Tcof1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tcof1
Entrez Gene ID	21453
Full Name	treacle ribosome biogenesis factor 1
Synonyms	AA408847,AW209012,treacle
General protein information	Preferred Names treacle ribosome biogenesis factor 1 Names treacle protein Treacher Collins Franceschetti syndrome 1, homolog treacher Collins syndrome protein homolog
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 18 Map Location: 18 E1\|18 34.41 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001198984.1	NP_001185913.1	treacle protein isoform 1
XM_011246874.2	XP_011245176.1	treacle protein isoform X2
NM_011552.3	NP_035682.1	treacle protein isoform 2
XM_006525777.2	XP_006525840.1	treacle protein isoform X1
XM_011246875.2	XP_011245177.1	treacle protein isoform X3

Pathways from BioSystems

Homologies

Homo sapiens (human)	TCOF1	NP_001128717.1
Bos taurus (cattle)	TCOF1	XP_002689339.2
Rattus norvegicus (Norway rat)	Tcof1	NP_001099613.1
Canis lupus familiaris (dog)	TCOF1	NP_001003057.1
Pan troglodytes (chimpanzee)	TCOF1	XP_527074.4
Mus musculus (house mouse)	Tcof1	NP_001185913.1
	TCOF1	NP_001035041.1

Gene Ontology
Bibliography

Related articles in PubMed

Face off against ROS: Tcof1/Treacle safeguards neuroepithelial cells and progenitor neural crest cells from oxidative stress during craniofacial development.
Sakai D, Trainor PA
Development, growth & differentiation58(7)577-85(2016 Sep)

A quantitative method for defining high-arched palate using the Tcof1(+/-) mutant mouse as a model.
Conley ZR, Hague M, Kurosaka H, Dixon J, Dixon MJ, Trainor PA
Developmental biology415(2)296-305(2016 07)

Prevention of Treacher Collins syndrome craniofacial anomalies in mouse models via maternal antioxidant supplementation.
Sakai D, Dixon J, Achilleos A, Dixon M, Trainor PA
Nature communications710328(2016 Jan)

Quantitative trait loci affecting the 3D skull shape and size in mouse and prioritization of candidate genes in-silico.
Maga AM, Navarro N, Cunningham ML, Cox TC
Frontiers in physiology692(2015)

Resistance to noise-induced hearing loss in 129S6 and MOLF mice: identification of independent, overlapping, and interacting chromosomal regions.
Street VA, Kujawa SG, Manichaikul A, Broman KW, Kallman JC, Shilling DJ, Iwata AJ, Robinson LC, Robbins CA, Li J, Liberman MC, Tempel BL
Journal of the Association for Research in Otolaryngology : JARO15(5)721-38(2014 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Role of Tcof1 in normal embryonic development, the correlation between genetic and environmental factors on the severity of craniofacial abnormalities, and the prospect for prenatal prevention of craniofacial anomalies. [review]
Title: Face off against ROS: Tcof1/Treacle safeguards neuroepithelial cells and progenitor neural crest cells from oxidative stress during craniofacial development.

Tcof1 acts as a modifier of Pax3 during enteric nervous system development.
Title: Tcof1 acts as a modifier of Pax3 during enteric nervous system development and in the pathogenesis of colonic aganglionosis.

identified Tcof1 as an important regulator of vagal neural crest cells (NCC) development and enteric nervous system formation; Tcof1 loss-of-function results in a deficiency of vagal NCC and their delayed colonization of the gut during early embryogenesis, which mimics the early stages of Hirschsprung's disease
Title: Balancing neural crest cell intrinsic processes with those of the microenvironment in Tcof1 haploinsufficient mice enables complete enteric nervous system formation.

our research has therefore identified Treacle and as novel in vivo regulators of spindle fidelity, mitotic progression, and proliferation in the maintenance and localization of neural progenitor cells.
Title: Mammalian neurogenesis requires Treacle-Plk1 for precise control of spindle orientation, mitotic progression, and maintenance of neural progenitor cells.

Loss-of-function mutation in Tcof1 results in defects in middle ear postnatal development and conductive hearing loss.
Title: Defects in middle ear cavitation cause conductive hearing loss in the Tcof1 mutant mouse.

The following Tcof1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tcof1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu15909	NM_001198984.1 Latest version!	Mus musculus treacle ribosome biogenesis factor 1 (Tcof1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OMu16275	NM_011552.3 Latest version!	Mus musculus treacle ribosome biogenesis factor 1 (Tcof1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OMu54472	XM_006525777.2 Latest version!	Mus musculus treacle ribosome biogenesis factor 1 (Tcof1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OMu68855	XM_011246874.2 Latest version!	Mus musculus treacle ribosome biogenesis factor 1 (Tcof1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OMu68856	XM_011246875.2 Latest version!	Mus musculus treacle ribosome biogenesis factor 1 (Tcof1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tcof1 ( NM_001198984.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001185913.1 Mus musculus treacle ribosome biogenesis factor 1 (Tcof1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu15909
Clone ID Related Accession (Same CDS sequence)	NM_001198984.1
Accession Version	NM_001198984.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4071bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	treacle protein isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC139759.4 and BC057342.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC057342.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTG CTTCCCCTCA TTTACCATCA TCTGTTGCAA 
GCAGGCTACG TCCGCGCGGC GCGGGAAGTA AAGGAGCAGA GCGGCCAGAA GAGTTTCCTG 
ACTCAGCCCG TCACCCTTCT GGACATCTAT ACACACTGGC AACAGACCTC AGAGCTTGGC 
CAGAAGCAGA AGGCAGAGGA TGATGAGACC CTTCAGGCTA AGAAGTCTCG AGTGTCGGAT 
CCTGTTAGCA GCTCAGAGAG CTCGGATCAG GAGAAGGAAG AGGAGGCAGC AACCGAAAGG 
GCCAAAGCCA CCCCAAGACC GACACCTGTC AATTCTGCAA CCGCAGCTTT GCCATCAAAA 
GTAAAAGAAA AGGGAAAGAC CAAGACAGCC AACAAGACGG TGAACTCTGT GTCGCACCCT 
GGGTCCGGAA AGACGGTGGT CCACCTGCTC TCTGGGAAGT CACCCAAAAA GTCAGCAGAG 
CCCTTGGCAA ACACTGTCTT GGCCTCAGAA ACTGAGGAGG AGGGCAATGC CCAAGCCCTC 
GGACCCACTG CCAAGTCTGG AACGGTGTCA GCGGGCCAAG GCAGCAGTTC CAGTGAAGAT 
TCCTCCATCT CAAGCGATGA GACAGATGTC GAGGTGAAAT CTCCAGCAAA ACCAGCCCAG 
GCCAAAGCTT CAGCAGCCCC TGCCAAGGAT CCTCCAGCAA GAACAGCCCC AGGCCCTACC 
AAGTTAGGGA ATGTGGCGCC CACACCTGCT AAACCAGCCA GGGCGGCAGC GGCAGCTGCT 
GCTGCTGCTG TGGCTGCTGC TGCTGCTGCA GCAGCAGAAG AGTCTGAGAG CAGTGAGGAG 
GACTCAGACA GTGAGGACGA GGCCCCTGCT GGTCTGCCCA GCCAGGTAAA AGCCTCTGGA 
AAAGGTCCCC ATGTCAGAGC CGACTCGGTG TCTGCCAAGG GGATCTCTGG GAAAGGGCCC 
ATCTTAGCAA CCCCAGGGAA GACTGGGCCT GCAGCCACCC AGGCCAAGGC AGAAAGGCCA 
GAGAAGGACT CGGAAACCAG CAGTGAGGAC GATTCTGATA GTGAGGATGA AATGCCAGTC 
ACTGTGAATA CTCCTCAGGC AAGGACTTCT GGGAAGAGCC CTCGGGCCAG AGGTACCTCA 
GCCCCCGCCA AGGAGTCATC CCAGAAAGGG GCTCCTGCAG TCACCCCTGG AAAGGCAAGG 
CCTGTGGCAG CCCAGGCAGG GAAACCAGAA GCCAAGAGCA GTGAGGAGTC AGAGAGTGAC 
AGTGGGGAGA CACCAGCTGC TGCGACTCTG ACCACGAGTC CTGCCAAGGT GAAACCTTTG 
GGGAAGAGCT CCCAGGTCAG ACCTGTTTCC ACCGTCACCC CGGGGTCATC GGGAAAAGGT 
GCCAACCTGC CCTGCCCTGG GAAGGTGGGG TCAGCAGCTC TCAGGGTCCA AATGGTAAAG 
AAAGAAGATG TCTCGGAGAG CAGCAGTGCA GAGCTGGACA GTGACGGGCC TGGGAGCCCA 
GCCAAGGCAA AGGCCTCCCT TGCGCTCCCT CAGAAGGTGA GGCCTGTGGC CACCCAGGTC 
AAGACTGACA GGGGCAAAGG CCACTCAGGG AGCAGTGAGG AGTCATCTGA CAGCGAAGAG 
GAGGCAGCAC CAGCAGCCTC TGCTGCTCAG GCTAAGCCAG CTCTGGAAAA GCAGATGAAA 
GCTTCCTCTA GGAAAGGCAC GCCTGCATCC GCAACAGGAG CGAGCACCTC GTCCCATTGT 
AAGGCAGGAG CGGTGACCTC TTCAGCCAGC CTGTCATCCC CAGCTCTGGC CAAGGGCACC 
CAGAGGTCAG ATGTGGACTC TTCCAGTGAG TCTGAGTCAG AAGGAGCTGC TCCCAGCACC 
CCCAGGGTAC AGGGGAAGTC TGGGGGCAAG GGCCTCCAAG GGAAAGCTGC CTTGGGGCAA 
GGGGTGGCCC CAGTGCACAC TCAGAAGACA GGGCCTTCGG TCAAAGCTAT GGCTCAGGAA 
GACTCAGAGA GCCTCGAGGA GGACTCCAGC AGTGAGGAAG AGGATGAGAC CCCAGCACAG 
GCCACGCCCT TGGGGAGACT TCCTCAGGCC AAAGCCAACC CACCTCCCAC TAAGACACCT 
CCAGCGTCTG CATCTGGAAA AGCTGTGGCT GCTCCAACCA AGGGAAAACC ACCTGTTCCG 
AACAGCACCG TCTCTGCAAG GGGCCAGCGG TCTGTGCCAG CCGCGGGAAA AGCAGGGGCC 
CCAGCAACCC AAGCCCAGAA GGGTCCCGTG GCTGGCACAG GGGAGGACTC AGAGAGCAGC 
AGTAAAGAGG AGTCTGACAG TGAAGAAGAG ACGCCAGCCC AGATAAAACC TGTGGGGAAG 
ACCTCTCAGG TCAGAGCTGC CTCAGCCCCT GCCAAGGAGT CTCCTAAAAA AGGAGCCCAT 
CCAGGAACCC CCGGCAAGAC GGGATCTTCA GCTACCCAGG CCCAGCCAGG GAAGACAGAG 
GACTCAGACA GCAGTAGTGA AGAGTCTGAC AGTGACACAG AGATGCCATC AGCCCAGGAT 
GCTATCTCCC AGCCTGCCAG AGGCAAAGCG TCAGGTCCAG CGTCCCCAGA GAAGAGCATA 
GAAGGGTCCT CAGAGAGCAG TGATGAGGAT CTGCCCTCCG GCCAGGCGAT TAAATCCCCT 
CCAGTTTCTG TCAACCGTAA CAGTAGTCCA GCTGTCCCAG CTCCTACCCC AGAAGGAGTC 
CAGGCTGTGA ACACCACAAA GAAGGCCTCA GGCACCACTG CCCAGAGCTC CTCCTCTGAG 
AGTGAGGACG GGGACGAGGA CTTGATTCCT GCCACACAAC CCTCCACCTA TGCTCTCAGA 
ACCAGTGTGA CGACGCCCGC AGCCCTCTCA CGAGCAGCTT CCCAACCCAG CAAGAGTGAG 
CAGTCTAGCC GGATGCCAAA AGGCAAGAAA GCAAAGGCGG CGGCGTCCGC TCAGACCAGC 
AGTGCCGTGG AAACACTCCC CATGATGCCT CCCCAGAGCG CACCCATCCA GCCCAAAGCC 
ACCAACAAGC TCGGGAAATC CAAGCTCCCT GAGAAGCAGC AGCTTGCCCC AGGCTACCCC 
AAGGCCCCCA GGAGCTCGGA GGACAGCAGT GACACTTCTT CCGAGGACGA GGAGGATGCC 
AAGAGACCCC AGATGCCCAA GTCGGCCCAC AGGCTGGATC CAGACCCTTC CCAGAAGGAA 
ACTGTGGTAG AGGAGACCCC TACAGAATCC AGCGAAGATG AGATGGTGGC CCCCTCACAG 
TCTCTCCTCT CAGGTTACAT GACTCCCGGC TTGACTGTGG CTAATTCCCA GGCTTCAAAA 
GCTACTCCTA GGCCAGACTC CAACTCCTTA GCTTCTTCTG CCCCGGCCAC CAAAGACAAC 
CCGGATGGCA AGCAGAAGTC AAAATCCCAA CACGCAGCAG ACACCGCACT CCCTAAAACC 
GGTAGGAAAG AGGCCTCCTC GGGCTCCACG CCTCAGAAGC CCAAGAAGCT CAAGAAGAGC 
ACCTCAAGCT CGCCGGCCCC CACACAGACA CTGCCGAACA GCATCACCCA GCGCCTCCTG 
GAGCAGGCCT GGCCCCTGAG CGAGGCGCAG GTGCAGGCCT CTGTGGTGAA GGTCCTGACA 
GAGCTTCTGG AGCAGGAACG GCTGAAGGCC ACAGAGGCCA TCAAGGAGAG TGGGAAGAAG 
AGCCAGAAGC GGAAGTTATC AGGGGACCTA GAAGCTGGGG CCCCAAAGAA CAAGAAGAAG 
AAGGAGCAGC CAGTGCCCAG GGCAAGCGCT GTTTCCCCAG AAAAGGCTCC CATGACTTCC 
AAGGCAAAAT CAAAGCTTGA CAAAGGGAGT GCTGGTGGCA AGGGGAAGGG GTCTCCTGGC 
CCCCAAGGAG CCAAGGAGAA GCCCGACGGC GAGTTGCTGG GGATAAAGCT TGAGAGTGGC 
GAGCAGAGCG ACCCGAAGAG CAAGTCGAAA AAGAAGAAAT CCCTCAAGAA AAAAAAAGAC 
AAGGAGAAAA AGGAAAAGAA GAAAGGAAAA AAGTCCCTGG CCAAAGACTC TGCCTCGCCG 
ATCCAGAAGA AGAAAAAGAA GAAGAAGAAG TCAGCCGAGC CTGCCGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001185913.1
CDS	107..4177
Translation	MAEARKRRELLPLIYHHLLQAGYVRAAREVKEQSGQKSFLTQPVTLLDIYTHWQQTSELGQKQKAEDDETLQAKKSRVSDPVSSSESSDQEKEEEAATERAKATPRPTPVNSATAALPSKVKEKGKTKTANKTVNSVSHPGSGKTVVHLLSGKSPKKSAEPLANTVLASETEEEGNAQALGPTAKSGTVSAGQGSSSSEDSSISSDETDVEVKSPAKPAQAKASAAPAKDPPARTAPGPTKLGNVAPTPAKPARAAAAAAAAAVAAAAAAAAEESESSEEDSDSEDEAPAGLPSQVKASGKGPHVRADSVSAKGISGKGPILATPGKTGPAATQAKAERPEKDSETSSEDDSDSEDEMPVTVNTPQARTSGKSPRARGTSAPAKESSQKGAPAVTPGKARPVAAQAGKPEAKSSEESESDSGETPAAATLTTSPAKVKPLGKSSQVRPVSTVTPGSSGKGANLPCPGKVGSAALRVQMVKKEDVSESSSAELDSDGPGSPAKAKASLALPQKVRPVATQVKTDRGKGHSGSSEESSDSEEEAAPAASAAQAKPALEKQMKASSRKGTPASATGASTSSHCKAGAVTSSASLSSPALAKGTQRSDVDSSSESESEGAAPSTPRVQGKSGGKGLQGKAALGQGVAPVHTQKTGPSVKAMAQEDSESLEEDSSSEEEDETPAQATPLGRLPQAKANPPPTKTPPASASGKAVAAPTKGKPPVPNSTVSARGQRSVPAAGKAGAPATQAQKGPVAGTGEDSESSSKEESDSEEETPAQIKPVGKTSQVRAASAPAKESPKKGAHPGTPGKTGSSATQAQPGKTEDSDSSSEESDSDTEMPSAQDAISQPARGKASGPASPEKSIEGSSESSDEDLPSGQAIKSPPVSVNRNSSPAVPAPTPEGVQAVNTTKKASGTTAQSSSSESEDGDEDLIPATQPSTYALRTSVTTPAALSRAASQPSKSEQSSRMPKGKKAKAAASAQTSSAVETLPMMPPQSAPIQPKATNKLGKSKLPEKQQLAPGYPKAPRSSEDSSDTSSEDEEDAKRPQMPKSAHRLDPDPSQKETVVEETPTESSEDEMVAPSQSLLSGYMTPGLTVANSQASKATPRPDSNSLASSAPATKDNPDGKQKSKSQHAADTALPKTGRKEASSGSTPQKPKKLKKSTSSSPAPTQTLPNSITQRLLEQAWPLSEAQVQASVVKVLTELLEQERLKATEAIKESGKKSQKRKLSGDLEAGAPKNKKKKEQPVPRASAVSPEKAPMTSKAKSKLDKGSAGGKGKGSPGPQGAKEKPDGELLGIKLESGEQSDPKSKSKKKKSLKKKKDKEKKEKKKGKKSLAKDSASPIQKKKKKKKKSAEPAV

Target ORF information:

RefSeq Version	NM_001198984.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus treacle ribosome biogenesis factor 1 (Tcof1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001198984.1

ORF Insert Sequence:

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTG CTTCCCCTCA TTTACCATCA TCTGTTGCAA 
GCAGGCTACG TCCGCGCGGC GCGGGAAGTA AAGGAGCAGA GCGGCCAGAA GAGTTTCCTG 
ACTCAGCCCG TCACCCTTCT GGACATCTAT ACACACTGGC AACAGACCTC AGAGCTTGGC 
CAGAAGCAGA AGGCAGAGGA TGATGAGACC CTTCAGGCTA AGAAGTCTCG AGTGTCGGAT 
CCTGTTAGCA GCTCAGAGAG CTCGGATCAG GAGAAGGAAG AGGAGGCAGC AACCGAAAGG 
GCCAAAGCCA CCCCAAGACC GACACCTGTC AATTCTGCAA CCGCAGCTTT GCCATCAAAA 
GTAAAAGAAA AGGGAAAGAC CAAGACAGCC AACAAGACGG TGAACTCTGT GTCGCACCCT 
GGGTCCGGAA AGACGGTGGT CCACCTGCTC TCTGGGAAGT CACCCAAAAA GTCAGCAGAG 
CCCTTGGCAA ACACTGTCTT GGCCTCAGAA ACTGAGGAGG AGGGCAATGC CCAAGCCCTC 
GGACCCACTG CCAAGTCTGG AACGGTGTCA GCGGGCCAAG GCAGCAGTTC CAGTGAAGAT 
TCCTCCATCT CAAGCGATGA GACAGATGTC GAGGTGAAAT CTCCAGCAAA ACCAGCCCAG 
GCCAAAGCTT CAGCAGCCCC TGCCAAGGAT CCTCCAGCAA GAACAGCCCC AGGCCCTACC 
AAGTTAGGGA ATGTGGCGCC CACACCTGCT AAACCAGCCA GGGCGGCAGC GGCAGCTGCT 
GCTGCTGCTG TGGCTGCTGC TGCTGCTGCA GCAGCAGAAG AGTCTGAGAG CAGTGAGGAG 
GACTCAGACA GTGAGGACGA GGCCCCTGCT GGTCTGCCCA GCCAGGTAAA AGCCTCTGGA 
AAAGGTCCCC ATGTCAGAGC CGACTCGGTG TCTGCCAAGG GGATCTCTGG GAAAGGGCCC 
ATCTTAGCAA CCCCAGGGAA GACTGGGCCT GCAGCCACCC AGGCCAAGGC AGAAAGGCCA 
GAGAAGGACT CGGAAACCAG CAGTGAGGAC GATTCTGATA GTGAGGATGA AATGCCAGTC 
ACTGTGAATA CTCCTCAGGC AAGGACTTCT GGGAAGAGCC CTCGGGCCAG AGGTACCTCA 
GCCCCCGCCA AGGAGTCATC CCAGAAAGGG GCTCCTGCAG TCACCCCTGG AAAGGCAAGG 
CCTGTGGCAG CCCAGGCAGG GAAACCAGAA GCCAAGAGCA GTGAGGAGTC AGAGAGTGAC 
AGTGGGGAGA CACCAGCTGC TGCGACTCTG ACCACGAGTC CTGCCAAGGT GAAACCTTTG 
GGGAAGAGCT CCCAGGTCAG ACCTGTTTCC ACCGTCACCC CGGGGTCATC GGGAAAAGGT 
GCCAACCTGC CCTGCCCTGG GAAGGTGGGG TCAGCAGCTC TCAGGGTCCA AATGGTAAAG 
AAAGAAGATG TCTCGGAGAG CAGCAGTGCA GAGCTGGACA GTGACGGGCC TGGGAGCCCA 
GCCAAGGCAA AGGCCTCCCT TGCGCTCCCT CAGAAGGTGA GGCCTGTGGC CACCCAGGTC 
AAGACTGACA GGGGCAAAGG CCACTCAGGG AGCAGTGAGG AGTCATCTGA CAGCGAAGAG 
GAGGCAGCAC CAGCAGCCTC TGCTGCTCAG GCTAAGCCAG CTCTGGAAAA GCAGATGAAA 
GCTTCCTCTA GGAAAGGCAC GCCTGCATCC GCAACAGGAG CGAGCACCTC GTCCCATTGT 
AAGGCAGGAG CGGTGACCTC TTCAGCCAGC CTGTCATCCC CAGCTCTGGC CAAGGGCACC 
CAGAGGTCAG ATGTGGACTC TTCCAGTGAG TCTGAGTCAG AAGGAGCTGC TCCCAGCACC 
CCCAGGGTAC AGGGGAAGTC TGGGGGCAAG GGCCTCCAAG GGAAAGCTGC CTTGGGGCAA 
GGGGTGGCCC CAGTGCACAC TCAGAAGACA GGGCCTTCGG TCAAAGCTAT GGCTCAGGAA 
GACTCAGAGA GCCTCGAGGA GGACTCCAGC AGTGAGGAAG AGGATGAGAC CCCAGCACAG 
GCCACGCCCT TGGGGAGACT TCCTCAGGCC AAAGCCAACC CACCTCCCAC TAAGACACCT 
CCAGCGTCTG CATCTGGAAA AGCTGTGGCT GCTCCAACCA AGGGAAAACC ACCTGTTCCG 
AACAGCACCG TCTCTGCAAG GGGCCAGCGG TCTGTGCCAG CCGCGGGAAA AGCAGGGGCC 
CCAGCAACCC AAGCCCAGAA GGGTCCCGTG GCTGGCACAG GGGAGGACTC AGAGAGCAGC 
AGTAAAGAGG AGTCTGACAG TGAAGAAGAG ACGCCAGCCC AGATAAAACC TGTGGGGAAG 
ACCTCTCAGG TCAGAGCTGC CTCAGCCCCT GCCAAGGAGT CTCCTAAAAA AGGAGCCCAT 
CCAGGAACCC CCGGCAAGAC GGGATCTTCA GCTACCCAGG CCCAGCCAGG GAAGACAGAG 
GACTCAGACA GCAGTAGTGA AGAGTCTGAC AGTGACACAG AGATGCCATC AGCCCAGGAT 
GCTATCTCCC AGCCTGCCAG AGGCAAAGCG TCAGGTCCAG CGTCCCCAGA GAAGAGCATA 
GAAGGGTCCT CAGAGAGCAG TGATGAGGAT CTGCCCTCCG GCCAGGCGAT TAAATCCCCT 
CCAGTTTCTG TCAACCGTAA CAGTAGTCCA GCTGTCCCAG CTCCTACCCC AGAAGGAGTC 
CAGGCTGTGA ACACCACAAA GAAGGCCTCA GGCACCACTG CCCAGAGCTC CTCCTCTGAG 
AGTGAGGACG GGGACGAGGA CTTGATTCCT GCCACACAAC CCTCCACCTA TGCTCTCAGA 
ACCAGTGTGA CGACGCCCGC AGCCCTCTCA CGAGCAGCTT CCCAACCCAG CAAGAGTGAG 
CAGTCTAGCC GGATGCCAAA AGGCAAGAAA GCAAAGGCGG CGGCGTCCGC TCAGACCAGC 
AGTGCCGTGG AAACACTCCC CATGATGCCT CCCCAGAGCG CACCCATCCA GCCCAAAGCC 
ACCAACAAGC TCGGGAAATC CAAGCTCCCT GAGAAGCAGC AGCTTGCCCC AGGCTACCCC 
AAGGCCCCCA GGAGCTCGGA GGACAGCAGT GACACTTCTT CCGAGGACGA GGAGGATGCC 
AAGAGACCCC AGATGCCCAA GTCGGCCCAC AGGCTGGATC CAGACCCTTC CCAGAAGGAA 
ACTGTGGTAG AGGAGACCCC TACAGAATCC AGCGAAGATG AGATGGTGGC CCCCTCACAG 
TCTCTCCTCT CAGGTTACAT GACTCCCGGC TTGACTGTGG CTAATTCCCA GGCTTCAAAA 
GCTACTCCTA GGCCAGACTC CAACTCCTTA GCTTCTTCTG CCCCGGCCAC CAAAGACAAC 
CCGGATGGCA AGCAGAAGTC AAAATCCCAA CACGCAGCAG ACACCGCACT CCCTAAAACC 
GGTAGGAAAG AGGCCTCCTC GGGCTCCACG CCTCAGAAGC CCAAGAAGCT CAAGAAGAGC 
ACCTCAAGCT CGCCGGCCCC CACACAGACA CTGCCGAACA GCATCACCCA GCGCCTCCTG 
GAGCAGGCCT GGCCCCTGAG CGAGGCGCAG GTGCAGGCCT CTGTGGTGAA GGTCCTGACA 
GAGCTTCTGG AGCAGGAACG GCTGAAGGCC ACAGAGGCCA TCAAGGAGAG TGGGAAGAAG 
AGCCAGAAGC GGAAGTTATC AGGGGACCTA GAAGCTGGGG CCCCAAAGAA CAAGAAGAAG 
AAGGAGCAGC CAGTGCCCAG GGCAAGCGCT GTTTCCCCAG AAAAGGCTCC CATGACTTCC 
AAGGCAAAAT CAAAGCTTGA CAAAGGGAGT GCTGGTGGCA AGGGGAAGGG GTCTCCTGGC 
CCCCAAGGAG CCAAGGAGAA GCCCGACGGC GAGTTGCTGG GGATAAAGCT TGAGAGTGGC 
GAGCAGAGCG ACCCGAAGAG CAAGTCGAAA AAGAAGAAAT CCCTCAAGAA AAAAAAAGAC 
AAGGAGAAAA AGGAAAAGAA GAAAGGAAAA AAGTCCCTGG CCAAAGACTC TGCCTCGCCG 
ATCCAGAAGA AGAAAAAGAA GAAGAAGAAG TCAGCCGAGC CTGCCGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tcof1 ( NM_011552.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035682.1 Mus musculus treacle ribosome biogenesis factor 1 (Tcof1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu16275
Clone ID Related Accession (Same CDS sequence)	NM_011552.3
Accession Version	NM_011552.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3963bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	treacle protein isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC139759.4 and BC057342.1. On Nov 17, 2010 this sequence version replaced NM_011552.2. Transcript Variant: This variant (2) lacks an in-frame alternate exon in the 3' coding region, compared to variant 1, and encodes a shorter isoform (2), compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AF001794.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTG CTTCCCCTCA TTTACCATCA TCTGTTGCAA 
GCAGGCTACG TCCGCGCGGC GCGGGAAGTA AAGGAGCAGA GCGGCCAGAA GAGTTTCCTG 
ACTCAGCCCG TCACCCTTCT GGACATCTAT ACACACTGGC AACAGACCTC AGAGCTTGGC 
CAGAAGCAGA AGGCAGAGGA TGATGAGACC CTTCAGGCTA AGAAGTCTCG AGTGTCGGAT 
CCTGTTAGCA GCTCAGAGAG CTCGGATCAG GAGAAGGAAG AGGAGGCAGC AACCGAAAGG 
GCCAAAGCCA CCCCAAGACC GACACCTGTC AATTCTGCAA CCGCAGCTTT GCCATCAAAA 
GTAAAAGAAA AGGGAAAGAC CAAGACAGCC AACAAGACGG TGAACTCTGT GTCGCACCCT 
GGGTCCGGAA AGACGGTGGT CCACCTGCTC TCTGGGAAGT CACCCAAAAA GTCAGCAGAG 
CCCTTGGCAA ACACTGTCTT GGCCTCAGAA ACTGAGGAGG AGGGCAATGC CCAAGCCCTC 
GGACCCACTG CCAAGTCTGG AACGGTGTCA GCGGGCCAAG GCAGCAGTTC CAGTGAAGAT 
TCCTCCATCT CAAGCGATGA GACAGATGTC GAGGTGAAAT CTCCAGCAAA ACCAGCCCAG 
GCCAAAGCTT CAGCAGCCCC TGCCAAGGAT CCTCCAGCAA GAACAGCCCC AGGCCCTACC 
AAGTTAGGGA ATGTGGCGCC CACACCTGCT AAACCAGCCA GGGCGGCAGC GGCAGCTGCT 
GCTGCTGCTG TGGCTGCTGC TGCTGCTGCA GCAGCAGAAG AGTCTGAGAG CAGTGAGGAG 
GACTCAGACA GTGAGGACGA GGCCCCTGCT GGTCTGCCCA GCCAGGTAAA AGCCTCTGGA 
AAAGGTCCCC ATGTCAGAGC CGACTCGGTG TCTGCCAAGG GGATCTCTGG GAAAGGGCCC 
ATCTTAGCAA CCCCAGGGAA GACTGGGCCT GCAGCCACCC AGGCCAAGGC AGAAAGGCCA 
GAGAAGGACT CGGAAACCAG CAGTGAGGAC GATTCTGATA GTGAGGATGA AATGCCAGTC 
ACTGTGAATA CTCCTCAGGC AAGGACTTCT GGGAAGAGCC CTCGGGCCAG AGGTACCTCA 
GCCCCCGCCA AGGAGTCATC CCAGAAAGGG GCTCCTGCAG TCACCCCTGG AAAGGCAAGG 
CCTGTGGCAG CCCAGGCAGG GAAACCAGAA GCCAAGAGCA GTGAGGAGTC AGAGAGTGAC 
AGTGGGGAGA CACCAGCTGC TGCGACTCTG ACCACGAGTC CTGCCAAGGT GAAACCTTTG 
GGGAAGAGCT CCCAGGTCAG ACCTGTTTCC ACCGTCACCC CGGGGTCATC GGGAAAAGGT 
GCCAACCTGC CCTGCCCTGG GAAGGTGGGG TCAGCAGCTC TCAGGGTCCA AATGGTAAAG 
AAAGAAGATG TCTCGGAGAG CAGCAGTGCA GAGCTGGACA GTGACGGGCC TGGGAGCCCA 
GCCAAGGCAA AGGCCTCCCT TGCGCTCCCT CAGAAGGTGA GGCCTGTGGC CACCCAGGTC 
AAGACTGACA GGGGCAAAGG CCACTCAGGG AGCAGTGAGG AGTCATCTGA CAGCGAAGAG 
GAGGCAGCAC CAGCAGCCTC TGCTGCTCAG GCTAAGCCAG CTCTGGAAAA GCAGATGAAA 
GCTTCCTCTA GGAAAGGCAC GCCTGCATCC GCAACAGGAG CGAGCACCTC GTCCCATTGT 
AAGGCAGGAG CGGTGACCTC TTCAGCCAGC CTGTCATCCC CAGCTCTGGC CAAGGGCACC 
CAGAGGTCAG ATGTGGACTC TTCCAGTGAG TCTGAGTCAG AAGGAGCTGC TCCCAGCACC 
CCCAGGGTAC AGGGGAAGTC TGGGGGCAAG GGCCTCCAAG GGAAAGCTGC CTTGGGGCAA 
GGGGTGGCCC CAGTGCACAC TCAGAAGACA GGGCCTTCGG TCAAAGCTAT GGCTCAGGAA 
GACTCAGAGA GCCTCGAGGA GGACTCCAGC AGTGAGGAAG AGGATGAGAC CCCAGCACAG 
GCCACGCCCT TGGGGAGACT TCCTCAGGCC AAAGCCAACC CACCTCCCAC TAAGACACCT 
CCAGCGTCTG CATCTGGAAA AGCTGTGGCT GCTCCAACCA AGGGAAAACC ACCTGTTCCG 
AACAGCACCG TCTCTGCAAG GGGCCAGCGG TCTGTGCCAG CCGCGGGAAA AGCAGGGGCC 
CCAGCAACCC AAGCCCAGAA GGGTCCCGTG GCTGGCACAG GGGAGGACTC AGAGAGCAGC 
AGTAAAGAGG AGTCTGACAG TGAAGAAGAG ACGCCAGCCC AGATAAAACC TGTGGGGAAG 
ACCTCTCAGG TCAGAGCTGC CTCAGCCCCT GCCAAGGAGT CTCCTAAAAA AGGAGCCCAT 
CCAGGAACCC CCGGCAAGAC GGGATCTTCA GCTACCCAGG CCCAGCCAGG GAAGACAGAG 
GACTCAGACA GCAGTAGTGA AGAGTCTGAC AGTGACACAG AGATGCCATC AGCCCAGGCG 
ATTAAATCCC CTCCAGTTTC TGTCAACCGT AACAGTAGTC CAGCTGTCCC AGCTCCTACC 
CCAGAAGGAG TCCAGGCTGT GAACACCACA AAGAAGGCCT CAGGCACCAC TGCCCAGAGC 
TCCTCCTCTG AGAGTGAGGA CGGGGACGAG GACTTGATTC CTGCCACACA ACCCTCCACC 
TATGCTCTCA GAACCAGTGT GACGACGCCC GCAGCCCTCT CACGAGCAGC TTCCCAACCC 
AGCAAGAGTG AGCAGTCTAG CCGGATGCCA AAAGGCAAGA AAGCAAAGGC GGCGGCGTCC 
GCTCAGACCA GCAGTGCCGT GGAAACACTC CCCATGATGC CTCCCCAGAG CGCACCCATC 
CAGCCCAAAG CCACCAACAA GCTCGGGAAA TCCAAGCTCC CTGAGAAGCA GCAGCTTGCC 
CCAGGCTACC CCAAGGCCCC CAGGAGCTCG GAGGACAGCA GTGACACTTC TTCCGAGGAC 
GAGGAGGATG CCAAGAGACC CCAGATGCCC AAGTCGGCCC ACAGGCTGGA TCCAGACCCT 
TCCCAGAAGG AAACTGTGGT AGAGGAGACC CCTACAGAAT CCAGCGAAGA TGAGATGGTG 
GCCCCCTCAC AGTCTCTCCT CTCAGGTTAC ATGACTCCCG GCTTGACTGT GGCTAATTCC 
CAGGCTTCAA AAGCTACTCC TAGGCCAGAC TCCAACTCCT TAGCTTCTTC TGCCCCGGCC 
ACCAAAGACA ACCCGGATGG CAAGCAGAAG TCAAAATCCC AACACGCAGC AGACACCGCA 
CTCCCTAAAA CCGGTAGGAA AGAGGCCTCC TCGGGCTCCA CGCCTCAGAA GCCCAAGAAG 
CTCAAGAAGA GCACCTCAAG CTCGCCGGCC CCCACACAGA CACTGCCGAA CAGCATCACC 
CAGCGCCTCC TGGAGCAGGC CTGGCCCCTG AGCGAGGCGC AGGTGCAGGC CTCTGTGGTG 
AAGGTCCTGA CAGAGCTTCT GGAGCAGGAA CGGCTGAAGG CCACAGAGGC CATCAAGGAG 
AGTGGGAAGA AGAGCCAGAA GCGGAAGTTA TCAGGGGACC TAGAAGCTGG GGCCCCAAAG 
AACAAGAAGA AGAAGGAGCA GCCAGTGCCC AGGGCAAGCG CTGTTTCCCC AGAAAAGGCT 
CCCATGACTT CCAAGGCAAA ATCAAAGCTT GACAAAGGGA GTGCTGGTGG CAAGGGGAAG 
GGGTCTCCTG GCCCCCAAGG AGCCAAGGAG AAGCCCGACG GCGAGTTGCT GGGGATAAAG 
CTTGAGAGTG GCGAGCAGAG CGACCCGAAG AGCAAGTCGA AAAAGAAGAA ATCCCTCAAG 
AAAAAAAAAG ACAAGGAGAA AAAGGAAAAG AAGAAAGGAA AAAAGTCCCT GGCCAAAGAC 
TCTGCCTCGC CGATCCAGAA GAAGAAAAAG AAGAAGAAGA AGTCAGCCGA GCCTGCCGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035682.1
CDS	107..4069
Translation	MAEARKRRELLPLIYHHLLQAGYVRAAREVKEQSGQKSFLTQPVTLLDIYTHWQQTSELGQKQKAEDDETLQAKKSRVSDPVSSSESSDQEKEEEAATERAKATPRPTPVNSATAALPSKVKEKGKTKTANKTVNSVSHPGSGKTVVHLLSGKSPKKSAEPLANTVLASETEEEGNAQALGPTAKSGTVSAGQGSSSSEDSSISSDETDVEVKSPAKPAQAKASAAPAKDPPARTAPGPTKLGNVAPTPAKPARAAAAAAAAAVAAAAAAAAEESESSEEDSDSEDEAPAGLPSQVKASGKGPHVRADSVSAKGISGKGPILATPGKTGPAATQAKAERPEKDSETSSEDDSDSEDEMPVTVNTPQARTSGKSPRARGTSAPAKESSQKGAPAVTPGKARPVAAQAGKPEAKSSEESESDSGETPAAATLTTSPAKVKPLGKSSQVRPVSTVTPGSSGKGANLPCPGKVGSAALRVQMVKKEDVSESSSAELDSDGPGSPAKAKASLALPQKVRPVATQVKTDRGKGHSGSSEESSDSEEEAAPAASAAQAKPALEKQMKASSRKGTPASATGASTSSHCKAGAVTSSASLSSPALAKGTQRSDVDSSSESESEGAAPSTPRVQGKSGGKGLQGKAALGQGVAPVHTQKTGPSVKAMAQEDSESLEEDSSSEEEDETPAQATPLGRLPQAKANPPPTKTPPASASGKAVAAPTKGKPPVPNSTVSARGQRSVPAAGKAGAPATQAQKGPVAGTGEDSESSSKEESDSEEETPAQIKPVGKTSQVRAASAPAKESPKKGAHPGTPGKTGSSATQAQPGKTEDSDSSSEESDSDTEMPSAQAIKSPPVSVNRNSSPAVPAPTPEGVQAVNTTKKASGTTAQSSSSESEDGDEDLIPATQPSTYALRTSVTTPAALSRAASQPSKSEQSSRMPKGKKAKAAASAQTSSAVETLPMMPPQSAPIQPKATNKLGKSKLPEKQQLAPGYPKAPRSSEDSSDTSSEDEEDAKRPQMPKSAHRLDPDPSQKETVVEETPTESSEDEMVAPSQSLLSGYMTPGLTVANSQASKATPRPDSNSLASSAPATKDNPDGKQKSKSQHAADTALPKTGRKEASSGSTPQKPKKLKKSTSSSPAPTQTLPNSITQRLLEQAWPLSEAQVQASVVKVLTELLEQERLKATEAIKESGKKSQKRKLSGDLEAGAPKNKKKKEQPVPRASAVSPEKAPMTSKAKSKLDKGSAGGKGKGSPGPQGAKEKPDGELLGIKLESGEQSDPKSKSKKKKSLKKKKDKEKKEKKKGKKSLAKDSASPIQKKKKKKKKSAEPAV

Target ORF information:

RefSeq Version	NM_011552.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus treacle ribosome biogenesis factor 1 (Tcof1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011552.3

ORF Insert Sequence:

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTG CTTCCCCTCA TTTACCATCA TCTGTTGCAA 
GCAGGCTACG TCCGCGCGGC GCGGGAAGTA AAGGAGCAGA GCGGCCAGAA GAGTTTCCTG 
ACTCAGCCCG TCACCCTTCT GGACATCTAT ACACACTGGC AACAGACCTC AGAGCTTGGC 
CAGAAGCAGA AGGCAGAGGA TGATGAGACC CTTCAGGCTA AGAAGTCTCG AGTGTCGGAT 
CCTGTTAGCA GCTCAGAGAG CTCGGATCAG GAGAAGGAAG AGGAGGCAGC AACCGAAAGG 
GCCAAAGCCA CCCCAAGACC GACACCTGTC AATTCTGCAA CCGCAGCTTT GCCATCAAAA 
GTAAAAGAAA AGGGAAAGAC CAAGACAGCC AACAAGACGG TGAACTCTGT GTCGCACCCT 
GGGTCCGGAA AGACGGTGGT CCACCTGCTC TCTGGGAAGT CACCCAAAAA GTCAGCAGAG 
CCCTTGGCAA ACACTGTCTT GGCCTCAGAA ACTGAGGAGG AGGGCAATGC CCAAGCCCTC 
GGACCCACTG CCAAGTCTGG AACGGTGTCA GCGGGCCAAG GCAGCAGTTC CAGTGAAGAT 
TCCTCCATCT CAAGCGATGA GACAGATGTC GAGGTGAAAT CTCCAGCAAA ACCAGCCCAG 
GCCAAAGCTT CAGCAGCCCC TGCCAAGGAT CCTCCAGCAA GAACAGCCCC AGGCCCTACC 
AAGTTAGGGA ATGTGGCGCC CACACCTGCT AAACCAGCCA GGGCGGCAGC GGCAGCTGCT 
GCTGCTGCTG TGGCTGCTGC TGCTGCTGCA GCAGCAGAAG AGTCTGAGAG CAGTGAGGAG 
GACTCAGACA GTGAGGACGA GGCCCCTGCT GGTCTGCCCA GCCAGGTAAA AGCCTCTGGA 
AAAGGTCCCC ATGTCAGAGC CGACTCGGTG TCTGCCAAGG GGATCTCTGG GAAAGGGCCC 
ATCTTAGCAA CCCCAGGGAA GACTGGGCCT GCAGCCACCC AGGCCAAGGC AGAAAGGCCA 
GAGAAGGACT CGGAAACCAG CAGTGAGGAC GATTCTGATA GTGAGGATGA AATGCCAGTC 
ACTGTGAATA CTCCTCAGGC AAGGACTTCT GGGAAGAGCC CTCGGGCCAG AGGTACCTCA 
GCCCCCGCCA AGGAGTCATC CCAGAAAGGG GCTCCTGCAG TCACCCCTGG AAAGGCAAGG 
CCTGTGGCAG CCCAGGCAGG GAAACCAGAA GCCAAGAGCA GTGAGGAGTC AGAGAGTGAC 
AGTGGGGAGA CACCAGCTGC TGCGACTCTG ACCACGAGTC CTGCCAAGGT GAAACCTTTG 
GGGAAGAGCT CCCAGGTCAG ACCTGTTTCC ACCGTCACCC CGGGGTCATC GGGAAAAGGT 
GCCAACCTGC CCTGCCCTGG GAAGGTGGGG TCAGCAGCTC TCAGGGTCCA AATGGTAAAG 
AAAGAAGATG TCTCGGAGAG CAGCAGTGCA GAGCTGGACA GTGACGGGCC TGGGAGCCCA 
GCCAAGGCAA AGGCCTCCCT TGCGCTCCCT CAGAAGGTGA GGCCTGTGGC CACCCAGGTC 
AAGACTGACA GGGGCAAAGG CCACTCAGGG AGCAGTGAGG AGTCATCTGA CAGCGAAGAG 
GAGGCAGCAC CAGCAGCCTC TGCTGCTCAG GCTAAGCCAG CTCTGGAAAA GCAGATGAAA 
GCTTCCTCTA GGAAAGGCAC GCCTGCATCC GCAACAGGAG CGAGCACCTC GTCCCATTGT 
AAGGCAGGAG CGGTGACCTC TTCAGCCAGC CTGTCATCCC CAGCTCTGGC CAAGGGCACC 
CAGAGGTCAG ATGTGGACTC TTCCAGTGAG TCTGAGTCAG AAGGAGCTGC TCCCAGCACC 
CCCAGGGTAC AGGGGAAGTC TGGGGGCAAG GGCCTCCAAG GGAAAGCTGC CTTGGGGCAA 
GGGGTGGCCC CAGTGCACAC TCAGAAGACA GGGCCTTCGG TCAAAGCTAT GGCTCAGGAA 
GACTCAGAGA GCCTCGAGGA GGACTCCAGC AGTGAGGAAG AGGATGAGAC CCCAGCACAG 
GCCACGCCCT TGGGGAGACT TCCTCAGGCC AAAGCCAACC CACCTCCCAC TAAGACACCT 
CCAGCGTCTG CATCTGGAAA AGCTGTGGCT GCTCCAACCA AGGGAAAACC ACCTGTTCCG 
AACAGCACCG TCTCTGCAAG GGGCCAGCGG TCTGTGCCAG CCGCGGGAAA AGCAGGGGCC 
CCAGCAACCC AAGCCCAGAA GGGTCCCGTG GCTGGCACAG GGGAGGACTC AGAGAGCAGC 
AGTAAAGAGG AGTCTGACAG TGAAGAAGAG ACGCCAGCCC AGATAAAACC TGTGGGGAAG 
ACCTCTCAGG TCAGAGCTGC CTCAGCCCCT GCCAAGGAGT CTCCTAAAAA AGGAGCCCAT 
CCAGGAACCC CCGGCAAGAC GGGATCTTCA GCTACCCAGG CCCAGCCAGG GAAGACAGAG 
GACTCAGACA GCAGTAGTGA AGAGTCTGAC AGTGACACAG AGATGCCATC AGCCCAGGCG 
ATTAAATCCC CTCCAGTTTC TGTCAACCGT AACAGTAGTC CAGCTGTCCC AGCTCCTACC 
CCAGAAGGAG TCCAGGCTGT GAACACCACA AAGAAGGCCT CAGGCACCAC TGCCCAGAGC 
TCCTCCTCTG AGAGTGAGGA CGGGGACGAG GACTTGATTC CTGCCACACA ACCCTCCACC 
TATGCTCTCA GAACCAGTGT GACGACGCCC GCAGCCCTCT CACGAGCAGC TTCCCAACCC 
AGCAAGAGTG AGCAGTCTAG CCGGATGCCA AAAGGCAAGA AAGCAAAGGC GGCGGCGTCC 
GCTCAGACCA GCAGTGCCGT GGAAACACTC CCCATGATGC CTCCCCAGAG CGCACCCATC 
CAGCCCAAAG CCACCAACAA GCTCGGGAAA TCCAAGCTCC CTGAGAAGCA GCAGCTTGCC 
CCAGGCTACC CCAAGGCCCC CAGGAGCTCG GAGGACAGCA GTGACACTTC TTCCGAGGAC 
GAGGAGGATG CCAAGAGACC CCAGATGCCC AAGTCGGCCC ACAGGCTGGA TCCAGACCCT 
TCCCAGAAGG AAACTGTGGT AGAGGAGACC CCTACAGAAT CCAGCGAAGA TGAGATGGTG 
GCCCCCTCAC AGTCTCTCCT CTCAGGTTAC ATGACTCCCG GCTTGACTGT GGCTAATTCC 
CAGGCTTCAA AAGCTACTCC TAGGCCAGAC TCCAACTCCT TAGCTTCTTC TGCCCCGGCC 
ACCAAAGACA ACCCGGATGG CAAGCAGAAG TCAAAATCCC AACACGCAGC AGACACCGCA 
CTCCCTAAAA CCGGTAGGAA AGAGGCCTCC TCGGGCTCCA CGCCTCAGAA GCCCAAGAAG 
CTCAAGAAGA GCACCTCAAG CTCGCCGGCC CCCACACAGA CACTGCCGAA CAGCATCACC 
CAGCGCCTCC TGGAGCAGGC CTGGCCCCTG AGCGAGGCGC AGGTGCAGGC CTCTGTGGTG 
AAGGTCCTGA CAGAGCTTCT GGAGCAGGAA CGGCTGAAGG CCACAGAGGC CATCAAGGAG 
AGTGGGAAGA AGAGCCAGAA GCGGAAGTTA TCAGGGGACC TAGAAGCTGG GGCCCCAAAG 
AACAAGAAGA AGAAGGAGCA GCCAGTGCCC AGGGCAAGCG CTGTTTCCCC AGAAAAGGCT 
CCCATGACTT CCAAGGCAAA ATCAAAGCTT GACAAAGGGA GTGCTGGTGG CAAGGGGAAG 
GGGTCTCCTG GCCCCCAAGG AGCCAAGGAG AAGCCCGACG GCGAGTTGCT GGGGATAAAG 
CTTGAGAGTG GCGAGCAGAG CGACCCGAAG AGCAAGTCGA AAAAGAAGAA ATCCCTCAAG 
AAAAAAAAAG ACAAGGAGAA AAAGGAAAAG AAGAAAGGAA AAAAGTCCCT GGCCAAAGAC 
TCTGCCTCGC CGATCCAGAA GAAGAAAAAG AAGAAGAAGA AGTCAGCCGA GCCTGCCGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tcof1 ( XM_006525777.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006525840.1 Mus musculus treacle ribosome biogenesis factor 1 (Tcof1), transcript variant X1, mRNA.

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CloneID	OMu54472
Clone ID Related Accession (Same CDS sequence)	XM_006525777.2
Accession Version	XM_006525777.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4068bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	treacle protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006525777.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTG CTTCCCCTCA TTTACCATCA TCTGTTGCAA 
GCAGGCTACG TCCGCGCGGC GCGGGAAGTA AAGGAGCAGA GCGGCCAGAA GAGTTTCCTG 
ACTCAGCCCG TCACCCTTCT GGACATCTAT ACACACTGGC AACAGACCTC AGAGCTTGGC 
CAGAAGCAGA AGGCAGAGGA TGATGAGACC CTTCAGGCTA AGAAGTCTCG AGTGTCGGAT 
CCTGTTAGCA GCTCAGAGAG CTCGGATCAG GAGAAGGAAG AGGAGGCAGC AACCGAAAGG 
GCCAAAGCCA CCCCAAGACC GACACCTGTC AATTCTGCAA CCGCAGCTTT GCCATCAAAA 
GTAAAAGAAA AGGGAAAGAC CAAGACAGCC AACAAGACGG TGAACTCTGT GTCGCACCCT 
GGGTCCGGAA AGACGGTGGT CCACCTGCTC TCTGGGAAGT CACCCAAAAA GTCAGCAGAG 
CCCTTGGCAA ACACTGTCTT GGCCTCAGAA ACTGAGGAGG AGGGCAATGC CCAAGCCCTC 
GGACCCACTG CCAAGTCTGG AACGGTGTCA GCGGGCCAAG GCAGCAGTTC CAGTGAAGAT 
TCCTCCATCT CAAGCGATGA GACAGATGTC GAGGTGAAAT CTCCAGCAAA ACCAGCCCAG 
GCCAAAGCTT CAGCAGCCCC TGCCAAGGAT CCTCCAGCAA GAACAGCCCC AGGCCCTACC 
AAGTTAGGGA ATGTGGCGCC CACACCTGCT AAACCAGCCA GGGCGGCAGC GGCAGCTGCT 
GCTGCTGCTG TGGCTGCTGC TGCTGCTGCA GCAGCAGAAG AGTCTGAGAG CAGTGAGGAG 
GACTCAGACA GTGAGGACGA GGCCCCTGCT GGTCTGCCCA GCCAGGTAAA AGCCTCTGGA 
AAAGGTCCCC ATGTCAGAGC CGACTCGGTG TCTGCCAAGG GGATCTCTGG GAAAGGGCCC 
ATCTTAGCAA CCCCAGGGAA GACTGGGCCT GCAGCCACCC AGGCCAAGGC AGAAAGGCCA 
GAGAAGGACT CGGAAACCAG CAGTGAGGAC GATTCTGATA GTGAGGATGA AATGCCAGTC 
ACTGTGAATA CTCCTCAGGC AAGGACTTCT GGGAAGAGCC CTCGGGCCAG AGGTACCTCA 
GCCCCCGCCA AGGAGTCATC CCAGAAAGGG GCTCCTGCAG TCACCCCTGG AAAGGCAAGG 
CCTGTGGCAG CCCAGGCAGG GAAACCAGAA GCCAAGAGCA GTGAGGAGTC AGAGAGTGAC 
AGTGGGGAGA CACCAGCTGC TGCGACTCTG ACCACGAGTC CTGCCAAGGT GAAACCTTTG 
GGGAAGAGCT CCCAGGTCAG ACCTGTTTCC ACCGTCACCC CGGGGTCATC GGGAAAAGGT 
GCCAACCTGC CCTGCCCTGG GAAGGTGGGG TCAGCAGCTC TCAGGGTCCA AATGGTAAAG 
AAAGAAGATG TCTCGGAGAG CAGCAGTGCA GAGCTGGACA GTGACGGGCC TGGGAGCCCA 
GCCAAGGCAA AGGCCTCCCT TGCGCTCCCT CAGAAGGTGA GGCCTGTGGC CACCCAGGTC 
AAGACTGACA GGGGCAAAGG CCACTCAGGG AGCAGTGAGG AGTCATCTGA CAGCGAAGAG 
GAGGCAGCAC CAGCAGCCTC TGCTGCTCAG GCTAAGCCAG CTCTGGAAAA GCAGATGAAA 
GCTTCCTCTA GGAAAGGCAC GCCTGCATCC GCAACAGGAG CGAGCACCTC GTCCCATTGT 
AAGGCAGGAG CGGTGACCTC TTCAGCCAGC CTGTCATCCC CAGCTCTGGC CAAGGGCACC 
CAGAGGTCAG ATGTGGACTC TTCCAGTGAG TCTGAGTCAG AAGGAGCTGC TCCCAGCACC 
CCCAGGGTAC AGGGGAAGTC TGGGGGCAAG GGCCTCCAAG GGAAAGCTGC CTTGGGGCAA 
GGGGTGGCCC CAGTGCACAC TCAGAAGACA GGGCCTTCGG TCAAAGCTAT GGCTCAGGAA 
GACTCAGAGA GCCTCGAGGA GGACTCCAGC AGTGAGGAAG AGGATGAGAC CCCAGCACAG 
GCCACGCCCT TGGGGAGACT TCCTCAGGCC AAAGCCAACC CACCTCCCAC TAAGACACCT 
CCAGCGTCTG CATCTGGAAA AGCTGTGGCT GCTCCAACCA AGGGAAAACC ACCTGTTCCG 
AACAGCACCG TCTCTGCAAG GGGCCAGCGG TCTGTGCCAG CCGCGGGAAA AGCAGGGGCC 
CCAGCAACCC AAGCCCAGAA GGGTCCCGTG GCTGGCACAG GGGAGGACTC AGAGAGCAGC 
AGTAAAGAGG AGTCTGACAG TGAAGAAGAG ACGCCAGCCC AGATAAAACC TGTGGGGAAG 
ACCTCTCAGG TCAGAGCTGC CTCAGCCCCT GCCAAGGAGT CTCCTAAAAA AGGAGCCCAT 
CCAGGAACCC CCGGCAAGAC GGGATCTTCA GCTACCCAGG CCCAGCCAGG GAAGACAGAG 
GACTCAGACA GCAGTAGTGA AGAGTCTGAC AGTGACACAG AGATGCCATC AGCCCAGGAT 
GCTATCTCCC AGCCTGCCAG AGGCAAAGCG TCAGGTCCAG CGTCCCCAGA GAAGAGCATA 
GAAGGGTCCT CAGAGAGCAG TGATGAGGAT CTGCCCTCCG GCCAGGCGAT TAAATCCCCT 
CCAGTTTCTG TCAACCGTAA CAGTAGTCCA GCTGTCCCAG CTCCTACCCC AGAAGGAGTC 
CAGGCTGTGA ACACCACAAA GAAGGCCTCA GGCACCACTG CCCAGAGCTC CTCCTCTGAG 
AGTGAGGACG GGGACGAGGA CTTGATTCCT GCCACACAAC CCTCCACCTA TGCTCTCAGA 
ACCAGTGTGA CGACGCCCGC AGCCCTCTCA CGAGCAGCTT CCCAACCCAG CAAGAGTGAG 
CAGTCTAGCC GGATGCCAAA AGGCAAGAAA GCAAAGGCGG CGGCGTCCGC TCAGACCAGC 
AGTGCCGTGG AAACACTCCC CATGATGCCT CCCCAGAGCG CACCCATCCA GCCCAAAGCC 
ACCAACAAGC TCGGGAAATC CAAGCTCCCT GAGAAGCAGC AGCTTGCCCC AGGCTACCCC 
AAGGCCCCCA GGAGCTCGGA GGACAGCAGT GACACTTCTT CCGAGGACGA GGAGGATGCC 
AAGAGACCCC AGATGCCCAA GTCGGCCCAC AGGCTGGATC CAGACCCTTC CCAGAAGGAA 
ACTGTGGTAG AGGAGACCCC TACAGAATCC AGCGAAGATG AGATGGTGGC CCCCTCACAG 
TCTCTCCTCT CAGGTTACAT GACTCCCGGC TTGACTGTGG CTAATTCCCA GGCTTCAAAA 
GCTACTCCTA GGCCAGACTC CAACTCCTTA GCTTCTTCTG CCCCGGCCAC CAAAGACAAC 
CCGGATGGCA AGCAGAAGTC AAAATCCCAA CACGCAGCAG ACACCGCACT CCCTAAAACC 
GGTAGGAAAG AGGCCTCCTC GGGCTCCACG CCTCAGAAGC CCAAGAAGCT CAAGAAGAGC 
ACCTCAAGCT CGCCGGCCCC CACACAGACA CTGCCGAACA GCATCACCCA GCGCCTCCTG 
GAGCAGGCCT GGCCCCTGAG CGAGGCGCAG GTGCAGGCCT CTGTGGTGAA GGTCCTGACA 
GAGCTTCTGG AGCAGGAACG GCTGAAGGCC ACAGAGGCCA TCAAGGAGAG TGGGAAGAAG 
AGCCAGAAGC GGAAGTTATC AGGGGACCTA GAAGCTGGGG CCCCAAAGAA CAAGAAGAAG 
AAGGAGCAGC CAGTGCCCAG GGCAAGCGCT GTTTCCCCAG AAAAGGCTCC CATGACTTCC 
AAGGCAAAAT CAAAGCTTGA CAAAGGGAGT GCTGGTGGCA AGGGGAAGGG GTCTCCTGGC 
CCCCAAGGAG CCAAGGAGAA GCCCGACGGC GAGTTGCTGG GGATAAAGCT TGAGAGTGGC 
GAGCAGAGCG ACCCGAAGAG CAAGTCGAAA AAGAAGAAAT CCCTCAAGAA AAAAAAAGAC 
AAGGAGAAAA AGGAAAAGAA GAAAGGAAAA AAGTCCCTGG CCAAAGACTC TGCCTCGCCG 
ATCCAGAAGA AGAAAAAGAA GAAGAAGTCA GCCGAGCCTG CCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006525840.1
CDS	126..4193
Translation	MAEARKRRELLPLIYHHLLQAGYVRAAREVKEQSGQKSFLTQPVTLLDIYTHWQQTSELGQKQKAEDDETLQAKKSRVSDPVSSSESSDQEKEEEAATERAKATPRPTPVNSATAALPSKVKEKGKTKTANKTVNSVSHPGSGKTVVHLLSGKSPKKSAEPLANTVLASETEEEGNAQALGPTAKSGTVSAGQGSSSSEDSSISSDETDVEVKSPAKPAQAKASAAPAKDPPARTAPGPTKLGNVAPTPAKPARAAAAAAAAAVAAAAAAAAEESESSEEDSDSEDEAPAGLPSQVKASGKGPHVRADSVSAKGISGKGPILATPGKTGPAATQAKAERPEKDSETSSEDDSDSEDEMPVTVNTPQARTSGKSPRARGTSAPAKESSQKGAPAVTPGKARPVAAQAGKPEAKSSEESESDSGETPAAATLTTSPAKVKPLGKSSQVRPVSTVTPGSSGKGANLPCPGKVGSAALRVQMVKKEDVSESSSAELDSDGPGSPAKAKASLALPQKVRPVATQVKTDRGKGHSGSSEESSDSEEEAAPAASAAQAKPALEKQMKASSRKGTPASATGASTSSHCKAGAVTSSASLSSPALAKGTQRSDVDSSSESESEGAAPSTPRVQGKSGGKGLQGKAALGQGVAPVHTQKTGPSVKAMAQEDSESLEEDSSSEEEDETPAQATPLGRLPQAKANPPPTKTPPASASGKAVAAPTKGKPPVPNSTVSARGQRSVPAAGKAGAPATQAQKGPVAGTGEDSESSSKEESDSEEETPAQIKPVGKTSQVRAASAPAKESPKKGAHPGTPGKTGSSATQAQPGKTEDSDSSSEESDSDTEMPSAQDAISQPARGKASGPASPEKSIEGSSESSDEDLPSGQAIKSPPVSVNRNSSPAVPAPTPEGVQAVNTTKKASGTTAQSSSSESEDGDEDLIPATQPSTYALRTSVTTPAALSRAASQPSKSEQSSRMPKGKKAKAAASAQTSSAVETLPMMPPQSAPIQPKATNKLGKSKLPEKQQLAPGYPKAPRSSEDSSDTSSEDEEDAKRPQMPKSAHRLDPDPSQKETVVEETPTESSEDEMVAPSQSLLSGYMTPGLTVANSQASKATPRPDSNSLASSAPATKDNPDGKQKSKSQHAADTALPKTGRKEASSGSTPQKPKKLKKSTSSSPAPTQTLPNSITQRLLEQAWPLSEAQVQASVVKVLTELLEQERLKATEAIKESGKKSQKRKLSGDLEAGAPKNKKKKEQPVPRASAVSPEKAPMTSKAKSKLDKGSAGGKGKGSPGPQGAKEKPDGELLGIKLESGEQSDPKSKSKKKKSLKKKKDKEKKEKKKGKKSLAKDSASPIQKKKKKKKSAEPAV

Target ORF information:

RefSeq Version	XM_006525777.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus treacle ribosome biogenesis factor 1 (Tcof1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006525777.2

ORF Insert Sequence:

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTG CTTCCCCTCA TTTACCATCA TCTGTTGCAA 
GCAGGCTACG TCCGCGCGGC GCGGGAAGTA AAGGAGCAGA GCGGCCAGAA GAGTTTCCTG 
ACTCAGCCCG TCACCCTTCT GGACATCTAT ACACACTGGC AACAGACCTC AGAGCTTGGC 
CAGAAGCAGA AGGCAGAGGA TGATGAGACC CTTCAGGCTA AGAAGTCTCG AGTGTCGGAT 
CCTGTTAGCA GCTCAGAGAG CTCGGATCAG GAGAAGGAAG AGGAGGCAGC AACCGAAAGG 
GCCAAAGCCA CCCCAAGACC GACACCTGTC AATTCTGCAA CCGCAGCTTT GCCATCAAAA 
GTAAAAGAAA AGGGAAAGAC CAAGACAGCC AACAAGACGG TGAACTCTGT GTCGCACCCT 
GGGTCCGGAA AGACGGTGGT CCACCTGCTC TCTGGGAAGT CACCCAAAAA GTCAGCAGAG 
CCCTTGGCAA ACACTGTCTT GGCCTCAGAA ACTGAGGAGG AGGGCAATGC CCAAGCCCTC 
GGACCCACTG CCAAGTCTGG AACGGTGTCA GCGGGCCAAG GCAGCAGTTC CAGTGAAGAT 
TCCTCCATCT CAAGCGATGA GACAGATGTC GAGGTGAAAT CTCCAGCAAA ACCAGCCCAG 
GCCAAAGCTT CAGCAGCCCC TGCCAAGGAT CCTCCAGCAA GAACAGCCCC AGGCCCTACC 
AAGTTAGGGA ATGTGGCGCC CACACCTGCT AAACCAGCCA GGGCGGCAGC GGCAGCTGCT 
GCTGCTGCTG TGGCTGCTGC TGCTGCTGCA GCAGCAGAAG AGTCTGAGAG CAGTGAGGAG 
GACTCAGACA GTGAGGACGA GGCCCCTGCT GGTCTGCCCA GCCAGGTAAA AGCCTCTGGA 
AAAGGTCCCC ATGTCAGAGC CGACTCGGTG TCTGCCAAGG GGATCTCTGG GAAAGGGCCC 
ATCTTAGCAA CCCCAGGGAA GACTGGGCCT GCAGCCACCC AGGCCAAGGC AGAAAGGCCA 
GAGAAGGACT CGGAAACCAG CAGTGAGGAC GATTCTGATA GTGAGGATGA AATGCCAGTC 
ACTGTGAATA CTCCTCAGGC AAGGACTTCT GGGAAGAGCC CTCGGGCCAG AGGTACCTCA 
GCCCCCGCCA AGGAGTCATC CCAGAAAGGG GCTCCTGCAG TCACCCCTGG AAAGGCAAGG 
CCTGTGGCAG CCCAGGCAGG GAAACCAGAA GCCAAGAGCA GTGAGGAGTC AGAGAGTGAC 
AGTGGGGAGA CACCAGCTGC TGCGACTCTG ACCACGAGTC CTGCCAAGGT GAAACCTTTG 
GGGAAGAGCT CCCAGGTCAG ACCTGTTTCC ACCGTCACCC CGGGGTCATC GGGAAAAGGT 
GCCAACCTGC CCTGCCCTGG GAAGGTGGGG TCAGCAGCTC TCAGGGTCCA AATGGTAAAG 
AAAGAAGATG TCTCGGAGAG CAGCAGTGCA GAGCTGGACA GTGACGGGCC TGGGAGCCCA 
GCCAAGGCAA AGGCCTCCCT TGCGCTCCCT CAGAAGGTGA GGCCTGTGGC CACCCAGGTC 
AAGACTGACA GGGGCAAAGG CCACTCAGGG AGCAGTGAGG AGTCATCTGA CAGCGAAGAG 
GAGGCAGCAC CAGCAGCCTC TGCTGCTCAG GCTAAGCCAG CTCTGGAAAA GCAGATGAAA 
GCTTCCTCTA GGAAAGGCAC GCCTGCATCC GCAACAGGAG CGAGCACCTC GTCCCATTGT 
AAGGCAGGAG CGGTGACCTC TTCAGCCAGC CTGTCATCCC CAGCTCTGGC CAAGGGCACC 
CAGAGGTCAG ATGTGGACTC TTCCAGTGAG TCTGAGTCAG AAGGAGCTGC TCCCAGCACC 
CCCAGGGTAC AGGGGAAGTC TGGGGGCAAG GGCCTCCAAG GGAAAGCTGC CTTGGGGCAA 
GGGGTGGCCC CAGTGCACAC TCAGAAGACA GGGCCTTCGG TCAAAGCTAT GGCTCAGGAA 
GACTCAGAGA GCCTCGAGGA GGACTCCAGC AGTGAGGAAG AGGATGAGAC CCCAGCACAG 
GCCACGCCCT TGGGGAGACT TCCTCAGGCC AAAGCCAACC CACCTCCCAC TAAGACACCT 
CCAGCGTCTG CATCTGGAAA AGCTGTGGCT GCTCCAACCA AGGGAAAACC ACCTGTTCCG 
AACAGCACCG TCTCTGCAAG GGGCCAGCGG TCTGTGCCAG CCGCGGGAAA AGCAGGGGCC 
CCAGCAACCC AAGCCCAGAA GGGTCCCGTG GCTGGCACAG GGGAGGACTC AGAGAGCAGC 
AGTAAAGAGG AGTCTGACAG TGAAGAAGAG ACGCCAGCCC AGATAAAACC TGTGGGGAAG 
ACCTCTCAGG TCAGAGCTGC CTCAGCCCCT GCCAAGGAGT CTCCTAAAAA AGGAGCCCAT 
CCAGGAACCC CCGGCAAGAC GGGATCTTCA GCTACCCAGG CCCAGCCAGG GAAGACAGAG 
GACTCAGACA GCAGTAGTGA AGAGTCTGAC AGTGACACAG AGATGCCATC AGCCCAGGAT 
GCTATCTCCC AGCCTGCCAG AGGCAAAGCG TCAGGTCCAG CGTCCCCAGA GAAGAGCATA 
GAAGGGTCCT CAGAGAGCAG TGATGAGGAT CTGCCCTCCG GCCAGGCGAT TAAATCCCCT 
CCAGTTTCTG TCAACCGTAA CAGTAGTCCA GCTGTCCCAG CTCCTACCCC AGAAGGAGTC 
CAGGCTGTGA ACACCACAAA GAAGGCCTCA GGCACCACTG CCCAGAGCTC CTCCTCTGAG 
AGTGAGGACG GGGACGAGGA CTTGATTCCT GCCACACAAC CCTCCACCTA TGCTCTCAGA 
ACCAGTGTGA CGACGCCCGC AGCCCTCTCA CGAGCAGCTT CCCAACCCAG CAAGAGTGAG 
CAGTCTAGCC GGATGCCAAA AGGCAAGAAA GCAAAGGCGG CGGCGTCCGC TCAGACCAGC 
AGTGCCGTGG AAACACTCCC CATGATGCCT CCCCAGAGCG CACCCATCCA GCCCAAAGCC 
ACCAACAAGC TCGGGAAATC CAAGCTCCCT GAGAAGCAGC AGCTTGCCCC AGGCTACCCC 
AAGGCCCCCA GGAGCTCGGA GGACAGCAGT GACACTTCTT CCGAGGACGA GGAGGATGCC 
AAGAGACCCC AGATGCCCAA GTCGGCCCAC AGGCTGGATC CAGACCCTTC CCAGAAGGAA 
ACTGTGGTAG AGGAGACCCC TACAGAATCC AGCGAAGATG AGATGGTGGC CCCCTCACAG 
TCTCTCCTCT CAGGTTACAT GACTCCCGGC TTGACTGTGG CTAATTCCCA GGCTTCAAAA 
GCTACTCCTA GGCCAGACTC CAACTCCTTA GCTTCTTCTG CCCCGGCCAC CAAAGACAAC 
CCGGATGGCA AGCAGAAGTC AAAATCCCAA CACGCAGCAG ACACCGCACT CCCTAAAACC 
GGTAGGAAAG AGGCCTCCTC GGGCTCCACG CCTCAGAAGC CCAAGAAGCT CAAGAAGAGC 
ACCTCAAGCT CGCCGGCCCC CACACAGACA CTGCCGAACA GCATCACCCA GCGCCTCCTG 
GAGCAGGCCT GGCCCCTGAG CGAGGCGCAG GTGCAGGCCT CTGTGGTGAA GGTCCTGACA 
GAGCTTCTGG AGCAGGAACG GCTGAAGGCC ACAGAGGCCA TCAAGGAGAG TGGGAAGAAG 
AGCCAGAAGC GGAAGTTATC AGGGGACCTA GAAGCTGGGG CCCCAAAGAA CAAGAAGAAG 
AAGGAGCAGC CAGTGCCCAG GGCAAGCGCT GTTTCCCCAG AAAAGGCTCC CATGACTTCC 
AAGGCAAAAT CAAAGCTTGA CAAAGGGAGT GCTGGTGGCA AGGGGAAGGG GTCTCCTGGC 
CCCCAAGGAG CCAAGGAGAA GCCCGACGGC GAGTTGCTGG GGATAAAGCT TGAGAGTGGC 
GAGCAGAGCG ACCCGAAGAG CAAGTCGAAA AAGAAGAAAT CCCTCAAGAA AAAAAAAGAC 
AAGGAGAAAA AGGAAAAGAA GAAAGGAAAA AAGTCCCTGG CCAAAGACTC TGCCTCGCCG 
ATCCAGAAGA AGAAAAAGAA GAAGAAGTCA GCCGAGCCTG CCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tcof1 ( XM_011246874.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011245176.1 Mus musculus treacle ribosome biogenesis factor 1 (Tcof1), transcript variant X2, mRNA.

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CloneID	OMu68855
Clone ID Related Accession (Same CDS sequence)	XM_011246874.2
Accession Version	XM_011246874.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3927bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	treacle protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011246874.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTG CTTCCCCTCA TTTACCATCA TCTGTTGCAA 
GCAGGCTACG TCCGCGCGGC GCGGGAAGTA AAGGAGCAGA GCGGCCAGAA GAGTTTCCTG 
ACTCAGCCCG TCACCCTTCT GGACATCTAT ACACACTGGC AACAGACCTC AGAGCTTGGC 
CAGAAGCAGA AGGCAGAGGA TGATGAGACC CTTCAGGCTA AGAAGTCTCG AGTGTCGGAT 
CCTGTTAGCA GCTCAGAGAG CTCGGATCAG GAGAAGGAAG AGGAGGCAGC AACCGAAAGG 
GCCAAAGCCA CCCCAAGACC GACACCTGTC AATTCTGCAA CCGCAGCTTT GCCATCAAAA 
GTAAAAGAAA AGGGAAAGAC CAAGACAGCC AACAAGACGG TGAACTCTGT GTCGCACCCT 
GGGTCCGGAA AGACGGTGGT CCACCTGCTC TCTGGGAAGT CACCCAAAAA GTCAGCAGAG 
CCCTTGGCAA ACACTGTCTT GGCCTCAGAA ACTGAGGAGG AGGGCAATGC CCAAGCCCTC 
GGACCCACTG CCAAGTCTGG AACGGTGTCA GCGGGCCAAG GCAGCAGTTC CAGTGAAGAT 
TCCTCCATCT CAAGCGATGA GACAGATGTC GAGGTGAAAT CTCCAGCAAA ACCAGCCCAG 
GCCAAAGCTT CAGCAGCCCC TGCCAAGGAT CCTCCAGCAA GAACAGCCCC AGGCCCTACC 
AAGTTAGGGA ATGTGGCGCC CACACCTGCT AAACCAGCCA GGGCGGCAGC GGCAGCTGCT 
GCTGCTGCTG TGGCTGCTGC TGCTGCTGCA GCAGCAGAAG AGTCTGAGAG CAGTGAGGAG 
GACTCAGACA GTGAGGACGA GGCCCCTGCT GGTCTGCCCA GCCAGGTAAA AGCCTCTGGA 
AAAGGTCCCC ATGTCAGAGC CGACTCGGTG TCTGCCAAGG GGATCTCTGG GAAAGGGCCC 
ATCTTAGCAA CCCCAGGGAA GACTGGGCCT GCAGCCACCC AGGCCAAGGC AGAAAGGCCA 
GAGAAGGACT CGGAAACCAG CAGTGAGGAC GATTCTGATA GTGAGGATGA AATGCCAGTC 
ACTGTGAATA CTCCTCAGGC AAGGACTTCT GGGAAGAGCC CTCGGGCCAG AGGTACCTCA 
GCCCCCGCCA AGGAGTCATC CCAGAAAGGG GCTCCTGCAG TCACCCCTGG AAAGGCAAGG 
CCTGTGGCAG CCCAGGCAGG GAAACCAGAA GCCAAGAGCA GTGAGGAGTC AGAGAGTGAC 
AGTGGGGAGA CACCAGCTGC TGCGACTCTG ACCACGAGTC CTGCCAAGGT GAAACCTTTG 
GGGAAGAGCT CCCAGGTCAG ACCTGTTTCC ACCGTCACCC CGGGGTCATC GGGAAAAGGT 
GCCAACCTGC CCTGCCCTGG GAAGGTGGGG TCAGCAGCTC TCAGGGTCCA AATGGTAAAG 
AAAGAAGATG TCTCGGAGAG CAGCAGTGCA GAGCTGGACA GTGACGGGCC TGGGAGCCCA 
GCCAAGGCTA AGCCAGCTCT GGAAAAGCAG ATGAAAGCTT CCTCTAGGAA AGGCACGCCT 
GCATCCGCAA CAGGAGCGAG CACCTCGTCC CATTGTAAGG CAGGAGCGGT GACCTCTTCA 
GCCAGCCTGT CATCCCCAGC TCTGGCCAAG GGCACCCAGA GGTCAGATGT GGACTCTTCC 
AGTGAGTCTG AGTCAGAAGG AGCTGCTCCC AGCACCCCCA GGGTACAGGG GAAGTCTGGG 
GGCAAGGGCC TCCAAGGGAA AGCTGCCTTG GGGCAAGGGG TGGCCCCAGT GCACACTCAG 
AAGACAGGGC CTTCGGTCAA AGCTATGGCT CAGGAAGACT CAGAGAGCCT CGAGGAGGAC 
TCCAGCAGTG AGGAAGAGGA TGAGACCCCA GCACAGGCCA CGCCCTTGGG GAGACTTCCT 
CAGGCCAAAG CCAACCCACC TCCCACTAAG ACACCTCCAG CGTCTGCATC TGGAAAAGCT 
GTGGCTGCTC CAACCAAGGG AAAACCACCT GTTCCGAACA GCACCGTCTC TGCAAGGGGC 
CAGCGGTCTG TGCCAGCCGC GGGAAAAGCA GGGGCCCCAG CAACCCAAGC CCAGAAGGGT 
CCCGTGGCTG GCACAGGGGA GGACTCAGAG AGCAGCAGTA AAGAGGAGTC TGACAGTGAA 
GAAGAGACGC CAGCCCAGAT AAAACCTGTG GGGAAGACCT CTCAGGTCAG AGCTGCCTCA 
GCCCCTGCCA AGGAGTCTCC TAAAAAAGGA GCCCATCCAG GAACCCCCGG CAAGACGGGA 
TCTTCAGCTA CCCAGGCCCA GCCAGGGAAG ACAGAGGACT CAGACAGCAG TAGTGAAGAG 
TCTGACAGTG ACACAGAGAT GCCATCAGCC CAGGATGCTA TCTCCCAGCC TGCCAGAGGC 
AAAGCGTCAG GTCCAGCGTC CCCAGAGAAG AGCATAGAAG GGTCCTCAGA GAGCAGTGAT 
GAGGATCTGC CCTCCGGCCA GGCGATTAAA TCCCCTCCAG TTTCTGTCAA CCGTAACAGT 
AGTCCAGCTG TCCCAGCTCC TACCCCAGAA GGAGTCCAGG CTGTGAACAC CACAAAGAAG 
GCCTCAGGCA CCACTGCCCA GAGCTCCTCC TCTGAGAGTG AGGACGGGGA CGAGGACTTG 
ATTCCTGCCA CACAACCCTC CACCTATGCT CTCAGAACCA GTGTGACGAC GCCCGCAGCC 
CTCTCACGAG CAGCTTCCCA ACCCAGCAAG AGTGAGCAGT CTAGCCGGAT GCCAAAAGGC 
AAGAAAGCAA AGGCGGCGGC GTCCGCTCAG ACCAGCAGTG CCGTGGAAAC ACTCCCCATG 
ATGCCTCCCC AGAGCGCACC CATCCAGCCC AAAGCCACCA ACAAGCTCGG GAAATCCAAG 
CTCCCTGAGA AGCAGCAGCT TGCCCCAGGC TACCCCAAGG CCCCCAGGAG CTCGGAGGAC 
AGCAGTGACA CTTCTTCCGA GGACGAGGAG GATGCCAAGA GACCCCAGAT GCCCAAGTCG 
GCCCACAGGC TGGATCCAGA CCCTTCCCAG AAGGAAACTG TGGTAGAGGA GACCCCTACA 
GAATCCAGCG AAGATGAGAT GGTGGCCCCC TCACAGTCTC TCCTCTCAGG TTACATGACT 
CCCGGCTTGA CTGTGGCTAA TTCCCAGGCT TCAAAAGCTA CTCCTAGGCC AGACTCCAAC 
TCCTTAGCTT CTTCTGCCCC GGCCACCAAA GACAACCCGG ATGGCAAGCA GAAGTCAAAA 
TCCCAACACG CAGCAGACAC CGCACTCCCT AAAACCGGTA GGAAAGAGGC CTCCTCGGGC 
TCCACGCCTC AGAAGCCCAA GAAGCTCAAG AAGAGCACCT CAAGCTCGCC GGCCCCCACA 
CAGACACTGC CGAACAGCAT CACCCAGCGC CTCCTGGAGC AGGCCTGGCC CCTGAGCGAG 
GCGCAGGTGC AGGCCTCTGT GGTGAAGGTC CTGACAGAGC TTCTGGAGCA GGAACGGCTG 
AAGGCCACAG AGGCCATCAA GGAGAGTGGG AAGAAGAGCC AGAAGCGGAA GTTATCAGGG 
GACCTAGAAG CTGGGGCCCC AAAGAACAAG AAGAAGAAGG AGCAGCCAGT GCCCAGGGCA 
AGCGCTGTTT CCCCAGAAAA GGCTCCCATG ACTTCCAAGG CAAAATCAAA GCTTGACAAA 
GGGAGTGCTG GTGGCAAGGG GAAGGGGTCT CCTGGCCCCC AAGGAGCCAA GGAGAAGCCC 
GACGGCGAGT TGCTGGGGAT AAAGCTTGAG AGTGGCGAGC AGAGCGACCC GAAGAGCAAG 
TCGAAAAAGA AGAAATCCCT CAAGAAAAAA AAAGACAAGG AGAAAAAGGA AAAGAAGAAA 
GGAAAAAAGT CCCTGGCCAA AGACTCTGCC TCGCCGATCC AGAAGAAGAA AAAGAAGAAG 
AAGAAGTCAG CCGAGCCTGC CGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011245176.1
CDS	126..4052
Translation	MAEARKRRELLPLIYHHLLQAGYVRAAREVKEQSGQKSFLTQPVTLLDIYTHWQQTSELGQKQKAEDDETLQAKKSRVSDPVSSSESSDQEKEEEAATERAKATPRPTPVNSATAALPSKVKEKGKTKTANKTVNSVSHPGSGKTVVHLLSGKSPKKSAEPLANTVLASETEEEGNAQALGPTAKSGTVSAGQGSSSSEDSSISSDETDVEVKSPAKPAQAKASAAPAKDPPARTAPGPTKLGNVAPTPAKPARAAAAAAAAAVAAAAAAAAEESESSEEDSDSEDEAPAGLPSQVKASGKGPHVRADSVSAKGISGKGPILATPGKTGPAATQAKAERPEKDSETSSEDDSDSEDEMPVTVNTPQARTSGKSPRARGTSAPAKESSQKGAPAVTPGKARPVAAQAGKPEAKSSEESESDSGETPAAATLTTSPAKVKPLGKSSQVRPVSTVTPGSSGKGANLPCPGKVGSAALRVQMVKKEDVSESSSAELDSDGPGSPAKAKPALEKQMKASSRKGTPASATGASTSSHCKAGAVTSSASLSSPALAKGTQRSDVDSSSESESEGAAPSTPRVQGKSGGKGLQGKAALGQGVAPVHTQKTGPSVKAMAQEDSESLEEDSSSEEEDETPAQATPLGRLPQAKANPPPTKTPPASASGKAVAAPTKGKPPVPNSTVSARGQRSVPAAGKAGAPATQAQKGPVAGTGEDSESSSKEESDSEEETPAQIKPVGKTSQVRAASAPAKESPKKGAHPGTPGKTGSSATQAQPGKTEDSDSSSEESDSDTEMPSAQDAISQPARGKASGPASPEKSIEGSSESSDEDLPSGQAIKSPPVSVNRNSSPAVPAPTPEGVQAVNTTKKASGTTAQSSSSESEDGDEDLIPATQPSTYALRTSVTTPAALSRAASQPSKSEQSSRMPKGKKAKAAASAQTSSAVETLPMMPPQSAPIQPKATNKLGKSKLPEKQQLAPGYPKAPRSSEDSSDTSSEDEEDAKRPQMPKSAHRLDPDPSQKETVVEETPTESSEDEMVAPSQSLLSGYMTPGLTVANSQASKATPRPDSNSLASSAPATKDNPDGKQKSKSQHAADTALPKTGRKEASSGSTPQKPKKLKKSTSSSPAPTQTLPNSITQRLLEQAWPLSEAQVQASVVKVLTELLEQERLKATEAIKESGKKSQKRKLSGDLEAGAPKNKKKKEQPVPRASAVSPEKAPMTSKAKSKLDKGSAGGKGKGSPGPQGAKEKPDGELLGIKLESGEQSDPKSKSKKKKSLKKKKDKEKKEKKKGKKSLAKDSASPIQKKKKKKKKSAEPAV

Target ORF information:

RefSeq Version	XM_011246874.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus treacle ribosome biogenesis factor 1 (Tcof1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011246874.2

ORF Insert Sequence:

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTG CTTCCCCTCA TTTACCATCA TCTGTTGCAA 
GCAGGCTACG TCCGCGCGGC GCGGGAAGTA AAGGAGCAGA GCGGCCAGAA GAGTTTCCTG 
ACTCAGCCCG TCACCCTTCT GGACATCTAT ACACACTGGC AACAGACCTC AGAGCTTGGC 
CAGAAGCAGA AGGCAGAGGA TGATGAGACC CTTCAGGCTA AGAAGTCTCG AGTGTCGGAT 
CCTGTTAGCA GCTCAGAGAG CTCGGATCAG GAGAAGGAAG AGGAGGCAGC AACCGAAAGG 
GCCAAAGCCA CCCCAAGACC GACACCTGTC AATTCTGCAA CCGCAGCTTT GCCATCAAAA 
GTAAAAGAAA AGGGAAAGAC CAAGACAGCC AACAAGACGG TGAACTCTGT GTCGCACCCT 
GGGTCCGGAA AGACGGTGGT CCACCTGCTC TCTGGGAAGT CACCCAAAAA GTCAGCAGAG 
CCCTTGGCAA ACACTGTCTT GGCCTCAGAA ACTGAGGAGG AGGGCAATGC CCAAGCCCTC 
GGACCCACTG CCAAGTCTGG AACGGTGTCA GCGGGCCAAG GCAGCAGTTC CAGTGAAGAT 
TCCTCCATCT CAAGCGATGA GACAGATGTC GAGGTGAAAT CTCCAGCAAA ACCAGCCCAG 
GCCAAAGCTT CAGCAGCCCC TGCCAAGGAT CCTCCAGCAA GAACAGCCCC AGGCCCTACC 
AAGTTAGGGA ATGTGGCGCC CACACCTGCT AAACCAGCCA GGGCGGCAGC GGCAGCTGCT 
GCTGCTGCTG TGGCTGCTGC TGCTGCTGCA GCAGCAGAAG AGTCTGAGAG CAGTGAGGAG 
GACTCAGACA GTGAGGACGA GGCCCCTGCT GGTCTGCCCA GCCAGGTAAA AGCCTCTGGA 
AAAGGTCCCC ATGTCAGAGC CGACTCGGTG TCTGCCAAGG GGATCTCTGG GAAAGGGCCC 
ATCTTAGCAA CCCCAGGGAA GACTGGGCCT GCAGCCACCC AGGCCAAGGC AGAAAGGCCA 
GAGAAGGACT CGGAAACCAG CAGTGAGGAC GATTCTGATA GTGAGGATGA AATGCCAGTC 
ACTGTGAATA CTCCTCAGGC AAGGACTTCT GGGAAGAGCC CTCGGGCCAG AGGTACCTCA 
GCCCCCGCCA AGGAGTCATC CCAGAAAGGG GCTCCTGCAG TCACCCCTGG AAAGGCAAGG 
CCTGTGGCAG CCCAGGCAGG GAAACCAGAA GCCAAGAGCA GTGAGGAGTC AGAGAGTGAC 
AGTGGGGAGA CACCAGCTGC TGCGACTCTG ACCACGAGTC CTGCCAAGGT GAAACCTTTG 
GGGAAGAGCT CCCAGGTCAG ACCTGTTTCC ACCGTCACCC CGGGGTCATC GGGAAAAGGT 
GCCAACCTGC CCTGCCCTGG GAAGGTGGGG TCAGCAGCTC TCAGGGTCCA AATGGTAAAG 
AAAGAAGATG TCTCGGAGAG CAGCAGTGCA GAGCTGGACA GTGACGGGCC TGGGAGCCCA 
GCCAAGGCTA AGCCAGCTCT GGAAAAGCAG ATGAAAGCTT CCTCTAGGAA AGGCACGCCT 
GCATCCGCAA CAGGAGCGAG CACCTCGTCC CATTGTAAGG CAGGAGCGGT GACCTCTTCA 
GCCAGCCTGT CATCCCCAGC TCTGGCCAAG GGCACCCAGA GGTCAGATGT GGACTCTTCC 
AGTGAGTCTG AGTCAGAAGG AGCTGCTCCC AGCACCCCCA GGGTACAGGG GAAGTCTGGG 
GGCAAGGGCC TCCAAGGGAA AGCTGCCTTG GGGCAAGGGG TGGCCCCAGT GCACACTCAG 
AAGACAGGGC CTTCGGTCAA AGCTATGGCT CAGGAAGACT CAGAGAGCCT CGAGGAGGAC 
TCCAGCAGTG AGGAAGAGGA TGAGACCCCA GCACAGGCCA CGCCCTTGGG GAGACTTCCT 
CAGGCCAAAG CCAACCCACC TCCCACTAAG ACACCTCCAG CGTCTGCATC TGGAAAAGCT 
GTGGCTGCTC CAACCAAGGG AAAACCACCT GTTCCGAACA GCACCGTCTC TGCAAGGGGC 
CAGCGGTCTG TGCCAGCCGC GGGAAAAGCA GGGGCCCCAG CAACCCAAGC CCAGAAGGGT 
CCCGTGGCTG GCACAGGGGA GGACTCAGAG AGCAGCAGTA AAGAGGAGTC TGACAGTGAA 
GAAGAGACGC CAGCCCAGAT AAAACCTGTG GGGAAGACCT CTCAGGTCAG AGCTGCCTCA 
GCCCCTGCCA AGGAGTCTCC TAAAAAAGGA GCCCATCCAG GAACCCCCGG CAAGACGGGA 
TCTTCAGCTA CCCAGGCCCA GCCAGGGAAG ACAGAGGACT CAGACAGCAG TAGTGAAGAG 
TCTGACAGTG ACACAGAGAT GCCATCAGCC CAGGATGCTA TCTCCCAGCC TGCCAGAGGC 
AAAGCGTCAG GTCCAGCGTC CCCAGAGAAG AGCATAGAAG GGTCCTCAGA GAGCAGTGAT 
GAGGATCTGC CCTCCGGCCA GGCGATTAAA TCCCCTCCAG TTTCTGTCAA CCGTAACAGT 
AGTCCAGCTG TCCCAGCTCC TACCCCAGAA GGAGTCCAGG CTGTGAACAC CACAAAGAAG 
GCCTCAGGCA CCACTGCCCA GAGCTCCTCC TCTGAGAGTG AGGACGGGGA CGAGGACTTG 
ATTCCTGCCA CACAACCCTC CACCTATGCT CTCAGAACCA GTGTGACGAC GCCCGCAGCC 
CTCTCACGAG CAGCTTCCCA ACCCAGCAAG AGTGAGCAGT CTAGCCGGAT GCCAAAAGGC 
AAGAAAGCAA AGGCGGCGGC GTCCGCTCAG ACCAGCAGTG CCGTGGAAAC ACTCCCCATG 
ATGCCTCCCC AGAGCGCACC CATCCAGCCC AAAGCCACCA ACAAGCTCGG GAAATCCAAG 
CTCCCTGAGA AGCAGCAGCT TGCCCCAGGC TACCCCAAGG CCCCCAGGAG CTCGGAGGAC 
AGCAGTGACA CTTCTTCCGA GGACGAGGAG GATGCCAAGA GACCCCAGAT GCCCAAGTCG 
GCCCACAGGC TGGATCCAGA CCCTTCCCAG AAGGAAACTG TGGTAGAGGA GACCCCTACA 
GAATCCAGCG AAGATGAGAT GGTGGCCCCC TCACAGTCTC TCCTCTCAGG TTACATGACT 
CCCGGCTTGA CTGTGGCTAA TTCCCAGGCT TCAAAAGCTA CTCCTAGGCC AGACTCCAAC 
TCCTTAGCTT CTTCTGCCCC GGCCACCAAA GACAACCCGG ATGGCAAGCA GAAGTCAAAA 
TCCCAACACG CAGCAGACAC CGCACTCCCT AAAACCGGTA GGAAAGAGGC CTCCTCGGGC 
TCCACGCCTC AGAAGCCCAA GAAGCTCAAG AAGAGCACCT CAAGCTCGCC GGCCCCCACA 
CAGACACTGC CGAACAGCAT CACCCAGCGC CTCCTGGAGC AGGCCTGGCC CCTGAGCGAG 
GCGCAGGTGC AGGCCTCTGT GGTGAAGGTC CTGACAGAGC TTCTGGAGCA GGAACGGCTG 
AAGGCCACAG AGGCCATCAA GGAGAGTGGG AAGAAGAGCC AGAAGCGGAA GTTATCAGGG 
GACCTAGAAG CTGGGGCCCC AAAGAACAAG AAGAAGAAGG AGCAGCCAGT GCCCAGGGCA 
AGCGCTGTTT CCCCAGAAAA GGCTCCCATG ACTTCCAAGG CAAAATCAAA GCTTGACAAA 
GGGAGTGCTG GTGGCAAGGG GAAGGGGTCT CCTGGCCCCC AAGGAGCCAA GGAGAAGCCC 
GACGGCGAGT TGCTGGGGAT AAAGCTTGAG AGTGGCGAGC AGAGCGACCC GAAGAGCAAG 
TCGAAAAAGA AGAAATCCCT CAAGAAAAAA AAAGACAAGG AGAAAAAGGA AAAGAAGAAA 
GGAAAAAAGT CCCTGGCCAA AGACTCTGCC TCGCCGATCC AGAAGAAGAA AAAGAAGAAG 
AAGAAGTCAG CCGAGCCTGC CGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tcof1 ( XM_011246875.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011245177.1 Mus musculus treacle ribosome biogenesis factor 1 (Tcof1), transcript variant X3, mRNA.

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CloneID	OMu68856
Clone ID Related Accession (Same CDS sequence)	XM_011246875.2
Accession Version	XM_011246875.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3819bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	treacle protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011246875.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTG CTTCCCCTCA TTTACCATCA TCTGTTGCAA 
GCAGGCTACG TCCGCGCGGC GCGGGAAGTA AAGGAGCAGA GCGGCCAGAA GAGTTTCCTG 
ACTCAGCCCG TCACCCTTCT GGACATCTAT ACACACTGGC AACAGACCTC AGAGCTTGGC 
CAGAAGCAGA AGGCAGAGGA TGATGAGACC CTTCAGGCTA AGAAGTCTCG AGTGTCGGAT 
CCTGTTAGCA GCTCAGAGAG CTCGGATCAG GAGAAGGAAG AGGAGGCAGC AACCGAAAGG 
GCCAAAGCCA CCCCAAGACC GACACCTGTC AATTCTGCAA CCGCAGCTTT GCCATCAAAA 
GTAAAAGAAA AGGGAAAGAC CAAGACAGCC AACAAGACGG TGAACTCTGT GTCGCACCCT 
GGGTCCGGAA AGACGGTGGT CCACCTGCTC TCTGGGAAGT CACCCAAAAA GTCAGCAGAG 
CCCTTGGCAA ACACTGTCTT GGCCTCAGAA ACTGAGGAGG AGGGCAATGC CCAAGCCCTC 
GGACCCACTG CCAAGTCTGG AACGGTGTCA GCGGGCCAAG GCAGCAGTTC CAGTGAAGAT 
TCCTCCATCT CAAGCGATGA GACAGATGTC GAGGTGAAAT CTCCAGCAAA ACCAGCCCAG 
GCCAAAGCTT CAGCAGCCCC TGCCAAGGAT CCTCCAGCAA GAACAGCCCC AGGCCCTACC 
AAGTTAGGGA ATGTGGCGCC CACACCTGCT AAACCAGCCA GGGCGGCAGC GGCAGCTGCT 
GCTGCTGCTG TGGCTGCTGC TGCTGCTGCA GCAGCAGAAG AGTCTGAGAG CAGTGAGGAG 
GACTCAGACA GTGAGGACGA GGCCCCTGCT GGTCTGCCCA GCCAGGTAAA AGCCTCTGGA 
AAAGGTCCCC ATGTCAGAGC CGACTCGGTG TCTGCCAAGG GGATCTCTGG GAAAGGGCCC 
ATCTTAGCAA CCCCAGGGAA GACTGGGCCT GCAGCCACCC AGGCCAAGGC AGAAAGGCCA 
GAGAAGGACT CGGAAACCAG CAGTGAGGAC GATTCTGATA GTGAGGATGA AATGCCAGTC 
ACTGTGAATA CTCCTCAGGC AAGGACTTCT GGGAAGAGCC CTCGGGCCAG AGGTACCTCA 
GCCCCCGCCA AGGAGTCATC CCAGAAAGGG GCTCCTGCAG TCACCCCTGG AAAGGCAAGG 
CCTGTGGCAG CCCAGGCAGG GAAACCAGAA GCCAAGAGCA GTGAGGAGTC AGAGAGTGAC 
AGTGGGGAGA CACCAGCTGC TGCGACTCTG ACCACGAGTC CTGCCAAGGT GAAACCTTTG 
GGGAAGAGCT CCCAGGTCAG ACCTGTTTCC ACCGTCACCC CGGGGTCATC GGGAAAAGGT 
GCCAACCTGC CCTGCCCTGG GAAGGTGGGG TCAGCAGCTC TCAGGGTCCA AATGGTAAAG 
AAAGAAGATG TCTCGGAGAG CAGCAGTGCA GAGCTGGACA GTGACGGGCC TGGGAGCCCA 
GCCAAGGCTA AGCCAGCTCT GGAAAAGCAG ATGAAAGCTT CCTCTAGGAA AGGCACGCCT 
GCATCCGCAA CAGGAGCGAG CACCTCGTCC CATTGTAAGG CAGGAGCGGT GACCTCTTCA 
GCCAGCCTGT CATCCCCAGC TCTGGCCAAG GGCACCCAGA GGTCAGATGT GGACTCTTCC 
AGTGAGTCTG AGTCAGAAGG AGCTGCTCCC AGCACCCCCA GGGTACAGGG GAAGTCTGGG 
GGCAAGGGCC TCCAAGGGAA AGCTGCCTTG GGGCAAGGGG TGGCCCCAGT GCACACTCAG 
AAGACAGGGC CTTCGGTCAA AGCTATGGCT CAGGAAGACT CAGAGAGCCT CGAGGAGGAC 
TCCAGCAGTG AGGAAGAGGA TGAGACCCCA GCACAGGCCA CGCCCTTGGG GAGACTTCCT 
CAGGCCAAAG CCAACCCACC TCCCACTAAG ACACCTCCAG CGTCTGCATC TGGAAAAGCT 
GTGGCTGCTC CAACCAAGGG AAAACCACCT GTTCCGAACA GCACCGTCTC TGCAAGGGGC 
CAGCGGTCTG TGCCAGCCGC GGGAAAAGCA GGGGCCCCAG CAACCCAAGC CCAGAAGGGT 
CCCGTGGCTG GCACAGGGGA GGACTCAGAG AGCAGCAGTA AAGAGGAGTC TGACAGTGAA 
GAAGAGACGC CAGCCCAGAT AAAACCTGTG GGGAAGACCT CTCAGGTCAG AGCTGCCTCA 
GCCCCTGCCA AGGAGTCTCC TAAAAAAGGA GCCCATCCAG GAACCCCCGG CAAGACGGGA 
TCTTCAGCTA CCCAGGCCCA GCCAGGGAAG ACAGAGGACT CAGACAGCAG TAGTGAAGAG 
TCTGACAGTG ACACAGAGAT GCCATCAGCC CAGGCGATTA AATCCCCTCC AGTTTCTGTC 
AACCGTAACA GTAGTCCAGC TGTCCCAGCT CCTACCCCAG AAGGAGTCCA GGCTGTGAAC 
ACCACAAAGA AGGCCTCAGG CACCACTGCC CAGAGCTCCT CCTCTGAGAG TGAGGACGGG 
GACGAGGACT TGATTCCTGC CACACAACCC TCCACCTATG CTCTCAGAAC CAGTGTGACG 
ACGCCCGCAG CCCTCTCACG AGCAGCTTCC CAACCCAGCA AGAGTGAGCA GTCTAGCCGG 
ATGCCAAAAG GCAAGAAAGC AAAGGCGGCG GCGTCCGCTC AGACCAGCAG TGCCGTGGAA 
ACACTCCCCA TGATGCCTCC CCAGAGCGCA CCCATCCAGC CCAAAGCCAC CAACAAGCTC 
GGGAAATCCA AGCTCCCTGA GAAGCAGCAG CTTGCCCCAG GCTACCCCAA GGCCCCCAGG 
AGCTCGGAGG ACAGCAGTGA CACTTCTTCC GAGGACGAGG AGGATGCCAA GAGACCCCAG 
ATGCCCAAGT CGGCCCACAG GCTGGATCCA GACCCTTCCC AGAAGGAAAC TGTGGTAGAG 
GAGACCCCTA CAGAATCCAG CGAAGATGAG ATGGTGGCCC CCTCACAGTC TCTCCTCTCA 
GGTTACATGA CTCCCGGCTT GACTGTGGCT AATTCCCAGG CTTCAAAAGC TACTCCTAGG 
CCAGACTCCA ACTCCTTAGC TTCTTCTGCC CCGGCCACCA AAGACAACCC GGATGGCAAG 
CAGAAGTCAA AATCCCAACA CGCAGCAGAC ACCGCACTCC CTAAAACCGG TAGGAAAGAG 
GCCTCCTCGG GCTCCACGCC TCAGAAGCCC AAGAAGCTCA AGAAGAGCAC CTCAAGCTCG 
CCGGCCCCCA CACAGACACT GCCGAACAGC ATCACCCAGC GCCTCCTGGA GCAGGCCTGG 
CCCCTGAGCG AGGCGCAGGT GCAGGCCTCT GTGGTGAAGG TCCTGACAGA GCTTCTGGAG 
CAGGAACGGC TGAAGGCCAC AGAGGCCATC AAGGAGAGTG GGAAGAAGAG CCAGAAGCGG 
AAGTTATCAG GGGACCTAGA AGCTGGGGCC CCAAAGAACA AGAAGAAGAA GGAGCAGCCA 
GTGCCCAGGG CAAGCGCTGT TTCCCCAGAA AAGGCTCCCA TGACTTCCAA GGCAAAATCA 
AAGCTTGACA AAGGGAGTGC TGGTGGCAAG GGGAAGGGGT CTCCTGGCCC CCAAGGAGCC 
AAGGAGAAGC CCGACGGCGA GTTGCTGGGG ATAAAGCTTG AGAGTGGCGA GCAGAGCGAC 
CCGAAGAGCA AGTCGAAAAA GAAGAAATCC CTCAAGAAAA AAAAAGACAA GGAGAAAAAG 
GAAAAGAAGA AAGGAAAAAA GTCCCTGGCC AAAGACTCTG CCTCGCCGAT CCAGAAGAAG 
AAAAAGAAGA AGAAGAAGTC AGCCGAGCCT GCCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011245177.1
CDS	126..3944
Translation	MAEARKRRELLPLIYHHLLQAGYVRAAREVKEQSGQKSFLTQPVTLLDIYTHWQQTSELGQKQKAEDDETLQAKKSRVSDPVSSSESSDQEKEEEAATERAKATPRPTPVNSATAALPSKVKEKGKTKTANKTVNSVSHPGSGKTVVHLLSGKSPKKSAEPLANTVLASETEEEGNAQALGPTAKSGTVSAGQGSSSSEDSSISSDETDVEVKSPAKPAQAKASAAPAKDPPARTAPGPTKLGNVAPTPAKPARAAAAAAAAAVAAAAAAAAEESESSEEDSDSEDEAPAGLPSQVKASGKGPHVRADSVSAKGISGKGPILATPGKTGPAATQAKAERPEKDSETSSEDDSDSEDEMPVTVNTPQARTSGKSPRARGTSAPAKESSQKGAPAVTPGKARPVAAQAGKPEAKSSEESESDSGETPAAATLTTSPAKVKPLGKSSQVRPVSTVTPGSSGKGANLPCPGKVGSAALRVQMVKKEDVSESSSAELDSDGPGSPAKAKPALEKQMKASSRKGTPASATGASTSSHCKAGAVTSSASLSSPALAKGTQRSDVDSSSESESEGAAPSTPRVQGKSGGKGLQGKAALGQGVAPVHTQKTGPSVKAMAQEDSESLEEDSSSEEEDETPAQATPLGRLPQAKANPPPTKTPPASASGKAVAAPTKGKPPVPNSTVSARGQRSVPAAGKAGAPATQAQKGPVAGTGEDSESSSKEESDSEEETPAQIKPVGKTSQVRAASAPAKESPKKGAHPGTPGKTGSSATQAQPGKTEDSDSSSEESDSDTEMPSAQAIKSPPVSVNRNSSPAVPAPTPEGVQAVNTTKKASGTTAQSSSSESEDGDEDLIPATQPSTYALRTSVTTPAALSRAASQPSKSEQSSRMPKGKKAKAAASAQTSSAVETLPMMPPQSAPIQPKATNKLGKSKLPEKQQLAPGYPKAPRSSEDSSDTSSEDEEDAKRPQMPKSAHRLDPDPSQKETVVEETPTESSEDEMVAPSQSLLSGYMTPGLTVANSQASKATPRPDSNSLASSAPATKDNPDGKQKSKSQHAADTALPKTGRKEASSGSTPQKPKKLKKSTSSSPAPTQTLPNSITQRLLEQAWPLSEAQVQASVVKVLTELLEQERLKATEAIKESGKKSQKRKLSGDLEAGAPKNKKKKEQPVPRASAVSPEKAPMTSKAKSKLDKGSAGGKGKGSPGPQGAKEKPDGELLGIKLESGEQSDPKSKSKKKKSLKKKKDKEKKEKKKGKKSLAKDSASPIQKKKKKKKKSAEPAV

Target ORF information:

RefSeq Version	XM_011246875.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus treacle ribosome biogenesis factor 1 (Tcof1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011246875.2

ORF Insert Sequence:

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTG CTTCCCCTCA TTTACCATCA TCTGTTGCAA 
GCAGGCTACG TCCGCGCGGC GCGGGAAGTA AAGGAGCAGA GCGGCCAGAA GAGTTTCCTG 
ACTCAGCCCG TCACCCTTCT GGACATCTAT ACACACTGGC AACAGACCTC AGAGCTTGGC 
CAGAAGCAGA AGGCAGAGGA TGATGAGACC CTTCAGGCTA AGAAGTCTCG AGTGTCGGAT 
CCTGTTAGCA GCTCAGAGAG CTCGGATCAG GAGAAGGAAG AGGAGGCAGC AACCGAAAGG 
GCCAAAGCCA CCCCAAGACC GACACCTGTC AATTCTGCAA CCGCAGCTTT GCCATCAAAA 
GTAAAAGAAA AGGGAAAGAC CAAGACAGCC AACAAGACGG TGAACTCTGT GTCGCACCCT 
GGGTCCGGAA AGACGGTGGT CCACCTGCTC TCTGGGAAGT CACCCAAAAA GTCAGCAGAG 
CCCTTGGCAA ACACTGTCTT GGCCTCAGAA ACTGAGGAGG AGGGCAATGC CCAAGCCCTC 
GGACCCACTG CCAAGTCTGG AACGGTGTCA GCGGGCCAAG GCAGCAGTTC CAGTGAAGAT 
TCCTCCATCT CAAGCGATGA GACAGATGTC GAGGTGAAAT CTCCAGCAAA ACCAGCCCAG 
GCCAAAGCTT CAGCAGCCCC TGCCAAGGAT CCTCCAGCAA GAACAGCCCC AGGCCCTACC 
AAGTTAGGGA ATGTGGCGCC CACACCTGCT AAACCAGCCA GGGCGGCAGC GGCAGCTGCT 
GCTGCTGCTG TGGCTGCTGC TGCTGCTGCA GCAGCAGAAG AGTCTGAGAG CAGTGAGGAG 
GACTCAGACA GTGAGGACGA GGCCCCTGCT GGTCTGCCCA GCCAGGTAAA AGCCTCTGGA 
AAAGGTCCCC ATGTCAGAGC CGACTCGGTG TCTGCCAAGG GGATCTCTGG GAAAGGGCCC 
ATCTTAGCAA CCCCAGGGAA GACTGGGCCT GCAGCCACCC AGGCCAAGGC AGAAAGGCCA 
GAGAAGGACT CGGAAACCAG CAGTGAGGAC GATTCTGATA GTGAGGATGA AATGCCAGTC 
ACTGTGAATA CTCCTCAGGC AAGGACTTCT GGGAAGAGCC CTCGGGCCAG AGGTACCTCA 
GCCCCCGCCA AGGAGTCATC CCAGAAAGGG GCTCCTGCAG TCACCCCTGG AAAGGCAAGG 
CCTGTGGCAG CCCAGGCAGG GAAACCAGAA GCCAAGAGCA GTGAGGAGTC AGAGAGTGAC 
AGTGGGGAGA CACCAGCTGC TGCGACTCTG ACCACGAGTC CTGCCAAGGT GAAACCTTTG 
GGGAAGAGCT CCCAGGTCAG ACCTGTTTCC ACCGTCACCC CGGGGTCATC GGGAAAAGGT 
GCCAACCTGC CCTGCCCTGG GAAGGTGGGG TCAGCAGCTC TCAGGGTCCA AATGGTAAAG 
AAAGAAGATG TCTCGGAGAG CAGCAGTGCA GAGCTGGACA GTGACGGGCC TGGGAGCCCA 
GCCAAGGCTA AGCCAGCTCT GGAAAAGCAG ATGAAAGCTT CCTCTAGGAA AGGCACGCCT 
GCATCCGCAA CAGGAGCGAG CACCTCGTCC CATTGTAAGG CAGGAGCGGT GACCTCTTCA 
GCCAGCCTGT CATCCCCAGC TCTGGCCAAG GGCACCCAGA GGTCAGATGT GGACTCTTCC 
AGTGAGTCTG AGTCAGAAGG AGCTGCTCCC AGCACCCCCA GGGTACAGGG GAAGTCTGGG 
GGCAAGGGCC TCCAAGGGAA AGCTGCCTTG GGGCAAGGGG TGGCCCCAGT GCACACTCAG 
AAGACAGGGC CTTCGGTCAA AGCTATGGCT CAGGAAGACT CAGAGAGCCT CGAGGAGGAC 
TCCAGCAGTG AGGAAGAGGA TGAGACCCCA GCACAGGCCA CGCCCTTGGG GAGACTTCCT 
CAGGCCAAAG CCAACCCACC TCCCACTAAG ACACCTCCAG CGTCTGCATC TGGAAAAGCT 
GTGGCTGCTC CAACCAAGGG AAAACCACCT GTTCCGAACA GCACCGTCTC TGCAAGGGGC 
CAGCGGTCTG TGCCAGCCGC GGGAAAAGCA GGGGCCCCAG CAACCCAAGC CCAGAAGGGT 
CCCGTGGCTG GCACAGGGGA GGACTCAGAG AGCAGCAGTA AAGAGGAGTC TGACAGTGAA 
GAAGAGACGC CAGCCCAGAT AAAACCTGTG GGGAAGACCT CTCAGGTCAG AGCTGCCTCA 
GCCCCTGCCA AGGAGTCTCC TAAAAAAGGA GCCCATCCAG GAACCCCCGG CAAGACGGGA 
TCTTCAGCTA CCCAGGCCCA GCCAGGGAAG ACAGAGGACT CAGACAGCAG TAGTGAAGAG 
TCTGACAGTG ACACAGAGAT GCCATCAGCC CAGGCGATTA AATCCCCTCC AGTTTCTGTC 
AACCGTAACA GTAGTCCAGC TGTCCCAGCT CCTACCCCAG AAGGAGTCCA GGCTGTGAAC 
ACCACAAAGA AGGCCTCAGG CACCACTGCC CAGAGCTCCT CCTCTGAGAG TGAGGACGGG 
GACGAGGACT TGATTCCTGC CACACAACCC TCCACCTATG CTCTCAGAAC CAGTGTGACG 
ACGCCCGCAG CCCTCTCACG AGCAGCTTCC CAACCCAGCA AGAGTGAGCA GTCTAGCCGG 
ATGCCAAAAG GCAAGAAAGC AAAGGCGGCG GCGTCCGCTC AGACCAGCAG TGCCGTGGAA 
ACACTCCCCA TGATGCCTCC CCAGAGCGCA CCCATCCAGC CCAAAGCCAC CAACAAGCTC 
GGGAAATCCA AGCTCCCTGA GAAGCAGCAG CTTGCCCCAG GCTACCCCAA GGCCCCCAGG 
AGCTCGGAGG ACAGCAGTGA CACTTCTTCC GAGGACGAGG AGGATGCCAA GAGACCCCAG 
ATGCCCAAGT CGGCCCACAG GCTGGATCCA GACCCTTCCC AGAAGGAAAC TGTGGTAGAG 
GAGACCCCTA CAGAATCCAG CGAAGATGAG ATGGTGGCCC CCTCACAGTC TCTCCTCTCA 
GGTTACATGA CTCCCGGCTT GACTGTGGCT AATTCCCAGG CTTCAAAAGC TACTCCTAGG 
CCAGACTCCA ACTCCTTAGC TTCTTCTGCC CCGGCCACCA AAGACAACCC GGATGGCAAG 
CAGAAGTCAA AATCCCAACA CGCAGCAGAC ACCGCACTCC CTAAAACCGG TAGGAAAGAG 
GCCTCCTCGG GCTCCACGCC TCAGAAGCCC AAGAAGCTCA AGAAGAGCAC CTCAAGCTCG 
CCGGCCCCCA CACAGACACT GCCGAACAGC ATCACCCAGC GCCTCCTGGA GCAGGCCTGG 
CCCCTGAGCG AGGCGCAGGT GCAGGCCTCT GTGGTGAAGG TCCTGACAGA GCTTCTGGAG 
CAGGAACGGC TGAAGGCCAC AGAGGCCATC AAGGAGAGTG GGAAGAAGAG CCAGAAGCGG 
AAGTTATCAG GGGACCTAGA AGCTGGGGCC CCAAAGAACA AGAAGAAGAA GGAGCAGCCA 
GTGCCCAGGG CAAGCGCTGT TTCCCCAGAA AAGGCTCCCA TGACTTCCAA GGCAAAATCA 
AAGCTTGACA AAGGGAGTGC TGGTGGCAAG GGGAAGGGGT CTCCTGGCCC CCAAGGAGCC 
AAGGAGAAGC CCGACGGCGA GTTGCTGGGG ATAAAGCTTG AGAGTGGCGA GCAGAGCGAC 
CCGAAGAGCA AGTCGAAAAA GAAGAAATCC CTCAAGAAAA AAAAAGACAA GGAGAAAAAG 
GAAAAGAAGA AAGGAAAAAA GTCCCTGGCC AAAGACTCTG CCTCGCCGAT CCAGAAGAAG 
AAAAAGAAGA AGAAGAAGTC AGCCGAGCCT GCCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Face off against ROS: Tcof1/Treacle safeguards neuroepithelial cells and progenitor neural crest cells from oxidative stress during craniofacial development.
Development, growth & differentiation58(7)577-85(2016 Sep)
Sakai D,Trainor PA

A quantitative method for defining high-arched palate using the Tcof1(+/-) mutant mouse as a model.
Developmental biology415(2)296-305(2016 07)
Conley ZR,Hague M,Kurosaka H,Dixon J,Dixon MJ,Trainor PA

Prevention of Treacher Collins syndrome craniofacial anomalies in mouse models via maternal antioxidant supplementation.
Nature communications710328(2016 Jan)
Sakai D,Dixon J,Achilleos A,Dixon M,Trainor PA

Quantitative trait loci affecting the 3D skull shape and size in mouse and prioritization of candidate genes in-silico.
Frontiers in physiology692(2015)
Maga AM,Navarro N,Cunningham ML,Cox TC

Resistance to noise-induced hearing loss in 129S6 and MOLF mice: identification of independent, overlapping, and interacting chromosomal regions.
Journal of the Association for Research in Otolaryngology : JARO15(5)721-38(2014 Oct)
Street VA,Kujawa SG,Manichaikul A,Broman KW,Kallman JC,Shilling DJ,Iwata AJ,Robinson LC,Robbins CA,Li J,Liberman MC,Tempel BL

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Tcof1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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