Tcof1 cDNA ORF clone, Rattus norvegicus(Norway rat)

The following Tcof1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tcof1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ORa04484 NM_001106143.1
Latest version!
Rattus norvegicus treacle ribosome biogenesis factor 1 (Tcof1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $727.30
$1039.00
ORa44960 XM_017600890.1
Latest version!
Rattus norvegicus treacle ribosome biogenesis factor 1 (Tcof1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $727.30
$1039.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ORa04484
    Clone ID Related Accession (Same CDS sequence) NM_001106143.1
    Accession Version NM_001106143.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4188bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-21
    Organism Rattus norvegicus(Norway rat)
    Product treacle protein
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473971.1. On or before Oct 4, 2007 this sequence version replaced XM_001061846.1, XM_214552.4. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMN01906347 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCCGAGG CCAGGAAGCG GCGGGAGCTG CTTCCCCTTA TTTACCATCA TCTGTTGCAA 
    GCAGGCTACG TCCGCGCGGC GCGGGAAGTT AAGGAGCAGA GCGGCCAGAA GAATTTCCTG
    ACTCATCCCG TCACCCTTCT GGACATCTAT ACACACTGGC AACAGACCTC AGAGCTCGGC
    CTGAAGCAGA AGGCAGAGAA TGATGAGACA CTGCAAGCCA AGAAGTCTCG AGTGTCAGAC
    CCTATTAGCA GCTCAGAGAG CTCAGAGGAG GAGGAAGAGG AGGCAGAAAC GGGAACTGCC
    AAAGCCACCC CAAGACCGAC ACCTGTCAAT TCCACAGCTG CAGCTTTGCC ATCAAACATG
    AAACAAAAGG CAAAGACCAA GACAGCCAAC AAGACGGTGA ACTCCGTGCT GCCCCCTGCG
    TCCGGAAAGA CAGCGGTCCA GCTGCTGTCT GGGAAGTCAC CCAAGAAGTC AGCAGAGCCC
    TTGGCCAGCA TTGTCTTGGC CTCAGAAACT GAGGAGGAGG GCAGTGCCCA AGTCCTGGGA
    CCCACTGCCA AGCCTGGAAT GGTGTCAGCA GGCCAAGCCA GCAGTTCCAG CGAGGATACC
    TCCAGCTCAA GCGATGAGAC AGATGTGGAG GTGAAACCCT CAACAAAACC ACAGGCCAAA
    GCTCCAGTAA CTCCTGCCAA GGATCCTCCA GCAAGAACAG CCTCAGGCCC TACCAAGCTA
    GGGGATGTGA CACCCGCATC AGCAAGGGCT GCAGCCACTA AAGCGGCAGA GTCTGAGAGC
    AGTGAGGAGG ACTCAGAGAG TGAGGACGAG GCCCCTGCTG GCCTGCCTAG CCAGGTAACA
    ACCCCTGGAA AAGGTCTTCA TGTCAGAGCT GCCTCAGTTT CTGCCAAGGG GATGTCTAGG
    AAAGGGCCCA TCTCAGCAAC CCCAGAGAAG ACTGGGCCCA TAACCCCCCA AGCCAAGACA
    GGAAGGCCAC AGAAGGATAT GGAAACCAGC AGTGAGGACG ATTCTGACAG TGAGGATGAG
    ATGCCAGTCA CTGTGACCAC TCCTCAGGCA AGGCCTTCCG GGAAGAGCCC TCAGGTCAGA
    GGTACCTCTG CCCCTGCCAA GGAGTCATCC CAGAAAGGGG CTCCTGCAGT CACCCCTGGG
    AAGACAAGAC CTGTGGCAGC CCAGGCCCAG GCAGGGAAGC CAGAGACCAG GAGCAGTGAG
    GAGTCAGAGA GCGACAGTGG AGAGACACCA GCAGCTGGGA CTCTAACTAC AAGTCCTGCC
    AAGGTGAAAC CTTTGGGGAA AAGCCCCCAG GTCAGACCTG CCTCCACTGC AAACCTGGGG
    TCATCTAGAA AAGGAGCCAA CCCACCCTGC CTTGGGAAGG TGGGGTCAGC AGTTCTCAAG
    GTCCAAACGG GAAAGGAAGA AGAGGACTCA GAGAGCAGCA GCGAGGAGTC GGACAGTGAC
    GGGGCTGTGA ACGCAGCCAA GGCAAAGCCC CCAGGGAGAA AAGCCACTCC AGCACTCCCT
    CGGAAGACGG GGCCTGTGGC CACCCAGGTC AAGACTGACA AGGGCAAAGA CCACGCAGAG
    AGCAGCGAGG AGTCGACCGA CAGCGAGGAG GAGGCAGCAC CAGCAGCTTC TGCTGCTCAG
    GCTAAACCAG CTCCGATAAA GCAGATGAAA GCTTCCCCTA GGAAAGGCAC GGCTGCATCC
    ACCACAGGGG CGAGCGCCTC GTCCCCTCGT AAGGCAGGAA CAAAGACCTC TTCAGCCAGC
    CTATCATCCC TGGCTCTGCC CAAGGGCACC CAGAAGCCAG ATGTGGACTC CTCTAGCGAG
    TGGGAGTCAG AAGGAGCTGC TCCTGGCACC ACCGGGGTAC AGGGAAAGTC TGGGGGAAAG
    GGCCTCCAAG GGAGAGCTGC CTCAGGACAA GGGGTGGCCC CACTGCACGC TCAGAAGACA
    GGGCCTTCAG GTGCCCAGGT CAAAGCCACA GCACAGGAAG ACTCAGAGAG CAGCGAAGAG
    GAATCCAGCA GTGAAGAAGA GGATGAGACC CCAGCACAGG TCATGGCCTT GGGGAGACTT
    CCTCCGGCCA AAGCCAACCC ACCTCCCACT AAGACACCTC TGGCATCTGC ATCTGGAAAA
    GCGGCCGCAG TAGTTCCTCC ACCCAAGGGA AAAGCACCTG CGAGTACTGT TCAGAACAGC
    ACCATCTCTG CCAGGGGCCA GCGGGCTGTG CCGGCCACGG GAAAAGCAGG GGCCCCAGCA
    ACCCAAGCAC AGAAGGGGCC CATGGCTGGC ACAGGGGAGG ACTCAGAGAG CAGCAGTGAA
    GAGGAGTCTG ACAGTGAAGA AGAAACTCCA GCCCAGGTCA AGCCTGTGGG GAAGACCTCT
    CAGGTCAGAG CTGCCTCGGC CCCTGTCAAG GAGTCTCCTA ACAAAGGAGC TTATTCAGGA
    ACCTCCAGGA AGACAGGGCC TCCAGCTACC CAAGCTCAGA CAGGGAAGAC AGAGGACTCA
    GAGAGCAGTA GTGAGGAATC TGACAGTGAC AGAGAGATAC CACCAGCCAT CACCCCAGCC
    CAGGATGGTA TCTCCCAGTC TACCAGAAGC AAACTCTCAG GTCTGGCACC CCCAGAGAAG
    AGCACAGAAG AGTCCTCAGA GAGCAGTGAT GAGGATCTGC CATCTGGCCA GGCCATTAAA
    TCCCCTCCAA TTTCTGTCAA CCCTAATCGT GGTCCAGCTG CCCCAGTTCC AACCCCAGAA
    CAACACCAGG CTGTGAACAC AAGGAAGGCC CAGGCCTCAG GCAGCACTGC CCAGAGCTCC
    TCCTCCGAGA GTGAGGATGA GGACATGATT CCTGCCACAC AACCCCCCAC CCTTGCCATC
    AGAACCAATG TGACTACGCC CACAGCCCTC TCACAAACAG CCGCCCAACC CAGCAAGAGT
    GAGCAGTCTA GTCGGATGCC AAAAGGCAAG AAACCAAAGA CAGCGTCCAC TCAGATCAGC
    AGTGCCATGG AAGCACTCCC CGTGACGCTT CCCCAGAGCA CACCTGCCCA GTCCAAAACC
    ACCAACAAGC TGGGGGACCC CAAACTTGCT GAGAAGCAGC AGCTTACCCC AGGCTACCCC
    AAAGCCCCCA GGAGCTCAGA GGACAGCAGT GACACCTCTT CAGAGAGCGA GGAGGATGCC
    AAGAGACCCC AGATGTCCAA GTCGTCCCAA AGGCTGGATC CAGACGCTTC CCAGAAGGAA
    ACTGTGGTAG AGGAGACCCC TACAGAATCC AGCGACGACG AGATGGTGGC CCCCTCACAG
    TCTCTCCTCT CAGGTTATGT GACTCCCGGC TTAACTGTGG CCAATTCCCA GCCTTCAAAA
    GCTACTCCTA GGCCAGACGC CAACCCCTTG GTTTCCTCTG CTCCAGCCAC CAAAGATAAC
    CCAGATGGCA AGCAGAAATC AAAATCCCAA GACTCCACTG CAGACACCAC ACTCCGTAAA
    ACCGGTAGGA AAGAGGCCTC CTCAGGATCC ACACCTCAGA AGCCCAAGAA GCCCAAGAAG
    AGCACCTTGA GCTCACCAGC CCCGGCACAG ACACTGCCGA ACAGCATCAC CCAGCGCCTC
    CTGGAGCAGC CCTGGCCCCT GAGTGAGGCA CAGGTGCAGG CCTCTGTGAT GAAGGTCCTG
    ACAGAACTGC TGGAGCAGGA AAGGCAGAAG GCCACCGAGG CCATCAAGGA GAGTGGAAAG
    AAGGGCCAGA AACGGAAACT ATCAGGGGAC CAGGTAGAAG CTGGGGCCCC AAAGAACAAG
    AAGAAGAAGC AGCAGCTTGC GGCTGGGACA AGTGCTGGTT CCCCAGAAAA GGCCTCCAGG
    ACTTCCAAGG CAAAATCAAA ACTAAACAAA GGGAGTGCTG GTGGCAAGGG GAAGGGGTCT
    CCTGTCCCCC AAGGAGCCAA GGAGAAGCCC GAAGGCAAGC TGGGGATAAA GCTTGAGAGT
    GGAGAGCAGA GTGACCCGAA GAGCAAGAAG GAAAAGAAGA AATCCAGTAA GAAAAGAAAA
    AAGGAAAAAA GACCCCAGCC AAGGACTCTG CCTCACCGGT CCAGAAGAAG AAAAAGAAGA
    AGAAGAAGAC AGCCGAGCCT GCTGTGTGAG GGCCAGGCAG CGCATAGAAC AGAGTCTTGG
    CAGAGCGTGA CCATCCCCAG GCCTCTGACC TCTGGGCACT GTGAGTTGGG ACTGGACTTT
    TTAATTTGCC CTCCCTCGGC AGAAGGTGAT GGCTGGCGTT GCTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001099613.1
    CDS127..4314
    Translation

    Target ORF information:

    RefSeq Version NM_001106143.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus treacle ribosome biogenesis factor 1 (Tcof1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001106143.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCCGAGG CCAGGAAGCG GCGGGAGCTG CTTCCCCTTA TTTACCATCA TCTGTTGCAA 
    GCAGGCTACG TCCGCGCGGC GCGGGAAGTT AAGGAGCAGA GCGGCCAGAA GAATTTCCTG
    ACTCATCCCG TCACCCTTCT GGACATCTAT ACACACTGGC AACAGACCTC AGAGCTCGGC
    CTGAAGCAGA AGGCAGAGAA TGATGAGACA CTGCAAGCCA AGAAGTCTCG AGTGTCAGAC
    CCTATTAGCA GCTCAGAGAG CTCAGAGGAG GAGGAAGAGG AGGCAGAAAC GGGAACTGCC
    AAAGCCACCC CAAGACCGAC ACCTGTCAAT TCCACAGCTG CAGCTTTGCC ATCAAACATG
    AAACAAAAGG CAAAGACCAA GACAGCCAAC AAGACGGTGA ACTCCGTGCT GCCCCCTGCG
    TCCGGAAAGA CAGCGGTCCA GCTGCTGTCT GGGAAGTCAC CCAAGAAGTC AGCAGAGCCC
    TTGGCCAGCA TTGTCTTGGC CTCAGAAACT GAGGAGGAGG GCAGTGCCCA AGTCCTGGGA
    CCCACTGCCA AGCCTGGAAT GGTGTCAGCA GGCCAAGCCA GCAGTTCCAG CGAGGATACC
    TCCAGCTCAA GCGATGAGAC AGATGTGGAG GTGAAACCCT CAACAAAACC ACAGGCCAAA
    GCTCCAGTAA CTCCTGCCAA GGATCCTCCA GCAAGAACAG CCTCAGGCCC TACCAAGCTA
    GGGGATGTGA CACCCGCATC AGCAAGGGCT GCAGCCACTA AAGCGGCAGA GTCTGAGAGC
    AGTGAGGAGG ACTCAGAGAG TGAGGACGAG GCCCCTGCTG GCCTGCCTAG CCAGGTAACA
    ACCCCTGGAA AAGGTCTTCA TGTCAGAGCT GCCTCAGTTT CTGCCAAGGG GATGTCTAGG
    AAAGGGCCCA TCTCAGCAAC CCCAGAGAAG ACTGGGCCCA TAACCCCCCA AGCCAAGACA
    GGAAGGCCAC AGAAGGATAT GGAAACCAGC AGTGAGGACG ATTCTGACAG TGAGGATGAG
    ATGCCAGTCA CTGTGACCAC TCCTCAGGCA AGGCCTTCCG GGAAGAGCCC TCAGGTCAGA
    GGTACCTCTG CCCCTGCCAA GGAGTCATCC CAGAAAGGGG CTCCTGCAGT CACCCCTGGG
    AAGACAAGAC CTGTGGCAGC CCAGGCCCAG GCAGGGAAGC CAGAGACCAG GAGCAGTGAG
    GAGTCAGAGA GCGACAGTGG AGAGACACCA GCAGCTGGGA CTCTAACTAC AAGTCCTGCC
    AAGGTGAAAC CTTTGGGGAA AAGCCCCCAG GTCAGACCTG CCTCCACTGC AAACCTGGGG
    TCATCTAGAA AAGGAGCCAA CCCACCCTGC CTTGGGAAGG TGGGGTCAGC AGTTCTCAAG
    GTCCAAACGG GAAAGGAAGA AGAGGACTCA GAGAGCAGCA GCGAGGAGTC GGACAGTGAC
    GGGGCTGTGA ACGCAGCCAA GGCAAAGCCC CCAGGGAGAA AAGCCACTCC AGCACTCCCT
    CGGAAGACGG GGCCTGTGGC CACCCAGGTC AAGACTGACA AGGGCAAAGA CCACGCAGAG
    AGCAGCGAGG AGTCGACCGA CAGCGAGGAG GAGGCAGCAC CAGCAGCTTC TGCTGCTCAG
    GCTAAACCAG CTCCGATAAA GCAGATGAAA GCTTCCCCTA GGAAAGGCAC GGCTGCATCC
    ACCACAGGGG CGAGCGCCTC GTCCCCTCGT AAGGCAGGAA CAAAGACCTC TTCAGCCAGC
    CTATCATCCC TGGCTCTGCC CAAGGGCACC CAGAAGCCAG ATGTGGACTC CTCTAGCGAG
    TGGGAGTCAG AAGGAGCTGC TCCTGGCACC ACCGGGGTAC AGGGAAAGTC TGGGGGAAAG
    GGCCTCCAAG GGAGAGCTGC CTCAGGACAA GGGGTGGCCC CACTGCACGC TCAGAAGACA
    GGGCCTTCAG GTGCCCAGGT CAAAGCCACA GCACAGGAAG ACTCAGAGAG CAGCGAAGAG
    GAATCCAGCA GTGAAGAAGA GGATGAGACC CCAGCACAGG TCATGGCCTT GGGGAGACTT
    CCTCCGGCCA AAGCCAACCC ACCTCCCACT AAGACACCTC TGGCATCTGC ATCTGGAAAA
    GCGGCCGCAG TAGTTCCTCC ACCCAAGGGA AAAGCACCTG CGAGTACTGT TCAGAACAGC
    ACCATCTCTG CCAGGGGCCA GCGGGCTGTG CCGGCCACGG GAAAAGCAGG GGCCCCAGCA
    ACCCAAGCAC AGAAGGGGCC CATGGCTGGC ACAGGGGAGG ACTCAGAGAG CAGCAGTGAA
    GAGGAGTCTG ACAGTGAAGA AGAAACTCCA GCCCAGGTCA AGCCTGTGGG GAAGACCTCT
    CAGGTCAGAG CTGCCTCGGC CCCTGTCAAG GAGTCTCCTA ACAAAGGAGC TTATTCAGGA
    ACCTCCAGGA AGACAGGGCC TCCAGCTACC CAAGCTCAGA CAGGGAAGAC AGAGGACTCA
    GAGAGCAGTA GTGAGGAATC TGACAGTGAC AGAGAGATAC CACCAGCCAT CACCCCAGCC
    CAGGATGGTA TCTCCCAGTC TACCAGAAGC AAACTCTCAG GTCTGGCACC CCCAGAGAAG
    AGCACAGAAG AGTCCTCAGA GAGCAGTGAT GAGGATCTGC CATCTGGCCA GGCCATTAAA
    TCCCCTCCAA TTTCTGTCAA CCCTAATCGT GGTCCAGCTG CCCCAGTTCC AACCCCAGAA
    CAACACCAGG CTGTGAACAC AAGGAAGGCC CAGGCCTCAG GCAGCACTGC CCAGAGCTCC
    TCCTCCGAGA GTGAGGATGA GGACATGATT CCTGCCACAC AACCCCCCAC CCTTGCCATC
    AGAACCAATG TGACTACGCC CACAGCCCTC TCACAAACAG CCGCCCAACC CAGCAAGAGT
    GAGCAGTCTA GTCGGATGCC AAAAGGCAAG AAACCAAAGA CAGCGTCCAC TCAGATCAGC
    AGTGCCATGG AAGCACTCCC CGTGACGCTT CCCCAGAGCA CACCTGCCCA GTCCAAAACC
    ACCAACAAGC TGGGGGACCC CAAACTTGCT GAGAAGCAGC AGCTTACCCC AGGCTACCCC
    AAAGCCCCCA GGAGCTCAGA GGACAGCAGT GACACCTCTT CAGAGAGCGA GGAGGATGCC
    AAGAGACCCC AGATGTCCAA GTCGTCCCAA AGGCTGGATC CAGACGCTTC CCAGAAGGAA
    ACTGTGGTAG AGGAGACCCC TACAGAATCC AGCGACGACG AGATGGTGGC CCCCTCACAG
    TCTCTCCTCT CAGGTTATGT GACTCCCGGC TTAACTGTGG CCAATTCCCA GCCTTCAAAA
    GCTACTCCTA GGCCAGACGC CAACCCCTTG GTTTCCTCTG CTCCAGCCAC CAAAGATAAC
    CCAGATGGCA AGCAGAAATC AAAATCCCAA GACTCCACTG CAGACACCAC ACTCCGTAAA
    ACCGGTAGGA AAGAGGCCTC CTCAGGATCC ACACCTCAGA AGCCCAAGAA GCCCAAGAAG
    AGCACCTTGA GCTCACCAGC CCCGGCACAG ACACTGCCGA ACAGCATCAC CCAGCGCCTC
    CTGGAGCAGC CCTGGCCCCT GAGTGAGGCA CAGGTGCAGG CCTCTGTGAT GAAGGTCCTG
    ACAGAACTGC TGGAGCAGGA AAGGCAGAAG GCCACCGAGG CCATCAAGGA GAGTGGAAAG
    AAGGGCCAGA AACGGAAACT ATCAGGGGAC CAGGTAGAAG CTGGGGCCCC AAAGAACAAG
    AAGAAGAAGC AGCAGCTTGC GGCTGGGACA AGTGCTGGTT CCCCAGAAAA GGCCTCCAGG
    ACTTCCAAGG CAAAATCAAA ACTAAACAAA GGGAGTGCTG GTGGCAAGGG GAAGGGGTCT
    CCTGTCCCCC AAGGAGCCAA GGAGAAGCCC GAAGGCAAGC TGGGGATAAA GCTTGAGAGT
    GGAGAGCAGA GTGACCCGAA GAGCAAGAAG GAAAAGAAGA AATCCAGTAA GAAAAGAAAA
    AAGGAAAAAA GACCCCAGCC AAGGACTCTG CCTCACCGGT CCAGAAGAAG AAAAAGAAGA
    AGAAGAAGAC AGCCGAGCCT GCTGTGTGAG GGCCAGGCAG CGCATAGAAC AGAGTCTTGG
    CAGAGCGTGA CCATCCCCAG GCCTCTGACC TCTGGGCACT GTGAGTTGGG ACTGGACTTT
    TTAATTTGCC CTCCCTCGGC AGAAGGTGAT GGCTGGCGTT GCTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa44960
    Clone ID Related Accession (Same CDS sequence) XM_017600890.1
    Accession Version XM_017600890.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4074bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product treacle protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005117.4) and transcript sequence (FQ232316.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 5 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCCGAGG CCAGGAAGCG GCGGGAGCTG CTTCCCCTTA TTTACCATCA TCTGTTGCAA 
    GCAGGCTACG TCCGCGCGGC GCGGGAAGTT AAGGAGCAGA GCGGCCAGAA GAATTTCCTG
    ACTCATCCCG TCACCCTTCT GGACATCTAT ACACACTGGC AACAGACCTC AGAGCTCGGC
    CTGAAGCAGA AGGCAGAGAA TGATGAGACA CTGCAAGCCA AGAAGTCTCG AGTGTCAGAC
    CCTATTAGCA GCTCAGAGAG CTCAGAGGAG GAGGAAGAGG AGGCAGAAAC GGGAACTGCC
    AAAGCCACCC CAAGACCGAC ACCTGTCAAT TCCACAGCTG CAGCTTTGCC ATCAAACATG
    AAACAAAAGG CAAAGACCAA GACAGCCAAC AAGACGGTGA ACTCCGTGCT GCCCCCTGCG
    TCCGGAAAGA CAGCGGTCCA GCTGCTGTCT GGGAAGTCAC CCAAGAAGTC AGCAGAGCCC
    TTGGCCAGCA TTGTCTTGGC CTCAGAAACT GAGGAGGAGG GCAGTGCCCA AGTCCTGGGA
    CCCACTGCCA AGCCTGGAAT GGTGTCAGCA GGCCAAGCCA GCAGTTCCAG CGAGGATACC
    TCCAGCTCAA GCGATGAGAC AGATGTGGAG GTGAAACCCT CAACAAAACC ACAGGCCAAA
    GCTCCAGTAA CTCCTGCCAA GGATCCTCCA GCAAGAACAG CCTCAGGCCC TACCAAGCTA
    GGGGATGTGA CACCCGCATC AGCAAGGGCT GCAGCCACTA AAGCGGCAGA GTCTGAGAGC
    AGTGAGGAGG ACTCAGAGAG TGAGGACGAG GCCCCTGCTG GCCTGCCTAG CCAGGTAACA
    ACCCCTGGAA AAGGTCTTCA TGTCAGAGCT GCCTCAGTTT CTGCCAAGGG GATGTCTAGG
    AAAGGGCCCA TCTCAGCAAC CCCAGAGAAG ACTGGGCCCA TAACCCCCCA AGCCAAGACA
    GGAAGGCCAC AGAAGGATAT GGAAACCAGC AGTGAGGACG ATTCTGACAG TGAGGATGAG
    ATGCCAGTCA CTGTGACCAC TCCTCAGGCA AGGCCTTCCG GGAAGAGCCC TCAGGTCAGA
    GGTACCTCTG CCCCTGCCAA GGAGTCATCC CAGAAAGGGG CTCCTGCAGT CACCCCTGGG
    AAGACAAGAC CTGTGGCAGC CCAGGCCCAG GCAGGGAAGC CAGAGACCAG GAGCAGTGAG
    GAGTCAGAGA GCGACAGTGG AGAGACACCA GCAGCTGGGA CTCTAACTAC AAGTCCTGCC
    AAGGTGAAAC CTTTGGGGAA AAGCCCCCAG GTCAGACCTG CCTCCACTGC AAACCTGGGG
    TCATCTAGAA AAGGAGCCAA CCCACCCTGC CTTGGGAAGG TGGGGTCAGC AGTTCTCAAG
    GTCCAAACGG GAAAGGAAGA AGAGGACTCA GAGAGCAGCA GCGAGGAGTC GGACAGTGAC
    GGGGCTGTGA ACGCAGCCAA GGCAAAGCCC CCAGGGAGAA AAGCCACTCC AGCACTCCCT
    CGGAAGACGG GGCCTGTGGC CACCCAGGTC AAGACTGACA AGGGCAAAGA CCACGCAGAG
    AGCAGCGAGG AGTCGACCGA CAGCGAGGAG GAGGCAGCAC CAGCAGCTTC TGCTGCTCAG
    GCTAAACCAG CTCCGATAAA GCAGATGAAA GCTTCCCCTA GGAAAGGCAC GGCTGCATCC
    ACCACAGGGG CGAGCGCCTC GTCCCCTCGT AAGGCAGGAA CAAAGACCTC TTCAGCCAGC
    CTATCATCCC TGGCTCTGCC CAAGGGCACC CAGAAGCCAG ATGTGGACTC CTCTAGCGAG
    TGGGAGTCAG AAGGAGCTGC TCCTGGCACC ACCGGGGTAC AGGGAAAGTC TGGGGGAAAG
    GGCCTCCAAG GGAGAGCTGC CTCAGGACAA GGGGTGGCCC CACTGCACGC TCAGAAGACA
    GGGCCTTCAG GTGCCCAGGT CAAAGCCACA GCACAGGAAG ACTCAGAGAG CAGCGAAGAG
    GAATCCAGCA GTGAAGAAGA GGATGAGACC CCAGCACAGG TCATGGCCTT GGGGAGACTT
    CCTCCGGCCA AAGCCAACCC ACCTCCCACT AAGACACCTC TGGCATCTGC ATCTGGAAAA
    GCGGCCGCAG TAGTTCCTCC ACCCAAGGGA AAAGCACCTG CGAGTACTGT TCAGAACAGC
    ACCATCTCTG CCAGGGGCCA GCGGGCTGTG CCGGCCACGG GAAAAGCAGG GGCCCCAGCA
    ACCCAAGCAC AGAAGGGGCC CATGGCTGGC ACAGGGGAGG ACTCAGAGAG CAGCAGTGAA
    GAGGAGTCTG ACAGTGAAGA AGAAACTCCA GCCCAGGTCA AGCCTGTGGG GAAGACCTCT
    CAGGTCAGAG CTGCCTCGGC CCCTGTCAAG GAGTCTCCTA ACAAAGGAGC TTATTCAGGA
    ACCTCCAGGA AGACAGGGCC TCCAGCTACC CAAGCTCAGA CAGGGAAGAC AGAGGACTCA
    GAGAGCAGTA GTGAGGAATC TGACAGTGAC AGAGAGATAC CACCAGCCAT CACCCCAGCC
    CAGGATGGTA TCTCCCAGTC TACCAGAAGC AAACTCTCAG GTCTGGCACC CCCAGAGAAG
    AGCACAGAAG AGTCCTCAGA GAGCAGTGAT GAGGATCTGC CATCTGGCCA GGCCATTAAA
    TCCCCTCCAA TTTCTGTCAA CCCTAATCGT GGTCCAGCTG CCCCAGTTCC AACCCCAGAA
    CAACACCAGG CTGTGAACAC AAGGAAGGCC CAGGCCTCAG GCAGCACTGC CCAGAGCTCC
    TCCTCCGAGA GTGAGGATGA GGACATGATT CCTGCCACAC AACCCCCCAC CCTTGCCATC
    AGAACCAATG TGACTACGCC CACAGCCCTC TCACAAACAG CCGCCCAACC CAGCAAGAGT
    GAGCAGTCTA GTCGGATGCC AAAAGGCAAG AAACCAAAGA CAGCGTCCAC TCAGATCAGC
    AGTGCCATGG AAGCACTCCC CGTGACGCTT CCCCAGAGCA CACCTGCCCA GTCCAAAACC
    ACCAACAAGC TGGGGGACCC CAAACTTGCT GAGAAGCAGC AGCTTACCCC AGGCTACCCC
    AAAGCCCCCA GGAGCTCAGA GGACAGCAGT GACACCTCTT CAGAGAGCGA GGAGGATGCC
    AAGAGACCCC AGATGTCCAA GTCGTCCCAA AGGCTGGATC CAGACGCTTC CCAGAAGGAA
    ACTGTGGTAG AGGAGACCCC TACAGAATCC AGCGACGACG AGATGGTGGC CCCCTCACAG
    TCTCTCCTCT CAGGTTATGT GACTCCCGGC TTAACTGTGG CCAATTCCCA GCCTTCAAAA
    GCTACTCCTA GGCCAGACGC CAACCCCTTG GTTTCCTCTG CTCCAGCCAC CAAAGATAAC
    CCAGATGGCA AGCAGAAATC AAAATCCCAA GACTCCACTG CAGACACCAC ACTCCGTAAA
    ACCGGTAGGA AAGAGGCCTC CTCAGGATCC ACACCTCAGA AGCCCAAGAA GCCCAAGAAG
    AGCACCTTGA GCTCACCAGC CCCGGCACAG ACACTGCCGA ACAGCATCAC CCAGCGCCTC
    CTGGAGCAGC CCTGGCCCCT GAGTGAGGCA CAGGTGCAGG CCTCTGTGAT GAAGGTCCTG
    ACAGAACTGC TGGAGCAGGA AAGGCAGAAG GCCACCGAGG CCATCAAGGA GAGTGGAAAG
    AAGGGCCAGA AACGGAAACT ATCAGGGGAC CAGGTAGAAG CTGGGGCCCC AAAGAACAAG
    AAGAAGAAGC AGCAGCTTGC GGCTGGGACA AGTGCTGGTT CCCCAGAAAA GGCCTCCAGG
    ACTTCCAAGG CAAAATCAAA ACTAAACAAA GGGAGTGCTG GTGGCAAGGG GAAGGGGTCT
    CCTGTCCCCC AAGGAGCCAA GGAGAAGCCC GAAGGCAAGC TGGGGATAAA GCTTGAGAGT
    GGAGAGCAGA GTGACCCGAA GAGCAAGAAG GAAAAGAAGA AATCCAGTAA GAAAAAAAAA
    GACAAGGAAA AAAAGGAAAA GAAAAAAGGA AAAAAGACCC CAGCCAAGGA CTCTGCCTCA
    CCGGTCCAGA AGAAGAAAAA GAAGAAGAAG AAGACAGCCG AGCCTGCTGT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017456379.1
    CDS110..4183
    Translation

    Target ORF information:

    RefSeq Version XM_017600890.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus treacle ribosome biogenesis factor 1 (Tcof1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017600890.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCCGAGG CCAGGAAGCG GCGGGAGCTG CTTCCCCTTA TTTACCATCA TCTGTTGCAA 
    GCAGGCTACG TCCGCGCGGC GCGGGAAGTT AAGGAGCAGA GCGGCCAGAA GAATTTCCTG
    ACTCATCCCG TCACCCTTCT GGACATCTAT ACACACTGGC AACAGACCTC AGAGCTCGGC
    CTGAAGCAGA AGGCAGAGAA TGATGAGACA CTGCAAGCCA AGAAGTCTCG AGTGTCAGAC
    CCTATTAGCA GCTCAGAGAG CTCAGAGGAG GAGGAAGAGG AGGCAGAAAC GGGAACTGCC
    AAAGCCACCC CAAGACCGAC ACCTGTCAAT TCCACAGCTG CAGCTTTGCC ATCAAACATG
    AAACAAAAGG CAAAGACCAA GACAGCCAAC AAGACGGTGA ACTCCGTGCT GCCCCCTGCG
    TCCGGAAAGA CAGCGGTCCA GCTGCTGTCT GGGAAGTCAC CCAAGAAGTC AGCAGAGCCC
    TTGGCCAGCA TTGTCTTGGC CTCAGAAACT GAGGAGGAGG GCAGTGCCCA AGTCCTGGGA
    CCCACTGCCA AGCCTGGAAT GGTGTCAGCA GGCCAAGCCA GCAGTTCCAG CGAGGATACC
    TCCAGCTCAA GCGATGAGAC AGATGTGGAG GTGAAACCCT CAACAAAACC ACAGGCCAAA
    GCTCCAGTAA CTCCTGCCAA GGATCCTCCA GCAAGAACAG CCTCAGGCCC TACCAAGCTA
    GGGGATGTGA CACCCGCATC AGCAAGGGCT GCAGCCACTA AAGCGGCAGA GTCTGAGAGC
    AGTGAGGAGG ACTCAGAGAG TGAGGACGAG GCCCCTGCTG GCCTGCCTAG CCAGGTAACA
    ACCCCTGGAA AAGGTCTTCA TGTCAGAGCT GCCTCAGTTT CTGCCAAGGG GATGTCTAGG
    AAAGGGCCCA TCTCAGCAAC CCCAGAGAAG ACTGGGCCCA TAACCCCCCA AGCCAAGACA
    GGAAGGCCAC AGAAGGATAT GGAAACCAGC AGTGAGGACG ATTCTGACAG TGAGGATGAG
    ATGCCAGTCA CTGTGACCAC TCCTCAGGCA AGGCCTTCCG GGAAGAGCCC TCAGGTCAGA
    GGTACCTCTG CCCCTGCCAA GGAGTCATCC CAGAAAGGGG CTCCTGCAGT CACCCCTGGG
    AAGACAAGAC CTGTGGCAGC CCAGGCCCAG GCAGGGAAGC CAGAGACCAG GAGCAGTGAG
    GAGTCAGAGA GCGACAGTGG AGAGACACCA GCAGCTGGGA CTCTAACTAC AAGTCCTGCC
    AAGGTGAAAC CTTTGGGGAA AAGCCCCCAG GTCAGACCTG CCTCCACTGC AAACCTGGGG
    TCATCTAGAA AAGGAGCCAA CCCACCCTGC CTTGGGAAGG TGGGGTCAGC AGTTCTCAAG
    GTCCAAACGG GAAAGGAAGA AGAGGACTCA GAGAGCAGCA GCGAGGAGTC GGACAGTGAC
    GGGGCTGTGA ACGCAGCCAA GGCAAAGCCC CCAGGGAGAA AAGCCACTCC AGCACTCCCT
    CGGAAGACGG GGCCTGTGGC CACCCAGGTC AAGACTGACA AGGGCAAAGA CCACGCAGAG
    AGCAGCGAGG AGTCGACCGA CAGCGAGGAG GAGGCAGCAC CAGCAGCTTC TGCTGCTCAG
    GCTAAACCAG CTCCGATAAA GCAGATGAAA GCTTCCCCTA GGAAAGGCAC GGCTGCATCC
    ACCACAGGGG CGAGCGCCTC GTCCCCTCGT AAGGCAGGAA CAAAGACCTC TTCAGCCAGC
    CTATCATCCC TGGCTCTGCC CAAGGGCACC CAGAAGCCAG ATGTGGACTC CTCTAGCGAG
    TGGGAGTCAG AAGGAGCTGC TCCTGGCACC ACCGGGGTAC AGGGAAAGTC TGGGGGAAAG
    GGCCTCCAAG GGAGAGCTGC CTCAGGACAA GGGGTGGCCC CACTGCACGC TCAGAAGACA
    GGGCCTTCAG GTGCCCAGGT CAAAGCCACA GCACAGGAAG ACTCAGAGAG CAGCGAAGAG
    GAATCCAGCA GTGAAGAAGA GGATGAGACC CCAGCACAGG TCATGGCCTT GGGGAGACTT
    CCTCCGGCCA AAGCCAACCC ACCTCCCACT AAGACACCTC TGGCATCTGC ATCTGGAAAA
    GCGGCCGCAG TAGTTCCTCC ACCCAAGGGA AAAGCACCTG CGAGTACTGT TCAGAACAGC
    ACCATCTCTG CCAGGGGCCA GCGGGCTGTG CCGGCCACGG GAAAAGCAGG GGCCCCAGCA
    ACCCAAGCAC AGAAGGGGCC CATGGCTGGC ACAGGGGAGG ACTCAGAGAG CAGCAGTGAA
    GAGGAGTCTG ACAGTGAAGA AGAAACTCCA GCCCAGGTCA AGCCTGTGGG GAAGACCTCT
    CAGGTCAGAG CTGCCTCGGC CCCTGTCAAG GAGTCTCCTA ACAAAGGAGC TTATTCAGGA
    ACCTCCAGGA AGACAGGGCC TCCAGCTACC CAAGCTCAGA CAGGGAAGAC AGAGGACTCA
    GAGAGCAGTA GTGAGGAATC TGACAGTGAC AGAGAGATAC CACCAGCCAT CACCCCAGCC
    CAGGATGGTA TCTCCCAGTC TACCAGAAGC AAACTCTCAG GTCTGGCACC CCCAGAGAAG
    AGCACAGAAG AGTCCTCAGA GAGCAGTGAT GAGGATCTGC CATCTGGCCA GGCCATTAAA
    TCCCCTCCAA TTTCTGTCAA CCCTAATCGT GGTCCAGCTG CCCCAGTTCC AACCCCAGAA
    CAACACCAGG CTGTGAACAC AAGGAAGGCC CAGGCCTCAG GCAGCACTGC CCAGAGCTCC
    TCCTCCGAGA GTGAGGATGA GGACATGATT CCTGCCACAC AACCCCCCAC CCTTGCCATC
    AGAACCAATG TGACTACGCC CACAGCCCTC TCACAAACAG CCGCCCAACC CAGCAAGAGT
    GAGCAGTCTA GTCGGATGCC AAAAGGCAAG AAACCAAAGA CAGCGTCCAC TCAGATCAGC
    AGTGCCATGG AAGCACTCCC CGTGACGCTT CCCCAGAGCA CACCTGCCCA GTCCAAAACC
    ACCAACAAGC TGGGGGACCC CAAACTTGCT GAGAAGCAGC AGCTTACCCC AGGCTACCCC
    AAAGCCCCCA GGAGCTCAGA GGACAGCAGT GACACCTCTT CAGAGAGCGA GGAGGATGCC
    AAGAGACCCC AGATGTCCAA GTCGTCCCAA AGGCTGGATC CAGACGCTTC CCAGAAGGAA
    ACTGTGGTAG AGGAGACCCC TACAGAATCC AGCGACGACG AGATGGTGGC CCCCTCACAG
    TCTCTCCTCT CAGGTTATGT GACTCCCGGC TTAACTGTGG CCAATTCCCA GCCTTCAAAA
    GCTACTCCTA GGCCAGACGC CAACCCCTTG GTTTCCTCTG CTCCAGCCAC CAAAGATAAC
    CCAGATGGCA AGCAGAAATC AAAATCCCAA GACTCCACTG CAGACACCAC ACTCCGTAAA
    ACCGGTAGGA AAGAGGCCTC CTCAGGATCC ACACCTCAGA AGCCCAAGAA GCCCAAGAAG
    AGCACCTTGA GCTCACCAGC CCCGGCACAG ACACTGCCGA ACAGCATCAC CCAGCGCCTC
    CTGGAGCAGC CCTGGCCCCT GAGTGAGGCA CAGGTGCAGG CCTCTGTGAT GAAGGTCCTG
    ACAGAACTGC TGGAGCAGGA AAGGCAGAAG GCCACCGAGG CCATCAAGGA GAGTGGAAAG
    AAGGGCCAGA AACGGAAACT ATCAGGGGAC CAGGTAGAAG CTGGGGCCCC AAAGAACAAG
    AAGAAGAAGC AGCAGCTTGC GGCTGGGACA AGTGCTGGTT CCCCAGAAAA GGCCTCCAGG
    ACTTCCAAGG CAAAATCAAA ACTAAACAAA GGGAGTGCTG GTGGCAAGGG GAAGGGGTCT
    CCTGTCCCCC AAGGAGCCAA GGAGAAGCCC GAAGGCAAGC TGGGGATAAA GCTTGAGAGT
    GGAGAGCAGA GTGACCCGAA GAGCAAGAAG GAAAAGAAGA AATCCAGTAA GAAAAAAAAA
    GACAAGGAAA AAAAGGAAAA GAAAAAAGGA AAAAAGACCC CAGCCAAGGA CTCTGCCTCA
    CCGGTCCAGA AGAAGAAAAA GAAGAAGAAG AAGACAGCCG AGCCTGCTGT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.