ITSN1 cDNA ORF clone, Bos taurus(cattle)

The following ITSN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ITSN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa219037 XM_024993605.1
Latest version!
Bos taurus intersectin 1 (ITSN1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa47837 XM_024993604.1
Latest version!
Bos taurus intersectin 1 (ITSN1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa02401 XM_024993592.1
Latest version!
Bos taurus intersectin 1 (ITSN1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa02401 XM_024993593.1
Latest version!
Bos taurus intersectin 1 (ITSN1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa02403 XM_024993603.1
Latest version!
Bos taurus intersectin 1 (ITSN1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa47836 XM_024993595.1
Latest version!
Bos taurus intersectin 1 (ITSN1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa87631 XM_015461917.1
Latest version!
Bos taurus intersectin 1 (ITSN1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa87631 XM_015465187.1
Latest version!
Bos taurus intersectin 1 (ITSN1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa219037
    Clone ID Related Accession (Same CDS sequence) XM_024993605.1
    Accession Version XM_024993605.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3435bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product intersectin-1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCTCAGT TTCCCACACC CTTTGGTGGC AGCCTGGATA TTTGGGCCAT AACTGTAGAG 
    GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT
    GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAGCCTGT TTTAGCACAG
    ATATGGGCGC TCGCTGATAT GAATAATGAT GGAAGGATGG ATCAAGTGGA GTTTTCCATT
    GCTATGAAAC TCATCAAGCT GAAGCTCCAA GGATATCAGC TCCCCTCTGC ACTTCCCCCT
    GTCATGAAAC AGCAACCAGT TGCTATTTCT AGTGCACCAG CCTTTGGTAT GGGAGGTATT
    GCCAGCATGC CGCCGCTGAC CGCTGTTGCT CCTGTACCAA TGGGCTCCAT TCCCGTTGTT
    GGGATGTCTC CCCCACTAGT GTCATCTGTT CCTGCAGCAG GGGTGCCCCC CCTGGCCAAT
    GGGGCGCCCC CGGTCATACA GCCTCTGCCT GCATTTGCTC ATCCTGCTGC CACATTGCCA
    AAGAGTTCTT CCTTTAGTAG ATCTGGCCCA GGGTCGCAGT TAAACACAAA ATTACAGAAG
    GCACAATCAT TTGATGTGGC CAGTGTCCCT CCTACAGCTG AGTGGGCTGT TCCTCAGTCA
    TCCAGGCTGA AATACAGACA GCTCTTCAAT AGTCATGACA AAACCATGAG TGGACACCTA
    ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAATCAAGTT TACCACAGGC TCAGCTGGCT
    TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC
    CTGGCTATGC ACCTCATTGA TGTAGCTATG TCTGGTCAAC CACTGCCACC GGTCCTGCCC
    CCAGAATACA TTCCACCTTC CTTTAGAAGA GTTCGATCTG GTAGTGGTAT ATCTGTCATA
    ACCTCAACTT CGGTGGATCA GAGGTTGCCA GAGGAACCAG TTTTGGAAGA TGAACAGCAA
    CAACTAGAAA AGAAACTACC TGTAACCTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGG
    GGCAACCTGG AGCTGGAGAA GCGGAGACAA GCGCTCTTGG AGCAGCAGCG TAAAGAGCAG
    GAGCGCCTGG CCCAGCTGGA GCGGGCAGAG CAGGAGAGGA AGGAGCGGGA GCGCCAGGAG
    CAGGAGCGCA AAAGACAACT GGAGCTGGAA AAGCAGCTAG AGAAGCAGCG GGAGCTGGAG
    CGGCAGAGGG AGGAGGAGCG AAGGAAGGAA ATCGAGAGGC GGGAGGCTGC GAAACGGGAG
    CTTGAAAGGC AAAGACAGCT TGAATGGGAA CGGAATCGAA GACAAGAACT ATTAAATCAA
    AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT
    GAATTAGAAG CTCTAAATGA TAAAAAACAT CAATTAGAAG GAAAACTTCA GGATATCAGA
    TGTCGACTGA CAACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA
    ATTGCAGAAA TCACCCACCT ACAACAGCAA TTACAGGAGT CCCAGCAAAT GCTTGGAAGA
    CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTGAAAC AAGTTCAGCA AAACAGTTTG
    CACAGGGATT CACTTCTCAC ACTCAAAAGA GCCTTAGAAG CGAAAGAACT GGCTCGGCAG
    CAGCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GGTCAAAATT ACAGGAGATT
    GATATTTTCA ACAATCAGCT GAAGGAACTT AGAGAAATAC ATAATAAGCA GCAGCTCCAG
    AAACAGAAGT CCATAGAGGC TGAACGACTG AAACAGAAGG AACAAGAACG AAAGATCCTG
    GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CCAGCAGTGG
    CTGGAGCGCG TGCATCAGGA GGATGAGCAG CAGCGGCCAC GAAAACCCCA CGAGGAGGAG
    AAGCTCAAAA GGGAGGAGAG CGTCAAAAAG AAGGACAGTG AGGAGAAGGG CAAACCGGAA
    ATGCAAGACA AGCTGAGTCG GCTTTTCCAC CAGCATCAAG AACCAGCTAA GCCCGCTGTC
    CAGGCACCCT GGTCCACTGC AGAAAAAGGC CCACTTACGA TTTCTGCACA GGAGAATATA
    AAAGTGGTGT ATTACCGAGC ACTTTATCCC TTTGAGTCCA GGAGTCACGA TGAAATCACA
    ATCCAGCCGG GAGATATAGT CATGGTGGAC GAAAGCCAAA CTGGAGAACC GGGATGGCTT
    GGAGGAGAAT TAAAAGGAAA GACGGGATGG TTCCCTGCAA ACTACGCAGA GAAAATCCCA
    GAAAATGAGG TTCCCGCCCC AGTCAAACCA GTGACTGAGG CGGCCTCCAC CCCTACACCC
    AAAGTGGCTG TGTGTGAGAC CCCCGCCCCT TCATCCACAA CCCCTGCAGA GCCCTCCGAG
    ACCCCCAACA ACTGGGCCGA CTTCAGCTCC ACGTGGCCCA CCAGCACGAA TGAGAAACCA
    GAAACCGACA GCTGGGACGC ATGGGCAACC CAGCCCTCCC TCACCGTGCC AAGCGCCGGG
    CAGCTGAGGC AGAGGTCGGC CTTCACTCCA GCCACGGCCA CGGGCTCATC TCCGTCTCCT
    GTTCTAGGCC AGGGTGAAAA GGTGGAGGGG CTACAGGCAC AAGCCCTGTA TCCGTGGAGA
    GCCAAGAAAG ACAACCATTT AAATTTTAAC AAAAACGACG TCATCACCGT ACTGGAGCAG
    CAAGACATGT GGTGGTTTGG AGAAGTTCAA GGCCAGAAGG GTTGGTTCCC CAAGTCCTAC
    GTGAAACTCA TTTCAGGGCC CATAAGGAAA TCGACAAGCA TGGATTCTGG TCCCTCAGAA
    AGTCCTGCAA GTCTAAAGAG GGTAGCTTCC CCAGCAGCCA AGCCAGCCGT GTCGGGAGAA
    GAAATCGCGC AGGTCATTGC CTCCTACACA GCTACGGGTC CTGAACAGCT TACCCTGGCC
    CCCGGTCAGC TGATCCTGAT CCGGAAAAAG AACCCAGGTG GATGGTGGGA AGGAGAGCTA
    CAGGCACGTG GGAAAAAGCG CCAGATAGGC TGGTTTCCTG CTAATTACGT CAAACTTTTA
    AGCCCTGGGA CAAGCAAAAT CACTCCAACA GACCCACCGA AGCCAACAGC TTTCCCAACA
    GTGTGCCAGG TGATCGGGAT GTACGACTAC ACCGCGCAGA ACGACGACGA GCTGGCCTTC
    AGCAAGGGCC AGGTCATCAA TGTCCTCAAC AAGGAGGACC CCGACTGGTG GAAGGGTGAA
    GTCCACGGGC AGGTGGGGCT CTTCCCGTCC AACTACGTGA AGTTGACCAC GGACACGGAC
    CCCAGCCAGC AATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024849373.1
    CDS271..3705
    Translation

    Target ORF information:

    RefSeq Version XM_024993605.1
    Organism Bos taurus(cattle)
    Definition Bos taurus intersectin 1 (ITSN1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024993605.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCTCAGT TTCCCACACC CTTTGGTGGC AGCCTGGATA TTTGGGCCAT AACTGTAGAG 
    GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT
    GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAGCCTGT TTTAGCACAG
    ATATGGGCGC TCGCTGATAT GAATAATGAT GGAAGGATGG ATCAAGTGGA GTTTTCCATT
    GCTATGAAAC TCATCAAGCT GAAGCTCCAA GGATATCAGC TCCCCTCTGC ACTTCCCCCT
    GTCATGAAAC AGCAACCAGT TGCTATTTCT AGTGCACCAG CCTTTGGTAT GGGAGGTATT
    GCCAGCATGC CGCCGCTGAC CGCTGTTGCT CCTGTACCAA TGGGCTCCAT TCCCGTTGTT
    GGGATGTCTC CCCCACTAGT GTCATCTGTT CCTGCAGCAG GGGTGCCCCC CCTGGCCAAT
    GGGGCGCCCC CGGTCATACA GCCTCTGCCT GCATTTGCTC ATCCTGCTGC CACATTGCCA
    AAGAGTTCTT CCTTTAGTAG ATCTGGCCCA GGGTCGCAGT TAAACACAAA ATTACAGAAG
    GCACAATCAT TTGATGTGGC CAGTGTCCCT CCTACAGCTG AGTGGGCTGT TCCTCAGTCA
    TCCAGGCTGA AATACAGACA GCTCTTCAAT AGTCATGACA AAACCATGAG TGGACACCTA
    ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAATCAAGTT TACCACAGGC TCAGCTGGCT
    TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC
    CTGGCTATGC ACCTCATTGA TGTAGCTATG TCTGGTCAAC CACTGCCACC GGTCCTGCCC
    CCAGAATACA TTCCACCTTC CTTTAGAAGA GTTCGATCTG GTAGTGGTAT ATCTGTCATA
    ACCTCAACTT CGGTGGATCA GAGGTTGCCA GAGGAACCAG TTTTGGAAGA TGAACAGCAA
    CAACTAGAAA AGAAACTACC TGTAACCTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGG
    GGCAACCTGG AGCTGGAGAA GCGGAGACAA GCGCTCTTGG AGCAGCAGCG TAAAGAGCAG
    GAGCGCCTGG CCCAGCTGGA GCGGGCAGAG CAGGAGAGGA AGGAGCGGGA GCGCCAGGAG
    CAGGAGCGCA AAAGACAACT GGAGCTGGAA AAGCAGCTAG AGAAGCAGCG GGAGCTGGAG
    CGGCAGAGGG AGGAGGAGCG AAGGAAGGAA ATCGAGAGGC GGGAGGCTGC GAAACGGGAG
    CTTGAAAGGC AAAGACAGCT TGAATGGGAA CGGAATCGAA GACAAGAACT ATTAAATCAA
    AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT
    GAATTAGAAG CTCTAAATGA TAAAAAACAT CAATTAGAAG GAAAACTTCA GGATATCAGA
    TGTCGACTGA CAACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA
    ATTGCAGAAA TCACCCACCT ACAACAGCAA TTACAGGAGT CCCAGCAAAT GCTTGGAAGA
    CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTGAAAC AAGTTCAGCA AAACAGTTTG
    CACAGGGATT CACTTCTCAC ACTCAAAAGA GCCTTAGAAG CGAAAGAACT GGCTCGGCAG
    CAGCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GGTCAAAATT ACAGGAGATT
    GATATTTTCA ACAATCAGCT GAAGGAACTT AGAGAAATAC ATAATAAGCA GCAGCTCCAG
    AAACAGAAGT CCATAGAGGC TGAACGACTG AAACAGAAGG AACAAGAACG AAAGATCCTG
    GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CCAGCAGTGG
    CTGGAGCGCG TGCATCAGGA GGATGAGCAG CAGCGGCCAC GAAAACCCCA CGAGGAGGAG
    AAGCTCAAAA GGGAGGAGAG CGTCAAAAAG AAGGACAGTG AGGAGAAGGG CAAACCGGAA
    ATGCAAGACA AGCTGAGTCG GCTTTTCCAC CAGCATCAAG AACCAGCTAA GCCCGCTGTC
    CAGGCACCCT GGTCCACTGC AGAAAAAGGC CCACTTACGA TTTCTGCACA GGAGAATATA
    AAAGTGGTGT ATTACCGAGC ACTTTATCCC TTTGAGTCCA GGAGTCACGA TGAAATCACA
    ATCCAGCCGG GAGATATAGT CATGGTGGAC GAAAGCCAAA CTGGAGAACC GGGATGGCTT
    GGAGGAGAAT TAAAAGGAAA GACGGGATGG TTCCCTGCAA ACTACGCAGA GAAAATCCCA
    GAAAATGAGG TTCCCGCCCC AGTCAAACCA GTGACTGAGG CGGCCTCCAC CCCTACACCC
    AAAGTGGCTG TGTGTGAGAC CCCCGCCCCT TCATCCACAA CCCCTGCAGA GCCCTCCGAG
    ACCCCCAACA ACTGGGCCGA CTTCAGCTCC ACGTGGCCCA CCAGCACGAA TGAGAAACCA
    GAAACCGACA GCTGGGACGC ATGGGCAACC CAGCCCTCCC TCACCGTGCC AAGCGCCGGG
    CAGCTGAGGC AGAGGTCGGC CTTCACTCCA GCCACGGCCA CGGGCTCATC TCCGTCTCCT
    GTTCTAGGCC AGGGTGAAAA GGTGGAGGGG CTACAGGCAC AAGCCCTGTA TCCGTGGAGA
    GCCAAGAAAG ACAACCATTT AAATTTTAAC AAAAACGACG TCATCACCGT ACTGGAGCAG
    CAAGACATGT GGTGGTTTGG AGAAGTTCAA GGCCAGAAGG GTTGGTTCCC CAAGTCCTAC
    GTGAAACTCA TTTCAGGGCC CATAAGGAAA TCGACAAGCA TGGATTCTGG TCCCTCAGAA
    AGTCCTGCAA GTCTAAAGAG GGTAGCTTCC CCAGCAGCCA AGCCAGCCGT GTCGGGAGAA
    GAAATCGCGC AGGTCATTGC CTCCTACACA GCTACGGGTC CTGAACAGCT TACCCTGGCC
    CCCGGTCAGC TGATCCTGAT CCGGAAAAAG AACCCAGGTG GATGGTGGGA AGGAGAGCTA
    CAGGCACGTG GGAAAAAGCG CCAGATAGGC TGGTTTCCTG CTAATTACGT CAAACTTTTA
    AGCCCTGGGA CAAGCAAAAT CACTCCAACA GACCCACCGA AGCCAACAGC TTTCCCAACA
    GTGTGCCAGG TGATCGGGAT GTACGACTAC ACCGCGCAGA ACGACGACGA GCTGGCCTTC
    AGCAAGGGCC AGGTCATCAA TGTCCTCAAC AAGGAGGACC CCGACTGGTG GAAGGGTGAA
    GTCCACGGGC AGGTGGGGCT CTTCCCGTCC AACTACGTGA AGTTGACCAC GGACACGGAC
    CCCAGCCAGC AATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47837
    Clone ID Related Accession (Same CDS sequence) XM_024993604.1
    Accession Version XM_024993604.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3648bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product intersectin-1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCTCAGT TTCCCACACC CTTTGGTGGC AGCCTGGATA TTTGGGCCAT AACTGTAGAG 
    GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT
    GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAGCCTGT TTTAGCACAG
    ATATGGGCGC TCGCTGATAT GAATAATGAT GGAAGGATGG ATCAAGTGGA GTTTTCCATT
    GCTATGAAAC TCATCAAGCT GAAGCTCCAA GGATATCAGC TCCCCTCTGC ACTTCCCCCT
    GTCATGAAAC AGCAACCAGT TGCTATTTCT AGTGCACCAG CCTTTGGTAT GGGAGGTATT
    GCCAGCATGC CGCCGCTGAC CGCTGTTGCT CCTGTACCAA TGGGCTCCAT TCCCGTTGTT
    GGGATGTCTC CCCCACTAGT GTCATCTGTT CCTGCAGCAG GGGTGCCCCC CCTGGCCAAT
    GGGGCGCCCC CGGTCATACA GCCTCTGCCT GCATTTGCTC ATCCTGCTGC CACATTGCCA
    AAGAGTTCTT CCTTTAGTAG ATCTGGCCCA GGGTCGCAGT TAAACACAAA ATTACAGAAG
    GCACAATCAT TTGATGTGGC CAGTGTCCCT CCTACAGCTG AGTGGGCTGT TCCTCAGTCA
    TCCAGGCTGA AATACAGACA GCTCTTCAAT AGTCATGACA AAACCATGAG TGGACACCTA
    ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAATCAAGTT TACCACAGGC TCAGCTGGCT
    TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC
    CTGGCTATGC ACCTCATTGA TGTAGCTATG TCTGGTCAAC CACTGCCACC GGTCCTGCCC
    CCAGAATACA TTCCACCTTC CTTTAGAAGA GTTCGATCTG GTAGTGGTAT ATCTGTCATA
    ACCTCAACTT CGGTGGATCA GAGGTTGCCA GAGGAACCAG TTTTGGAAGA TGAACAGCAA
    CAACTAGAAA AGAAACTACC TGTAACCTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGG
    GGCAACCTGG AGCTGGAGAA GCGGAGACAA GCGCTCTTGG AGCAGCAGCG TAAAGAGCAG
    GAGCGCCTGG CCCAGCTGGA GCGGGCAGAG CAGGAGAGGA AGGAGCGGGA GCGCCAGGAG
    CAGGAGCGCA AAAGACAACT GGAGCTGGAA AAGCAGCTAG AGAAGCAGCG GGAGCTGGAG
    CGGCAGAGGG AGGAGGAGCG AAGGAAGGAA ATCGAGAGGC GGGAGGCTGC GAAACGGGAG
    CTTGAAAGGC AAAGACAGCT TGAATGGGAA CGGAATCGAA GACAAGAACT ATTAAATCAA
    AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT
    GAATTAGAAG CTCTAAATGA TAAAAAACAT CAATTAGAAG GAAAACTTCA GGATATCAGA
    TGTCGACTGA CAACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA
    ATTGCAGAAA TCACCCACCT ACAACAGCAA TTACAGGAGT CCCAGCAAAT GCTTGGAAGA
    CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTGAAAC AAGTTCAGCA AAACAGTTTG
    CACAGGGATT CACTTCTCAC ACTCAAAAGA GCCTTAGAAG CGAAAGAACT GGCTCGGCAG
    CAGCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GGTCAAAATT ACAGGAGATT
    GATATTTTCA ACAATCAGCT GAAGGAACTT AGAGAAATAC ATAATAAGCA GCAGCTCCAG
    AAACAGAAGT CCATAGAGGC TGAACGACTG AAACAGAAGG AACAAGAACG AAAGATCCTG
    GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CCAGCAGTGG
    CTGGAGCGCG TGCATCAGGA GGATGAGCAG CAGCGGCCAC GAAAACCCCA CGAGGAGGAG
    AAGCTCAAAA GGGAGGAGAG CGTCAAAAAG AAGGACAGTG AGGAGAAGGG CAAACCGGAA
    ATGCAAGACA AGCTGAGTCG GCTTTTCCAC CAGCATCAAG AACCAGCTAA GCCCGCTGTC
    CAGGCACCCT GGTCCACTGC AGAAAAAGGC CCACTTACGA TTTCTGCACA GGAGAATATA
    AAAGTGGTGT ATTACCGAGC ACTTTATCCC TTTGAGTCCA GGAGTCACGA TGAAATCACA
    ATCCAGCCGG GAGATATAGT CATGGTGGAC GAAAGCCAAA CTGGAGAACC GGGATGGCTT
    GGAGGAGAAT TAAAAGGAAA GACGGGATGG TTCCCTGCAA ACTACGCAGA GAAAATCCCA
    GAAAATGAGG TTCCCGCCCC AGTCAAACCA GTGACTGAGG CGGCCTCCAC CCCTACACCC
    AAAGTGGCTG TGTGTGAGAC CCCCGCCCCT TCATCCACAA CCCCTGCAGA GCCCTCCGAG
    ACCCCCAACA ACTGGGCCGA CTTCAGCTCC ACGTGGCCCA CCAGCACGAA TGAGAAACCA
    GAAACCGACA GCTGGGACGC ATGGGCAACC CAGCCCTCCC TCACCGTGCC AAGCGCCGGG
    CAGCTGAGGC AGAGGTCGGC CTTCACTCCA GCCACGGCCA CGGGCTCATC TCCGTCTCCT
    GTTCTAGGCC AGGGTGAAAA GGTGGAGGGG CTACAGGCAC AAGCCCTGTA TCCGTGGAGA
    GCCAAGAAAG ACAACCATTT AAATTTTAAC AAAAACGACG TCATCACCGT ACTGGAGCAG
    CAAGACATGT GGTGGTTTGG AGAAGTTCAA GGCCAGAAGG GTTGGTTCCC CAAGTCCTAC
    GTGAAACTCA TTTCAGGGCC CATAAGGAAA TCGACAAGCA TGGATTCTGG TCCCTCAGAA
    AGTCCTGCAA GTCTAAAGAG GGTAGCTTCC CCAGCAGCCA AGCCAGCCGT GTCGGGAGAA
    GAATTTATTG CCATGTACAC TTACGAGAGT TCTGAGCAAG GCGATTTAAC CTTTCAGCAA
    GGGGATGTGA TTTTGGTTAC CAAGAAAGAT GGTGACTGGT GGACAGGAAC AGTGGGCGAC
    AAGTCCGGCG TCTTCCCTTC TAACTATGTG AGGCTTAAAG ATTCGGAGGG CTCTGGAACT
    GCTGGGAAAA CAGGGAGTTT AGGAAAAAAA CCTGAAATCG CGCAGGTCAT TGCCTCCTAC
    ACAGCTACGG GTCCTGAACA GCTTACCCTG GCCCCCGGTC AGCTGATCCT GATCCGGAAA
    AAGAACCCAG GTGGATGGTG GGAAGGAGAG CTACAGGCAC GTGGGAAAAA GCGCCAGATA
    GGCTGGTTTC CTGCTAATTA CGTCAAACTT TTAAGCCCTG GGACAAGCAA AATCACTCCA
    ACAGACCCAC CGAAGCCAAC AGCTTTCCCA ACAGTGTGCC AGGTGATCGG GATGTACGAC
    TACACCGCGC AGAACGACGA CGAGCTGGCC TTCAGCAAGG GCCAGGTCAT CAATGTCCTC
    AACAAGGAGG ACCCCGACTG GTGGAAGGGT GAAGTCCACG GGCAGGTGGG GCTCTTCCCG
    TCCAACTACG TGAAGTTGAC CACGGACACG GACCCCAGCC AGCAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024849372.1
    CDS302..3949
    Translation

    Target ORF information:

    RefSeq Version XM_024993604.1
    Organism Bos taurus(cattle)
    Definition Bos taurus intersectin 1 (ITSN1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024993604.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCTCAGT TTCCCACACC CTTTGGTGGC AGCCTGGATA TTTGGGCCAT AACTGTAGAG 
    GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT
    GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAGCCTGT TTTAGCACAG
    ATATGGGCGC TCGCTGATAT GAATAATGAT GGAAGGATGG ATCAAGTGGA GTTTTCCATT
    GCTATGAAAC TCATCAAGCT GAAGCTCCAA GGATATCAGC TCCCCTCTGC ACTTCCCCCT
    GTCATGAAAC AGCAACCAGT TGCTATTTCT AGTGCACCAG CCTTTGGTAT GGGAGGTATT
    GCCAGCATGC CGCCGCTGAC CGCTGTTGCT CCTGTACCAA TGGGCTCCAT TCCCGTTGTT
    GGGATGTCTC CCCCACTAGT GTCATCTGTT CCTGCAGCAG GGGTGCCCCC CCTGGCCAAT
    GGGGCGCCCC CGGTCATACA GCCTCTGCCT GCATTTGCTC ATCCTGCTGC CACATTGCCA
    AAGAGTTCTT CCTTTAGTAG ATCTGGCCCA GGGTCGCAGT TAAACACAAA ATTACAGAAG
    GCACAATCAT TTGATGTGGC CAGTGTCCCT CCTACAGCTG AGTGGGCTGT TCCTCAGTCA
    TCCAGGCTGA AATACAGACA GCTCTTCAAT AGTCATGACA AAACCATGAG TGGACACCTA
    ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAATCAAGTT TACCACAGGC TCAGCTGGCT
    TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC
    CTGGCTATGC ACCTCATTGA TGTAGCTATG TCTGGTCAAC CACTGCCACC GGTCCTGCCC
    CCAGAATACA TTCCACCTTC CTTTAGAAGA GTTCGATCTG GTAGTGGTAT ATCTGTCATA
    ACCTCAACTT CGGTGGATCA GAGGTTGCCA GAGGAACCAG TTTTGGAAGA TGAACAGCAA
    CAACTAGAAA AGAAACTACC TGTAACCTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGG
    GGCAACCTGG AGCTGGAGAA GCGGAGACAA GCGCTCTTGG AGCAGCAGCG TAAAGAGCAG
    GAGCGCCTGG CCCAGCTGGA GCGGGCAGAG CAGGAGAGGA AGGAGCGGGA GCGCCAGGAG
    CAGGAGCGCA AAAGACAACT GGAGCTGGAA AAGCAGCTAG AGAAGCAGCG GGAGCTGGAG
    CGGCAGAGGG AGGAGGAGCG AAGGAAGGAA ATCGAGAGGC GGGAGGCTGC GAAACGGGAG
    CTTGAAAGGC AAAGACAGCT TGAATGGGAA CGGAATCGAA GACAAGAACT ATTAAATCAA
    AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT
    GAATTAGAAG CTCTAAATGA TAAAAAACAT CAATTAGAAG GAAAACTTCA GGATATCAGA
    TGTCGACTGA CAACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA
    ATTGCAGAAA TCACCCACCT ACAACAGCAA TTACAGGAGT CCCAGCAAAT GCTTGGAAGA
    CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTGAAAC AAGTTCAGCA AAACAGTTTG
    CACAGGGATT CACTTCTCAC ACTCAAAAGA GCCTTAGAAG CGAAAGAACT GGCTCGGCAG
    CAGCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GGTCAAAATT ACAGGAGATT
    GATATTTTCA ACAATCAGCT GAAGGAACTT AGAGAAATAC ATAATAAGCA GCAGCTCCAG
    AAACAGAAGT CCATAGAGGC TGAACGACTG AAACAGAAGG AACAAGAACG AAAGATCCTG
    GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CCAGCAGTGG
    CTGGAGCGCG TGCATCAGGA GGATGAGCAG CAGCGGCCAC GAAAACCCCA CGAGGAGGAG
    AAGCTCAAAA GGGAGGAGAG CGTCAAAAAG AAGGACAGTG AGGAGAAGGG CAAACCGGAA
    ATGCAAGACA AGCTGAGTCG GCTTTTCCAC CAGCATCAAG AACCAGCTAA GCCCGCTGTC
    CAGGCACCCT GGTCCACTGC AGAAAAAGGC CCACTTACGA TTTCTGCACA GGAGAATATA
    AAAGTGGTGT ATTACCGAGC ACTTTATCCC TTTGAGTCCA GGAGTCACGA TGAAATCACA
    ATCCAGCCGG GAGATATAGT CATGGTGGAC GAAAGCCAAA CTGGAGAACC GGGATGGCTT
    GGAGGAGAAT TAAAAGGAAA GACGGGATGG TTCCCTGCAA ACTACGCAGA GAAAATCCCA
    GAAAATGAGG TTCCCGCCCC AGTCAAACCA GTGACTGAGG CGGCCTCCAC CCCTACACCC
    AAAGTGGCTG TGTGTGAGAC CCCCGCCCCT TCATCCACAA CCCCTGCAGA GCCCTCCGAG
    ACCCCCAACA ACTGGGCCGA CTTCAGCTCC ACGTGGCCCA CCAGCACGAA TGAGAAACCA
    GAAACCGACA GCTGGGACGC ATGGGCAACC CAGCCCTCCC TCACCGTGCC AAGCGCCGGG
    CAGCTGAGGC AGAGGTCGGC CTTCACTCCA GCCACGGCCA CGGGCTCATC TCCGTCTCCT
    GTTCTAGGCC AGGGTGAAAA GGTGGAGGGG CTACAGGCAC AAGCCCTGTA TCCGTGGAGA
    GCCAAGAAAG ACAACCATTT AAATTTTAAC AAAAACGACG TCATCACCGT ACTGGAGCAG
    CAAGACATGT GGTGGTTTGG AGAAGTTCAA GGCCAGAAGG GTTGGTTCCC CAAGTCCTAC
    GTGAAACTCA TTTCAGGGCC CATAAGGAAA TCGACAAGCA TGGATTCTGG TCCCTCAGAA
    AGTCCTGCAA GTCTAAAGAG GGTAGCTTCC CCAGCAGCCA AGCCAGCCGT GTCGGGAGAA
    GAATTTATTG CCATGTACAC TTACGAGAGT TCTGAGCAAG GCGATTTAAC CTTTCAGCAA
    GGGGATGTGA TTTTGGTTAC CAAGAAAGAT GGTGACTGGT GGACAGGAAC AGTGGGCGAC
    AAGTCCGGCG TCTTCCCTTC TAACTATGTG AGGCTTAAAG ATTCGGAGGG CTCTGGAACT
    GCTGGGAAAA CAGGGAGTTT AGGAAAAAAA CCTGAAATCG CGCAGGTCAT TGCCTCCTAC
    ACAGCTACGG GTCCTGAACA GCTTACCCTG GCCCCCGGTC AGCTGATCCT GATCCGGAAA
    AAGAACCCAG GTGGATGGTG GGAAGGAGAG CTACAGGCAC GTGGGAAAAA GCGCCAGATA
    GGCTGGTTTC CTGCTAATTA CGTCAAACTT TTAAGCCCTG GGACAAGCAA AATCACTCCA
    ACAGACCCAC CGAAGCCAAC AGCTTTCCCA ACAGTGTGCC AGGTGATCGG GATGTACGAC
    TACACCGCGC AGAACGACGA CGAGCTGGCC TTCAGCAAGG GCCAGGTCAT CAATGTCCTC
    AACAAGGAGG ACCCCGACTG GTGGAAGGGT GAAGTCCACG GGCAGGTGGG GCTCTTCCCG
    TCCAACTACG TGAAGTTGAC CACGGACACG GACCCCAGCC AGCAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa02401
    Clone ID Related Accession (Same CDS sequence) XM_024993592.1 , XM_024993593.1
    Accession Version XM_024993592.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5151bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product intersectin-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGCTCAGT TTCCCACACC CTTTGGTGGC AGCCTGGATA TTTGGGCCAT AACTGTAGAG 
    GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT
    GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAGCCTGT TTTAGCACAG
    ATATGGGCGC TCGCTGATAT GAATAATGAT GGAAGGATGG ATCAAGTGGA GTTTTCCATT
    GCTATGAAAC TCATCAAGCT GAAGCTCCAA GGATATCAGC TCCCCTCTGC ACTTCCCCCT
    GTCATGAAAC AGCAACCAGT TGCTATTTCT AGTGCACCAG CCTTTGGTAT GGGAGGTATT
    GCCAGCATGC CGCCGCTGAC CGCTGTTGCT CCTGTACCAA TGGGCTCCAT TCCCGTTGTT
    GGGATGTCTC CCCCACTAGT GTCATCTGTT CCTGCAGCAG GGGTGCCCCC CCTGGCCAAT
    GGGGCGCCCC CGGTCATACA GCCTCTGCCT GCATTTGCTC ATCCTGCTGC CACATTGCCA
    AAGAGTTCTT CCTTTAGTAG ATCTGGCCCA GGGTCGCAGT TAAACACAAA ATTACAGAAG
    GCACAATCAT TTGATGTGGC CAGTGTCCCT CCTACAGCTG AGTGGGCTGT TCCTCAGTCA
    TCCAGGCTGA AATACAGACA GCTCTTCAAT AGTCATGACA AAACCATGAG TGGACACCTA
    ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAATCAAGTT TACCACAGGC TCAGCTGGCT
    TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC
    CTGGCTATGC ACCTCATTGA TGTAGCTATG TCTGGTCAAC CACTGCCACC GGTCCTGCCC
    CCAGAATACA TTCCACCTTC CTTTAGAAGA GTTCGATCTG GTAGTGGTAT ATCTGTCATA
    ACCTCAACTT CGGTGGATCA GAGGTTGCCA GAGGAACCAG TTTTGGAAGA TGAACAGCAA
    CAACTAGAAA AGAAACTACC TGTAACCTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGG
    GGCAACCTGG AGCTGGAGAA GCGGAGACAA GCGCTCTTGG AGCAGCAGCG TAAAGAGCAG
    GAGCGCCTGG CCCAGCTGGA GCGGGCAGAG CAGGAGAGGA AGGAGCGGGA GCGCCAGGAG
    CAGGAGCGCA AAAGACAACT GGAGCTGGAA AAGCAGCTAG AGAAGCAGCG GGAGCTGGAG
    CGGCAGAGGG AGGAGGAGCG AAGGAAGGAA ATCGAGAGGC GGGAGGCTGC GAAACGGGAG
    CTTGAAAGGC AAAGACAGCT TGAATGGGAA CGGAATCGAA GACAAGAACT ATTAAATCAA
    AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT
    GAATTAGAAG CTCTAAATGA TAAAAAACAT CAATTAGAAG GAAAACTTCA GGATATCAGA
    TGTCGACTGA CAACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA
    ATTGCAGAAA TCACCCACCT ACAACAGCAA TTACAGGAGT CCCAGCAAAT GCTTGGAAGA
    CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTGAAAC AAGTTCAGCA AAACAGTTTG
    CACAGGGATT CACTTCTCAC ACTCAAAAGA GCCTTAGAAG CGAAAGAACT GGCTCGGCAG
    CAGCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GGTCAAAATT ACAGGAGATT
    GATATTTTCA ACAATCAGCT GAAGGAACTT AGAGAAATAC ATAATAAGCA GCAGCTCCAG
    AAACAGAAGT CCATAGAGGC TGAACGACTG AAACAGAAGG AACAAGAACG AAAGATCCTG
    GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CCAGCAGTGG
    CTGGAGCGCG TGCATCAGGA GGATGAGCAG CAGCGGCCAC GAAAACCCCA CGAGGAGGAG
    AAGCTCAAAA GGGAGGAGAG CGTCAAAAAG AAGGACAGTG AGGAGAAGGG CAAACCGGAA
    ATGCAAGACA AGCTGAGTCG GCTTTTCCAC CAGCATCAAG AACCAGCTAA GCCCGCTGTC
    CAGGCACCCT GGTCCACTGC AGAAAAAGGC CCACTTACGA TTTCTGCACA GGAGAATATA
    AAAGTGGTGT ATTACCGAGC ACTTTATCCC TTTGAGTCCA GGAGTCACGA TGAAATCACA
    ATCCAGCCGG GAGATATAGT CATGGTGGAC GAAAGCCAAA CTGGAGAACC GGGATGGCTT
    GGAGGAGAAT TAAAAGGAAA GACGGGATGG TTCCCTGCAA ACTACGCAGA GAAAATCCCA
    GAAAATGAGG TTCCCGCCCC AGTCAAACCA GTGACTGAGG CGGCCTCCAC CCCTACACCC
    AAAGTGGCTG TGTGTGAGAC CCCCGCCCCT TCATCCACAA CCCCTGCAGA GCCCTCCGAG
    ACCCCCAACA ACTGGGCCGA CTTCAGCTCC ACGTGGCCCA CCAGCACGAA TGAGAAACCA
    GAAACCGACA GCTGGGACGC ATGGGCAACC CAGCCCTCCC TCACCGTGCC AAGCGCCGGG
    CAGCTGAGGC AGAGGTCGGC CTTCACTCCA GCCACGGCCA CGGGCTCATC TCCGTCTCCT
    GTTCTAGGCC AGGGTGAAAA GGTGGAGGGG CTACAGGCAC AAGCCCTGTA TCCGTGGAGA
    GCCAAGAAAG ACAACCATTT AAATTTTAAC AAAAACGACG TCATCACCGT ACTGGAGCAG
    CAAGACATGT GGTGGTTTGG AGAAGTTCAA GGCCAGAAGG GTTGGTTCCC CAAGTCCTAC
    GTGAAACTCA TTTCAGGGCC CATAAGGAAA TCGACAAGCA TGGATTCTGG TCCCTCAGAA
    AGTCCTGCAA GTCTAAAGAG GGTAGCTTCC CCAGCAGCCA AGCCAGCCGT GTCGGGAGAA
    GAATTTATTG CCATGTACAC TTACGAGAGT TCTGAGCAAG GCGATTTAAC CTTTCAGCAA
    GGGGATGTGA TTTTGGTTAC CAAGAAAGAT GGTGACTGGT GGACAGGAAC AGTGGGCGAC
    AAGTCCGGCG TCTTCCCTTC TAACTATGTG AGGCTTAAAG ATTCGGAGGG CTCTGGAACT
    GCTGGGAAAA CAGGGAGTTT AGGAAAAAAA CCTGAAATCG CGCAGGTCAT TGCCTCCTAC
    ACAGCTACGG GTCCTGAACA GCTTACCCTG GCCCCCGGTC AGCTGATCCT GATCCGGAAA
    AAGAACCCAG GTGGATGGTG GGAAGGAGAG CTACAGGCAC GTGGGAAAAA GCGCCAGATA
    GGCTGGTTTC CTGCTAATTA CGTCAAACTT TTAAGCCCTG GGACAAGCAA AATCACTCCA
    ACAGACCCAC CGAAGCCAAC AGCTTTCCCA ACAGTGTGCC AGGTGATCGG GATGTACGAC
    TACACCGCGC AGAACGACGA CGAGCTGGCC TTCAGCAAGG GCCAGGTCAT CAATGTCCTC
    AACAAGGAGG ACCCCGACTG GTGGAAGGGT GAAGTCCACG GGCAGGTGGG GCTCTTCCCG
    TCCAACTACG TGAAGTTGAC CACGGACACG GACCCCAGCC AGCAATGGTG CTCAGACTTA
    CACCTCTTGG ATATGCTGAC CCCAACTGAA AGAAAGCGAC AGGGGTACAT CCACGAGCTC
    ATCGTCACGG AGGAGAACTA CGTGAATGAC CTGCAGCTGG TCACAGAGAT CTTTCAGAAA
    CCCCTGATGG AGTCTGAGCT GCTGACAGAA AAAGAGGGTG CTATGATTTT TGTTAACTGG
    AAGGAGCTGA TTATGTGTAA TATCAAACTA CTGAAAGCGC TGAGGGTCCG CAAGAAGATG
    TCTGGGGAGA AGATGCCCGT GAAGATGATC GGAGACATCC TGACGGCCCA GCTGCCACAC
    ATGCAGCCCT ACATCCGCTT CTGCAGCTGC CAGCTCAACG GGGCCGCCTT GATCCAGCAG
    AAGACTGACG AGGCCCCAGA CTTCAAGGAG TTTGTCAAAA GACTGGCCAT GGACCCACGC
    TGTAAGGGAA TGCCCCTGTC TAGTTTTATA CTGAAGCCTA TGCAACGAGT AACGAGATAC
    CCACTGATCA TTAAAAATAT CCTGGAAAAC ACTCCTGAAA ACCACCCAGA CCACAGCCAC
    CTGAAGCACG CCCTGGAGAA GGCGGAGGAG CTGTGCTCCC AGGTGAATGA AGGGGTGCGG
    GAGAAGGAGA ACTCGGACCG ACTGGAGTGG ATCCAGGCCC ACGTGCTGTG TGAAGGCTTG
    TCAGAGCAAC TTGTGTTCAA TTCCGTGACC AACTGCCTGG GGCCGCGCAA GTTCCTGCAC
    AGTGGGAAGC TCTACAAGGC CAAGAGCAAC AAGGAGCTGT ATGGCTTCCT CTTCAACGAC
    TTCCTGCTGC TGACCCAAAT CATCAAGCCC CTGGGCTCCT CGGGCACCGA CAAAGTCTTC
    AGCCCCAAAT CAAACCTGCA GTATAAAATG TACAAAACAC CTATTTTCCT AAATGAGGTT
    CTAGTGAAGT TGCCCACTGA CCCTTCTGGG GATGAGCCCA TCTTCCACAT TTCCCACATT
    GACCGAGTCT ACACCCTCCG AGCAGAGAGC ATAAATGAAA GGACTGCCTG GGTGCAGAAA
    ATCAAAGCAG CGTCTGAACT CTACATAGAG ACAGAGAAGA AGAAGCGGGA GAAGGCGTAC
    CTGGTCCGCT CCCAAAGAGC CACAGGCATC GGAAGGTTGA TGGTGAACGT GGTTGAAGGC
    ATTGAGCTGA AACCCTGTCG CTCCCATGGA AAGAGTAACC CGTACTGTGA GGTGACCATG
    GGCTCCCAGT GTCACATCAC CAAGACGATC CAGGACACTC TGAATCCCAA GTGGAATTCC
    AACTGCCAGT TCTTCATCAG AGACCTGGAG CAGGAGGTGC TGTGCATCAC TGTGTTTGAG
    AGGGACCAGT TCTCGCCAGA TGATTTTTTG GGTCGGACGG AGATCCGCGT GGCCGACATC
    AAGAAGGACC AGGGCTCCAA GGGTCCCGTT ACGAAGTGTC TCCTGCTGCA CGAAGTTCCC
    ACGGGAGAGA TCGTGGTCCG CTTGGACCTG CAGTTGTTCG ATGAGCCGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024849360.1
    CDS278..5428
    Translation

    Target ORF information:

    RefSeq Version XM_024993592.1
    Organism Bos taurus(cattle)
    Definition Bos taurus intersectin 1 (ITSN1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024993592.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGCTCAGT TTCCCACACC CTTTGGTGGC AGCCTGGATA TTTGGGCCAT AACTGTAGAG 
    GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT
    GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAGCCTGT TTTAGCACAG
    ATATGGGCGC TCGCTGATAT GAATAATGAT GGAAGGATGG ATCAAGTGGA GTTTTCCATT
    GCTATGAAAC TCATCAAGCT GAAGCTCCAA GGATATCAGC TCCCCTCTGC ACTTCCCCCT
    GTCATGAAAC AGCAACCAGT TGCTATTTCT AGTGCACCAG CCTTTGGTAT GGGAGGTATT
    GCCAGCATGC CGCCGCTGAC CGCTGTTGCT CCTGTACCAA TGGGCTCCAT TCCCGTTGTT
    GGGATGTCTC CCCCACTAGT GTCATCTGTT CCTGCAGCAG GGGTGCCCCC CCTGGCCAAT
    GGGGCGCCCC CGGTCATACA GCCTCTGCCT GCATTTGCTC ATCCTGCTGC CACATTGCCA
    AAGAGTTCTT CCTTTAGTAG ATCTGGCCCA GGGTCGCAGT TAAACACAAA ATTACAGAAG
    GCACAATCAT TTGATGTGGC CAGTGTCCCT CCTACAGCTG AGTGGGCTGT TCCTCAGTCA
    TCCAGGCTGA AATACAGACA GCTCTTCAAT AGTCATGACA AAACCATGAG TGGACACCTA
    ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAATCAAGTT TACCACAGGC TCAGCTGGCT
    TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC
    CTGGCTATGC ACCTCATTGA TGTAGCTATG TCTGGTCAAC CACTGCCACC GGTCCTGCCC
    CCAGAATACA TTCCACCTTC CTTTAGAAGA GTTCGATCTG GTAGTGGTAT ATCTGTCATA
    ACCTCAACTT CGGTGGATCA GAGGTTGCCA GAGGAACCAG TTTTGGAAGA TGAACAGCAA
    CAACTAGAAA AGAAACTACC TGTAACCTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGG
    GGCAACCTGG AGCTGGAGAA GCGGAGACAA GCGCTCTTGG AGCAGCAGCG TAAAGAGCAG
    GAGCGCCTGG CCCAGCTGGA GCGGGCAGAG CAGGAGAGGA AGGAGCGGGA GCGCCAGGAG
    CAGGAGCGCA AAAGACAACT GGAGCTGGAA AAGCAGCTAG AGAAGCAGCG GGAGCTGGAG
    CGGCAGAGGG AGGAGGAGCG AAGGAAGGAA ATCGAGAGGC GGGAGGCTGC GAAACGGGAG
    CTTGAAAGGC AAAGACAGCT TGAATGGGAA CGGAATCGAA GACAAGAACT ATTAAATCAA
    AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT
    GAATTAGAAG CTCTAAATGA TAAAAAACAT CAATTAGAAG GAAAACTTCA GGATATCAGA
    TGTCGACTGA CAACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA
    ATTGCAGAAA TCACCCACCT ACAACAGCAA TTACAGGAGT CCCAGCAAAT GCTTGGAAGA
    CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTGAAAC AAGTTCAGCA AAACAGTTTG
    CACAGGGATT CACTTCTCAC ACTCAAAAGA GCCTTAGAAG CGAAAGAACT GGCTCGGCAG
    CAGCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GGTCAAAATT ACAGGAGATT
    GATATTTTCA ACAATCAGCT GAAGGAACTT AGAGAAATAC ATAATAAGCA GCAGCTCCAG
    AAACAGAAGT CCATAGAGGC TGAACGACTG AAACAGAAGG AACAAGAACG AAAGATCCTG
    GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CCAGCAGTGG
    CTGGAGCGCG TGCATCAGGA GGATGAGCAG CAGCGGCCAC GAAAACCCCA CGAGGAGGAG
    AAGCTCAAAA GGGAGGAGAG CGTCAAAAAG AAGGACAGTG AGGAGAAGGG CAAACCGGAA
    ATGCAAGACA AGCTGAGTCG GCTTTTCCAC CAGCATCAAG AACCAGCTAA GCCCGCTGTC
    CAGGCACCCT GGTCCACTGC AGAAAAAGGC CCACTTACGA TTTCTGCACA GGAGAATATA
    AAAGTGGTGT ATTACCGAGC ACTTTATCCC TTTGAGTCCA GGAGTCACGA TGAAATCACA
    ATCCAGCCGG GAGATATAGT CATGGTGGAC GAAAGCCAAA CTGGAGAACC GGGATGGCTT
    GGAGGAGAAT TAAAAGGAAA GACGGGATGG TTCCCTGCAA ACTACGCAGA GAAAATCCCA
    GAAAATGAGG TTCCCGCCCC AGTCAAACCA GTGACTGAGG CGGCCTCCAC CCCTACACCC
    AAAGTGGCTG TGTGTGAGAC CCCCGCCCCT TCATCCACAA CCCCTGCAGA GCCCTCCGAG
    ACCCCCAACA ACTGGGCCGA CTTCAGCTCC ACGTGGCCCA CCAGCACGAA TGAGAAACCA
    GAAACCGACA GCTGGGACGC ATGGGCAACC CAGCCCTCCC TCACCGTGCC AAGCGCCGGG
    CAGCTGAGGC AGAGGTCGGC CTTCACTCCA GCCACGGCCA CGGGCTCATC TCCGTCTCCT
    GTTCTAGGCC AGGGTGAAAA GGTGGAGGGG CTACAGGCAC AAGCCCTGTA TCCGTGGAGA
    GCCAAGAAAG ACAACCATTT AAATTTTAAC AAAAACGACG TCATCACCGT ACTGGAGCAG
    CAAGACATGT GGTGGTTTGG AGAAGTTCAA GGCCAGAAGG GTTGGTTCCC CAAGTCCTAC
    GTGAAACTCA TTTCAGGGCC CATAAGGAAA TCGACAAGCA TGGATTCTGG TCCCTCAGAA
    AGTCCTGCAA GTCTAAAGAG GGTAGCTTCC CCAGCAGCCA AGCCAGCCGT GTCGGGAGAA
    GAATTTATTG CCATGTACAC TTACGAGAGT TCTGAGCAAG GCGATTTAAC CTTTCAGCAA
    GGGGATGTGA TTTTGGTTAC CAAGAAAGAT GGTGACTGGT GGACAGGAAC AGTGGGCGAC
    AAGTCCGGCG TCTTCCCTTC TAACTATGTG AGGCTTAAAG ATTCGGAGGG CTCTGGAACT
    GCTGGGAAAA CAGGGAGTTT AGGAAAAAAA CCTGAAATCG CGCAGGTCAT TGCCTCCTAC
    ACAGCTACGG GTCCTGAACA GCTTACCCTG GCCCCCGGTC AGCTGATCCT GATCCGGAAA
    AAGAACCCAG GTGGATGGTG GGAAGGAGAG CTACAGGCAC GTGGGAAAAA GCGCCAGATA
    GGCTGGTTTC CTGCTAATTA CGTCAAACTT TTAAGCCCTG GGACAAGCAA AATCACTCCA
    ACAGACCCAC CGAAGCCAAC AGCTTTCCCA ACAGTGTGCC AGGTGATCGG GATGTACGAC
    TACACCGCGC AGAACGACGA CGAGCTGGCC TTCAGCAAGG GCCAGGTCAT CAATGTCCTC
    AACAAGGAGG ACCCCGACTG GTGGAAGGGT GAAGTCCACG GGCAGGTGGG GCTCTTCCCG
    TCCAACTACG TGAAGTTGAC CACGGACACG GACCCCAGCC AGCAATGGTG CTCAGACTTA
    CACCTCTTGG ATATGCTGAC CCCAACTGAA AGAAAGCGAC AGGGGTACAT CCACGAGCTC
    ATCGTCACGG AGGAGAACTA CGTGAATGAC CTGCAGCTGG TCACAGAGAT CTTTCAGAAA
    CCCCTGATGG AGTCTGAGCT GCTGACAGAA AAAGAGGGTG CTATGATTTT TGTTAACTGG
    AAGGAGCTGA TTATGTGTAA TATCAAACTA CTGAAAGCGC TGAGGGTCCG CAAGAAGATG
    TCTGGGGAGA AGATGCCCGT GAAGATGATC GGAGACATCC TGACGGCCCA GCTGCCACAC
    ATGCAGCCCT ACATCCGCTT CTGCAGCTGC CAGCTCAACG GGGCCGCCTT GATCCAGCAG
    AAGACTGACG AGGCCCCAGA CTTCAAGGAG TTTGTCAAAA GACTGGCCAT GGACCCACGC
    TGTAAGGGAA TGCCCCTGTC TAGTTTTATA CTGAAGCCTA TGCAACGAGT AACGAGATAC
    CCACTGATCA TTAAAAATAT CCTGGAAAAC ACTCCTGAAA ACCACCCAGA CCACAGCCAC
    CTGAAGCACG CCCTGGAGAA GGCGGAGGAG CTGTGCTCCC AGGTGAATGA AGGGGTGCGG
    GAGAAGGAGA ACTCGGACCG ACTGGAGTGG ATCCAGGCCC ACGTGCTGTG TGAAGGCTTG
    TCAGAGCAAC TTGTGTTCAA TTCCGTGACC AACTGCCTGG GGCCGCGCAA GTTCCTGCAC
    AGTGGGAAGC TCTACAAGGC CAAGAGCAAC AAGGAGCTGT ATGGCTTCCT CTTCAACGAC
    TTCCTGCTGC TGACCCAAAT CATCAAGCCC CTGGGCTCCT CGGGCACCGA CAAAGTCTTC
    AGCCCCAAAT CAAACCTGCA GTATAAAATG TACAAAACAC CTATTTTCCT AAATGAGGTT
    CTAGTGAAGT TGCCCACTGA CCCTTCTGGG GATGAGCCCA TCTTCCACAT TTCCCACATT
    GACCGAGTCT ACACCCTCCG AGCAGAGAGC ATAAATGAAA GGACTGCCTG GGTGCAGAAA
    ATCAAAGCAG CGTCTGAACT CTACATAGAG ACAGAGAAGA AGAAGCGGGA GAAGGCGTAC
    CTGGTCCGCT CCCAAAGAGC CACAGGCATC GGAAGGTTGA TGGTGAACGT GGTTGAAGGC
    ATTGAGCTGA AACCCTGTCG CTCCCATGGA AAGAGTAACC CGTACTGTGA GGTGACCATG
    GGCTCCCAGT GTCACATCAC CAAGACGATC CAGGACACTC TGAATCCCAA GTGGAATTCC
    AACTGCCAGT TCTTCATCAG AGACCTGGAG CAGGAGGTGC TGTGCATCAC TGTGTTTGAG
    AGGGACCAGT TCTCGCCAGA TGATTTTTTG GGTCGGACGG AGATCCGCGT GGCCGACATC
    AAGAAGGACC AGGGCTCCAA GGGTCCCGTT ACGAAGTGTC TCCTGCTGCA CGAAGTTCCC
    ACGGGAGAGA TCGTGGTCCG CTTGGACCTG CAGTTGTTCG ATGAGCCGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa02401
    Clone ID Related Accession (Same CDS sequence) XM_024993592.1 , XM_024993593.1
    Accession Version XM_024993593.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5151bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product intersectin-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGCTCAGT TTCCCACACC CTTTGGTGGC AGCCTGGATA TTTGGGCCAT AACTGTAGAG 
    GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT
    GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAGCCTGT TTTAGCACAG
    ATATGGGCGC TCGCTGATAT GAATAATGAT GGAAGGATGG ATCAAGTGGA GTTTTCCATT
    GCTATGAAAC TCATCAAGCT GAAGCTCCAA GGATATCAGC TCCCCTCTGC ACTTCCCCCT
    GTCATGAAAC AGCAACCAGT TGCTATTTCT AGTGCACCAG CCTTTGGTAT GGGAGGTATT
    GCCAGCATGC CGCCGCTGAC CGCTGTTGCT CCTGTACCAA TGGGCTCCAT TCCCGTTGTT
    GGGATGTCTC CCCCACTAGT GTCATCTGTT CCTGCAGCAG GGGTGCCCCC CCTGGCCAAT
    GGGGCGCCCC CGGTCATACA GCCTCTGCCT GCATTTGCTC ATCCTGCTGC CACATTGCCA
    AAGAGTTCTT CCTTTAGTAG ATCTGGCCCA GGGTCGCAGT TAAACACAAA ATTACAGAAG
    GCACAATCAT TTGATGTGGC CAGTGTCCCT CCTACAGCTG AGTGGGCTGT TCCTCAGTCA
    TCCAGGCTGA AATACAGACA GCTCTTCAAT AGTCATGACA AAACCATGAG TGGACACCTA
    ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAATCAAGTT TACCACAGGC TCAGCTGGCT
    TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC
    CTGGCTATGC ACCTCATTGA TGTAGCTATG TCTGGTCAAC CACTGCCACC GGTCCTGCCC
    CCAGAATACA TTCCACCTTC CTTTAGAAGA GTTCGATCTG GTAGTGGTAT ATCTGTCATA
    ACCTCAACTT CGGTGGATCA GAGGTTGCCA GAGGAACCAG TTTTGGAAGA TGAACAGCAA
    CAACTAGAAA AGAAACTACC TGTAACCTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGG
    GGCAACCTGG AGCTGGAGAA GCGGAGACAA GCGCTCTTGG AGCAGCAGCG TAAAGAGCAG
    GAGCGCCTGG CCCAGCTGGA GCGGGCAGAG CAGGAGAGGA AGGAGCGGGA GCGCCAGGAG
    CAGGAGCGCA AAAGACAACT GGAGCTGGAA AAGCAGCTAG AGAAGCAGCG GGAGCTGGAG
    CGGCAGAGGG AGGAGGAGCG AAGGAAGGAA ATCGAGAGGC GGGAGGCTGC GAAACGGGAG
    CTTGAAAGGC AAAGACAGCT TGAATGGGAA CGGAATCGAA GACAAGAACT ATTAAATCAA
    AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT
    GAATTAGAAG CTCTAAATGA TAAAAAACAT CAATTAGAAG GAAAACTTCA GGATATCAGA
    TGTCGACTGA CAACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA
    ATTGCAGAAA TCACCCACCT ACAACAGCAA TTACAGGAGT CCCAGCAAAT GCTTGGAAGA
    CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTGAAAC AAGTTCAGCA AAACAGTTTG
    CACAGGGATT CACTTCTCAC ACTCAAAAGA GCCTTAGAAG CGAAAGAACT GGCTCGGCAG
    CAGCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GGTCAAAATT ACAGGAGATT
    GATATTTTCA ACAATCAGCT GAAGGAACTT AGAGAAATAC ATAATAAGCA GCAGCTCCAG
    AAACAGAAGT CCATAGAGGC TGAACGACTG AAACAGAAGG AACAAGAACG AAAGATCCTG
    GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CCAGCAGTGG
    CTGGAGCGCG TGCATCAGGA GGATGAGCAG CAGCGGCCAC GAAAACCCCA CGAGGAGGAG
    AAGCTCAAAA GGGAGGAGAG CGTCAAAAAG AAGGACAGTG AGGAGAAGGG CAAACCGGAA
    ATGCAAGACA AGCTGAGTCG GCTTTTCCAC CAGCATCAAG AACCAGCTAA GCCCGCTGTC
    CAGGCACCCT GGTCCACTGC AGAAAAAGGC CCACTTACGA TTTCTGCACA GGAGAATATA
    AAAGTGGTGT ATTACCGAGC ACTTTATCCC TTTGAGTCCA GGAGTCACGA TGAAATCACA
    ATCCAGCCGG GAGATATAGT CATGGTGGAC GAAAGCCAAA CTGGAGAACC GGGATGGCTT
    GGAGGAGAAT TAAAAGGAAA GACGGGATGG TTCCCTGCAA ACTACGCAGA GAAAATCCCA
    GAAAATGAGG TTCCCGCCCC AGTCAAACCA GTGACTGAGG CGGCCTCCAC CCCTACACCC
    AAAGTGGCTG TGTGTGAGAC CCCCGCCCCT TCATCCACAA CCCCTGCAGA GCCCTCCGAG
    ACCCCCAACA ACTGGGCCGA CTTCAGCTCC ACGTGGCCCA CCAGCACGAA TGAGAAACCA
    GAAACCGACA GCTGGGACGC ATGGGCAACC CAGCCCTCCC TCACCGTGCC AAGCGCCGGG
    CAGCTGAGGC AGAGGTCGGC CTTCACTCCA GCCACGGCCA CGGGCTCATC TCCGTCTCCT
    GTTCTAGGCC AGGGTGAAAA GGTGGAGGGG CTACAGGCAC AAGCCCTGTA TCCGTGGAGA
    GCCAAGAAAG ACAACCATTT AAATTTTAAC AAAAACGACG TCATCACCGT ACTGGAGCAG
    CAAGACATGT GGTGGTTTGG AGAAGTTCAA GGCCAGAAGG GTTGGTTCCC CAAGTCCTAC
    GTGAAACTCA TTTCAGGGCC CATAAGGAAA TCGACAAGCA TGGATTCTGG TCCCTCAGAA
    AGTCCTGCAA GTCTAAAGAG GGTAGCTTCC CCAGCAGCCA AGCCAGCCGT GTCGGGAGAA
    GAATTTATTG CCATGTACAC TTACGAGAGT TCTGAGCAAG GCGATTTAAC CTTTCAGCAA
    GGGGATGTGA TTTTGGTTAC CAAGAAAGAT GGTGACTGGT GGACAGGAAC AGTGGGCGAC
    AAGTCCGGCG TCTTCCCTTC TAACTATGTG AGGCTTAAAG ATTCGGAGGG CTCTGGAACT
    GCTGGGAAAA CAGGGAGTTT AGGAAAAAAA CCTGAAATCG CGCAGGTCAT TGCCTCCTAC
    ACAGCTACGG GTCCTGAACA GCTTACCCTG GCCCCCGGTC AGCTGATCCT GATCCGGAAA
    AAGAACCCAG GTGGATGGTG GGAAGGAGAG CTACAGGCAC GTGGGAAAAA GCGCCAGATA
    GGCTGGTTTC CTGCTAATTA CGTCAAACTT TTAAGCCCTG GGACAAGCAA AATCACTCCA
    ACAGACCCAC CGAAGCCAAC AGCTTTCCCA ACAGTGTGCC AGGTGATCGG GATGTACGAC
    TACACCGCGC AGAACGACGA CGAGCTGGCC TTCAGCAAGG GCCAGGTCAT CAATGTCCTC
    AACAAGGAGG ACCCCGACTG GTGGAAGGGT GAAGTCCACG GGCAGGTGGG GCTCTTCCCG
    TCCAACTACG TGAAGTTGAC CACGGACACG GACCCCAGCC AGCAATGGTG CTCAGACTTA
    CACCTCTTGG ATATGCTGAC CCCAACTGAA AGAAAGCGAC AGGGGTACAT CCACGAGCTC
    ATCGTCACGG AGGAGAACTA CGTGAATGAC CTGCAGCTGG TCACAGAGAT CTTTCAGAAA
    CCCCTGATGG AGTCTGAGCT GCTGACAGAA AAAGAGGGTG CTATGATTTT TGTTAACTGG
    AAGGAGCTGA TTATGTGTAA TATCAAACTA CTGAAAGCGC TGAGGGTCCG CAAGAAGATG
    TCTGGGGAGA AGATGCCCGT GAAGATGATC GGAGACATCC TGACGGCCCA GCTGCCACAC
    ATGCAGCCCT ACATCCGCTT CTGCAGCTGC CAGCTCAACG GGGCCGCCTT GATCCAGCAG
    AAGACTGACG AGGCCCCAGA CTTCAAGGAG TTTGTCAAAA GACTGGCCAT GGACCCACGC
    TGTAAGGGAA TGCCCCTGTC TAGTTTTATA CTGAAGCCTA TGCAACGAGT AACGAGATAC
    CCACTGATCA TTAAAAATAT CCTGGAAAAC ACTCCTGAAA ACCACCCAGA CCACAGCCAC
    CTGAAGCACG CCCTGGAGAA GGCGGAGGAG CTGTGCTCCC AGGTGAATGA AGGGGTGCGG
    GAGAAGGAGA ACTCGGACCG ACTGGAGTGG ATCCAGGCCC ACGTGCTGTG TGAAGGCTTG
    TCAGAGCAAC TTGTGTTCAA TTCCGTGACC AACTGCCTGG GGCCGCGCAA GTTCCTGCAC
    AGTGGGAAGC TCTACAAGGC CAAGAGCAAC AAGGAGCTGT ATGGCTTCCT CTTCAACGAC
    TTCCTGCTGC TGACCCAAAT CATCAAGCCC CTGGGCTCCT CGGGCACCGA CAAAGTCTTC
    AGCCCCAAAT CAAACCTGCA GTATAAAATG TACAAAACAC CTATTTTCCT AAATGAGGTT
    CTAGTGAAGT TGCCCACTGA CCCTTCTGGG GATGAGCCCA TCTTCCACAT TTCCCACATT
    GACCGAGTCT ACACCCTCCG AGCAGAGAGC ATAAATGAAA GGACTGCCTG GGTGCAGAAA
    ATCAAAGCAG CGTCTGAACT CTACATAGAG ACAGAGAAGA AGAAGCGGGA GAAGGCGTAC
    CTGGTCCGCT CCCAAAGAGC CACAGGCATC GGAAGGTTGA TGGTGAACGT GGTTGAAGGC
    ATTGAGCTGA AACCCTGTCG CTCCCATGGA AAGAGTAACC CGTACTGTGA GGTGACCATG
    GGCTCCCAGT GTCACATCAC CAAGACGATC CAGGACACTC TGAATCCCAA GTGGAATTCC
    AACTGCCAGT TCTTCATCAG AGACCTGGAG CAGGAGGTGC TGTGCATCAC TGTGTTTGAG
    AGGGACCAGT TCTCGCCAGA TGATTTTTTG GGTCGGACGG AGATCCGCGT GGCCGACATC
    AAGAAGGACC AGGGCTCCAA GGGTCCCGTT ACGAAGTGTC TCCTGCTGCA CGAAGTTCCC
    ACGGGAGAGA TCGTGGTCCG CTTGGACCTG CAGTTGTTCG ATGAGCCGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024849361.1
    CDS195..5345
    Translation

    Target ORF information:

    RefSeq Version XM_024993593.1
    Organism Bos taurus(cattle)
    Definition Bos taurus intersectin 1 (ITSN1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024993593.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGCTCAGT TTCCCACACC CTTTGGTGGC AGCCTGGATA TTTGGGCCAT AACTGTAGAG 
    GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT
    GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAGCCTGT TTTAGCACAG
    ATATGGGCGC TCGCTGATAT GAATAATGAT GGAAGGATGG ATCAAGTGGA GTTTTCCATT
    GCTATGAAAC TCATCAAGCT GAAGCTCCAA GGATATCAGC TCCCCTCTGC ACTTCCCCCT
    GTCATGAAAC AGCAACCAGT TGCTATTTCT AGTGCACCAG CCTTTGGTAT GGGAGGTATT
    GCCAGCATGC CGCCGCTGAC CGCTGTTGCT CCTGTACCAA TGGGCTCCAT TCCCGTTGTT
    GGGATGTCTC CCCCACTAGT GTCATCTGTT CCTGCAGCAG GGGTGCCCCC CCTGGCCAAT
    GGGGCGCCCC CGGTCATACA GCCTCTGCCT GCATTTGCTC ATCCTGCTGC CACATTGCCA
    AAGAGTTCTT CCTTTAGTAG ATCTGGCCCA GGGTCGCAGT TAAACACAAA ATTACAGAAG
    GCACAATCAT TTGATGTGGC CAGTGTCCCT CCTACAGCTG AGTGGGCTGT TCCTCAGTCA
    TCCAGGCTGA AATACAGACA GCTCTTCAAT AGTCATGACA AAACCATGAG TGGACACCTA
    ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAATCAAGTT TACCACAGGC TCAGCTGGCT
    TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC
    CTGGCTATGC ACCTCATTGA TGTAGCTATG TCTGGTCAAC CACTGCCACC GGTCCTGCCC
    CCAGAATACA TTCCACCTTC CTTTAGAAGA GTTCGATCTG GTAGTGGTAT ATCTGTCATA
    ACCTCAACTT CGGTGGATCA GAGGTTGCCA GAGGAACCAG TTTTGGAAGA TGAACAGCAA
    CAACTAGAAA AGAAACTACC TGTAACCTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGG
    GGCAACCTGG AGCTGGAGAA GCGGAGACAA GCGCTCTTGG AGCAGCAGCG TAAAGAGCAG
    GAGCGCCTGG CCCAGCTGGA GCGGGCAGAG CAGGAGAGGA AGGAGCGGGA GCGCCAGGAG
    CAGGAGCGCA AAAGACAACT GGAGCTGGAA AAGCAGCTAG AGAAGCAGCG GGAGCTGGAG
    CGGCAGAGGG AGGAGGAGCG AAGGAAGGAA ATCGAGAGGC GGGAGGCTGC GAAACGGGAG
    CTTGAAAGGC AAAGACAGCT TGAATGGGAA CGGAATCGAA GACAAGAACT ATTAAATCAA
    AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT
    GAATTAGAAG CTCTAAATGA TAAAAAACAT CAATTAGAAG GAAAACTTCA GGATATCAGA
    TGTCGACTGA CAACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA
    ATTGCAGAAA TCACCCACCT ACAACAGCAA TTACAGGAGT CCCAGCAAAT GCTTGGAAGA
    CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTGAAAC AAGTTCAGCA AAACAGTTTG
    CACAGGGATT CACTTCTCAC ACTCAAAAGA GCCTTAGAAG CGAAAGAACT GGCTCGGCAG
    CAGCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GGTCAAAATT ACAGGAGATT
    GATATTTTCA ACAATCAGCT GAAGGAACTT AGAGAAATAC ATAATAAGCA GCAGCTCCAG
    AAACAGAAGT CCATAGAGGC TGAACGACTG AAACAGAAGG AACAAGAACG AAAGATCCTG
    GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CCAGCAGTGG
    CTGGAGCGCG TGCATCAGGA GGATGAGCAG CAGCGGCCAC GAAAACCCCA CGAGGAGGAG
    AAGCTCAAAA GGGAGGAGAG CGTCAAAAAG AAGGACAGTG AGGAGAAGGG CAAACCGGAA
    ATGCAAGACA AGCTGAGTCG GCTTTTCCAC CAGCATCAAG AACCAGCTAA GCCCGCTGTC
    CAGGCACCCT GGTCCACTGC AGAAAAAGGC CCACTTACGA TTTCTGCACA GGAGAATATA
    AAAGTGGTGT ATTACCGAGC ACTTTATCCC TTTGAGTCCA GGAGTCACGA TGAAATCACA
    ATCCAGCCGG GAGATATAGT CATGGTGGAC GAAAGCCAAA CTGGAGAACC GGGATGGCTT
    GGAGGAGAAT TAAAAGGAAA GACGGGATGG TTCCCTGCAA ACTACGCAGA GAAAATCCCA
    GAAAATGAGG TTCCCGCCCC AGTCAAACCA GTGACTGAGG CGGCCTCCAC CCCTACACCC
    AAAGTGGCTG TGTGTGAGAC CCCCGCCCCT TCATCCACAA CCCCTGCAGA GCCCTCCGAG
    ACCCCCAACA ACTGGGCCGA CTTCAGCTCC ACGTGGCCCA CCAGCACGAA TGAGAAACCA
    GAAACCGACA GCTGGGACGC ATGGGCAACC CAGCCCTCCC TCACCGTGCC AAGCGCCGGG
    CAGCTGAGGC AGAGGTCGGC CTTCACTCCA GCCACGGCCA CGGGCTCATC TCCGTCTCCT
    GTTCTAGGCC AGGGTGAAAA GGTGGAGGGG CTACAGGCAC AAGCCCTGTA TCCGTGGAGA
    GCCAAGAAAG ACAACCATTT AAATTTTAAC AAAAACGACG TCATCACCGT ACTGGAGCAG
    CAAGACATGT GGTGGTTTGG AGAAGTTCAA GGCCAGAAGG GTTGGTTCCC CAAGTCCTAC
    GTGAAACTCA TTTCAGGGCC CATAAGGAAA TCGACAAGCA TGGATTCTGG TCCCTCAGAA
    AGTCCTGCAA GTCTAAAGAG GGTAGCTTCC CCAGCAGCCA AGCCAGCCGT GTCGGGAGAA
    GAATTTATTG CCATGTACAC TTACGAGAGT TCTGAGCAAG GCGATTTAAC CTTTCAGCAA
    GGGGATGTGA TTTTGGTTAC CAAGAAAGAT GGTGACTGGT GGACAGGAAC AGTGGGCGAC
    AAGTCCGGCG TCTTCCCTTC TAACTATGTG AGGCTTAAAG ATTCGGAGGG CTCTGGAACT
    GCTGGGAAAA CAGGGAGTTT AGGAAAAAAA CCTGAAATCG CGCAGGTCAT TGCCTCCTAC
    ACAGCTACGG GTCCTGAACA GCTTACCCTG GCCCCCGGTC AGCTGATCCT GATCCGGAAA
    AAGAACCCAG GTGGATGGTG GGAAGGAGAG CTACAGGCAC GTGGGAAAAA GCGCCAGATA
    GGCTGGTTTC CTGCTAATTA CGTCAAACTT TTAAGCCCTG GGACAAGCAA AATCACTCCA
    ACAGACCCAC CGAAGCCAAC AGCTTTCCCA ACAGTGTGCC AGGTGATCGG GATGTACGAC
    TACACCGCGC AGAACGACGA CGAGCTGGCC TTCAGCAAGG GCCAGGTCAT CAATGTCCTC
    AACAAGGAGG ACCCCGACTG GTGGAAGGGT GAAGTCCACG GGCAGGTGGG GCTCTTCCCG
    TCCAACTACG TGAAGTTGAC CACGGACACG GACCCCAGCC AGCAATGGTG CTCAGACTTA
    CACCTCTTGG ATATGCTGAC CCCAACTGAA AGAAAGCGAC AGGGGTACAT CCACGAGCTC
    ATCGTCACGG AGGAGAACTA CGTGAATGAC CTGCAGCTGG TCACAGAGAT CTTTCAGAAA
    CCCCTGATGG AGTCTGAGCT GCTGACAGAA AAAGAGGGTG CTATGATTTT TGTTAACTGG
    AAGGAGCTGA TTATGTGTAA TATCAAACTA CTGAAAGCGC TGAGGGTCCG CAAGAAGATG
    TCTGGGGAGA AGATGCCCGT GAAGATGATC GGAGACATCC TGACGGCCCA GCTGCCACAC
    ATGCAGCCCT ACATCCGCTT CTGCAGCTGC CAGCTCAACG GGGCCGCCTT GATCCAGCAG
    AAGACTGACG AGGCCCCAGA CTTCAAGGAG TTTGTCAAAA GACTGGCCAT GGACCCACGC
    TGTAAGGGAA TGCCCCTGTC TAGTTTTATA CTGAAGCCTA TGCAACGAGT AACGAGATAC
    CCACTGATCA TTAAAAATAT CCTGGAAAAC ACTCCTGAAA ACCACCCAGA CCACAGCCAC
    CTGAAGCACG CCCTGGAGAA GGCGGAGGAG CTGTGCTCCC AGGTGAATGA AGGGGTGCGG
    GAGAAGGAGA ACTCGGACCG ACTGGAGTGG ATCCAGGCCC ACGTGCTGTG TGAAGGCTTG
    TCAGAGCAAC TTGTGTTCAA TTCCGTGACC AACTGCCTGG GGCCGCGCAA GTTCCTGCAC
    AGTGGGAAGC TCTACAAGGC CAAGAGCAAC AAGGAGCTGT ATGGCTTCCT CTTCAACGAC
    TTCCTGCTGC TGACCCAAAT CATCAAGCCC CTGGGCTCCT CGGGCACCGA CAAAGTCTTC
    AGCCCCAAAT CAAACCTGCA GTATAAAATG TACAAAACAC CTATTTTCCT AAATGAGGTT
    CTAGTGAAGT TGCCCACTGA CCCTTCTGGG GATGAGCCCA TCTTCCACAT TTCCCACATT
    GACCGAGTCT ACACCCTCCG AGCAGAGAGC ATAAATGAAA GGACTGCCTG GGTGCAGAAA
    ATCAAAGCAG CGTCTGAACT CTACATAGAG ACAGAGAAGA AGAAGCGGGA GAAGGCGTAC
    CTGGTCCGCT CCCAAAGAGC CACAGGCATC GGAAGGTTGA TGGTGAACGT GGTTGAAGGC
    ATTGAGCTGA AACCCTGTCG CTCCCATGGA AAGAGTAACC CGTACTGTGA GGTGACCATG
    GGCTCCCAGT GTCACATCAC CAAGACGATC CAGGACACTC TGAATCCCAA GTGGAATTCC
    AACTGCCAGT TCTTCATCAG AGACCTGGAG CAGGAGGTGC TGTGCATCAC TGTGTTTGAG
    AGGGACCAGT TCTCGCCAGA TGATTTTTTG GGTCGGACGG AGATCCGCGT GGCCGACATC
    AAGAAGGACC AGGGCTCCAA GGGTCCCGTT ACGAAGTGTC TCCTGCTGCA CGAAGTTCCC
    ACGGGAGAGA TCGTGGTCCG CTTGGACCTG CAGTTGTTCG ATGAGCCGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa02403
    Clone ID Related Accession (Same CDS sequence) XM_024993603.1
    Accession Version XM_024993603.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4938bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product intersectin-1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCTCAGT TTCCCACACC CTTTGGTGGC AGCCTGGATA TTTGGGCCAT AACTGTAGAG 
    GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT
    GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAGCCTGT TTTAGCACAG
    ATATGGGCGC TCGCTGATAT GAATAATGAT GGAAGGATGG ATCAAGTGGA GTTTTCCATT
    GCTATGAAAC TCATCAAGCT GAAGCTCCAA GGATATCAGC TCCCCTCTGC ACTTCCCCCT
    GTCATGAAAC AGCAACCAGT TGCTATTTCT AGTGCACCAG CCTTTGGTAT GGGAGGTATT
    GCCAGCATGC CGCCGCTGAC CGCTGTTGCT CCTGTACCAA TGGGCTCCAT TCCCGTTGTT
    GGGATGTCTC CCCCACTAGT GTCATCTGTT CCTGCAGCAG GGGTGCCCCC CCTGGCCAAT
    GGGGCGCCCC CGGTCATACA GCCTCTGCCT GCATTTGCTC ATCCTGCTGC CACATTGCCA
    AAGAGTTCTT CCTTTAGTAG ATCTGGCCCA GGGTCGCAGT TAAACACAAA ATTACAGAAG
    GCACAATCAT TTGATGTGGC CAGTGTCCCT CCTACAGCTG AGTGGGCTGT TCCTCAGTCA
    TCCAGGCTGA AATACAGACA GCTCTTCAAT AGTCATGACA AAACCATGAG TGGACACCTA
    ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAATCAAGTT TACCACAGGC TCAGCTGGCT
    TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC
    CTGGCTATGC ACCTCATTGA TGTAGCTATG TCTGGTCAAC CACTGCCACC GGTCCTGCCC
    CCAGAATACA TTCCACCTTC CTTTAGAAGA GTTCGATCTG GTAGTGGTAT ATCTGTCATA
    ACCTCAACTT CGGTGGATCA GAGGTTGCCA GAGGAACCAG TTTTGGAAGA TGAACAGCAA
    CAACTAGAAA AGAAACTACC TGTAACCTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGG
    GGCAACCTGG AGCTGGAGAA GCGGAGACAA GCGCTCTTGG AGCAGCAGCG TAAAGAGCAG
    GAGCGCCTGG CCCAGCTGGA GCGGGCAGAG CAGGAGAGGA AGGAGCGGGA GCGCCAGGAG
    CAGGAGCGCA AAAGACAACT GGAGCTGGAA AAGCAGCTAG AGAAGCAGCG GGAGCTGGAG
    CGGCAGAGGG AGGAGGAGCG AAGGAAGGAA ATCGAGAGGC GGGAGGCTGC GAAACGGGAG
    CTTGAAAGGC AAAGACAGCT TGAATGGGAA CGGAATCGAA GACAAGAACT ATTAAATCAA
    AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT
    GAATTAGAAG CTCTAAATGA TAAAAAACAT CAATTAGAAG GAAAACTTCA GGATATCAGA
    TGTCGACTGA CAACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA
    ATTGCAGAAA TCACCCACCT ACAACAGCAA TTACAGGAGT CCCAGCAAAT GCTTGGAAGA
    CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTGAAAC AAGTTCAGCA AAACAGTTTG
    CACAGGGATT CACTTCTCAC ACTCAAAAGA GCCTTAGAAG CGAAAGAACT GGCTCGGCAG
    CAGCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GGTCAAAATT ACAGGAGATT
    GATATTTTCA ACAATCAGCT GAAGGAACTT AGAGAAATAC ATAATAAGCA GCAGCTCCAG
    AAACAGAAGT CCATAGAGGC TGAACGACTG AAACAGAAGG AACAAGAACG AAAGATCCTG
    GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CCAGCAGTGG
    CTGGAGCGCG TGCATCAGGA GGATGAGCAG CAGCGGCCAC GAAAACCCCA CGAGGAGGAG
    AAGCTCAAAA GGGAGGAGAG CGTCAAAAAG AAGGACAGTG AGGAGAAGGG CAAACCGGAA
    ATGCAAGACA AGCTGAGTCG GCTTTTCCAC CAGCATCAAG AACCAGCTAA GCCCGCTGTC
    CAGGCACCCT GGTCCACTGC AGAAAAAGGC CCACTTACGA TTTCTGCACA GGAGAATATA
    AAAGTGGTGT ATTACCGAGC ACTTTATCCC TTTGAGTCCA GGAGTCACGA TGAAATCACA
    ATCCAGCCGG GAGATATAGT CATGGTGGAC GAAAGCCAAA CTGGAGAACC GGGATGGCTT
    GGAGGAGAAT TAAAAGGAAA GACGGGATGG TTCCCTGCAA ACTACGCAGA GAAAATCCCA
    GAAAATGAGG TTCCCGCCCC AGTCAAACCA GTGACTGAGG CGGCCTCCAC CCCTACACCC
    AAAGTGGCTG TGTGTGAGAC CCCCGCCCCT TCATCCACAA CCCCTGCAGA GCCCTCCGAG
    ACCCCCAACA ACTGGGCCGA CTTCAGCTCC ACGTGGCCCA CCAGCACGAA TGAGAAACCA
    GAAACCGACA GCTGGGACGC ATGGGCAACC CAGCCCTCCC TCACCGTGCC AAGCGCCGGG
    CAGCTGAGGC AGAGGTCGGC CTTCACTCCA GCCACGGCCA CGGGCTCATC TCCGTCTCCT
    GTTCTAGGCC AGGGTGAAAA GGTGGAGGGG CTACAGGCAC AAGCCCTGTA TCCGTGGAGA
    GCCAAGAAAG ACAACCATTT AAATTTTAAC AAAAACGACG TCATCACCGT ACTGGAGCAG
    CAAGACATGT GGTGGTTTGG AGAAGTTCAA GGCCAGAAGG GTTGGTTCCC CAAGTCCTAC
    GTGAAACTCA TTTCAGGGCC CATAAGGAAA TCGACAAGCA TGGATTCTGG TCCCTCAGAA
    AGTCCTGCAA GTCTAAAGAG GGTAGCTTCC CCAGCAGCCA AGCCAGCCGT GTCGGGAGAA
    GAAATCGCGC AGGTCATTGC CTCCTACACA GCTACGGGTC CTGAACAGCT TACCCTGGCC
    CCCGGTCAGC TGATCCTGAT CCGGAAAAAG AACCCAGGTG GATGGTGGGA AGGAGAGCTA
    CAGGCACGTG GGAAAAAGCG CCAGATAGGC TGGTTTCCTG CTAATTACGT CAAACTTTTA
    AGCCCTGGGA CAAGCAAAAT CACTCCAACA GACCCACCGA AGCCAACAGC TTTCCCAACA
    GTGTGCCAGG TGATCGGGAT GTACGACTAC ACCGCGCAGA ACGACGACGA GCTGGCCTTC
    AGCAAGGGCC AGGTCATCAA TGTCCTCAAC AAGGAGGACC CCGACTGGTG GAAGGGTGAA
    GTCCACGGGC AGGTGGGGCT CTTCCCGTCC AACTACGTGA AGTTGACCAC GGACACGGAC
    CCCAGCCAGC AATGGTGCTC AGACTTACAC CTCTTGGATA TGCTGACCCC AACTGAAAGA
    AAGCGACAGG GGTACATCCA CGAGCTCATC GTCACGGAGG AGAACTACGT GAATGACCTG
    CAGCTGGTCA CAGAGATCTT TCAGAAACCC CTGATGGAGT CTGAGCTGCT GACAGAAAAA
    GAGGGTGCTA TGATTTTTGT TAACTGGAAG GAGCTGATTA TGTGTAATAT CAAACTACTG
    AAAGCGCTGA GGGTCCGCAA GAAGATGTCT GGGGAGAAGA TGCCCGTGAA GATGATCGGA
    GACATCCTGA CGGCCCAGCT GCCACACATG CAGCCCTACA TCCGCTTCTG CAGCTGCCAG
    CTCAACGGGG CCGCCTTGAT CCAGCAGAAG ACTGACGAGG CCCCAGACTT CAAGGAGTTT
    GTCAAAAGAC TGGCCATGGA CCCACGCTGT AAGGGAATGC CCCTGTCTAG TTTTATACTG
    AAGCCTATGC AACGAGTAAC GAGATACCCA CTGATCATTA AAAATATCCT GGAAAACACT
    CCTGAAAACC ACCCAGACCA CAGCCACCTG AAGCACGCCC TGGAGAAGGC GGAGGAGCTG
    TGCTCCCAGG TGAATGAAGG GGTGCGGGAG AAGGAGAACT CGGACCGACT GGAGTGGATC
    CAGGCCCACG TGCTGTGTGA AGGCTTGTCA GAGCAACTTG TGTTCAATTC CGTGACCAAC
    TGCCTGGGGC CGCGCAAGTT CCTGCACAGT GGGAAGCTCT ACAAGGCCAA GAGCAACAAG
    GAGCTGTATG GCTTCCTCTT CAACGACTTC CTGCTGCTGA CCCAAATCAT CAAGCCCCTG
    GGCTCCTCGG GCACCGACAA AGTCTTCAGC CCCAAATCAA ACCTGCAGTA TAAAATGTAC
    AAAACACCTA TTTTCCTAAA TGAGGTTCTA GTGAAGTTGC CCACTGACCC TTCTGGGGAT
    GAGCCCATCT TCCACATTTC CCACATTGAC CGAGTCTACA CCCTCCGAGC AGAGAGCATA
    AATGAAAGGA CTGCCTGGGT GCAGAAAATC AAAGCAGCGT CTGAACTCTA CATAGAGACA
    GAGAAGAAGA AGCGGGAGAA GGCGTACCTG GTCCGCTCCC AAAGAGCCAC AGGCATCGGA
    AGGTTGATGG TGAACGTGGT TGAAGGCATT GAGCTGAAAC CCTGTCGCTC CCATGGAAAG
    AGTAACCCGT ACTGTGAGGT GACCATGGGC TCCCAGTGTC ACATCACCAA GACGATCCAG
    GACACTCTGA ATCCCAAGTG GAATTCCAAC TGCCAGTTCT TCATCAGAGA CCTGGAGCAG
    GAGGTGCTGT GCATCACTGT GTTTGAGAGG GACCAGTTCT CGCCAGATGA TTTTTTGGGT
    CGGACGGAGA TCCGCGTGGC CGACATCAAG AAGGACCAGG GCTCCAAGGG TCCCGTTACG
    AAGTGTCTCC TGCTGCACGA AGTTCCCACG GGAGAGATCG TGGTCCGCTT GGACCTGCAG
    TTGTTCGATG AGCCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024849371.1
    CDS279..5216
    Translation

    Target ORF information:

    RefSeq Version XM_024993603.1
    Organism Bos taurus(cattle)
    Definition Bos taurus intersectin 1 (ITSN1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024993603.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCTCAGT TTCCCACACC CTTTGGTGGC AGCCTGGATA TTTGGGCCAT AACTGTAGAG 
    GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT
    GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAGCCTGT TTTAGCACAG
    ATATGGGCGC TCGCTGATAT GAATAATGAT GGAAGGATGG ATCAAGTGGA GTTTTCCATT
    GCTATGAAAC TCATCAAGCT GAAGCTCCAA GGATATCAGC TCCCCTCTGC ACTTCCCCCT
    GTCATGAAAC AGCAACCAGT TGCTATTTCT AGTGCACCAG CCTTTGGTAT GGGAGGTATT
    GCCAGCATGC CGCCGCTGAC CGCTGTTGCT CCTGTACCAA TGGGCTCCAT TCCCGTTGTT
    GGGATGTCTC CCCCACTAGT GTCATCTGTT CCTGCAGCAG GGGTGCCCCC CCTGGCCAAT
    GGGGCGCCCC CGGTCATACA GCCTCTGCCT GCATTTGCTC ATCCTGCTGC CACATTGCCA
    AAGAGTTCTT CCTTTAGTAG ATCTGGCCCA GGGTCGCAGT TAAACACAAA ATTACAGAAG
    GCACAATCAT TTGATGTGGC CAGTGTCCCT CCTACAGCTG AGTGGGCTGT TCCTCAGTCA
    TCCAGGCTGA AATACAGACA GCTCTTCAAT AGTCATGACA AAACCATGAG TGGACACCTA
    ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAATCAAGTT TACCACAGGC TCAGCTGGCT
    TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC
    CTGGCTATGC ACCTCATTGA TGTAGCTATG TCTGGTCAAC CACTGCCACC GGTCCTGCCC
    CCAGAATACA TTCCACCTTC CTTTAGAAGA GTTCGATCTG GTAGTGGTAT ATCTGTCATA
    ACCTCAACTT CGGTGGATCA GAGGTTGCCA GAGGAACCAG TTTTGGAAGA TGAACAGCAA
    CAACTAGAAA AGAAACTACC TGTAACCTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGG
    GGCAACCTGG AGCTGGAGAA GCGGAGACAA GCGCTCTTGG AGCAGCAGCG TAAAGAGCAG
    GAGCGCCTGG CCCAGCTGGA GCGGGCAGAG CAGGAGAGGA AGGAGCGGGA GCGCCAGGAG
    CAGGAGCGCA AAAGACAACT GGAGCTGGAA AAGCAGCTAG AGAAGCAGCG GGAGCTGGAG
    CGGCAGAGGG AGGAGGAGCG AAGGAAGGAA ATCGAGAGGC GGGAGGCTGC GAAACGGGAG
    CTTGAAAGGC AAAGACAGCT TGAATGGGAA CGGAATCGAA GACAAGAACT ATTAAATCAA
    AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT
    GAATTAGAAG CTCTAAATGA TAAAAAACAT CAATTAGAAG GAAAACTTCA GGATATCAGA
    TGTCGACTGA CAACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA
    ATTGCAGAAA TCACCCACCT ACAACAGCAA TTACAGGAGT CCCAGCAAAT GCTTGGAAGA
    CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTGAAAC AAGTTCAGCA AAACAGTTTG
    CACAGGGATT CACTTCTCAC ACTCAAAAGA GCCTTAGAAG CGAAAGAACT GGCTCGGCAG
    CAGCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GGTCAAAATT ACAGGAGATT
    GATATTTTCA ACAATCAGCT GAAGGAACTT AGAGAAATAC ATAATAAGCA GCAGCTCCAG
    AAACAGAAGT CCATAGAGGC TGAACGACTG AAACAGAAGG AACAAGAACG AAAGATCCTG
    GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CCAGCAGTGG
    CTGGAGCGCG TGCATCAGGA GGATGAGCAG CAGCGGCCAC GAAAACCCCA CGAGGAGGAG
    AAGCTCAAAA GGGAGGAGAG CGTCAAAAAG AAGGACAGTG AGGAGAAGGG CAAACCGGAA
    ATGCAAGACA AGCTGAGTCG GCTTTTCCAC CAGCATCAAG AACCAGCTAA GCCCGCTGTC
    CAGGCACCCT GGTCCACTGC AGAAAAAGGC CCACTTACGA TTTCTGCACA GGAGAATATA
    AAAGTGGTGT ATTACCGAGC ACTTTATCCC TTTGAGTCCA GGAGTCACGA TGAAATCACA
    ATCCAGCCGG GAGATATAGT CATGGTGGAC GAAAGCCAAA CTGGAGAACC GGGATGGCTT
    GGAGGAGAAT TAAAAGGAAA GACGGGATGG TTCCCTGCAA ACTACGCAGA GAAAATCCCA
    GAAAATGAGG TTCCCGCCCC AGTCAAACCA GTGACTGAGG CGGCCTCCAC CCCTACACCC
    AAAGTGGCTG TGTGTGAGAC CCCCGCCCCT TCATCCACAA CCCCTGCAGA GCCCTCCGAG
    ACCCCCAACA ACTGGGCCGA CTTCAGCTCC ACGTGGCCCA CCAGCACGAA TGAGAAACCA
    GAAACCGACA GCTGGGACGC ATGGGCAACC CAGCCCTCCC TCACCGTGCC AAGCGCCGGG
    CAGCTGAGGC AGAGGTCGGC CTTCACTCCA GCCACGGCCA CGGGCTCATC TCCGTCTCCT
    GTTCTAGGCC AGGGTGAAAA GGTGGAGGGG CTACAGGCAC AAGCCCTGTA TCCGTGGAGA
    GCCAAGAAAG ACAACCATTT AAATTTTAAC AAAAACGACG TCATCACCGT ACTGGAGCAG
    CAAGACATGT GGTGGTTTGG AGAAGTTCAA GGCCAGAAGG GTTGGTTCCC CAAGTCCTAC
    GTGAAACTCA TTTCAGGGCC CATAAGGAAA TCGACAAGCA TGGATTCTGG TCCCTCAGAA
    AGTCCTGCAA GTCTAAAGAG GGTAGCTTCC CCAGCAGCCA AGCCAGCCGT GTCGGGAGAA
    GAAATCGCGC AGGTCATTGC CTCCTACACA GCTACGGGTC CTGAACAGCT TACCCTGGCC
    CCCGGTCAGC TGATCCTGAT CCGGAAAAAG AACCCAGGTG GATGGTGGGA AGGAGAGCTA
    CAGGCACGTG GGAAAAAGCG CCAGATAGGC TGGTTTCCTG CTAATTACGT CAAACTTTTA
    AGCCCTGGGA CAAGCAAAAT CACTCCAACA GACCCACCGA AGCCAACAGC TTTCCCAACA
    GTGTGCCAGG TGATCGGGAT GTACGACTAC ACCGCGCAGA ACGACGACGA GCTGGCCTTC
    AGCAAGGGCC AGGTCATCAA TGTCCTCAAC AAGGAGGACC CCGACTGGTG GAAGGGTGAA
    GTCCACGGGC AGGTGGGGCT CTTCCCGTCC AACTACGTGA AGTTGACCAC GGACACGGAC
    CCCAGCCAGC AATGGTGCTC AGACTTACAC CTCTTGGATA TGCTGACCCC AACTGAAAGA
    AAGCGACAGG GGTACATCCA CGAGCTCATC GTCACGGAGG AGAACTACGT GAATGACCTG
    CAGCTGGTCA CAGAGATCTT TCAGAAACCC CTGATGGAGT CTGAGCTGCT GACAGAAAAA
    GAGGGTGCTA TGATTTTTGT TAACTGGAAG GAGCTGATTA TGTGTAATAT CAAACTACTG
    AAAGCGCTGA GGGTCCGCAA GAAGATGTCT GGGGAGAAGA TGCCCGTGAA GATGATCGGA
    GACATCCTGA CGGCCCAGCT GCCACACATG CAGCCCTACA TCCGCTTCTG CAGCTGCCAG
    CTCAACGGGG CCGCCTTGAT CCAGCAGAAG ACTGACGAGG CCCCAGACTT CAAGGAGTTT
    GTCAAAAGAC TGGCCATGGA CCCACGCTGT AAGGGAATGC CCCTGTCTAG TTTTATACTG
    AAGCCTATGC AACGAGTAAC GAGATACCCA CTGATCATTA AAAATATCCT GGAAAACACT
    CCTGAAAACC ACCCAGACCA CAGCCACCTG AAGCACGCCC TGGAGAAGGC GGAGGAGCTG
    TGCTCCCAGG TGAATGAAGG GGTGCGGGAG AAGGAGAACT CGGACCGACT GGAGTGGATC
    CAGGCCCACG TGCTGTGTGA AGGCTTGTCA GAGCAACTTG TGTTCAATTC CGTGACCAAC
    TGCCTGGGGC CGCGCAAGTT CCTGCACAGT GGGAAGCTCT ACAAGGCCAA GAGCAACAAG
    GAGCTGTATG GCTTCCTCTT CAACGACTTC CTGCTGCTGA CCCAAATCAT CAAGCCCCTG
    GGCTCCTCGG GCACCGACAA AGTCTTCAGC CCCAAATCAA ACCTGCAGTA TAAAATGTAC
    AAAACACCTA TTTTCCTAAA TGAGGTTCTA GTGAAGTTGC CCACTGACCC TTCTGGGGAT
    GAGCCCATCT TCCACATTTC CCACATTGAC CGAGTCTACA CCCTCCGAGC AGAGAGCATA
    AATGAAAGGA CTGCCTGGGT GCAGAAAATC AAAGCAGCGT CTGAACTCTA CATAGAGACA
    GAGAAGAAGA AGCGGGAGAA GGCGTACCTG GTCCGCTCCC AAAGAGCCAC AGGCATCGGA
    AGGTTGATGG TGAACGTGGT TGAAGGCATT GAGCTGAAAC CCTGTCGCTC CCATGGAAAG
    AGTAACCCGT ACTGTGAGGT GACCATGGGC TCCCAGTGTC ACATCACCAA GACGATCCAG
    GACACTCTGA ATCCCAAGTG GAATTCCAAC TGCCAGTTCT TCATCAGAGA CCTGGAGCAG
    GAGGTGCTGT GCATCACTGT GTTTGAGAGG GACCAGTTCT CGCCAGATGA TTTTTTGGGT
    CGGACGGAGA TCCGCGTGGC CGACATCAAG AAGGACCAGG GCTCCAAGGG TCCCGTTACG
    AAGTGTCTCC TGCTGCACGA AGTTCCCACG GGAGAGATCG TGGTCCGCTT GGACCTGCAG
    TTGTTCGATG AGCCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47836
    Clone ID Related Accession (Same CDS sequence) XM_024993595.1
    Accession Version XM_024993595.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4953bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product intersectin-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAATAATG ATGGAAGGAT GGATCAAGTG GAGTTTTCCA TTGCTATGAA ACTCATCAAG 
    CTGAAGCTCC AAGGATATCA GCTCCCCTCT GCACTTCCCC CTGTCATGAA ACAGCAACCA
    GTTGCTATTT CTAGTGCACC AGCCTTTGGT ATGGGAGGTA TTGCCAGCAT GCCGCCGCTG
    ACCGCTGTTG CTCCTGTACC AATGGGCTCC ATTCCCGTTG TTGGGATGTC TCCCCCACTA
    GTGTCATCTG TTCCTGCAGC AGGGGTGCCC CCCCTGGCCA ATGGGGCGCC CCCGGTCATA
    CAGCCTCTGC CTGCATTTGC TCATCCTGCT GCCACATTGC CAAAGAGTTC TTCCTTTAGT
    AGATCTGGCC CAGGGTCGCA GTTAAACACA AAATTACAGA AGGCACAATC ATTTGATGTG
    GCCAGTGTCC CTCCTACAGC TGAGTGGGCT GTTCCTCAGT CATCCAGGCT GAAATACAGA
    CAGCTCTTCA ATAGTCATGA CAAAACCATG AGTGGACACC TAACAGGTCC CCAAGCAAGA
    ACTATTCTTA TGCAATCAAG TTTACCACAG GCTCAGCTGG CTTCAATATG GAATCTTTCT
    GACATTGATC AAGATGGAAA ACTCACAGCA GAAGAATTTA TCCTGGCTAT GCACCTCATT
    GATGTAGCTA TGTCTGGTCA ACCACTGCCA CCGGTCCTGC CCCCAGAATA CATTCCACCT
    TCCTTTAGAA GAGTTCGATC TGGTAGTGGT ATATCTGTCA TAACCTCAAC TTCGGTGGAT
    CAGAGGTTGC CAGAGGAACC AGTTTTGGAA GATGAACAGC AACAACTAGA AAAGAAACTA
    CCTGTAACCT TTGAAGATAA GAAGCGGGAG AACTTTGAAC GGGGCAACCT GGAGCTGGAG
    AAGCGGAGAC AAGCGCTCTT GGAGCAGCAG CGTAAAGAGC AGGAGCGCCT GGCCCAGCTG
    GAGCGGGCAG AGCAGGAGAG GAAGGAGCGG GAGCGCCAGG AGCAGGAGCG CAAAAGACAA
    CTGGAGCTGG AAAAGCAGCT AGAGAAGCAG CGGGAGCTGG AGCGGCAGAG GGAGGAGGAG
    CGAAGGAAGG AAATCGAGAG GCGGGAGGCT GCGAAACGGG AGCTTGAAAG GCAAAGACAG
    CTTGAATGGG AACGGAATCG AAGACAAGAA CTATTAAATC AAAGAAACAA AGAACAAGAA
    GACATAGTTG TACTGAAAGC AAAGAAAAAG ACTTTGGAAT TTGAATTAGA AGCTCTAAAT
    GATAAAAAAC ATCAATTAGA AGGAAAACTT CAGGATATCA GATGTCGACT GACAACCCAA
    AGGCAAGAAA TTGAGAGCAC AAACAAATCT AGAGAGTTGA GAATTGCAGA AATCACCCAC
    CTACAACAGC AATTACAGGA GTCCCAGCAA ATGCTTGGAA GACTTATTCC AGAAAAACAG
    ATACTCAATG ACCAATTGAA ACAAGTTCAG CAAAACAGTT TGCACAGGGA TTCACTTCTC
    ACACTCAAAA GAGCCTTAGA AGCGAAAGAA CTGGCTCGGC AGCAGCTACG AGACCAACTG
    GATGAAGTGG AGAAAGAAAC TAGGTCAAAA TTACAGGAGA TTGATATTTT CAACAATCAG
    CTGAAGGAAC TTAGAGAAAT ACATAATAAG CAGCAGCTCC AGAAACAGAA GTCCATAGAG
    GCTGAACGAC TGAAACAGAA GGAACAAGAA CGAAAGATCC TGGAATTAGA AAAACAAAAA
    GAAGAAGCCC AAAGACGAGC TCAGGAAAGG GACCAGCAGT GGCTGGAGCG CGTGCATCAG
    GAGGATGAGC AGCAGCGGCC ACGAAAACCC CACGAGGAGG AGAAGCTCAA AAGGGAGGAG
    AGCGTCAAAA AGAAGGACAG TGAGGAGAAG GGCAAACCGG AAATGCAAGA CAAGCTGAGT
    CGGCTTTTCC ACCAGCATCA AGAACCAGCT AAGCCCGCTG TCCAGGCACC CTGGTCCACT
    GCAGAAAAAG GCCCACTTAC GATTTCTGCA CAGGAGAATA TAAAAGTGGT GTATTACCGA
    GCACTTTATC CCTTTGAGTC CAGGAGTCAC GATGAAATCA CAATCCAGCC GGGAGATATA
    GTCATGGTGG ACGAAAGCCA AACTGGAGAA CCGGGATGGC TTGGAGGAGA ATTAAAAGGA
    AAGACGGGAT GGTTCCCTGC AAACTACGCA GAGAAAATCC CAGAAAATGA GGTTCCCGCC
    CCAGTCAAAC CAGTGACTGA GGCGGCCTCC ACCCCTACAC CCAAAGTGGC TGTGTGTGAG
    ACCCCCGCCC CTTCATCCAC AACCCCTGCA GAGCCCTCCG AGACCCCCAA CAACTGGGCC
    GACTTCAGCT CCACGTGGCC CACCAGCACG AATGAGAAAC CAGAAACCGA CAGCTGGGAC
    GCATGGGCAA CCCAGCCCTC CCTCACCGTG CCAAGCGCCG GGCAGCTGAG GCAGAGGTCG
    GCCTTCACTC CAGCCACGGC CACGGGCTCA TCTCCGTCTC CTGTTCTAGG CCAGGGTGAA
    AAGGTGGAGG GGCTACAGGC ACAAGCCCTG TATCCGTGGA GAGCCAAGAA AGACAACCAT
    TTAAATTTTA ACAAAAACGA CGTCATCACC GTACTGGAGC AGCAAGACAT GTGGTGGTTT
    GGAGAAGTTC AAGGCCAGAA GGGTTGGTTC CCCAAGTCCT ACGTGAAACT CATTTCAGGG
    CCCATAAGGA AATCGACAAG CATGGATTCT GGTCCCTCAG AAAGTCCTGC AAGTCTAAAG
    AGGGTAGCTT CCCCAGCAGC CAAGCCAGCC GTGTCGGGAG AAGAATTTAT TGCCATGTAC
    ACTTACGAGA GTTCTGAGCA AGGCGATTTA ACCTTTCAGC AAGGGGATGT GATTTTGGTT
    ACCAAGAAAG ATGGTGACTG GTGGACAGGA ACAGTGGGCG ACAAGTCCGG CGTCTTCCCT
    TCTAACTATG TGAGGCTTAA AGATTCGGAG GGCTCTGGAA CTGCTGGGAA AACAGGGAGT
    TTAGGAAAAA AACCTGAAAT CGCGCAGGTC ATTGCCTCCT ACACAGCTAC GGGTCCTGAA
    CAGCTTACCC TGGCCCCCGG TCAGCTGATC CTGATCCGGA AAAAGAACCC AGGTGGATGG
    TGGGAAGGAG AGCTACAGGC ACGTGGGAAA AAGCGCCAGA TAGGCTGGTT TCCTGCTAAT
    TACGTCAAAC TTTTAAGCCC TGGGACAAGC AAAATCACTC CAACAGACCC ACCGAAGCCA
    ACAGCTTTCC CAACAGTGTG CCAGGTGATC GGGATGTACG ACTACACCGC GCAGAACGAC
    GACGAGCTGG CCTTCAGCAA GGGCCAGGTC ATCAATGTCC TCAACAAGGA GGACCCCGAC
    TGGTGGAAGG GTGAAGTCCA CGGGCAGGTG GGGCTCTTCC CGTCCAACTA CGTGAAGTTG
    ACCACGGACA CGGACCCCAG CCAGCAATGG TGCTCAGACT TACACCTCTT GGATATGCTG
    ACCCCAACTG AAAGAAAGCG ACAGGGGTAC ATCCACGAGC TCATCGTCAC GGAGGAGAAC
    TACGTGAATG ACCTGCAGCT GGTCACAGAG ATCTTTCAGA AACCCCTGAT GGAGTCTGAG
    CTGCTGACAG AAAAAGAGGG TGCTATGATT TTTGTTAACT GGAAGGAGCT GATTATGTGT
    AATATCAAAC TACTGAAAGC GCTGAGGGTC CGCAAGAAGA TGTCTGGGGA GAAGATGCCC
    GTGAAGATGA TCGGAGACAT CCTGACGGCC CAGCTGCCAC ACATGCAGCC CTACATCCGC
    TTCTGCAGCT GCCAGCTCAA CGGGGCCGCC TTGATCCAGC AGAAGACTGA CGAGGCCCCA
    GACTTCAAGG AGTTTGTCAA AAGACTGGCC ATGGACCCAC GCTGTAAGGG AATGCCCCTG
    TCTAGTTTTA TACTGAAGCC TATGCAACGA GTAACGAGAT ACCCACTGAT CATTAAAAAT
    ATCCTGGAAA ACACTCCTGA AAACCACCCA GACCACAGCC ACCTGAAGCA CGCCCTGGAG
    AAGGCGGAGG AGCTGTGCTC CCAGGTGAAT GAAGGGGTGC GGGAGAAGGA GAACTCGGAC
    CGACTGGAGT GGATCCAGGC CCACGTGCTG TGTGAAGGCT TGTCAGAGCA ACTTGTGTTC
    AATTCCGTGA CCAACTGCCT GGGGCCGCGC AAGTTCCTGC ACAGTGGGAA GCTCTACAAG
    GCCAAGAGCA ACAAGGAGCT GTATGGCTTC CTCTTCAACG ACTTCCTGCT GCTGACCCAA
    ATCATCAAGC CCCTGGGCTC CTCGGGCACC GACAAAGTCT TCAGCCCCAA ATCAAACCTG
    CAGTATAAAA TGTACAAAAC ACCTATTTTC CTAAATGAGG TTCTAGTGAA GTTGCCCACT
    GACCCTTCTG GGGATGAGCC CATCTTCCAC ATTTCCCACA TTGACCGAGT CTACACCCTC
    CGAGCAGAGA GCATAAATGA AAGGACTGCC TGGGTGCAGA AAATCAAAGC AGCGTCTGAA
    CTCTACATAG AGACAGAGAA GAAGAAGCGG GAGAAGGCGT ACCTGGTCCG CTCCCAAAGA
    GCCACAGGCA TCGGAAGGTT GATGGTGAAC GTGGTTGAAG GCATTGAGCT GAAACCCTGT
    CGCTCCCATG GAAAGAGTAA CCCGTACTGT GAGGTGACCA TGGGCTCCCA GTGTCACATC
    ACCAAGACGA TCCAGGACAC TCTGAATCCC AAGTGGAATT CCAACTGCCA GTTCTTCATC
    AGAGACCTGG AGCAGGAGGT GCTGTGCATC ACTGTGTTTG AGAGGGACCA GTTCTCGCCA
    GATGATTTTT TGGGTCGGAC GGAGATCCGC GTGGCCGACA TCAAGAAGGA CCAGGGCTCC
    AAGGGTCCCG TTACGAAGTG TCTCCTGCTG CACGAAGTTC CCACGGGAGA GATCGTGGTC
    CGCTTGGACC TGCAGTTGTT CGATGAGCCG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024849363.1
    CDS586..5538
    Translation

    Target ORF information:

    RefSeq Version XM_024993595.1
    Organism Bos taurus(cattle)
    Definition Bos taurus intersectin 1 (ITSN1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024993595.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAATAATG ATGGAAGGAT GGATCAAGTG GAGTTTTCCA TTGCTATGAA ACTCATCAAG 
    CTGAAGCTCC AAGGATATCA GCTCCCCTCT GCACTTCCCC CTGTCATGAA ACAGCAACCA
    GTTGCTATTT CTAGTGCACC AGCCTTTGGT ATGGGAGGTA TTGCCAGCAT GCCGCCGCTG
    ACCGCTGTTG CTCCTGTACC AATGGGCTCC ATTCCCGTTG TTGGGATGTC TCCCCCACTA
    GTGTCATCTG TTCCTGCAGC AGGGGTGCCC CCCCTGGCCA ATGGGGCGCC CCCGGTCATA
    CAGCCTCTGC CTGCATTTGC TCATCCTGCT GCCACATTGC CAAAGAGTTC TTCCTTTAGT
    AGATCTGGCC CAGGGTCGCA GTTAAACACA AAATTACAGA AGGCACAATC ATTTGATGTG
    GCCAGTGTCC CTCCTACAGC TGAGTGGGCT GTTCCTCAGT CATCCAGGCT GAAATACAGA
    CAGCTCTTCA ATAGTCATGA CAAAACCATG AGTGGACACC TAACAGGTCC CCAAGCAAGA
    ACTATTCTTA TGCAATCAAG TTTACCACAG GCTCAGCTGG CTTCAATATG GAATCTTTCT
    GACATTGATC AAGATGGAAA ACTCACAGCA GAAGAATTTA TCCTGGCTAT GCACCTCATT
    GATGTAGCTA TGTCTGGTCA ACCACTGCCA CCGGTCCTGC CCCCAGAATA CATTCCACCT
    TCCTTTAGAA GAGTTCGATC TGGTAGTGGT ATATCTGTCA TAACCTCAAC TTCGGTGGAT
    CAGAGGTTGC CAGAGGAACC AGTTTTGGAA GATGAACAGC AACAACTAGA AAAGAAACTA
    CCTGTAACCT TTGAAGATAA GAAGCGGGAG AACTTTGAAC GGGGCAACCT GGAGCTGGAG
    AAGCGGAGAC AAGCGCTCTT GGAGCAGCAG CGTAAAGAGC AGGAGCGCCT GGCCCAGCTG
    GAGCGGGCAG AGCAGGAGAG GAAGGAGCGG GAGCGCCAGG AGCAGGAGCG CAAAAGACAA
    CTGGAGCTGG AAAAGCAGCT AGAGAAGCAG CGGGAGCTGG AGCGGCAGAG GGAGGAGGAG
    CGAAGGAAGG AAATCGAGAG GCGGGAGGCT GCGAAACGGG AGCTTGAAAG GCAAAGACAG
    CTTGAATGGG AACGGAATCG AAGACAAGAA CTATTAAATC AAAGAAACAA AGAACAAGAA
    GACATAGTTG TACTGAAAGC AAAGAAAAAG ACTTTGGAAT TTGAATTAGA AGCTCTAAAT
    GATAAAAAAC ATCAATTAGA AGGAAAACTT CAGGATATCA GATGTCGACT GACAACCCAA
    AGGCAAGAAA TTGAGAGCAC AAACAAATCT AGAGAGTTGA GAATTGCAGA AATCACCCAC
    CTACAACAGC AATTACAGGA GTCCCAGCAA ATGCTTGGAA GACTTATTCC AGAAAAACAG
    ATACTCAATG ACCAATTGAA ACAAGTTCAG CAAAACAGTT TGCACAGGGA TTCACTTCTC
    ACACTCAAAA GAGCCTTAGA AGCGAAAGAA CTGGCTCGGC AGCAGCTACG AGACCAACTG
    GATGAAGTGG AGAAAGAAAC TAGGTCAAAA TTACAGGAGA TTGATATTTT CAACAATCAG
    CTGAAGGAAC TTAGAGAAAT ACATAATAAG CAGCAGCTCC AGAAACAGAA GTCCATAGAG
    GCTGAACGAC TGAAACAGAA GGAACAAGAA CGAAAGATCC TGGAATTAGA AAAACAAAAA
    GAAGAAGCCC AAAGACGAGC TCAGGAAAGG GACCAGCAGT GGCTGGAGCG CGTGCATCAG
    GAGGATGAGC AGCAGCGGCC ACGAAAACCC CACGAGGAGG AGAAGCTCAA AAGGGAGGAG
    AGCGTCAAAA AGAAGGACAG TGAGGAGAAG GGCAAACCGG AAATGCAAGA CAAGCTGAGT
    CGGCTTTTCC ACCAGCATCA AGAACCAGCT AAGCCCGCTG TCCAGGCACC CTGGTCCACT
    GCAGAAAAAG GCCCACTTAC GATTTCTGCA CAGGAGAATA TAAAAGTGGT GTATTACCGA
    GCACTTTATC CCTTTGAGTC CAGGAGTCAC GATGAAATCA CAATCCAGCC GGGAGATATA
    GTCATGGTGG ACGAAAGCCA AACTGGAGAA CCGGGATGGC TTGGAGGAGA ATTAAAAGGA
    AAGACGGGAT GGTTCCCTGC AAACTACGCA GAGAAAATCC CAGAAAATGA GGTTCCCGCC
    CCAGTCAAAC CAGTGACTGA GGCGGCCTCC ACCCCTACAC CCAAAGTGGC TGTGTGTGAG
    ACCCCCGCCC CTTCATCCAC AACCCCTGCA GAGCCCTCCG AGACCCCCAA CAACTGGGCC
    GACTTCAGCT CCACGTGGCC CACCAGCACG AATGAGAAAC CAGAAACCGA CAGCTGGGAC
    GCATGGGCAA CCCAGCCCTC CCTCACCGTG CCAAGCGCCG GGCAGCTGAG GCAGAGGTCG
    GCCTTCACTC CAGCCACGGC CACGGGCTCA TCTCCGTCTC CTGTTCTAGG CCAGGGTGAA
    AAGGTGGAGG GGCTACAGGC ACAAGCCCTG TATCCGTGGA GAGCCAAGAA AGACAACCAT
    TTAAATTTTA ACAAAAACGA CGTCATCACC GTACTGGAGC AGCAAGACAT GTGGTGGTTT
    GGAGAAGTTC AAGGCCAGAA GGGTTGGTTC CCCAAGTCCT ACGTGAAACT CATTTCAGGG
    CCCATAAGGA AATCGACAAG CATGGATTCT GGTCCCTCAG AAAGTCCTGC AAGTCTAAAG
    AGGGTAGCTT CCCCAGCAGC CAAGCCAGCC GTGTCGGGAG AAGAATTTAT TGCCATGTAC
    ACTTACGAGA GTTCTGAGCA AGGCGATTTA ACCTTTCAGC AAGGGGATGT GATTTTGGTT
    ACCAAGAAAG ATGGTGACTG GTGGACAGGA ACAGTGGGCG ACAAGTCCGG CGTCTTCCCT
    TCTAACTATG TGAGGCTTAA AGATTCGGAG GGCTCTGGAA CTGCTGGGAA AACAGGGAGT
    TTAGGAAAAA AACCTGAAAT CGCGCAGGTC ATTGCCTCCT ACACAGCTAC GGGTCCTGAA
    CAGCTTACCC TGGCCCCCGG TCAGCTGATC CTGATCCGGA AAAAGAACCC AGGTGGATGG
    TGGGAAGGAG AGCTACAGGC ACGTGGGAAA AAGCGCCAGA TAGGCTGGTT TCCTGCTAAT
    TACGTCAAAC TTTTAAGCCC TGGGACAAGC AAAATCACTC CAACAGACCC ACCGAAGCCA
    ACAGCTTTCC CAACAGTGTG CCAGGTGATC GGGATGTACG ACTACACCGC GCAGAACGAC
    GACGAGCTGG CCTTCAGCAA GGGCCAGGTC ATCAATGTCC TCAACAAGGA GGACCCCGAC
    TGGTGGAAGG GTGAAGTCCA CGGGCAGGTG GGGCTCTTCC CGTCCAACTA CGTGAAGTTG
    ACCACGGACA CGGACCCCAG CCAGCAATGG TGCTCAGACT TACACCTCTT GGATATGCTG
    ACCCCAACTG AAAGAAAGCG ACAGGGGTAC ATCCACGAGC TCATCGTCAC GGAGGAGAAC
    TACGTGAATG ACCTGCAGCT GGTCACAGAG ATCTTTCAGA AACCCCTGAT GGAGTCTGAG
    CTGCTGACAG AAAAAGAGGG TGCTATGATT TTTGTTAACT GGAAGGAGCT GATTATGTGT
    AATATCAAAC TACTGAAAGC GCTGAGGGTC CGCAAGAAGA TGTCTGGGGA GAAGATGCCC
    GTGAAGATGA TCGGAGACAT CCTGACGGCC CAGCTGCCAC ACATGCAGCC CTACATCCGC
    TTCTGCAGCT GCCAGCTCAA CGGGGCCGCC TTGATCCAGC AGAAGACTGA CGAGGCCCCA
    GACTTCAAGG AGTTTGTCAA AAGACTGGCC ATGGACCCAC GCTGTAAGGG AATGCCCCTG
    TCTAGTTTTA TACTGAAGCC TATGCAACGA GTAACGAGAT ACCCACTGAT CATTAAAAAT
    ATCCTGGAAA ACACTCCTGA AAACCACCCA GACCACAGCC ACCTGAAGCA CGCCCTGGAG
    AAGGCGGAGG AGCTGTGCTC CCAGGTGAAT GAAGGGGTGC GGGAGAAGGA GAACTCGGAC
    CGACTGGAGT GGATCCAGGC CCACGTGCTG TGTGAAGGCT TGTCAGAGCA ACTTGTGTTC
    AATTCCGTGA CCAACTGCCT GGGGCCGCGC AAGTTCCTGC ACAGTGGGAA GCTCTACAAG
    GCCAAGAGCA ACAAGGAGCT GTATGGCTTC CTCTTCAACG ACTTCCTGCT GCTGACCCAA
    ATCATCAAGC CCCTGGGCTC CTCGGGCACC GACAAAGTCT TCAGCCCCAA ATCAAACCTG
    CAGTATAAAA TGTACAAAAC ACCTATTTTC CTAAATGAGG TTCTAGTGAA GTTGCCCACT
    GACCCTTCTG GGGATGAGCC CATCTTCCAC ATTTCCCACA TTGACCGAGT CTACACCCTC
    CGAGCAGAGA GCATAAATGA AAGGACTGCC TGGGTGCAGA AAATCAAAGC AGCGTCTGAA
    CTCTACATAG AGACAGAGAA GAAGAAGCGG GAGAAGGCGT ACCTGGTCCG CTCCCAAAGA
    GCCACAGGCA TCGGAAGGTT GATGGTGAAC GTGGTTGAAG GCATTGAGCT GAAACCCTGT
    CGCTCCCATG GAAAGAGTAA CCCGTACTGT GAGGTGACCA TGGGCTCCCA GTGTCACATC
    ACCAAGACGA TCCAGGACAC TCTGAATCCC AAGTGGAATT CCAACTGCCA GTTCTTCATC
    AGAGACCTGG AGCAGGAGGT GCTGTGCATC ACTGTGTTTG AGAGGGACCA GTTCTCGCCA
    GATGATTTTT TGGGTCGGAC GGAGATCCGC GTGGCCGACA TCAAGAAGGA CCAGGGCTCC
    AAGGGTCCCG TTACGAAGTG TCTCCTGCTG CACGAAGTTC CCACGGGAGA GATCGTGGTC
    CGCTTGGACC TGCAGTTGTT CGATGAGCCG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87631
    Clone ID Related Accession (Same CDS sequence) XM_015465187.1 , XM_015461917.1
    Accession Version XM_015465187.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5166bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product LOW QUALITY PROTEIN: intersectin-1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103793.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGGCTCAGT TTCCCACACC CTTTGGTGGC AGCCTGGATA TTTGGGCCAT AACTGTAGAG 
    GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT
    GGTGATCAAG CTAGAAACTT TTTTTTCNAA TCTGGGTTAC CTCAGCCTGT TTTAGCACAG
    ATATGGGCGC TCGCTGATAT GAATAATGAT GGAAGGATGG ATCAAGTGGA GTTTTCCATT
    GCTATGAAAC TCATCAAGCT GAAGCTCCAA GGATATCAGC TCCCCTCTGC ACTTCCCCCT
    GTCATGAAAC AGCAACCAGT TGCTATTTCT AGTGCACCAG CCTTTGGTAT GGGAGGTATT
    GCCAGCATGC CGCCGCTGAC CGCTGTTGCT CCTGTACCAA TGGGCTCCAT TCCCGTTGTT
    GGGATGTCTC CCCCACTAGT GTCATCTGTT CCTGCAGCAG GGGTGCCCCC CCTGGCCAAT
    GGGGCGCCCC CGGTCATACA GCCTCTGCCT GCATTTGCTC ATCCTGCTGC CACATTGCCA
    AAGAGTTCTT CCTTTAGTAG ATCTGGCCCA GGGTCGCAGT TAAACACAAA ATTACAGAAG
    GCACAATCAT TTGATGTGGC CAGTGTCCCT CCTACAGCTG AGTGGGCTGT TCCTCAGTCA
    TCCAGGCTGA AATACAGACA GCTCTTCAAT AGTCATGACA AAACCATGAG TGGACACCTA
    ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAATCAAGTT TACCACAGGC TCAGCTGGCT
    TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC
    CTGGCTATGC ACCTCATTGA TGTAGCTATG TCTGGTCAAC CACTGCCACC GGTCCTGCCC
    CCAGAATACA TTCCACCTTC CTTTAGAAGA GTTCGATCTG GTAGTGGTAT ATCTGTCATA
    ACCTCAACTT CGGTGGATCA GAGGTTGCCA GAGGAACCAG TTTTGGAAGA TGAACAGCAA
    CAACTAGAAA AGAAACTACC TGTAACCTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGG
    GGCAACCTGG AGCTGGAGAA GCGGAGACAA GCGCTCTTGG AGCAGCAGCG TAAAGAGCAG
    GAGCGCCTGG CCCAGCTGGA GCGGGCAGAG CAGGAGAGGA AGGAGCGGGA GCGCCAGGAG
    CAGGAGCGCA AAAGACAACT GGAGCTGGAA AAGCAGCTAG AGAAGCAGCG GGAGCTGGAG
    CGGCAGAGGG AGGAGGAGCG AAGGAAGGAA ATCGAGAGGC GGGAGGCTGC GAAACGGGAG
    CTTGAAAGGC AAAGACAGCT TGAATGGGAA CGGAATCGAA GACAAGAACT ATTAAATCAA
    AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT
    GAATTAGAAG CTCTAAATGA TAAAAAACAT CAATTAGAAG GAAAACTTCA GGATATCAGA
    TGTCGACTGA CAACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA
    ATTGCAGAAA TCACCCACCT ACAACAGCAA TTACAGGAGT CCCAGCAAAT GCTTGGAAGA
    CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTGAAAC AAGTTCAGCA AAACAGTTTG
    CACAGGGATT CACTTCTCAC ACTCAAAAGA GCCTTAGAAG CGAAAGAACT GGCTCGGCAG
    CAGCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GGTCAAAATT ACAGGAGATT
    GATATTTTCA ACAATCAGCT GAAGGAACTT AGAGAAATAC ATAATAAGCA GCAGCTCCAG
    AAACAGAAGT CCATAGAGGC TGAACGACTG AAACAGAAGG AACAAGAACG AAAGATCCTG
    GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CCAGCAGTGG
    CTGGAGCGCG TGCATCAGGA GGATGAGCAG CAGCGGCCAC GAAAACCCCA CGAGGAGGAG
    AAGCTCAAAA GGGAGGAGAG CGTCAAAAAG AAGGACAGTG AGGAGAAGGG CAAACCGGAA
    ATGCAAGACA AGCTGAGTCG GCTTTTCCAC CAGCATCAAG AACCAGCTAA GCCCGCTGTC
    CAGGCACCCT GGTCCACTGC AGAAAAAGGC CCACTTACGA TTTCTGCACA GGAGAATATA
    AAAGTGGTGT ATTACCGAGC ACTTTATCCC TTTGAGTCCA GGAGTCACGA TGAAATCACA
    ATCCAGCCGG GAGATATAGT CATGGTTAAA GGGGAATGGG TGGACGAAAG CCAAACTGGA
    GAACCGGGAT GGCTTGGAGG AGAATTAAAA GGAAAGACGG GATGGTTCCC TGCAAACTAC
    GCAGAGAAAA TCCCAGAAAA TGAGGTTCCC GCCCCAGTCA AACCAGTGAC TGAGGCGGCC
    TCCACCCCTA CACCCAAAGT GGCTGTGTGT GAGACCCCCG CCCCTTCATC CACAACCCCT
    GCAGAGCCCT CCGAGACCCC CAACAACTGG GCCGACTTCA GCTCCACGTG GCCCACCAGC
    ACGAATGAGA AACCAGAAAC CGACAGCTGG GACGCATGGG CAACCCAGCC CTCCCTCACC
    GTGCCAAGCG CCGGGCAGCT GAGGCAGAGG TCGGCCTTCA CTCCAGCCAC GGCCACGGGC
    TCATCTCCGT CTCCTGTTCT AGGCCAGGGT GAAAAGGTGG AGGGGCTACA GGCACAAGCC
    CTGTATCCGT GGAGAGCCAA GAAAGACAAC CATTTAAATT TTAACAAAAA CGACGTCATC
    ACCGTACTGG AGCAGCAAGA CATGTGGTGG TTTGGAGAAG TTCAAGGCCA GAAGGGTTGG
    TTCCCCAAGT CCTACGTGAA ACTCATTTCA GGGCCCATAA GGAAATCGAC AAGCATGGAT
    TCTGGTCCCT CAGAAAGTCC TGCAAGTCTA AAGAGGGTAG CTTCCCCAGC AGCCAAGCCA
    GCCGTGTCGG GAGAAGAATT TATTGCCATG TACACTTACG AGAGTTCTGA GCAAGGCGAT
    TTAACCTTTC AGCAAGGGGA TGTGATTTTG GTTACCAAGA AAGATGGTGA CTGGTGGACA
    GGAACAGTGG GCGACAAGTC CGGCGTCTTC CCTTCTAACT ATGTGAGGCT TAAAGATTCG
    GAGGGCTCTG GAACTGCTGG GAAAACAGGG AGTTTAGGAA AAAAACCTGA AATCGCGCAG
    GTCATTGCCT CCTACACAGC TACGGGTCCT GAACAGCTTA CCCTGGCCCC CGGTCAGCTG
    ATCCTGATCC GGAAAAAGAA CCCAGGTGGA TGGTGGGAAG GAGAGCTACA GGCACGTGGG
    AAAAAGCGCC AGATAGGCTG GTTTCCTGCT AATTACGTCA AACTTTTAAG CCCTGGGACA
    AGCAAAATCA CTCCAACAGA CCCACCGAAG CCAACAGCTT TCCCAACAGT GTGCCAGGTG
    ATCGGGATGT ACGACTACAC CGCGCAGAAC GACGACGAGC TGGCCTTCAG CAAGGGCCAG
    GTCATCAATG TCCTCAACAA GGAGGACCCC GACTGGTGGA AGGGTGAAGT CCACGGGCAG
    GTGGGGCTCT TCCCGTCCAA CTACGTGAAG TTGACCACGG ACACGGACCC CAGCCAGCAA
    TGGTGCTCAG ACTTACACCT CTTGGATATG CTGACCCCAA CTGAAAGAAA GCGACAGGGG
    TACATCCACG AGCTCATCGT CACGGAGGAG AACTACGTGA ATGACCTGCA GCTGGTCACA
    GAGATCTTTC AGAAACCCCT GATGGAGTCT GAGCTGCTGA CAGAAAAAGA GGGTGCTATG
    ATTTTTGTTA ACTGGAAGGA GCTGATTATG TGTAATATCA AACTACTGAA AGCGCTGAGG
    GTCCGCAAGA AGATGTCTGG GGAGAAGATG CCCGTGAAGA TGATCGGAGA CATCCTGACG
    GCCCAGCTGC CACACATGCA GCCCTACATC CGCTTCTGCA GCTGCCAGCT CAACGGGGCC
    GCCTTGATCC AGCAGAAGAC TGACGAGGCC CCAGACTTCA AGGAGTTTGT CAAAAGACTG
    GCCATGGACC CACGCTGTAA GGGAATGCCC CTGTCTAGTT TTATACTGAA GCCTATGCAA
    CGAGTAACGA GATACCCACT GATCATTAAA AATATCCTGG AAAACACTCC TGAAAACCAC
    CCAGACCACA GCCACCTGAA GCACGCCCTG GAGAAGGCGG AGGAGCTGTG CTCCCAGGTG
    AATGAAGGGG TGCGGGAGAA GGAGAACTCG GACCGACTGG AGTGGATCCA GGCCCACGTG
    CTGTGTGAAG GCTTGTCAGA GCAACTTGTG TTCAATTCCG TGACCAACTG CCTGGGGCCG
    CGCAAGTTCC TGCACAGTGG GAAGCTCTAC AAGGCCAAGA GCAACAAGGA GCTGTATGGC
    TTCCTCTTCA ACGACTTCCT GCTGCTGACC CAAATCATCA AGCCCCTGGG CTCCTCGGGC
    ACCGACAAAG TCTTCAGCCC CAAATCAAAC CTGCAGTATA AAATGTACAA AACACCTATT
    TTCCTAAATG AGGTTCTAGT GAAGTTGCCC ACTGACCCTT CTGGGGATGA GCCCATCTTC
    CACATTTCCC ACATTGACCG AGTCTACACC CTCCGAGCAG AGAGCATAAA TGAAAGGACT
    GCCTGGGTGC AGAAAATCAA AGCAGCGTCT GAACTCTACA TAGAGACAGA GAAGAAGAAG
    CGGGAGAAGG CGTACCTGGT CCGCTCCCAA AGAGCCACAG GCATCGGAAG GTTGATGGTG
    AACGTGGTTG AAGGCATTGA GCTGAAACCC TGTCGCTCCC ATGGAAAGAG TAACCCGTAC
    TGTGAGGTGA CCATGGGCTC CCAGTGTCAC ATCACCAAGA CGATCCAGGA CACTCTGAAT
    CCCAAGTGGA ATTCCAACTG CCAGTTCTTC ATCAGAGACC TGGAGCAGGA GGTGCTGTGC
    ATCACTGTGT TTGAGAGGGA CCAGTTCTCG CCAGATGATT TTTTGGGTCG GACGGAGATC
    CGCGTGGCCG ACATCAAGAA GGACCAGGGC TCCAAGGGTC CCGTTACGAA GTGTCTCCTG
    CTGCACGAAG TTCCCACGGG AGAGATCGTG GTCCGCTTGG ACCTGCAGTT GTTCGATGAG
    CCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320673.1
    CDS288..5453
    Translation

    Target ORF information:

    RefSeq Version XM_015465187.1
    Organism Bos taurus(cattle)
    Definition Bos taurus intersectin 1 (ITSN1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465187.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGGCTCAGT TTCCCACACC CTTTGGTGGC AGCCTGGATA TTTGGGCCAT AACTGTAGAG 
    GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT
    GGTGATCAAG CTAGAAACTT TTTTTTCNAA TCTGGGTTAC CTCAGCCTGT TTTAGCACAG
    ATATGGGCGC TCGCTGATAT GAATAATGAT GGAAGGATGG ATCAAGTGGA GTTTTCCATT
    GCTATGAAAC TCATCAAGCT GAAGCTCCAA GGATATCAGC TCCCCTCTGC ACTTCCCCCT
    GTCATGAAAC AGCAACCAGT TGCTATTTCT AGTGCACCAG CCTTTGGTAT GGGAGGTATT
    GCCAGCATGC CGCCGCTGAC CGCTGTTGCT CCTGTACCAA TGGGCTCCAT TCCCGTTGTT
    GGGATGTCTC CCCCACTAGT GTCATCTGTT CCTGCAGCAG GGGTGCCCCC CCTGGCCAAT
    GGGGCGCCCC CGGTCATACA GCCTCTGCCT GCATTTGCTC ATCCTGCTGC CACATTGCCA
    AAGAGTTCTT CCTTTAGTAG ATCTGGCCCA GGGTCGCAGT TAAACACAAA ATTACAGAAG
    GCACAATCAT TTGATGTGGC CAGTGTCCCT CCTACAGCTG AGTGGGCTGT TCCTCAGTCA
    TCCAGGCTGA AATACAGACA GCTCTTCAAT AGTCATGACA AAACCATGAG TGGACACCTA
    ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAATCAAGTT TACCACAGGC TCAGCTGGCT
    TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC
    CTGGCTATGC ACCTCATTGA TGTAGCTATG TCTGGTCAAC CACTGCCACC GGTCCTGCCC
    CCAGAATACA TTCCACCTTC CTTTAGAAGA GTTCGATCTG GTAGTGGTAT ATCTGTCATA
    ACCTCAACTT CGGTGGATCA GAGGTTGCCA GAGGAACCAG TTTTGGAAGA TGAACAGCAA
    CAACTAGAAA AGAAACTACC TGTAACCTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGG
    GGCAACCTGG AGCTGGAGAA GCGGAGACAA GCGCTCTTGG AGCAGCAGCG TAAAGAGCAG
    GAGCGCCTGG CCCAGCTGGA GCGGGCAGAG CAGGAGAGGA AGGAGCGGGA GCGCCAGGAG
    CAGGAGCGCA AAAGACAACT GGAGCTGGAA AAGCAGCTAG AGAAGCAGCG GGAGCTGGAG
    CGGCAGAGGG AGGAGGAGCG AAGGAAGGAA ATCGAGAGGC GGGAGGCTGC GAAACGGGAG
    CTTGAAAGGC AAAGACAGCT TGAATGGGAA CGGAATCGAA GACAAGAACT ATTAAATCAA
    AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT
    GAATTAGAAG CTCTAAATGA TAAAAAACAT CAATTAGAAG GAAAACTTCA GGATATCAGA
    TGTCGACTGA CAACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA
    ATTGCAGAAA TCACCCACCT ACAACAGCAA TTACAGGAGT CCCAGCAAAT GCTTGGAAGA
    CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTGAAAC AAGTTCAGCA AAACAGTTTG
    CACAGGGATT CACTTCTCAC ACTCAAAAGA GCCTTAGAAG CGAAAGAACT GGCTCGGCAG
    CAGCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GGTCAAAATT ACAGGAGATT
    GATATTTTCA ACAATCAGCT GAAGGAACTT AGAGAAATAC ATAATAAGCA GCAGCTCCAG
    AAACAGAAGT CCATAGAGGC TGAACGACTG AAACAGAAGG AACAAGAACG AAAGATCCTG
    GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CCAGCAGTGG
    CTGGAGCGCG TGCATCAGGA GGATGAGCAG CAGCGGCCAC GAAAACCCCA CGAGGAGGAG
    AAGCTCAAAA GGGAGGAGAG CGTCAAAAAG AAGGACAGTG AGGAGAAGGG CAAACCGGAA
    ATGCAAGACA AGCTGAGTCG GCTTTTCCAC CAGCATCAAG AACCAGCTAA GCCCGCTGTC
    CAGGCACCCT GGTCCACTGC AGAAAAAGGC CCACTTACGA TTTCTGCACA GGAGAATATA
    AAAGTGGTGT ATTACCGAGC ACTTTATCCC TTTGAGTCCA GGAGTCACGA TGAAATCACA
    ATCCAGCCGG GAGATATAGT CATGGTTAAA GGGGAATGGG TGGACGAAAG CCAAACTGGA
    GAACCGGGAT GGCTTGGAGG AGAATTAAAA GGAAAGACGG GATGGTTCCC TGCAAACTAC
    GCAGAGAAAA TCCCAGAAAA TGAGGTTCCC GCCCCAGTCA AACCAGTGAC TGAGGCGGCC
    TCCACCCCTA CACCCAAAGT GGCTGTGTGT GAGACCCCCG CCCCTTCATC CACAACCCCT
    GCAGAGCCCT CCGAGACCCC CAACAACTGG GCCGACTTCA GCTCCACGTG GCCCACCAGC
    ACGAATGAGA AACCAGAAAC CGACAGCTGG GACGCATGGG CAACCCAGCC CTCCCTCACC
    GTGCCAAGCG CCGGGCAGCT GAGGCAGAGG TCGGCCTTCA CTCCAGCCAC GGCCACGGGC
    TCATCTCCGT CTCCTGTTCT AGGCCAGGGT GAAAAGGTGG AGGGGCTACA GGCACAAGCC
    CTGTATCCGT GGAGAGCCAA GAAAGACAAC CATTTAAATT TTAACAAAAA CGACGTCATC
    ACCGTACTGG AGCAGCAAGA CATGTGGTGG TTTGGAGAAG TTCAAGGCCA GAAGGGTTGG
    TTCCCCAAGT CCTACGTGAA ACTCATTTCA GGGCCCATAA GGAAATCGAC AAGCATGGAT
    TCTGGTCCCT CAGAAAGTCC TGCAAGTCTA AAGAGGGTAG CTTCCCCAGC AGCCAAGCCA
    GCCGTGTCGG GAGAAGAATT TATTGCCATG TACACTTACG AGAGTTCTGA GCAAGGCGAT
    TTAACCTTTC AGCAAGGGGA TGTGATTTTG GTTACCAAGA AAGATGGTGA CTGGTGGACA
    GGAACAGTGG GCGACAAGTC CGGCGTCTTC CCTTCTAACT ATGTGAGGCT TAAAGATTCG
    GAGGGCTCTG GAACTGCTGG GAAAACAGGG AGTTTAGGAA AAAAACCTGA AATCGCGCAG
    GTCATTGCCT CCTACACAGC TACGGGTCCT GAACAGCTTA CCCTGGCCCC CGGTCAGCTG
    ATCCTGATCC GGAAAAAGAA CCCAGGTGGA TGGTGGGAAG GAGAGCTACA GGCACGTGGG
    AAAAAGCGCC AGATAGGCTG GTTTCCTGCT AATTACGTCA AACTTTTAAG CCCTGGGACA
    AGCAAAATCA CTCCAACAGA CCCACCGAAG CCAACAGCTT TCCCAACAGT GTGCCAGGTG
    ATCGGGATGT ACGACTACAC CGCGCAGAAC GACGACGAGC TGGCCTTCAG CAAGGGCCAG
    GTCATCAATG TCCTCAACAA GGAGGACCCC GACTGGTGGA AGGGTGAAGT CCACGGGCAG
    GTGGGGCTCT TCCCGTCCAA CTACGTGAAG TTGACCACGG ACACGGACCC CAGCCAGCAA
    TGGTGCTCAG ACTTACACCT CTTGGATATG CTGACCCCAA CTGAAAGAAA GCGACAGGGG
    TACATCCACG AGCTCATCGT CACGGAGGAG AACTACGTGA ATGACCTGCA GCTGGTCACA
    GAGATCTTTC AGAAACCCCT GATGGAGTCT GAGCTGCTGA CAGAAAAAGA GGGTGCTATG
    ATTTTTGTTA ACTGGAAGGA GCTGATTATG TGTAATATCA AACTACTGAA AGCGCTGAGG
    GTCCGCAAGA AGATGTCTGG GGAGAAGATG CCCGTGAAGA TGATCGGAGA CATCCTGACG
    GCCCAGCTGC CACACATGCA GCCCTACATC CGCTTCTGCA GCTGCCAGCT CAACGGGGCC
    GCCTTGATCC AGCAGAAGAC TGACGAGGCC CCAGACTTCA AGGAGTTTGT CAAAAGACTG
    GCCATGGACC CACGCTGTAA GGGAATGCCC CTGTCTAGTT TTATACTGAA GCCTATGCAA
    CGAGTAACGA GATACCCACT GATCATTAAA AATATCCTGG AAAACACTCC TGAAAACCAC
    CCAGACCACA GCCACCTGAA GCACGCCCTG GAGAAGGCGG AGGAGCTGTG CTCCCAGGTG
    AATGAAGGGG TGCGGGAGAA GGAGAACTCG GACCGACTGG AGTGGATCCA GGCCCACGTG
    CTGTGTGAAG GCTTGTCAGA GCAACTTGTG TTCAATTCCG TGACCAACTG CCTGGGGCCG
    CGCAAGTTCC TGCACAGTGG GAAGCTCTAC AAGGCCAAGA GCAACAAGGA GCTGTATGGC
    TTCCTCTTCA ACGACTTCCT GCTGCTGACC CAAATCATCA AGCCCCTGGG CTCCTCGGGC
    ACCGACAAAG TCTTCAGCCC CAAATCAAAC CTGCAGTATA AAATGTACAA AACACCTATT
    TTCCTAAATG AGGTTCTAGT GAAGTTGCCC ACTGACCCTT CTGGGGATGA GCCCATCTTC
    CACATTTCCC ACATTGACCG AGTCTACACC CTCCGAGCAG AGAGCATAAA TGAAAGGACT
    GCCTGGGTGC AGAAAATCAA AGCAGCGTCT GAACTCTACA TAGAGACAGA GAAGAAGAAG
    CGGGAGAAGG CGTACCTGGT CCGCTCCCAA AGAGCCACAG GCATCGGAAG GTTGATGGTG
    AACGTGGTTG AAGGCATTGA GCTGAAACCC TGTCGCTCCC ATGGAAAGAG TAACCCGTAC
    TGTGAGGTGA CCATGGGCTC CCAGTGTCAC ATCACCAAGA CGATCCAGGA CACTCTGAAT
    CCCAAGTGGA ATTCCAACTG CCAGTTCTTC ATCAGAGACC TGGAGCAGGA GGTGCTGTGC
    ATCACTGTGT TTGAGAGGGA CCAGTTCTCG CCAGATGATT TTTTGGGTCG GACGGAGATC
    CGCGTGGCCG ACATCAAGAA GGACCAGGGC TCCAAGGGTC CCGTTACGAA GTGTCTCCTG
    CTGCACGAAG TTCCCACGGG AGAGATCGTG GTCCGCTTGG ACCTGCAGTT GTTCGATGAG
    CCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87631
    Clone ID Related Accession (Same CDS sequence) XM_015465187.1 , XM_015461917.1
    Accession Version XM_015461917.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5166bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product LOW QUALITY PROTEIN: intersectin-1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644478.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGGCTCAGT TTCCCACACC CTTTGGTGGC AGCCTGGATA TTTGGGCCAT AACTGTAGAG 
    GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT
    GGTGATCAAG CTAGAAACTT TTTTTTCNAA TCTGGGTTAC CTCAGCCTGT TTTAGCACAG
    ATATGGGCGC TCGCTGATAT GAATAATGAT GGAAGGATGG ATCAAGTGGA GTTTTCCATT
    GCTATGAAAC TCATCAAGCT GAAGCTCCAA GGATATCAGC TCCCCTCTGC ACTTCCCCCT
    GTCATGAAAC AGCAACCAGT TGCTATTTCT AGTGCACCAG CCTTTGGTAT GGGAGGTATT
    GCCAGCATGC CGCCGCTGAC CGCTGTTGCT CCTGTACCAA TGGGCTCCAT TCCCGTTGTT
    GGGATGTCTC CCCCACTAGT GTCATCTGTT CCTGCAGCAG GGGTGCCCCC CCTGGCCAAT
    GGGGCGCCCC CGGTCATACA GCCTCTGCCT GCATTTGCTC ATCCTGCTGC CACATTGCCA
    AAGAGTTCTT CCTTTAGTAG ATCTGGCCCA GGGTCGCAGT TAAACACAAA ATTACAGAAG
    GCACAATCAT TTGATGTGGC CAGTGTCCCT CCTACAGCTG AGTGGGCTGT TCCTCAGTCA
    TCCAGGCTGA AATACAGACA GCTCTTCAAT AGTCATGACA AAACCATGAG TGGACACCTA
    ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAATCAAGTT TACCACAGGC TCAGCTGGCT
    TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC
    CTGGCTATGC ACCTCATTGA TGTAGCTATG TCTGGTCAAC CACTGCCACC GGTCCTGCCC
    CCAGAATACA TTCCACCTTC CTTTAGAAGA GTTCGATCTG GTAGTGGTAT ATCTGTCATA
    ACCTCAACTT CGGTGGATCA GAGGTTGCCA GAGGAACCAG TTTTGGAAGA TGAACAGCAA
    CAACTAGAAA AGAAACTACC TGTAACCTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGG
    GGCAACCTGG AGCTGGAGAA GCGGAGACAA GCGCTCTTGG AGCAGCAGCG TAAAGAGCAG
    GAGCGCCTGG CCCAGCTGGA GCGGGCAGAG CAGGAGAGGA AGGAGCGGGA GCGCCAGGAG
    CAGGAGCGCA AAAGACAACT GGAGCTGGAA AAGCAGCTAG AGAAGCAGCG GGAGCTGGAG
    CGGCAGAGGG AGGAGGAGCG AAGGAAGGAA ATCGAGAGGC GGGAGGCTGC GAAACGGGAG
    CTTGAAAGGC AAAGACAGCT TGAATGGGAA CGGAATCGAA GACAAGAACT ATTAAATCAA
    AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT
    GAATTAGAAG CTCTAAATGA TAAAAAACAT CAATTAGAAG GAAAACTTCA GGATATCAGA
    TGTCGACTGA CAACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA
    ATTGCAGAAA TCACCCACCT ACAACAGCAA TTACAGGAGT CCCAGCAAAT GCTTGGAAGA
    CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTGAAAC AAGTTCAGCA AAACAGTTTG
    CACAGGGATT CACTTCTCAC ACTCAAAAGA GCCTTAGAAG CGAAAGAACT GGCTCGGCAG
    CAGCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GGTCAAAATT ACAGGAGATT
    GATATTTTCA ACAATCAGCT GAAGGAACTT AGAGAAATAC ATAATAAGCA GCAGCTCCAG
    AAACAGAAGT CCATAGAGGC TGAACGACTG AAACAGAAGG AACAAGAACG AAAGATCCTG
    GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CCAGCAGTGG
    CTGGAGCGCG TGCATCAGGA GGATGAGCAG CAGCGGCCAC GAAAACCCCA CGAGGAGGAG
    AAGCTCAAAA GGGAGGAGAG CGTCAAAAAG AAGGACAGTG AGGAGAAGGG CAAACCGGAA
    ATGCAAGACA AGCTGAGTCG GCTTTTCCAC CAGCATCAAG AACCAGCTAA GCCCGCTGTC
    CAGGCACCCT GGTCCACTGC AGAAAAAGGC CCACTTACGA TTTCTGCACA GGAGAATATA
    AAAGTGGTGT ATTACCGAGC ACTTTATCCC TTTGAGTCCA GGAGTCACGA TGAAATCACA
    ATCCAGCCGG GAGATATAGT CATGGTTAAA GGGGAATGGG TGGACGAAAG CCAAACTGGA
    GAACCGGGAT GGCTTGGAGG AGAATTAAAA GGAAAGACGG GATGGTTCCC TGCAAACTAC
    GCAGAGAAAA TCCCAGAAAA TGAGGTTCCC GCCCCAGTCA AACCAGTGAC TGAGGCGGCC
    TCCACCCCTA CACCCAAAGT GGCTGTGTGT GAGACCCCCG CCCCTTCATC CACAACCCCT
    GCAGAGCCCT CCGAGACCCC CAACAACTGG GCCGACTTCA GCTCCACGTG GCCCACCAGC
    ACGAATGAGA AACCAGAAAC CGACAGCTGG GACGCATGGG CAACCCAGCC CTCCCTCACC
    GTGCCAAGCG CCGGGCAGCT GAGGCAGAGG TCGGCCTTCA CTCCAGCCAC GGCCACGGGC
    TCATCTCCGT CTCCTGTTCT AGGCCAGGGT GAAAAGGTGG AGGGGCTACA GGCACAAGCC
    CTGTATCCGT GGAGAGCCAA GAAAGACAAC CATTTAAATT TTAACAAAAA CGACGTCATC
    ACCGTACTGG AGCAGCAAGA CATGTGGTGG TTTGGAGAAG TTCAAGGCCA GAAGGGTTGG
    TTCCCCAAGT CCTACGTGAA ACTCATTTCA GGGCCCATAA GGAAATCGAC AAGCATGGAT
    TCTGGTCCCT CAGAAAGTCC TGCAAGTCTA AAGAGGGTAG CTTCCCCAGC AGCCAAGCCA
    GCCGTGTCGG GAGAAGAATT TATTGCCATG TACACTTACG AGAGTTCTGA GCAAGGCGAT
    TTAACCTTTC AGCAAGGGGA TGTGATTTTG GTTACCAAGA AAGATGGTGA CTGGTGGACA
    GGAACAGTGG GCGACAAGTC CGGCGTCTTC CCTTCTAACT ATGTGAGGCT TAAAGATTCG
    GAGGGCTCTG GAACTGCTGG GAAAACAGGG AGTTTAGGAA AAAAACCTGA AATCGCGCAG
    GTCATTGCCT CCTACACAGC TACGGGTCCT GAACAGCTTA CCCTGGCCCC CGGTCAGCTG
    ATCCTGATCC GGAAAAAGAA CCCAGGTGGA TGGTGGGAAG GAGAGCTACA GGCACGTGGG
    AAAAAGCGCC AGATAGGCTG GTTTCCTGCT AATTACGTCA AACTTTTAAG CCCTGGGACA
    AGCAAAATCA CTCCAACAGA CCCACCGAAG CCAACAGCTT TCCCAACAGT GTGCCAGGTG
    ATCGGGATGT ACGACTACAC CGCGCAGAAC GACGACGAGC TGGCCTTCAG CAAGGGCCAG
    GTCATCAATG TCCTCAACAA GGAGGACCCC GACTGGTGGA AGGGTGAAGT CCACGGGCAG
    GTGGGGCTCT TCCCGTCCAA CTACGTGAAG TTGACCACGG ACACGGACCC CAGCCAGCAA
    TGGTGCTCAG ACTTACACCT CTTGGATATG CTGACCCCAA CTGAAAGAAA GCGACAGGGG
    TACATCCACG AGCTCATCGT CACGGAGGAG AACTACGTGA ATGACCTGCA GCTGGTCACA
    GAGATCTTTC AGAAACCCCT GATGGAGTCT GAGCTGCTGA CAGAAAAAGA GGGTGCTATG
    ATTTTTGTTA ACTGGAAGGA GCTGATTATG TGTAATATCA AACTACTGAA AGCGCTGAGG
    GTCCGCAAGA AGATGTCTGG GGAGAAGATG CCCGTGAAGA TGATCGGAGA CATCCTGACG
    GCCCAGCTGC CACACATGCA GCCCTACATC CGCTTCTGCA GCTGCCAGCT CAACGGGGCC
    GCCTTGATCC AGCAGAAGAC TGACGAGGCC CCAGACTTCA AGGAGTTTGT CAAAAGACTG
    GCCATGGACC CACGCTGTAA GGGAATGCCC CTGTCTAGTT TTATACTGAA GCCTATGCAA
    CGAGTAACGA GATACCCACT GATCATTAAA AATATCCTGG AAAACACTCC TGAAAACCAC
    CCAGACCACA GCCACCTGAA GCACGCCCTG GAGAAGGCGG AGGAGCTGTG CTCCCAGGTG
    AATGAAGGGG TGCGGGAGAA GGAGAACTCG GACCGACTGG AGTGGATCCA GGCCCACGTG
    CTGTGTGAAG GCTTGTCAGA GCAACTTGTG TTCAATTCCG TGACCAACTG CCTGGGGCCG
    CGCAAGTTCC TGCACAGTGG GAAGCTCTAC AAGGCCAAGA GCAACAAGGA GCTGTATGGC
    TTCCTCTTCA ACGACTTCCT GCTGCTGACC CAAATCATCA AGCCCCTGGG CTCCTCGGGC
    ACCGACAAAG TCTTCAGCCC CAAATCAAAC CTGCAGTATA AAATGTACAA AACACCTATT
    TTCCTAAATG AGGTTCTAGT GAAGTTGCCC ACTGACCCTT CTGGGGATGA GCCCATCTTC
    CACATTTCCC ACATTGACCG AGTCTACACC CTCCGAGCAG AGAGCATAAA TGAAAGGACT
    GCCTGGGTGC AGAAAATCAA AGCAGCGTCT GAACTCTACA TAGAGACAGA GAAGAAGAAG
    CGGGAGAAGG CGTACCTGGT CCGCTCCCAA AGAGCCACAG GCATCGGAAG GTTGATGGTG
    AACGTGGTTG AAGGCATTGA GCTGAAACCC TGTCGCTCCC ATGGAAAGAG TAACCCGTAC
    TGTGAGGTGA CCATGGGCTC CCAGTGTCAC ATCACCAAGA CGATCCAGGA CACTCTGAAT
    CCCAAGTGGA ATTCCAACTG CCAGTTCTTC ATCAGAGACC TGGAGCAGGA GGTGCTGTGC
    ATCACTGTGT TTGAGAGGGA CCAGTTCTCG CCAGATGATT TTTTGGGTCG GACGGAGATC
    CGCGTGGCCG ACATCAAGAA GGACCAGGGC TCCAAGGGTC CCGTTACGAA GTGTCTCCTG
    CTGCACGAAG TTCCCACGGG AGAGATCGTG GTCCGCTTGG ACCTGCAGTT GTTCGATGAG
    CCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317403.1
    CDS288..5453
    Translation

    Target ORF information:

    RefSeq Version XM_015461917.1
    Organism Bos taurus(cattle)
    Definition Bos taurus intersectin 1 (ITSN1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015461917.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGGCTCAGT TTCCCACACC CTTTGGTGGC AGCCTGGATA TTTGGGCCAT AACTGTAGAG 
    GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT
    GGTGATCAAG CTAGAAACTT TTTTTTCNAA TCTGGGTTAC CTCAGCCTGT TTTAGCACAG
    ATATGGGCGC TCGCTGATAT GAATAATGAT GGAAGGATGG ATCAAGTGGA GTTTTCCATT
    GCTATGAAAC TCATCAAGCT GAAGCTCCAA GGATATCAGC TCCCCTCTGC ACTTCCCCCT
    GTCATGAAAC AGCAACCAGT TGCTATTTCT AGTGCACCAG CCTTTGGTAT GGGAGGTATT
    GCCAGCATGC CGCCGCTGAC CGCTGTTGCT CCTGTACCAA TGGGCTCCAT TCCCGTTGTT
    GGGATGTCTC CCCCACTAGT GTCATCTGTT CCTGCAGCAG GGGTGCCCCC CCTGGCCAAT
    GGGGCGCCCC CGGTCATACA GCCTCTGCCT GCATTTGCTC ATCCTGCTGC CACATTGCCA
    AAGAGTTCTT CCTTTAGTAG ATCTGGCCCA GGGTCGCAGT TAAACACAAA ATTACAGAAG
    GCACAATCAT TTGATGTGGC CAGTGTCCCT CCTACAGCTG AGTGGGCTGT TCCTCAGTCA
    TCCAGGCTGA AATACAGACA GCTCTTCAAT AGTCATGACA AAACCATGAG TGGACACCTA
    ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAATCAAGTT TACCACAGGC TCAGCTGGCT
    TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC
    CTGGCTATGC ACCTCATTGA TGTAGCTATG TCTGGTCAAC CACTGCCACC GGTCCTGCCC
    CCAGAATACA TTCCACCTTC CTTTAGAAGA GTTCGATCTG GTAGTGGTAT ATCTGTCATA
    ACCTCAACTT CGGTGGATCA GAGGTTGCCA GAGGAACCAG TTTTGGAAGA TGAACAGCAA
    CAACTAGAAA AGAAACTACC TGTAACCTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGG
    GGCAACCTGG AGCTGGAGAA GCGGAGACAA GCGCTCTTGG AGCAGCAGCG TAAAGAGCAG
    GAGCGCCTGG CCCAGCTGGA GCGGGCAGAG CAGGAGAGGA AGGAGCGGGA GCGCCAGGAG
    CAGGAGCGCA AAAGACAACT GGAGCTGGAA AAGCAGCTAG AGAAGCAGCG GGAGCTGGAG
    CGGCAGAGGG AGGAGGAGCG AAGGAAGGAA ATCGAGAGGC GGGAGGCTGC GAAACGGGAG
    CTTGAAAGGC AAAGACAGCT TGAATGGGAA CGGAATCGAA GACAAGAACT ATTAAATCAA
    AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT
    GAATTAGAAG CTCTAAATGA TAAAAAACAT CAATTAGAAG GAAAACTTCA GGATATCAGA
    TGTCGACTGA CAACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA
    ATTGCAGAAA TCACCCACCT ACAACAGCAA TTACAGGAGT CCCAGCAAAT GCTTGGAAGA
    CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTGAAAC AAGTTCAGCA AAACAGTTTG
    CACAGGGATT CACTTCTCAC ACTCAAAAGA GCCTTAGAAG CGAAAGAACT GGCTCGGCAG
    CAGCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GGTCAAAATT ACAGGAGATT
    GATATTTTCA ACAATCAGCT GAAGGAACTT AGAGAAATAC ATAATAAGCA GCAGCTCCAG
    AAACAGAAGT CCATAGAGGC TGAACGACTG AAACAGAAGG AACAAGAACG AAAGATCCTG
    GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CCAGCAGTGG
    CTGGAGCGCG TGCATCAGGA GGATGAGCAG CAGCGGCCAC GAAAACCCCA CGAGGAGGAG
    AAGCTCAAAA GGGAGGAGAG CGTCAAAAAG AAGGACAGTG AGGAGAAGGG CAAACCGGAA
    ATGCAAGACA AGCTGAGTCG GCTTTTCCAC CAGCATCAAG AACCAGCTAA GCCCGCTGTC
    CAGGCACCCT GGTCCACTGC AGAAAAAGGC CCACTTACGA TTTCTGCACA GGAGAATATA
    AAAGTGGTGT ATTACCGAGC ACTTTATCCC TTTGAGTCCA GGAGTCACGA TGAAATCACA
    ATCCAGCCGG GAGATATAGT CATGGTTAAA GGGGAATGGG TGGACGAAAG CCAAACTGGA
    GAACCGGGAT GGCTTGGAGG AGAATTAAAA GGAAAGACGG GATGGTTCCC TGCAAACTAC
    GCAGAGAAAA TCCCAGAAAA TGAGGTTCCC GCCCCAGTCA AACCAGTGAC TGAGGCGGCC
    TCCACCCCTA CACCCAAAGT GGCTGTGTGT GAGACCCCCG CCCCTTCATC CACAACCCCT
    GCAGAGCCCT CCGAGACCCC CAACAACTGG GCCGACTTCA GCTCCACGTG GCCCACCAGC
    ACGAATGAGA AACCAGAAAC CGACAGCTGG GACGCATGGG CAACCCAGCC CTCCCTCACC
    GTGCCAAGCG CCGGGCAGCT GAGGCAGAGG TCGGCCTTCA CTCCAGCCAC GGCCACGGGC
    TCATCTCCGT CTCCTGTTCT AGGCCAGGGT GAAAAGGTGG AGGGGCTACA GGCACAAGCC
    CTGTATCCGT GGAGAGCCAA GAAAGACAAC CATTTAAATT TTAACAAAAA CGACGTCATC
    ACCGTACTGG AGCAGCAAGA CATGTGGTGG TTTGGAGAAG TTCAAGGCCA GAAGGGTTGG
    TTCCCCAAGT CCTACGTGAA ACTCATTTCA GGGCCCATAA GGAAATCGAC AAGCATGGAT
    TCTGGTCCCT CAGAAAGTCC TGCAAGTCTA AAGAGGGTAG CTTCCCCAGC AGCCAAGCCA
    GCCGTGTCGG GAGAAGAATT TATTGCCATG TACACTTACG AGAGTTCTGA GCAAGGCGAT
    TTAACCTTTC AGCAAGGGGA TGTGATTTTG GTTACCAAGA AAGATGGTGA CTGGTGGACA
    GGAACAGTGG GCGACAAGTC CGGCGTCTTC CCTTCTAACT ATGTGAGGCT TAAAGATTCG
    GAGGGCTCTG GAACTGCTGG GAAAACAGGG AGTTTAGGAA AAAAACCTGA AATCGCGCAG
    GTCATTGCCT CCTACACAGC TACGGGTCCT GAACAGCTTA CCCTGGCCCC CGGTCAGCTG
    ATCCTGATCC GGAAAAAGAA CCCAGGTGGA TGGTGGGAAG GAGAGCTACA GGCACGTGGG
    AAAAAGCGCC AGATAGGCTG GTTTCCTGCT AATTACGTCA AACTTTTAAG CCCTGGGACA
    AGCAAAATCA CTCCAACAGA CCCACCGAAG CCAACAGCTT TCCCAACAGT GTGCCAGGTG
    ATCGGGATGT ACGACTACAC CGCGCAGAAC GACGACGAGC TGGCCTTCAG CAAGGGCCAG
    GTCATCAATG TCCTCAACAA GGAGGACCCC GACTGGTGGA AGGGTGAAGT CCACGGGCAG
    GTGGGGCTCT TCCCGTCCAA CTACGTGAAG TTGACCACGG ACACGGACCC CAGCCAGCAA
    TGGTGCTCAG ACTTACACCT CTTGGATATG CTGACCCCAA CTGAAAGAAA GCGACAGGGG
    TACATCCACG AGCTCATCGT CACGGAGGAG AACTACGTGA ATGACCTGCA GCTGGTCACA
    GAGATCTTTC AGAAACCCCT GATGGAGTCT GAGCTGCTGA CAGAAAAAGA GGGTGCTATG
    ATTTTTGTTA ACTGGAAGGA GCTGATTATG TGTAATATCA AACTACTGAA AGCGCTGAGG
    GTCCGCAAGA AGATGTCTGG GGAGAAGATG CCCGTGAAGA TGATCGGAGA CATCCTGACG
    GCCCAGCTGC CACACATGCA GCCCTACATC CGCTTCTGCA GCTGCCAGCT CAACGGGGCC
    GCCTTGATCC AGCAGAAGAC TGACGAGGCC CCAGACTTCA AGGAGTTTGT CAAAAGACTG
    GCCATGGACC CACGCTGTAA GGGAATGCCC CTGTCTAGTT TTATACTGAA GCCTATGCAA
    CGAGTAACGA GATACCCACT GATCATTAAA AATATCCTGG AAAACACTCC TGAAAACCAC
    CCAGACCACA GCCACCTGAA GCACGCCCTG GAGAAGGCGG AGGAGCTGTG CTCCCAGGTG
    AATGAAGGGG TGCGGGAGAA GGAGAACTCG GACCGACTGG AGTGGATCCA GGCCCACGTG
    CTGTGTGAAG GCTTGTCAGA GCAACTTGTG TTCAATTCCG TGACCAACTG CCTGGGGCCG
    CGCAAGTTCC TGCACAGTGG GAAGCTCTAC AAGGCCAAGA GCAACAAGGA GCTGTATGGC
    TTCCTCTTCA ACGACTTCCT GCTGCTGACC CAAATCATCA AGCCCCTGGG CTCCTCGGGC
    ACCGACAAAG TCTTCAGCCC CAAATCAAAC CTGCAGTATA AAATGTACAA AACACCTATT
    TTCCTAAATG AGGTTCTAGT GAAGTTGCCC ACTGACCCTT CTGGGGATGA GCCCATCTTC
    CACATTTCCC ACATTGACCG AGTCTACACC CTCCGAGCAG AGAGCATAAA TGAAAGGACT
    GCCTGGGTGC AGAAAATCAA AGCAGCGTCT GAACTCTACA TAGAGACAGA GAAGAAGAAG
    CGGGAGAAGG CGTACCTGGT CCGCTCCCAA AGAGCCACAG GCATCGGAAG GTTGATGGTG
    AACGTGGTTG AAGGCATTGA GCTGAAACCC TGTCGCTCCC ATGGAAAGAG TAACCCGTAC
    TGTGAGGTGA CCATGGGCTC CCAGTGTCAC ATCACCAAGA CGATCCAGGA CACTCTGAAT
    CCCAAGTGGA ATTCCAACTG CCAGTTCTTC ATCAGAGACC TGGAGCAGGA GGTGCTGTGC
    ATCACTGTGT TTGAGAGGGA CCAGTTCTCG CCAGATGATT TTTTGGGTCG GACGGAGATC
    CGCGTGGCCG ACATCAAGAA GGACCAGGGC TCCAAGGGTC CCGTTACGAA GTGTCTCCTG
    CTGCACGAAG TTCCCACGGG AGAGATCGTG GTCCGCTTGG ACCTGCAGTT GTTCGATGAG
    CCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.