SEPHS2 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	SEPHS2
Entrez Gene ID	512060
Full Name	selenophosphate synthetase 2
Synonyms	SPS2
Gene Type	protein-coding
Organism	Bos taurus(cattle)
Summary	This gene encodes an enzyme that catalyzes the production of monoselenophosphate (MSP) from selenide and ATP. MSP is the selenium donor required for synthesis of selenocysteine (Sec), which is co-translationally incorporated into selenoproteins at in-frame UGA codons that normally signal translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. This protein is itself a selenoprotein containing a Sec residue at its active site, suggesting the existence of an autoregulatory mechanism. It is preferentially expressed in tissues implicated in the synthesis of selenoproteins and in sites of blood cell development. A pseudogene of this locus has been identified on chromosome 4. [provided by RefSeq, Aug 2017].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001114732.2	NP_001108204.2	selenide, water dikinase 2
NM_001114732.1	NP_001108204.1	selenide, water dikinase 2
NM_001114732.3	NP_001108204.2	selenide, water dikinase 2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Sephs2	NP_033292.2
Bos taurus (cattle)	SEPHS2	NP_001108204.1
Rattus norvegicus (Norway rat)	Sephs2	NP_001073358.2
Xenopus tropicalis (tropical clawed frog)	sephs2	NP_001072895.2
Homo sapiens (human)	SEPHS2	NP_036380.2
Pan troglodytes (chimpanzee)	SEPHS2	NP_001108203.1
	SEPHS2	NP_001152892.1
Canis lupus familiaris (dog)	SEPHS2	NP_001108208.1
Danio rerio (zebrafish)	sps2	NP_001004295.2

Gene Ontology
Bibliography

Related articles in PubMed

Selenoprotein Gene Nomenclature.
Gladyshev VN, Arn?r ES, Berry MJ, Brigelius-Floh? R, Bruford EA, Burk RF, Carlson BA, Castellano S, Chavatte L, Conrad M, Copeland PR, Diamond AM, Driscoll DM, Ferreiro A, Floh? L, Green FR, Guig? R, Handy DE, Hatfield DL, Hesketh J, Hoffmann PR, Holmgren A, Hondal RJ, Howard MT, Huang K, Kim HY, Kim IY, K?hrle J, Krol A, Kryukov GV, Lee BJ, Lee BC, Lei XG, Liu Q, Lescure A, Lobanov AV, Loscalzo J, Maiorino M, Mariotti M, Sandeep Prabhu K, Rayman MP, Rozovsky S, Salinas G, Schmidt EE, Schomburg L, Schweizer U, Simonovi? M, Sunde RA, Tsuji PA, Tweedie S, Ursini F, Whanger PD, Zhang Y
The Journal of biological chemistry291(46)24036-24040(2016 Nov)

Composition and evolution of the vertebrate and mammalian selenoproteomes.
Mariotti M, Ridge PG, Zhang Y, Lobanov AV, Pringle TH, Guigo R, Hatfield DL, Gladyshev VN
PloS one7(3)e33066(2012)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Active bovine selenophosphate synthetase 2, not having selenocysteine.
Furumiya K, Kanaya K, Tanabe K, Tanaka Y, Mizutani T
Molecular biology reports35(4)541-9(2008 Dec)

Analysis of bovine mammary gland EST and functional annotation of the Bos taurus gene index.
Sonstegard TS, Capuco AV, White J, Van Tassell CP, Connor EE, Cho J, Sultana R, Shade L, Wray JE, Wells KD, Quackenbush J
Mammalian genome : official journal of the International Mammalian Genome Society13(7)373-9(2002 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Thus, bovine active selenophosphate synthetase 2 of molecular mass 33 kDa does not contain selenocysteine.
Title: Active bovine selenophosphate synthetase 2, not having selenocysteine.

The following SEPHS2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SEPHS2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa40410	NM_001114732.1 Latest version!	Bos taurus selenophosphate synthetase 2 (SEPHS2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OBa216292	NM_001114732.2 Latest version!	Bos taurus selenophosphate synthetase 2 (SEPHS2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OBa239160	NM_001114732.3 Latest version!	Bos taurus selenophosphate synthetase 2 (SEPHS2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SEPHS2 ( NM_001114732.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001108204.1 Bos taurus selenophosphate synthetase 2 (SEPHS2), mRNA.

Price & Availability↑

CloneID	OBa40410
Clone ID Related Accession (Same CDS sequence)	NM_001114732.1
Accession Version	NM_001114732.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1356bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-23
Organism	Bos taurus(Cattle)
Product	selenide, water dikinase 2
Comment	VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DV810180.1, AV604903.1, DV924272.1, DN533754.1, DV799835.1 and CV981553.1. On Aug 18, 2008 this sequence version replaced XM_001790642.1. ##Evidence-Data-START## CDS exon combination :: EV678001.1, DV791142.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN02403656 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## protein contains selenocysteine :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGAAG TCGCGGCGAC TGGTGCCGGC GGAGAGGCTA TGGCGGCAGT GGCGGCGGGG 
GAAGGCTCCT CGGGCCCCGT GGGCTTGCCT CAAGGCCGGG GCTTCTCGAG CTACCGGCCC 
TTCGAGCCCC AGGCGTTGGG TCTCAGCCCG AGCTGGCGGC TCACCGGCTT CTCTGGCATG 
AAGGGCTGAG GCTGCAAGGT CCCCCAGGAG ACGCTGCTCA AACTCCTGGC GGGACTGACG 
CGACCGGAGG TGCTGCCCGC GCCGGGCCGG GGCCTGGTCG GAGGCCTTGA AGAGACGGCC 
CAGGAAGCCG GTCTGCCGGT GAGGGCGGAA CCCAGCCCTC CCTTCCCCAC CCTGGGCATT 
GGGATGGACT CCTGCGTCAT ACCCCTGAGA CACGGGGGCC TGTCCTTGGT GCAGACCACC 
GACTTCTTTT ACCCGCTGGT GGAAGATCCC TACATGATGG GGCGCATCGC ATGTGCCAAC 
GTGCTGAGTG ACCTTTACGC GATGGGCATT ACTGAGTGTG ACAACATGTT GATGCTACTC 
AGCGTCAGCC AGAGTATGCC TGAGGAGGAG CGAGAAAAGA TAACACCACT CATGATCAAA 
GGCTTTCGAG ATGCTGCCGA GGAAGGAGGG ACTGCAGTGA CTGGTGGGCA AACGGTGGTA 
AACCCTTGGA TTATCATCGG TGGAGTTGCC ACTGTAGTAT GTCAACCAAA TGAATTCATC 
CTGCCTGACA GTGCCGTTGT TGGGGATGTG CTCGTGTTAA CTAAACCCTT AGGAACTCAG 
GTTGCCGTCA ATGCCCACCA ATGGCTGGAT AATCCTGAAC GATGGAATAA GATAAAGATG 
GTGGTCTCCA GAGAAGAGGT AGAGCTGGGT TATCAGGAAG CCATGTTCAA CATGGCTACC 
CTCAATAGAA TTGCTGCCGG GCTGATGCAC ACGTTTAATG CCCATGCAGC CACAGATATC 
ACTGGCTTTG GCATTCTGGG TCACTCTCAG AACCTTGCAA AACAACAAAG AAATGAGGTC 
TCCTTTGTCA TTCATAATCT GCCCATCATT GCCAAGATGG CTGCCATCAG CAAGGCCAGC 
GGGCGCTTCG GTCTCCTGCA AGGAACCTCA GCAGAAACTT CTGGGGGGCT ACTAATTTGT 
CTGCCAAGAG AACAGGCAGC TCGCTTTTGT TCAGAAATCA AATCTTCAAA GTATGGAGAG 
GGTCATCAAG CCTGGATCGT CGGTATTGTG GAAAAGGGAA ACCGGACAGC CCGGATCATT 
GACAAGCCTC GCGTTATTGA GGTCCTACCT CGTGGGGCCA CTGCTACTCC TCTTGTTCCT 
GAGAACTCCA GTGCCTCCTC TGAGCCTAGC TTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001108204.1
CDS	162..1517
Translation	MAEVAATGAGGEAMAAVAAGEGSSGPVGLPQGRGFSSYRPFEPQALGLSPSWRLTGFSGMKG*GCKVPQETLLKLLAGLTRPEVLPAPGRGLVGGLEETAQEAGLPVRAEPSPPFPTLGIGMDSCVIPLRHGGLSLVQTTDFFYPLVEDPYMMGRIACANVLSDLYAMGITECDNMLMLLSVSQSMPEEEREKITPLMIKGFRDAAEEGGTAVTGGQTVVNPWIIIGGVATVVCQPNEFILPDSAVVGDVLVLTKPLGTQVAVNAHQWLDNPERWNKIKMVVSREEVELGYQEAMFNMATLNRIAAGLMHTFNAHAATDITGFGILGHSQNLAKQQRNEVSFVIHNLPIIAKMAAISKASGRFGLLQGTSAETSGGLLICLPREQAARFCSEIKSSKYGEGHQAWIVGIVEKGNRTARIIDKPRVIEVLPRGATATPLVPENSSASSEPSL

Target ORF information:

RefSeq Version	NM_001114732.1
Organism	Bos taurus(Cattle)
Definition	Bos taurus selenophosphate synthetase 2 (SEPHS2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001114732.1

ORF Insert Sequence:

ATGGCGGAAG TCGCGGCGAC TGGTGCCGGC GGAGAGGCTA TGGCGGCAGT GGCGGCGGGG 
GAAGGCTCCT CGGGCCCCGT GGGCTTGCCT CAAGGCCGGG GCTTCTCGAG CTACCGGCCC 
TTCGAGCCCC AGGCGTTGGG TCTCAGCCCG AGCTGGCGGC TCACCGGCTT CTCTGGCATG 
AAGGGCTGAG GCTGCAAGGT CCCCCAGGAG ACGCTGCTCA AACTCCTGGC GGGACTGACG 
CGACCGGAGG TGCTGCCCGC GCCGGGCCGG GGCCTGGTCG GAGGCCTTGA AGAGACGGCC 
CAGGAAGCCG GTCTGCCGGT GAGGGCGGAA CCCAGCCCTC CCTTCCCCAC CCTGGGCATT 
GGGATGGACT CCTGCGTCAT ACCCCTGAGA CACGGGGGCC TGTCCTTGGT GCAGACCACC 
GACTTCTTTT ACCCGCTGGT GGAAGATCCC TACATGATGG GGCGCATCGC ATGTGCCAAC 
GTGCTGAGTG ACCTTTACGC GATGGGCATT ACTGAGTGTG ACAACATGTT GATGCTACTC 
AGCGTCAGCC AGAGTATGCC TGAGGAGGAG CGAGAAAAGA TAACACCACT CATGATCAAA 
GGCTTTCGAG ATGCTGCCGA GGAAGGAGGG ACTGCAGTGA CTGGTGGGCA AACGGTGGTA 
AACCCTTGGA TTATCATCGG TGGAGTTGCC ACTGTAGTAT GTCAACCAAA TGAATTCATC 
CTGCCTGACA GTGCCGTTGT TGGGGATGTG CTCGTGTTAA CTAAACCCTT AGGAACTCAG 
GTTGCCGTCA ATGCCCACCA ATGGCTGGAT AATCCTGAAC GATGGAATAA GATAAAGATG 
GTGGTCTCCA GAGAAGAGGT AGAGCTGGGT TATCAGGAAG CCATGTTCAA CATGGCTACC 
CTCAATAGAA TTGCTGCCGG GCTGATGCAC ACGTTTAATG CCCATGCAGC CACAGATATC 
ACTGGCTTTG GCATTCTGGG TCACTCTCAG AACCTTGCAA AACAACAAAG AAATGAGGTC 
TCCTTTGTCA TTCATAATCT GCCCATCATT GCCAAGATGG CTGCCATCAG CAAGGCCAGC 
GGGCGCTTCG GTCTCCTGCA AGGAACCTCA GCAGAAACTT CTGGGGGGCT ACTAATTTGT 
CTGCCAAGAG AACAGGCAGC TCGCTTTTGT TCAGAAATCA AATCTTCAAA GTATGGAGAG 
GGTCATCAAG CCTGGATCGT CGGTATTGTG GAAAAGGGAA ACCGGACAGC CCGGATCATT 
GACAAGCCTC GCGTTATTGA GGTCCTACCT CGTGGGGCCA CTGCTACTCC TCTTGTTCCT 
GAGAACTCCA GTGCCTCCTC TGAGCCTAGC TTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEPHS2 ( NM_001114732.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001108204.2 Bos taurus selenophosphate synthetase 2 (SEPHS2), mRNA.

Price & Availability↑

CloneID	OBa216292
Clone ID Related Accession (Same CDS sequence)	NM_001114732.2
Accession Version	NM_001114732.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1356bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-22
Organism	Bos taurus(cattle)
Product	selenide, water dikinase 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EV613891.1, EE381814.1, EE935132.1, EV733339.1 and CN439041.1. On May 15, 2017 this sequence version replaced NM_001114732.1. ##RefSeq-Attributes-START## protein contains selenocysteine :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGAAG TCGCGGCGAC TGGTGCCGGC GGAGAGGCTA TGGCGGCAGT GGCGGCGGGG 
GAAGGCTCCT CGGGCCCCGT GGGCTTGCCT CTAGGCCGGG GCTTCTCGAG CTACCGGCCC 
TTCGAGCCCC AGGCGTTGGG TCTCAGCCCG AGCTGGCGGC TCACCGGCTT CTCTGGCATG 
AAGGGCTGAG GCTGCAAGGT CCCCCAGGAG ACGCTGCTCA AACTCCTGGC GGGACTGACG 
CGACCGGAGG TGCTGCCCGC GCCGGGCCGG GGCCTGGTCG GAGGCCTTGA AGAGACGGCC 
CAGGAAGCCG GTCTGCCGGT GAGGGCGGAA CCCAGCCCTC CCTTCCCCAC CCTGGGCATT 
GGGATGGACT CCTGCGTCAT ACCCCTGAGA CACGGGGGCC TGTCCTTGGT GCAGACCACC 
GACTTCTTTT ACCCGCTGGT GGAAGATCCC TACATGATGG GGCGCATCGC ATGTGCCAAC 
GTGCTGAGTG ACCTTTACGC GATGGGCATT ACTGAGTGTG ACAACATGTT GATGCTACTC 
AGCGTCAGCC AGAGTATGCC TGAGGAGGAG CGAGAAAAGA TAACACCACT CATGATCAAA 
GGCTTTCGAG ATGCTGCCGA GGAAGGAGGG ACTGCAGTGA CTGGTGGGCA AACGGTGGTA 
AACCCTTGGA TTATCATCGG TGGAGTTGCC ACTGTAGTAT GTCAACCAAA TGAATTCATC 
CTGCCTGACA GTGCCGTTGT TGGGGATGTG CTCGTGTTAA CTAAACCCTT AGGAACTCAG 
GTTGCCGTCA ATGCCCACCA ATGGCTGGAT AATCCTGAAC GATGGAATAA GATAAAGATG 
GTGGTCTCCA GAGAAGAGGT AGAGCTGGGT TATCAGGAAG CCATGTTCAA CATGGCTACC 
CTCAATAGAA TTGCTGCCGG GCTGATGCAC ACGTTTAATG CCCATGCAGC CACAGATATC 
ACTGGCTTTG GCATTCTGGG TCACTCTCAG AACCTTGCAA AACAACAAAG AAATGAGGTC 
TCCTTTGTCA TTCATAATCT GCCCATCATT GCCAAGATGG CTGCCATCAG CAAGGCCAGC 
GGGCGCTTCG GTCTCCTGCA AGGAACCTCA GCAGAAACTT CTGGGGGGCT ACTAATTTGT 
CTGCCAAGAG AACAGGCAGC TCGCTTTTGT TCAGAAATCA AATCTTCAAA GTATGGAGAG 
GGTCATCAAG CCTGGATCGT CGGTATTGTG GAAAAGGGAA ACCGGACAGC CCGGATCATT 
GACAAGCCTC GCGTTATTGA GGTCCTACCT CGTGGGGCCA CTGCTACTCC TCTTGTTCCT 
GAGAACTCCA GTGCCTCCTC TGAGCCTAGC TTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001108204.2
CDS	207..1562
Translation	MAEVAATGAGGEAMAAVAAGEGSSGPVGLPLGRGFSSYRPFEPQALGLSPSWRLTGFSGMKG*GCKVPQETLLKLLAGLTRPEVLPAPGRGLVGGLEETAQEAGLPVRAEPSPPFPTLGIGMDSCVIPLRHGGLSLVQTTDFFYPLVEDPYMMGRIACANVLSDLYAMGITECDNMLMLLSVSQSMPEEEREKITPLMIKGFRDAAEEGGTAVTGGQTVVNPWIIIGGVATVVCQPNEFILPDSAVVGDVLVLTKPLGTQVAVNAHQWLDNPERWNKIKMVVSREEVELGYQEAMFNMATLNRIAAGLMHTFNAHAATDITGFGILGHSQNLAKQQRNEVSFVIHNLPIIAKMAAISKASGRFGLLQGTSAETSGGLLICLPREQAARFCSEIKSSKYGEGHQAWIVGIVEKGNRTARIIDKPRVIEVLPRGATATPLVPENSSASSEPSL

Target ORF information:

RefSeq Version	NM_001114732.2
Organism	Bos taurus(cattle)
Definition	Bos taurus selenophosphate synthetase 2 (SEPHS2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001114732.2

ORF Insert Sequence:

ATGGCGGAAG TCGCGGCGAC TGGTGCCGGC GGAGAGGCTA TGGCGGCAGT GGCGGCGGGG 
GAAGGCTCCT CGGGCCCCGT GGGCTTGCCT CTAGGCCGGG GCTTCTCGAG CTACCGGCCC 
TTCGAGCCCC AGGCGTTGGG TCTCAGCCCG AGCTGGCGGC TCACCGGCTT CTCTGGCATG 
AAGGGCTGAG GCTGCAAGGT CCCCCAGGAG ACGCTGCTCA AACTCCTGGC GGGACTGACG 
CGACCGGAGG TGCTGCCCGC GCCGGGCCGG GGCCTGGTCG GAGGCCTTGA AGAGACGGCC 
CAGGAAGCCG GTCTGCCGGT GAGGGCGGAA CCCAGCCCTC CCTTCCCCAC CCTGGGCATT 
GGGATGGACT CCTGCGTCAT ACCCCTGAGA CACGGGGGCC TGTCCTTGGT GCAGACCACC 
GACTTCTTTT ACCCGCTGGT GGAAGATCCC TACATGATGG GGCGCATCGC ATGTGCCAAC 
GTGCTGAGTG ACCTTTACGC GATGGGCATT ACTGAGTGTG ACAACATGTT GATGCTACTC 
AGCGTCAGCC AGAGTATGCC TGAGGAGGAG CGAGAAAAGA TAACACCACT CATGATCAAA 
GGCTTTCGAG ATGCTGCCGA GGAAGGAGGG ACTGCAGTGA CTGGTGGGCA AACGGTGGTA 
AACCCTTGGA TTATCATCGG TGGAGTTGCC ACTGTAGTAT GTCAACCAAA TGAATTCATC 
CTGCCTGACA GTGCCGTTGT TGGGGATGTG CTCGTGTTAA CTAAACCCTT AGGAACTCAG 
GTTGCCGTCA ATGCCCACCA ATGGCTGGAT AATCCTGAAC GATGGAATAA GATAAAGATG 
GTGGTCTCCA GAGAAGAGGT AGAGCTGGGT TATCAGGAAG CCATGTTCAA CATGGCTACC 
CTCAATAGAA TTGCTGCCGG GCTGATGCAC ACGTTTAATG CCCATGCAGC CACAGATATC 
ACTGGCTTTG GCATTCTGGG TCACTCTCAG AACCTTGCAA AACAACAAAG AAATGAGGTC 
TCCTTTGTCA TTCATAATCT GCCCATCATT GCCAAGATGG CTGCCATCAG CAAGGCCAGC 
GGGCGCTTCG GTCTCCTGCA AGGAACCTCA GCAGAAACTT CTGGGGGGCT ACTAATTTGT 
CTGCCAAGAG AACAGGCAGC TCGCTTTTGT TCAGAAATCA AATCTTCAAA GTATGGAGAG 
GGTCATCAAG CCTGGATCGT CGGTATTGTG GAAAAGGGAA ACCGGACAGC CCGGATCATT 
GACAAGCCTC GCGTTATTGA GGTCCTACCT CGTGGGGCCA CTGCTACTCC TCTTGTTCCT 
GAGAACTCCA GTGCCTCCTC TGAGCCTAGC TTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEPHS2 ( NM_001114732.3 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001108204.2 Bos taurus selenophosphate synthetase 2 (SEPHS2), mRNA.

Price & Availability↑

CloneID	OBa239160
Clone ID Related Accession (Same CDS sequence)	NM_001114732.3
Accession Version	NM_001114732.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1356bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-11
Organism	Bos taurus(cattle)
Product	selenide, water dikinase 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EV613891.1, DV810180.1, BE478511.1, EV737849.1, EV741327.1 and CN439041.1. On Jun 20, 2018 this sequence version replaced NM_001114732.2. ##RefSeq-Attributes-START## protein contains selenocysteine :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGAAG TCGCGGCGAC TGGTGCCGGC GGAGAAGCTA TGGCGGCAGT GGCGGCGGGG 
GAAGGCTCCT CGGGCCCCGT GGGCTTGCCT CTAGGCCGGG GCTTCTCGAG CTACCGGCCC 
TTCGAGCCCC AGGCGTTGGG TCTCAGCCCG AGCTGGCGGC TCACCGGCTT CTCTGGCATG 
AAGGGCTGAG GCTGCAAGGT CCCCCAGGAG ACGCTGCTCA AACTCCTGGC GGGACTGACG 
CGACCGGAGG TGCTGCCCGC GCCGGGCCGG GGCCTGGTCG GAGGCCTTGA AGAGACGGCC 
CAGGAAGCCG GTCTGCCGGT GAGGGCGGAA CCCAGCCCTC CCTTCCCCAC CCTGGGCATT 
GGGATGGACT CCTGCGTCAT ACCCCTGAGA CACGGGGGCC TGTCCTTGGT GCAGACCACC 
GACTTCTTTT ACCCGCTGGT GGAAGATCCC TACATGATGG GGCGCATCGC ATGTGCCAAC 
GTGCTGAGTG ACCTTTACGC GATGGGCATT ACTGAGTGTG ACAACATGTT GATGCTACTC 
AGCGTCAGCC AGAGTATGCC TGAGGAGGAG CGAGAAAAGA TAACACCACT CATGATCAAA 
GGCTTTCGAG ATGCTGCCGA GGAAGGAGGG ACTGCAGTGA CTGGTGGGCA AACGGTGGTA 
AACCCTTGGA TTATCATCGG TGGAGTTGCC ACTGTAGTAT GTCAACCAAA TGAATTCATC 
CTGCCTGACA GTGCCGTTGT TGGGGATGTG CTCGTGTTAA CTAAACCCTT AGGAACTCAG 
GTTGCCGTCA ATGCCCACCA ATGGCTGGAT AATCCTGAAC GATGGAATAA GATAAAGATG 
GTGGTCTCCA GAGAAGAGGT AGAGCTGGGT TATCAGGAAG CCATGTTCAA CATGGCTACC 
CTCAATAGAA TTGCTGCCGG GCTGATGCAC ACGTTTAATG CCCATGCAGC CACAGATATC 
ACTGGCTTTG GCATTCTGGG TCACTCTCAG AACCTTGCAA AACAACAAAG AAATGAGGTC 
TCCTTTGTCA TTCATAATCT GCCCATCATT GCCAAGATGG CTGCCATCAG CAAGGCCAGC 
GGGCGCTTCG GTCTCCTGCA AGGAACCTCA GCAGAAACTT CTGGGGGGCT ACTAATTTGT 
CTGCCAAGAG AACAGGCAGC TCGCTTTTGT TCAGAAATCA AATCTTCAAA GTATGGAGAG 
GGTCATCAAG CCTGGATCGT CGGTATTGTG GAAAAGGGAA ACCGGACAGC CCGGATCATT 
GACAAGCCTC GCGTTATTGA GGTCCTACCT CGTGGGGCCA CTGCTACTCC TCTTGTTCCT 
GAGAACTCCA GTGCCTCCTC TGAGCCTAGC TTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001108204.2
CDS	207..1562
Translation	MAEVAATGAGGEAMAAVAAGEGSSGPVGLPLGRGFSSYRPFEPQALGLSPSWRLTGFSGMKG*GCKVPQETLLKLLAGLTRPEVLPAPGRGLVGGLEETAQEAGLPVRAEPSPPFPTLGIGMDSCVIPLRHGGLSLVQTTDFFYPLVEDPYMMGRIACANVLSDLYAMGITECDNMLMLLSVSQSMPEEEREKITPLMIKGFRDAAEEGGTAVTGGQTVVNPWIIIGGVATVVCQPNEFILPDSAVVGDVLVLTKPLGTQVAVNAHQWLDNPERWNKIKMVVSREEVELGYQEAMFNMATLNRIAAGLMHTFNAHAATDITGFGILGHSQNLAKQQRNEVSFVIHNLPIIAKMAAISKASGRFGLLQGTSAETSGGLLICLPREQAARFCSEIKSSKYGEGHQAWIVGIVEKGNRTARIIDKPRVIEVLPRGATATPLVPENSSASSEPSL

Target ORF information:

RefSeq Version	NM_001114732.3
Organism	Bos taurus(cattle)
Definition	Bos taurus selenophosphate synthetase 2 (SEPHS2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001114732.3

ORF Insert Sequence:

ATGGCGGAAG TCGCGGCGAC TGGTGCCGGC GGAGAAGCTA TGGCGGCAGT GGCGGCGGGG 
GAAGGCTCCT CGGGCCCCGT GGGCTTGCCT CTAGGCCGGG GCTTCTCGAG CTACCGGCCC 
TTCGAGCCCC AGGCGTTGGG TCTCAGCCCG AGCTGGCGGC TCACCGGCTT CTCTGGCATG 
AAGGGCTGAG GCTGCAAGGT CCCCCAGGAG ACGCTGCTCA AACTCCTGGC GGGACTGACG 
CGACCGGAGG TGCTGCCCGC GCCGGGCCGG GGCCTGGTCG GAGGCCTTGA AGAGACGGCC 
CAGGAAGCCG GTCTGCCGGT GAGGGCGGAA CCCAGCCCTC CCTTCCCCAC CCTGGGCATT 
GGGATGGACT CCTGCGTCAT ACCCCTGAGA CACGGGGGCC TGTCCTTGGT GCAGACCACC 
GACTTCTTTT ACCCGCTGGT GGAAGATCCC TACATGATGG GGCGCATCGC ATGTGCCAAC 
GTGCTGAGTG ACCTTTACGC GATGGGCATT ACTGAGTGTG ACAACATGTT GATGCTACTC 
AGCGTCAGCC AGAGTATGCC TGAGGAGGAG CGAGAAAAGA TAACACCACT CATGATCAAA 
GGCTTTCGAG ATGCTGCCGA GGAAGGAGGG ACTGCAGTGA CTGGTGGGCA AACGGTGGTA 
AACCCTTGGA TTATCATCGG TGGAGTTGCC ACTGTAGTAT GTCAACCAAA TGAATTCATC 
CTGCCTGACA GTGCCGTTGT TGGGGATGTG CTCGTGTTAA CTAAACCCTT AGGAACTCAG 
GTTGCCGTCA ATGCCCACCA ATGGCTGGAT AATCCTGAAC GATGGAATAA GATAAAGATG 
GTGGTCTCCA GAGAAGAGGT AGAGCTGGGT TATCAGGAAG CCATGTTCAA CATGGCTACC 
CTCAATAGAA TTGCTGCCGG GCTGATGCAC ACGTTTAATG CCCATGCAGC CACAGATATC 
ACTGGCTTTG GCATTCTGGG TCACTCTCAG AACCTTGCAA AACAACAAAG AAATGAGGTC 
TCCTTTGTCA TTCATAATCT GCCCATCATT GCCAAGATGG CTGCCATCAG CAAGGCCAGC 
GGGCGCTTCG GTCTCCTGCA AGGAACCTCA GCAGAAACTT CTGGGGGGCT ACTAATTTGT 
CTGCCAAGAG AACAGGCAGC TCGCTTTTGT TCAGAAATCA AATCTTCAAA GTATGGAGAG 
GGTCATCAAG CCTGGATCGT CGGTATTGTG GAAAAGGGAA ACCGGACAGC CCGGATCATT 
GACAAGCCTC GCGTTATTGA GGTCCTACCT CGTGGGGCCA CTGCTACTCC TCTTGTTCCT 
GAGAACTCCA GTGCCTCCTC TGAGCCTAGC TTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Selenoprotein Gene Nomenclature.
The Journal of biological chemistry291(46)24036-24040(2016 Nov)
Gladyshev VN,Arn?r ES,Berry MJ,Brigelius-Floh? R,Bruford EA,Burk RF,Carlson BA,Castellano S,Chavatte L,Conrad M,Copeland PR,Diamond AM,Driscoll DM,Ferreiro A,Floh? L,Green FR,Guig? R,Handy DE,Hatfield DL,Hesketh J,Hoffmann PR,Holmgren A,Hondal RJ,Howard MT,Huang K,Kim HY,Kim IY,K?hrle J,Krol A,Kryukov GV,Lee BJ,Lee BC,Lei XG,Liu Q,Lescure A,Lobanov AV,Loscalzo J,Maiorino M,Mariotti M,Sandeep Prabhu K,Rayman MP,Rozovsky S,Salinas G,Schmidt EE,Schomburg L,Schweizer U,Simonovi? M,Sunde RA,Tsuji PA,Tweedie S,Ursini F,Whanger PD,Zhang Y

Composition and evolution of the vertebrate and mammalian selenoproteomes.
PloS one7(3)e33066(2012)
Mariotti M,Ridge PG,Zhang Y,Lobanov AV,Pringle TH,Guigo R,Hatfield DL,Gladyshev VN

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Active bovine selenophosphate synthetase 2, not having selenocysteine.
Molecular biology reports35(4)541-9(2008 Dec)
Furumiya K,Kanaya K,Tanabe K,Tanaka Y,Mizutani T

Analysis of bovine mammary gland EST and functional annotation of the Bos taurus gene index.
Mammalian genome : official journal of the International Mammalian Genome Society13(7)373-9(2002 Jul)
Sonstegard TS,Capuco AV,White J,Van Tassell CP,Connor EE,Cho J,Sultana R,Shade L,Wray JE,Wells KD,Quackenbush J

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SEPHS2 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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