TCOF1 gene cDNA ORF clone, Pan troglodytes(chimpanzee)

mRNA	Protein	Name
XM_016954040.2	XP_016809529.2	treacle protein isoform X1
XM_016954040.1	XP_016809529.1	treacle protein isoform X1
XM_016954043.1	XP_016809532.1	treacle protein isoform X4
XM_016954042.2	XP_016809531.2	treacle protein isoform X3
XM_016954043.2	XP_016809532.2	treacle protein isoform X4
XM_016954044.1	XP_016809533.1	treacle protein isoform X5
XM_016954047.1	XP_016809536.1	treacle protein isoform X7
XM_016954041.2	XP_016809530.2	treacle protein isoform X2
XM_016954044.2	XP_016809533.2	treacle protein isoform X5
XM_016954041.1	XP_016809530.1	treacle protein isoform X2
XM_016954042.1	XP_016809531.1	treacle protein isoform X3
XM_016954046.1	XP_016809535.1	treacle protein isoform X6
XM_016954048.1	XP_016809537.1	treacle protein isoform X8
XM_016954048.2	XP_016809537.2	treacle protein isoform X8
XM_016954046.2	XP_016809535.2	treacle protein isoform X6
XM_016954047.2	XP_016809536.2	treacle protein isoform X7

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPc106680	XM_016954048.2 Latest version!	Pan troglodytes treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OPc106675	XM_016954042.2 Latest version!	Pan troglodytes treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OPc106676	XM_016954043.2 Latest version!	Pan troglodytes treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OPc106677	XM_016954044.2 Latest version!	Pan troglodytes treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OPc106678	XM_016954046.2 Latest version!	Pan troglodytes treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OPc106679	XM_016954047.2 Latest version!	Pan troglodytes treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OPc106673	XM_016954040.2 Latest version!	Pan troglodytes treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OPc106674	XM_016954041.2 Latest version!	Pan troglodytes treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OPc85507	XM_016954040.1 Latest version!	Pan troglodytes treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OPc85508	XM_016954041.1 Latest version!	Pan troglodytes treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OPc85509	XM_016954042.1 Latest version!	Pan troglodytes treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OPc85510	XM_016954043.1 Latest version!	Pan troglodytes treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OPc85511	XM_016954044.1 Latest version!	Pan troglodytes treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OPc85512	XM_016954046.1 Latest version!	Pan troglodytes treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OPc85513	XM_016954047.1 Latest version!	Pan troglodytes treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OPc85514	XM_016954048.1 Latest version!	Pan troglodytes treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00

TCOF1 ( XM_016954048.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016809537.2 Pan troglodytes treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OPc106680
Clone ID Related Accession (Same CDS sequence)	XM_016954048.2
Accession Version	XM_016954048.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2877bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	treacle protein isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036884.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016954048.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCAGAGAG CTCGGAAGAG GAGGAGGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCGAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCTTG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGT 
GAGGACACCT CCAGCTCCAG CGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCGCCAGCC 
AAGAAGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CGGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GAGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGTG AGGAGTCGTC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GTGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAAGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG ACTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAGGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCC ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCTGG GAAGGTGGGG CCTGCAACCC CCTCGGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCT 
GCCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CGTCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGTCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCGGC CTCAGTGCCT CTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAGTCAGAC AGTGAGGAGG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGTCAAA 
CAGAGATCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCGGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GTGGAGACGC TGGCTCAGGC GAAGCCTTCA GGGAAGACCC CCCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AAGCACCGGC AGCTGTGACC TCTGCCCAGG ACCAGGAGTC TTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016809537.2
CDS	104..2980
Translation	MAEARKRRELLPLIYHHLLRAGYVRAAREVKEQSGQKCFLAQPVTLLDIYTHWQQTSELGRKRKAEEDAALQAKKTRVSDPISTSESSEEEEEAEAETAKATPRLASTNSSVLGADLPSSMKEKAKAETEKAGKTGNSMPHPATGKTVANLLSGKSPRKSAEPSANTTLVSETEEEGSVLAFGAAAKPGMVSAGQADSSSEDTSSSSDETDVEGKPSVKPAQVKASSVSTKESPARKAAPAPGKVGDVTPQVKGGALPPAKKAKKPEEESESSEEGSESEEEAPAGTRSQVKASEKILQVRAASAPAKGTPGKGATPAPPGKAGAVASQTKAGKPEEDSESSSEESSDSEEETPAAKALLQAKASGKTSQVGAASAPAKESPRKGAAPAPPGKTGPAVAKAQAGKREEDSQSSSEESDSEEEAPAQAKPSGKAPQVRAASAPAKESPRKGTAPAPPRKTGPAAAQVQVGKQEEDSGSSSEESDSDREALAAMNAAQVKPLGKSPQVKPASTMGMGPLGKGAGPVPPGKVGPATPSAQVGKWEEDSESSSEESSDSSDGEVPTAVAPAQEKSLGNILQAKPASSPAKGPPQKAGPVAVQVKAEKPMDNSESSEESSDSADSEEAPAVMTAAQAKPALKIPQTKACPKKTNTTASAKVAPVRVGTQAPRKAGTATSPAGSSPAVAGGTQRPAEDSSSSEESDSEEEKTGLAVTVGQAKSVGKGLQVKAASVPLKGSLGQGTAPVLPGKTGPTVTQVKAEKQEDSESSEEESDSEEAAASPAQVKTSVKKTQAKANPAAARAPSAKGTISAPGKVVTAAAQVKQRSPSKVKPPVRNPQNSTVLARGPASVPSVGKAVATAARAQTGPEEDSGSSEEESDSEEEVETLAQAKPSGKTPQIRAALAPAKESPRKGAAPTPPGKTGPSAAQAGKQDDSGSSSEESDSDGEAPAAVTSAQDQESS

Target ORF information:

RefSeq Version	XM_016954048.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016954048.2

ORF Insert Sequence:

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCAGAGAG CTCGGAAGAG GAGGAGGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCGAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCTTG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGT 
GAGGACACCT CCAGCTCCAG CGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCGCCAGCC 
AAGAAGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CGGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GAGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGTG AGGAGTCGTC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GTGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAAGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG ACTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAGGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCC ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCTGG GAAGGTGGGG CCTGCAACCC CCTCGGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCT 
GCCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CGTCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGTCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCGGC CTCAGTGCCT CTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAGTCAGAC AGTGAGGAGG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGTCAAA 
CAGAGATCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCGGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GTGGAGACGC TGGCTCAGGC GAAGCCTTCA GGGAAGACCC CCCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AAGCACCGGC AGCTGTGACC TCTGCCCAGG ACCAGGAGTC TTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_016954042.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016809531.2 Pan troglodytes treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OPc106675
Clone ID Related Accession (Same CDS sequence)	XM_016954042.2
Accession Version	XM_016954042.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4467bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	treacle protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036884.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016954042.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCAGAGAG CTCGGAAGAG GAGGAGGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCGAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCTTG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGT 
GAGGACACCT CCAGCTCCAG CGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCGCCAGCC 
AAGAAGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CGGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GAGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGTG AGGAGTCGTC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GTGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAAGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG ACTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAGGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCC ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCTGG GAAGGTGGGG CCTGCAACCC CCTCGGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCT 
GCCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CGTCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGTCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCGGC CTCAGTGCCT CTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAGTCAGAC AGTGAGGAGG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGTCAAA 
CAGAGATCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCGGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GTGGAGACGC TGGCTCAGGC GAAGCCTTCA GGGAAGACCC CCCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AAGCACCGGC AGCTGTGACC TCTGCCCAGG TGATTAAACC CCCTCTGATT 
TTTGTCGACC CTAATCGTAG TCCAGCTGGC CCAGCTGCTA CACCCGCACA AGCCCAGGCT 
GCAAGCACCC CGAGGAAGGC CCGAGCCTCG GAGAGCACAG CCAGGAGCTC CTCCTCCGAG 
AGCGAGGATG AGGACGTGAT CCCCGCTACA CAGTGCTTGA CTCCTGGCAT CAGAACCAAT 
GTGGTGACCA TGCCCACTGC CCACCCAAGA ATAGCCCCCA AAGCCAGCAT GGCTGGGGCC 
AGCAGCAGCA AGGAGTCCAG TCGGATATCA GATGGCAAGA AACAGGAGGG ACCAGCCACT 
CAGGTGTCAA AGAAGAACCC AGCTTCCCTC CCACTGACCC AGGCTACCCT GAAGGTCCTC 
GCCCAGAAAG CCAGTGAGGC TCAGCCTCCT GTTGCCAGGA CCCAGCCTTC AAGTGGGGTT 
GACAGTGCTG TGGGAACACT CCCTGCAACA AGTCCCCAGA GCAGCTCCGT CCAGGCCAAA 
GGGACCAACA AGCTCAGAAA ACCTAAGCTT CCTGAGGTCC AGCAGGCCAC CAAAGCCCCT 
GAGAGCTCAG ATGACAGTGA GGACAGCAGC GACAGTTCTT CAGGGAGTGA GGAAGATGCT 
GAAGGGCCCC AGGGGGCCAA GTCGGCCCAC ACGCTGGGTC CCACCCCCTC CAGGACAGAG 
ACCCTGGTGG AGGAGACCGC AGCAGAGTCC AGCGAGGATG ATGTGGTGGC GCCATCCCAG 
TCTCTCCTCT CAGGTTATAT GACCCCTGGA CTAACCCCAG CCAATTCCCA GGCCTCAAAA 
GCCACTCCCA AGCTAGACTC CAGCCCCTCA GTTTCCTCTA CTCTGGCCGC CAAAGATGAC 
CCAGATGGCA AGCAGGAGGC AAAGCCCCAA CAGGCAGCAG GCATGTTGTC CCCTAAAACA 
GGTGGAAAAG AGGCTGCTTC AGGCACCACA CCTCAGAAGT CCCGGAAGCC CAAGAAAGGG 
GCTGGGAACC CCCAAGCCTC AACCCTGGCG CTGCAAAGCA ACATCGCCCA GTGCCTCCTG 
GGCCAACCCT GGCCCCTGAA TGAGGCCCAG GTGCAGGCCT CAGTGGTGAA GGTCCTGACT 
GAGCTGCTGG AACAGGAAAG AAAGAAGGTG GTGGACACCA CCAAGGAGAG CAGCAGGAAG 
GGCTGGGAGA GCCGCAAGCG CAAGCTATCG GGAGACCAGC TGGCTGCCAG GACCCCCAGG 
AGCAAGAAGA AGAAGAAGCT GGGGGCCAGG GAAGGTGGGG AGGCCTCTGT TTCCCCAGAA 
AAGACCTCCA CGACTTCCAA GGGGAAAGCA AAGAGAGACA AAGCGAGTGG TGATGTCAAG 
GAGAAGAAAG GGAAGGGGTC TCTTGGCTCC CAAGGGGCCA AGGACGAGCC AGAAGAGGAG 
CTTCAGAAGG GGATGGGGAA GGTTGAAGGT GGAGATCAAA GCAACCCAAA GAGCAAGAAG 
GAGAAGAAGA AATCCGACAA GAGAAAAAAA GACAAAGAAA AAAAAGAAAA GAAGAAGAAA 
GCAAAAAAGG CCTCAACCAA AGATTCTGAG TCACCGTCCC AGAAGAAAAA GAAGAAAAAG 
AAGAAGACAG CAGAGCAGAC TGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016809531.2
CDS	104..4570
Translation	MAEARKRRELLPLIYHHLLRAGYVRAAREVKEQSGQKCFLAQPVTLLDIYTHWQQTSELGRKRKAEEDAALQAKKTRVSDPISTSESSEEEEEAEAETAKATPRLASTNSSVLGADLPSSMKEKAKAETEKAGKTGNSMPHPATGKTVANLLSGKSPRKSAEPSANTTLVSETEEEGSVLAFGAAAKPGMVSAGQADSSSEDTSSSSDETDVEGKPSVKPAQVKASSVSTKESPARKAAPAPGKVGDVTPQVKGGALPPAKKAKKPEEESESSEEGSESEEEAPAGTRSQVKASEKILQVRAASAPAKGTPGKGATPAPPGKAGAVASQTKAGKPEEDSESSSEESSDSEEETPAAKALLQAKASGKTSQVGAASAPAKESPRKGAAPAPPGKTGPAVAKAQAGKREEDSQSSSEESDSEEEAPAQAKPSGKAPQVRAASAPAKESPRKGTAPAPPRKTGPAAAQVQVGKQEEDSGSSSEESDSDREALAAMNAAQVKPLGKSPQVKPASTMGMGPLGKGAGPVPPGKVGPATPSAQVGKWEEDSESSSEESSDSSDGEVPTAVAPAQEKSLGNILQAKPASSPAKGPPQKAGPVAVQVKAEKPMDNSESSEESSDSADSEEAPAVMTAAQAKPALKIPQTKACPKKTNTTASAKVAPVRVGTQAPRKAGTATSPAGSSPAVAGGTQRPAEDSSSSEESDSEEEKTGLAVTVGQAKSVGKGLQVKAASVPLKGSLGQGTAPVLPGKTGPTVTQVKAEKQEDSESSEEESDSEEAAASPAQVKTSVKKTQAKANPAAARAPSAKGTISAPGKVVTAAAQVKQRSPSKVKPPVRNPQNSTVLARGPASVPSVGKAVATAARAQTGPEEDSGSSEEESDSEEEVETLAQAKPSGKTPQIRAALAPAKESPRKGAAPTPPGKTGPSAAQAGKQDDSGSSSEESDSDGEAPAAVTSAQVIKPPLIFVDPNRSPAGPAATPAQAQAASTPRKARASESTARSSSSESEDEDVIPATQCLTPGIRTNVVTMPTAHPRIAPKASMAGASSSKESSRISDGKKQEGPATQVSKKNPASLPLTQATLKVLAQKASEAQPPVARTQPSSGVDSAVGTLPATSPQSSSVQAKGTNKLRKPKLPEVQQATKAPESSDDSEDSSDSSSGSEEDAEGPQGAKSAHTLGPTPSRTETLVEETAAESSEDDVVAPSQSLLSGYMTPGLTPANSQASKATPKLDSSPSVSSTLAAKDDPDGKQEAKPQQAAGMLSPKTGGKEAASGTTPQKSRKPKKGAGNPQASTLALQSNIAQCLLGQPWPLNEAQVQASVVKVLTELLEQERKKVVDTTKESSRKGWESRKRKLSGDQLAARTPRSKKKKKLGAREGGEASVSPEKTSTTSKGKAKRDKASGDVKEKKGKGSLGSQGAKDEPEEELQKGMGKVEGGDQSNPKSKKEKKKSDKRKKDKEKKEKKKKAKKASTKDSESPSQKKKKKKKKTAEQTV

Target ORF information:

RefSeq Version	XM_016954042.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016954042.2

ORF Insert Sequence:

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCAGAGAG CTCGGAAGAG GAGGAGGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCGAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCTTG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGT 
GAGGACACCT CCAGCTCCAG CGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCGCCAGCC 
AAGAAGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CGGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GAGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGTG AGGAGTCGTC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GTGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAAGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG ACTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAGGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCC ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCTGG GAAGGTGGGG CCTGCAACCC CCTCGGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCT 
GCCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CGTCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGTCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCGGC CTCAGTGCCT CTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAGTCAGAC AGTGAGGAGG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGTCAAA 
CAGAGATCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCGGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GTGGAGACGC TGGCTCAGGC GAAGCCTTCA GGGAAGACCC CCCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AAGCACCGGC AGCTGTGACC TCTGCCCAGG TGATTAAACC CCCTCTGATT 
TTTGTCGACC CTAATCGTAG TCCAGCTGGC CCAGCTGCTA CACCCGCACA AGCCCAGGCT 
GCAAGCACCC CGAGGAAGGC CCGAGCCTCG GAGAGCACAG CCAGGAGCTC CTCCTCCGAG 
AGCGAGGATG AGGACGTGAT CCCCGCTACA CAGTGCTTGA CTCCTGGCAT CAGAACCAAT 
GTGGTGACCA TGCCCACTGC CCACCCAAGA ATAGCCCCCA AAGCCAGCAT GGCTGGGGCC 
AGCAGCAGCA AGGAGTCCAG TCGGATATCA GATGGCAAGA AACAGGAGGG ACCAGCCACT 
CAGGTGTCAA AGAAGAACCC AGCTTCCCTC CCACTGACCC AGGCTACCCT GAAGGTCCTC 
GCCCAGAAAG CCAGTGAGGC TCAGCCTCCT GTTGCCAGGA CCCAGCCTTC AAGTGGGGTT 
GACAGTGCTG TGGGAACACT CCCTGCAACA AGTCCCCAGA GCAGCTCCGT CCAGGCCAAA 
GGGACCAACA AGCTCAGAAA ACCTAAGCTT CCTGAGGTCC AGCAGGCCAC CAAAGCCCCT 
GAGAGCTCAG ATGACAGTGA GGACAGCAGC GACAGTTCTT CAGGGAGTGA GGAAGATGCT 
GAAGGGCCCC AGGGGGCCAA GTCGGCCCAC ACGCTGGGTC CCACCCCCTC CAGGACAGAG 
ACCCTGGTGG AGGAGACCGC AGCAGAGTCC AGCGAGGATG ATGTGGTGGC GCCATCCCAG 
TCTCTCCTCT CAGGTTATAT GACCCCTGGA CTAACCCCAG CCAATTCCCA GGCCTCAAAA 
GCCACTCCCA AGCTAGACTC CAGCCCCTCA GTTTCCTCTA CTCTGGCCGC CAAAGATGAC 
CCAGATGGCA AGCAGGAGGC AAAGCCCCAA CAGGCAGCAG GCATGTTGTC CCCTAAAACA 
GGTGGAAAAG AGGCTGCTTC AGGCACCACA CCTCAGAAGT CCCGGAAGCC CAAGAAAGGG 
GCTGGGAACC CCCAAGCCTC AACCCTGGCG CTGCAAAGCA ACATCGCCCA GTGCCTCCTG 
GGCCAACCCT GGCCCCTGAA TGAGGCCCAG GTGCAGGCCT CAGTGGTGAA GGTCCTGACT 
GAGCTGCTGG AACAGGAAAG AAAGAAGGTG GTGGACACCA CCAAGGAGAG CAGCAGGAAG 
GGCTGGGAGA GCCGCAAGCG CAAGCTATCG GGAGACCAGC TGGCTGCCAG GACCCCCAGG 
AGCAAGAAGA AGAAGAAGCT GGGGGCCAGG GAAGGTGGGG AGGCCTCTGT TTCCCCAGAA 
AAGACCTCCA CGACTTCCAA GGGGAAAGCA AAGAGAGACA AAGCGAGTGG TGATGTCAAG 
GAGAAGAAAG GGAAGGGGTC TCTTGGCTCC CAAGGGGCCA AGGACGAGCC AGAAGAGGAG 
CTTCAGAAGG GGATGGGGAA GGTTGAAGGT GGAGATCAAA GCAACCCAAA GAGCAAGAAG 
GAGAAGAAGA AATCCGACAA GAGAAAAAAA GACAAAGAAA AAAAAGAAAA GAAGAAGAAA 
GCAAAAAAGG CCTCAACCAA AGATTCTGAG TCACCGTCCC AGAAGAAAAA GAAGAAAAAG 
AAGAAGACAG CAGAGCAGAC TGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_016954043.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016809532.2 Pan troglodytes treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X4, mRNA.

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CloneID	OPc106676
Clone ID Related Accession (Same CDS sequence)	XM_016954043.2
Accession Version	XM_016954043.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4464bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	treacle protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036884.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016954043.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCAGAGAG CTCGGAAGAG GAGGAGGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCGAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCTTG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGT 
GAGGACACCT CCAGCTCCAG CGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCGCCAGCC 
AAGAAGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CGGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GAGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGTG AGGAGTCGTC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GTGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAAGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG ACTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAGGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCC ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCTGG GAAGGTGGGG CCTGCAACCC CCTCGGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCT 
GCCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CGTCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGTCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCGGC CTCAGTGCCT CTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAGTCAGAC AGTGAGGAGG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGTCAAA 
CAGAGATCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCGGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GTGGAGACGC TGGCTCAGGC GAAGCCTTCA GGGAAGACCC CCCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AAGCACCGGC AGCTGTGACC TCTGCCCAGA AGGACAGTAA CTCCAAACCT 
GCCAGAAGCA AGACCCTGGC CCCAGCACCC CCAGAGAGGA ACACGGAGGG GTCCTCGGAG 
AGCAGTGAGG AAGAGCTGCC ACTGACCCAG GTGATTAAAC CCCCTCTGAT TTTTGTCGAC 
CCTAATCGTA GTCCAGCTGG CCCAGCTGCT ACACCCGCAC AAGCCCAGGC TGCAAGCACC 
CCGAGGAAGG CCCGAGCCTC GGAGAGCACA GCCAGGAGCT CCTCCTCCGA GAGCGAGGAT 
GAGGACGTGA TCCCCGCTAC ACAGTGCTTG ACTCCTGGCA TCAGAACCAA TGTGGTGACC 
ATGCCCACTG CCCACCCAAG AATAGCCCCC AAAGCCAGCA TGGCTGGGGC CAGCAGCAGC 
AAGGAGTCCA GTCGGATATC AGATGGCAAG AAACAGGAGG GACCAGCCAC TCAGGTTGAC 
AGTGCTGTGG GAACACTCCC TGCAACAAGT CCCCAGAGCA GCTCCGTCCA GGCCAAAGGG 
ACCAACAAGC TCAGAAAACC TAAGCTTCCT GAGGTCCAGC AGGCCACCAA AGCCCCTGAG 
AGCTCAGATG ACAGTGAGGA CAGCAGCGAC AGTTCTTCAG GGAGTGAGGA AGATGCTGAA 
GGGCCCCAGG GGGCCAAGTC GGCCCACACG CTGGGTCCCA CCCCCTCCAG GACAGAGACC 
CTGGTGGAGG AGACCGCAGC AGAGTCCAGC GAGGATGATG TGGTGGCGCC ATCCCAGTCT 
CTCCTCTCAG GTTATATGAC CCCTGGACTA ACCCCAGCCA ATTCCCAGGC CTCAAAAGCC 
ACTCCCAAGC TAGACTCCAG CCCCTCAGTT TCCTCTACTC TGGCCGCCAA AGATGACCCA 
GATGGCAAGC AGGAGGCAAA GCCCCAACAG GCAGCAGGCA TGTTGTCCCC TAAAACAGGT 
GGAAAAGAGG CTGCTTCAGG CACCACACCT CAGAAGTCCC GGAAGCCCAA GAAAGGGGCT 
GGGAACCCCC AAGCCTCAAC CCTGGCGCTG CAAAGCAACA TCGCCCAGTG CCTCCTGGGC 
CAACCCTGGC CCCTGAATGA GGCCCAGGTG CAGGCCTCAG TGGTGAAGGT CCTGACTGAG 
CTGCTGGAAC AGGAAAGAAA GAAGGTGGTG GACACCACCA AGGAGAGCAG CAGGAAGGGC 
TGGGAGAGCC GCAAGCGCAA GCTATCGGGA GACCAGCTGG CTGCCAGGAC CCCCAGGAGC 
AAGAAGAAGA AGAAGCTGGG GGCCAGGGAA GGTGGGGAGG CCTCTGTTTC CCCAGAAAAG 
ACCTCCACGA CTTCCAAGGG GAAAGCAAAG AGAGACAAAG CGAGTGGTGA TGTCAAGGAG 
AAGAAAGGGA AGGGGTCTCT TGGCTCCCAA GGGGCCAAGG ACGAGCCAGA AGAGGAGCTT 
CAGAAGGGGA TGGGGAAGGT TGAAGGTGGA GATCAAAGCA ACCCAAAGAG CAAGAAGGAG 
AAGAAGAAAT CCGACAAGAG AAAAAAAGAC AAAGAAAAAA AAGAAAAGAA GAAGAAAGCA 
AAAAAGGCCT CAACCAAAGA TTCTGAGTCA CCGTCCCAGA AGAAAAAGAA GAAAAAGAAG 
AAGACAGCAG AGCAGACTGT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016809532.2
CDS	104..4567
Translation	MAEARKRRELLPLIYHHLLRAGYVRAAREVKEQSGQKCFLAQPVTLLDIYTHWQQTSELGRKRKAEEDAALQAKKTRVSDPISTSESSEEEEEAEAETAKATPRLASTNSSVLGADLPSSMKEKAKAETEKAGKTGNSMPHPATGKTVANLLSGKSPRKSAEPSANTTLVSETEEEGSVLAFGAAAKPGMVSAGQADSSSEDTSSSSDETDVEGKPSVKPAQVKASSVSTKESPARKAAPAPGKVGDVTPQVKGGALPPAKKAKKPEEESESSEEGSESEEEAPAGTRSQVKASEKILQVRAASAPAKGTPGKGATPAPPGKAGAVASQTKAGKPEEDSESSSEESSDSEEETPAAKALLQAKASGKTSQVGAASAPAKESPRKGAAPAPPGKTGPAVAKAQAGKREEDSQSSSEESDSEEEAPAQAKPSGKAPQVRAASAPAKESPRKGTAPAPPRKTGPAAAQVQVGKQEEDSGSSSEESDSDREALAAMNAAQVKPLGKSPQVKPASTMGMGPLGKGAGPVPPGKVGPATPSAQVGKWEEDSESSSEESSDSSDGEVPTAVAPAQEKSLGNILQAKPASSPAKGPPQKAGPVAVQVKAEKPMDNSESSEESSDSADSEEAPAVMTAAQAKPALKIPQTKACPKKTNTTASAKVAPVRVGTQAPRKAGTATSPAGSSPAVAGGTQRPAEDSSSSEESDSEEEKTGLAVTVGQAKSVGKGLQVKAASVPLKGSLGQGTAPVLPGKTGPTVTQVKAEKQEDSESSEEESDSEEAAASPAQVKTSVKKTQAKANPAAARAPSAKGTISAPGKVVTAAAQVKQRSPSKVKPPVRNPQNSTVLARGPASVPSVGKAVATAARAQTGPEEDSGSSEEESDSEEEVETLAQAKPSGKTPQIRAALAPAKESPRKGAAPTPPGKTGPSAAQAGKQDDSGSSSEESDSDGEAPAAVTSAQKDSNSKPARSKTLAPAPPERNTEGSSESSEEELPLTQVIKPPLIFVDPNRSPAGPAATPAQAQAASTPRKARASESTARSSSSESEDEDVIPATQCLTPGIRTNVVTMPTAHPRIAPKASMAGASSSKESSRISDGKKQEGPATQVDSAVGTLPATSPQSSSVQAKGTNKLRKPKLPEVQQATKAPESSDDSEDSSDSSSGSEEDAEGPQGAKSAHTLGPTPSRTETLVEETAAESSEDDVVAPSQSLLSGYMTPGLTPANSQASKATPKLDSSPSVSSTLAAKDDPDGKQEAKPQQAAGMLSPKTGGKEAASGTTPQKSRKPKKGAGNPQASTLALQSNIAQCLLGQPWPLNEAQVQASVVKVLTELLEQERKKVVDTTKESSRKGWESRKRKLSGDQLAARTPRSKKKKKLGAREGGEASVSPEKTSTTSKGKAKRDKASGDVKEKKGKGSLGSQGAKDEPEEELQKGMGKVEGGDQSNPKSKKEKKKSDKRKKDKEKKEKKKKAKKASTKDSESPSQKKKKKKKKTAEQTV

Target ORF information:

RefSeq Version	XM_016954043.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016954043.2

ORF Insert Sequence:

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCAGAGAG CTCGGAAGAG GAGGAGGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCGAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCTTG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGT 
GAGGACACCT CCAGCTCCAG CGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCGCCAGCC 
AAGAAGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CGGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GAGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGTG AGGAGTCGTC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GTGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAAGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG ACTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAGGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCC ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCTGG GAAGGTGGGG CCTGCAACCC CCTCGGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCT 
GCCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CGTCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGTCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCGGC CTCAGTGCCT CTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAGTCAGAC AGTGAGGAGG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGTCAAA 
CAGAGATCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCGGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GTGGAGACGC TGGCTCAGGC GAAGCCTTCA GGGAAGACCC CCCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AAGCACCGGC AGCTGTGACC TCTGCCCAGA AGGACAGTAA CTCCAAACCT 
GCCAGAAGCA AGACCCTGGC CCCAGCACCC CCAGAGAGGA ACACGGAGGG GTCCTCGGAG 
AGCAGTGAGG AAGAGCTGCC ACTGACCCAG GTGATTAAAC CCCCTCTGAT TTTTGTCGAC 
CCTAATCGTA GTCCAGCTGG CCCAGCTGCT ACACCCGCAC AAGCCCAGGC TGCAAGCACC 
CCGAGGAAGG CCCGAGCCTC GGAGAGCACA GCCAGGAGCT CCTCCTCCGA GAGCGAGGAT 
GAGGACGTGA TCCCCGCTAC ACAGTGCTTG ACTCCTGGCA TCAGAACCAA TGTGGTGACC 
ATGCCCACTG CCCACCCAAG AATAGCCCCC AAAGCCAGCA TGGCTGGGGC CAGCAGCAGC 
AAGGAGTCCA GTCGGATATC AGATGGCAAG AAACAGGAGG GACCAGCCAC TCAGGTTGAC 
AGTGCTGTGG GAACACTCCC TGCAACAAGT CCCCAGAGCA GCTCCGTCCA GGCCAAAGGG 
ACCAACAAGC TCAGAAAACC TAAGCTTCCT GAGGTCCAGC AGGCCACCAA AGCCCCTGAG 
AGCTCAGATG ACAGTGAGGA CAGCAGCGAC AGTTCTTCAG GGAGTGAGGA AGATGCTGAA 
GGGCCCCAGG GGGCCAAGTC GGCCCACACG CTGGGTCCCA CCCCCTCCAG GACAGAGACC 
CTGGTGGAGG AGACCGCAGC AGAGTCCAGC GAGGATGATG TGGTGGCGCC ATCCCAGTCT 
CTCCTCTCAG GTTATATGAC CCCTGGACTA ACCCCAGCCA ATTCCCAGGC CTCAAAAGCC 
ACTCCCAAGC TAGACTCCAG CCCCTCAGTT TCCTCTACTC TGGCCGCCAA AGATGACCCA 
GATGGCAAGC AGGAGGCAAA GCCCCAACAG GCAGCAGGCA TGTTGTCCCC TAAAACAGGT 
GGAAAAGAGG CTGCTTCAGG CACCACACCT CAGAAGTCCC GGAAGCCCAA GAAAGGGGCT 
GGGAACCCCC AAGCCTCAAC CCTGGCGCTG CAAAGCAACA TCGCCCAGTG CCTCCTGGGC 
CAACCCTGGC CCCTGAATGA GGCCCAGGTG CAGGCCTCAG TGGTGAAGGT CCTGACTGAG 
CTGCTGGAAC AGGAAAGAAA GAAGGTGGTG GACACCACCA AGGAGAGCAG CAGGAAGGGC 
TGGGAGAGCC GCAAGCGCAA GCTATCGGGA GACCAGCTGG CTGCCAGGAC CCCCAGGAGC 
AAGAAGAAGA AGAAGCTGGG GGCCAGGGAA GGTGGGGAGG CCTCTGTTTC CCCAGAAAAG 
ACCTCCACGA CTTCCAAGGG GAAAGCAAAG AGAGACAAAG CGAGTGGTGA TGTCAAGGAG 
AAGAAAGGGA AGGGGTCTCT TGGCTCCCAA GGGGCCAAGG ACGAGCCAGA AGAGGAGCTT 
CAGAAGGGGA TGGGGAAGGT TGAAGGTGGA GATCAAAGCA ACCCAAAGAG CAAGAAGGAG 
AAGAAGAAAT CCGACAAGAG AAAAAAAGAC AAAGAAAAAA AAGAAAAGAA GAAGAAAGCA 
AAAAAGGCCT CAACCAAAGA TTCTGAGTCA CCGTCCCAGA AGAAAAAGAA GAAAAAGAAG 
AAGACAGCAG AGCAGACTGT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_016954044.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016809533.2 Pan troglodytes treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OPc106677
Clone ID Related Accession (Same CDS sequence)	XM_016954044.2
Accession Version	XM_016954044.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4398bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	treacle protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036884.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016954044.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCAGAGAG CTCGGAAGAG GAGGAGGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCGAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCTTG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGT 
GAGGACACCT CCAGCTCCAG CGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCGCCAGCC 
AAGAAGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CGGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GAGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGTG AGGAGTCGTC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GTGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAAGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG ACTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAGGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCC ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCTGG GAAGGTGGGG CCTGCAACCC CCTCGGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCT 
GCCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CGTCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGTCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCGGC CTCAGTGCCT CTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAGTCAGAC AGTGAGGAGG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGTCAAA 
CAGAGATCTC CATCCAAGGC GAAGCCTTCA GGGAAGACCC CCCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AAGCACCGGC AGCTGTGACC TCTGCCCAGA AGGACAGTAA CTCCAAACCT 
GCCAGAAGCA AGACCCTGGC CCCAGCACCC CCAGAGAGGA ACACGGAGGG GTCCTCGGAG 
AGCAGTGAGG AAGAGCTGCC ACTGACCCAG GTGATTAAAC CCCCTCTGAT TTTTGTCGAC 
CCTAATCGTA GTCCAGCTGG CCCAGCTGCT ACACCCGCAC AAGCCCAGGC TGCAAGCACC 
CCGAGGAAGG CCCGAGCCTC GGAGAGCACA GCCAGGAGCT CCTCCTCCGA GAGCGAGGAT 
GAGGACGTGA TCCCCGCTAC ACAGTGCTTG ACTCCTGGCA TCAGAACCAA TGTGGTGACC 
ATGCCCACTG CCCACCCAAG AATAGCCCCC AAAGCCAGCA TGGCTGGGGC CAGCAGCAGC 
AAGGAGTCCA GTCGGATATC AGATGGCAAG AAACAGGAGG GACCAGCCAC TCAGGTGTCA 
AAGAAGAACC CAGCTTCCCT CCCACTGACC CAGGCTACCC TGAAGGTCCT CGCCCAGAAA 
GCCAGTGAGG CTCAGCCTCC TGTTGCCAGG ACCCAGCCTT CAAGTGGGGT TGACAGTGCT 
GTGGGAACAC TCCCTGCAAC AAGTCCCCAG AGCAGCTCCG TCCAGGCCAA AGGGACCAAC 
AAGCTCAGAA AACCTAAGCT TCCTGAGGTC CAGCAGGCCA CCAAAGCCCC TGAGAGCTCA 
GATGACAGTG AGGACAGCAG CGACAGTTCT TCAGGGAGTG AGGAAGATGC TGAAGGGCCC 
CAGGGGGCCA AGTCGGCCCA CACGCTGGGT CCCACCCCCT CCAGGACAGA GACCCTGGTG 
GAGGAGACCG CAGCAGAGTC CAGCGAGGAT GATGTGGTGG CGCCATCCCA GTCTCTCCTC 
TCAGGTTATA TGACCCCTGG ACTAACCCCA GCCAATTCCC AGGCCTCAAA AGCCACTCCC 
AAGCTAGACT CCAGCCCCTC AGTTTCCTCT ACTCTGGCCG CCAAAGATGA CCCAGATGGC 
AAGCAGGAGG CAAAGCCCCA ACAGGCAGCA GGCATGTTGT CCCCTAAAAC AGGTGGAAAA 
GAGGCTGCTT CAGGCACCAC ACCTCAGAAG TCCCGGAAGC CCAAGAAAGG GGCTGGGAAC 
CCCCAAGCCT CAACCCTGGC GCTGCAAAGC AACATCGCCC AGTGCCTCCT GGGCCAACCC 
TGGCCCCTGA ATGAGGCCCA GGTGCAGGCC TCAGTGGTGA AGGTCCTGAC TGAGCTGCTG 
GAACAGGAAA GAAAGAAGGT GGTGGACACC ACCAAGGAGA GCAGCAGGAA GGGCTGGGAG 
AGCCGCAAGC GCAAGCTATC GGGAGACCAG CTGGCTGCCA GGACCCCCAG GAGCAAGAAG 
AAGAAGAAGC TGGGGGCCAG GGAAGGTGGG GAGGCCTCTG TTTCCCCAGA AAAGACCTCC 
ACGACTTCCA AGGGGAAAGC AAAGAGAGAC AAAGCGAGTG GTGATGTCAA GGAGAAGAAA 
GGGAAGGGGT CTCTTGGCTC CCAAGGGGCC AAGGACGAGC CAGAAGAGGA GCTTCAGAAG 
GGGATGGGGA AGGTTGAAGG TGGAGATCAA AGCAACCCAA AGAGCAAGAA GGAGAAGAAG 
AAATCCGACA AGAGAAAAAA AGACAAAGAA AAAAAAGAAA AGAAGAAGAA AGCAAAAAAG 
GCCTCAACCA AAGATTCTGA GTCACCGTCC CAGAAGAAAA AGAAGAAAAA GAAGAAGACA 
GCAGAGCAGA CTGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016809533.2
CDS	104..4501
Translation	MAEARKRRELLPLIYHHLLRAGYVRAAREVKEQSGQKCFLAQPVTLLDIYTHWQQTSELGRKRKAEEDAALQAKKTRVSDPISTSESSEEEEEAEAETAKATPRLASTNSSVLGADLPSSMKEKAKAETEKAGKTGNSMPHPATGKTVANLLSGKSPRKSAEPSANTTLVSETEEEGSVLAFGAAAKPGMVSAGQADSSSEDTSSSSDETDVEGKPSVKPAQVKASSVSTKESPARKAAPAPGKVGDVTPQVKGGALPPAKKAKKPEEESESSEEGSESEEEAPAGTRSQVKASEKILQVRAASAPAKGTPGKGATPAPPGKAGAVASQTKAGKPEEDSESSSEESSDSEEETPAAKALLQAKASGKTSQVGAASAPAKESPRKGAAPAPPGKTGPAVAKAQAGKREEDSQSSSEESDSEEEAPAQAKPSGKAPQVRAASAPAKESPRKGTAPAPPRKTGPAAAQVQVGKQEEDSGSSSEESDSDREALAAMNAAQVKPLGKSPQVKPASTMGMGPLGKGAGPVPPGKVGPATPSAQVGKWEEDSESSSEESSDSSDGEVPTAVAPAQEKSLGNILQAKPASSPAKGPPQKAGPVAVQVKAEKPMDNSESSEESSDSADSEEAPAVMTAAQAKPALKIPQTKACPKKTNTTASAKVAPVRVGTQAPRKAGTATSPAGSSPAVAGGTQRPAEDSSSSEESDSEEEKTGLAVTVGQAKSVGKGLQVKAASVPLKGSLGQGTAPVLPGKTGPTVTQVKAEKQEDSESSEEESDSEEAAASPAQVKTSVKKTQAKANPAAARAPSAKGTISAPGKVVTAAAQVKQRSPSKAKPSGKTPQIRAALAPAKESPRKGAAPTPPGKTGPSAAQAGKQDDSGSSSEESDSDGEAPAAVTSAQKDSNSKPARSKTLAPAPPERNTEGSSESSEEELPLTQVIKPPLIFVDPNRSPAGPAATPAQAQAASTPRKARASESTARSSSSESEDEDVIPATQCLTPGIRTNVVTMPTAHPRIAPKASMAGASSSKESSRISDGKKQEGPATQVSKKNPASLPLTQATLKVLAQKASEAQPPVARTQPSSGVDSAVGTLPATSPQSSSVQAKGTNKLRKPKLPEVQQATKAPESSDDSEDSSDSSSGSEEDAEGPQGAKSAHTLGPTPSRTETLVEETAAESSEDDVVAPSQSLLSGYMTPGLTPANSQASKATPKLDSSPSVSSTLAAKDDPDGKQEAKPQQAAGMLSPKTGGKEAASGTTPQKSRKPKKGAGNPQASTLALQSNIAQCLLGQPWPLNEAQVQASVVKVLTELLEQERKKVVDTTKESSRKGWESRKRKLSGDQLAARTPRSKKKKKLGAREGGEASVSPEKTSTTSKGKAKRDKASGDVKEKKGKGSLGSQGAKDEPEEELQKGMGKVEGGDQSNPKSKKEKKKSDKRKKDKEKKEKKKKAKKASTKDSESPSQKKKKKKKKTAEQTV

Target ORF information:

RefSeq Version	XM_016954044.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016954044.2

ORF Insert Sequence:

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCAGAGAG CTCGGAAGAG GAGGAGGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCGAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCTTG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGT 
GAGGACACCT CCAGCTCCAG CGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCGCCAGCC 
AAGAAGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CGGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GAGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGTG AGGAGTCGTC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GTGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAAGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG ACTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAGGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCC ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCTGG GAAGGTGGGG CCTGCAACCC CCTCGGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCT 
GCCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CGTCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGTCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCGGC CTCAGTGCCT CTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAGTCAGAC AGTGAGGAGG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGTCAAA 
CAGAGATCTC CATCCAAGGC GAAGCCTTCA GGGAAGACCC CCCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AAGCACCGGC AGCTGTGACC TCTGCCCAGA AGGACAGTAA CTCCAAACCT 
GCCAGAAGCA AGACCCTGGC CCCAGCACCC CCAGAGAGGA ACACGGAGGG GTCCTCGGAG 
AGCAGTGAGG AAGAGCTGCC ACTGACCCAG GTGATTAAAC CCCCTCTGAT TTTTGTCGAC 
CCTAATCGTA GTCCAGCTGG CCCAGCTGCT ACACCCGCAC AAGCCCAGGC TGCAAGCACC 
CCGAGGAAGG CCCGAGCCTC GGAGAGCACA GCCAGGAGCT CCTCCTCCGA GAGCGAGGAT 
GAGGACGTGA TCCCCGCTAC ACAGTGCTTG ACTCCTGGCA TCAGAACCAA TGTGGTGACC 
ATGCCCACTG CCCACCCAAG AATAGCCCCC AAAGCCAGCA TGGCTGGGGC CAGCAGCAGC 
AAGGAGTCCA GTCGGATATC AGATGGCAAG AAACAGGAGG GACCAGCCAC TCAGGTGTCA 
AAGAAGAACC CAGCTTCCCT CCCACTGACC CAGGCTACCC TGAAGGTCCT CGCCCAGAAA 
GCCAGTGAGG CTCAGCCTCC TGTTGCCAGG ACCCAGCCTT CAAGTGGGGT TGACAGTGCT 
GTGGGAACAC TCCCTGCAAC AAGTCCCCAG AGCAGCTCCG TCCAGGCCAA AGGGACCAAC 
AAGCTCAGAA AACCTAAGCT TCCTGAGGTC CAGCAGGCCA CCAAAGCCCC TGAGAGCTCA 
GATGACAGTG AGGACAGCAG CGACAGTTCT TCAGGGAGTG AGGAAGATGC TGAAGGGCCC 
CAGGGGGCCA AGTCGGCCCA CACGCTGGGT CCCACCCCCT CCAGGACAGA GACCCTGGTG 
GAGGAGACCG CAGCAGAGTC CAGCGAGGAT GATGTGGTGG CGCCATCCCA GTCTCTCCTC 
TCAGGTTATA TGACCCCTGG ACTAACCCCA GCCAATTCCC AGGCCTCAAA AGCCACTCCC 
AAGCTAGACT CCAGCCCCTC AGTTTCCTCT ACTCTGGCCG CCAAAGATGA CCCAGATGGC 
AAGCAGGAGG CAAAGCCCCA ACAGGCAGCA GGCATGTTGT CCCCTAAAAC AGGTGGAAAA 
GAGGCTGCTT CAGGCACCAC ACCTCAGAAG TCCCGGAAGC CCAAGAAAGG GGCTGGGAAC 
CCCCAAGCCT CAACCCTGGC GCTGCAAAGC AACATCGCCC AGTGCCTCCT GGGCCAACCC 
TGGCCCCTGA ATGAGGCCCA GGTGCAGGCC TCAGTGGTGA AGGTCCTGAC TGAGCTGCTG 
GAACAGGAAA GAAAGAAGGT GGTGGACACC ACCAAGGAGA GCAGCAGGAA GGGCTGGGAG 
AGCCGCAAGC GCAAGCTATC GGGAGACCAG CTGGCTGCCA GGACCCCCAG GAGCAAGAAG 
AAGAAGAAGC TGGGGGCCAG GGAAGGTGGG GAGGCCTCTG TTTCCCCAGA AAAGACCTCC 
ACGACTTCCA AGGGGAAAGC AAAGAGAGAC AAAGCGAGTG GTGATGTCAA GGAGAAGAAA 
GGGAAGGGGT CTCTTGGCTC CCAAGGGGCC AAGGACGAGC CAGAAGAGGA GCTTCAGAAG 
GGGATGGGGA AGGTTGAAGG TGGAGATCAA AGCAACCCAA AGAGCAAGAA GGAGAAGAAG 
AAATCCGACA AGAGAAAAAA AGACAAAGAA AAAAAAGAAA AGAAGAAGAA AGCAAAAAAG 
GCCTCAACCA AAGATTCTGA GTCACCGTCC CAGAAGAAAA AGAAGAAAAA GAAGAAGACA 
GCAGAGCAGA CTGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_016954046.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016809535.2 Pan troglodytes treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OPc106678
Clone ID Related Accession (Same CDS sequence)	XM_016954046.2
Accession Version	XM_016954046.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4353bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	treacle protein isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036884.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016954046.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCAGAGAG CTCGGAAGAG GAGGAGGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCGAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCTTG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGT 
GAGGACACCT CCAGCTCCAG CGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCGCCAGCC 
AAGAAGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CGGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GAGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGTG AGGAGTCGTC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GTGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAAGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG ACTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAGGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCC ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCTGG GAAGGTGGGG CCTGCAACCC CCTCGGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCT 
GCCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CGTCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGTCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCGGC CTCAGTGCCT CTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAGTCAGAC AGTGAGGAGG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGTCAAA 
CAGAGATCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCGGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GTGGAGACGC TGGCTCAGGC GAAGCCTTCA GGGAAGACCC CCCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AAGCACCGGC AGCTGTGACC TCTGCCCAGG TGATTAAACC CCCTCTGATT 
TTTGTCGACC CTAATCGTAG TCCAGCTGGC CCAGCTGCTA CACCCGCACA AGCCCAGGCT 
GCAAGCACCC CGAGGAAGGC CCGAGCCTCG GAGAGCACAG CCAGGAGCTC CTCCTCCGAG 
AGCGAGGATG AGGACGTGAT CCCCGCTACA CAGTGCTTGA CTCCTGGCAT CAGAACCAAT 
GTGGTGACCA TGCCCACTGC CCACCCAAGA ATAGCCCCCA AAGCCAGCAT GGCTGGGGCC 
AGCAGCAGCA AGGAGTCCAG TCGGATATCA GATGGCAAGA AACAGGAGGG ACCAGCCACT 
CAGGTTGACA GTGCTGTGGG AACACTCCCT GCAACAAGTC CCCAGAGCAG CTCCGTCCAG 
GCCAAAGGGA CCAACAAGCT CAGAAAACCT AAGCTTCCTG AGGTCCAGCA GGCCACCAAA 
GCCCCTGAGA GCTCAGATGA CAGTGAGGAC AGCAGCGACA GTTCTTCAGG GAGTGAGGAA 
GATGCTGAAG GGCCCCAGGG GGCCAAGTCG GCCCACACGC TGGGTCCCAC CCCCTCCAGG 
ACAGAGACCC TGGTGGAGGA GACCGCAGCA GAGTCCAGCG AGGATGATGT GGTGGCGCCA 
TCCCAGTCTC TCCTCTCAGG TTATATGACC CCTGGACTAA CCCCAGCCAA TTCCCAGGCC 
TCAAAAGCCA CTCCCAAGCT AGACTCCAGC CCCTCAGTTT CCTCTACTCT GGCCGCCAAA 
GATGACCCAG ATGGCAAGCA GGAGGCAAAG CCCCAACAGG CAGCAGGCAT GTTGTCCCCT 
AAAACAGGTG GAAAAGAGGC TGCTTCAGGC ACCACACCTC AGAAGTCCCG GAAGCCCAAG 
AAAGGGGCTG GGAACCCCCA AGCCTCAACC CTGGCGCTGC AAAGCAACAT CGCCCAGTGC 
CTCCTGGGCC AACCCTGGCC CCTGAATGAG GCCCAGGTGC AGGCCTCAGT GGTGAAGGTC 
CTGACTGAGC TGCTGGAACA GGAAAGAAAG AAGGTGGTGG ACACCACCAA GGAGAGCAGC 
AGGAAGGGCT GGGAGAGCCG CAAGCGCAAG CTATCGGGAG ACCAGCTGGC TGCCAGGACC 
CCCAGGAGCA AGAAGAAGAA GAAGCTGGGG GCCAGGGAAG GTGGGGAGGC CTCTGTTTCC 
CCAGAAAAGA CCTCCACGAC TTCCAAGGGG AAAGCAAAGA GAGACAAAGC GAGTGGTGAT 
GTCAAGGAGA AGAAAGGGAA GGGGTCTCTT GGCTCCCAAG GGGCCAAGGA CGAGCCAGAA 
GAGGAGCTTC AGAAGGGGAT GGGGAAGGTT GAAGGTGGAG ATCAAAGCAA CCCAAAGAGC 
AAGAAGGAGA AGAAGAAATC CGACAAGAGA AAAAAAGACA AAGAAAAAAA AGAAAAGAAG 
AAGAAAGCAA AAAAGGCCTC AACCAAAGAT TCTGAGTCAC CGTCCCAGAA GAAAAAGAAG 
AAAAAGAAGA AGACAGCAGA GCAGACTGTA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016809535.2
CDS	104..4456
Translation	MAEARKRRELLPLIYHHLLRAGYVRAAREVKEQSGQKCFLAQPVTLLDIYTHWQQTSELGRKRKAEEDAALQAKKTRVSDPISTSESSEEEEEAEAETAKATPRLASTNSSVLGADLPSSMKEKAKAETEKAGKTGNSMPHPATGKTVANLLSGKSPRKSAEPSANTTLVSETEEEGSVLAFGAAAKPGMVSAGQADSSSEDTSSSSDETDVEGKPSVKPAQVKASSVSTKESPARKAAPAPGKVGDVTPQVKGGALPPAKKAKKPEEESESSEEGSESEEEAPAGTRSQVKASEKILQVRAASAPAKGTPGKGATPAPPGKAGAVASQTKAGKPEEDSESSSEESSDSEEETPAAKALLQAKASGKTSQVGAASAPAKESPRKGAAPAPPGKTGPAVAKAQAGKREEDSQSSSEESDSEEEAPAQAKPSGKAPQVRAASAPAKESPRKGTAPAPPRKTGPAAAQVQVGKQEEDSGSSSEESDSDREALAAMNAAQVKPLGKSPQVKPASTMGMGPLGKGAGPVPPGKVGPATPSAQVGKWEEDSESSSEESSDSSDGEVPTAVAPAQEKSLGNILQAKPASSPAKGPPQKAGPVAVQVKAEKPMDNSESSEESSDSADSEEAPAVMTAAQAKPALKIPQTKACPKKTNTTASAKVAPVRVGTQAPRKAGTATSPAGSSPAVAGGTQRPAEDSSSSEESDSEEEKTGLAVTVGQAKSVGKGLQVKAASVPLKGSLGQGTAPVLPGKTGPTVTQVKAEKQEDSESSEEESDSEEAAASPAQVKTSVKKTQAKANPAAARAPSAKGTISAPGKVVTAAAQVKQRSPSKVKPPVRNPQNSTVLARGPASVPSVGKAVATAARAQTGPEEDSGSSEEESDSEEEVETLAQAKPSGKTPQIRAALAPAKESPRKGAAPTPPGKTGPSAAQAGKQDDSGSSSEESDSDGEAPAAVTSAQVIKPPLIFVDPNRSPAGPAATPAQAQAASTPRKARASESTARSSSSESEDEDVIPATQCLTPGIRTNVVTMPTAHPRIAPKASMAGASSSKESSRISDGKKQEGPATQVDSAVGTLPATSPQSSSVQAKGTNKLRKPKLPEVQQATKAPESSDDSEDSSDSSSGSEEDAEGPQGAKSAHTLGPTPSRTETLVEETAAESSEDDVVAPSQSLLSGYMTPGLTPANSQASKATPKLDSSPSVSSTLAAKDDPDGKQEAKPQQAAGMLSPKTGGKEAASGTTPQKSRKPKKGAGNPQASTLALQSNIAQCLLGQPWPLNEAQVQASVVKVLTELLEQERKKVVDTTKESSRKGWESRKRKLSGDQLAARTPRSKKKKKLGAREGGEASVSPEKTSTTSKGKAKRDKASGDVKEKKGKGSLGSQGAKDEPEEELQKGMGKVEGGDQSNPKSKKEKKKSDKRKKDKEKKEKKKKAKKASTKDSESPSQKKKKKKKKTAEQTV

Target ORF information:

RefSeq Version	XM_016954046.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016954046.2

ORF Insert Sequence:

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCAGAGAG CTCGGAAGAG GAGGAGGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCGAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCTTG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGT 
GAGGACACCT CCAGCTCCAG CGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCGCCAGCC 
AAGAAGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CGGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GAGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGTG AGGAGTCGTC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GTGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAAGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG ACTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAGGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCC ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCTGG GAAGGTGGGG CCTGCAACCC CCTCGGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCT 
GCCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CGTCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGTCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCGGC CTCAGTGCCT CTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAGTCAGAC AGTGAGGAGG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGTCAAA 
CAGAGATCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCGGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GTGGAGACGC TGGCTCAGGC GAAGCCTTCA GGGAAGACCC CCCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AAGCACCGGC AGCTGTGACC TCTGCCCAGG TGATTAAACC CCCTCTGATT 
TTTGTCGACC CTAATCGTAG TCCAGCTGGC CCAGCTGCTA CACCCGCACA AGCCCAGGCT 
GCAAGCACCC CGAGGAAGGC CCGAGCCTCG GAGAGCACAG CCAGGAGCTC CTCCTCCGAG 
AGCGAGGATG AGGACGTGAT CCCCGCTACA CAGTGCTTGA CTCCTGGCAT CAGAACCAAT 
GTGGTGACCA TGCCCACTGC CCACCCAAGA ATAGCCCCCA AAGCCAGCAT GGCTGGGGCC 
AGCAGCAGCA AGGAGTCCAG TCGGATATCA GATGGCAAGA AACAGGAGGG ACCAGCCACT 
CAGGTTGACA GTGCTGTGGG AACACTCCCT GCAACAAGTC CCCAGAGCAG CTCCGTCCAG 
GCCAAAGGGA CCAACAAGCT CAGAAAACCT AAGCTTCCTG AGGTCCAGCA GGCCACCAAA 
GCCCCTGAGA GCTCAGATGA CAGTGAGGAC AGCAGCGACA GTTCTTCAGG GAGTGAGGAA 
GATGCTGAAG GGCCCCAGGG GGCCAAGTCG GCCCACACGC TGGGTCCCAC CCCCTCCAGG 
ACAGAGACCC TGGTGGAGGA GACCGCAGCA GAGTCCAGCG AGGATGATGT GGTGGCGCCA 
TCCCAGTCTC TCCTCTCAGG TTATATGACC CCTGGACTAA CCCCAGCCAA TTCCCAGGCC 
TCAAAAGCCA CTCCCAAGCT AGACTCCAGC CCCTCAGTTT CCTCTACTCT GGCCGCCAAA 
GATGACCCAG ATGGCAAGCA GGAGGCAAAG CCCCAACAGG CAGCAGGCAT GTTGTCCCCT 
AAAACAGGTG GAAAAGAGGC TGCTTCAGGC ACCACACCTC AGAAGTCCCG GAAGCCCAAG 
AAAGGGGCTG GGAACCCCCA AGCCTCAACC CTGGCGCTGC AAAGCAACAT CGCCCAGTGC 
CTCCTGGGCC AACCCTGGCC CCTGAATGAG GCCCAGGTGC AGGCCTCAGT GGTGAAGGTC 
CTGACTGAGC TGCTGGAACA GGAAAGAAAG AAGGTGGTGG ACACCACCAA GGAGAGCAGC 
AGGAAGGGCT GGGAGAGCCG CAAGCGCAAG CTATCGGGAG ACCAGCTGGC TGCCAGGACC 
CCCAGGAGCA AGAAGAAGAA GAAGCTGGGG GCCAGGGAAG GTGGGGAGGC CTCTGTTTCC 
CCAGAAAAGA CCTCCACGAC TTCCAAGGGG AAAGCAAAGA GAGACAAAGC GAGTGGTGAT 
GTCAAGGAGA AGAAAGGGAA GGGGTCTCTT GGCTCCCAAG GGGCCAAGGA CGAGCCAGAA 
GAGGAGCTTC AGAAGGGGAT GGGGAAGGTT GAAGGTGGAG ATCAAAGCAA CCCAAAGAGC 
AAGAAGGAGA AGAAGAAATC CGACAAGAGA AAAAAAGACA AAGAAAAAAA AGAAAAGAAG 
AAGAAAGCAA AAAAGGCCTC AACCAAAGAT TCTGAGTCAC CGTCCCAGAA GAAAAAGAAG 
AAAAAGAAGA AGACAGCAGA GCAGACTGTA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_016954047.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016809536.2 Pan troglodytes treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X7, mRNA.

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CloneID	OPc106679
Clone ID Related Accession (Same CDS sequence)	XM_016954047.2
Accession Version	XM_016954047.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4236bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	treacle protein isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036884.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016954047.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCAGAGAG CTCGGAAGAG GAGGAGGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCGAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCTTG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGT 
GAGGACACCT CCAGCTCCAG CGATGAGACA GACGTGGAGG TAAAGGCCTC TGAAAAAATT 
CTCCAGGTCA GAGCTGCCTC GGCCCCTGCC AAGGGGACCC CTGGGAAAGG GGCTACCCCA 
GCACCCCCTG GGAAGGCAGG AGCTGTAGCC TCCCAGACCA AGGCAGGGAA GCCAGAGGAG 
GACTCAGAGA GCAGCAGTGA GGAGTCGTCT GACAGTGAGG AGGAGACGCC AGCTGCCAAG 
GCCCTGCTTC AGGCGAAGGC CTCAGGAAAA ACCTCTCAGG TCGGAGCTGC CTCAGCCCCT 
GCCAAGGAGT CCCCCAGGAA AGGAGCTGCC CCAGCGCCCC CTGGGAAGAC AGGGCCTGCA 
GTTGCCAAGG CCCAGGCGGG GAAGCGGGAG GAGGACTCGC AGAGCAGCAG TGAGGAATCG 
GACAGTGAGG AGGAGGCGCC TGCTCAGGCG AAGCCTTCAG GGAAGGCCCC CCAAGTCAGA 
GCCGCCTCGG CCCCTGCCAA GGAGTCCCCC AGGAAAGGGA CTGCCCCAGC ACCTCCTAGG 
AAAACAGGGC CTGCAGCCGC CCAGGTCCAG GTGGGGAAGC AGGAGGAGGA CTCAGGAAGC 
AGCAGCGAGG AGTCAGACAG TGACAGAGAG GCACTGGCAG CCATGAATGC AGCTCAGGTG 
AAGCCCTTGG GGAAAAGCCC CCAGGTGAAA CCTGCCTCCA CCATGGGCAT GGGGCCCTTG 
GGGAAAGGCG CCGGCCCAGT GCCACCTGGG AAGGTGGGGC CTGCAACCCC CTCGGCCCAG 
GTGGGGAAGT GGGAGGAGGA CTCAGAGAGC AGTAGTGAGG AGTCATCAGA CAGCAGTGAT 
GGAGAGGTGC CCACAGCTGT GGCCCCGGCT CAGGAAAAGT CCTTGGGGAA CATCCTCCAG 
GCCAAACCTG CCTCCAGTCC TGCCAAGGGG CCCCCTCAGA AGGCAGGGCC TGTAGCCGTC 
CAGGTCAAGG CTGAAAAGCC CATGGACAAC TCGGAGAGCA GCGAGGAGTC GTCGGACAGT 
GCGGACAGTG AGGAGGCACC AGCAGTCATG ACTGCAGCTC AGGCAAAACC AGCTCTGAAA 
ATTCCTCAGA CCAAGGCCTG CCCAAAGAAA ACCAATACCA CTGCATCTGC CAAGGTCGCC 
CCTGTGCGAG TGGGCACCCA AGCCCCCCGG AAAGCAGGAA CTGCGACTTC TCCAGCAGGC 
TCATCCCCAG CTGTGGCTGG GGGCACCCAG AGACCAGCAG AGGATTCTTC AAGCAGTGAG 
GAATCAGATA GTGAGGAAGA GAAGACAGGT CTTGCAGTAA CCGTGGGACA GGCAAAGTCT 
GTGGGGAAAG GCCTCCAGGT GAAAGCGGCC TCAGTGCCTC TCAAGGGGTC CTTGGGGCAA 
GGGACTGCTC CAGTACTCCC TGGGAAGACG GGGCCTACAG TCACCCAGGT GAAAGCTGAA 
AAGCAGGAAG ACTCTGAGAG CAGTGAGGAG GAGTCAGACA GTGAGGAGGC AGCTGCATCT 
CCAGCACAGG TGAAAACCTC AGTAAAGAAA ACCCAGGCCA AAGCCAACCC AGCTGCCGCC 
AGAGCACCTT CAGCAAAAGG GACAATTTCA GCCCCTGGAA AAGTTGTCAC TGCAGCTGCT 
CAAGTCAAAC AGAGATCTCC ATCCAAGGTG AAGCCACCAG TGAGAAACCC CCAGAACAGT 
ACCGTCTTGG CGAGGGGCCC AGCATCTGTG CCATCTGTGG GGAAGGCCGT GGCTACAGCA 
GCTCGGGCCC AGACAGGGCC AGAGGAGGAC TCAGGGAGCA GTGAGGAGGA GTCAGACAGT 
GAGGAGGAGG TGGAGACGCT GGCTCAGGCG AAGCCTTCAG GGAAGACCCC CCAGATCAGA 
GCTGCCTTGG CTCCTGCCAA GGAGTCCCCC AGGAAAGGGG CTGCCCCAAC ACCTCCTGGG 
AAGACAGGGC CTTCGGCTGC CCAGGCAGGG AAGCAGGATG ACTCAGGGAG CAGCAGCGAG 
GAATCAGACA GTGATGGGGA AGCACCGGCA GCTGTGACCT CTGCCCAGGT GATTAAACCC 
CCTCTGATTT TTGTCGACCC TAATCGTAGT CCAGCTGGCC CAGCTGCTAC ACCCGCACAA 
GCCCAGGCTG CAAGCACCCC GAGGAAGGCC CGAGCCTCGG AGAGCACAGC CAGGAGCTCC 
TCCTCCGAGA GCGAGGATGA GGACGTGATC CCCGCTACAC AGTGCTTGAC TCCTGGCATC 
AGAACCAATG TGGTGACCAT GCCCACTGCC CACCCAAGAA TAGCCCCCAA AGCCAGCATG 
GCTGGGGCCA GCAGCAGCAA GGAGTCCAGT CGGATATCAG ATGGCAAGAA ACAGGAGGGA 
CCAGCCACTC AGGTGTCAAA GAAGAACCCA GCTTCCCTCC CACTGACCCA GGCTACCCTG 
AAGGTCCTCG CCCAGAAAGC CAGTGAGGCT CAGCCTCCTG TTGCCAGGAC CCAGCCTTCA 
AGTGGGGTTG ACAGTGCTGT GGGAACACTC CCTGCAACAA GTCCCCAGAG CAGCTCCGTC 
CAGGCCAAAG GGACCAACAA GCTCAGAAAA CCTAAGCTTC CTGAGGTCCA GCAGGCCACC 
AAAGCCCCTG AGAGCTCAGA TGACAGTGAG GACAGCAGCG ACAGTTCTTC AGGGAGTGAG 
GAAGATGCTG AAGGGCCCCA GGGGGCCAAG TCGGCCCACA CGCTGGGTCC CACCCCCTCC 
AGGACAGAGA CCCTGGTGGA GGAGACCGCA GCAGAGTCCA GCGAGGATGA TGTGGTGGCG 
CCATCCCAGT CTCTCCTCTC AGGTTATATG ACCCCTGGAC TAACCCCAGC CAATTCCCAG 
GCCTCAAAAG CCACTCCCAA GCTAGACTCC AGCCCCTCAG TTTCCTCTAC TCTGGCCGCC 
AAAGATGACC CAGATGGCAA GCAGGAGGCA AAGCCCCAAC AGGCAGCAGG CATGTTGTCC 
CCTAAAACAG GTGGAAAAGA GGCTGCTTCA GGCACCACAC CTCAGAAGTC CCGGAAGCCC 
AAGAAAGGGG CTGGGAACCC CCAAGCCTCA ACCCTGGCGC TGCAAAGCAA CATCGCCCAG 
TGCCTCCTGG GCCAACCCTG GCCCCTGAAT GAGGCCCAGG TGCAGGCCTC AGTGGTGAAG 
GTCCTGACTG AGCTGCTGGA ACAGGAAAGA AAGAAGGTGG TGGACACCAC CAAGGAGAGC 
AGCAGGAAGG GCTGGGAGAG CCGCAAGCGC AAGCTATCGG GAGACCAGCT GGCTGCCAGG 
ACCCCCAGGA GCAAGAAGAA GAAGAAGCTG GGGGCCAGGG AAGGTGGGGA GGCCTCTGTT 
TCCCCAGAAA AGACCTCCAC GACTTCCAAG GGGAAAGCAA AGAGAGACAA AGCGAGTGGT 
GATGTCAAGG AGAAGAAAGG GAAGGGGTCT CTTGGCTCCC AAGGGGCCAA GGACGAGCCA 
GAAGAGGAGC TTCAGAAGGG GATGGGGAAG GTTGAAGGTG GAGATCAAAG CAACCCAAAG 
AGCAAGAAGG AGAAGAAGAA ATCCGACAAG AGAAAAAAAG ACAAAGAAAA AAAAGAAAAG 
AAGAAGAAAG CAAAAAAGGC CTCAACCAAA GATTCTGAGT CACCGTCCCA GAAGAAAAAG 
AAGAAAAAGA AGAAGACAGC AGAGCAGACT GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016809536.2
CDS	104..4339
Translation	MAEARKRRELLPLIYHHLLRAGYVRAAREVKEQSGQKCFLAQPVTLLDIYTHWQQTSELGRKRKAEEDAALQAKKTRVSDPISTSESSEEEEEAEAETAKATPRLASTNSSVLGADLPSSMKEKAKAETEKAGKTGNSMPHPATGKTVANLLSGKSPRKSAEPSANTTLVSETEEEGSVLAFGAAAKPGMVSAGQADSSSEDTSSSSDETDVEVKASEKILQVRAASAPAKGTPGKGATPAPPGKAGAVASQTKAGKPEEDSESSSEESSDSEEETPAAKALLQAKASGKTSQVGAASAPAKESPRKGAAPAPPGKTGPAVAKAQAGKREEDSQSSSEESDSEEEAPAQAKPSGKAPQVRAASAPAKESPRKGTAPAPPRKTGPAAAQVQVGKQEEDSGSSSEESDSDREALAAMNAAQVKPLGKSPQVKPASTMGMGPLGKGAGPVPPGKVGPATPSAQVGKWEEDSESSSEESSDSSDGEVPTAVAPAQEKSLGNILQAKPASSPAKGPPQKAGPVAVQVKAEKPMDNSESSEESSDSADSEEAPAVMTAAQAKPALKIPQTKACPKKTNTTASAKVAPVRVGTQAPRKAGTATSPAGSSPAVAGGTQRPAEDSSSSEESDSEEEKTGLAVTVGQAKSVGKGLQVKAASVPLKGSLGQGTAPVLPGKTGPTVTQVKAEKQEDSESSEEESDSEEAAASPAQVKTSVKKTQAKANPAAARAPSAKGTISAPGKVVTAAAQVKQRSPSKVKPPVRNPQNSTVLARGPASVPSVGKAVATAARAQTGPEEDSGSSEEESDSEEEVETLAQAKPSGKTPQIRAALAPAKESPRKGAAPTPPGKTGPSAAQAGKQDDSGSSSEESDSDGEAPAAVTSAQVIKPPLIFVDPNRSPAGPAATPAQAQAASTPRKARASESTARSSSSESEDEDVIPATQCLTPGIRTNVVTMPTAHPRIAPKASMAGASSSKESSRISDGKKQEGPATQVSKKNPASLPLTQATLKVLAQKASEAQPPVARTQPSSGVDSAVGTLPATSPQSSSVQAKGTNKLRKPKLPEVQQATKAPESSDDSEDSSDSSSGSEEDAEGPQGAKSAHTLGPTPSRTETLVEETAAESSEDDVVAPSQSLLSGYMTPGLTPANSQASKATPKLDSSPSVSSTLAAKDDPDGKQEAKPQQAAGMLSPKTGGKEAASGTTPQKSRKPKKGAGNPQASTLALQSNIAQCLLGQPWPLNEAQVQASVVKVLTELLEQERKKVVDTTKESSRKGWESRKRKLSGDQLAARTPRSKKKKKLGAREGGEASVSPEKTSTTSKGKAKRDKASGDVKEKKGKGSLGSQGAKDEPEEELQKGMGKVEGGDQSNPKSKKEKKKSDKRKKDKEKKEKKKKAKKASTKDSESPSQKKKKKKKKTAEQTV

Target ORF information:

RefSeq Version	XM_016954047.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016954047.2

ORF Insert Sequence:

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCAGAGAG CTCGGAAGAG GAGGAGGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCGAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCTTG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGT 
GAGGACACCT CCAGCTCCAG CGATGAGACA GACGTGGAGG TAAAGGCCTC TGAAAAAATT 
CTCCAGGTCA GAGCTGCCTC GGCCCCTGCC AAGGGGACCC CTGGGAAAGG GGCTACCCCA 
GCACCCCCTG GGAAGGCAGG AGCTGTAGCC TCCCAGACCA AGGCAGGGAA GCCAGAGGAG 
GACTCAGAGA GCAGCAGTGA GGAGTCGTCT GACAGTGAGG AGGAGACGCC AGCTGCCAAG 
GCCCTGCTTC AGGCGAAGGC CTCAGGAAAA ACCTCTCAGG TCGGAGCTGC CTCAGCCCCT 
GCCAAGGAGT CCCCCAGGAA AGGAGCTGCC CCAGCGCCCC CTGGGAAGAC AGGGCCTGCA 
GTTGCCAAGG CCCAGGCGGG GAAGCGGGAG GAGGACTCGC AGAGCAGCAG TGAGGAATCG 
GACAGTGAGG AGGAGGCGCC TGCTCAGGCG AAGCCTTCAG GGAAGGCCCC CCAAGTCAGA 
GCCGCCTCGG CCCCTGCCAA GGAGTCCCCC AGGAAAGGGA CTGCCCCAGC ACCTCCTAGG 
AAAACAGGGC CTGCAGCCGC CCAGGTCCAG GTGGGGAAGC AGGAGGAGGA CTCAGGAAGC 
AGCAGCGAGG AGTCAGACAG TGACAGAGAG GCACTGGCAG CCATGAATGC AGCTCAGGTG 
AAGCCCTTGG GGAAAAGCCC CCAGGTGAAA CCTGCCTCCA CCATGGGCAT GGGGCCCTTG 
GGGAAAGGCG CCGGCCCAGT GCCACCTGGG AAGGTGGGGC CTGCAACCCC CTCGGCCCAG 
GTGGGGAAGT GGGAGGAGGA CTCAGAGAGC AGTAGTGAGG AGTCATCAGA CAGCAGTGAT 
GGAGAGGTGC CCACAGCTGT GGCCCCGGCT CAGGAAAAGT CCTTGGGGAA CATCCTCCAG 
GCCAAACCTG CCTCCAGTCC TGCCAAGGGG CCCCCTCAGA AGGCAGGGCC TGTAGCCGTC 
CAGGTCAAGG CTGAAAAGCC CATGGACAAC TCGGAGAGCA GCGAGGAGTC GTCGGACAGT 
GCGGACAGTG AGGAGGCACC AGCAGTCATG ACTGCAGCTC AGGCAAAACC AGCTCTGAAA 
ATTCCTCAGA CCAAGGCCTG CCCAAAGAAA ACCAATACCA CTGCATCTGC CAAGGTCGCC 
CCTGTGCGAG TGGGCACCCA AGCCCCCCGG AAAGCAGGAA CTGCGACTTC TCCAGCAGGC 
TCATCCCCAG CTGTGGCTGG GGGCACCCAG AGACCAGCAG AGGATTCTTC AAGCAGTGAG 
GAATCAGATA GTGAGGAAGA GAAGACAGGT CTTGCAGTAA CCGTGGGACA GGCAAAGTCT 
GTGGGGAAAG GCCTCCAGGT GAAAGCGGCC TCAGTGCCTC TCAAGGGGTC CTTGGGGCAA 
GGGACTGCTC CAGTACTCCC TGGGAAGACG GGGCCTACAG TCACCCAGGT GAAAGCTGAA 
AAGCAGGAAG ACTCTGAGAG CAGTGAGGAG GAGTCAGACA GTGAGGAGGC AGCTGCATCT 
CCAGCACAGG TGAAAACCTC AGTAAAGAAA ACCCAGGCCA AAGCCAACCC AGCTGCCGCC 
AGAGCACCTT CAGCAAAAGG GACAATTTCA GCCCCTGGAA AAGTTGTCAC TGCAGCTGCT 
CAAGTCAAAC AGAGATCTCC ATCCAAGGTG AAGCCACCAG TGAGAAACCC CCAGAACAGT 
ACCGTCTTGG CGAGGGGCCC AGCATCTGTG CCATCTGTGG GGAAGGCCGT GGCTACAGCA 
GCTCGGGCCC AGACAGGGCC AGAGGAGGAC TCAGGGAGCA GTGAGGAGGA GTCAGACAGT 
GAGGAGGAGG TGGAGACGCT GGCTCAGGCG AAGCCTTCAG GGAAGACCCC CCAGATCAGA 
GCTGCCTTGG CTCCTGCCAA GGAGTCCCCC AGGAAAGGGG CTGCCCCAAC ACCTCCTGGG 
AAGACAGGGC CTTCGGCTGC CCAGGCAGGG AAGCAGGATG ACTCAGGGAG CAGCAGCGAG 
GAATCAGACA GTGATGGGGA AGCACCGGCA GCTGTGACCT CTGCCCAGGT GATTAAACCC 
CCTCTGATTT TTGTCGACCC TAATCGTAGT CCAGCTGGCC CAGCTGCTAC ACCCGCACAA 
GCCCAGGCTG CAAGCACCCC GAGGAAGGCC CGAGCCTCGG AGAGCACAGC CAGGAGCTCC 
TCCTCCGAGA GCGAGGATGA GGACGTGATC CCCGCTACAC AGTGCTTGAC TCCTGGCATC 
AGAACCAATG TGGTGACCAT GCCCACTGCC CACCCAAGAA TAGCCCCCAA AGCCAGCATG 
GCTGGGGCCA GCAGCAGCAA GGAGTCCAGT CGGATATCAG ATGGCAAGAA ACAGGAGGGA 
CCAGCCACTC AGGTGTCAAA GAAGAACCCA GCTTCCCTCC CACTGACCCA GGCTACCCTG 
AAGGTCCTCG CCCAGAAAGC CAGTGAGGCT CAGCCTCCTG TTGCCAGGAC CCAGCCTTCA 
AGTGGGGTTG ACAGTGCTGT GGGAACACTC CCTGCAACAA GTCCCCAGAG CAGCTCCGTC 
CAGGCCAAAG GGACCAACAA GCTCAGAAAA CCTAAGCTTC CTGAGGTCCA GCAGGCCACC 
AAAGCCCCTG AGAGCTCAGA TGACAGTGAG GACAGCAGCG ACAGTTCTTC AGGGAGTGAG 
GAAGATGCTG AAGGGCCCCA GGGGGCCAAG TCGGCCCACA CGCTGGGTCC CACCCCCTCC 
AGGACAGAGA CCCTGGTGGA GGAGACCGCA GCAGAGTCCA GCGAGGATGA TGTGGTGGCG 
CCATCCCAGT CTCTCCTCTC AGGTTATATG ACCCCTGGAC TAACCCCAGC CAATTCCCAG 
GCCTCAAAAG CCACTCCCAA GCTAGACTCC AGCCCCTCAG TTTCCTCTAC TCTGGCCGCC 
AAAGATGACC CAGATGGCAA GCAGGAGGCA AAGCCCCAAC AGGCAGCAGG CATGTTGTCC 
CCTAAAACAG GTGGAAAAGA GGCTGCTTCA GGCACCACAC CTCAGAAGTC CCGGAAGCCC 
AAGAAAGGGG CTGGGAACCC CCAAGCCTCA ACCCTGGCGC TGCAAAGCAA CATCGCCCAG 
TGCCTCCTGG GCCAACCCTG GCCCCTGAAT GAGGCCCAGG TGCAGGCCTC AGTGGTGAAG 
GTCCTGACTG AGCTGCTGGA ACAGGAAAGA AAGAAGGTGG TGGACACCAC CAAGGAGAGC 
AGCAGGAAGG GCTGGGAGAG CCGCAAGCGC AAGCTATCGG GAGACCAGCT GGCTGCCAGG 
ACCCCCAGGA GCAAGAAGAA GAAGAAGCTG GGGGCCAGGG AAGGTGGGGA GGCCTCTGTT 
TCCCCAGAAA AGACCTCCAC GACTTCCAAG GGGAAAGCAA AGAGAGACAA AGCGAGTGGT 
GATGTCAAGG AGAAGAAAGG GAAGGGGTCT CTTGGCTCCC AAGGGGCCAA GGACGAGCCA 
GAAGAGGAGC TTCAGAAGGG GATGGGGAAG GTTGAAGGTG GAGATCAAAG CAACCCAAAG 
AGCAAGAAGG AGAAGAAGAA ATCCGACAAG AGAAAAAAAG ACAAAGAAAA AAAAGAAAAG 
AAGAAGAAAG CAAAAAAGGC CTCAACCAAA GATTCTGAGT CACCGTCCCA GAAGAAAAAG 
AAGAAAAAGA AGAAGACAGC AGAGCAGACT GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_016954040.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016809529.2 Pan troglodytes treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X1, mRNA.

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CloneID	OPc106673
Clone ID Related Accession (Same CDS sequence)	XM_016954040.2
Accession Version	XM_016954040.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4578bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	treacle protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036884.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016954040.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCAGAGAG CTCGGAAGAG GAGGAGGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCGAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCTTG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGT 
GAGGACACCT CCAGCTCCAG CGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCGCCAGCC 
AAGAAGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CGGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GAGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGTG AGGAGTCGTC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GTGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAAGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG ACTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAGGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCC ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCTGG GAAGGTGGGG CCTGCAACCC CCTCGGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCT 
GCCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CGTCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGTCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCGGC CTCAGTGCCT CTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAGTCAGAC AGTGAGGAGG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGTCAAA 
CAGAGATCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCGGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GTGGAGACGC TGGCTCAGGC GAAGCCTTCA GGGAAGACCC CCCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AAGCACCGGC AGCTGTGACC TCTGCCCAGA AGGACAGTAA CTCCAAACCT 
GCCAGAAGCA AGACCCTGGC CCCAGCACCC CCAGAGAGGA ACACGGAGGG GTCCTCGGAG 
AGCAGTGAGG AAGAGCTGCC ACTGACCCAG GTGATTAAAC CCCCTCTGAT TTTTGTCGAC 
CCTAATCGTA GTCCAGCTGG CCCAGCTGCT ACACCCGCAC AAGCCCAGGC TGCAAGCACC 
CCGAGGAAGG CCCGAGCCTC GGAGAGCACA GCCAGGAGCT CCTCCTCCGA GAGCGAGGAT 
GAGGACGTGA TCCCCGCTAC ACAGTGCTTG ACTCCTGGCA TCAGAACCAA TGTGGTGACC 
ATGCCCACTG CCCACCCAAG AATAGCCCCC AAAGCCAGCA TGGCTGGGGC CAGCAGCAGC 
AAGGAGTCCA GTCGGATATC AGATGGCAAG AAACAGGAGG GACCAGCCAC TCAGGTGTCA 
AAGAAGAACC CAGCTTCCCT CCCACTGACC CAGGCTACCC TGAAGGTCCT CGCCCAGAAA 
GCCAGTGAGG CTCAGCCTCC TGTTGCCAGG ACCCAGCCTT CAAGTGGGGT TGACAGTGCT 
GTGGGAACAC TCCCTGCAAC AAGTCCCCAG AGCAGCTCCG TCCAGGCCAA AGGGACCAAC 
AAGCTCAGAA AACCTAAGCT TCCTGAGGTC CAGCAGGCCA CCAAAGCCCC TGAGAGCTCA 
GATGACAGTG AGGACAGCAG CGACAGTTCT TCAGGGAGTG AGGAAGATGC TGAAGGGCCC 
CAGGGGGCCA AGTCGGCCCA CACGCTGGGT CCCACCCCCT CCAGGACAGA GACCCTGGTG 
GAGGAGACCG CAGCAGAGTC CAGCGAGGAT GATGTGGTGG CGCCATCCCA GTCTCTCCTC 
TCAGGTTATA TGACCCCTGG ACTAACCCCA GCCAATTCCC AGGCCTCAAA AGCCACTCCC 
AAGCTAGACT CCAGCCCCTC AGTTTCCTCT ACTCTGGCCG CCAAAGATGA CCCAGATGGC 
AAGCAGGAGG CAAAGCCCCA ACAGGCAGCA GGCATGTTGT CCCCTAAAAC AGGTGGAAAA 
GAGGCTGCTT CAGGCACCAC ACCTCAGAAG TCCCGGAAGC CCAAGAAAGG GGCTGGGAAC 
CCCCAAGCCT CAACCCTGGC GCTGCAAAGC AACATCGCCC AGTGCCTCCT GGGCCAACCC 
TGGCCCCTGA ATGAGGCCCA GGTGCAGGCC TCAGTGGTGA AGGTCCTGAC TGAGCTGCTG 
GAACAGGAAA GAAAGAAGGT GGTGGACACC ACCAAGGAGA GCAGCAGGAA GGGCTGGGAG 
AGCCGCAAGC GCAAGCTATC GGGAGACCAG CTGGCTGCCA GGACCCCCAG GAGCAAGAAG 
AAGAAGAAGC TGGGGGCCAG GGAAGGTGGG GAGGCCTCTG TTTCCCCAGA AAAGACCTCC 
ACGACTTCCA AGGGGAAAGC AAAGAGAGAC AAAGCGAGTG GTGATGTCAA GGAGAAGAAA 
GGGAAGGGGT CTCTTGGCTC CCAAGGGGCC AAGGACGAGC CAGAAGAGGA GCTTCAGAAG 
GGGATGGGGA AGGTTGAAGG TGGAGATCAA AGCAACCCAA AGAGCAAGAA GGAGAAGAAG 
AAATCCGACA AGAGAAAAAA AGACAAAGAA AAAAAAGAAA AGAAGAAGAA AGCAAAAAAG 
GCCTCAACCA AAGATTCTGA GTCACCGTCC CAGAAGAAAA AGAAGAAAAA GAAGAAGACA 
GCAGAGCAGA CTGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016809529.2
CDS	104..4681
Translation	MAEARKRRELLPLIYHHLLRAGYVRAAREVKEQSGQKCFLAQPVTLLDIYTHWQQTSELGRKRKAEEDAALQAKKTRVSDPISTSESSEEEEEAEAETAKATPRLASTNSSVLGADLPSSMKEKAKAETEKAGKTGNSMPHPATGKTVANLLSGKSPRKSAEPSANTTLVSETEEEGSVLAFGAAAKPGMVSAGQADSSSEDTSSSSDETDVEGKPSVKPAQVKASSVSTKESPARKAAPAPGKVGDVTPQVKGGALPPAKKAKKPEEESESSEEGSESEEEAPAGTRSQVKASEKILQVRAASAPAKGTPGKGATPAPPGKAGAVASQTKAGKPEEDSESSSEESSDSEEETPAAKALLQAKASGKTSQVGAASAPAKESPRKGAAPAPPGKTGPAVAKAQAGKREEDSQSSSEESDSEEEAPAQAKPSGKAPQVRAASAPAKESPRKGTAPAPPRKTGPAAAQVQVGKQEEDSGSSSEESDSDREALAAMNAAQVKPLGKSPQVKPASTMGMGPLGKGAGPVPPGKVGPATPSAQVGKWEEDSESSSEESSDSSDGEVPTAVAPAQEKSLGNILQAKPASSPAKGPPQKAGPVAVQVKAEKPMDNSESSEESSDSADSEEAPAVMTAAQAKPALKIPQTKACPKKTNTTASAKVAPVRVGTQAPRKAGTATSPAGSSPAVAGGTQRPAEDSSSSEESDSEEEKTGLAVTVGQAKSVGKGLQVKAASVPLKGSLGQGTAPVLPGKTGPTVTQVKAEKQEDSESSEEESDSEEAAASPAQVKTSVKKTQAKANPAAARAPSAKGTISAPGKVVTAAAQVKQRSPSKVKPPVRNPQNSTVLARGPASVPSVGKAVATAARAQTGPEEDSGSSEEESDSEEEVETLAQAKPSGKTPQIRAALAPAKESPRKGAAPTPPGKTGPSAAQAGKQDDSGSSSEESDSDGEAPAAVTSAQKDSNSKPARSKTLAPAPPERNTEGSSESSEEELPLTQVIKPPLIFVDPNRSPAGPAATPAQAQAASTPRKARASESTARSSSSESEDEDVIPATQCLTPGIRTNVVTMPTAHPRIAPKASMAGASSSKESSRISDGKKQEGPATQVSKKNPASLPLTQATLKVLAQKASEAQPPVARTQPSSGVDSAVGTLPATSPQSSSVQAKGTNKLRKPKLPEVQQATKAPESSDDSEDSSDSSSGSEEDAEGPQGAKSAHTLGPTPSRTETLVEETAAESSEDDVVAPSQSLLSGYMTPGLTPANSQASKATPKLDSSPSVSSTLAAKDDPDGKQEAKPQQAAGMLSPKTGGKEAASGTTPQKSRKPKKGAGNPQASTLALQSNIAQCLLGQPWPLNEAQVQASVVKVLTELLEQERKKVVDTTKESSRKGWESRKRKLSGDQLAARTPRSKKKKKLGAREGGEASVSPEKTSTTSKGKAKRDKASGDVKEKKGKGSLGSQGAKDEPEEELQKGMGKVEGGDQSNPKSKKEKKKSDKRKKDKEKKEKKKKAKKASTKDSESPSQKKKKKKKKTAEQTV

Target ORF information:

RefSeq Version	XM_016954040.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016954040.2

ORF Insert Sequence:

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCAGAGAG CTCGGAAGAG GAGGAGGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCGAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCTTG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGT 
GAGGACACCT CCAGCTCCAG CGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCGCCAGCC 
AAGAAGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CGGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GAGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGTG AGGAGTCGTC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GTGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAAGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG ACTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAGGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCC ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCTGG GAAGGTGGGG CCTGCAACCC CCTCGGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCT 
GCCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CGTCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGTCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCGGC CTCAGTGCCT CTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAGTCAGAC AGTGAGGAGG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGTCAAA 
CAGAGATCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCGGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GTGGAGACGC TGGCTCAGGC GAAGCCTTCA GGGAAGACCC CCCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AAGCACCGGC AGCTGTGACC TCTGCCCAGA AGGACAGTAA CTCCAAACCT 
GCCAGAAGCA AGACCCTGGC CCCAGCACCC CCAGAGAGGA ACACGGAGGG GTCCTCGGAG 
AGCAGTGAGG AAGAGCTGCC ACTGACCCAG GTGATTAAAC CCCCTCTGAT TTTTGTCGAC 
CCTAATCGTA GTCCAGCTGG CCCAGCTGCT ACACCCGCAC AAGCCCAGGC TGCAAGCACC 
CCGAGGAAGG CCCGAGCCTC GGAGAGCACA GCCAGGAGCT CCTCCTCCGA GAGCGAGGAT 
GAGGACGTGA TCCCCGCTAC ACAGTGCTTG ACTCCTGGCA TCAGAACCAA TGTGGTGACC 
ATGCCCACTG CCCACCCAAG AATAGCCCCC AAAGCCAGCA TGGCTGGGGC CAGCAGCAGC 
AAGGAGTCCA GTCGGATATC AGATGGCAAG AAACAGGAGG GACCAGCCAC TCAGGTGTCA 
AAGAAGAACC CAGCTTCCCT CCCACTGACC CAGGCTACCC TGAAGGTCCT CGCCCAGAAA 
GCCAGTGAGG CTCAGCCTCC TGTTGCCAGG ACCCAGCCTT CAAGTGGGGT TGACAGTGCT 
GTGGGAACAC TCCCTGCAAC AAGTCCCCAG AGCAGCTCCG TCCAGGCCAA AGGGACCAAC 
AAGCTCAGAA AACCTAAGCT TCCTGAGGTC CAGCAGGCCA CCAAAGCCCC TGAGAGCTCA 
GATGACAGTG AGGACAGCAG CGACAGTTCT TCAGGGAGTG AGGAAGATGC TGAAGGGCCC 
CAGGGGGCCA AGTCGGCCCA CACGCTGGGT CCCACCCCCT CCAGGACAGA GACCCTGGTG 
GAGGAGACCG CAGCAGAGTC CAGCGAGGAT GATGTGGTGG CGCCATCCCA GTCTCTCCTC 
TCAGGTTATA TGACCCCTGG ACTAACCCCA GCCAATTCCC AGGCCTCAAA AGCCACTCCC 
AAGCTAGACT CCAGCCCCTC AGTTTCCTCT ACTCTGGCCG CCAAAGATGA CCCAGATGGC 
AAGCAGGAGG CAAAGCCCCA ACAGGCAGCA GGCATGTTGT CCCCTAAAAC AGGTGGAAAA 
GAGGCTGCTT CAGGCACCAC ACCTCAGAAG TCCCGGAAGC CCAAGAAAGG GGCTGGGAAC 
CCCCAAGCCT CAACCCTGGC GCTGCAAAGC AACATCGCCC AGTGCCTCCT GGGCCAACCC 
TGGCCCCTGA ATGAGGCCCA GGTGCAGGCC TCAGTGGTGA AGGTCCTGAC TGAGCTGCTG 
GAACAGGAAA GAAAGAAGGT GGTGGACACC ACCAAGGAGA GCAGCAGGAA GGGCTGGGAG 
AGCCGCAAGC GCAAGCTATC GGGAGACCAG CTGGCTGCCA GGACCCCCAG GAGCAAGAAG 
AAGAAGAAGC TGGGGGCCAG GGAAGGTGGG GAGGCCTCTG TTTCCCCAGA AAAGACCTCC 
ACGACTTCCA AGGGGAAAGC AAAGAGAGAC AAAGCGAGTG GTGATGTCAA GGAGAAGAAA 
GGGAAGGGGT CTCTTGGCTC CCAAGGGGCC AAGGACGAGC CAGAAGAGGA GCTTCAGAAG 
GGGATGGGGA AGGTTGAAGG TGGAGATCAA AGCAACCCAA AGAGCAAGAA GGAGAAGAAG 
AAATCCGACA AGAGAAAAAA AGACAAAGAA AAAAAAGAAA AGAAGAAGAA AGCAAAAAAG 
GCCTCAACCA AAGATTCTGA GTCACCGTCC CAGAAGAAAA AGAAGAAAAA GAAGAAGACA 
GCAGAGCAGA CTGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_016954041.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016809530.2 Pan troglodytes treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPc106674
Clone ID Related Accession (Same CDS sequence)	XM_016954041.2
Accession Version	XM_016954041.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4575bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	treacle protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036884.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016954041.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCAGAGAG CTCGGAAGAG GAGGAGGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCGAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCTTG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGT 
GAGGACACCT CCAGCTCCAG CGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCGCCAGCC 
AAGAAGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CGGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GAGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGTG AGGAGTCGTC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GTGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAAGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG ACTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAGGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCC ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCTGG GAAGGTGGGG CCTGCAACCC CCTCGGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCT 
GCCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CGTCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGTCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCGGC CTCAGTGCCT CTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAGTCAGAC AGTGAGGAGG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGTCAAA 
CAGAGATCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCGGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GTGGAGACGC TGGCTCAGGC GAAGCCTTCA GGGAAGACCC CCCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AAGCACCGGC AGCTGTGACC TCTGCCCAGG ACAGTAACTC CAAACCTGCC 
AGAAGCAAGA CCCTGGCCCC AGCACCCCCA GAGAGGAACA CGGAGGGGTC CTCGGAGAGC 
AGTGAGGAAG AGCTGCCACT GACCCAGGTG ATTAAACCCC CTCTGATTTT TGTCGACCCT 
AATCGTAGTC CAGCTGGCCC AGCTGCTACA CCCGCACAAG CCCAGGCTGC AAGCACCCCG 
AGGAAGGCCC GAGCCTCGGA GAGCACAGCC AGGAGCTCCT CCTCCGAGAG CGAGGATGAG 
GACGTGATCC CCGCTACACA GTGCTTGACT CCTGGCATCA GAACCAATGT GGTGACCATG 
CCCACTGCCC ACCCAAGAAT AGCCCCCAAA GCCAGCATGG CTGGGGCCAG CAGCAGCAAG 
GAGTCCAGTC GGATATCAGA TGGCAAGAAA CAGGAGGGAC CAGCCACTCA GGTGTCAAAG 
AAGAACCCAG CTTCCCTCCC ACTGACCCAG GCTACCCTGA AGGTCCTCGC CCAGAAAGCC 
AGTGAGGCTC AGCCTCCTGT TGCCAGGACC CAGCCTTCAA GTGGGGTTGA CAGTGCTGTG 
GGAACACTCC CTGCAACAAG TCCCCAGAGC AGCTCCGTCC AGGCCAAAGG GACCAACAAG 
CTCAGAAAAC CTAAGCTTCC TGAGGTCCAG CAGGCCACCA AAGCCCCTGA GAGCTCAGAT 
GACAGTGAGG ACAGCAGCGA CAGTTCTTCA GGGAGTGAGG AAGATGCTGA AGGGCCCCAG 
GGGGCCAAGT CGGCCCACAC GCTGGGTCCC ACCCCCTCCA GGACAGAGAC CCTGGTGGAG 
GAGACCGCAG CAGAGTCCAG CGAGGATGAT GTGGTGGCGC CATCCCAGTC TCTCCTCTCA 
GGTTATATGA CCCCTGGACT AACCCCAGCC AATTCCCAGG CCTCAAAAGC CACTCCCAAG 
CTAGACTCCA GCCCCTCAGT TTCCTCTACT CTGGCCGCCA AAGATGACCC AGATGGCAAG 
CAGGAGGCAA AGCCCCAACA GGCAGCAGGC ATGTTGTCCC CTAAAACAGG TGGAAAAGAG 
GCTGCTTCAG GCACCACACC TCAGAAGTCC CGGAAGCCCA AGAAAGGGGC TGGGAACCCC 
CAAGCCTCAA CCCTGGCGCT GCAAAGCAAC ATCGCCCAGT GCCTCCTGGG CCAACCCTGG 
CCCCTGAATG AGGCCCAGGT GCAGGCCTCA GTGGTGAAGG TCCTGACTGA GCTGCTGGAA 
CAGGAAAGAA AGAAGGTGGT GGACACCACC AAGGAGAGCA GCAGGAAGGG CTGGGAGAGC 
CGCAAGCGCA AGCTATCGGG AGACCAGCTG GCTGCCAGGA CCCCCAGGAG CAAGAAGAAG 
AAGAAGCTGG GGGCCAGGGA AGGTGGGGAG GCCTCTGTTT CCCCAGAAAA GACCTCCACG 
ACTTCCAAGG GGAAAGCAAA GAGAGACAAA GCGAGTGGTG ATGTCAAGGA GAAGAAAGGG 
AAGGGGTCTC TTGGCTCCCA AGGGGCCAAG GACGAGCCAG AAGAGGAGCT TCAGAAGGGG 
ATGGGGAAGG TTGAAGGTGG AGATCAAAGC AACCCAAAGA GCAAGAAGGA GAAGAAGAAA 
TCCGACAAGA GAAAAAAAGA CAAAGAAAAA AAAGAAAAGA AGAAGAAAGC AAAAAAGGCC 
TCAACCAAAG ATTCTGAGTC ACCGTCCCAG AAGAAAAAGA AGAAAAAGAA GAAGACAGCA 
GAGCAGACTG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016809530.2
CDS	104..4678
Translation	MAEARKRRELLPLIYHHLLRAGYVRAAREVKEQSGQKCFLAQPVTLLDIYTHWQQTSELGRKRKAEEDAALQAKKTRVSDPISTSESSEEEEEAEAETAKATPRLASTNSSVLGADLPSSMKEKAKAETEKAGKTGNSMPHPATGKTVANLLSGKSPRKSAEPSANTTLVSETEEEGSVLAFGAAAKPGMVSAGQADSSSEDTSSSSDETDVEGKPSVKPAQVKASSVSTKESPARKAAPAPGKVGDVTPQVKGGALPPAKKAKKPEEESESSEEGSESEEEAPAGTRSQVKASEKILQVRAASAPAKGTPGKGATPAPPGKAGAVASQTKAGKPEEDSESSSEESSDSEEETPAAKALLQAKASGKTSQVGAASAPAKESPRKGAAPAPPGKTGPAVAKAQAGKREEDSQSSSEESDSEEEAPAQAKPSGKAPQVRAASAPAKESPRKGTAPAPPRKTGPAAAQVQVGKQEEDSGSSSEESDSDREALAAMNAAQVKPLGKSPQVKPASTMGMGPLGKGAGPVPPGKVGPATPSAQVGKWEEDSESSSEESSDSSDGEVPTAVAPAQEKSLGNILQAKPASSPAKGPPQKAGPVAVQVKAEKPMDNSESSEESSDSADSEEAPAVMTAAQAKPALKIPQTKACPKKTNTTASAKVAPVRVGTQAPRKAGTATSPAGSSPAVAGGTQRPAEDSSSSEESDSEEEKTGLAVTVGQAKSVGKGLQVKAASVPLKGSLGQGTAPVLPGKTGPTVTQVKAEKQEDSESSEEESDSEEAAASPAQVKTSVKKTQAKANPAAARAPSAKGTISAPGKVVTAAAQVKQRSPSKVKPPVRNPQNSTVLARGPASVPSVGKAVATAARAQTGPEEDSGSSEEESDSEEEVETLAQAKPSGKTPQIRAALAPAKESPRKGAAPTPPGKTGPSAAQAGKQDDSGSSSEESDSDGEAPAAVTSAQDSNSKPARSKTLAPAPPERNTEGSSESSEEELPLTQVIKPPLIFVDPNRSPAGPAATPAQAQAASTPRKARASESTARSSSSESEDEDVIPATQCLTPGIRTNVVTMPTAHPRIAPKASMAGASSSKESSRISDGKKQEGPATQVSKKNPASLPLTQATLKVLAQKASEAQPPVARTQPSSGVDSAVGTLPATSPQSSSVQAKGTNKLRKPKLPEVQQATKAPESSDDSEDSSDSSSGSEEDAEGPQGAKSAHTLGPTPSRTETLVEETAAESSEDDVVAPSQSLLSGYMTPGLTPANSQASKATPKLDSSPSVSSTLAAKDDPDGKQEAKPQQAAGMLSPKTGGKEAASGTTPQKSRKPKKGAGNPQASTLALQSNIAQCLLGQPWPLNEAQVQASVVKVLTELLEQERKKVVDTTKESSRKGWESRKRKLSGDQLAARTPRSKKKKKLGAREGGEASVSPEKTSTTSKGKAKRDKASGDVKEKKGKGSLGSQGAKDEPEEELQKGMGKVEGGDQSNPKSKKEKKKSDKRKKDKEKKEKKKKAKKASTKDSESPSQKKKKKKKKTAEQTV

Target ORF information:

RefSeq Version	XM_016954041.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016954041.2

ORF Insert Sequence:

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCAGAGAG CTCGGAAGAG GAGGAGGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCGAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCTTG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGT 
GAGGACACCT CCAGCTCCAG CGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCGCCAGCC 
AAGAAGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CGGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GAGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGTG AGGAGTCGTC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GTGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAAGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG ACTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAGGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCC ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCTGG GAAGGTGGGG CCTGCAACCC CCTCGGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCT 
GCCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CGTCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGTCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCGGC CTCAGTGCCT CTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAGTCAGAC AGTGAGGAGG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGTCAAA 
CAGAGATCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCGGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GTGGAGACGC TGGCTCAGGC GAAGCCTTCA GGGAAGACCC CCCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AAGCACCGGC AGCTGTGACC TCTGCCCAGG ACAGTAACTC CAAACCTGCC 
AGAAGCAAGA CCCTGGCCCC AGCACCCCCA GAGAGGAACA CGGAGGGGTC CTCGGAGAGC 
AGTGAGGAAG AGCTGCCACT GACCCAGGTG ATTAAACCCC CTCTGATTTT TGTCGACCCT 
AATCGTAGTC CAGCTGGCCC AGCTGCTACA CCCGCACAAG CCCAGGCTGC AAGCACCCCG 
AGGAAGGCCC GAGCCTCGGA GAGCACAGCC AGGAGCTCCT CCTCCGAGAG CGAGGATGAG 
GACGTGATCC CCGCTACACA GTGCTTGACT CCTGGCATCA GAACCAATGT GGTGACCATG 
CCCACTGCCC ACCCAAGAAT AGCCCCCAAA GCCAGCATGG CTGGGGCCAG CAGCAGCAAG 
GAGTCCAGTC GGATATCAGA TGGCAAGAAA CAGGAGGGAC CAGCCACTCA GGTGTCAAAG 
AAGAACCCAG CTTCCCTCCC ACTGACCCAG GCTACCCTGA AGGTCCTCGC CCAGAAAGCC 
AGTGAGGCTC AGCCTCCTGT TGCCAGGACC CAGCCTTCAA GTGGGGTTGA CAGTGCTGTG 
GGAACACTCC CTGCAACAAG TCCCCAGAGC AGCTCCGTCC AGGCCAAAGG GACCAACAAG 
CTCAGAAAAC CTAAGCTTCC TGAGGTCCAG CAGGCCACCA AAGCCCCTGA GAGCTCAGAT 
GACAGTGAGG ACAGCAGCGA CAGTTCTTCA GGGAGTGAGG AAGATGCTGA AGGGCCCCAG 
GGGGCCAAGT CGGCCCACAC GCTGGGTCCC ACCCCCTCCA GGACAGAGAC CCTGGTGGAG 
GAGACCGCAG CAGAGTCCAG CGAGGATGAT GTGGTGGCGC CATCCCAGTC TCTCCTCTCA 
GGTTATATGA CCCCTGGACT AACCCCAGCC AATTCCCAGG CCTCAAAAGC CACTCCCAAG 
CTAGACTCCA GCCCCTCAGT TTCCTCTACT CTGGCCGCCA AAGATGACCC AGATGGCAAG 
CAGGAGGCAA AGCCCCAACA GGCAGCAGGC ATGTTGTCCC CTAAAACAGG TGGAAAAGAG 
GCTGCTTCAG GCACCACACC TCAGAAGTCC CGGAAGCCCA AGAAAGGGGC TGGGAACCCC 
CAAGCCTCAA CCCTGGCGCT GCAAAGCAAC ATCGCCCAGT GCCTCCTGGG CCAACCCTGG 
CCCCTGAATG AGGCCCAGGT GCAGGCCTCA GTGGTGAAGG TCCTGACTGA GCTGCTGGAA 
CAGGAAAGAA AGAAGGTGGT GGACACCACC AAGGAGAGCA GCAGGAAGGG CTGGGAGAGC 
CGCAAGCGCA AGCTATCGGG AGACCAGCTG GCTGCCAGGA CCCCCAGGAG CAAGAAGAAG 
AAGAAGCTGG GGGCCAGGGA AGGTGGGGAG GCCTCTGTTT CCCCAGAAAA GACCTCCACG 
ACTTCCAAGG GGAAAGCAAA GAGAGACAAA GCGAGTGGTG ATGTCAAGGA GAAGAAAGGG 
AAGGGGTCTC TTGGCTCCCA AGGGGCCAAG GACGAGCCAG AAGAGGAGCT TCAGAAGGGG 
ATGGGGAAGG TTGAAGGTGG AGATCAAAGC AACCCAAAGA GCAAGAAGGA GAAGAAGAAA 
TCCGACAAGA GAAAAAAAGA CAAAGAAAAA AAAGAAAAGA AGAAGAAAGC AAAAAAGGCC 
TCAACCAAAG ATTCTGAGTC ACCGTCCCAG AAGAAAAAGA AGAAAAAGAA GAAGACAGCA 
GAGCAGACTG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_016954040.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016809529.1 Pan troglodytes treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPc85507
Clone ID Related Accession (Same CDS sequence)	XM_016954040.1
Accession Version	XM_016954040.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4578bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	treacle protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006472.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCAGAGAG CTCGGAAGAG GAGGAGGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCGAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCTTG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGT 
GAGGACACCT CCAGCTCCAG CGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCGCCAGCC 
AAGAAGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CGGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCAT 
GGGAAGGCAG GAGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGTG AGGAGTCGTC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GTGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAAGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG ACTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAGGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCC ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCTGG GAAGGTGGGG CCTGCAACCC CCTCGGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCT 
GCCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CGTCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGTCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCGGC CTCAGTGCCT CTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAGTCAGAC AGTGAGGAGG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGTCAAA 
CAGAGATCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCGGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GTGGAGACGC TGGCTCAGGC GAAGCCTTCA GGGAAGACCC CCCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AAGCACCGGC AGCTGTGACC TCTGCCCAGA AGGACAGTAA CTCCAAACCT 
GCCAGAAGCA AGACCCTGGC CCCAGCACCC CCAGAGAGGA ACACGGAGGG GTCCTCGGAG 
AGCAGTGAGG AAGAGCTGCC ACTGACCCAG GTGATTAAAC CCCCTCTGAT TTTTGTCGAC 
CCTAATCGTA GTCCAGCTGG CCCAGCTGCT ACACCCGCAC AAGCCCAGGC TGCAAGCACC 
CCGAGGAAGG GCCGAGCCTC GGAGAGCACA GCCAGGAGCT CCTCCTCCGA GAGCGAGGAT 
GAGGACGTGA TCCCCGCTAC ACAGTGCTTG ACTCCTGGCA TCAGAACCAA TGTGGTGACC 
ATGCCCACTG CCCACCCAAG AATAGCCCCC AAAGCCAGCA TGGCTGGGGC CAGCAGCAGC 
AAGGAGTCCA GTCGGATATC AGATGGCAAG AAACAGGAGG GACCAGCCAC TCAGGTGTCA 
AAGAAGAACC CAGCTTCCCT CCCACTGACC CAGGCTACCC TGAAGGTCCT CGCCCAGAAA 
GCCAGTGAGG CTCAGCCTCC TGTTGCCAGG ACCCAGCCTT CAAGTGGGGT TGACAGTGCT 
GTGGGAACAC TCCCTGCAAC AAGTCCCCAG AGCAGCTCCG TCCAGGCCAA AGGGACCAAC 
AAGCTCAGAA AACCTAAGCT TCCTGAGGTC CAGCAGGCCA CCAAAGCCCC TGAGAGCTCA 
GATGACAGTG AGGACAGCAG CGACAGTTCT TCAGGGAGTG AGGAAGATGC TGAAGGGCCC 
CAGGGGGCCA AGTCGGCCCA CACGCTGGGT CCCACCCCCT CCAGGACAGA GACCCTGGTG 
GAGGAGACCG CAGCAGAGTC CAGCGAGGAT GATGTGGTGG CGCCATCCCA GTCTCTCCTC 
TCAGGTTATA TGACCCCTGG ACTAACCCCA GCCAATTCCC AGGCCTCAAA AGCCACTCCC 
AAGCTAGACT CCAGCCCCTC AGTTTCCTCT ACTCTGGCCG CCAAAGATGA CCCAGATGGC 
AAGCAGGAGG CAAAGCCCCA ACAGGCAGCA GGCATGTTGT CCCCTAAAAC AGGTGGAAAA 
GAGGCTGCTT CAGGCACCAC ACCTCAGAAG TCCCGGAAGC CCAAGAAAGG GGCTGGGAAC 
CCCCAAGCCT CAACCCTGGC GCTGCAAAGC AACATCGCCC AGTGCCTCCT GGGCCAACCC 
TGGCCCCTGA ATGAGGCCCA GGTGCAGGCC TCAGTGGTGA AGGTCCTGAC TGAGCTGCTG 
GAACAGGAAA GAAAGAAGGT GGTGGACACC ACCAAGGAGA GCAGCAGGAA GGGCTGGGAG 
AGCCGCAAGC GCAAGCTATC GGGAGACCAG CTGGCTGCCA GGACCCCCAG GAGCAAGAAG 
AAGAAGAAGC TGGGGGCCAG GGAAGGTGGG GAGGCCTCTG TTTCCCCAGA AAAGACCTCC 
ACGACTTCCA AGGGGAAAGC AAAGAGAGAC AAAGCGAGTG GTGATGTCAA GGAGAAGAAA 
GGGAAGGGGT CTCTTGGCTC CCAAGGGGCC AAGGACGAGC CAGAAGAGGA GCTTCAGAAG 
GGGATGGGGA AGGTTGAAGG TGGAGATCAA AGCAACCCAA AGAGCAAGAA GGAGAAGAAG 
AAATCCGACA AGAGAAAAAA AGACAAAGAA AAAAAAGAAA AGAAGAAGAA AGCAAAAAAG 
GCCTCAACCA AAGATTCTGA GTCACCGTCC CAGAAGAAAA AGAAGAAAAA GAAGAAGACA 
GCAGAGCAGA CTGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016809529.1
CDS	104..4681
Translation	MAEARKRRELLPLIYHHLLRAGYVRAAREVKEQSGQKCFLAQPVTLLDIYTHWQQTSELGRKRKAEEDAALQAKKTRVSDPISTSESSEEEEEAEAETAKATPRLASTNSSVLGADLPSSMKEKAKAETEKAGKTGNSMPHPATGKTVANLLSGKSPRKSAEPSANTTLVSETEEEGSVLAFGAAAKPGMVSAGQADSSSEDTSSSSDETDVEGKPSVKPAQVKASSVSTKESPARKAAPAPGKVGDVTPQVKGGALPPAKKAKKPEEESESSEEGSESEEEAPAGTRSQVKASEKILQVRAASAPAKGTPGKGATPAPHGKAGAVASQTKAGKPEEDSESSSEESSDSEEETPAAKALLQAKASGKTSQVGAASAPAKESPRKGAAPAPPGKTGPAVAKAQAGKREEDSQSSSEESDSEEEAPAQAKPSGKAPQVRAASAPAKESPRKGTAPAPPRKTGPAAAQVQVGKQEEDSGSSSEESDSDREALAAMNAAQVKPLGKSPQVKPASTMGMGPLGKGAGPVPPGKVGPATPSAQVGKWEEDSESSSEESSDSSDGEVPTAVAPAQEKSLGNILQAKPASSPAKGPPQKAGPVAVQVKAEKPMDNSESSEESSDSADSEEAPAVMTAAQAKPALKIPQTKACPKKTNTTASAKVAPVRVGTQAPRKAGTATSPAGSSPAVAGGTQRPAEDSSSSEESDSEEEKTGLAVTVGQAKSVGKGLQVKAASVPLKGSLGQGTAPVLPGKTGPTVTQVKAEKQEDSESSEEESDSEEAAASPAQVKTSVKKTQAKANPAAARAPSAKGTISAPGKVVTAAAQVKQRSPSKVKPPVRNPQNSTVLARGPASVPSVGKAVATAARAQTGPEEDSGSSEEESDSEEEVETLAQAKPSGKTPQIRAALAPAKESPRKGAAPTPPGKTGPSAAQAGKQDDSGSSSEESDSDGEAPAAVTSAQKDSNSKPARSKTLAPAPPERNTEGSSESSEEELPLTQVIKPPLIFVDPNRSPAGPAATPAQAQAASTPRKGRASESTARSSSSESEDEDVIPATQCLTPGIRTNVVTMPTAHPRIAPKASMAGASSSKESSRISDGKKQEGPATQVSKKNPASLPLTQATLKVLAQKASEAQPPVARTQPSSGVDSAVGTLPATSPQSSSVQAKGTNKLRKPKLPEVQQATKAPESSDDSEDSSDSSSGSEEDAEGPQGAKSAHTLGPTPSRTETLVEETAAESSEDDVVAPSQSLLSGYMTPGLTPANSQASKATPKLDSSPSVSSTLAAKDDPDGKQEAKPQQAAGMLSPKTGGKEAASGTTPQKSRKPKKGAGNPQASTLALQSNIAQCLLGQPWPLNEAQVQASVVKVLTELLEQERKKVVDTTKESSRKGWESRKRKLSGDQLAARTPRSKKKKKLGAREGGEASVSPEKTSTTSKGKAKRDKASGDVKEKKGKGSLGSQGAKDEPEEELQKGMGKVEGGDQSNPKSKKEKKKSDKRKKDKEKKEKKKKAKKASTKDSESPSQKKKKKKKKTAEQTV

Target ORF information:

RefSeq Version	XM_016954040.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016954040.1

ORF Insert Sequence:

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCAGAGAG CTCGGAAGAG GAGGAGGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCGAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCTTG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGT 
GAGGACACCT CCAGCTCCAG CGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCGCCAGCC 
AAGAAGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CGGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCAT 
GGGAAGGCAG GAGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGTG AGGAGTCGTC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GTGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAAGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG ACTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAGGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCC ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCTGG GAAGGTGGGG CCTGCAACCC CCTCGGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCT 
GCCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CGTCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGTCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCGGC CTCAGTGCCT CTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAGTCAGAC AGTGAGGAGG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGTCAAA 
CAGAGATCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCGGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GTGGAGACGC TGGCTCAGGC GAAGCCTTCA GGGAAGACCC CCCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AAGCACCGGC AGCTGTGACC TCTGCCCAGA AGGACAGTAA CTCCAAACCT 
GCCAGAAGCA AGACCCTGGC CCCAGCACCC CCAGAGAGGA ACACGGAGGG GTCCTCGGAG 
AGCAGTGAGG AAGAGCTGCC ACTGACCCAG GTGATTAAAC CCCCTCTGAT TTTTGTCGAC 
CCTAATCGTA GTCCAGCTGG CCCAGCTGCT ACACCCGCAC AAGCCCAGGC TGCAAGCACC 
CCGAGGAAGG GCCGAGCCTC GGAGAGCACA GCCAGGAGCT CCTCCTCCGA GAGCGAGGAT 
GAGGACGTGA TCCCCGCTAC ACAGTGCTTG ACTCCTGGCA TCAGAACCAA TGTGGTGACC 
ATGCCCACTG CCCACCCAAG AATAGCCCCC AAAGCCAGCA TGGCTGGGGC CAGCAGCAGC 
AAGGAGTCCA GTCGGATATC AGATGGCAAG AAACAGGAGG GACCAGCCAC TCAGGTGTCA 
AAGAAGAACC CAGCTTCCCT CCCACTGACC CAGGCTACCC TGAAGGTCCT CGCCCAGAAA 
GCCAGTGAGG CTCAGCCTCC TGTTGCCAGG ACCCAGCCTT CAAGTGGGGT TGACAGTGCT 
GTGGGAACAC TCCCTGCAAC AAGTCCCCAG AGCAGCTCCG TCCAGGCCAA AGGGACCAAC 
AAGCTCAGAA AACCTAAGCT TCCTGAGGTC CAGCAGGCCA CCAAAGCCCC TGAGAGCTCA 
GATGACAGTG AGGACAGCAG CGACAGTTCT TCAGGGAGTG AGGAAGATGC TGAAGGGCCC 
CAGGGGGCCA AGTCGGCCCA CACGCTGGGT CCCACCCCCT CCAGGACAGA GACCCTGGTG 
GAGGAGACCG CAGCAGAGTC CAGCGAGGAT GATGTGGTGG CGCCATCCCA GTCTCTCCTC 
TCAGGTTATA TGACCCCTGG ACTAACCCCA GCCAATTCCC AGGCCTCAAA AGCCACTCCC 
AAGCTAGACT CCAGCCCCTC AGTTTCCTCT ACTCTGGCCG CCAAAGATGA CCCAGATGGC 
AAGCAGGAGG CAAAGCCCCA ACAGGCAGCA GGCATGTTGT CCCCTAAAAC AGGTGGAAAA 
GAGGCTGCTT CAGGCACCAC ACCTCAGAAG TCCCGGAAGC CCAAGAAAGG GGCTGGGAAC 
CCCCAAGCCT CAACCCTGGC GCTGCAAAGC AACATCGCCC AGTGCCTCCT GGGCCAACCC 
TGGCCCCTGA ATGAGGCCCA GGTGCAGGCC TCAGTGGTGA AGGTCCTGAC TGAGCTGCTG 
GAACAGGAAA GAAAGAAGGT GGTGGACACC ACCAAGGAGA GCAGCAGGAA GGGCTGGGAG 
AGCCGCAAGC GCAAGCTATC GGGAGACCAG CTGGCTGCCA GGACCCCCAG GAGCAAGAAG 
AAGAAGAAGC TGGGGGCCAG GGAAGGTGGG GAGGCCTCTG TTTCCCCAGA AAAGACCTCC 
ACGACTTCCA AGGGGAAAGC AAAGAGAGAC AAAGCGAGTG GTGATGTCAA GGAGAAGAAA 
GGGAAGGGGT CTCTTGGCTC CCAAGGGGCC AAGGACGAGC CAGAAGAGGA GCTTCAGAAG 
GGGATGGGGA AGGTTGAAGG TGGAGATCAA AGCAACCCAA AGAGCAAGAA GGAGAAGAAG 
AAATCCGACA AGAGAAAAAA AGACAAAGAA AAAAAAGAAA AGAAGAAGAA AGCAAAAAAG 
GCCTCAACCA AAGATTCTGA GTCACCGTCC CAGAAGAAAA AGAAGAAAAA GAAGAAGACA 
GCAGAGCAGA CTGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_016954041.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016809530.1 Pan troglodytes treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X2, mRNA.

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CloneID	OPc85508
Clone ID Related Accession (Same CDS sequence)	XM_016954041.1
Accession Version	XM_016954041.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4575bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	treacle protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006472.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCAGAGAG CTCGGAAGAG GAGGAGGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCGAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCTTG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGT 
GAGGACACCT CCAGCTCCAG CGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCGCCAGCC 
AAGAAGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CGGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCAT 
GGGAAGGCAG GAGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGTG AGGAGTCGTC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GTGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAAGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG ACTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAGGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCC ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCTGG GAAGGTGGGG CCTGCAACCC CCTCGGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCT 
GCCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CGTCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGTCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCGGC CTCAGTGCCT CTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAGTCAGAC AGTGAGGAGG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGTCAAA 
CAGAGATCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCGGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GTGGAGACGC TGGCTCAGGC GAAGCCTTCA GGGAAGACCC CCCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AAGCACCGGC AGCTGTGACC TCTGCCCAGG ACAGTAACTC CAAACCTGCC 
AGAAGCAAGA CCCTGGCCCC AGCACCCCCA GAGAGGAACA CGGAGGGGTC CTCGGAGAGC 
AGTGAGGAAG AGCTGCCACT GACCCAGGTG ATTAAACCCC CTCTGATTTT TGTCGACCCT 
AATCGTAGTC CAGCTGGCCC AGCTGCTACA CCCGCACAAG CCCAGGCTGC AAGCACCCCG 
AGGAAGGGCC GAGCCTCGGA GAGCACAGCC AGGAGCTCCT CCTCCGAGAG CGAGGATGAG 
GACGTGATCC CCGCTACACA GTGCTTGACT CCTGGCATCA GAACCAATGT GGTGACCATG 
CCCACTGCCC ACCCAAGAAT AGCCCCCAAA GCCAGCATGG CTGGGGCCAG CAGCAGCAAG 
GAGTCCAGTC GGATATCAGA TGGCAAGAAA CAGGAGGGAC CAGCCACTCA GGTGTCAAAG 
AAGAACCCAG CTTCCCTCCC ACTGACCCAG GCTACCCTGA AGGTCCTCGC CCAGAAAGCC 
AGTGAGGCTC AGCCTCCTGT TGCCAGGACC CAGCCTTCAA GTGGGGTTGA CAGTGCTGTG 
GGAACACTCC CTGCAACAAG TCCCCAGAGC AGCTCCGTCC AGGCCAAAGG GACCAACAAG 
CTCAGAAAAC CTAAGCTTCC TGAGGTCCAG CAGGCCACCA AAGCCCCTGA GAGCTCAGAT 
GACAGTGAGG ACAGCAGCGA CAGTTCTTCA GGGAGTGAGG AAGATGCTGA AGGGCCCCAG 
GGGGCCAAGT CGGCCCACAC GCTGGGTCCC ACCCCCTCCA GGACAGAGAC CCTGGTGGAG 
GAGACCGCAG CAGAGTCCAG CGAGGATGAT GTGGTGGCGC CATCCCAGTC TCTCCTCTCA 
GGTTATATGA CCCCTGGACT AACCCCAGCC AATTCCCAGG CCTCAAAAGC CACTCCCAAG 
CTAGACTCCA GCCCCTCAGT TTCCTCTACT CTGGCCGCCA AAGATGACCC AGATGGCAAG 
CAGGAGGCAA AGCCCCAACA GGCAGCAGGC ATGTTGTCCC CTAAAACAGG TGGAAAAGAG 
GCTGCTTCAG GCACCACACC TCAGAAGTCC CGGAAGCCCA AGAAAGGGGC TGGGAACCCC 
CAAGCCTCAA CCCTGGCGCT GCAAAGCAAC ATCGCCCAGT GCCTCCTGGG CCAACCCTGG 
CCCCTGAATG AGGCCCAGGT GCAGGCCTCA GTGGTGAAGG TCCTGACTGA GCTGCTGGAA 
CAGGAAAGAA AGAAGGTGGT GGACACCACC AAGGAGAGCA GCAGGAAGGG CTGGGAGAGC 
CGCAAGCGCA AGCTATCGGG AGACCAGCTG GCTGCCAGGA CCCCCAGGAG CAAGAAGAAG 
AAGAAGCTGG GGGCCAGGGA AGGTGGGGAG GCCTCTGTTT CCCCAGAAAA GACCTCCACG 
ACTTCCAAGG GGAAAGCAAA GAGAGACAAA GCGAGTGGTG ATGTCAAGGA GAAGAAAGGG 
AAGGGGTCTC TTGGCTCCCA AGGGGCCAAG GACGAGCCAG AAGAGGAGCT TCAGAAGGGG 
ATGGGGAAGG TTGAAGGTGG AGATCAAAGC AACCCAAAGA GCAAGAAGGA GAAGAAGAAA 
TCCGACAAGA GAAAAAAAGA CAAAGAAAAA AAAGAAAAGA AGAAGAAAGC AAAAAAGGCC 
TCAACCAAAG ATTCTGAGTC ACCGTCCCAG AAGAAAAAGA AGAAAAAGAA GAAGACAGCA 
GAGCAGACTG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016809530.1
CDS	104..4678
Translation	MAEARKRRELLPLIYHHLLRAGYVRAAREVKEQSGQKCFLAQPVTLLDIYTHWQQTSELGRKRKAEEDAALQAKKTRVSDPISTSESSEEEEEAEAETAKATPRLASTNSSVLGADLPSSMKEKAKAETEKAGKTGNSMPHPATGKTVANLLSGKSPRKSAEPSANTTLVSETEEEGSVLAFGAAAKPGMVSAGQADSSSEDTSSSSDETDVEGKPSVKPAQVKASSVSTKESPARKAAPAPGKVGDVTPQVKGGALPPAKKAKKPEEESESSEEGSESEEEAPAGTRSQVKASEKILQVRAASAPAKGTPGKGATPAPHGKAGAVASQTKAGKPEEDSESSSEESSDSEEETPAAKALLQAKASGKTSQVGAASAPAKESPRKGAAPAPPGKTGPAVAKAQAGKREEDSQSSSEESDSEEEAPAQAKPSGKAPQVRAASAPAKESPRKGTAPAPPRKTGPAAAQVQVGKQEEDSGSSSEESDSDREALAAMNAAQVKPLGKSPQVKPASTMGMGPLGKGAGPVPPGKVGPATPSAQVGKWEEDSESSSEESSDSSDGEVPTAVAPAQEKSLGNILQAKPASSPAKGPPQKAGPVAVQVKAEKPMDNSESSEESSDSADSEEAPAVMTAAQAKPALKIPQTKACPKKTNTTASAKVAPVRVGTQAPRKAGTATSPAGSSPAVAGGTQRPAEDSSSSEESDSEEEKTGLAVTVGQAKSVGKGLQVKAASVPLKGSLGQGTAPVLPGKTGPTVTQVKAEKQEDSESSEEESDSEEAAASPAQVKTSVKKTQAKANPAAARAPSAKGTISAPGKVVTAAAQVKQRSPSKVKPPVRNPQNSTVLARGPASVPSVGKAVATAARAQTGPEEDSGSSEEESDSEEEVETLAQAKPSGKTPQIRAALAPAKESPRKGAAPTPPGKTGPSAAQAGKQDDSGSSSEESDSDGEAPAAVTSAQDSNSKPARSKTLAPAPPERNTEGSSESSEEELPLTQVIKPPLIFVDPNRSPAGPAATPAQAQAASTPRKGRASESTARSSSSESEDEDVIPATQCLTPGIRTNVVTMPTAHPRIAPKASMAGASSSKESSRISDGKKQEGPATQVSKKNPASLPLTQATLKVLAQKASEAQPPVARTQPSSGVDSAVGTLPATSPQSSSVQAKGTNKLRKPKLPEVQQATKAPESSDDSEDSSDSSSGSEEDAEGPQGAKSAHTLGPTPSRTETLVEETAAESSEDDVVAPSQSLLSGYMTPGLTPANSQASKATPKLDSSPSVSSTLAAKDDPDGKQEAKPQQAAGMLSPKTGGKEAASGTTPQKSRKPKKGAGNPQASTLALQSNIAQCLLGQPWPLNEAQVQASVVKVLTELLEQERKKVVDTTKESSRKGWESRKRKLSGDQLAARTPRSKKKKKLGAREGGEASVSPEKTSTTSKGKAKRDKASGDVKEKKGKGSLGSQGAKDEPEEELQKGMGKVEGGDQSNPKSKKEKKKSDKRKKDKEKKEKKKKAKKASTKDSESPSQKKKKKKKKTAEQTV

Target ORF information:

RefSeq Version	XM_016954041.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016954041.1

ORF Insert Sequence:

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCAGAGAG CTCGGAAGAG GAGGAGGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCGAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCTTG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGT 
GAGGACACCT CCAGCTCCAG CGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCGCCAGCC 
AAGAAGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CGGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCAT 
GGGAAGGCAG GAGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGTG AGGAGTCGTC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GTGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAAGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG ACTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAGGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCC ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCTGG GAAGGTGGGG CCTGCAACCC CCTCGGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCT 
GCCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CGTCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGTCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCGGC CTCAGTGCCT CTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAGTCAGAC AGTGAGGAGG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGTCAAA 
CAGAGATCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCGGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GTGGAGACGC TGGCTCAGGC GAAGCCTTCA GGGAAGACCC CCCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AAGCACCGGC AGCTGTGACC TCTGCCCAGG ACAGTAACTC CAAACCTGCC 
AGAAGCAAGA CCCTGGCCCC AGCACCCCCA GAGAGGAACA CGGAGGGGTC CTCGGAGAGC 
AGTGAGGAAG AGCTGCCACT GACCCAGGTG ATTAAACCCC CTCTGATTTT TGTCGACCCT 
AATCGTAGTC CAGCTGGCCC AGCTGCTACA CCCGCACAAG CCCAGGCTGC AAGCACCCCG 
AGGAAGGGCC GAGCCTCGGA GAGCACAGCC AGGAGCTCCT CCTCCGAGAG CGAGGATGAG 
GACGTGATCC CCGCTACACA GTGCTTGACT CCTGGCATCA GAACCAATGT GGTGACCATG 
CCCACTGCCC ACCCAAGAAT AGCCCCCAAA GCCAGCATGG CTGGGGCCAG CAGCAGCAAG 
GAGTCCAGTC GGATATCAGA TGGCAAGAAA CAGGAGGGAC CAGCCACTCA GGTGTCAAAG 
AAGAACCCAG CTTCCCTCCC ACTGACCCAG GCTACCCTGA AGGTCCTCGC CCAGAAAGCC 
AGTGAGGCTC AGCCTCCTGT TGCCAGGACC CAGCCTTCAA GTGGGGTTGA CAGTGCTGTG 
GGAACACTCC CTGCAACAAG TCCCCAGAGC AGCTCCGTCC AGGCCAAAGG GACCAACAAG 
CTCAGAAAAC CTAAGCTTCC TGAGGTCCAG CAGGCCACCA AAGCCCCTGA GAGCTCAGAT 
GACAGTGAGG ACAGCAGCGA CAGTTCTTCA GGGAGTGAGG AAGATGCTGA AGGGCCCCAG 
GGGGCCAAGT CGGCCCACAC GCTGGGTCCC ACCCCCTCCA GGACAGAGAC CCTGGTGGAG 
GAGACCGCAG CAGAGTCCAG CGAGGATGAT GTGGTGGCGC CATCCCAGTC TCTCCTCTCA 
GGTTATATGA CCCCTGGACT AACCCCAGCC AATTCCCAGG CCTCAAAAGC CACTCCCAAG 
CTAGACTCCA GCCCCTCAGT TTCCTCTACT CTGGCCGCCA AAGATGACCC AGATGGCAAG 
CAGGAGGCAA AGCCCCAACA GGCAGCAGGC ATGTTGTCCC CTAAAACAGG TGGAAAAGAG 
GCTGCTTCAG GCACCACACC TCAGAAGTCC CGGAAGCCCA AGAAAGGGGC TGGGAACCCC 
CAAGCCTCAA CCCTGGCGCT GCAAAGCAAC ATCGCCCAGT GCCTCCTGGG CCAACCCTGG 
CCCCTGAATG AGGCCCAGGT GCAGGCCTCA GTGGTGAAGG TCCTGACTGA GCTGCTGGAA 
CAGGAAAGAA AGAAGGTGGT GGACACCACC AAGGAGAGCA GCAGGAAGGG CTGGGAGAGC 
CGCAAGCGCA AGCTATCGGG AGACCAGCTG GCTGCCAGGA CCCCCAGGAG CAAGAAGAAG 
AAGAAGCTGG GGGCCAGGGA AGGTGGGGAG GCCTCTGTTT CCCCAGAAAA GACCTCCACG 
ACTTCCAAGG GGAAAGCAAA GAGAGACAAA GCGAGTGGTG ATGTCAAGGA GAAGAAAGGG 
AAGGGGTCTC TTGGCTCCCA AGGGGCCAAG GACGAGCCAG AAGAGGAGCT TCAGAAGGGG 
ATGGGGAAGG TTGAAGGTGG AGATCAAAGC AACCCAAAGA GCAAGAAGGA GAAGAAGAAA 
TCCGACAAGA GAAAAAAAGA CAAAGAAAAA AAAGAAAAGA AGAAGAAAGC AAAAAAGGCC 
TCAACCAAAG ATTCTGAGTC ACCGTCCCAG AAGAAAAAGA AGAAAAAGAA GAAGACAGCA 
GAGCAGACTG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_016954042.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016809531.1 Pan troglodytes treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X3, mRNA.

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CloneID	OPc85509
Clone ID Related Accession (Same CDS sequence)	XM_016954042.1
Accession Version	XM_016954042.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4467bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	treacle protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006472.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCAGAGAG CTCGGAAGAG GAGGAGGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCGAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCTTG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGT 
GAGGACACCT CCAGCTCCAG CGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCGCCAGCC 
AAGAAGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CGGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCAT 
GGGAAGGCAG GAGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGTG AGGAGTCGTC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GTGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAAGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG ACTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAGGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCC ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCTGG GAAGGTGGGG CCTGCAACCC CCTCGGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCT 
GCCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CGTCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGTCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCGGC CTCAGTGCCT CTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAGTCAGAC AGTGAGGAGG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGTCAAA 
CAGAGATCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCGGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GTGGAGACGC TGGCTCAGGC GAAGCCTTCA GGGAAGACCC CCCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AAGCACCGGC AGCTGTGACC TCTGCCCAGG TGATTAAACC CCCTCTGATT 
TTTGTCGACC CTAATCGTAG TCCAGCTGGC CCAGCTGCTA CACCCGCACA AGCCCAGGCT 
GCAAGCACCC CGAGGAAGGG CCGAGCCTCG GAGAGCACAG CCAGGAGCTC CTCCTCCGAG 
AGCGAGGATG AGGACGTGAT CCCCGCTACA CAGTGCTTGA CTCCTGGCAT CAGAACCAAT 
GTGGTGACCA TGCCCACTGC CCACCCAAGA ATAGCCCCCA AAGCCAGCAT GGCTGGGGCC 
AGCAGCAGCA AGGAGTCCAG TCGGATATCA GATGGCAAGA AACAGGAGGG ACCAGCCACT 
CAGGTGTCAA AGAAGAACCC AGCTTCCCTC CCACTGACCC AGGCTACCCT GAAGGTCCTC 
GCCCAGAAAG CCAGTGAGGC TCAGCCTCCT GTTGCCAGGA CCCAGCCTTC AAGTGGGGTT 
GACAGTGCTG TGGGAACACT CCCTGCAACA AGTCCCCAGA GCAGCTCCGT CCAGGCCAAA 
GGGACCAACA AGCTCAGAAA ACCTAAGCTT CCTGAGGTCC AGCAGGCCAC CAAAGCCCCT 
GAGAGCTCAG ATGACAGTGA GGACAGCAGC GACAGTTCTT CAGGGAGTGA GGAAGATGCT 
GAAGGGCCCC AGGGGGCCAA GTCGGCCCAC ACGCTGGGTC CCACCCCCTC CAGGACAGAG 
ACCCTGGTGG AGGAGACCGC AGCAGAGTCC AGCGAGGATG ATGTGGTGGC GCCATCCCAG 
TCTCTCCTCT CAGGTTATAT GACCCCTGGA CTAACCCCAG CCAATTCCCA GGCCTCAAAA 
GCCACTCCCA AGCTAGACTC CAGCCCCTCA GTTTCCTCTA CTCTGGCCGC CAAAGATGAC 
CCAGATGGCA AGCAGGAGGC AAAGCCCCAA CAGGCAGCAG GCATGTTGTC CCCTAAAACA 
GGTGGAAAAG AGGCTGCTTC AGGCACCACA CCTCAGAAGT CCCGGAAGCC CAAGAAAGGG 
GCTGGGAACC CCCAAGCCTC AACCCTGGCG CTGCAAAGCA ACATCGCCCA GTGCCTCCTG 
GGCCAACCCT GGCCCCTGAA TGAGGCCCAG GTGCAGGCCT CAGTGGTGAA GGTCCTGACT 
GAGCTGCTGG AACAGGAAAG AAAGAAGGTG GTGGACACCA CCAAGGAGAG CAGCAGGAAG 
GGCTGGGAGA GCCGCAAGCG CAAGCTATCG GGAGACCAGC TGGCTGCCAG GACCCCCAGG 
AGCAAGAAGA AGAAGAAGCT GGGGGCCAGG GAAGGTGGGG AGGCCTCTGT TTCCCCAGAA 
AAGACCTCCA CGACTTCCAA GGGGAAAGCA AAGAGAGACA AAGCGAGTGG TGATGTCAAG 
GAGAAGAAAG GGAAGGGGTC TCTTGGCTCC CAAGGGGCCA AGGACGAGCC AGAAGAGGAG 
CTTCAGAAGG GGATGGGGAA GGTTGAAGGT GGAGATCAAA GCAACCCAAA GAGCAAGAAG 
GAGAAGAAGA AATCCGACAA GAGAAAAAAA GACAAAGAAA AAAAAGAAAA GAAGAAGAAA 
GCAAAAAAGG CCTCAACCAA AGATTCTGAG TCACCGTCCC AGAAGAAAAA GAAGAAAAAG 
AAGAAGACAG CAGAGCAGAC TGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016809531.1
CDS	104..4570
Translation	MAEARKRRELLPLIYHHLLRAGYVRAAREVKEQSGQKCFLAQPVTLLDIYTHWQQTSELGRKRKAEEDAALQAKKTRVSDPISTSESSEEEEEAEAETAKATPRLASTNSSVLGADLPSSMKEKAKAETEKAGKTGNSMPHPATGKTVANLLSGKSPRKSAEPSANTTLVSETEEEGSVLAFGAAAKPGMVSAGQADSSSEDTSSSSDETDVEGKPSVKPAQVKASSVSTKESPARKAAPAPGKVGDVTPQVKGGALPPAKKAKKPEEESESSEEGSESEEEAPAGTRSQVKASEKILQVRAASAPAKGTPGKGATPAPHGKAGAVASQTKAGKPEEDSESSSEESSDSEEETPAAKALLQAKASGKTSQVGAASAPAKESPRKGAAPAPPGKTGPAVAKAQAGKREEDSQSSSEESDSEEEAPAQAKPSGKAPQVRAASAPAKESPRKGTAPAPPRKTGPAAAQVQVGKQEEDSGSSSEESDSDREALAAMNAAQVKPLGKSPQVKPASTMGMGPLGKGAGPVPPGKVGPATPSAQVGKWEEDSESSSEESSDSSDGEVPTAVAPAQEKSLGNILQAKPASSPAKGPPQKAGPVAVQVKAEKPMDNSESSEESSDSADSEEAPAVMTAAQAKPALKIPQTKACPKKTNTTASAKVAPVRVGTQAPRKAGTATSPAGSSPAVAGGTQRPAEDSSSSEESDSEEEKTGLAVTVGQAKSVGKGLQVKAASVPLKGSLGQGTAPVLPGKTGPTVTQVKAEKQEDSESSEEESDSEEAAASPAQVKTSVKKTQAKANPAAARAPSAKGTISAPGKVVTAAAQVKQRSPSKVKPPVRNPQNSTVLARGPASVPSVGKAVATAARAQTGPEEDSGSSEEESDSEEEVETLAQAKPSGKTPQIRAALAPAKESPRKGAAPTPPGKTGPSAAQAGKQDDSGSSSEESDSDGEAPAAVTSAQVIKPPLIFVDPNRSPAGPAATPAQAQAASTPRKGRASESTARSSSSESEDEDVIPATQCLTPGIRTNVVTMPTAHPRIAPKASMAGASSSKESSRISDGKKQEGPATQVSKKNPASLPLTQATLKVLAQKASEAQPPVARTQPSSGVDSAVGTLPATSPQSSSVQAKGTNKLRKPKLPEVQQATKAPESSDDSEDSSDSSSGSEEDAEGPQGAKSAHTLGPTPSRTETLVEETAAESSEDDVVAPSQSLLSGYMTPGLTPANSQASKATPKLDSSPSVSSTLAAKDDPDGKQEAKPQQAAGMLSPKTGGKEAASGTTPQKSRKPKKGAGNPQASTLALQSNIAQCLLGQPWPLNEAQVQASVVKVLTELLEQERKKVVDTTKESSRKGWESRKRKLSGDQLAARTPRSKKKKKLGAREGGEASVSPEKTSTTSKGKAKRDKASGDVKEKKGKGSLGSQGAKDEPEEELQKGMGKVEGGDQSNPKSKKEKKKSDKRKKDKEKKEKKKKAKKASTKDSESPSQKKKKKKKKTAEQTV

Target ORF information:

RefSeq Version	XM_016954042.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016954042.1

ORF Insert Sequence:

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCAGAGAG CTCGGAAGAG GAGGAGGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCGAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCTTG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGT 
GAGGACACCT CCAGCTCCAG CGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCGCCAGCC 
AAGAAGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CGGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCAT 
GGGAAGGCAG GAGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGTG AGGAGTCGTC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GTGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAAGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG ACTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAGGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCC ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCTGG GAAGGTGGGG CCTGCAACCC CCTCGGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCT 
GCCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CGTCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGTCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCGGC CTCAGTGCCT CTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAGTCAGAC AGTGAGGAGG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGTCAAA 
CAGAGATCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCGGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GTGGAGACGC TGGCTCAGGC GAAGCCTTCA GGGAAGACCC CCCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AAGCACCGGC AGCTGTGACC TCTGCCCAGG TGATTAAACC CCCTCTGATT 
TTTGTCGACC CTAATCGTAG TCCAGCTGGC CCAGCTGCTA CACCCGCACA AGCCCAGGCT 
GCAAGCACCC CGAGGAAGGG CCGAGCCTCG GAGAGCACAG CCAGGAGCTC CTCCTCCGAG 
AGCGAGGATG AGGACGTGAT CCCCGCTACA CAGTGCTTGA CTCCTGGCAT CAGAACCAAT 
GTGGTGACCA TGCCCACTGC CCACCCAAGA ATAGCCCCCA AAGCCAGCAT GGCTGGGGCC 
AGCAGCAGCA AGGAGTCCAG TCGGATATCA GATGGCAAGA AACAGGAGGG ACCAGCCACT 
CAGGTGTCAA AGAAGAACCC AGCTTCCCTC CCACTGACCC AGGCTACCCT GAAGGTCCTC 
GCCCAGAAAG CCAGTGAGGC TCAGCCTCCT GTTGCCAGGA CCCAGCCTTC AAGTGGGGTT 
GACAGTGCTG TGGGAACACT CCCTGCAACA AGTCCCCAGA GCAGCTCCGT CCAGGCCAAA 
GGGACCAACA AGCTCAGAAA ACCTAAGCTT CCTGAGGTCC AGCAGGCCAC CAAAGCCCCT 
GAGAGCTCAG ATGACAGTGA GGACAGCAGC GACAGTTCTT CAGGGAGTGA GGAAGATGCT 
GAAGGGCCCC AGGGGGCCAA GTCGGCCCAC ACGCTGGGTC CCACCCCCTC CAGGACAGAG 
ACCCTGGTGG AGGAGACCGC AGCAGAGTCC AGCGAGGATG ATGTGGTGGC GCCATCCCAG 
TCTCTCCTCT CAGGTTATAT GACCCCTGGA CTAACCCCAG CCAATTCCCA GGCCTCAAAA 
GCCACTCCCA AGCTAGACTC CAGCCCCTCA GTTTCCTCTA CTCTGGCCGC CAAAGATGAC 
CCAGATGGCA AGCAGGAGGC AAAGCCCCAA CAGGCAGCAG GCATGTTGTC CCCTAAAACA 
GGTGGAAAAG AGGCTGCTTC AGGCACCACA CCTCAGAAGT CCCGGAAGCC CAAGAAAGGG 
GCTGGGAACC CCCAAGCCTC AACCCTGGCG CTGCAAAGCA ACATCGCCCA GTGCCTCCTG 
GGCCAACCCT GGCCCCTGAA TGAGGCCCAG GTGCAGGCCT CAGTGGTGAA GGTCCTGACT 
GAGCTGCTGG AACAGGAAAG AAAGAAGGTG GTGGACACCA CCAAGGAGAG CAGCAGGAAG 
GGCTGGGAGA GCCGCAAGCG CAAGCTATCG GGAGACCAGC TGGCTGCCAG GACCCCCAGG 
AGCAAGAAGA AGAAGAAGCT GGGGGCCAGG GAAGGTGGGG AGGCCTCTGT TTCCCCAGAA 
AAGACCTCCA CGACTTCCAA GGGGAAAGCA AAGAGAGACA AAGCGAGTGG TGATGTCAAG 
GAGAAGAAAG GGAAGGGGTC TCTTGGCTCC CAAGGGGCCA AGGACGAGCC AGAAGAGGAG 
CTTCAGAAGG GGATGGGGAA GGTTGAAGGT GGAGATCAAA GCAACCCAAA GAGCAAGAAG 
GAGAAGAAGA AATCCGACAA GAGAAAAAAA GACAAAGAAA AAAAAGAAAA GAAGAAGAAA 
GCAAAAAAGG CCTCAACCAA AGATTCTGAG TCACCGTCCC AGAAGAAAAA GAAGAAAAAG 
AAGAAGACAG CAGAGCAGAC TGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_016954043.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016809532.1 Pan troglodytes treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OPc85510
Clone ID Related Accession (Same CDS sequence)	XM_016954043.1
Accession Version	XM_016954043.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4464bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	treacle protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006472.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCAGAGAG CTCGGAAGAG GAGGAGGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCGAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCTTG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGT 
GAGGACACCT CCAGCTCCAG CGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCGCCAGCC 
AAGAAGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CGGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCAT 
GGGAAGGCAG GAGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGTG AGGAGTCGTC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GTGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAAGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG ACTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAGGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCC ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCTGG GAAGGTGGGG CCTGCAACCC CCTCGGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCT 
GCCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CGTCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGTCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCGGC CTCAGTGCCT CTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAGTCAGAC AGTGAGGAGG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGTCAAA 
CAGAGATCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCGGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GTGGAGACGC TGGCTCAGGC GAAGCCTTCA GGGAAGACCC CCCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AAGCACCGGC AGCTGTGACC TCTGCCCAGA AGGACAGTAA CTCCAAACCT 
GCCAGAAGCA AGACCCTGGC CCCAGCACCC CCAGAGAGGA ACACGGAGGG GTCCTCGGAG 
AGCAGTGAGG AAGAGCTGCC ACTGACCCAG GTGATTAAAC CCCCTCTGAT TTTTGTCGAC 
CCTAATCGTA GTCCAGCTGG CCCAGCTGCT ACACCCGCAC AAGCCCAGGC TGCAAGCACC 
CCGAGGAAGG GCCGAGCCTC GGAGAGCACA GCCAGGAGCT CCTCCTCCGA GAGCGAGGAT 
GAGGACGTGA TCCCCGCTAC ACAGTGCTTG ACTCCTGGCA TCAGAACCAA TGTGGTGACC 
ATGCCCACTG CCCACCCAAG AATAGCCCCC AAAGCCAGCA TGGCTGGGGC CAGCAGCAGC 
AAGGAGTCCA GTCGGATATC AGATGGCAAG AAACAGGAGG GACCAGCCAC TCAGGTTGAC 
AGTGCTGTGG GAACACTCCC TGCAACAAGT CCCCAGAGCA GCTCCGTCCA GGCCAAAGGG 
ACCAACAAGC TCAGAAAACC TAAGCTTCCT GAGGTCCAGC AGGCCACCAA AGCCCCTGAG 
AGCTCAGATG ACAGTGAGGA CAGCAGCGAC AGTTCTTCAG GGAGTGAGGA AGATGCTGAA 
GGGCCCCAGG GGGCCAAGTC GGCCCACACG CTGGGTCCCA CCCCCTCCAG GACAGAGACC 
CTGGTGGAGG AGACCGCAGC AGAGTCCAGC GAGGATGATG TGGTGGCGCC ATCCCAGTCT 
CTCCTCTCAG GTTATATGAC CCCTGGACTA ACCCCAGCCA ATTCCCAGGC CTCAAAAGCC 
ACTCCCAAGC TAGACTCCAG CCCCTCAGTT TCCTCTACTC TGGCCGCCAA AGATGACCCA 
GATGGCAAGC AGGAGGCAAA GCCCCAACAG GCAGCAGGCA TGTTGTCCCC TAAAACAGGT 
GGAAAAGAGG CTGCTTCAGG CACCACACCT CAGAAGTCCC GGAAGCCCAA GAAAGGGGCT 
GGGAACCCCC AAGCCTCAAC CCTGGCGCTG CAAAGCAACA TCGCCCAGTG CCTCCTGGGC 
CAACCCTGGC CCCTGAATGA GGCCCAGGTG CAGGCCTCAG TGGTGAAGGT CCTGACTGAG 
CTGCTGGAAC AGGAAAGAAA GAAGGTGGTG GACACCACCA AGGAGAGCAG CAGGAAGGGC 
TGGGAGAGCC GCAAGCGCAA GCTATCGGGA GACCAGCTGG CTGCCAGGAC CCCCAGGAGC 
AAGAAGAAGA AGAAGCTGGG GGCCAGGGAA GGTGGGGAGG CCTCTGTTTC CCCAGAAAAG 
ACCTCCACGA CTTCCAAGGG GAAAGCAAAG AGAGACAAAG CGAGTGGTGA TGTCAAGGAG 
AAGAAAGGGA AGGGGTCTCT TGGCTCCCAA GGGGCCAAGG ACGAGCCAGA AGAGGAGCTT 
CAGAAGGGGA TGGGGAAGGT TGAAGGTGGA GATCAAAGCA ACCCAAAGAG CAAGAAGGAG 
AAGAAGAAAT CCGACAAGAG AAAAAAAGAC AAAGAAAAAA AAGAAAAGAA GAAGAAAGCA 
AAAAAGGCCT CAACCAAAGA TTCTGAGTCA CCGTCCCAGA AGAAAAAGAA GAAAAAGAAG 
AAGACAGCAG AGCAGACTGT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016809532.1
CDS	104..4567
Translation	MAEARKRRELLPLIYHHLLRAGYVRAAREVKEQSGQKCFLAQPVTLLDIYTHWQQTSELGRKRKAEEDAALQAKKTRVSDPISTSESSEEEEEAEAETAKATPRLASTNSSVLGADLPSSMKEKAKAETEKAGKTGNSMPHPATGKTVANLLSGKSPRKSAEPSANTTLVSETEEEGSVLAFGAAAKPGMVSAGQADSSSEDTSSSSDETDVEGKPSVKPAQVKASSVSTKESPARKAAPAPGKVGDVTPQVKGGALPPAKKAKKPEEESESSEEGSESEEEAPAGTRSQVKASEKILQVRAASAPAKGTPGKGATPAPHGKAGAVASQTKAGKPEEDSESSSEESSDSEEETPAAKALLQAKASGKTSQVGAASAPAKESPRKGAAPAPPGKTGPAVAKAQAGKREEDSQSSSEESDSEEEAPAQAKPSGKAPQVRAASAPAKESPRKGTAPAPPRKTGPAAAQVQVGKQEEDSGSSSEESDSDREALAAMNAAQVKPLGKSPQVKPASTMGMGPLGKGAGPVPPGKVGPATPSAQVGKWEEDSESSSEESSDSSDGEVPTAVAPAQEKSLGNILQAKPASSPAKGPPQKAGPVAVQVKAEKPMDNSESSEESSDSADSEEAPAVMTAAQAKPALKIPQTKACPKKTNTTASAKVAPVRVGTQAPRKAGTATSPAGSSPAVAGGTQRPAEDSSSSEESDSEEEKTGLAVTVGQAKSVGKGLQVKAASVPLKGSLGQGTAPVLPGKTGPTVTQVKAEKQEDSESSEEESDSEEAAASPAQVKTSVKKTQAKANPAAARAPSAKGTISAPGKVVTAAAQVKQRSPSKVKPPVRNPQNSTVLARGPASVPSVGKAVATAARAQTGPEEDSGSSEEESDSEEEVETLAQAKPSGKTPQIRAALAPAKESPRKGAAPTPPGKTGPSAAQAGKQDDSGSSSEESDSDGEAPAAVTSAQKDSNSKPARSKTLAPAPPERNTEGSSESSEEELPLTQVIKPPLIFVDPNRSPAGPAATPAQAQAASTPRKGRASESTARSSSSESEDEDVIPATQCLTPGIRTNVVTMPTAHPRIAPKASMAGASSSKESSRISDGKKQEGPATQVDSAVGTLPATSPQSSSVQAKGTNKLRKPKLPEVQQATKAPESSDDSEDSSDSSSGSEEDAEGPQGAKSAHTLGPTPSRTETLVEETAAESSEDDVVAPSQSLLSGYMTPGLTPANSQASKATPKLDSSPSVSSTLAAKDDPDGKQEAKPQQAAGMLSPKTGGKEAASGTTPQKSRKPKKGAGNPQASTLALQSNIAQCLLGQPWPLNEAQVQASVVKVLTELLEQERKKVVDTTKESSRKGWESRKRKLSGDQLAARTPRSKKKKKLGAREGGEASVSPEKTSTTSKGKAKRDKASGDVKEKKGKGSLGSQGAKDEPEEELQKGMGKVEGGDQSNPKSKKEKKKSDKRKKDKEKKEKKKKAKKASTKDSESPSQKKKKKKKKTAEQTV

Target ORF information:

RefSeq Version	XM_016954043.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016954043.1

ORF Insert Sequence:

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCAGAGAG CTCGGAAGAG GAGGAGGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCGAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCTTG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGT 
GAGGACACCT CCAGCTCCAG CGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCGCCAGCC 
AAGAAGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CGGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCAT 
GGGAAGGCAG GAGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGTG AGGAGTCGTC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GTGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAAGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG ACTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAGGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCC ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCTGG GAAGGTGGGG CCTGCAACCC CCTCGGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCT 
GCCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CGTCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGTCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCGGC CTCAGTGCCT CTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAGTCAGAC AGTGAGGAGG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGTCAAA 
CAGAGATCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCGGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GTGGAGACGC TGGCTCAGGC GAAGCCTTCA GGGAAGACCC CCCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AAGCACCGGC AGCTGTGACC TCTGCCCAGA AGGACAGTAA CTCCAAACCT 
GCCAGAAGCA AGACCCTGGC CCCAGCACCC CCAGAGAGGA ACACGGAGGG GTCCTCGGAG 
AGCAGTGAGG AAGAGCTGCC ACTGACCCAG GTGATTAAAC CCCCTCTGAT TTTTGTCGAC 
CCTAATCGTA GTCCAGCTGG CCCAGCTGCT ACACCCGCAC AAGCCCAGGC TGCAAGCACC 
CCGAGGAAGG GCCGAGCCTC GGAGAGCACA GCCAGGAGCT CCTCCTCCGA GAGCGAGGAT 
GAGGACGTGA TCCCCGCTAC ACAGTGCTTG ACTCCTGGCA TCAGAACCAA TGTGGTGACC 
ATGCCCACTG CCCACCCAAG AATAGCCCCC AAAGCCAGCA TGGCTGGGGC CAGCAGCAGC 
AAGGAGTCCA GTCGGATATC AGATGGCAAG AAACAGGAGG GACCAGCCAC TCAGGTTGAC 
AGTGCTGTGG GAACACTCCC TGCAACAAGT CCCCAGAGCA GCTCCGTCCA GGCCAAAGGG 
ACCAACAAGC TCAGAAAACC TAAGCTTCCT GAGGTCCAGC AGGCCACCAA AGCCCCTGAG 
AGCTCAGATG ACAGTGAGGA CAGCAGCGAC AGTTCTTCAG GGAGTGAGGA AGATGCTGAA 
GGGCCCCAGG GGGCCAAGTC GGCCCACACG CTGGGTCCCA CCCCCTCCAG GACAGAGACC 
CTGGTGGAGG AGACCGCAGC AGAGTCCAGC GAGGATGATG TGGTGGCGCC ATCCCAGTCT 
CTCCTCTCAG GTTATATGAC CCCTGGACTA ACCCCAGCCA ATTCCCAGGC CTCAAAAGCC 
ACTCCCAAGC TAGACTCCAG CCCCTCAGTT TCCTCTACTC TGGCCGCCAA AGATGACCCA 
GATGGCAAGC AGGAGGCAAA GCCCCAACAG GCAGCAGGCA TGTTGTCCCC TAAAACAGGT 
GGAAAAGAGG CTGCTTCAGG CACCACACCT CAGAAGTCCC GGAAGCCCAA GAAAGGGGCT 
GGGAACCCCC AAGCCTCAAC CCTGGCGCTG CAAAGCAACA TCGCCCAGTG CCTCCTGGGC 
CAACCCTGGC CCCTGAATGA GGCCCAGGTG CAGGCCTCAG TGGTGAAGGT CCTGACTGAG 
CTGCTGGAAC AGGAAAGAAA GAAGGTGGTG GACACCACCA AGGAGAGCAG CAGGAAGGGC 
TGGGAGAGCC GCAAGCGCAA GCTATCGGGA GACCAGCTGG CTGCCAGGAC CCCCAGGAGC 
AAGAAGAAGA AGAAGCTGGG GGCCAGGGAA GGTGGGGAGG CCTCTGTTTC CCCAGAAAAG 
ACCTCCACGA CTTCCAAGGG GAAAGCAAAG AGAGACAAAG CGAGTGGTGA TGTCAAGGAG 
AAGAAAGGGA AGGGGTCTCT TGGCTCCCAA GGGGCCAAGG ACGAGCCAGA AGAGGAGCTT 
CAGAAGGGGA TGGGGAAGGT TGAAGGTGGA GATCAAAGCA ACCCAAAGAG CAAGAAGGAG 
AAGAAGAAAT CCGACAAGAG AAAAAAAGAC AAAGAAAAAA AAGAAAAGAA GAAGAAAGCA 
AAAAAGGCCT CAACCAAAGA TTCTGAGTCA CCGTCCCAGA AGAAAAAGAA GAAAAAGAAG 
AAGACAGCAG AGCAGACTGT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_016954044.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016809533.1 Pan troglodytes treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OPc85511
Clone ID Related Accession (Same CDS sequence)	XM_016954044.1
Accession Version	XM_016954044.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4398bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	treacle protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006472.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCAGAGAG CTCGGAAGAG GAGGAGGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCGAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCTTG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGT 
GAGGACACCT CCAGCTCCAG CGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCGCCAGCC 
AAGAAGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CGGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCAT 
GGGAAGGCAG GAGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGTG AGGAGTCGTC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GTGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAAGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG ACTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAGGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCC ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCTGG GAAGGTGGGG CCTGCAACCC CCTCGGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCT 
GCCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CGTCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGTCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCGGC CTCAGTGCCT CTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAGTCAGAC AGTGAGGAGG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGTCAAA 
CAGAGATCTC CATCCAAGGC GAAGCCTTCA GGGAAGACCC CCCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AAGCACCGGC AGCTGTGACC TCTGCCCAGA AGGACAGTAA CTCCAAACCT 
GCCAGAAGCA AGACCCTGGC CCCAGCACCC CCAGAGAGGA ACACGGAGGG GTCCTCGGAG 
AGCAGTGAGG AAGAGCTGCC ACTGACCCAG GTGATTAAAC CCCCTCTGAT TTTTGTCGAC 
CCTAATCGTA GTCCAGCTGG CCCAGCTGCT ACACCCGCAC AAGCCCAGGC TGCAAGCACC 
CCGAGGAAGG GCCGAGCCTC GGAGAGCACA GCCAGGAGCT CCTCCTCCGA GAGCGAGGAT 
GAGGACGTGA TCCCCGCTAC ACAGTGCTTG ACTCCTGGCA TCAGAACCAA TGTGGTGACC 
ATGCCCACTG CCCACCCAAG AATAGCCCCC AAAGCCAGCA TGGCTGGGGC CAGCAGCAGC 
AAGGAGTCCA GTCGGATATC AGATGGCAAG AAACAGGAGG GACCAGCCAC TCAGGTGTCA 
AAGAAGAACC CAGCTTCCCT CCCACTGACC CAGGCTACCC TGAAGGTCCT CGCCCAGAAA 
GCCAGTGAGG CTCAGCCTCC TGTTGCCAGG ACCCAGCCTT CAAGTGGGGT TGACAGTGCT 
GTGGGAACAC TCCCTGCAAC AAGTCCCCAG AGCAGCTCCG TCCAGGCCAA AGGGACCAAC 
AAGCTCAGAA AACCTAAGCT TCCTGAGGTC CAGCAGGCCA CCAAAGCCCC TGAGAGCTCA 
GATGACAGTG AGGACAGCAG CGACAGTTCT TCAGGGAGTG AGGAAGATGC TGAAGGGCCC 
CAGGGGGCCA AGTCGGCCCA CACGCTGGGT CCCACCCCCT CCAGGACAGA GACCCTGGTG 
GAGGAGACCG CAGCAGAGTC CAGCGAGGAT GATGTGGTGG CGCCATCCCA GTCTCTCCTC 
TCAGGTTATA TGACCCCTGG ACTAACCCCA GCCAATTCCC AGGCCTCAAA AGCCACTCCC 
AAGCTAGACT CCAGCCCCTC AGTTTCCTCT ACTCTGGCCG CCAAAGATGA CCCAGATGGC 
AAGCAGGAGG CAAAGCCCCA ACAGGCAGCA GGCATGTTGT CCCCTAAAAC AGGTGGAAAA 
GAGGCTGCTT CAGGCACCAC ACCTCAGAAG TCCCGGAAGC CCAAGAAAGG GGCTGGGAAC 
CCCCAAGCCT CAACCCTGGC GCTGCAAAGC AACATCGCCC AGTGCCTCCT GGGCCAACCC 
TGGCCCCTGA ATGAGGCCCA GGTGCAGGCC TCAGTGGTGA AGGTCCTGAC TGAGCTGCTG 
GAACAGGAAA GAAAGAAGGT GGTGGACACC ACCAAGGAGA GCAGCAGGAA GGGCTGGGAG 
AGCCGCAAGC GCAAGCTATC GGGAGACCAG CTGGCTGCCA GGACCCCCAG GAGCAAGAAG 
AAGAAGAAGC TGGGGGCCAG GGAAGGTGGG GAGGCCTCTG TTTCCCCAGA AAAGACCTCC 
ACGACTTCCA AGGGGAAAGC AAAGAGAGAC AAAGCGAGTG GTGATGTCAA GGAGAAGAAA 
GGGAAGGGGT CTCTTGGCTC CCAAGGGGCC AAGGACGAGC CAGAAGAGGA GCTTCAGAAG 
GGGATGGGGA AGGTTGAAGG TGGAGATCAA AGCAACCCAA AGAGCAAGAA GGAGAAGAAG 
AAATCCGACA AGAGAAAAAA AGACAAAGAA AAAAAAGAAA AGAAGAAGAA AGCAAAAAAG 
GCCTCAACCA AAGATTCTGA GTCACCGTCC CAGAAGAAAA AGAAGAAAAA GAAGAAGACA 
GCAGAGCAGA CTGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016809533.1
CDS	104..4501
Translation	MAEARKRRELLPLIYHHLLRAGYVRAAREVKEQSGQKCFLAQPVTLLDIYTHWQQTSELGRKRKAEEDAALQAKKTRVSDPISTSESSEEEEEAEAETAKATPRLASTNSSVLGADLPSSMKEKAKAETEKAGKTGNSMPHPATGKTVANLLSGKSPRKSAEPSANTTLVSETEEEGSVLAFGAAAKPGMVSAGQADSSSEDTSSSSDETDVEGKPSVKPAQVKASSVSTKESPARKAAPAPGKVGDVTPQVKGGALPPAKKAKKPEEESESSEEGSESEEEAPAGTRSQVKASEKILQVRAASAPAKGTPGKGATPAPHGKAGAVASQTKAGKPEEDSESSSEESSDSEEETPAAKALLQAKASGKTSQVGAASAPAKESPRKGAAPAPPGKTGPAVAKAQAGKREEDSQSSSEESDSEEEAPAQAKPSGKAPQVRAASAPAKESPRKGTAPAPPRKTGPAAAQVQVGKQEEDSGSSSEESDSDREALAAMNAAQVKPLGKSPQVKPASTMGMGPLGKGAGPVPPGKVGPATPSAQVGKWEEDSESSSEESSDSSDGEVPTAVAPAQEKSLGNILQAKPASSPAKGPPQKAGPVAVQVKAEKPMDNSESSEESSDSADSEEAPAVMTAAQAKPALKIPQTKACPKKTNTTASAKVAPVRVGTQAPRKAGTATSPAGSSPAVAGGTQRPAEDSSSSEESDSEEEKTGLAVTVGQAKSVGKGLQVKAASVPLKGSLGQGTAPVLPGKTGPTVTQVKAEKQEDSESSEEESDSEEAAASPAQVKTSVKKTQAKANPAAARAPSAKGTISAPGKVVTAAAQVKQRSPSKAKPSGKTPQIRAALAPAKESPRKGAAPTPPGKTGPSAAQAGKQDDSGSSSEESDSDGEAPAAVTSAQKDSNSKPARSKTLAPAPPERNTEGSSESSEEELPLTQVIKPPLIFVDPNRSPAGPAATPAQAQAASTPRKGRASESTARSSSSESEDEDVIPATQCLTPGIRTNVVTMPTAHPRIAPKASMAGASSSKESSRISDGKKQEGPATQVSKKNPASLPLTQATLKVLAQKASEAQPPVARTQPSSGVDSAVGTLPATSPQSSSVQAKGTNKLRKPKLPEVQQATKAPESSDDSEDSSDSSSGSEEDAEGPQGAKSAHTLGPTPSRTETLVEETAAESSEDDVVAPSQSLLSGYMTPGLTPANSQASKATPKLDSSPSVSSTLAAKDDPDGKQEAKPQQAAGMLSPKTGGKEAASGTTPQKSRKPKKGAGNPQASTLALQSNIAQCLLGQPWPLNEAQVQASVVKVLTELLEQERKKVVDTTKESSRKGWESRKRKLSGDQLAARTPRSKKKKKLGAREGGEASVSPEKTSTTSKGKAKRDKASGDVKEKKGKGSLGSQGAKDEPEEELQKGMGKVEGGDQSNPKSKKEKKKSDKRKKDKEKKEKKKKAKKASTKDSESPSQKKKKKKKKTAEQTV

Target ORF information:

RefSeq Version	XM_016954044.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016954044.1

ORF Insert Sequence:

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCAGAGAG CTCGGAAGAG GAGGAGGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCGAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCTTG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGT 
GAGGACACCT CCAGCTCCAG CGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCGCCAGCC 
AAGAAGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CGGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCAT 
GGGAAGGCAG GAGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGTG AGGAGTCGTC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GTGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAAGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG ACTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAGGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCC ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCTGG GAAGGTGGGG CCTGCAACCC CCTCGGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCT 
GCCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CGTCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGTCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCGGC CTCAGTGCCT CTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAGTCAGAC AGTGAGGAGG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGTCAAA 
CAGAGATCTC CATCCAAGGC GAAGCCTTCA GGGAAGACCC CCCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AAGCACCGGC AGCTGTGACC TCTGCCCAGA AGGACAGTAA CTCCAAACCT 
GCCAGAAGCA AGACCCTGGC CCCAGCACCC CCAGAGAGGA ACACGGAGGG GTCCTCGGAG 
AGCAGTGAGG AAGAGCTGCC ACTGACCCAG GTGATTAAAC CCCCTCTGAT TTTTGTCGAC 
CCTAATCGTA GTCCAGCTGG CCCAGCTGCT ACACCCGCAC AAGCCCAGGC TGCAAGCACC 
CCGAGGAAGG GCCGAGCCTC GGAGAGCACA GCCAGGAGCT CCTCCTCCGA GAGCGAGGAT 
GAGGACGTGA TCCCCGCTAC ACAGTGCTTG ACTCCTGGCA TCAGAACCAA TGTGGTGACC 
ATGCCCACTG CCCACCCAAG AATAGCCCCC AAAGCCAGCA TGGCTGGGGC CAGCAGCAGC 
AAGGAGTCCA GTCGGATATC AGATGGCAAG AAACAGGAGG GACCAGCCAC TCAGGTGTCA 
AAGAAGAACC CAGCTTCCCT CCCACTGACC CAGGCTACCC TGAAGGTCCT CGCCCAGAAA 
GCCAGTGAGG CTCAGCCTCC TGTTGCCAGG ACCCAGCCTT CAAGTGGGGT TGACAGTGCT 
GTGGGAACAC TCCCTGCAAC AAGTCCCCAG AGCAGCTCCG TCCAGGCCAA AGGGACCAAC 
AAGCTCAGAA AACCTAAGCT TCCTGAGGTC CAGCAGGCCA CCAAAGCCCC TGAGAGCTCA 
GATGACAGTG AGGACAGCAG CGACAGTTCT TCAGGGAGTG AGGAAGATGC TGAAGGGCCC 
CAGGGGGCCA AGTCGGCCCA CACGCTGGGT CCCACCCCCT CCAGGACAGA GACCCTGGTG 
GAGGAGACCG CAGCAGAGTC CAGCGAGGAT GATGTGGTGG CGCCATCCCA GTCTCTCCTC 
TCAGGTTATA TGACCCCTGG ACTAACCCCA GCCAATTCCC AGGCCTCAAA AGCCACTCCC 
AAGCTAGACT CCAGCCCCTC AGTTTCCTCT ACTCTGGCCG CCAAAGATGA CCCAGATGGC 
AAGCAGGAGG CAAAGCCCCA ACAGGCAGCA GGCATGTTGT CCCCTAAAAC AGGTGGAAAA 
GAGGCTGCTT CAGGCACCAC ACCTCAGAAG TCCCGGAAGC CCAAGAAAGG GGCTGGGAAC 
CCCCAAGCCT CAACCCTGGC GCTGCAAAGC AACATCGCCC AGTGCCTCCT GGGCCAACCC 
TGGCCCCTGA ATGAGGCCCA GGTGCAGGCC TCAGTGGTGA AGGTCCTGAC TGAGCTGCTG 
GAACAGGAAA GAAAGAAGGT GGTGGACACC ACCAAGGAGA GCAGCAGGAA GGGCTGGGAG 
AGCCGCAAGC GCAAGCTATC GGGAGACCAG CTGGCTGCCA GGACCCCCAG GAGCAAGAAG 
AAGAAGAAGC TGGGGGCCAG GGAAGGTGGG GAGGCCTCTG TTTCCCCAGA AAAGACCTCC 
ACGACTTCCA AGGGGAAAGC AAAGAGAGAC AAAGCGAGTG GTGATGTCAA GGAGAAGAAA 
GGGAAGGGGT CTCTTGGCTC CCAAGGGGCC AAGGACGAGC CAGAAGAGGA GCTTCAGAAG 
GGGATGGGGA AGGTTGAAGG TGGAGATCAA AGCAACCCAA AGAGCAAGAA GGAGAAGAAG 
AAATCCGACA AGAGAAAAAA AGACAAAGAA AAAAAAGAAA AGAAGAAGAA AGCAAAAAAG 
GCCTCAACCA AAGATTCTGA GTCACCGTCC CAGAAGAAAA AGAAGAAAAA GAAGAAGACA 
GCAGAGCAGA CTGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_016954046.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016809535.1 Pan troglodytes treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X6, mRNA.

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CloneID	OPc85512
Clone ID Related Accession (Same CDS sequence)	XM_016954046.1
Accession Version	XM_016954046.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4353bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	treacle protein isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006472.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCAGAGAG CTCGGAAGAG GAGGAGGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCGAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCTTG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGT 
GAGGACACCT CCAGCTCCAG CGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCGCCAGCC 
AAGAAGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CGGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCAT 
GGGAAGGCAG GAGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGTG AGGAGTCGTC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GTGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAAGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG ACTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAGGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCC ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCTGG GAAGGTGGGG CCTGCAACCC CCTCGGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCT 
GCCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CGTCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGTCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCGGC CTCAGTGCCT CTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAGTCAGAC AGTGAGGAGG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGTCAAA 
CAGAGATCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCGGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GTGGAGACGC TGGCTCAGGC GAAGCCTTCA GGGAAGACCC CCCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AAGCACCGGC AGCTGTGACC TCTGCCCAGG TGATTAAACC CCCTCTGATT 
TTTGTCGACC CTAATCGTAG TCCAGCTGGC CCAGCTGCTA CACCCGCACA AGCCCAGGCT 
GCAAGCACCC CGAGGAAGGG CCGAGCCTCG GAGAGCACAG CCAGGAGCTC CTCCTCCGAG 
AGCGAGGATG AGGACGTGAT CCCCGCTACA CAGTGCTTGA CTCCTGGCAT CAGAACCAAT 
GTGGTGACCA TGCCCACTGC CCACCCAAGA ATAGCCCCCA AAGCCAGCAT GGCTGGGGCC 
AGCAGCAGCA AGGAGTCCAG TCGGATATCA GATGGCAAGA AACAGGAGGG ACCAGCCACT 
CAGGTTGACA GTGCTGTGGG AACACTCCCT GCAACAAGTC CCCAGAGCAG CTCCGTCCAG 
GCCAAAGGGA CCAACAAGCT CAGAAAACCT AAGCTTCCTG AGGTCCAGCA GGCCACCAAA 
GCCCCTGAGA GCTCAGATGA CAGTGAGGAC AGCAGCGACA GTTCTTCAGG GAGTGAGGAA 
GATGCTGAAG GGCCCCAGGG GGCCAAGTCG GCCCACACGC TGGGTCCCAC CCCCTCCAGG 
ACAGAGACCC TGGTGGAGGA GACCGCAGCA GAGTCCAGCG AGGATGATGT GGTGGCGCCA 
TCCCAGTCTC TCCTCTCAGG TTATATGACC CCTGGACTAA CCCCAGCCAA TTCCCAGGCC 
TCAAAAGCCA CTCCCAAGCT AGACTCCAGC CCCTCAGTTT CCTCTACTCT GGCCGCCAAA 
GATGACCCAG ATGGCAAGCA GGAGGCAAAG CCCCAACAGG CAGCAGGCAT GTTGTCCCCT 
AAAACAGGTG GAAAAGAGGC TGCTTCAGGC ACCACACCTC AGAAGTCCCG GAAGCCCAAG 
AAAGGGGCTG GGAACCCCCA AGCCTCAACC CTGGCGCTGC AAAGCAACAT CGCCCAGTGC 
CTCCTGGGCC AACCCTGGCC CCTGAATGAG GCCCAGGTGC AGGCCTCAGT GGTGAAGGTC 
CTGACTGAGC TGCTGGAACA GGAAAGAAAG AAGGTGGTGG ACACCACCAA GGAGAGCAGC 
AGGAAGGGCT GGGAGAGCCG CAAGCGCAAG CTATCGGGAG ACCAGCTGGC TGCCAGGACC 
CCCAGGAGCA AGAAGAAGAA GAAGCTGGGG GCCAGGGAAG GTGGGGAGGC CTCTGTTTCC 
CCAGAAAAGA CCTCCACGAC TTCCAAGGGG AAAGCAAAGA GAGACAAAGC GAGTGGTGAT 
GTCAAGGAGA AGAAAGGGAA GGGGTCTCTT GGCTCCCAAG GGGCCAAGGA CGAGCCAGAA 
GAGGAGCTTC AGAAGGGGAT GGGGAAGGTT GAAGGTGGAG ATCAAAGCAA CCCAAAGAGC 
AAGAAGGAGA AGAAGAAATC CGACAAGAGA AAAAAAGACA AAGAAAAAAA AGAAAAGAAG 
AAGAAAGCAA AAAAGGCCTC AACCAAAGAT TCTGAGTCAC CGTCCCAGAA GAAAAAGAAG 
AAAAAGAAGA AGACAGCAGA GCAGACTGTA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016809535.1
CDS	104..4456
Translation	MAEARKRRELLPLIYHHLLRAGYVRAAREVKEQSGQKCFLAQPVTLLDIYTHWQQTSELGRKRKAEEDAALQAKKTRVSDPISTSESSEEEEEAEAETAKATPRLASTNSSVLGADLPSSMKEKAKAETEKAGKTGNSMPHPATGKTVANLLSGKSPRKSAEPSANTTLVSETEEEGSVLAFGAAAKPGMVSAGQADSSSEDTSSSSDETDVEGKPSVKPAQVKASSVSTKESPARKAAPAPGKVGDVTPQVKGGALPPAKKAKKPEEESESSEEGSESEEEAPAGTRSQVKASEKILQVRAASAPAKGTPGKGATPAPHGKAGAVASQTKAGKPEEDSESSSEESSDSEEETPAAKALLQAKASGKTSQVGAASAPAKESPRKGAAPAPPGKTGPAVAKAQAGKREEDSQSSSEESDSEEEAPAQAKPSGKAPQVRAASAPAKESPRKGTAPAPPRKTGPAAAQVQVGKQEEDSGSSSEESDSDREALAAMNAAQVKPLGKSPQVKPASTMGMGPLGKGAGPVPPGKVGPATPSAQVGKWEEDSESSSEESSDSSDGEVPTAVAPAQEKSLGNILQAKPASSPAKGPPQKAGPVAVQVKAEKPMDNSESSEESSDSADSEEAPAVMTAAQAKPALKIPQTKACPKKTNTTASAKVAPVRVGTQAPRKAGTATSPAGSSPAVAGGTQRPAEDSSSSEESDSEEEKTGLAVTVGQAKSVGKGLQVKAASVPLKGSLGQGTAPVLPGKTGPTVTQVKAEKQEDSESSEEESDSEEAAASPAQVKTSVKKTQAKANPAAARAPSAKGTISAPGKVVTAAAQVKQRSPSKVKPPVRNPQNSTVLARGPASVPSVGKAVATAARAQTGPEEDSGSSEEESDSEEEVETLAQAKPSGKTPQIRAALAPAKESPRKGAAPTPPGKTGPSAAQAGKQDDSGSSSEESDSDGEAPAAVTSAQVIKPPLIFVDPNRSPAGPAATPAQAQAASTPRKGRASESTARSSSSESEDEDVIPATQCLTPGIRTNVVTMPTAHPRIAPKASMAGASSSKESSRISDGKKQEGPATQVDSAVGTLPATSPQSSSVQAKGTNKLRKPKLPEVQQATKAPESSDDSEDSSDSSSGSEEDAEGPQGAKSAHTLGPTPSRTETLVEETAAESSEDDVVAPSQSLLSGYMTPGLTPANSQASKATPKLDSSPSVSSTLAAKDDPDGKQEAKPQQAAGMLSPKTGGKEAASGTTPQKSRKPKKGAGNPQASTLALQSNIAQCLLGQPWPLNEAQVQASVVKVLTELLEQERKKVVDTTKESSRKGWESRKRKLSGDQLAARTPRSKKKKKLGAREGGEASVSPEKTSTTSKGKAKRDKASGDVKEKKGKGSLGSQGAKDEPEEELQKGMGKVEGGDQSNPKSKKEKKKSDKRKKDKEKKEKKKKAKKASTKDSESPSQKKKKKKKKTAEQTV

Target ORF information:

RefSeq Version	XM_016954046.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016954046.1

ORF Insert Sequence:

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCAGAGAG CTCGGAAGAG GAGGAGGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCGAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCTTG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGT 
GAGGACACCT CCAGCTCCAG CGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCGCCAGCC 
AAGAAGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CGGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCAT 
GGGAAGGCAG GAGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGTG AGGAGTCGTC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GTGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAAGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG ACTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAGGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCC ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCTGG GAAGGTGGGG CCTGCAACCC CCTCGGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCT 
GCCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CGTCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGTCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCGGC CTCAGTGCCT CTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAGTCAGAC AGTGAGGAGG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGTCAAA 
CAGAGATCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCGGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GTGGAGACGC TGGCTCAGGC GAAGCCTTCA GGGAAGACCC CCCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AAGCACCGGC AGCTGTGACC TCTGCCCAGG TGATTAAACC CCCTCTGATT 
TTTGTCGACC CTAATCGTAG TCCAGCTGGC CCAGCTGCTA CACCCGCACA AGCCCAGGCT 
GCAAGCACCC CGAGGAAGGG CCGAGCCTCG GAGAGCACAG CCAGGAGCTC CTCCTCCGAG 
AGCGAGGATG AGGACGTGAT CCCCGCTACA CAGTGCTTGA CTCCTGGCAT CAGAACCAAT 
GTGGTGACCA TGCCCACTGC CCACCCAAGA ATAGCCCCCA AAGCCAGCAT GGCTGGGGCC 
AGCAGCAGCA AGGAGTCCAG TCGGATATCA GATGGCAAGA AACAGGAGGG ACCAGCCACT 
CAGGTTGACA GTGCTGTGGG AACACTCCCT GCAACAAGTC CCCAGAGCAG CTCCGTCCAG 
GCCAAAGGGA CCAACAAGCT CAGAAAACCT AAGCTTCCTG AGGTCCAGCA GGCCACCAAA 
GCCCCTGAGA GCTCAGATGA CAGTGAGGAC AGCAGCGACA GTTCTTCAGG GAGTGAGGAA 
GATGCTGAAG GGCCCCAGGG GGCCAAGTCG GCCCACACGC TGGGTCCCAC CCCCTCCAGG 
ACAGAGACCC TGGTGGAGGA GACCGCAGCA GAGTCCAGCG AGGATGATGT GGTGGCGCCA 
TCCCAGTCTC TCCTCTCAGG TTATATGACC CCTGGACTAA CCCCAGCCAA TTCCCAGGCC 
TCAAAAGCCA CTCCCAAGCT AGACTCCAGC CCCTCAGTTT CCTCTACTCT GGCCGCCAAA 
GATGACCCAG ATGGCAAGCA GGAGGCAAAG CCCCAACAGG CAGCAGGCAT GTTGTCCCCT 
AAAACAGGTG GAAAAGAGGC TGCTTCAGGC ACCACACCTC AGAAGTCCCG GAAGCCCAAG 
AAAGGGGCTG GGAACCCCCA AGCCTCAACC CTGGCGCTGC AAAGCAACAT CGCCCAGTGC 
CTCCTGGGCC AACCCTGGCC CCTGAATGAG GCCCAGGTGC AGGCCTCAGT GGTGAAGGTC 
CTGACTGAGC TGCTGGAACA GGAAAGAAAG AAGGTGGTGG ACACCACCAA GGAGAGCAGC 
AGGAAGGGCT GGGAGAGCCG CAAGCGCAAG CTATCGGGAG ACCAGCTGGC TGCCAGGACC 
CCCAGGAGCA AGAAGAAGAA GAAGCTGGGG GCCAGGGAAG GTGGGGAGGC CTCTGTTTCC 
CCAGAAAAGA CCTCCACGAC TTCCAAGGGG AAAGCAAAGA GAGACAAAGC GAGTGGTGAT 
GTCAAGGAGA AGAAAGGGAA GGGGTCTCTT GGCTCCCAAG GGGCCAAGGA CGAGCCAGAA 
GAGGAGCTTC AGAAGGGGAT GGGGAAGGTT GAAGGTGGAG ATCAAAGCAA CCCAAAGAGC 
AAGAAGGAGA AGAAGAAATC CGACAAGAGA AAAAAAGACA AAGAAAAAAA AGAAAAGAAG 
AAGAAAGCAA AAAAGGCCTC AACCAAAGAT TCTGAGTCAC CGTCCCAGAA GAAAAAGAAG 
AAAAAGAAGA AGACAGCAGA GCAGACTGTA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_016954047.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016809536.1 Pan troglodytes treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X7, mRNA.

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CloneID	OPc85513
Clone ID Related Accession (Same CDS sequence)	XM_016954047.1
Accession Version	XM_016954047.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4236bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	treacle protein isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006472.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCAGAGAG CTCGGAAGAG GAGGAGGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCGAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCTTG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGT 
GAGGACACCT CCAGCTCCAG CGATGAGACA GACGTGGAGG TAAAGGCCTC TGAAAAAATT 
CTCCAGGTCA GAGCTGCCTC GGCCCCTGCC AAGGGGACCC CTGGGAAAGG GGCTACCCCA 
GCACCCCATG GGAAGGCAGG AGCTGTAGCC TCCCAGACCA AGGCAGGGAA GCCAGAGGAG 
GACTCAGAGA GCAGCAGTGA GGAGTCGTCT GACAGTGAGG AGGAGACGCC AGCTGCCAAG 
GCCCTGCTTC AGGCGAAGGC CTCAGGAAAA ACCTCTCAGG TCGGAGCTGC CTCAGCCCCT 
GCCAAGGAGT CCCCCAGGAA AGGAGCTGCC CCAGCGCCCC CTGGGAAGAC AGGGCCTGCA 
GTTGCCAAGG CCCAGGCGGG GAAGCGGGAG GAGGACTCGC AGAGCAGCAG TGAGGAATCG 
GACAGTGAGG AGGAGGCGCC TGCTCAGGCG AAGCCTTCAG GGAAGGCCCC CCAAGTCAGA 
GCCGCCTCGG CCCCTGCCAA GGAGTCCCCC AGGAAAGGGA CTGCCCCAGC ACCTCCTAGG 
AAAACAGGGC CTGCAGCCGC CCAGGTCCAG GTGGGGAAGC AGGAGGAGGA CTCAGGAAGC 
AGCAGCGAGG AGTCAGACAG TGACAGAGAG GCACTGGCAG CCATGAATGC AGCTCAGGTG 
AAGCCCTTGG GGAAAAGCCC CCAGGTGAAA CCTGCCTCCA CCATGGGCAT GGGGCCCTTG 
GGGAAAGGCG CCGGCCCAGT GCCACCTGGG AAGGTGGGGC CTGCAACCCC CTCGGCCCAG 
GTGGGGAAGT GGGAGGAGGA CTCAGAGAGC AGTAGTGAGG AGTCATCAGA CAGCAGTGAT 
GGAGAGGTGC CCACAGCTGT GGCCCCGGCT CAGGAAAAGT CCTTGGGGAA CATCCTCCAG 
GCCAAACCTG CCTCCAGTCC TGCCAAGGGG CCCCCTCAGA AGGCAGGGCC TGTAGCCGTC 
CAGGTCAAGG CTGAAAAGCC CATGGACAAC TCGGAGAGCA GCGAGGAGTC GTCGGACAGT 
GCGGACAGTG AGGAGGCACC AGCAGTCATG ACTGCAGCTC AGGCAAAACC AGCTCTGAAA 
ATTCCTCAGA CCAAGGCCTG CCCAAAGAAA ACCAATACCA CTGCATCTGC CAAGGTCGCC 
CCTGTGCGAG TGGGCACCCA AGCCCCCCGG AAAGCAGGAA CTGCGACTTC TCCAGCAGGC 
TCATCCCCAG CTGTGGCTGG GGGCACCCAG AGACCAGCAG AGGATTCTTC AAGCAGTGAG 
GAATCAGATA GTGAGGAAGA GAAGACAGGT CTTGCAGTAA CCGTGGGACA GGCAAAGTCT 
GTGGGGAAAG GCCTCCAGGT GAAAGCGGCC TCAGTGCCTC TCAAGGGGTC CTTGGGGCAA 
GGGACTGCTC CAGTACTCCC TGGGAAGACG GGGCCTACAG TCACCCAGGT GAAAGCTGAA 
AAGCAGGAAG ACTCTGAGAG CAGTGAGGAG GAGTCAGACA GTGAGGAGGC AGCTGCATCT 
CCAGCACAGG TGAAAACCTC AGTAAAGAAA ACCCAGGCCA AAGCCAACCC AGCTGCCGCC 
AGAGCACCTT CAGCAAAAGG GACAATTTCA GCCCCTGGAA AAGTTGTCAC TGCAGCTGCT 
CAAGTCAAAC AGAGATCTCC ATCCAAGGTG AAGCCACCAG TGAGAAACCC CCAGAACAGT 
ACCGTCTTGG CGAGGGGCCC AGCATCTGTG CCATCTGTGG GGAAGGCCGT GGCTACAGCA 
GCTCGGGCCC AGACAGGGCC AGAGGAGGAC TCAGGGAGCA GTGAGGAGGA GTCAGACAGT 
GAGGAGGAGG TGGAGACGCT GGCTCAGGCG AAGCCTTCAG GGAAGACCCC CCAGATCAGA 
GCTGCCTTGG CTCCTGCCAA GGAGTCCCCC AGGAAAGGGG CTGCCCCAAC ACCTCCTGGG 
AAGACAGGGC CTTCGGCTGC CCAGGCAGGG AAGCAGGATG ACTCAGGGAG CAGCAGCGAG 
GAATCAGACA GTGATGGGGA AGCACCGGCA GCTGTGACCT CTGCCCAGGT GATTAAACCC 
CCTCTGATTT TTGTCGACCC TAATCGTAGT CCAGCTGGCC CAGCTGCTAC ACCCGCACAA 
GCCCAGGCTG CAAGCACCCC GAGGAAGGGC CGAGCCTCGG AGAGCACAGC CAGGAGCTCC 
TCCTCCGAGA GCGAGGATGA GGACGTGATC CCCGCTACAC AGTGCTTGAC TCCTGGCATC 
AGAACCAATG TGGTGACCAT GCCCACTGCC CACCCAAGAA TAGCCCCCAA AGCCAGCATG 
GCTGGGGCCA GCAGCAGCAA GGAGTCCAGT CGGATATCAG ATGGCAAGAA ACAGGAGGGA 
CCAGCCACTC AGGTGTCAAA GAAGAACCCA GCTTCCCTCC CACTGACCCA GGCTACCCTG 
AAGGTCCTCG CCCAGAAAGC CAGTGAGGCT CAGCCTCCTG TTGCCAGGAC CCAGCCTTCA 
AGTGGGGTTG ACAGTGCTGT GGGAACACTC CCTGCAACAA GTCCCCAGAG CAGCTCCGTC 
CAGGCCAAAG GGACCAACAA GCTCAGAAAA CCTAAGCTTC CTGAGGTCCA GCAGGCCACC 
AAAGCCCCTG AGAGCTCAGA TGACAGTGAG GACAGCAGCG ACAGTTCTTC AGGGAGTGAG 
GAAGATGCTG AAGGGCCCCA GGGGGCCAAG TCGGCCCACA CGCTGGGTCC CACCCCCTCC 
AGGACAGAGA CCCTGGTGGA GGAGACCGCA GCAGAGTCCA GCGAGGATGA TGTGGTGGCG 
CCATCCCAGT CTCTCCTCTC AGGTTATATG ACCCCTGGAC TAACCCCAGC CAATTCCCAG 
GCCTCAAAAG CCACTCCCAA GCTAGACTCC AGCCCCTCAG TTTCCTCTAC TCTGGCCGCC 
AAAGATGACC CAGATGGCAA GCAGGAGGCA AAGCCCCAAC AGGCAGCAGG CATGTTGTCC 
CCTAAAACAG GTGGAAAAGA GGCTGCTTCA GGCACCACAC CTCAGAAGTC CCGGAAGCCC 
AAGAAAGGGG CTGGGAACCC CCAAGCCTCA ACCCTGGCGC TGCAAAGCAA CATCGCCCAG 
TGCCTCCTGG GCCAACCCTG GCCCCTGAAT GAGGCCCAGG TGCAGGCCTC AGTGGTGAAG 
GTCCTGACTG AGCTGCTGGA ACAGGAAAGA AAGAAGGTGG TGGACACCAC CAAGGAGAGC 
AGCAGGAAGG GCTGGGAGAG CCGCAAGCGC AAGCTATCGG GAGACCAGCT GGCTGCCAGG 
ACCCCCAGGA GCAAGAAGAA GAAGAAGCTG GGGGCCAGGG AAGGTGGGGA GGCCTCTGTT 
TCCCCAGAAA AGACCTCCAC GACTTCCAAG GGGAAAGCAA AGAGAGACAA AGCGAGTGGT 
GATGTCAAGG AGAAGAAAGG GAAGGGGTCT CTTGGCTCCC AAGGGGCCAA GGACGAGCCA 
GAAGAGGAGC TTCAGAAGGG GATGGGGAAG GTTGAAGGTG GAGATCAAAG CAACCCAAAG 
AGCAAGAAGG AGAAGAAGAA ATCCGACAAG AGAAAAAAAG ACAAAGAAAA AAAAGAAAAG 
AAGAAGAAAG CAAAAAAGGC CTCAACCAAA GATTCTGAGT CACCGTCCCA GAAGAAAAAG 
AAGAAAAAGA AGAAGACAGC AGAGCAGACT GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016809536.1
CDS	104..4339
Translation	MAEARKRRELLPLIYHHLLRAGYVRAAREVKEQSGQKCFLAQPVTLLDIYTHWQQTSELGRKRKAEEDAALQAKKTRVSDPISTSESSEEEEEAEAETAKATPRLASTNSSVLGADLPSSMKEKAKAETEKAGKTGNSMPHPATGKTVANLLSGKSPRKSAEPSANTTLVSETEEEGSVLAFGAAAKPGMVSAGQADSSSEDTSSSSDETDVEVKASEKILQVRAASAPAKGTPGKGATPAPHGKAGAVASQTKAGKPEEDSESSSEESSDSEEETPAAKALLQAKASGKTSQVGAASAPAKESPRKGAAPAPPGKTGPAVAKAQAGKREEDSQSSSEESDSEEEAPAQAKPSGKAPQVRAASAPAKESPRKGTAPAPPRKTGPAAAQVQVGKQEEDSGSSSEESDSDREALAAMNAAQVKPLGKSPQVKPASTMGMGPLGKGAGPVPPGKVGPATPSAQVGKWEEDSESSSEESSDSSDGEVPTAVAPAQEKSLGNILQAKPASSPAKGPPQKAGPVAVQVKAEKPMDNSESSEESSDSADSEEAPAVMTAAQAKPALKIPQTKACPKKTNTTASAKVAPVRVGTQAPRKAGTATSPAGSSPAVAGGTQRPAEDSSSSEESDSEEEKTGLAVTVGQAKSVGKGLQVKAASVPLKGSLGQGTAPVLPGKTGPTVTQVKAEKQEDSESSEEESDSEEAAASPAQVKTSVKKTQAKANPAAARAPSAKGTISAPGKVVTAAAQVKQRSPSKVKPPVRNPQNSTVLARGPASVPSVGKAVATAARAQTGPEEDSGSSEEESDSEEEVETLAQAKPSGKTPQIRAALAPAKESPRKGAAPTPPGKTGPSAAQAGKQDDSGSSSEESDSDGEAPAAVTSAQVIKPPLIFVDPNRSPAGPAATPAQAQAASTPRKGRASESTARSSSSESEDEDVIPATQCLTPGIRTNVVTMPTAHPRIAPKASMAGASSSKESSRISDGKKQEGPATQVSKKNPASLPLTQATLKVLAQKASEAQPPVARTQPSSGVDSAVGTLPATSPQSSSVQAKGTNKLRKPKLPEVQQATKAPESSDDSEDSSDSSSGSEEDAEGPQGAKSAHTLGPTPSRTETLVEETAAESSEDDVVAPSQSLLSGYMTPGLTPANSQASKATPKLDSSPSVSSTLAAKDDPDGKQEAKPQQAAGMLSPKTGGKEAASGTTPQKSRKPKKGAGNPQASTLALQSNIAQCLLGQPWPLNEAQVQASVVKVLTELLEQERKKVVDTTKESSRKGWESRKRKLSGDQLAARTPRSKKKKKLGAREGGEASVSPEKTSTTSKGKAKRDKASGDVKEKKGKGSLGSQGAKDEPEEELQKGMGKVEGGDQSNPKSKKEKKKSDKRKKDKEKKEKKKKAKKASTKDSESPSQKKKKKKKKTAEQTV

Target ORF information:

RefSeq Version	XM_016954047.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016954047.1

ORF Insert Sequence:

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCAGAGAG CTCGGAAGAG GAGGAGGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCGAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCTTG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGT 
GAGGACACCT CCAGCTCCAG CGATGAGACA GACGTGGAGG TAAAGGCCTC TGAAAAAATT 
CTCCAGGTCA GAGCTGCCTC GGCCCCTGCC AAGGGGACCC CTGGGAAAGG GGCTACCCCA 
GCACCCCATG GGAAGGCAGG AGCTGTAGCC TCCCAGACCA AGGCAGGGAA GCCAGAGGAG 
GACTCAGAGA GCAGCAGTGA GGAGTCGTCT GACAGTGAGG AGGAGACGCC AGCTGCCAAG 
GCCCTGCTTC AGGCGAAGGC CTCAGGAAAA ACCTCTCAGG TCGGAGCTGC CTCAGCCCCT 
GCCAAGGAGT CCCCCAGGAA AGGAGCTGCC CCAGCGCCCC CTGGGAAGAC AGGGCCTGCA 
GTTGCCAAGG CCCAGGCGGG GAAGCGGGAG GAGGACTCGC AGAGCAGCAG TGAGGAATCG 
GACAGTGAGG AGGAGGCGCC TGCTCAGGCG AAGCCTTCAG GGAAGGCCCC CCAAGTCAGA 
GCCGCCTCGG CCCCTGCCAA GGAGTCCCCC AGGAAAGGGA CTGCCCCAGC ACCTCCTAGG 
AAAACAGGGC CTGCAGCCGC CCAGGTCCAG GTGGGGAAGC AGGAGGAGGA CTCAGGAAGC 
AGCAGCGAGG AGTCAGACAG TGACAGAGAG GCACTGGCAG CCATGAATGC AGCTCAGGTG 
AAGCCCTTGG GGAAAAGCCC CCAGGTGAAA CCTGCCTCCA CCATGGGCAT GGGGCCCTTG 
GGGAAAGGCG CCGGCCCAGT GCCACCTGGG AAGGTGGGGC CTGCAACCCC CTCGGCCCAG 
GTGGGGAAGT GGGAGGAGGA CTCAGAGAGC AGTAGTGAGG AGTCATCAGA CAGCAGTGAT 
GGAGAGGTGC CCACAGCTGT GGCCCCGGCT CAGGAAAAGT CCTTGGGGAA CATCCTCCAG 
GCCAAACCTG CCTCCAGTCC TGCCAAGGGG CCCCCTCAGA AGGCAGGGCC TGTAGCCGTC 
CAGGTCAAGG CTGAAAAGCC CATGGACAAC TCGGAGAGCA GCGAGGAGTC GTCGGACAGT 
GCGGACAGTG AGGAGGCACC AGCAGTCATG ACTGCAGCTC AGGCAAAACC AGCTCTGAAA 
ATTCCTCAGA CCAAGGCCTG CCCAAAGAAA ACCAATACCA CTGCATCTGC CAAGGTCGCC 
CCTGTGCGAG TGGGCACCCA AGCCCCCCGG AAAGCAGGAA CTGCGACTTC TCCAGCAGGC 
TCATCCCCAG CTGTGGCTGG GGGCACCCAG AGACCAGCAG AGGATTCTTC AAGCAGTGAG 
GAATCAGATA GTGAGGAAGA GAAGACAGGT CTTGCAGTAA CCGTGGGACA GGCAAAGTCT 
GTGGGGAAAG GCCTCCAGGT GAAAGCGGCC TCAGTGCCTC TCAAGGGGTC CTTGGGGCAA 
GGGACTGCTC CAGTACTCCC TGGGAAGACG GGGCCTACAG TCACCCAGGT GAAAGCTGAA 
AAGCAGGAAG ACTCTGAGAG CAGTGAGGAG GAGTCAGACA GTGAGGAGGC AGCTGCATCT 
CCAGCACAGG TGAAAACCTC AGTAAAGAAA ACCCAGGCCA AAGCCAACCC AGCTGCCGCC 
AGAGCACCTT CAGCAAAAGG GACAATTTCA GCCCCTGGAA AAGTTGTCAC TGCAGCTGCT 
CAAGTCAAAC AGAGATCTCC ATCCAAGGTG AAGCCACCAG TGAGAAACCC CCAGAACAGT 
ACCGTCTTGG CGAGGGGCCC AGCATCTGTG CCATCTGTGG GGAAGGCCGT GGCTACAGCA 
GCTCGGGCCC AGACAGGGCC AGAGGAGGAC TCAGGGAGCA GTGAGGAGGA GTCAGACAGT 
GAGGAGGAGG TGGAGACGCT GGCTCAGGCG AAGCCTTCAG GGAAGACCCC CCAGATCAGA 
GCTGCCTTGG CTCCTGCCAA GGAGTCCCCC AGGAAAGGGG CTGCCCCAAC ACCTCCTGGG 
AAGACAGGGC CTTCGGCTGC CCAGGCAGGG AAGCAGGATG ACTCAGGGAG CAGCAGCGAG 
GAATCAGACA GTGATGGGGA AGCACCGGCA GCTGTGACCT CTGCCCAGGT GATTAAACCC 
CCTCTGATTT TTGTCGACCC TAATCGTAGT CCAGCTGGCC CAGCTGCTAC ACCCGCACAA 
GCCCAGGCTG CAAGCACCCC GAGGAAGGGC CGAGCCTCGG AGAGCACAGC CAGGAGCTCC 
TCCTCCGAGA GCGAGGATGA GGACGTGATC CCCGCTACAC AGTGCTTGAC TCCTGGCATC 
AGAACCAATG TGGTGACCAT GCCCACTGCC CACCCAAGAA TAGCCCCCAA AGCCAGCATG 
GCTGGGGCCA GCAGCAGCAA GGAGTCCAGT CGGATATCAG ATGGCAAGAA ACAGGAGGGA 
CCAGCCACTC AGGTGTCAAA GAAGAACCCA GCTTCCCTCC CACTGACCCA GGCTACCCTG 
AAGGTCCTCG CCCAGAAAGC CAGTGAGGCT CAGCCTCCTG TTGCCAGGAC CCAGCCTTCA 
AGTGGGGTTG ACAGTGCTGT GGGAACACTC CCTGCAACAA GTCCCCAGAG CAGCTCCGTC 
CAGGCCAAAG GGACCAACAA GCTCAGAAAA CCTAAGCTTC CTGAGGTCCA GCAGGCCACC 
AAAGCCCCTG AGAGCTCAGA TGACAGTGAG GACAGCAGCG ACAGTTCTTC AGGGAGTGAG 
GAAGATGCTG AAGGGCCCCA GGGGGCCAAG TCGGCCCACA CGCTGGGTCC CACCCCCTCC 
AGGACAGAGA CCCTGGTGGA GGAGACCGCA GCAGAGTCCA GCGAGGATGA TGTGGTGGCG 
CCATCCCAGT CTCTCCTCTC AGGTTATATG ACCCCTGGAC TAACCCCAGC CAATTCCCAG 
GCCTCAAAAG CCACTCCCAA GCTAGACTCC AGCCCCTCAG TTTCCTCTAC TCTGGCCGCC 
AAAGATGACC CAGATGGCAA GCAGGAGGCA AAGCCCCAAC AGGCAGCAGG CATGTTGTCC 
CCTAAAACAG GTGGAAAAGA GGCTGCTTCA GGCACCACAC CTCAGAAGTC CCGGAAGCCC 
AAGAAAGGGG CTGGGAACCC CCAAGCCTCA ACCCTGGCGC TGCAAAGCAA CATCGCCCAG 
TGCCTCCTGG GCCAACCCTG GCCCCTGAAT GAGGCCCAGG TGCAGGCCTC AGTGGTGAAG 
GTCCTGACTG AGCTGCTGGA ACAGGAAAGA AAGAAGGTGG TGGACACCAC CAAGGAGAGC 
AGCAGGAAGG GCTGGGAGAG CCGCAAGCGC AAGCTATCGG GAGACCAGCT GGCTGCCAGG 
ACCCCCAGGA GCAAGAAGAA GAAGAAGCTG GGGGCCAGGG AAGGTGGGGA GGCCTCTGTT 
TCCCCAGAAA AGACCTCCAC GACTTCCAAG GGGAAAGCAA AGAGAGACAA AGCGAGTGGT 
GATGTCAAGG AGAAGAAAGG GAAGGGGTCT CTTGGCTCCC AAGGGGCCAA GGACGAGCCA 
GAAGAGGAGC TTCAGAAGGG GATGGGGAAG GTTGAAGGTG GAGATCAAAG CAACCCAAAG 
AGCAAGAAGG AGAAGAAGAA ATCCGACAAG AGAAAAAAAG ACAAAGAAAA AAAAGAAAAG 
AAGAAGAAAG CAAAAAAGGC CTCAACCAAA GATTCTGAGT CACCGTCCCA GAAGAAAAAG 
AAGAAAAAGA AGAAGACAGC AGAGCAGACT GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_016954048.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016809537.1 Pan troglodytes treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X8, mRNA.

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CloneID	OPc85514
Clone ID Related Accession (Same CDS sequence)	XM_016954048.1
Accession Version	XM_016954048.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2877bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	treacle protein isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006472.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCAGAGAG CTCGGAAGAG GAGGAGGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCGAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCTTG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGT 
GAGGACACCT CCAGCTCCAG CGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCGCCAGCC 
AAGAAGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CGGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCAT 
GGGAAGGCAG GAGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGTG AGGAGTCGTC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GTGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAAGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG ACTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAGGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCC ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCTGG GAAGGTGGGG CCTGCAACCC CCTCGGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCT 
GCCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CGTCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGTCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCGGC CTCAGTGCCT CTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAGTCAGAC AGTGAGGAGG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGTCAAA 
CAGAGATCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCGGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GTGGAGACGC TGGCTCAGGC GAAGCCTTCA GGGAAGACCC CCCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AAGCACCGGC AGCTGTGACC TCTGCCCAGG ACCAGGAGTC TTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016809537.1
CDS	104..2980
Translation	MAEARKRRELLPLIYHHLLRAGYVRAAREVKEQSGQKCFLAQPVTLLDIYTHWQQTSELGRKRKAEEDAALQAKKTRVSDPISTSESSEEEEEAEAETAKATPRLASTNSSVLGADLPSSMKEKAKAETEKAGKTGNSMPHPATGKTVANLLSGKSPRKSAEPSANTTLVSETEEEGSVLAFGAAAKPGMVSAGQADSSSEDTSSSSDETDVEGKPSVKPAQVKASSVSTKESPARKAAPAPGKVGDVTPQVKGGALPPAKKAKKPEEESESSEEGSESEEEAPAGTRSQVKASEKILQVRAASAPAKGTPGKGATPAPHGKAGAVASQTKAGKPEEDSESSSEESSDSEEETPAAKALLQAKASGKTSQVGAASAPAKESPRKGAAPAPPGKTGPAVAKAQAGKREEDSQSSSEESDSEEEAPAQAKPSGKAPQVRAASAPAKESPRKGTAPAPPRKTGPAAAQVQVGKQEEDSGSSSEESDSDREALAAMNAAQVKPLGKSPQVKPASTMGMGPLGKGAGPVPPGKVGPATPSAQVGKWEEDSESSSEESSDSSDGEVPTAVAPAQEKSLGNILQAKPASSPAKGPPQKAGPVAVQVKAEKPMDNSESSEESSDSADSEEAPAVMTAAQAKPALKIPQTKACPKKTNTTASAKVAPVRVGTQAPRKAGTATSPAGSSPAVAGGTQRPAEDSSSSEESDSEEEKTGLAVTVGQAKSVGKGLQVKAASVPLKGSLGQGTAPVLPGKTGPTVTQVKAEKQEDSESSEEESDSEEAAASPAQVKTSVKKTQAKANPAAARAPSAKGTISAPGKVVTAAAQVKQRSPSKVKPPVRNPQNSTVLARGPASVPSVGKAVATAARAQTGPEEDSGSSEEESDSEEEVETLAQAKPSGKTPQIRAALAPAKESPRKGAAPTPPGKTGPSAAQAGKQDDSGSSSEESDSDGEAPAAVTSAQDQESS

Target ORF information:

RefSeq Version	XM_016954048.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016954048.1

ORF Insert Sequence:

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCAGAGAG CTCGGAAGAG GAGGAGGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCGAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCTTG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGT 
GAGGACACCT CCAGCTCCAG CGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCGCCAGCC 
AAGAAGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CGGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCAT 
GGGAAGGCAG GAGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGTG AGGAGTCGTC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GTGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAAGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG ACTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAGGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCC ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCTGG GAAGGTGGGG CCTGCAACCC CCTCGGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCT 
GCCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CGTCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGTCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCGGC CTCAGTGCCT CTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAGTCAGAC AGTGAGGAGG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGTCAAA 
CAGAGATCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCGGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GTGGAGACGC TGGCTCAGGC GAAGCCTTCA GGGAAGACCC CCCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AAGCACCGGC AGCTGTGACC TCTGCCCAGG ACCAGGAGTC TTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	TCOF1
Entrez Gene ID	471696
Full Name	treacle ribosome biogenesis factor 1
General protein information	Preferred Names treacle ribosome biogenesis factor 1 Names treacle protein Treacher Collins-Franceschetti syndrome 1
Gene Type	protein-coding
Organism	Pan troglodytes(chimpanzee)

Homo sapiens (human)	TCOF1	NP_001128717.1
Bos taurus (cattle)	TCOF1	XP_002689339.2
Rattus norvegicus (Norway rat)	Tcof1	NP_001099613.1
Canis lupus familiaris (dog)	TCOF1	NP_001003057.1
Pan troglodytes (chimpanzee)	TCOF1	XP_527074.4
Mus musculus (house mouse)	Tcof1	NP_001185913.1
	TCOF1	NP_001035041.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

TCOF1 cDNA ORF clone, Pan troglodytes(chimpanzee)

Gene

Clones

gRNAs

Target ORF information:

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