Sephs2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sephs2
Entrez Gene ID	20768
Full Name	selenophosphate synthetase 2
Synonyms	Sps2,Ysg3
General protein information	Preferred Names selenophosphate synthetase 2 Names selenide, water dikinase 2 selenium donor protein 2 selenophosphate synthase 2 the SelD gene product
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7 F3\|7 69.43 cM
Summary	This gene encodes an enzyme that catalyzes the production of monoselenophosphate (MSP) from selenide and ATP. MSP is the selenium donor required for synthesis of selenocysteine (Sec), which is co-translationally incorporated into selenoproteins at in-frame UGA codons that normally signal translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. This protein is itself a selenoprotein containing a Sec residue at its active site, suggesting the existence of an autoregulatory mechanism. It is preferentially expressed in tissues implicated in the synthesis of selenoproteins and in sites of blood cell development. [provided by RefSeq, May 2017].

mRNA and Protein(s)

mRNA	Protein	Name
NM_009266.3	NP_033292.2	selenide, water dikinase 2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Sephs2	NP_033292.2
Bos taurus (cattle)	SEPHS2	NP_001108204.1
Rattus norvegicus (Norway rat)	Sephs2	NP_001073358.2
Xenopus tropicalis (tropical clawed frog)	sephs2	NP_001072895.2
Homo sapiens (human)	SEPHS2	NP_036380.2
Pan troglodytes (chimpanzee)	SEPHS2	NP_001108203.1
	SEPHS2	NP_001152892.1
Canis lupus familiaris (dog)	SEPHS2	NP_001108208.1
Danio rerio (zebrafish)	sps2	NP_001004295.2

Gene Ontology
Bibliography

Related articles in PubMed

Selenoprotein Gene Nomenclature.
Gladyshev VN, Arn?r ES, Berry MJ, Brigelius-Floh? R, Bruford EA, Burk RF, Carlson BA, Castellano S, Chavatte L, Conrad M, Copeland PR, Diamond AM, Driscoll DM, Ferreiro A, Floh? L, Green FR, Guig? R, Handy DE, Hatfield DL, Hesketh J, Hoffmann PR, Holmgren A, Hondal RJ, Howard MT, Huang K, Kim HY, Kim IY, K?hrle J, Krol A, Kryukov GV, Lee BJ, Lee BC, Lei XG, Liu Q, Lescure A, Lobanov AV, Loscalzo J, Maiorino M, Mariotti M, Sandeep Prabhu K, Rayman MP, Rozovsky S, Salinas G, Schmidt EE, Schomburg L, Schweizer U, Simonovi? M, Sunde RA, Tsuji PA, Tweedie S, Ursini F, Whanger PD, Zhang Y
The Journal of biological chemistry291(46)24036-24040(2016 Nov)

Selenophosphate synthetase 2 is essential for selenoprotein biosynthesis.
Xu XM, Carlson BA, Irons R, Mix H, Zhong N, Gladyshev VN, Hatfield DL
The Biochemical journal404(1)115-20(2007 May)

Fetal mouse selenophosphate synthetase 2 (SPS2): characterization of the cysteine mutant form overproduced in a baculovirus-insect cell system.
Kim IY, Guimar?es MJ, Zlotnik A, Bazan JF, Stadtman TC
Proceedings of the National Academy of Sciences of the United States of America94(2)418-21(1997 Jan)

Identification of a novel selD homolog from eukaryotes, bacteria, and archaea: is there an autoregulatory mechanism in selenocysteine metabolism?
Guimar?es MJ, Peterson D, Vicari A, Cocks BG, Copeland NG, Gilbert DJ, Jenkins NA, Ferrick DA, Kastelein RA, Bazan JF, Zlotnik A
Proceedings of the National Academy of Sciences of the United States of America93(26)15086-91(1996 Dec)

A new approach to the study of haematopoietic development in the yolk sac and embryoid bodies.
Guimar?es MJ, Bazan JF, Zlotnik A, Wiles MV, Grimaldi JC, Lee F, McClanahan T
Development (Cambridge, England)121(10)3335-46(1995 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These in vivo studies indicate that SPS2 is essential for generating the selenium donor for selenocysteine biosynthesis in mammals.
Title: Selenophosphate synthetase 2 is essential for selenoprotein biosynthesis.

The following Sephs2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sephs2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu58224	NM_009266.3 Latest version!	Mus musculus selenophosphate synthetase 2 (Sephs2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$265.30 $379.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sephs2 ( NM_009266.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033292.2 Mus musculus selenophosphate synthetase 2 (Sephs2), mRNA.

Price & Availability↑

CloneID	OMu58224
Clone ID Related Accession (Same CDS sequence)	NM_009266.3
Accession Version	NM_009266.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Mus musculus(house mouse)
Product	selenide, water dikinase 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK168722.1, AK149516.1 and U43285.1. On Apr 6, 2007 this sequence version replaced NM_009266.2. ##Evidence-Data-START## Transcript is intronless :: U43285.1, BC016508.1 [ECO:0000345] ##Evidence-Data-END## ##RefSeq-Attributes-START## protein contains selenocysteine :: PMID: 8986768 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGAAG CGGCGGCGGC GGGCGCCAGC GGAGAAACCA TGGCGGCGCT AGTGGCCGCA 
GAAGGTTCCT TGGGCCCGGC GGGCTGGTCT GCCGGCCGGA GTTTCTCCAA CTACCGGCCG 
TTCGAGCCCC AGACACTGGG CTTCAGCCCG AGCTGGCGGC TGACGAGCTT CTCCGGCATG 
AAGGGCTGAG GCTGCAAGGT CCCCCAGGAG ACCCTGCTCA AACTCCTGGA GGGACTGACG 
CGGCCCGCGC TGCAGCCCCC GCTTACCTCG GGTCTGGTCG GGGGCCAGGA AGAGACGGTG 
CAGGAAGGGG GCCTGTCCAC CAGGCCCGGC CCCGGCTCAG CCTTCCCCTC GCTGAGCATT 
GGCATGGACT CCTGCGTCAT CCCCCTGAGG CACGGAGGCC TGTCGCTGGT GCAGACCACC 
GACTTCTTTT ACCCCTTGGT GGAAGATCCC TATATGATGG GGCGCATAGC TTGTGCCAAT 
GTGCTCAGTG ACCTCTATGC CATGGGTATC ACTGAGTGTG ACAACATGTT GATGTTACTC 
AGTGTGAGCC AGAGCATGAG TGAAAAGGAA CGAGAGAAGG TGACACCGCT CATGATCAAA 
GGCTTTCGTG ACGCTGCGGA GGAGGGAGGC ACTGCAGTGA CCGGTGGACA GACAGTGGTC 
AACCCGTGGA TTATCATCGG TGGCGTTGCC ACGGTGGTGT GTCAGCAAAA TGAATTCATA 
ATGCCTGATA GTGCCGTGGT AGGAGATGTG CTGGTATTAA CCAAACCTTT AGGAACCCAG 
GTTGCTGCCA ATGCCCACCA ATGGCTGGAT AATCCTGAGA AATGGAATAA AATCAAGATG 
GTGGTTTCCA GAGAGGAAGT AGAGTTAGCC TATCAGGAAG CTATGTTCAA TATGGCTACT 
CTAAACAGGA CTGCTGCTGG CTTGATGCAC ACTTTTAATG CCCACGCAGC CACGGATATC 
ACAGGCTTTG GCATATTAGG ACACTCTCAG AACCTGGCAA AACAGCAAAA AAATGAAGTG 
TCCTTTGTCA TACATAATCT GCCAATAATT GCCAAGATGG CTGCGATCAG CAAAGCCAGT 
GGGCGCTTTG GCCTCCTCCA AGGAACGTCA GCTGAAACCT CTGGGGGATT ACTGATTTGT 
CTGCCAAGAG AGCAGGCGGC CCGCTTTTGT TCGGAAATCA AATCTTCCAA GTACGGAGAG 
GGTCACCAAG CTTGGATCGT TGGCATCGTG GAGAAGGGAA ACCGGACAGC CCGGATCATT 
GACAAGCCTC GCGTTATTGA AGTTCTACCT CGGGGAGCCT CTGCTGCTGC TGCTGCTGCT 
CCTGATAATT CCAACGCAGC CTCTGAGCCT AGTTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033292.2
CDS	141..1499
Translation	MAEAAAAGASGETMAALVAAEGSLGPAGWSAGRSFSNYRPFEPQTLGFSPSWRLTSFSGMKG*GCKVPQETLLKLLEGLTRPALQPPLTSGLVGGQEETVQEGGLSTRPGPGSAFPSLSIGMDSCVIPLRHGGLSLVQTTDFFYPLVEDPYMMGRIACANVLSDLYAMGITECDNMLMLLSVSQSMSEKEREKVTPLMIKGFRDAAEEGGTAVTGGQTVVNPWIIIGGVATVVCQQNEFIMPDSAVVGDVLVLTKPLGTQVAANAHQWLDNPEKWNKIKMVVSREEVELAYQEAMFNMATLNRTAAGLMHTFNAHAATDITGFGILGHSQNLAKQQKNEVSFVIHNLPIIAKMAAISKASGRFGLLQGTSAETSGGLLICLPREQAARFCSEIKSSKYGEGHQAWIVGIVEKGNRTARIIDKPRVIEVLPRGASAAAAAAPDNSNAASEPSS

Target ORF information:

RefSeq Version	NM_009266.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus selenophosphate synthetase 2 (Sephs2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009266.3

ORF Insert Sequence:

ATGGCGGAAG CGGCGGCGGC GGGCGCCAGC GGAGAAACCA TGGCGGCGCT AGTGGCCGCA 
GAAGGTTCCT TGGGCCCGGC GGGCTGGTCT GCCGGCCGGA GTTTCTCCAA CTACCGGCCG 
TTCGAGCCCC AGACACTGGG CTTCAGCCCG AGCTGGCGGC TGACGAGCTT CTCCGGCATG 
AAGGGCTGAG GCTGCAAGGT CCCCCAGGAG ACCCTGCTCA AACTCCTGGA GGGACTGACG 
CGGCCCGCGC TGCAGCCCCC GCTTACCTCG GGTCTGGTCG GGGGCCAGGA AGAGACGGTG 
CAGGAAGGGG GCCTGTCCAC CAGGCCCGGC CCCGGCTCAG CCTTCCCCTC GCTGAGCATT 
GGCATGGACT CCTGCGTCAT CCCCCTGAGG CACGGAGGCC TGTCGCTGGT GCAGACCACC 
GACTTCTTTT ACCCCTTGGT GGAAGATCCC TATATGATGG GGCGCATAGC TTGTGCCAAT 
GTGCTCAGTG ACCTCTATGC CATGGGTATC ACTGAGTGTG ACAACATGTT GATGTTACTC 
AGTGTGAGCC AGAGCATGAG TGAAAAGGAA CGAGAGAAGG TGACACCGCT CATGATCAAA 
GGCTTTCGTG ACGCTGCGGA GGAGGGAGGC ACTGCAGTGA CCGGTGGACA GACAGTGGTC 
AACCCGTGGA TTATCATCGG TGGCGTTGCC ACGGTGGTGT GTCAGCAAAA TGAATTCATA 
ATGCCTGATA GTGCCGTGGT AGGAGATGTG CTGGTATTAA CCAAACCTTT AGGAACCCAG 
GTTGCTGCCA ATGCCCACCA ATGGCTGGAT AATCCTGAGA AATGGAATAA AATCAAGATG 
GTGGTTTCCA GAGAGGAAGT AGAGTTAGCC TATCAGGAAG CTATGTTCAA TATGGCTACT 
CTAAACAGGA CTGCTGCTGG CTTGATGCAC ACTTTTAATG CCCACGCAGC CACGGATATC 
ACAGGCTTTG GCATATTAGG ACACTCTCAG AACCTGGCAA AACAGCAAAA AAATGAAGTG 
TCCTTTGTCA TACATAATCT GCCAATAATT GCCAAGATGG CTGCGATCAG CAAAGCCAGT 
GGGCGCTTTG GCCTCCTCCA AGGAACGTCA GCTGAAACCT CTGGGGGATT ACTGATTTGT 
CTGCCAAGAG AGCAGGCGGC CCGCTTTTGT TCGGAAATCA AATCTTCCAA GTACGGAGAG 
GGTCACCAAG CTTGGATCGT TGGCATCGTG GAGAAGGGAA ACCGGACAGC CCGGATCATT 
GACAAGCCTC GCGTTATTGA AGTTCTACCT CGGGGAGCCT CTGCTGCTGC TGCTGCTGCT 
CCTGATAATT CCAACGCAGC CTCTGAGCCT AGTTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Selenoprotein Gene Nomenclature.
The Journal of biological chemistry291(46)24036-24040(2016 Nov)
Gladyshev VN,Arn?r ES,Berry MJ,Brigelius-Floh? R,Bruford EA,Burk RF,Carlson BA,Castellano S,Chavatte L,Conrad M,Copeland PR,Diamond AM,Driscoll DM,Ferreiro A,Floh? L,Green FR,Guig? R,Handy DE,Hatfield DL,Hesketh J,Hoffmann PR,Holmgren A,Hondal RJ,Howard MT,Huang K,Kim HY,Kim IY,K?hrle J,Krol A,Kryukov GV,Lee BJ,Lee BC,Lei XG,Liu Q,Lescure A,Lobanov AV,Loscalzo J,Maiorino M,Mariotti M,Sandeep Prabhu K,Rayman MP,Rozovsky S,Salinas G,Schmidt EE,Schomburg L,Schweizer U,Simonovi? M,Sunde RA,Tsuji PA,Tweedie S,Ursini F,Whanger PD,Zhang Y

Selenophosphate synthetase 2 is essential for selenoprotein biosynthesis.
The Biochemical journal404(1)115-20(2007 May)
Xu XM,Carlson BA,Irons R,Mix H,Zhong N,Gladyshev VN,Hatfield DL

Fetal mouse selenophosphate synthetase 2 (SPS2): characterization of the cysteine mutant form overproduced in a baculovirus-insect cell system.
Proceedings of the National Academy of Sciences of the United States of America94(2)418-21(1997 Jan)
Kim IY,Guimar?es MJ,Zlotnik A,Bazan JF,Stadtman TC

Identification of a novel selD homolog from eukaryotes, bacteria, and archaea: is there an autoregulatory mechanism in selenocysteine metabolism?
Proceedings of the National Academy of Sciences of the United States of America93(26)15086-91(1996 Dec)
Guimar?es MJ,Peterson D,Vicari A,Cocks BG,Copeland NG,Gilbert DJ,Jenkins NA,Ferrick DA,Kastelein RA,Bazan JF,Zlotnik A

A new approach to the study of haematopoietic development in the yolk sac and embryoid bodies.
Development (Cambridge, England)121(10)3335-46(1995 Oct)
Guimar?es MJ,Bazan JF,Zlotnik A,Wiles MV,Grimaldi JC,Lee F,McClanahan T

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Sephs2 cDNA ORF clone, Mus musculus(house mouse)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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