GAREM1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	GAREM1
Entrez Gene ID	507330
Full Name	GRB2 associated regulator of MAPK1 subtype 1
Synonyms	FAM59A,GAREM
General protein information	Preferred Names GRB2 associated regulator of MAPK1 subtype 1 Names GRB2-associated and regulator of MAPK protein 1 GRB2-associated and regulator of MAPK protein GRB2 associated, regulator of MAPK1 Grb2-associated and regulator of Erk/MAPK family with sequence similarity 59, member A
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005224060.4	XP_005224117.1	GRB2-associated and regulator of MAPK protein 1 isoform X1
XM_015469095.1	XP_015324581.1	GRB2-associated and regulator of MAPK protein isoform X2
XM_002697684.4	XP_002697730.1	GRB2-associated and regulator of MAPK protein isoform X2
XM_015469096.1	XP_015324582.1	GRB2-associated and regulator of MAPK protein isoform X3
XM_015469094.1	XP_015324580.1	GRB2-associated and regulator of MAPK protein isoform X1
XM_005224060.3	XP_005224117.1	GRB2-associated and regulator of MAPK protein isoform X1
XM_002697684.5	XP_002697730.1	GRB2-associated and regulator of MAPK protein 1 isoform X2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Garem	NP_001028617.2
Xenopus tropicalis (tropical clawed frog)	garem	XP_004915258.1
Homo sapiens (human)	GAREM	NP_001229338.1
Pan troglodytes (chimpanzee)	FAM59A	XP_524002.3
Canis lupus familiaris (dog)	FAM59A	XP_005623042.1
Rattus norvegicus (Norway rat)	Garem	XP_006254547.1
Gallus gallus (chicken)	FAM59A	XP_419182.2
Danio rerio (zebrafish)	garem	NP_956463.1
Bos taurus (cattle)	GAREM	XP_005224117.1

Gene Ontology

The following GAREM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GAREM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa32452	XM_015469094.1 Latest version!	Bos taurus GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa32452	XM_005224060.4 Latest version!	Bos taurus GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa32453	XM_002697684.5 Latest version!	Bos taurus GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa32453	XM_015469095.1 Latest version!	Bos taurus GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa95180	XM_015469096.1 Latest version!	Bos taurus GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OBa32452	XM_005224060.3	Bos taurus GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa32453	XM_002697684.4	Bos taurus GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GAREM1 ( XM_005224060.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005224117.1 Bos taurus GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa32452
Clone ID Related Accession (Same CDS sequence)	XM_005224060.4 , XM_015469094.1 , XM_005224060.3
Accession Version	XM_005224060.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2619bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	GRB2-associated and regulator of MAPK protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005224060.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCGG CGCCCTCGCT GGGCTGCAGC CTCAAGGATG TGAAGTGGAG CTCGGTGGCC 
GTTCCGCTCG ACCTGCTGGT CAGCACTTAC CGACTGCCCC AGATTGCGCG GCTGGACAGC 
GGAGAGTGCG TAGAAGGGCT GCGGGACAAT GACTACCTTC TGATCCACTC GTGTCGTCAG 
TGGACCACCA TCACCGCCCA CAGCTTGGAG GAGGGGCACT ATGTCATTGG GCCAAAGATA 
GAGATTCCGG TACATTATGC AGGGCAATTC AAGTTGCTGG AACAAGACCG AGATATAAAG 
GAGCCAGTGC AATATTTCAA CAGCGTGGAG GAGGTGGCCA AAGCATTTCC TGAACGCGTG 
TACGTCATGG AGGAAATAAC ATTCAACGTG AAGGTTGCTT CGGGTGAATG CAATGAAGAC 
ACCGAAGTTT ACAACATCAC CCTGTGTACG GGAGATGAAC TCACTCTAAT GGGCCAGGCA 
GAAATCCTTT ATGCAAAGAC TTTCAAGGAG AAGTCGCGAC TCAACACCAT CTTCAAAAAG 
ATTGGGAAAC TCAACTCCAT CAGCAAGCTG GGAAAAGGCA AGATGCCCTG CCTCATTTGC 
ATGAATCACC GGACCAACGA GAGCATCAGC CTCCCGTTCC AGTGCAAGGG CAGGTTTAGC 
ACCCGGAGCC CCCTGGAGCT TCAGATGCAG GAGGGCGAGC ATACCATCCG CAACATCGTG 
GAGAAAACCA GGCTCCCCGT GAACGTGACG GTGCCCAGCC CACCGCCCAG AAACCCGTAT 
GACCTCCACT TCATCCGCGA GGGGCACCGC TACAAGTTTG TGAACATCCA GACCAAGACA 
GTGGTGGTCT GCTGTGTGCT GCGGAACAAC AAGATCCTCC CTATGCACTT CCCTTTGCAC 
TTGACCGTCC CCAAGTTTAG CCTCCCGGAA CACCTGGTCA AGGGAGAGGG ATGGCCTGAA 
ACCCTGGTCC ATCACTGGCT GGGTATCTGC CAAGAACAGT TCGACATCGA TGAGTATTCA 
CGGGCAGTCC GCGACGTGAA AACCGACTGG AACGAGGACT GTAAGAGCCC CAAGAAAGGC 
CGGTGTGCCG GCCATAACCA CGTGCCCAAT TCCCTTAGCT ACGCTCGCGA TGAGCTCACC 
CAGTCCTTCC ACCGGCTCTC GGTCTGTGTG TATGGCAACA ATCTCCATGG CAACAGCGAG 
GTGAACCTCC ACGGCTGCAG GGACCTGGGA GGAGAGTGGG CCCCCTTTCC TCACGACATC 
CTACCCTATC AGGACTCGGG GGACAGCGGG AGCGACTACC TTTTCCCAGA AGCTGGTGAA 
GAATCTGCAG GCATGGCAGG GAAGTCAGAA CTTCCTTATG AAGAGCTGTG GCTGGAGGAA 
GGCAAGCCCA GCCATCAGCC TCTCACTCGC TCCCTGAGCG AGAAGAGCAG ATGCGACCAG 
TTTCGAGGTT CTGTCCGGTC CAAGTGTGCA ACCTCTCCCC TTCCTGTGCC CGGAGCGCTG 
GGTGCAGCAG TGAAATCTGC AGAGATCGCC CTACCTCCGC CTCCAGTGCC TCCCAAATCC 
GAAGCCGTCC GGGAAGAGTG CCGGCTCCTG AACGCGCCGC CGGTCCCGCC CCGCAGCGCA 
AAGCCTCAGT CCACCAGCCC CTCGGTCCCT CCTCGCACAG CCAAGCCGGC GCGGCCGCAG 
ACTCGCTCGC CCAGCCCCAC CTTGTCCTAC TATTCTTCAG GGCTGCACGA CATCAGCGTC 
ACTAAAAGTG AGACGAGCCC CCCCGAGAGC GCTCCCGTGT CCTGCTACCC GTGTAACCGA 
GTGAAGGCAG ATTCCGGGGA CCCCAAGTCC CCGTGCGGCA GCCCCCCTTC TGAAGCTCTG 
TCCTCCAGGC TCTCGTGGCC AAACCATTAC TCGGGAGCGT CGGAGACCCA CACCAGGAGC 
GACTTCCTGC TGGATCCGAG CCGCAGCTAC AGTTACCCCC GACAGAAGAC GCCAGGCACG 
CCCAAGAGAA ACTGCCCGGC CGCCTTCGAG CTGGACGGCC GCGAGCTGGC CACCAGCCCG 
CCTGTTCCGG GCCCCGCGGA GCCCGCGGGC TGTCCCAAGT CGGCCAGCTA CTGCCTGGAG 
CGCTCGGGTG ACAGGAGCCT GGCAGGCGGG CCGGCCAAGC AGAGCAGCTC CTGCCCCGCC 
TTGCCCCCCC GGGCCCCGAG GCCGCTGGAA GAGAAAGCGG CCCCGGACAC CCCGCCTCTG 
CCCCTGCAAA TCGACGGTGC TGAGGAAGCC CCCAAGTCCG GGTCGCCGGA CCTGTCCGAG 
GACGCGTACT TCATCCAGAA GGGCGCGCAG GACCTCCTCT CCACGCCCTA CCCCTTCTCG 
TCGCCAATCC ACCTGCAGCT GGCCCCCCGC TCCTGCGGCG ACGGGTCCCC GTGGCAGCCG 
CCCGCCGACC TGTCGGGACT CTCGGTCGAA GAGGTGTCCA AGTCCCTGCG GTTCATCGGG 
CTGTCGGAGG ACGTCATCGT GTTCTTCGTG ACCGAGAAGA TCGATGGCAA TCTGCTCGTT 
CAGCTCACAG AAGAAATCCT CTCGGAGGAT TTCAAACTGA GCAAACTGCA GGTGAAGAAA 
ATTCTGCAGT TCATTAATGG CTGGAGACCC AAAATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005224117.1
CDS	455..3073
Translation	MDPAPSLGCSLKDVKWSSVAVPLDLLVSTYRLPQIARLDSGECVEGLRDNDYLLIHSCRQWTTITAHSLEEGHYVIGPKIEIPVHYAGQFKLLEQDRDIKEPVQYFNSVEEVAKAFPERVYVMEEITFNVKVASGECNEDTEVYNITLCTGDELTLMGQAEILYAKTFKEKSRLNTIFKKIGKLNSISKLGKGKMPCLICMNHRTNESISLPFQCKGRFSTRSPLELQMQEGEHTIRNIVEKTRLPVNVTVPSPPPRNPYDLHFIREGHRYKFVNIQTKTVVVCCVLRNNKILPMHFPLHLTVPKFSLPEHLVKGEGWPETLVHHWLGICQEQFDIDEYSRAVRDVKTDWNEDCKSPKKGRCAGHNHVPNSLSYARDELTQSFHRLSVCVYGNNLHGNSEVNLHGCRDLGGEWAPFPHDILPYQDSGDSGSDYLFPEAGEESAGMAGKSELPYEELWLEEGKPSHQPLTRSLSEKSRCDQFRGSVRSKCATSPLPVPGALGAAVKSAEIALPPPPVPPKSEAVREECRLLNAPPVPPRSAKPQSTSPSVPPRTAKPARPQTRSPSPTLSYYSSGLHDISVTKSETSPPESAPVSCYPCNRVKADSGDPKSPCGSPPSEALSSRLSWPNHYSGASETHTRSDFLLDPSRSYSYPRQKTPGTPKRNCPAAFELDGRELATSPPVPGPAEPAGCPKSASYCLERSGDRSLAGGPAKQSSSCPALPPRAPRPLEEKAAPDTPPLPLQIDGAEEAPKSGSPDLSEDAYFIQKGAQDLLSTPYPFSSPIHLQLAPRSCGDGSPWQPPADLSGLSVEEVSKSLRFIGLSEDVIVFFVTEKIDGNLLVQLTEEILSEDFKLSKLQVKKILQFINGWRPKI

Target ORF information:

RefSeq Version	XM_005224060.4
Organism	Bos taurus(cattle)
Definition	Bos taurus GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005224060.4

ORF Insert Sequence:

ATGGACCCGG CGCCCTCGCT GGGCTGCAGC CTCAAGGATG TGAAGTGGAG CTCGGTGGCC 
GTTCCGCTCG ACCTGCTGGT CAGCACTTAC CGACTGCCCC AGATTGCGCG GCTGGACAGC 
GGAGAGTGCG TAGAAGGGCT GCGGGACAAT GACTACCTTC TGATCCACTC GTGTCGTCAG 
TGGACCACCA TCACCGCCCA CAGCTTGGAG GAGGGGCACT ATGTCATTGG GCCAAAGATA 
GAGATTCCGG TACATTATGC AGGGCAATTC AAGTTGCTGG AACAAGACCG AGATATAAAG 
GAGCCAGTGC AATATTTCAA CAGCGTGGAG GAGGTGGCCA AAGCATTTCC TGAACGCGTG 
TACGTCATGG AGGAAATAAC ATTCAACGTG AAGGTTGCTT CGGGTGAATG CAATGAAGAC 
ACCGAAGTTT ACAACATCAC CCTGTGTACG GGAGATGAAC TCACTCTAAT GGGCCAGGCA 
GAAATCCTTT ATGCAAAGAC TTTCAAGGAG AAGTCGCGAC TCAACACCAT CTTCAAAAAG 
ATTGGGAAAC TCAACTCCAT CAGCAAGCTG GGAAAAGGCA AGATGCCCTG CCTCATTTGC 
ATGAATCACC GGACCAACGA GAGCATCAGC CTCCCGTTCC AGTGCAAGGG CAGGTTTAGC 
ACCCGGAGCC CCCTGGAGCT TCAGATGCAG GAGGGCGAGC ATACCATCCG CAACATCGTG 
GAGAAAACCA GGCTCCCCGT GAACGTGACG GTGCCCAGCC CACCGCCCAG AAACCCGTAT 
GACCTCCACT TCATCCGCGA GGGGCACCGC TACAAGTTTG TGAACATCCA GACCAAGACA 
GTGGTGGTCT GCTGTGTGCT GCGGAACAAC AAGATCCTCC CTATGCACTT CCCTTTGCAC 
TTGACCGTCC CCAAGTTTAG CCTCCCGGAA CACCTGGTCA AGGGAGAGGG ATGGCCTGAA 
ACCCTGGTCC ATCACTGGCT GGGTATCTGC CAAGAACAGT TCGACATCGA TGAGTATTCA 
CGGGCAGTCC GCGACGTGAA AACCGACTGG AACGAGGACT GTAAGAGCCC CAAGAAAGGC 
CGGTGTGCCG GCCATAACCA CGTGCCCAAT TCCCTTAGCT ACGCTCGCGA TGAGCTCACC 
CAGTCCTTCC ACCGGCTCTC GGTCTGTGTG TATGGCAACA ATCTCCATGG CAACAGCGAG 
GTGAACCTCC ACGGCTGCAG GGACCTGGGA GGAGAGTGGG CCCCCTTTCC TCACGACATC 
CTACCCTATC AGGACTCGGG GGACAGCGGG AGCGACTACC TTTTCCCAGA AGCTGGTGAA 
GAATCTGCAG GCATGGCAGG GAAGTCAGAA CTTCCTTATG AAGAGCTGTG GCTGGAGGAA 
GGCAAGCCCA GCCATCAGCC TCTCACTCGC TCCCTGAGCG AGAAGAGCAG ATGCGACCAG 
TTTCGAGGTT CTGTCCGGTC CAAGTGTGCA ACCTCTCCCC TTCCTGTGCC CGGAGCGCTG 
GGTGCAGCAG TGAAATCTGC AGAGATCGCC CTACCTCCGC CTCCAGTGCC TCCCAAATCC 
GAAGCCGTCC GGGAAGAGTG CCGGCTCCTG AACGCGCCGC CGGTCCCGCC CCGCAGCGCA 
AAGCCTCAGT CCACCAGCCC CTCGGTCCCT CCTCGCACAG CCAAGCCGGC GCGGCCGCAG 
ACTCGCTCGC CCAGCCCCAC CTTGTCCTAC TATTCTTCAG GGCTGCACGA CATCAGCGTC 
ACTAAAAGTG AGACGAGCCC CCCCGAGAGC GCTCCCGTGT CCTGCTACCC GTGTAACCGA 
GTGAAGGCAG ATTCCGGGGA CCCCAAGTCC CCGTGCGGCA GCCCCCCTTC TGAAGCTCTG 
TCCTCCAGGC TCTCGTGGCC AAACCATTAC TCGGGAGCGT CGGAGACCCA CACCAGGAGC 
GACTTCCTGC TGGATCCGAG CCGCAGCTAC AGTTACCCCC GACAGAAGAC GCCAGGCACG 
CCCAAGAGAA ACTGCCCGGC CGCCTTCGAG CTGGACGGCC GCGAGCTGGC CACCAGCCCG 
CCTGTTCCGG GCCCCGCGGA GCCCGCGGGC TGTCCCAAGT CGGCCAGCTA CTGCCTGGAG 
CGCTCGGGTG ACAGGAGCCT GGCAGGCGGG CCGGCCAAGC AGAGCAGCTC CTGCCCCGCC 
TTGCCCCCCC GGGCCCCGAG GCCGCTGGAA GAGAAAGCGG CCCCGGACAC CCCGCCTCTG 
CCCCTGCAAA TCGACGGTGC TGAGGAAGCC CCCAAGTCCG GGTCGCCGGA CCTGTCCGAG 
GACGCGTACT TCATCCAGAA GGGCGCGCAG GACCTCCTCT CCACGCCCTA CCCCTTCTCG 
TCGCCAATCC ACCTGCAGCT GGCCCCCCGC TCCTGCGGCG ACGGGTCCCC GTGGCAGCCG 
CCCGCCGACC TGTCGGGACT CTCGGTCGAA GAGGTGTCCA AGTCCCTGCG GTTCATCGGG 
CTGTCGGAGG ACGTCATCGT GTTCTTCGTG ACCGAGAAGA TCGATGGCAA TCTGCTCGTT 
CAGCTCACAG AAGAAATCCT CTCGGAGGAT TTCAAACTGA GCAAACTGCA GGTGAAGAAA 
ATTCTGCAGT TCATTAATGG CTGGAGACCC AAAATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAREM1 ( XM_015469094.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015324580.1 Bos taurus GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa32452
Clone ID Related Accession (Same CDS sequence)	XM_005224060.4 , XM_015469094.1 , XM_005224060.3
Accession Version	XM_015469094.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2619bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	GRB2-associated and regulator of MAPK protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646632.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCGG CGCCCTCGCT GGGCTGCAGC CTCAAGGATG TGAAGTGGAG CTCGGTGGCC 
GTTCCGCTCG ACCTGCTGGT CAGCACTTAC CGACTGCCCC AGATTGCGCG GCTGGACAGC 
GGAGAGTGCG TAGAAGGGCT GCGGGACAAT GACTACCTTC TGATCCACTC GTGTCGTCAG 
TGGACCACCA TCACCGCCCA CAGCTTGGAG GAGGGGCACT ATGTCATTGG GCCAAAGATA 
GAGATTCCGG TACATTATGC AGGGCAATTC AAGTTGCTGG AACAAGACCG AGATATAAAG 
GAGCCAGTGC AATATTTCAA CAGCGTGGAG GAGGTGGCCA AAGCATTTCC TGAACGCGTG 
TACGTCATGG AGGAAATAAC ATTCAACGTG AAGGTTGCTT CGGGTGAATG CAATGAAGAC 
ACCGAAGTTT ACAACATCAC CCTGTGTACG GGAGATGAAC TCACTCTAAT GGGCCAGGCA 
GAAATCCTTT ATGCAAAGAC TTTCAAGGAG AAGTCGCGAC TCAACACCAT CTTCAAAAAG 
ATTGGGAAAC TCAACTCCAT CAGCAAGCTG GGAAAAGGCA AGATGCCCTG CCTCATTTGC 
ATGAATCACC GGACCAACGA GAGCATCAGC CTCCCGTTCC AGTGCAAGGG CAGGTTTAGC 
ACCCGGAGCC CCCTGGAGCT TCAGATGCAG GAGGGCGAGC ATACCATCCG CAACATCGTG 
GAGAAAACCA GGCTCCCCGT GAACGTGACG GTGCCCAGCC CACCGCCCAG AAACCCGTAT 
GACCTCCACT TCATCCGCGA GGGGCACCGC TACAAGTTTG TGAACATCCA GACCAAGACA 
GTGGTGGTCT GCTGTGTGCT GCGGAACAAC AAGATCCTCC CTATGCACTT CCCTTTGCAC 
TTGACCGTCC CCAAGTTTAG CCTCCCGGAA CACCTGGTCA AGGGAGAGGG ATGGCCTGAA 
ACCCTGGTCC ATCACTGGCT GGGTATCTGC CAAGAACAGT TCGACATCGA TGAGTATTCA 
CGGGCAGTCC GCGACGTGAA AACCGACTGG AACGAGGACT GTAAGAGCCC CAAGAAAGGC 
CGGTGTGCCG GCCATAACCA CGTGCCCAAT TCCCTTAGCT ACGCTCGCGA TGAGCTCACC 
CAGTCCTTCC ACCGGCTCTC GGTCTGTGTG TATGGCAACA ATCTCCATGG CAACAGCGAG 
GTGAACCTCC ACGGCTGCAG GGACCTGGGA GGAGAGTGGG CCCCCTTTCC TCACGACATC 
CTACCCTATC AGGACTCGGG GGACAGCGGG AGCGACTACC TTTTCCCAGA AGCTGGTGAA 
GAATCTGCAG GCATGGCAGG GAAGTCAGAA CTTCCTTATG AAGAGCTGTG GCTGGAGGAA 
GGCAAGCCCA GCCATCAGCC TCTCACTCGC TCCCTGAGCG AGAAGAGCAG ATGCGACCAG 
TTTCGAGGTT CTGTCCGGTC CAAGTGTGCA ACCTCTCCCC TTCCTGTGCC CGGAGCGCTG 
GGTGCAGCAG TGAAATCTGC AGAGATCGCC CTACCTCCGC CTCCAGTGCC TCCCAAATCC 
GAAGCCGTCC GGGAAGAGTG CCGGCTCCTG AACGCGCCGC CGGTCCCGCC CCGCAGCGCA 
AAGCCTCAGT CCACCAGCCC CTCGGTCCCT CCTCGCACAG CCAAGCCGGC GCGGCCGCAG 
ACTCGCTCGC CCAGCCCCAC CTTGTCCTAC TATTCTTCAG GGCTGCACGA CATCAGCGTC 
ACTAAAAGTG AGACGAGCCC CCCCGAGAGC GCTCCCGTGT CCTGCTACCC GTGTAACCGA 
GTGAAGGCAG ATTCCGGGGA CCCCAAGTCC CCGTGCGGCA GCCCCCCTTC TGAAGCTCTG 
TCCTCCAGGC TCTCGTGGCC AAACCATTAC TCGGGAGCGT CGGAGACCCA CACCAGGAGC 
GACTTCCTGC TGGATCCGAG CCGCAGCTAC AGTTACCCCC GACAGAAGAC GCCAGGCACG 
CCCAAGAGAA ACTGCCCGGC CGCCTTCGAG CTGGACGGCC GCGAGCTGGC CACCAGCCCG 
CCTGTTCCGG GCCCCGCGGA GCCCGCGGGC TGTCCCAAGT CGGCCAGCTA CTGCCTGGAG 
CGCTCGGGTG ACAGGAGCCT GGCAGGCGGG CCGGCCAAGC AGAGCAGCTC CTGCCCCGCC 
TTGCCCCCCC GGGCCCCGAG GCCGCTGGAA GAGAAAGCGG CCCCGGACAC CCCGCCTCTG 
CCCCTGCAAA TCGACGGTGC TGAGGAAGCC CCCAAGTCCG GGTCGCCGGA CCTGTCCGAG 
GACGCGTACT TCATCCAGAA GGGCGCGCAG GACCTCCTCT CCACGCCCTA CCCCTTCTCG 
TCGCCAATCC ACCTGCAGCT GGCCCCCCGC TCCTGCGGCG ACGGGTCCCC GTGGCAGCCG 
CCCGCCGACC TGTCGGGACT CTCGGTCGAA GAGGTGTCCA AGTCCCTGCG GTTCATCGGG 
CTGTCGGAGG ACGTCATCGT GTTCTTCGTG ACCGAGAAGA TCGATGGCAA TCTGCTCGTT 
CAGCTCACAG AAGAAATCCT CTCGGAGGAT TTCAAACTGA GCAAACTGCA GGTGAAGAAA 
ATTCTGCAGT TCATTAATGG CTGGAGACCC AAAATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015324580.1
CDS	71..2689
Translation	MDPAPSLGCSLKDVKWSSVAVPLDLLVSTYRLPQIARLDSGECVEGLRDNDYLLIHSCRQWTTITAHSLEEGHYVIGPKIEIPVHYAGQFKLLEQDRDIKEPVQYFNSVEEVAKAFPERVYVMEEITFNVKVASGECNEDTEVYNITLCTGDELTLMGQAEILYAKTFKEKSRLNTIFKKIGKLNSISKLGKGKMPCLICMNHRTNESISLPFQCKGRFSTRSPLELQMQEGEHTIRNIVEKTRLPVNVTVPSPPPRNPYDLHFIREGHRYKFVNIQTKTVVVCCVLRNNKILPMHFPLHLTVPKFSLPEHLVKGEGWPETLVHHWLGICQEQFDIDEYSRAVRDVKTDWNEDCKSPKKGRCAGHNHVPNSLSYARDELTQSFHRLSVCVYGNNLHGNSEVNLHGCRDLGGEWAPFPHDILPYQDSGDSGSDYLFPEAGEESAGMAGKSELPYEELWLEEGKPSHQPLTRSLSEKSRCDQFRGSVRSKCATSPLPVPGALGAAVKSAEIALPPPPVPPKSEAVREECRLLNAPPVPPRSAKPQSTSPSVPPRTAKPARPQTRSPSPTLSYYSSGLHDISVTKSETSPPESAPVSCYPCNRVKADSGDPKSPCGSPPSEALSSRLSWPNHYSGASETHTRSDFLLDPSRSYSYPRQKTPGTPKRNCPAAFELDGRELATSPPVPGPAEPAGCPKSASYCLERSGDRSLAGGPAKQSSSCPALPPRAPRPLEEKAAPDTPPLPLQIDGAEEAPKSGSPDLSEDAYFIQKGAQDLLSTPYPFSSPIHLQLAPRSCGDGSPWQPPADLSGLSVEEVSKSLRFIGLSEDVIVFFVTEKIDGNLLVQLTEEILSEDFKLSKLQVKKILQFINGWRPKI

Target ORF information:

RefSeq Version	XM_015469094.1
Organism	Bos taurus(cattle)
Definition	Bos taurus GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015469094.1

ORF Insert Sequence:

ATGGACCCGG CGCCCTCGCT GGGCTGCAGC CTCAAGGATG TGAAGTGGAG CTCGGTGGCC 
GTTCCGCTCG ACCTGCTGGT CAGCACTTAC CGACTGCCCC AGATTGCGCG GCTGGACAGC 
GGAGAGTGCG TAGAAGGGCT GCGGGACAAT GACTACCTTC TGATCCACTC GTGTCGTCAG 
TGGACCACCA TCACCGCCCA CAGCTTGGAG GAGGGGCACT ATGTCATTGG GCCAAAGATA 
GAGATTCCGG TACATTATGC AGGGCAATTC AAGTTGCTGG AACAAGACCG AGATATAAAG 
GAGCCAGTGC AATATTTCAA CAGCGTGGAG GAGGTGGCCA AAGCATTTCC TGAACGCGTG 
TACGTCATGG AGGAAATAAC ATTCAACGTG AAGGTTGCTT CGGGTGAATG CAATGAAGAC 
ACCGAAGTTT ACAACATCAC CCTGTGTACG GGAGATGAAC TCACTCTAAT GGGCCAGGCA 
GAAATCCTTT ATGCAAAGAC TTTCAAGGAG AAGTCGCGAC TCAACACCAT CTTCAAAAAG 
ATTGGGAAAC TCAACTCCAT CAGCAAGCTG GGAAAAGGCA AGATGCCCTG CCTCATTTGC 
ATGAATCACC GGACCAACGA GAGCATCAGC CTCCCGTTCC AGTGCAAGGG CAGGTTTAGC 
ACCCGGAGCC CCCTGGAGCT TCAGATGCAG GAGGGCGAGC ATACCATCCG CAACATCGTG 
GAGAAAACCA GGCTCCCCGT GAACGTGACG GTGCCCAGCC CACCGCCCAG AAACCCGTAT 
GACCTCCACT TCATCCGCGA GGGGCACCGC TACAAGTTTG TGAACATCCA GACCAAGACA 
GTGGTGGTCT GCTGTGTGCT GCGGAACAAC AAGATCCTCC CTATGCACTT CCCTTTGCAC 
TTGACCGTCC CCAAGTTTAG CCTCCCGGAA CACCTGGTCA AGGGAGAGGG ATGGCCTGAA 
ACCCTGGTCC ATCACTGGCT GGGTATCTGC CAAGAACAGT TCGACATCGA TGAGTATTCA 
CGGGCAGTCC GCGACGTGAA AACCGACTGG AACGAGGACT GTAAGAGCCC CAAGAAAGGC 
CGGTGTGCCG GCCATAACCA CGTGCCCAAT TCCCTTAGCT ACGCTCGCGA TGAGCTCACC 
CAGTCCTTCC ACCGGCTCTC GGTCTGTGTG TATGGCAACA ATCTCCATGG CAACAGCGAG 
GTGAACCTCC ACGGCTGCAG GGACCTGGGA GGAGAGTGGG CCCCCTTTCC TCACGACATC 
CTACCCTATC AGGACTCGGG GGACAGCGGG AGCGACTACC TTTTCCCAGA AGCTGGTGAA 
GAATCTGCAG GCATGGCAGG GAAGTCAGAA CTTCCTTATG AAGAGCTGTG GCTGGAGGAA 
GGCAAGCCCA GCCATCAGCC TCTCACTCGC TCCCTGAGCG AGAAGAGCAG ATGCGACCAG 
TTTCGAGGTT CTGTCCGGTC CAAGTGTGCA ACCTCTCCCC TTCCTGTGCC CGGAGCGCTG 
GGTGCAGCAG TGAAATCTGC AGAGATCGCC CTACCTCCGC CTCCAGTGCC TCCCAAATCC 
GAAGCCGTCC GGGAAGAGTG CCGGCTCCTG AACGCGCCGC CGGTCCCGCC CCGCAGCGCA 
AAGCCTCAGT CCACCAGCCC CTCGGTCCCT CCTCGCACAG CCAAGCCGGC GCGGCCGCAG 
ACTCGCTCGC CCAGCCCCAC CTTGTCCTAC TATTCTTCAG GGCTGCACGA CATCAGCGTC 
ACTAAAAGTG AGACGAGCCC CCCCGAGAGC GCTCCCGTGT CCTGCTACCC GTGTAACCGA 
GTGAAGGCAG ATTCCGGGGA CCCCAAGTCC CCGTGCGGCA GCCCCCCTTC TGAAGCTCTG 
TCCTCCAGGC TCTCGTGGCC AAACCATTAC TCGGGAGCGT CGGAGACCCA CACCAGGAGC 
GACTTCCTGC TGGATCCGAG CCGCAGCTAC AGTTACCCCC GACAGAAGAC GCCAGGCACG 
CCCAAGAGAA ACTGCCCGGC CGCCTTCGAG CTGGACGGCC GCGAGCTGGC CACCAGCCCG 
CCTGTTCCGG GCCCCGCGGA GCCCGCGGGC TGTCCCAAGT CGGCCAGCTA CTGCCTGGAG 
CGCTCGGGTG ACAGGAGCCT GGCAGGCGGG CCGGCCAAGC AGAGCAGCTC CTGCCCCGCC 
TTGCCCCCCC GGGCCCCGAG GCCGCTGGAA GAGAAAGCGG CCCCGGACAC CCCGCCTCTG 
CCCCTGCAAA TCGACGGTGC TGAGGAAGCC CCCAAGTCCG GGTCGCCGGA CCTGTCCGAG 
GACGCGTACT TCATCCAGAA GGGCGCGCAG GACCTCCTCT CCACGCCCTA CCCCTTCTCG 
TCGCCAATCC ACCTGCAGCT GGCCCCCCGC TCCTGCGGCG ACGGGTCCCC GTGGCAGCCG 
CCCGCCGACC TGTCGGGACT CTCGGTCGAA GAGGTGTCCA AGTCCCTGCG GTTCATCGGG 
CTGTCGGAGG ACGTCATCGT GTTCTTCGTG ACCGAGAAGA TCGATGGCAA TCTGCTCGTT 
CAGCTCACAG AAGAAATCCT CTCGGAGGAT TTCAAACTGA GCAAACTGCA GGTGAAGAAA 
ATTCTGCAGT TCATTAATGG CTGGAGACCC AAAATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAREM1 ( XM_005224060.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005224117.1 Bos taurus GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa32452
Clone ID Related Accession (Same CDS sequence)	XM_005224060.4 , XM_015469094.1 , XM_005224060.3
Accession Version	XM_005224060.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2619bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	GRB2-associated and regulator of MAPK protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104566.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005224060.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCGG CGCCCTCGCT GGGCTGCAGC CTCAAGGATG TGAAGTGGAG CTCGGTGGCC 
GTTCCGCTCG ACCTGCTGGT CAGCACTTAC CGACTGCCCC AGATTGCGCG GCTGGACAGC 
GGAGAGTGCG TAGAAGGGCT GCGGGACAAT GACTACCTTC TGATCCACTC GTGTCGTCAG 
TGGACCACCA TCACCGCCCA CAGCTTGGAG GAGGGGCACT ATGTCATTGG GCCAAAGATA 
GAGATTCCGG TACATTATGC AGGGCAATTC AAGTTGCTGG AACAAGACCG AGATATAAAG 
GAGCCAGTGC AATATTTCAA CAGCGTGGAG GAGGTGGCCA AAGCATTTCC TGAACGCGTG 
TACGTCATGG AGGAAATAAC ATTCAACGTG AAGGTTGCTT CGGGTGAATG CAATGAAGAC 
ACCGAAGTTT ACAACATCAC CCTGTGTACG GGAGATGAAC TCACTCTAAT GGGCCAGGCA 
GAAATCCTTT ATGCAAAGAC TTTCAAGGAG AAGTCGCGAC TCAACACCAT CTTCAAAAAG 
ATTGGGAAAC TCAACTCCAT CAGCAAGCTG GGAAAAGGCA AGATGCCCTG CCTCATTTGC 
ATGAATCACC GGACCAACGA GAGCATCAGC CTCCCGTTCC AGTGCAAGGG CAGGTTTAGC 
ACCCGGAGCC CCCTGGAGCT TCAGATGCAG GAGGGCGAGC ATACCATCCG CAACATCGTG 
GAGAAAACCA GGCTCCCCGT GAACGTGACG GTGCCCAGCC CACCGCCCAG AAACCCGTAT 
GACCTCCACT TCATCCGCGA GGGGCACCGC TACAAGTTTG TGAACATCCA GACCAAGACA 
GTGGTGGTCT GCTGTGTGCT GCGGAACAAC AAGATCCTCC CTATGCACTT CCCTTTGCAC 
TTGACCGTCC CCAAGTTTAG CCTCCCGGAA CACCTGGTCA AGGGAGAGGG ATGGCCTGAA 
ACCCTGGTCC ATCACTGGCT GGGTATCTGC CAAGAACAGT TCGACATCGA TGAGTATTCA 
CGGGCAGTCC GCGACGTGAA AACCGACTGG AACGAGGACT GTAAGAGCCC CAAGAAAGGC 
CGGTGTGCCG GCCATAACCA CGTGCCCAAT TCCCTTAGCT ACGCTCGCGA TGAGCTCACC 
CAGTCCTTCC ACCGGCTCTC GGTCTGTGTG TATGGCAACA ATCTCCATGG CAACAGCGAG 
GTGAACCTCC ACGGCTGCAG GGACCTGGGA GGAGAGTGGG CCCCCTTTCC TCACGACATC 
CTACCCTATC AGGACTCGGG GGACAGCGGG AGCGACTACC TTTTCCCAGA AGCTGGTGAA 
GAATCTGCAG GCATGGCAGG GAAGTCAGAA CTTCCTTATG AAGAGCTGTG GCTGGAGGAA 
GGCAAGCCCA GCCATCAGCC TCTCACTCGC TCCCTGAGCG AGAAGAGCAG ATGCGACCAG 
TTTCGAGGTT CTGTCCGGTC CAAGTGTGCA ACCTCTCCCC TTCCTGTGCC CGGAGCGCTG 
GGTGCAGCAG TGAAATCTGC AGAGATCGCC CTACCTCCGC CTCCAGTGCC TCCCAAATCC 
GAAGCCGTCC GGGAAGAGTG CCGGCTCCTG AACGCGCCGC CGGTCCCGCC CCGCAGCGCA 
AAGCCTCAGT CCACCAGCCC CTCGGTCCCT CCTCGCACAG CCAAGCCGGC GCGGCCGCAG 
ACTCGCTCGC CCAGCCCCAC CTTGTCCTAC TATTCTTCAG GGCTGCACGA CATCAGCGTC 
ACTAAAAGTG AGACGAGCCC CCCCGAGAGC GCTCCCGTGT CCTGCTACCC GTGTAACCGA 
GTGAAGGCAG ATTCCGGGGA CCCCAAGTCC CCGTGCGGCA GCCCCCCTTC TGAAGCTCTG 
TCCTCCAGGC TCTCGTGGCC AAACCATTAC TCGGGAGCGT CGGAGACCCA CACCAGGAGC 
GACTTCCTGC TGGATCCGAG CCGCAGCTAC AGTTACCCCC GACAGAAGAC GCCAGGCACG 
CCCAAGAGAA ACTGCCCGGC CGCCTTCGAG CTGGACGGCC GCGAGCTGGC CACCAGCCCG 
CCTGTTCCGG GCCCCGCGGA GCCCGCGGGC TGTCCCAAGT CGGCCAGCTA CTGCCTGGAG 
CGCTCGGGTG ACAGGAGCCT GGCAGGCGGG CCGGCCAAGC AGAGCAGCTC CTGCCCCGCC 
TTGCCCCCCC GGGCCCCGAG GCCGCTGGAA GAGAAAGCGG CCCCGGACAC CCCGCCTCTG 
CCCCTGCAAA TCGACGGTGC TGAGGAAGCC CCCAAGTCCG GGTCGCCGGA CCTGTCCGAG 
GACGCGTACT TCATCCAGAA GGGCGCGCAG GACCTCCTCT CCACGCCCTA CCCCTTCTCG 
TCGCCAATCC ACCTGCAGCT GGCCCCCCGC TCCTGCGGCG ACGGGTCCCC GTGGCAGCCG 
CCCGCCGACC TGTCGGGACT CTCGGTCGAA GAGGTGTCCA AGTCCCTGCG GTTCATCGGG 
CTGTCGGAGG ACGTCATCGT GTTCTTCGTG ACCGAGAAGA TCGATGGCAA TCTGCTCGTT 
CAGCTCACAG AAGAAATCCT CTCGGAGGAT TTCAAACTGA GCAAACTGCA GGTGAAGAAA 
ATTCTGCAGT TCATTAATGG CTGGAGACCC AAAATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005224117.1
CDS	71..2689
Translation	MDPAPSLGCSLKDVKWSSVAVPLDLLVSTYRLPQIARLDSGECVEGLRDNDYLLIHSCRQWTTITAHSLEEGHYVIGPKIEIPVHYAGQFKLLEQDRDIKEPVQYFNSVEEVAKAFPERVYVMEEITFNVKVASGECNEDTEVYNITLCTGDELTLMGQAEILYAKTFKEKSRLNTIFKKIGKLNSISKLGKGKMPCLICMNHRTNESISLPFQCKGRFSTRSPLELQMQEGEHTIRNIVEKTRLPVNVTVPSPPPRNPYDLHFIREGHRYKFVNIQTKTVVVCCVLRNNKILPMHFPLHLTVPKFSLPEHLVKGEGWPETLVHHWLGICQEQFDIDEYSRAVRDVKTDWNEDCKSPKKGRCAGHNHVPNSLSYARDELTQSFHRLSVCVYGNNLHGNSEVNLHGCRDLGGEWAPFPHDILPYQDSGDSGSDYLFPEAGEESAGMAGKSELPYEELWLEEGKPSHQPLTRSLSEKSRCDQFRGSVRSKCATSPLPVPGALGAAVKSAEIALPPPPVPPKSEAVREECRLLNAPPVPPRSAKPQSTSPSVPPRTAKPARPQTRSPSPTLSYYSSGLHDISVTKSETSPPESAPVSCYPCNRVKADSGDPKSPCGSPPSEALSSRLSWPNHYSGASETHTRSDFLLDPSRSYSYPRQKTPGTPKRNCPAAFELDGRELATSPPVPGPAEPAGCPKSASYCLERSGDRSLAGGPAKQSSSCPALPPRAPRPLEEKAAPDTPPLPLQIDGAEEAPKSGSPDLSEDAYFIQKGAQDLLSTPYPFSSPIHLQLAPRSCGDGSPWQPPADLSGLSVEEVSKSLRFIGLSEDVIVFFVTEKIDGNLLVQLTEEILSEDFKLSKLQVKKILQFINGWRPKI

Target ORF information:

RefSeq Version	XM_005224060.3
Organism	Bos taurus(cattle)
Definition	Bos taurus GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005224060.3

ORF Insert Sequence:

ATGGACCCGG CGCCCTCGCT GGGCTGCAGC CTCAAGGATG TGAAGTGGAG CTCGGTGGCC 
GTTCCGCTCG ACCTGCTGGT CAGCACTTAC CGACTGCCCC AGATTGCGCG GCTGGACAGC 
GGAGAGTGCG TAGAAGGGCT GCGGGACAAT GACTACCTTC TGATCCACTC GTGTCGTCAG 
TGGACCACCA TCACCGCCCA CAGCTTGGAG GAGGGGCACT ATGTCATTGG GCCAAAGATA 
GAGATTCCGG TACATTATGC AGGGCAATTC AAGTTGCTGG AACAAGACCG AGATATAAAG 
GAGCCAGTGC AATATTTCAA CAGCGTGGAG GAGGTGGCCA AAGCATTTCC TGAACGCGTG 
TACGTCATGG AGGAAATAAC ATTCAACGTG AAGGTTGCTT CGGGTGAATG CAATGAAGAC 
ACCGAAGTTT ACAACATCAC CCTGTGTACG GGAGATGAAC TCACTCTAAT GGGCCAGGCA 
GAAATCCTTT ATGCAAAGAC TTTCAAGGAG AAGTCGCGAC TCAACACCAT CTTCAAAAAG 
ATTGGGAAAC TCAACTCCAT CAGCAAGCTG GGAAAAGGCA AGATGCCCTG CCTCATTTGC 
ATGAATCACC GGACCAACGA GAGCATCAGC CTCCCGTTCC AGTGCAAGGG CAGGTTTAGC 
ACCCGGAGCC CCCTGGAGCT TCAGATGCAG GAGGGCGAGC ATACCATCCG CAACATCGTG 
GAGAAAACCA GGCTCCCCGT GAACGTGACG GTGCCCAGCC CACCGCCCAG AAACCCGTAT 
GACCTCCACT TCATCCGCGA GGGGCACCGC TACAAGTTTG TGAACATCCA GACCAAGACA 
GTGGTGGTCT GCTGTGTGCT GCGGAACAAC AAGATCCTCC CTATGCACTT CCCTTTGCAC 
TTGACCGTCC CCAAGTTTAG CCTCCCGGAA CACCTGGTCA AGGGAGAGGG ATGGCCTGAA 
ACCCTGGTCC ATCACTGGCT GGGTATCTGC CAAGAACAGT TCGACATCGA TGAGTATTCA 
CGGGCAGTCC GCGACGTGAA AACCGACTGG AACGAGGACT GTAAGAGCCC CAAGAAAGGC 
CGGTGTGCCG GCCATAACCA CGTGCCCAAT TCCCTTAGCT ACGCTCGCGA TGAGCTCACC 
CAGTCCTTCC ACCGGCTCTC GGTCTGTGTG TATGGCAACA ATCTCCATGG CAACAGCGAG 
GTGAACCTCC ACGGCTGCAG GGACCTGGGA GGAGAGTGGG CCCCCTTTCC TCACGACATC 
CTACCCTATC AGGACTCGGG GGACAGCGGG AGCGACTACC TTTTCCCAGA AGCTGGTGAA 
GAATCTGCAG GCATGGCAGG GAAGTCAGAA CTTCCTTATG AAGAGCTGTG GCTGGAGGAA 
GGCAAGCCCA GCCATCAGCC TCTCACTCGC TCCCTGAGCG AGAAGAGCAG ATGCGACCAG 
TTTCGAGGTT CTGTCCGGTC CAAGTGTGCA ACCTCTCCCC TTCCTGTGCC CGGAGCGCTG 
GGTGCAGCAG TGAAATCTGC AGAGATCGCC CTACCTCCGC CTCCAGTGCC TCCCAAATCC 
GAAGCCGTCC GGGAAGAGTG CCGGCTCCTG AACGCGCCGC CGGTCCCGCC CCGCAGCGCA 
AAGCCTCAGT CCACCAGCCC CTCGGTCCCT CCTCGCACAG CCAAGCCGGC GCGGCCGCAG 
ACTCGCTCGC CCAGCCCCAC CTTGTCCTAC TATTCTTCAG GGCTGCACGA CATCAGCGTC 
ACTAAAAGTG AGACGAGCCC CCCCGAGAGC GCTCCCGTGT CCTGCTACCC GTGTAACCGA 
GTGAAGGCAG ATTCCGGGGA CCCCAAGTCC CCGTGCGGCA GCCCCCCTTC TGAAGCTCTG 
TCCTCCAGGC TCTCGTGGCC AAACCATTAC TCGGGAGCGT CGGAGACCCA CACCAGGAGC 
GACTTCCTGC TGGATCCGAG CCGCAGCTAC AGTTACCCCC GACAGAAGAC GCCAGGCACG 
CCCAAGAGAA ACTGCCCGGC CGCCTTCGAG CTGGACGGCC GCGAGCTGGC CACCAGCCCG 
CCTGTTCCGG GCCCCGCGGA GCCCGCGGGC TGTCCCAAGT CGGCCAGCTA CTGCCTGGAG 
CGCTCGGGTG ACAGGAGCCT GGCAGGCGGG CCGGCCAAGC AGAGCAGCTC CTGCCCCGCC 
TTGCCCCCCC GGGCCCCGAG GCCGCTGGAA GAGAAAGCGG CCCCGGACAC CCCGCCTCTG 
CCCCTGCAAA TCGACGGTGC TGAGGAAGCC CCCAAGTCCG GGTCGCCGGA CCTGTCCGAG 
GACGCGTACT TCATCCAGAA GGGCGCGCAG GACCTCCTCT CCACGCCCTA CCCCTTCTCG 
TCGCCAATCC ACCTGCAGCT GGCCCCCCGC TCCTGCGGCG ACGGGTCCCC GTGGCAGCCG 
CCCGCCGACC TGTCGGGACT CTCGGTCGAA GAGGTGTCCA AGTCCCTGCG GTTCATCGGG 
CTGTCGGAGG ACGTCATCGT GTTCTTCGTG ACCGAGAAGA TCGATGGCAA TCTGCTCGTT 
CAGCTCACAG AAGAAATCCT CTCGGAGGAT TTCAAACTGA GCAAACTGCA GGTGAAGAAA 
ATTCTGCAGT TCATTAATGG CTGGAGACCC AAAATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAREM1 ( XM_015469095.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015324581.1 Bos taurus GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa32453
Clone ID Related Accession (Same CDS sequence)	XM_015469095.1 , XM_002697684.4 , XM_002697684.5
Accession Version	XM_015469095.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2616bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	GRB2-associated and regulator of MAPK protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646632.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCGG CGCCCTCGCT GGGCTGCAGC CTCAAGGATG TGAAGTGGAG CTCGGTGGCC 
GTTCCGCTCG ACCTGCTGGT CAGCACTTAC CGACTGCCCC AGATTGCGCG GCTGGACAGC 
GGAGAGTGCG TAGAAGGGCT GCGGGACAAT GACTACCTTC TGATCCACTC GTGTCGTCAG 
TGGACCACCA TCACCGCCCA CAGCTTGGAG GAGGGGCACT ATGTCATTGG GCCAAAGATA 
GAGATTCCGG TACATTATGC AGGGCAATTC AAGTTGCTGG AACAAGACCG AGATATAAAG 
GAGCCAGTGC AATATTTCAA CAGCGTGGAG GAGGTGGCCA AAGCATTTCC TGAACGCGTG 
TACGTCATGG AGGAAATAAC ATTCAACGTG AAGGTTGCTT CGGGTGAATG CAATGAAGAC 
ACCGAAGTTT ACAACATCAC CCTGTGTACG GGAGATGAAC TCACTCTAAT GGGCCAGGCA 
GAAATCCTTT ATGCAAAGAC TTTCAAGGAG AAGTCGCGAC TCAACACCAT CTTCAAAAAG 
ATTGGGAAAC TCAACTCCAT CAGCAAGCTG GGAAAAGGCA AGATGCCCTG CCTCATTTGC 
ATGAATCACC GGACCAACGA GAGCATCAGC CTCCCGTTCC AGTGCAAGGG CAGGTTTAGC 
ACCCGGAGCC CCCTGGAGCT TCAGATGCAG GAGGGCGAGC ATACCATCCG CAACATCGTG 
GAGAAAACCA GGCTCCCCGT GAACGTGACG GTGCCCAGCC CACCGCCCAG AAACCCGTAT 
GACCTCCACT TCATCCGCGA GGGGCACCGC TACAAGTTTG TGAACATCCA GACCAAGACA 
GTGGTGGTCT GCTGTGTGCT GCGGAACAAC AAGATCCTCC CTATGCACTT CCCTTTGCAC 
TTGACCGTCC CCAAGTTTAG CCTCCCGGAA CACCTGGTCA AGGGAGAGGG ATGGCCTGAA 
ACCCTGGTCC ATCACTGGCT GGGTATCTGC CAAGAACAGT TCGACATCGA TGAGTATTCA 
CGGGCAGTCC GCGACGTGAA AACCGACTGG AACGAGGACT GTAAGAGCCC CAAGAAAGGC 
CGGTGTGCCG GCCATAACCA CGTGCCCAAT TCCCTTAGCT ACGCTCGCGA TGAGCTCACC 
CAGTCCTTCC ACCGGCTCTC GGTCTGTGTG TATGGCAACA ATCTCCATGG CAACAGCGAG 
GTGAACCTCC ACGGCTGCAG GGACCTGGGA GGAGAGTGGG CCCCCTTTCC TCACGACATC 
CTACCCTATC AGGACTCGGG GGACAGCGGG AGCGACTACC TTTTCCCAGA AGCTGGTGAA 
GAATCTGCAG GCATGGCAGG GAAGTCAGAA CTTCCTTATG AAGAGCTGTG GCTGGAGGAA 
GGCAAGCCCA GCCATCAGCC TCTCACTCGC TCCCTGAGCG AGAAGAGCAG ATGCGACCAG 
TTTCGAGGTT CTGTCCGGTC CAAGTGTGCA ACCTCTCCCC TTCCTGTGCC CGGAGCGCTG 
GGTGCAGCAG TGAAATCTGC AGAGATCGCC CTACCTCCGC CTCCAGTGCC TCCCAAATCC 
GAAGCCGTCC GGGAAGAGTG CCGGCTCCTG AACGCGCCGC CGGTCCCGCC CCGCAGCGCA 
AAGCCTCAGT CCACCAGCCC CTCGGTCCCT CCTCGCACAG CCAAGCCGGC GCGGCCGCAG 
ACTCGCTCGC CCAGCCCCAC CTTGTCCTAC TATTCTTCAG GGCTGCACGA CATCGTCACT 
AAAAGTGAGA CGAGCCCCCC CGAGAGCGCT CCCGTGTCCT GCTACCCGTG TAACCGAGTG 
AAGGCAGATT CCGGGGACCC CAAGTCCCCG TGCGGCAGCC CCCCTTCTGA AGCTCTGTCC 
TCCAGGCTCT CGTGGCCAAA CCATTACTCG GGAGCGTCGG AGACCCACAC CAGGAGCGAC 
TTCCTGCTGG ATCCGAGCCG CAGCTACAGT TACCCCCGAC AGAAGACGCC AGGCACGCCC 
AAGAGAAACT GCCCGGCCGC CTTCGAGCTG GACGGCCGCG AGCTGGCCAC CAGCCCGCCT 
GTTCCGGGCC CCGCGGAGCC CGCGGGCTGT CCCAAGTCGG CCAGCTACTG CCTGGAGCGC 
TCGGGTGACA GGAGCCTGGC AGGCGGGCCG GCCAAGCAGA GCAGCTCCTG CCCCGCCTTG 
CCCCCCCGGG CCCCGAGGCC GCTGGAAGAG AAAGCGGCCC CGGACACCCC GCCTCTGCCC 
CTGCAAATCG ACGGTGCTGA GGAAGCCCCC AAGTCCGGGT CGCCGGACCT GTCCGAGGAC 
GCGTACTTCA TCCAGAAGGG CGCGCAGGAC CTCCTCTCCA CGCCCTACCC CTTCTCGTCG 
CCAATCCACC TGCAGCTGGC CCCCCGCTCC TGCGGCGACG GGTCCCCGTG GCAGCCGCCC 
GCCGACCTGT CGGGACTCTC GGTCGAAGAG GTGTCCAAGT CCCTGCGGTT CATCGGGCTG 
TCGGAGGACG TCATCGTGTT CTTCGTGACC GAGAAGATCG ATGGCAATCT GCTCGTTCAG 
CTCACAGAAG AAATCCTCTC GGAGGATTTC AAACTGAGCA AACTGCAGGT GAAGAAAATT 
CTGCAGTTCA TTAATGGCTG GAGACCCAAA ATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015324581.1
CDS	71..2686
Translation	MDPAPSLGCSLKDVKWSSVAVPLDLLVSTYRLPQIARLDSGECVEGLRDNDYLLIHSCRQWTTITAHSLEEGHYVIGPKIEIPVHYAGQFKLLEQDRDIKEPVQYFNSVEEVAKAFPERVYVMEEITFNVKVASGECNEDTEVYNITLCTGDELTLMGQAEILYAKTFKEKSRLNTIFKKIGKLNSISKLGKGKMPCLICMNHRTNESISLPFQCKGRFSTRSPLELQMQEGEHTIRNIVEKTRLPVNVTVPSPPPRNPYDLHFIREGHRYKFVNIQTKTVVVCCVLRNNKILPMHFPLHLTVPKFSLPEHLVKGEGWPETLVHHWLGICQEQFDIDEYSRAVRDVKTDWNEDCKSPKKGRCAGHNHVPNSLSYARDELTQSFHRLSVCVYGNNLHGNSEVNLHGCRDLGGEWAPFPHDILPYQDSGDSGSDYLFPEAGEESAGMAGKSELPYEELWLEEGKPSHQPLTRSLSEKSRCDQFRGSVRSKCATSPLPVPGALGAAVKSAEIALPPPPVPPKSEAVREECRLLNAPPVPPRSAKPQSTSPSVPPRTAKPARPQTRSPSPTLSYYSSGLHDIVTKSETSPPESAPVSCYPCNRVKADSGDPKSPCGSPPSEALSSRLSWPNHYSGASETHTRSDFLLDPSRSYSYPRQKTPGTPKRNCPAAFELDGRELATSPPVPGPAEPAGCPKSASYCLERSGDRSLAGGPAKQSSSCPALPPRAPRPLEEKAAPDTPPLPLQIDGAEEAPKSGSPDLSEDAYFIQKGAQDLLSTPYPFSSPIHLQLAPRSCGDGSPWQPPADLSGLSVEEVSKSLRFIGLSEDVIVFFVTEKIDGNLLVQLTEEILSEDFKLSKLQVKKILQFINGWRPKI

Target ORF information:

RefSeq Version	XM_015469095.1
Organism	Bos taurus(cattle)
Definition	Bos taurus GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015469095.1

ORF Insert Sequence:

ATGGACCCGG CGCCCTCGCT GGGCTGCAGC CTCAAGGATG TGAAGTGGAG CTCGGTGGCC 
GTTCCGCTCG ACCTGCTGGT CAGCACTTAC CGACTGCCCC AGATTGCGCG GCTGGACAGC 
GGAGAGTGCG TAGAAGGGCT GCGGGACAAT GACTACCTTC TGATCCACTC GTGTCGTCAG 
TGGACCACCA TCACCGCCCA CAGCTTGGAG GAGGGGCACT ATGTCATTGG GCCAAAGATA 
GAGATTCCGG TACATTATGC AGGGCAATTC AAGTTGCTGG AACAAGACCG AGATATAAAG 
GAGCCAGTGC AATATTTCAA CAGCGTGGAG GAGGTGGCCA AAGCATTTCC TGAACGCGTG 
TACGTCATGG AGGAAATAAC ATTCAACGTG AAGGTTGCTT CGGGTGAATG CAATGAAGAC 
ACCGAAGTTT ACAACATCAC CCTGTGTACG GGAGATGAAC TCACTCTAAT GGGCCAGGCA 
GAAATCCTTT ATGCAAAGAC TTTCAAGGAG AAGTCGCGAC TCAACACCAT CTTCAAAAAG 
ATTGGGAAAC TCAACTCCAT CAGCAAGCTG GGAAAAGGCA AGATGCCCTG CCTCATTTGC 
ATGAATCACC GGACCAACGA GAGCATCAGC CTCCCGTTCC AGTGCAAGGG CAGGTTTAGC 
ACCCGGAGCC CCCTGGAGCT TCAGATGCAG GAGGGCGAGC ATACCATCCG CAACATCGTG 
GAGAAAACCA GGCTCCCCGT GAACGTGACG GTGCCCAGCC CACCGCCCAG AAACCCGTAT 
GACCTCCACT TCATCCGCGA GGGGCACCGC TACAAGTTTG TGAACATCCA GACCAAGACA 
GTGGTGGTCT GCTGTGTGCT GCGGAACAAC AAGATCCTCC CTATGCACTT CCCTTTGCAC 
TTGACCGTCC CCAAGTTTAG CCTCCCGGAA CACCTGGTCA AGGGAGAGGG ATGGCCTGAA 
ACCCTGGTCC ATCACTGGCT GGGTATCTGC CAAGAACAGT TCGACATCGA TGAGTATTCA 
CGGGCAGTCC GCGACGTGAA AACCGACTGG AACGAGGACT GTAAGAGCCC CAAGAAAGGC 
CGGTGTGCCG GCCATAACCA CGTGCCCAAT TCCCTTAGCT ACGCTCGCGA TGAGCTCACC 
CAGTCCTTCC ACCGGCTCTC GGTCTGTGTG TATGGCAACA ATCTCCATGG CAACAGCGAG 
GTGAACCTCC ACGGCTGCAG GGACCTGGGA GGAGAGTGGG CCCCCTTTCC TCACGACATC 
CTACCCTATC AGGACTCGGG GGACAGCGGG AGCGACTACC TTTTCCCAGA AGCTGGTGAA 
GAATCTGCAG GCATGGCAGG GAAGTCAGAA CTTCCTTATG AAGAGCTGTG GCTGGAGGAA 
GGCAAGCCCA GCCATCAGCC TCTCACTCGC TCCCTGAGCG AGAAGAGCAG ATGCGACCAG 
TTTCGAGGTT CTGTCCGGTC CAAGTGTGCA ACCTCTCCCC TTCCTGTGCC CGGAGCGCTG 
GGTGCAGCAG TGAAATCTGC AGAGATCGCC CTACCTCCGC CTCCAGTGCC TCCCAAATCC 
GAAGCCGTCC GGGAAGAGTG CCGGCTCCTG AACGCGCCGC CGGTCCCGCC CCGCAGCGCA 
AAGCCTCAGT CCACCAGCCC CTCGGTCCCT CCTCGCACAG CCAAGCCGGC GCGGCCGCAG 
ACTCGCTCGC CCAGCCCCAC CTTGTCCTAC TATTCTTCAG GGCTGCACGA CATCGTCACT 
AAAAGTGAGA CGAGCCCCCC CGAGAGCGCT CCCGTGTCCT GCTACCCGTG TAACCGAGTG 
AAGGCAGATT CCGGGGACCC CAAGTCCCCG TGCGGCAGCC CCCCTTCTGA AGCTCTGTCC 
TCCAGGCTCT CGTGGCCAAA CCATTACTCG GGAGCGTCGG AGACCCACAC CAGGAGCGAC 
TTCCTGCTGG ATCCGAGCCG CAGCTACAGT TACCCCCGAC AGAAGACGCC AGGCACGCCC 
AAGAGAAACT GCCCGGCCGC CTTCGAGCTG GACGGCCGCG AGCTGGCCAC CAGCCCGCCT 
GTTCCGGGCC CCGCGGAGCC CGCGGGCTGT CCCAAGTCGG CCAGCTACTG CCTGGAGCGC 
TCGGGTGACA GGAGCCTGGC AGGCGGGCCG GCCAAGCAGA GCAGCTCCTG CCCCGCCTTG 
CCCCCCCGGG CCCCGAGGCC GCTGGAAGAG AAAGCGGCCC CGGACACCCC GCCTCTGCCC 
CTGCAAATCG ACGGTGCTGA GGAAGCCCCC AAGTCCGGGT CGCCGGACCT GTCCGAGGAC 
GCGTACTTCA TCCAGAAGGG CGCGCAGGAC CTCCTCTCCA CGCCCTACCC CTTCTCGTCG 
CCAATCCACC TGCAGCTGGC CCCCCGCTCC TGCGGCGACG GGTCCCCGTG GCAGCCGCCC 
GCCGACCTGT CGGGACTCTC GGTCGAAGAG GTGTCCAAGT CCCTGCGGTT CATCGGGCTG 
TCGGAGGACG TCATCGTGTT CTTCGTGACC GAGAAGATCG ATGGCAATCT GCTCGTTCAG 
CTCACAGAAG AAATCCTCTC GGAGGATTTC AAACTGAGCA AACTGCAGGT GAAGAAAATT 
CTGCAGTTCA TTAATGGCTG GAGACCCAAA ATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAREM1 ( XM_002697684.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002697730.1 Bos taurus GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa32453
Clone ID Related Accession (Same CDS sequence)	XM_015469095.1 , XM_002697684.4 , XM_002697684.5
Accession Version	XM_002697684.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2616bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	GRB2-associated and regulator of MAPK protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104566.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002697684.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCGG CGCCCTCGCT GGGCTGCAGC CTCAAGGATG TGAAGTGGAG CTCGGTGGCC 
GTTCCGCTCG ACCTGCTGGT CAGCACTTAC CGACTGCCCC AGATTGCGCG GCTGGACAGC 
GGAGAGTGCG TAGAAGGGCT GCGGGACAAT GACTACCTTC TGATCCACTC GTGTCGTCAG 
TGGACCACCA TCACCGCCCA CAGCTTGGAG GAGGGGCACT ATGTCATTGG GCCAAAGATA 
GAGATTCCGG TACATTATGC AGGGCAATTC AAGTTGCTGG AACAAGACCG AGATATAAAG 
GAGCCAGTGC AATATTTCAA CAGCGTGGAG GAGGTGGCCA AAGCATTTCC TGAACGCGTG 
TACGTCATGG AGGAAATAAC ATTCAACGTG AAGGTTGCTT CGGGTGAATG CAATGAAGAC 
ACCGAAGTTT ACAACATCAC CCTGTGTACG GGAGATGAAC TCACTCTAAT GGGCCAGGCA 
GAAATCCTTT ATGCAAAGAC TTTCAAGGAG AAGTCGCGAC TCAACACCAT CTTCAAAAAG 
ATTGGGAAAC TCAACTCCAT CAGCAAGCTG GGAAAAGGCA AGATGCCCTG CCTCATTTGC 
ATGAATCACC GGACCAACGA GAGCATCAGC CTCCCGTTCC AGTGCAAGGG CAGGTTTAGC 
ACCCGGAGCC CCCTGGAGCT TCAGATGCAG GAGGGCGAGC ATACCATCCG CAACATCGTG 
GAGAAAACCA GGCTCCCCGT GAACGTGACG GTGCCCAGCC CACCGCCCAG AAACCCGTAT 
GACCTCCACT TCATCCGCGA GGGGCACCGC TACAAGTTTG TGAACATCCA GACCAAGACA 
GTGGTGGTCT GCTGTGTGCT GCGGAACAAC AAGATCCTCC CTATGCACTT CCCTTTGCAC 
TTGACCGTCC CCAAGTTTAG CCTCCCGGAA CACCTGGTCA AGGGAGAGGG ATGGCCTGAA 
ACCCTGGTCC ATCACTGGCT GGGTATCTGC CAAGAACAGT TCGACATCGA TGAGTATTCA 
CGGGCAGTCC GCGACGTGAA AACCGACTGG AACGAGGACT GTAAGAGCCC CAAGAAAGGC 
CGGTGTGCCG GCCATAACCA CGTGCCCAAT TCCCTTAGCT ACGCTCGCGA TGAGCTCACC 
CAGTCCTTCC ACCGGCTCTC GGTCTGTGTG TATGGCAACA ATCTCCATGG CAACAGCGAG 
GTGAACCTCC ACGGCTGCAG GGACCTGGGA GGAGAGTGGG CCCCCTTTCC TCACGACATC 
CTACCCTATC AGGACTCGGG GGACAGCGGG AGCGACTACC TTTTCCCAGA AGCTGGTGAA 
GAATCTGCAG GCATGGCAGG GAAGTCAGAA CTTCCTTATG AAGAGCTGTG GCTGGAGGAA 
GGCAAGCCCA GCCATCAGCC TCTCACTCGC TCCCTGAGCG AGAAGAGCAG ATGCGACCAG 
TTTCGAGGTT CTGTCCGGTC CAAGTGTGCA ACCTCTCCCC TTCCTGTGCC CGGAGCGCTG 
GGTGCAGCAG TGAAATCTGC AGAGATCGCC CTACCTCCGC CTCCAGTGCC TCCCAAATCC 
GAAGCCGTCC GGGAAGAGTG CCGGCTCCTG AACGCGCCGC CGGTCCCGCC CCGCAGCGCA 
AAGCCTCAGT CCACCAGCCC CTCGGTCCCT CCTCGCACAG CCAAGCCGGC GCGGCCGCAG 
ACTCGCTCGC CCAGCCCCAC CTTGTCCTAC TATTCTTCAG GGCTGCACGA CATCGTCACT 
AAAAGTGAGA CGAGCCCCCC CGAGAGCGCT CCCGTGTCCT GCTACCCGTG TAACCGAGTG 
AAGGCAGATT CCGGGGACCC CAAGTCCCCG TGCGGCAGCC CCCCTTCTGA AGCTCTGTCC 
TCCAGGCTCT CGTGGCCAAA CCATTACTCG GGAGCGTCGG AGACCCACAC CAGGAGCGAC 
TTCCTGCTGG ATCCGAGCCG CAGCTACAGT TACCCCCGAC AGAAGACGCC AGGCACGCCC 
AAGAGAAACT GCCCGGCCGC CTTCGAGCTG GACGGCCGCG AGCTGGCCAC CAGCCCGCCT 
GTTCCGGGCC CCGCGGAGCC CGCGGGCTGT CCCAAGTCGG CCAGCTACTG CCTGGAGCGC 
TCGGGTGACA GGAGCCTGGC AGGCGGGCCG GCCAAGCAGA GCAGCTCCTG CCCCGCCTTG 
CCCCCCCGGG CCCCGAGGCC GCTGGAAGAG AAAGCGGCCC CGGACACCCC GCCTCTGCCC 
CTGCAAATCG ACGGTGCTGA GGAAGCCCCC AAGTCCGGGT CGCCGGACCT GTCCGAGGAC 
GCGTACTTCA TCCAGAAGGG CGCGCAGGAC CTCCTCTCCA CGCCCTACCC CTTCTCGTCG 
CCAATCCACC TGCAGCTGGC CCCCCGCTCC TGCGGCGACG GGTCCCCGTG GCAGCCGCCC 
GCCGACCTGT CGGGACTCTC GGTCGAAGAG GTGTCCAAGT CCCTGCGGTT CATCGGGCTG 
TCGGAGGACG TCATCGTGTT CTTCGTGACC GAGAAGATCG ATGGCAATCT GCTCGTTCAG 
CTCACAGAAG AAATCCTCTC GGAGGATTTC AAACTGAGCA AACTGCAGGT GAAGAAAATT 
CTGCAGTTCA TTAATGGCTG GAGACCCAAA ATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002697730.1
CDS	71..2686
Translation	MDPAPSLGCSLKDVKWSSVAVPLDLLVSTYRLPQIARLDSGECVEGLRDNDYLLIHSCRQWTTITAHSLEEGHYVIGPKIEIPVHYAGQFKLLEQDRDIKEPVQYFNSVEEVAKAFPERVYVMEEITFNVKVASGECNEDTEVYNITLCTGDELTLMGQAEILYAKTFKEKSRLNTIFKKIGKLNSISKLGKGKMPCLICMNHRTNESISLPFQCKGRFSTRSPLELQMQEGEHTIRNIVEKTRLPVNVTVPSPPPRNPYDLHFIREGHRYKFVNIQTKTVVVCCVLRNNKILPMHFPLHLTVPKFSLPEHLVKGEGWPETLVHHWLGICQEQFDIDEYSRAVRDVKTDWNEDCKSPKKGRCAGHNHVPNSLSYARDELTQSFHRLSVCVYGNNLHGNSEVNLHGCRDLGGEWAPFPHDILPYQDSGDSGSDYLFPEAGEESAGMAGKSELPYEELWLEEGKPSHQPLTRSLSEKSRCDQFRGSVRSKCATSPLPVPGALGAAVKSAEIALPPPPVPPKSEAVREECRLLNAPPVPPRSAKPQSTSPSVPPRTAKPARPQTRSPSPTLSYYSSGLHDIVTKSETSPPESAPVSCYPCNRVKADSGDPKSPCGSPPSEALSSRLSWPNHYSGASETHTRSDFLLDPSRSYSYPRQKTPGTPKRNCPAAFELDGRELATSPPVPGPAEPAGCPKSASYCLERSGDRSLAGGPAKQSSSCPALPPRAPRPLEEKAAPDTPPLPLQIDGAEEAPKSGSPDLSEDAYFIQKGAQDLLSTPYPFSSPIHLQLAPRSCGDGSPWQPPADLSGLSVEEVSKSLRFIGLSEDVIVFFVTEKIDGNLLVQLTEEILSEDFKLSKLQVKKILQFINGWRPKI

Target ORF information:

RefSeq Version	XM_002697684.4
Organism	Bos taurus(cattle)
Definition	Bos taurus GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002697684.4

ORF Insert Sequence:

ATGGACCCGG CGCCCTCGCT GGGCTGCAGC CTCAAGGATG TGAAGTGGAG CTCGGTGGCC 
GTTCCGCTCG ACCTGCTGGT CAGCACTTAC CGACTGCCCC AGATTGCGCG GCTGGACAGC 
GGAGAGTGCG TAGAAGGGCT GCGGGACAAT GACTACCTTC TGATCCACTC GTGTCGTCAG 
TGGACCACCA TCACCGCCCA CAGCTTGGAG GAGGGGCACT ATGTCATTGG GCCAAAGATA 
GAGATTCCGG TACATTATGC AGGGCAATTC AAGTTGCTGG AACAAGACCG AGATATAAAG 
GAGCCAGTGC AATATTTCAA CAGCGTGGAG GAGGTGGCCA AAGCATTTCC TGAACGCGTG 
TACGTCATGG AGGAAATAAC ATTCAACGTG AAGGTTGCTT CGGGTGAATG CAATGAAGAC 
ACCGAAGTTT ACAACATCAC CCTGTGTACG GGAGATGAAC TCACTCTAAT GGGCCAGGCA 
GAAATCCTTT ATGCAAAGAC TTTCAAGGAG AAGTCGCGAC TCAACACCAT CTTCAAAAAG 
ATTGGGAAAC TCAACTCCAT CAGCAAGCTG GGAAAAGGCA AGATGCCCTG CCTCATTTGC 
ATGAATCACC GGACCAACGA GAGCATCAGC CTCCCGTTCC AGTGCAAGGG CAGGTTTAGC 
ACCCGGAGCC CCCTGGAGCT TCAGATGCAG GAGGGCGAGC ATACCATCCG CAACATCGTG 
GAGAAAACCA GGCTCCCCGT GAACGTGACG GTGCCCAGCC CACCGCCCAG AAACCCGTAT 
GACCTCCACT TCATCCGCGA GGGGCACCGC TACAAGTTTG TGAACATCCA GACCAAGACA 
GTGGTGGTCT GCTGTGTGCT GCGGAACAAC AAGATCCTCC CTATGCACTT CCCTTTGCAC 
TTGACCGTCC CCAAGTTTAG CCTCCCGGAA CACCTGGTCA AGGGAGAGGG ATGGCCTGAA 
ACCCTGGTCC ATCACTGGCT GGGTATCTGC CAAGAACAGT TCGACATCGA TGAGTATTCA 
CGGGCAGTCC GCGACGTGAA AACCGACTGG AACGAGGACT GTAAGAGCCC CAAGAAAGGC 
CGGTGTGCCG GCCATAACCA CGTGCCCAAT TCCCTTAGCT ACGCTCGCGA TGAGCTCACC 
CAGTCCTTCC ACCGGCTCTC GGTCTGTGTG TATGGCAACA ATCTCCATGG CAACAGCGAG 
GTGAACCTCC ACGGCTGCAG GGACCTGGGA GGAGAGTGGG CCCCCTTTCC TCACGACATC 
CTACCCTATC AGGACTCGGG GGACAGCGGG AGCGACTACC TTTTCCCAGA AGCTGGTGAA 
GAATCTGCAG GCATGGCAGG GAAGTCAGAA CTTCCTTATG AAGAGCTGTG GCTGGAGGAA 
GGCAAGCCCA GCCATCAGCC TCTCACTCGC TCCCTGAGCG AGAAGAGCAG ATGCGACCAG 
TTTCGAGGTT CTGTCCGGTC CAAGTGTGCA ACCTCTCCCC TTCCTGTGCC CGGAGCGCTG 
GGTGCAGCAG TGAAATCTGC AGAGATCGCC CTACCTCCGC CTCCAGTGCC TCCCAAATCC 
GAAGCCGTCC GGGAAGAGTG CCGGCTCCTG AACGCGCCGC CGGTCCCGCC CCGCAGCGCA 
AAGCCTCAGT CCACCAGCCC CTCGGTCCCT CCTCGCACAG CCAAGCCGGC GCGGCCGCAG 
ACTCGCTCGC CCAGCCCCAC CTTGTCCTAC TATTCTTCAG GGCTGCACGA CATCGTCACT 
AAAAGTGAGA CGAGCCCCCC CGAGAGCGCT CCCGTGTCCT GCTACCCGTG TAACCGAGTG 
AAGGCAGATT CCGGGGACCC CAAGTCCCCG TGCGGCAGCC CCCCTTCTGA AGCTCTGTCC 
TCCAGGCTCT CGTGGCCAAA CCATTACTCG GGAGCGTCGG AGACCCACAC CAGGAGCGAC 
TTCCTGCTGG ATCCGAGCCG CAGCTACAGT TACCCCCGAC AGAAGACGCC AGGCACGCCC 
AAGAGAAACT GCCCGGCCGC CTTCGAGCTG GACGGCCGCG AGCTGGCCAC CAGCCCGCCT 
GTTCCGGGCC CCGCGGAGCC CGCGGGCTGT CCCAAGTCGG CCAGCTACTG CCTGGAGCGC 
TCGGGTGACA GGAGCCTGGC AGGCGGGCCG GCCAAGCAGA GCAGCTCCTG CCCCGCCTTG 
CCCCCCCGGG CCCCGAGGCC GCTGGAAGAG AAAGCGGCCC CGGACACCCC GCCTCTGCCC 
CTGCAAATCG ACGGTGCTGA GGAAGCCCCC AAGTCCGGGT CGCCGGACCT GTCCGAGGAC 
GCGTACTTCA TCCAGAAGGG CGCGCAGGAC CTCCTCTCCA CGCCCTACCC CTTCTCGTCG 
CCAATCCACC TGCAGCTGGC CCCCCGCTCC TGCGGCGACG GGTCCCCGTG GCAGCCGCCC 
GCCGACCTGT CGGGACTCTC GGTCGAAGAG GTGTCCAAGT CCCTGCGGTT CATCGGGCTG 
TCGGAGGACG TCATCGTGTT CTTCGTGACC GAGAAGATCG ATGGCAATCT GCTCGTTCAG 
CTCACAGAAG AAATCCTCTC GGAGGATTTC AAACTGAGCA AACTGCAGGT GAAGAAAATT 
CTGCAGTTCA TTAATGGCTG GAGACCCAAA ATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAREM1 ( XM_002697684.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002697730.1 Bos taurus GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa32453
Clone ID Related Accession (Same CDS sequence)	XM_015469095.1 , XM_002697684.4 , XM_002697684.5
Accession Version	XM_002697684.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2616bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	GRB2-associated and regulator of MAPK protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002697684.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCGG CGCCCTCGCT GGGCTGCAGC CTCAAGGATG TGAAGTGGAG CTCGGTGGCC 
GTTCCGCTCG ACCTGCTGGT CAGCACTTAC CGACTGCCCC AGATTGCGCG GCTGGACAGC 
GGAGAGTGCG TAGAAGGGCT GCGGGACAAT GACTACCTTC TGATCCACTC GTGTCGTCAG 
TGGACCACCA TCACCGCCCA CAGCTTGGAG GAGGGGCACT ATGTCATTGG GCCAAAGATA 
GAGATTCCGG TACATTATGC AGGGCAATTC AAGTTGCTGG AACAAGACCG AGATATAAAG 
GAGCCAGTGC AATATTTCAA CAGCGTGGAG GAGGTGGCCA AAGCATTTCC TGAACGCGTG 
TACGTCATGG AGGAAATAAC ATTCAACGTG AAGGTTGCTT CGGGTGAATG CAATGAAGAC 
ACCGAAGTTT ACAACATCAC CCTGTGTACG GGAGATGAAC TCACTCTAAT GGGCCAGGCA 
GAAATCCTTT ATGCAAAGAC TTTCAAGGAG AAGTCGCGAC TCAACACCAT CTTCAAAAAG 
ATTGGGAAAC TCAACTCCAT CAGCAAGCTG GGAAAAGGCA AGATGCCCTG CCTCATTTGC 
ATGAATCACC GGACCAACGA GAGCATCAGC CTCCCGTTCC AGTGCAAGGG CAGGTTTAGC 
ACCCGGAGCC CCCTGGAGCT TCAGATGCAG GAGGGCGAGC ATACCATCCG CAACATCGTG 
GAGAAAACCA GGCTCCCCGT GAACGTGACG GTGCCCAGCC CACCGCCCAG AAACCCGTAT 
GACCTCCACT TCATCCGCGA GGGGCACCGC TACAAGTTTG TGAACATCCA GACCAAGACA 
GTGGTGGTCT GCTGTGTGCT GCGGAACAAC AAGATCCTCC CTATGCACTT CCCTTTGCAC 
TTGACCGTCC CCAAGTTTAG CCTCCCGGAA CACCTGGTCA AGGGAGAGGG ATGGCCTGAA 
ACCCTGGTCC ATCACTGGCT GGGTATCTGC CAAGAACAGT TCGACATCGA TGAGTATTCA 
CGGGCAGTCC GCGACGTGAA AACCGACTGG AACGAGGACT GTAAGAGCCC CAAGAAAGGC 
CGGTGTGCCG GCCATAACCA CGTGCCCAAT TCCCTTAGCT ACGCTCGCGA TGAGCTCACC 
CAGTCCTTCC ACCGGCTCTC GGTCTGTGTG TATGGCAACA ATCTCCATGG CAACAGCGAG 
GTGAACCTCC ACGGCTGCAG GGACCTGGGA GGAGAGTGGG CCCCCTTTCC TCACGACATC 
CTACCCTATC AGGACTCGGG GGACAGCGGG AGCGACTACC TTTTCCCAGA AGCTGGTGAA 
GAATCTGCAG GCATGGCAGG GAAGTCAGAA CTTCCTTATG AAGAGCTGTG GCTGGAGGAA 
GGCAAGCCCA GCCATCAGCC TCTCACTCGC TCCCTGAGCG AGAAGAGCAG ATGCGACCAG 
TTTCGAGGTT CTGTCCGGTC CAAGTGTGCA ACCTCTCCCC TTCCTGTGCC CGGAGCGCTG 
GGTGCAGCAG TGAAATCTGC AGAGATCGCC CTACCTCCGC CTCCAGTGCC TCCCAAATCC 
GAAGCCGTCC GGGAAGAGTG CCGGCTCCTG AACGCGCCGC CGGTCCCGCC CCGCAGCGCA 
AAGCCTCAGT CCACCAGCCC CTCGGTCCCT CCTCGCACAG CCAAGCCGGC GCGGCCGCAG 
ACTCGCTCGC CCAGCCCCAC CTTGTCCTAC TATTCTTCAG GGCTGCACGA CATCGTCACT 
AAAAGTGAGA CGAGCCCCCC CGAGAGCGCT CCCGTGTCCT GCTACCCGTG TAACCGAGTG 
AAGGCAGATT CCGGGGACCC CAAGTCCCCG TGCGGCAGCC CCCCTTCTGA AGCTCTGTCC 
TCCAGGCTCT CGTGGCCAAA CCATTACTCG GGAGCGTCGG AGACCCACAC CAGGAGCGAC 
TTCCTGCTGG ATCCGAGCCG CAGCTACAGT TACCCCCGAC AGAAGACGCC AGGCACGCCC 
AAGAGAAACT GCCCGGCCGC CTTCGAGCTG GACGGCCGCG AGCTGGCCAC CAGCCCGCCT 
GTTCCGGGCC CCGCGGAGCC CGCGGGCTGT CCCAAGTCGG CCAGCTACTG CCTGGAGCGC 
TCGGGTGACA GGAGCCTGGC AGGCGGGCCG GCCAAGCAGA GCAGCTCCTG CCCCGCCTTG 
CCCCCCCGGG CCCCGAGGCC GCTGGAAGAG AAAGCGGCCC CGGACACCCC GCCTCTGCCC 
CTGCAAATCG ACGGTGCTGA GGAAGCCCCC AAGTCCGGGT CGCCGGACCT GTCCGAGGAC 
GCGTACTTCA TCCAGAAGGG CGCGCAGGAC CTCCTCTCCA CGCCCTACCC CTTCTCGTCG 
CCAATCCACC TGCAGCTGGC CCCCCGCTCC TGCGGCGACG GGTCCCCGTG GCAGCCGCCC 
GCCGACCTGT CGGGACTCTC GGTCGAAGAG GTGTCCAAGT CCCTGCGGTT CATCGGGCTG 
TCGGAGGACG TCATCGTGTT CTTCGTGACC GAGAAGATCG ATGGCAATCT GCTCGTTCAG 
CTCACAGAAG AAATCCTCTC GGAGGATTTC AAACTGAGCA AACTGCAGGT GAAGAAAATT 
CTGCAGTTCA TTAATGGCTG GAGACCCAAA ATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002697730.1
CDS	455..3070
Translation	MDPAPSLGCSLKDVKWSSVAVPLDLLVSTYRLPQIARLDSGECVEGLRDNDYLLIHSCRQWTTITAHSLEEGHYVIGPKIEIPVHYAGQFKLLEQDRDIKEPVQYFNSVEEVAKAFPERVYVMEEITFNVKVASGECNEDTEVYNITLCTGDELTLMGQAEILYAKTFKEKSRLNTIFKKIGKLNSISKLGKGKMPCLICMNHRTNESISLPFQCKGRFSTRSPLELQMQEGEHTIRNIVEKTRLPVNVTVPSPPPRNPYDLHFIREGHRYKFVNIQTKTVVVCCVLRNNKILPMHFPLHLTVPKFSLPEHLVKGEGWPETLVHHWLGICQEQFDIDEYSRAVRDVKTDWNEDCKSPKKGRCAGHNHVPNSLSYARDELTQSFHRLSVCVYGNNLHGNSEVNLHGCRDLGGEWAPFPHDILPYQDSGDSGSDYLFPEAGEESAGMAGKSELPYEELWLEEGKPSHQPLTRSLSEKSRCDQFRGSVRSKCATSPLPVPGALGAAVKSAEIALPPPPVPPKSEAVREECRLLNAPPVPPRSAKPQSTSPSVPPRTAKPARPQTRSPSPTLSYYSSGLHDIVTKSETSPPESAPVSCYPCNRVKADSGDPKSPCGSPPSEALSSRLSWPNHYSGASETHTRSDFLLDPSRSYSYPRQKTPGTPKRNCPAAFELDGRELATSPPVPGPAEPAGCPKSASYCLERSGDRSLAGGPAKQSSSCPALPPRAPRPLEEKAAPDTPPLPLQIDGAEEAPKSGSPDLSEDAYFIQKGAQDLLSTPYPFSSPIHLQLAPRSCGDGSPWQPPADLSGLSVEEVSKSLRFIGLSEDVIVFFVTEKIDGNLLVQLTEEILSEDFKLSKLQVKKILQFINGWRPKI

Target ORF information:

RefSeq Version	XM_002697684.5
Organism	Bos taurus(cattle)
Definition	Bos taurus GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002697684.5

ORF Insert Sequence:

ATGGACCCGG CGCCCTCGCT GGGCTGCAGC CTCAAGGATG TGAAGTGGAG CTCGGTGGCC 
GTTCCGCTCG ACCTGCTGGT CAGCACTTAC CGACTGCCCC AGATTGCGCG GCTGGACAGC 
GGAGAGTGCG TAGAAGGGCT GCGGGACAAT GACTACCTTC TGATCCACTC GTGTCGTCAG 
TGGACCACCA TCACCGCCCA CAGCTTGGAG GAGGGGCACT ATGTCATTGG GCCAAAGATA 
GAGATTCCGG TACATTATGC AGGGCAATTC AAGTTGCTGG AACAAGACCG AGATATAAAG 
GAGCCAGTGC AATATTTCAA CAGCGTGGAG GAGGTGGCCA AAGCATTTCC TGAACGCGTG 
TACGTCATGG AGGAAATAAC ATTCAACGTG AAGGTTGCTT CGGGTGAATG CAATGAAGAC 
ACCGAAGTTT ACAACATCAC CCTGTGTACG GGAGATGAAC TCACTCTAAT GGGCCAGGCA 
GAAATCCTTT ATGCAAAGAC TTTCAAGGAG AAGTCGCGAC TCAACACCAT CTTCAAAAAG 
ATTGGGAAAC TCAACTCCAT CAGCAAGCTG GGAAAAGGCA AGATGCCCTG CCTCATTTGC 
ATGAATCACC GGACCAACGA GAGCATCAGC CTCCCGTTCC AGTGCAAGGG CAGGTTTAGC 
ACCCGGAGCC CCCTGGAGCT TCAGATGCAG GAGGGCGAGC ATACCATCCG CAACATCGTG 
GAGAAAACCA GGCTCCCCGT GAACGTGACG GTGCCCAGCC CACCGCCCAG AAACCCGTAT 
GACCTCCACT TCATCCGCGA GGGGCACCGC TACAAGTTTG TGAACATCCA GACCAAGACA 
GTGGTGGTCT GCTGTGTGCT GCGGAACAAC AAGATCCTCC CTATGCACTT CCCTTTGCAC 
TTGACCGTCC CCAAGTTTAG CCTCCCGGAA CACCTGGTCA AGGGAGAGGG ATGGCCTGAA 
ACCCTGGTCC ATCACTGGCT GGGTATCTGC CAAGAACAGT TCGACATCGA TGAGTATTCA 
CGGGCAGTCC GCGACGTGAA AACCGACTGG AACGAGGACT GTAAGAGCCC CAAGAAAGGC 
CGGTGTGCCG GCCATAACCA CGTGCCCAAT TCCCTTAGCT ACGCTCGCGA TGAGCTCACC 
CAGTCCTTCC ACCGGCTCTC GGTCTGTGTG TATGGCAACA ATCTCCATGG CAACAGCGAG 
GTGAACCTCC ACGGCTGCAG GGACCTGGGA GGAGAGTGGG CCCCCTTTCC TCACGACATC 
CTACCCTATC AGGACTCGGG GGACAGCGGG AGCGACTACC TTTTCCCAGA AGCTGGTGAA 
GAATCTGCAG GCATGGCAGG GAAGTCAGAA CTTCCTTATG AAGAGCTGTG GCTGGAGGAA 
GGCAAGCCCA GCCATCAGCC TCTCACTCGC TCCCTGAGCG AGAAGAGCAG ATGCGACCAG 
TTTCGAGGTT CTGTCCGGTC CAAGTGTGCA ACCTCTCCCC TTCCTGTGCC CGGAGCGCTG 
GGTGCAGCAG TGAAATCTGC AGAGATCGCC CTACCTCCGC CTCCAGTGCC TCCCAAATCC 
GAAGCCGTCC GGGAAGAGTG CCGGCTCCTG AACGCGCCGC CGGTCCCGCC CCGCAGCGCA 
AAGCCTCAGT CCACCAGCCC CTCGGTCCCT CCTCGCACAG CCAAGCCGGC GCGGCCGCAG 
ACTCGCTCGC CCAGCCCCAC CTTGTCCTAC TATTCTTCAG GGCTGCACGA CATCGTCACT 
AAAAGTGAGA CGAGCCCCCC CGAGAGCGCT CCCGTGTCCT GCTACCCGTG TAACCGAGTG 
AAGGCAGATT CCGGGGACCC CAAGTCCCCG TGCGGCAGCC CCCCTTCTGA AGCTCTGTCC 
TCCAGGCTCT CGTGGCCAAA CCATTACTCG GGAGCGTCGG AGACCCACAC CAGGAGCGAC 
TTCCTGCTGG ATCCGAGCCG CAGCTACAGT TACCCCCGAC AGAAGACGCC AGGCACGCCC 
AAGAGAAACT GCCCGGCCGC CTTCGAGCTG GACGGCCGCG AGCTGGCCAC CAGCCCGCCT 
GTTCCGGGCC CCGCGGAGCC CGCGGGCTGT CCCAAGTCGG CCAGCTACTG CCTGGAGCGC 
TCGGGTGACA GGAGCCTGGC AGGCGGGCCG GCCAAGCAGA GCAGCTCCTG CCCCGCCTTG 
CCCCCCCGGG CCCCGAGGCC GCTGGAAGAG AAAGCGGCCC CGGACACCCC GCCTCTGCCC 
CTGCAAATCG ACGGTGCTGA GGAAGCCCCC AAGTCCGGGT CGCCGGACCT GTCCGAGGAC 
GCGTACTTCA TCCAGAAGGG CGCGCAGGAC CTCCTCTCCA CGCCCTACCC CTTCTCGTCG 
CCAATCCACC TGCAGCTGGC CCCCCGCTCC TGCGGCGACG GGTCCCCGTG GCAGCCGCCC 
GCCGACCTGT CGGGACTCTC GGTCGAAGAG GTGTCCAAGT CCCTGCGGTT CATCGGGCTG 
TCGGAGGACG TCATCGTGTT CTTCGTGACC GAGAAGATCG ATGGCAATCT GCTCGTTCAG 
CTCACAGAAG AAATCCTCTC GGAGGATTTC AAACTGAGCA AACTGCAGGT GAAGAAAATT 
CTGCAGTTCA TTAATGGCTG GAGACCCAAA ATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAREM1 ( XM_015469096.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015324582.1 Bos taurus GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant X3, mRNA.

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CloneID	OBa95180
Clone ID Related Accession (Same CDS sequence)	XM_015469096.1
Accession Version	XM_015469096.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2253bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	GRB2-associated and regulator of MAPK protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646632.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAAA TAACATTCAA CGTGAAGGTT GCTTCGGGTG AATGCAATGA AGACACCGAA 
GTTTACAACA TCACCCTGTG TACGGGAGAT GAACTCACTC TAATGGGCCA GGCAGAAATC 
CTTTATGCAA AGACTTTCAA GGAGAAGTCG CGACTCAACA CCATCTTCAA AAAGATTGGG 
AAACTCAACT CCATCAGCAA GCTGGGAAAA GGCAAGATGC CCTGCCTCAT TTGCATGAAT 
CACCGGACCA ACGAGAGCAT CAGCCTCCCG TTCCAGTGCA AGGGCAGGTT TAGCACCCGG 
AGCCCCCTGG AGCTTCAGAT GCAGGAGGGC GAGCATACCA TCCGCAACAT CGTGGAGAAA 
ACCAGGCTCC CCGTGAACGT GACGGTGCCC AGCCCACCGC CCAGAAACCC GTATGACCTC 
CACTTCATCC GCGAGGGGCA CCGCTACAAG TTTGTGAACA TCCAGACCAA GACAGTGGTG 
GTCTGCTGTG TGCTGCGGAA CAACAAGATC CTCCCTATGC ACTTCCCTTT GCACTTGACC 
GTCCCCAAGT TTAGCCTCCC GGAACACCTG GTCAAGGGAG AGGGATGGCC TGAAACCCTG 
GTCCATCACT GGCTGGGTAT CTGCCAAGAA CAGTTCGACA TCGATGAGTA TTCACGGGCA 
GTCCGCGACG TGAAAACCGA CTGGAACGAG GACTGTAAGA GCCCCAAGAA AGGCCGGTGT 
GCCGGCCATA ACCACGTGCC CAATTCCCTT AGCTACGCTC GCGATGAGCT CACCCAGTCC 
TTCCACCGGC TCTCGGTCTG TGTGTATGGC AACAATCTCC ATGGCAACAG CGAGGTGAAC 
CTCCACGGCT GCAGGGACCT GGGAGGAGAG TGGGCCCCCT TTCCTCACGA CATCCTACCC 
TATCAGGACT CGGGGGACAG CGGGAGCGAC TACCTTTTCC CAGAAGCTGG TGAAGAATCT 
GCAGGCATGG CAGGGAAGTC AGAACTTCCT TATGAAGAGC TGTGGCTGGA GGAAGGCAAG 
CCCAGCCATC AGCCTCTCAC TCGCTCCCTG AGCGAGAAGA GCAGATGCGA CCAGTTTCGA 
GGTTCTGTCC GGTCCAAGTG TGCAACCTCT CCCCTTCCTG TGCCCGGAGC GCTGGGTGCA 
GCAGTGAAAT CTGCAGAGAT CGCCCTACCT CCGCCTCCAG TGCCTCCCAA ATCCGAAGCC 
GTCCGGGAAG AGTGCCGGCT CCTGAACGCG CCGCCGGTCC CGCCCCGCAG CGCAAAGCCT 
CAGTCCACCA GCCCCTCGGT CCCTCCTCGC ACAGCCAAGC CGGCGCGGCC GCAGACTCGC 
TCGCCCAGCC CCACCTTGTC CTACTATTCT TCAGGGCTGC ACGACATCAG CGTCACTAAA 
AGTGAGACGA GCCCCCCCGA GAGCGCTCCC GTGTCCTGCT ACCCGTGTAA CCGAGTGAAG 
GCAGATTCCG GGGACCCCAA GTCCCCGTGC GGCAGCCCCC CTTCTGAAGC TCTGTCCTCC 
AGGCTCTCGT GGCCAAACCA TTACTCGGGA GCGTCGGAGA CCCACACCAG GAGCGACTTC 
CTGCTGGATC CGAGCCGCAG CTACAGTTAC CCCCGACAGA AGACGCCAGG CACGCCCAAG 
AGAAACTGCC CGGCCGCCTT CGAGCTGGAC GGCCGCGAGC TGGCCACCAG CCCGCCTGTT 
CCGGGCCCCG CGGAGCCCGC GGGCTGTCCC AAGTCGGCCA GCTACTGCCT GGAGCGCTCG 
GGTGACAGGA GCCTGGCAGG CGGGCCGGCC AAGCAGAGCA GCTCCTGCCC CGCCTTGCCC 
CCCCGGGCCC CGAGGCCGCT GGAAGAGAAA GCGGCCCCGG ACACCCCGCC TCTGCCCCTG 
CAAATCGACG GTGCTGAGGA AGCCCCCAAG TCCGGGTCGC CGGACCTGTC CGAGGACGCG 
TACTTCATCC AGAAGGGCGC GCAGGACCTC CTCTCCACGC CCTACCCCTT CTCGTCGCCA 
ATCCACCTGC AGCTGGCCCC CCGCTCCTGC GGCGACGGGT CCCCGTGGCA GCCGCCCGCC 
GACCTGTCGG GACTCTCGGT CGAAGAGGTG TCCAAGTCCC TGCGGTTCAT CGGGCTGTCG 
GAGGACGTCA TCGTGTTCTT CGTGACCGAG AAGATCGATG GCAATCTGCT CGTTCAGCTC 
ACAGAAGAAA TCCTCTCGGA GGATTTCAAA CTGAGCAAAC TGCAGGTGAA GAAAATTCTG 
CAGTTCATTA ATGGCTGGAG ACCCAAAATA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015324582.1
CDS	189..2441
Translation	MEEITFNVKVASGECNEDTEVYNITLCTGDELTLMGQAEILYAKTFKEKSRLNTIFKKIGKLNSISKLGKGKMPCLICMNHRTNESISLPFQCKGRFSTRSPLELQMQEGEHTIRNIVEKTRLPVNVTVPSPPPRNPYDLHFIREGHRYKFVNIQTKTVVVCCVLRNNKILPMHFPLHLTVPKFSLPEHLVKGEGWPETLVHHWLGICQEQFDIDEYSRAVRDVKTDWNEDCKSPKKGRCAGHNHVPNSLSYARDELTQSFHRLSVCVYGNNLHGNSEVNLHGCRDLGGEWAPFPHDILPYQDSGDSGSDYLFPEAGEESAGMAGKSELPYEELWLEEGKPSHQPLTRSLSEKSRCDQFRGSVRSKCATSPLPVPGALGAAVKSAEIALPPPPVPPKSEAVREECRLLNAPPVPPRSAKPQSTSPSVPPRTAKPARPQTRSPSPTLSYYSSGLHDISVTKSETSPPESAPVSCYPCNRVKADSGDPKSPCGSPPSEALSSRLSWPNHYSGASETHTRSDFLLDPSRSYSYPRQKTPGTPKRNCPAAFELDGRELATSPPVPGPAEPAGCPKSASYCLERSGDRSLAGGPAKQSSSCPALPPRAPRPLEEKAAPDTPPLPLQIDGAEEAPKSGSPDLSEDAYFIQKGAQDLLSTPYPFSSPIHLQLAPRSCGDGSPWQPPADLSGLSVEEVSKSLRFIGLSEDVIVFFVTEKIDGNLLVQLTEEILSEDFKLSKLQVKKILQFINGWRPKI

Target ORF information:

RefSeq Version	XM_015469096.1
Organism	Bos taurus(cattle)
Definition	Bos taurus GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015469096.1

ORF Insert Sequence:

ATGGAGGAAA TAACATTCAA CGTGAAGGTT GCTTCGGGTG AATGCAATGA AGACACCGAA 
GTTTACAACA TCACCCTGTG TACGGGAGAT GAACTCACTC TAATGGGCCA GGCAGAAATC 
CTTTATGCAA AGACTTTCAA GGAGAAGTCG CGACTCAACA CCATCTTCAA AAAGATTGGG 
AAACTCAACT CCATCAGCAA GCTGGGAAAA GGCAAGATGC CCTGCCTCAT TTGCATGAAT 
CACCGGACCA ACGAGAGCAT CAGCCTCCCG TTCCAGTGCA AGGGCAGGTT TAGCACCCGG 
AGCCCCCTGG AGCTTCAGAT GCAGGAGGGC GAGCATACCA TCCGCAACAT CGTGGAGAAA 
ACCAGGCTCC CCGTGAACGT GACGGTGCCC AGCCCACCGC CCAGAAACCC GTATGACCTC 
CACTTCATCC GCGAGGGGCA CCGCTACAAG TTTGTGAACA TCCAGACCAA GACAGTGGTG 
GTCTGCTGTG TGCTGCGGAA CAACAAGATC CTCCCTATGC ACTTCCCTTT GCACTTGACC 
GTCCCCAAGT TTAGCCTCCC GGAACACCTG GTCAAGGGAG AGGGATGGCC TGAAACCCTG 
GTCCATCACT GGCTGGGTAT CTGCCAAGAA CAGTTCGACA TCGATGAGTA TTCACGGGCA 
GTCCGCGACG TGAAAACCGA CTGGAACGAG GACTGTAAGA GCCCCAAGAA AGGCCGGTGT 
GCCGGCCATA ACCACGTGCC CAATTCCCTT AGCTACGCTC GCGATGAGCT CACCCAGTCC 
TTCCACCGGC TCTCGGTCTG TGTGTATGGC AACAATCTCC ATGGCAACAG CGAGGTGAAC 
CTCCACGGCT GCAGGGACCT GGGAGGAGAG TGGGCCCCCT TTCCTCACGA CATCCTACCC 
TATCAGGACT CGGGGGACAG CGGGAGCGAC TACCTTTTCC CAGAAGCTGG TGAAGAATCT 
GCAGGCATGG CAGGGAAGTC AGAACTTCCT TATGAAGAGC TGTGGCTGGA GGAAGGCAAG 
CCCAGCCATC AGCCTCTCAC TCGCTCCCTG AGCGAGAAGA GCAGATGCGA CCAGTTTCGA 
GGTTCTGTCC GGTCCAAGTG TGCAACCTCT CCCCTTCCTG TGCCCGGAGC GCTGGGTGCA 
GCAGTGAAAT CTGCAGAGAT CGCCCTACCT CCGCCTCCAG TGCCTCCCAA ATCCGAAGCC 
GTCCGGGAAG AGTGCCGGCT CCTGAACGCG CCGCCGGTCC CGCCCCGCAG CGCAAAGCCT 
CAGTCCACCA GCCCCTCGGT CCCTCCTCGC ACAGCCAAGC CGGCGCGGCC GCAGACTCGC 
TCGCCCAGCC CCACCTTGTC CTACTATTCT TCAGGGCTGC ACGACATCAG CGTCACTAAA 
AGTGAGACGA GCCCCCCCGA GAGCGCTCCC GTGTCCTGCT ACCCGTGTAA CCGAGTGAAG 
GCAGATTCCG GGGACCCCAA GTCCCCGTGC GGCAGCCCCC CTTCTGAAGC TCTGTCCTCC 
AGGCTCTCGT GGCCAAACCA TTACTCGGGA GCGTCGGAGA CCCACACCAG GAGCGACTTC 
CTGCTGGATC CGAGCCGCAG CTACAGTTAC CCCCGACAGA AGACGCCAGG CACGCCCAAG 
AGAAACTGCC CGGCCGCCTT CGAGCTGGAC GGCCGCGAGC TGGCCACCAG CCCGCCTGTT 
CCGGGCCCCG CGGAGCCCGC GGGCTGTCCC AAGTCGGCCA GCTACTGCCT GGAGCGCTCG 
GGTGACAGGA GCCTGGCAGG CGGGCCGGCC AAGCAGAGCA GCTCCTGCCC CGCCTTGCCC 
CCCCGGGCCC CGAGGCCGCT GGAAGAGAAA GCGGCCCCGG ACACCCCGCC TCTGCCCCTG 
CAAATCGACG GTGCTGAGGA AGCCCCCAAG TCCGGGTCGC CGGACCTGTC CGAGGACGCG 
TACTTCATCC AGAAGGGCGC GCAGGACCTC CTCTCCACGC CCTACCCCTT CTCGTCGCCA 
ATCCACCTGC AGCTGGCCCC CCGCTCCTGC GGCGACGGGT CCCCGTGGCA GCCGCCCGCC 
GACCTGTCGG GACTCTCGGT CGAAGAGGTG TCCAAGTCCC TGCGGTTCAT CGGGCTGTCG 
GAGGACGTCA TCGTGTTCTT CGTGACCGAG AAGATCGATG GCAATCTGCT CGTTCAGCTC 
ACAGAAGAAA TCCTCTCGGA GGATTTCAAA CTGAGCAAAC TGCAGGTGAA GAAAATTCTG 
CAGTTCATTA ATGGCTGGAG ACCCAAAATA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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GAREM1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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