PFAS cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	PFAS
Entrez Gene ID	520318
Full Name	phosphoribosylformylglycinamidine synthase
General protein information	Preferred Names phosphoribosylformylglycinamidine synthase Names phosphoribosylformylglycinamidine synthase FGAR amidotransferase
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024980167.1	XP_024835935.1	phosphoribosylformylglycinamidine synthase isoform X1
XM_005220359.4	XP_005220416.4	phosphoribosylformylglycinamidine synthase isoform X3
XM_010816030.2	XP_010814332.1	phosphoribosylformylglycinamidine synthase isoform X3
XM_015458726.1	XP_015314212.1	phosphoribosylformylglycinamidine synthase isoform X1
XM_024980168.1	XP_024835936.1	phosphoribosylformylglycinamidine synthase isoform X1
XM_015458726.2	XP_015314212.2	phosphoribosylformylglycinamidine synthase isoform X2
XM_005220359.3	XP_005220416.3	phosphoribosylformylglycinamidine synthase isoform X2
NM_001256564.1	NP_001243493.1	phosphoribosylformylglycinamidine synthase

Pathways from BioSystems

Homologies

	PFAS	XP_001112405.1
Pan troglodytes (chimpanzee)	PFAS	XP_511854.3
Bos taurus (cattle)	PFAS	NP_001243493.1
Mus musculus (house mouse)	Pfas	NP_001152991.1
Drosophila melanogaster (fruit fly)	ade2	NP_723146.1
Caenorhabditis elegans (roundworm)	CELE_F10F2.2	NP_497942.2
Homo sapiens (human)	PFAS	NP_036525.1
Canis lupus familiaris (dog)	PFAS	XP_005620078.1
Rattus norvegicus (Norway rat)	Pfas	NP_001099261.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002091	XP_320948.2
Arabidopsis thaliana (thale cress)	PUR4	NP_177566.3
Danio rerio (zebrafish)	pfas	NP_001038667.1
Oryza sativa (rice)	Os01g0888500	NP_001045039.1
Xenopus tropicalis (tropical clawed frog)	pfas	NP_001135692.2

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Mapping of the bovine genes of the de novo AMP synthesis pathway.
B?nsdorff T, Gautier M, Farstad W, R?nningen K, Lingaas F, Olsaker I
Animal genetics35(6)438-44(2004 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PFAS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PFAS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa226375	XM_024980168.1 Latest version!	Bos taurus phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa226375	XM_024980167.1 Latest version!	Bos taurus phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa226376	XM_015458726.2 Latest version!	Bos taurus phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa226377	XM_005220359.4 Latest version!	Bos taurus phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa93869	XM_015458726.1 Latest version!	Bos taurus phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBa93870	XM_005220359.3 Latest version!	Bos taurus phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBa29748	NM_001256564.1 Latest version!	Bos taurus phosphoribosylformylglycinamidine synthase (PFAS), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa29748	XM_010816030.2 Latest version!	Bos taurus phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PFAS ( XM_024980167.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024835935.1 Bos taurus phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa226375
Clone ID Related Accession (Same CDS sequence)	XM_024980167.1 , XM_024980168.1
Accession Version	XM_024980167.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	phosphoribosylformylglycinamidine synthase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTCCG TCCTTCACTT CTATGTCCGC CCCTCTGGCC ACGAGAGGGC AGCCTCTGAA 
TACATTCAAA GGAAGCTGCA AAGGGAACTG CCAGAGCTGC AGGGTGTCAA GACCGAGCAG 
TGCTACAATG TGAACTGGAC AGCGGAGTCC TTCCCGAGCA ACAAGGAGAT GAAGAAGCTG 
ACATGGCTAT TTGGCTGCCC CTTGTTACTG GACGATGTTG CTCAGGAATC CTGGCTTCGT 
CCTGGCCCCA CAGACCTGCT GCTGGAGGTC GGACCCAGGC TGAACTTTTC CACCCCGACA 
TCCAGCAACA TTGTGTCAGT GTGCCAGGCT GCAGGGCTGG GGGCTGTGGA CCGCGTGGAG 
CCAACCCGGC GCTACCTGCT CTCGTTTGCC CACCCACCTT CAGCTGACCT GGAGGCCATT 
GCCCTGGCTA CCCTGCATGA CCGGATGACA GAGCAGCGCT TCCCCCAGCC CATCCAGAGC 
TTCTCTTCAG GGTGCATCCC AGCACCCCTG AGCGGCCCAA TTGACGTGCT GGCTGAGGGC 
CGGTCTGCCC TGGAGAAAGC CAACCAGGAG CTAGGTCTGG CCCTAGACTC CTGGGACCTG 
GATTTCTACA CCAAGCGCTT CCAGGAGCTG CAGCGCAACC CCAGCACTGT GGAAGTCTTC 
GACTTGGCTC AGTCCAATAG CGAACACAGC CGACACTGGT TCTTCAAGGG TCGACTCCAT 
GTGGATGGAC AGGAGCTGCC ACACTCGCTG TTTGAGTCCA TCATGAGCAC CCAGGCGTCC 
TCCAACCCTA ACAACGTCCT CAAGTTCTGT GACAACAGCA GTGCCATCCA GGGGAAAGAA 
GTCCGATTCT TGCGGCCTAA GGACCCCACA CGGCCAAGCT CCTTCCAGCA ATGTCAGGGG 
CTGAGACATG TTGTCTTCAC AGCAGAGACA CACAACTTCC CTACAGGAGT AGCCCCCTTC 
AGTGGTGCAA CCACTGGCAC AGGGGGCCGG ATCCGAGATG TCCAGTGCAC AGGCCGAGGG 
GCCCACGTGG TGGCTGGCAC TGCTGGCTAT TGCTTTGGAA ACCTGCACAT CCCAGGTTAC 
AGTATGCCCT GGGAGGATCC AAGCTTCCAG TATCCAGGAA ACTTTGCCCG ACCCCTGGAG 
ATTGCCATTG AGGCCAGTAA TGGGGCTTCT GACTACGGCA ACAAGTTTGG GGAACCAGTG 
CTTGCTGGCT TTGCCCGCTC CTTGGGCCTT CAGCTCCCGG ACGGCCAGCG GCGTGAGTGG 
ATCAAGCCCA TCATGTTTAG TGGAGGCATT GGGTCCATGG AAGCTGATCA TGTGAGCAAG 
GAGCCCCCAG AGACAGGCAT GGATGTCGTG AAGATTGGCG GTCCTGTCTA CAGGATTGGA 
GTTGGGGGCG GAGCTGCTTC GTCTGTGCAG GTGCAAGGAG ACAACGCCAG TGACCTGGAT 
TTTGGGGCTG TGCAGCGGGG AGACCCAGAA ATGGAGCAGA AGATGAACCG TGTGATCCGG 
GCTTGTGTGG AGGCCCCTGG GGGAAACCCC ATCTGCAGCC TCCATGACCA GGGTGCTGGT 
GGCAATGGCA ATGTCCTGAA GGAGCTGAGT GACCCGGCTG GAGCCATCAT TCACACCAGC 
TGCTTCCAGC TGGGGGACCC AACCCTGAAT GCCCTGGAAA TCTGGGGGGC CGAGTACCAG 
GAGTCGAACG CACTTCTGCT GAGGCCCTCT GACCGGGACT TCCTGAGTTG TGTCAGCGCC 
CGGGAACGCT GCCCAGTCTG CTTTGTGGGC ACCATCACTG GAGACAGGAG AATAGTGCTG 
GTGGATGATC GGGAGTGGCC TATGGGCACA GGTGGCCAGG TGGATGCGCC CCCCACTCCT 
CCCCCAACCC CTGTGGACCT GGACCTGGAC TGGGTGCTGG GCAAGATGCC CCAGAAGGAG 
TTCTTCCTCC AGAGGAGTCT CCCCTTGCTG CAGCCTCTGA CTCTGCCCCC AGGGCTGAGC 
GTGCGCCAGG CTCTGGCGCG GGTCCTCAGG CTGCCTGCCG TGGCCAGCAA GCGCTACCTC 
ACCAACAAGG TGGACCGTTC TGTGGGCGGC CTGGTGGCCC AGCAGCAGTG TGTGGGACCC 
CTGCAGACCC CTCTGGCAGA TGTGGCGGTT GTGGCACTGA GTCACCAGGA GCTTGTAGGG 
GCCGCCACAG CCCTGGGAGA GCAGCCAGTC AAGAGCCTGC TGGACCCGAA GGTTGCCGCC 
CGGCTGGCTG TGGCCGAAGC CCTCACCAAC CTGGTATTTG CTCTGGTCAC CGACCTCCAG 
GATGTGAAAT GCAGCGGGAA CTGGATGTGG GCAGCCAAGC TCCCAGGGGA GGGTGCAGCT 
CTGGCTGACG CCTGTGAGGC TATGGTGGCA GTGATGGCGG CCCTGGGTGT AGCGGTAGAC 
GGTGGCAAGG ACTCCCTCAG CATGGCTGCC CGGGTTGGCT CTGAAACCGT GCGGGCTCCT 
GGGTCACTGG TCATCTCAGC CTATGCTGTC TGTTCAGACA TTACAGCCAC TGTGACCCCA 
GACCTCAAGC GTCCTGGGGG GAGAGGCCAC CTGCTCTACG TGCCTCTGAG TCCTGGGCAG 
CACCGGCTGG GGGGCACAGC TCTGGCTCAG TGCTTCTCCC AGCTTGGCGA GCAGCCTCCC 
GACCTGGATG TTCCTGAGAA CTTGGTGCGA GCCTTCAGCA TCACCCAGGG GCTGCTGAAA 
GACCGCCTCC TCTGCTCAGG CCATGATGTC AGTGATGGAG GTCTCATCAC TTGCCTGCTA 
GAGATGGCCT TTGCTGGGAA TTGTGGGATA GAGGTGGACA TCCCCGCACC TGGGGTCGAT 
GCCCTGCCTG TGCTGTTCGC CGAGGAGCCA GGCCTGGTGC TGGAGGTGCA GGAGCCGGAC 
CTCGCCCAAG TGCTGATGCG TTACCGGAAT GCTGGCCTCC ACTGCCTAGA GCTGGGTCTC 
ACAGGCGACA CGGGGCCCCA CGCCATGGTC CGCGTGTCGG TGAACAGGAC GGTGGTTCTG 
GAGGAACCTG TTGGGCAGCT ACGAGCCCTC TGGGAGGAAA CCAGTTTCCA GTTGGACCGG 
CTACAGGCAA AACCACACTG TGTGGCCCAG GAAGAGCAAG GGCTGAGGGA GCGGGCAGGG 
CCCACCTTCT GCCTGCCGCC TACCTTCCCC AGAGCTTCCG TGCCTTGTGA GCCTGGTGGT 
CCTGCCCCCC GAGTCGCCAT CATTCGGGAA GAGGGCAGTA ATGGAGACCG GGAGATGGCA 
GATGCCTTCC ACTTGGCTGG ATTTGAGGTG TGGGATGTAA CCATGCAGGA CCTGTGCTCT 
GGAGCCATCG GGCTGGACAC ATTCCGCGGT GTGGCCTTCG TAGGCGGCTT CAGCTACGCG 
GACGTCCTGG GCTCTGCCAA AGGCTGGGCA GCTGCTGTGA CCTTTAACCC GCAGGCCGGG 
GCTGAGATGA AGCGCTTCCG CCAGCGGCCA GACACCTTCA GCCTGGGCGT GTGTAACGGC 
TGTCAGCTGC TGGCCCTGCT GGGCTGGGTG GGAGGCAGTT CTAGTGAGGA GGTGGTGGAG 
ATGTGCCAGG AGTCCTGGCC GGCCCGGCCC GGTCTCCTGC TACGCCACAA CCTGTCTGGG 
CGCTTTGAGT CGCGCTGGGC TAGTGTGCGC GTGGGGCCCG GGCCAGCCCT GATGCTGCGA 
GGGATGGAAG GCGCGGTGCT GCCCGTGTGG AGCGCTCACG GGGAAGGTTA CATGGCATTT 
TCTTCTCCGG AACTCCAAGC CCAGATGGAG GCCAGGGGCT TAGCTCCACT GCACTGGGCA 
GATGATGATG GGAACCCCAC GGAACAGTAC CCCCTGAATC CCAACGGCTC CCCAGGGGGT 
GTCGCTGGCA TCTGCTCCCC CGATGGCCGC CACTTGGCCC TCATGCCTCA CCCGGAGCGG 
TCTGTGAGGC CTTGGCAGTG GGCCTGGCGA CCTCCTCCAT TTGACACTCT AACCACCTCC 
CCCTGGCTCC AGCTCTTCAT CAATGCCCGG AATTGGACCC AGGAATGCAG CTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024835935.1
CDS	80..4096
Translation	MSSVLHFYVRPSGHERAASEYIQRKLQRELPELQGVKTEQCYNVNWTAESFPSNKEMKKLTWLFGCPLLLDDVAQESWLRPGPTDLLLEVGPRLNFSTPTSSNIVSVCQAAGLGAVDRVEPTRRYLLSFAHPPSADLEAIALATLHDRMTEQRFPQPIQSFSSGCIPAPLSGPIDVLAEGRSALEKANQELGLALDSWDLDFYTKRFQELQRNPSTVEVFDLAQSNSEHSRHWFFKGRLHVDGQELPHSLFESIMSTQASSNPNNVLKFCDNSSAIQGKEVRFLRPKDPTRPSSFQQCQGLRHVVFTAETHNFPTGVAPFSGATTGTGGRIRDVQCTGRGAHVVAGTAGYCFGNLHIPGYSMPWEDPSFQYPGNFARPLEIAIEASNGASDYGNKFGEPVLAGFARSLGLQLPDGQRREWIKPIMFSGGIGSMEADHVSKEPPETGMDVVKIGGPVYRIGVGGGAASSVQVQGDNASDLDFGAVQRGDPEMEQKMNRVIRACVEAPGGNPICSLHDQGAGGNGNVLKELSDPAGAIIHTSCFQLGDPTLNALEIWGAEYQESNALLLRPSDRDFLSCVSARERCPVCFVGTITGDRRIVLVDDREWPMGTGGQVDAPPTPPPTPVDLDLDWVLGKMPQKEFFLQRSLPLLQPLTLPPGLSVRQALARVLRLPAVASKRYLTNKVDRSVGGLVAQQQCVGPLQTPLADVAVVALSHQELVGAATALGEQPVKSLLDPKVAARLAVAEALTNLVFALVTDLQDVKCSGNWMWAAKLPGEGAALADACEAMVAVMAALGVAVDGGKDSLSMAARVGSETVRAPGSLVISAYAVCSDITATVTPDLKRPGGRGHLLYVPLSPGQHRLGGTALAQCFSQLGEQPPDLDVPENLVRAFSITQGLLKDRLLCSGHDVSDGGLITCLLEMAFAGNCGIEVDIPAPGVDALPVLFAEEPGLVLEVQEPDLAQVLMRYRNAGLHCLELGLTGDTGPHAMVRVSVNRTVVLEEPVGQLRALWEETSFQLDRLQAKPHCVAQEEQGLRERAGPTFCLPPTFPRASVPCEPGGPAPRVAIIREEGSNGDREMADAFHLAGFEVWDVTMQDLCSGAIGLDTFRGVAFVGGFSYADVLGSAKGWAAAVTFNPQAGAEMKRFRQRPDTFSLGVCNGCQLLALLGWVGGSSSEEVVEMCQESWPARPGLLLRHNLSGRFESRWASVRVGPGPALMLRGMEGAVLPVWSAHGEGYMAFSSPELQAQMEARGLAPLHWADDDGNPTEQYPLNPNGSPGGVAGICSPDGRHLALMPHPERSVRPWQWAWRPPPFDTLTTSPWLQLFINARNWTQECSC

Target ORF information:

RefSeq Version	XM_024980167.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024980167.1

ORF Insert Sequence:

ATGTCCTCCG TCCTTCACTT CTATGTCCGC CCCTCTGGCC ACGAGAGGGC AGCCTCTGAA 
TACATTCAAA GGAAGCTGCA AAGGGAACTG CCAGAGCTGC AGGGTGTCAA GACCGAGCAG 
TGCTACAATG TGAACTGGAC AGCGGAGTCC TTCCCGAGCA ACAAGGAGAT GAAGAAGCTG 
ACATGGCTAT TTGGCTGCCC CTTGTTACTG GACGATGTTG CTCAGGAATC CTGGCTTCGT 
CCTGGCCCCA CAGACCTGCT GCTGGAGGTC GGACCCAGGC TGAACTTTTC CACCCCGACA 
TCCAGCAACA TTGTGTCAGT GTGCCAGGCT GCAGGGCTGG GGGCTGTGGA CCGCGTGGAG 
CCAACCCGGC GCTACCTGCT CTCGTTTGCC CACCCACCTT CAGCTGACCT GGAGGCCATT 
GCCCTGGCTA CCCTGCATGA CCGGATGACA GAGCAGCGCT TCCCCCAGCC CATCCAGAGC 
TTCTCTTCAG GGTGCATCCC AGCACCCCTG AGCGGCCCAA TTGACGTGCT GGCTGAGGGC 
CGGTCTGCCC TGGAGAAAGC CAACCAGGAG CTAGGTCTGG CCCTAGACTC CTGGGACCTG 
GATTTCTACA CCAAGCGCTT CCAGGAGCTG CAGCGCAACC CCAGCACTGT GGAAGTCTTC 
GACTTGGCTC AGTCCAATAG CGAACACAGC CGACACTGGT TCTTCAAGGG TCGACTCCAT 
GTGGATGGAC AGGAGCTGCC ACACTCGCTG TTTGAGTCCA TCATGAGCAC CCAGGCGTCC 
TCCAACCCTA ACAACGTCCT CAAGTTCTGT GACAACAGCA GTGCCATCCA GGGGAAAGAA 
GTCCGATTCT TGCGGCCTAA GGACCCCACA CGGCCAAGCT CCTTCCAGCA ATGTCAGGGG 
CTGAGACATG TTGTCTTCAC AGCAGAGACA CACAACTTCC CTACAGGAGT AGCCCCCTTC 
AGTGGTGCAA CCACTGGCAC AGGGGGCCGG ATCCGAGATG TCCAGTGCAC AGGCCGAGGG 
GCCCACGTGG TGGCTGGCAC TGCTGGCTAT TGCTTTGGAA ACCTGCACAT CCCAGGTTAC 
AGTATGCCCT GGGAGGATCC AAGCTTCCAG TATCCAGGAA ACTTTGCCCG ACCCCTGGAG 
ATTGCCATTG AGGCCAGTAA TGGGGCTTCT GACTACGGCA ACAAGTTTGG GGAACCAGTG 
CTTGCTGGCT TTGCCCGCTC CTTGGGCCTT CAGCTCCCGG ACGGCCAGCG GCGTGAGTGG 
ATCAAGCCCA TCATGTTTAG TGGAGGCATT GGGTCCATGG AAGCTGATCA TGTGAGCAAG 
GAGCCCCCAG AGACAGGCAT GGATGTCGTG AAGATTGGCG GTCCTGTCTA CAGGATTGGA 
GTTGGGGGCG GAGCTGCTTC GTCTGTGCAG GTGCAAGGAG ACAACGCCAG TGACCTGGAT 
TTTGGGGCTG TGCAGCGGGG AGACCCAGAA ATGGAGCAGA AGATGAACCG TGTGATCCGG 
GCTTGTGTGG AGGCCCCTGG GGGAAACCCC ATCTGCAGCC TCCATGACCA GGGTGCTGGT 
GGCAATGGCA ATGTCCTGAA GGAGCTGAGT GACCCGGCTG GAGCCATCAT TCACACCAGC 
TGCTTCCAGC TGGGGGACCC AACCCTGAAT GCCCTGGAAA TCTGGGGGGC CGAGTACCAG 
GAGTCGAACG CACTTCTGCT GAGGCCCTCT GACCGGGACT TCCTGAGTTG TGTCAGCGCC 
CGGGAACGCT GCCCAGTCTG CTTTGTGGGC ACCATCACTG GAGACAGGAG AATAGTGCTG 
GTGGATGATC GGGAGTGGCC TATGGGCACA GGTGGCCAGG TGGATGCGCC CCCCACTCCT 
CCCCCAACCC CTGTGGACCT GGACCTGGAC TGGGTGCTGG GCAAGATGCC CCAGAAGGAG 
TTCTTCCTCC AGAGGAGTCT CCCCTTGCTG CAGCCTCTGA CTCTGCCCCC AGGGCTGAGC 
GTGCGCCAGG CTCTGGCGCG GGTCCTCAGG CTGCCTGCCG TGGCCAGCAA GCGCTACCTC 
ACCAACAAGG TGGACCGTTC TGTGGGCGGC CTGGTGGCCC AGCAGCAGTG TGTGGGACCC 
CTGCAGACCC CTCTGGCAGA TGTGGCGGTT GTGGCACTGA GTCACCAGGA GCTTGTAGGG 
GCCGCCACAG CCCTGGGAGA GCAGCCAGTC AAGAGCCTGC TGGACCCGAA GGTTGCCGCC 
CGGCTGGCTG TGGCCGAAGC CCTCACCAAC CTGGTATTTG CTCTGGTCAC CGACCTCCAG 
GATGTGAAAT GCAGCGGGAA CTGGATGTGG GCAGCCAAGC TCCCAGGGGA GGGTGCAGCT 
CTGGCTGACG CCTGTGAGGC TATGGTGGCA GTGATGGCGG CCCTGGGTGT AGCGGTAGAC 
GGTGGCAAGG ACTCCCTCAG CATGGCTGCC CGGGTTGGCT CTGAAACCGT GCGGGCTCCT 
GGGTCACTGG TCATCTCAGC CTATGCTGTC TGTTCAGACA TTACAGCCAC TGTGACCCCA 
GACCTCAAGC GTCCTGGGGG GAGAGGCCAC CTGCTCTACG TGCCTCTGAG TCCTGGGCAG 
CACCGGCTGG GGGGCACAGC TCTGGCTCAG TGCTTCTCCC AGCTTGGCGA GCAGCCTCCC 
GACCTGGATG TTCCTGAGAA CTTGGTGCGA GCCTTCAGCA TCACCCAGGG GCTGCTGAAA 
GACCGCCTCC TCTGCTCAGG CCATGATGTC AGTGATGGAG GTCTCATCAC TTGCCTGCTA 
GAGATGGCCT TTGCTGGGAA TTGTGGGATA GAGGTGGACA TCCCCGCACC TGGGGTCGAT 
GCCCTGCCTG TGCTGTTCGC CGAGGAGCCA GGCCTGGTGC TGGAGGTGCA GGAGCCGGAC 
CTCGCCCAAG TGCTGATGCG TTACCGGAAT GCTGGCCTCC ACTGCCTAGA GCTGGGTCTC 
ACAGGCGACA CGGGGCCCCA CGCCATGGTC CGCGTGTCGG TGAACAGGAC GGTGGTTCTG 
GAGGAACCTG TTGGGCAGCT ACGAGCCCTC TGGGAGGAAA CCAGTTTCCA GTTGGACCGG 
CTACAGGCAA AACCACACTG TGTGGCCCAG GAAGAGCAAG GGCTGAGGGA GCGGGCAGGG 
CCCACCTTCT GCCTGCCGCC TACCTTCCCC AGAGCTTCCG TGCCTTGTGA GCCTGGTGGT 
CCTGCCCCCC GAGTCGCCAT CATTCGGGAA GAGGGCAGTA ATGGAGACCG GGAGATGGCA 
GATGCCTTCC ACTTGGCTGG ATTTGAGGTG TGGGATGTAA CCATGCAGGA CCTGTGCTCT 
GGAGCCATCG GGCTGGACAC ATTCCGCGGT GTGGCCTTCG TAGGCGGCTT CAGCTACGCG 
GACGTCCTGG GCTCTGCCAA AGGCTGGGCA GCTGCTGTGA CCTTTAACCC GCAGGCCGGG 
GCTGAGATGA AGCGCTTCCG CCAGCGGCCA GACACCTTCA GCCTGGGCGT GTGTAACGGC 
TGTCAGCTGC TGGCCCTGCT GGGCTGGGTG GGAGGCAGTT CTAGTGAGGA GGTGGTGGAG 
ATGTGCCAGG AGTCCTGGCC GGCCCGGCCC GGTCTCCTGC TACGCCACAA CCTGTCTGGG 
CGCTTTGAGT CGCGCTGGGC TAGTGTGCGC GTGGGGCCCG GGCCAGCCCT GATGCTGCGA 
GGGATGGAAG GCGCGGTGCT GCCCGTGTGG AGCGCTCACG GGGAAGGTTA CATGGCATTT 
TCTTCTCCGG AACTCCAAGC CCAGATGGAG GCCAGGGGCT TAGCTCCACT GCACTGGGCA 
GATGATGATG GGAACCCCAC GGAACAGTAC CCCCTGAATC CCAACGGCTC CCCAGGGGGT 
GTCGCTGGCA TCTGCTCCCC CGATGGCCGC CACTTGGCCC TCATGCCTCA CCCGGAGCGG 
TCTGTGAGGC CTTGGCAGTG GGCCTGGCGA CCTCCTCCAT TTGACACTCT AACCACCTCC 
CCCTGGCTCC AGCTCTTCAT CAATGCCCGG AATTGGACCC AGGAATGCAG CTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFAS ( XM_024980168.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024835936.1 Bos taurus phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa226375
Clone ID Related Accession (Same CDS sequence)	XM_024980167.1 , XM_024980168.1
Accession Version	XM_024980168.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	phosphoribosylformylglycinamidine synthase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTCCG TCCTTCACTT CTATGTCCGC CCCTCTGGCC ACGAGAGGGC AGCCTCTGAA 
TACATTCAAA GGAAGCTGCA AAGGGAACTG CCAGAGCTGC AGGGTGTCAA GACCGAGCAG 
TGCTACAATG TGAACTGGAC AGCGGAGTCC TTCCCGAGCA ACAAGGAGAT GAAGAAGCTG 
ACATGGCTAT TTGGCTGCCC CTTGTTACTG GACGATGTTG CTCAGGAATC CTGGCTTCGT 
CCTGGCCCCA CAGACCTGCT GCTGGAGGTC GGACCCAGGC TGAACTTTTC CACCCCGACA 
TCCAGCAACA TTGTGTCAGT GTGCCAGGCT GCAGGGCTGG GGGCTGTGGA CCGCGTGGAG 
CCAACCCGGC GCTACCTGCT CTCGTTTGCC CACCCACCTT CAGCTGACCT GGAGGCCATT 
GCCCTGGCTA CCCTGCATGA CCGGATGACA GAGCAGCGCT TCCCCCAGCC CATCCAGAGC 
TTCTCTTCAG GGTGCATCCC AGCACCCCTG AGCGGCCCAA TTGACGTGCT GGCTGAGGGC 
CGGTCTGCCC TGGAGAAAGC CAACCAGGAG CTAGGTCTGG CCCTAGACTC CTGGGACCTG 
GATTTCTACA CCAAGCGCTT CCAGGAGCTG CAGCGCAACC CCAGCACTGT GGAAGTCTTC 
GACTTGGCTC AGTCCAATAG CGAACACAGC CGACACTGGT TCTTCAAGGG TCGACTCCAT 
GTGGATGGAC AGGAGCTGCC ACACTCGCTG TTTGAGTCCA TCATGAGCAC CCAGGCGTCC 
TCCAACCCTA ACAACGTCCT CAAGTTCTGT GACAACAGCA GTGCCATCCA GGGGAAAGAA 
GTCCGATTCT TGCGGCCTAA GGACCCCACA CGGCCAAGCT CCTTCCAGCA ATGTCAGGGG 
CTGAGACATG TTGTCTTCAC AGCAGAGACA CACAACTTCC CTACAGGAGT AGCCCCCTTC 
AGTGGTGCAA CCACTGGCAC AGGGGGCCGG ATCCGAGATG TCCAGTGCAC AGGCCGAGGG 
GCCCACGTGG TGGCTGGCAC TGCTGGCTAT TGCTTTGGAA ACCTGCACAT CCCAGGTTAC 
AGTATGCCCT GGGAGGATCC AAGCTTCCAG TATCCAGGAA ACTTTGCCCG ACCCCTGGAG 
ATTGCCATTG AGGCCAGTAA TGGGGCTTCT GACTACGGCA ACAAGTTTGG GGAACCAGTG 
CTTGCTGGCT TTGCCCGCTC CTTGGGCCTT CAGCTCCCGG ACGGCCAGCG GCGTGAGTGG 
ATCAAGCCCA TCATGTTTAG TGGAGGCATT GGGTCCATGG AAGCTGATCA TGTGAGCAAG 
GAGCCCCCAG AGACAGGCAT GGATGTCGTG AAGATTGGCG GTCCTGTCTA CAGGATTGGA 
GTTGGGGGCG GAGCTGCTTC GTCTGTGCAG GTGCAAGGAG ACAACGCCAG TGACCTGGAT 
TTTGGGGCTG TGCAGCGGGG AGACCCAGAA ATGGAGCAGA AGATGAACCG TGTGATCCGG 
GCTTGTGTGG AGGCCCCTGG GGGAAACCCC ATCTGCAGCC TCCATGACCA GGGTGCTGGT 
GGCAATGGCA ATGTCCTGAA GGAGCTGAGT GACCCGGCTG GAGCCATCAT TCACACCAGC 
TGCTTCCAGC TGGGGGACCC AACCCTGAAT GCCCTGGAAA TCTGGGGGGC CGAGTACCAG 
GAGTCGAACG CACTTCTGCT GAGGCCCTCT GACCGGGACT TCCTGAGTTG TGTCAGCGCC 
CGGGAACGCT GCCCAGTCTG CTTTGTGGGC ACCATCACTG GAGACAGGAG AATAGTGCTG 
GTGGATGATC GGGAGTGGCC TATGGGCACA GGTGGCCAGG TGGATGCGCC CCCCACTCCT 
CCCCCAACCC CTGTGGACCT GGACCTGGAC TGGGTGCTGG GCAAGATGCC CCAGAAGGAG 
TTCTTCCTCC AGAGGAGTCT CCCCTTGCTG CAGCCTCTGA CTCTGCCCCC AGGGCTGAGC 
GTGCGCCAGG CTCTGGCGCG GGTCCTCAGG CTGCCTGCCG TGGCCAGCAA GCGCTACCTC 
ACCAACAAGG TGGACCGTTC TGTGGGCGGC CTGGTGGCCC AGCAGCAGTG TGTGGGACCC 
CTGCAGACCC CTCTGGCAGA TGTGGCGGTT GTGGCACTGA GTCACCAGGA GCTTGTAGGG 
GCCGCCACAG CCCTGGGAGA GCAGCCAGTC AAGAGCCTGC TGGACCCGAA GGTTGCCGCC 
CGGCTGGCTG TGGCCGAAGC CCTCACCAAC CTGGTATTTG CTCTGGTCAC CGACCTCCAG 
GATGTGAAAT GCAGCGGGAA CTGGATGTGG GCAGCCAAGC TCCCAGGGGA GGGTGCAGCT 
CTGGCTGACG CCTGTGAGGC TATGGTGGCA GTGATGGCGG CCCTGGGTGT AGCGGTAGAC 
GGTGGCAAGG ACTCCCTCAG CATGGCTGCC CGGGTTGGCT CTGAAACCGT GCGGGCTCCT 
GGGTCACTGG TCATCTCAGC CTATGCTGTC TGTTCAGACA TTACAGCCAC TGTGACCCCA 
GACCTCAAGC GTCCTGGGGG GAGAGGCCAC CTGCTCTACG TGCCTCTGAG TCCTGGGCAG 
CACCGGCTGG GGGGCACAGC TCTGGCTCAG TGCTTCTCCC AGCTTGGCGA GCAGCCTCCC 
GACCTGGATG TTCCTGAGAA CTTGGTGCGA GCCTTCAGCA TCACCCAGGG GCTGCTGAAA 
GACCGCCTCC TCTGCTCAGG CCATGATGTC AGTGATGGAG GTCTCATCAC TTGCCTGCTA 
GAGATGGCCT TTGCTGGGAA TTGTGGGATA GAGGTGGACA TCCCCGCACC TGGGGTCGAT 
GCCCTGCCTG TGCTGTTCGC CGAGGAGCCA GGCCTGGTGC TGGAGGTGCA GGAGCCGGAC 
CTCGCCCAAG TGCTGATGCG TTACCGGAAT GCTGGCCTCC ACTGCCTAGA GCTGGGTCTC 
ACAGGCGACA CGGGGCCCCA CGCCATGGTC CGCGTGTCGG TGAACAGGAC GGTGGTTCTG 
GAGGAACCTG TTGGGCAGCT ACGAGCCCTC TGGGAGGAAA CCAGTTTCCA GTTGGACCGG 
CTACAGGCAA AACCACACTG TGTGGCCCAG GAAGAGCAAG GGCTGAGGGA GCGGGCAGGG 
CCCACCTTCT GCCTGCCGCC TACCTTCCCC AGAGCTTCCG TGCCTTGTGA GCCTGGTGGT 
CCTGCCCCCC GAGTCGCCAT CATTCGGGAA GAGGGCAGTA ATGGAGACCG GGAGATGGCA 
GATGCCTTCC ACTTGGCTGG ATTTGAGGTG TGGGATGTAA CCATGCAGGA CCTGTGCTCT 
GGAGCCATCG GGCTGGACAC ATTCCGCGGT GTGGCCTTCG TAGGCGGCTT CAGCTACGCG 
GACGTCCTGG GCTCTGCCAA AGGCTGGGCA GCTGCTGTGA CCTTTAACCC GCAGGCCGGG 
GCTGAGATGA AGCGCTTCCG CCAGCGGCCA GACACCTTCA GCCTGGGCGT GTGTAACGGC 
TGTCAGCTGC TGGCCCTGCT GGGCTGGGTG GGAGGCAGTT CTAGTGAGGA GGTGGTGGAG 
ATGTGCCAGG AGTCCTGGCC GGCCCGGCCC GGTCTCCTGC TACGCCACAA CCTGTCTGGG 
CGCTTTGAGT CGCGCTGGGC TAGTGTGCGC GTGGGGCCCG GGCCAGCCCT GATGCTGCGA 
GGGATGGAAG GCGCGGTGCT GCCCGTGTGG AGCGCTCACG GGGAAGGTTA CATGGCATTT 
TCTTCTCCGG AACTCCAAGC CCAGATGGAG GCCAGGGGCT TAGCTCCACT GCACTGGGCA 
GATGATGATG GGAACCCCAC GGAACAGTAC CCCCTGAATC CCAACGGCTC CCCAGGGGGT 
GTCGCTGGCA TCTGCTCCCC CGATGGCCGC CACTTGGCCC TCATGCCTCA CCCGGAGCGG 
TCTGTGAGGC CTTGGCAGTG GGCCTGGCGA CCTCCTCCAT TTGACACTCT AACCACCTCC 
CCCTGGCTCC AGCTCTTCAT CAATGCCCGG AATTGGACCC AGGAATGCAG CTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024835936.1
CDS	206..4222
Translation	MSSVLHFYVRPSGHERAASEYIQRKLQRELPELQGVKTEQCYNVNWTAESFPSNKEMKKLTWLFGCPLLLDDVAQESWLRPGPTDLLLEVGPRLNFSTPTSSNIVSVCQAAGLGAVDRVEPTRRYLLSFAHPPSADLEAIALATLHDRMTEQRFPQPIQSFSSGCIPAPLSGPIDVLAEGRSALEKANQELGLALDSWDLDFYTKRFQELQRNPSTVEVFDLAQSNSEHSRHWFFKGRLHVDGQELPHSLFESIMSTQASSNPNNVLKFCDNSSAIQGKEVRFLRPKDPTRPSSFQQCQGLRHVVFTAETHNFPTGVAPFSGATTGTGGRIRDVQCTGRGAHVVAGTAGYCFGNLHIPGYSMPWEDPSFQYPGNFARPLEIAIEASNGASDYGNKFGEPVLAGFARSLGLQLPDGQRREWIKPIMFSGGIGSMEADHVSKEPPETGMDVVKIGGPVYRIGVGGGAASSVQVQGDNASDLDFGAVQRGDPEMEQKMNRVIRACVEAPGGNPICSLHDQGAGGNGNVLKELSDPAGAIIHTSCFQLGDPTLNALEIWGAEYQESNALLLRPSDRDFLSCVSARERCPVCFVGTITGDRRIVLVDDREWPMGTGGQVDAPPTPPPTPVDLDLDWVLGKMPQKEFFLQRSLPLLQPLTLPPGLSVRQALARVLRLPAVASKRYLTNKVDRSVGGLVAQQQCVGPLQTPLADVAVVALSHQELVGAATALGEQPVKSLLDPKVAARLAVAEALTNLVFALVTDLQDVKCSGNWMWAAKLPGEGAALADACEAMVAVMAALGVAVDGGKDSLSMAARVGSETVRAPGSLVISAYAVCSDITATVTPDLKRPGGRGHLLYVPLSPGQHRLGGTALAQCFSQLGEQPPDLDVPENLVRAFSITQGLLKDRLLCSGHDVSDGGLITCLLEMAFAGNCGIEVDIPAPGVDALPVLFAEEPGLVLEVQEPDLAQVLMRYRNAGLHCLELGLTGDTGPHAMVRVSVNRTVVLEEPVGQLRALWEETSFQLDRLQAKPHCVAQEEQGLRERAGPTFCLPPTFPRASVPCEPGGPAPRVAIIREEGSNGDREMADAFHLAGFEVWDVTMQDLCSGAIGLDTFRGVAFVGGFSYADVLGSAKGWAAAVTFNPQAGAEMKRFRQRPDTFSLGVCNGCQLLALLGWVGGSSSEEVVEMCQESWPARPGLLLRHNLSGRFESRWASVRVGPGPALMLRGMEGAVLPVWSAHGEGYMAFSSPELQAQMEARGLAPLHWADDDGNPTEQYPLNPNGSPGGVAGICSPDGRHLALMPHPERSVRPWQWAWRPPPFDTLTTSPWLQLFINARNWTQECSC

Target ORF information:

RefSeq Version	XM_024980168.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024980168.1

ORF Insert Sequence:

ATGTCCTCCG TCCTTCACTT CTATGTCCGC CCCTCTGGCC ACGAGAGGGC AGCCTCTGAA 
TACATTCAAA GGAAGCTGCA AAGGGAACTG CCAGAGCTGC AGGGTGTCAA GACCGAGCAG 
TGCTACAATG TGAACTGGAC AGCGGAGTCC TTCCCGAGCA ACAAGGAGAT GAAGAAGCTG 
ACATGGCTAT TTGGCTGCCC CTTGTTACTG GACGATGTTG CTCAGGAATC CTGGCTTCGT 
CCTGGCCCCA CAGACCTGCT GCTGGAGGTC GGACCCAGGC TGAACTTTTC CACCCCGACA 
TCCAGCAACA TTGTGTCAGT GTGCCAGGCT GCAGGGCTGG GGGCTGTGGA CCGCGTGGAG 
CCAACCCGGC GCTACCTGCT CTCGTTTGCC CACCCACCTT CAGCTGACCT GGAGGCCATT 
GCCCTGGCTA CCCTGCATGA CCGGATGACA GAGCAGCGCT TCCCCCAGCC CATCCAGAGC 
TTCTCTTCAG GGTGCATCCC AGCACCCCTG AGCGGCCCAA TTGACGTGCT GGCTGAGGGC 
CGGTCTGCCC TGGAGAAAGC CAACCAGGAG CTAGGTCTGG CCCTAGACTC CTGGGACCTG 
GATTTCTACA CCAAGCGCTT CCAGGAGCTG CAGCGCAACC CCAGCACTGT GGAAGTCTTC 
GACTTGGCTC AGTCCAATAG CGAACACAGC CGACACTGGT TCTTCAAGGG TCGACTCCAT 
GTGGATGGAC AGGAGCTGCC ACACTCGCTG TTTGAGTCCA TCATGAGCAC CCAGGCGTCC 
TCCAACCCTA ACAACGTCCT CAAGTTCTGT GACAACAGCA GTGCCATCCA GGGGAAAGAA 
GTCCGATTCT TGCGGCCTAA GGACCCCACA CGGCCAAGCT CCTTCCAGCA ATGTCAGGGG 
CTGAGACATG TTGTCTTCAC AGCAGAGACA CACAACTTCC CTACAGGAGT AGCCCCCTTC 
AGTGGTGCAA CCACTGGCAC AGGGGGCCGG ATCCGAGATG TCCAGTGCAC AGGCCGAGGG 
GCCCACGTGG TGGCTGGCAC TGCTGGCTAT TGCTTTGGAA ACCTGCACAT CCCAGGTTAC 
AGTATGCCCT GGGAGGATCC AAGCTTCCAG TATCCAGGAA ACTTTGCCCG ACCCCTGGAG 
ATTGCCATTG AGGCCAGTAA TGGGGCTTCT GACTACGGCA ACAAGTTTGG GGAACCAGTG 
CTTGCTGGCT TTGCCCGCTC CTTGGGCCTT CAGCTCCCGG ACGGCCAGCG GCGTGAGTGG 
ATCAAGCCCA TCATGTTTAG TGGAGGCATT GGGTCCATGG AAGCTGATCA TGTGAGCAAG 
GAGCCCCCAG AGACAGGCAT GGATGTCGTG AAGATTGGCG GTCCTGTCTA CAGGATTGGA 
GTTGGGGGCG GAGCTGCTTC GTCTGTGCAG GTGCAAGGAG ACAACGCCAG TGACCTGGAT 
TTTGGGGCTG TGCAGCGGGG AGACCCAGAA ATGGAGCAGA AGATGAACCG TGTGATCCGG 
GCTTGTGTGG AGGCCCCTGG GGGAAACCCC ATCTGCAGCC TCCATGACCA GGGTGCTGGT 
GGCAATGGCA ATGTCCTGAA GGAGCTGAGT GACCCGGCTG GAGCCATCAT TCACACCAGC 
TGCTTCCAGC TGGGGGACCC AACCCTGAAT GCCCTGGAAA TCTGGGGGGC CGAGTACCAG 
GAGTCGAACG CACTTCTGCT GAGGCCCTCT GACCGGGACT TCCTGAGTTG TGTCAGCGCC 
CGGGAACGCT GCCCAGTCTG CTTTGTGGGC ACCATCACTG GAGACAGGAG AATAGTGCTG 
GTGGATGATC GGGAGTGGCC TATGGGCACA GGTGGCCAGG TGGATGCGCC CCCCACTCCT 
CCCCCAACCC CTGTGGACCT GGACCTGGAC TGGGTGCTGG GCAAGATGCC CCAGAAGGAG 
TTCTTCCTCC AGAGGAGTCT CCCCTTGCTG CAGCCTCTGA CTCTGCCCCC AGGGCTGAGC 
GTGCGCCAGG CTCTGGCGCG GGTCCTCAGG CTGCCTGCCG TGGCCAGCAA GCGCTACCTC 
ACCAACAAGG TGGACCGTTC TGTGGGCGGC CTGGTGGCCC AGCAGCAGTG TGTGGGACCC 
CTGCAGACCC CTCTGGCAGA TGTGGCGGTT GTGGCACTGA GTCACCAGGA GCTTGTAGGG 
GCCGCCACAG CCCTGGGAGA GCAGCCAGTC AAGAGCCTGC TGGACCCGAA GGTTGCCGCC 
CGGCTGGCTG TGGCCGAAGC CCTCACCAAC CTGGTATTTG CTCTGGTCAC CGACCTCCAG 
GATGTGAAAT GCAGCGGGAA CTGGATGTGG GCAGCCAAGC TCCCAGGGGA GGGTGCAGCT 
CTGGCTGACG CCTGTGAGGC TATGGTGGCA GTGATGGCGG CCCTGGGTGT AGCGGTAGAC 
GGTGGCAAGG ACTCCCTCAG CATGGCTGCC CGGGTTGGCT CTGAAACCGT GCGGGCTCCT 
GGGTCACTGG TCATCTCAGC CTATGCTGTC TGTTCAGACA TTACAGCCAC TGTGACCCCA 
GACCTCAAGC GTCCTGGGGG GAGAGGCCAC CTGCTCTACG TGCCTCTGAG TCCTGGGCAG 
CACCGGCTGG GGGGCACAGC TCTGGCTCAG TGCTTCTCCC AGCTTGGCGA GCAGCCTCCC 
GACCTGGATG TTCCTGAGAA CTTGGTGCGA GCCTTCAGCA TCACCCAGGG GCTGCTGAAA 
GACCGCCTCC TCTGCTCAGG CCATGATGTC AGTGATGGAG GTCTCATCAC TTGCCTGCTA 
GAGATGGCCT TTGCTGGGAA TTGTGGGATA GAGGTGGACA TCCCCGCACC TGGGGTCGAT 
GCCCTGCCTG TGCTGTTCGC CGAGGAGCCA GGCCTGGTGC TGGAGGTGCA GGAGCCGGAC 
CTCGCCCAAG TGCTGATGCG TTACCGGAAT GCTGGCCTCC ACTGCCTAGA GCTGGGTCTC 
ACAGGCGACA CGGGGCCCCA CGCCATGGTC CGCGTGTCGG TGAACAGGAC GGTGGTTCTG 
GAGGAACCTG TTGGGCAGCT ACGAGCCCTC TGGGAGGAAA CCAGTTTCCA GTTGGACCGG 
CTACAGGCAA AACCACACTG TGTGGCCCAG GAAGAGCAAG GGCTGAGGGA GCGGGCAGGG 
CCCACCTTCT GCCTGCCGCC TACCTTCCCC AGAGCTTCCG TGCCTTGTGA GCCTGGTGGT 
CCTGCCCCCC GAGTCGCCAT CATTCGGGAA GAGGGCAGTA ATGGAGACCG GGAGATGGCA 
GATGCCTTCC ACTTGGCTGG ATTTGAGGTG TGGGATGTAA CCATGCAGGA CCTGTGCTCT 
GGAGCCATCG GGCTGGACAC ATTCCGCGGT GTGGCCTTCG TAGGCGGCTT CAGCTACGCG 
GACGTCCTGG GCTCTGCCAA AGGCTGGGCA GCTGCTGTGA CCTTTAACCC GCAGGCCGGG 
GCTGAGATGA AGCGCTTCCG CCAGCGGCCA GACACCTTCA GCCTGGGCGT GTGTAACGGC 
TGTCAGCTGC TGGCCCTGCT GGGCTGGGTG GGAGGCAGTT CTAGTGAGGA GGTGGTGGAG 
ATGTGCCAGG AGTCCTGGCC GGCCCGGCCC GGTCTCCTGC TACGCCACAA CCTGTCTGGG 
CGCTTTGAGT CGCGCTGGGC TAGTGTGCGC GTGGGGCCCG GGCCAGCCCT GATGCTGCGA 
GGGATGGAAG GCGCGGTGCT GCCCGTGTGG AGCGCTCACG GGGAAGGTTA CATGGCATTT 
TCTTCTCCGG AACTCCAAGC CCAGATGGAG GCCAGGGGCT TAGCTCCACT GCACTGGGCA 
GATGATGATG GGAACCCCAC GGAACAGTAC CCCCTGAATC CCAACGGCTC CCCAGGGGGT 
GTCGCTGGCA TCTGCTCCCC CGATGGCCGC CACTTGGCCC TCATGCCTCA CCCGGAGCGG 
TCTGTGAGGC CTTGGCAGTG GGCCTGGCGA CCTCCTCCAT TTGACACTCT AACCACCTCC 
CCCTGGCTCC AGCTCTTCAT CAATGCCCGG AATTGGACCC AGGAATGCAG CTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFAS ( XM_015458726.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015314212.2 Bos taurus phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X2, mRNA.

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CloneID	OBa226376
Clone ID Related Accession (Same CDS sequence)	XM_015458726.2
Accession Version	XM_015458726.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	phosphoribosylformylglycinamidine synthase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015458726.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTCCG TCCTTCACTT CTATGTCCGC CCCTCTGGCC ACGAGAGGGC AGCCTCTGAA 
TACATTCAAA GGAAGCTGCA AAGGGAACTG CCAGAGCTGC AGGGTGTCAA GACCGAGCAG 
TGCTACAATG TGAACTGGAC AGCGGAGTCC TTCCCGAGCA ACAAGGAGAT GAAGAAGCTG 
ACATGGCTAT TTGGCTGCCC CTTGTTACTG GACGATGTTG CTCAGGAATC CTGGCTTCGT 
CCTGGCCCCA CAGACCTGCT GCTGGAGGTC GGACCCAGGC TGAACTTTTC CACCCCGACA 
TCCAGCAACA TTGTGTCAGT GTGCCAGGCT GCAGGGCTGG GGGCTGTGGA CCGCGTGGAG 
CCAACCCGGC GCTACCTGCT CTCGTTTGCC CACCCACCTT CAGCTGACCT GGAGGCCATT 
GCCCTGGCTA CCCTGCATGA CCGGATGACA GAGCAGCGCT TCCCCCAGCC CATCCAGAGC 
TTCTCTTCAG GGTGCATCCC AGCACCCCTG AGCGGCCCAA TTGACGTGCT GGCTGAGGGC 
CGGTCTGCCC TGGAGAAAGC CAACCAGGAG CTAGGTCTGG CCCTAGACTC CTGGGACCTG 
GATTTCTACA CCAAGCGCTT CCAGGAGCTG CAGCGCAACC CCAGCACTGT GGAAGTCTTC 
GACTTGGCTC AGTCCAATAG CGAACACAGC CGACACTGGT TCTTCAAGGG TCGACTCCAT 
GTGGATGGAC AGGAGCTGCC ACACTCGCTG TTTGAGTCCA TCATGAGCAC CCAGGCGTCC 
TCCAACCCTA ACAACGTCCT CAAGTTCTGT GACAACAGCA GTGCCATCCA GGGGAAAGAA 
GTCCGATTCT TGCGGCCTAA GGACCCCACA CGGCCAAGCT CCTTCCAGCA ATGTCAGGGG 
CTGAGACATG TTGTCTTCAC AGCAGAGACA CACAACTTCC CTACAGGAGT AGCCCCCTTC 
AGTGGTGCAA CCACTGGCAC AGGGGGCCGG ATCCGAGATG TCCAGTGCAC AGGCCGAGGG 
GCCCACGTGG TGGCTGGCAC TGCTGGCTAT TGCTTTGGAA ACCTGCACAT CCCAGGTTAC 
AGTATGCCCT GGGAGGATCC AAGCTTCCAG TATCCAGGAA ACTTTGCCCG ACCCCTGGAG 
ATTGCCATTG AGGCCAGTAA TGGGGCTTCT GACTACGGCA ACAAGTTTGG GGAACCAGTG 
CTTGCTGGCT TTGCCCGCTC CTTGGGCCTT CAGCTCCCGG ACGGCCAGCG GCGTGAGTGG 
ATCAAGCCCA TCATGTTTAG TGGAGGCATT GGGTCCATGG AAGCTGATCA TGTGAGCAAG 
GAGCCCCCAG AGACAGGCAT GGATGTCGTG AAGATTGGCG GTCCTGTCTA CAGGATTGGA 
GTTGGGGGCG GAGCTGCTTC GTCTGTGCAG GTGCAAGGAG ACAACGCCAG TGACCTGGAT 
TTTGGGGCTG TGCAGCGGGG AGACCCAGAA ATGGAGCAGA AGATGAACCG TGTGATCCGG 
GCTTGTGTGG AGGCCCCTGG GGGAAACCCC ATCTGCAGCC TCCATGACCA GGGTGCTGGT 
GGCAATGGCA ATGTCCTGAA GGAGCTGAGT GACCCGGCTG GAGCCATCAT TCACACCAGC 
TGCTTCCAGC TGGGGGACCC AACCCTGAAT GCCCTGGAAA TCTGGGGGGC CGAGTACCAG 
GAGTCGAACG CACTTCTGCT GAGGCCCTCT GACCGGGACT TCCTGAGTTG TGTCAGCGCC 
CGGGAACGCT GCCCAGTCTG CTTTGTGGGC ACCATCACTG GAGACAGGAG AATAGTGCTG 
GTGGATGATC GGGAGTGGCC TATGGGCACA GGTGGCCAGG TGGATGCGCC CCCCACTCCT 
CCCCCAACCC CTGTGGACCT GGACCTGGAC TGGGTGCTGG GCAAGATGCC CCAGAAGGAG 
TTCTTCCTCC AGAGGAGTCT CCCCTTGCTG CAGCCTCTGA CTCTGCCCCC AGGGCTGAGC 
GTGCGCCAGG CTCTGGCGCG GGTCCTCAGG CTGCCTGCCG TGGCCAGCAA GCGCTACCTC 
ACCAACAAGG TGGACCGTTC TGTGGGCGGC CTGGTGGCCC AGCAGCAGTG TGTGGGACCC 
CTGCAGACCC CTCTGGCAGA TGTGGCGGTT GTGGCACTGA GTCACCAGGA GCTTGTAGGG 
GCCGCCACAG CCCTGGGAGA GCAGCCAGTC AAGAGCCTGC TGGACCCGAA GGTTGCCGCC 
CGGCTGGCTG TGGCCGAAGC CCTCACCAAC CTGGTATTTG CTCTGGTCAC CGACCTCCAG 
GATGTGAAAT GCAGCGGGAA CTGGATGTGG GCAGCCAAGC TCCCAGGGGA GGGTGCAGCT 
CTGGCTGACG CCTGTGAGGC TATGGTGGCA GTGATGGCGG CCCTGGGTGT AGCGGTAGAC 
GGTGGCAAGG ACTCCCTCAG CATGGCTGCC CGGGTTGGCT CTGAAACCGT GCGGGCTCCT 
GGGTCACTGG TCATCTCAGC CTATGCTGTC TGTTCAGACA TTACAGCCAC TGTGACCCCA 
GACCTCAAGC GTCCTGGGGG GAGAGGCCAC CTGCTCTACG TGCCTCTGAG TCCTGGGCAG 
CACCGGCTGG GGGGCACAGC TCTGGCTCAG TGCTTCTCCC AGCTTGGCGA GCAGCCTCCC 
GACCTGGATG TTCCTGAGAA CTTGGTGCGA GCCTTCAGCA TCACCCAGGG GCTGCTGAAA 
GACCGCCTCC TCTGCTCAGG CCATGATGTC AGTGATGGAG GTCTCATCAC TTGCCTGCTA 
GAGATGGCCT TTGCTGGGAA TTGTGGGATA GAGGTGGACA TCCCCGCACC TGGGGTCGAT 
GCCCTGCCTG TGCTGTTCGC CGAGGAGCCA GGCCTGGTGC TGGAGGTGCA GGAGCCGGAC 
CTCGCCCAAG TGCTGATGCG TTACCGGAAT GCTGGCCTCC ACTGCCTAGA GCTGGGTCTC 
ACAGGCGACA CGGGGCCCCA CGCCATGGTC CGCGTGTCGG TGAACAGGAC GGTGGTTCTG 
GAGGAACCTG TTGGGCAGCT ACGAGCCCTC TGGGAGGAAA CCAGTTTCCA GTTGGACCGG 
CTACAGGCAA AACCACACTG TGTGGCCCAG GAAGAGCAAG GGCTGAGGGA GCGGGCAGGG 
CCCACCTTCT GCCTGCCGCC TACCTTCCCC AGAGCTTCCG TGCCTTGTGG TCCTGCCCCC 
CGAGTCGCCA TCATTCGGGA AGAGGGCAGT AATGGAGACC GGGAGATGGC AGATGCCTTC 
CACTTGGCTG GATTTGAGGT GTGGGATGTA ACCATGCAGG ACCTGTGCTC TGGAGCCATC 
GGGCTGGACA CATTCCGCGG TGTGGCCTTC GTAGGCGGCT TCAGCTACGC GGACGTCCTG 
GGCTCTGCCA AAGGCTGGGC AGCTGCTGTG ACCTTTAACC CGCAGGCCGG GGCTGAGATG 
AAGCGCTTCC GCCAGCGGCC AGACACCTTC AGCCTGGGCG TGTGTAACGG CTGTCAGCTG 
CTGGCCCTGC TGGGCTGGGT GGGAGGCAGT TCTAGTGAGG AGGTGGTGGA GATGTGCCAG 
GAGTCCTGGC CGGCCCGGCC CGGTCTCCTG CTACGCCACA ACCTGTCTGG GCGCTTTGAG 
TCGCGCTGGG CTAGTGTGCG CGTGGGGCCC GGGCCAGCCC TGATGCTGCG AGGGATGGAA 
GGCGCGGTGC TGCCCGTGTG GAGCGCTCAC GGGGAAGGTT ACATGGCATT TTCTTCTCCG 
GAACTCCAAG CCCAGATGGA GGCCAGGGGC TTAGCTCCAC TGCACTGGGC AGATGATGAT 
GGGAACCCCA CGGAACAGTA CCCCCTGAAT CCCAACGGCT CCCCAGGGGG TGTCGCTGGC 
ATCTGCTCCC CCGATGGCCG CCACTTGGCC CTCATGCCTC ACCCGGAGCG GTCTGTGAGG 
CCTTGGCAGT GGGCCTGGCG ACCTCCTCCA TTTGACACTC TAACCACCTC CCCCTGGCTC 
CAGCTCTTCA TCAATGCCCG GAATTGGACC CAGGAATGCA GCTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015314212.2
CDS	81..4088
Translation	MSSVLHFYVRPSGHERAASEYIQRKLQRELPELQGVKTEQCYNVNWTAESFPSNKEMKKLTWLFGCPLLLDDVAQESWLRPGPTDLLLEVGPRLNFSTPTSSNIVSVCQAAGLGAVDRVEPTRRYLLSFAHPPSADLEAIALATLHDRMTEQRFPQPIQSFSSGCIPAPLSGPIDVLAEGRSALEKANQELGLALDSWDLDFYTKRFQELQRNPSTVEVFDLAQSNSEHSRHWFFKGRLHVDGQELPHSLFESIMSTQASSNPNNVLKFCDNSSAIQGKEVRFLRPKDPTRPSSFQQCQGLRHVVFTAETHNFPTGVAPFSGATTGTGGRIRDVQCTGRGAHVVAGTAGYCFGNLHIPGYSMPWEDPSFQYPGNFARPLEIAIEASNGASDYGNKFGEPVLAGFARSLGLQLPDGQRREWIKPIMFSGGIGSMEADHVSKEPPETGMDVVKIGGPVYRIGVGGGAASSVQVQGDNASDLDFGAVQRGDPEMEQKMNRVIRACVEAPGGNPICSLHDQGAGGNGNVLKELSDPAGAIIHTSCFQLGDPTLNALEIWGAEYQESNALLLRPSDRDFLSCVSARERCPVCFVGTITGDRRIVLVDDREWPMGTGGQVDAPPTPPPTPVDLDLDWVLGKMPQKEFFLQRSLPLLQPLTLPPGLSVRQALARVLRLPAVASKRYLTNKVDRSVGGLVAQQQCVGPLQTPLADVAVVALSHQELVGAATALGEQPVKSLLDPKVAARLAVAEALTNLVFALVTDLQDVKCSGNWMWAAKLPGEGAALADACEAMVAVMAALGVAVDGGKDSLSMAARVGSETVRAPGSLVISAYAVCSDITATVTPDLKRPGGRGHLLYVPLSPGQHRLGGTALAQCFSQLGEQPPDLDVPENLVRAFSITQGLLKDRLLCSGHDVSDGGLITCLLEMAFAGNCGIEVDIPAPGVDALPVLFAEEPGLVLEVQEPDLAQVLMRYRNAGLHCLELGLTGDTGPHAMVRVSVNRTVVLEEPVGQLRALWEETSFQLDRLQAKPHCVAQEEQGLRERAGPTFCLPPTFPRASVPCGPAPRVAIIREEGSNGDREMADAFHLAGFEVWDVTMQDLCSGAIGLDTFRGVAFVGGFSYADVLGSAKGWAAAVTFNPQAGAEMKRFRQRPDTFSLGVCNGCQLLALLGWVGGSSSEEVVEMCQESWPARPGLLLRHNLSGRFESRWASVRVGPGPALMLRGMEGAVLPVWSAHGEGYMAFSSPELQAQMEARGLAPLHWADDDGNPTEQYPLNPNGSPGGVAGICSPDGRHLALMPHPERSVRPWQWAWRPPPFDTLTTSPWLQLFINARNWTQECSC

Target ORF information:

RefSeq Version	XM_015458726.2
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015458726.2

ORF Insert Sequence:

ATGTCCTCCG TCCTTCACTT CTATGTCCGC CCCTCTGGCC ACGAGAGGGC AGCCTCTGAA 
TACATTCAAA GGAAGCTGCA AAGGGAACTG CCAGAGCTGC AGGGTGTCAA GACCGAGCAG 
TGCTACAATG TGAACTGGAC AGCGGAGTCC TTCCCGAGCA ACAAGGAGAT GAAGAAGCTG 
ACATGGCTAT TTGGCTGCCC CTTGTTACTG GACGATGTTG CTCAGGAATC CTGGCTTCGT 
CCTGGCCCCA CAGACCTGCT GCTGGAGGTC GGACCCAGGC TGAACTTTTC CACCCCGACA 
TCCAGCAACA TTGTGTCAGT GTGCCAGGCT GCAGGGCTGG GGGCTGTGGA CCGCGTGGAG 
CCAACCCGGC GCTACCTGCT CTCGTTTGCC CACCCACCTT CAGCTGACCT GGAGGCCATT 
GCCCTGGCTA CCCTGCATGA CCGGATGACA GAGCAGCGCT TCCCCCAGCC CATCCAGAGC 
TTCTCTTCAG GGTGCATCCC AGCACCCCTG AGCGGCCCAA TTGACGTGCT GGCTGAGGGC 
CGGTCTGCCC TGGAGAAAGC CAACCAGGAG CTAGGTCTGG CCCTAGACTC CTGGGACCTG 
GATTTCTACA CCAAGCGCTT CCAGGAGCTG CAGCGCAACC CCAGCACTGT GGAAGTCTTC 
GACTTGGCTC AGTCCAATAG CGAACACAGC CGACACTGGT TCTTCAAGGG TCGACTCCAT 
GTGGATGGAC AGGAGCTGCC ACACTCGCTG TTTGAGTCCA TCATGAGCAC CCAGGCGTCC 
TCCAACCCTA ACAACGTCCT CAAGTTCTGT GACAACAGCA GTGCCATCCA GGGGAAAGAA 
GTCCGATTCT TGCGGCCTAA GGACCCCACA CGGCCAAGCT CCTTCCAGCA ATGTCAGGGG 
CTGAGACATG TTGTCTTCAC AGCAGAGACA CACAACTTCC CTACAGGAGT AGCCCCCTTC 
AGTGGTGCAA CCACTGGCAC AGGGGGCCGG ATCCGAGATG TCCAGTGCAC AGGCCGAGGG 
GCCCACGTGG TGGCTGGCAC TGCTGGCTAT TGCTTTGGAA ACCTGCACAT CCCAGGTTAC 
AGTATGCCCT GGGAGGATCC AAGCTTCCAG TATCCAGGAA ACTTTGCCCG ACCCCTGGAG 
ATTGCCATTG AGGCCAGTAA TGGGGCTTCT GACTACGGCA ACAAGTTTGG GGAACCAGTG 
CTTGCTGGCT TTGCCCGCTC CTTGGGCCTT CAGCTCCCGG ACGGCCAGCG GCGTGAGTGG 
ATCAAGCCCA TCATGTTTAG TGGAGGCATT GGGTCCATGG AAGCTGATCA TGTGAGCAAG 
GAGCCCCCAG AGACAGGCAT GGATGTCGTG AAGATTGGCG GTCCTGTCTA CAGGATTGGA 
GTTGGGGGCG GAGCTGCTTC GTCTGTGCAG GTGCAAGGAG ACAACGCCAG TGACCTGGAT 
TTTGGGGCTG TGCAGCGGGG AGACCCAGAA ATGGAGCAGA AGATGAACCG TGTGATCCGG 
GCTTGTGTGG AGGCCCCTGG GGGAAACCCC ATCTGCAGCC TCCATGACCA GGGTGCTGGT 
GGCAATGGCA ATGTCCTGAA GGAGCTGAGT GACCCGGCTG GAGCCATCAT TCACACCAGC 
TGCTTCCAGC TGGGGGACCC AACCCTGAAT GCCCTGGAAA TCTGGGGGGC CGAGTACCAG 
GAGTCGAACG CACTTCTGCT GAGGCCCTCT GACCGGGACT TCCTGAGTTG TGTCAGCGCC 
CGGGAACGCT GCCCAGTCTG CTTTGTGGGC ACCATCACTG GAGACAGGAG AATAGTGCTG 
GTGGATGATC GGGAGTGGCC TATGGGCACA GGTGGCCAGG TGGATGCGCC CCCCACTCCT 
CCCCCAACCC CTGTGGACCT GGACCTGGAC TGGGTGCTGG GCAAGATGCC CCAGAAGGAG 
TTCTTCCTCC AGAGGAGTCT CCCCTTGCTG CAGCCTCTGA CTCTGCCCCC AGGGCTGAGC 
GTGCGCCAGG CTCTGGCGCG GGTCCTCAGG CTGCCTGCCG TGGCCAGCAA GCGCTACCTC 
ACCAACAAGG TGGACCGTTC TGTGGGCGGC CTGGTGGCCC AGCAGCAGTG TGTGGGACCC 
CTGCAGACCC CTCTGGCAGA TGTGGCGGTT GTGGCACTGA GTCACCAGGA GCTTGTAGGG 
GCCGCCACAG CCCTGGGAGA GCAGCCAGTC AAGAGCCTGC TGGACCCGAA GGTTGCCGCC 
CGGCTGGCTG TGGCCGAAGC CCTCACCAAC CTGGTATTTG CTCTGGTCAC CGACCTCCAG 
GATGTGAAAT GCAGCGGGAA CTGGATGTGG GCAGCCAAGC TCCCAGGGGA GGGTGCAGCT 
CTGGCTGACG CCTGTGAGGC TATGGTGGCA GTGATGGCGG CCCTGGGTGT AGCGGTAGAC 
GGTGGCAAGG ACTCCCTCAG CATGGCTGCC CGGGTTGGCT CTGAAACCGT GCGGGCTCCT 
GGGTCACTGG TCATCTCAGC CTATGCTGTC TGTTCAGACA TTACAGCCAC TGTGACCCCA 
GACCTCAAGC GTCCTGGGGG GAGAGGCCAC CTGCTCTACG TGCCTCTGAG TCCTGGGCAG 
CACCGGCTGG GGGGCACAGC TCTGGCTCAG TGCTTCTCCC AGCTTGGCGA GCAGCCTCCC 
GACCTGGATG TTCCTGAGAA CTTGGTGCGA GCCTTCAGCA TCACCCAGGG GCTGCTGAAA 
GACCGCCTCC TCTGCTCAGG CCATGATGTC AGTGATGGAG GTCTCATCAC TTGCCTGCTA 
GAGATGGCCT TTGCTGGGAA TTGTGGGATA GAGGTGGACA TCCCCGCACC TGGGGTCGAT 
GCCCTGCCTG TGCTGTTCGC CGAGGAGCCA GGCCTGGTGC TGGAGGTGCA GGAGCCGGAC 
CTCGCCCAAG TGCTGATGCG TTACCGGAAT GCTGGCCTCC ACTGCCTAGA GCTGGGTCTC 
ACAGGCGACA CGGGGCCCCA CGCCATGGTC CGCGTGTCGG TGAACAGGAC GGTGGTTCTG 
GAGGAACCTG TTGGGCAGCT ACGAGCCCTC TGGGAGGAAA CCAGTTTCCA GTTGGACCGG 
CTACAGGCAA AACCACACTG TGTGGCCCAG GAAGAGCAAG GGCTGAGGGA GCGGGCAGGG 
CCCACCTTCT GCCTGCCGCC TACCTTCCCC AGAGCTTCCG TGCCTTGTGG TCCTGCCCCC 
CGAGTCGCCA TCATTCGGGA AGAGGGCAGT AATGGAGACC GGGAGATGGC AGATGCCTTC 
CACTTGGCTG GATTTGAGGT GTGGGATGTA ACCATGCAGG ACCTGTGCTC TGGAGCCATC 
GGGCTGGACA CATTCCGCGG TGTGGCCTTC GTAGGCGGCT TCAGCTACGC GGACGTCCTG 
GGCTCTGCCA AAGGCTGGGC AGCTGCTGTG ACCTTTAACC CGCAGGCCGG GGCTGAGATG 
AAGCGCTTCC GCCAGCGGCC AGACACCTTC AGCCTGGGCG TGTGTAACGG CTGTCAGCTG 
CTGGCCCTGC TGGGCTGGGT GGGAGGCAGT TCTAGTGAGG AGGTGGTGGA GATGTGCCAG 
GAGTCCTGGC CGGCCCGGCC CGGTCTCCTG CTACGCCACA ACCTGTCTGG GCGCTTTGAG 
TCGCGCTGGG CTAGTGTGCG CGTGGGGCCC GGGCCAGCCC TGATGCTGCG AGGGATGGAA 
GGCGCGGTGC TGCCCGTGTG GAGCGCTCAC GGGGAAGGTT ACATGGCATT TTCTTCTCCG 
GAACTCCAAG CCCAGATGGA GGCCAGGGGC TTAGCTCCAC TGCACTGGGC AGATGATGAT 
GGGAACCCCA CGGAACAGTA CCCCCTGAAT CCCAACGGCT CCCCAGGGGG TGTCGCTGGC 
ATCTGCTCCC CCGATGGCCG CCACTTGGCC CTCATGCCTC ACCCGGAGCG GTCTGTGAGG 
CCTTGGCAGT GGGCCTGGCG ACCTCCTCCA TTTGACACTC TAACCACCTC CCCCTGGCTC 
CAGCTCTTCA TCAATGCCCG GAATTGGACC CAGGAATGCA GCTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFAS ( XM_005220359.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005220416.4 Bos taurus phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X3, mRNA.

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CloneID	OBa226377
Clone ID Related Accession (Same CDS sequence)	XM_005220359.4
Accession Version	XM_005220359.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4104bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	phosphoribosylformylglycinamidine synthase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005220359.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGATGA CGTTGATCTG CGCGCCAGAA TTCGAGGAGC TGACGGACTG GGAGAAGTGC 
TACCACGCGC AACGGACACC TGCAGAGATG TCCTCCGTCC TTCACTTCTA TGTCCGCCCC 
TCTGGCCACG AGAGGGCAGC CTCTGAATAC ATTCAAAGGA AGCTGCAAAG GGAACTGCCA 
GAGCTGCAGG GTGTCAAGAC CGAGCAGTGC TACAATGTGA ACTGGACAGC GGAGTCCTTC 
CCGAGCAACA AGGAGATGAA GAAGCTGACA TGGCTATTTG GCTGCCCCTT GTTACTGGAC 
GATGTTGCTC AGGAATCCTG GCTTCGTCCT GGCCCCACAG ACCTGCTGCT GGAGGTCGGA 
CCCAGGCTGA ACTTTTCCAC CCCGACATCC AGCAACATTG TGTCAGTGTG CCAGGCTGCA 
GGGCTGGGGG CTGTGGACCG CGTGGAGCCA ACCCGGCGCT ACCTGCTCTC GTTTGCCCAC 
CCACCTTCAG CTGACCTGGA GGCCATTGCC CTGGCTACCC TGCATGACCG GATGACAGAG 
CAGCGCTTCC CCCAGCCCAT CCAGAGCTTC TCTTCAGGGT GCATCCCAGC ACCCCTGAGC 
GGCCCAATTG ACGTGCTGGC TGAGGGCCGG TCTGCCCTGG AGAAAGCCAA CCAGGAGCTA 
GGTCTGGCCC TAGACTCCTG GGACCTGGAT TTCTACACCA AGCGCTTCCA GGAGCTGCAG 
CGCAACCCCA GCACTGTGGA AGTCTTCGAC TTGGCTCAGT CCAATAGCGA ACACAGCCGA 
CACTGGTTCT TCAAGGGTCG ACTCCATGTG GATGGACAGG AGCTGCCACA CTCGCTGTTT 
GAGTCCATCA TGAGCACCCA GGCGTCCTCC AACCCTAACA ACGTCCTCAA GTTCTGTGAC 
AACAGCAGTG CCATCCAGGG GAAAGAAGTC CGATTCTTGC GGCCTAAGGA CCCCACACGG 
CCAAGCTCCT TCCAGCAATG TCAGGGGCTG AGACATGTTG TCTTCACAGC AGAGACACAC 
AACTTCCCTA CAGGAGTAGC CCCCTTCAGT GGTGCAACCA CTGGCACAGG GGGCCGGATC 
CGAGATGTCC AGTGCACAGG CCGAGGGGCC CACGTGGTGG CTGGCACTGC TGGCTATTGC 
TTTGGAAACC TGCACATCCC AGGTTACAGT ATGCCCTGGG AGGATCCAAG CTTCCAGTAT 
CCAGGAAACT TTGCCCGACC CCTGGAGATT GCCATTGAGG CCAGTAATGG GGCTTCTGAC 
TACGGCAACA AGTTTGGGGA ACCAGTGCTT GCTGGCTTTG CCCGCTCCTT GGGCCTTCAG 
CTCCCGGACG GCCAGCGGCG TGAGTGGATC AAGCCCATCA TGTTTAGTGG AGGCATTGGG 
TCCATGGAAG CTGATCATGT GAGCAAGGAG CCCCCAGAGA CAGGCATGGA TGTCGTGAAG 
ATTGGCGGTC CTGTCTACAG GATTGGAGTT GGGGGCGGAG CTGCTTCGTC TGTGCAGGTG 
CAAGGAGACA ACGCCAGTGA CCTGGATTTT GGGGCTGTGC AGCGGGGAGA CCCAGAAATG 
GAGCAGAAGA TGAACCGTGT GATCCGGGCT TGTGTGGAGG CCCCTGGGGG AAACCCCATC 
TGCAGCCTCC ATGACCAGGG TGCTGGTGGC AATGGCAATG TCCTGAAGGA GCTGAGTGAC 
CCGGCTGGAG CCATCATTCA CACCAGCTGC TTCCAGCTGG GGGACCCAAC CCTGAATGCC 
CTGGAAATCT GGGGGGCCGA GTACCAGGAG TCGAACGCAC TTCTGCTGAG GCCCTCTGAC 
CGGGACTTCC TGAGTTGTGT CAGCGCCCGG GAACGCTGCC CAGTCTGCTT TGTGGGCACC 
ATCACTGGAG ACAGGAGAAT AGTGCTGGTG GATGATCGGG AGTGGCCTAT GGGCACAGGT 
GGCCAGGTGG ATGCGCCCCC CACTCCTCCC CCAACCCCTG TGGACCTGGA CCTGGACTGG 
GTGCTGGGCA AGATGCCCCA GAAGGAGTTC TTCCTCCAGA GGAGTCTCCC CTTGCTGCAG 
CCTCTGACTC TGCCCCCAGG GCTGAGCGTG CGCCAGGCTC TGGCGCGGGT CCTCAGGCTG 
CCTGCCGTGG CCAGCAAGCG CTACCTCACC AACAAGGTGG ACCGTTCTGT GGGCGGCCTG 
GTGGCCCAGC AGCAGTGTGT GGGACCCCTG CAGACCCCTC TGGCAGATGT GGCGGTTGTG 
GCACTGAGTC ACCAGGAGCT TGTAGGGGCC GCCACAGCCC TGGGAGAGCA GCCAGTCAAG 
AGCCTGCTGG ACCCGAAGGT TGCCGCCCGG CTGGCTGTGG CCGAAGCCCT CACCAACCTG 
GTATTTGCTC TGGTCACCGA CCTCCAGGAT GTGAAATGCA GCGGGAACTG GATGTGGGCA 
GCCAAGCTCC CAGGGGAGGG TGCAGCTCTG GCTGACGCCT GTGAGGCTAT GGTGGCAGTG 
ATGGCGGCCC TGGGTGTAGC GGTAGACGGT GGCAAGGACT CCCTCAGCAT GGCTGCCCGG 
GTTGGCTCTG AAACCGTGCG GGCTCCTGGG TCACTGGTCA TCTCAGCCTA TGCTGTCTGT 
TCAGACATTA CAGCCACTGT GACCCCAGAC CTCAAGCGTC CTGGGGGGAG AGGCCACCTG 
CTCTACGTGC CTCTGAGTCC TGGGCAGCAC CGGCTGGGGG GCACAGCTCT GGCTCAGTGC 
TTCTCCCAGC TTGGCGAGCA GCCTCCCGAC CTGGATGTTC CTGAGAACTT GGTGCGAGCC 
TTCAGCATCA CCCAGGGGCT GCTGAAAGAC CGCCTCCTCT GCTCAGGCCA TGATGTCAGT 
GATGGAGGTC TCATCACTTG CCTGCTAGAG ATGGCCTTTG CTGGGAATTG TGGGATAGAG 
GTGGACATCC CCGCACCTGG GGTCGATGCC CTGCCTGTGC TGTTCGCCGA GGAGCCAGGC 
CTGGTGCTGG AGGTGCAGGA GCCGGACCTC GCCCAAGTGC TGATGCGTTA CCGGAATGCT 
GGCCTCCACT GCCTAGAGCT GGGTCTCACA GGCGACACGG GGCCCCACGC CATGGTCCGC 
GTGTCGGTGA ACAGGACGGT GGTTCTGGAG GAACCTGTTG GGCAGCTACG AGCCCTCTGG 
GAGGAAACCA GTTTCCAGTT GGACCGGCTA CAGGCAAAAC CACACTGTGT GGCCCAGGAA 
GAGCAAGGGC TGAGGGAGCG GGCAGGGCCC ACCTTCTGCC TGCCGCCTAC CTTCCCCAGA 
GCTTCCGTGC CTTGTGAGCC TGGTGGTCCT GCCCCCCGAG TCGCCATCAT TCGGGAAGAG 
GGCAGTAATG GAGACCGGGA GATGGCAGAT GCCTTCCACT TGGCTGGATT TGAGGTGTGG 
GATGTAACCA TGCAGGACCT GTGCTCTGGA GCCATCGGGC TGGACACATT CCGCGGTGTG 
GCCTTCGTAG GCGGCTTCAG CTACGCGGAC GTCCTGGGCT CTGCCAAAGG CTGGGCAGCT 
GCTGTGACCT TTAACCCGCA GGCCGGGGCT GAGATGAAGC GCTTCCGCCA GCGGCCAGAC 
ACCTTCAGCC TGGGCGTGTG TAACGGCTGT CAGCTGCTGG CCCTGCTGGG CTGGGTGGGA 
GGCAGTTCTA GTGAGGAGGT GGTGGAGATG TGCCAGGAGT CCTGGCCGGC CCGGCCCGGT 
CTCCTGCTAC GCCACAACCT GTCTGGGCGC TTTGAGTCGC GCTGGGCTAG TGTGCGCGTG 
GGGCCCGGGC CAGCCCTGAT GCTGCGAGGG ATGGAAGGCG CGGTGCTGCC CGTGTGGAGC 
GCTCACGGGG AAGGTTACAT GGCATTTTCT TCTCCGGAAC TCCAAGCCCA GATGGAGGCC 
AGGGGCTTAG CTCCACTGCA CTGGGCAGAT GATGATGGGA ACCCCACGGA ACAGTACCCC 
CTGAATCCCA ACGGCTCCCC AGGGGGTGTC GCTGGCATCT GCTCCCCCGA TGGCCGCCAC 
TTGGCCCTCA TGCCTCACCC GGAGCGGTCT GTGAGGCCTT GGCAGTGGGC CTGGCGACCT 
CCTCCATTTG ACACTCTAAC CACCTCCCCC TGGCTCCAGC TCTTCATCAA TGCCCGGAAT 
TGGACCCAGG AATGCAGCTG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005220416.4
CDS	35..4138
Translation	MRMTLICAPEFEELTDWEKCYHAQRTPAEMSSVLHFYVRPSGHERAASEYIQRKLQRELPELQGVKTEQCYNVNWTAESFPSNKEMKKLTWLFGCPLLLDDVAQESWLRPGPTDLLLEVGPRLNFSTPTSSNIVSVCQAAGLGAVDRVEPTRRYLLSFAHPPSADLEAIALATLHDRMTEQRFPQPIQSFSSGCIPAPLSGPIDVLAEGRSALEKANQELGLALDSWDLDFYTKRFQELQRNPSTVEVFDLAQSNSEHSRHWFFKGRLHVDGQELPHSLFESIMSTQASSNPNNVLKFCDNSSAIQGKEVRFLRPKDPTRPSSFQQCQGLRHVVFTAETHNFPTGVAPFSGATTGTGGRIRDVQCTGRGAHVVAGTAGYCFGNLHIPGYSMPWEDPSFQYPGNFARPLEIAIEASNGASDYGNKFGEPVLAGFARSLGLQLPDGQRREWIKPIMFSGGIGSMEADHVSKEPPETGMDVVKIGGPVYRIGVGGGAASSVQVQGDNASDLDFGAVQRGDPEMEQKMNRVIRACVEAPGGNPICSLHDQGAGGNGNVLKELSDPAGAIIHTSCFQLGDPTLNALEIWGAEYQESNALLLRPSDRDFLSCVSARERCPVCFVGTITGDRRIVLVDDREWPMGTGGQVDAPPTPPPTPVDLDLDWVLGKMPQKEFFLQRSLPLLQPLTLPPGLSVRQALARVLRLPAVASKRYLTNKVDRSVGGLVAQQQCVGPLQTPLADVAVVALSHQELVGAATALGEQPVKSLLDPKVAARLAVAEALTNLVFALVTDLQDVKCSGNWMWAAKLPGEGAALADACEAMVAVMAALGVAVDGGKDSLSMAARVGSETVRAPGSLVISAYAVCSDITATVTPDLKRPGGRGHLLYVPLSPGQHRLGGTALAQCFSQLGEQPPDLDVPENLVRAFSITQGLLKDRLLCSGHDVSDGGLITCLLEMAFAGNCGIEVDIPAPGVDALPVLFAEEPGLVLEVQEPDLAQVLMRYRNAGLHCLELGLTGDTGPHAMVRVSVNRTVVLEEPVGQLRALWEETSFQLDRLQAKPHCVAQEEQGLRERAGPTFCLPPTFPRASVPCEPGGPAPRVAIIREEGSNGDREMADAFHLAGFEVWDVTMQDLCSGAIGLDTFRGVAFVGGFSYADVLGSAKGWAAAVTFNPQAGAEMKRFRQRPDTFSLGVCNGCQLLALLGWVGGSSSEEVVEMCQESWPARPGLLLRHNLSGRFESRWASVRVGPGPALMLRGMEGAVLPVWSAHGEGYMAFSSPELQAQMEARGLAPLHWADDDGNPTEQYPLNPNGSPGGVAGICSPDGRHLALMPHPERSVRPWQWAWRPPPFDTLTTSPWLQLFINARNWTQECSC

Target ORF information:

RefSeq Version	XM_005220359.4
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005220359.4

ORF Insert Sequence:

ATGAGGATGA CGTTGATCTG CGCGCCAGAA TTCGAGGAGC TGACGGACTG GGAGAAGTGC 
TACCACGCGC AACGGACACC TGCAGAGATG TCCTCCGTCC TTCACTTCTA TGTCCGCCCC 
TCTGGCCACG AGAGGGCAGC CTCTGAATAC ATTCAAAGGA AGCTGCAAAG GGAACTGCCA 
GAGCTGCAGG GTGTCAAGAC CGAGCAGTGC TACAATGTGA ACTGGACAGC GGAGTCCTTC 
CCGAGCAACA AGGAGATGAA GAAGCTGACA TGGCTATTTG GCTGCCCCTT GTTACTGGAC 
GATGTTGCTC AGGAATCCTG GCTTCGTCCT GGCCCCACAG ACCTGCTGCT GGAGGTCGGA 
CCCAGGCTGA ACTTTTCCAC CCCGACATCC AGCAACATTG TGTCAGTGTG CCAGGCTGCA 
GGGCTGGGGG CTGTGGACCG CGTGGAGCCA ACCCGGCGCT ACCTGCTCTC GTTTGCCCAC 
CCACCTTCAG CTGACCTGGA GGCCATTGCC CTGGCTACCC TGCATGACCG GATGACAGAG 
CAGCGCTTCC CCCAGCCCAT CCAGAGCTTC TCTTCAGGGT GCATCCCAGC ACCCCTGAGC 
GGCCCAATTG ACGTGCTGGC TGAGGGCCGG TCTGCCCTGG AGAAAGCCAA CCAGGAGCTA 
GGTCTGGCCC TAGACTCCTG GGACCTGGAT TTCTACACCA AGCGCTTCCA GGAGCTGCAG 
CGCAACCCCA GCACTGTGGA AGTCTTCGAC TTGGCTCAGT CCAATAGCGA ACACAGCCGA 
CACTGGTTCT TCAAGGGTCG ACTCCATGTG GATGGACAGG AGCTGCCACA CTCGCTGTTT 
GAGTCCATCA TGAGCACCCA GGCGTCCTCC AACCCTAACA ACGTCCTCAA GTTCTGTGAC 
AACAGCAGTG CCATCCAGGG GAAAGAAGTC CGATTCTTGC GGCCTAAGGA CCCCACACGG 
CCAAGCTCCT TCCAGCAATG TCAGGGGCTG AGACATGTTG TCTTCACAGC AGAGACACAC 
AACTTCCCTA CAGGAGTAGC CCCCTTCAGT GGTGCAACCA CTGGCACAGG GGGCCGGATC 
CGAGATGTCC AGTGCACAGG CCGAGGGGCC CACGTGGTGG CTGGCACTGC TGGCTATTGC 
TTTGGAAACC TGCACATCCC AGGTTACAGT ATGCCCTGGG AGGATCCAAG CTTCCAGTAT 
CCAGGAAACT TTGCCCGACC CCTGGAGATT GCCATTGAGG CCAGTAATGG GGCTTCTGAC 
TACGGCAACA AGTTTGGGGA ACCAGTGCTT GCTGGCTTTG CCCGCTCCTT GGGCCTTCAG 
CTCCCGGACG GCCAGCGGCG TGAGTGGATC AAGCCCATCA TGTTTAGTGG AGGCATTGGG 
TCCATGGAAG CTGATCATGT GAGCAAGGAG CCCCCAGAGA CAGGCATGGA TGTCGTGAAG 
ATTGGCGGTC CTGTCTACAG GATTGGAGTT GGGGGCGGAG CTGCTTCGTC TGTGCAGGTG 
CAAGGAGACA ACGCCAGTGA CCTGGATTTT GGGGCTGTGC AGCGGGGAGA CCCAGAAATG 
GAGCAGAAGA TGAACCGTGT GATCCGGGCT TGTGTGGAGG CCCCTGGGGG AAACCCCATC 
TGCAGCCTCC ATGACCAGGG TGCTGGTGGC AATGGCAATG TCCTGAAGGA GCTGAGTGAC 
CCGGCTGGAG CCATCATTCA CACCAGCTGC TTCCAGCTGG GGGACCCAAC CCTGAATGCC 
CTGGAAATCT GGGGGGCCGA GTACCAGGAG TCGAACGCAC TTCTGCTGAG GCCCTCTGAC 
CGGGACTTCC TGAGTTGTGT CAGCGCCCGG GAACGCTGCC CAGTCTGCTT TGTGGGCACC 
ATCACTGGAG ACAGGAGAAT AGTGCTGGTG GATGATCGGG AGTGGCCTAT GGGCACAGGT 
GGCCAGGTGG ATGCGCCCCC CACTCCTCCC CCAACCCCTG TGGACCTGGA CCTGGACTGG 
GTGCTGGGCA AGATGCCCCA GAAGGAGTTC TTCCTCCAGA GGAGTCTCCC CTTGCTGCAG 
CCTCTGACTC TGCCCCCAGG GCTGAGCGTG CGCCAGGCTC TGGCGCGGGT CCTCAGGCTG 
CCTGCCGTGG CCAGCAAGCG CTACCTCACC AACAAGGTGG ACCGTTCTGT GGGCGGCCTG 
GTGGCCCAGC AGCAGTGTGT GGGACCCCTG CAGACCCCTC TGGCAGATGT GGCGGTTGTG 
GCACTGAGTC ACCAGGAGCT TGTAGGGGCC GCCACAGCCC TGGGAGAGCA GCCAGTCAAG 
AGCCTGCTGG ACCCGAAGGT TGCCGCCCGG CTGGCTGTGG CCGAAGCCCT CACCAACCTG 
GTATTTGCTC TGGTCACCGA CCTCCAGGAT GTGAAATGCA GCGGGAACTG GATGTGGGCA 
GCCAAGCTCC CAGGGGAGGG TGCAGCTCTG GCTGACGCCT GTGAGGCTAT GGTGGCAGTG 
ATGGCGGCCC TGGGTGTAGC GGTAGACGGT GGCAAGGACT CCCTCAGCAT GGCTGCCCGG 
GTTGGCTCTG AAACCGTGCG GGCTCCTGGG TCACTGGTCA TCTCAGCCTA TGCTGTCTGT 
TCAGACATTA CAGCCACTGT GACCCCAGAC CTCAAGCGTC CTGGGGGGAG AGGCCACCTG 
CTCTACGTGC CTCTGAGTCC TGGGCAGCAC CGGCTGGGGG GCACAGCTCT GGCTCAGTGC 
TTCTCCCAGC TTGGCGAGCA GCCTCCCGAC CTGGATGTTC CTGAGAACTT GGTGCGAGCC 
TTCAGCATCA CCCAGGGGCT GCTGAAAGAC CGCCTCCTCT GCTCAGGCCA TGATGTCAGT 
GATGGAGGTC TCATCACTTG CCTGCTAGAG ATGGCCTTTG CTGGGAATTG TGGGATAGAG 
GTGGACATCC CCGCACCTGG GGTCGATGCC CTGCCTGTGC TGTTCGCCGA GGAGCCAGGC 
CTGGTGCTGG AGGTGCAGGA GCCGGACCTC GCCCAAGTGC TGATGCGTTA CCGGAATGCT 
GGCCTCCACT GCCTAGAGCT GGGTCTCACA GGCGACACGG GGCCCCACGC CATGGTCCGC 
GTGTCGGTGA ACAGGACGGT GGTTCTGGAG GAACCTGTTG GGCAGCTACG AGCCCTCTGG 
GAGGAAACCA GTTTCCAGTT GGACCGGCTA CAGGCAAAAC CACACTGTGT GGCCCAGGAA 
GAGCAAGGGC TGAGGGAGCG GGCAGGGCCC ACCTTCTGCC TGCCGCCTAC CTTCCCCAGA 
GCTTCCGTGC CTTGTGAGCC TGGTGGTCCT GCCCCCCGAG TCGCCATCAT TCGGGAAGAG 
GGCAGTAATG GAGACCGGGA GATGGCAGAT GCCTTCCACT TGGCTGGATT TGAGGTGTGG 
GATGTAACCA TGCAGGACCT GTGCTCTGGA GCCATCGGGC TGGACACATT CCGCGGTGTG 
GCCTTCGTAG GCGGCTTCAG CTACGCGGAC GTCCTGGGCT CTGCCAAAGG CTGGGCAGCT 
GCTGTGACCT TTAACCCGCA GGCCGGGGCT GAGATGAAGC GCTTCCGCCA GCGGCCAGAC 
ACCTTCAGCC TGGGCGTGTG TAACGGCTGT CAGCTGCTGG CCCTGCTGGG CTGGGTGGGA 
GGCAGTTCTA GTGAGGAGGT GGTGGAGATG TGCCAGGAGT CCTGGCCGGC CCGGCCCGGT 
CTCCTGCTAC GCCACAACCT GTCTGGGCGC TTTGAGTCGC GCTGGGCTAG TGTGCGCGTG 
GGGCCCGGGC CAGCCCTGAT GCTGCGAGGG ATGGAAGGCG CGGTGCTGCC CGTGTGGAGC 
GCTCACGGGG AAGGTTACAT GGCATTTTCT TCTCCGGAAC TCCAAGCCCA GATGGAGGCC 
AGGGGCTTAG CTCCACTGCA CTGGGCAGAT GATGATGGGA ACCCCACGGA ACAGTACCCC 
CTGAATCCCA ACGGCTCCCC AGGGGGTGTC GCTGGCATCT GCTCCCCCGA TGGCCGCCAC 
TTGGCCCTCA TGCCTCACCC GGAGCGGTCT GTGAGGCCTT GGCAGTGGGC CTGGCGACCT 
CCTCCATTTG ACACTCTAAC CACCTCCCCC TGGCTCCAGC TCTTCATCAA TGCCCGGAAT 
TGGACCCAGG AATGCAGCTG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFAS ( XM_015458726.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015314212.1 Bos taurus phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa93869
Clone ID Related Accession (Same CDS sequence)	XM_015458726.1
Accession Version	XM_015458726.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	phosphoribosylformylglycinamidine synthase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104494.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTCCG TCCTTCACTT CTATGTCCGC CCCTCTGGCC ACGAGAGGGC AGCCTCTGAA 
TACATTCAAA GGAAGCTGCA AAGGGAACTG CCAGAGCTGC AGGGTGTCAA GACCGAGCAG 
TGCTACAATG TGAACTGGGC AGCGGAGTCC TTCCCGAGCA ACAAGGAGAT GAAGAAGCTG 
ACATGGCTAT TTGGCTGCCC CTTGTTACTG GACGATGTTG CTCAGGAATC CTGGCTTCGT 
CCTGGCCCCA CAGACCTGCT GCTGGAGGTC GGACCCAGGC TGAACTTTTC CACCCCGACA 
TCCAGCAACA TTGTGTCAGT GTGCCAGGCT GCAGGGCTGG GGGCTGTGGA CCGCGTGGAG 
CCAACCCGGC GCTACCTGCT CTCGTTTGCC CACCCACCTT CAGCTGACCT GGAGGCCATT 
GCCCTGGCTA CCCTGCATGA CCGGATGACA GAGCAGCACT TCCCCCAGCC CATCCAGAGC 
TTCTCTTCAG GGTGCATCCC AGCACCCCTG AGCGGCCCAA TTGACGTGCT GGCTGAGGGC 
CGGTCTGCCC TGGAGAAAGC CAACCAGGAG CTAGGTCTGG CCCTAGACTC CTGGGACCTG 
GATTTCTACA CCAAGCGCTT CCAGGAGCTG CAGCGCAACC CCAGCACTGT GGAAGTCTTC 
GACTTGGCTC AGTCCAATAG CGAACACAGC CGACACTGGT TCTTCAAGGG TCGACTCCAT 
GTGGATGGAC AGGAGCTGCC ACACTCGCTG TTTGAGTCCA TCATGAGCAC CCAGGCGTCC 
TCCAACCCTA ACAACGTCCT CAAGTTCTGT GACAACAGCA GTGCCATCCA GGGGAAAGAA 
GTCCGATTCT TGCGGCCTAA GGACCCCACA CGGCCAAGCT CCTTCCAGCA ATGTCAGGGG 
CTGAGACATG TTGTCTTCAC AGCAGAGACA CACAACTTCC CTACAGGAGT AGCCCCCTTC 
AGTGGTGCAG CCACTGGCAC AGGGGGCCGG ATCCGAGATG TCCAGTGCAC AGGCCGAGGG 
GCCCACGTGG TGGCTGGCAC TGCTGGCTAT TGCTTTGGAA ACCTGCACAT CCCAGGTTAC 
AGTATGCCCT GGGAGGATCC AAGCTTCCAG TATCCAGGAA ACTTTGCCCG ACCCCTGGAG 
ATTGCCATTG AGGCCAGTAA TGGGGCTTCT GACTACGGCA ACAAGTTTGG GGAACCAGTG 
CTTGCTGGCT TTGCCCGCTC CTTGGGCCTT CAGCTTCCGG ACGGCCAGCG GCGTGAGTGG 
ATCAAGCCCA TAATGTTTAG TGGAGGCATT GGGTCCATGG AAGCTGAGCA TGTGAGCAAG 
GAGCCCCCAG AGACAGGCAT GGATGTCGTG AAGATTGGCG GTCCTGTCTA CAGGATTGGA 
GTTGGGGGCG GAGCTGCTTC GTCTGTGCAG GTGCAAGGAG ACAACGCCAG TGACCTGGAT 
TTTGGGGCTG TGCAGCGGGG AGACCCAGAG ATGGAGCAGA AGATGAACCG TGTGATCCGG 
GCTTGTGTGG AGGCCCCTGG GGGAAACCCC ATCTGCAGCC TCCATGACCA GGGTGCTGGT 
GGCAATGGCA ATGTCCTGAA GGAGCTGAGT GACCCGGCTG GAGCCGTCAT TCACACCAGC 
TGCTTCCAGC TGGGGGACCC AACCCTGAAT GCCCTGGAAA TCTGGGGGGC CGAGTACCAG 
GAGTCGAACG CACTTCTGCT GAGGCCCTCT GACCGGGACT TCCTGAGTTG TGTCAGCGCC 
CGGGAACGCT GCCCAGTCTG CTTTGTGGGC ACCATCACTG GAGACAGGAG AATAGTGCTG 
GTGGATGATC GGGAGTGGCC TATGGGCACA GGTGGCCAGG TGGATGCGCC CCCCACTCCT 
CCCCCAACCC CTGTGGACCT GGACCTGGAC TGGGTGCTGG GCAAGATGCC CCAGAAGGAG 
TTCTTCCTCC AGAGGAGTCT CCCCTTGCTG CAGCCTCTGA CTCTGCCCCC AGGGCTGAGC 
GTGCGCCAGG CTCTGGCGCG GGTCCTCAGG CTGCCTGCCG TGGCCAGCAA GCGCTACCTC 
ACCAACAAGG TGGACCGTTC TGTGGGCGGC CTGGTGGCCC AGCAGCAGTG TGTGGGACCC 
CTGCAGACCC CTCTGGCAGA TGTGGCGGTT GTGGCACTGA GTCACCAGGA GCTTGTAGGG 
GCCGCCACAG CCCTGGGAGA GCAGCCAGTC AAGAGCCTGC TGGACCCGAA GGTTGCCGCC 
CGGCTGGCTG TGGCCGAAGC CCTCACCAAC CTGGTATTTG CTCTGGTCAC CGACCTCCAG 
GATGTGAAAT GCAGCGGGAA CTGGATGTGG GCAGCCAAGC TCCCAGGGGA GGGTGCAGCT 
CTGGCTGACG CCTGTGAGGC TATGGTGGCA GTGATGGCGG CCCTGGGTGT AGCGGTAGAC 
GGTGGCAAGG ACTCCCTCAG CATGGCTGCC CGGGTTGGCT CTGAAACCGT GCGGGCTCCT 
GGGTCACTGG TCATCTCAGC CTATGCTGTC TGTTCAGACA TTACAGCCAC TGTGACCCCA 
GACCTCAAGC GTCCTGGGGG GAGAGGCCAC CTGCTCTACG TGCCTCTGAG TCCTGGGCAG 
CACCGGCTGG GGGGCACAGC TCTGGCTCAG TGCTTCTCCC AGCTTGGCGA GCAGCCTCCC 
GACCTGGATG TTCCTGAGAA CTTGGTGCGA GCCTTCAGCA TCACCCAGGG GCTGCTGAAA 
GACCGCCTCC TCTGCTCAGG CCATGATGTC AGTGATGGAG GTCTCATCAC TTGCCTGCTA 
GAGATGGCCT TTGCTGGGAA TTGTGGGATA GAGGTGGACA TCCCCGCACC TGGGGTCGAT 
GCCCTGCCTG TGCTGTTCGC CGAGGAGCCA GGCCTGGTGC TGGAGGTGCA GGAGCCGGAC 
CTCGCCCAAG TGCTGATGCG TTACCGGAAT GCTGGCCTCC ACTGCCTAGA GCTGGGTCTC 
ACAGGCGACA CGGGGCCCCA CGCCATGGTC CGCGTGTCGG TGAACAGGAC GGTGGTTCTG 
GAGGAACCTG TTGGGCAGCT ACGAGCCCTC TGGGAGGAAA CCAGTTTCCA GTTGGACCGG 
CTACAGGCAA AACCACACTG TGTGGCCCAG GAAGAGCAAG GGCTGAGGGA GCGGGCAGGG 
CCCACCTTCT GCCTGCCGCC TACCTTCCCC AGAGCTTCCG TGCCTTGTGG TCCTGCCCCC 
CGAGTCGCCA TCATTCGGGA AGAGGGCAGT AATGGAGACC GGGAGATGGC AGATGCCTTC 
CACTTGGCTG GATTTGAGGT GTGGGATGTA ACCATGCAGG ACCTGTGCTC TGGAGCCATC 
GGGCTGGACA CATTCCGCGG TGTGGCCTTC GTAGGCGGCT TCAGCTACGC GGACGTCCTG 
GGCTCTGCCA AAGGCTGGGC AGCTGCTGTG ACCTTTAACC CGCAGGCCGG GGCTGAGATG 
AAGCGCTTCC GCCAGCGGCC AGACACCTTC AGCCTGGGCG TGTGTAACGG CTGTCAGCTG 
CTGGCCCTGC TGGGCTGGGT GGGAGGCAGT TCTAGTGAGG AGGTGGTGGA GATGTGCCAG 
GAGTCCTGGC CGGCCCGGCC CGGTCTCCTG CTACGCCACA ACCTGTCTGG GCGCTTTGAG 
TCGCGCTGGG CTAGTGTGCG CGTGGGGCCC GGGCCAGCCC TGATGCTGCG AGGGATGGAA 
GGCGCGGTGC TGCCCGTGTG GAGCGCTCAC GGGGAAGGTT ACATGGCATT TTCTTCTCCG 
GAACTCCAAG CCCAGATGGA GGCCAGGGGC TTAGCTCCAC TGCACTGGGC AGATGATGAT 
GGGAACCCCA CGGAACAGTA CCCCCTGAAT CCCAACGGCT CCCCAGGGGG TGTCGCTGGC 
ATCTGCTCCC CCGATGGCCG CCACTTGGCC CTCATGCCTC ACCCGGAGCG GTCTGTGAGG 
CCTTGGCAGT GGGCCTGGCG ACCTCCTCCA TTTGACACTC TAACCACCTC CCCCTGGCTC 
CAGCTCTTCA TCAATGCCCG GAATTGGACC CAGGAATGCA GCTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015314212.1
CDS	40..4047
Translation	MSSVLHFYVRPSGHERAASEYIQRKLQRELPELQGVKTEQCYNVNWAAESFPSNKEMKKLTWLFGCPLLLDDVAQESWLRPGPTDLLLEVGPRLNFSTPTSSNIVSVCQAAGLGAVDRVEPTRRYLLSFAHPPSADLEAIALATLHDRMTEQHFPQPIQSFSSGCIPAPLSGPIDVLAEGRSALEKANQELGLALDSWDLDFYTKRFQELQRNPSTVEVFDLAQSNSEHSRHWFFKGRLHVDGQELPHSLFESIMSTQASSNPNNVLKFCDNSSAIQGKEVRFLRPKDPTRPSSFQQCQGLRHVVFTAETHNFPTGVAPFSGAATGTGGRIRDVQCTGRGAHVVAGTAGYCFGNLHIPGYSMPWEDPSFQYPGNFARPLEIAIEASNGASDYGNKFGEPVLAGFARSLGLQLPDGQRREWIKPIMFSGGIGSMEAEHVSKEPPETGMDVVKIGGPVYRIGVGGGAASSVQVQGDNASDLDFGAVQRGDPEMEQKMNRVIRACVEAPGGNPICSLHDQGAGGNGNVLKELSDPAGAVIHTSCFQLGDPTLNALEIWGAEYQESNALLLRPSDRDFLSCVSARERCPVCFVGTITGDRRIVLVDDREWPMGTGGQVDAPPTPPPTPVDLDLDWVLGKMPQKEFFLQRSLPLLQPLTLPPGLSVRQALARVLRLPAVASKRYLTNKVDRSVGGLVAQQQCVGPLQTPLADVAVVALSHQELVGAATALGEQPVKSLLDPKVAARLAVAEALTNLVFALVTDLQDVKCSGNWMWAAKLPGEGAALADACEAMVAVMAALGVAVDGGKDSLSMAARVGSETVRAPGSLVISAYAVCSDITATVTPDLKRPGGRGHLLYVPLSPGQHRLGGTALAQCFSQLGEQPPDLDVPENLVRAFSITQGLLKDRLLCSGHDVSDGGLITCLLEMAFAGNCGIEVDIPAPGVDALPVLFAEEPGLVLEVQEPDLAQVLMRYRNAGLHCLELGLTGDTGPHAMVRVSVNRTVVLEEPVGQLRALWEETSFQLDRLQAKPHCVAQEEQGLRERAGPTFCLPPTFPRASVPCGPAPRVAIIREEGSNGDREMADAFHLAGFEVWDVTMQDLCSGAIGLDTFRGVAFVGGFSYADVLGSAKGWAAAVTFNPQAGAEMKRFRQRPDTFSLGVCNGCQLLALLGWVGGSSSEEVVEMCQESWPARPGLLLRHNLSGRFESRWASVRVGPGPALMLRGMEGAVLPVWSAHGEGYMAFSSPELQAQMEARGLAPLHWADDDGNPTEQYPLNPNGSPGGVAGICSPDGRHLALMPHPERSVRPWQWAWRPPPFDTLTTSPWLQLFINARNWTQECSC

Target ORF information:

RefSeq Version	XM_015458726.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015458726.1

ORF Insert Sequence:

ATGTCCTCCG TCCTTCACTT CTATGTCCGC CCCTCTGGCC ACGAGAGGGC AGCCTCTGAA 
TACATTCAAA GGAAGCTGCA AAGGGAACTG CCAGAGCTGC AGGGTGTCAA GACCGAGCAG 
TGCTACAATG TGAACTGGGC AGCGGAGTCC TTCCCGAGCA ACAAGGAGAT GAAGAAGCTG 
ACATGGCTAT TTGGCTGCCC CTTGTTACTG GACGATGTTG CTCAGGAATC CTGGCTTCGT 
CCTGGCCCCA CAGACCTGCT GCTGGAGGTC GGACCCAGGC TGAACTTTTC CACCCCGACA 
TCCAGCAACA TTGTGTCAGT GTGCCAGGCT GCAGGGCTGG GGGCTGTGGA CCGCGTGGAG 
CCAACCCGGC GCTACCTGCT CTCGTTTGCC CACCCACCTT CAGCTGACCT GGAGGCCATT 
GCCCTGGCTA CCCTGCATGA CCGGATGACA GAGCAGCACT TCCCCCAGCC CATCCAGAGC 
TTCTCTTCAG GGTGCATCCC AGCACCCCTG AGCGGCCCAA TTGACGTGCT GGCTGAGGGC 
CGGTCTGCCC TGGAGAAAGC CAACCAGGAG CTAGGTCTGG CCCTAGACTC CTGGGACCTG 
GATTTCTACA CCAAGCGCTT CCAGGAGCTG CAGCGCAACC CCAGCACTGT GGAAGTCTTC 
GACTTGGCTC AGTCCAATAG CGAACACAGC CGACACTGGT TCTTCAAGGG TCGACTCCAT 
GTGGATGGAC AGGAGCTGCC ACACTCGCTG TTTGAGTCCA TCATGAGCAC CCAGGCGTCC 
TCCAACCCTA ACAACGTCCT CAAGTTCTGT GACAACAGCA GTGCCATCCA GGGGAAAGAA 
GTCCGATTCT TGCGGCCTAA GGACCCCACA CGGCCAAGCT CCTTCCAGCA ATGTCAGGGG 
CTGAGACATG TTGTCTTCAC AGCAGAGACA CACAACTTCC CTACAGGAGT AGCCCCCTTC 
AGTGGTGCAG CCACTGGCAC AGGGGGCCGG ATCCGAGATG TCCAGTGCAC AGGCCGAGGG 
GCCCACGTGG TGGCTGGCAC TGCTGGCTAT TGCTTTGGAA ACCTGCACAT CCCAGGTTAC 
AGTATGCCCT GGGAGGATCC AAGCTTCCAG TATCCAGGAA ACTTTGCCCG ACCCCTGGAG 
ATTGCCATTG AGGCCAGTAA TGGGGCTTCT GACTACGGCA ACAAGTTTGG GGAACCAGTG 
CTTGCTGGCT TTGCCCGCTC CTTGGGCCTT CAGCTTCCGG ACGGCCAGCG GCGTGAGTGG 
ATCAAGCCCA TAATGTTTAG TGGAGGCATT GGGTCCATGG AAGCTGAGCA TGTGAGCAAG 
GAGCCCCCAG AGACAGGCAT GGATGTCGTG AAGATTGGCG GTCCTGTCTA CAGGATTGGA 
GTTGGGGGCG GAGCTGCTTC GTCTGTGCAG GTGCAAGGAG ACAACGCCAG TGACCTGGAT 
TTTGGGGCTG TGCAGCGGGG AGACCCAGAG ATGGAGCAGA AGATGAACCG TGTGATCCGG 
GCTTGTGTGG AGGCCCCTGG GGGAAACCCC ATCTGCAGCC TCCATGACCA GGGTGCTGGT 
GGCAATGGCA ATGTCCTGAA GGAGCTGAGT GACCCGGCTG GAGCCGTCAT TCACACCAGC 
TGCTTCCAGC TGGGGGACCC AACCCTGAAT GCCCTGGAAA TCTGGGGGGC CGAGTACCAG 
GAGTCGAACG CACTTCTGCT GAGGCCCTCT GACCGGGACT TCCTGAGTTG TGTCAGCGCC 
CGGGAACGCT GCCCAGTCTG CTTTGTGGGC ACCATCACTG GAGACAGGAG AATAGTGCTG 
GTGGATGATC GGGAGTGGCC TATGGGCACA GGTGGCCAGG TGGATGCGCC CCCCACTCCT 
CCCCCAACCC CTGTGGACCT GGACCTGGAC TGGGTGCTGG GCAAGATGCC CCAGAAGGAG 
TTCTTCCTCC AGAGGAGTCT CCCCTTGCTG CAGCCTCTGA CTCTGCCCCC AGGGCTGAGC 
GTGCGCCAGG CTCTGGCGCG GGTCCTCAGG CTGCCTGCCG TGGCCAGCAA GCGCTACCTC 
ACCAACAAGG TGGACCGTTC TGTGGGCGGC CTGGTGGCCC AGCAGCAGTG TGTGGGACCC 
CTGCAGACCC CTCTGGCAGA TGTGGCGGTT GTGGCACTGA GTCACCAGGA GCTTGTAGGG 
GCCGCCACAG CCCTGGGAGA GCAGCCAGTC AAGAGCCTGC TGGACCCGAA GGTTGCCGCC 
CGGCTGGCTG TGGCCGAAGC CCTCACCAAC CTGGTATTTG CTCTGGTCAC CGACCTCCAG 
GATGTGAAAT GCAGCGGGAA CTGGATGTGG GCAGCCAAGC TCCCAGGGGA GGGTGCAGCT 
CTGGCTGACG CCTGTGAGGC TATGGTGGCA GTGATGGCGG CCCTGGGTGT AGCGGTAGAC 
GGTGGCAAGG ACTCCCTCAG CATGGCTGCC CGGGTTGGCT CTGAAACCGT GCGGGCTCCT 
GGGTCACTGG TCATCTCAGC CTATGCTGTC TGTTCAGACA TTACAGCCAC TGTGACCCCA 
GACCTCAAGC GTCCTGGGGG GAGAGGCCAC CTGCTCTACG TGCCTCTGAG TCCTGGGCAG 
CACCGGCTGG GGGGCACAGC TCTGGCTCAG TGCTTCTCCC AGCTTGGCGA GCAGCCTCCC 
GACCTGGATG TTCCTGAGAA CTTGGTGCGA GCCTTCAGCA TCACCCAGGG GCTGCTGAAA 
GACCGCCTCC TCTGCTCAGG CCATGATGTC AGTGATGGAG GTCTCATCAC TTGCCTGCTA 
GAGATGGCCT TTGCTGGGAA TTGTGGGATA GAGGTGGACA TCCCCGCACC TGGGGTCGAT 
GCCCTGCCTG TGCTGTTCGC CGAGGAGCCA GGCCTGGTGC TGGAGGTGCA GGAGCCGGAC 
CTCGCCCAAG TGCTGATGCG TTACCGGAAT GCTGGCCTCC ACTGCCTAGA GCTGGGTCTC 
ACAGGCGACA CGGGGCCCCA CGCCATGGTC CGCGTGTCGG TGAACAGGAC GGTGGTTCTG 
GAGGAACCTG TTGGGCAGCT ACGAGCCCTC TGGGAGGAAA CCAGTTTCCA GTTGGACCGG 
CTACAGGCAA AACCACACTG TGTGGCCCAG GAAGAGCAAG GGCTGAGGGA GCGGGCAGGG 
CCCACCTTCT GCCTGCCGCC TACCTTCCCC AGAGCTTCCG TGCCTTGTGG TCCTGCCCCC 
CGAGTCGCCA TCATTCGGGA AGAGGGCAGT AATGGAGACC GGGAGATGGC AGATGCCTTC 
CACTTGGCTG GATTTGAGGT GTGGGATGTA ACCATGCAGG ACCTGTGCTC TGGAGCCATC 
GGGCTGGACA CATTCCGCGG TGTGGCCTTC GTAGGCGGCT TCAGCTACGC GGACGTCCTG 
GGCTCTGCCA AAGGCTGGGC AGCTGCTGTG ACCTTTAACC CGCAGGCCGG GGCTGAGATG 
AAGCGCTTCC GCCAGCGGCC AGACACCTTC AGCCTGGGCG TGTGTAACGG CTGTCAGCTG 
CTGGCCCTGC TGGGCTGGGT GGGAGGCAGT TCTAGTGAGG AGGTGGTGGA GATGTGCCAG 
GAGTCCTGGC CGGCCCGGCC CGGTCTCCTG CTACGCCACA ACCTGTCTGG GCGCTTTGAG 
TCGCGCTGGG CTAGTGTGCG CGTGGGGCCC GGGCCAGCCC TGATGCTGCG AGGGATGGAA 
GGCGCGGTGC TGCCCGTGTG GAGCGCTCAC GGGGAAGGTT ACATGGCATT TTCTTCTCCG 
GAACTCCAAG CCCAGATGGA GGCCAGGGGC TTAGCTCCAC TGCACTGGGC AGATGATGAT 
GGGAACCCCA CGGAACAGTA CCCCCTGAAT CCCAACGGCT CCCCAGGGGG TGTCGCTGGC 
ATCTGCTCCC CCGATGGCCG CCACTTGGCC CTCATGCCTC ACCCGGAGCG GTCTGTGAGG 
CCTTGGCAGT GGGCCTGGCG ACCTCCTCCA TTTGACACTC TAACCACCTC CCCCTGGCTC 
CAGCTCTTCA TCAATGCCCG GAATTGGACC CAGGAATGCA GCTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFAS ( XM_005220359.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005220416.3 Bos taurus phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa93870
Clone ID Related Accession (Same CDS sequence)	XM_005220359.3
Accession Version	XM_005220359.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4104bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	phosphoribosylformylglycinamidine synthase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104494.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005220359.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGATGA CGTTGATCTG CGCGCCAGAA TTCGAGGAGC TGACGGACTG GGAGAAGTGC 
TACCACGCGC AACGGACACC TGCAGAGATG TCCTCCGTCC TTCACTTCTA TGTCCGCCCC 
TCTGGCCACG AGAGGGCAGC CTCTGAATAC ATTCAAAGGA AGCTGCAAAG GGAACTGCCA 
GAGCTGCAGG GTGTCAAGAC CGAGCAGTGC TACAATGTGA ACTGGGCAGC GGAGTCCTTC 
CCGAGCAACA AGGAGATGAA GAAGCTGACA TGGCTATTTG GCTGCCCCTT GTTACTGGAC 
GATGTTGCTC AGGAATCCTG GCTTCGTCCT GGCCCCACAG ACCTGCTGCT GGAGGTCGGA 
CCCAGGCTGA ACTTTTCCAC CCCGACATCC AGCAACATTG TGTCAGTGTG CCAGGCTGCA 
GGGCTGGGGG CTGTGGACCG CGTGGAGCCA ACCCGGCGCT ACCTGCTCTC GTTTGCCCAC 
CCACCTTCAG CTGACCTGGA GGCCATTGCC CTGGCTACCC TGCATGACCG GATGACAGAG 
CAGCACTTCC CCCAGCCCAT CCAGAGCTTC TCTTCAGGGT GCATCCCAGC ACCCCTGAGC 
GGCCCAATTG ACGTGCTGGC TGAGGGCCGG TCTGCCCTGG AGAAAGCCAA CCAGGAGCTA 
GGTCTGGCCC TAGACTCCTG GGACCTGGAT TTCTACACCA AGCGCTTCCA GGAGCTGCAG 
CGCAACCCCA GCACTGTGGA AGTCTTCGAC TTGGCTCAGT CCAATAGCGA ACACAGCCGA 
CACTGGTTCT TCAAGGGTCG ACTCCATGTG GATGGACAGG AGCTGCCACA CTCGCTGTTT 
GAGTCCATCA TGAGCACCCA GGCGTCCTCC AACCCTAACA ACGTCCTCAA GTTCTGTGAC 
AACAGCAGTG CCATCCAGGG GAAAGAAGTC CGATTCTTGC GGCCTAAGGA CCCCACACGG 
CCAAGCTCCT TCCAGCAATG TCAGGGGCTG AGACATGTTG TCTTCACAGC AGAGACACAC 
AACTTCCCTA CAGGAGTAGC CCCCTTCAGT GGTGCAGCCA CTGGCACAGG GGGCCGGATC 
CGAGATGTCC AGTGCACAGG CCGAGGGGCC CACGTGGTGG CTGGCACTGC TGGCTATTGC 
TTTGGAAACC TGCACATCCC AGGTTACAGT ATGCCCTGGG AGGATCCAAG CTTCCAGTAT 
CCAGGAAACT TTGCCCGACC CCTGGAGATT GCCATTGAGG CCAGTAATGG GGCTTCTGAC 
TACGGCAACA AGTTTGGGGA ACCAGTGCTT GCTGGCTTTG CCCGCTCCTT GGGCCTTCAG 
CTTCCGGACG GCCAGCGGCG TGAGTGGATC AAGCCCATAA TGTTTAGTGG AGGCATTGGG 
TCCATGGAAG CTGAGCATGT GAGCAAGGAG CCCCCAGAGA CAGGCATGGA TGTCGTGAAG 
ATTGGCGGTC CTGTCTACAG GATTGGAGTT GGGGGCGGAG CTGCTTCGTC TGTGCAGGTG 
CAAGGAGACA ACGCCAGTGA CCTGGATTTT GGGGCTGTGC AGCGGGGAGA CCCAGAGATG 
GAGCAGAAGA TGAACCGTGT GATCCGGGCT TGTGTGGAGG CCCCTGGGGG AAACCCCATC 
TGCAGCCTCC ATGACCAGGG TGCTGGTGGC AATGGCAATG TCCTGAAGGA GCTGAGTGAC 
CCGGCTGGAG CCGTCATTCA CACCAGCTGC TTCCAGCTGG GGGACCCAAC CCTGAATGCC 
CTGGAAATCT GGGGGGCCGA GTACCAGGAG TCGAACGCAC TTCTGCTGAG GCCCTCTGAC 
CGGGACTTCC TGAGTTGTGT CAGCGCCCGG GAACGCTGCC CAGTCTGCTT TGTGGGCACC 
ATCACTGGAG ACAGGAGAAT AGTGCTGGTG GATGATCGGG AGTGGCCTAT GGGCACAGGT 
GGCCAGGTGG ATGCGCCCCC CACTCCTCCC CCAACCCCTG TGGACCTGGA CCTGGACTGG 
GTGCTGGGCA AGATGCCCCA GAAGGAGTTC TTCCTCCAGA GGAGTCTCCC CTTGCTGCAG 
CCTCTGACTC TGCCCCCAGG GCTGAGCGTG CGCCAGGCTC TGGCGCGGGT CCTCAGGCTG 
CCTGCCGTGG CCAGCAAGCG CTACCTCACC AACAAGGTGG ACCGTTCTGT GGGCGGCCTG 
GTGGCCCAGC AGCAGTGTGT GGGACCCCTG CAGACCCCTC TGGCAGATGT GGCGGTTGTG 
GCACTGAGTC ACCAGGAGCT TGTAGGGGCC GCCACAGCCC TGGGAGAGCA GCCAGTCAAG 
AGCCTGCTGG ACCCGAAGGT TGCCGCCCGG CTGGCTGTGG CCGAAGCCCT CACCAACCTG 
GTATTTGCTC TGGTCACCGA CCTCCAGGAT GTGAAATGCA GCGGGAACTG GATGTGGGCA 
GCCAAGCTCC CAGGGGAGGG TGCAGCTCTG GCTGACGCCT GTGAGGCTAT GGTGGCAGTG 
ATGGCGGCCC TGGGTGTAGC GGTAGACGGT GGCAAGGACT CCCTCAGCAT GGCTGCCCGG 
GTTGGCTCTG AAACCGTGCG GGCTCCTGGG TCACTGGTCA TCTCAGCCTA TGCTGTCTGT 
TCAGACATTA CAGCCACTGT GACCCCAGAC CTCAAGCGTC CTGGGGGGAG AGGCCACCTG 
CTCTACGTGC CTCTGAGTCC TGGGCAGCAC CGGCTGGGGG GCACAGCTCT GGCTCAGTGC 
TTCTCCCAGC TTGGCGAGCA GCCTCCCGAC CTGGATGTTC CTGAGAACTT GGTGCGAGCC 
TTCAGCATCA CCCAGGGGCT GCTGAAAGAC CGCCTCCTCT GCTCAGGCCA TGATGTCAGT 
GATGGAGGTC TCATCACTTG CCTGCTAGAG ATGGCCTTTG CTGGGAATTG TGGGATAGAG 
GTGGACATCC CCGCACCTGG GGTCGATGCC CTGCCTGTGC TGTTCGCCGA GGAGCCAGGC 
CTGGTGCTGG AGGTGCAGGA GCCGGACCTC GCCCAAGTGC TGATGCGTTA CCGGAATGCT 
GGCCTCCACT GCCTAGAGCT GGGTCTCACA GGCGACACGG GGCCCCACGC CATGGTCCGC 
GTGTCGGTGA ACAGGACGGT GGTTCTGGAG GAACCTGTTG GGCAGCTACG AGCCCTCTGG 
GAGGAAACCA GTTTCCAGTT GGACCGGCTA CAGGCAAAAC CACACTGTGT GGCCCAGGAA 
GAGCAAGGGC TGAGGGAGCG GGCAGGGCCC ACCTTCTGCC TGCCGCCTAC CTTCCCCAGA 
GCTTCCGTGC CTTGTGAGCC TGGTGGTCCT GCCCCCCGAG TCGCCATCAT TCGGGAAGAG 
GGCAGTAATG GAGACCGGGA GATGGCAGAT GCCTTCCACT TGGCTGGATT TGAGGTGTGG 
GATGTAACCA TGCAGGACCT GTGCTCTGGA GCCATCGGGC TGGACACATT CCGCGGTGTG 
GCCTTCGTAG GCGGCTTCAG CTACGCGGAC GTCCTGGGCT CTGCCAAAGG CTGGGCAGCT 
GCTGTGACCT TTAACCCGCA GGCCGGGGCT GAGATGAAGC GCTTCCGCCA GCGGCCAGAC 
ACCTTCAGCC TGGGCGTGTG TAACGGCTGT CAGCTGCTGG CCCTGCTGGG CTGGGTGGGA 
GGCAGTTCTA GTGAGGAGGT GGTGGAGATG TGCCAGGAGT CCTGGCCGGC CCGGCCCGGT 
CTCCTGCTAC GCCACAACCT GTCTGGGCGC TTTGAGTCGC GCTGGGCTAG TGTGCGCGTG 
GGGCCCGGGC CAGCCCTGAT GCTGCGAGGG ATGGAAGGCG CGGTGCTGCC CGTGTGGAGC 
GCTCACGGGG AAGGTTACAT GGCATTTTCT TCTCCGGAAC TCCAAGCCCA GATGGAGGCC 
AGGGGCTTAG CTCCACTGCA CTGGGCAGAT GATGATGGGA ACCCCACGGA ACAGTACCCC 
CTGAATCCCA ACGGCTCCCC AGGGGGTGTC GCTGGCATCT GCTCCCCCGA TGGCCGCCAC 
TTGGCCCTCA TGCCTCACCC GGAGCGGTCT GTGAGGCCTT GGCAGTGGGC CTGGCGACCT 
CCTCCATTTG ACACTCTAAC CACCTCCCCC TGGCTCCAGC TCTTCATCAA TGCCCGGAAT 
TGGACCCAGG AATGCAGCTG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005220416.3
CDS	19..4122
Translation	MRMTLICAPEFEELTDWEKCYHAQRTPAEMSSVLHFYVRPSGHERAASEYIQRKLQRELPELQGVKTEQCYNVNWAAESFPSNKEMKKLTWLFGCPLLLDDVAQESWLRPGPTDLLLEVGPRLNFSTPTSSNIVSVCQAAGLGAVDRVEPTRRYLLSFAHPPSADLEAIALATLHDRMTEQHFPQPIQSFSSGCIPAPLSGPIDVLAEGRSALEKANQELGLALDSWDLDFYTKRFQELQRNPSTVEVFDLAQSNSEHSRHWFFKGRLHVDGQELPHSLFESIMSTQASSNPNNVLKFCDNSSAIQGKEVRFLRPKDPTRPSSFQQCQGLRHVVFTAETHNFPTGVAPFSGAATGTGGRIRDVQCTGRGAHVVAGTAGYCFGNLHIPGYSMPWEDPSFQYPGNFARPLEIAIEASNGASDYGNKFGEPVLAGFARSLGLQLPDGQRREWIKPIMFSGGIGSMEAEHVSKEPPETGMDVVKIGGPVYRIGVGGGAASSVQVQGDNASDLDFGAVQRGDPEMEQKMNRVIRACVEAPGGNPICSLHDQGAGGNGNVLKELSDPAGAVIHTSCFQLGDPTLNALEIWGAEYQESNALLLRPSDRDFLSCVSARERCPVCFVGTITGDRRIVLVDDREWPMGTGGQVDAPPTPPPTPVDLDLDWVLGKMPQKEFFLQRSLPLLQPLTLPPGLSVRQALARVLRLPAVASKRYLTNKVDRSVGGLVAQQQCVGPLQTPLADVAVVALSHQELVGAATALGEQPVKSLLDPKVAARLAVAEALTNLVFALVTDLQDVKCSGNWMWAAKLPGEGAALADACEAMVAVMAALGVAVDGGKDSLSMAARVGSETVRAPGSLVISAYAVCSDITATVTPDLKRPGGRGHLLYVPLSPGQHRLGGTALAQCFSQLGEQPPDLDVPENLVRAFSITQGLLKDRLLCSGHDVSDGGLITCLLEMAFAGNCGIEVDIPAPGVDALPVLFAEEPGLVLEVQEPDLAQVLMRYRNAGLHCLELGLTGDTGPHAMVRVSVNRTVVLEEPVGQLRALWEETSFQLDRLQAKPHCVAQEEQGLRERAGPTFCLPPTFPRASVPCEPGGPAPRVAIIREEGSNGDREMADAFHLAGFEVWDVTMQDLCSGAIGLDTFRGVAFVGGFSYADVLGSAKGWAAAVTFNPQAGAEMKRFRQRPDTFSLGVCNGCQLLALLGWVGGSSSEEVVEMCQESWPARPGLLLRHNLSGRFESRWASVRVGPGPALMLRGMEGAVLPVWSAHGEGYMAFSSPELQAQMEARGLAPLHWADDDGNPTEQYPLNPNGSPGGVAGICSPDGRHLALMPHPERSVRPWQWAWRPPPFDTLTTSPWLQLFINARNWTQECSC

Target ORF information:

RefSeq Version	XM_005220359.3
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005220359.3

ORF Insert Sequence:

ATGAGGATGA CGTTGATCTG CGCGCCAGAA TTCGAGGAGC TGACGGACTG GGAGAAGTGC 
TACCACGCGC AACGGACACC TGCAGAGATG TCCTCCGTCC TTCACTTCTA TGTCCGCCCC 
TCTGGCCACG AGAGGGCAGC CTCTGAATAC ATTCAAAGGA AGCTGCAAAG GGAACTGCCA 
GAGCTGCAGG GTGTCAAGAC CGAGCAGTGC TACAATGTGA ACTGGGCAGC GGAGTCCTTC 
CCGAGCAACA AGGAGATGAA GAAGCTGACA TGGCTATTTG GCTGCCCCTT GTTACTGGAC 
GATGTTGCTC AGGAATCCTG GCTTCGTCCT GGCCCCACAG ACCTGCTGCT GGAGGTCGGA 
CCCAGGCTGA ACTTTTCCAC CCCGACATCC AGCAACATTG TGTCAGTGTG CCAGGCTGCA 
GGGCTGGGGG CTGTGGACCG CGTGGAGCCA ACCCGGCGCT ACCTGCTCTC GTTTGCCCAC 
CCACCTTCAG CTGACCTGGA GGCCATTGCC CTGGCTACCC TGCATGACCG GATGACAGAG 
CAGCACTTCC CCCAGCCCAT CCAGAGCTTC TCTTCAGGGT GCATCCCAGC ACCCCTGAGC 
GGCCCAATTG ACGTGCTGGC TGAGGGCCGG TCTGCCCTGG AGAAAGCCAA CCAGGAGCTA 
GGTCTGGCCC TAGACTCCTG GGACCTGGAT TTCTACACCA AGCGCTTCCA GGAGCTGCAG 
CGCAACCCCA GCACTGTGGA AGTCTTCGAC TTGGCTCAGT CCAATAGCGA ACACAGCCGA 
CACTGGTTCT TCAAGGGTCG ACTCCATGTG GATGGACAGG AGCTGCCACA CTCGCTGTTT 
GAGTCCATCA TGAGCACCCA GGCGTCCTCC AACCCTAACA ACGTCCTCAA GTTCTGTGAC 
AACAGCAGTG CCATCCAGGG GAAAGAAGTC CGATTCTTGC GGCCTAAGGA CCCCACACGG 
CCAAGCTCCT TCCAGCAATG TCAGGGGCTG AGACATGTTG TCTTCACAGC AGAGACACAC 
AACTTCCCTA CAGGAGTAGC CCCCTTCAGT GGTGCAGCCA CTGGCACAGG GGGCCGGATC 
CGAGATGTCC AGTGCACAGG CCGAGGGGCC CACGTGGTGG CTGGCACTGC TGGCTATTGC 
TTTGGAAACC TGCACATCCC AGGTTACAGT ATGCCCTGGG AGGATCCAAG CTTCCAGTAT 
CCAGGAAACT TTGCCCGACC CCTGGAGATT GCCATTGAGG CCAGTAATGG GGCTTCTGAC 
TACGGCAACA AGTTTGGGGA ACCAGTGCTT GCTGGCTTTG CCCGCTCCTT GGGCCTTCAG 
CTTCCGGACG GCCAGCGGCG TGAGTGGATC AAGCCCATAA TGTTTAGTGG AGGCATTGGG 
TCCATGGAAG CTGAGCATGT GAGCAAGGAG CCCCCAGAGA CAGGCATGGA TGTCGTGAAG 
ATTGGCGGTC CTGTCTACAG GATTGGAGTT GGGGGCGGAG CTGCTTCGTC TGTGCAGGTG 
CAAGGAGACA ACGCCAGTGA CCTGGATTTT GGGGCTGTGC AGCGGGGAGA CCCAGAGATG 
GAGCAGAAGA TGAACCGTGT GATCCGGGCT TGTGTGGAGG CCCCTGGGGG AAACCCCATC 
TGCAGCCTCC ATGACCAGGG TGCTGGTGGC AATGGCAATG TCCTGAAGGA GCTGAGTGAC 
CCGGCTGGAG CCGTCATTCA CACCAGCTGC TTCCAGCTGG GGGACCCAAC CCTGAATGCC 
CTGGAAATCT GGGGGGCCGA GTACCAGGAG TCGAACGCAC TTCTGCTGAG GCCCTCTGAC 
CGGGACTTCC TGAGTTGTGT CAGCGCCCGG GAACGCTGCC CAGTCTGCTT TGTGGGCACC 
ATCACTGGAG ACAGGAGAAT AGTGCTGGTG GATGATCGGG AGTGGCCTAT GGGCACAGGT 
GGCCAGGTGG ATGCGCCCCC CACTCCTCCC CCAACCCCTG TGGACCTGGA CCTGGACTGG 
GTGCTGGGCA AGATGCCCCA GAAGGAGTTC TTCCTCCAGA GGAGTCTCCC CTTGCTGCAG 
CCTCTGACTC TGCCCCCAGG GCTGAGCGTG CGCCAGGCTC TGGCGCGGGT CCTCAGGCTG 
CCTGCCGTGG CCAGCAAGCG CTACCTCACC AACAAGGTGG ACCGTTCTGT GGGCGGCCTG 
GTGGCCCAGC AGCAGTGTGT GGGACCCCTG CAGACCCCTC TGGCAGATGT GGCGGTTGTG 
GCACTGAGTC ACCAGGAGCT TGTAGGGGCC GCCACAGCCC TGGGAGAGCA GCCAGTCAAG 
AGCCTGCTGG ACCCGAAGGT TGCCGCCCGG CTGGCTGTGG CCGAAGCCCT CACCAACCTG 
GTATTTGCTC TGGTCACCGA CCTCCAGGAT GTGAAATGCA GCGGGAACTG GATGTGGGCA 
GCCAAGCTCC CAGGGGAGGG TGCAGCTCTG GCTGACGCCT GTGAGGCTAT GGTGGCAGTG 
ATGGCGGCCC TGGGTGTAGC GGTAGACGGT GGCAAGGACT CCCTCAGCAT GGCTGCCCGG 
GTTGGCTCTG AAACCGTGCG GGCTCCTGGG TCACTGGTCA TCTCAGCCTA TGCTGTCTGT 
TCAGACATTA CAGCCACTGT GACCCCAGAC CTCAAGCGTC CTGGGGGGAG AGGCCACCTG 
CTCTACGTGC CTCTGAGTCC TGGGCAGCAC CGGCTGGGGG GCACAGCTCT GGCTCAGTGC 
TTCTCCCAGC TTGGCGAGCA GCCTCCCGAC CTGGATGTTC CTGAGAACTT GGTGCGAGCC 
TTCAGCATCA CCCAGGGGCT GCTGAAAGAC CGCCTCCTCT GCTCAGGCCA TGATGTCAGT 
GATGGAGGTC TCATCACTTG CCTGCTAGAG ATGGCCTTTG CTGGGAATTG TGGGATAGAG 
GTGGACATCC CCGCACCTGG GGTCGATGCC CTGCCTGTGC TGTTCGCCGA GGAGCCAGGC 
CTGGTGCTGG AGGTGCAGGA GCCGGACCTC GCCCAAGTGC TGATGCGTTA CCGGAATGCT 
GGCCTCCACT GCCTAGAGCT GGGTCTCACA GGCGACACGG GGCCCCACGC CATGGTCCGC 
GTGTCGGTGA ACAGGACGGT GGTTCTGGAG GAACCTGTTG GGCAGCTACG AGCCCTCTGG 
GAGGAAACCA GTTTCCAGTT GGACCGGCTA CAGGCAAAAC CACACTGTGT GGCCCAGGAA 
GAGCAAGGGC TGAGGGAGCG GGCAGGGCCC ACCTTCTGCC TGCCGCCTAC CTTCCCCAGA 
GCTTCCGTGC CTTGTGAGCC TGGTGGTCCT GCCCCCCGAG TCGCCATCAT TCGGGAAGAG 
GGCAGTAATG GAGACCGGGA GATGGCAGAT GCCTTCCACT TGGCTGGATT TGAGGTGTGG 
GATGTAACCA TGCAGGACCT GTGCTCTGGA GCCATCGGGC TGGACACATT CCGCGGTGTG 
GCCTTCGTAG GCGGCTTCAG CTACGCGGAC GTCCTGGGCT CTGCCAAAGG CTGGGCAGCT 
GCTGTGACCT TTAACCCGCA GGCCGGGGCT GAGATGAAGC GCTTCCGCCA GCGGCCAGAC 
ACCTTCAGCC TGGGCGTGTG TAACGGCTGT CAGCTGCTGG CCCTGCTGGG CTGGGTGGGA 
GGCAGTTCTA GTGAGGAGGT GGTGGAGATG TGCCAGGAGT CCTGGCCGGC CCGGCCCGGT 
CTCCTGCTAC GCCACAACCT GTCTGGGCGC TTTGAGTCGC GCTGGGCTAG TGTGCGCGTG 
GGGCCCGGGC CAGCCCTGAT GCTGCGAGGG ATGGAAGGCG CGGTGCTGCC CGTGTGGAGC 
GCTCACGGGG AAGGTTACAT GGCATTTTCT TCTCCGGAAC TCCAAGCCCA GATGGAGGCC 
AGGGGCTTAG CTCCACTGCA CTGGGCAGAT GATGATGGGA ACCCCACGGA ACAGTACCCC 
CTGAATCCCA ACGGCTCCCC AGGGGGTGTC GCTGGCATCT GCTCCCCCGA TGGCCGCCAC 
TTGGCCCTCA TGCCTCACCC GGAGCGGTCT GTGAGGCCTT GGCAGTGGGC CTGGCGACCT 
CCTCCATTTG ACACTCTAAC CACCTCCCCC TGGCTCCAGC TCTTCATCAA TGCCCGGAAT 
TGGACCCAGG AATGCAGCTG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFAS ( NM_001256564.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001243493.1 Bos taurus phosphoribosylformylglycinamidine synthase (PFAS), mRNA.

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CloneID	OBa29748
Clone ID Related Accession (Same CDS sequence)	XM_010816030.2 , NM_001256564.1
Accession Version	NM_001256564.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-22
Organism	Bos taurus(cattle)
Product	phosphoribosylformylglycinamidine synthase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DAAA02048815.1. On or before Feb 11, 2012 this sequence version replaced XM_598557.4, XM_002695870.1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN02822088, SAMN02822089 [ECO:0000348] ##Evidence-Data-END##

ATGTCCTCCG TCCTTCACTT CTATGTCCGC CCCTCTGGCC ACGAGAGGGC AGCCTCTGAA 
TACATTCAAA GGAAGCTGCA AAGGGAACTG CCAGAGCTGC AGGGTGTCAA GACCGAGCAG 
TGCTACAATG TGAACTGGGC AGCGGAGTCC TTCCCGAGCA ACAAGGAGAT GAAGAAGCTG 
ACATGGCTAT TTGGCTGCCC CTTGTTACTG GACGATGTTG CTCAGGAATC CTGGCTTCGT 
CCTGGCCCCA CAGACCTGCT GCTGGAGGTC GGACCCAGGC TGAACTTTTC CACCCCGACA 
TCCAGCAACA TTGTGTCAGT GTGCCAGGCT GCAGGGCTGG GGGCTGTGGA CCGCGTGGAG 
CCAACCCGGC GCTACCTGCT CTCGTTTGCC CACCCACCTT CAGCTGACCT GGAGGCCATT 
GCCCTGGCTA CCCTGCATGA CCGGATGACA GAGCAGCACT TCCCCCAGCC CATCCAGAGC 
TTCTCTTCAG GGTGCATCCC AGCACCCCTG AGCGGCCCAA TTGACGTGCT GGCTGAGGGC 
CGGTCTGCCC TGGAGAAAGC CAACCAGGAG CTAGGTCTGG CCCTAGACTC CTGGGACCTG 
GATTTCTACA CCAAGCGCTT CCAGGAGCTG CAGCGCAACC CCAGCACTGT GGAAGTCTTC 
GACTTGGCTC AGTCCAATAG CGAACACAGC CGACACTGGT TCTTCAAGGG TCGACTCCAT 
GTGGATGGAC AGGAGCTGCC ACACTCGCTG TTTGAGTCCA TCATGAGCAC CCAGGCGTCC 
TCCAACCCTA ACAACGTCCT CAAGTTCTGT GACAACAGCA GTGCCATCCA GGGGAAAGAA 
GTCCGATTCT TGCGGCCTAA GGACCCCACA CGGCCAAGCT CCTTCCAGCA ATGTCAGGGG 
CTGAGACATG TTGTCTTCAC AGCAGAGACA CACAACTTCC CTACAGGAGT AGCCCCCTTC 
AGTGGTGCAG CCACTGGCAC AGGGGGCCGG ATCCGAGATG TCCAGTGCAC AGGCCGAGGG 
GCCCACGTGG TGGCTGGCAC TGCTGGCTAT TGCTTTGGAA ACCTGCACAT CCCAGGTTAC 
AGTATGCCCT GGGAGGATCC AAGCTTCCAG TATCCAGGAA ACTTTGCCCG ACCCCTGGAG 
ATTGCCATTG AGGCCAGTAA TGGGGCTTCT GACTACGGCA ACAAGTTTGG GGAACCAGTG 
CTTGCTGGCT TTGCCCGCTC CTTGGGCCTT CAGCTTCCGG ACGGCCAGCG GCGTGAGTGG 
ATCAAGCCCA TAATGTTTAG TGGAGGCATT GGGTCCATGG AAGCTGAGCA TGTGAGCAAG 
GAGCCCCCAG AGACAGGCAT GGATGTCGTG AAGATTGGCG GTCCTGTCTA CAGGATTGGA 
GTTGGGGGCG GAGCTGCTTC GTCTGTGCAG GTGCAAGGAG ACAACGCCAG TGACCTGGAT 
TTTGGGGCTG TGCAGCGGGG AGACCCAGAG ATGGAGCAGA AGATGAACCG TGTGATCCGG 
GCTTGTGTGG AGGCCCCTGG GGGAAACCCC ATCTGCAGCC TCCATGACCA GGGTGCTGGT 
GGCAATGGCA ATGTCCTGAA GGAGCTGAGT GACCCGGCTG GAGCCGTCAT TCACACCAGC 
TGCTTCCAGC TGGGGGACCC AACCCTGAAT GCCCTGGAAA TCTGGGGGGC CGAGTACCAG 
GAGTCGAACG CACTTCTGCT GAGGCCCTCT GACCGGGACT TCCTGAGTTG TGTCAGCGCC 
CGGGAACGCT GCCCAGTCTG CTTTGTGGGC ACCATCACTG GAGACAGGAG AATAGTGCTG 
GTGGATGATC GGGAGTGGCC TATGGGCACA GGTGGCCAGG TGGATGCGCC CCCCACTCCT 
CCCCCAACCC CTGTGGACCT GGACCTGGAC TGGGTGCTGG GCAAGATGCC CCAGAAGGAG 
TTCTTCCTCC AGAGGAGTCT CCCCTTGCTG CAGCCTCTGA CTCTGCCCCC AGGGCTGAGC 
GTGCGCCAGG CTCTGGCGCG GGTCCTCAGG CTGCCTGCCG TGGCCAGCAA GCGCTACCTC 
ACCAACAAGG TGGACCGTTC TGTGGGCGGC CTGGTGGCCC AGCAGCAGTG TGTGGGACCC 
CTGCAGACCC CTCTGGCAGA TGTGGCGGTT GTGGCACTGA GTCACCAGGA GCTTGTAGGG 
GCCGCCACAG CCCTGGGAGA GCAGCCAGTC AAGAGCCTGC TGGACCCGAA GGTTGCCGCC 
CGGCTGGCTG TGGCCGAAGC CCTCACCAAC CTGGTATTTG CTCTGGTCAC CGACCTCCAG 
GATGTGAAAT GCAGCGGGAA CTGGATGTGG GCAGCCAAGC TCCCAGGGGA GGGTGCAGCT 
CTGGCTGACG CCTGTGAGGC TATGGTGGCA GTGATGGCGG CCCTGGGTGT AGCGGTAGAC 
GGTGGCAAGG ACTCCCTCAG CATGGCTGCC CGGGTTGGCT CTGAAACCGT GCGGGCTCCT 
GGGTCACTGG TCATCTCAGC CTATGCTGTC TGTTCAGACA TTACAGCCAC TGTGACCCCA 
GACCTCAAGC GTCCTGGGGG GAGAGGCCAC CTGCTCTACG TGCCTCTGAG TCCTGGGCAG 
CACCGGCTGG GGGGCACAGC TCTGGCTCAG TGCTTCTCCC AGCTTGGCGA GCAGCCTCCC 
GACCTGGATG TTCCTGAGAA CTTGGTGCGA GCCTTCAGCA TCACCCAGGG GCTGCTGAAA 
GACCGCCTCC TCTGCTCAGG CCATGATGTC AGTGATGGAG GTCTCATCAC TTGCCTGCTA 
GAGATGGCCT TTGCTGGGAA TTGTGGGATA GAGGTGGACA TCCCCGCACC TGGGGTCGAT 
GCCCTGCCTG TGCTGTTCGC CGAGGAGCCA GGCCTGGTGC TGGAGGTGCA GGAGCCGGAC 
CTCGCCCAAG TGCTGATGCG TTACCGGAAT GCTGGCCTCC ACTGCCTAGA GCTGGGTCTC 
ACAGGCGACA CGGGGCCCCA CGCCATGGTC CGCGTGTCGG TGAACAGGAC GGTGGTTCTG 
GAGGAACCTG TTGGGCAGCT ACGAGCCCTC TGGGAGGAAA CCAGTTTCCA GTTGGACCGG 
CTACAGGCAA AACCACACTG TGTGGCCCAG GAAGAGCAAG GGCTGAGGGA GCGGGCAGGG 
CCCACCTTCT GCCTGCCGCC TACCTTCCCC AGAGCTTCCG TGCCTTGTGA GCCTGGTGGT 
CCTGCCCCCC GAGTCGCCAT CATTCGGGAA GAGGGCAGTA ATGGAGACCG GGAGATGGCA 
GATGCCTTCC ACTTGGCTGG ATTTGAGGTG TGGGATGTAA CCATGCAGGA CCTGTGCTCT 
GGAGCCATCG GGCTGGACAC ATTCCGCGGT GTGGCCTTCG TAGGCGGCTT CAGCTACGCG 
GACGTCCTGG GCTCTGCCAA AGGCTGGGCA GCTGCTGTGA CCTTTAACCC GCAGGCCGGG 
GCTGAGATGA AGCGCTTCCG CCAGCGGCCA GACACCTTCA GCCTGGGCGT GTGTAACGGC 
TGTCAGCTGC TGGCCCTGCT GGGCTGGGTG GGAGGCAGTT CTAGTGAGGA GGTGGTGGAG 
ATGTGCCAGG AGTCCTGGCC GGCCCGGCCC GGTCTCCTGC TACGCCACAA CCTGTCTGGG 
CGCTTTGAGT CGCGCTGGGC TAGTGTGCGC GTGGGGCCCG GGCCAGCCCT GATGCTGCGA 
GGGATGGAAG GCGCGGTGCT GCCCGTGTGG AGCGCTCACG GGGAAGGTTA CATGGCATTT 
TCTTCTCCGG AACTCCAAGC CCAGATGGAG GCCAGGGGCT TAGCTCCACT GCACTGGGCA 
GATGATGATG GGAACCCCAC GGAACAGTAC CCCCTGAATC CCAACGGCTC CCCAGGGGGT 
GTCGCTGGCA TCTGCTCCCC CGATGGCCGC CACTTGGCCC TCATGCCTCA CCCGGAGCGG 
TCTGTGAGGC CTTGGCAGTG GGCCTGGCGA CCTCCTCCAT TTGACACTCT AACCACCTCC 
CCCTGGCTCC AGCTCTTCAT CAATGCCCGG AATTGGACCC AGGAATGCAG CTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001243493.1
CDS	1..4017
Translation	MSSVLHFYVRPSGHERAASEYIQRKLQRELPELQGVKTEQCYNVNWAAESFPSNKEMKKLTWLFGCPLLLDDVAQESWLRPGPTDLLLEVGPRLNFSTPTSSNIVSVCQAAGLGAVDRVEPTRRYLLSFAHPPSADLEAIALATLHDRMTEQHFPQPIQSFSSGCIPAPLSGPIDVLAEGRSALEKANQELGLALDSWDLDFYTKRFQELQRNPSTVEVFDLAQSNSEHSRHWFFKGRLHVDGQELPHSLFESIMSTQASSNPNNVLKFCDNSSAIQGKEVRFLRPKDPTRPSSFQQCQGLRHVVFTAETHNFPTGVAPFSGAATGTGGRIRDVQCTGRGAHVVAGTAGYCFGNLHIPGYSMPWEDPSFQYPGNFARPLEIAIEASNGASDYGNKFGEPVLAGFARSLGLQLPDGQRREWIKPIMFSGGIGSMEAEHVSKEPPETGMDVVKIGGPVYRIGVGGGAASSVQVQGDNASDLDFGAVQRGDPEMEQKMNRVIRACVEAPGGNPICSLHDQGAGGNGNVLKELSDPAGAVIHTSCFQLGDPTLNALEIWGAEYQESNALLLRPSDRDFLSCVSARERCPVCFVGTITGDRRIVLVDDREWPMGTGGQVDAPPTPPPTPVDLDLDWVLGKMPQKEFFLQRSLPLLQPLTLPPGLSVRQALARVLRLPAVASKRYLTNKVDRSVGGLVAQQQCVGPLQTPLADVAVVALSHQELVGAATALGEQPVKSLLDPKVAARLAVAEALTNLVFALVTDLQDVKCSGNWMWAAKLPGEGAALADACEAMVAVMAALGVAVDGGKDSLSMAARVGSETVRAPGSLVISAYAVCSDITATVTPDLKRPGGRGHLLYVPLSPGQHRLGGTALAQCFSQLGEQPPDLDVPENLVRAFSITQGLLKDRLLCSGHDVSDGGLITCLLEMAFAGNCGIEVDIPAPGVDALPVLFAEEPGLVLEVQEPDLAQVLMRYRNAGLHCLELGLTGDTGPHAMVRVSVNRTVVLEEPVGQLRALWEETSFQLDRLQAKPHCVAQEEQGLRERAGPTFCLPPTFPRASVPCEPGGPAPRVAIIREEGSNGDREMADAFHLAGFEVWDVTMQDLCSGAIGLDTFRGVAFVGGFSYADVLGSAKGWAAAVTFNPQAGAEMKRFRQRPDTFSLGVCNGCQLLALLGWVGGSSSEEVVEMCQESWPARPGLLLRHNLSGRFESRWASVRVGPGPALMLRGMEGAVLPVWSAHGEGYMAFSSPELQAQMEARGLAPLHWADDDGNPTEQYPLNPNGSPGGVAGICSPDGRHLALMPHPERSVRPWQWAWRPPPFDTLTTSPWLQLFINARNWTQECSC

Target ORF information:

RefSeq Version	NM_001256564.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphoribosylformylglycinamidine synthase (PFAS), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001256564.1

ORF Insert Sequence:

ATGTCCTCCG TCCTTCACTT CTATGTCCGC CCCTCTGGCC ACGAGAGGGC AGCCTCTGAA 
TACATTCAAA GGAAGCTGCA AAGGGAACTG CCAGAGCTGC AGGGTGTCAA GACCGAGCAG 
TGCTACAATG TGAACTGGGC AGCGGAGTCC TTCCCGAGCA ACAAGGAGAT GAAGAAGCTG 
ACATGGCTAT TTGGCTGCCC CTTGTTACTG GACGATGTTG CTCAGGAATC CTGGCTTCGT 
CCTGGCCCCA CAGACCTGCT GCTGGAGGTC GGACCCAGGC TGAACTTTTC CACCCCGACA 
TCCAGCAACA TTGTGTCAGT GTGCCAGGCT GCAGGGCTGG GGGCTGTGGA CCGCGTGGAG 
CCAACCCGGC GCTACCTGCT CTCGTTTGCC CACCCACCTT CAGCTGACCT GGAGGCCATT 
GCCCTGGCTA CCCTGCATGA CCGGATGACA GAGCAGCACT TCCCCCAGCC CATCCAGAGC 
TTCTCTTCAG GGTGCATCCC AGCACCCCTG AGCGGCCCAA TTGACGTGCT GGCTGAGGGC 
CGGTCTGCCC TGGAGAAAGC CAACCAGGAG CTAGGTCTGG CCCTAGACTC CTGGGACCTG 
GATTTCTACA CCAAGCGCTT CCAGGAGCTG CAGCGCAACC CCAGCACTGT GGAAGTCTTC 
GACTTGGCTC AGTCCAATAG CGAACACAGC CGACACTGGT TCTTCAAGGG TCGACTCCAT 
GTGGATGGAC AGGAGCTGCC ACACTCGCTG TTTGAGTCCA TCATGAGCAC CCAGGCGTCC 
TCCAACCCTA ACAACGTCCT CAAGTTCTGT GACAACAGCA GTGCCATCCA GGGGAAAGAA 
GTCCGATTCT TGCGGCCTAA GGACCCCACA CGGCCAAGCT CCTTCCAGCA ATGTCAGGGG 
CTGAGACATG TTGTCTTCAC AGCAGAGACA CACAACTTCC CTACAGGAGT AGCCCCCTTC 
AGTGGTGCAG CCACTGGCAC AGGGGGCCGG ATCCGAGATG TCCAGTGCAC AGGCCGAGGG 
GCCCACGTGG TGGCTGGCAC TGCTGGCTAT TGCTTTGGAA ACCTGCACAT CCCAGGTTAC 
AGTATGCCCT GGGAGGATCC AAGCTTCCAG TATCCAGGAA ACTTTGCCCG ACCCCTGGAG 
ATTGCCATTG AGGCCAGTAA TGGGGCTTCT GACTACGGCA ACAAGTTTGG GGAACCAGTG 
CTTGCTGGCT TTGCCCGCTC CTTGGGCCTT CAGCTTCCGG ACGGCCAGCG GCGTGAGTGG 
ATCAAGCCCA TAATGTTTAG TGGAGGCATT GGGTCCATGG AAGCTGAGCA TGTGAGCAAG 
GAGCCCCCAG AGACAGGCAT GGATGTCGTG AAGATTGGCG GTCCTGTCTA CAGGATTGGA 
GTTGGGGGCG GAGCTGCTTC GTCTGTGCAG GTGCAAGGAG ACAACGCCAG TGACCTGGAT 
TTTGGGGCTG TGCAGCGGGG AGACCCAGAG ATGGAGCAGA AGATGAACCG TGTGATCCGG 
GCTTGTGTGG AGGCCCCTGG GGGAAACCCC ATCTGCAGCC TCCATGACCA GGGTGCTGGT 
GGCAATGGCA ATGTCCTGAA GGAGCTGAGT GACCCGGCTG GAGCCGTCAT TCACACCAGC 
TGCTTCCAGC TGGGGGACCC AACCCTGAAT GCCCTGGAAA TCTGGGGGGC CGAGTACCAG 
GAGTCGAACG CACTTCTGCT GAGGCCCTCT GACCGGGACT TCCTGAGTTG TGTCAGCGCC 
CGGGAACGCT GCCCAGTCTG CTTTGTGGGC ACCATCACTG GAGACAGGAG AATAGTGCTG 
GTGGATGATC GGGAGTGGCC TATGGGCACA GGTGGCCAGG TGGATGCGCC CCCCACTCCT 
CCCCCAACCC CTGTGGACCT GGACCTGGAC TGGGTGCTGG GCAAGATGCC CCAGAAGGAG 
TTCTTCCTCC AGAGGAGTCT CCCCTTGCTG CAGCCTCTGA CTCTGCCCCC AGGGCTGAGC 
GTGCGCCAGG CTCTGGCGCG GGTCCTCAGG CTGCCTGCCG TGGCCAGCAA GCGCTACCTC 
ACCAACAAGG TGGACCGTTC TGTGGGCGGC CTGGTGGCCC AGCAGCAGTG TGTGGGACCC 
CTGCAGACCC CTCTGGCAGA TGTGGCGGTT GTGGCACTGA GTCACCAGGA GCTTGTAGGG 
GCCGCCACAG CCCTGGGAGA GCAGCCAGTC AAGAGCCTGC TGGACCCGAA GGTTGCCGCC 
CGGCTGGCTG TGGCCGAAGC CCTCACCAAC CTGGTATTTG CTCTGGTCAC CGACCTCCAG 
GATGTGAAAT GCAGCGGGAA CTGGATGTGG GCAGCCAAGC TCCCAGGGGA GGGTGCAGCT 
CTGGCTGACG CCTGTGAGGC TATGGTGGCA GTGATGGCGG CCCTGGGTGT AGCGGTAGAC 
GGTGGCAAGG ACTCCCTCAG CATGGCTGCC CGGGTTGGCT CTGAAACCGT GCGGGCTCCT 
GGGTCACTGG TCATCTCAGC CTATGCTGTC TGTTCAGACA TTACAGCCAC TGTGACCCCA 
GACCTCAAGC GTCCTGGGGG GAGAGGCCAC CTGCTCTACG TGCCTCTGAG TCCTGGGCAG 
CACCGGCTGG GGGGCACAGC TCTGGCTCAG TGCTTCTCCC AGCTTGGCGA GCAGCCTCCC 
GACCTGGATG TTCCTGAGAA CTTGGTGCGA GCCTTCAGCA TCACCCAGGG GCTGCTGAAA 
GACCGCCTCC TCTGCTCAGG CCATGATGTC AGTGATGGAG GTCTCATCAC TTGCCTGCTA 
GAGATGGCCT TTGCTGGGAA TTGTGGGATA GAGGTGGACA TCCCCGCACC TGGGGTCGAT 
GCCCTGCCTG TGCTGTTCGC CGAGGAGCCA GGCCTGGTGC TGGAGGTGCA GGAGCCGGAC 
CTCGCCCAAG TGCTGATGCG TTACCGGAAT GCTGGCCTCC ACTGCCTAGA GCTGGGTCTC 
ACAGGCGACA CGGGGCCCCA CGCCATGGTC CGCGTGTCGG TGAACAGGAC GGTGGTTCTG 
GAGGAACCTG TTGGGCAGCT ACGAGCCCTC TGGGAGGAAA CCAGTTTCCA GTTGGACCGG 
CTACAGGCAA AACCACACTG TGTGGCCCAG GAAGAGCAAG GGCTGAGGGA GCGGGCAGGG 
CCCACCTTCT GCCTGCCGCC TACCTTCCCC AGAGCTTCCG TGCCTTGTGA GCCTGGTGGT 
CCTGCCCCCC GAGTCGCCAT CATTCGGGAA GAGGGCAGTA ATGGAGACCG GGAGATGGCA 
GATGCCTTCC ACTTGGCTGG ATTTGAGGTG TGGGATGTAA CCATGCAGGA CCTGTGCTCT 
GGAGCCATCG GGCTGGACAC ATTCCGCGGT GTGGCCTTCG TAGGCGGCTT CAGCTACGCG 
GACGTCCTGG GCTCTGCCAA AGGCTGGGCA GCTGCTGTGA CCTTTAACCC GCAGGCCGGG 
GCTGAGATGA AGCGCTTCCG CCAGCGGCCA GACACCTTCA GCCTGGGCGT GTGTAACGGC 
TGTCAGCTGC TGGCCCTGCT GGGCTGGGTG GGAGGCAGTT CTAGTGAGGA GGTGGTGGAG 
ATGTGCCAGG AGTCCTGGCC GGCCCGGCCC GGTCTCCTGC TACGCCACAA CCTGTCTGGG 
CGCTTTGAGT CGCGCTGGGC TAGTGTGCGC GTGGGGCCCG GGCCAGCCCT GATGCTGCGA 
GGGATGGAAG GCGCGGTGCT GCCCGTGTGG AGCGCTCACG GGGAAGGTTA CATGGCATTT 
TCTTCTCCGG AACTCCAAGC CCAGATGGAG GCCAGGGGCT TAGCTCCACT GCACTGGGCA 
GATGATGATG GGAACCCCAC GGAACAGTAC CCCCTGAATC CCAACGGCTC CCCAGGGGGT 
GTCGCTGGCA TCTGCTCCCC CGATGGCCGC CACTTGGCCC TCATGCCTCA CCCGGAGCGG 
TCTGTGAGGC CTTGGCAGTG GGCCTGGCGA CCTCCTCCAT TTGACACTCT AACCACCTCC 
CCCTGGCTCC AGCTCTTCAT CAATGCCCGG AATTGGACCC AGGAATGCAG CTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFAS ( XM_010816030.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010814332.1 Bos taurus phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X3, mRNA.

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CloneID	OBa29748
Clone ID Related Accession (Same CDS sequence)	XM_010816030.2 , NM_001256564.1
Accession Version	XM_010816030.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	phosphoribosylformylglycinamidine synthase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104494.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010816030.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTCCG TCCTTCACTT CTATGTCCGC CCCTCTGGCC ACGAGAGGGC AGCCTCTGAA 
TACATTCAAA GGAAGCTGCA AAGGGAACTG CCAGAGCTGC AGGGTGTCAA GACCGAGCAG 
TGCTACAATG TGAACTGGGC AGCGGAGTCC TTCCCGAGCA ACAAGGAGAT GAAGAAGCTG 
ACATGGCTAT TTGGCTGCCC CTTGTTACTG GACGATGTTG CTCAGGAATC CTGGCTTCGT 
CCTGGCCCCA CAGACCTGCT GCTGGAGGTC GGACCCAGGC TGAACTTTTC CACCCCGACA 
TCCAGCAACA TTGTGTCAGT GTGCCAGGCT GCAGGGCTGG GGGCTGTGGA CCGCGTGGAG 
CCAACCCGGC GCTACCTGCT CTCGTTTGCC CACCCACCTT CAGCTGACCT GGAGGCCATT 
GCCCTGGCTA CCCTGCATGA CCGGATGACA GAGCAGCACT TCCCCCAGCC CATCCAGAGC 
TTCTCTTCAG GGTGCATCCC AGCACCCCTG AGCGGCCCAA TTGACGTGCT GGCTGAGGGC 
CGGTCTGCCC TGGAGAAAGC CAACCAGGAG CTAGGTCTGG CCCTAGACTC CTGGGACCTG 
GATTTCTACA CCAAGCGCTT CCAGGAGCTG CAGCGCAACC CCAGCACTGT GGAAGTCTTC 
GACTTGGCTC AGTCCAATAG CGAACACAGC CGACACTGGT TCTTCAAGGG TCGACTCCAT 
GTGGATGGAC AGGAGCTGCC ACACTCGCTG TTTGAGTCCA TCATGAGCAC CCAGGCGTCC 
TCCAACCCTA ACAACGTCCT CAAGTTCTGT GACAACAGCA GTGCCATCCA GGGGAAAGAA 
GTCCGATTCT TGCGGCCTAA GGACCCCACA CGGCCAAGCT CCTTCCAGCA ATGTCAGGGG 
CTGAGACATG TTGTCTTCAC AGCAGAGACA CACAACTTCC CTACAGGAGT AGCCCCCTTC 
AGTGGTGCAG CCACTGGCAC AGGGGGCCGG ATCCGAGATG TCCAGTGCAC AGGCCGAGGG 
GCCCACGTGG TGGCTGGCAC TGCTGGCTAT TGCTTTGGAA ACCTGCACAT CCCAGGTTAC 
AGTATGCCCT GGGAGGATCC AAGCTTCCAG TATCCAGGAA ACTTTGCCCG ACCCCTGGAG 
ATTGCCATTG AGGCCAGTAA TGGGGCTTCT GACTACGGCA ACAAGTTTGG GGAACCAGTG 
CTTGCTGGCT TTGCCCGCTC CTTGGGCCTT CAGCTTCCGG ACGGCCAGCG GCGTGAGTGG 
ATCAAGCCCA TAATGTTTAG TGGAGGCATT GGGTCCATGG AAGCTGAGCA TGTGAGCAAG 
GAGCCCCCAG AGACAGGCAT GGATGTCGTG AAGATTGGCG GTCCTGTCTA CAGGATTGGA 
GTTGGGGGCG GAGCTGCTTC GTCTGTGCAG GTGCAAGGAG ACAACGCCAG TGACCTGGAT 
TTTGGGGCTG TGCAGCGGGG AGACCCAGAG ATGGAGCAGA AGATGAACCG TGTGATCCGG 
GCTTGTGTGG AGGCCCCTGG GGGAAACCCC ATCTGCAGCC TCCATGACCA GGGTGCTGGT 
GGCAATGGCA ATGTCCTGAA GGAGCTGAGT GACCCGGCTG GAGCCGTCAT TCACACCAGC 
TGCTTCCAGC TGGGGGACCC AACCCTGAAT GCCCTGGAAA TCTGGGGGGC CGAGTACCAG 
GAGTCGAACG CACTTCTGCT GAGGCCCTCT GACCGGGACT TCCTGAGTTG TGTCAGCGCC 
CGGGAACGCT GCCCAGTCTG CTTTGTGGGC ACCATCACTG GAGACAGGAG AATAGTGCTG 
GTGGATGATC GGGAGTGGCC TATGGGCACA GGTGGCCAGG TGGATGCGCC CCCCACTCCT 
CCCCCAACCC CTGTGGACCT GGACCTGGAC TGGGTGCTGG GCAAGATGCC CCAGAAGGAG 
TTCTTCCTCC AGAGGAGTCT CCCCTTGCTG CAGCCTCTGA CTCTGCCCCC AGGGCTGAGC 
GTGCGCCAGG CTCTGGCGCG GGTCCTCAGG CTGCCTGCCG TGGCCAGCAA GCGCTACCTC 
ACCAACAAGG TGGACCGTTC TGTGGGCGGC CTGGTGGCCC AGCAGCAGTG TGTGGGACCC 
CTGCAGACCC CTCTGGCAGA TGTGGCGGTT GTGGCACTGA GTCACCAGGA GCTTGTAGGG 
GCCGCCACAG CCCTGGGAGA GCAGCCAGTC AAGAGCCTGC TGGACCCGAA GGTTGCCGCC 
CGGCTGGCTG TGGCCGAAGC CCTCACCAAC CTGGTATTTG CTCTGGTCAC CGACCTCCAG 
GATGTGAAAT GCAGCGGGAA CTGGATGTGG GCAGCCAAGC TCCCAGGGGA GGGTGCAGCT 
CTGGCTGACG CCTGTGAGGC TATGGTGGCA GTGATGGCGG CCCTGGGTGT AGCGGTAGAC 
GGTGGCAAGG ACTCCCTCAG CATGGCTGCC CGGGTTGGCT CTGAAACCGT GCGGGCTCCT 
GGGTCACTGG TCATCTCAGC CTATGCTGTC TGTTCAGACA TTACAGCCAC TGTGACCCCA 
GACCTCAAGC GTCCTGGGGG GAGAGGCCAC CTGCTCTACG TGCCTCTGAG TCCTGGGCAG 
CACCGGCTGG GGGGCACAGC TCTGGCTCAG TGCTTCTCCC AGCTTGGCGA GCAGCCTCCC 
GACCTGGATG TTCCTGAGAA CTTGGTGCGA GCCTTCAGCA TCACCCAGGG GCTGCTGAAA 
GACCGCCTCC TCTGCTCAGG CCATGATGTC AGTGATGGAG GTCTCATCAC TTGCCTGCTA 
GAGATGGCCT TTGCTGGGAA TTGTGGGATA GAGGTGGACA TCCCCGCACC TGGGGTCGAT 
GCCCTGCCTG TGCTGTTCGC CGAGGAGCCA GGCCTGGTGC TGGAGGTGCA GGAGCCGGAC 
CTCGCCCAAG TGCTGATGCG TTACCGGAAT GCTGGCCTCC ACTGCCTAGA GCTGGGTCTC 
ACAGGCGACA CGGGGCCCCA CGCCATGGTC CGCGTGTCGG TGAACAGGAC GGTGGTTCTG 
GAGGAACCTG TTGGGCAGCT ACGAGCCCTC TGGGAGGAAA CCAGTTTCCA GTTGGACCGG 
CTACAGGCAA AACCACACTG TGTGGCCCAG GAAGAGCAAG GGCTGAGGGA GCGGGCAGGG 
CCCACCTTCT GCCTGCCGCC TACCTTCCCC AGAGCTTCCG TGCCTTGTGA GCCTGGTGGT 
CCTGCCCCCC GAGTCGCCAT CATTCGGGAA GAGGGCAGTA ATGGAGACCG GGAGATGGCA 
GATGCCTTCC ACTTGGCTGG ATTTGAGGTG TGGGATGTAA CCATGCAGGA CCTGTGCTCT 
GGAGCCATCG GGCTGGACAC ATTCCGCGGT GTGGCCTTCG TAGGCGGCTT CAGCTACGCG 
GACGTCCTGG GCTCTGCCAA AGGCTGGGCA GCTGCTGTGA CCTTTAACCC GCAGGCCGGG 
GCTGAGATGA AGCGCTTCCG CCAGCGGCCA GACACCTTCA GCCTGGGCGT GTGTAACGGC 
TGTCAGCTGC TGGCCCTGCT GGGCTGGGTG GGAGGCAGTT CTAGTGAGGA GGTGGTGGAG 
ATGTGCCAGG AGTCCTGGCC GGCCCGGCCC GGTCTCCTGC TACGCCACAA CCTGTCTGGG 
CGCTTTGAGT CGCGCTGGGC TAGTGTGCGC GTGGGGCCCG GGCCAGCCCT GATGCTGCGA 
GGGATGGAAG GCGCGGTGCT GCCCGTGTGG AGCGCTCACG GGGAAGGTTA CATGGCATTT 
TCTTCTCCGG AACTCCAAGC CCAGATGGAG GCCAGGGGCT TAGCTCCACT GCACTGGGCA 
GATGATGATG GGAACCCCAC GGAACAGTAC CCCCTGAATC CCAACGGCTC CCCAGGGGGT 
GTCGCTGGCA TCTGCTCCCC CGATGGCCGC CACTTGGCCC TCATGCCTCA CCCGGAGCGG 
TCTGTGAGGC CTTGGCAGTG GGCCTGGCGA CCTCCTCCAT TTGACACTCT AACCACCTCC 
CCCTGGCTCC AGCTCTTCAT CAATGCCCGG AATTGGACCC AGGAATGCAG CTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010814332.1
CDS	1030..5046
Translation	MSSVLHFYVRPSGHERAASEYIQRKLQRELPELQGVKTEQCYNVNWAAESFPSNKEMKKLTWLFGCPLLLDDVAQESWLRPGPTDLLLEVGPRLNFSTPTSSNIVSVCQAAGLGAVDRVEPTRRYLLSFAHPPSADLEAIALATLHDRMTEQHFPQPIQSFSSGCIPAPLSGPIDVLAEGRSALEKANQELGLALDSWDLDFYTKRFQELQRNPSTVEVFDLAQSNSEHSRHWFFKGRLHVDGQELPHSLFESIMSTQASSNPNNVLKFCDNSSAIQGKEVRFLRPKDPTRPSSFQQCQGLRHVVFTAETHNFPTGVAPFSGAATGTGGRIRDVQCTGRGAHVVAGTAGYCFGNLHIPGYSMPWEDPSFQYPGNFARPLEIAIEASNGASDYGNKFGEPVLAGFARSLGLQLPDGQRREWIKPIMFSGGIGSMEAEHVSKEPPETGMDVVKIGGPVYRIGVGGGAASSVQVQGDNASDLDFGAVQRGDPEMEQKMNRVIRACVEAPGGNPICSLHDQGAGGNGNVLKELSDPAGAVIHTSCFQLGDPTLNALEIWGAEYQESNALLLRPSDRDFLSCVSARERCPVCFVGTITGDRRIVLVDDREWPMGTGGQVDAPPTPPPTPVDLDLDWVLGKMPQKEFFLQRSLPLLQPLTLPPGLSVRQALARVLRLPAVASKRYLTNKVDRSVGGLVAQQQCVGPLQTPLADVAVVALSHQELVGAATALGEQPVKSLLDPKVAARLAVAEALTNLVFALVTDLQDVKCSGNWMWAAKLPGEGAALADACEAMVAVMAALGVAVDGGKDSLSMAARVGSETVRAPGSLVISAYAVCSDITATVTPDLKRPGGRGHLLYVPLSPGQHRLGGTALAQCFSQLGEQPPDLDVPENLVRAFSITQGLLKDRLLCSGHDVSDGGLITCLLEMAFAGNCGIEVDIPAPGVDALPVLFAEEPGLVLEVQEPDLAQVLMRYRNAGLHCLELGLTGDTGPHAMVRVSVNRTVVLEEPVGQLRALWEETSFQLDRLQAKPHCVAQEEQGLRERAGPTFCLPPTFPRASVPCEPGGPAPRVAIIREEGSNGDREMADAFHLAGFEVWDVTMQDLCSGAIGLDTFRGVAFVGGFSYADVLGSAKGWAAAVTFNPQAGAEMKRFRQRPDTFSLGVCNGCQLLALLGWVGGSSSEEVVEMCQESWPARPGLLLRHNLSGRFESRWASVRVGPGPALMLRGMEGAVLPVWSAHGEGYMAFSSPELQAQMEARGLAPLHWADDDGNPTEQYPLNPNGSPGGVAGICSPDGRHLALMPHPERSVRPWQWAWRPPPFDTLTTSPWLQLFINARNWTQECSC

Target ORF information:

RefSeq Version	XM_010816030.2
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010816030.2

ORF Insert Sequence:

ATGTCCTCCG TCCTTCACTT CTATGTCCGC CCCTCTGGCC ACGAGAGGGC AGCCTCTGAA 
TACATTCAAA GGAAGCTGCA AAGGGAACTG CCAGAGCTGC AGGGTGTCAA GACCGAGCAG 
TGCTACAATG TGAACTGGGC AGCGGAGTCC TTCCCGAGCA ACAAGGAGAT GAAGAAGCTG 
ACATGGCTAT TTGGCTGCCC CTTGTTACTG GACGATGTTG CTCAGGAATC CTGGCTTCGT 
CCTGGCCCCA CAGACCTGCT GCTGGAGGTC GGACCCAGGC TGAACTTTTC CACCCCGACA 
TCCAGCAACA TTGTGTCAGT GTGCCAGGCT GCAGGGCTGG GGGCTGTGGA CCGCGTGGAG 
CCAACCCGGC GCTACCTGCT CTCGTTTGCC CACCCACCTT CAGCTGACCT GGAGGCCATT 
GCCCTGGCTA CCCTGCATGA CCGGATGACA GAGCAGCACT TCCCCCAGCC CATCCAGAGC 
TTCTCTTCAG GGTGCATCCC AGCACCCCTG AGCGGCCCAA TTGACGTGCT GGCTGAGGGC 
CGGTCTGCCC TGGAGAAAGC CAACCAGGAG CTAGGTCTGG CCCTAGACTC CTGGGACCTG 
GATTTCTACA CCAAGCGCTT CCAGGAGCTG CAGCGCAACC CCAGCACTGT GGAAGTCTTC 
GACTTGGCTC AGTCCAATAG CGAACACAGC CGACACTGGT TCTTCAAGGG TCGACTCCAT 
GTGGATGGAC AGGAGCTGCC ACACTCGCTG TTTGAGTCCA TCATGAGCAC CCAGGCGTCC 
TCCAACCCTA ACAACGTCCT CAAGTTCTGT GACAACAGCA GTGCCATCCA GGGGAAAGAA 
GTCCGATTCT TGCGGCCTAA GGACCCCACA CGGCCAAGCT CCTTCCAGCA ATGTCAGGGG 
CTGAGACATG TTGTCTTCAC AGCAGAGACA CACAACTTCC CTACAGGAGT AGCCCCCTTC 
AGTGGTGCAG CCACTGGCAC AGGGGGCCGG ATCCGAGATG TCCAGTGCAC AGGCCGAGGG 
GCCCACGTGG TGGCTGGCAC TGCTGGCTAT TGCTTTGGAA ACCTGCACAT CCCAGGTTAC 
AGTATGCCCT GGGAGGATCC AAGCTTCCAG TATCCAGGAA ACTTTGCCCG ACCCCTGGAG 
ATTGCCATTG AGGCCAGTAA TGGGGCTTCT GACTACGGCA ACAAGTTTGG GGAACCAGTG 
CTTGCTGGCT TTGCCCGCTC CTTGGGCCTT CAGCTTCCGG ACGGCCAGCG GCGTGAGTGG 
ATCAAGCCCA TAATGTTTAG TGGAGGCATT GGGTCCATGG AAGCTGAGCA TGTGAGCAAG 
GAGCCCCCAG AGACAGGCAT GGATGTCGTG AAGATTGGCG GTCCTGTCTA CAGGATTGGA 
GTTGGGGGCG GAGCTGCTTC GTCTGTGCAG GTGCAAGGAG ACAACGCCAG TGACCTGGAT 
TTTGGGGCTG TGCAGCGGGG AGACCCAGAG ATGGAGCAGA AGATGAACCG TGTGATCCGG 
GCTTGTGTGG AGGCCCCTGG GGGAAACCCC ATCTGCAGCC TCCATGACCA GGGTGCTGGT 
GGCAATGGCA ATGTCCTGAA GGAGCTGAGT GACCCGGCTG GAGCCGTCAT TCACACCAGC 
TGCTTCCAGC TGGGGGACCC AACCCTGAAT GCCCTGGAAA TCTGGGGGGC CGAGTACCAG 
GAGTCGAACG CACTTCTGCT GAGGCCCTCT GACCGGGACT TCCTGAGTTG TGTCAGCGCC 
CGGGAACGCT GCCCAGTCTG CTTTGTGGGC ACCATCACTG GAGACAGGAG AATAGTGCTG 
GTGGATGATC GGGAGTGGCC TATGGGCACA GGTGGCCAGG TGGATGCGCC CCCCACTCCT 
CCCCCAACCC CTGTGGACCT GGACCTGGAC TGGGTGCTGG GCAAGATGCC CCAGAAGGAG 
TTCTTCCTCC AGAGGAGTCT CCCCTTGCTG CAGCCTCTGA CTCTGCCCCC AGGGCTGAGC 
GTGCGCCAGG CTCTGGCGCG GGTCCTCAGG CTGCCTGCCG TGGCCAGCAA GCGCTACCTC 
ACCAACAAGG TGGACCGTTC TGTGGGCGGC CTGGTGGCCC AGCAGCAGTG TGTGGGACCC 
CTGCAGACCC CTCTGGCAGA TGTGGCGGTT GTGGCACTGA GTCACCAGGA GCTTGTAGGG 
GCCGCCACAG CCCTGGGAGA GCAGCCAGTC AAGAGCCTGC TGGACCCGAA GGTTGCCGCC 
CGGCTGGCTG TGGCCGAAGC CCTCACCAAC CTGGTATTTG CTCTGGTCAC CGACCTCCAG 
GATGTGAAAT GCAGCGGGAA CTGGATGTGG GCAGCCAAGC TCCCAGGGGA GGGTGCAGCT 
CTGGCTGACG CCTGTGAGGC TATGGTGGCA GTGATGGCGG CCCTGGGTGT AGCGGTAGAC 
GGTGGCAAGG ACTCCCTCAG CATGGCTGCC CGGGTTGGCT CTGAAACCGT GCGGGCTCCT 
GGGTCACTGG TCATCTCAGC CTATGCTGTC TGTTCAGACA TTACAGCCAC TGTGACCCCA 
GACCTCAAGC GTCCTGGGGG GAGAGGCCAC CTGCTCTACG TGCCTCTGAG TCCTGGGCAG 
CACCGGCTGG GGGGCACAGC TCTGGCTCAG TGCTTCTCCC AGCTTGGCGA GCAGCCTCCC 
GACCTGGATG TTCCTGAGAA CTTGGTGCGA GCCTTCAGCA TCACCCAGGG GCTGCTGAAA 
GACCGCCTCC TCTGCTCAGG CCATGATGTC AGTGATGGAG GTCTCATCAC TTGCCTGCTA 
GAGATGGCCT TTGCTGGGAA TTGTGGGATA GAGGTGGACA TCCCCGCACC TGGGGTCGAT 
GCCCTGCCTG TGCTGTTCGC CGAGGAGCCA GGCCTGGTGC TGGAGGTGCA GGAGCCGGAC 
CTCGCCCAAG TGCTGATGCG TTACCGGAAT GCTGGCCTCC ACTGCCTAGA GCTGGGTCTC 
ACAGGCGACA CGGGGCCCCA CGCCATGGTC CGCGTGTCGG TGAACAGGAC GGTGGTTCTG 
GAGGAACCTG TTGGGCAGCT ACGAGCCCTC TGGGAGGAAA CCAGTTTCCA GTTGGACCGG 
CTACAGGCAA AACCACACTG TGTGGCCCAG GAAGAGCAAG GGCTGAGGGA GCGGGCAGGG 
CCCACCTTCT GCCTGCCGCC TACCTTCCCC AGAGCTTCCG TGCCTTGTGA GCCTGGTGGT 
CCTGCCCCCC GAGTCGCCAT CATTCGGGAA GAGGGCAGTA ATGGAGACCG GGAGATGGCA 
GATGCCTTCC ACTTGGCTGG ATTTGAGGTG TGGGATGTAA CCATGCAGGA CCTGTGCTCT 
GGAGCCATCG GGCTGGACAC ATTCCGCGGT GTGGCCTTCG TAGGCGGCTT CAGCTACGCG 
GACGTCCTGG GCTCTGCCAA AGGCTGGGCA GCTGCTGTGA CCTTTAACCC GCAGGCCGGG 
GCTGAGATGA AGCGCTTCCG CCAGCGGCCA GACACCTTCA GCCTGGGCGT GTGTAACGGC 
TGTCAGCTGC TGGCCCTGCT GGGCTGGGTG GGAGGCAGTT CTAGTGAGGA GGTGGTGGAG 
ATGTGCCAGG AGTCCTGGCC GGCCCGGCCC GGTCTCCTGC TACGCCACAA CCTGTCTGGG 
CGCTTTGAGT CGCGCTGGGC TAGTGTGCGC GTGGGGCCCG GGCCAGCCCT GATGCTGCGA 
GGGATGGAAG GCGCGGTGCT GCCCGTGTGG AGCGCTCACG GGGAAGGTTA CATGGCATTT 
TCTTCTCCGG AACTCCAAGC CCAGATGGAG GCCAGGGGCT TAGCTCCACT GCACTGGGCA 
GATGATGATG GGAACCCCAC GGAACAGTAC CCCCTGAATC CCAACGGCTC CCCAGGGGGT 
GTCGCTGGCA TCTGCTCCCC CGATGGCCGC CACTTGGCCC TCATGCCTCA CCCGGAGCGG 
TCTGTGAGGC CTTGGCAGTG GGCCTGGCGA CCTCCTCCAT TTGACACTCT AACCACCTCC 
CCCTGGCTCC AGCTCTTCAT CAATGCCCGG AATTGGACCC AGGAATGCAG CTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Mapping of the bovine genes of the de novo AMP synthesis pathway.
Animal genetics35(6)438-44(2004 Dec)
B?nsdorff T,Gautier M,Farstad W,R?nningen K,Lingaas F,Olsaker I

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PFAS cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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