCTGACACCT 
CCAACCCCTG TAGACCTGGA CCTTGACTGG GTGCTGGGAA AGATGCCTCA GAAGGAGTTC 
TTCCTCCAGA GGAAACCCCC TGTGCTGCAG CCTTTGGCTT TGCCCCCAGA ACTGAGTGTG 
CGACAGGCTC TGAACCGGGT CCTAAGGCTG CCTGCTGTGG CCAGCAAACG CTACCTCACC 
AACAAGGTGG ATCGCTCTGT GGGTGGCCTT GTTGCCCAAC AGCAGTGTGT CGGACCCCTG 
CAGACTCCTC TGGCTGATGT GGCTGTTGTA GCACTGAGCC ACCAGGAGTG CATAGGGGCT 
GCCACAGCCC TGGGAGAGCA GCCAGTCAAG AGCCTGCTGG ACCCCAAGGC TGCTGCCAGG 
CTTGCTGTGT CTGAGGCCCT CACCAACCTG GTGTTTGCTC TGGTCACCGA CCTCCGAGAT 
GTGAAGTGCA GTGGGAACTG GATGTGGGCA GCCAAGCTCC CAGGTGAGGG TGCAGCGCTG 
GCTGATGCCT GTGAGGCTAT GGTGGCAGTG ATGGCAGCAC TGGGTGTGGC AGTAGATGGT 
GGCAAAGACT CCCTCAGCAT GGCTGCCCGG GTCGGCACTG AGACGGTGCA GGCTCCTGGG 
TCACTGGTCA TTTCAGCATA TGCTGTCTGT CCAGACATCA CAGCCACTGT GACCCCAGAC 
CTCAAACATC CTGGTGGGAA AGGTCATTTG CTTTATGTGC CTTTGAGCCC CGGGCAGCAC 
CGGCTGGGAG GAACGGCTCT AGCCCAGTGT TTCTCCCAGC TTGGGGAGCA CCCTCCTGAT 
CTAGACCTTC CCGAGAACCT TGTGCGTGCC TTTCATATCA CCCAGGGGCT GCTGAAAGAA 
TGCCGTCTTT GCTCAGGCCA TGATGTCAGC GATGGGGGGC TTGTCACCTG CCTTCTAGAG 
ATGGCCTTTG CTGGGAACTG TGGGATAGAG GTAGATGTAC CTGCCCCTGG GATCCATGCA 
TTGCCTGTGC TGTTTGCGGA AGAGCCCGGT CTGGTGTTGG AGGTACAGGA GGCAGATGTG 
GCCGGAGTAC GGCAGAGGTA CGAGAGTGCT GGCCTACGGT GTCTGGAACT GGGCCACACA 
GGCGAGGCTG GACCCCAGGC CATGGCCCGA ATATCAGTGA ATAAGGCTGT GGTAGTAGAG 
GAGCCTGTTG GGGAGTTAAG AGCCCTCTGG GAGGAAACAA GTTTCCAGCT GGACCTACTG 
CAGGCAGAGC CACGCTGTGT GATAGAGGAG AAACAGGGCC TGAAGGAGCG GACAGGGCCC 
AGCTACTACC TGCCGCCCAC CTTTCCTGTG GCCTCTGTGC CCTGTAAACC TGGTGGACCC 
GTCCCTCGAG TTGCCATCTT GCGAGAAGAG GGCAGCAATG GAGACCGAGA AATGGCTGAT 
GCCTTTCACT TGGCAGGCTT TGAGGTGTGG GATGTGACCA TGCAAGATCT CTGCTCTGGG 
GCCATCAGGT TGGACACATT CCGTGGTGTG GCCTTTGTGG GCGGCTTCAG CTACGCAGAC 
GTCCTGGGCT CTGCTAAAGG TTGGGCAGCT GCGGTGACCT TTAATCCACA GGCCAGGGAA 
GAGCTGGGCC GATTCCGAAG GCGGCCGGAC ACCTTCAGCC TGGGCGTGTG TAATGGCTGT 
CAGCTGCTGG CTCTTCTGGG CTGGGTGGGA AGTGATCCCA GTGAAGAGCA AGCTGAGCCA 
GGCCAGGACT CCCAGCCAAC ACAGCCGGGC CTCCTGCTCC GCCACAATCT GTCTGGGCGG 
TTCGAGTCCC GATGGGCCAC CGTACGTGTG GAGCCGGGGC CAGCCCTGAT GCTCCGAGGG 
ATGGAAGGCT CTGTGCTGCC CGTGTGGAGT GCTCACGGGG AAGGCTACAT GGCATTTTCT 
TCTCCTGAAC TCCAAGCCAA GATCGAGGCC AAGGGCCTGG TTCCTCTGCA CTGGGCAGAC 
GATGATGGGA ACCCCACAGA GCAGTACCCT CTGAACCCCA ATGGATCTCC AGGGGGCATA 
GCTGGTATCT GTTCCCAAGA TGGCCGACAC CTGGCACTCA TGCCGCACCC TGAGCGGGCT 
GTAAGGCTTT GGCAGTGGGC ATGGCGACCC TCTCCCTTTG ATGTTCTTCC CACCTCCCCG 
TGGCTGCAGC TCTTCATCAA TGCTAGGAAC TGGACCCAGG AGGACAGCTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Expression of the purine biosynthetic enzyme phosphoribosyl formylglycinamidine synthase (FGAMS) in neurons.
Journal of neurochemistry(2018 Jan)
Mangold CA,Yao PJ,Du M,Freeman WM,Benkovic SJ,Szpara ML

Discovery and characterization of spontaneous mouse models of craniofacial dysmorphology.
Developmental biology415(2)216-227(2016 07)
Palmer K,Fairfield H,Borgeia S,Curtain M,Hassan MG,Dionne L,Yong Karst S,Coombs H,Bronson RT,Reinholdt LG,Bergstrom DE,Donahue LR,Cox TC,Murray SA

Exome sequencing reveals pathogenic mutations in 91 strains of mice with Mendelian disorders.
Genome research25(7)948-57(2015 Jul)
Fairfield H,Srivastava A,Ananda G,Liu R,Kircher M,Lakshminarayana A,Harris BS,Karst SY,Dionne LA,Kane CC,Curtain M,Berry ML,Ward-Bailey PF,Greenstein I,Byers C,Czechanski A,Sharp J,Palmer K,Gudis P,Martin W,Tadenev A,Bogdanik L,Pratt CH,Chang B,Schroeder DG,Cox GA,Cliften P,Milbrandt J,Murray S,Burgess R,Bergstrom DE,Donahue LR,Hamamy H,Masri A,Santoni FA,Makrythanasis P,Antonarakis SE,Shendure J,Reinholdt LG

Mutation discovery in mice by whole exome sequencing.
Genome biology12(9)R86(2011 Sep)
Fairfield H,Gilbert GJ,Barter M,Corrigan RR,Curtain M,Ding Y,D'Ascenzo M,Gerhardt DJ,He C,Huang W,Richmond T,Rowe L,Probst FJ,Bergstrom DE,Murray SA,Bult C,Richardson J,Kile BT,Gut I,Hager J,Sigurdsson S,Mauceli E,Di Palma F,Lindblad-Toh K,Cunningham ML,Cox TC,Justice MJ,Spector MS,Lowe SW,Albert T,Donahue LR,Jeddeloh J,Shendure J,Reinholdt LG

Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.
DNA research : an international journal for rapid publication of reports on genes and genomes11(3)205-18(2004 Jun)
Okazaki N,F-Kikuno R,Ohara R,Inamoto S,Koseki H,Hiraoka S,Saga Y,Seino S,Nishimura M,Kaisho T,Hoshino K,Kitamura H,Nagase T,Ohara O,Koga H

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Pfas cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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