TCOF1 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_005209634.4	XP_005209691.1	treacle protein isoform X3
XM_002689293.5	XP_002689339.2	treacle protein isoform X1
XM_010807517.2	XP_010805819.1	treacle protein isoform X1
XM_005209634.3	XP_005209691.1	treacle protein isoform X3
XM_010807519.2	XP_010805821.1	treacle protein isoform X4
XM_005199092.3	XP_005199149.1	treacle protein isoform X3
XM_010798512.2	XP_010796814.1	treacle protein isoform X4
XM_015472323.1	XP_015327809.1	treacle protein isoform X5
XM_010807517.3	XP_010805819.1	treacle protein isoform X1
XM_024995260.1	XP_024851028.1	treacle protein isoform X4
XM_591291.9	XP_591291.5	treacle protein isoform X1
XM_015464334.1	XP_015319820.1	treacle protein isoform X5
XM_010807518.2	XP_010805820.1	treacle protein isoform X2
XM_010798511.2	XP_010796813.1	treacle protein isoform X2
XM_002689293.6	XP_002689339.2	treacle protein isoform X1
XM_024995259.1	XP_024851027.1	treacle protein isoform X2
XM_010798510.2	XP_010796812.1	treacle protein isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa236767	XM_024995260.1 Latest version!	Bos taurus treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa236764	XM_010807517.3 Latest version!	Bos taurus treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa236764	XM_002689293.6 Latest version!	Bos taurus treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa236765	XM_024995259.1 Latest version!	Bos taurus treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa236766	XM_005209634.4 Latest version!	Bos taurus treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa05568	XM_010807517.2 Latest version!	Bos taurus Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa05568	XM_591291.9 Latest version!	Bos taurus Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa05568	XM_010798510.2 Latest version!	Bos taurus Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa05568	XM_002689293.5 Latest version!	Bos taurus Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa05570	XM_005209634.3 Latest version!	Bos taurus Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa05570	XM_005199092.3 Latest version!	Bos taurus Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa48671	XM_010807518.2 Latest version!	Bos taurus Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa48671	XM_010798511.2 Latest version!	Bos taurus Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa48672	XM_010807519.2 Latest version!	Bos taurus Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa48672	XM_010798512.2 Latest version!	Bos taurus Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa90352	XM_015472323.1 Latest version!	Bos taurus Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa90352	XM_015464334.1 Latest version!	Bos taurus Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00

TCOF1 ( XM_024995260.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024851028.1 Bos taurus treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OBa236767
Clone ID Related Accession (Same CDS sequence)	XM_024995260.1
Accession Version	XM_024995260.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2880bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	treacle protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAGG CCAGGAAGCG GCGAGAGCTC CTTCCCCTGA TCTACCAGCA TCTGCTGCAG 
GCGGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA AATTTTCCTG 
AGTCAGCCTG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACTTC GGAGATTGGC 
CGGAAACGGA AGGCAGAGGA AGATGCGGCC TTGCAGGCCA AGAGGAGCCG TGTGTCAGAC 
CCCATCAGCA GCTCAGAAAG CTCAGAAGAG GAGGAGGAAG AGGAGGAGGC AGAAGCCAAA 
ACCACCAAAC CCACCCCGAG ATTAGCAGCT ACCAACTCCT CAGTCACAGG GACAGTTTCG 
TCTTCAAATG TGAAAGAAAA AGCCAAGGCA AAGACCACGA AAGCCAGCAA GACGGTGAAC 
TCCACGACAC ACCCTGCCCC GGGAAAGGCA GTGGCCCACC TCCTCACTGG GAAGTCACCC 
CAGAAGACAT CGGGGCCCTC GGCAAATGCT GTCTTGGTGT CAGAAACCGA GGAGGAGGGC 
AGCGTGTCAG CCCTCAGAAC CGCCACCACG CCTGGAATGG CATCCGCCGA CCAGGCTGAC 
AGCTCCAGTG AGGATACCAC CAGCTCGAGT GATGAGACGG ACTTGGAGGC GAAAGCCGCA 
GTAAAACCAC TGCAGGTCAG AGCCTCAGCC ACTGCAGTCA AGGAGTCTCC GAGAAAAAGT 
GCAGCCCCGA CTCCAGGGAA GGCAGGGGTT GTGACACCCC AAGTCAGAGG CGGTGCTGTG 
ACCCCAGCCA GCAGGGCCAA GAAGCCAGAA GAGGTCTCAG AAAGCAGTGA AGAGTCCGAC 
GAGAGTGAGG AGGCCGCCCC TGCGGGGACA CCCAGTCAGG TGAAGGCCTT GGAGAAAACC 
GTCCCAATCA GAGTTGCTCC TAGTCCTGCC AAGGGGACCC CTGGGAAAGG GGCCACCCCA 
GCACCCCCTG GGAAGGCAGG GCCCTTAGGG AAGCCAGAGG AGGACTCGGA GAGCAGCAGC 
GAGGAGTCAG ACAGCGAGGA GGAGACACAA GCTGTCAAGA CCCCAGTGCA GGCAAAGCCC 
TCTGGGAAGA TCCCCCAGGT TAAAGCTGCC TCCACACCAA AAATCCAGGT GTTTTTCCCT 
AAGAAAGGGT CCCCTCCAAC ATCCCCTGGC AAGGCAGGGC CTGCAGCCAC CCAAGCCCAG 
CCCAGGAAGC AGGAGGAGGA GGAGAGCAGC AGCAGCAGCA GCAAGGAATC AGACAGCGAG 
GGCGAGGTGC CCACGGCCAT GCAGCAGACT ACAAGCCTGG CTCAGGCAAA GCCCTTGGAG 
AAAAACTCTC AGGTCAGAGC TGCCTCAGCC ACAGGTGCAG GACCCTCGGC AAAGGGCACG 
GTCCCAGCGC TCCCTCAGAA GGCAAGGTCT GTGGCCGCCC AGGATGGGAA GCAGGAGGAC 
TCAGAGAGCA GCAGTGAGGA GGAGTCAGAC AGCGAGGAGG AGATGCAGAA GGCAGGGACC 
TCAGCCCAGG CACAGTCCTC TGGGAAAGCC CTCCAGATCA GACCTGCCTC CGGTCCCACC 
AAGAGGCCCC CTCAGAAGGC CGGGCCTGCA ACCACCCAGG TCAAGGTTGA AAGGGCCGAG 
GAAGACTCGG AGAGCAGTGA GGAGGAATCA GACAGTGAGG ATGAAGCTCC TGTAGCCAAG 
ACTCCAGCCC AGGCAAAATC AGCTGTGAAA ACTTCTCAGA TCAAGGCCTC CCCAAGGAAG 
GGCACCCCCA TTACACCCTC ATCTGCCAGG GTCCCCCCAG TGCAAGTCGG CACACCAGCC 
CCCCGGACAG CAGCAGCAGT GAGTACGCCC ACCTGCGCGT CATCCCCAGC CATAGTCAGG 
CACACCCAGA AGCCAGGGGA GGACTCTTCA AGCAGCGAGG AGTCCGAGAG CGAGGAGGAG 
ACAGCTCCTG CTGCAGCAGG GGGACAGATG AAGTCTATAG GGAAGACCCT CCCAGTGAAA 
GCTGCCTCAC CAGCCACCAA GGGGCCCTCA GGAAAAGGGA CCACCCCAGG GCCCCCTGGG 
AAGGCAGGAC CTGCAGTGGC ACAGGTCAAG GCTGAGGTGC AGGAAGACTC AGAAAGCAGT 
GAGGAGTCGG ACAATGAGGA AGCGGCTGCA ACTCCCGCAC AGGTGAAGAC TTCAGTGAAA 
ACCCCTCAGA CCAAAGCCAA CTCTTCTGCC ACCAGAGTGG CTTCAGCCAA AGCAGCAGCA 
CCAGCTCCTG GAAAGGGGGG CCTTGCTGTT GCTCAAGCCA AAGTGGGGTC CCCAGCCAAG 
GTAAAACCAC CAGCGAGAAC CCCCCAGAGT AGCGCCGTCT CTGGGAGGGG CCAGGTGTCT 
GTGCCGGCTG TGGGGAAGGC AGCAGCTGCA GCAGCCCAGG CCCAGCCGGG GCCTGTCAAG 
GGCTCACAGG AGGACTCGGA GAGCAGTGAG GAGGAGTCGG ACAGTGAGGG AGAGGCACCT 
CCTCAGGGGA AGCCCTCAGG AAAGACCCCC CAGGTCAGAA CTGCCTCAGC CCCCACCAAG 
GCGTCCCCCA AGAAAGGGGC TGCCCCAGTA CCCCCTGGGA AAGCAGGACC CCCAGCCACC 
CAGGCCAAGA GGCAGGAGGA GGACTCGGAG AGCAGCAGCG AGGAGGAGTC GGACAGTGAC 
GGGGACGTGC CAGCTGCTGC ACCTCCAGCC CAGAAAGAGG GTAGCTCCAA AACTGCCACA 
AGCAAGACCC TGGCCCCAGC GTCCCTGGAG AAGAAAGCAG AGGAGTCCTC AACCAGCAGT 
GATGAGGACC TGCCATCCAG CCAGTCCAGC TGGTCCAGCT GCTACCCCCG CCCAAGCGCA 
GGCTACAGGC ACCCTGAGGA AGGCTCAGGC CTTGGAGAGC ACAGCCAGGA GCTCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024851028.1
CDS	61..2940
Translation	MAEARKRRELLPLIYQHLLQAGYVRAAREVKEQSGQKIFLSQPVTLLDIYTHWQQTSEIGRKRKAEEDAALQAKRSRVSDPISSSESSEEEEEEEEAEAKTTKPTPRLAATNSSVTGTVSSSNVKEKAKAKTTKASKTVNSTTHPAPGKAVAHLLTGKSPQKTSGPSANAVLVSETEEEGSVSALRTATTPGMASADQADSSSEDTTSSSDETDLEAKAAVKPLQVRASATAVKESPRKSAAPTPGKAGVVTPQVRGGAVTPASRAKKPEEVSESSEESDESEEAAPAGTPSQVKALEKTVPIRVAPSPAKGTPGKGATPAPPGKAGPLGKPEEDSESSSEESDSEEETQAVKTPVQAKPSGKIPQVKAASTPKIQVFFPKKGSPPTSPGKAGPAATQAQPRKQEEEESSSSSSKESDSEGEVPTAMQQTTSLAQAKPLEKNSQVRAASATGAGPSAKGTVPALPQKARSVAAQDGKQEDSESSSEEESDSEEEMQKAGTSAQAQSSGKALQIRPASGPTKRPPQKAGPATTQVKVERAEEDSESSEEESDSEDEAPVAKTPAQAKSAVKTSQIKASPRKGTPITPSSARVPPVQVGTPAPRTAAAVSTPTCASSPAIVRHTQKPGEDSSSSEESESEEETAPAAAGGQMKSIGKTLPVKAASPATKGPSGKGTTPGPPGKAGPAVAQVKAEVQEDSESSEESDNEEAAATPAQVKTSVKTPQTKANSSATRVASAKAAAPAPGKGGLAVAQAKVGSPAKVKPPARTPQSSAVSGRGQVSVPAVGKAAAAAAQAQPGPVKGSQEDSESSEEESDSEGEAPPQGKPSGKTPQVRTASAPTKASPKKGAAPVPPGKAGPPATQAKRQEEDSESSSEEESDSDGDVPAAAPPAQKEGSSKTATSKTLAPASLEKKAEESSTSSDEDLPSSQSSWSSCYPRPSAGYRHPEEGSGLGEHSQELL

Target ORF information:

RefSeq Version	XM_024995260.1
Organism	Bos taurus(cattle)
Definition	Bos taurus treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024995260.1

ORF Insert Sequence:

ATGGCTGAGG CCAGGAAGCG GCGAGAGCTC CTTCCCCTGA TCTACCAGCA TCTGCTGCAG 
GCGGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA AATTTTCCTG 
AGTCAGCCTG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACTTC GGAGATTGGC 
CGGAAACGGA AGGCAGAGGA AGATGCGGCC TTGCAGGCCA AGAGGAGCCG TGTGTCAGAC 
CCCATCAGCA GCTCAGAAAG CTCAGAAGAG GAGGAGGAAG AGGAGGAGGC AGAAGCCAAA 
ACCACCAAAC CCACCCCGAG ATTAGCAGCT ACCAACTCCT CAGTCACAGG GACAGTTTCG 
TCTTCAAATG TGAAAGAAAA AGCCAAGGCA AAGACCACGA AAGCCAGCAA GACGGTGAAC 
TCCACGACAC ACCCTGCCCC GGGAAAGGCA GTGGCCCACC TCCTCACTGG GAAGTCACCC 
CAGAAGACAT CGGGGCCCTC GGCAAATGCT GTCTTGGTGT CAGAAACCGA GGAGGAGGGC 
AGCGTGTCAG CCCTCAGAAC CGCCACCACG CCTGGAATGG CATCCGCCGA CCAGGCTGAC 
AGCTCCAGTG AGGATACCAC CAGCTCGAGT GATGAGACGG ACTTGGAGGC GAAAGCCGCA 
GTAAAACCAC TGCAGGTCAG AGCCTCAGCC ACTGCAGTCA AGGAGTCTCC GAGAAAAAGT 
GCAGCCCCGA CTCCAGGGAA GGCAGGGGTT GTGACACCCC AAGTCAGAGG CGGTGCTGTG 
ACCCCAGCCA GCAGGGCCAA GAAGCCAGAA GAGGTCTCAG AAAGCAGTGA AGAGTCCGAC 
GAGAGTGAGG AGGCCGCCCC TGCGGGGACA CCCAGTCAGG TGAAGGCCTT GGAGAAAACC 
GTCCCAATCA GAGTTGCTCC TAGTCCTGCC AAGGGGACCC CTGGGAAAGG GGCCACCCCA 
GCACCCCCTG GGAAGGCAGG GCCCTTAGGG AAGCCAGAGG AGGACTCGGA GAGCAGCAGC 
GAGGAGTCAG ACAGCGAGGA GGAGACACAA GCTGTCAAGA CCCCAGTGCA GGCAAAGCCC 
TCTGGGAAGA TCCCCCAGGT TAAAGCTGCC TCCACACCAA AAATCCAGGT GTTTTTCCCT 
AAGAAAGGGT CCCCTCCAAC ATCCCCTGGC AAGGCAGGGC CTGCAGCCAC CCAAGCCCAG 
CCCAGGAAGC AGGAGGAGGA GGAGAGCAGC AGCAGCAGCA GCAAGGAATC AGACAGCGAG 
GGCGAGGTGC CCACGGCCAT GCAGCAGACT ACAAGCCTGG CTCAGGCAAA GCCCTTGGAG 
AAAAACTCTC AGGTCAGAGC TGCCTCAGCC ACAGGTGCAG GACCCTCGGC AAAGGGCACG 
GTCCCAGCGC TCCCTCAGAA GGCAAGGTCT GTGGCCGCCC AGGATGGGAA GCAGGAGGAC 
TCAGAGAGCA GCAGTGAGGA GGAGTCAGAC AGCGAGGAGG AGATGCAGAA GGCAGGGACC 
TCAGCCCAGG CACAGTCCTC TGGGAAAGCC CTCCAGATCA GACCTGCCTC CGGTCCCACC 
AAGAGGCCCC CTCAGAAGGC CGGGCCTGCA ACCACCCAGG TCAAGGTTGA AAGGGCCGAG 
GAAGACTCGG AGAGCAGTGA GGAGGAATCA GACAGTGAGG ATGAAGCTCC TGTAGCCAAG 
ACTCCAGCCC AGGCAAAATC AGCTGTGAAA ACTTCTCAGA TCAAGGCCTC CCCAAGGAAG 
GGCACCCCCA TTACACCCTC ATCTGCCAGG GTCCCCCCAG TGCAAGTCGG CACACCAGCC 
CCCCGGACAG CAGCAGCAGT GAGTACGCCC ACCTGCGCGT CATCCCCAGC CATAGTCAGG 
CACACCCAGA AGCCAGGGGA GGACTCTTCA AGCAGCGAGG AGTCCGAGAG CGAGGAGGAG 
ACAGCTCCTG CTGCAGCAGG GGGACAGATG AAGTCTATAG GGAAGACCCT CCCAGTGAAA 
GCTGCCTCAC CAGCCACCAA GGGGCCCTCA GGAAAAGGGA CCACCCCAGG GCCCCCTGGG 
AAGGCAGGAC CTGCAGTGGC ACAGGTCAAG GCTGAGGTGC AGGAAGACTC AGAAAGCAGT 
GAGGAGTCGG ACAATGAGGA AGCGGCTGCA ACTCCCGCAC AGGTGAAGAC TTCAGTGAAA 
ACCCCTCAGA CCAAAGCCAA CTCTTCTGCC ACCAGAGTGG CTTCAGCCAA AGCAGCAGCA 
CCAGCTCCTG GAAAGGGGGG CCTTGCTGTT GCTCAAGCCA AAGTGGGGTC CCCAGCCAAG 
GTAAAACCAC CAGCGAGAAC CCCCCAGAGT AGCGCCGTCT CTGGGAGGGG CCAGGTGTCT 
GTGCCGGCTG TGGGGAAGGC AGCAGCTGCA GCAGCCCAGG CCCAGCCGGG GCCTGTCAAG 
GGCTCACAGG AGGACTCGGA GAGCAGTGAG GAGGAGTCGG ACAGTGAGGG AGAGGCACCT 
CCTCAGGGGA AGCCCTCAGG AAAGACCCCC CAGGTCAGAA CTGCCTCAGC CCCCACCAAG 
GCGTCCCCCA AGAAAGGGGC TGCCCCAGTA CCCCCTGGGA AAGCAGGACC CCCAGCCACC 
CAGGCCAAGA GGCAGGAGGA GGACTCGGAG AGCAGCAGCG AGGAGGAGTC GGACAGTGAC 
GGGGACGTGC CAGCTGCTGC ACCTCCAGCC CAGAAAGAGG GTAGCTCCAA AACTGCCACA 
AGCAAGACCC TGGCCCCAGC GTCCCTGGAG AAGAAAGCAG AGGAGTCCTC AACCAGCAGT 
GATGAGGACC TGCCATCCAG CCAGTCCAGC TGGTCCAGCT GCTACCCCCG CCCAAGCGCA 
GGCTACAGGC ACCCTGAGGA AGGCTCAGGC CTTGGAGAGC ACAGCCAGGA GCTCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_010807517.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010805819.1 Bos taurus treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa236764
Clone ID Related Accession (Same CDS sequence)	XM_010807517.3 , XM_002689293.6
Accession Version	XM_010807517.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4377bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	treacle protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010807517.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAGG CCAGGAAGCG GCGAGAGCTC CTTCCCCTGA TCTACCAGCA TCTGCTGCAG 
GCGGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA AATTTTCCTG 
AGTCAGCCTG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACTTC GGAGATTGGC 
CGGAAACGGA AGGCAGAGGA AGATGCGGCC TTGCAGGCCA AGAGGAGCCG TGTGTCAGAC 
CCCATCAGCA GCTCAGAAAG CTCAGAAGAG GAGGAGGAAG AGGAGGAGGC AGAAGCCAAA 
ACCACCAAAC CCACCCCGAG ATTAGCAGCT ACCAACTCCT CAGTCACAGG GACAGTTTCG 
TCTTCAAATG TGAAAGAAAA AGCCAAGGCA AAGACCACGA AAGCCAGCAA GACGGTGAAC 
TCCACGACAC ACCCTGCCCC GGGAAAGGCA GTGGCCCACC TCCTCACTGG GAAGTCACCC 
CAGAAGACAT CGGGGCCCTC GGCAAATGCT GTCTTGGTGT CAGAAACCGA GGAGGAGGGC 
AGCGTGTCAG CCCTCAGAAC CGCCACCACG CCTGGAATGG CATCCGCCGA CCAGGCTGAC 
AGCTCCAGTG AGGATACCAC CAGCTCGAGT GATGAGACGG ACTTGGAGGC GAAAGCCGCA 
GTAAAACCAC TGCAGGTCAG AGCCTCAGCC ACTGCAGTCA AGGAGTCTCC GAGAAAAAGT 
GCAGCCCCGA CTCCAGGGAA GGCAGGGGTT GTGACACCCC AAGTCAGAGG CGGTGCTGTG 
ACCCCAGCCA GCAGGGCCAA GAAGCCAGAA GAGGTCTCAG AAAGCAGTGA AGAGTCCGAC 
GAGAGTGAGG AGGCCGCCCC TGCGGGGACA CCCAGTCAGG TGAAGGCCTT GGAGAAAACC 
GTCCCAATCA GAGTTGCTCC TAGTCCTGCC AAGGGGACCC CTGGGAAAGG GGCCACCCCA 
GCACCCCCTG GGAAGGCAGG GCCCTTAGGG AAGCCAGAGG AGGACTCGGA GAGCAGCAGC 
GAGGAGTCAG ACAGCGAGGA GGAGACACAA GCTGTCAAGA CCCCAGTGCA GGCAAAGCCC 
TCTGGGAAGA TCCCCCAGGT TAAAGCTGCC TCCACACCAA AAATCCAGGT GTTTTTCCCT 
AAGAAAGGGT CCCCTCCAAC ATCCCCTGGC AAGGCAGGGC CTGCAGCCAC CCAAGCCCAG 
CCCAGGAAGC AGGAGGAGGA GGAGAGCAGC AGCAGCAGCA GCAAGGAATC AGACAGCGAG 
GGCGAGGTGC CCACGGCCAT GCAGCAGACT ACAAGCCTGG CTCAGGCAAA GCCCTTGGAG 
AAAAACTCTC AGGTCAGAGC TGCCTCAGCC ACAGGTGCAG GACCCTCGGC AAAGGGCACG 
GTCCCAGCGC TCCCTCAGAA GGCAAGGTCT GTGGCCGCCC AGGATGGGAA GCAGGAGGAC 
TCAGAGAGCA GCAGTGAGGA GGAGTCAGAC AGCGAGGAGG AGATGCAGAA GGCAGGGACC 
TCAGCCCAGG CACAGTCCTC TGGGAAAGCC CTCCAGATCA GACCTGCCTC CGGTCCCACC 
AAGAGGCCCC CTCAGAAGGC CGGGCCTGCA ACCACCCAGG TCAAGGTTGA AAGGGCCGAG 
GAAGACTCGG AGAGCAGTGA GGAGGAATCA GACAGTGAGG ATGAAGCTCC TGTAGCCAAG 
ACTCCAGCCC AGGCAAAATC AGCTGTGAAA ACTTCTCAGA TCAAGGCCTC CCCAAGGAAG 
GGCACCCCCA TTACACCCTC ATCTGCCAGG GTCCCCCCAG TGCAAGTCGG CACACCAGCC 
CCCCGGACAG CAGCAGCAGT GAGTACGCCC ACCTGCGCGT CATCCCCAGC CATAGTCAGG 
CACACCCAGA AGCCAGGGGA GGACTCTTCA AGCAGCGAGG AGTCCGAGAG CGAGGAGGAG 
ACAGCTCCTG CTGCAGCAGG GGGACAGATG AAGTCTATAG GGAAGACCCT CCCAGTGAAA 
GCTGCCTCAC CAGCCACCAA GGGGCCCTCA GGAAAAGGGA CCACCCCAGG GCCCCCTGGG 
AAGGCAGGAC CTGCAGTGGC ACAGGTCAAG GCTGAGGTGC AGGAAGACTC AGAAAGCAGT 
GAGGAGTCGG ACAATGAGGA AGCGGCTGCA ACTCCCGCAC AGGTGAAGAC TTCAGTGAAA 
ACCCCTCAGA CCAAAGCCAA CTCTTCTGCC ACCAGAGTGG CTTCAGCCAA AGCAGCAGCA 
CCAGCTCCTG GAAAGGGGGG CCTTGCTGTT GCTCAAGCCA AAGTGGGGTC CCCAGCCAAG 
GTAAAACCAC CAGCGAGAAC CCCCCAGAGT AGCGCCGTCT CTGGGAGGGG CCAGGTGTCT 
GTGCCGGCTG TGGGGAAGGC AGCAGCTGCA GCAGCCCAGG CCCAGCCGGG GCCTGTCAAG 
GGCTCACAGG AGGACTCGGA GAGCAGTGAG GAGGAGTCGG ACAGTGAGGG AGAGGCACCT 
CCTCAGGGGA AGCCCTCAGG AAAGACCCCC CAGGTCAGAA CTGCCTCAGC CCCCACCAAG 
GCGTCCCCCA AGAAAGGGGC TGCCCCAGTA CCCCCTGGGA AAGCAGGACC CCCAGCCACC 
CAGGCCAAGA GGCAGGAGGA GGACTCGGAG AGCAGCAGCG AGGAGGAGTC GGACAGTGAC 
GGGGACGTGC CAGCTGCTGC ACCTCCAGCC CAGAAAGAGG GTAGCTCCAA AACTGCCACA 
AGCAAGACCC TGGCCCCAGC GTCCCTGGAG AAGAAAGCAG AGGAGTCCTC AACCAGCAGT 
GATGAGGACC TGCCATCCAG CCAGGTGATT AAAACCCCTC TGATTTTTGT CGACCCTAAT 
CGTAGTCCAG CTGGTCCAGC TGCTACCCCC GCCCAAGCGC AGGCTACAGG CACCCTGAGG 
AAGGCTCAGG CCTTGGAGAG CACAGCCAGG AGCTCCTCTG AGAGCGAGGA CGAGGACGTG 
ATCCCCGCTA CACAGTGCCC GACTCCTGCC ACTAGGACCA GCGTGGTGAC TGTGCCCACG 
GCACCCCCAA AGGCGGCCCT CAGAGCCAGT GTGGTGGGGG CTCCCAGCAG TGAGGACACA 
AGTCAGGGGT CTGAAGGCAA GAAGCAGGAG GCCTCAGCCA CTCAGGTGAC CAAGAGGAAC 
CTCCCGCTGA CACAGGCTGC CCTGAAGGTT CTTGCCCAGA AAGCCAGTGA GGCCCAATCT 
CCTGCTGCCA AGACCCCGTC TTCAAGTGGG ACTGACAGTG CTGTGGGAAC ACTCTCCACG 
GCAAGTCCCC AGAGCACCCC TACCCAGGTC AGAATGGCCA ACAAGCTCAG AAAGCCTGAG 
GTTCCTACAG CTCAGCAGGC CACAGCCACT CCCTCGGGAC ACCCCACAGC CAAGGCCCCT 
GCAACCTCAG ATGACAGCAA CAGCAGCTCT TCAGGGAGCG AGGACGATGC CAAGGGGTCC 
CAGGCGGCCC CGTCAGCCCA CAGGCCAGGC CCAGCCCCCT CCAGGAGGGA GACCTTGGTG 
GAAGAGACCA CAACAGAGTC CAGTGACGAC GAGGTGGTGG CACCTTCCCA GTCTCTCCTC 
TCAGGTTTTG TGACTCCTGG GCTGACTCCA GCTGGTTCCA AGACTTCAAA GGCCGCTCCC 
AAACCAGACG CCAGCCCCTC TGTTTCCTCT ACTCCGGCCA CCAGAGATGC CCCAGAGGGC 
AAGCAGGAAG TGGAGCCCCA ACAAGCAGCA GGCACCATGT CCCCTAAAAC AGGCAGAAAA 
GAGGCTGGTG CCACACCTCA GAAGCCCGGG AAGGGGCTCG GGAGTCCCCC GGCCTCCATG 
CTGGCGCTGC AGAGCAACAT CACCCAGCGC CTCCTGAGCG AGCCCTGGCC CCTGAGCGAG 
GCGCAGGTGC AGGCCTCGGT GGCGAAGGTG CTCACGGAGC TGCTGGAGCA GGAGAGGAAG 
AAGGCCATGG ACACCGCCAA GGAAGGCAGC CGGAAGGGCC GCCTGGGCCA TAAGCGGAAG 
CTGTCCGAAG ACCAGACAGC CCCCAAGGCC CCCAAGAACA AGAAAAAGAA GCAGCTGGCA 
GCTGCAGATG GTGGGGAGCA TGTCGTCTCC TCAGAAAAGG CCCCCAGGAC TGTCAAAGGG 
AAATCCAAGA AGGACAGAGC AAGTGGTGAC GTCAAGGAGA AGAAGGAGAA GGAGTCTCCC 
GGCTCTCAAG GGGCCAAGGA GAAGCCAGTA GGGGAGCTGG GGACTCCAAA GGGTGACGGG 
GGAGACCACG GCAACCTTAA GATGAAGAAA GAGAAGAAGA AATCTGACAA GAAAAAAAAA 
GACAAGGAGA AAAAGGAAAA GAAGAAGAAA GCAAAAAAGG CCTCAACCAA AGACCCTGGC 
TCACCATCAC AGAAGAAAAA GAAGAGAAAG AAGAAGACAG CAGAGCAAAC CGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010805819.1
CDS	64..4440
Translation	MAEARKRRELLPLIYQHLLQAGYVRAAREVKEQSGQKIFLSQPVTLLDIYTHWQQTSEIGRKRKAEEDAALQAKRSRVSDPISSSESSEEEEEEEEAEAKTTKPTPRLAATNSSVTGTVSSSNVKEKAKAKTTKASKTVNSTTHPAPGKAVAHLLTGKSPQKTSGPSANAVLVSETEEEGSVSALRTATTPGMASADQADSSSEDTTSSSDETDLEAKAAVKPLQVRASATAVKESPRKSAAPTPGKAGVVTPQVRGGAVTPASRAKKPEEVSESSEESDESEEAAPAGTPSQVKALEKTVPIRVAPSPAKGTPGKGATPAPPGKAGPLGKPEEDSESSSEESDSEEETQAVKTPVQAKPSGKIPQVKAASTPKIQVFFPKKGSPPTSPGKAGPAATQAQPRKQEEEESSSSSSKESDSEGEVPTAMQQTTSLAQAKPLEKNSQVRAASATGAGPSAKGTVPALPQKARSVAAQDGKQEDSESSSEEESDSEEEMQKAGTSAQAQSSGKALQIRPASGPTKRPPQKAGPATTQVKVERAEEDSESSEEESDSEDEAPVAKTPAQAKSAVKTSQIKASPRKGTPITPSSARVPPVQVGTPAPRTAAAVSTPTCASSPAIVRHTQKPGEDSSSSEESESEEETAPAAAGGQMKSIGKTLPVKAASPATKGPSGKGTTPGPPGKAGPAVAQVKAEVQEDSESSEESDNEEAAATPAQVKTSVKTPQTKANSSATRVASAKAAAPAPGKGGLAVAQAKVGSPAKVKPPARTPQSSAVSGRGQVSVPAVGKAAAAAAQAQPGPVKGSQEDSESSEEESDSEGEAPPQGKPSGKTPQVRTASAPTKASPKKGAAPVPPGKAGPPATQAKRQEEDSESSSEEESDSDGDVPAAAPPAQKEGSSKTATSKTLAPASLEKKAEESSTSSDEDLPSSQVIKTPLIFVDPNRSPAGPAATPAQAQATGTLRKAQALESTARSSSESEDEDVIPATQCPTPATRTSVVTVPTAPPKAALRASVVGAPSSEDTSQGSEGKKQEASATQVTKRNLPLTQAALKVLAQKASEAQSPAAKTPSSSGTDSAVGTLSTASPQSTPTQVRMANKLRKPEVPTAQQATATPSGHPTAKAPATSDDSNSSSSGSEDDAKGSQAAPSAHRPGPAPSRRETLVEETTTESSDDEVVAPSQSLLSGFVTPGLTPAGSKTSKAAPKPDASPSVSSTPATRDAPEGKQEVEPQQAAGTMSPKTGRKEAGATPQKPGKGLGSPPASMLALQSNITQRLLSEPWPLSEAQVQASVAKVLTELLEQERKKAMDTAKEGSRKGRLGHKRKLSEDQTAPKAPKNKKKKQLAAADGGEHVVSSEKAPRTVKGKSKKDRASGDVKEKKEKESPGSQGAKEKPVGELGTPKGDGGDHGNLKMKKEKKKSDKKKKDKEKKEKKKKAKKASTKDPGSPSQKKKKRKKKTAEQTV

Target ORF information:

RefSeq Version	XM_010807517.3
Organism	Bos taurus(cattle)
Definition	Bos taurus treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010807517.3

ORF Insert Sequence:

ATGGCTGAGG CCAGGAAGCG GCGAGAGCTC CTTCCCCTGA TCTACCAGCA TCTGCTGCAG 
GCGGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA AATTTTCCTG 
AGTCAGCCTG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACTTC GGAGATTGGC 
CGGAAACGGA AGGCAGAGGA AGATGCGGCC TTGCAGGCCA AGAGGAGCCG TGTGTCAGAC 
CCCATCAGCA GCTCAGAAAG CTCAGAAGAG GAGGAGGAAG AGGAGGAGGC AGAAGCCAAA 
ACCACCAAAC CCACCCCGAG ATTAGCAGCT ACCAACTCCT CAGTCACAGG GACAGTTTCG 
TCTTCAAATG TGAAAGAAAA AGCCAAGGCA AAGACCACGA AAGCCAGCAA GACGGTGAAC 
TCCACGACAC ACCCTGCCCC GGGAAAGGCA GTGGCCCACC TCCTCACTGG GAAGTCACCC 
CAGAAGACAT CGGGGCCCTC GGCAAATGCT GTCTTGGTGT CAGAAACCGA GGAGGAGGGC 
AGCGTGTCAG CCCTCAGAAC CGCCACCACG CCTGGAATGG CATCCGCCGA CCAGGCTGAC 
AGCTCCAGTG AGGATACCAC CAGCTCGAGT GATGAGACGG ACTTGGAGGC GAAAGCCGCA 
GTAAAACCAC TGCAGGTCAG AGCCTCAGCC ACTGCAGTCA AGGAGTCTCC GAGAAAAAGT 
GCAGCCCCGA CTCCAGGGAA GGCAGGGGTT GTGACACCCC AAGTCAGAGG CGGTGCTGTG 
ACCCCAGCCA GCAGGGCCAA GAAGCCAGAA GAGGTCTCAG AAAGCAGTGA AGAGTCCGAC 
GAGAGTGAGG AGGCCGCCCC TGCGGGGACA CCCAGTCAGG TGAAGGCCTT GGAGAAAACC 
GTCCCAATCA GAGTTGCTCC TAGTCCTGCC AAGGGGACCC CTGGGAAAGG GGCCACCCCA 
GCACCCCCTG GGAAGGCAGG GCCCTTAGGG AAGCCAGAGG AGGACTCGGA GAGCAGCAGC 
GAGGAGTCAG ACAGCGAGGA GGAGACACAA GCTGTCAAGA CCCCAGTGCA GGCAAAGCCC 
TCTGGGAAGA TCCCCCAGGT TAAAGCTGCC TCCACACCAA AAATCCAGGT GTTTTTCCCT 
AAGAAAGGGT CCCCTCCAAC ATCCCCTGGC AAGGCAGGGC CTGCAGCCAC CCAAGCCCAG 
CCCAGGAAGC AGGAGGAGGA GGAGAGCAGC AGCAGCAGCA GCAAGGAATC AGACAGCGAG 
GGCGAGGTGC CCACGGCCAT GCAGCAGACT ACAAGCCTGG CTCAGGCAAA GCCCTTGGAG 
AAAAACTCTC AGGTCAGAGC TGCCTCAGCC ACAGGTGCAG GACCCTCGGC AAAGGGCACG 
GTCCCAGCGC TCCCTCAGAA GGCAAGGTCT GTGGCCGCCC AGGATGGGAA GCAGGAGGAC 
TCAGAGAGCA GCAGTGAGGA GGAGTCAGAC AGCGAGGAGG AGATGCAGAA GGCAGGGACC 
TCAGCCCAGG CACAGTCCTC TGGGAAAGCC CTCCAGATCA GACCTGCCTC CGGTCCCACC 
AAGAGGCCCC CTCAGAAGGC CGGGCCTGCA ACCACCCAGG TCAAGGTTGA AAGGGCCGAG 
GAAGACTCGG AGAGCAGTGA GGAGGAATCA GACAGTGAGG ATGAAGCTCC TGTAGCCAAG 
ACTCCAGCCC AGGCAAAATC AGCTGTGAAA ACTTCTCAGA TCAAGGCCTC CCCAAGGAAG 
GGCACCCCCA TTACACCCTC ATCTGCCAGG GTCCCCCCAG TGCAAGTCGG CACACCAGCC 
CCCCGGACAG CAGCAGCAGT GAGTACGCCC ACCTGCGCGT CATCCCCAGC CATAGTCAGG 
CACACCCAGA AGCCAGGGGA GGACTCTTCA AGCAGCGAGG AGTCCGAGAG CGAGGAGGAG 
ACAGCTCCTG CTGCAGCAGG GGGACAGATG AAGTCTATAG GGAAGACCCT CCCAGTGAAA 
GCTGCCTCAC CAGCCACCAA GGGGCCCTCA GGAAAAGGGA CCACCCCAGG GCCCCCTGGG 
AAGGCAGGAC CTGCAGTGGC ACAGGTCAAG GCTGAGGTGC AGGAAGACTC AGAAAGCAGT 
GAGGAGTCGG ACAATGAGGA AGCGGCTGCA ACTCCCGCAC AGGTGAAGAC TTCAGTGAAA 
ACCCCTCAGA CCAAAGCCAA CTCTTCTGCC ACCAGAGTGG CTTCAGCCAA AGCAGCAGCA 
CCAGCTCCTG GAAAGGGGGG CCTTGCTGTT GCTCAAGCCA AAGTGGGGTC CCCAGCCAAG 
GTAAAACCAC CAGCGAGAAC CCCCCAGAGT AGCGCCGTCT CTGGGAGGGG CCAGGTGTCT 
GTGCCGGCTG TGGGGAAGGC AGCAGCTGCA GCAGCCCAGG CCCAGCCGGG GCCTGTCAAG 
GGCTCACAGG AGGACTCGGA GAGCAGTGAG GAGGAGTCGG ACAGTGAGGG AGAGGCACCT 
CCTCAGGGGA AGCCCTCAGG AAAGACCCCC CAGGTCAGAA CTGCCTCAGC CCCCACCAAG 
GCGTCCCCCA AGAAAGGGGC TGCCCCAGTA CCCCCTGGGA AAGCAGGACC CCCAGCCACC 
CAGGCCAAGA GGCAGGAGGA GGACTCGGAG AGCAGCAGCG AGGAGGAGTC GGACAGTGAC 
GGGGACGTGC CAGCTGCTGC ACCTCCAGCC CAGAAAGAGG GTAGCTCCAA AACTGCCACA 
AGCAAGACCC TGGCCCCAGC GTCCCTGGAG AAGAAAGCAG AGGAGTCCTC AACCAGCAGT 
GATGAGGACC TGCCATCCAG CCAGGTGATT AAAACCCCTC TGATTTTTGT CGACCCTAAT 
CGTAGTCCAG CTGGTCCAGC TGCTACCCCC GCCCAAGCGC AGGCTACAGG CACCCTGAGG 
AAGGCTCAGG CCTTGGAGAG CACAGCCAGG AGCTCCTCTG AGAGCGAGGA CGAGGACGTG 
ATCCCCGCTA CACAGTGCCC GACTCCTGCC ACTAGGACCA GCGTGGTGAC TGTGCCCACG 
GCACCCCCAA AGGCGGCCCT CAGAGCCAGT GTGGTGGGGG CTCCCAGCAG TGAGGACACA 
AGTCAGGGGT CTGAAGGCAA GAAGCAGGAG GCCTCAGCCA CTCAGGTGAC CAAGAGGAAC 
CTCCCGCTGA CACAGGCTGC CCTGAAGGTT CTTGCCCAGA AAGCCAGTGA GGCCCAATCT 
CCTGCTGCCA AGACCCCGTC TTCAAGTGGG ACTGACAGTG CTGTGGGAAC ACTCTCCACG 
GCAAGTCCCC AGAGCACCCC TACCCAGGTC AGAATGGCCA ACAAGCTCAG AAAGCCTGAG 
GTTCCTACAG CTCAGCAGGC CACAGCCACT CCCTCGGGAC ACCCCACAGC CAAGGCCCCT 
GCAACCTCAG ATGACAGCAA CAGCAGCTCT TCAGGGAGCG AGGACGATGC CAAGGGGTCC 
CAGGCGGCCC CGTCAGCCCA CAGGCCAGGC CCAGCCCCCT CCAGGAGGGA GACCTTGGTG 
GAAGAGACCA CAACAGAGTC CAGTGACGAC GAGGTGGTGG CACCTTCCCA GTCTCTCCTC 
TCAGGTTTTG TGACTCCTGG GCTGACTCCA GCTGGTTCCA AGACTTCAAA GGCCGCTCCC 
AAACCAGACG CCAGCCCCTC TGTTTCCTCT ACTCCGGCCA CCAGAGATGC CCCAGAGGGC 
AAGCAGGAAG TGGAGCCCCA ACAAGCAGCA GGCACCATGT CCCCTAAAAC AGGCAGAAAA 
GAGGCTGGTG CCACACCTCA GAAGCCCGGG AAGGGGCTCG GGAGTCCCCC GGCCTCCATG 
CTGGCGCTGC AGAGCAACAT CACCCAGCGC CTCCTGAGCG AGCCCTGGCC CCTGAGCGAG 
GCGCAGGTGC AGGCCTCGGT GGCGAAGGTG CTCACGGAGC TGCTGGAGCA GGAGAGGAAG 
AAGGCCATGG ACACCGCCAA GGAAGGCAGC CGGAAGGGCC GCCTGGGCCA TAAGCGGAAG 
CTGTCCGAAG ACCAGACAGC CCCCAAGGCC CCCAAGAACA AGAAAAAGAA GCAGCTGGCA 
GCTGCAGATG GTGGGGAGCA TGTCGTCTCC TCAGAAAAGG CCCCCAGGAC TGTCAAAGGG 
AAATCCAAGA AGGACAGAGC AAGTGGTGAC GTCAAGGAGA AGAAGGAGAA GGAGTCTCCC 
GGCTCTCAAG GGGCCAAGGA GAAGCCAGTA GGGGAGCTGG GGACTCCAAA GGGTGACGGG 
GGAGACCACG GCAACCTTAA GATGAAGAAA GAGAAGAAGA AATCTGACAA GAAAAAAAAA 
GACAAGGAGA AAAAGGAAAA GAAGAAGAAA GCAAAAAAGG CCTCAACCAA AGACCCTGGC 
TCACCATCAC AGAAGAAAAA GAAGAGAAAG AAGAAGACAG CAGAGCAAAC CGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_002689293.6 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002689339.2 Bos taurus treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X1, mRNA.

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CloneID	OBa236764
Clone ID Related Accession (Same CDS sequence)	XM_010807517.3 , XM_002689293.6
Accession Version	XM_002689293.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4377bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	treacle protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002689293.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAGG CCAGGAAGCG GCGAGAGCTC CTTCCCCTGA TCTACCAGCA TCTGCTGCAG 
GCGGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA AATTTTCCTG 
AGTCAGCCTG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACTTC GGAGATTGGC 
CGGAAACGGA AGGCAGAGGA AGATGCGGCC TTGCAGGCCA AGAGGAGCCG TGTGTCAGAC 
CCCATCAGCA GCTCAGAAAG CTCAGAAGAG GAGGAGGAAG AGGAGGAGGC AGAAGCCAAA 
ACCACCAAAC CCACCCCGAG ATTAGCAGCT ACCAACTCCT CAGTCACAGG GACAGTTTCG 
TCTTCAAATG TGAAAGAAAA AGCCAAGGCA AAGACCACGA AAGCCAGCAA GACGGTGAAC 
TCCACGACAC ACCCTGCCCC GGGAAAGGCA GTGGCCCACC TCCTCACTGG GAAGTCACCC 
CAGAAGACAT CGGGGCCCTC GGCAAATGCT GTCTTGGTGT CAGAAACCGA GGAGGAGGGC 
AGCGTGTCAG CCCTCAGAAC CGCCACCACG CCTGGAATGG CATCCGCCGA CCAGGCTGAC 
AGCTCCAGTG AGGATACCAC CAGCTCGAGT GATGAGACGG ACTTGGAGGC GAAAGCCGCA 
GTAAAACCAC TGCAGGTCAG AGCCTCAGCC ACTGCAGTCA AGGAGTCTCC GAGAAAAAGT 
GCAGCCCCGA CTCCAGGGAA GGCAGGGGTT GTGACACCCC AAGTCAGAGG CGGTGCTGTG 
ACCCCAGCCA GCAGGGCCAA GAAGCCAGAA GAGGTCTCAG AAAGCAGTGA AGAGTCCGAC 
GAGAGTGAGG AGGCCGCCCC TGCGGGGACA CCCAGTCAGG TGAAGGCCTT GGAGAAAACC 
GTCCCAATCA GAGTTGCTCC TAGTCCTGCC AAGGGGACCC CTGGGAAAGG GGCCACCCCA 
GCACCCCCTG GGAAGGCAGG GCCCTTAGGG AAGCCAGAGG AGGACTCGGA GAGCAGCAGC 
GAGGAGTCAG ACAGCGAGGA GGAGACACAA GCTGTCAAGA CCCCAGTGCA GGCAAAGCCC 
TCTGGGAAGA TCCCCCAGGT TAAAGCTGCC TCCACACCAA AAATCCAGGT GTTTTTCCCT 
AAGAAAGGGT CCCCTCCAAC ATCCCCTGGC AAGGCAGGGC CTGCAGCCAC CCAAGCCCAG 
CCCAGGAAGC AGGAGGAGGA GGAGAGCAGC AGCAGCAGCA GCAAGGAATC AGACAGCGAG 
GGCGAGGTGC CCACGGCCAT GCAGCAGACT ACAAGCCTGG CTCAGGCAAA GCCCTTGGAG 
AAAAACTCTC AGGTCAGAGC TGCCTCAGCC ACAGGTGCAG GACCCTCGGC AAAGGGCACG 
GTCCCAGCGC TCCCTCAGAA GGCAAGGTCT GTGGCCGCCC AGGATGGGAA GCAGGAGGAC 
TCAGAGAGCA GCAGTGAGGA GGAGTCAGAC AGCGAGGAGG AGATGCAGAA GGCAGGGACC 
TCAGCCCAGG CACAGTCCTC TGGGAAAGCC CTCCAGATCA GACCTGCCTC CGGTCCCACC 
AAGAGGCCCC CTCAGAAGGC CGGGCCTGCA ACCACCCAGG TCAAGGTTGA AAGGGCCGAG 
GAAGACTCGG AGAGCAGTGA GGAGGAATCA GACAGTGAGG ATGAAGCTCC TGTAGCCAAG 
ACTCCAGCCC AGGCAAAATC AGCTGTGAAA ACTTCTCAGA TCAAGGCCTC CCCAAGGAAG 
GGCACCCCCA TTACACCCTC ATCTGCCAGG GTCCCCCCAG TGCAAGTCGG CACACCAGCC 
CCCCGGACAG CAGCAGCAGT GAGTACGCCC ACCTGCGCGT CATCCCCAGC CATAGTCAGG 
CACACCCAGA AGCCAGGGGA GGACTCTTCA AGCAGCGAGG AGTCCGAGAG CGAGGAGGAG 
ACAGCTCCTG CTGCAGCAGG GGGACAGATG AAGTCTATAG GGAAGACCCT CCCAGTGAAA 
GCTGCCTCAC CAGCCACCAA GGGGCCCTCA GGAAAAGGGA CCACCCCAGG GCCCCCTGGG 
AAGGCAGGAC CTGCAGTGGC ACAGGTCAAG GCTGAGGTGC AGGAAGACTC AGAAAGCAGT 
GAGGAGTCGG ACAATGAGGA AGCGGCTGCA ACTCCCGCAC AGGTGAAGAC TTCAGTGAAA 
ACCCCTCAGA CCAAAGCCAA CTCTTCTGCC ACCAGAGTGG CTTCAGCCAA AGCAGCAGCA 
CCAGCTCCTG GAAAGGGGGG CCTTGCTGTT GCTCAAGCCA AAGTGGGGTC CCCAGCCAAG 
GTAAAACCAC CAGCGAGAAC CCCCCAGAGT AGCGCCGTCT CTGGGAGGGG CCAGGTGTCT 
GTGCCGGCTG TGGGGAAGGC AGCAGCTGCA GCAGCCCAGG CCCAGCCGGG GCCTGTCAAG 
GGCTCACAGG AGGACTCGGA GAGCAGTGAG GAGGAGTCGG ACAGTGAGGG AGAGGCACCT 
CCTCAGGGGA AGCCCTCAGG AAAGACCCCC CAGGTCAGAA CTGCCTCAGC CCCCACCAAG 
GCGTCCCCCA AGAAAGGGGC TGCCCCAGTA CCCCCTGGGA AAGCAGGACC CCCAGCCACC 
CAGGCCAAGA GGCAGGAGGA GGACTCGGAG AGCAGCAGCG AGGAGGAGTC GGACAGTGAC 
GGGGACGTGC CAGCTGCTGC ACCTCCAGCC CAGAAAGAGG GTAGCTCCAA AACTGCCACA 
AGCAAGACCC TGGCCCCAGC GTCCCTGGAG AAGAAAGCAG AGGAGTCCTC AACCAGCAGT 
GATGAGGACC TGCCATCCAG CCAGGTGATT AAAACCCCTC TGATTTTTGT CGACCCTAAT 
CGTAGTCCAG CTGGTCCAGC TGCTACCCCC GCCCAAGCGC AGGCTACAGG CACCCTGAGG 
AAGGCTCAGG CCTTGGAGAG CACAGCCAGG AGCTCCTCTG AGAGCGAGGA CGAGGACGTG 
ATCCCCGCTA CACAGTGCCC GACTCCTGCC ACTAGGACCA GCGTGGTGAC TGTGCCCACG 
GCACCCCCAA AGGCGGCCCT CAGAGCCAGT GTGGTGGGGG CTCCCAGCAG TGAGGACACA 
AGTCAGGGGT CTGAAGGCAA GAAGCAGGAG GCCTCAGCCA CTCAGGTGAC CAAGAGGAAC 
CTCCCGCTGA CACAGGCTGC CCTGAAGGTT CTTGCCCAGA AAGCCAGTGA GGCCCAATCT 
CCTGCTGCCA AGACCCCGTC TTCAAGTGGG ACTGACAGTG CTGTGGGAAC ACTCTCCACG 
GCAAGTCCCC AGAGCACCCC TACCCAGGTC AGAATGGCCA ACAAGCTCAG AAAGCCTGAG 
GTTCCTACAG CTCAGCAGGC CACAGCCACT CCCTCGGGAC ACCCCACAGC CAAGGCCCCT 
GCAACCTCAG ATGACAGCAA CAGCAGCTCT TCAGGGAGCG AGGACGATGC CAAGGGGTCC 
CAGGCGGCCC CGTCAGCCCA CAGGCCAGGC CCAGCCCCCT CCAGGAGGGA GACCTTGGTG 
GAAGAGACCA CAACAGAGTC CAGTGACGAC GAGGTGGTGG CACCTTCCCA GTCTCTCCTC 
TCAGGTTTTG TGACTCCTGG GCTGACTCCA GCTGGTTCCA AGACTTCAAA GGCCGCTCCC 
AAACCAGACG CCAGCCCCTC TGTTTCCTCT ACTCCGGCCA CCAGAGATGC CCCAGAGGGC 
AAGCAGGAAG TGGAGCCCCA ACAAGCAGCA GGCACCATGT CCCCTAAAAC AGGCAGAAAA 
GAGGCTGGTG CCACACCTCA GAAGCCCGGG AAGGGGCTCG GGAGTCCCCC GGCCTCCATG 
CTGGCGCTGC AGAGCAACAT CACCCAGCGC CTCCTGAGCG AGCCCTGGCC CCTGAGCGAG 
GCGCAGGTGC AGGCCTCGGT GGCGAAGGTG CTCACGGAGC TGCTGGAGCA GGAGAGGAAG 
AAGGCCATGG ACACCGCCAA GGAAGGCAGC CGGAAGGGCC GCCTGGGCCA TAAGCGGAAG 
CTGTCCGAAG ACCAGACAGC CCCCAAGGCC CCCAAGAACA AGAAAAAGAA GCAGCTGGCA 
GCTGCAGATG GTGGGGAGCA TGTCGTCTCC TCAGAAAAGG CCCCCAGGAC TGTCAAAGGG 
AAATCCAAGA AGGACAGAGC AAGTGGTGAC GTCAAGGAGA AGAAGGAGAA GGAGTCTCCC 
GGCTCTCAAG GGGCCAAGGA GAAGCCAGTA GGGGAGCTGG GGACTCCAAA GGGTGACGGG 
GGAGACCACG GCAACCTTAA GATGAAGAAA GAGAAGAAGA AATCTGACAA GAAAAAAAAA 
GACAAGGAGA AAAAGGAAAA GAAGAAGAAA GCAAAAAAGG CCTCAACCAA AGACCCTGGC 
TCACCATCAC AGAAGAAAAA GAAGAGAAAG AAGAAGACAG CAGAGCAAAC CGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002689339.2
CDS	97..4473
Translation	MAEARKRRELLPLIYQHLLQAGYVRAAREVKEQSGQKIFLSQPVTLLDIYTHWQQTSEIGRKRKAEEDAALQAKRSRVSDPISSSESSEEEEEEEEAEAKTTKPTPRLAATNSSVTGTVSSSNVKEKAKAKTTKASKTVNSTTHPAPGKAVAHLLTGKSPQKTSGPSANAVLVSETEEEGSVSALRTATTPGMASADQADSSSEDTTSSSDETDLEAKAAVKPLQVRASATAVKESPRKSAAPTPGKAGVVTPQVRGGAVTPASRAKKPEEVSESSEESDESEEAAPAGTPSQVKALEKTVPIRVAPSPAKGTPGKGATPAPPGKAGPLGKPEEDSESSSEESDSEEETQAVKTPVQAKPSGKIPQVKAASTPKIQVFFPKKGSPPTSPGKAGPAATQAQPRKQEEEESSSSSSKESDSEGEVPTAMQQTTSLAQAKPLEKNSQVRAASATGAGPSAKGTVPALPQKARSVAAQDGKQEDSESSSEEESDSEEEMQKAGTSAQAQSSGKALQIRPASGPTKRPPQKAGPATTQVKVERAEEDSESSEEESDSEDEAPVAKTPAQAKSAVKTSQIKASPRKGTPITPSSARVPPVQVGTPAPRTAAAVSTPTCASSPAIVRHTQKPGEDSSSSEESESEEETAPAAAGGQMKSIGKTLPVKAASPATKGPSGKGTTPGPPGKAGPAVAQVKAEVQEDSESSEESDNEEAAATPAQVKTSVKTPQTKANSSATRVASAKAAAPAPGKGGLAVAQAKVGSPAKVKPPARTPQSSAVSGRGQVSVPAVGKAAAAAAQAQPGPVKGSQEDSESSEEESDSEGEAPPQGKPSGKTPQVRTASAPTKASPKKGAAPVPPGKAGPPATQAKRQEEDSESSSEEESDSDGDVPAAAPPAQKEGSSKTATSKTLAPASLEKKAEESSTSSDEDLPSSQVIKTPLIFVDPNRSPAGPAATPAQAQATGTLRKAQALESTARSSSESEDEDVIPATQCPTPATRTSVVTVPTAPPKAALRASVVGAPSSEDTSQGSEGKKQEASATQVTKRNLPLTQAALKVLAQKASEAQSPAAKTPSSSGTDSAVGTLSTASPQSTPTQVRMANKLRKPEVPTAQQATATPSGHPTAKAPATSDDSNSSSSGSEDDAKGSQAAPSAHRPGPAPSRRETLVEETTTESSDDEVVAPSQSLLSGFVTPGLTPAGSKTSKAAPKPDASPSVSSTPATRDAPEGKQEVEPQQAAGTMSPKTGRKEAGATPQKPGKGLGSPPASMLALQSNITQRLLSEPWPLSEAQVQASVAKVLTELLEQERKKAMDTAKEGSRKGRLGHKRKLSEDQTAPKAPKNKKKKQLAAADGGEHVVSSEKAPRTVKGKSKKDRASGDVKEKKEKESPGSQGAKEKPVGELGTPKGDGGDHGNLKMKKEKKKSDKKKKDKEKKEKKKKAKKASTKDPGSPSQKKKKRKKKTAEQTV

Target ORF information:

RefSeq Version	XM_002689293.6
Organism	Bos taurus(cattle)
Definition	Bos taurus treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002689293.6

ORF Insert Sequence:

ATGGCTGAGG CCAGGAAGCG GCGAGAGCTC CTTCCCCTGA TCTACCAGCA TCTGCTGCAG 
GCGGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA AATTTTCCTG 
AGTCAGCCTG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACTTC GGAGATTGGC 
CGGAAACGGA AGGCAGAGGA AGATGCGGCC TTGCAGGCCA AGAGGAGCCG TGTGTCAGAC 
CCCATCAGCA GCTCAGAAAG CTCAGAAGAG GAGGAGGAAG AGGAGGAGGC AGAAGCCAAA 
ACCACCAAAC CCACCCCGAG ATTAGCAGCT ACCAACTCCT CAGTCACAGG GACAGTTTCG 
TCTTCAAATG TGAAAGAAAA AGCCAAGGCA AAGACCACGA AAGCCAGCAA GACGGTGAAC 
TCCACGACAC ACCCTGCCCC GGGAAAGGCA GTGGCCCACC TCCTCACTGG GAAGTCACCC 
CAGAAGACAT CGGGGCCCTC GGCAAATGCT GTCTTGGTGT CAGAAACCGA GGAGGAGGGC 
AGCGTGTCAG CCCTCAGAAC CGCCACCACG CCTGGAATGG CATCCGCCGA CCAGGCTGAC 
AGCTCCAGTG AGGATACCAC CAGCTCGAGT GATGAGACGG ACTTGGAGGC GAAAGCCGCA 
GTAAAACCAC TGCAGGTCAG AGCCTCAGCC ACTGCAGTCA AGGAGTCTCC GAGAAAAAGT 
GCAGCCCCGA CTCCAGGGAA GGCAGGGGTT GTGACACCCC AAGTCAGAGG CGGTGCTGTG 
ACCCCAGCCA GCAGGGCCAA GAAGCCAGAA GAGGTCTCAG AAAGCAGTGA AGAGTCCGAC 
GAGAGTGAGG AGGCCGCCCC TGCGGGGACA CCCAGTCAGG TGAAGGCCTT GGAGAAAACC 
GTCCCAATCA GAGTTGCTCC TAGTCCTGCC AAGGGGACCC CTGGGAAAGG GGCCACCCCA 
GCACCCCCTG GGAAGGCAGG GCCCTTAGGG AAGCCAGAGG AGGACTCGGA GAGCAGCAGC 
GAGGAGTCAG ACAGCGAGGA GGAGACACAA GCTGTCAAGA CCCCAGTGCA GGCAAAGCCC 
TCTGGGAAGA TCCCCCAGGT TAAAGCTGCC TCCACACCAA AAATCCAGGT GTTTTTCCCT 
AAGAAAGGGT CCCCTCCAAC ATCCCCTGGC AAGGCAGGGC CTGCAGCCAC CCAAGCCCAG 
CCCAGGAAGC AGGAGGAGGA GGAGAGCAGC AGCAGCAGCA GCAAGGAATC AGACAGCGAG 
GGCGAGGTGC CCACGGCCAT GCAGCAGACT ACAAGCCTGG CTCAGGCAAA GCCCTTGGAG 
AAAAACTCTC AGGTCAGAGC TGCCTCAGCC ACAGGTGCAG GACCCTCGGC AAAGGGCACG 
GTCCCAGCGC TCCCTCAGAA GGCAAGGTCT GTGGCCGCCC AGGATGGGAA GCAGGAGGAC 
TCAGAGAGCA GCAGTGAGGA GGAGTCAGAC AGCGAGGAGG AGATGCAGAA GGCAGGGACC 
TCAGCCCAGG CACAGTCCTC TGGGAAAGCC CTCCAGATCA GACCTGCCTC CGGTCCCACC 
AAGAGGCCCC CTCAGAAGGC CGGGCCTGCA ACCACCCAGG TCAAGGTTGA AAGGGCCGAG 
GAAGACTCGG AGAGCAGTGA GGAGGAATCA GACAGTGAGG ATGAAGCTCC TGTAGCCAAG 
ACTCCAGCCC AGGCAAAATC AGCTGTGAAA ACTTCTCAGA TCAAGGCCTC CCCAAGGAAG 
GGCACCCCCA TTACACCCTC ATCTGCCAGG GTCCCCCCAG TGCAAGTCGG CACACCAGCC 
CCCCGGACAG CAGCAGCAGT GAGTACGCCC ACCTGCGCGT CATCCCCAGC CATAGTCAGG 
CACACCCAGA AGCCAGGGGA GGACTCTTCA AGCAGCGAGG AGTCCGAGAG CGAGGAGGAG 
ACAGCTCCTG CTGCAGCAGG GGGACAGATG AAGTCTATAG GGAAGACCCT CCCAGTGAAA 
GCTGCCTCAC CAGCCACCAA GGGGCCCTCA GGAAAAGGGA CCACCCCAGG GCCCCCTGGG 
AAGGCAGGAC CTGCAGTGGC ACAGGTCAAG GCTGAGGTGC AGGAAGACTC AGAAAGCAGT 
GAGGAGTCGG ACAATGAGGA AGCGGCTGCA ACTCCCGCAC AGGTGAAGAC TTCAGTGAAA 
ACCCCTCAGA CCAAAGCCAA CTCTTCTGCC ACCAGAGTGG CTTCAGCCAA AGCAGCAGCA 
CCAGCTCCTG GAAAGGGGGG CCTTGCTGTT GCTCAAGCCA AAGTGGGGTC CCCAGCCAAG 
GTAAAACCAC CAGCGAGAAC CCCCCAGAGT AGCGCCGTCT CTGGGAGGGG CCAGGTGTCT 
GTGCCGGCTG TGGGGAAGGC AGCAGCTGCA GCAGCCCAGG CCCAGCCGGG GCCTGTCAAG 
GGCTCACAGG AGGACTCGGA GAGCAGTGAG GAGGAGTCGG ACAGTGAGGG AGAGGCACCT 
CCTCAGGGGA AGCCCTCAGG AAAGACCCCC CAGGTCAGAA CTGCCTCAGC CCCCACCAAG 
GCGTCCCCCA AGAAAGGGGC TGCCCCAGTA CCCCCTGGGA AAGCAGGACC CCCAGCCACC 
CAGGCCAAGA GGCAGGAGGA GGACTCGGAG AGCAGCAGCG AGGAGGAGTC GGACAGTGAC 
GGGGACGTGC CAGCTGCTGC ACCTCCAGCC CAGAAAGAGG GTAGCTCCAA AACTGCCACA 
AGCAAGACCC TGGCCCCAGC GTCCCTGGAG AAGAAAGCAG AGGAGTCCTC AACCAGCAGT 
GATGAGGACC TGCCATCCAG CCAGGTGATT AAAACCCCTC TGATTTTTGT CGACCCTAAT 
CGTAGTCCAG CTGGTCCAGC TGCTACCCCC GCCCAAGCGC AGGCTACAGG CACCCTGAGG 
AAGGCTCAGG CCTTGGAGAG CACAGCCAGG AGCTCCTCTG AGAGCGAGGA CGAGGACGTG 
ATCCCCGCTA CACAGTGCCC GACTCCTGCC ACTAGGACCA GCGTGGTGAC TGTGCCCACG 
GCACCCCCAA AGGCGGCCCT CAGAGCCAGT GTGGTGGGGG CTCCCAGCAG TGAGGACACA 
AGTCAGGGGT CTGAAGGCAA GAAGCAGGAG GCCTCAGCCA CTCAGGTGAC CAAGAGGAAC 
CTCCCGCTGA CACAGGCTGC CCTGAAGGTT CTTGCCCAGA AAGCCAGTGA GGCCCAATCT 
CCTGCTGCCA AGACCCCGTC TTCAAGTGGG ACTGACAGTG CTGTGGGAAC ACTCTCCACG 
GCAAGTCCCC AGAGCACCCC TACCCAGGTC AGAATGGCCA ACAAGCTCAG AAAGCCTGAG 
GTTCCTACAG CTCAGCAGGC CACAGCCACT CCCTCGGGAC ACCCCACAGC CAAGGCCCCT 
GCAACCTCAG ATGACAGCAA CAGCAGCTCT TCAGGGAGCG AGGACGATGC CAAGGGGTCC 
CAGGCGGCCC CGTCAGCCCA CAGGCCAGGC CCAGCCCCCT CCAGGAGGGA GACCTTGGTG 
GAAGAGACCA CAACAGAGTC CAGTGACGAC GAGGTGGTGG CACCTTCCCA GTCTCTCCTC 
TCAGGTTTTG TGACTCCTGG GCTGACTCCA GCTGGTTCCA AGACTTCAAA GGCCGCTCCC 
AAACCAGACG CCAGCCCCTC TGTTTCCTCT ACTCCGGCCA CCAGAGATGC CCCAGAGGGC 
AAGCAGGAAG TGGAGCCCCA ACAAGCAGCA GGCACCATGT CCCCTAAAAC AGGCAGAAAA 
GAGGCTGGTG CCACACCTCA GAAGCCCGGG AAGGGGCTCG GGAGTCCCCC GGCCTCCATG 
CTGGCGCTGC AGAGCAACAT CACCCAGCGC CTCCTGAGCG AGCCCTGGCC CCTGAGCGAG 
GCGCAGGTGC AGGCCTCGGT GGCGAAGGTG CTCACGGAGC TGCTGGAGCA GGAGAGGAAG 
AAGGCCATGG ACACCGCCAA GGAAGGCAGC CGGAAGGGCC GCCTGGGCCA TAAGCGGAAG 
CTGTCCGAAG ACCAGACAGC CCCCAAGGCC CCCAAGAACA AGAAAAAGAA GCAGCTGGCA 
GCTGCAGATG GTGGGGAGCA TGTCGTCTCC TCAGAAAAGG CCCCCAGGAC TGTCAAAGGG 
AAATCCAAGA AGGACAGAGC AAGTGGTGAC GTCAAGGAGA AGAAGGAGAA GGAGTCTCCC 
GGCTCTCAAG GGGCCAAGGA GAAGCCAGTA GGGGAGCTGG GGACTCCAAA GGGTGACGGG 
GGAGACCACG GCAACCTTAA GATGAAGAAA GAGAAGAAGA AATCTGACAA GAAAAAAAAA 
GACAAGGAGA AAAAGGAAAA GAAGAAGAAA GCAAAAAAGG CCTCAACCAA AGACCCTGGC 
TCACCATCAC AGAAGAAAAA GAAGAGAAAG AAGAAGACAG CAGAGCAAAC CGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_024995259.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024851027.1 Bos taurus treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa236765
Clone ID Related Accession (Same CDS sequence)	XM_024995259.1
Accession Version	XM_024995259.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4239bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	treacle protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAGG CCAGGAAGCG GCGAGAGCTC CTTCCCCTGA TCTACCAGCA TCTGCTGCAG 
GCGGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA AATTTTCCTG 
AGTCAGCCTG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACTTC GGAGATTGGC 
CGGAAACGGA AGGCAGAGGA AGATGCGGCC TTGCAGGCCA AGAGGAGCCG TGTGTCAGAC 
CCCATCAGCA GCTCAGAAAG CTCAGAAGAG GAGGAGGAAG AGGAGGAGGC AGAAGCCAAA 
ACCACCAAAC CCACCCCGAG ATTAGCAGCT ACCAACTCCT CAGTCACAGG GACAGTTTCG 
TCTTCAAATG TGAAAGAAAA AGCCAAGGCA AAGACCACGA AAGCCAGCAA GACGGTGAAC 
TCCACGACAC ACCCTGCCCC GGGAAAGGCA GTGGCCCACC TCCTCACTGG GAAGTCACCC 
CAGAAGACAT CGGGGCCCTC GGCAAATGCT GTCTTGGTGT CAGAAACCGA GGAGGAGGGC 
AGCGTGTCAG CCCTCAGAAC CGCCACCACG CCTGGAATGG CATCCGCCGA CCAGGCTGAC 
AGCTCCAGTG AGGATACCAC CAGCTCGAGT GATGAGACGG ACTTGGAGGC GAAAGCCGCA 
GTAAAACCAC TGCAGGTCAG AGCCTCAGCC ACTGCAGTCA AGGAGTCTCC GAGAAAAAGT 
GCAGCCCCGA CTCCAGGGAA GGCAGGGGTT GTGACACCCC AAGTCAGAGG CGGTGCTGTG 
ACCCCAGCCA GCAGGGCCAA GAAGCCAGAA GAGGTCTCAG AAAGCAGTGA AGAGTCCGAC 
GAGAGTGAGG AGGCCGCCCC TGCGGGGACA CCCAGTCAGG TGAAGGCCTT GGAGAAAACC 
GTCCCAATCA GAGTTGCTCC TAGTCCTGCC AAGGGGACCC CTGGGAAAGG GGCCACCCCA 
GCACCCCCTG GGAAGGCAGG GCCCTTAGGG AAGCCAGAGG AGGACTCGGA GAGCAGCAGC 
GAGGAGTCAG ACAGCGAGGA GGAGACACAA GCTGTCAAGA CCCCAGTGCA GGCAAAGCCC 
TCTGGGAAGA TCCCCCAGGT TAAAGCTGCC TCCACACCAA AAATCCAGGT GTTTTTCCCT 
AAGAAAGGGT CCCCTCCAAC ATCCCCTGGC AAGGCAGGGC CTGCAGCCAC CCAAGCCCAG 
CCCAGGAAGC AGGAGGAGGA GGAGAGCAGC AGCAGCAGCA GCAAGGAATC AGACAGCGAG 
GGCGAGGTGC CCACGGCCAT GCAGCAGACT ACAAGCCTGG CTCAGGCAAA GCCCTTGGAG 
AAAAACTCTC AGGTCAGAGC TGCCTCAGCC ACAGGTGCAG GACCCTCGGC AAAGGGCACG 
GTCCCAGCGC TCCCTCAGAA GGCAAGGTCT GTGGCCGCCC AGGATGGGAA GCAGGAGGAC 
TCAGAGAGCA GCAGTGAGGA GGAGTCAGAC AGCGAGGAGG AGATGCAGAA GGCAGGGACC 
TCAGCCCAGG CACAGTCCTC TGGGAAAGCC CTCCAGATCA GACCTGCCTC CGGTCCCACC 
AAGAGGCCCC CTCAGAAGGC CGGGCCTGCA ACCACCCAGG TCAAGGTTGA AAGGGCCGAG 
GAAGACTCGG AGAGCAGTGA GGAGGAATCA GACAGTGAGG ATGAAGCTCC TGTAGCCAAG 
ACTCCAGCCC AGGCAAAATC AGCTGTGAAA ACTTCTCAGA TCAAGGCCTC CCCAAGGAAG 
GGCACCCCCA TTACACCCTC ATCTGCCAGG GTCCCCCCAG TGCAAGTCGG CACACCAGCC 
CCCCGGACAG CAGCAGCAGT GAGTACGCCC ACCTGCGCGT CATCCCCAGC CATAGTCAGG 
CACACCCAGA AGCCAGGGGA GGACTCTTCA AGCAGCGAGG AGTCCGAGAG CGAGGAGGAG 
ACAGCTCCTG CTGCAGCAGG GGGACAGATG AAGTCTATAG GGAAGACCCT CCCAGTGAAA 
GCTGCCTCAC CAGCCACCAA GGGGCCCTCA GGAAAAGGGA CCACCCCAGG GCCCCCTGGG 
AAGGCAGGAC CTGCAGTGGC ACAGGTCAAG GCTGAGGTGC AGGAAGACTC AGAAAGCAGT 
GAGGAGTCGG ACAATGAGGA AGCGGCTGCA ACTCCCGCAC AGGTAAAACC ACCAGCGAGA 
ACCCCCCAGA GTAGCGCCGT CTCTGGGAGG GGCCAGGTGT CTGTGCCGGC TGTGGGGAAG 
GCAGCAGCTG CAGCAGCCCA GGCCCAGCCG GGGCCTGTCA AGGGCTCACA GGAGGACTCG 
GAGAGCAGTG AGGAGGAGTC GGACAGTGAG GGAGAGGCAC CTCCTCAGGG GAAGCCCTCA 
GGAAAGACCC CCCAGGTCAG AACTGCCTCA GCCCCCACCA AGGCGTCCCC CAAGAAAGGG 
GCTGCCCCAG TACCCCCTGG GAAAGCAGGA CCCCCAGCCA CCCAGGCCAA GAGGCAGGAG 
GAGGACTCGG AGAGCAGCAG CGAGGAGGAG TCGGACAGTG ACGGGGACGT GCCAGCTGCT 
GCACCTCCAG CCCAGAAAGA GGGTAGCTCC AAAACTGCCA CAAGCAAGAC CCTGGCCCCA 
GCGTCCCTGG AGAAGAAAGC AGAGGAGTCC TCAACCAGCA GTGATGAGGA CCTGCCATCC 
AGCCAGGTGA TTAAAACCCC TCTGATTTTT GTCGACCCTA ATCGTAGTCC AGCTGGTCCA 
GCTGCTACCC CCGCCCAAGC GCAGGCTACA GGCACCCTGA GGAAGGCTCA GGCCTTGGAG 
AGCACAGCCA GGAGCTCCTC TGAGAGCGAG GACGAGGACG TGATCCCCGC TACACAGTGC 
CCGACTCCTG CCACTAGGAC CAGCGTGGTG ACTGTGCCCA CGGCACCCCC AAAGGCGGCC 
CTCAGAGCCA GTGTGGTGGG GGCTCCCAGC AGTGAGGACA CAAGTCAGGG GTCTGAAGGC 
AAGAAGCAGG AGGCCTCAGC CACTCAGGTG ACCAAGAGGA ACCTCCCGCT GACACAGGCT 
GCCCTGAAGG TTCTTGCCCA GAAAGCCAGT GAGGCCCAAT CTCCTGCTGC CAAGACCCCG 
TCTTCAAGTG GGACTGACAG TGCTGTGGGA ACACTCTCCA CGGCAAGTCC CCAGAGCACC 
CCTACCCAGG TCAGAATGGC CAACAAGCTC AGAAAGCCTG AGGTTCCTAC AGCTCAGCAG 
GCCACAGCCA CTCCCTCGGG ACACCCCACA GCCAAGGCCC CTGCAACCTC AGATGACAGC 
AACAGCAGCT CTTCAGGGAG CGAGGACGAT GCCAAGGGGT CCCAGGCGGC CCCGTCAGCC 
CACAGGCCAG GCCCAGCCCC CTCCAGGAGG GAGACCTTGG TGGAAGAGAC CACAACAGAG 
TCCAGTGACG ACGAGGTGGT GGCACCTTCC CAGTCTCTCC TCTCAGGTTT TGTGACTCCT 
GGGCTGACTC CAGCTGGTTC CAAGACTTCA AAGGCCGCTC CCAAACCAGA CGCCAGCCCC 
TCTGTTTCCT CTACTCCGGC CACCAGAGAT GCCCCAGAGG GCAAGCAGGA AGTGGAGCCC 
CAACAAGCAG CAGGCACCAT GTCCCCTAAA ACAGGCAGAA AAGAGGCTGG TGCCACACCT 
CAGAAGCCCG GGAAGGGGCT CGGGAGTCCC CCGGCCTCCA TGCTGGCGCT GCAGAGCAAC 
ATCACCCAGC GCCTCCTGAG CGAGCCCTGG CCCCTGAGCG AGGCGCAGGT GCAGGCCTCG 
GTGGCGAAGG TGCTCACGGA GCTGCTGGAG CAGGAGAGGA AGAAGGCCAT GGACACCGCC 
AAGGAAGGCA GCCGGAAGGG CCGCCTGGGC CATAAGCGGA AGCTGTCCGA AGACCAGACA 
GCCCCCAAGG CCCCCAAGAA CAAGAAAAAG AAGCAGCTGG CAGCTGCAGA TGGTGGGGAG 
CATGTCGTCT CCTCAGAAAA GGCCCCCAGG ACTGTCAAAG GGAAATCCAA GAAGGACAGA 
GCAAGTGGTG ACGTCAAGGA GAAGAAGGAG AAGGAGTCTC CCGGCTCTCA AGGGGCCAAG 
GAGAAGCCAG TAGGGGAGCT GGGGACTCCA AAGGGTGACG GGGGAGACCA CGGCAACCTT 
AAGATGAAGA AAGAGAAGAA GAAATCTGAC AAGAAAAAAA AAGACAAGGA GAAAAAGGAA 
AAGAAGAAGA AAGCAAAAAA GGCCTCAACC AAAGACCCTG GCTCACCATC ACAGAAGAAA 
AAGAAGAGAA AGAAGAAGAC AGCAGAGCAA ACCGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024851027.1
CDS	60..4298
Translation	MAEARKRRELLPLIYQHLLQAGYVRAAREVKEQSGQKIFLSQPVTLLDIYTHWQQTSEIGRKRKAEEDAALQAKRSRVSDPISSSESSEEEEEEEEAEAKTTKPTPRLAATNSSVTGTVSSSNVKEKAKAKTTKASKTVNSTTHPAPGKAVAHLLTGKSPQKTSGPSANAVLVSETEEEGSVSALRTATTPGMASADQADSSSEDTTSSSDETDLEAKAAVKPLQVRASATAVKESPRKSAAPTPGKAGVVTPQVRGGAVTPASRAKKPEEVSESSEESDESEEAAPAGTPSQVKALEKTVPIRVAPSPAKGTPGKGATPAPPGKAGPLGKPEEDSESSSEESDSEEETQAVKTPVQAKPSGKIPQVKAASTPKIQVFFPKKGSPPTSPGKAGPAATQAQPRKQEEEESSSSSSKESDSEGEVPTAMQQTTSLAQAKPLEKNSQVRAASATGAGPSAKGTVPALPQKARSVAAQDGKQEDSESSSEEESDSEEEMQKAGTSAQAQSSGKALQIRPASGPTKRPPQKAGPATTQVKVERAEEDSESSEEESDSEDEAPVAKTPAQAKSAVKTSQIKASPRKGTPITPSSARVPPVQVGTPAPRTAAAVSTPTCASSPAIVRHTQKPGEDSSSSEESESEEETAPAAAGGQMKSIGKTLPVKAASPATKGPSGKGTTPGPPGKAGPAVAQVKAEVQEDSESSEESDNEEAAATPAQVKPPARTPQSSAVSGRGQVSVPAVGKAAAAAAQAQPGPVKGSQEDSESSEEESDSEGEAPPQGKPSGKTPQVRTASAPTKASPKKGAAPVPPGKAGPPATQAKRQEEDSESSSEEESDSDGDVPAAAPPAQKEGSSKTATSKTLAPASLEKKAEESSTSSDEDLPSSQVIKTPLIFVDPNRSPAGPAATPAQAQATGTLRKAQALESTARSSSESEDEDVIPATQCPTPATRTSVVTVPTAPPKAALRASVVGAPSSEDTSQGSEGKKQEASATQVTKRNLPLTQAALKVLAQKASEAQSPAAKTPSSSGTDSAVGTLSTASPQSTPTQVRMANKLRKPEVPTAQQATATPSGHPTAKAPATSDDSNSSSSGSEDDAKGSQAAPSAHRPGPAPSRRETLVEETTTESSDDEVVAPSQSLLSGFVTPGLTPAGSKTSKAAPKPDASPSVSSTPATRDAPEGKQEVEPQQAAGTMSPKTGRKEAGATPQKPGKGLGSPPASMLALQSNITQRLLSEPWPLSEAQVQASVAKVLTELLEQERKKAMDTAKEGSRKGRLGHKRKLSEDQTAPKAPKNKKKKQLAAADGGEHVVSSEKAPRTVKGKSKKDRASGDVKEKKEKESPGSQGAKEKPVGELGTPKGDGGDHGNLKMKKEKKKSDKKKKDKEKKEKKKKAKKASTKDPGSPSQKKKKRKKKTAEQTV

Target ORF information:

RefSeq Version	XM_024995259.1
Organism	Bos taurus(cattle)
Definition	Bos taurus treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024995259.1

ORF Insert Sequence:

ATGGCTGAGG CCAGGAAGCG GCGAGAGCTC CTTCCCCTGA TCTACCAGCA TCTGCTGCAG 
GCGGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA AATTTTCCTG 
AGTCAGCCTG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACTTC GGAGATTGGC 
CGGAAACGGA AGGCAGAGGA AGATGCGGCC TTGCAGGCCA AGAGGAGCCG TGTGTCAGAC 
CCCATCAGCA GCTCAGAAAG CTCAGAAGAG GAGGAGGAAG AGGAGGAGGC AGAAGCCAAA 
ACCACCAAAC CCACCCCGAG ATTAGCAGCT ACCAACTCCT CAGTCACAGG GACAGTTTCG 
TCTTCAAATG TGAAAGAAAA AGCCAAGGCA AAGACCACGA AAGCCAGCAA GACGGTGAAC 
TCCACGACAC ACCCTGCCCC GGGAAAGGCA GTGGCCCACC TCCTCACTGG GAAGTCACCC 
CAGAAGACAT CGGGGCCCTC GGCAAATGCT GTCTTGGTGT CAGAAACCGA GGAGGAGGGC 
AGCGTGTCAG CCCTCAGAAC CGCCACCACG CCTGGAATGG CATCCGCCGA CCAGGCTGAC 
AGCTCCAGTG AGGATACCAC CAGCTCGAGT GATGAGACGG ACTTGGAGGC GAAAGCCGCA 
GTAAAACCAC TGCAGGTCAG AGCCTCAGCC ACTGCAGTCA AGGAGTCTCC GAGAAAAAGT 
GCAGCCCCGA CTCCAGGGAA GGCAGGGGTT GTGACACCCC AAGTCAGAGG CGGTGCTGTG 
ACCCCAGCCA GCAGGGCCAA GAAGCCAGAA GAGGTCTCAG AAAGCAGTGA AGAGTCCGAC 
GAGAGTGAGG AGGCCGCCCC TGCGGGGACA CCCAGTCAGG TGAAGGCCTT GGAGAAAACC 
GTCCCAATCA GAGTTGCTCC TAGTCCTGCC AAGGGGACCC CTGGGAAAGG GGCCACCCCA 
GCACCCCCTG GGAAGGCAGG GCCCTTAGGG AAGCCAGAGG AGGACTCGGA GAGCAGCAGC 
GAGGAGTCAG ACAGCGAGGA GGAGACACAA GCTGTCAAGA CCCCAGTGCA GGCAAAGCCC 
TCTGGGAAGA TCCCCCAGGT TAAAGCTGCC TCCACACCAA AAATCCAGGT GTTTTTCCCT 
AAGAAAGGGT CCCCTCCAAC ATCCCCTGGC AAGGCAGGGC CTGCAGCCAC CCAAGCCCAG 
CCCAGGAAGC AGGAGGAGGA GGAGAGCAGC AGCAGCAGCA GCAAGGAATC AGACAGCGAG 
GGCGAGGTGC CCACGGCCAT GCAGCAGACT ACAAGCCTGG CTCAGGCAAA GCCCTTGGAG 
AAAAACTCTC AGGTCAGAGC TGCCTCAGCC ACAGGTGCAG GACCCTCGGC AAAGGGCACG 
GTCCCAGCGC TCCCTCAGAA GGCAAGGTCT GTGGCCGCCC AGGATGGGAA GCAGGAGGAC 
TCAGAGAGCA GCAGTGAGGA GGAGTCAGAC AGCGAGGAGG AGATGCAGAA GGCAGGGACC 
TCAGCCCAGG CACAGTCCTC TGGGAAAGCC CTCCAGATCA GACCTGCCTC CGGTCCCACC 
AAGAGGCCCC CTCAGAAGGC CGGGCCTGCA ACCACCCAGG TCAAGGTTGA AAGGGCCGAG 
GAAGACTCGG AGAGCAGTGA GGAGGAATCA GACAGTGAGG ATGAAGCTCC TGTAGCCAAG 
ACTCCAGCCC AGGCAAAATC AGCTGTGAAA ACTTCTCAGA TCAAGGCCTC CCCAAGGAAG 
GGCACCCCCA TTACACCCTC ATCTGCCAGG GTCCCCCCAG TGCAAGTCGG CACACCAGCC 
CCCCGGACAG CAGCAGCAGT GAGTACGCCC ACCTGCGCGT CATCCCCAGC CATAGTCAGG 
CACACCCAGA AGCCAGGGGA GGACTCTTCA AGCAGCGAGG AGTCCGAGAG CGAGGAGGAG 
ACAGCTCCTG CTGCAGCAGG GGGACAGATG AAGTCTATAG GGAAGACCCT CCCAGTGAAA 
GCTGCCTCAC CAGCCACCAA GGGGCCCTCA GGAAAAGGGA CCACCCCAGG GCCCCCTGGG 
AAGGCAGGAC CTGCAGTGGC ACAGGTCAAG GCTGAGGTGC AGGAAGACTC AGAAAGCAGT 
GAGGAGTCGG ACAATGAGGA AGCGGCTGCA ACTCCCGCAC AGGTAAAACC ACCAGCGAGA 
ACCCCCCAGA GTAGCGCCGT CTCTGGGAGG GGCCAGGTGT CTGTGCCGGC TGTGGGGAAG 
GCAGCAGCTG CAGCAGCCCA GGCCCAGCCG GGGCCTGTCA AGGGCTCACA GGAGGACTCG 
GAGAGCAGTG AGGAGGAGTC GGACAGTGAG GGAGAGGCAC CTCCTCAGGG GAAGCCCTCA 
GGAAAGACCC CCCAGGTCAG AACTGCCTCA GCCCCCACCA AGGCGTCCCC CAAGAAAGGG 
GCTGCCCCAG TACCCCCTGG GAAAGCAGGA CCCCCAGCCA CCCAGGCCAA GAGGCAGGAG 
GAGGACTCGG AGAGCAGCAG CGAGGAGGAG TCGGACAGTG ACGGGGACGT GCCAGCTGCT 
GCACCTCCAG CCCAGAAAGA GGGTAGCTCC AAAACTGCCA CAAGCAAGAC CCTGGCCCCA 
GCGTCCCTGG AGAAGAAAGC AGAGGAGTCC TCAACCAGCA GTGATGAGGA CCTGCCATCC 
AGCCAGGTGA TTAAAACCCC TCTGATTTTT GTCGACCCTA ATCGTAGTCC AGCTGGTCCA 
GCTGCTACCC CCGCCCAAGC GCAGGCTACA GGCACCCTGA GGAAGGCTCA GGCCTTGGAG 
AGCACAGCCA GGAGCTCCTC TGAGAGCGAG GACGAGGACG TGATCCCCGC TACACAGTGC 
CCGACTCCTG CCACTAGGAC CAGCGTGGTG ACTGTGCCCA CGGCACCCCC AAAGGCGGCC 
CTCAGAGCCA GTGTGGTGGG GGCTCCCAGC AGTGAGGACA CAAGTCAGGG GTCTGAAGGC 
AAGAAGCAGG AGGCCTCAGC CACTCAGGTG ACCAAGAGGA ACCTCCCGCT GACACAGGCT 
GCCCTGAAGG TTCTTGCCCA GAAAGCCAGT GAGGCCCAAT CTCCTGCTGC CAAGACCCCG 
TCTTCAAGTG GGACTGACAG TGCTGTGGGA ACACTCTCCA CGGCAAGTCC CCAGAGCACC 
CCTACCCAGG TCAGAATGGC CAACAAGCTC AGAAAGCCTG AGGTTCCTAC AGCTCAGCAG 
GCCACAGCCA CTCCCTCGGG ACACCCCACA GCCAAGGCCC CTGCAACCTC AGATGACAGC 
AACAGCAGCT CTTCAGGGAG CGAGGACGAT GCCAAGGGGT CCCAGGCGGC CCCGTCAGCC 
CACAGGCCAG GCCCAGCCCC CTCCAGGAGG GAGACCTTGG TGGAAGAGAC CACAACAGAG 
TCCAGTGACG ACGAGGTGGT GGCACCTTCC CAGTCTCTCC TCTCAGGTTT TGTGACTCCT 
GGGCTGACTC CAGCTGGTTC CAAGACTTCA AAGGCCGCTC CCAAACCAGA CGCCAGCCCC 
TCTGTTTCCT CTACTCCGGC CACCAGAGAT GCCCCAGAGG GCAAGCAGGA AGTGGAGCCC 
CAACAAGCAG CAGGCACCAT GTCCCCTAAA ACAGGCAGAA AAGAGGCTGG TGCCACACCT 
CAGAAGCCCG GGAAGGGGCT CGGGAGTCCC CCGGCCTCCA TGCTGGCGCT GCAGAGCAAC 
ATCACCCAGC GCCTCCTGAG CGAGCCCTGG CCCCTGAGCG AGGCGCAGGT GCAGGCCTCG 
GTGGCGAAGG TGCTCACGGA GCTGCTGGAG CAGGAGAGGA AGAAGGCCAT GGACACCGCC 
AAGGAAGGCA GCCGGAAGGG CCGCCTGGGC CATAAGCGGA AGCTGTCCGA AGACCAGACA 
GCCCCCAAGG CCCCCAAGAA CAAGAAAAAG AAGCAGCTGG CAGCTGCAGA TGGTGGGGAG 
CATGTCGTCT CCTCAGAAAA GGCCCCCAGG ACTGTCAAAG GGAAATCCAA GAAGGACAGA 
GCAAGTGGTG ACGTCAAGGA GAAGAAGGAG AAGGAGTCTC CCGGCTCTCA AGGGGCCAAG 
GAGAAGCCAG TAGGGGAGCT GGGGACTCCA AAGGGTGACG GGGGAGACCA CGGCAACCTT 
AAGATGAAGA AAGAGAAGAA GAAATCTGAC AAGAAAAAAA AAGACAAGGA GAAAAAGGAA 
AAGAAGAAGA AAGCAAAAAA GGCCTCAACC AAAGACCCTG GCTCACCATC ACAGAAGAAA 
AAGAAGAGAA AGAAGAAGAC AGCAGAGCAA ACCGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_005209634.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005209691.1 Bos taurus treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa236766
Clone ID Related Accession (Same CDS sequence)	XM_005209634.4
Accession Version	XM_005209634.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4056bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	treacle protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005209634.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAGG CCAGGAAGCG GCGAGAGCTC CTTCCCCTGA TCTACCAGCA TCTGCTGCAG 
GCGGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA AATTTTCCTG 
AGTCAGCCTG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACTTC GGAGATTGGC 
CGGAAACGGA AGGCAGAGGA AGATGCGGCC TTGCAGGCCA AGAGGAGCCG TGTGTCAGAC 
CCCATCAGCA GCTCAGAAAG CTCAGAAGAG GAGGAGGAAG AGGAGGAGGC AGAAGCCAAA 
ACCACCAAAC CCACCCCGAG ATTAGCAGCT ACCAACTCCT CAGTCACAGG GACAGTTTCG 
TCTTCAAATG TGAAAGAAAA AGCCAAGGCA AAGACCACGA AAGCCAGCAA GACGGTGAAC 
TCCACGACAC ACCCTGCCCC GGGAAAGGCA GTGGCCCACC TCCTCACTGG GAAGTCACCC 
CAGAAGACAT CGGGGCCCTC GGCAAATGCT GTCTTGGTGT CAGAAACCGA GGAGGAGGGC 
AGCGTGTCAG CCCTCAGAAC CGCCACCACG CCTGGAATGG CATCCGCCGA CCAGGCTGAC 
AGCTCCAGTG AGGATACCAC CAGCTCGAGT GATGAGACGG ACTTGGAGGC GAAAGCCGCA 
GTAAAACCAC TGCAGGTCAG AGCCTCAGCC ACTGCAGTCA AGGAGTCTCC GAGAAAAAGT 
GCAGCCCCGA CTCCAGGGAA GGCAGGGGTT GTGACACCCC AAGTCAGAGG CGGTGCTGTG 
ACCCCAGCCA GCAGGGCCAA GAAGCCAGAA GAGGTCTCAG AAAGCAGTGA AGAGTCCGAC 
GAGAGTGAGG AGGCCGCCCC TGCGGGGACA CCCAGTCAGG TGAAGGCCTT GGAGAAAACC 
GTCCCAATCA GAGTTGCTCC TAGTCCTGCC AAGGGGACCC CTGGGAAAGG GGCCACCCCA 
GCACCCCCTG GGAAGGCAGG GCCCTTAGGG AAGCCAGAGG AGGACTCGGA GAGCAGCAGC 
GAGGAGTCAG ACAGCGAGGA GGAGACACAA GCTGTCAAGA CCCCAGTGCA GGCAAAGCCC 
TCTGGGAAGA TCCCCCAGGT TAAAGCTGCC TCCACACCAA AAATCCAGGT GTTTTTCCCT 
AAGAAAGGGT CCCCTCCAAC ATCCCCTGGC AAGGCAGGGC CTGCAGCCAC CCAAGCCCAG 
CCCAGGAAGC AGGAGGAGGA GGAGAGCAGC AGCAGCAGCA GCAAGGAATC AGACAGCGAG 
GGCGAGGTGC CCACGGCCAT GCAGCAGACT ACAAGCCTGG CTCAGGCAAA GCCCTTGGAG 
AAAAACTCTC AGGTCAGAGC TGCCTCAGCC ACAGGTGCAG GACCCTCGGC AAAGGGCACG 
GTCCCAGCGC TCCCTCAGAA GGCAAGGTCT GTGGCCGCCC AGGATGGGAA GCAGGAGGAC 
TCAGAGAGCA GCAGTGAGGA GGAGTCAGAC AGCGAGGAGG AGATGCAGAA GGCAGGGACC 
TCAGCCCAGG CACAGTCCTC TGGGAAAGCC CTCCAGATCA GACCTGCCTC CGGTCCCACC 
AAGAGGCCCC CTCAGAAGGC CGGGCCTGCA ACCACCCAGG TCAAGGTTGA AAGGGCCGAG 
GAAGACTCGG AGAGCAGTGA GGAGGAATCA GACAGTGAGG ATGAAGCTCC TGTAGCCAAG 
ACTCCAGCCC AGGCAAAATC AGCTGTGAAA ACTTCTCAGA TCAAGGCCTC CCCAAGGAAG 
GGCACCCCCA TTACACCCTC ATCTGCCAGG GTCCCCCCAG TGCAAGTCGG CACACCAGCC 
CCCCGGACAG CAGCAGCAGT GAGTACGCCC ACCTGCGCGT CATCCCCAGC CATAGTCAGG 
CACACCCAGA AGCCAGGGGA GGACTCTTCA AGCAGCGAGG AGTCCGAGAG CGAGGAGGAG 
ACAGCTCCTG CTGCAGCAGG GGGACAGATG AAGTCTATAG GGAAGACCCT CCCAGTGAAA 
GCTGCCTCAC CAGCCACCAA GGGGCCCTCA GGAAAAGGGA CCACCCCAGG GCCCCCTGGG 
AAGGCAGGAC CTGCAGTGGC ACAGGTCAAG GCTGAGGTGC AGGAAGACTC AGAAAGCAGT 
GAGGAGTCGG ACAATGAGGA AGCGGCTGCA ACTCCCGCAC AGGTGAAGAC TTCAGTGAAA 
ACCCCTCAGA CCAAAGCCAA CTCTTCTGCC ACCAGAGTGG CTTCAGCCAA AGCAGCAGCA 
CCAGCTCCTG GAAAGGGGGG CCTTGCTGTT GCTCAAGCCA AAGTGGGGTC CCCAGCCAAG 
GTAAAACCAC CAGCGAGAAC CCCCCAGAGT AGCGCCGTCT CTGGGAGGGG CCAGGTGTCT 
GTGCCGGCTG TGGGGAAGGC AGCAGCTGCA GCAGCCCAGG CCCAGCCGGG GCCTGTCAAG 
GGCTCACAGG AGGACTCGGA GAGCAGTGAG GAGGAGTCGG ACAGTGAGGG AGAGGCACCT 
CCTCAGGGGA AGCCCTCAGG AAAGACCCCC CAGGTCAGAA CTGCCTCAGC CCCCACCAAG 
GCGTCCCCCA AGAAAGGGGC TGCCCCAGTA CCCCCTGGGA AAGCAGGACC CCCAGCCACC 
CAGGCCAAGA GGCAGGAGGA GGACTCGGAG AGCAGCAGCG AGGAGGAGTC GGACAGTGAC 
GGGGACGTGC CAGCTGCTGC ACCTCCAGCC CAGAAAGAGG GTAGCTCCAA AACTGCCACA 
AGCAAGACCC TGGCCCCAGC GTCCCTGGAG AAGAAAGCAG AGGAGTCCTC AACCAGCAGT 
GATGAGGACC TGCCATCCAG CCAGGTGACC AAGAGGAACC TCCCGCTGAC ACAGGCTGCC 
CTGAAGGTTC TTGCCCAGAA AGCCAGTGAG GCCCAATCTC CTGCTGCCAA GACCCCGTCT 
TCAAGTGGGA CTGACAGTGC TGTGGGAACA CTCTCCACGG CAAGTCCCCA GAGCACCCCT 
ACCCAGGTCA GAATGGCCAA CAAGCTCAGA AAGCCTGAGG TTCCTACAGC TCAGCAGGCC 
ACAGCCACTC CCTCGGGACA CCCCACAGCC AAGGCCCCTG CAACCTCAGA TGACAGCAAC 
AGCAGCTCTT CAGGGAGCGA GGACGATGCC AAGGGGTCCC AGGCGGCCCC GTCAGCCCAC 
AGGCCAGGCC CAGCCCCCTC CAGGAGGGAG ACCTTGGTGG AAGAGACCAC AACAGAGTCC 
AGTGACGACG AGGTGGTGGC ACCTTCCCAG TCTCTCCTCT CAGGTTTTGT GACTCCTGGG 
CTGACTCCAG CTGGTTCCAA GACTTCAAAG GCCGCTCCCA AACCAGACGC CAGCCCCTCT 
GTTTCCTCTA CTCCGGCCAC CAGAGATGCC CCAGAGGGCA AGCAGGAAGT GGAGCCCCAA 
CAAGCAGCAG GCACCATGTC CCCTAAAACA GGCAGAAAAG AGGCTGGTGC CACACCTCAG 
AAGCCCGGGA AGGGGCTCGG GAGTCCCCCG GCCTCCATGC TGGCGCTGCA GAGCAACATC 
ACCCAGCGCC TCCTGAGCGA GCCCTGGCCC CTGAGCGAGG CGCAGGTGCA GGCCTCGGTG 
GCGAAGGTGC TCACGGAGCT GCTGGAGCAG GAGAGGAAGA AGGCCATGGA CACCGCCAAG 
GAAGGCAGCC GGAAGGGCCG CCTGGGCCAT AAGCGGAAGC TGTCCGAAGA CCAGACAGCC 
CCCAAGGCCC CCAAGAACAA GAAAAAGAAG CAGCTGGCAG CTGCAGATGG TGGGGAGCAT 
GTCGTCTCCT CAGAAAAGGC CCCCAGGACT GTCAAAGGGA AATCCAAGAA GGACAGAGCA 
AGTGGTGACG TCAAGGAGAA GAAGGAGAAG GAGTCTCCCG GCTCTCAAGG GGCCAAGGAG 
AAGCCAGTAG GGGAGCTGGG GACTCCAAAG GGTGACGGGG GAGACCACGG CAACCTTAAG 
ATGAAGAAAG AGAAGAAGAA ATCTGACAAG AAAAAAAAAG ACAAGGAGAA AAAGGAAAAG 
AAGAAGAAAG CAAAAAAGGC CTCAACCAAA GACCCTGGCT CACCATCACA GAAGAAAAAG 
AAGAGAAAGA AGAAGACAGC AGAGCAAACC GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005209691.1
CDS	77..4132
Translation	MAEARKRRELLPLIYQHLLQAGYVRAAREVKEQSGQKIFLSQPVTLLDIYTHWQQTSEIGRKRKAEEDAALQAKRSRVSDPISSSESSEEEEEEEEAEAKTTKPTPRLAATNSSVTGTVSSSNVKEKAKAKTTKASKTVNSTTHPAPGKAVAHLLTGKSPQKTSGPSANAVLVSETEEEGSVSALRTATTPGMASADQADSSSEDTTSSSDETDLEAKAAVKPLQVRASATAVKESPRKSAAPTPGKAGVVTPQVRGGAVTPASRAKKPEEVSESSEESDESEEAAPAGTPSQVKALEKTVPIRVAPSPAKGTPGKGATPAPPGKAGPLGKPEEDSESSSEESDSEEETQAVKTPVQAKPSGKIPQVKAASTPKIQVFFPKKGSPPTSPGKAGPAATQAQPRKQEEEESSSSSSKESDSEGEVPTAMQQTTSLAQAKPLEKNSQVRAASATGAGPSAKGTVPALPQKARSVAAQDGKQEDSESSSEEESDSEEEMQKAGTSAQAQSSGKALQIRPASGPTKRPPQKAGPATTQVKVERAEEDSESSEEESDSEDEAPVAKTPAQAKSAVKTSQIKASPRKGTPITPSSARVPPVQVGTPAPRTAAAVSTPTCASSPAIVRHTQKPGEDSSSSEESESEEETAPAAAGGQMKSIGKTLPVKAASPATKGPSGKGTTPGPPGKAGPAVAQVKAEVQEDSESSEESDNEEAAATPAQVKTSVKTPQTKANSSATRVASAKAAAPAPGKGGLAVAQAKVGSPAKVKPPARTPQSSAVSGRGQVSVPAVGKAAAAAAQAQPGPVKGSQEDSESSEEESDSEGEAPPQGKPSGKTPQVRTASAPTKASPKKGAAPVPPGKAGPPATQAKRQEEDSESSSEEESDSDGDVPAAAPPAQKEGSSKTATSKTLAPASLEKKAEESSTSSDEDLPSSQVTKRNLPLTQAALKVLAQKASEAQSPAAKTPSSSGTDSAVGTLSTASPQSTPTQVRMANKLRKPEVPTAQQATATPSGHPTAKAPATSDDSNSSSSGSEDDAKGSQAAPSAHRPGPAPSRRETLVEETTTESSDDEVVAPSQSLLSGFVTPGLTPAGSKTSKAAPKPDASPSVSSTPATRDAPEGKQEVEPQQAAGTMSPKTGRKEAGATPQKPGKGLGSPPASMLALQSNITQRLLSEPWPLSEAQVQASVAKVLTELLEQERKKAMDTAKEGSRKGRLGHKRKLSEDQTAPKAPKNKKKKQLAAADGGEHVVSSEKAPRTVKGKSKKDRASGDVKEKKEKESPGSQGAKEKPVGELGTPKGDGGDHGNLKMKKEKKKSDKKKKDKEKKEKKKKAKKASTKDPGSPSQKKKKRKKKTAEQTV

Target ORF information:

RefSeq Version	XM_005209634.4
Organism	Bos taurus(cattle)
Definition	Bos taurus treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005209634.4

ORF Insert Sequence:

ATGGCTGAGG CCAGGAAGCG GCGAGAGCTC CTTCCCCTGA TCTACCAGCA TCTGCTGCAG 
GCGGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA AATTTTCCTG 
AGTCAGCCTG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACTTC GGAGATTGGC 
CGGAAACGGA AGGCAGAGGA AGATGCGGCC TTGCAGGCCA AGAGGAGCCG TGTGTCAGAC 
CCCATCAGCA GCTCAGAAAG CTCAGAAGAG GAGGAGGAAG AGGAGGAGGC AGAAGCCAAA 
ACCACCAAAC CCACCCCGAG ATTAGCAGCT ACCAACTCCT CAGTCACAGG GACAGTTTCG 
TCTTCAAATG TGAAAGAAAA AGCCAAGGCA AAGACCACGA AAGCCAGCAA GACGGTGAAC 
TCCACGACAC ACCCTGCCCC GGGAAAGGCA GTGGCCCACC TCCTCACTGG GAAGTCACCC 
CAGAAGACAT CGGGGCCCTC GGCAAATGCT GTCTTGGTGT CAGAAACCGA GGAGGAGGGC 
AGCGTGTCAG CCCTCAGAAC CGCCACCACG CCTGGAATGG CATCCGCCGA CCAGGCTGAC 
AGCTCCAGTG AGGATACCAC CAGCTCGAGT GATGAGACGG ACTTGGAGGC GAAAGCCGCA 
GTAAAACCAC TGCAGGTCAG AGCCTCAGCC ACTGCAGTCA AGGAGTCTCC GAGAAAAAGT 
GCAGCCCCGA CTCCAGGGAA GGCAGGGGTT GTGACACCCC AAGTCAGAGG CGGTGCTGTG 
ACCCCAGCCA GCAGGGCCAA GAAGCCAGAA GAGGTCTCAG AAAGCAGTGA AGAGTCCGAC 
GAGAGTGAGG AGGCCGCCCC TGCGGGGACA CCCAGTCAGG TGAAGGCCTT GGAGAAAACC 
GTCCCAATCA GAGTTGCTCC TAGTCCTGCC AAGGGGACCC CTGGGAAAGG GGCCACCCCA 
GCACCCCCTG GGAAGGCAGG GCCCTTAGGG AAGCCAGAGG AGGACTCGGA GAGCAGCAGC 
GAGGAGTCAG ACAGCGAGGA GGAGACACAA GCTGTCAAGA CCCCAGTGCA GGCAAAGCCC 
TCTGGGAAGA TCCCCCAGGT TAAAGCTGCC TCCACACCAA AAATCCAGGT GTTTTTCCCT 
AAGAAAGGGT CCCCTCCAAC ATCCCCTGGC AAGGCAGGGC CTGCAGCCAC CCAAGCCCAG 
CCCAGGAAGC AGGAGGAGGA GGAGAGCAGC AGCAGCAGCA GCAAGGAATC AGACAGCGAG 
GGCGAGGTGC CCACGGCCAT GCAGCAGACT ACAAGCCTGG CTCAGGCAAA GCCCTTGGAG 
AAAAACTCTC AGGTCAGAGC TGCCTCAGCC ACAGGTGCAG GACCCTCGGC AAAGGGCACG 
GTCCCAGCGC TCCCTCAGAA GGCAAGGTCT GTGGCCGCCC AGGATGGGAA GCAGGAGGAC 
TCAGAGAGCA GCAGTGAGGA GGAGTCAGAC AGCGAGGAGG AGATGCAGAA GGCAGGGACC 
TCAGCCCAGG CACAGTCCTC TGGGAAAGCC CTCCAGATCA GACCTGCCTC CGGTCCCACC 
AAGAGGCCCC CTCAGAAGGC CGGGCCTGCA ACCACCCAGG TCAAGGTTGA AAGGGCCGAG 
GAAGACTCGG AGAGCAGTGA GGAGGAATCA GACAGTGAGG ATGAAGCTCC TGTAGCCAAG 
ACTCCAGCCC AGGCAAAATC AGCTGTGAAA ACTTCTCAGA TCAAGGCCTC CCCAAGGAAG 
GGCACCCCCA TTACACCCTC ATCTGCCAGG GTCCCCCCAG TGCAAGTCGG CACACCAGCC 
CCCCGGACAG CAGCAGCAGT GAGTACGCCC ACCTGCGCGT CATCCCCAGC CATAGTCAGG 
CACACCCAGA AGCCAGGGGA GGACTCTTCA AGCAGCGAGG AGTCCGAGAG CGAGGAGGAG 
ACAGCTCCTG CTGCAGCAGG GGGACAGATG AAGTCTATAG GGAAGACCCT CCCAGTGAAA 
GCTGCCTCAC CAGCCACCAA GGGGCCCTCA GGAAAAGGGA CCACCCCAGG GCCCCCTGGG 
AAGGCAGGAC CTGCAGTGGC ACAGGTCAAG GCTGAGGTGC AGGAAGACTC AGAAAGCAGT 
GAGGAGTCGG ACAATGAGGA AGCGGCTGCA ACTCCCGCAC AGGTGAAGAC TTCAGTGAAA 
ACCCCTCAGA CCAAAGCCAA CTCTTCTGCC ACCAGAGTGG CTTCAGCCAA AGCAGCAGCA 
CCAGCTCCTG GAAAGGGGGG CCTTGCTGTT GCTCAAGCCA AAGTGGGGTC CCCAGCCAAG 
GTAAAACCAC CAGCGAGAAC CCCCCAGAGT AGCGCCGTCT CTGGGAGGGG CCAGGTGTCT 
GTGCCGGCTG TGGGGAAGGC AGCAGCTGCA GCAGCCCAGG CCCAGCCGGG GCCTGTCAAG 
GGCTCACAGG AGGACTCGGA GAGCAGTGAG GAGGAGTCGG ACAGTGAGGG AGAGGCACCT 
CCTCAGGGGA AGCCCTCAGG AAAGACCCCC CAGGTCAGAA CTGCCTCAGC CCCCACCAAG 
GCGTCCCCCA AGAAAGGGGC TGCCCCAGTA CCCCCTGGGA AAGCAGGACC CCCAGCCACC 
CAGGCCAAGA GGCAGGAGGA GGACTCGGAG AGCAGCAGCG AGGAGGAGTC GGACAGTGAC 
GGGGACGTGC CAGCTGCTGC ACCTCCAGCC CAGAAAGAGG GTAGCTCCAA AACTGCCACA 
AGCAAGACCC TGGCCCCAGC GTCCCTGGAG AAGAAAGCAG AGGAGTCCTC AACCAGCAGT 
GATGAGGACC TGCCATCCAG CCAGGTGACC AAGAGGAACC TCCCGCTGAC ACAGGCTGCC 
CTGAAGGTTC TTGCCCAGAA AGCCAGTGAG GCCCAATCTC CTGCTGCCAA GACCCCGTCT 
TCAAGTGGGA CTGACAGTGC TGTGGGAACA CTCTCCACGG CAAGTCCCCA GAGCACCCCT 
ACCCAGGTCA GAATGGCCAA CAAGCTCAGA AAGCCTGAGG TTCCTACAGC TCAGCAGGCC 
ACAGCCACTC CCTCGGGACA CCCCACAGCC AAGGCCCCTG CAACCTCAGA TGACAGCAAC 
AGCAGCTCTT CAGGGAGCGA GGACGATGCC AAGGGGTCCC AGGCGGCCCC GTCAGCCCAC 
AGGCCAGGCC CAGCCCCCTC CAGGAGGGAG ACCTTGGTGG AAGAGACCAC AACAGAGTCC 
AGTGACGACG AGGTGGTGGC ACCTTCCCAG TCTCTCCTCT CAGGTTTTGT GACTCCTGGG 
CTGACTCCAG CTGGTTCCAA GACTTCAAAG GCCGCTCCCA AACCAGACGC CAGCCCCTCT 
GTTTCCTCTA CTCCGGCCAC CAGAGATGCC CCAGAGGGCA AGCAGGAAGT GGAGCCCCAA 
CAAGCAGCAG GCACCATGTC CCCTAAAACA GGCAGAAAAG AGGCTGGTGC CACACCTCAG 
AAGCCCGGGA AGGGGCTCGG GAGTCCCCCG GCCTCCATGC TGGCGCTGCA GAGCAACATC 
ACCCAGCGCC TCCTGAGCGA GCCCTGGCCC CTGAGCGAGG CGCAGGTGCA GGCCTCGGTG 
GCGAAGGTGC TCACGGAGCT GCTGGAGCAG GAGAGGAAGA AGGCCATGGA CACCGCCAAG 
GAAGGCAGCC GGAAGGGCCG CCTGGGCCAT AAGCGGAAGC TGTCCGAAGA CCAGACAGCC 
CCCAAGGCCC CCAAGAACAA GAAAAAGAAG CAGCTGGCAG CTGCAGATGG TGGGGAGCAT 
GTCGTCTCCT CAGAAAAGGC CCCCAGGACT GTCAAAGGGA AATCCAAGAA GGACAGAGCA 
AGTGGTGACG TCAAGGAGAA GAAGGAGAAG GAGTCTCCCG GCTCTCAAGG GGCCAAGGAG 
AAGCCAGTAG GGGAGCTGGG GACTCCAAAG GGTGACGGGG GAGACCACGG CAACCTTAAG 
ATGAAGAAAG AGAAGAAGAA ATCTGACAAG AAAAAAAAAG ACAAGGAGAA AAAGGAAAAG 
AAGAAGAAAG CAAAAAAGGC CTCAACCAAA GACCCTGGCT CACCATCACA GAAGAAAAAG 
AAGAGAAAGA AGAAGACAGC AGAGCAAACC GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_002689293.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002689339.2 Bos taurus Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant X1, mRNA.

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CloneID	OBa05568
Clone ID Related Accession (Same CDS sequence)	XM_002689293.5 , XM_010807517.2 , XM_591291.9 , XM_010798510.2
Accession Version	XM_002689293.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4377bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	treacle protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104039.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002689293.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAGG CCAGGAAGCG GCGAGAGCTC CTTCCCCTGA TCTACCAGCA TCTGCTGCAG 
GCGGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA AATTTTCCTG 
AGTCAGCCTG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACTTC GGAGATTGGC 
CGGAAACGGA AGGCAGAGGA AGATGCGGCC TTGCAGGCCA AGAGGAGCCG TGTGTCAGAC 
CCCATCAGCA GCTCAGAAAG CTCAGAAGAG GAGGAGGAAG AGGAGGAGGC AGAAGCCAAA 
ACCACCAAAC CCACCCCGAG ATTAGCAGCT ACCAACTCCT CAGTCACAGG GACAGTTTCG 
TCTTCAAATG TGAAAGAAAA AGCCAAGGCA AAGACCACGA AAGCCAGCAA GACGGTGAAC 
TCCACGACAC ACCCTGCCCC GGGAAAGGCA GTGGCCCACC TCCTCACTGG GAAGTCACCC 
CAGAAGACAT CGGGGCCCTC GGCAAATGCT GTCTTGGTGT CAGAAACCGA GGAGGAGGGC 
AGCGTGTCAG CCCTCAGAAC CGCCACCACG CCTGGAATGG CATCCGCCGA CCAGGCTGAC 
AGCTCCAGTG AGGATACCAC CAGCTCGAGT GATGAGACGG ACTTGGAGGC GAAAGCCGCA 
GTAAAACCAC TGCAGGTCAG AGCCTCAGCC ACTGCAGTCA AGGAGTCTCC GAGAAAAAGT 
GCAGCCCCGA CTCCAGGGAA GGCAGGGGTT GTGACACCCC AAGTCAGAGG CGGTGCTGTG 
ACCCCAGCCA GCAGGGCCAA GAAGCCAGAA GAGGTCTCAG AGAGCAGTGA AGAGTCCGAC 
GAGAGTGAGG AGGCCGCCCC TGCGGGGACA CCCAGTCAGG TGAAGGCCTT GGAGAAAACC 
GTCCCAATCA GAGTTGCTCC TAGTCCTGCC AAGGGGACCC CTGGGAAAGG GGCCACCCCA 
GCACCCCCTG GGAAGGCAGG GCCCTTAGGG AAGCCAGAGG AGGACTCGGA GAGCAGCAGC 
GAGGAGTCAG ACAGCGAGGA GGAGACACAA GCTGTCAAGA CCCCAGTGCA GGCAAAGCCC 
TCTGGGAAGA TCCCCCAGGT TAAAGCTGCC TCCACACCAA AAATCCAGGT GTTTTTCCCT 
AAGAAAGGGT CCCCTCCAAC ATCCCCTGGC AAGGCAGGGC CTGCAGCCAC CCAAGCCCAG 
CCCAGGAAGC AGGAGGAGGA GGAGAGCAGC AGCAGCAGCA GCAAGGAATC AGACAGCGAG 
GGCGAGGTGC CCACGGCCAT GCAGCAGACT ACAAGCCTGG CTCAGGCAAA GCCCTTGGAG 
AAAAACTCTC AGGTCAGAGC TGCCTCAGCC ACAGGTGCAG GACCCTCGGC AAAGGGCACG 
GTCCCAGCGC TCCCTCAGAA GGCAAGGTCT GTGGCCGCCC AGGATGGGAA GCAGGAGGAC 
TCAGAGAGCA GCAGTGAGGA GGAGTCAGAC AGCGAGGAGG AGATGCAGAA GGCAGGGACC 
TCAGCCCAGG CACAGTCCTC TGGGAAAGCC CTCCAGATCA GACCTGCCTC CGGTCCCACC 
AAGAGGCCCC CTCAGAAGGC CGGGCCTGCA ACCACCCAGG TCAAGGTTGA AAGGGCCGAG 
GAAGACTCGG AGAGCAGTGA GGAGGAATCA GACAGTGAGG ATGAAGCTCC TGTAGCCAAG 
ACTCCAGCCC AGGCAAAATC AGCTGTGAAA ACTTCTCAGA TCAAGGCCTC CCCAAGGAAG 
GGCACCCCCA TTACACCCTC ATCTGCCAGG GTCCCCCCAG TGCAAGTCGG CACACCAGCC 
CCCCGGACAG CAGCAGCAGT GAGTACGCCC ACCTGCGCGT CATCCCCAGC CATAGTCAGG 
CACACCCAGA AGCCAGGGGA GGACTCTTCA AGCAGCGAGG AGTCCGAGAG CGAGGAGGAG 
ACAGCTCCTG CTGCAGCAGG GGGACAGATG AAGTCTATAG GGAAGACCCT CCCAGTGAAA 
GCTGCCTCAC CAGCCACCAA GGGGCCCTCA GGAAAAGGGA CCACCCCAGG GCCCCCTGGG 
AAGGCAGGAC CTGCAGTGGC ACAGGTCAAG GCTGAGGTGC AGGAAGACTC AGAAAGCAGT 
GAGGAGTCGG ACAATGAGGA AGCGGCTGCA ACTCCCGCAC AGGTGAAGAC TTCAGTGAAA 
ACCCCTCAGA CCAAAGCCAA CTCTTCTGCC ACCAGAGTGG CTTCAGCCAA AGCAGCAGCA 
CCAGCTCCTG GAAAGGGGGG CCTTGCTGTT GCTCAAGCCA AAGTGGGGTC CCCAGCCAAG 
GTAAAACCAC CAGCGAGAAC CCCCCAGAGT AGCGCCGTCT CTGGGAGGGG CCAGGTGTCT 
GTGCCGGCTG TGGGGAAGGC AGCAGCTGCA GCAGCCCAGG CCCAGCCGGG GCCTGTCAAG 
GGCTCACAGG AGGACTCGGA GAGCAGTGAG GAGGAGTCGG ACAGTGAGGG AGAGGCACCT 
CCTCAGGGGA AGCCCTCAGG AAAGACCCCC CAGGTCAGAA CTGCCTCAGC CCCCACCAAG 
GCGTCCCCCA AGAAAGGGGC TGCCCCAGTA CCCCCTGGGA AAGCAGGACC CCCAGCCACC 
CAGGCCAAGA GGCAGGAGGA GGACTCGGAG AGCAGCAGCG AGGAGGAGTC GGACAGTGAC 
GGGGACGTGC CAGCTGCTGC ACCTCCAGCC CAGAAAGAGG GTAGCTCCAA AACTGCCACA 
AGCAAGACCC TGGCCCCAGC GTCCCTGGAG AAGAAAGCAG AGGAGTCCTC AACCAGCAGT 
GATGAGGACC TGCCATCCAG CCAGGTGATT AAAACCCCTC TGATTTTTGT CGACCCTAAT 
CGTAGTCCAG CTGGTCCAGC TGCTACCCCC GCCCAAGCGC AGGCTACAGG CACCCTGAGG 
AAGGCTCAGG CCTTGGAGAG CACAGCCAGG AGCTCCTCTG AGAGCGAGGA CGAGGACGTG 
ATCCCCGCTA CACAGTGCCC GACTCCTGCC ACTAGGACCA GCGTGGTGAC TGTGCCCACG 
GCACCCCCAA AGGCGGCCCT CAGAGCCAGT GTGGTGGGGG CTCCCAGCAG TGAGGACACA 
AGTCAGGGGT CTGAAGGCAA GAAGCAGGAG GCCTCAGCCA CTCAGGTGAC CAAGAGGAAC 
CTCCCGCTGA CACAGGCTGC CCTGAAGGTT CTTGCCCAGA AAGCCAGTGA GGCCCAATCT 
CCTGCTGCCA AGACCCCGTC TTCAAGTGGG ACTGACAGTG CTGTGGGAAC ACTCTCCACG 
GCAAGTCCCC AGAGCACCCC TACCCAGGTC AGAATGGCCA ACAAGCTCAG AAAGCCTGAG 
GTTCCTACAG CTCAGCAGGC CACAGCCACT CCCTCGGGAC ACCCCACAGC CAAGGCCCCT 
GCAACCTCAG ATGACAGCAA CAGCAGCTCT TCAGGGAGCG AGGACGATGC CAAGGGGTCC 
CAGGCGGCCC CGTCAGCCCA CAGGCCAGGC CCAGCCCCCT CCAGGAGGGA GACCTTGGTG 
GAAGAGACCA CAACAGAGTC CAGTGACGAC GAGGTGGTGG CACCTTCCCA GTCTCTCCTC 
TCAGGTTTTG TGACTCCTGG GCTGACTCCA GCTGGTTCCA AGACTTCAAA GGCCGCTCCC 
AAACCAGACG CCAGCCCCTC TGTTTCCTCT ACTCCGGCCA CCAGAGATGC CCCAGAGGGC 
AAGCAGGAAG TGGAGCCCCA ACAAGCAGCA GGCACCATGT CCCCTAAAAC AGGCAGAAAA 
GAGGCTGGTG CCACACCTCA GAAGCCCGGG AAGGGGCTCG GGAGTCCCCC GGCCTCCATG 
CTGGCGCTGC AGAGCAACAT CACCCAGCGC CTCCTGAGCG AGCCCTGGCC CCTGAGCGAG 
GCGCAGGTGC AGGCCTCGGT GGCGAAGGTG CTCACGGAGC TGCTGGAGCA GGAGAGGAAG 
AAGGCCATGG ACACCGCCAA GGAAGGCAGC CGGAAGGGCC GCCTGGGCCA TAAGCGGAAG 
CTGTCCGAAG ACCAGACAGC CCCCAAGGCC CCCAAGAACA AGAAAAAGAA GCAGCTGGCA 
GCTGCAGATG GTGGGGAGCA TGTCGTCTCC TCAGAAAAGG CCCCCAGGAC TGTCAAAGGG 
AAATCCAAGA AGGACAGAGC AAGTGGTGAC GTCAAGGAGA AGAAGGAGAA GGAGTCTCCC 
GGCTCTCAAG GGGCCAAGGA GAAGCCAGTA GGGGAGCTGG GGACTCCAAA GGGTGACGGG 
GGAGACCACG GCAACCTTAA GATGAAGAAA GAGAAGAAGA AATCTGACAA GAAAAAAAAA 
GACAAGGAGA AAAAGGAAAA GAAGAAGAAA GCAAAAAAGG CCTCAACCAA AGACCCTGGC 
TCACCATCAC AGAAGAAAAA GAAGAGAAAG AAGAAGACAG CAGAGCAAAC CGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002689339.2
CDS	59..4435
Translation	MAEARKRRELLPLIYQHLLQAGYVRAAREVKEQSGQKIFLSQPVTLLDIYTHWQQTSEIGRKRKAEEDAALQAKRSRVSDPISSSESSEEEEEEEEAEAKTTKPTPRLAATNSSVTGTVSSSNVKEKAKAKTTKASKTVNSTTHPAPGKAVAHLLTGKSPQKTSGPSANAVLVSETEEEGSVSALRTATTPGMASADQADSSSEDTTSSSDETDLEAKAAVKPLQVRASATAVKESPRKSAAPTPGKAGVVTPQVRGGAVTPASRAKKPEEVSESSEESDESEEAAPAGTPSQVKALEKTVPIRVAPSPAKGTPGKGATPAPPGKAGPLGKPEEDSESSSEESDSEEETQAVKTPVQAKPSGKIPQVKAASTPKIQVFFPKKGSPPTSPGKAGPAATQAQPRKQEEEESSSSSSKESDSEGEVPTAMQQTTSLAQAKPLEKNSQVRAASATGAGPSAKGTVPALPQKARSVAAQDGKQEDSESSSEEESDSEEEMQKAGTSAQAQSSGKALQIRPASGPTKRPPQKAGPATTQVKVERAEEDSESSEEESDSEDEAPVAKTPAQAKSAVKTSQIKASPRKGTPITPSSARVPPVQVGTPAPRTAAAVSTPTCASSPAIVRHTQKPGEDSSSSEESESEEETAPAAAGGQMKSIGKTLPVKAASPATKGPSGKGTTPGPPGKAGPAVAQVKAEVQEDSESSEESDNEEAAATPAQVKTSVKTPQTKANSSATRVASAKAAAPAPGKGGLAVAQAKVGSPAKVKPPARTPQSSAVSGRGQVSVPAVGKAAAAAAQAQPGPVKGSQEDSESSEEESDSEGEAPPQGKPSGKTPQVRTASAPTKASPKKGAAPVPPGKAGPPATQAKRQEEDSESSSEEESDSDGDVPAAAPPAQKEGSSKTATSKTLAPASLEKKAEESSTSSDEDLPSSQVIKTPLIFVDPNRSPAGPAATPAQAQATGTLRKAQALESTARSSSESEDEDVIPATQCPTPATRTSVVTVPTAPPKAALRASVVGAPSSEDTSQGSEGKKQEASATQVTKRNLPLTQAALKVLAQKASEAQSPAAKTPSSSGTDSAVGTLSTASPQSTPTQVRMANKLRKPEVPTAQQATATPSGHPTAKAPATSDDSNSSSSGSEDDAKGSQAAPSAHRPGPAPSRRETLVEETTTESSDDEVVAPSQSLLSGFVTPGLTPAGSKTSKAAPKPDASPSVSSTPATRDAPEGKQEVEPQQAAGTMSPKTGRKEAGATPQKPGKGLGSPPASMLALQSNITQRLLSEPWPLSEAQVQASVAKVLTELLEQERKKAMDTAKEGSRKGRLGHKRKLSEDQTAPKAPKNKKKKQLAAADGGEHVVSSEKAPRTVKGKSKKDRASGDVKEKKEKESPGSQGAKEKPVGELGTPKGDGGDHGNLKMKKEKKKSDKKKKDKEKKEKKKKAKKASTKDPGSPSQKKKKRKKKTAEQTV

Target ORF information:

RefSeq Version	XM_002689293.5
Organism	Bos taurus(cattle)
Definition	Bos taurus Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002689293.5

ORF Insert Sequence:

ATGGCTGAGG CCAGGAAGCG GCGAGAGCTC CTTCCCCTGA TCTACCAGCA TCTGCTGCAG 
GCGGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA AATTTTCCTG 
AGTCAGCCTG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACTTC GGAGATTGGC 
CGGAAACGGA AGGCAGAGGA AGATGCGGCC TTGCAGGCCA AGAGGAGCCG TGTGTCAGAC 
CCCATCAGCA GCTCAGAAAG CTCAGAAGAG GAGGAGGAAG AGGAGGAGGC AGAAGCCAAA 
ACCACCAAAC CCACCCCGAG ATTAGCAGCT ACCAACTCCT CAGTCACAGG GACAGTTTCG 
TCTTCAAATG TGAAAGAAAA AGCCAAGGCA AAGACCACGA AAGCCAGCAA GACGGTGAAC 
TCCACGACAC ACCCTGCCCC GGGAAAGGCA GTGGCCCACC TCCTCACTGG GAAGTCACCC 
CAGAAGACAT CGGGGCCCTC GGCAAATGCT GTCTTGGTGT CAGAAACCGA GGAGGAGGGC 
AGCGTGTCAG CCCTCAGAAC CGCCACCACG CCTGGAATGG CATCCGCCGA CCAGGCTGAC 
AGCTCCAGTG AGGATACCAC CAGCTCGAGT GATGAGACGG ACTTGGAGGC GAAAGCCGCA 
GTAAAACCAC TGCAGGTCAG AGCCTCAGCC ACTGCAGTCA AGGAGTCTCC GAGAAAAAGT 
GCAGCCCCGA CTCCAGGGAA GGCAGGGGTT GTGACACCCC AAGTCAGAGG CGGTGCTGTG 
ACCCCAGCCA GCAGGGCCAA GAAGCCAGAA GAGGTCTCAG AGAGCAGTGA AGAGTCCGAC 
GAGAGTGAGG AGGCCGCCCC TGCGGGGACA CCCAGTCAGG TGAAGGCCTT GGAGAAAACC 
GTCCCAATCA GAGTTGCTCC TAGTCCTGCC AAGGGGACCC CTGGGAAAGG GGCCACCCCA 
GCACCCCCTG GGAAGGCAGG GCCCTTAGGG AAGCCAGAGG AGGACTCGGA GAGCAGCAGC 
GAGGAGTCAG ACAGCGAGGA GGAGACACAA GCTGTCAAGA CCCCAGTGCA GGCAAAGCCC 
TCTGGGAAGA TCCCCCAGGT TAAAGCTGCC TCCACACCAA AAATCCAGGT GTTTTTCCCT 
AAGAAAGGGT CCCCTCCAAC ATCCCCTGGC AAGGCAGGGC CTGCAGCCAC CCAAGCCCAG 
CCCAGGAAGC AGGAGGAGGA GGAGAGCAGC AGCAGCAGCA GCAAGGAATC AGACAGCGAG 
GGCGAGGTGC CCACGGCCAT GCAGCAGACT ACAAGCCTGG CTCAGGCAAA GCCCTTGGAG 
AAAAACTCTC AGGTCAGAGC TGCCTCAGCC ACAGGTGCAG GACCCTCGGC AAAGGGCACG 
GTCCCAGCGC TCCCTCAGAA GGCAAGGTCT GTGGCCGCCC AGGATGGGAA GCAGGAGGAC 
TCAGAGAGCA GCAGTGAGGA GGAGTCAGAC AGCGAGGAGG AGATGCAGAA GGCAGGGACC 
TCAGCCCAGG CACAGTCCTC TGGGAAAGCC CTCCAGATCA GACCTGCCTC CGGTCCCACC 
AAGAGGCCCC CTCAGAAGGC CGGGCCTGCA ACCACCCAGG TCAAGGTTGA AAGGGCCGAG 
GAAGACTCGG AGAGCAGTGA GGAGGAATCA GACAGTGAGG ATGAAGCTCC TGTAGCCAAG 
ACTCCAGCCC AGGCAAAATC AGCTGTGAAA ACTTCTCAGA TCAAGGCCTC CCCAAGGAAG 
GGCACCCCCA TTACACCCTC ATCTGCCAGG GTCCCCCCAG TGCAAGTCGG CACACCAGCC 
CCCCGGACAG CAGCAGCAGT GAGTACGCCC ACCTGCGCGT CATCCCCAGC CATAGTCAGG 
CACACCCAGA AGCCAGGGGA GGACTCTTCA AGCAGCGAGG AGTCCGAGAG CGAGGAGGAG 
ACAGCTCCTG CTGCAGCAGG GGGACAGATG AAGTCTATAG GGAAGACCCT CCCAGTGAAA 
GCTGCCTCAC CAGCCACCAA GGGGCCCTCA GGAAAAGGGA CCACCCCAGG GCCCCCTGGG 
AAGGCAGGAC CTGCAGTGGC ACAGGTCAAG GCTGAGGTGC AGGAAGACTC AGAAAGCAGT 
GAGGAGTCGG ACAATGAGGA AGCGGCTGCA ACTCCCGCAC AGGTGAAGAC TTCAGTGAAA 
ACCCCTCAGA CCAAAGCCAA CTCTTCTGCC ACCAGAGTGG CTTCAGCCAA AGCAGCAGCA 
CCAGCTCCTG GAAAGGGGGG CCTTGCTGTT GCTCAAGCCA AAGTGGGGTC CCCAGCCAAG 
GTAAAACCAC CAGCGAGAAC CCCCCAGAGT AGCGCCGTCT CTGGGAGGGG CCAGGTGTCT 
GTGCCGGCTG TGGGGAAGGC AGCAGCTGCA GCAGCCCAGG CCCAGCCGGG GCCTGTCAAG 
GGCTCACAGG AGGACTCGGA GAGCAGTGAG GAGGAGTCGG ACAGTGAGGG AGAGGCACCT 
CCTCAGGGGA AGCCCTCAGG AAAGACCCCC CAGGTCAGAA CTGCCTCAGC CCCCACCAAG 
GCGTCCCCCA AGAAAGGGGC TGCCCCAGTA CCCCCTGGGA AAGCAGGACC CCCAGCCACC 
CAGGCCAAGA GGCAGGAGGA GGACTCGGAG AGCAGCAGCG AGGAGGAGTC GGACAGTGAC 
GGGGACGTGC CAGCTGCTGC ACCTCCAGCC CAGAAAGAGG GTAGCTCCAA AACTGCCACA 
AGCAAGACCC TGGCCCCAGC GTCCCTGGAG AAGAAAGCAG AGGAGTCCTC AACCAGCAGT 
GATGAGGACC TGCCATCCAG CCAGGTGATT AAAACCCCTC TGATTTTTGT CGACCCTAAT 
CGTAGTCCAG CTGGTCCAGC TGCTACCCCC GCCCAAGCGC AGGCTACAGG CACCCTGAGG 
AAGGCTCAGG CCTTGGAGAG CACAGCCAGG AGCTCCTCTG AGAGCGAGGA CGAGGACGTG 
ATCCCCGCTA CACAGTGCCC GACTCCTGCC ACTAGGACCA GCGTGGTGAC TGTGCCCACG 
GCACCCCCAA AGGCGGCCCT CAGAGCCAGT GTGGTGGGGG CTCCCAGCAG TGAGGACACA 
AGTCAGGGGT CTGAAGGCAA GAAGCAGGAG GCCTCAGCCA CTCAGGTGAC CAAGAGGAAC 
CTCCCGCTGA CACAGGCTGC CCTGAAGGTT CTTGCCCAGA AAGCCAGTGA GGCCCAATCT 
CCTGCTGCCA AGACCCCGTC TTCAAGTGGG ACTGACAGTG CTGTGGGAAC ACTCTCCACG 
GCAAGTCCCC AGAGCACCCC TACCCAGGTC AGAATGGCCA ACAAGCTCAG AAAGCCTGAG 
GTTCCTACAG CTCAGCAGGC CACAGCCACT CCCTCGGGAC ACCCCACAGC CAAGGCCCCT 
GCAACCTCAG ATGACAGCAA CAGCAGCTCT TCAGGGAGCG AGGACGATGC CAAGGGGTCC 
CAGGCGGCCC CGTCAGCCCA CAGGCCAGGC CCAGCCCCCT CCAGGAGGGA GACCTTGGTG 
GAAGAGACCA CAACAGAGTC CAGTGACGAC GAGGTGGTGG CACCTTCCCA GTCTCTCCTC 
TCAGGTTTTG TGACTCCTGG GCTGACTCCA GCTGGTTCCA AGACTTCAAA GGCCGCTCCC 
AAACCAGACG CCAGCCCCTC TGTTTCCTCT ACTCCGGCCA CCAGAGATGC CCCAGAGGGC 
AAGCAGGAAG TGGAGCCCCA ACAAGCAGCA GGCACCATGT CCCCTAAAAC AGGCAGAAAA 
GAGGCTGGTG CCACACCTCA GAAGCCCGGG AAGGGGCTCG GGAGTCCCCC GGCCTCCATG 
CTGGCGCTGC AGAGCAACAT CACCCAGCGC CTCCTGAGCG AGCCCTGGCC CCTGAGCGAG 
GCGCAGGTGC AGGCCTCGGT GGCGAAGGTG CTCACGGAGC TGCTGGAGCA GGAGAGGAAG 
AAGGCCATGG ACACCGCCAA GGAAGGCAGC CGGAAGGGCC GCCTGGGCCA TAAGCGGAAG 
CTGTCCGAAG ACCAGACAGC CCCCAAGGCC CCCAAGAACA AGAAAAAGAA GCAGCTGGCA 
GCTGCAGATG GTGGGGAGCA TGTCGTCTCC TCAGAAAAGG CCCCCAGGAC TGTCAAAGGG 
AAATCCAAGA AGGACAGAGC AAGTGGTGAC GTCAAGGAGA AGAAGGAGAA GGAGTCTCCC 
GGCTCTCAAG GGGCCAAGGA GAAGCCAGTA GGGGAGCTGG GGACTCCAAA GGGTGACGGG 
GGAGACCACG GCAACCTTAA GATGAAGAAA GAGAAGAAGA AATCTGACAA GAAAAAAAAA 
GACAAGGAGA AAAAGGAAAA GAAGAAGAAA GCAAAAAAGG CCTCAACCAA AGACCCTGGC 
TCACCATCAC AGAAGAAAAA GAAGAGAAAG AAGAAGACAG CAGAGCAAAC CGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_010807517.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010805819.1 Bos taurus Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant X4, mRNA.

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CloneID	OBa05568
Clone ID Related Accession (Same CDS sequence)	XM_002689293.5 , XM_010807517.2 , XM_591291.9 , XM_010798510.2
Accession Version	XM_010807517.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4377bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	treacle protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104039.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010807517.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAGG CCAGGAAGCG GCGAGAGCTC CTTCCCCTGA TCTACCAGCA TCTGCTGCAG 
GCGGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA AATTTTCCTG 
AGTCAGCCTG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACTTC GGAGATTGGC 
CGGAAACGGA AGGCAGAGGA AGATGCGGCC TTGCAGGCCA AGAGGAGCCG TGTGTCAGAC 
CCCATCAGCA GCTCAGAAAG CTCAGAAGAG GAGGAGGAAG AGGAGGAGGC AGAAGCCAAA 
ACCACCAAAC CCACCCCGAG ATTAGCAGCT ACCAACTCCT CAGTCACAGG GACAGTTTCG 
TCTTCAAATG TGAAAGAAAA AGCCAAGGCA AAGACCACGA AAGCCAGCAA GACGGTGAAC 
TCCACGACAC ACCCTGCCCC GGGAAAGGCA GTGGCCCACC TCCTCACTGG GAAGTCACCC 
CAGAAGACAT CGGGGCCCTC GGCAAATGCT GTCTTGGTGT CAGAAACCGA GGAGGAGGGC 
AGCGTGTCAG CCCTCAGAAC CGCCACCACG CCTGGAATGG CATCCGCCGA CCAGGCTGAC 
AGCTCCAGTG AGGATACCAC CAGCTCGAGT GATGAGACGG ACTTGGAGGC GAAAGCCGCA 
GTAAAACCAC TGCAGGTCAG AGCCTCAGCC ACTGCAGTCA AGGAGTCTCC GAGAAAAAGT 
GCAGCCCCGA CTCCAGGGAA GGCAGGGGTT GTGACACCCC AAGTCAGAGG CGGTGCTGTG 
ACCCCAGCCA GCAGGGCCAA GAAGCCAGAA GAGGTCTCAG AGAGCAGTGA AGAGTCCGAC 
GAGAGTGAGG AGGCCGCCCC TGCGGGGACA CCCAGTCAGG TGAAGGCCTT GGAGAAAACC 
GTCCCAATCA GAGTTGCTCC TAGTCCTGCC AAGGGGACCC CTGGGAAAGG GGCCACCCCA 
GCACCCCCTG GGAAGGCAGG GCCCTTAGGG AAGCCAGAGG AGGACTCGGA GAGCAGCAGC 
GAGGAGTCAG ACAGCGAGGA GGAGACACAA GCTGTCAAGA CCCCAGTGCA GGCAAAGCCC 
TCTGGGAAGA TCCCCCAGGT TAAAGCTGCC TCCACACCAA AAATCCAGGT GTTTTTCCCT 
AAGAAAGGGT CCCCTCCAAC ATCCCCTGGC AAGGCAGGGC CTGCAGCCAC CCAAGCCCAG 
CCCAGGAAGC AGGAGGAGGA GGAGAGCAGC AGCAGCAGCA GCAAGGAATC AGACAGCGAG 
GGCGAGGTGC CCACGGCCAT GCAGCAGACT ACAAGCCTGG CTCAGGCAAA GCCCTTGGAG 
AAAAACTCTC AGGTCAGAGC TGCCTCAGCC ACAGGTGCAG GACCCTCGGC AAAGGGCACG 
GTCCCAGCGC TCCCTCAGAA GGCAAGGTCT GTGGCCGCCC AGGATGGGAA GCAGGAGGAC 
TCAGAGAGCA GCAGTGAGGA GGAGTCAGAC AGCGAGGAGG AGATGCAGAA GGCAGGGACC 
TCAGCCCAGG CACAGTCCTC TGGGAAAGCC CTCCAGATCA GACCTGCCTC CGGTCCCACC 
AAGAGGCCCC CTCAGAAGGC CGGGCCTGCA ACCACCCAGG TCAAGGTTGA AAGGGCCGAG 
GAAGACTCGG AGAGCAGTGA GGAGGAATCA GACAGTGAGG ATGAAGCTCC TGTAGCCAAG 
ACTCCAGCCC AGGCAAAATC AGCTGTGAAA ACTTCTCAGA TCAAGGCCTC CCCAAGGAAG 
GGCACCCCCA TTACACCCTC ATCTGCCAGG GTCCCCCCAG TGCAAGTCGG CACACCAGCC 
CCCCGGACAG CAGCAGCAGT GAGTACGCCC ACCTGCGCGT CATCCCCAGC CATAGTCAGG 
CACACCCAGA AGCCAGGGGA GGACTCTTCA AGCAGCGAGG AGTCCGAGAG CGAGGAGGAG 
ACAGCTCCTG CTGCAGCAGG GGGACAGATG AAGTCTATAG GGAAGACCCT CCCAGTGAAA 
GCTGCCTCAC CAGCCACCAA GGGGCCCTCA GGAAAAGGGA CCACCCCAGG GCCCCCTGGG 
AAGGCAGGAC CTGCAGTGGC ACAGGTCAAG GCTGAGGTGC AGGAAGACTC AGAAAGCAGT 
GAGGAGTCGG ACAATGAGGA AGCGGCTGCA ACTCCCGCAC AGGTGAAGAC TTCAGTGAAA 
ACCCCTCAGA CCAAAGCCAA CTCTTCTGCC ACCAGAGTGG CTTCAGCCAA AGCAGCAGCA 
CCAGCTCCTG GAAAGGGGGG CCTTGCTGTT GCTCAAGCCA AAGTGGGGTC CCCAGCCAAG 
GTAAAACCAC CAGCGAGAAC CCCCCAGAGT AGCGCCGTCT CTGGGAGGGG CCAGGTGTCT 
GTGCCGGCTG TGGGGAAGGC AGCAGCTGCA GCAGCCCAGG CCCAGCCGGG GCCTGTCAAG 
GGCTCACAGG AGGACTCGGA GAGCAGTGAG GAGGAGTCGG ACAGTGAGGG AGAGGCACCT 
CCTCAGGGGA AGCCCTCAGG AAAGACCCCC CAGGTCAGAA CTGCCTCAGC CCCCACCAAG 
GCGTCCCCCA AGAAAGGGGC TGCCCCAGTA CCCCCTGGGA AAGCAGGACC CCCAGCCACC 
CAGGCCAAGA GGCAGGAGGA GGACTCGGAG AGCAGCAGCG AGGAGGAGTC GGACAGTGAC 
GGGGACGTGC CAGCTGCTGC ACCTCCAGCC CAGAAAGAGG GTAGCTCCAA AACTGCCACA 
AGCAAGACCC TGGCCCCAGC GTCCCTGGAG AAGAAAGCAG AGGAGTCCTC AACCAGCAGT 
GATGAGGACC TGCCATCCAG CCAGGTGATT AAAACCCCTC TGATTTTTGT CGACCCTAAT 
CGTAGTCCAG CTGGTCCAGC TGCTACCCCC GCCCAAGCGC AGGCTACAGG CACCCTGAGG 
AAGGCTCAGG CCTTGGAGAG CACAGCCAGG AGCTCCTCTG AGAGCGAGGA CGAGGACGTG 
ATCCCCGCTA CACAGTGCCC GACTCCTGCC ACTAGGACCA GCGTGGTGAC TGTGCCCACG 
GCACCCCCAA AGGCGGCCCT CAGAGCCAGT GTGGTGGGGG CTCCCAGCAG TGAGGACACA 
AGTCAGGGGT CTGAAGGCAA GAAGCAGGAG GCCTCAGCCA CTCAGGTGAC CAAGAGGAAC 
CTCCCGCTGA CACAGGCTGC CCTGAAGGTT CTTGCCCAGA AAGCCAGTGA GGCCCAATCT 
CCTGCTGCCA AGACCCCGTC TTCAAGTGGG ACTGACAGTG CTGTGGGAAC ACTCTCCACG 
GCAAGTCCCC AGAGCACCCC TACCCAGGTC AGAATGGCCA ACAAGCTCAG AAAGCCTGAG 
GTTCCTACAG CTCAGCAGGC CACAGCCACT CCCTCGGGAC ACCCCACAGC CAAGGCCCCT 
GCAACCTCAG ATGACAGCAA CAGCAGCTCT TCAGGGAGCG AGGACGATGC CAAGGGGTCC 
CAGGCGGCCC CGTCAGCCCA CAGGCCAGGC CCAGCCCCCT CCAGGAGGGA GACCTTGGTG 
GAAGAGACCA CAACAGAGTC CAGTGACGAC GAGGTGGTGG CACCTTCCCA GTCTCTCCTC 
TCAGGTTTTG TGACTCCTGG GCTGACTCCA GCTGGTTCCA AGACTTCAAA GGCCGCTCCC 
AAACCAGACG CCAGCCCCTC TGTTTCCTCT ACTCCGGCCA CCAGAGATGC CCCAGAGGGC 
AAGCAGGAAG TGGAGCCCCA ACAAGCAGCA GGCACCATGT CCCCTAAAAC AGGCAGAAAA 
GAGGCTGGTG CCACACCTCA GAAGCCCGGG AAGGGGCTCG GGAGTCCCCC GGCCTCCATG 
CTGGCGCTGC AGAGCAACAT CACCCAGCGC CTCCTGAGCG AGCCCTGGCC CCTGAGCGAG 
GCGCAGGTGC AGGCCTCGGT GGCGAAGGTG CTCACGGAGC TGCTGGAGCA GGAGAGGAAG 
AAGGCCATGG ACACCGCCAA GGAAGGCAGC CGGAAGGGCC GCCTGGGCCA TAAGCGGAAG 
CTGTCCGAAG ACCAGACAGC CCCCAAGGCC CCCAAGAACA AGAAAAAGAA GCAGCTGGCA 
GCTGCAGATG GTGGGGAGCA TGTCGTCTCC TCAGAAAAGG CCCCCAGGAC TGTCAAAGGG 
AAATCCAAGA AGGACAGAGC AAGTGGTGAC GTCAAGGAGA AGAAGGAGAA GGAGTCTCCC 
GGCTCTCAAG GGGCCAAGGA GAAGCCAGTA GGGGAGCTGG GGACTCCAAA GGGTGACGGG 
GGAGACCACG GCAACCTTAA GATGAAGAAA GAGAAGAAGA AATCTGACAA GAAAAAAAAA 
GACAAGGAGA AAAAGGAAAA GAAGAAGAAA GCAAAAAAGG CCTCAACCAA AGACCCTGGC 
TCACCATCAC AGAAGAAAAA GAAGAGAAAG AAGAAGACAG CAGAGCAAAC CGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010805819.1
CDS	59..4435
Translation	MAEARKRRELLPLIYQHLLQAGYVRAAREVKEQSGQKIFLSQPVTLLDIYTHWQQTSEIGRKRKAEEDAALQAKRSRVSDPISSSESSEEEEEEEEAEAKTTKPTPRLAATNSSVTGTVSSSNVKEKAKAKTTKASKTVNSTTHPAPGKAVAHLLTGKSPQKTSGPSANAVLVSETEEEGSVSALRTATTPGMASADQADSSSEDTTSSSDETDLEAKAAVKPLQVRASATAVKESPRKSAAPTPGKAGVVTPQVRGGAVTPASRAKKPEEVSESSEESDESEEAAPAGTPSQVKALEKTVPIRVAPSPAKGTPGKGATPAPPGKAGPLGKPEEDSESSSEESDSEEETQAVKTPVQAKPSGKIPQVKAASTPKIQVFFPKKGSPPTSPGKAGPAATQAQPRKQEEEESSSSSSKESDSEGEVPTAMQQTTSLAQAKPLEKNSQVRAASATGAGPSAKGTVPALPQKARSVAAQDGKQEDSESSSEEESDSEEEMQKAGTSAQAQSSGKALQIRPASGPTKRPPQKAGPATTQVKVERAEEDSESSEEESDSEDEAPVAKTPAQAKSAVKTSQIKASPRKGTPITPSSARVPPVQVGTPAPRTAAAVSTPTCASSPAIVRHTQKPGEDSSSSEESESEEETAPAAAGGQMKSIGKTLPVKAASPATKGPSGKGTTPGPPGKAGPAVAQVKAEVQEDSESSEESDNEEAAATPAQVKTSVKTPQTKANSSATRVASAKAAAPAPGKGGLAVAQAKVGSPAKVKPPARTPQSSAVSGRGQVSVPAVGKAAAAAAQAQPGPVKGSQEDSESSEEESDSEGEAPPQGKPSGKTPQVRTASAPTKASPKKGAAPVPPGKAGPPATQAKRQEEDSESSSEEESDSDGDVPAAAPPAQKEGSSKTATSKTLAPASLEKKAEESSTSSDEDLPSSQVIKTPLIFVDPNRSPAGPAATPAQAQATGTLRKAQALESTARSSSESEDEDVIPATQCPTPATRTSVVTVPTAPPKAALRASVVGAPSSEDTSQGSEGKKQEASATQVTKRNLPLTQAALKVLAQKASEAQSPAAKTPSSSGTDSAVGTLSTASPQSTPTQVRMANKLRKPEVPTAQQATATPSGHPTAKAPATSDDSNSSSSGSEDDAKGSQAAPSAHRPGPAPSRRETLVEETTTESSDDEVVAPSQSLLSGFVTPGLTPAGSKTSKAAPKPDASPSVSSTPATRDAPEGKQEVEPQQAAGTMSPKTGRKEAGATPQKPGKGLGSPPASMLALQSNITQRLLSEPWPLSEAQVQASVAKVLTELLEQERKKAMDTAKEGSRKGRLGHKRKLSEDQTAPKAPKNKKKKQLAAADGGEHVVSSEKAPRTVKGKSKKDRASGDVKEKKEKESPGSQGAKEKPVGELGTPKGDGGDHGNLKMKKEKKKSDKKKKDKEKKEKKKKAKKASTKDPGSPSQKKKKRKKKTAEQTV

Target ORF information:

RefSeq Version	XM_010807517.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010807517.2

ORF Insert Sequence:

ATGGCTGAGG CCAGGAAGCG GCGAGAGCTC CTTCCCCTGA TCTACCAGCA TCTGCTGCAG 
GCGGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA AATTTTCCTG 
AGTCAGCCTG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACTTC GGAGATTGGC 
CGGAAACGGA AGGCAGAGGA AGATGCGGCC TTGCAGGCCA AGAGGAGCCG TGTGTCAGAC 
CCCATCAGCA GCTCAGAAAG CTCAGAAGAG GAGGAGGAAG AGGAGGAGGC AGAAGCCAAA 
ACCACCAAAC CCACCCCGAG ATTAGCAGCT ACCAACTCCT CAGTCACAGG GACAGTTTCG 
TCTTCAAATG TGAAAGAAAA AGCCAAGGCA AAGACCACGA AAGCCAGCAA GACGGTGAAC 
TCCACGACAC ACCCTGCCCC GGGAAAGGCA GTGGCCCACC TCCTCACTGG GAAGTCACCC 
CAGAAGACAT CGGGGCCCTC GGCAAATGCT GTCTTGGTGT CAGAAACCGA GGAGGAGGGC 
AGCGTGTCAG CCCTCAGAAC CGCCACCACG CCTGGAATGG CATCCGCCGA CCAGGCTGAC 
AGCTCCAGTG AGGATACCAC CAGCTCGAGT GATGAGACGG ACTTGGAGGC GAAAGCCGCA 
GTAAAACCAC TGCAGGTCAG AGCCTCAGCC ACTGCAGTCA AGGAGTCTCC GAGAAAAAGT 
GCAGCCCCGA CTCCAGGGAA GGCAGGGGTT GTGACACCCC AAGTCAGAGG CGGTGCTGTG 
ACCCCAGCCA GCAGGGCCAA GAAGCCAGAA GAGGTCTCAG AGAGCAGTGA AGAGTCCGAC 
GAGAGTGAGG AGGCCGCCCC TGCGGGGACA CCCAGTCAGG TGAAGGCCTT GGAGAAAACC 
GTCCCAATCA GAGTTGCTCC TAGTCCTGCC AAGGGGACCC CTGGGAAAGG GGCCACCCCA 
GCACCCCCTG GGAAGGCAGG GCCCTTAGGG AAGCCAGAGG AGGACTCGGA GAGCAGCAGC 
GAGGAGTCAG ACAGCGAGGA GGAGACACAA GCTGTCAAGA CCCCAGTGCA GGCAAAGCCC 
TCTGGGAAGA TCCCCCAGGT TAAAGCTGCC TCCACACCAA AAATCCAGGT GTTTTTCCCT 
AAGAAAGGGT CCCCTCCAAC ATCCCCTGGC AAGGCAGGGC CTGCAGCCAC CCAAGCCCAG 
CCCAGGAAGC AGGAGGAGGA GGAGAGCAGC AGCAGCAGCA GCAAGGAATC AGACAGCGAG 
GGCGAGGTGC CCACGGCCAT GCAGCAGACT ACAAGCCTGG CTCAGGCAAA GCCCTTGGAG 
AAAAACTCTC AGGTCAGAGC TGCCTCAGCC ACAGGTGCAG GACCCTCGGC AAAGGGCACG 
GTCCCAGCGC TCCCTCAGAA GGCAAGGTCT GTGGCCGCCC AGGATGGGAA GCAGGAGGAC 
TCAGAGAGCA GCAGTGAGGA GGAGTCAGAC AGCGAGGAGG AGATGCAGAA GGCAGGGACC 
TCAGCCCAGG CACAGTCCTC TGGGAAAGCC CTCCAGATCA GACCTGCCTC CGGTCCCACC 
AAGAGGCCCC CTCAGAAGGC CGGGCCTGCA ACCACCCAGG TCAAGGTTGA AAGGGCCGAG 
GAAGACTCGG AGAGCAGTGA GGAGGAATCA GACAGTGAGG ATGAAGCTCC TGTAGCCAAG 
ACTCCAGCCC AGGCAAAATC AGCTGTGAAA ACTTCTCAGA TCAAGGCCTC CCCAAGGAAG 
GGCACCCCCA TTACACCCTC ATCTGCCAGG GTCCCCCCAG TGCAAGTCGG CACACCAGCC 
CCCCGGACAG CAGCAGCAGT GAGTACGCCC ACCTGCGCGT CATCCCCAGC CATAGTCAGG 
CACACCCAGA AGCCAGGGGA GGACTCTTCA AGCAGCGAGG AGTCCGAGAG CGAGGAGGAG 
ACAGCTCCTG CTGCAGCAGG GGGACAGATG AAGTCTATAG GGAAGACCCT CCCAGTGAAA 
GCTGCCTCAC CAGCCACCAA GGGGCCCTCA GGAAAAGGGA CCACCCCAGG GCCCCCTGGG 
AAGGCAGGAC CTGCAGTGGC ACAGGTCAAG GCTGAGGTGC AGGAAGACTC AGAAAGCAGT 
GAGGAGTCGG ACAATGAGGA AGCGGCTGCA ACTCCCGCAC AGGTGAAGAC TTCAGTGAAA 
ACCCCTCAGA CCAAAGCCAA CTCTTCTGCC ACCAGAGTGG CTTCAGCCAA AGCAGCAGCA 
CCAGCTCCTG GAAAGGGGGG CCTTGCTGTT GCTCAAGCCA AAGTGGGGTC CCCAGCCAAG 
GTAAAACCAC CAGCGAGAAC CCCCCAGAGT AGCGCCGTCT CTGGGAGGGG CCAGGTGTCT 
GTGCCGGCTG TGGGGAAGGC AGCAGCTGCA GCAGCCCAGG CCCAGCCGGG GCCTGTCAAG 
GGCTCACAGG AGGACTCGGA GAGCAGTGAG GAGGAGTCGG ACAGTGAGGG AGAGGCACCT 
CCTCAGGGGA AGCCCTCAGG AAAGACCCCC CAGGTCAGAA CTGCCTCAGC CCCCACCAAG 
GCGTCCCCCA AGAAAGGGGC TGCCCCAGTA CCCCCTGGGA AAGCAGGACC CCCAGCCACC 
CAGGCCAAGA GGCAGGAGGA GGACTCGGAG AGCAGCAGCG AGGAGGAGTC GGACAGTGAC 
GGGGACGTGC CAGCTGCTGC ACCTCCAGCC CAGAAAGAGG GTAGCTCCAA AACTGCCACA 
AGCAAGACCC TGGCCCCAGC GTCCCTGGAG AAGAAAGCAG AGGAGTCCTC AACCAGCAGT 
GATGAGGACC TGCCATCCAG CCAGGTGATT AAAACCCCTC TGATTTTTGT CGACCCTAAT 
CGTAGTCCAG CTGGTCCAGC TGCTACCCCC GCCCAAGCGC AGGCTACAGG CACCCTGAGG 
AAGGCTCAGG CCTTGGAGAG CACAGCCAGG AGCTCCTCTG AGAGCGAGGA CGAGGACGTG 
ATCCCCGCTA CACAGTGCCC GACTCCTGCC ACTAGGACCA GCGTGGTGAC TGTGCCCACG 
GCACCCCCAA AGGCGGCCCT CAGAGCCAGT GTGGTGGGGG CTCCCAGCAG TGAGGACACA 
AGTCAGGGGT CTGAAGGCAA GAAGCAGGAG GCCTCAGCCA CTCAGGTGAC CAAGAGGAAC 
CTCCCGCTGA CACAGGCTGC CCTGAAGGTT CTTGCCCAGA AAGCCAGTGA GGCCCAATCT 
CCTGCTGCCA AGACCCCGTC TTCAAGTGGG ACTGACAGTG CTGTGGGAAC ACTCTCCACG 
GCAAGTCCCC AGAGCACCCC TACCCAGGTC AGAATGGCCA ACAAGCTCAG AAAGCCTGAG 
GTTCCTACAG CTCAGCAGGC CACAGCCACT CCCTCGGGAC ACCCCACAGC CAAGGCCCCT 
GCAACCTCAG ATGACAGCAA CAGCAGCTCT TCAGGGAGCG AGGACGATGC CAAGGGGTCC 
CAGGCGGCCC CGTCAGCCCA CAGGCCAGGC CCAGCCCCCT CCAGGAGGGA GACCTTGGTG 
GAAGAGACCA CAACAGAGTC CAGTGACGAC GAGGTGGTGG CACCTTCCCA GTCTCTCCTC 
TCAGGTTTTG TGACTCCTGG GCTGACTCCA GCTGGTTCCA AGACTTCAAA GGCCGCTCCC 
AAACCAGACG CCAGCCCCTC TGTTTCCTCT ACTCCGGCCA CCAGAGATGC CCCAGAGGGC 
AAGCAGGAAG TGGAGCCCCA ACAAGCAGCA GGCACCATGT CCCCTAAAAC AGGCAGAAAA 
GAGGCTGGTG CCACACCTCA GAAGCCCGGG AAGGGGCTCG GGAGTCCCCC GGCCTCCATG 
CTGGCGCTGC AGAGCAACAT CACCCAGCGC CTCCTGAGCG AGCCCTGGCC CCTGAGCGAG 
GCGCAGGTGC AGGCCTCGGT GGCGAAGGTG CTCACGGAGC TGCTGGAGCA GGAGAGGAAG 
AAGGCCATGG ACACCGCCAA GGAAGGCAGC CGGAAGGGCC GCCTGGGCCA TAAGCGGAAG 
CTGTCCGAAG ACCAGACAGC CCCCAAGGCC CCCAAGAACA AGAAAAAGAA GCAGCTGGCA 
GCTGCAGATG GTGGGGAGCA TGTCGTCTCC TCAGAAAAGG CCCCCAGGAC TGTCAAAGGG 
AAATCCAAGA AGGACAGAGC AAGTGGTGAC GTCAAGGAGA AGAAGGAGAA GGAGTCTCCC 
GGCTCTCAAG GGGCCAAGGA GAAGCCAGTA GGGGAGCTGG GGACTCCAAA GGGTGACGGG 
GGAGACCACG GCAACCTTAA GATGAAGAAA GAGAAGAAGA AATCTGACAA GAAAAAAAAA 
GACAAGGAGA AAAAGGAAAA GAAGAAGAAA GCAAAAAAGG CCTCAACCAA AGACCCTGGC 
TCACCATCAC AGAAGAAAAA GAAGAGAAAG AAGAAGACAG CAGAGCAAAC CGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_591291.9 ) cDNA ORF clone, Bos taurus(cattle) -> XP_591291.5 Bos taurus Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa05568
Clone ID Related Accession (Same CDS sequence)	XM_002689293.5 , XM_010807517.2 , XM_591291.9 , XM_010798510.2
Accession Version	XM_591291.9 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4377bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	treacle protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645112.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_591291.8. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAGG CCAGGAAGCG GCGAGAGCTC CTTCCCCTGA TCTACCAGCA TCTGCTGCAG 
GCGGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA AATTTTCCTG 
AGTCAGCCTG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACTTC GGAGATTGGC 
CGGAAACGGA AGGCAGAGGA AGATGCGGCC TTGCAGGCCA AGAGGAGCCG TGTGTCAGAC 
CCCATCAGCA GCTCAGAAAG CTCAGAAGAG GAGGAGGAAG AGGAGGAGGC AGAAGCCAAA 
ACCACCAAAC CCACCCCGAG ATTAGCAGCT ACCAACTCCT CAGTCACAGG GACAGTTTCG 
TCTTCAAATG TGAAAGAAAA AGCCAAGGCA AAGACCACGA AAGCCAGCAA GACGGTGAAC 
TCCACGACAC ACCCTGCCCC GGGAAAGGCA GTGGCCCACC TCCTCACTGG GAAGTCACCC 
CAGAAGACAT CGGGGCCCTC GGCAAATGCT GTCTTGGTGT CAGAAACCGA GGAGGAGGGC 
AGCGTGTCAG CCCTCAGAAC CGCCACCACG CCTGGAATGG CATCCGCCGA CCAGGCTGAC 
AGCTCCAGTG AGGATACCAC CAGCTCGAGT GATGAGACGG ACTTGGAGGC GAAAGCCGCA 
GTAAAACCAC TGCAGGTCAG AGCCTCAGCC ACTGCAGTCA AGGAGTCTCC GAGAAAAAGT 
GCAGCCCCGA CTCCAGGGAA GGCAGGGGTT GTGACACCCC AAGTCAGAGG CGGTGCTGTG 
ACCCCAGCCA GCAGGGCCAA GAAGCCAGAA GAGGTCTCAG AGAGCAGTGA AGAGTCCGAC 
GAGAGTGAGG AGGCCGCCCC TGCGGGGACA CCCAGTCAGG TGAAGGCCTT GGAGAAAACC 
GTCCCAATCA GAGTTGCTCC TAGTCCTGCC AAGGGGACCC CTGGGAAAGG GGCCACCCCA 
GCACCCCCTG GGAAGGCAGG GCCCTTAGGG AAGCCAGAGG AGGACTCGGA GAGCAGCAGC 
GAGGAGTCAG ACAGCGAGGA GGAGACACAA GCTGTCAAGA CCCCAGTGCA GGCAAAGCCC 
TCTGGGAAGA TCCCCCAGGT TAAAGCTGCC TCCACACCAA AAATCCAGGT GTTTTTCCCT 
AAGAAAGGGT CCCCTCCAAC ATCCCCTGGC AAGGCAGGGC CTGCAGCCAC CCAAGCCCAG 
CCCAGGAAGC AGGAGGAGGA GGAGAGCAGC AGCAGCAGCA GCAAGGAATC AGACAGCGAG 
GGCGAGGTGC CCACGGCCAT GCAGCAGACT ACAAGCCTGG CTCAGGCAAA GCCCTTGGAG 
AAAAACTCTC AGGTCAGAGC TGCCTCAGCC ACAGGTGCAG GACCCTCGGC AAAGGGCACG 
GTCCCAGCGC TCCCTCAGAA GGCAAGGTCT GTGGCCGCCC AGGATGGGAA GCAGGAGGAC 
TCAGAGAGCA GCAGTGAGGA GGAGTCAGAC AGCGAGGAGG AGATGCAGAA GGCAGGGACC 
TCAGCCCAGG CACAGTCCTC TGGGAAAGCC CTCCAGATCA GACCTGCCTC CGGTCCCACC 
AAGAGGCCCC CTCAGAAGGC CGGGCCTGCA ACCACCCAGG TCAAGGTTGA AAGGGCCGAG 
GAAGACTCGG AGAGCAGTGA GGAGGAATCA GACAGTGAGG ATGAAGCTCC TGTAGCCAAG 
ACTCCAGCCC AGGCAAAATC AGCTGTGAAA ACTTCTCAGA TCAAGGCCTC CCCAAGGAAG 
GGCACCCCCA TTACACCCTC ATCTGCCAGG GTCCCCCCAG TGCAAGTCGG CACACCAGCC 
CCCCGGACAG CAGCAGCAGT GAGTACGCCC ACCTGCGCGT CATCCCCAGC CATAGTCAGG 
CACACCCAGA AGCCAGGGGA GGACTCTTCA AGCAGCGAGG AGTCCGAGAG CGAGGAGGAG 
ACAGCTCCTG CTGCAGCAGG GGGACAGATG AAGTCTATAG GGAAGACCCT CCCAGTGAAA 
GCTGCCTCAC CAGCCACCAA GGGGCCCTCA GGAAAAGGGA CCACCCCAGG GCCCCCTGGG 
AAGGCAGGAC CTGCAGTGGC ACAGGTCAAG GCTGAGGTGC AGGAAGACTC AGAAAGCAGT 
GAGGAGTCGG ACAATGAGGA AGCGGCTGCA ACTCCCGCAC AGGTGAAGAC TTCAGTGAAA 
ACCCCTCAGA CCAAAGCCAA CTCTTCTGCC ACCAGAGTGG CTTCAGCCAA AGCAGCAGCA 
CCAGCTCCTG GAAAGGGGGG CCTTGCTGTT GCTCAAGCCA AAGTGGGGTC CCCAGCCAAG 
GTAAAACCAC CAGCGAGAAC CCCCCAGAGT AGCGCCGTCT CTGGGAGGGG CCAGGTGTCT 
GTGCCGGCTG TGGGGAAGGC AGCAGCTGCA GCAGCCCAGG CCCAGCCGGG GCCTGTCAAG 
GGCTCACAGG AGGACTCGGA GAGCAGTGAG GAGGAGTCGG ACAGTGAGGG AGAGGCACCT 
CCTCAGGGGA AGCCCTCAGG AAAGACCCCC CAGGTCAGAA CTGCCTCAGC CCCCACCAAG 
GCGTCCCCCA AGAAAGGGGC TGCCCCAGTA CCCCCTGGGA AAGCAGGACC CCCAGCCACC 
CAGGCCAAGA GGCAGGAGGA GGACTCGGAG AGCAGCAGCG AGGAGGAGTC GGACAGTGAC 
GGGGACGTGC CAGCTGCTGC ACCTCCAGCC CAGAAAGAGG GTAGCTCCAA AACTGCCACA 
AGCAAGACCC TGGCCCCAGC GTCCCTGGAG AAGAAAGCAG AGGAGTCCTC AACCAGCAGT 
GATGAGGACC TGCCATCCAG CCAGGTGATT AAAACCCCTC TGATTTTTGT CGACCCTAAT 
CGTAGTCCAG CTGGTCCAGC TGCTACCCCC GCCCAAGCGC AGGCTACAGG CACCCTGAGG 
AAGGCTCAGG CCTTGGAGAG CACAGCCAGG AGCTCCTCTG AGAGCGAGGA CGAGGACGTG 
ATCCCCGCTA CACAGTGCCC GACTCCTGCC ACTAGGACCA GCGTGGTGAC TGTGCCCACG 
GCACCCCCAA AGGCGGCCCT CAGAGCCAGT GTGGTGGGGG CTCCCAGCAG TGAGGACACA 
AGTCAGGGGT CTGAAGGCAA GAAGCAGGAG GCCTCAGCCA CTCAGGTGAC CAAGAGGAAC 
CTCCCGCTGA CACAGGCTGC CCTGAAGGTT CTTGCCCAGA AAGCCAGTGA GGCCCAATCT 
CCTGCTGCCA AGACCCCGTC TTCAAGTGGG ACTGACAGTG CTGTGGGAAC ACTCTCCACG 
GCAAGTCCCC AGAGCACCCC TACCCAGGTC AGAATGGCCA ACAAGCTCAG AAAGCCTGAG 
GTTCCTACAG CTCAGCAGGC CACAGCCACT CCCTCGGGAC ACCCCACAGC CAAGGCCCCT 
GCAACCTCAG ATGACAGCAA CAGCAGCTCT TCAGGGAGCG AGGACGATGC CAAGGGGTCC 
CAGGCGGCCC CGTCAGCCCA CAGGCCAGGC CCAGCCCCCT CCAGGAGGGA GACCTTGGTG 
GAAGAGACCA CAACAGAGTC CAGTGACGAC GAGGTGGTGG CACCTTCCCA GTCTCTCCTC 
TCAGGTTTTG TGACTCCTGG GCTGACTCCA GCTGGTTCCA AGACTTCAAA GGCCGCTCCC 
AAACCAGACG CCAGCCCCTC TGTTTCCTCT ACTCCGGCCA CCAGAGATGC CCCAGAGGGC 
AAGCAGGAAG TGGAGCCCCA ACAAGCAGCA GGCACCATGT CCCCTAAAAC AGGCAGAAAA 
GAGGCTGGTG CCACACCTCA GAAGCCCGGG AAGGGGCTCG GGAGTCCCCC GGCCTCCATG 
CTGGCGCTGC AGAGCAACAT CACCCAGCGC CTCCTGAGCG AGCCCTGGCC CCTGAGCGAG 
GCGCAGGTGC AGGCCTCGGT GGCGAAGGTG CTCACGGAGC TGCTGGAGCA GGAGAGGAAG 
AAGGCCATGG ACACCGCCAA GGAAGGCAGC CGGAAGGGCC GCCTGGGCCA TAAGCGGAAG 
CTGTCCGAAG ACCAGACAGC CCCCAAGGCC CCCAAGAACA AGAAAAAGAA GCAGCTGGCA 
GCTGCAGATG GTGGGGAGCA TGTCGTCTCC TCAGAAAAGG CCCCCAGGAC TGTCAAAGGG 
AAATCCAAGA AGGACAGAGC AAGTGGTGAC GTCAAGGAGA AGAAGGAGAA GGAGTCTCCC 
GGCTCTCAAG GGGCCAAGGA GAAGCCAGTA GGGGAGCTGG GGACTCCAAA GGGTGACGGG 
GGAGACCACG GCAACCTTAA GATGAAGAAA GAGAAGAAGA AATCTGACAA GAAAAAAAAA 
GACAAGGAGA AAAAGGAAAA GAAGAAGAAA GCAAAAAAGG CCTCAACCAA AGACCCTGGC 
TCACCATCAC AGAAGAAAAA GAAGAGAAAG AAGAAGACAG CAGAGCAAAC CGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_591291.5
CDS	59..4435
Translation	MAEARKRRELLPLIYQHLLQAGYVRAAREVKEQSGQKIFLSQPVTLLDIYTHWQQTSEIGRKRKAEEDAALQAKRSRVSDPISSSESSEEEEEEEEAEAKTTKPTPRLAATNSSVTGTVSSSNVKEKAKAKTTKASKTVNSTTHPAPGKAVAHLLTGKSPQKTSGPSANAVLVSETEEEGSVSALRTATTPGMASADQADSSSEDTTSSSDETDLEAKAAVKPLQVRASATAVKESPRKSAAPTPGKAGVVTPQVRGGAVTPASRAKKPEEVSESSEESDESEEAAPAGTPSQVKALEKTVPIRVAPSPAKGTPGKGATPAPPGKAGPLGKPEEDSESSSEESDSEEETQAVKTPVQAKPSGKIPQVKAASTPKIQVFFPKKGSPPTSPGKAGPAATQAQPRKQEEEESSSSSSKESDSEGEVPTAMQQTTSLAQAKPLEKNSQVRAASATGAGPSAKGTVPALPQKARSVAAQDGKQEDSESSSEEESDSEEEMQKAGTSAQAQSSGKALQIRPASGPTKRPPQKAGPATTQVKVERAEEDSESSEEESDSEDEAPVAKTPAQAKSAVKTSQIKASPRKGTPITPSSARVPPVQVGTPAPRTAAAVSTPTCASSPAIVRHTQKPGEDSSSSEESESEEETAPAAAGGQMKSIGKTLPVKAASPATKGPSGKGTTPGPPGKAGPAVAQVKAEVQEDSESSEESDNEEAAATPAQVKTSVKTPQTKANSSATRVASAKAAAPAPGKGGLAVAQAKVGSPAKVKPPARTPQSSAVSGRGQVSVPAVGKAAAAAAQAQPGPVKGSQEDSESSEEESDSEGEAPPQGKPSGKTPQVRTASAPTKASPKKGAAPVPPGKAGPPATQAKRQEEDSESSSEEESDSDGDVPAAAPPAQKEGSSKTATSKTLAPASLEKKAEESSTSSDEDLPSSQVIKTPLIFVDPNRSPAGPAATPAQAQATGTLRKAQALESTARSSSESEDEDVIPATQCPTPATRTSVVTVPTAPPKAALRASVVGAPSSEDTSQGSEGKKQEASATQVTKRNLPLTQAALKVLAQKASEAQSPAAKTPSSSGTDSAVGTLSTASPQSTPTQVRMANKLRKPEVPTAQQATATPSGHPTAKAPATSDDSNSSSSGSEDDAKGSQAAPSAHRPGPAPSRRETLVEETTTESSDDEVVAPSQSLLSGFVTPGLTPAGSKTSKAAPKPDASPSVSSTPATRDAPEGKQEVEPQQAAGTMSPKTGRKEAGATPQKPGKGLGSPPASMLALQSNITQRLLSEPWPLSEAQVQASVAKVLTELLEQERKKAMDTAKEGSRKGRLGHKRKLSEDQTAPKAPKNKKKKQLAAADGGEHVVSSEKAPRTVKGKSKKDRASGDVKEKKEKESPGSQGAKEKPVGELGTPKGDGGDHGNLKMKKEKKKSDKKKKDKEKKEKKKKAKKASTKDPGSPSQKKKKRKKKTAEQTV

Target ORF information:

RefSeq Version	XM_591291.9
Organism	Bos taurus(cattle)
Definition	Bos taurus Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_591291.9

ORF Insert Sequence:

ATGGCTGAGG CCAGGAAGCG GCGAGAGCTC CTTCCCCTGA TCTACCAGCA TCTGCTGCAG 
GCGGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA AATTTTCCTG 
AGTCAGCCTG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACTTC GGAGATTGGC 
CGGAAACGGA AGGCAGAGGA AGATGCGGCC TTGCAGGCCA AGAGGAGCCG TGTGTCAGAC 
CCCATCAGCA GCTCAGAAAG CTCAGAAGAG GAGGAGGAAG AGGAGGAGGC AGAAGCCAAA 
ACCACCAAAC CCACCCCGAG ATTAGCAGCT ACCAACTCCT CAGTCACAGG GACAGTTTCG 
TCTTCAAATG TGAAAGAAAA AGCCAAGGCA AAGACCACGA AAGCCAGCAA GACGGTGAAC 
TCCACGACAC ACCCTGCCCC GGGAAAGGCA GTGGCCCACC TCCTCACTGG GAAGTCACCC 
CAGAAGACAT CGGGGCCCTC GGCAAATGCT GTCTTGGTGT CAGAAACCGA GGAGGAGGGC 
AGCGTGTCAG CCCTCAGAAC CGCCACCACG CCTGGAATGG CATCCGCCGA CCAGGCTGAC 
AGCTCCAGTG AGGATACCAC CAGCTCGAGT GATGAGACGG ACTTGGAGGC GAAAGCCGCA 
GTAAAACCAC TGCAGGTCAG AGCCTCAGCC ACTGCAGTCA AGGAGTCTCC GAGAAAAAGT 
GCAGCCCCGA CTCCAGGGAA GGCAGGGGTT GTGACACCCC AAGTCAGAGG CGGTGCTGTG 
ACCCCAGCCA GCAGGGCCAA GAAGCCAGAA GAGGTCTCAG AGAGCAGTGA AGAGTCCGAC 
GAGAGTGAGG AGGCCGCCCC TGCGGGGACA CCCAGTCAGG TGAAGGCCTT GGAGAAAACC 
GTCCCAATCA GAGTTGCTCC TAGTCCTGCC AAGGGGACCC CTGGGAAAGG GGCCACCCCA 
GCACCCCCTG GGAAGGCAGG GCCCTTAGGG AAGCCAGAGG AGGACTCGGA GAGCAGCAGC 
GAGGAGTCAG ACAGCGAGGA GGAGACACAA GCTGTCAAGA CCCCAGTGCA GGCAAAGCCC 
TCTGGGAAGA TCCCCCAGGT TAAAGCTGCC TCCACACCAA AAATCCAGGT GTTTTTCCCT 
AAGAAAGGGT CCCCTCCAAC ATCCCCTGGC AAGGCAGGGC CTGCAGCCAC CCAAGCCCAG 
CCCAGGAAGC AGGAGGAGGA GGAGAGCAGC AGCAGCAGCA GCAAGGAATC AGACAGCGAG 
GGCGAGGTGC CCACGGCCAT GCAGCAGACT ACAAGCCTGG CTCAGGCAAA GCCCTTGGAG 
AAAAACTCTC AGGTCAGAGC TGCCTCAGCC ACAGGTGCAG GACCCTCGGC AAAGGGCACG 
GTCCCAGCGC TCCCTCAGAA GGCAAGGTCT GTGGCCGCCC AGGATGGGAA GCAGGAGGAC 
TCAGAGAGCA GCAGTGAGGA GGAGTCAGAC AGCGAGGAGG AGATGCAGAA GGCAGGGACC 
TCAGCCCAGG CACAGTCCTC TGGGAAAGCC CTCCAGATCA GACCTGCCTC CGGTCCCACC 
AAGAGGCCCC CTCAGAAGGC CGGGCCTGCA ACCACCCAGG TCAAGGTTGA AAGGGCCGAG 
GAAGACTCGG AGAGCAGTGA GGAGGAATCA GACAGTGAGG ATGAAGCTCC TGTAGCCAAG 
ACTCCAGCCC AGGCAAAATC AGCTGTGAAA ACTTCTCAGA TCAAGGCCTC CCCAAGGAAG 
GGCACCCCCA TTACACCCTC ATCTGCCAGG GTCCCCCCAG TGCAAGTCGG CACACCAGCC 
CCCCGGACAG CAGCAGCAGT GAGTACGCCC ACCTGCGCGT CATCCCCAGC CATAGTCAGG 
CACACCCAGA AGCCAGGGGA GGACTCTTCA AGCAGCGAGG AGTCCGAGAG CGAGGAGGAG 
ACAGCTCCTG CTGCAGCAGG GGGACAGATG AAGTCTATAG GGAAGACCCT CCCAGTGAAA 
GCTGCCTCAC CAGCCACCAA GGGGCCCTCA GGAAAAGGGA CCACCCCAGG GCCCCCTGGG 
AAGGCAGGAC CTGCAGTGGC ACAGGTCAAG GCTGAGGTGC AGGAAGACTC AGAAAGCAGT 
GAGGAGTCGG ACAATGAGGA AGCGGCTGCA ACTCCCGCAC AGGTGAAGAC TTCAGTGAAA 
ACCCCTCAGA CCAAAGCCAA CTCTTCTGCC ACCAGAGTGG CTTCAGCCAA AGCAGCAGCA 
CCAGCTCCTG GAAAGGGGGG CCTTGCTGTT GCTCAAGCCA AAGTGGGGTC CCCAGCCAAG 
GTAAAACCAC CAGCGAGAAC CCCCCAGAGT AGCGCCGTCT CTGGGAGGGG CCAGGTGTCT 
GTGCCGGCTG TGGGGAAGGC AGCAGCTGCA GCAGCCCAGG CCCAGCCGGG GCCTGTCAAG 
GGCTCACAGG AGGACTCGGA GAGCAGTGAG GAGGAGTCGG ACAGTGAGGG AGAGGCACCT 
CCTCAGGGGA AGCCCTCAGG AAAGACCCCC CAGGTCAGAA CTGCCTCAGC CCCCACCAAG 
GCGTCCCCCA AGAAAGGGGC TGCCCCAGTA CCCCCTGGGA AAGCAGGACC CCCAGCCACC 
CAGGCCAAGA GGCAGGAGGA GGACTCGGAG AGCAGCAGCG AGGAGGAGTC GGACAGTGAC 
GGGGACGTGC CAGCTGCTGC ACCTCCAGCC CAGAAAGAGG GTAGCTCCAA AACTGCCACA 
AGCAAGACCC TGGCCCCAGC GTCCCTGGAG AAGAAAGCAG AGGAGTCCTC AACCAGCAGT 
GATGAGGACC TGCCATCCAG CCAGGTGATT AAAACCCCTC TGATTTTTGT CGACCCTAAT 
CGTAGTCCAG CTGGTCCAGC TGCTACCCCC GCCCAAGCGC AGGCTACAGG CACCCTGAGG 
AAGGCTCAGG CCTTGGAGAG CACAGCCAGG AGCTCCTCTG AGAGCGAGGA CGAGGACGTG 
ATCCCCGCTA CACAGTGCCC GACTCCTGCC ACTAGGACCA GCGTGGTGAC TGTGCCCACG 
GCACCCCCAA AGGCGGCCCT CAGAGCCAGT GTGGTGGGGG CTCCCAGCAG TGAGGACACA 
AGTCAGGGGT CTGAAGGCAA GAAGCAGGAG GCCTCAGCCA CTCAGGTGAC CAAGAGGAAC 
CTCCCGCTGA CACAGGCTGC CCTGAAGGTT CTTGCCCAGA AAGCCAGTGA GGCCCAATCT 
CCTGCTGCCA AGACCCCGTC TTCAAGTGGG ACTGACAGTG CTGTGGGAAC ACTCTCCACG 
GCAAGTCCCC AGAGCACCCC TACCCAGGTC AGAATGGCCA ACAAGCTCAG AAAGCCTGAG 
GTTCCTACAG CTCAGCAGGC CACAGCCACT CCCTCGGGAC ACCCCACAGC CAAGGCCCCT 
GCAACCTCAG ATGACAGCAA CAGCAGCTCT TCAGGGAGCG AGGACGATGC CAAGGGGTCC 
CAGGCGGCCC CGTCAGCCCA CAGGCCAGGC CCAGCCCCCT CCAGGAGGGA GACCTTGGTG 
GAAGAGACCA CAACAGAGTC CAGTGACGAC GAGGTGGTGG CACCTTCCCA GTCTCTCCTC 
TCAGGTTTTG TGACTCCTGG GCTGACTCCA GCTGGTTCCA AGACTTCAAA GGCCGCTCCC 
AAACCAGACG CCAGCCCCTC TGTTTCCTCT ACTCCGGCCA CCAGAGATGC CCCAGAGGGC 
AAGCAGGAAG TGGAGCCCCA ACAAGCAGCA GGCACCATGT CCCCTAAAAC AGGCAGAAAA 
GAGGCTGGTG CCACACCTCA GAAGCCCGGG AAGGGGCTCG GGAGTCCCCC GGCCTCCATG 
CTGGCGCTGC AGAGCAACAT CACCCAGCGC CTCCTGAGCG AGCCCTGGCC CCTGAGCGAG 
GCGCAGGTGC AGGCCTCGGT GGCGAAGGTG CTCACGGAGC TGCTGGAGCA GGAGAGGAAG 
AAGGCCATGG ACACCGCCAA GGAAGGCAGC CGGAAGGGCC GCCTGGGCCA TAAGCGGAAG 
CTGTCCGAAG ACCAGACAGC CCCCAAGGCC CCCAAGAACA AGAAAAAGAA GCAGCTGGCA 
GCTGCAGATG GTGGGGAGCA TGTCGTCTCC TCAGAAAAGG CCCCCAGGAC TGTCAAAGGG 
AAATCCAAGA AGGACAGAGC AAGTGGTGAC GTCAAGGAGA AGAAGGAGAA GGAGTCTCCC 
GGCTCTCAAG GGGCCAAGGA GAAGCCAGTA GGGGAGCTGG GGACTCCAAA GGGTGACGGG 
GGAGACCACG GCAACCTTAA GATGAAGAAA GAGAAGAAGA AATCTGACAA GAAAAAAAAA 
GACAAGGAGA AAAAGGAAAA GAAGAAGAAA GCAAAAAAGG CCTCAACCAA AGACCCTGGC 
TCACCATCAC AGAAGAAAAA GAAGAGAAAG AAGAAGACAG CAGAGCAAAC CGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_010798510.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010796812.1 Bos taurus Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa05568
Clone ID Related Accession (Same CDS sequence)	XM_002689293.5 , XM_010807517.2 , XM_591291.9 , XM_010798510.2
Accession Version	XM_010798510.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4377bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	treacle protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645112.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010798510.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAGG CCAGGAAGCG GCGAGAGCTC CTTCCCCTGA TCTACCAGCA TCTGCTGCAG 
GCGGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA AATTTTCCTG 
AGTCAGCCTG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACTTC GGAGATTGGC 
CGGAAACGGA AGGCAGAGGA AGATGCGGCC TTGCAGGCCA AGAGGAGCCG TGTGTCAGAC 
CCCATCAGCA GCTCAGAAAG CTCAGAAGAG GAGGAGGAAG AGGAGGAGGC AGAAGCCAAA 
ACCACCAAAC CCACCCCGAG ATTAGCAGCT ACCAACTCCT CAGTCACAGG GACAGTTTCG 
TCTTCAAATG TGAAAGAAAA AGCCAAGGCA AAGACCACGA AAGCCAGCAA GACGGTGAAC 
TCCACGACAC ACCCTGCCCC GGGAAAGGCA GTGGCCCACC TCCTCACTGG GAAGTCACCC 
CAGAAGACAT CGGGGCCCTC GGCAAATGCT GTCTTGGTGT CAGAAACCGA GGAGGAGGGC 
AGCGTGTCAG CCCTCAGAAC CGCCACCACG CCTGGAATGG CATCCGCCGA CCAGGCTGAC 
AGCTCCAGTG AGGATACCAC CAGCTCGAGT GATGAGACGG ACTTGGAGGC GAAAGCCGCA 
GTAAAACCAC TGCAGGTCAG AGCCTCAGCC ACTGCAGTCA AGGAGTCTCC GAGAAAAAGT 
GCAGCCCCGA CTCCAGGGAA GGCAGGGGTT GTGACACCCC AAGTCAGAGG CGGTGCTGTG 
ACCCCAGCCA GCAGGGCCAA GAAGCCAGAA GAGGTCTCAG AGAGCAGTGA AGAGTCCGAC 
GAGAGTGAGG AGGCCGCCCC TGCGGGGACA CCCAGTCAGG TGAAGGCCTT GGAGAAAACC 
GTCCCAATCA GAGTTGCTCC TAGTCCTGCC AAGGGGACCC CTGGGAAAGG GGCCACCCCA 
GCACCCCCTG GGAAGGCAGG GCCCTTAGGG AAGCCAGAGG AGGACTCGGA GAGCAGCAGC 
GAGGAGTCAG ACAGCGAGGA GGAGACACAA GCTGTCAAGA CCCCAGTGCA GGCAAAGCCC 
TCTGGGAAGA TCCCCCAGGT TAAAGCTGCC TCCACACCAA AAATCCAGGT GTTTTTCCCT 
AAGAAAGGGT CCCCTCCAAC ATCCCCTGGC AAGGCAGGGC CTGCAGCCAC CCAAGCCCAG 
CCCAGGAAGC AGGAGGAGGA GGAGAGCAGC AGCAGCAGCA GCAAGGAATC AGACAGCGAG 
GGCGAGGTGC CCACGGCCAT GCAGCAGACT ACAAGCCTGG CTCAGGCAAA GCCCTTGGAG 
AAAAACTCTC AGGTCAGAGC TGCCTCAGCC ACAGGTGCAG GACCCTCGGC AAAGGGCACG 
GTCCCAGCGC TCCCTCAGAA GGCAAGGTCT GTGGCCGCCC AGGATGGGAA GCAGGAGGAC 
TCAGAGAGCA GCAGTGAGGA GGAGTCAGAC AGCGAGGAGG AGATGCAGAA GGCAGGGACC 
TCAGCCCAGG CACAGTCCTC TGGGAAAGCC CTCCAGATCA GACCTGCCTC CGGTCCCACC 
AAGAGGCCCC CTCAGAAGGC CGGGCCTGCA ACCACCCAGG TCAAGGTTGA AAGGGCCGAG 
GAAGACTCGG AGAGCAGTGA GGAGGAATCA GACAGTGAGG ATGAAGCTCC TGTAGCCAAG 
ACTCCAGCCC AGGCAAAATC AGCTGTGAAA ACTTCTCAGA TCAAGGCCTC CCCAAGGAAG 
GGCACCCCCA TTACACCCTC ATCTGCCAGG GTCCCCCCAG TGCAAGTCGG CACACCAGCC 
CCCCGGACAG CAGCAGCAGT GAGTACGCCC ACCTGCGCGT CATCCCCAGC CATAGTCAGG 
CACACCCAGA AGCCAGGGGA GGACTCTTCA AGCAGCGAGG AGTCCGAGAG CGAGGAGGAG 
ACAGCTCCTG CTGCAGCAGG GGGACAGATG AAGTCTATAG GGAAGACCCT CCCAGTGAAA 
GCTGCCTCAC CAGCCACCAA GGGGCCCTCA GGAAAAGGGA CCACCCCAGG GCCCCCTGGG 
AAGGCAGGAC CTGCAGTGGC ACAGGTCAAG GCTGAGGTGC AGGAAGACTC AGAAAGCAGT 
GAGGAGTCGG ACAATGAGGA AGCGGCTGCA ACTCCCGCAC AGGTGAAGAC TTCAGTGAAA 
ACCCCTCAGA CCAAAGCCAA CTCTTCTGCC ACCAGAGTGG CTTCAGCCAA AGCAGCAGCA 
CCAGCTCCTG GAAAGGGGGG CCTTGCTGTT GCTCAAGCCA AAGTGGGGTC CCCAGCCAAG 
GTAAAACCAC CAGCGAGAAC CCCCCAGAGT AGCGCCGTCT CTGGGAGGGG CCAGGTGTCT 
GTGCCGGCTG TGGGGAAGGC AGCAGCTGCA GCAGCCCAGG CCCAGCCGGG GCCTGTCAAG 
GGCTCACAGG AGGACTCGGA GAGCAGTGAG GAGGAGTCGG ACAGTGAGGG AGAGGCACCT 
CCTCAGGGGA AGCCCTCAGG AAAGACCCCC CAGGTCAGAA CTGCCTCAGC CCCCACCAAG 
GCGTCCCCCA AGAAAGGGGC TGCCCCAGTA CCCCCTGGGA AAGCAGGACC CCCAGCCACC 
CAGGCCAAGA GGCAGGAGGA GGACTCGGAG AGCAGCAGCG AGGAGGAGTC GGACAGTGAC 
GGGGACGTGC CAGCTGCTGC ACCTCCAGCC CAGAAAGAGG GTAGCTCCAA AACTGCCACA 
AGCAAGACCC TGGCCCCAGC GTCCCTGGAG AAGAAAGCAG AGGAGTCCTC AACCAGCAGT 
GATGAGGACC TGCCATCCAG CCAGGTGATT AAAACCCCTC TGATTTTTGT CGACCCTAAT 
CGTAGTCCAG CTGGTCCAGC TGCTACCCCC GCCCAAGCGC AGGCTACAGG CACCCTGAGG 
AAGGCTCAGG CCTTGGAGAG CACAGCCAGG AGCTCCTCTG AGAGCGAGGA CGAGGACGTG 
ATCCCCGCTA CACAGTGCCC GACTCCTGCC ACTAGGACCA GCGTGGTGAC TGTGCCCACG 
GCACCCCCAA AGGCGGCCCT CAGAGCCAGT GTGGTGGGGG CTCCCAGCAG TGAGGACACA 
AGTCAGGGGT CTGAAGGCAA GAAGCAGGAG GCCTCAGCCA CTCAGGTGAC CAAGAGGAAC 
CTCCCGCTGA CACAGGCTGC CCTGAAGGTT CTTGCCCAGA AAGCCAGTGA GGCCCAATCT 
CCTGCTGCCA AGACCCCGTC TTCAAGTGGG ACTGACAGTG CTGTGGGAAC ACTCTCCACG 
GCAAGTCCCC AGAGCACCCC TACCCAGGTC AGAATGGCCA ACAAGCTCAG AAAGCCTGAG 
GTTCCTACAG CTCAGCAGGC CACAGCCACT CCCTCGGGAC ACCCCACAGC CAAGGCCCCT 
GCAACCTCAG ATGACAGCAA CAGCAGCTCT TCAGGGAGCG AGGACGATGC CAAGGGGTCC 
CAGGCGGCCC CGTCAGCCCA CAGGCCAGGC CCAGCCCCCT CCAGGAGGGA GACCTTGGTG 
GAAGAGACCA CAACAGAGTC CAGTGACGAC GAGGTGGTGG CACCTTCCCA GTCTCTCCTC 
TCAGGTTTTG TGACTCCTGG GCTGACTCCA GCTGGTTCCA AGACTTCAAA GGCCGCTCCC 
AAACCAGACG CCAGCCCCTC TGTTTCCTCT ACTCCGGCCA CCAGAGATGC CCCAGAGGGC 
AAGCAGGAAG TGGAGCCCCA ACAAGCAGCA GGCACCATGT CCCCTAAAAC AGGCAGAAAA 
GAGGCTGGTG CCACACCTCA GAAGCCCGGG AAGGGGCTCG GGAGTCCCCC GGCCTCCATG 
CTGGCGCTGC AGAGCAACAT CACCCAGCGC CTCCTGAGCG AGCCCTGGCC CCTGAGCGAG 
GCGCAGGTGC AGGCCTCGGT GGCGAAGGTG CTCACGGAGC TGCTGGAGCA GGAGAGGAAG 
AAGGCCATGG ACACCGCCAA GGAAGGCAGC CGGAAGGGCC GCCTGGGCCA TAAGCGGAAG 
CTGTCCGAAG ACCAGACAGC CCCCAAGGCC CCCAAGAACA AGAAAAAGAA GCAGCTGGCA 
GCTGCAGATG GTGGGGAGCA TGTCGTCTCC TCAGAAAAGG CCCCCAGGAC TGTCAAAGGG 
AAATCCAAGA AGGACAGAGC AAGTGGTGAC GTCAAGGAGA AGAAGGAGAA GGAGTCTCCC 
GGCTCTCAAG GGGCCAAGGA GAAGCCAGTA GGGGAGCTGG GGACTCCAAA GGGTGACGGG 
GGAGACCACG GCAACCTTAA GATGAAGAAA GAGAAGAAGA AATCTGACAA GAAAAAAAAA 
GACAAGGAGA AAAAGGAAAA GAAGAAGAAA GCAAAAAAGG CCTCAACCAA AGACCCTGGC 
TCACCATCAC AGAAGAAAAA GAAGAGAAAG AAGAAGACAG CAGAGCAAAC CGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010796812.1
CDS	59..4435
Translation	MAEARKRRELLPLIYQHLLQAGYVRAAREVKEQSGQKIFLSQPVTLLDIYTHWQQTSEIGRKRKAEEDAALQAKRSRVSDPISSSESSEEEEEEEEAEAKTTKPTPRLAATNSSVTGTVSSSNVKEKAKAKTTKASKTVNSTTHPAPGKAVAHLLTGKSPQKTSGPSANAVLVSETEEEGSVSALRTATTPGMASADQADSSSEDTTSSSDETDLEAKAAVKPLQVRASATAVKESPRKSAAPTPGKAGVVTPQVRGGAVTPASRAKKPEEVSESSEESDESEEAAPAGTPSQVKALEKTVPIRVAPSPAKGTPGKGATPAPPGKAGPLGKPEEDSESSSEESDSEEETQAVKTPVQAKPSGKIPQVKAASTPKIQVFFPKKGSPPTSPGKAGPAATQAQPRKQEEEESSSSSSKESDSEGEVPTAMQQTTSLAQAKPLEKNSQVRAASATGAGPSAKGTVPALPQKARSVAAQDGKQEDSESSSEEESDSEEEMQKAGTSAQAQSSGKALQIRPASGPTKRPPQKAGPATTQVKVERAEEDSESSEEESDSEDEAPVAKTPAQAKSAVKTSQIKASPRKGTPITPSSARVPPVQVGTPAPRTAAAVSTPTCASSPAIVRHTQKPGEDSSSSEESESEEETAPAAAGGQMKSIGKTLPVKAASPATKGPSGKGTTPGPPGKAGPAVAQVKAEVQEDSESSEESDNEEAAATPAQVKTSVKTPQTKANSSATRVASAKAAAPAPGKGGLAVAQAKVGSPAKVKPPARTPQSSAVSGRGQVSVPAVGKAAAAAAQAQPGPVKGSQEDSESSEEESDSEGEAPPQGKPSGKTPQVRTASAPTKASPKKGAAPVPPGKAGPPATQAKRQEEDSESSSEEESDSDGDVPAAAPPAQKEGSSKTATSKTLAPASLEKKAEESSTSSDEDLPSSQVIKTPLIFVDPNRSPAGPAATPAQAQATGTLRKAQALESTARSSSESEDEDVIPATQCPTPATRTSVVTVPTAPPKAALRASVVGAPSSEDTSQGSEGKKQEASATQVTKRNLPLTQAALKVLAQKASEAQSPAAKTPSSSGTDSAVGTLSTASPQSTPTQVRMANKLRKPEVPTAQQATATPSGHPTAKAPATSDDSNSSSSGSEDDAKGSQAAPSAHRPGPAPSRRETLVEETTTESSDDEVVAPSQSLLSGFVTPGLTPAGSKTSKAAPKPDASPSVSSTPATRDAPEGKQEVEPQQAAGTMSPKTGRKEAGATPQKPGKGLGSPPASMLALQSNITQRLLSEPWPLSEAQVQASVAKVLTELLEQERKKAMDTAKEGSRKGRLGHKRKLSEDQTAPKAPKNKKKKQLAAADGGEHVVSSEKAPRTVKGKSKKDRASGDVKEKKEKESPGSQGAKEKPVGELGTPKGDGGDHGNLKMKKEKKKSDKKKKDKEKKEKKKKAKKASTKDPGSPSQKKKKRKKKTAEQTV

Target ORF information:

RefSeq Version	XM_010798510.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010798510.2

ORF Insert Sequence:

ATGGCTGAGG CCAGGAAGCG GCGAGAGCTC CTTCCCCTGA TCTACCAGCA TCTGCTGCAG 
GCGGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA AATTTTCCTG 
AGTCAGCCTG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACTTC GGAGATTGGC 
CGGAAACGGA AGGCAGAGGA AGATGCGGCC TTGCAGGCCA AGAGGAGCCG TGTGTCAGAC 
CCCATCAGCA GCTCAGAAAG CTCAGAAGAG GAGGAGGAAG AGGAGGAGGC AGAAGCCAAA 
ACCACCAAAC CCACCCCGAG ATTAGCAGCT ACCAACTCCT CAGTCACAGG GACAGTTTCG 
TCTTCAAATG TGAAAGAAAA AGCCAAGGCA AAGACCACGA AAGCCAGCAA GACGGTGAAC 
TCCACGACAC ACCCTGCCCC GGGAAAGGCA GTGGCCCACC TCCTCACTGG GAAGTCACCC 
CAGAAGACAT CGGGGCCCTC GGCAAATGCT GTCTTGGTGT CAGAAACCGA GGAGGAGGGC 
AGCGTGTCAG CCCTCAGAAC CGCCACCACG CCTGGAATGG CATCCGCCGA CCAGGCTGAC 
AGCTCCAGTG AGGATACCAC CAGCTCGAGT GATGAGACGG ACTTGGAGGC GAAAGCCGCA 
GTAAAACCAC TGCAGGTCAG AGCCTCAGCC ACTGCAGTCA AGGAGTCTCC GAGAAAAAGT 
GCAGCCCCGA CTCCAGGGAA GGCAGGGGTT GTGACACCCC AAGTCAGAGG CGGTGCTGTG 
ACCCCAGCCA GCAGGGCCAA GAAGCCAGAA GAGGTCTCAG AGAGCAGTGA AGAGTCCGAC 
GAGAGTGAGG AGGCCGCCCC TGCGGGGACA CCCAGTCAGG TGAAGGCCTT GGAGAAAACC 
GTCCCAATCA GAGTTGCTCC TAGTCCTGCC AAGGGGACCC CTGGGAAAGG GGCCACCCCA 
GCACCCCCTG GGAAGGCAGG GCCCTTAGGG AAGCCAGAGG AGGACTCGGA GAGCAGCAGC 
GAGGAGTCAG ACAGCGAGGA GGAGACACAA GCTGTCAAGA CCCCAGTGCA GGCAAAGCCC 
TCTGGGAAGA TCCCCCAGGT TAAAGCTGCC TCCACACCAA AAATCCAGGT GTTTTTCCCT 
AAGAAAGGGT CCCCTCCAAC ATCCCCTGGC AAGGCAGGGC CTGCAGCCAC CCAAGCCCAG 
CCCAGGAAGC AGGAGGAGGA GGAGAGCAGC AGCAGCAGCA GCAAGGAATC AGACAGCGAG 
GGCGAGGTGC CCACGGCCAT GCAGCAGACT ACAAGCCTGG CTCAGGCAAA GCCCTTGGAG 
AAAAACTCTC AGGTCAGAGC TGCCTCAGCC ACAGGTGCAG GACCCTCGGC AAAGGGCACG 
GTCCCAGCGC TCCCTCAGAA GGCAAGGTCT GTGGCCGCCC AGGATGGGAA GCAGGAGGAC 
TCAGAGAGCA GCAGTGAGGA GGAGTCAGAC AGCGAGGAGG AGATGCAGAA GGCAGGGACC 
TCAGCCCAGG CACAGTCCTC TGGGAAAGCC CTCCAGATCA GACCTGCCTC CGGTCCCACC 
AAGAGGCCCC CTCAGAAGGC CGGGCCTGCA ACCACCCAGG TCAAGGTTGA AAGGGCCGAG 
GAAGACTCGG AGAGCAGTGA GGAGGAATCA GACAGTGAGG ATGAAGCTCC TGTAGCCAAG 
ACTCCAGCCC AGGCAAAATC AGCTGTGAAA ACTTCTCAGA TCAAGGCCTC CCCAAGGAAG 
GGCACCCCCA TTACACCCTC ATCTGCCAGG GTCCCCCCAG TGCAAGTCGG CACACCAGCC 
CCCCGGACAG CAGCAGCAGT GAGTACGCCC ACCTGCGCGT CATCCCCAGC CATAGTCAGG 
CACACCCAGA AGCCAGGGGA GGACTCTTCA AGCAGCGAGG AGTCCGAGAG CGAGGAGGAG 
ACAGCTCCTG CTGCAGCAGG GGGACAGATG AAGTCTATAG GGAAGACCCT CCCAGTGAAA 
GCTGCCTCAC CAGCCACCAA GGGGCCCTCA GGAAAAGGGA CCACCCCAGG GCCCCCTGGG 
AAGGCAGGAC CTGCAGTGGC ACAGGTCAAG GCTGAGGTGC AGGAAGACTC AGAAAGCAGT 
GAGGAGTCGG ACAATGAGGA AGCGGCTGCA ACTCCCGCAC AGGTGAAGAC TTCAGTGAAA 
ACCCCTCAGA CCAAAGCCAA CTCTTCTGCC ACCAGAGTGG CTTCAGCCAA AGCAGCAGCA 
CCAGCTCCTG GAAAGGGGGG CCTTGCTGTT GCTCAAGCCA AAGTGGGGTC CCCAGCCAAG 
GTAAAACCAC CAGCGAGAAC CCCCCAGAGT AGCGCCGTCT CTGGGAGGGG CCAGGTGTCT 
GTGCCGGCTG TGGGGAAGGC AGCAGCTGCA GCAGCCCAGG CCCAGCCGGG GCCTGTCAAG 
GGCTCACAGG AGGACTCGGA GAGCAGTGAG GAGGAGTCGG ACAGTGAGGG AGAGGCACCT 
CCTCAGGGGA AGCCCTCAGG AAAGACCCCC CAGGTCAGAA CTGCCTCAGC CCCCACCAAG 
GCGTCCCCCA AGAAAGGGGC TGCCCCAGTA CCCCCTGGGA AAGCAGGACC CCCAGCCACC 
CAGGCCAAGA GGCAGGAGGA GGACTCGGAG AGCAGCAGCG AGGAGGAGTC GGACAGTGAC 
GGGGACGTGC CAGCTGCTGC ACCTCCAGCC CAGAAAGAGG GTAGCTCCAA AACTGCCACA 
AGCAAGACCC TGGCCCCAGC GTCCCTGGAG AAGAAAGCAG AGGAGTCCTC AACCAGCAGT 
GATGAGGACC TGCCATCCAG CCAGGTGATT AAAACCCCTC TGATTTTTGT CGACCCTAAT 
CGTAGTCCAG CTGGTCCAGC TGCTACCCCC GCCCAAGCGC AGGCTACAGG CACCCTGAGG 
AAGGCTCAGG CCTTGGAGAG CACAGCCAGG AGCTCCTCTG AGAGCGAGGA CGAGGACGTG 
ATCCCCGCTA CACAGTGCCC GACTCCTGCC ACTAGGACCA GCGTGGTGAC TGTGCCCACG 
GCACCCCCAA AGGCGGCCCT CAGAGCCAGT GTGGTGGGGG CTCCCAGCAG TGAGGACACA 
AGTCAGGGGT CTGAAGGCAA GAAGCAGGAG GCCTCAGCCA CTCAGGTGAC CAAGAGGAAC 
CTCCCGCTGA CACAGGCTGC CCTGAAGGTT CTTGCCCAGA AAGCCAGTGA GGCCCAATCT 
CCTGCTGCCA AGACCCCGTC TTCAAGTGGG ACTGACAGTG CTGTGGGAAC ACTCTCCACG 
GCAAGTCCCC AGAGCACCCC TACCCAGGTC AGAATGGCCA ACAAGCTCAG AAAGCCTGAG 
GTTCCTACAG CTCAGCAGGC CACAGCCACT CCCTCGGGAC ACCCCACAGC CAAGGCCCCT 
GCAACCTCAG ATGACAGCAA CAGCAGCTCT TCAGGGAGCG AGGACGATGC CAAGGGGTCC 
CAGGCGGCCC CGTCAGCCCA CAGGCCAGGC CCAGCCCCCT CCAGGAGGGA GACCTTGGTG 
GAAGAGACCA CAACAGAGTC CAGTGACGAC GAGGTGGTGG CACCTTCCCA GTCTCTCCTC 
TCAGGTTTTG TGACTCCTGG GCTGACTCCA GCTGGTTCCA AGACTTCAAA GGCCGCTCCC 
AAACCAGACG CCAGCCCCTC TGTTTCCTCT ACTCCGGCCA CCAGAGATGC CCCAGAGGGC 
AAGCAGGAAG TGGAGCCCCA ACAAGCAGCA GGCACCATGT CCCCTAAAAC AGGCAGAAAA 
GAGGCTGGTG CCACACCTCA GAAGCCCGGG AAGGGGCTCG GGAGTCCCCC GGCCTCCATG 
CTGGCGCTGC AGAGCAACAT CACCCAGCGC CTCCTGAGCG AGCCCTGGCC CCTGAGCGAG 
GCGCAGGTGC AGGCCTCGGT GGCGAAGGTG CTCACGGAGC TGCTGGAGCA GGAGAGGAAG 
AAGGCCATGG ACACCGCCAA GGAAGGCAGC CGGAAGGGCC GCCTGGGCCA TAAGCGGAAG 
CTGTCCGAAG ACCAGACAGC CCCCAAGGCC CCCAAGAACA AGAAAAAGAA GCAGCTGGCA 
GCTGCAGATG GTGGGGAGCA TGTCGTCTCC TCAGAAAAGG CCCCCAGGAC TGTCAAAGGG 
AAATCCAAGA AGGACAGAGC AAGTGGTGAC GTCAAGGAGA AGAAGGAGAA GGAGTCTCCC 
GGCTCTCAAG GGGCCAAGGA GAAGCCAGTA GGGGAGCTGG GGACTCCAAA GGGTGACGGG 
GGAGACCACG GCAACCTTAA GATGAAGAAA GAGAAGAAGA AATCTGACAA GAAAAAAAAA 
GACAAGGAGA AAAAGGAAAA GAAGAAGAAA GCAAAAAAGG CCTCAACCAA AGACCCTGGC 
TCACCATCAC AGAAGAAAAA GAAGAGAAAG AAGAAGACAG CAGAGCAAAC CGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_005209634.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005209691.1 Bos taurus Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant X6, mRNA.

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CloneID	OBa05570
Clone ID Related Accession (Same CDS sequence)	XM_005209634.3 , XM_005199092.3
Accession Version	XM_005209634.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4056bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	treacle protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104039.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005209634.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAGG CCAGGAAGCG GCGAGAGCTC CTTCCCCTGA TCTACCAGCA TCTGCTGCAG 
GCGGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA AATTTTCCTG 
AGTCAGCCTG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACTTC GGAGATTGGC 
CGGAAACGGA AGGCAGAGGA AGATGCGGCC TTGCAGGCCA AGAGGAGCCG TGTGTCAGAC 
CCCATCAGCA GCTCAGAAAG CTCAGAAGAG GAGGAGGAAG AGGAGGAGGC AGAAGCCAAA 
ACCACCAAAC CCACCCCGAG ATTAGCAGCT ACCAACTCCT CAGTCACAGG GACAGTTTCG 
TCTTCAAATG TGAAAGAAAA AGCCAAGGCA AAGACCACGA AAGCCAGCAA GACGGTGAAC 
TCCACGACAC ACCCTGCCCC GGGAAAGGCA GTGGCCCACC TCCTCACTGG GAAGTCACCC 
CAGAAGACAT CGGGGCCCTC GGCAAATGCT GTCTTGGTGT CAGAAACCGA GGAGGAGGGC 
AGCGTGTCAG CCCTCAGAAC CGCCACCACG CCTGGAATGG CATCCGCCGA CCAGGCTGAC 
AGCTCCAGTG AGGATACCAC CAGCTCGAGT GATGAGACGG ACTTGGAGGC GAAAGCCGCA 
GTAAAACCAC TGCAGGTCAG AGCCTCAGCC ACTGCAGTCA AGGAGTCTCC GAGAAAAAGT 
GCAGCCCCGA CTCCAGGGAA GGCAGGGGTT GTGACACCCC AAGTCAGAGG CGGTGCTGTG 
ACCCCAGCCA GCAGGGCCAA GAAGCCAGAA GAGGTCTCAG AGAGCAGTGA AGAGTCCGAC 
GAGAGTGAGG AGGCCGCCCC TGCGGGGACA CCCAGTCAGG TGAAGGCCTT GGAGAAAACC 
GTCCCAATCA GAGTTGCTCC TAGTCCTGCC AAGGGGACCC CTGGGAAAGG GGCCACCCCA 
GCACCCCCTG GGAAGGCAGG GCCCTTAGGG AAGCCAGAGG AGGACTCGGA GAGCAGCAGC 
GAGGAGTCAG ACAGCGAGGA GGAGACACAA GCTGTCAAGA CCCCAGTGCA GGCAAAGCCC 
TCTGGGAAGA TCCCCCAGGT TAAAGCTGCC TCCACACCAA AAATCCAGGT GTTTTTCCCT 
AAGAAAGGGT CCCCTCCAAC ATCCCCTGGC AAGGCAGGGC CTGCAGCCAC CCAAGCCCAG 
CCCAGGAAGC AGGAGGAGGA GGAGAGCAGC AGCAGCAGCA GCAAGGAATC AGACAGCGAG 
GGCGAGGTGC CCACGGCCAT GCAGCAGACT ACAAGCCTGG CTCAGGCAAA GCCCTTGGAG 
AAAAACTCTC AGGTCAGAGC TGCCTCAGCC ACAGGTGCAG GACCCTCGGC AAAGGGCACG 
GTCCCAGCGC TCCCTCAGAA GGCAAGGTCT GTGGCCGCCC AGGATGGGAA GCAGGAGGAC 
TCAGAGAGCA GCAGTGAGGA GGAGTCAGAC AGCGAGGAGG AGATGCAGAA GGCAGGGACC 
TCAGCCCAGG CACAGTCCTC TGGGAAAGCC CTCCAGATCA GACCTGCCTC CGGTCCCACC 
AAGAGGCCCC CTCAGAAGGC CGGGCCTGCA ACCACCCAGG TCAAGGTTGA AAGGGCCGAG 
GAAGACTCGG AGAGCAGTGA GGAGGAATCA GACAGTGAGG ATGAAGCTCC TGTAGCCAAG 
ACTCCAGCCC AGGCAAAATC AGCTGTGAAA ACTTCTCAGA TCAAGGCCTC CCCAAGGAAG 
GGCACCCCCA TTACACCCTC ATCTGCCAGG GTCCCCCCAG TGCAAGTCGG CACACCAGCC 
CCCCGGACAG CAGCAGCAGT GAGTACGCCC ACCTGCGCGT CATCCCCAGC CATAGTCAGG 
CACACCCAGA AGCCAGGGGA GGACTCTTCA AGCAGCGAGG AGTCCGAGAG CGAGGAGGAG 
ACAGCTCCTG CTGCAGCAGG GGGACAGATG AAGTCTATAG GGAAGACCCT CCCAGTGAAA 
GCTGCCTCAC CAGCCACCAA GGGGCCCTCA GGAAAAGGGA CCACCCCAGG GCCCCCTGGG 
AAGGCAGGAC CTGCAGTGGC ACAGGTCAAG GCTGAGGTGC AGGAAGACTC AGAAAGCAGT 
GAGGAGTCGG ACAATGAGGA AGCGGCTGCA ACTCCCGCAC AGGTGAAGAC TTCAGTGAAA 
ACCCCTCAGA CCAAAGCCAA CTCTTCTGCC ACCAGAGTGG CTTCAGCCAA AGCAGCAGCA 
CCAGCTCCTG GAAAGGGGGG CCTTGCTGTT GCTCAAGCCA AAGTGGGGTC CCCAGCCAAG 
GTAAAACCAC CAGCGAGAAC CCCCCAGAGT AGCGCCGTCT CTGGGAGGGG CCAGGTGTCT 
GTGCCGGCTG TGGGGAAGGC AGCAGCTGCA GCAGCCCAGG CCCAGCCGGG GCCTGTCAAG 
GGCTCACAGG AGGACTCGGA GAGCAGTGAG GAGGAGTCGG ACAGTGAGGG AGAGGCACCT 
CCTCAGGGGA AGCCCTCAGG AAAGACCCCC CAGGTCAGAA CTGCCTCAGC CCCCACCAAG 
GCGTCCCCCA AGAAAGGGGC TGCCCCAGTA CCCCCTGGGA AAGCAGGACC CCCAGCCACC 
CAGGCCAAGA GGCAGGAGGA GGACTCGGAG AGCAGCAGCG AGGAGGAGTC GGACAGTGAC 
GGGGACGTGC CAGCTGCTGC ACCTCCAGCC CAGAAAGAGG GTAGCTCCAA AACTGCCACA 
AGCAAGACCC TGGCCCCAGC GTCCCTGGAG AAGAAAGCAG AGGAGTCCTC AACCAGCAGT 
GATGAGGACC TGCCATCCAG CCAGGTGACC AAGAGGAACC TCCCGCTGAC ACAGGCTGCC 
CTGAAGGTTC TTGCCCAGAA AGCCAGTGAG GCCCAATCTC CTGCTGCCAA GACCCCGTCT 
TCAAGTGGGA CTGACAGTGC TGTGGGAACA CTCTCCACGG CAAGTCCCCA GAGCACCCCT 
ACCCAGGTCA GAATGGCCAA CAAGCTCAGA AAGCCTGAGG TTCCTACAGC TCAGCAGGCC 
ACAGCCACTC CCTCGGGACA CCCCACAGCC AAGGCCCCTG CAACCTCAGA TGACAGCAAC 
AGCAGCTCTT CAGGGAGCGA GGACGATGCC AAGGGGTCCC AGGCGGCCCC GTCAGCCCAC 
AGGCCAGGCC CAGCCCCCTC CAGGAGGGAG ACCTTGGTGG AAGAGACCAC AACAGAGTCC 
AGTGACGACG AGGTGGTGGC ACCTTCCCAG TCTCTCCTCT CAGGTTTTGT GACTCCTGGG 
CTGACTCCAG CTGGTTCCAA GACTTCAAAG GCCGCTCCCA AACCAGACGC CAGCCCCTCT 
GTTTCCTCTA CTCCGGCCAC CAGAGATGCC CCAGAGGGCA AGCAGGAAGT GGAGCCCCAA 
CAAGCAGCAG GCACCATGTC CCCTAAAACA GGCAGAAAAG AGGCTGGTGC CACACCTCAG 
AAGCCCGGGA AGGGGCTCGG GAGTCCCCCG GCCTCCATGC TGGCGCTGCA GAGCAACATC 
ACCCAGCGCC TCCTGAGCGA GCCCTGGCCC CTGAGCGAGG CGCAGGTGCA GGCCTCGGTG 
GCGAAGGTGC TCACGGAGCT GCTGGAGCAG GAGAGGAAGA AGGCCATGGA CACCGCCAAG 
GAAGGCAGCC GGAAGGGCCG CCTGGGCCAT AAGCGGAAGC TGTCCGAAGA CCAGACAGCC 
CCCAAGGCCC CCAAGAACAA GAAAAAGAAG CAGCTGGCAG CTGCAGATGG TGGGGAGCAT 
GTCGTCTCCT CAGAAAAGGC CCCCAGGACT GTCAAAGGGA AATCCAAGAA GGACAGAGCA 
AGTGGTGACG TCAAGGAGAA GAAGGAGAAG GAGTCTCCCG GCTCTCAAGG GGCCAAGGAG 
AAGCCAGTAG GGGAGCTGGG GACTCCAAAG GGTGACGGGG GAGACCACGG CAACCTTAAG 
ATGAAGAAAG AGAAGAAGAA ATCTGACAAG AAAAAAAAAG ACAAGGAGAA AAAGGAAAAG 
AAGAAGAAAG CAAAAAAGGC CTCAACCAAA GACCCTGGCT CACCATCACA GAAGAAAAAG 
AAGAGAAAGA AGAAGACAGC AGAGCAAACC GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005209691.1
CDS	59..4114
Translation	MAEARKRRELLPLIYQHLLQAGYVRAAREVKEQSGQKIFLSQPVTLLDIYTHWQQTSEIGRKRKAEEDAALQAKRSRVSDPISSSESSEEEEEEEEAEAKTTKPTPRLAATNSSVTGTVSSSNVKEKAKAKTTKASKTVNSTTHPAPGKAVAHLLTGKSPQKTSGPSANAVLVSETEEEGSVSALRTATTPGMASADQADSSSEDTTSSSDETDLEAKAAVKPLQVRASATAVKESPRKSAAPTPGKAGVVTPQVRGGAVTPASRAKKPEEVSESSEESDESEEAAPAGTPSQVKALEKTVPIRVAPSPAKGTPGKGATPAPPGKAGPLGKPEEDSESSSEESDSEEETQAVKTPVQAKPSGKIPQVKAASTPKIQVFFPKKGSPPTSPGKAGPAATQAQPRKQEEEESSSSSSKESDSEGEVPTAMQQTTSLAQAKPLEKNSQVRAASATGAGPSAKGTVPALPQKARSVAAQDGKQEDSESSSEEESDSEEEMQKAGTSAQAQSSGKALQIRPASGPTKRPPQKAGPATTQVKVERAEEDSESSEEESDSEDEAPVAKTPAQAKSAVKTSQIKASPRKGTPITPSSARVPPVQVGTPAPRTAAAVSTPTCASSPAIVRHTQKPGEDSSSSEESESEEETAPAAAGGQMKSIGKTLPVKAASPATKGPSGKGTTPGPPGKAGPAVAQVKAEVQEDSESSEESDNEEAAATPAQVKTSVKTPQTKANSSATRVASAKAAAPAPGKGGLAVAQAKVGSPAKVKPPARTPQSSAVSGRGQVSVPAVGKAAAAAAQAQPGPVKGSQEDSESSEEESDSEGEAPPQGKPSGKTPQVRTASAPTKASPKKGAAPVPPGKAGPPATQAKRQEEDSESSSEEESDSDGDVPAAAPPAQKEGSSKTATSKTLAPASLEKKAEESSTSSDEDLPSSQVTKRNLPLTQAALKVLAQKASEAQSPAAKTPSSSGTDSAVGTLSTASPQSTPTQVRMANKLRKPEVPTAQQATATPSGHPTAKAPATSDDSNSSSSGSEDDAKGSQAAPSAHRPGPAPSRRETLVEETTTESSDDEVVAPSQSLLSGFVTPGLTPAGSKTSKAAPKPDASPSVSSTPATRDAPEGKQEVEPQQAAGTMSPKTGRKEAGATPQKPGKGLGSPPASMLALQSNITQRLLSEPWPLSEAQVQASVAKVLTELLEQERKKAMDTAKEGSRKGRLGHKRKLSEDQTAPKAPKNKKKKQLAAADGGEHVVSSEKAPRTVKGKSKKDRASGDVKEKKEKESPGSQGAKEKPVGELGTPKGDGGDHGNLKMKKEKKKSDKKKKDKEKKEKKKKAKKASTKDPGSPSQKKKKRKKKTAEQTV

Target ORF information:

RefSeq Version	XM_005209634.3
Organism	Bos taurus(cattle)
Definition	Bos taurus Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005209634.3

ORF Insert Sequence:

ATGGCTGAGG CCAGGAAGCG GCGAGAGCTC CTTCCCCTGA TCTACCAGCA TCTGCTGCAG 
GCGGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA AATTTTCCTG 
AGTCAGCCTG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACTTC GGAGATTGGC 
CGGAAACGGA AGGCAGAGGA AGATGCGGCC TTGCAGGCCA AGAGGAGCCG TGTGTCAGAC 
CCCATCAGCA GCTCAGAAAG CTCAGAAGAG GAGGAGGAAG AGGAGGAGGC AGAAGCCAAA 
ACCACCAAAC CCACCCCGAG ATTAGCAGCT ACCAACTCCT CAGTCACAGG GACAGTTTCG 
TCTTCAAATG TGAAAGAAAA AGCCAAGGCA AAGACCACGA AAGCCAGCAA GACGGTGAAC 
TCCACGACAC ACCCTGCCCC GGGAAAGGCA GTGGCCCACC TCCTCACTGG GAAGTCACCC 
CAGAAGACAT CGGGGCCCTC GGCAAATGCT GTCTTGGTGT CAGAAACCGA GGAGGAGGGC 
AGCGTGTCAG CCCTCAGAAC CGCCACCACG CCTGGAATGG CATCCGCCGA CCAGGCTGAC 
AGCTCCAGTG AGGATACCAC CAGCTCGAGT GATGAGACGG ACTTGGAGGC GAAAGCCGCA 
GTAAAACCAC TGCAGGTCAG AGCCTCAGCC ACTGCAGTCA AGGAGTCTCC GAGAAAAAGT 
GCAGCCCCGA CTCCAGGGAA GGCAGGGGTT GTGACACCCC AAGTCAGAGG CGGTGCTGTG 
ACCCCAGCCA GCAGGGCCAA GAAGCCAGAA GAGGTCTCAG AGAGCAGTGA AGAGTCCGAC 
GAGAGTGAGG AGGCCGCCCC TGCGGGGACA CCCAGTCAGG TGAAGGCCTT GGAGAAAACC 
GTCCCAATCA GAGTTGCTCC TAGTCCTGCC AAGGGGACCC CTGGGAAAGG GGCCACCCCA 
GCACCCCCTG GGAAGGCAGG GCCCTTAGGG AAGCCAGAGG AGGACTCGGA GAGCAGCAGC 
GAGGAGTCAG ACAGCGAGGA GGAGACACAA GCTGTCAAGA CCCCAGTGCA GGCAAAGCCC 
TCTGGGAAGA TCCCCCAGGT TAAAGCTGCC TCCACACCAA AAATCCAGGT GTTTTTCCCT 
AAGAAAGGGT CCCCTCCAAC ATCCCCTGGC AAGGCAGGGC CTGCAGCCAC CCAAGCCCAG 
CCCAGGAAGC AGGAGGAGGA GGAGAGCAGC AGCAGCAGCA GCAAGGAATC AGACAGCGAG 
GGCGAGGTGC CCACGGCCAT GCAGCAGACT ACAAGCCTGG CTCAGGCAAA GCCCTTGGAG 
AAAAACTCTC AGGTCAGAGC TGCCTCAGCC ACAGGTGCAG GACCCTCGGC AAAGGGCACG 
GTCCCAGCGC TCCCTCAGAA GGCAAGGTCT GTGGCCGCCC AGGATGGGAA GCAGGAGGAC 
TCAGAGAGCA GCAGTGAGGA GGAGTCAGAC AGCGAGGAGG AGATGCAGAA GGCAGGGACC 
TCAGCCCAGG CACAGTCCTC TGGGAAAGCC CTCCAGATCA GACCTGCCTC CGGTCCCACC 
AAGAGGCCCC CTCAGAAGGC CGGGCCTGCA ACCACCCAGG TCAAGGTTGA AAGGGCCGAG 
GAAGACTCGG AGAGCAGTGA GGAGGAATCA GACAGTGAGG ATGAAGCTCC TGTAGCCAAG 
ACTCCAGCCC AGGCAAAATC AGCTGTGAAA ACTTCTCAGA TCAAGGCCTC CCCAAGGAAG 
GGCACCCCCA TTACACCCTC ATCTGCCAGG GTCCCCCCAG TGCAAGTCGG CACACCAGCC 
CCCCGGACAG CAGCAGCAGT GAGTACGCCC ACCTGCGCGT CATCCCCAGC CATAGTCAGG 
CACACCCAGA AGCCAGGGGA GGACTCTTCA AGCAGCGAGG AGTCCGAGAG CGAGGAGGAG 
ACAGCTCCTG CTGCAGCAGG GGGACAGATG AAGTCTATAG GGAAGACCCT CCCAGTGAAA 
GCTGCCTCAC CAGCCACCAA GGGGCCCTCA GGAAAAGGGA CCACCCCAGG GCCCCCTGGG 
AAGGCAGGAC CTGCAGTGGC ACAGGTCAAG GCTGAGGTGC AGGAAGACTC AGAAAGCAGT 
GAGGAGTCGG ACAATGAGGA AGCGGCTGCA ACTCCCGCAC AGGTGAAGAC TTCAGTGAAA 
ACCCCTCAGA CCAAAGCCAA CTCTTCTGCC ACCAGAGTGG CTTCAGCCAA AGCAGCAGCA 
CCAGCTCCTG GAAAGGGGGG CCTTGCTGTT GCTCAAGCCA AAGTGGGGTC CCCAGCCAAG 
GTAAAACCAC CAGCGAGAAC CCCCCAGAGT AGCGCCGTCT CTGGGAGGGG CCAGGTGTCT 
GTGCCGGCTG TGGGGAAGGC AGCAGCTGCA GCAGCCCAGG CCCAGCCGGG GCCTGTCAAG 
GGCTCACAGG AGGACTCGGA GAGCAGTGAG GAGGAGTCGG ACAGTGAGGG AGAGGCACCT 
CCTCAGGGGA AGCCCTCAGG AAAGACCCCC CAGGTCAGAA CTGCCTCAGC CCCCACCAAG 
GCGTCCCCCA AGAAAGGGGC TGCCCCAGTA CCCCCTGGGA AAGCAGGACC CCCAGCCACC 
CAGGCCAAGA GGCAGGAGGA GGACTCGGAG AGCAGCAGCG AGGAGGAGTC GGACAGTGAC 
GGGGACGTGC CAGCTGCTGC ACCTCCAGCC CAGAAAGAGG GTAGCTCCAA AACTGCCACA 
AGCAAGACCC TGGCCCCAGC GTCCCTGGAG AAGAAAGCAG AGGAGTCCTC AACCAGCAGT 
GATGAGGACC TGCCATCCAG CCAGGTGACC AAGAGGAACC TCCCGCTGAC ACAGGCTGCC 
CTGAAGGTTC TTGCCCAGAA AGCCAGTGAG GCCCAATCTC CTGCTGCCAA GACCCCGTCT 
TCAAGTGGGA CTGACAGTGC TGTGGGAACA CTCTCCACGG CAAGTCCCCA GAGCACCCCT 
ACCCAGGTCA GAATGGCCAA CAAGCTCAGA AAGCCTGAGG TTCCTACAGC TCAGCAGGCC 
ACAGCCACTC CCTCGGGACA CCCCACAGCC AAGGCCCCTG CAACCTCAGA TGACAGCAAC 
AGCAGCTCTT CAGGGAGCGA GGACGATGCC AAGGGGTCCC AGGCGGCCCC GTCAGCCCAC 
AGGCCAGGCC CAGCCCCCTC CAGGAGGGAG ACCTTGGTGG AAGAGACCAC AACAGAGTCC 
AGTGACGACG AGGTGGTGGC ACCTTCCCAG TCTCTCCTCT CAGGTTTTGT GACTCCTGGG 
CTGACTCCAG CTGGTTCCAA GACTTCAAAG GCCGCTCCCA AACCAGACGC CAGCCCCTCT 
GTTTCCTCTA CTCCGGCCAC CAGAGATGCC CCAGAGGGCA AGCAGGAAGT GGAGCCCCAA 
CAAGCAGCAG GCACCATGTC CCCTAAAACA GGCAGAAAAG AGGCTGGTGC CACACCTCAG 
AAGCCCGGGA AGGGGCTCGG GAGTCCCCCG GCCTCCATGC TGGCGCTGCA GAGCAACATC 
ACCCAGCGCC TCCTGAGCGA GCCCTGGCCC CTGAGCGAGG CGCAGGTGCA GGCCTCGGTG 
GCGAAGGTGC TCACGGAGCT GCTGGAGCAG GAGAGGAAGA AGGCCATGGA CACCGCCAAG 
GAAGGCAGCC GGAAGGGCCG CCTGGGCCAT AAGCGGAAGC TGTCCGAAGA CCAGACAGCC 
CCCAAGGCCC CCAAGAACAA GAAAAAGAAG CAGCTGGCAG CTGCAGATGG TGGGGAGCAT 
GTCGTCTCCT CAGAAAAGGC CCCCAGGACT GTCAAAGGGA AATCCAAGAA GGACAGAGCA 
AGTGGTGACG TCAAGGAGAA GAAGGAGAAG GAGTCTCCCG GCTCTCAAGG GGCCAAGGAG 
AAGCCAGTAG GGGAGCTGGG GACTCCAAAG GGTGACGGGG GAGACCACGG CAACCTTAAG 
ATGAAGAAAG AGAAGAAGAA ATCTGACAAG AAAAAAAAAG ACAAGGAGAA AAAGGAAAAG 
AAGAAGAAAG CAAAAAAGGC CTCAACCAAA GACCCTGGCT CACCATCACA GAAGAAAAAG 
AAGAGAAAGA AGAAGACAGC AGAGCAAACC GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_005199092.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005199149.1 Bos taurus Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant X6, mRNA.

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CloneID	OBa05570
Clone ID Related Accession (Same CDS sequence)	XM_005209634.3 , XM_005199092.3
Accession Version	XM_005199092.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4056bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	treacle protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645112.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005199092.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAGG CCAGGAAGCG GCGAGAGCTC CTTCCCCTGA TCTACCAGCA TCTGCTGCAG 
GCGGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA AATTTTCCTG 
AGTCAGCCTG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACTTC GGAGATTGGC 
CGGAAACGGA AGGCAGAGGA AGATGCGGCC TTGCAGGCCA AGAGGAGCCG TGTGTCAGAC 
CCCATCAGCA GCTCAGAAAG CTCAGAAGAG GAGGAGGAAG AGGAGGAGGC AGAAGCCAAA 
ACCACCAAAC CCACCCCGAG ATTAGCAGCT ACCAACTCCT CAGTCACAGG GACAGTTTCG 
TCTTCAAATG TGAAAGAAAA AGCCAAGGCA AAGACCACGA AAGCCAGCAA GACGGTGAAC 
TCCACGACAC ACCCTGCCCC GGGAAAGGCA GTGGCCCACC TCCTCACTGG GAAGTCACCC 
CAGAAGACAT CGGGGCCCTC GGCAAATGCT GTCTTGGTGT CAGAAACCGA GGAGGAGGGC 
AGCGTGTCAG CCCTCAGAAC CGCCACCACG CCTGGAATGG CATCCGCCGA CCAGGCTGAC 
AGCTCCAGTG AGGATACCAC CAGCTCGAGT GATGAGACGG ACTTGGAGGC GAAAGCCGCA 
GTAAAACCAC TGCAGGTCAG AGCCTCAGCC ACTGCAGTCA AGGAGTCTCC GAGAAAAAGT 
GCAGCCCCGA CTCCAGGGAA GGCAGGGGTT GTGACACCCC AAGTCAGAGG CGGTGCTGTG 
ACCCCAGCCA GCAGGGCCAA GAAGCCAGAA GAGGTCTCAG AGAGCAGTGA AGAGTCCGAC 
GAGAGTGAGG AGGCCGCCCC TGCGGGGACA CCCAGTCAGG TGAAGGCCTT GGAGAAAACC 
GTCCCAATCA GAGTTGCTCC TAGTCCTGCC AAGGGGACCC CTGGGAAAGG GGCCACCCCA 
GCACCCCCTG GGAAGGCAGG GCCCTTAGGG AAGCCAGAGG AGGACTCGGA GAGCAGCAGC 
GAGGAGTCAG ACAGCGAGGA GGAGACACAA GCTGTCAAGA CCCCAGTGCA GGCAAAGCCC 
TCTGGGAAGA TCCCCCAGGT TAAAGCTGCC TCCACACCAA AAATCCAGGT GTTTTTCCCT 
AAGAAAGGGT CCCCTCCAAC ATCCCCTGGC AAGGCAGGGC CTGCAGCCAC CCAAGCCCAG 
CCCAGGAAGC AGGAGGAGGA GGAGAGCAGC AGCAGCAGCA GCAAGGAATC AGACAGCGAG 
GGCGAGGTGC CCACGGCCAT GCAGCAGACT ACAAGCCTGG CTCAGGCAAA GCCCTTGGAG 
AAAAACTCTC AGGTCAGAGC TGCCTCAGCC ACAGGTGCAG GACCCTCGGC AAAGGGCACG 
GTCCCAGCGC TCCCTCAGAA GGCAAGGTCT GTGGCCGCCC AGGATGGGAA GCAGGAGGAC 
TCAGAGAGCA GCAGTGAGGA GGAGTCAGAC AGCGAGGAGG AGATGCAGAA GGCAGGGACC 
TCAGCCCAGG CACAGTCCTC TGGGAAAGCC CTCCAGATCA GACCTGCCTC CGGTCCCACC 
AAGAGGCCCC CTCAGAAGGC CGGGCCTGCA ACCACCCAGG TCAAGGTTGA AAGGGCCGAG 
GAAGACTCGG AGAGCAGTGA GGAGGAATCA GACAGTGAGG ATGAAGCTCC TGTAGCCAAG 
ACTCCAGCCC AGGCAAAATC AGCTGTGAAA ACTTCTCAGA TCAAGGCCTC CCCAAGGAAG 
GGCACCCCCA TTACACCCTC ATCTGCCAGG GTCCCCCCAG TGCAAGTCGG CACACCAGCC 
CCCCGGACAG CAGCAGCAGT GAGTACGCCC ACCTGCGCGT CATCCCCAGC CATAGTCAGG 
CACACCCAGA AGCCAGGGGA GGACTCTTCA AGCAGCGAGG AGTCCGAGAG CGAGGAGGAG 
ACAGCTCCTG CTGCAGCAGG GGGACAGATG AAGTCTATAG GGAAGACCCT CCCAGTGAAA 
GCTGCCTCAC CAGCCACCAA GGGGCCCTCA GGAAAAGGGA CCACCCCAGG GCCCCCTGGG 
AAGGCAGGAC CTGCAGTGGC ACAGGTCAAG GCTGAGGTGC AGGAAGACTC AGAAAGCAGT 
GAGGAGTCGG ACAATGAGGA AGCGGCTGCA ACTCCCGCAC AGGTGAAGAC TTCAGTGAAA 
ACCCCTCAGA CCAAAGCCAA CTCTTCTGCC ACCAGAGTGG CTTCAGCCAA AGCAGCAGCA 
CCAGCTCCTG GAAAGGGGGG CCTTGCTGTT GCTCAAGCCA AAGTGGGGTC CCCAGCCAAG 
GTAAAACCAC CAGCGAGAAC CCCCCAGAGT AGCGCCGTCT CTGGGAGGGG CCAGGTGTCT 
GTGCCGGCTG TGGGGAAGGC AGCAGCTGCA GCAGCCCAGG CCCAGCCGGG GCCTGTCAAG 
GGCTCACAGG AGGACTCGGA GAGCAGTGAG GAGGAGTCGG ACAGTGAGGG AGAGGCACCT 
CCTCAGGGGA AGCCCTCAGG AAAGACCCCC CAGGTCAGAA CTGCCTCAGC CCCCACCAAG 
GCGTCCCCCA AGAAAGGGGC TGCCCCAGTA CCCCCTGGGA AAGCAGGACC CCCAGCCACC 
CAGGCCAAGA GGCAGGAGGA GGACTCGGAG AGCAGCAGCG AGGAGGAGTC GGACAGTGAC 
GGGGACGTGC CAGCTGCTGC ACCTCCAGCC CAGAAAGAGG GTAGCTCCAA AACTGCCACA 
AGCAAGACCC TGGCCCCAGC GTCCCTGGAG AAGAAAGCAG AGGAGTCCTC AACCAGCAGT 
GATGAGGACC TGCCATCCAG CCAGGTGACC AAGAGGAACC TCCCGCTGAC ACAGGCTGCC 
CTGAAGGTTC TTGCCCAGAA AGCCAGTGAG GCCCAATCTC CTGCTGCCAA GACCCCGTCT 
TCAAGTGGGA CTGACAGTGC TGTGGGAACA CTCTCCACGG CAAGTCCCCA GAGCACCCCT 
ACCCAGGTCA GAATGGCCAA CAAGCTCAGA AAGCCTGAGG TTCCTACAGC TCAGCAGGCC 
ACAGCCACTC CCTCGGGACA CCCCACAGCC AAGGCCCCTG CAACCTCAGA TGACAGCAAC 
AGCAGCTCTT CAGGGAGCGA GGACGATGCC AAGGGGTCCC AGGCGGCCCC GTCAGCCCAC 
AGGCCAGGCC CAGCCCCCTC CAGGAGGGAG ACCTTGGTGG AAGAGACCAC AACAGAGTCC 
AGTGACGACG AGGTGGTGGC ACCTTCCCAG TCTCTCCTCT CAGGTTTTGT GACTCCTGGG 
CTGACTCCAG CTGGTTCCAA GACTTCAAAG GCCGCTCCCA AACCAGACGC CAGCCCCTCT 
GTTTCCTCTA CTCCGGCCAC CAGAGATGCC CCAGAGGGCA AGCAGGAAGT GGAGCCCCAA 
CAAGCAGCAG GCACCATGTC CCCTAAAACA GGCAGAAAAG AGGCTGGTGC CACACCTCAG 
AAGCCCGGGA AGGGGCTCGG GAGTCCCCCG GCCTCCATGC TGGCGCTGCA GAGCAACATC 
ACCCAGCGCC TCCTGAGCGA GCCCTGGCCC CTGAGCGAGG CGCAGGTGCA GGCCTCGGTG 
GCGAAGGTGC TCACGGAGCT GCTGGAGCAG GAGAGGAAGA AGGCCATGGA CACCGCCAAG 
GAAGGCAGCC GGAAGGGCCG CCTGGGCCAT AAGCGGAAGC TGTCCGAAGA CCAGACAGCC 
CCCAAGGCCC CCAAGAACAA GAAAAAGAAG CAGCTGGCAG CTGCAGATGG TGGGGAGCAT 
GTCGTCTCCT CAGAAAAGGC CCCCAGGACT GTCAAAGGGA AATCCAAGAA GGACAGAGCA 
AGTGGTGACG TCAAGGAGAA GAAGGAGAAG GAGTCTCCCG GCTCTCAAGG GGCCAAGGAG 
AAGCCAGTAG GGGAGCTGGG GACTCCAAAG GGTGACGGGG GAGACCACGG CAACCTTAAG 
ATGAAGAAAG AGAAGAAGAA ATCTGACAAG AAAAAAAAAG ACAAGGAGAA AAAGGAAAAG 
AAGAAGAAAG CAAAAAAGGC CTCAACCAAA GACCCTGGCT CACCATCACA GAAGAAAAAG 
AAGAGAAAGA AGAAGACAGC AGAGCAAACC GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005199149.1
CDS	59..4114
Translation	MAEARKRRELLPLIYQHLLQAGYVRAAREVKEQSGQKIFLSQPVTLLDIYTHWQQTSEIGRKRKAEEDAALQAKRSRVSDPISSSESSEEEEEEEEAEAKTTKPTPRLAATNSSVTGTVSSSNVKEKAKAKTTKASKTVNSTTHPAPGKAVAHLLTGKSPQKTSGPSANAVLVSETEEEGSVSALRTATTPGMASADQADSSSEDTTSSSDETDLEAKAAVKPLQVRASATAVKESPRKSAAPTPGKAGVVTPQVRGGAVTPASRAKKPEEVSESSEESDESEEAAPAGTPSQVKALEKTVPIRVAPSPAKGTPGKGATPAPPGKAGPLGKPEEDSESSSEESDSEEETQAVKTPVQAKPSGKIPQVKAASTPKIQVFFPKKGSPPTSPGKAGPAATQAQPRKQEEEESSSSSSKESDSEGEVPTAMQQTTSLAQAKPLEKNSQVRAASATGAGPSAKGTVPALPQKARSVAAQDGKQEDSESSSEEESDSEEEMQKAGTSAQAQSSGKALQIRPASGPTKRPPQKAGPATTQVKVERAEEDSESSEEESDSEDEAPVAKTPAQAKSAVKTSQIKASPRKGTPITPSSARVPPVQVGTPAPRTAAAVSTPTCASSPAIVRHTQKPGEDSSSSEESESEEETAPAAAGGQMKSIGKTLPVKAASPATKGPSGKGTTPGPPGKAGPAVAQVKAEVQEDSESSEESDNEEAAATPAQVKTSVKTPQTKANSSATRVASAKAAAPAPGKGGLAVAQAKVGSPAKVKPPARTPQSSAVSGRGQVSVPAVGKAAAAAAQAQPGPVKGSQEDSESSEEESDSEGEAPPQGKPSGKTPQVRTASAPTKASPKKGAAPVPPGKAGPPATQAKRQEEDSESSSEEESDSDGDVPAAAPPAQKEGSSKTATSKTLAPASLEKKAEESSTSSDEDLPSSQVTKRNLPLTQAALKVLAQKASEAQSPAAKTPSSSGTDSAVGTLSTASPQSTPTQVRMANKLRKPEVPTAQQATATPSGHPTAKAPATSDDSNSSSSGSEDDAKGSQAAPSAHRPGPAPSRRETLVEETTTESSDDEVVAPSQSLLSGFVTPGLTPAGSKTSKAAPKPDASPSVSSTPATRDAPEGKQEVEPQQAAGTMSPKTGRKEAGATPQKPGKGLGSPPASMLALQSNITQRLLSEPWPLSEAQVQASVAKVLTELLEQERKKAMDTAKEGSRKGRLGHKRKLSEDQTAPKAPKNKKKKQLAAADGGEHVVSSEKAPRTVKGKSKKDRASGDVKEKKEKESPGSQGAKEKPVGELGTPKGDGGDHGNLKMKKEKKKSDKKKKDKEKKEKKKKAKKASTKDPGSPSQKKKKRKKKTAEQTV

Target ORF information:

RefSeq Version	XM_005199092.3
Organism	Bos taurus(cattle)
Definition	Bos taurus Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005199092.3

ORF Insert Sequence:

ATGGCTGAGG CCAGGAAGCG GCGAGAGCTC CTTCCCCTGA TCTACCAGCA TCTGCTGCAG 
GCGGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA AATTTTCCTG 
AGTCAGCCTG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACTTC GGAGATTGGC 
CGGAAACGGA AGGCAGAGGA AGATGCGGCC TTGCAGGCCA AGAGGAGCCG TGTGTCAGAC 
CCCATCAGCA GCTCAGAAAG CTCAGAAGAG GAGGAGGAAG AGGAGGAGGC AGAAGCCAAA 
ACCACCAAAC CCACCCCGAG ATTAGCAGCT ACCAACTCCT CAGTCACAGG GACAGTTTCG 
TCTTCAAATG TGAAAGAAAA AGCCAAGGCA AAGACCACGA AAGCCAGCAA GACGGTGAAC 
TCCACGACAC ACCCTGCCCC GGGAAAGGCA GTGGCCCACC TCCTCACTGG GAAGTCACCC 
CAGAAGACAT CGGGGCCCTC GGCAAATGCT GTCTTGGTGT CAGAAACCGA GGAGGAGGGC 
AGCGTGTCAG CCCTCAGAAC CGCCACCACG CCTGGAATGG CATCCGCCGA CCAGGCTGAC 
AGCTCCAGTG AGGATACCAC CAGCTCGAGT GATGAGACGG ACTTGGAGGC GAAAGCCGCA 
GTAAAACCAC TGCAGGTCAG AGCCTCAGCC ACTGCAGTCA AGGAGTCTCC GAGAAAAAGT 
GCAGCCCCGA CTCCAGGGAA GGCAGGGGTT GTGACACCCC AAGTCAGAGG CGGTGCTGTG 
ACCCCAGCCA GCAGGGCCAA GAAGCCAGAA GAGGTCTCAG AGAGCAGTGA AGAGTCCGAC 
GAGAGTGAGG AGGCCGCCCC TGCGGGGACA CCCAGTCAGG TGAAGGCCTT GGAGAAAACC 
GTCCCAATCA GAGTTGCTCC TAGTCCTGCC AAGGGGACCC CTGGGAAAGG GGCCACCCCA 
GCACCCCCTG GGAAGGCAGG GCCCTTAGGG AAGCCAGAGG AGGACTCGGA GAGCAGCAGC 
GAGGAGTCAG ACAGCGAGGA GGAGACACAA GCTGTCAAGA CCCCAGTGCA GGCAAAGCCC 
TCTGGGAAGA TCCCCCAGGT TAAAGCTGCC TCCACACCAA AAATCCAGGT GTTTTTCCCT 
AAGAAAGGGT CCCCTCCAAC ATCCCCTGGC AAGGCAGGGC CTGCAGCCAC CCAAGCCCAG 
CCCAGGAAGC AGGAGGAGGA GGAGAGCAGC AGCAGCAGCA GCAAGGAATC AGACAGCGAG 
GGCGAGGTGC CCACGGCCAT GCAGCAGACT ACAAGCCTGG CTCAGGCAAA GCCCTTGGAG 
AAAAACTCTC AGGTCAGAGC TGCCTCAGCC ACAGGTGCAG GACCCTCGGC AAAGGGCACG 
GTCCCAGCGC TCCCTCAGAA GGCAAGGTCT GTGGCCGCCC AGGATGGGAA GCAGGAGGAC 
TCAGAGAGCA GCAGTGAGGA GGAGTCAGAC AGCGAGGAGG AGATGCAGAA GGCAGGGACC 
TCAGCCCAGG CACAGTCCTC TGGGAAAGCC CTCCAGATCA GACCTGCCTC CGGTCCCACC 
AAGAGGCCCC CTCAGAAGGC CGGGCCTGCA ACCACCCAGG TCAAGGTTGA AAGGGCCGAG 
GAAGACTCGG AGAGCAGTGA GGAGGAATCA GACAGTGAGG ATGAAGCTCC TGTAGCCAAG 
ACTCCAGCCC AGGCAAAATC AGCTGTGAAA ACTTCTCAGA TCAAGGCCTC CCCAAGGAAG 
GGCACCCCCA TTACACCCTC ATCTGCCAGG GTCCCCCCAG TGCAAGTCGG CACACCAGCC 
CCCCGGACAG CAGCAGCAGT GAGTACGCCC ACCTGCGCGT CATCCCCAGC CATAGTCAGG 
CACACCCAGA AGCCAGGGGA GGACTCTTCA AGCAGCGAGG AGTCCGAGAG CGAGGAGGAG 
ACAGCTCCTG CTGCAGCAGG GGGACAGATG AAGTCTATAG GGAAGACCCT CCCAGTGAAA 
GCTGCCTCAC CAGCCACCAA GGGGCCCTCA GGAAAAGGGA CCACCCCAGG GCCCCCTGGG 
AAGGCAGGAC CTGCAGTGGC ACAGGTCAAG GCTGAGGTGC AGGAAGACTC AGAAAGCAGT 
GAGGAGTCGG ACAATGAGGA AGCGGCTGCA ACTCCCGCAC AGGTGAAGAC TTCAGTGAAA 
ACCCCTCAGA CCAAAGCCAA CTCTTCTGCC ACCAGAGTGG CTTCAGCCAA AGCAGCAGCA 
CCAGCTCCTG GAAAGGGGGG CCTTGCTGTT GCTCAAGCCA AAGTGGGGTC CCCAGCCAAG 
GTAAAACCAC CAGCGAGAAC CCCCCAGAGT AGCGCCGTCT CTGGGAGGGG CCAGGTGTCT 
GTGCCGGCTG TGGGGAAGGC AGCAGCTGCA GCAGCCCAGG CCCAGCCGGG GCCTGTCAAG 
GGCTCACAGG AGGACTCGGA GAGCAGTGAG GAGGAGTCGG ACAGTGAGGG AGAGGCACCT 
CCTCAGGGGA AGCCCTCAGG AAAGACCCCC CAGGTCAGAA CTGCCTCAGC CCCCACCAAG 
GCGTCCCCCA AGAAAGGGGC TGCCCCAGTA CCCCCTGGGA AAGCAGGACC CCCAGCCACC 
CAGGCCAAGA GGCAGGAGGA GGACTCGGAG AGCAGCAGCG AGGAGGAGTC GGACAGTGAC 
GGGGACGTGC CAGCTGCTGC ACCTCCAGCC CAGAAAGAGG GTAGCTCCAA AACTGCCACA 
AGCAAGACCC TGGCCCCAGC GTCCCTGGAG AAGAAAGCAG AGGAGTCCTC AACCAGCAGT 
GATGAGGACC TGCCATCCAG CCAGGTGACC AAGAGGAACC TCCCGCTGAC ACAGGCTGCC 
CTGAAGGTTC TTGCCCAGAA AGCCAGTGAG GCCCAATCTC CTGCTGCCAA GACCCCGTCT 
TCAAGTGGGA CTGACAGTGC TGTGGGAACA CTCTCCACGG CAAGTCCCCA GAGCACCCCT 
ACCCAGGTCA GAATGGCCAA CAAGCTCAGA AAGCCTGAGG TTCCTACAGC TCAGCAGGCC 
ACAGCCACTC CCTCGGGACA CCCCACAGCC AAGGCCCCTG CAACCTCAGA TGACAGCAAC 
AGCAGCTCTT CAGGGAGCGA GGACGATGCC AAGGGGTCCC AGGCGGCCCC GTCAGCCCAC 
AGGCCAGGCC CAGCCCCCTC CAGGAGGGAG ACCTTGGTGG AAGAGACCAC AACAGAGTCC 
AGTGACGACG AGGTGGTGGC ACCTTCCCAG TCTCTCCTCT CAGGTTTTGT GACTCCTGGG 
CTGACTCCAG CTGGTTCCAA GACTTCAAAG GCCGCTCCCA AACCAGACGC CAGCCCCTCT 
GTTTCCTCTA CTCCGGCCAC CAGAGATGCC CCAGAGGGCA AGCAGGAAGT GGAGCCCCAA 
CAAGCAGCAG GCACCATGTC CCCTAAAACA GGCAGAAAAG AGGCTGGTGC CACACCTCAG 
AAGCCCGGGA AGGGGCTCGG GAGTCCCCCG GCCTCCATGC TGGCGCTGCA GAGCAACATC 
ACCCAGCGCC TCCTGAGCGA GCCCTGGCCC CTGAGCGAGG CGCAGGTGCA GGCCTCGGTG 
GCGAAGGTGC TCACGGAGCT GCTGGAGCAG GAGAGGAAGA AGGCCATGGA CACCGCCAAG 
GAAGGCAGCC GGAAGGGCCG CCTGGGCCAT AAGCGGAAGC TGTCCGAAGA CCAGACAGCC 
CCCAAGGCCC CCAAGAACAA GAAAAAGAAG CAGCTGGCAG CTGCAGATGG TGGGGAGCAT 
GTCGTCTCCT CAGAAAAGGC CCCCAGGACT GTCAAAGGGA AATCCAAGAA GGACAGAGCA 
AGTGGTGACG TCAAGGAGAA GAAGGAGAAG GAGTCTCCCG GCTCTCAAGG GGCCAAGGAG 
AAGCCAGTAG GGGAGCTGGG GACTCCAAAG GGTGACGGGG GAGACCACGG CAACCTTAAG 
ATGAAGAAAG AGAAGAAGAA ATCTGACAAG AAAAAAAAAG ACAAGGAGAA AAAGGAAAAG 
AAGAAGAAAG CAAAAAAGGC CTCAACCAAA GACCCTGGCT CACCATCACA GAAGAAAAAG 
AAGAGAAAGA AGAAGACAGC AGAGCAAACC GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_010807518.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010805820.1 Bos taurus Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa48671
Clone ID Related Accession (Same CDS sequence)	XM_010807518.2 , XM_010798511.2
Accession Version	XM_010807518.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4185bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	treacle protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104039.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010807518.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAGG CCAGGAAGCG GCGAGAGCTC CTTCCCCTGA TCTACCAGCA TCTGCTGCAG 
GCGGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA AATTTTCCTG 
AGTCAGCCTG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACTTC GGAGATTGGC 
CGGAAACGGA AGGCAGAGGA AGATGCGGCC TTGCAGGCCA AGAGGAGCCG TGTGTCAGAC 
CCCATCAGCA GCTCAGAAAG CTCAGAAGAG GAGGAGGAAG AGGAGGAGGC AGAAGCCAAA 
ACCACCAAAC CCACCCCGAG ATTAGCAGCT ACCAACTCCT CAGTCACAGG GACAGTTTCG 
TCTTCAAATG TGAAAGAAAA AGCCAAGGCA AAGACCACGA AAGCCAGCAA GACGGTGAAC 
TCCACGACAC ACCCTGCCCC GGGAAAGGCA GTGGCCCACC TCCTCACTGG GAAGTCACCC 
CAGAAGACAT CGGGGCCCTC GGCAAATGCT GTCTTGGTGT CAGAAACCGA GGAGGAGGGC 
AGCGTGTCAG CCCTCAGAAC CGCCACCACG CCTGGAATGG CATCCGCCGA CCAGGCTGAC 
AGCTCCAGTG AGGATACCAC CAGCTCGAGT GATGAGACGG ACTTGGAGGC GAAAGCCGCA 
GTAAAACCAC TGCAGGTCAG AGCCTCAGCC ACTGCAGTCA AGGAGTCTCC GAGAAAAAGT 
GCAGCCCCGA CTCCAGGGAA GGCAGGGGTT GTGACACCCC AAGTCAGAGG CGGTGCTGTG 
ACCCCAGCCA GCAGGGCCAA GAAGCCAGAA GAGGTCTCAG AGAGCAGTGA AGAGTCCGAC 
GAGAGTGAGG AGGCCGCCCC TGCGGGGACA CCCAGTCAGG CAAAGCCCTC TGGGAAGATC 
CCCCAGGTTA AAGCTGCCTC CACACCAAAA ATCCAGGTGT TTTTCCCTAA GAAAGGGTCC 
CCTCCAACAT CCCCTGGCAA GGCAGGGCCT GCAGCCACCC AAGCCCAGCC CAGGAAGCAG 
GAGGAGGAGG AGAGCAGCAG CAGCAGCAGC AAGGAATCAG ACAGCGAGGG CGAGGTGCCC 
ACGGCCATGC AGCAGACTAC AAGCCTGGCT CAGGCAAAGC CCTTGGAGAA AAACTCTCAG 
GTCAGAGCTG CCTCAGCCAC AGGTGCAGGA CCCTCGGCAA AGGGCACGGT CCCAGCGCTC 
CCTCAGAAGG CAAGGTCTGT GGCCGCCCAG GATGGGAAGC AGGAGGACTC AGAGAGCAGC 
AGTGAGGAGG AGTCAGACAG CGAGGAGGAG ATGCAGAAGG CAGGGACCTC AGCCCAGGCA 
CAGTCCTCTG GGAAAGCCCT CCAGATCAGA CCTGCCTCCG GTCCCACCAA GAGGCCCCCT 
CAGAAGGCCG GGCCTGCAAC CACCCAGGTC AAGGTTGAAA GGGCCGAGGA AGACTCGGAG 
AGCAGTGAGG AGGAATCAGA CAGTGAGGAT GAAGCTCCTG TAGCCAAGAC TCCAGCCCAG 
GCAAAATCAG CTGTGAAAAC TTCTCAGATC AAGGCCTCCC CAAGGAAGGG CACCCCCATT 
ACACCCTCAT CTGCCAGGGT CCCCCCAGTG CAAGTCGGCA CACCAGCCCC CCGGACAGCA 
GCAGCAGTGA GTACGCCCAC CTGCGCGTCA TCCCCAGCCA TAGTCAGGCA CACCCAGAAG 
CCAGGGGAGG ACTCTTCAAG CAGCGAGGAG TCCGAGAGCG AGGAGGAGAC AGCTCCTGCT 
GCAGCAGGGG GACAGATGAA GTCTATAGGG AAGACCCTCC CAGTGAAAGC TGCCTCACCA 
GCCACCAAGG GGCCCTCAGG AAAAGGGACC ACCCCAGGGC CCCCTGGGAA GGCAGGACCT 
GCAGTGGCAC AGGTCAAGGC TGAGGTGCAG GAAGACTCAG AAAGCAGTGA GGAGTCGGAC 
AATGAGGAAG CGGCTGCAAC TCCCGCACAG GTGAAGACTT CAGTGAAAAC CCCTCAGACC 
AAAGCCAACT CTTCTGCCAC CAGAGTGGCT TCAGCCAAAG CAGCAGCACC AGCTCCTGGA 
AAGGGGGGCC TTGCTGTTGC TCAAGCCAAA GTGGGGTCCC CAGCCAAGGT AAAACCACCA 
GCGAGAACCC CCCAGAGTAG CGCCGTCTCT GGGAGGGGCC AGGTGTCTGT GCCGGCTGTG 
GGGAAGGCAG CAGCTGCAGC AGCCCAGGCC CAGCCGGGGC CTGTCAAGGG CTCACAGGAG 
GACTCGGAGA GCAGTGAGGA GGAGTCGGAC AGTGAGGGAG AGGCACCTCC TCAGGGGAAG 
CCCTCAGGAA AGACCCCCCA GGTCAGAACT GCCTCAGCCC CCACCAAGGC GTCCCCCAAG 
AAAGGGGCTG CCCCAGTACC CCCTGGGAAA GCAGGACCCC CAGCCACCCA GGCCAAGAGG 
CAGGAGGAGG ACTCGGAGAG CAGCAGCGAG GAGGAGTCGG ACAGTGACGG GGACGTGCCA 
GCTGCTGCAC CTCCAGCCCA GAAAGAGGGT AGCTCCAAAA CTGCCACAAG CAAGACCCTG 
GCCCCAGCGT CCCTGGAGAA GAAAGCAGAG GAGTCCTCAA CCAGCAGTGA TGAGGACCTG 
CCATCCAGCC AGGTGATTAA AACCCCTCTG ATTTTTGTCG ACCCTAATCG TAGTCCAGCT 
GGTCCAGCTG CTACCCCCGC CCAAGCGCAG GCTACAGGCA CCCTGAGGAA GGCTCAGGCC 
TTGGAGAGCA CAGCCAGGAG CTCCTCTGAG AGCGAGGACG AGGACGTGAT CCCCGCTACA 
CAGTGCCCGA CTCCTGCCAC TAGGACCAGC GTGGTGACTG TGCCCACGGC ACCCCCAAAG 
GCGGCCCTCA GAGCCAGTGT GGTGGGGGCT CCCAGCAGTG AGGACACAAG TCAGGGGTCT 
GAAGGCAAGA AGCAGGAGGC CTCAGCCACT CAGGTGACCA AGAGGAACCT CCCGCTGACA 
CAGGCTGCCC TGAAGGTTCT TGCCCAGAAA GCCAGTGAGG CCCAATCTCC TGCTGCCAAG 
ACCCCGTCTT CAAGTGGGAC TGACAGTGCT GTGGGAACAC TCTCCACGGC AAGTCCCCAG 
AGCACCCCTA CCCAGGTCAG AATGGCCAAC AAGCTCAGAA AGCCTGAGGT TCCTACAGCT 
CAGCAGGCCA CAGCCACTCC CTCGGGACAC CCCACAGCCA AGGCCCCTGC AACCTCAGAT 
GACAGCAACA GCAGCTCTTC AGGGAGCGAG GACGATGCCA AGGGGTCCCA GGCGGCCCCG 
TCAGCCCACA GGCCAGGCCC AGCCCCCTCC AGGAGGGAGA CCTTGGTGGA AGAGACCACA 
ACAGAGTCCA GTGACGACGA GGTGGTGGCA CCTTCCCAGT CTCTCCTCTC AGGTTTTGTG 
ACTCCTGGGC TGACTCCAGC TGGTTCCAAG ACTTCAAAGG CCGCTCCCAA ACCAGACGCC 
AGCCCCTCTG TTTCCTCTAC TCCGGCCACC AGAGATGCCC CAGAGGGCAA GCAGGAAGTG 
GAGCCCCAAC AAGCAGCAGG CACCATGTCC CCTAAAACAG GCAGAAAAGA GGCTGGTGCC 
ACACCTCAGA AGCCCGGGAA GGGGCTCGGG AGTCCCCCGG CCTCCATGCT GGCGCTGCAG 
AGCAACATCA CCCAGCGCCT CCTGAGCGAG CCCTGGCCCC TGAGCGAGGC GCAGGTGCAG 
GCCTCGGTGG CGAAGGTGCT CACGGAGCTG CTGGAGCAGG AGAGGAAGAA GGCCATGGAC 
ACCGCCAAGG AAGGCAGCCG GAAGGGCCGC CTGGGCCATA AGCGGAAGCT GTCCGAAGAC 
CAGACAGCCC CCAAGGCCCC CAAGAACAAG AAAAAGAAGC AGCTGGCAGC TGCAGATGGT 
GGGGAGCATG TCGTCTCCTC AGAAAAGGCC CCCAGGACTG TCAAAGGGAA ATCCAAGAAG 
GACAGAGCAA GTGGTGACGT CAAGGAGAAG AAGGAGAAGG AGTCTCCCGG CTCTCAAGGG 
GCCAAGGAGA AGCCAGTAGG GGAGCTGGGG ACTCCAAAGG GTGACGGGGG AGACCACGGC 
AACCTTAAGA TGAAGAAAGA GAAGAAGAAA TCTGACAAGA AAAAAAAAGA CAAGGAGAAA 
AAGGAAAAGA AGAAGAAAGC AAAAAAGGCC TCAACCAAAG ACCCTGGCTC ACCATCACAG 
AAGAAAAAGA AGAGAAAGAA GAAGACAGCA GAGCAAACCG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010805820.1
CDS	59..4243
Translation	MAEARKRRELLPLIYQHLLQAGYVRAAREVKEQSGQKIFLSQPVTLLDIYTHWQQTSEIGRKRKAEEDAALQAKRSRVSDPISSSESSEEEEEEEEAEAKTTKPTPRLAATNSSVTGTVSSSNVKEKAKAKTTKASKTVNSTTHPAPGKAVAHLLTGKSPQKTSGPSANAVLVSETEEEGSVSALRTATTPGMASADQADSSSEDTTSSSDETDLEAKAAVKPLQVRASATAVKESPRKSAAPTPGKAGVVTPQVRGGAVTPASRAKKPEEVSESSEESDESEEAAPAGTPSQAKPSGKIPQVKAASTPKIQVFFPKKGSPPTSPGKAGPAATQAQPRKQEEEESSSSSSKESDSEGEVPTAMQQTTSLAQAKPLEKNSQVRAASATGAGPSAKGTVPALPQKARSVAAQDGKQEDSESSSEEESDSEEEMQKAGTSAQAQSSGKALQIRPASGPTKRPPQKAGPATTQVKVERAEEDSESSEEESDSEDEAPVAKTPAQAKSAVKTSQIKASPRKGTPITPSSARVPPVQVGTPAPRTAAAVSTPTCASSPAIVRHTQKPGEDSSSSEESESEEETAPAAAGGQMKSIGKTLPVKAASPATKGPSGKGTTPGPPGKAGPAVAQVKAEVQEDSESSEESDNEEAAATPAQVKTSVKTPQTKANSSATRVASAKAAAPAPGKGGLAVAQAKVGSPAKVKPPARTPQSSAVSGRGQVSVPAVGKAAAAAAQAQPGPVKGSQEDSESSEEESDSEGEAPPQGKPSGKTPQVRTASAPTKASPKKGAAPVPPGKAGPPATQAKRQEEDSESSSEEESDSDGDVPAAAPPAQKEGSSKTATSKTLAPASLEKKAEESSTSSDEDLPSSQVIKTPLIFVDPNRSPAGPAATPAQAQATGTLRKAQALESTARSSSESEDEDVIPATQCPTPATRTSVVTVPTAPPKAALRASVVGAPSSEDTSQGSEGKKQEASATQVTKRNLPLTQAALKVLAQKASEAQSPAAKTPSSSGTDSAVGTLSTASPQSTPTQVRMANKLRKPEVPTAQQATATPSGHPTAKAPATSDDSNSSSSGSEDDAKGSQAAPSAHRPGPAPSRRETLVEETTTESSDDEVVAPSQSLLSGFVTPGLTPAGSKTSKAAPKPDASPSVSSTPATRDAPEGKQEVEPQQAAGTMSPKTGRKEAGATPQKPGKGLGSPPASMLALQSNITQRLLSEPWPLSEAQVQASVAKVLTELLEQERKKAMDTAKEGSRKGRLGHKRKLSEDQTAPKAPKNKKKKQLAAADGGEHVVSSEKAPRTVKGKSKKDRASGDVKEKKEKESPGSQGAKEKPVGELGTPKGDGGDHGNLKMKKEKKKSDKKKKDKEKKEKKKKAKKASTKDPGSPSQKKKKRKKKTAEQTV

Target ORF information:

RefSeq Version	XM_010807518.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010807518.2

ORF Insert Sequence:

ATGGCTGAGG CCAGGAAGCG GCGAGAGCTC CTTCCCCTGA TCTACCAGCA TCTGCTGCAG 
GCGGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA AATTTTCCTG 
AGTCAGCCTG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACTTC GGAGATTGGC 
CGGAAACGGA AGGCAGAGGA AGATGCGGCC TTGCAGGCCA AGAGGAGCCG TGTGTCAGAC 
CCCATCAGCA GCTCAGAAAG CTCAGAAGAG GAGGAGGAAG AGGAGGAGGC AGAAGCCAAA 
ACCACCAAAC CCACCCCGAG ATTAGCAGCT ACCAACTCCT CAGTCACAGG GACAGTTTCG 
TCTTCAAATG TGAAAGAAAA AGCCAAGGCA AAGACCACGA AAGCCAGCAA GACGGTGAAC 
TCCACGACAC ACCCTGCCCC GGGAAAGGCA GTGGCCCACC TCCTCACTGG GAAGTCACCC 
CAGAAGACAT CGGGGCCCTC GGCAAATGCT GTCTTGGTGT CAGAAACCGA GGAGGAGGGC 
AGCGTGTCAG CCCTCAGAAC CGCCACCACG CCTGGAATGG CATCCGCCGA CCAGGCTGAC 
AGCTCCAGTG AGGATACCAC CAGCTCGAGT GATGAGACGG ACTTGGAGGC GAAAGCCGCA 
GTAAAACCAC TGCAGGTCAG AGCCTCAGCC ACTGCAGTCA AGGAGTCTCC GAGAAAAAGT 
GCAGCCCCGA CTCCAGGGAA GGCAGGGGTT GTGACACCCC AAGTCAGAGG CGGTGCTGTG 
ACCCCAGCCA GCAGGGCCAA GAAGCCAGAA GAGGTCTCAG AGAGCAGTGA AGAGTCCGAC 
GAGAGTGAGG AGGCCGCCCC TGCGGGGACA CCCAGTCAGG CAAAGCCCTC TGGGAAGATC 
CCCCAGGTTA AAGCTGCCTC CACACCAAAA ATCCAGGTGT TTTTCCCTAA GAAAGGGTCC 
CCTCCAACAT CCCCTGGCAA GGCAGGGCCT GCAGCCACCC AAGCCCAGCC CAGGAAGCAG 
GAGGAGGAGG AGAGCAGCAG CAGCAGCAGC AAGGAATCAG ACAGCGAGGG CGAGGTGCCC 
ACGGCCATGC AGCAGACTAC AAGCCTGGCT CAGGCAAAGC CCTTGGAGAA AAACTCTCAG 
GTCAGAGCTG CCTCAGCCAC AGGTGCAGGA CCCTCGGCAA AGGGCACGGT CCCAGCGCTC 
CCTCAGAAGG CAAGGTCTGT GGCCGCCCAG GATGGGAAGC AGGAGGACTC AGAGAGCAGC 
AGTGAGGAGG AGTCAGACAG CGAGGAGGAG ATGCAGAAGG CAGGGACCTC AGCCCAGGCA 
CAGTCCTCTG GGAAAGCCCT CCAGATCAGA CCTGCCTCCG GTCCCACCAA GAGGCCCCCT 
CAGAAGGCCG GGCCTGCAAC CACCCAGGTC AAGGTTGAAA GGGCCGAGGA AGACTCGGAG 
AGCAGTGAGG AGGAATCAGA CAGTGAGGAT GAAGCTCCTG TAGCCAAGAC TCCAGCCCAG 
GCAAAATCAG CTGTGAAAAC TTCTCAGATC AAGGCCTCCC CAAGGAAGGG CACCCCCATT 
ACACCCTCAT CTGCCAGGGT CCCCCCAGTG CAAGTCGGCA CACCAGCCCC CCGGACAGCA 
GCAGCAGTGA GTACGCCCAC CTGCGCGTCA TCCCCAGCCA TAGTCAGGCA CACCCAGAAG 
CCAGGGGAGG ACTCTTCAAG CAGCGAGGAG TCCGAGAGCG AGGAGGAGAC AGCTCCTGCT 
GCAGCAGGGG GACAGATGAA GTCTATAGGG AAGACCCTCC CAGTGAAAGC TGCCTCACCA 
GCCACCAAGG GGCCCTCAGG AAAAGGGACC ACCCCAGGGC CCCCTGGGAA GGCAGGACCT 
GCAGTGGCAC AGGTCAAGGC TGAGGTGCAG GAAGACTCAG AAAGCAGTGA GGAGTCGGAC 
AATGAGGAAG CGGCTGCAAC TCCCGCACAG GTGAAGACTT CAGTGAAAAC CCCTCAGACC 
AAAGCCAACT CTTCTGCCAC CAGAGTGGCT TCAGCCAAAG CAGCAGCACC AGCTCCTGGA 
AAGGGGGGCC TTGCTGTTGC TCAAGCCAAA GTGGGGTCCC CAGCCAAGGT AAAACCACCA 
GCGAGAACCC CCCAGAGTAG CGCCGTCTCT GGGAGGGGCC AGGTGTCTGT GCCGGCTGTG 
GGGAAGGCAG CAGCTGCAGC AGCCCAGGCC CAGCCGGGGC CTGTCAAGGG CTCACAGGAG 
GACTCGGAGA GCAGTGAGGA GGAGTCGGAC AGTGAGGGAG AGGCACCTCC TCAGGGGAAG 
CCCTCAGGAA AGACCCCCCA GGTCAGAACT GCCTCAGCCC CCACCAAGGC GTCCCCCAAG 
AAAGGGGCTG CCCCAGTACC CCCTGGGAAA GCAGGACCCC CAGCCACCCA GGCCAAGAGG 
CAGGAGGAGG ACTCGGAGAG CAGCAGCGAG GAGGAGTCGG ACAGTGACGG GGACGTGCCA 
GCTGCTGCAC CTCCAGCCCA GAAAGAGGGT AGCTCCAAAA CTGCCACAAG CAAGACCCTG 
GCCCCAGCGT CCCTGGAGAA GAAAGCAGAG GAGTCCTCAA CCAGCAGTGA TGAGGACCTG 
CCATCCAGCC AGGTGATTAA AACCCCTCTG ATTTTTGTCG ACCCTAATCG TAGTCCAGCT 
GGTCCAGCTG CTACCCCCGC CCAAGCGCAG GCTACAGGCA CCCTGAGGAA GGCTCAGGCC 
TTGGAGAGCA CAGCCAGGAG CTCCTCTGAG AGCGAGGACG AGGACGTGAT CCCCGCTACA 
CAGTGCCCGA CTCCTGCCAC TAGGACCAGC GTGGTGACTG TGCCCACGGC ACCCCCAAAG 
GCGGCCCTCA GAGCCAGTGT GGTGGGGGCT CCCAGCAGTG AGGACACAAG TCAGGGGTCT 
GAAGGCAAGA AGCAGGAGGC CTCAGCCACT CAGGTGACCA AGAGGAACCT CCCGCTGACA 
CAGGCTGCCC TGAAGGTTCT TGCCCAGAAA GCCAGTGAGG CCCAATCTCC TGCTGCCAAG 
ACCCCGTCTT CAAGTGGGAC TGACAGTGCT GTGGGAACAC TCTCCACGGC AAGTCCCCAG 
AGCACCCCTA CCCAGGTCAG AATGGCCAAC AAGCTCAGAA AGCCTGAGGT TCCTACAGCT 
CAGCAGGCCA CAGCCACTCC CTCGGGACAC CCCACAGCCA AGGCCCCTGC AACCTCAGAT 
GACAGCAACA GCAGCTCTTC AGGGAGCGAG GACGATGCCA AGGGGTCCCA GGCGGCCCCG 
TCAGCCCACA GGCCAGGCCC AGCCCCCTCC AGGAGGGAGA CCTTGGTGGA AGAGACCACA 
ACAGAGTCCA GTGACGACGA GGTGGTGGCA CCTTCCCAGT CTCTCCTCTC AGGTTTTGTG 
ACTCCTGGGC TGACTCCAGC TGGTTCCAAG ACTTCAAAGG CCGCTCCCAA ACCAGACGCC 
AGCCCCTCTG TTTCCTCTAC TCCGGCCACC AGAGATGCCC CAGAGGGCAA GCAGGAAGTG 
GAGCCCCAAC AAGCAGCAGG CACCATGTCC CCTAAAACAG GCAGAAAAGA GGCTGGTGCC 
ACACCTCAGA AGCCCGGGAA GGGGCTCGGG AGTCCCCCGG CCTCCATGCT GGCGCTGCAG 
AGCAACATCA CCCAGCGCCT CCTGAGCGAG CCCTGGCCCC TGAGCGAGGC GCAGGTGCAG 
GCCTCGGTGG CGAAGGTGCT CACGGAGCTG CTGGAGCAGG AGAGGAAGAA GGCCATGGAC 
ACCGCCAAGG AAGGCAGCCG GAAGGGCCGC CTGGGCCATA AGCGGAAGCT GTCCGAAGAC 
CAGACAGCCC CCAAGGCCCC CAAGAACAAG AAAAAGAAGC AGCTGGCAGC TGCAGATGGT 
GGGGAGCATG TCGTCTCCTC AGAAAAGGCC CCCAGGACTG TCAAAGGGAA ATCCAAGAAG 
GACAGAGCAA GTGGTGACGT CAAGGAGAAG AAGGAGAAGG AGTCTCCCGG CTCTCAAGGG 
GCCAAGGAGA AGCCAGTAGG GGAGCTGGGG ACTCCAAAGG GTGACGGGGG AGACCACGGC 
AACCTTAAGA TGAAGAAAGA GAAGAAGAAA TCTGACAAGA AAAAAAAAGA CAAGGAGAAA 
AAGGAAAAGA AGAAGAAAGC AAAAAAGGCC TCAACCAAAG ACCCTGGCTC ACCATCACAG 
AAGAAAAAGA AGAGAAAGAA GAAGACAGCA GAGCAAACCG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_010798511.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010796813.1 Bos taurus Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa48671
Clone ID Related Accession (Same CDS sequence)	XM_010807518.2 , XM_010798511.2
Accession Version	XM_010798511.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4185bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	treacle protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645112.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010798511.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAGG CCAGGAAGCG GCGAGAGCTC CTTCCCCTGA TCTACCAGCA TCTGCTGCAG 
GCGGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA AATTTTCCTG 
AGTCAGCCTG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACTTC GGAGATTGGC 
CGGAAACGGA AGGCAGAGGA AGATGCGGCC TTGCAGGCCA AGAGGAGCCG TGTGTCAGAC 
CCCATCAGCA GCTCAGAAAG CTCAGAAGAG GAGGAGGAAG AGGAGGAGGC AGAAGCCAAA 
ACCACCAAAC CCACCCCGAG ATTAGCAGCT ACCAACTCCT CAGTCACAGG GACAGTTTCG 
TCTTCAAATG TGAAAGAAAA AGCCAAGGCA AAGACCACGA AAGCCAGCAA GACGGTGAAC 
TCCACGACAC ACCCTGCCCC GGGAAAGGCA GTGGCCCACC TCCTCACTGG GAAGTCACCC 
CAGAAGACAT CGGGGCCCTC GGCAAATGCT GTCTTGGTGT CAGAAACCGA GGAGGAGGGC 
AGCGTGTCAG CCCTCAGAAC CGCCACCACG CCTGGAATGG CATCCGCCGA CCAGGCTGAC 
AGCTCCAGTG AGGATACCAC CAGCTCGAGT GATGAGACGG ACTTGGAGGC GAAAGCCGCA 
GTAAAACCAC TGCAGGTCAG AGCCTCAGCC ACTGCAGTCA AGGAGTCTCC GAGAAAAAGT 
GCAGCCCCGA CTCCAGGGAA GGCAGGGGTT GTGACACCCC AAGTCAGAGG CGGTGCTGTG 
ACCCCAGCCA GCAGGGCCAA GAAGCCAGAA GAGGTCTCAG AGAGCAGTGA AGAGTCCGAC 
GAGAGTGAGG AGGCCGCCCC TGCGGGGACA CCCAGTCAGG CAAAGCCCTC TGGGAAGATC 
CCCCAGGTTA AAGCTGCCTC CACACCAAAA ATCCAGGTGT TTTTCCCTAA GAAAGGGTCC 
CCTCCAACAT CCCCTGGCAA GGCAGGGCCT GCAGCCACCC AAGCCCAGCC CAGGAAGCAG 
GAGGAGGAGG AGAGCAGCAG CAGCAGCAGC AAGGAATCAG ACAGCGAGGG CGAGGTGCCC 
ACGGCCATGC AGCAGACTAC AAGCCTGGCT CAGGCAAAGC CCTTGGAGAA AAACTCTCAG 
GTCAGAGCTG CCTCAGCCAC AGGTGCAGGA CCCTCGGCAA AGGGCACGGT CCCAGCGCTC 
CCTCAGAAGG CAAGGTCTGT GGCCGCCCAG GATGGGAAGC AGGAGGACTC AGAGAGCAGC 
AGTGAGGAGG AGTCAGACAG CGAGGAGGAG ATGCAGAAGG CAGGGACCTC AGCCCAGGCA 
CAGTCCTCTG GGAAAGCCCT CCAGATCAGA CCTGCCTCCG GTCCCACCAA GAGGCCCCCT 
CAGAAGGCCG GGCCTGCAAC CACCCAGGTC AAGGTTGAAA GGGCCGAGGA AGACTCGGAG 
AGCAGTGAGG AGGAATCAGA CAGTGAGGAT GAAGCTCCTG TAGCCAAGAC TCCAGCCCAG 
GCAAAATCAG CTGTGAAAAC TTCTCAGATC AAGGCCTCCC CAAGGAAGGG CACCCCCATT 
ACACCCTCAT CTGCCAGGGT CCCCCCAGTG CAAGTCGGCA CACCAGCCCC CCGGACAGCA 
GCAGCAGTGA GTACGCCCAC CTGCGCGTCA TCCCCAGCCA TAGTCAGGCA CACCCAGAAG 
CCAGGGGAGG ACTCTTCAAG CAGCGAGGAG TCCGAGAGCG AGGAGGAGAC AGCTCCTGCT 
GCAGCAGGGG GACAGATGAA GTCTATAGGG AAGACCCTCC CAGTGAAAGC TGCCTCACCA 
GCCACCAAGG GGCCCTCAGG AAAAGGGACC ACCCCAGGGC CCCCTGGGAA GGCAGGACCT 
GCAGTGGCAC AGGTCAAGGC TGAGGTGCAG GAAGACTCAG AAAGCAGTGA GGAGTCGGAC 
AATGAGGAAG CGGCTGCAAC TCCCGCACAG GTGAAGACTT CAGTGAAAAC CCCTCAGACC 
AAAGCCAACT CTTCTGCCAC CAGAGTGGCT TCAGCCAAAG CAGCAGCACC AGCTCCTGGA 
AAGGGGGGCC TTGCTGTTGC TCAAGCCAAA GTGGGGTCCC CAGCCAAGGT AAAACCACCA 
GCGAGAACCC CCCAGAGTAG CGCCGTCTCT GGGAGGGGCC AGGTGTCTGT GCCGGCTGTG 
GGGAAGGCAG CAGCTGCAGC AGCCCAGGCC CAGCCGGGGC CTGTCAAGGG CTCACAGGAG 
GACTCGGAGA GCAGTGAGGA GGAGTCGGAC AGTGAGGGAG AGGCACCTCC TCAGGGGAAG 
CCCTCAGGAA AGACCCCCCA GGTCAGAACT GCCTCAGCCC CCACCAAGGC GTCCCCCAAG 
AAAGGGGCTG CCCCAGTACC CCCTGGGAAA GCAGGACCCC CAGCCACCCA GGCCAAGAGG 
CAGGAGGAGG ACTCGGAGAG CAGCAGCGAG GAGGAGTCGG ACAGTGACGG GGACGTGCCA 
GCTGCTGCAC CTCCAGCCCA GAAAGAGGGT AGCTCCAAAA CTGCCACAAG CAAGACCCTG 
GCCCCAGCGT CCCTGGAGAA GAAAGCAGAG GAGTCCTCAA CCAGCAGTGA TGAGGACCTG 
CCATCCAGCC AGGTGATTAA AACCCCTCTG ATTTTTGTCG ACCCTAATCG TAGTCCAGCT 
GGTCCAGCTG CTACCCCCGC CCAAGCGCAG GCTACAGGCA CCCTGAGGAA GGCTCAGGCC 
TTGGAGAGCA CAGCCAGGAG CTCCTCTGAG AGCGAGGACG AGGACGTGAT CCCCGCTACA 
CAGTGCCCGA CTCCTGCCAC TAGGACCAGC GTGGTGACTG TGCCCACGGC ACCCCCAAAG 
GCGGCCCTCA GAGCCAGTGT GGTGGGGGCT CCCAGCAGTG AGGACACAAG TCAGGGGTCT 
GAAGGCAAGA AGCAGGAGGC CTCAGCCACT CAGGTGACCA AGAGGAACCT CCCGCTGACA 
CAGGCTGCCC TGAAGGTTCT TGCCCAGAAA GCCAGTGAGG CCCAATCTCC TGCTGCCAAG 
ACCCCGTCTT CAAGTGGGAC TGACAGTGCT GTGGGAACAC TCTCCACGGC AAGTCCCCAG 
AGCACCCCTA CCCAGGTCAG AATGGCCAAC AAGCTCAGAA AGCCTGAGGT TCCTACAGCT 
CAGCAGGCCA CAGCCACTCC CTCGGGACAC CCCACAGCCA AGGCCCCTGC AACCTCAGAT 
GACAGCAACA GCAGCTCTTC AGGGAGCGAG GACGATGCCA AGGGGTCCCA GGCGGCCCCG 
TCAGCCCACA GGCCAGGCCC AGCCCCCTCC AGGAGGGAGA CCTTGGTGGA AGAGACCACA 
ACAGAGTCCA GTGACGACGA GGTGGTGGCA CCTTCCCAGT CTCTCCTCTC AGGTTTTGTG 
ACTCCTGGGC TGACTCCAGC TGGTTCCAAG ACTTCAAAGG CCGCTCCCAA ACCAGACGCC 
AGCCCCTCTG TTTCCTCTAC TCCGGCCACC AGAGATGCCC CAGAGGGCAA GCAGGAAGTG 
GAGCCCCAAC AAGCAGCAGG CACCATGTCC CCTAAAACAG GCAGAAAAGA GGCTGGTGCC 
ACACCTCAGA AGCCCGGGAA GGGGCTCGGG AGTCCCCCGG CCTCCATGCT GGCGCTGCAG 
AGCAACATCA CCCAGCGCCT CCTGAGCGAG CCCTGGCCCC TGAGCGAGGC GCAGGTGCAG 
GCCTCGGTGG CGAAGGTGCT CACGGAGCTG CTGGAGCAGG AGAGGAAGAA GGCCATGGAC 
ACCGCCAAGG AAGGCAGCCG GAAGGGCCGC CTGGGCCATA AGCGGAAGCT GTCCGAAGAC 
CAGACAGCCC CCAAGGCCCC CAAGAACAAG AAAAAGAAGC AGCTGGCAGC TGCAGATGGT 
GGGGAGCATG TCGTCTCCTC AGAAAAGGCC CCCAGGACTG TCAAAGGGAA ATCCAAGAAG 
GACAGAGCAA GTGGTGACGT CAAGGAGAAG AAGGAGAAGG AGTCTCCCGG CTCTCAAGGG 
GCCAAGGAGA AGCCAGTAGG GGAGCTGGGG ACTCCAAAGG GTGACGGGGG AGACCACGGC 
AACCTTAAGA TGAAGAAAGA GAAGAAGAAA TCTGACAAGA AAAAAAAAGA CAAGGAGAAA 
AAGGAAAAGA AGAAGAAAGC AAAAAAGGCC TCAACCAAAG ACCCTGGCTC ACCATCACAG 
AAGAAAAAGA AGAGAAAGAA GAAGACAGCA GAGCAAACCG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010796813.1
CDS	59..4243
Translation	MAEARKRRELLPLIYQHLLQAGYVRAAREVKEQSGQKIFLSQPVTLLDIYTHWQQTSEIGRKRKAEEDAALQAKRSRVSDPISSSESSEEEEEEEEAEAKTTKPTPRLAATNSSVTGTVSSSNVKEKAKAKTTKASKTVNSTTHPAPGKAVAHLLTGKSPQKTSGPSANAVLVSETEEEGSVSALRTATTPGMASADQADSSSEDTTSSSDETDLEAKAAVKPLQVRASATAVKESPRKSAAPTPGKAGVVTPQVRGGAVTPASRAKKPEEVSESSEESDESEEAAPAGTPSQAKPSGKIPQVKAASTPKIQVFFPKKGSPPTSPGKAGPAATQAQPRKQEEEESSSSSSKESDSEGEVPTAMQQTTSLAQAKPLEKNSQVRAASATGAGPSAKGTVPALPQKARSVAAQDGKQEDSESSSEEESDSEEEMQKAGTSAQAQSSGKALQIRPASGPTKRPPQKAGPATTQVKVERAEEDSESSEEESDSEDEAPVAKTPAQAKSAVKTSQIKASPRKGTPITPSSARVPPVQVGTPAPRTAAAVSTPTCASSPAIVRHTQKPGEDSSSSEESESEEETAPAAAGGQMKSIGKTLPVKAASPATKGPSGKGTTPGPPGKAGPAVAQVKAEVQEDSESSEESDNEEAAATPAQVKTSVKTPQTKANSSATRVASAKAAAPAPGKGGLAVAQAKVGSPAKVKPPARTPQSSAVSGRGQVSVPAVGKAAAAAAQAQPGPVKGSQEDSESSEEESDSEGEAPPQGKPSGKTPQVRTASAPTKASPKKGAAPVPPGKAGPPATQAKRQEEDSESSSEEESDSDGDVPAAAPPAQKEGSSKTATSKTLAPASLEKKAEESSTSSDEDLPSSQVIKTPLIFVDPNRSPAGPAATPAQAQATGTLRKAQALESTARSSSESEDEDVIPATQCPTPATRTSVVTVPTAPPKAALRASVVGAPSSEDTSQGSEGKKQEASATQVTKRNLPLTQAALKVLAQKASEAQSPAAKTPSSSGTDSAVGTLSTASPQSTPTQVRMANKLRKPEVPTAQQATATPSGHPTAKAPATSDDSNSSSSGSEDDAKGSQAAPSAHRPGPAPSRRETLVEETTTESSDDEVVAPSQSLLSGFVTPGLTPAGSKTSKAAPKPDASPSVSSTPATRDAPEGKQEVEPQQAAGTMSPKTGRKEAGATPQKPGKGLGSPPASMLALQSNITQRLLSEPWPLSEAQVQASVAKVLTELLEQERKKAMDTAKEGSRKGRLGHKRKLSEDQTAPKAPKNKKKKQLAAADGGEHVVSSEKAPRTVKGKSKKDRASGDVKEKKEKESPGSQGAKEKPVGELGTPKGDGGDHGNLKMKKEKKKSDKKKKDKEKKEKKKKAKKASTKDPGSPSQKKKKRKKKTAEQTV

Target ORF information:

RefSeq Version	XM_010798511.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010798511.2

ORF Insert Sequence:

ATGGCTGAGG CCAGGAAGCG GCGAGAGCTC CTTCCCCTGA TCTACCAGCA TCTGCTGCAG 
GCGGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA AATTTTCCTG 
AGTCAGCCTG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACTTC GGAGATTGGC 
CGGAAACGGA AGGCAGAGGA AGATGCGGCC TTGCAGGCCA AGAGGAGCCG TGTGTCAGAC 
CCCATCAGCA GCTCAGAAAG CTCAGAAGAG GAGGAGGAAG AGGAGGAGGC AGAAGCCAAA 
ACCACCAAAC CCACCCCGAG ATTAGCAGCT ACCAACTCCT CAGTCACAGG GACAGTTTCG 
TCTTCAAATG TGAAAGAAAA AGCCAAGGCA AAGACCACGA AAGCCAGCAA GACGGTGAAC 
TCCACGACAC ACCCTGCCCC GGGAAAGGCA GTGGCCCACC TCCTCACTGG GAAGTCACCC 
CAGAAGACAT CGGGGCCCTC GGCAAATGCT GTCTTGGTGT CAGAAACCGA GGAGGAGGGC 
AGCGTGTCAG CCCTCAGAAC CGCCACCACG CCTGGAATGG CATCCGCCGA CCAGGCTGAC 
AGCTCCAGTG AGGATACCAC CAGCTCGAGT GATGAGACGG ACTTGGAGGC GAAAGCCGCA 
GTAAAACCAC TGCAGGTCAG AGCCTCAGCC ACTGCAGTCA AGGAGTCTCC GAGAAAAAGT 
GCAGCCCCGA CTCCAGGGAA GGCAGGGGTT GTGACACCCC AAGTCAGAGG CGGTGCTGTG 
ACCCCAGCCA GCAGGGCCAA GAAGCCAGAA GAGGTCTCAG AGAGCAGTGA AGAGTCCGAC 
GAGAGTGAGG AGGCCGCCCC TGCGGGGACA CCCAGTCAGG CAAAGCCCTC TGGGAAGATC 
CCCCAGGTTA AAGCTGCCTC CACACCAAAA ATCCAGGTGT TTTTCCCTAA GAAAGGGTCC 
CCTCCAACAT CCCCTGGCAA GGCAGGGCCT GCAGCCACCC AAGCCCAGCC CAGGAAGCAG 
GAGGAGGAGG AGAGCAGCAG CAGCAGCAGC AAGGAATCAG ACAGCGAGGG CGAGGTGCCC 
ACGGCCATGC AGCAGACTAC AAGCCTGGCT CAGGCAAAGC CCTTGGAGAA AAACTCTCAG 
GTCAGAGCTG CCTCAGCCAC AGGTGCAGGA CCCTCGGCAA AGGGCACGGT CCCAGCGCTC 
CCTCAGAAGG CAAGGTCTGT GGCCGCCCAG GATGGGAAGC AGGAGGACTC AGAGAGCAGC 
AGTGAGGAGG AGTCAGACAG CGAGGAGGAG ATGCAGAAGG CAGGGACCTC AGCCCAGGCA 
CAGTCCTCTG GGAAAGCCCT CCAGATCAGA CCTGCCTCCG GTCCCACCAA GAGGCCCCCT 
CAGAAGGCCG GGCCTGCAAC CACCCAGGTC AAGGTTGAAA GGGCCGAGGA AGACTCGGAG 
AGCAGTGAGG AGGAATCAGA CAGTGAGGAT GAAGCTCCTG TAGCCAAGAC TCCAGCCCAG 
GCAAAATCAG CTGTGAAAAC TTCTCAGATC AAGGCCTCCC CAAGGAAGGG CACCCCCATT 
ACACCCTCAT CTGCCAGGGT CCCCCCAGTG CAAGTCGGCA CACCAGCCCC CCGGACAGCA 
GCAGCAGTGA GTACGCCCAC CTGCGCGTCA TCCCCAGCCA TAGTCAGGCA CACCCAGAAG 
CCAGGGGAGG ACTCTTCAAG CAGCGAGGAG TCCGAGAGCG AGGAGGAGAC AGCTCCTGCT 
GCAGCAGGGG GACAGATGAA GTCTATAGGG AAGACCCTCC CAGTGAAAGC TGCCTCACCA 
GCCACCAAGG GGCCCTCAGG AAAAGGGACC ACCCCAGGGC CCCCTGGGAA GGCAGGACCT 
GCAGTGGCAC AGGTCAAGGC TGAGGTGCAG GAAGACTCAG AAAGCAGTGA GGAGTCGGAC 
AATGAGGAAG CGGCTGCAAC TCCCGCACAG GTGAAGACTT CAGTGAAAAC CCCTCAGACC 
AAAGCCAACT CTTCTGCCAC CAGAGTGGCT TCAGCCAAAG CAGCAGCACC AGCTCCTGGA 
AAGGGGGGCC TTGCTGTTGC TCAAGCCAAA GTGGGGTCCC CAGCCAAGGT AAAACCACCA 
GCGAGAACCC CCCAGAGTAG CGCCGTCTCT GGGAGGGGCC AGGTGTCTGT GCCGGCTGTG 
GGGAAGGCAG CAGCTGCAGC AGCCCAGGCC CAGCCGGGGC CTGTCAAGGG CTCACAGGAG 
GACTCGGAGA GCAGTGAGGA GGAGTCGGAC AGTGAGGGAG AGGCACCTCC TCAGGGGAAG 
CCCTCAGGAA AGACCCCCCA GGTCAGAACT GCCTCAGCCC CCACCAAGGC GTCCCCCAAG 
AAAGGGGCTG CCCCAGTACC CCCTGGGAAA GCAGGACCCC CAGCCACCCA GGCCAAGAGG 
CAGGAGGAGG ACTCGGAGAG CAGCAGCGAG GAGGAGTCGG ACAGTGACGG GGACGTGCCA 
GCTGCTGCAC CTCCAGCCCA GAAAGAGGGT AGCTCCAAAA CTGCCACAAG CAAGACCCTG 
GCCCCAGCGT CCCTGGAGAA GAAAGCAGAG GAGTCCTCAA CCAGCAGTGA TGAGGACCTG 
CCATCCAGCC AGGTGATTAA AACCCCTCTG ATTTTTGTCG ACCCTAATCG TAGTCCAGCT 
GGTCCAGCTG CTACCCCCGC CCAAGCGCAG GCTACAGGCA CCCTGAGGAA GGCTCAGGCC 
TTGGAGAGCA CAGCCAGGAG CTCCTCTGAG AGCGAGGACG AGGACGTGAT CCCCGCTACA 
CAGTGCCCGA CTCCTGCCAC TAGGACCAGC GTGGTGACTG TGCCCACGGC ACCCCCAAAG 
GCGGCCCTCA GAGCCAGTGT GGTGGGGGCT CCCAGCAGTG AGGACACAAG TCAGGGGTCT 
GAAGGCAAGA AGCAGGAGGC CTCAGCCACT CAGGTGACCA AGAGGAACCT CCCGCTGACA 
CAGGCTGCCC TGAAGGTTCT TGCCCAGAAA GCCAGTGAGG CCCAATCTCC TGCTGCCAAG 
ACCCCGTCTT CAAGTGGGAC TGACAGTGCT GTGGGAACAC TCTCCACGGC AAGTCCCCAG 
AGCACCCCTA CCCAGGTCAG AATGGCCAAC AAGCTCAGAA AGCCTGAGGT TCCTACAGCT 
CAGCAGGCCA CAGCCACTCC CTCGGGACAC CCCACAGCCA AGGCCCCTGC AACCTCAGAT 
GACAGCAACA GCAGCTCTTC AGGGAGCGAG GACGATGCCA AGGGGTCCCA GGCGGCCCCG 
TCAGCCCACA GGCCAGGCCC AGCCCCCTCC AGGAGGGAGA CCTTGGTGGA AGAGACCACA 
ACAGAGTCCA GTGACGACGA GGTGGTGGCA CCTTCCCAGT CTCTCCTCTC AGGTTTTGTG 
ACTCCTGGGC TGACTCCAGC TGGTTCCAAG ACTTCAAAGG CCGCTCCCAA ACCAGACGCC 
AGCCCCTCTG TTTCCTCTAC TCCGGCCACC AGAGATGCCC CAGAGGGCAA GCAGGAAGTG 
GAGCCCCAAC AAGCAGCAGG CACCATGTCC CCTAAAACAG GCAGAAAAGA GGCTGGTGCC 
ACACCTCAGA AGCCCGGGAA GGGGCTCGGG AGTCCCCCGG CCTCCATGCT GGCGCTGCAG 
AGCAACATCA CCCAGCGCCT CCTGAGCGAG CCCTGGCCCC TGAGCGAGGC GCAGGTGCAG 
GCCTCGGTGG CGAAGGTGCT CACGGAGCTG CTGGAGCAGG AGAGGAAGAA GGCCATGGAC 
ACCGCCAAGG AAGGCAGCCG GAAGGGCCGC CTGGGCCATA AGCGGAAGCT GTCCGAAGAC 
CAGACAGCCC CCAAGGCCCC CAAGAACAAG AAAAAGAAGC AGCTGGCAGC TGCAGATGGT 
GGGGAGCATG TCGTCTCCTC AGAAAAGGCC CCCAGGACTG TCAAAGGGAA ATCCAAGAAG 
GACAGAGCAA GTGGTGACGT CAAGGAGAAG AAGGAGAAGG AGTCTCCCGG CTCTCAAGGG 
GCCAAGGAGA AGCCAGTAGG GGAGCTGGGG ACTCCAAAGG GTGACGGGGG AGACCACGGC 
AACCTTAAGA TGAAGAAAGA GAAGAAGAAA TCTGACAAGA AAAAAAAAGA CAAGGAGAAA 
AAGGAAAAGA AGAAGAAAGC AAAAAAGGCC TCAACCAAAG ACCCTGGCTC ACCATCACAG 
AAGAAAAAGA AGAGAAAGAA GAAGACAGCA GAGCAAACCG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_010807519.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010805821.1 Bos taurus Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant X7, mRNA.

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CloneID	OBa48672
Clone ID Related Accession (Same CDS sequence)	XM_010807519.2 , XM_010798512.2
Accession Version	XM_010807519.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3939bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	treacle protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104039.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010807519.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAGG CCAGGAAGCG GCGAGAGCTC CTTCCCCTGA TCTACCAGCA TCTGCTGCAG 
GCGGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA AATTTTCCTG 
AGTCAGCCTG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACTTC GGAGATTGGC 
CGGAAACGGA AGGCAGAGGA AGATGCGGCC TTGCAGGCCA AGAGGAGCCG TGTGTCAGAC 
CCCATCAGCA GCTCAGAAAG CTCAGAAGAG GAGGAGGAAG AGGAGGAGGC AGAAGCCAAA 
ACCACCAAAC CCACCCCGAG ATTAGCAGCT ACCAACTCCT CAGTCACAGG GACAGTTTCG 
TCTTCAAATG TGAAAGAAAA AGCCAAGGCA AAGACCACGA AAGCCAGCAA GACGGTGAAC 
TCCACGACAC ACCCTGCCCC GGGAAAGGCA GTGGCCCACC TCCTCACTGG GAAGTCACCC 
CAGAAGACAT CGGGGCCCTC GGCAAATGCT GTCTTGGTGT CAGAAACCGA GGAGGAGGGC 
AGCGTGTCAG CCCTCAGAAC CGCCACCACG CCTGGAATGG CATCCGCCGA CCAGGCTGAC 
AGCTCCAGTG AGGATACCAC CAGCTCGAGT GATGAGACGG ACTTGGAGGC GAAAGCCGCA 
GTAAAACCAC TGCAGGTCAG AGCCTCAGCC ACTGCAGTCA AGGAGTCTCC GAGAAAAAGT 
GCAGCCCCGA CTCCAGGGAA GGCAGGGGTT GTGACACCCC AAGTCAGAGG CGGTGCTGTG 
ACCCCAGCCA GCAGGGCCAA GAAGCCAGAA GAGGTCTCAG AGAGCAGTGA AGAGTCCGAC 
GAGAGTGAGG AGGCCGCCCC TGCGGGGACA CCCAGTCAGG TGAAGGCCTT GGAGAAAACC 
GTCCCAATCA GAGTTGCTCC TAGTCCTGCC AAGGGGACCC CTGGGAAAGG GGCCACCCCA 
GCACCCCCTG GGAAGGCAGG GCCCTTAGGG AAGCCAGAGG AGGACTCGGA GAGCAGCAGC 
GAGGAGTCAG ACAGCGAGGA GGAGATGCAG AAGGCAGGGA CCTCAGCCCA GGCACAGTCC 
TCTGGGAAAG CCCTCCAGAT CAGACCTGCC TCCGGTCCCA CCAAGAGGCC CCCTCAGAAG 
GCCGGGCCTG CAACCACCCA GGTCAAGGTT GAAAGGGCCG AGGAAGACTC GGAGAGCAGT 
GAGGAGGAAT CAGACAGTGA GGATGAAGCT CCTGTAGCCA AGACTCCAGC CCAGGCAAAA 
TCAGCTGTGA AAACTTCTCA GATCAAGGCC TCCCCAAGGA AGGGCACCCC CATTACACCC 
TCATCTGCCA GGGTCCCCCC AGTGCAAGTC GGCACACCAG CCCCCCGGAC AGCAGCAGCA 
GTGAGTACGC CCACCTGCGC GTCATCCCCA GCCATAGTCA GGCACACCCA GAAGCCAGGG 
GAGGACTCTT CAAGCAGCGA GGAGTCCGAG AGCGAGGAGG AGACAGCTCC TGCTGCAGCA 
GGGGGACAGA TGAAGTCTAT AGGGAAGACC CTCCCAGTGA AAGCTGCCTC ACCAGCCACC 
AAGGGGCCCT CAGGAAAAGG GACCACCCCA GGGCCCCCTG GGAAGGCAGG ACCTGCAGTG 
GCACAGGTCA AGGCTGAGGT GCAGGAAGAC TCAGAAAGCA GTGAGGAGTC GGACAATGAG 
GAAGCGGCTG CAACTCCCGC ACAGGTGAAG ACTTCAGTGA AAACCCCTCA GACCAAAGCC 
AACTCTTCTG CCACCAGAGT GGCTTCAGCC AAAGCAGCAG CACCAGCTCC TGGAAAGGGG 
GGCCTTGCTG TTGCTCAAGC CAAAGTGGGG TCCCCAGCCA AGGTAAAACC ACCAGCGAGA 
ACCCCCCAGA GTAGCGCCGT CTCTGGGAGG GGCCAGGTGT CTGTGCCGGC TGTGGGGAAG 
GCAGCAGCTG CAGCAGCCCA GGCCCAGCCG GGGCCTGTCA AGGGCTCACA GGAGGACTCG 
GAGAGCAGTG AGGAGGAGTC GGACAGTGAG GGAGAGGCAC CTCCTCAGGG GAAGCCCTCA 
GGAAAGACCC CCCAGGTCAG AACTGCCTCA GCCCCCACCA AGGCGTCCCC CAAGAAAGGG 
GCTGCCCCAG TACCCCCTGG GAAAGCAGGA CCCCCAGCCA CCCAGGCCAA GAGGCAGGAG 
GAGGACTCGG AGAGCAGCAG CGAGGAGGAG TCGGACAGTG ACGGGGACGT GCCAGCTGCT 
GCACCTCCAG CCCAGAAAGA GGGTAGCTCC AAAACTGCCA CAAGCAAGAC CCTGGCCCCA 
GCGTCCCTGG AGAAGAAAGC AGAGGAGTCC TCAACCAGCA GTGATGAGGA CCTGCCATCC 
AGCCAGGTGA TTAAAACCCC TCTGATTTTT GTCGACCCTA ATCGTAGTCC AGCTGGTCCA 
GCTGCTACCC CCGCCCAAGC GCAGGCTACA GGCACCCTGA GGAAGGCTCA GGCCTTGGAG 
AGCACAGCCA GGAGCTCCTC TGAGAGCGAG GACGAGGACG TGATCCCCGC TACACAGTGC 
CCGACTCCTG CCACTAGGAC CAGCGTGGTG ACTGTGCCCA CGGCACCCCC AAAGGCGGCC 
CTCAGAGCCA GTGTGGTGGG GGCTCCCAGC AGTGAGGACA CAAGTCAGGG GTCTGAAGGC 
AAGAAGCAGG AGGCCTCAGC CACTCAGGTG ACCAAGAGGA ACCTCCCGCT GACACAGGCT 
GCCCTGAAGG TTCTTGCCCA GAAAGCCAGT GAGGCCCAAT CTCCTGCTGC CAAGACCCCG 
TCTTCAAGTG GGACTGACAG TGCTGTGGGA ACACTCTCCA CGGCAAGTCC CCAGAGCACC 
CCTACCCAGG TCAGAATGGC CAACAAGCTC AGAAAGCCTG AGGTTCCTAC AGCTCAGCAG 
GCCACAGCCA CTCCCTCGGG ACACCCCACA GCCAAGGCCC CTGCAACCTC AGATGACAGC 
AACAGCAGCT CTTCAGGGAG CGAGGACGAT GCCAAGGGGT CCCAGGCGGC CCCGTCAGCC 
CACAGGCCAG GCCCAGCCCC CTCCAGGAGG GAGACCTTGG TGGAAGAGAC CACAACAGAG 
TCCAGTGACG ACGAGGTGGT GGCACCTTCC CAGTCTCTCC TCTCAGGTTT TGTGACTCCT 
GGGCTGACTC CAGCTGGTTC CAAGACTTCA AAGGCCGCTC CCAAACCAGA CGCCAGCCCC 
TCTGTTTCCT CTACTCCGGC CACCAGAGAT GCCCCAGAGG GCAAGCAGGA AGTGGAGCCC 
CAACAAGCAG CAGGCACCAT GTCCCCTAAA ACAGGCAGAA AAGAGGCTGG TGCCACACCT 
CAGAAGCCCG GGAAGGGGCT CGGGAGTCCC CCGGCCTCCA TGCTGGCGCT GCAGAGCAAC 
ATCACCCAGC GCCTCCTGAG CGAGCCCTGG CCCCTGAGCG AGGCGCAGGT GCAGGCCTCG 
GTGGCGAAGG TGCTCACGGA GCTGCTGGAG CAGGAGAGGA AGAAGGCCAT GGACACCGCC 
AAGGAAGGCA GCCGGAAGGG CCGCCTGGGC CATAAGCGGA AGCTGTCCGA AGACCAGACA 
GCCCCCAAGG CCCCCAAGAA CAAGAAAAAG AAGCAGCTGG CAGCTGCAGA TGGTGGGGAG 
CATGTCGTCT CCTCAGAAAA GGCCCCCAGG ACTGTCAAAG GGAAATCCAA GAAGGACAGA 
GCAAGTGGTG ACGTCAAGGA GAAGAAGGAG AAGGAGTCTC CCGGCTCTCA AGGGGCCAAG 
GAGAAGCCAG TAGGGGAGCT GGGGACTCCA AAGGGTGACG GGGGAGACCA CGGCAACCTT 
AAGATGAAGA AAGAGAAGAA GAAATCTGAC AAGAAAAAAA AAGACAAGGA GAAAAAGGAA 
AAGAAGAAGA AAGCAAAAAA GGCCTCAACC AAAGACCCTG GCTCACCATC ACAGAAGAAA 
AAGAAGAGAA AGAAGAAGAC AGCAGAGCAA ACCGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010805821.1
CDS	59..3997
Translation	MAEARKRRELLPLIYQHLLQAGYVRAAREVKEQSGQKIFLSQPVTLLDIYTHWQQTSEIGRKRKAEEDAALQAKRSRVSDPISSSESSEEEEEEEEAEAKTTKPTPRLAATNSSVTGTVSSSNVKEKAKAKTTKASKTVNSTTHPAPGKAVAHLLTGKSPQKTSGPSANAVLVSETEEEGSVSALRTATTPGMASADQADSSSEDTTSSSDETDLEAKAAVKPLQVRASATAVKESPRKSAAPTPGKAGVVTPQVRGGAVTPASRAKKPEEVSESSEESDESEEAAPAGTPSQVKALEKTVPIRVAPSPAKGTPGKGATPAPPGKAGPLGKPEEDSESSSEESDSEEEMQKAGTSAQAQSSGKALQIRPASGPTKRPPQKAGPATTQVKVERAEEDSESSEEESDSEDEAPVAKTPAQAKSAVKTSQIKASPRKGTPITPSSARVPPVQVGTPAPRTAAAVSTPTCASSPAIVRHTQKPGEDSSSSEESESEEETAPAAAGGQMKSIGKTLPVKAASPATKGPSGKGTTPGPPGKAGPAVAQVKAEVQEDSESSEESDNEEAAATPAQVKTSVKTPQTKANSSATRVASAKAAAPAPGKGGLAVAQAKVGSPAKVKPPARTPQSSAVSGRGQVSVPAVGKAAAAAAQAQPGPVKGSQEDSESSEEESDSEGEAPPQGKPSGKTPQVRTASAPTKASPKKGAAPVPPGKAGPPATQAKRQEEDSESSSEEESDSDGDVPAAAPPAQKEGSSKTATSKTLAPASLEKKAEESSTSSDEDLPSSQVIKTPLIFVDPNRSPAGPAATPAQAQATGTLRKAQALESTARSSSESEDEDVIPATQCPTPATRTSVVTVPTAPPKAALRASVVGAPSSEDTSQGSEGKKQEASATQVTKRNLPLTQAALKVLAQKASEAQSPAAKTPSSSGTDSAVGTLSTASPQSTPTQVRMANKLRKPEVPTAQQATATPSGHPTAKAPATSDDSNSSSSGSEDDAKGSQAAPSAHRPGPAPSRRETLVEETTTESSDDEVVAPSQSLLSGFVTPGLTPAGSKTSKAAPKPDASPSVSSTPATRDAPEGKQEVEPQQAAGTMSPKTGRKEAGATPQKPGKGLGSPPASMLALQSNITQRLLSEPWPLSEAQVQASVAKVLTELLEQERKKAMDTAKEGSRKGRLGHKRKLSEDQTAPKAPKNKKKKQLAAADGGEHVVSSEKAPRTVKGKSKKDRASGDVKEKKEKESPGSQGAKEKPVGELGTPKGDGGDHGNLKMKKEKKKSDKKKKDKEKKEKKKKAKKASTKDPGSPSQKKKKRKKKTAEQTV

Target ORF information:

RefSeq Version	XM_010807519.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010807519.2

ORF Insert Sequence:

ATGGCTGAGG CCAGGAAGCG GCGAGAGCTC CTTCCCCTGA TCTACCAGCA TCTGCTGCAG 
GCGGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA AATTTTCCTG 
AGTCAGCCTG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACTTC GGAGATTGGC 
CGGAAACGGA AGGCAGAGGA AGATGCGGCC TTGCAGGCCA AGAGGAGCCG TGTGTCAGAC 
CCCATCAGCA GCTCAGAAAG CTCAGAAGAG GAGGAGGAAG AGGAGGAGGC AGAAGCCAAA 
ACCACCAAAC CCACCCCGAG ATTAGCAGCT ACCAACTCCT CAGTCACAGG GACAGTTTCG 
TCTTCAAATG TGAAAGAAAA AGCCAAGGCA AAGACCACGA AAGCCAGCAA GACGGTGAAC 
TCCACGACAC ACCCTGCCCC GGGAAAGGCA GTGGCCCACC TCCTCACTGG GAAGTCACCC 
CAGAAGACAT CGGGGCCCTC GGCAAATGCT GTCTTGGTGT CAGAAACCGA GGAGGAGGGC 
AGCGTGTCAG CCCTCAGAAC CGCCACCACG CCTGGAATGG CATCCGCCGA CCAGGCTGAC 
AGCTCCAGTG AGGATACCAC CAGCTCGAGT GATGAGACGG ACTTGGAGGC GAAAGCCGCA 
GTAAAACCAC TGCAGGTCAG AGCCTCAGCC ACTGCAGTCA AGGAGTCTCC GAGAAAAAGT 
GCAGCCCCGA CTCCAGGGAA GGCAGGGGTT GTGACACCCC AAGTCAGAGG CGGTGCTGTG 
ACCCCAGCCA GCAGGGCCAA GAAGCCAGAA GAGGTCTCAG AGAGCAGTGA AGAGTCCGAC 
GAGAGTGAGG AGGCCGCCCC TGCGGGGACA CCCAGTCAGG TGAAGGCCTT GGAGAAAACC 
GTCCCAATCA GAGTTGCTCC TAGTCCTGCC AAGGGGACCC CTGGGAAAGG GGCCACCCCA 
GCACCCCCTG GGAAGGCAGG GCCCTTAGGG AAGCCAGAGG AGGACTCGGA GAGCAGCAGC 
GAGGAGTCAG ACAGCGAGGA GGAGATGCAG AAGGCAGGGA CCTCAGCCCA GGCACAGTCC 
TCTGGGAAAG CCCTCCAGAT CAGACCTGCC TCCGGTCCCA CCAAGAGGCC CCCTCAGAAG 
GCCGGGCCTG CAACCACCCA GGTCAAGGTT GAAAGGGCCG AGGAAGACTC GGAGAGCAGT 
GAGGAGGAAT CAGACAGTGA GGATGAAGCT CCTGTAGCCA AGACTCCAGC CCAGGCAAAA 
TCAGCTGTGA AAACTTCTCA GATCAAGGCC TCCCCAAGGA AGGGCACCCC CATTACACCC 
TCATCTGCCA GGGTCCCCCC AGTGCAAGTC GGCACACCAG CCCCCCGGAC AGCAGCAGCA 
GTGAGTACGC CCACCTGCGC GTCATCCCCA GCCATAGTCA GGCACACCCA GAAGCCAGGG 
GAGGACTCTT CAAGCAGCGA GGAGTCCGAG AGCGAGGAGG AGACAGCTCC TGCTGCAGCA 
GGGGGACAGA TGAAGTCTAT AGGGAAGACC CTCCCAGTGA AAGCTGCCTC ACCAGCCACC 
AAGGGGCCCT CAGGAAAAGG GACCACCCCA GGGCCCCCTG GGAAGGCAGG ACCTGCAGTG 
GCACAGGTCA AGGCTGAGGT GCAGGAAGAC TCAGAAAGCA GTGAGGAGTC GGACAATGAG 
GAAGCGGCTG CAACTCCCGC ACAGGTGAAG ACTTCAGTGA AAACCCCTCA GACCAAAGCC 
AACTCTTCTG CCACCAGAGT GGCTTCAGCC AAAGCAGCAG CACCAGCTCC TGGAAAGGGG 
GGCCTTGCTG TTGCTCAAGC CAAAGTGGGG TCCCCAGCCA AGGTAAAACC ACCAGCGAGA 
ACCCCCCAGA GTAGCGCCGT CTCTGGGAGG GGCCAGGTGT CTGTGCCGGC TGTGGGGAAG 
GCAGCAGCTG CAGCAGCCCA GGCCCAGCCG GGGCCTGTCA AGGGCTCACA GGAGGACTCG 
GAGAGCAGTG AGGAGGAGTC GGACAGTGAG GGAGAGGCAC CTCCTCAGGG GAAGCCCTCA 
GGAAAGACCC CCCAGGTCAG AACTGCCTCA GCCCCCACCA AGGCGTCCCC CAAGAAAGGG 
GCTGCCCCAG TACCCCCTGG GAAAGCAGGA CCCCCAGCCA CCCAGGCCAA GAGGCAGGAG 
GAGGACTCGG AGAGCAGCAG CGAGGAGGAG TCGGACAGTG ACGGGGACGT GCCAGCTGCT 
GCACCTCCAG CCCAGAAAGA GGGTAGCTCC AAAACTGCCA CAAGCAAGAC CCTGGCCCCA 
GCGTCCCTGG AGAAGAAAGC AGAGGAGTCC TCAACCAGCA GTGATGAGGA CCTGCCATCC 
AGCCAGGTGA TTAAAACCCC TCTGATTTTT GTCGACCCTA ATCGTAGTCC AGCTGGTCCA 
GCTGCTACCC CCGCCCAAGC GCAGGCTACA GGCACCCTGA GGAAGGCTCA GGCCTTGGAG 
AGCACAGCCA GGAGCTCCTC TGAGAGCGAG GACGAGGACG TGATCCCCGC TACACAGTGC 
CCGACTCCTG CCACTAGGAC CAGCGTGGTG ACTGTGCCCA CGGCACCCCC AAAGGCGGCC 
CTCAGAGCCA GTGTGGTGGG GGCTCCCAGC AGTGAGGACA CAAGTCAGGG GTCTGAAGGC 
AAGAAGCAGG AGGCCTCAGC CACTCAGGTG ACCAAGAGGA ACCTCCCGCT GACACAGGCT 
GCCCTGAAGG TTCTTGCCCA GAAAGCCAGT GAGGCCCAAT CTCCTGCTGC CAAGACCCCG 
TCTTCAAGTG GGACTGACAG TGCTGTGGGA ACACTCTCCA CGGCAAGTCC CCAGAGCACC 
CCTACCCAGG TCAGAATGGC CAACAAGCTC AGAAAGCCTG AGGTTCCTAC AGCTCAGCAG 
GCCACAGCCA CTCCCTCGGG ACACCCCACA GCCAAGGCCC CTGCAACCTC AGATGACAGC 
AACAGCAGCT CTTCAGGGAG CGAGGACGAT GCCAAGGGGT CCCAGGCGGC CCCGTCAGCC 
CACAGGCCAG GCCCAGCCCC CTCCAGGAGG GAGACCTTGG TGGAAGAGAC CACAACAGAG 
TCCAGTGACG ACGAGGTGGT GGCACCTTCC CAGTCTCTCC TCTCAGGTTT TGTGACTCCT 
GGGCTGACTC CAGCTGGTTC CAAGACTTCA AAGGCCGCTC CCAAACCAGA CGCCAGCCCC 
TCTGTTTCCT CTACTCCGGC CACCAGAGAT GCCCCAGAGG GCAAGCAGGA AGTGGAGCCC 
CAACAAGCAG CAGGCACCAT GTCCCCTAAA ACAGGCAGAA AAGAGGCTGG TGCCACACCT 
CAGAAGCCCG GGAAGGGGCT CGGGAGTCCC CCGGCCTCCA TGCTGGCGCT GCAGAGCAAC 
ATCACCCAGC GCCTCCTGAG CGAGCCCTGG CCCCTGAGCG AGGCGCAGGT GCAGGCCTCG 
GTGGCGAAGG TGCTCACGGA GCTGCTGGAG CAGGAGAGGA AGAAGGCCAT GGACACCGCC 
AAGGAAGGCA GCCGGAAGGG CCGCCTGGGC CATAAGCGGA AGCTGTCCGA AGACCAGACA 
GCCCCCAAGG CCCCCAAGAA CAAGAAAAAG AAGCAGCTGG CAGCTGCAGA TGGTGGGGAG 
CATGTCGTCT CCTCAGAAAA GGCCCCCAGG ACTGTCAAAG GGAAATCCAA GAAGGACAGA 
GCAAGTGGTG ACGTCAAGGA GAAGAAGGAG AAGGAGTCTC CCGGCTCTCA AGGGGCCAAG 
GAGAAGCCAG TAGGGGAGCT GGGGACTCCA AAGGGTGACG GGGGAGACCA CGGCAACCTT 
AAGATGAAGA AAGAGAAGAA GAAATCTGAC AAGAAAAAAA AAGACAAGGA GAAAAAGGAA 
AAGAAGAAGA AAGCAAAAAA GGCCTCAACC AAAGACCCTG GCTCACCATC ACAGAAGAAA 
AAGAAGAGAA AGAAGAAGAC AGCAGAGCAA ACCGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_010798512.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010796814.1 Bos taurus Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant X7, mRNA.

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CloneID	OBa48672
Clone ID Related Accession (Same CDS sequence)	XM_010807519.2 , XM_010798512.2
Accession Version	XM_010798512.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3939bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	treacle protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645112.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010798512.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAGG CCAGGAAGCG GCGAGAGCTC CTTCCCCTGA TCTACCAGCA TCTGCTGCAG 
GCGGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA AATTTTCCTG 
AGTCAGCCTG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACTTC GGAGATTGGC 
CGGAAACGGA AGGCAGAGGA AGATGCGGCC TTGCAGGCCA AGAGGAGCCG TGTGTCAGAC 
CCCATCAGCA GCTCAGAAAG CTCAGAAGAG GAGGAGGAAG AGGAGGAGGC AGAAGCCAAA 
ACCACCAAAC CCACCCCGAG ATTAGCAGCT ACCAACTCCT CAGTCACAGG GACAGTTTCG 
TCTTCAAATG TGAAAGAAAA AGCCAAGGCA AAGACCACGA AAGCCAGCAA GACGGTGAAC 
TCCACGACAC ACCCTGCCCC GGGAAAGGCA GTGGCCCACC TCCTCACTGG GAAGTCACCC 
CAGAAGACAT CGGGGCCCTC GGCAAATGCT GTCTTGGTGT CAGAAACCGA GGAGGAGGGC 
AGCGTGTCAG CCCTCAGAAC CGCCACCACG CCTGGAATGG CATCCGCCGA CCAGGCTGAC 
AGCTCCAGTG AGGATACCAC CAGCTCGAGT GATGAGACGG ACTTGGAGGC GAAAGCCGCA 
GTAAAACCAC TGCAGGTCAG AGCCTCAGCC ACTGCAGTCA AGGAGTCTCC GAGAAAAAGT 
GCAGCCCCGA CTCCAGGGAA GGCAGGGGTT GTGACACCCC AAGTCAGAGG CGGTGCTGTG 
ACCCCAGCCA GCAGGGCCAA GAAGCCAGAA GAGGTCTCAG AGAGCAGTGA AGAGTCCGAC 
GAGAGTGAGG AGGCCGCCCC TGCGGGGACA CCCAGTCAGG TGAAGGCCTT GGAGAAAACC 
GTCCCAATCA GAGTTGCTCC TAGTCCTGCC AAGGGGACCC CTGGGAAAGG GGCCACCCCA 
GCACCCCCTG GGAAGGCAGG GCCCTTAGGG AAGCCAGAGG AGGACTCGGA GAGCAGCAGC 
GAGGAGTCAG ACAGCGAGGA GGAGATGCAG AAGGCAGGGA CCTCAGCCCA GGCACAGTCC 
TCTGGGAAAG CCCTCCAGAT CAGACCTGCC TCCGGTCCCA CCAAGAGGCC CCCTCAGAAG 
GCCGGGCCTG CAACCACCCA GGTCAAGGTT GAAAGGGCCG AGGAAGACTC GGAGAGCAGT 
GAGGAGGAAT CAGACAGTGA GGATGAAGCT CCTGTAGCCA AGACTCCAGC CCAGGCAAAA 
TCAGCTGTGA AAACTTCTCA GATCAAGGCC TCCCCAAGGA AGGGCACCCC CATTACACCC 
TCATCTGCCA GGGTCCCCCC AGTGCAAGTC GGCACACCAG CCCCCCGGAC AGCAGCAGCA 
GTGAGTACGC CCACCTGCGC GTCATCCCCA GCCATAGTCA GGCACACCCA GAAGCCAGGG 
GAGGACTCTT CAAGCAGCGA GGAGTCCGAG AGCGAGGAGG AGACAGCTCC TGCTGCAGCA 
GGGGGACAGA TGAAGTCTAT AGGGAAGACC CTCCCAGTGA AAGCTGCCTC ACCAGCCACC 
AAGGGGCCCT CAGGAAAAGG GACCACCCCA GGGCCCCCTG GGAAGGCAGG ACCTGCAGTG 
GCACAGGTCA AGGCTGAGGT GCAGGAAGAC TCAGAAAGCA GTGAGGAGTC GGACAATGAG 
GAAGCGGCTG CAACTCCCGC ACAGGTGAAG ACTTCAGTGA AAACCCCTCA GACCAAAGCC 
AACTCTTCTG CCACCAGAGT GGCTTCAGCC AAAGCAGCAG CACCAGCTCC TGGAAAGGGG 
GGCCTTGCTG TTGCTCAAGC CAAAGTGGGG TCCCCAGCCA AGGTAAAACC ACCAGCGAGA 
ACCCCCCAGA GTAGCGCCGT CTCTGGGAGG GGCCAGGTGT CTGTGCCGGC TGTGGGGAAG 
GCAGCAGCTG CAGCAGCCCA GGCCCAGCCG GGGCCTGTCA AGGGCTCACA GGAGGACTCG 
GAGAGCAGTG AGGAGGAGTC GGACAGTGAG GGAGAGGCAC CTCCTCAGGG GAAGCCCTCA 
GGAAAGACCC CCCAGGTCAG AACTGCCTCA GCCCCCACCA AGGCGTCCCC CAAGAAAGGG 
GCTGCCCCAG TACCCCCTGG GAAAGCAGGA CCCCCAGCCA CCCAGGCCAA GAGGCAGGAG 
GAGGACTCGG AGAGCAGCAG CGAGGAGGAG TCGGACAGTG ACGGGGACGT GCCAGCTGCT 
GCACCTCCAG CCCAGAAAGA GGGTAGCTCC AAAACTGCCA CAAGCAAGAC CCTGGCCCCA 
GCGTCCCTGG AGAAGAAAGC AGAGGAGTCC TCAACCAGCA GTGATGAGGA CCTGCCATCC 
AGCCAGGTGA TTAAAACCCC TCTGATTTTT GTCGACCCTA ATCGTAGTCC AGCTGGTCCA 
GCTGCTACCC CCGCCCAAGC GCAGGCTACA GGCACCCTGA GGAAGGCTCA GGCCTTGGAG 
AGCACAGCCA GGAGCTCCTC TGAGAGCGAG GACGAGGACG TGATCCCCGC TACACAGTGC 
CCGACTCCTG CCACTAGGAC CAGCGTGGTG ACTGTGCCCA CGGCACCCCC AAAGGCGGCC 
CTCAGAGCCA GTGTGGTGGG GGCTCCCAGC AGTGAGGACA CAAGTCAGGG GTCTGAAGGC 
AAGAAGCAGG AGGCCTCAGC CACTCAGGTG ACCAAGAGGA ACCTCCCGCT GACACAGGCT 
GCCCTGAAGG TTCTTGCCCA GAAAGCCAGT GAGGCCCAAT CTCCTGCTGC CAAGACCCCG 
TCTTCAAGTG GGACTGACAG TGCTGTGGGA ACACTCTCCA CGGCAAGTCC CCAGAGCACC 
CCTACCCAGG TCAGAATGGC CAACAAGCTC AGAAAGCCTG AGGTTCCTAC AGCTCAGCAG 
GCCACAGCCA CTCCCTCGGG ACACCCCACA GCCAAGGCCC CTGCAACCTC AGATGACAGC 
AACAGCAGCT CTTCAGGGAG CGAGGACGAT GCCAAGGGGT CCCAGGCGGC CCCGTCAGCC 
CACAGGCCAG GCCCAGCCCC CTCCAGGAGG GAGACCTTGG TGGAAGAGAC CACAACAGAG 
TCCAGTGACG ACGAGGTGGT GGCACCTTCC CAGTCTCTCC TCTCAGGTTT TGTGACTCCT 
GGGCTGACTC CAGCTGGTTC CAAGACTTCA AAGGCCGCTC CCAAACCAGA CGCCAGCCCC 
TCTGTTTCCT CTACTCCGGC CACCAGAGAT GCCCCAGAGG GCAAGCAGGA AGTGGAGCCC 
CAACAAGCAG CAGGCACCAT GTCCCCTAAA ACAGGCAGAA AAGAGGCTGG TGCCACACCT 
CAGAAGCCCG GGAAGGGGCT CGGGAGTCCC CCGGCCTCCA TGCTGGCGCT GCAGAGCAAC 
ATCACCCAGC GCCTCCTGAG CGAGCCCTGG CCCCTGAGCG AGGCGCAGGT GCAGGCCTCG 
GTGGCGAAGG TGCTCACGGA GCTGCTGGAG CAGGAGAGGA AGAAGGCCAT GGACACCGCC 
AAGGAAGGCA GCCGGAAGGG CCGCCTGGGC CATAAGCGGA AGCTGTCCGA AGACCAGACA 
GCCCCCAAGG CCCCCAAGAA CAAGAAAAAG AAGCAGCTGG CAGCTGCAGA TGGTGGGGAG 
CATGTCGTCT CCTCAGAAAA GGCCCCCAGG ACTGTCAAAG GGAAATCCAA GAAGGACAGA 
GCAAGTGGTG ACGTCAAGGA GAAGAAGGAG AAGGAGTCTC CCGGCTCTCA AGGGGCCAAG 
GAGAAGCCAG TAGGGGAGCT GGGGACTCCA AAGGGTGACG GGGGAGACCA CGGCAACCTT 
AAGATGAAGA AAGAGAAGAA GAAATCTGAC AAGAAAAAAA AAGACAAGGA GAAAAAGGAA 
AAGAAGAAGA AAGCAAAAAA GGCCTCAACC AAAGACCCTG GCTCACCATC ACAGAAGAAA 
AAGAAGAGAA AGAAGAAGAC AGCAGAGCAA ACCGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010796814.1
CDS	59..3997
Translation	MAEARKRRELLPLIYQHLLQAGYVRAAREVKEQSGQKIFLSQPVTLLDIYTHWQQTSEIGRKRKAEEDAALQAKRSRVSDPISSSESSEEEEEEEEAEAKTTKPTPRLAATNSSVTGTVSSSNVKEKAKAKTTKASKTVNSTTHPAPGKAVAHLLTGKSPQKTSGPSANAVLVSETEEEGSVSALRTATTPGMASADQADSSSEDTTSSSDETDLEAKAAVKPLQVRASATAVKESPRKSAAPTPGKAGVVTPQVRGGAVTPASRAKKPEEVSESSEESDESEEAAPAGTPSQVKALEKTVPIRVAPSPAKGTPGKGATPAPPGKAGPLGKPEEDSESSSEESDSEEEMQKAGTSAQAQSSGKALQIRPASGPTKRPPQKAGPATTQVKVERAEEDSESSEEESDSEDEAPVAKTPAQAKSAVKTSQIKASPRKGTPITPSSARVPPVQVGTPAPRTAAAVSTPTCASSPAIVRHTQKPGEDSSSSEESESEEETAPAAAGGQMKSIGKTLPVKAASPATKGPSGKGTTPGPPGKAGPAVAQVKAEVQEDSESSEESDNEEAAATPAQVKTSVKTPQTKANSSATRVASAKAAAPAPGKGGLAVAQAKVGSPAKVKPPARTPQSSAVSGRGQVSVPAVGKAAAAAAQAQPGPVKGSQEDSESSEEESDSEGEAPPQGKPSGKTPQVRTASAPTKASPKKGAAPVPPGKAGPPATQAKRQEEDSESSSEEESDSDGDVPAAAPPAQKEGSSKTATSKTLAPASLEKKAEESSTSSDEDLPSSQVIKTPLIFVDPNRSPAGPAATPAQAQATGTLRKAQALESTARSSSESEDEDVIPATQCPTPATRTSVVTVPTAPPKAALRASVVGAPSSEDTSQGSEGKKQEASATQVTKRNLPLTQAALKVLAQKASEAQSPAAKTPSSSGTDSAVGTLSTASPQSTPTQVRMANKLRKPEVPTAQQATATPSGHPTAKAPATSDDSNSSSSGSEDDAKGSQAAPSAHRPGPAPSRRETLVEETTTESSDDEVVAPSQSLLSGFVTPGLTPAGSKTSKAAPKPDASPSVSSTPATRDAPEGKQEVEPQQAAGTMSPKTGRKEAGATPQKPGKGLGSPPASMLALQSNITQRLLSEPWPLSEAQVQASVAKVLTELLEQERKKAMDTAKEGSRKGRLGHKRKLSEDQTAPKAPKNKKKKQLAAADGGEHVVSSEKAPRTVKGKSKKDRASGDVKEKKEKESPGSQGAKEKPVGELGTPKGDGGDHGNLKMKKEKKKSDKKKKDKEKKEKKKKAKKASTKDPGSPSQKKKKRKKKTAEQTV

Target ORF information:

RefSeq Version	XM_010798512.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010798512.2

ORF Insert Sequence:

ATGGCTGAGG CCAGGAAGCG GCGAGAGCTC CTTCCCCTGA TCTACCAGCA TCTGCTGCAG 
GCGGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA AATTTTCCTG 
AGTCAGCCTG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACTTC GGAGATTGGC 
CGGAAACGGA AGGCAGAGGA AGATGCGGCC TTGCAGGCCA AGAGGAGCCG TGTGTCAGAC 
CCCATCAGCA GCTCAGAAAG CTCAGAAGAG GAGGAGGAAG AGGAGGAGGC AGAAGCCAAA 
ACCACCAAAC CCACCCCGAG ATTAGCAGCT ACCAACTCCT CAGTCACAGG GACAGTTTCG 
TCTTCAAATG TGAAAGAAAA AGCCAAGGCA AAGACCACGA AAGCCAGCAA GACGGTGAAC 
TCCACGACAC ACCCTGCCCC GGGAAAGGCA GTGGCCCACC TCCTCACTGG GAAGTCACCC 
CAGAAGACAT CGGGGCCCTC GGCAAATGCT GTCTTGGTGT CAGAAACCGA GGAGGAGGGC 
AGCGTGTCAG CCCTCAGAAC CGCCACCACG CCTGGAATGG CATCCGCCGA CCAGGCTGAC 
AGCTCCAGTG AGGATACCAC CAGCTCGAGT GATGAGACGG ACTTGGAGGC GAAAGCCGCA 
GTAAAACCAC TGCAGGTCAG AGCCTCAGCC ACTGCAGTCA AGGAGTCTCC GAGAAAAAGT 
GCAGCCCCGA CTCCAGGGAA GGCAGGGGTT GTGACACCCC AAGTCAGAGG CGGTGCTGTG 
ACCCCAGCCA GCAGGGCCAA GAAGCCAGAA GAGGTCTCAG AGAGCAGTGA AGAGTCCGAC 
GAGAGTGAGG AGGCCGCCCC TGCGGGGACA CCCAGTCAGG TGAAGGCCTT GGAGAAAACC 
GTCCCAATCA GAGTTGCTCC TAGTCCTGCC AAGGGGACCC CTGGGAAAGG GGCCACCCCA 
GCACCCCCTG GGAAGGCAGG GCCCTTAGGG AAGCCAGAGG AGGACTCGGA GAGCAGCAGC 
GAGGAGTCAG ACAGCGAGGA GGAGATGCAG AAGGCAGGGA CCTCAGCCCA GGCACAGTCC 
TCTGGGAAAG CCCTCCAGAT CAGACCTGCC TCCGGTCCCA CCAAGAGGCC CCCTCAGAAG 
GCCGGGCCTG CAACCACCCA GGTCAAGGTT GAAAGGGCCG AGGAAGACTC GGAGAGCAGT 
GAGGAGGAAT CAGACAGTGA GGATGAAGCT CCTGTAGCCA AGACTCCAGC CCAGGCAAAA 
TCAGCTGTGA AAACTTCTCA GATCAAGGCC TCCCCAAGGA AGGGCACCCC CATTACACCC 
TCATCTGCCA GGGTCCCCCC AGTGCAAGTC GGCACACCAG CCCCCCGGAC AGCAGCAGCA 
GTGAGTACGC CCACCTGCGC GTCATCCCCA GCCATAGTCA GGCACACCCA GAAGCCAGGG 
GAGGACTCTT CAAGCAGCGA GGAGTCCGAG AGCGAGGAGG AGACAGCTCC TGCTGCAGCA 
GGGGGACAGA TGAAGTCTAT AGGGAAGACC CTCCCAGTGA AAGCTGCCTC ACCAGCCACC 
AAGGGGCCCT CAGGAAAAGG GACCACCCCA GGGCCCCCTG GGAAGGCAGG ACCTGCAGTG 
GCACAGGTCA AGGCTGAGGT GCAGGAAGAC TCAGAAAGCA GTGAGGAGTC GGACAATGAG 
GAAGCGGCTG CAACTCCCGC ACAGGTGAAG ACTTCAGTGA AAACCCCTCA GACCAAAGCC 
AACTCTTCTG CCACCAGAGT GGCTTCAGCC AAAGCAGCAG CACCAGCTCC TGGAAAGGGG 
GGCCTTGCTG TTGCTCAAGC CAAAGTGGGG TCCCCAGCCA AGGTAAAACC ACCAGCGAGA 
ACCCCCCAGA GTAGCGCCGT CTCTGGGAGG GGCCAGGTGT CTGTGCCGGC TGTGGGGAAG 
GCAGCAGCTG CAGCAGCCCA GGCCCAGCCG GGGCCTGTCA AGGGCTCACA GGAGGACTCG 
GAGAGCAGTG AGGAGGAGTC GGACAGTGAG GGAGAGGCAC CTCCTCAGGG GAAGCCCTCA 
GGAAAGACCC CCCAGGTCAG AACTGCCTCA GCCCCCACCA AGGCGTCCCC CAAGAAAGGG 
GCTGCCCCAG TACCCCCTGG GAAAGCAGGA CCCCCAGCCA CCCAGGCCAA GAGGCAGGAG 
GAGGACTCGG AGAGCAGCAG CGAGGAGGAG TCGGACAGTG ACGGGGACGT GCCAGCTGCT 
GCACCTCCAG CCCAGAAAGA GGGTAGCTCC AAAACTGCCA CAAGCAAGAC CCTGGCCCCA 
GCGTCCCTGG AGAAGAAAGC AGAGGAGTCC TCAACCAGCA GTGATGAGGA CCTGCCATCC 
AGCCAGGTGA TTAAAACCCC TCTGATTTTT GTCGACCCTA ATCGTAGTCC AGCTGGTCCA 
GCTGCTACCC CCGCCCAAGC GCAGGCTACA GGCACCCTGA GGAAGGCTCA GGCCTTGGAG 
AGCACAGCCA GGAGCTCCTC TGAGAGCGAG GACGAGGACG TGATCCCCGC TACACAGTGC 
CCGACTCCTG CCACTAGGAC CAGCGTGGTG ACTGTGCCCA CGGCACCCCC AAAGGCGGCC 
CTCAGAGCCA GTGTGGTGGG GGCTCCCAGC AGTGAGGACA CAAGTCAGGG GTCTGAAGGC 
AAGAAGCAGG AGGCCTCAGC CACTCAGGTG ACCAAGAGGA ACCTCCCGCT GACACAGGCT 
GCCCTGAAGG TTCTTGCCCA GAAAGCCAGT GAGGCCCAAT CTCCTGCTGC CAAGACCCCG 
TCTTCAAGTG GGACTGACAG TGCTGTGGGA ACACTCTCCA CGGCAAGTCC CCAGAGCACC 
CCTACCCAGG TCAGAATGGC CAACAAGCTC AGAAAGCCTG AGGTTCCTAC AGCTCAGCAG 
GCCACAGCCA CTCCCTCGGG ACACCCCACA GCCAAGGCCC CTGCAACCTC AGATGACAGC 
AACAGCAGCT CTTCAGGGAG CGAGGACGAT GCCAAGGGGT CCCAGGCGGC CCCGTCAGCC 
CACAGGCCAG GCCCAGCCCC CTCCAGGAGG GAGACCTTGG TGGAAGAGAC CACAACAGAG 
TCCAGTGACG ACGAGGTGGT GGCACCTTCC CAGTCTCTCC TCTCAGGTTT TGTGACTCCT 
GGGCTGACTC CAGCTGGTTC CAAGACTTCA AAGGCCGCTC CCAAACCAGA CGCCAGCCCC 
TCTGTTTCCT CTACTCCGGC CACCAGAGAT GCCCCAGAGG GCAAGCAGGA AGTGGAGCCC 
CAACAAGCAG CAGGCACCAT GTCCCCTAAA ACAGGCAGAA AAGAGGCTGG TGCCACACCT 
CAGAAGCCCG GGAAGGGGCT CGGGAGTCCC CCGGCCTCCA TGCTGGCGCT GCAGAGCAAC 
ATCACCCAGC GCCTCCTGAG CGAGCCCTGG CCCCTGAGCG AGGCGCAGGT GCAGGCCTCG 
GTGGCGAAGG TGCTCACGGA GCTGCTGGAG CAGGAGAGGA AGAAGGCCAT GGACACCGCC 
AAGGAAGGCA GCCGGAAGGG CCGCCTGGGC CATAAGCGGA AGCTGTCCGA AGACCAGACA 
GCCCCCAAGG CCCCCAAGAA CAAGAAAAAG AAGCAGCTGG CAGCTGCAGA TGGTGGGGAG 
CATGTCGTCT CCTCAGAAAA GGCCCCCAGG ACTGTCAAAG GGAAATCCAA GAAGGACAGA 
GCAAGTGGTG ACGTCAAGGA GAAGAAGGAG AAGGAGTCTC CCGGCTCTCA AGGGGCCAAG 
GAGAAGCCAG TAGGGGAGCT GGGGACTCCA AAGGGTGACG GGGGAGACCA CGGCAACCTT 
AAGATGAAGA AAGAGAAGAA GAAATCTGAC AAGAAAAAAA AAGACAAGGA GAAAAAGGAA 
AAGAAGAAGA AAGCAAAAAA GGCCTCAACC AAAGACCCTG GCTCACCATC ACAGAAGAAA 
AAGAAGAGAA AGAAGAAGAC AGCAGAGCAA ACCGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_015472323.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015327809.1 Bos taurus Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBa90352
Clone ID Related Accession (Same CDS sequence)	XM_015472323.1 , XM_015464334.1
Accession Version	XM_015472323.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	treacle protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104039.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAGG CCAGGAAGCG GCGAGAGCTC CTTCCCCTGA TCTACCAGCA TCTGCTGCAG 
GCGGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA AATTTTCCTG 
AGTCAGCCTG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACTTC GGAGATTGGC 
CGGAAACGGA AGGCAGAGGA AGATGCGGCC TTGCAGGCCA AGAGGAGCCG TGTGTCAGAC 
CCCATCAGCA GCTCAGAAAG CTCAGAAGAG GAGGAGGAAG AGGAGGAGGC AGAAGCCAAA 
ACCACCAAAC CCACCCCGAG ATTAGCAGCT ACCAACTCCT CAGTCACAGG GACAGTTTCG 
TCTTCAAATG TGAAAGAAAA AGCCAAGGCA AAGACCACGA AAGCCAGCAA GACGGTGAAC 
TCCACGACAC ACCCTGCCCC GGGAAAGGCA GTGGCCCACC TCCTCACTGG GAAGTCACCC 
CAGAAGACAT CGGGGCCCTC GGCAAATGCT GTCTTGGTGT CAGAAACCGA GGAGGAGGGC 
AGCGTGTCAG CCCTCAGAAC CGCCACCACG CCTGGAATGG CATCCGCCGA CCAGGCTGAC 
AGCTCCAGTG AGGATACCAC CAGCTCGAGT GATGAGACGG ACTTGGAGGC GAAAGCCGCA 
GTAAAACCAC TGCAGGTCAG AGCCTCAGCC ACTGCAGTCA AGGAGTCTCC GAGAAAAAGT 
GCAGCCCCGA CTCCAGGGAA GGCAGGGGTT GTGACACCCC AAGTCAGAGG CGGTGCTGTG 
ACCCCAGCCA GCAGGGCCAA GAAGCCAGAA GAGGTCTCAG AGAGCAGTGA AGAGTCCGAC 
GAGAGTGAGG AGGCCGCCCC TGCGGGGACA CCCAGTCAGG TGAAGGCCTT GGAGAAAACC 
GTCCCAATCA GAGTTGCTCC TAGTCCTGCC AAGGGGACCC CTGGGAAAGG GGCCACCCCA 
GCACCCCCTG GGAAGGCAGG GCCCTTAGGG AAGCCAGAGG AGGACTCGGA GAGCAGCAGC 
GAGGAGTCAG ACAGCGAGGA GGAGACACAA GCTGTCAAGA CCCCAGTGCA GGCAAAGCCC 
TCTGGGAAGA TCCCCCAGGT TAAAGCTGCC TCCACACCAA AAATCCAGGT GTTTTTCCCT 
AAGAAAGGGT CCCCTCCAAC ATCCCCTGGC AAGGCAGGGC CTGCAGCCAC CCAAGCCCAG 
CCCAGGAAGC AGGAGGAGGA GGAGAGCAGC AGCAGCAGCA GCAAGGAATC AGACAGCGAG 
GGCGAGGTGC CCACGGCCAT GCAGCAGACT ACAAGCCTGG CTCAGGCAAA GCCCTTGGAG 
AAAAACTCTC AGGTCAGAGC TGCCTCAGCC ACAGGTGCAG GACCCTCGGC AAAGGGCACG 
GTCCCAGCGC TCCCTCAGAA GGCAAGGTCT GTGGCCGCCC AGGATGGGAA GCAGGAGGAC 
TCAGAGAGCA GCAGTGAGGA GGAGTCAGAC AGCGAGGAGG AGATGCAGAA GGCAGGGACC 
TCAGCCCAGG CACAGTCCTC TGGGAAAGCC CTCCAGATCA GACCTGCCTC CGGTCCCACC 
AAGAGGCCCC CTCAGAAGGC CGGGCCTGCA ACCACCCAGG TCAAGGTTGA AAGGGCCGAG 
GAAGACTCGG AGAGCAGTGA GGAGGAATCA GACAGTGAGG ATGAAGCTCC TGTAGCCAAG 
ACTCCAGCCC AGGCAAAATC AGCTGTGAAA ACTTCTCAGA TCAAGGCCTC CCCAAGGAAG 
GGCACCCCCA TTACACCCTC ATCTGCCAGG GTCCCCCCAG TGCAAGTCGG CACACCAGCC 
CCCCGGACAG CAGCAGCAGT GAGTACGCCC ACCTGCGCGT CATCCCCAGC CATAGTCAGG 
CACACCCAGA AGCCAGGGGA GGACTCTTCA AGCAGCGAGG AGTCCGAGAG CGAGGAGGAG 
ACAGCTCCTG CTGCAGCAGG GGGACAGATG AAGTCTATAG GGAAGACCCT CCCAGTGAAA 
GCTGCCTCAC CAGCCACCAA GGGGCCCTCA GGAAAAGGGA CCACCCCAGG GCCCCCTGGG 
AAGGCAGGAC CTGCAGTGGC ACAGGTCAAG GCTGAGGTGC AGGAAGACTC AGAAAGCAGT 
GAGGAGTCGG ACAATGAGGA AGCGGCTGCA ACTCCCGCAC AGGTGAAGAC TTCAGTGAAA 
ACCCCTCAGA CCAAAGCCAA CTCTTCTGCC ACCAGAGTGG CTTCAGCCAA AGCAGCAGCA 
CCAGCTCCTG GAAAGGGGGG CCTTGCTGTT GCTCAAGCCA AAGTGGGGTC CCCAGCCAAG 
GTAAAACCAC CAGCGAGAAC CCCCCAGAGT AGCGCCGTCT CTGGGAGGGG CCAGGTGTCT 
GTGCCGGCTG TGGGGAAGGC AGCAGCTGCA GCAGCCCAGG CCCAGCCGGG GCCTGTCAAG 
GGCTCACAGG AGGACTCGGA GAGCAGTGAG GAGGAGTCGG ACAGTGAGGG AGAGGCACCT 
CCTCAGGGGA AGCCCTCAGG AAAGACCCCC CAGGTCAGAA CTGCCTCAGC CCCCACCAAG 
GCGTCCCCCA AGAAAGGGGC TGCCCCAGTA CCCCCTGGGA AAGCAGGACC CCCAGCCACC 
CAGGCCAAGA GGCAGGAGGA GGACTCGGAG AGCAGCAGCG AGGAGGAGTC GGACAGTGAC 
GGGGACGTGC CAGCTGCTGC ACCTCCAGCC CAGAAAGAGG GTAGCTCCAA AACTGCCACA 
AGCAAGACCC TGGCCCCAGC GTCCCTGGAG AAGAAAGCAG AGGAGTCCTC AACCAGCAGT 
GATGAGGACC TGCCATCCAG CCAGGTGATT AAAACCCCTC TGATTTTTGT CGACCCTAAT 
CGTAGTCCAG CTGGTCCAGC TGCTACCCCC GCCCAAGCGC AGGCTACAGG CACCCTGAGG 
AAGGCTCAGG CCTTGGAGAG CACAGCCAGG AGCTCCTCTG AGAGCGAGGA CGAGGACGTG 
ATCCCCGCTA CACAGTGCCC GACTCCTGCC ACTAGGACCA GCGTGGTGAC TGTGCCCACG 
GCACCCCCAA AGGCGGCCCT CAGAGCCAGT GTGGTGGGGG CTCCCAGCAG TGAGGACACA 
AGTCAGGGGT CTGAAGGCAA GAAGCAGGAG GCCTCAGCCA CTCAGGTGAC CAAGAGGAAC 
CTCCCGCTGA CACAGGCTGC CCTGAAGGTT CTTGCCCAGA AAGCCAGTGA GGCCCAATCT 
CCTGCTGCCA AGACCCCGTC TTCAAGTGGG ACTGACAGTG CTGTGGGAAC ACTCTCCACG 
GCAAGTCCCC AGAGCACCCC TACCCAGGTC AGAATGGCCA ACAAGCTCAG AAAGCCTGAG 
GTTCCTACAG CTCAGCAGGC CACAGCCACT CCCTCGGGAC ACCCCACAGC CAAGGCCCCT 
GCAACCTCAG ATGACAGCAA CAGCAGCTCT TCAGGGAGCG AGGACGATGC CAAGGGGTCC 
CAGGCGGCCC CGTCAGCCCA CAGGCCAGGC CCAGCCCCCT CCAGGAGGGA GACCTTGGTG 
GAAGAGACCA CAACAGAGTC CAGTGACGAC GAGGTGGTGG CACCTTCCCA GGTTTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015327809.1
CDS	59..3598
Translation	MAEARKRRELLPLIYQHLLQAGYVRAAREVKEQSGQKIFLSQPVTLLDIYTHWQQTSEIGRKRKAEEDAALQAKRSRVSDPISSSESSEEEEEEEEAEAKTTKPTPRLAATNSSVTGTVSSSNVKEKAKAKTTKASKTVNSTTHPAPGKAVAHLLTGKSPQKTSGPSANAVLVSETEEEGSVSALRTATTPGMASADQADSSSEDTTSSSDETDLEAKAAVKPLQVRASATAVKESPRKSAAPTPGKAGVVTPQVRGGAVTPASRAKKPEEVSESSEESDESEEAAPAGTPSQVKALEKTVPIRVAPSPAKGTPGKGATPAPPGKAGPLGKPEEDSESSSEESDSEEETQAVKTPVQAKPSGKIPQVKAASTPKIQVFFPKKGSPPTSPGKAGPAATQAQPRKQEEEESSSSSSKESDSEGEVPTAMQQTTSLAQAKPLEKNSQVRAASATGAGPSAKGTVPALPQKARSVAAQDGKQEDSESSSEEESDSEEEMQKAGTSAQAQSSGKALQIRPASGPTKRPPQKAGPATTQVKVERAEEDSESSEEESDSEDEAPVAKTPAQAKSAVKTSQIKASPRKGTPITPSSARVPPVQVGTPAPRTAAAVSTPTCASSPAIVRHTQKPGEDSSSSEESESEEETAPAAAGGQMKSIGKTLPVKAASPATKGPSGKGTTPGPPGKAGPAVAQVKAEVQEDSESSEESDNEEAAATPAQVKTSVKTPQTKANSSATRVASAKAAAPAPGKGGLAVAQAKVGSPAKVKPPARTPQSSAVSGRGQVSVPAVGKAAAAAAQAQPGPVKGSQEDSESSEEESDSEGEAPPQGKPSGKTPQVRTASAPTKASPKKGAAPVPPGKAGPPATQAKRQEEDSESSSEEESDSDGDVPAAAPPAQKEGSSKTATSKTLAPASLEKKAEESSTSSDEDLPSSQVIKTPLIFVDPNRSPAGPAATPAQAQATGTLRKAQALESTARSSSESEDEDVIPATQCPTPATRTSVVTVPTAPPKAALRASVVGAPSSEDTSQGSEGKKQEASATQVTKRNLPLTQAALKVLAQKASEAQSPAAKTPSSSGTDSAVGTLSTASPQSTPTQVRMANKLRKPEVPTAQQATATPSGHPTAKAPATSDDSNSSSSGSEDDAKGSQAAPSAHRPGPAPSRRETLVEETTTESSDDEVVAPSQVL

Target ORF information:

RefSeq Version	XM_015472323.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015472323.1

ORF Insert Sequence:

ATGGCTGAGG CCAGGAAGCG GCGAGAGCTC CTTCCCCTGA TCTACCAGCA TCTGCTGCAG 
GCGGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA AATTTTCCTG 
AGTCAGCCTG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACTTC GGAGATTGGC 
CGGAAACGGA AGGCAGAGGA AGATGCGGCC TTGCAGGCCA AGAGGAGCCG TGTGTCAGAC 
CCCATCAGCA GCTCAGAAAG CTCAGAAGAG GAGGAGGAAG AGGAGGAGGC AGAAGCCAAA 
ACCACCAAAC CCACCCCGAG ATTAGCAGCT ACCAACTCCT CAGTCACAGG GACAGTTTCG 
TCTTCAAATG TGAAAGAAAA AGCCAAGGCA AAGACCACGA AAGCCAGCAA GACGGTGAAC 
TCCACGACAC ACCCTGCCCC GGGAAAGGCA GTGGCCCACC TCCTCACTGG GAAGTCACCC 
CAGAAGACAT CGGGGCCCTC GGCAAATGCT GTCTTGGTGT CAGAAACCGA GGAGGAGGGC 
AGCGTGTCAG CCCTCAGAAC CGCCACCACG CCTGGAATGG CATCCGCCGA CCAGGCTGAC 
AGCTCCAGTG AGGATACCAC CAGCTCGAGT GATGAGACGG ACTTGGAGGC GAAAGCCGCA 
GTAAAACCAC TGCAGGTCAG AGCCTCAGCC ACTGCAGTCA AGGAGTCTCC GAGAAAAAGT 
GCAGCCCCGA CTCCAGGGAA GGCAGGGGTT GTGACACCCC AAGTCAGAGG CGGTGCTGTG 
ACCCCAGCCA GCAGGGCCAA GAAGCCAGAA GAGGTCTCAG AGAGCAGTGA AGAGTCCGAC 
GAGAGTGAGG AGGCCGCCCC TGCGGGGACA CCCAGTCAGG TGAAGGCCTT GGAGAAAACC 
GTCCCAATCA GAGTTGCTCC TAGTCCTGCC AAGGGGACCC CTGGGAAAGG GGCCACCCCA 
GCACCCCCTG GGAAGGCAGG GCCCTTAGGG AAGCCAGAGG AGGACTCGGA GAGCAGCAGC 
GAGGAGTCAG ACAGCGAGGA GGAGACACAA GCTGTCAAGA CCCCAGTGCA GGCAAAGCCC 
TCTGGGAAGA TCCCCCAGGT TAAAGCTGCC TCCACACCAA AAATCCAGGT GTTTTTCCCT 
AAGAAAGGGT CCCCTCCAAC ATCCCCTGGC AAGGCAGGGC CTGCAGCCAC CCAAGCCCAG 
CCCAGGAAGC AGGAGGAGGA GGAGAGCAGC AGCAGCAGCA GCAAGGAATC AGACAGCGAG 
GGCGAGGTGC CCACGGCCAT GCAGCAGACT ACAAGCCTGG CTCAGGCAAA GCCCTTGGAG 
AAAAACTCTC AGGTCAGAGC TGCCTCAGCC ACAGGTGCAG GACCCTCGGC AAAGGGCACG 
GTCCCAGCGC TCCCTCAGAA GGCAAGGTCT GTGGCCGCCC AGGATGGGAA GCAGGAGGAC 
TCAGAGAGCA GCAGTGAGGA GGAGTCAGAC AGCGAGGAGG AGATGCAGAA GGCAGGGACC 
TCAGCCCAGG CACAGTCCTC TGGGAAAGCC CTCCAGATCA GACCTGCCTC CGGTCCCACC 
AAGAGGCCCC CTCAGAAGGC CGGGCCTGCA ACCACCCAGG TCAAGGTTGA AAGGGCCGAG 
GAAGACTCGG AGAGCAGTGA GGAGGAATCA GACAGTGAGG ATGAAGCTCC TGTAGCCAAG 
ACTCCAGCCC AGGCAAAATC AGCTGTGAAA ACTTCTCAGA TCAAGGCCTC CCCAAGGAAG 
GGCACCCCCA TTACACCCTC ATCTGCCAGG GTCCCCCCAG TGCAAGTCGG CACACCAGCC 
CCCCGGACAG CAGCAGCAGT GAGTACGCCC ACCTGCGCGT CATCCCCAGC CATAGTCAGG 
CACACCCAGA AGCCAGGGGA GGACTCTTCA AGCAGCGAGG AGTCCGAGAG CGAGGAGGAG 
ACAGCTCCTG CTGCAGCAGG GGGACAGATG AAGTCTATAG GGAAGACCCT CCCAGTGAAA 
GCTGCCTCAC CAGCCACCAA GGGGCCCTCA GGAAAAGGGA CCACCCCAGG GCCCCCTGGG 
AAGGCAGGAC CTGCAGTGGC ACAGGTCAAG GCTGAGGTGC AGGAAGACTC AGAAAGCAGT 
GAGGAGTCGG ACAATGAGGA AGCGGCTGCA ACTCCCGCAC AGGTGAAGAC TTCAGTGAAA 
ACCCCTCAGA CCAAAGCCAA CTCTTCTGCC ACCAGAGTGG CTTCAGCCAA AGCAGCAGCA 
CCAGCTCCTG GAAAGGGGGG CCTTGCTGTT GCTCAAGCCA AAGTGGGGTC CCCAGCCAAG 
GTAAAACCAC CAGCGAGAAC CCCCCAGAGT AGCGCCGTCT CTGGGAGGGG CCAGGTGTCT 
GTGCCGGCTG TGGGGAAGGC AGCAGCTGCA GCAGCCCAGG CCCAGCCGGG GCCTGTCAAG 
GGCTCACAGG AGGACTCGGA GAGCAGTGAG GAGGAGTCGG ACAGTGAGGG AGAGGCACCT 
CCTCAGGGGA AGCCCTCAGG AAAGACCCCC CAGGTCAGAA CTGCCTCAGC CCCCACCAAG 
GCGTCCCCCA AGAAAGGGGC TGCCCCAGTA CCCCCTGGGA AAGCAGGACC CCCAGCCACC 
CAGGCCAAGA GGCAGGAGGA GGACTCGGAG AGCAGCAGCG AGGAGGAGTC GGACAGTGAC 
GGGGACGTGC CAGCTGCTGC ACCTCCAGCC CAGAAAGAGG GTAGCTCCAA AACTGCCACA 
AGCAAGACCC TGGCCCCAGC GTCCCTGGAG AAGAAAGCAG AGGAGTCCTC AACCAGCAGT 
GATGAGGACC TGCCATCCAG CCAGGTGATT AAAACCCCTC TGATTTTTGT CGACCCTAAT 
CGTAGTCCAG CTGGTCCAGC TGCTACCCCC GCCCAAGCGC AGGCTACAGG CACCCTGAGG 
AAGGCTCAGG CCTTGGAGAG CACAGCCAGG AGCTCCTCTG AGAGCGAGGA CGAGGACGTG 
ATCCCCGCTA CACAGTGCCC GACTCCTGCC ACTAGGACCA GCGTGGTGAC TGTGCCCACG 
GCACCCCCAA AGGCGGCCCT CAGAGCCAGT GTGGTGGGGG CTCCCAGCAG TGAGGACACA 
AGTCAGGGGT CTGAAGGCAA GAAGCAGGAG GCCTCAGCCA CTCAGGTGAC CAAGAGGAAC 
CTCCCGCTGA CACAGGCTGC CCTGAAGGTT CTTGCCCAGA AAGCCAGTGA GGCCCAATCT 
CCTGCTGCCA AGACCCCGTC TTCAAGTGGG ACTGACAGTG CTGTGGGAAC ACTCTCCACG 
GCAAGTCCCC AGAGCACCCC TACCCAGGTC AGAATGGCCA ACAAGCTCAG AAAGCCTGAG 
GTTCCTACAG CTCAGCAGGC CACAGCCACT CCCTCGGGAC ACCCCACAGC CAAGGCCCCT 
GCAACCTCAG ATGACAGCAA CAGCAGCTCT TCAGGGAGCG AGGACGATGC CAAGGGGTCC 
CAGGCGGCCC CGTCAGCCCA CAGGCCAGGC CCAGCCCCCT CCAGGAGGGA GACCTTGGTG 
GAAGAGACCA CAACAGAGTC CAGTGACGAC GAGGTGGTGG CACCTTCCCA GGTTTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_015464334.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319820.1 Bos taurus Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant X8, mRNA.

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CloneID	OBa90352
Clone ID Related Accession (Same CDS sequence)	XM_015472323.1 , XM_015464334.1
Accession Version	XM_015464334.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	treacle protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645112.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAGG CCAGGAAGCG GCGAGAGCTC CTTCCCCTGA TCTACCAGCA TCTGCTGCAG 
GCGGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA AATTTTCCTG 
AGTCAGCCTG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACTTC GGAGATTGGC 
CGGAAACGGA AGGCAGAGGA AGATGCGGCC TTGCAGGCCA AGAGGAGCCG TGTGTCAGAC 
CCCATCAGCA GCTCAGAAAG CTCAGAAGAG GAGGAGGAAG AGGAGGAGGC AGAAGCCAAA 
ACCACCAAAC CCACCCCGAG ATTAGCAGCT ACCAACTCCT CAGTCACAGG GACAGTTTCG 
TCTTCAAATG TGAAAGAAAA AGCCAAGGCA AAGACCACGA AAGCCAGCAA GACGGTGAAC 
TCCACGACAC ACCCTGCCCC GGGAAAGGCA GTGGCCCACC TCCTCACTGG GAAGTCACCC 
CAGAAGACAT CGGGGCCCTC GGCAAATGCT GTCTTGGTGT CAGAAACCGA GGAGGAGGGC 
AGCGTGTCAG CCCTCAGAAC CGCCACCACG CCTGGAATGG CATCCGCCGA CCAGGCTGAC 
AGCTCCAGTG AGGATACCAC CAGCTCGAGT GATGAGACGG ACTTGGAGGC GAAAGCCGCA 
GTAAAACCAC TGCAGGTCAG AGCCTCAGCC ACTGCAGTCA AGGAGTCTCC GAGAAAAAGT 
GCAGCCCCGA CTCCAGGGAA GGCAGGGGTT GTGACACCCC AAGTCAGAGG CGGTGCTGTG 
ACCCCAGCCA GCAGGGCCAA GAAGCCAGAA GAGGTCTCAG AGAGCAGTGA AGAGTCCGAC 
GAGAGTGAGG AGGCCGCCCC TGCGGGGACA CCCAGTCAGG TGAAGGCCTT GGAGAAAACC 
GTCCCAATCA GAGTTGCTCC TAGTCCTGCC AAGGGGACCC CTGGGAAAGG GGCCACCCCA 
GCACCCCCTG GGAAGGCAGG GCCCTTAGGG AAGCCAGAGG AGGACTCGGA GAGCAGCAGC 
GAGGAGTCAG ACAGCGAGGA GGAGACACAA GCTGTCAAGA CCCCAGTGCA GGCAAAGCCC 
TCTGGGAAGA TCCCCCAGGT TAAAGCTGCC TCCACACCAA AAATCCAGGT GTTTTTCCCT 
AAGAAAGGGT CCCCTCCAAC ATCCCCTGGC AAGGCAGGGC CTGCAGCCAC CCAAGCCCAG 
CCCAGGAAGC AGGAGGAGGA GGAGAGCAGC AGCAGCAGCA GCAAGGAATC AGACAGCGAG 
GGCGAGGTGC CCACGGCCAT GCAGCAGACT ACAAGCCTGG CTCAGGCAAA GCCCTTGGAG 
AAAAACTCTC AGGTCAGAGC TGCCTCAGCC ACAGGTGCAG GACCCTCGGC AAAGGGCACG 
GTCCCAGCGC TCCCTCAGAA GGCAAGGTCT GTGGCCGCCC AGGATGGGAA GCAGGAGGAC 
TCAGAGAGCA GCAGTGAGGA GGAGTCAGAC AGCGAGGAGG AGATGCAGAA GGCAGGGACC 
TCAGCCCAGG CACAGTCCTC TGGGAAAGCC CTCCAGATCA GACCTGCCTC CGGTCCCACC 
AAGAGGCCCC CTCAGAAGGC CGGGCCTGCA ACCACCCAGG TCAAGGTTGA AAGGGCCGAG 
GAAGACTCGG AGAGCAGTGA GGAGGAATCA GACAGTGAGG ATGAAGCTCC TGTAGCCAAG 
ACTCCAGCCC AGGCAAAATC AGCTGTGAAA ACTTCTCAGA TCAAGGCCTC CCCAAGGAAG 
GGCACCCCCA TTACACCCTC ATCTGCCAGG GTCCCCCCAG TGCAAGTCGG CACACCAGCC 
CCCCGGACAG CAGCAGCAGT GAGTACGCCC ACCTGCGCGT CATCCCCAGC CATAGTCAGG 
CACACCCAGA AGCCAGGGGA GGACTCTTCA AGCAGCGAGG AGTCCGAGAG CGAGGAGGAG 
ACAGCTCCTG CTGCAGCAGG GGGACAGATG AAGTCTATAG GGAAGACCCT CCCAGTGAAA 
GCTGCCTCAC CAGCCACCAA GGGGCCCTCA GGAAAAGGGA CCACCCCAGG GCCCCCTGGG 
AAGGCAGGAC CTGCAGTGGC ACAGGTCAAG GCTGAGGTGC AGGAAGACTC AGAAAGCAGT 
GAGGAGTCGG ACAATGAGGA AGCGGCTGCA ACTCCCGCAC AGGTGAAGAC TTCAGTGAAA 
ACCCCTCAGA CCAAAGCCAA CTCTTCTGCC ACCAGAGTGG CTTCAGCCAA AGCAGCAGCA 
CCAGCTCCTG GAAAGGGGGG CCTTGCTGTT GCTCAAGCCA AAGTGGGGTC CCCAGCCAAG 
GTAAAACCAC CAGCGAGAAC CCCCCAGAGT AGCGCCGTCT CTGGGAGGGG CCAGGTGTCT 
GTGCCGGCTG TGGGGAAGGC AGCAGCTGCA GCAGCCCAGG CCCAGCCGGG GCCTGTCAAG 
GGCTCACAGG AGGACTCGGA GAGCAGTGAG GAGGAGTCGG ACAGTGAGGG AGAGGCACCT 
CCTCAGGGGA AGCCCTCAGG AAAGACCCCC CAGGTCAGAA CTGCCTCAGC CCCCACCAAG 
GCGTCCCCCA AGAAAGGGGC TGCCCCAGTA CCCCCTGGGA AAGCAGGACC CCCAGCCACC 
CAGGCCAAGA GGCAGGAGGA GGACTCGGAG AGCAGCAGCG AGGAGGAGTC GGACAGTGAC 
GGGGACGTGC CAGCTGCTGC ACCTCCAGCC CAGAAAGAGG GTAGCTCCAA AACTGCCACA 
AGCAAGACCC TGGCCCCAGC GTCCCTGGAG AAGAAAGCAG AGGAGTCCTC AACCAGCAGT 
GATGAGGACC TGCCATCCAG CCAGGTGATT AAAACCCCTC TGATTTTTGT CGACCCTAAT 
CGTAGTCCAG CTGGTCCAGC TGCTACCCCC GCCCAAGCGC AGGCTACAGG CACCCTGAGG 
AAGGCTCAGG CCTTGGAGAG CACAGCCAGG AGCTCCTCTG AGAGCGAGGA CGAGGACGTG 
ATCCCCGCTA CACAGTGCCC GACTCCTGCC ACTAGGACCA GCGTGGTGAC TGTGCCCACG 
GCACCCCCAA AGGCGGCCCT CAGAGCCAGT GTGGTGGGGG CTCCCAGCAG TGAGGACACA 
AGTCAGGGGT CTGAAGGCAA GAAGCAGGAG GCCTCAGCCA CTCAGGTGAC CAAGAGGAAC 
CTCCCGCTGA CACAGGCTGC CCTGAAGGTT CTTGCCCAGA AAGCCAGTGA GGCCCAATCT 
CCTGCTGCCA AGACCCCGTC TTCAAGTGGG ACTGACAGTG CTGTGGGAAC ACTCTCCACG 
GCAAGTCCCC AGAGCACCCC TACCCAGGTC AGAATGGCCA ACAAGCTCAG AAAGCCTGAG 
GTTCCTACAG CTCAGCAGGC CACAGCCACT CCCTCGGGAC ACCCCACAGC CAAGGCCCCT 
GCAACCTCAG ATGACAGCAA CAGCAGCTCT TCAGGGAGCG AGGACGATGC CAAGGGGTCC 
CAGGCGGCCC CGTCAGCCCA CAGGCCAGGC CCAGCCCCCT CCAGGAGGGA GACCTTGGTG 
GAAGAGACCA CAACAGAGTC CAGTGACGAC GAGGTGGTGG CACCTTCCCA GGTTTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319820.1
CDS	59..3598
Translation	MAEARKRRELLPLIYQHLLQAGYVRAAREVKEQSGQKIFLSQPVTLLDIYTHWQQTSEIGRKRKAEEDAALQAKRSRVSDPISSSESSEEEEEEEEAEAKTTKPTPRLAATNSSVTGTVSSSNVKEKAKAKTTKASKTVNSTTHPAPGKAVAHLLTGKSPQKTSGPSANAVLVSETEEEGSVSALRTATTPGMASADQADSSSEDTTSSSDETDLEAKAAVKPLQVRASATAVKESPRKSAAPTPGKAGVVTPQVRGGAVTPASRAKKPEEVSESSEESDESEEAAPAGTPSQVKALEKTVPIRVAPSPAKGTPGKGATPAPPGKAGPLGKPEEDSESSSEESDSEEETQAVKTPVQAKPSGKIPQVKAASTPKIQVFFPKKGSPPTSPGKAGPAATQAQPRKQEEEESSSSSSKESDSEGEVPTAMQQTTSLAQAKPLEKNSQVRAASATGAGPSAKGTVPALPQKARSVAAQDGKQEDSESSSEEESDSEEEMQKAGTSAQAQSSGKALQIRPASGPTKRPPQKAGPATTQVKVERAEEDSESSEEESDSEDEAPVAKTPAQAKSAVKTSQIKASPRKGTPITPSSARVPPVQVGTPAPRTAAAVSTPTCASSPAIVRHTQKPGEDSSSSEESESEEETAPAAAGGQMKSIGKTLPVKAASPATKGPSGKGTTPGPPGKAGPAVAQVKAEVQEDSESSEESDNEEAAATPAQVKTSVKTPQTKANSSATRVASAKAAAPAPGKGGLAVAQAKVGSPAKVKPPARTPQSSAVSGRGQVSVPAVGKAAAAAAQAQPGPVKGSQEDSESSEEESDSEGEAPPQGKPSGKTPQVRTASAPTKASPKKGAAPVPPGKAGPPATQAKRQEEDSESSSEEESDSDGDVPAAAPPAQKEGSSKTATSKTLAPASLEKKAEESSTSSDEDLPSSQVIKTPLIFVDPNRSPAGPAATPAQAQATGTLRKAQALESTARSSSESEDEDVIPATQCPTPATRTSVVTVPTAPPKAALRASVVGAPSSEDTSQGSEGKKQEASATQVTKRNLPLTQAALKVLAQKASEAQSPAAKTPSSSGTDSAVGTLSTASPQSTPTQVRMANKLRKPEVPTAQQATATPSGHPTAKAPATSDDSNSSSSGSEDDAKGSQAAPSAHRPGPAPSRRETLVEETTTESSDDEVVAPSQVL

Target ORF information:

RefSeq Version	XM_015464334.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015464334.1

ORF Insert Sequence:

ATGGCTGAGG CCAGGAAGCG GCGAGAGCTC CTTCCCCTGA TCTACCAGCA TCTGCTGCAG 
GCGGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA AATTTTCCTG 
AGTCAGCCTG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACTTC GGAGATTGGC 
CGGAAACGGA AGGCAGAGGA AGATGCGGCC TTGCAGGCCA AGAGGAGCCG TGTGTCAGAC 
CCCATCAGCA GCTCAGAAAG CTCAGAAGAG GAGGAGGAAG AGGAGGAGGC AGAAGCCAAA 
ACCACCAAAC CCACCCCGAG ATTAGCAGCT ACCAACTCCT CAGTCACAGG GACAGTTTCG 
TCTTCAAATG TGAAAGAAAA AGCCAAGGCA AAGACCACGA AAGCCAGCAA GACGGTGAAC 
TCCACGACAC ACCCTGCCCC GGGAAAGGCA GTGGCCCACC TCCTCACTGG GAAGTCACCC 
CAGAAGACAT CGGGGCCCTC GGCAAATGCT GTCTTGGTGT CAGAAACCGA GGAGGAGGGC 
AGCGTGTCAG CCCTCAGAAC CGCCACCACG CCTGGAATGG CATCCGCCGA CCAGGCTGAC 
AGCTCCAGTG AGGATACCAC CAGCTCGAGT GATGAGACGG ACTTGGAGGC GAAAGCCGCA 
GTAAAACCAC TGCAGGTCAG AGCCTCAGCC ACTGCAGTCA AGGAGTCTCC GAGAAAAAGT 
GCAGCCCCGA CTCCAGGGAA GGCAGGGGTT GTGACACCCC AAGTCAGAGG CGGTGCTGTG 
ACCCCAGCCA GCAGGGCCAA GAAGCCAGAA GAGGTCTCAG AGAGCAGTGA AGAGTCCGAC 
GAGAGTGAGG AGGCCGCCCC TGCGGGGACA CCCAGTCAGG TGAAGGCCTT GGAGAAAACC 
GTCCCAATCA GAGTTGCTCC TAGTCCTGCC AAGGGGACCC CTGGGAAAGG GGCCACCCCA 
GCACCCCCTG GGAAGGCAGG GCCCTTAGGG AAGCCAGAGG AGGACTCGGA GAGCAGCAGC 
GAGGAGTCAG ACAGCGAGGA GGAGACACAA GCTGTCAAGA CCCCAGTGCA GGCAAAGCCC 
TCTGGGAAGA TCCCCCAGGT TAAAGCTGCC TCCACACCAA AAATCCAGGT GTTTTTCCCT 
AAGAAAGGGT CCCCTCCAAC ATCCCCTGGC AAGGCAGGGC CTGCAGCCAC CCAAGCCCAG 
CCCAGGAAGC AGGAGGAGGA GGAGAGCAGC AGCAGCAGCA GCAAGGAATC AGACAGCGAG 
GGCGAGGTGC CCACGGCCAT GCAGCAGACT ACAAGCCTGG CTCAGGCAAA GCCCTTGGAG 
AAAAACTCTC AGGTCAGAGC TGCCTCAGCC ACAGGTGCAG GACCCTCGGC AAAGGGCACG 
GTCCCAGCGC TCCCTCAGAA GGCAAGGTCT GTGGCCGCCC AGGATGGGAA GCAGGAGGAC 
TCAGAGAGCA GCAGTGAGGA GGAGTCAGAC AGCGAGGAGG AGATGCAGAA GGCAGGGACC 
TCAGCCCAGG CACAGTCCTC TGGGAAAGCC CTCCAGATCA GACCTGCCTC CGGTCCCACC 
AAGAGGCCCC CTCAGAAGGC CGGGCCTGCA ACCACCCAGG TCAAGGTTGA AAGGGCCGAG 
GAAGACTCGG AGAGCAGTGA GGAGGAATCA GACAGTGAGG ATGAAGCTCC TGTAGCCAAG 
ACTCCAGCCC AGGCAAAATC AGCTGTGAAA ACTTCTCAGA TCAAGGCCTC CCCAAGGAAG 
GGCACCCCCA TTACACCCTC ATCTGCCAGG GTCCCCCCAG TGCAAGTCGG CACACCAGCC 
CCCCGGACAG CAGCAGCAGT GAGTACGCCC ACCTGCGCGT CATCCCCAGC CATAGTCAGG 
CACACCCAGA AGCCAGGGGA GGACTCTTCA AGCAGCGAGG AGTCCGAGAG CGAGGAGGAG 
ACAGCTCCTG CTGCAGCAGG GGGACAGATG AAGTCTATAG GGAAGACCCT CCCAGTGAAA 
GCTGCCTCAC CAGCCACCAA GGGGCCCTCA GGAAAAGGGA CCACCCCAGG GCCCCCTGGG 
AAGGCAGGAC CTGCAGTGGC ACAGGTCAAG GCTGAGGTGC AGGAAGACTC AGAAAGCAGT 
GAGGAGTCGG ACAATGAGGA AGCGGCTGCA ACTCCCGCAC AGGTGAAGAC TTCAGTGAAA 
ACCCCTCAGA CCAAAGCCAA CTCTTCTGCC ACCAGAGTGG CTTCAGCCAA AGCAGCAGCA 
CCAGCTCCTG GAAAGGGGGG CCTTGCTGTT GCTCAAGCCA AAGTGGGGTC CCCAGCCAAG 
GTAAAACCAC CAGCGAGAAC CCCCCAGAGT AGCGCCGTCT CTGGGAGGGG CCAGGTGTCT 
GTGCCGGCTG TGGGGAAGGC AGCAGCTGCA GCAGCCCAGG CCCAGCCGGG GCCTGTCAAG 
GGCTCACAGG AGGACTCGGA GAGCAGTGAG GAGGAGTCGG ACAGTGAGGG AGAGGCACCT 
CCTCAGGGGA AGCCCTCAGG AAAGACCCCC CAGGTCAGAA CTGCCTCAGC CCCCACCAAG 
GCGTCCCCCA AGAAAGGGGC TGCCCCAGTA CCCCCTGGGA AAGCAGGACC CCCAGCCACC 
CAGGCCAAGA GGCAGGAGGA GGACTCGGAG AGCAGCAGCG AGGAGGAGTC GGACAGTGAC 
GGGGACGTGC CAGCTGCTGC ACCTCCAGCC CAGAAAGAGG GTAGCTCCAA AACTGCCACA 
AGCAAGACCC TGGCCCCAGC GTCCCTGGAG AAGAAAGCAG AGGAGTCCTC AACCAGCAGT 
GATGAGGACC TGCCATCCAG CCAGGTGATT AAAACCCCTC TGATTTTTGT CGACCCTAAT 
CGTAGTCCAG CTGGTCCAGC TGCTACCCCC GCCCAAGCGC AGGCTACAGG CACCCTGAGG 
AAGGCTCAGG CCTTGGAGAG CACAGCCAGG AGCTCCTCTG AGAGCGAGGA CGAGGACGTG 
ATCCCCGCTA CACAGTGCCC GACTCCTGCC ACTAGGACCA GCGTGGTGAC TGTGCCCACG 
GCACCCCCAA AGGCGGCCCT CAGAGCCAGT GTGGTGGGGG CTCCCAGCAG TGAGGACACA 
AGTCAGGGGT CTGAAGGCAA GAAGCAGGAG GCCTCAGCCA CTCAGGTGAC CAAGAGGAAC 
CTCCCGCTGA CACAGGCTGC CCTGAAGGTT CTTGCCCAGA AAGCCAGTGA GGCCCAATCT 
CCTGCTGCCA AGACCCCGTC TTCAAGTGGG ACTGACAGTG CTGTGGGAAC ACTCTCCACG 
GCAAGTCCCC AGAGCACCCC TACCCAGGTC AGAATGGCCA ACAAGCTCAG AAAGCCTGAG 
GTTCCTACAG CTCAGCAGGC CACAGCCACT CCCTCGGGAC ACCCCACAGC CAAGGCCCCT 
GCAACCTCAG ATGACAGCAA CAGCAGCTCT TCAGGGAGCG AGGACGATGC CAAGGGGTCC 
CAGGCGGCCC CGTCAGCCCA CAGGCCAGGC CCAGCCCCCT CCAGGAGGGA GACCTTGGTG 
GAAGAGACCA CAACAGAGTC CAGTGACGAC GAGGTGGTGG CACCTTCCCA GGTTTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	TCOF1
Entrez Gene ID	513591
Full Name	treacle ribosome biogenesis factor 1
General protein information	Preferred Names treacle ribosome biogenesis factor 1 Names treacle protein
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Homo sapiens (human)	TCOF1	NP_001128717.1
Bos taurus (cattle)	TCOF1	XP_002689339.2
Rattus norvegicus (Norway rat)	Tcof1	NP_001099613.1
Canis lupus familiaris (dog)	TCOF1	NP_001003057.1
Pan troglodytes (chimpanzee)	TCOF1	XP_527074.4
Mus musculus (house mouse)	Tcof1	NP_001185913.1
	TCOF1	NP_001035041.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

TCOF1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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