Pfas cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pfas
Entrez Gene ID	33847
Full Name	adenosine 2
Synonyms	153122_at,Ade2,CG9127,Dmel\CG9127,Paics,pym
General protein information	Preferred Names adenosine 2 Names CG9127 gene product from transcript CG9127-RB CG9127-PA CG9127-PB CG9127-PC ade2-PA ade2-PB ade2-PC adenosine2
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 26B3-26B3\|2-17.7 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_057864.4	NP_477212.1	phosphoribosylformylglycinamidine synthase, isoform A
NM_164675.2	NP_723146.1	phosphoribosylformylglycinamidine synthase, isoform B
NM_164676.2	NP_723147.1	phosphoribosylformylglycinamidine synthase, isoform C

Pathways from BioSystems

Homologies

	PFAS	XP_001112405.1
Pan troglodytes (chimpanzee)	PFAS	XP_511854.3
Bos taurus (cattle)	PFAS	NP_001243493.1
Mus musculus (house mouse)	Pfas	NP_001152991.1
Drosophila melanogaster (fruit fly)	ade2	NP_723146.1
Caenorhabditis elegans (roundworm)	CELE_F10F2.2	NP_497942.2
Homo sapiens (human)	PFAS	NP_036525.1
Canis lupus familiaris (dog)	PFAS	XP_005620078.1
Rattus norvegicus (Norway rat)	Pfas	NP_001099261.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002091	XP_320948.2
Arabidopsis thaliana (thale cress)	PUR4	NP_177566.3
Danio rerio (zebrafish)	pfas	NP_001038667.1
Oryza sativa (rice)	Os01g0888500	NP_001045039.1
Xenopus tropicalis (tropical clawed frog)	pfas	NP_001135692.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

together with the fact that the EJC-disassembly activity of DmPYM is regulated, implicates PYM as an effector of EJC homeostasis in vivo.
Title: The EJC binding and dissociating activity of PYM is regulated in Drosophila.

Data show the presence of apoptosis at the cellular level in both ade2 and Prat mutants, and the upregulated gene HtrA2, which encodes an apoptosis effector and is thus a candidate for initiating apoptosis in response to purine depletion.
Title: A link between impaired purine nucleotide synthesis and apoptosis in Drosophila melanogaster.

The following Pfas gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pfas cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa13865	NM_164676.2 Latest version!	Drosophila melanogaster phosphoribosylformylglycinamidine synthase, transcript variant C (Pfas), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa13865	NM_057864.4 Latest version!	Drosophila melanogaster phosphoribosylformylglycinamidine synthase, transcript variant A (Pfas), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa13865	NM_164675.2 Latest version!	Drosophila melanogaster phosphoribosylformylglycinamidine synthase, transcript variant B (Pfas), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pfas ( NM_057864.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477212.1 Drosophila melanogaster phosphoribosylformylglycinamidine synthase, transcript variant A (Pfas), mRNA.

Price & Availability↑

CloneID	OFa13865
Clone ID Related Accession (Same CDS sequence)	NM_057864.4 , NM_164675.2 , NM_164676.2
Accession Version	NM_057864.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	phosphoribosylformylglycinamidine synthase, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_057864.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTCATCC TTCGCTACTA CGATGTGCAG GCCCACTCCG CGGCCGAGGA GGAGAGTGTC 
CTGCGTCGGT TGCGCGAGGA AGACGGCGCC GTGGTGTCCG TGCGCATGGA GCGCTGCTAT 
CATCTGGAGT ACAGCGCTCA GGCAGAGCAC TCACTGGCCC TGGACGAGCT GCTGGTGTGG 
CTGGTCAAGC AACCGCTGAG CAAAGGCCAG AGCTTGTCCA GGCAACCTGC CCTGCAGTCA 
ACTGGGTCGA GTCAGTTGCT CCTGGAGATC GGGCCGCGTT TTAACTTCTC CACGCCGTAC 
TCCACGAACT GCGTGAACAT ATTCCAGAAT CTCGGGTACT CAGAGGTGCG TCGCATGGAA 
ACCTCCACCC GCTATCTGGT TACTTTTGGC GAGGGATCAA AGGCGCCGGA GGCAGCCAGG 
TTTGTTCCTC TGCTCGGTGA CCGCATGACC CAGTGCTTGT ACACCGAGGA GAATACCCCC 
AAGGCGAGCT TTGACGAGCA GCTACCTGAG CGCCAGGCCA ACTGGCATTT CGTGCCCGTT 
TTGGAGGAGG GTAGGGCGGC ACTGGAGCGG ATTAATCAGG AGCTGGGCTT AGCCTTCAAC 
GACTACGATT TGGACTACTA CCACGACTTG TTTGCCAAGG AGCTGGGCCG CAATCCCACC 
ACTGTGGAGC TCTTCGATTG CGCCCAGAGC AACAGTGAGC ACTCGCGCCA CTGGTTTTTC 
CGCGGACGTA TGGTGATCGA CGGCGTGGAG CAGCCCAAGT CGCTGATTCG CATGATCATG 
GACACGCAGG CCCACACGAA CCCGAACAAC ACCATTAAGT TCAGCGACAA CAGCAGTGCC 
ATGGTGGGAT TCGATCACCA GACCATAGTT CCGTCCTCCG TGGTCGCTCC CGGCGCAGTG 
CGTCTGCAGA GCGTGCAGTC TGACCTGATT TTCACGGCGG AGACCCACAA CATGCCCACT 
GCAGTGGCGC CTTTCAGCGG AGCCACCACC GGCACTGGCG GACGACTGCG TGATGTCCAG 
GGCGTGGGCA GAGGAGGCGT GCCGATCGCC GGCACCGCTG GCTACTGTGT TGGCGCTCTT 
CACATTCCAG GTTACAAACA GCCGTACGAG CCTTTGGACT TTAAATACCC TGCGACGTTT 
GCGCCCCCAC TTCAGGTGCT CATTGAGGCG AGCAATGGCG CCTCCGACTA CGGAAATAAG 
TTCGGCGAGC CAGTGATCTC TGGTTTTGCC CTCTCCTATG GACTGAACAG TGCTGCCGAT 
GCCAGCCAGC GGGATGAGTA CGTGAAACCG ATCATGTTCA GCGGTGGCTT GGGCACCATG 
CCCGCTACCA TGCGCGAGAA GCTGCCGCCG GCACGCGGTC AGTTGCTGGC CAAGATCGGG 
GGCCCAGTGT ACAGGATTGG AGTGGGTGGC GGCGCCGCAA GCTCTGTGGA GATACAAGGA 
TCCGGAGATG CTGAGCTAGA TTTTAATGCC GTACAGCGAG GAGACGCCGA AATGGAGAAT 
AAATTGAACC GTGTGGTGCG AGCCTGTCTA GACCTGGGCG AGCAAAACCC CATATTAGCC 
ATCCATGACC AGGGGGCTGG CGGCAACGGC AATGTCCTCA AGGAGCTAGT GGAACCAGGC 
TTTGCCGGAG CTGTTATCTT CTCCAAGGAG TTCCAACTCG GCGATCCCAC GATCACTGCC 
CTCGAACTGT GGGGTGCCGA GTACCAGGAA AACAATGCCA TTCTTTGCAA CGCTGATCAA 
CGCGAACTGC TGGAAAAGAT CTGCCGCCGT GAACGCTGTC CCATCAGCTT CGTGGGAGTG 
GTGACCGGAG ATGGGCGAGT AACGCTGCTG GAGAAGCCTG CCCCCAAGGA TCTGGAACAG 
GCGTTGAATG CGTCCAATCG CAGCGAAGTC TCACCGTTCG ACTTGGAACT TAAGTACGTT 
TTGGGCGACA TGCCCAAGAG AACGTACGAC TTGAAACGCG AACAGACGCC GCTCAAGGAG 
TTGAGTCTGC CGAAAGGTCT CCTTCTGGAC GAGGCCTTGG AGAGAGTTCT TAGCTTGGTG 
GCAGTCGGTA GCAAGCGCTT CCTGACCAAC AAGGTGGATC GCTGCGTCGG TGGATTGATT 
GCACAGCAAC AGTGCGTGGG ACCGCTCCAA GCTCCACTAG CGGACTACGC GTTAACCACC 
GTCTCCCACT TTAGTCACTC TGGAATCGCC ACTTCAATTG GTACACAGCC ACTAAAAGGA 
CTGCTTGATC CCGCCGCAAT GGCCAGGATG TGTGTGGCTG AAGCGTTGAG TAACCTTGTG 
TTTGTAAAGA TCAGCGAACT GGCTGATGTC AAGTGCTCTG GCAACTGGAT GTGGGCTGCA 
AAGCTGCCCG GCGAGGGTGC CCGGATGTTC GACGCCTGCA AGGAGCTCTG TCAGATTCTT 
GAGGAACTTC ACATCGCCAT CGATGGCGGA AAGGACTCCC TGTCCATGGC GGCTAAGGTG 
GGCGGTGAGA CAATAAAGTC CCCGGGTACT CTTGTCATCT CCACCTATGC TCCCTGTCCG 
GATGTGCGGC TAAAGGTAAC GCCTGATCTT AAAGGACCCG GCGCGGGGTC GAAGACTTCC 
CTGCTCTGGA TAAATCTGGA GAACAGCGCT CGCCTAGGTG GATCGGCTCT GGCTCAGGCA 
TACGCGCAGC AGGGCAAAGA TACGCCAAAC TTGACAAGGA GTGATGTGCT GGGCAAAGCA 
TTTGCTGTGA CTCAATCGTT GTTGGGAGAT GGACTTATTC AAGCAGGACA CGATGTCAGC 
GACGGAGGAT TGCTTGTTTG CGTATTGGAG ATGGCCATCG GAGGACTGAG TGGCCTTAGA 
GTGGATCTAT CGGAGCCCTT GGCCAAGCTC AAGAACTTCG ATAAGTCTGT CGAGAAACTT 
AATCGTCCCG AACTAGCTGT TCTCTTCGCC GAGGAGTGTG GATGGGTGGT GGAAGTCCTG 
GACACAGACC TGGAACGAGT TCGATCTACC TACGAAAAGG CAGGAGTACC AAACTACTAT 
TTGGGCGTGA CCGAGGGATT CGGACTGGAT TCCAGAGTGG TACTCAAGAA CGGCAAGTCA 
GAGCTGCTGG ACCAGCCACT CCGTGTGCTC TACAAGAAAT GGGAGAGAAC CAGTTACGAG 
CTGGAAAAGC TTCAGGCCAA CCCTGAGTGC GCCGAGGCAG AGTACAATAG CCTTGAGTAT 
CGCCAAGCAC CTCAGTATAG GGGACCTCAG AATGTGCAGG CCGAGCTTAC TCTCAAGCGA 
TCCTCCGCCC CAGTTCGCGT GGCCGTGCTC CGTGAGGAGG GTGTCAATAG TGAGCGCGAG 
ATGATGGCCT GCCTGCTGAG GGCTAACTTC GAGGTGCACG ATGTGACCAT GTCGGATCTT 
CTTCAAGGCA CCGCAAGCGT GTCCCAATAC CGCGGACTAA TTTTCCCGGG CGGCTTCAGC 
TATGCGGACA CTTTGGGATC CGCCAAGGGC TGGGCAGCAA ACATCCTCCA CAATCCCCGC 
TTGTTGCCTC AGTTTGAGGC TTTCAAGCGT CGTCAAGATG TGTTCTCCTT GGGCATTTGC 
AATGGTTGCC AGCTGATGAC ACTTATAGGA TTTGTTGGCA GTGCAAAGAG CGAGGTGGGC 
GCAGATCCCG ACGTGGCCCT GCTCCATAAC AAATCGCAGC GGTTCGAGTG TCGTTGGGCC 
ACCGTGAAGA TTCCAAGCAA TCGCTCCATC ATGCTCGGAA GTATGAAGGA TCTGGTTCTT 
GGCTGTTGGG TGGCTCACGG CGAAGGTCGC TTTGCATTCC GCGACGAGAA GCTCATTAGC 
CACTTGCAGT CGGAACAGCT GGTGACATTG CAATATGTTG ATGATGTAGG CAAACCGACG 
GAACTGTATC CACTTAACCC GAACGGTAGT CCTCAGGGAA TTGCTGGCCT TTGCTCGTCG 
GATGGACGTC ACTTGGCCCT CATGCCACAT CCCGAGCGAT GCTCCTCCAT GTACCAGTGG 
CCCTATGTGC CATCTTCATT CGAAGTTTCC CCCACACAAT CGGAAAGTCC TTGGCAGATT 
ATGTTTAACA ACGCGTACAA TTGGTGTGTC AAGTCGAATC AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477212.1
CDS	84..4148
Translation	MVILRYYDVQAHSAAEEESVLRRLREEDGAVVSVRMERCYHLEYSAQAEHSLALDELLVWLVKQPLSKGQSLSRQPALQSTGSSQLLLEIGPRFNFSTPYSTNCVNIFQNLGYSEVRRMETSTRYLVTFGEGSKAPEAARFVPLLGDRMTQCLYTEENTPKASFDEQLPERQANWHFVPVLEEGRAALERINQELGLAFNDYDLDYYHDLFAKELGRNPTTVELFDCAQSNSEHSRHWFFRGRMVIDGVEQPKSLIRMIMDTQAHTNPNNTIKFSDNSSAMVGFDHQTIVPSSVVAPGAVRLQSVQSDLIFTAETHNMPTAVAPFSGATTGTGGRLRDVQGVGRGGVPIAGTAGYCVGALHIPGYKQPYEPLDFKYPATFAPPLQVLIEASNGASDYGNKFGEPVISGFALSYGLNSAADASQRDEYVKPIMFSGGLGTMPATMREKLPPARGQLLAKIGGPVYRIGVGGGAASSVEIQGSGDAELDFNAVQRGDAEMENKLNRVVRACLDLGEQNPILAIHDQGAGGNGNVLKELVEPGFAGAVIFSKEFQLGDPTITALELWGAEYQENNAILCNADQRELLEKICRRERCPISFVGVVTGDGRVTLLEKPAPKDLEQALNASNRSEVSPFDLELKYVLGDMPKRTYDLKREQTPLKELSLPKGLLLDEALERVLSLVAVGSKRFLTNKVDRCVGGLIAQQQCVGPLQAPLADYALTTVSHFSHSGIATSIGTQPLKGLLDPAAMARMCVAEALSNLVFVKISELADVKCSGNWMWAAKLPGEGARMFDACKELCQILEELHIAIDGGKDSLSMAAKVGGETIKSPGTLVISTYAPCPDVRLKVTPDLKGPGAGSKTSLLWINLENSARLGGSALAQAYAQQGKDTPNLTRSDVLGKAFAVTQSLLGDGLIQAGHDVSDGGLLVCVLEMAIGGLSGLRVDLSEPLAKLKNFDKSVEKLNRPELAVLFAEECGWVVEVLDTDLERVRSTYEKAGVPNYYLGVTEGFGLDSRVVLKNGKSELLDQPLRVLYKKWERTSYELEKLQANPECAEAEYNSLEYRQAPQYRGPQNVQAELTLKRSSAPVRVAVLREEGVNSEREMMACLLRANFEVHDVTMSDLLQGTASVSQYRGLIFPGGFSYADTLGSAKGWAANILHNPRLLPQFEAFKRRQDVFSLGICNGCQLMTLIGFVGSAKSEVGADPDVALLHNKSQRFECRWATVKIPSNRSIMLGSMKDLVLGCWVAHGEGRFAFRDEKLISHLQSEQLVTLQYVDDVGKPTELYPLNPNGSPQGIAGLCSSDGRHLALMPHPERCSSMYQWPYVPSSFEVSPTQSESPWQIMFNNAYNWCVKSNQ

Target ORF information:

RefSeq Version	NM_057864.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster phosphoribosylformylglycinamidine synthase, transcript variant A (Pfas), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057864.4

ORF Insert Sequence:

ATGGTCATCC TTCGCTACTA CGATGTGCAG GCCCACTCCG CGGCCGAGGA GGAGAGTGTC 
CTGCGTCGGT TGCGCGAGGA AGACGGCGCC GTGGTGTCCG TGCGCATGGA GCGCTGCTAT 
CATCTGGAGT ACAGCGCTCA GGCAGAGCAC TCACTGGCCC TGGACGAGCT GCTGGTGTGG 
CTGGTCAAGC AACCGCTGAG CAAAGGCCAG AGCTTGTCCA GGCAACCTGC CCTGCAGTCA 
ACTGGGTCGA GTCAGTTGCT CCTGGAGATC GGGCCGCGTT TTAACTTCTC CACGCCGTAC 
TCCACGAACT GCGTGAACAT ATTCCAGAAT CTCGGGTACT CAGAGGTGCG TCGCATGGAA 
ACCTCCACCC GCTATCTGGT TACTTTTGGC GAGGGATCAA AGGCGCCGGA GGCAGCCAGG 
TTTGTTCCTC TGCTCGGTGA CCGCATGACC CAGTGCTTGT ACACCGAGGA GAATACCCCC 
AAGGCGAGCT TTGACGAGCA GCTACCTGAG CGCCAGGCCA ACTGGCATTT CGTGCCCGTT 
TTGGAGGAGG GTAGGGCGGC ACTGGAGCGG ATTAATCAGG AGCTGGGCTT AGCCTTCAAC 
GACTACGATT TGGACTACTA CCACGACTTG TTTGCCAAGG AGCTGGGCCG CAATCCCACC 
ACTGTGGAGC TCTTCGATTG CGCCCAGAGC AACAGTGAGC ACTCGCGCCA CTGGTTTTTC 
CGCGGACGTA TGGTGATCGA CGGCGTGGAG CAGCCCAAGT CGCTGATTCG CATGATCATG 
GACACGCAGG CCCACACGAA CCCGAACAAC ACCATTAAGT TCAGCGACAA CAGCAGTGCC 
ATGGTGGGAT TCGATCACCA GACCATAGTT CCGTCCTCCG TGGTCGCTCC CGGCGCAGTG 
CGTCTGCAGA GCGTGCAGTC TGACCTGATT TTCACGGCGG AGACCCACAA CATGCCCACT 
GCAGTGGCGC CTTTCAGCGG AGCCACCACC GGCACTGGCG GACGACTGCG TGATGTCCAG 
GGCGTGGGCA GAGGAGGCGT GCCGATCGCC GGCACCGCTG GCTACTGTGT TGGCGCTCTT 
CACATTCCAG GTTACAAACA GCCGTACGAG CCTTTGGACT TTAAATACCC TGCGACGTTT 
GCGCCCCCAC TTCAGGTGCT CATTGAGGCG AGCAATGGCG CCTCCGACTA CGGAAATAAG 
TTCGGCGAGC CAGTGATCTC TGGTTTTGCC CTCTCCTATG GACTGAACAG TGCTGCCGAT 
GCCAGCCAGC GGGATGAGTA CGTGAAACCG ATCATGTTCA GCGGTGGCTT GGGCACCATG 
CCCGCTACCA TGCGCGAGAA GCTGCCGCCG GCACGCGGTC AGTTGCTGGC CAAGATCGGG 
GGCCCAGTGT ACAGGATTGG AGTGGGTGGC GGCGCCGCAA GCTCTGTGGA GATACAAGGA 
TCCGGAGATG CTGAGCTAGA TTTTAATGCC GTACAGCGAG GAGACGCCGA AATGGAGAAT 
AAATTGAACC GTGTGGTGCG AGCCTGTCTA GACCTGGGCG AGCAAAACCC CATATTAGCC 
ATCCATGACC AGGGGGCTGG CGGCAACGGC AATGTCCTCA AGGAGCTAGT GGAACCAGGC 
TTTGCCGGAG CTGTTATCTT CTCCAAGGAG TTCCAACTCG GCGATCCCAC GATCACTGCC 
CTCGAACTGT GGGGTGCCGA GTACCAGGAA AACAATGCCA TTCTTTGCAA CGCTGATCAA 
CGCGAACTGC TGGAAAAGAT CTGCCGCCGT GAACGCTGTC CCATCAGCTT CGTGGGAGTG 
GTGACCGGAG ATGGGCGAGT AACGCTGCTG GAGAAGCCTG CCCCCAAGGA TCTGGAACAG 
GCGTTGAATG CGTCCAATCG CAGCGAAGTC TCACCGTTCG ACTTGGAACT TAAGTACGTT 
TTGGGCGACA TGCCCAAGAG AACGTACGAC TTGAAACGCG AACAGACGCC GCTCAAGGAG 
TTGAGTCTGC CGAAAGGTCT CCTTCTGGAC GAGGCCTTGG AGAGAGTTCT TAGCTTGGTG 
GCAGTCGGTA GCAAGCGCTT CCTGACCAAC AAGGTGGATC GCTGCGTCGG TGGATTGATT 
GCACAGCAAC AGTGCGTGGG ACCGCTCCAA GCTCCACTAG CGGACTACGC GTTAACCACC 
GTCTCCCACT TTAGTCACTC TGGAATCGCC ACTTCAATTG GTACACAGCC ACTAAAAGGA 
CTGCTTGATC CCGCCGCAAT GGCCAGGATG TGTGTGGCTG AAGCGTTGAG TAACCTTGTG 
TTTGTAAAGA TCAGCGAACT GGCTGATGTC AAGTGCTCTG GCAACTGGAT GTGGGCTGCA 
AAGCTGCCCG GCGAGGGTGC CCGGATGTTC GACGCCTGCA AGGAGCTCTG TCAGATTCTT 
GAGGAACTTC ACATCGCCAT CGATGGCGGA AAGGACTCCC TGTCCATGGC GGCTAAGGTG 
GGCGGTGAGA CAATAAAGTC CCCGGGTACT CTTGTCATCT CCACCTATGC TCCCTGTCCG 
GATGTGCGGC TAAAGGTAAC GCCTGATCTT AAAGGACCCG GCGCGGGGTC GAAGACTTCC 
CTGCTCTGGA TAAATCTGGA GAACAGCGCT CGCCTAGGTG GATCGGCTCT GGCTCAGGCA 
TACGCGCAGC AGGGCAAAGA TACGCCAAAC TTGACAAGGA GTGATGTGCT GGGCAAAGCA 
TTTGCTGTGA CTCAATCGTT GTTGGGAGAT GGACTTATTC AAGCAGGACA CGATGTCAGC 
GACGGAGGAT TGCTTGTTTG CGTATTGGAG ATGGCCATCG GAGGACTGAG TGGCCTTAGA 
GTGGATCTAT CGGAGCCCTT GGCCAAGCTC AAGAACTTCG ATAAGTCTGT CGAGAAACTT 
AATCGTCCCG AACTAGCTGT TCTCTTCGCC GAGGAGTGTG GATGGGTGGT GGAAGTCCTG 
GACACAGACC TGGAACGAGT TCGATCTACC TACGAAAAGG CAGGAGTACC AAACTACTAT 
TTGGGCGTGA CCGAGGGATT CGGACTGGAT TCCAGAGTGG TACTCAAGAA CGGCAAGTCA 
GAGCTGCTGG ACCAGCCACT CCGTGTGCTC TACAAGAAAT GGGAGAGAAC CAGTTACGAG 
CTGGAAAAGC TTCAGGCCAA CCCTGAGTGC GCCGAGGCAG AGTACAATAG CCTTGAGTAT 
CGCCAAGCAC CTCAGTATAG GGGACCTCAG AATGTGCAGG CCGAGCTTAC TCTCAAGCGA 
TCCTCCGCCC CAGTTCGCGT GGCCGTGCTC CGTGAGGAGG GTGTCAATAG TGAGCGCGAG 
ATGATGGCCT GCCTGCTGAG GGCTAACTTC GAGGTGCACG ATGTGACCAT GTCGGATCTT 
CTTCAAGGCA CCGCAAGCGT GTCCCAATAC CGCGGACTAA TTTTCCCGGG CGGCTTCAGC 
TATGCGGACA CTTTGGGATC CGCCAAGGGC TGGGCAGCAA ACATCCTCCA CAATCCCCGC 
TTGTTGCCTC AGTTTGAGGC TTTCAAGCGT CGTCAAGATG TGTTCTCCTT GGGCATTTGC 
AATGGTTGCC AGCTGATGAC ACTTATAGGA TTTGTTGGCA GTGCAAAGAG CGAGGTGGGC 
GCAGATCCCG ACGTGGCCCT GCTCCATAAC AAATCGCAGC GGTTCGAGTG TCGTTGGGCC 
ACCGTGAAGA TTCCAAGCAA TCGCTCCATC ATGCTCGGAA GTATGAAGGA TCTGGTTCTT 
GGCTGTTGGG TGGCTCACGG CGAAGGTCGC TTTGCATTCC GCGACGAGAA GCTCATTAGC 
CACTTGCAGT CGGAACAGCT GGTGACATTG CAATATGTTG ATGATGTAGG CAAACCGACG 
GAACTGTATC CACTTAACCC GAACGGTAGT CCTCAGGGAA TTGCTGGCCT TTGCTCGTCG 
GATGGACGTC ACTTGGCCCT CATGCCACAT CCCGAGCGAT GCTCCTCCAT GTACCAGTGG 
CCCTATGTGC CATCTTCATT CGAAGTTTCC CCCACACAAT CGGAAAGTCC TTGGCAGATT 
ATGTTTAACA ACGCGTACAA TTGGTGTGTC AAGTCGAATC AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pfas ( NM_164675.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723146.1 Drosophila melanogaster phosphoribosylformylglycinamidine synthase, transcript variant B (Pfas), mRNA.

Price & Availability↑

CloneID	OFa13865
Clone ID Related Accession (Same CDS sequence)	NM_057864.4 , NM_164675.2 , NM_164676.2
Accession Version	NM_164675.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	phosphoribosylformylglycinamidine synthase, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_164675.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTCATCC TTCGCTACTA CGATGTGCAG GCCCACTCCG CGGCCGAGGA GGAGAGTGTC 
CTGCGTCGGT TGCGCGAGGA AGACGGCGCC GTGGTGTCCG TGCGCATGGA GCGCTGCTAT 
CATCTGGAGT ACAGCGCTCA GGCAGAGCAC TCACTGGCCC TGGACGAGCT GCTGGTGTGG 
CTGGTCAAGC AACCGCTGAG CAAAGGCCAG AGCTTGTCCA GGCAACCTGC CCTGCAGTCA 
ACTGGGTCGA GTCAGTTGCT CCTGGAGATC GGGCCGCGTT TTAACTTCTC CACGCCGTAC 
TCCACGAACT GCGTGAACAT ATTCCAGAAT CTCGGGTACT CAGAGGTGCG TCGCATGGAA 
ACCTCCACCC GCTATCTGGT TACTTTTGGC GAGGGATCAA AGGCGCCGGA GGCAGCCAGG 
TTTGTTCCTC TGCTCGGTGA CCGCATGACC CAGTGCTTGT ACACCGAGGA GAATACCCCC 
AAGGCGAGCT TTGACGAGCA GCTACCTGAG CGCCAGGCCA ACTGGCATTT CGTGCCCGTT 
TTGGAGGAGG GTAGGGCGGC ACTGGAGCGG ATTAATCAGG AGCTGGGCTT AGCCTTCAAC 
GACTACGATT TGGACTACTA CCACGACTTG TTTGCCAAGG AGCTGGGCCG CAATCCCACC 
ACTGTGGAGC TCTTCGATTG CGCCCAGAGC AACAGTGAGC ACTCGCGCCA CTGGTTTTTC 
CGCGGACGTA TGGTGATCGA CGGCGTGGAG CAGCCCAAGT CGCTGATTCG CATGATCATG 
GACACGCAGG CCCACACGAA CCCGAACAAC ACCATTAAGT TCAGCGACAA CAGCAGTGCC 
ATGGTGGGAT TCGATCACCA GACCATAGTT CCGTCCTCCG TGGTCGCTCC CGGCGCAGTG 
CGTCTGCAGA GCGTGCAGTC TGACCTGATT TTCACGGCGG AGACCCACAA CATGCCCACT 
GCAGTGGCGC CTTTCAGCGG AGCCACCACC GGCACTGGCG GACGACTGCG TGATGTCCAG 
GGCGTGGGCA GAGGAGGCGT GCCGATCGCC GGCACCGCTG GCTACTGTGT TGGCGCTCTT 
CACATTCCAG GTTACAAACA GCCGTACGAG CCTTTGGACT TTAAATACCC TGCGACGTTT 
GCGCCCCCAC TTCAGGTGCT CATTGAGGCG AGCAATGGCG CCTCCGACTA CGGAAATAAG 
TTCGGCGAGC CAGTGATCTC TGGTTTTGCC CTCTCCTATG GACTGAACAG TGCTGCCGAT 
GCCAGCCAGC GGGATGAGTA CGTGAAACCG ATCATGTTCA GCGGTGGCTT GGGCACCATG 
CCCGCTACCA TGCGCGAGAA GCTGCCGCCG GCACGCGGTC AGTTGCTGGC CAAGATCGGG 
GGCCCAGTGT ACAGGATTGG AGTGGGTGGC GGCGCCGCAA GCTCTGTGGA GATACAAGGA 
TCCGGAGATG CTGAGCTAGA TTTTAATGCC GTACAGCGAG GAGACGCCGA AATGGAGAAT 
AAATTGAACC GTGTGGTGCG AGCCTGTCTA GACCTGGGCG AGCAAAACCC CATATTAGCC 
ATCCATGACC AGGGGGCTGG CGGCAACGGC AATGTCCTCA AGGAGCTAGT GGAACCAGGC 
TTTGCCGGAG CTGTTATCTT CTCCAAGGAG TTCCAACTCG GCGATCCCAC GATCACTGCC 
CTCGAACTGT GGGGTGCCGA GTACCAGGAA AACAATGCCA TTCTTTGCAA CGCTGATCAA 
CGCGAACTGC TGGAAAAGAT CTGCCGCCGT GAACGCTGTC CCATCAGCTT CGTGGGAGTG 
GTGACCGGAG ATGGGCGAGT AACGCTGCTG GAGAAGCCTG CCCCCAAGGA TCTGGAACAG 
GCGTTGAATG CGTCCAATCG CAGCGAAGTC TCACCGTTCG ACTTGGAACT TAAGTACGTT 
TTGGGCGACA TGCCCAAGAG AACGTACGAC TTGAAACGCG AACAGACGCC GCTCAAGGAG 
TTGAGTCTGC CGAAAGGTCT CCTTCTGGAC GAGGCCTTGG AGAGAGTTCT TAGCTTGGTG 
GCAGTCGGTA GCAAGCGCTT CCTGACCAAC AAGGTGGATC GCTGCGTCGG TGGATTGATT 
GCACAGCAAC AGTGCGTGGG ACCGCTCCAA GCTCCACTAG CGGACTACGC GTTAACCACC 
GTCTCCCACT TTAGTCACTC TGGAATCGCC ACTTCAATTG GTACACAGCC ACTAAAAGGA 
CTGCTTGATC CCGCCGCAAT GGCCAGGATG TGTGTGGCTG AAGCGTTGAG TAACCTTGTG 
TTTGTAAAGA TCAGCGAACT GGCTGATGTC AAGTGCTCTG GCAACTGGAT GTGGGCTGCA 
AAGCTGCCCG GCGAGGGTGC CCGGATGTTC GACGCCTGCA AGGAGCTCTG TCAGATTCTT 
GAGGAACTTC ACATCGCCAT CGATGGCGGA AAGGACTCCC TGTCCATGGC GGCTAAGGTG 
GGCGGTGAGA CAATAAAGTC CCCGGGTACT CTTGTCATCT CCACCTATGC TCCCTGTCCG 
GATGTGCGGC TAAAGGTAAC GCCTGATCTT AAAGGACCCG GCGCGGGGTC GAAGACTTCC 
CTGCTCTGGA TAAATCTGGA GAACAGCGCT CGCCTAGGTG GATCGGCTCT GGCTCAGGCA 
TACGCGCAGC AGGGCAAAGA TACGCCAAAC TTGACAAGGA GTGATGTGCT GGGCAAAGCA 
TTTGCTGTGA CTCAATCGTT GTTGGGAGAT GGACTTATTC AAGCAGGACA CGATGTCAGC 
GACGGAGGAT TGCTTGTTTG CGTATTGGAG ATGGCCATCG GAGGACTGAG TGGCCTTAGA 
GTGGATCTAT CGGAGCCCTT GGCCAAGCTC AAGAACTTCG ATAAGTCTGT CGAGAAACTT 
AATCGTCCCG AACTAGCTGT TCTCTTCGCC GAGGAGTGTG GATGGGTGGT GGAAGTCCTG 
GACACAGACC TGGAACGAGT TCGATCTACC TACGAAAAGG CAGGAGTACC AAACTACTAT 
TTGGGCGTGA CCGAGGGATT CGGACTGGAT TCCAGAGTGG TACTCAAGAA CGGCAAGTCA 
GAGCTGCTGG ACCAGCCACT CCGTGTGCTC TACAAGAAAT GGGAGAGAAC CAGTTACGAG 
CTGGAAAAGC TTCAGGCCAA CCCTGAGTGC GCCGAGGCAG AGTACAATAG CCTTGAGTAT 
CGCCAAGCAC CTCAGTATAG GGGACCTCAG AATGTGCAGG CCGAGCTTAC TCTCAAGCGA 
TCCTCCGCCC CAGTTCGCGT GGCCGTGCTC CGTGAGGAGG GTGTCAATAG TGAGCGCGAG 
ATGATGGCCT GCCTGCTGAG GGCTAACTTC GAGGTGCACG ATGTGACCAT GTCGGATCTT 
CTTCAAGGCA CCGCAAGCGT GTCCCAATAC CGCGGACTAA TTTTCCCGGG CGGCTTCAGC 
TATGCGGACA CTTTGGGATC CGCCAAGGGC TGGGCAGCAA ACATCCTCCA CAATCCCCGC 
TTGTTGCCTC AGTTTGAGGC TTTCAAGCGT CGTCAAGATG TGTTCTCCTT GGGCATTTGC 
AATGGTTGCC AGCTGATGAC ACTTATAGGA TTTGTTGGCA GTGCAAAGAG CGAGGTGGGC 
GCAGATCCCG ACGTGGCCCT GCTCCATAAC AAATCGCAGC GGTTCGAGTG TCGTTGGGCC 
ACCGTGAAGA TTCCAAGCAA TCGCTCCATC ATGCTCGGAA GTATGAAGGA TCTGGTTCTT 
GGCTGTTGGG TGGCTCACGG CGAAGGTCGC TTTGCATTCC GCGACGAGAA GCTCATTAGC 
CACTTGCAGT CGGAACAGCT GGTGACATTG CAATATGTTG ATGATGTAGG CAAACCGACG 
GAACTGTATC CACTTAACCC GAACGGTAGT CCTCAGGGAA TTGCTGGCCT TTGCTCGTCG 
GATGGACGTC ACTTGGCCCT CATGCCACAT CCCGAGCGAT GCTCCTCCAT GTACCAGTGG 
CCCTATGTGC CATCTTCATT CGAAGTTTCC CCCACACAAT CGGAAAGTCC TTGGCAGATT 
ATGTTTAACA ACGCGTACAA TTGGTGTGTC AAGTCGAATC AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723146.1
CDS	140..4204
Translation	MVILRYYDVQAHSAAEEESVLRRLREEDGAVVSVRMERCYHLEYSAQAEHSLALDELLVWLVKQPLSKGQSLSRQPALQSTGSSQLLLEIGPRFNFSTPYSTNCVNIFQNLGYSEVRRMETSTRYLVTFGEGSKAPEAARFVPLLGDRMTQCLYTEENTPKASFDEQLPERQANWHFVPVLEEGRAALERINQELGLAFNDYDLDYYHDLFAKELGRNPTTVELFDCAQSNSEHSRHWFFRGRMVIDGVEQPKSLIRMIMDTQAHTNPNNTIKFSDNSSAMVGFDHQTIVPSSVVAPGAVRLQSVQSDLIFTAETHNMPTAVAPFSGATTGTGGRLRDVQGVGRGGVPIAGTAGYCVGALHIPGYKQPYEPLDFKYPATFAPPLQVLIEASNGASDYGNKFGEPVISGFALSYGLNSAADASQRDEYVKPIMFSGGLGTMPATMREKLPPARGQLLAKIGGPVYRIGVGGGAASSVEIQGSGDAELDFNAVQRGDAEMENKLNRVVRACLDLGEQNPILAIHDQGAGGNGNVLKELVEPGFAGAVIFSKEFQLGDPTITALELWGAEYQENNAILCNADQRELLEKICRRERCPISFVGVVTGDGRVTLLEKPAPKDLEQALNASNRSEVSPFDLELKYVLGDMPKRTYDLKREQTPLKELSLPKGLLLDEALERVLSLVAVGSKRFLTNKVDRCVGGLIAQQQCVGPLQAPLADYALTTVSHFSHSGIATSIGTQPLKGLLDPAAMARMCVAEALSNLVFVKISELADVKCSGNWMWAAKLPGEGARMFDACKELCQILEELHIAIDGGKDSLSMAAKVGGETIKSPGTLVISTYAPCPDVRLKVTPDLKGPGAGSKTSLLWINLENSARLGGSALAQAYAQQGKDTPNLTRSDVLGKAFAVTQSLLGDGLIQAGHDVSDGGLLVCVLEMAIGGLSGLRVDLSEPLAKLKNFDKSVEKLNRPELAVLFAEECGWVVEVLDTDLERVRSTYEKAGVPNYYLGVTEGFGLDSRVVLKNGKSELLDQPLRVLYKKWERTSYELEKLQANPECAEAEYNSLEYRQAPQYRGPQNVQAELTLKRSSAPVRVAVLREEGVNSEREMMACLLRANFEVHDVTMSDLLQGTASVSQYRGLIFPGGFSYADTLGSAKGWAANILHNPRLLPQFEAFKRRQDVFSLGICNGCQLMTLIGFVGSAKSEVGADPDVALLHNKSQRFECRWATVKIPSNRSIMLGSMKDLVLGCWVAHGEGRFAFRDEKLISHLQSEQLVTLQYVDDVGKPTELYPLNPNGSPQGIAGLCSSDGRHLALMPHPERCSSMYQWPYVPSSFEVSPTQSESPWQIMFNNAYNWCVKSNQ

Target ORF information:

RefSeq Version	NM_164675.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster phosphoribosylformylglycinamidine synthase, transcript variant B (Pfas), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164675.2

ORF Insert Sequence:

ATGGTCATCC TTCGCTACTA CGATGTGCAG GCCCACTCCG CGGCCGAGGA GGAGAGTGTC 
CTGCGTCGGT TGCGCGAGGA AGACGGCGCC GTGGTGTCCG TGCGCATGGA GCGCTGCTAT 
CATCTGGAGT ACAGCGCTCA GGCAGAGCAC TCACTGGCCC TGGACGAGCT GCTGGTGTGG 
CTGGTCAAGC AACCGCTGAG CAAAGGCCAG AGCTTGTCCA GGCAACCTGC CCTGCAGTCA 
ACTGGGTCGA GTCAGTTGCT CCTGGAGATC GGGCCGCGTT TTAACTTCTC CACGCCGTAC 
TCCACGAACT GCGTGAACAT ATTCCAGAAT CTCGGGTACT CAGAGGTGCG TCGCATGGAA 
ACCTCCACCC GCTATCTGGT TACTTTTGGC GAGGGATCAA AGGCGCCGGA GGCAGCCAGG 
TTTGTTCCTC TGCTCGGTGA CCGCATGACC CAGTGCTTGT ACACCGAGGA GAATACCCCC 
AAGGCGAGCT TTGACGAGCA GCTACCTGAG CGCCAGGCCA ACTGGCATTT CGTGCCCGTT 
TTGGAGGAGG GTAGGGCGGC ACTGGAGCGG ATTAATCAGG AGCTGGGCTT AGCCTTCAAC 
GACTACGATT TGGACTACTA CCACGACTTG TTTGCCAAGG AGCTGGGCCG CAATCCCACC 
ACTGTGGAGC TCTTCGATTG CGCCCAGAGC AACAGTGAGC ACTCGCGCCA CTGGTTTTTC 
CGCGGACGTA TGGTGATCGA CGGCGTGGAG CAGCCCAAGT CGCTGATTCG CATGATCATG 
GACACGCAGG CCCACACGAA CCCGAACAAC ACCATTAAGT TCAGCGACAA CAGCAGTGCC 
ATGGTGGGAT TCGATCACCA GACCATAGTT CCGTCCTCCG TGGTCGCTCC CGGCGCAGTG 
CGTCTGCAGA GCGTGCAGTC TGACCTGATT TTCACGGCGG AGACCCACAA CATGCCCACT 
GCAGTGGCGC CTTTCAGCGG AGCCACCACC GGCACTGGCG GACGACTGCG TGATGTCCAG 
GGCGTGGGCA GAGGAGGCGT GCCGATCGCC GGCACCGCTG GCTACTGTGT TGGCGCTCTT 
CACATTCCAG GTTACAAACA GCCGTACGAG CCTTTGGACT TTAAATACCC TGCGACGTTT 
GCGCCCCCAC TTCAGGTGCT CATTGAGGCG AGCAATGGCG CCTCCGACTA CGGAAATAAG 
TTCGGCGAGC CAGTGATCTC TGGTTTTGCC CTCTCCTATG GACTGAACAG TGCTGCCGAT 
GCCAGCCAGC GGGATGAGTA CGTGAAACCG ATCATGTTCA GCGGTGGCTT GGGCACCATG 
CCCGCTACCA TGCGCGAGAA GCTGCCGCCG GCACGCGGTC AGTTGCTGGC CAAGATCGGG 
GGCCCAGTGT ACAGGATTGG AGTGGGTGGC GGCGCCGCAA GCTCTGTGGA GATACAAGGA 
TCCGGAGATG CTGAGCTAGA TTTTAATGCC GTACAGCGAG GAGACGCCGA AATGGAGAAT 
AAATTGAACC GTGTGGTGCG AGCCTGTCTA GACCTGGGCG AGCAAAACCC CATATTAGCC 
ATCCATGACC AGGGGGCTGG CGGCAACGGC AATGTCCTCA AGGAGCTAGT GGAACCAGGC 
TTTGCCGGAG CTGTTATCTT CTCCAAGGAG TTCCAACTCG GCGATCCCAC GATCACTGCC 
CTCGAACTGT GGGGTGCCGA GTACCAGGAA AACAATGCCA TTCTTTGCAA CGCTGATCAA 
CGCGAACTGC TGGAAAAGAT CTGCCGCCGT GAACGCTGTC CCATCAGCTT CGTGGGAGTG 
GTGACCGGAG ATGGGCGAGT AACGCTGCTG GAGAAGCCTG CCCCCAAGGA TCTGGAACAG 
GCGTTGAATG CGTCCAATCG CAGCGAAGTC TCACCGTTCG ACTTGGAACT TAAGTACGTT 
TTGGGCGACA TGCCCAAGAG AACGTACGAC TTGAAACGCG AACAGACGCC GCTCAAGGAG 
TTGAGTCTGC CGAAAGGTCT CCTTCTGGAC GAGGCCTTGG AGAGAGTTCT TAGCTTGGTG 
GCAGTCGGTA GCAAGCGCTT CCTGACCAAC AAGGTGGATC GCTGCGTCGG TGGATTGATT 
GCACAGCAAC AGTGCGTGGG ACCGCTCCAA GCTCCACTAG CGGACTACGC GTTAACCACC 
GTCTCCCACT TTAGTCACTC TGGAATCGCC ACTTCAATTG GTACACAGCC ACTAAAAGGA 
CTGCTTGATC CCGCCGCAAT GGCCAGGATG TGTGTGGCTG AAGCGTTGAG TAACCTTGTG 
TTTGTAAAGA TCAGCGAACT GGCTGATGTC AAGTGCTCTG GCAACTGGAT GTGGGCTGCA 
AAGCTGCCCG GCGAGGGTGC CCGGATGTTC GACGCCTGCA AGGAGCTCTG TCAGATTCTT 
GAGGAACTTC ACATCGCCAT CGATGGCGGA AAGGACTCCC TGTCCATGGC GGCTAAGGTG 
GGCGGTGAGA CAATAAAGTC CCCGGGTACT CTTGTCATCT CCACCTATGC TCCCTGTCCG 
GATGTGCGGC TAAAGGTAAC GCCTGATCTT AAAGGACCCG GCGCGGGGTC GAAGACTTCC 
CTGCTCTGGA TAAATCTGGA GAACAGCGCT CGCCTAGGTG GATCGGCTCT GGCTCAGGCA 
TACGCGCAGC AGGGCAAAGA TACGCCAAAC TTGACAAGGA GTGATGTGCT GGGCAAAGCA 
TTTGCTGTGA CTCAATCGTT GTTGGGAGAT GGACTTATTC AAGCAGGACA CGATGTCAGC 
GACGGAGGAT TGCTTGTTTG CGTATTGGAG ATGGCCATCG GAGGACTGAG TGGCCTTAGA 
GTGGATCTAT CGGAGCCCTT GGCCAAGCTC AAGAACTTCG ATAAGTCTGT CGAGAAACTT 
AATCGTCCCG AACTAGCTGT TCTCTTCGCC GAGGAGTGTG GATGGGTGGT GGAAGTCCTG 
GACACAGACC TGGAACGAGT TCGATCTACC TACGAAAAGG CAGGAGTACC AAACTACTAT 
TTGGGCGTGA CCGAGGGATT CGGACTGGAT TCCAGAGTGG TACTCAAGAA CGGCAAGTCA 
GAGCTGCTGG ACCAGCCACT CCGTGTGCTC TACAAGAAAT GGGAGAGAAC CAGTTACGAG 
CTGGAAAAGC TTCAGGCCAA CCCTGAGTGC GCCGAGGCAG AGTACAATAG CCTTGAGTAT 
CGCCAAGCAC CTCAGTATAG GGGACCTCAG AATGTGCAGG CCGAGCTTAC TCTCAAGCGA 
TCCTCCGCCC CAGTTCGCGT GGCCGTGCTC CGTGAGGAGG GTGTCAATAG TGAGCGCGAG 
ATGATGGCCT GCCTGCTGAG GGCTAACTTC GAGGTGCACG ATGTGACCAT GTCGGATCTT 
CTTCAAGGCA CCGCAAGCGT GTCCCAATAC CGCGGACTAA TTTTCCCGGG CGGCTTCAGC 
TATGCGGACA CTTTGGGATC CGCCAAGGGC TGGGCAGCAA ACATCCTCCA CAATCCCCGC 
TTGTTGCCTC AGTTTGAGGC TTTCAAGCGT CGTCAAGATG TGTTCTCCTT GGGCATTTGC 
AATGGTTGCC AGCTGATGAC ACTTATAGGA TTTGTTGGCA GTGCAAAGAG CGAGGTGGGC 
GCAGATCCCG ACGTGGCCCT GCTCCATAAC AAATCGCAGC GGTTCGAGTG TCGTTGGGCC 
ACCGTGAAGA TTCCAAGCAA TCGCTCCATC ATGCTCGGAA GTATGAAGGA TCTGGTTCTT 
GGCTGTTGGG TGGCTCACGG CGAAGGTCGC TTTGCATTCC GCGACGAGAA GCTCATTAGC 
CACTTGCAGT CGGAACAGCT GGTGACATTG CAATATGTTG ATGATGTAGG CAAACCGACG 
GAACTGTATC CACTTAACCC GAACGGTAGT CCTCAGGGAA TTGCTGGCCT TTGCTCGTCG 
GATGGACGTC ACTTGGCCCT CATGCCACAT CCCGAGCGAT GCTCCTCCAT GTACCAGTGG 
CCCTATGTGC CATCTTCATT CGAAGTTTCC CCCACACAAT CGGAAAGTCC TTGGCAGATT 
ATGTTTAACA ACGCGTACAA TTGGTGTGTC AAGTCGAATC AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pfas ( NM_164676.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723147.1 Drosophila melanogaster phosphoribosylformylglycinamidine synthase, transcript variant C (Pfas), mRNA.

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CloneID	OFa13865
Clone ID Related Accession (Same CDS sequence)	NM_057864.4 , NM_164675.2 , NM_164676.2
Accession Version	NM_164676.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	phosphoribosylformylglycinamidine synthase, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_164676.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTCATCC TTCGCTACTA CGATGTGCAG GCCCACTCCG CGGCCGAGGA GGAGAGTGTC 
CTGCGTCGGT TGCGCGAGGA AGACGGCGCC GTGGTGTCCG TGCGCATGGA GCGCTGCTAT 
CATCTGGAGT ACAGCGCTCA GGCAGAGCAC TCACTGGCCC TGGACGAGCT GCTGGTGTGG 
CTGGTCAAGC AACCGCTGAG CAAAGGCCAG AGCTTGTCCA GGCAACCTGC CCTGCAGTCA 
ACTGGGTCGA GTCAGTTGCT CCTGGAGATC GGGCCGCGTT TTAACTTCTC CACGCCGTAC 
TCCACGAACT GCGTGAACAT ATTCCAGAAT CTCGGGTACT CAGAGGTGCG TCGCATGGAA 
ACCTCCACCC GCTATCTGGT TACTTTTGGC GAGGGATCAA AGGCGCCGGA GGCAGCCAGG 
TTTGTTCCTC TGCTCGGTGA CCGCATGACC CAGTGCTTGT ACACCGAGGA GAATACCCCC 
AAGGCGAGCT TTGACGAGCA GCTACCTGAG CGCCAGGCCA ACTGGCATTT CGTGCCCGTT 
TTGGAGGAGG GTAGGGCGGC ACTGGAGCGG ATTAATCAGG AGCTGGGCTT AGCCTTCAAC 
GACTACGATT TGGACTACTA CCACGACTTG TTTGCCAAGG AGCTGGGCCG CAATCCCACC 
ACTGTGGAGC TCTTCGATTG CGCCCAGAGC AACAGTGAGC ACTCGCGCCA CTGGTTTTTC 
CGCGGACGTA TGGTGATCGA CGGCGTGGAG CAGCCCAAGT CGCTGATTCG CATGATCATG 
GACACGCAGG CCCACACGAA CCCGAACAAC ACCATTAAGT TCAGCGACAA CAGCAGTGCC 
ATGGTGGGAT TCGATCACCA GACCATAGTT CCGTCCTCCG TGGTCGCTCC CGGCGCAGTG 
CGTCTGCAGA GCGTGCAGTC TGACCTGATT TTCACGGCGG AGACCCACAA CATGCCCACT 
GCAGTGGCGC CTTTCAGCGG AGCCACCACC GGCACTGGCG GACGACTGCG TGATGTCCAG 
GGCGTGGGCA GAGGAGGCGT GCCGATCGCC GGCACCGCTG GCTACTGTGT TGGCGCTCTT 
CACATTCCAG GTTACAAACA GCCGTACGAG CCTTTGGACT TTAAATACCC TGCGACGTTT 
GCGCCCCCAC TTCAGGTGCT CATTGAGGCG AGCAATGGCG CCTCCGACTA CGGAAATAAG 
TTCGGCGAGC CAGTGATCTC TGGTTTTGCC CTCTCCTATG GACTGAACAG TGCTGCCGAT 
GCCAGCCAGC GGGATGAGTA CGTGAAACCG ATCATGTTCA GCGGTGGCTT GGGCACCATG 
CCCGCTACCA TGCGCGAGAA GCTGCCGCCG GCACGCGGTC AGTTGCTGGC CAAGATCGGG 
GGCCCAGTGT ACAGGATTGG AGTGGGTGGC GGCGCCGCAA GCTCTGTGGA GATACAAGGA 
TCCGGAGATG CTGAGCTAGA TTTTAATGCC GTACAGCGAG GAGACGCCGA AATGGAGAAT 
AAATTGAACC GTGTGGTGCG AGCCTGTCTA GACCTGGGCG AGCAAAACCC CATATTAGCC 
ATCCATGACC AGGGGGCTGG CGGCAACGGC AATGTCCTCA AGGAGCTAGT GGAACCAGGC 
TTTGCCGGAG CTGTTATCTT CTCCAAGGAG TTCCAACTCG GCGATCCCAC GATCACTGCC 
CTCGAACTGT GGGGTGCCGA GTACCAGGAA AACAATGCCA TTCTTTGCAA CGCTGATCAA 
CGCGAACTGC TGGAAAAGAT CTGCCGCCGT GAACGCTGTC CCATCAGCTT CGTGGGAGTG 
GTGACCGGAG ATGGGCGAGT AACGCTGCTG GAGAAGCCTG CCCCCAAGGA TCTGGAACAG 
GCGTTGAATG CGTCCAATCG CAGCGAAGTC TCACCGTTCG ACTTGGAACT TAAGTACGTT 
TTGGGCGACA TGCCCAAGAG AACGTACGAC TTGAAACGCG AACAGACGCC GCTCAAGGAG 
TTGAGTCTGC CGAAAGGTCT CCTTCTGGAC GAGGCCTTGG AGAGAGTTCT TAGCTTGGTG 
GCAGTCGGTA GCAAGCGCTT CCTGACCAAC AAGGTGGATC GCTGCGTCGG TGGATTGATT 
GCACAGCAAC AGTGCGTGGG ACCGCTCCAA GCTCCACTAG CGGACTACGC GTTAACCACC 
GTCTCCCACT TTAGTCACTC TGGAATCGCC ACTTCAATTG GTACACAGCC ACTAAAAGGA 
CTGCTTGATC CCGCCGCAAT GGCCAGGATG TGTGTGGCTG AAGCGTTGAG TAACCTTGTG 
TTTGTAAAGA TCAGCGAACT GGCTGATGTC AAGTGCTCTG GCAACTGGAT GTGGGCTGCA 
AAGCTGCCCG GCGAGGGTGC CCGGATGTTC GACGCCTGCA AGGAGCTCTG TCAGATTCTT 
GAGGAACTTC ACATCGCCAT CGATGGCGGA AAGGACTCCC TGTCCATGGC GGCTAAGGTG 
GGCGGTGAGA CAATAAAGTC CCCGGGTACT CTTGTCATCT CCACCTATGC TCCCTGTCCG 
GATGTGCGGC TAAAGGTAAC GCCTGATCTT AAAGGACCCG GCGCGGGGTC GAAGACTTCC 
CTGCTCTGGA TAAATCTGGA GAACAGCGCT CGCCTAGGTG GATCGGCTCT GGCTCAGGCA 
TACGCGCAGC AGGGCAAAGA TACGCCAAAC TTGACAAGGA GTGATGTGCT GGGCAAAGCA 
TTTGCTGTGA CTCAATCGTT GTTGGGAGAT GGACTTATTC AAGCAGGACA CGATGTCAGC 
GACGGAGGAT TGCTTGTTTG CGTATTGGAG ATGGCCATCG GAGGACTGAG TGGCCTTAGA 
GTGGATCTAT CGGAGCCCTT GGCCAAGCTC AAGAACTTCG ATAAGTCTGT CGAGAAACTT 
AATCGTCCCG AACTAGCTGT TCTCTTCGCC GAGGAGTGTG GATGGGTGGT GGAAGTCCTG 
GACACAGACC TGGAACGAGT TCGATCTACC TACGAAAAGG CAGGAGTACC AAACTACTAT 
TTGGGCGTGA CCGAGGGATT CGGACTGGAT TCCAGAGTGG TACTCAAGAA CGGCAAGTCA 
GAGCTGCTGG ACCAGCCACT CCGTGTGCTC TACAAGAAAT GGGAGAGAAC CAGTTACGAG 
CTGGAAAAGC TTCAGGCCAA CCCTGAGTGC GCCGAGGCAG AGTACAATAG CCTTGAGTAT 
CGCCAAGCAC CTCAGTATAG GGGACCTCAG AATGTGCAGG CCGAGCTTAC TCTCAAGCGA 
TCCTCCGCCC CAGTTCGCGT GGCCGTGCTC CGTGAGGAGG GTGTCAATAG TGAGCGCGAG 
ATGATGGCCT GCCTGCTGAG GGCTAACTTC GAGGTGCACG ATGTGACCAT GTCGGATCTT 
CTTCAAGGCA CCGCAAGCGT GTCCCAATAC CGCGGACTAA TTTTCCCGGG CGGCTTCAGC 
TATGCGGACA CTTTGGGATC CGCCAAGGGC TGGGCAGCAA ACATCCTCCA CAATCCCCGC 
TTGTTGCCTC AGTTTGAGGC TTTCAAGCGT CGTCAAGATG TGTTCTCCTT GGGCATTTGC 
AATGGTTGCC AGCTGATGAC ACTTATAGGA TTTGTTGGCA GTGCAAAGAG CGAGGTGGGC 
GCAGATCCCG ACGTGGCCCT GCTCCATAAC AAATCGCAGC GGTTCGAGTG TCGTTGGGCC 
ACCGTGAAGA TTCCAAGCAA TCGCTCCATC ATGCTCGGAA GTATGAAGGA TCTGGTTCTT 
GGCTGTTGGG TGGCTCACGG CGAAGGTCGC TTTGCATTCC GCGACGAGAA GCTCATTAGC 
CACTTGCAGT CGGAACAGCT GGTGACATTG CAATATGTTG ATGATGTAGG CAAACCGACG 
GAACTGTATC CACTTAACCC GAACGGTAGT CCTCAGGGAA TTGCTGGCCT TTGCTCGTCG 
GATGGACGTC ACTTGGCCCT CATGCCACAT CCCGAGCGAT GCTCCTCCAT GTACCAGTGG 
CCCTATGTGC CATCTTCATT CGAAGTTTCC CCCACACAAT CGGAAAGTCC TTGGCAGATT 
ATGTTTAACA ACGCGTACAA TTGGTGTGTC AAGTCGAATC AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723147.1
CDS	80..4144
Translation	MVILRYYDVQAHSAAEEESVLRRLREEDGAVVSVRMERCYHLEYSAQAEHSLALDELLVWLVKQPLSKGQSLSRQPALQSTGSSQLLLEIGPRFNFSTPYSTNCVNIFQNLGYSEVRRMETSTRYLVTFGEGSKAPEAARFVPLLGDRMTQCLYTEENTPKASFDEQLPERQANWHFVPVLEEGRAALERINQELGLAFNDYDLDYYHDLFAKELGRNPTTVELFDCAQSNSEHSRHWFFRGRMVIDGVEQPKSLIRMIMDTQAHTNPNNTIKFSDNSSAMVGFDHQTIVPSSVVAPGAVRLQSVQSDLIFTAETHNMPTAVAPFSGATTGTGGRLRDVQGVGRGGVPIAGTAGYCVGALHIPGYKQPYEPLDFKYPATFAPPLQVLIEASNGASDYGNKFGEPVISGFALSYGLNSAADASQRDEYVKPIMFSGGLGTMPATMREKLPPARGQLLAKIGGPVYRIGVGGGAASSVEIQGSGDAELDFNAVQRGDAEMENKLNRVVRACLDLGEQNPILAIHDQGAGGNGNVLKELVEPGFAGAVIFSKEFQLGDPTITALELWGAEYQENNAILCNADQRELLEKICRRERCPISFVGVVTGDGRVTLLEKPAPKDLEQALNASNRSEVSPFDLELKYVLGDMPKRTYDLKREQTPLKELSLPKGLLLDEALERVLSLVAVGSKRFLTNKVDRCVGGLIAQQQCVGPLQAPLADYALTTVSHFSHSGIATSIGTQPLKGLLDPAAMARMCVAEALSNLVFVKISELADVKCSGNWMWAAKLPGEGARMFDACKELCQILEELHIAIDGGKDSLSMAAKVGGETIKSPGTLVISTYAPCPDVRLKVTPDLKGPGAGSKTSLLWINLENSARLGGSALAQAYAQQGKDTPNLTRSDVLGKAFAVTQSLLGDGLIQAGHDVSDGGLLVCVLEMAIGGLSGLRVDLSEPLAKLKNFDKSVEKLNRPELAVLFAEECGWVVEVLDTDLERVRSTYEKAGVPNYYLGVTEGFGLDSRVVLKNGKSELLDQPLRVLYKKWERTSYELEKLQANPECAEAEYNSLEYRQAPQYRGPQNVQAELTLKRSSAPVRVAVLREEGVNSEREMMACLLRANFEVHDVTMSDLLQGTASVSQYRGLIFPGGFSYADTLGSAKGWAANILHNPRLLPQFEAFKRRQDVFSLGICNGCQLMTLIGFVGSAKSEVGADPDVALLHNKSQRFECRWATVKIPSNRSIMLGSMKDLVLGCWVAHGEGRFAFRDEKLISHLQSEQLVTLQYVDDVGKPTELYPLNPNGSPQGIAGLCSSDGRHLALMPHPERCSSMYQWPYVPSSFEVSPTQSESPWQIMFNNAYNWCVKSNQ

Target ORF information:

RefSeq Version	NM_164676.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster phosphoribosylformylglycinamidine synthase, transcript variant C (Pfas), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164676.2

ORF Insert Sequence:

ATGGTCATCC TTCGCTACTA CGATGTGCAG GCCCACTCCG CGGCCGAGGA GGAGAGTGTC 
CTGCGTCGGT TGCGCGAGGA AGACGGCGCC GTGGTGTCCG TGCGCATGGA GCGCTGCTAT 
CATCTGGAGT ACAGCGCTCA GGCAGAGCAC TCACTGGCCC TGGACGAGCT GCTGGTGTGG 
CTGGTCAAGC AACCGCTGAG CAAAGGCCAG AGCTTGTCCA GGCAACCTGC CCTGCAGTCA 
ACTGGGTCGA GTCAGTTGCT CCTGGAGATC GGGCCGCGTT TTAACTTCTC CACGCCGTAC 
TCCACGAACT GCGTGAACAT ATTCCAGAAT CTCGGGTACT CAGAGGTGCG TCGCATGGAA 
ACCTCCACCC GCTATCTGGT TACTTTTGGC GAGGGATCAA AGGCGCCGGA GGCAGCCAGG 
TTTGTTCCTC TGCTCGGTGA CCGCATGACC CAGTGCTTGT ACACCGAGGA GAATACCCCC 
AAGGCGAGCT TTGACGAGCA GCTACCTGAG CGCCAGGCCA ACTGGCATTT CGTGCCCGTT 
TTGGAGGAGG GTAGGGCGGC ACTGGAGCGG ATTAATCAGG AGCTGGGCTT AGCCTTCAAC 
GACTACGATT TGGACTACTA CCACGACTTG TTTGCCAAGG AGCTGGGCCG CAATCCCACC 
ACTGTGGAGC TCTTCGATTG CGCCCAGAGC AACAGTGAGC ACTCGCGCCA CTGGTTTTTC 
CGCGGACGTA TGGTGATCGA CGGCGTGGAG CAGCCCAAGT CGCTGATTCG CATGATCATG 
GACACGCAGG CCCACACGAA CCCGAACAAC ACCATTAAGT TCAGCGACAA CAGCAGTGCC 
ATGGTGGGAT TCGATCACCA GACCATAGTT CCGTCCTCCG TGGTCGCTCC CGGCGCAGTG 
CGTCTGCAGA GCGTGCAGTC TGACCTGATT TTCACGGCGG AGACCCACAA CATGCCCACT 
GCAGTGGCGC CTTTCAGCGG AGCCACCACC GGCACTGGCG GACGACTGCG TGATGTCCAG 
GGCGTGGGCA GAGGAGGCGT GCCGATCGCC GGCACCGCTG GCTACTGTGT TGGCGCTCTT 
CACATTCCAG GTTACAAACA GCCGTACGAG CCTTTGGACT TTAAATACCC TGCGACGTTT 
GCGCCCCCAC TTCAGGTGCT CATTGAGGCG AGCAATGGCG CCTCCGACTA CGGAAATAAG 
TTCGGCGAGC CAGTGATCTC TGGTTTTGCC CTCTCCTATG GACTGAACAG TGCTGCCGAT 
GCCAGCCAGC GGGATGAGTA CGTGAAACCG ATCATGTTCA GCGGTGGCTT GGGCACCATG 
CCCGCTACCA TGCGCGAGAA GCTGCCGCCG GCACGCGGTC AGTTGCTGGC CAAGATCGGG 
GGCCCAGTGT ACAGGATTGG AGTGGGTGGC GGCGCCGCAA GCTCTGTGGA GATACAAGGA 
TCCGGAGATG CTGAGCTAGA TTTTAATGCC GTACAGCGAG GAGACGCCGA AATGGAGAAT 
AAATTGAACC GTGTGGTGCG AGCCTGTCTA GACCTGGGCG AGCAAAACCC CATATTAGCC 
ATCCATGACC AGGGGGCTGG CGGCAACGGC AATGTCCTCA AGGAGCTAGT GGAACCAGGC 
TTTGCCGGAG CTGTTATCTT CTCCAAGGAG TTCCAACTCG GCGATCCCAC GATCACTGCC 
CTCGAACTGT GGGGTGCCGA GTACCAGGAA AACAATGCCA TTCTTTGCAA CGCTGATCAA 
CGCGAACTGC TGGAAAAGAT CTGCCGCCGT GAACGCTGTC CCATCAGCTT CGTGGGAGTG 
GTGACCGGAG ATGGGCGAGT AACGCTGCTG GAGAAGCCTG CCCCCAAGGA TCTGGAACAG 
GCGTTGAATG CGTCCAATCG CAGCGAAGTC TCACCGTTCG ACTTGGAACT TAAGTACGTT 
TTGGGCGACA TGCCCAAGAG AACGTACGAC TTGAAACGCG AACAGACGCC GCTCAAGGAG 
TTGAGTCTGC CGAAAGGTCT CCTTCTGGAC GAGGCCTTGG AGAGAGTTCT TAGCTTGGTG 
GCAGTCGGTA GCAAGCGCTT CCTGACCAAC AAGGTGGATC GCTGCGTCGG TGGATTGATT 
GCACAGCAAC AGTGCGTGGG ACCGCTCCAA GCTCCACTAG CGGACTACGC GTTAACCACC 
GTCTCCCACT TTAGTCACTC TGGAATCGCC ACTTCAATTG GTACACAGCC ACTAAAAGGA 
CTGCTTGATC CCGCCGCAAT GGCCAGGATG TGTGTGGCTG AAGCGTTGAG TAACCTTGTG 
TTTGTAAAGA TCAGCGAACT GGCTGATGTC AAGTGCTCTG GCAACTGGAT GTGGGCTGCA 
AAGCTGCCCG GCGAGGGTGC CCGGATGTTC GACGCCTGCA AGGAGCTCTG TCAGATTCTT 
GAGGAACTTC ACATCGCCAT CGATGGCGGA AAGGACTCCC TGTCCATGGC GGCTAAGGTG 
GGCGGTGAGA CAATAAAGTC CCCGGGTACT CTTGTCATCT CCACCTATGC TCCCTGTCCG 
GATGTGCGGC TAAAGGTAAC GCCTGATCTT AAAGGACCCG GCGCGGGGTC GAAGACTTCC 
CTGCTCTGGA TAAATCTGGA GAACAGCGCT CGCCTAGGTG GATCGGCTCT GGCTCAGGCA 
TACGCGCAGC AGGGCAAAGA TACGCCAAAC TTGACAAGGA GTGATGTGCT GGGCAAAGCA 
TTTGCTGTGA CTCAATCGTT GTTGGGAGAT GGACTTATTC AAGCAGGACA CGATGTCAGC 
GACGGAGGAT TGCTTGTTTG CGTATTGGAG ATGGCCATCG GAGGACTGAG TGGCCTTAGA 
GTGGATCTAT CGGAGCCCTT GGCCAAGCTC AAGAACTTCG ATAAGTCTGT CGAGAAACTT 
AATCGTCCCG AACTAGCTGT TCTCTTCGCC GAGGAGTGTG GATGGGTGGT GGAAGTCCTG 
GACACAGACC TGGAACGAGT TCGATCTACC TACGAAAAGG CAGGAGTACC AAACTACTAT 
TTGGGCGTGA CCGAGGGATT CGGACTGGAT TCCAGAGTGG TACTCAAGAA CGGCAAGTCA 
GAGCTGCTGG ACCAGCCACT CCGTGTGCTC TACAAGAAAT GGGAGAGAAC CAGTTACGAG 
CTGGAAAAGC TTCAGGCCAA CCCTGAGTGC GCCGAGGCAG AGTACAATAG CCTTGAGTAT 
CGCCAAGCAC CTCAGTATAG GGGACCTCAG AATGTGCAGG CCGAGCTTAC TCTCAAGCGA 
TCCTCCGCCC CAGTTCGCGT GGCCGTGCTC CGTGAGGAGG GTGTCAATAG TGAGCGCGAG 
ATGATGGCCT GCCTGCTGAG GGCTAACTTC GAGGTGCACG ATGTGACCAT GTCGGATCTT 
CTTCAAGGCA CCGCAAGCGT GTCCCAATAC CGCGGACTAA TTTTCCCGGG CGGCTTCAGC 
TATGCGGACA CTTTGGGATC CGCCAAGGGC TGGGCAGCAA ACATCCTCCA CAATCCCCGC 
TTGTTGCCTC AGTTTGAGGC TTTCAAGCGT CGTCAAGATG TGTTCTCCTT GGGCATTTGC 
AATGGTTGCC AGCTGATGAC ACTTATAGGA TTTGTTGGCA GTGCAAAGAG CGAGGTGGGC 
GCAGATCCCG ACGTGGCCCT GCTCCATAAC AAATCGCAGC GGTTCGAGTG TCGTTGGGCC 
ACCGTGAAGA TTCCAAGCAA TCGCTCCATC ATGCTCGGAA GTATGAAGGA TCTGGTTCTT 
GGCTGTTGGG TGGCTCACGG CGAAGGTCGC TTTGCATTCC GCGACGAGAA GCTCATTAGC 
CACTTGCAGT CGGAACAGCT GGTGACATTG CAATATGTTG ATGATGTAGG CAAACCGACG 
GAACTGTATC CACTTAACCC GAACGGTAGT CCTCAGGGAA TTGCTGGCCT TTGCTCGTCG 
GATGGACGTC ACTTGGCCCT CATGCCACAT CCCGAGCGAT GCTCCTCCAT GTACCAGTGG 
CCCTATGTGC CATCTTCATT CGAAGTTTCC CCCACACAAT CGGAAAGTCC TTGGCAGATT 
ATGTTTAACA ACGCGTACAA TTGGTGTGTC AAGTCGAATC AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Pfas cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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