CPT1A cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	CPT1A
Entrez Gene ID	506812
Full Name	carnitine palmitoyltransferase 1A
General protein information	Preferred Names carnitine palmitoyltransferase 1A Names carnitine O-palmitoyltransferase 1, liver isoform mitochondrial carnitine palmitoyltransferase 1A
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001304989.1	NP_001291918.1	carnitine O-palmitoyltransferase 1, liver isoform
NM_001304989.2	NP_001291918.1	carnitine O-palmitoyltransferase 1, liver isoform
XM_024987034.1	XP_024842802.1	carnitine O-palmitoyltransferase 1, liver isoform isoform X2
XM_005227376.3	XP_005227433.1	carnitine O-palmitoyltransferase 1, liver isoform isoform X1
XM_005227376.4	XP_005227433.1	carnitine O-palmitoyltransferase 1, liver isoform isoform X1
XM_010821269.2	XP_010819571.1	carnitine O-palmitoyltransferase 1, liver isoform isoform X2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Cpt1a	NP_038523.2
Bos taurus (cattle)	CPT1A	XP_005227433.1
Gallus gallus (chicken)	CPT1A	NP_001012916.1
Danio rerio (zebrafish)	si:ch211-236l14.3	XP_005170764.1
	CPT1A	XP_001101846.1
Rattus norvegicus (Norway rat)	Cpt1a	NP_113747.2
Danio rerio (zebrafish)	cpt1a	NP_001038319.1
Pan troglodytes (chimpanzee)	CPT1A	XP_001173853.1
Canis lupus familiaris (dog)	CPT1A	XP_533208.3
Homo sapiens (human)	CPT1A	NP_001867.2
Xenopus tropicalis (tropical clawed frog)	LOC100490391	XP_004913491.1

Gene Ontology
Bibliography

Related articles in PubMed

Effect of NEFA and glucose levels on CPT-I mRNA expression and translation in cultured bovine hepatocytes.
Xu C, Wang Z, Zhang RH, Zhang HY, Fu SX, Xia C
The Journal of veterinary medical science73(1)97-101(2011 Jan)

Activity of hepatic but not skeletal muscle carnitine palmitoyltransferase enzyme is depressed by intravenous glucose infusions in lactating dairy cows.
Al-Trad B, Wittek T, G?bel G, F?rll M, Reisberg K, Aschenbach JR
Journal of animal physiology and animal nutrition94(6)685-95(2010 Dec)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study evaluated the effects of nonesterified fatty acids and glucose on carnitine palmitoyltransferase-I (CPT-I) mRNA expression in cultured bovine hepatocytes using real-time reverse transcription polymerase chain reaction and ELISA methods.
Title: Effect of NEFA and glucose levels on CPT-I mRNA expression and translation in cultured bovine hepatocytes.

It was conclude that suppression of CPT activity by positive energy balance appears to be specific for the liver in mid-lactating dairy cows.
Title: Activity of hepatic but not skeletal muscle carnitine palmitoyltransferase enzyme is depressed by intravenous glucose infusions in lactating dairy cows.

The following CPT1A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CPT1A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa46677	XM_010821269.2 Latest version!	Bos taurus carnitine palmitoyltransferase 1A (liver) (CPT1A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa35037	NM_001304989.1 Latest version!	Bos taurus carnitine palmitoyltransferase 1A (CPT1A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa35037	XM_005227376.3 Latest version!	Bos taurus carnitine palmitoyltransferase 1A (liver) (CPT1A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa231884	NM_001304989.2 Latest version!	Bos taurus carnitine palmitoyltransferase 1A (CPT1A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa231884	XM_005227376.4 Latest version!	Bos taurus carnitine palmitoyltransferase 1A (CPT1A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa231885	XM_024987034.1 Latest version!	Bos taurus carnitine palmitoyltransferase 1A (CPT1A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CPT1A ( XM_010821269.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010819571.1 Bos taurus carnitine palmitoyltransferase 1A (liver) (CPT1A), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa46677
Clone ID Related Accession (Same CDS sequence)	XM_010821269.2
Accession Version	XM_010821269.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1866bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	carnitine O-palmitoyltransferase 1, liver isoform isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104648.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010821269.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAAGA TCTTCTCAGG CCGAAAACCC ATGTTGTACA GCTTCCAGAC GTCTCTGCCG 
CGCCTGCCCG TGCCAGCTGT CCAGGACACC GTCAACAGGT ACTTGGAATC TGTGAAGCCT 
CTTATGAAGG AGGGAGACTT TACACGGATG ACGGCTCTGG CACAAGATTT TGCTGTCAAT 
CTGGGACCAA GATTGCAGTG GTATTTGAAG CTAAAATCCT GGTGGGCTAC CAATTATGTG 
AGCGACTGGT GGGAGGAATA CATCTACCTC CGAGGACGAG GGCCGCTGAT GGTGAACAGC 
AACTACTTCG CGATGGACTT GCTGTATATC ATACCGACTC ACATCCAGGC GGCAAGAGCT 
GGCAACGGGA TCCACGCCAT CCTGCTTTAC AGACGCAAAC TGGACCGGGA GGAAATCAAA 
CCGATTCTTC TTCTGGGGTC TACGATTCCG CTCTGCTCCG CCCAGTGGGA GCGCATGTTT 
AATACTTCTC GGATCCCGGG AGAGGAGACA GACACCATCC AGCACCTGAG GGACAGCAAG 
CACATCGTCG TCTTCCACCG GGGCCGCTAC TTCAAGGTCT GGCTCTACCA CGACGGCCGG 
CTGCTGAGGC CCCGCGAGAT CGAGCAGCAG GTGCAGCGGA TCCTGGAGGA CCCGTCGGAG 
CCGCAGCCCG GGGAGGCCAA GCTGGCGGCG CTTACGGCGG CGGACAGGGT CCCCTGGGCC 
AGGTGTCGCC AGGCCTATTT TGGACACGGG AAAAACAAGC AGTCTCTGGA TGCCGTGGAA 
AAAGCAGCGT TCTTCGTGAC GTTAGACGAA ACCGAGCAGG GATACAGGGA GGAGGACCCG 
GAAACGTCGA TGGACAGCTA CGCCAAGTCC CTGCTGCATG GCAGGTGTTT CGACAGGTGG 
TTTGATAAAT CATTCACGTT TATCGTCTTT AAAAACGGAA AGATGGGCAT TAATGCGGAG 
CACTCCTGGG CCGATGCGCC CATCATGGGT CACCTTTGGG AGTACGTCAT GGCCACAGAC 
AGCTTCCAGC TGGGTTACAC GGAGGATGGA CACTGTAAAG GAGACACCAA CCCGAACATC 
CCGTACCCTA CCCGGCTGCA GTGGGACATC CCGGAGGAAT GTCAGGAGGT CATTGAGACT 
TCACTGAGCA GTGCCAACCT CCTGGCCAAC GACGTGGATT TCCACTCCTT CCCATTCCGC 
ACTTTCGGTA AAGGGCTGAT TAAGAAATGT AAAACCAGCC CGGACGCCTT CGTACAGCTC 
TCGCTCCAGC TGGCTCATTA CAAGGACATG GGTAAATTTT CCCTCACCTA CGAGGCCTCC 
ATGACCCGGC TGTTCCGAGA GGGCAGAACG GAGACCGTGC GTTCCTGCAC CGCTGAGTCG 
TGCAGCTTCG TGCTGGCCAT GGTAGACCCC ACACAGACGG TTGAACAGAA GCTCAAGTTG 
TTCAGGATCG CGTCTGAGAA GCATCAGCTT CTGTACCGCC TGGCGATGAC CGGTGCGGGG 
ATCGACCGCC ACCTCTTCTG CCTCTACGTG GTGTCCAAGT ACCTCGCGGT CGACTCCCCT 
TTCCTGAAGG AGGTCTTAGC TGAGCCTTGG AGATTATCCA CCAGCCAGAC CCCTCCACAG 
CAGGTGGAAC TGTTCAATTT GGATAGGAAT CCAGACTACG TGTCGTGCGG AGGGGGCTTC 
GGGCCGGTTG CTGATGACGG CTATGGGGTG TCGTACATTC TCGTCGGTGA GGACCTCATC 
AATTTCCATA TATCCTCCAA GTTCTCCTGT CCTGAGACGG ATTCGCATCG CTTCGGGAAG 
AACCTGAGAG AAGCAATGAC CGACATCATC AGTTTGTTCG GGTTCAGCCC CAATTCCAAA 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010819571.1
CDS	110..1975
Translation	MVKIFSGRKPMLYSFQTSLPRLPVPAVQDTVNRYLESVKPLMKEGDFTRMTALAQDFAVNLGPRLQWYLKLKSWWATNYVSDWWEEYIYLRGRGPLMVNSNYFAMDLLYIIPTHIQAARAGNGIHAILLYRRKLDREEIKPILLLGSTIPLCSAQWERMFNTSRIPGEETDTIQHLRDSKHIVVFHRGRYFKVWLYHDGRLLRPREIEQQVQRILEDPSEPQPGEAKLAALTAADRVPWARCRQAYFGHGKNKQSLDAVEKAAFFVTLDETEQGYREEDPETSMDSYAKSLLHGRCFDRWFDKSFTFIVFKNGKMGINAEHSWADAPIMGHLWEYVMATDSFQLGYTEDGHCKGDTNPNIPYPTRLQWDIPEECQEVIETSLSSANLLANDVDFHSFPFRTFGKGLIKKCKTSPDAFVQLSLQLAHYKDMGKFSLTYEASMTRLFREGRTETVRSCTAESCSFVLAMVDPTQTVEQKLKLFRIASEKHQLLYRLAMTGAGIDRHLFCLYVVSKYLAVDSPFLKEVLAEPWRLSTSQTPPQQVELFNLDRNPDYVSCGGGFGPVADDGYGVSYILVGEDLINFHISSKFSCPETDSHRFGKNLREAMTDIISLFGFSPNSKK

Target ORF information:

RefSeq Version	XM_010821269.2
Organism	Bos taurus(cattle)
Definition	Bos taurus carnitine palmitoyltransferase 1A (liver) (CPT1A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010821269.2

ORF Insert Sequence:

ATGGTCAAGA TCTTCTCAGG CCGAAAACCC ATGTTGTACA GCTTCCAGAC GTCTCTGCCG 
CGCCTGCCCG TGCCAGCTGT CCAGGACACC GTCAACAGGT ACTTGGAATC TGTGAAGCCT 
CTTATGAAGG AGGGAGACTT TACACGGATG ACGGCTCTGG CACAAGATTT TGCTGTCAAT 
CTGGGACCAA GATTGCAGTG GTATTTGAAG CTAAAATCCT GGTGGGCTAC CAATTATGTG 
AGCGACTGGT GGGAGGAATA CATCTACCTC CGAGGACGAG GGCCGCTGAT GGTGAACAGC 
AACTACTTCG CGATGGACTT GCTGTATATC ATACCGACTC ACATCCAGGC GGCAAGAGCT 
GGCAACGGGA TCCACGCCAT CCTGCTTTAC AGACGCAAAC TGGACCGGGA GGAAATCAAA 
CCGATTCTTC TTCTGGGGTC TACGATTCCG CTCTGCTCCG CCCAGTGGGA GCGCATGTTT 
AATACTTCTC GGATCCCGGG AGAGGAGACA GACACCATCC AGCACCTGAG GGACAGCAAG 
CACATCGTCG TCTTCCACCG GGGCCGCTAC TTCAAGGTCT GGCTCTACCA CGACGGCCGG 
CTGCTGAGGC CCCGCGAGAT CGAGCAGCAG GTGCAGCGGA TCCTGGAGGA CCCGTCGGAG 
CCGCAGCCCG GGGAGGCCAA GCTGGCGGCG CTTACGGCGG CGGACAGGGT CCCCTGGGCC 
AGGTGTCGCC AGGCCTATTT TGGACACGGG AAAAACAAGC AGTCTCTGGA TGCCGTGGAA 
AAAGCAGCGT TCTTCGTGAC GTTAGACGAA ACCGAGCAGG GATACAGGGA GGAGGACCCG 
GAAACGTCGA TGGACAGCTA CGCCAAGTCC CTGCTGCATG GCAGGTGTTT CGACAGGTGG 
TTTGATAAAT CATTCACGTT TATCGTCTTT AAAAACGGAA AGATGGGCAT TAATGCGGAG 
CACTCCTGGG CCGATGCGCC CATCATGGGT CACCTTTGGG AGTACGTCAT GGCCACAGAC 
AGCTTCCAGC TGGGTTACAC GGAGGATGGA CACTGTAAAG GAGACACCAA CCCGAACATC 
CCGTACCCTA CCCGGCTGCA GTGGGACATC CCGGAGGAAT GTCAGGAGGT CATTGAGACT 
TCACTGAGCA GTGCCAACCT CCTGGCCAAC GACGTGGATT TCCACTCCTT CCCATTCCGC 
ACTTTCGGTA AAGGGCTGAT TAAGAAATGT AAAACCAGCC CGGACGCCTT CGTACAGCTC 
TCGCTCCAGC TGGCTCATTA CAAGGACATG GGTAAATTTT CCCTCACCTA CGAGGCCTCC 
ATGACCCGGC TGTTCCGAGA GGGCAGAACG GAGACCGTGC GTTCCTGCAC CGCTGAGTCG 
TGCAGCTTCG TGCTGGCCAT GGTAGACCCC ACACAGACGG TTGAACAGAA GCTCAAGTTG 
TTCAGGATCG CGTCTGAGAA GCATCAGCTT CTGTACCGCC TGGCGATGAC CGGTGCGGGG 
ATCGACCGCC ACCTCTTCTG CCTCTACGTG GTGTCCAAGT ACCTCGCGGT CGACTCCCCT 
TTCCTGAAGG AGGTCTTAGC TGAGCCTTGG AGATTATCCA CCAGCCAGAC CCCTCCACAG 
CAGGTGGAAC TGTTCAATTT GGATAGGAAT CCAGACTACG TGTCGTGCGG AGGGGGCTTC 
GGGCCGGTTG CTGATGACGG CTATGGGGTG TCGTACATTC TCGTCGGTGA GGACCTCATC 
AATTTCCATA TATCCTCCAA GTTCTCCTGT CCTGAGACGG ATTCGCATCG CTTCGGGAAG 
AACCTGAGAG AAGCAATGAC CGACATCATC AGTTTGTTCG GGTTCAGCCC CAATTCCAAA 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CPT1A ( NM_001304989.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001291918.1 Bos taurus carnitine palmitoyltransferase 1A (CPT1A), mRNA.

Price & Availability↑

CloneID	OBa35037
Clone ID Related Accession (Same CDS sequence)	NM_001304989.1 , XM_005227376.3
Accession Version	NM_001304989.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-22
Organism	Bos taurus(cattle)
Product	carnitine O-palmitoyltransferase 1, liver isoform
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02063651.1 and DAAA02063650.1. On or before Feb 12, 2015 this sequence version replaced XM_002699420.4, XM_001789518.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00120237, SAMN00120238 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGCGGAGG CCCACCAAGC CGTGGCCTTC CAGTTCACAG TCACTCCAGA TGGGATCGAC 
CTCCGGATGA GCCATGAGGC CCTCAGGCAG ATCTATCTGT CTGGTCTTCA TTCCTGGAAG 
AAGAAGTTCA TCAGATTCAA GAATGGCATC ATCACCGGCG TGTACCCAGC GAGTCCCTCC 
AGTTGGCTCA TCGTGGTTGT GGGCGTGATG TCGACCATGT ACGCCAAGAT CGACCCCTCT 
TTGGGAATAA TCGCTAAGAT CAACCGGACC CTGGACACGA CTGGCTGCAT GTCCAGCCAG 
ACCAAGAAGG TGGTGAGCGG CGTCATCTTC GGCACGGGGC TGTGGGTGGC CCTCATCGTC 
ACCATGCGTT ACTCCCTGAA GGTGCTGCTG TCCTACCACG GCTGGATGTT CACCCAGCAC 
GGCAGGTTGA GCCGCACCAC CAAGATCTGG ATGTTTATGG TCAAGATCTT CTCAGGCCGA 
AAACCCATGT TGTACAGCTT CCAGACGTCT CTGCCGCGCC TGCCCGTGCC AGCTGTCCAG 
GACACCGTCA ACAGGTACTT GGAATCTGTG AAGCCTCTTA TGAAGGAGGG AGACTTTACA 
CGGATGACGG CTCTGGCACA AGATTTTGCT GTCAATCTGG GACCAAGATT GCAGTGGTAT 
TTGAAGCTAA AATCCTGGTG GGCTACCAAT TATGTGAGCG ACTGGTGGGA GGAATACATC 
TACCTCCGAG GACGAGGGCC GCTGATGGTG AACAGCAACT ACTTCGCGAT GGACTTGCTG 
TATATCATAC CGACTCACAT CCAGGCGGCA AGAGCTGGCA ACGGGATCCA CGCCATCCTG 
CTTTACAGAC GCAAACTGGA CCGGGAGGAA ATCAAACCGA TTCTTCTTCT GGGGTCTACG 
ATTCCGCTCT GCTCCGCCCA GTGGGAGCGC ATGTTTAATA CTTCTCGGAT CCCGGGAGAG 
GAGACAGACA CCATCCAGCA CCTGAGGGAC AGCAAGCACA TCGTCGTCTT CCACCGGGGC 
CGCTACTTCA AGGTCTGGCT CTACCACGAC GGCCGGCTGC TGAGGCCCCG CGAGATCGAG 
CAGCAGGTGC AGCGGATCCT GGAGGACCCG TCGGAGCCGC AGCCCGGGGA GGCCAAGCTG 
GCGGCGCTTA CGGCGGCGGA CAGGGTCCCC TGGGCCAGGT GTCGCCAGGC CTATTTTGGA 
CACGGGAAAA ACAAGCAGTC TCTGGATGCC GTGGAAAAAG CAGCGTTCTT CGTGACGTTA 
GACGAAACCG AGCAGGGATA CAGGGAGGAG GACCCGGAAA CGTCGATGGA CAGCTACGCC 
AAGTCCCTGC TGCATGGCAG GTGTTTCGAC AGGTGGTTTG ATAAATCATT CACGTTTATC 
GTCTTTAAAA ACGGAAAGAT GGGCATTAAT GCGGAGCACT CCTGGGCCGA TGCGCCCATC 
ATGGGTCACC TTTGGGAGTA CGTCATGGCC ACAGACAGCT TCCAGCTGGG TTACACGGAG 
GATGGACACT GTAAAGGAGA CACCAACCCG AACATCCCGT ACCCTACCCG GCTGCAGTGG 
GACATCCCGG AGGAATGTCA GGAGGTCATT GAGACTTCAC TGAGCAGTGC CAACCTCCTG 
GCCAACGACG TGGATTTCCA CTCCTTCCCA TTCCGCACTT TCGGTAAAGG GCTGATTAAG 
AAATGTAAAA CCAGCCCGGA CGCCTTCGTA CAGCTCTCGC TCCAGCTGGC TCATTACAAG 
GACATGGGTA AATTTTCCCT CACCTACGAG GCCTCCATGA CCCGGCTGTT CCGAGAGGGC 
AGAACGGAGA CCGTGCGTTC CTGCACCGCT GAGTCGTGCA GCTTCGTGCT GGCCATGGTA 
GACCCCACAC AGACGGTTGA ACAGAAGCTC AAGTTGTTCA GGATCGCGTC TGAGAAGCAT 
CAGCTTCTGT ACCGCCTGGC GATGACCGGT GCGGGGATCG ACCGCCACCT CTTCTGCCTC 
TACGTGGTGT CCAAGTACCT CGCGGTCGAC TCCCCTTTCC TGAAGGAGGT CTTAGCTGAG 
CCTTGGAGAT TATCCACCAG CCAGACCCCT CCACAGCAGG TGGAACTGTT CAATTTGGAT 
AGGAATCCAG ACTACGTGTC GTGCGGAGGG GGCTTCGGGC CGGTTGCTGA TGACGGCTAT 
GGGGTGTCGT ACATTCTCGT CGGTGAGGAC CTCATCAATT TCCATATATC CTCCAAGTTC 
TCCTGTCCTG AGACGGATTC GCATCGCTTC GGGAAGAACC TGAGAGAAGC AATGACCGAC 
ATCATCAGTT TGTTCGGGTT CAGCCCCAAT TCCAAAAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001291918.1
CDS	140..2461
Translation	MAEAHQAVAFQFTVTPDGIDLRMSHEALRQIYLSGLHSWKKKFIRFKNGIITGVYPASPSSWLIVVVGVMSTMYAKIDPSLGIIAKINRTLDTTGCMSSQTKKVVSGVIFGTGLWVALIVTMRYSLKVLLSYHGWMFTQHGRLSRTTKIWMFMVKIFSGRKPMLYSFQTSLPRLPVPAVQDTVNRYLESVKPLMKEGDFTRMTALAQDFAVNLGPRLQWYLKLKSWWATNYVSDWWEEYIYLRGRGPLMVNSNYFAMDLLYIIPTHIQAARAGNGIHAILLYRRKLDREEIKPILLLGSTIPLCSAQWERMFNTSRIPGEETDTIQHLRDSKHIVVFHRGRYFKVWLYHDGRLLRPREIEQQVQRILEDPSEPQPGEAKLAALTAADRVPWARCRQAYFGHGKNKQSLDAVEKAAFFVTLDETEQGYREEDPETSMDSYAKSLLHGRCFDRWFDKSFTFIVFKNGKMGINAEHSWADAPIMGHLWEYVMATDSFQLGYTEDGHCKGDTNPNIPYPTRLQWDIPEECQEVIETSLSSANLLANDVDFHSFPFRTFGKGLIKKCKTSPDAFVQLSLQLAHYKDMGKFSLTYEASMTRLFREGRTETVRSCTAESCSFVLAMVDPTQTVEQKLKLFRIASEKHQLLYRLAMTGAGIDRHLFCLYVVSKYLAVDSPFLKEVLAEPWRLSTSQTPPQQVELFNLDRNPDYVSCGGGFGPVADDGYGVSYILVGEDLINFHISSKFSCPETDSHRFGKNLREAMTDIISLFGFSPNSKK

Target ORF information:

RefSeq Version	NM_001304989.1
Organism	Bos taurus(cattle)
Definition	Bos taurus carnitine palmitoyltransferase 1A (CPT1A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001304989.1

ORF Insert Sequence:

ATGGCGGAGG CCCACCAAGC CGTGGCCTTC CAGTTCACAG TCACTCCAGA TGGGATCGAC 
CTCCGGATGA GCCATGAGGC CCTCAGGCAG ATCTATCTGT CTGGTCTTCA TTCCTGGAAG 
AAGAAGTTCA TCAGATTCAA GAATGGCATC ATCACCGGCG TGTACCCAGC GAGTCCCTCC 
AGTTGGCTCA TCGTGGTTGT GGGCGTGATG TCGACCATGT ACGCCAAGAT CGACCCCTCT 
TTGGGAATAA TCGCTAAGAT CAACCGGACC CTGGACACGA CTGGCTGCAT GTCCAGCCAG 
ACCAAGAAGG TGGTGAGCGG CGTCATCTTC GGCACGGGGC TGTGGGTGGC CCTCATCGTC 
ACCATGCGTT ACTCCCTGAA GGTGCTGCTG TCCTACCACG GCTGGATGTT CACCCAGCAC 
GGCAGGTTGA GCCGCACCAC CAAGATCTGG ATGTTTATGG TCAAGATCTT CTCAGGCCGA 
AAACCCATGT TGTACAGCTT CCAGACGTCT CTGCCGCGCC TGCCCGTGCC AGCTGTCCAG 
GACACCGTCA ACAGGTACTT GGAATCTGTG AAGCCTCTTA TGAAGGAGGG AGACTTTACA 
CGGATGACGG CTCTGGCACA AGATTTTGCT GTCAATCTGG GACCAAGATT GCAGTGGTAT 
TTGAAGCTAA AATCCTGGTG GGCTACCAAT TATGTGAGCG ACTGGTGGGA GGAATACATC 
TACCTCCGAG GACGAGGGCC GCTGATGGTG AACAGCAACT ACTTCGCGAT GGACTTGCTG 
TATATCATAC CGACTCACAT CCAGGCGGCA AGAGCTGGCA ACGGGATCCA CGCCATCCTG 
CTTTACAGAC GCAAACTGGA CCGGGAGGAA ATCAAACCGA TTCTTCTTCT GGGGTCTACG 
ATTCCGCTCT GCTCCGCCCA GTGGGAGCGC ATGTTTAATA CTTCTCGGAT CCCGGGAGAG 
GAGACAGACA CCATCCAGCA CCTGAGGGAC AGCAAGCACA TCGTCGTCTT CCACCGGGGC 
CGCTACTTCA AGGTCTGGCT CTACCACGAC GGCCGGCTGC TGAGGCCCCG CGAGATCGAG 
CAGCAGGTGC AGCGGATCCT GGAGGACCCG TCGGAGCCGC AGCCCGGGGA GGCCAAGCTG 
GCGGCGCTTA CGGCGGCGGA CAGGGTCCCC TGGGCCAGGT GTCGCCAGGC CTATTTTGGA 
CACGGGAAAA ACAAGCAGTC TCTGGATGCC GTGGAAAAAG CAGCGTTCTT CGTGACGTTA 
GACGAAACCG AGCAGGGATA CAGGGAGGAG GACCCGGAAA CGTCGATGGA CAGCTACGCC 
AAGTCCCTGC TGCATGGCAG GTGTTTCGAC AGGTGGTTTG ATAAATCATT CACGTTTATC 
GTCTTTAAAA ACGGAAAGAT GGGCATTAAT GCGGAGCACT CCTGGGCCGA TGCGCCCATC 
ATGGGTCACC TTTGGGAGTA CGTCATGGCC ACAGACAGCT TCCAGCTGGG TTACACGGAG 
GATGGACACT GTAAAGGAGA CACCAACCCG AACATCCCGT ACCCTACCCG GCTGCAGTGG 
GACATCCCGG AGGAATGTCA GGAGGTCATT GAGACTTCAC TGAGCAGTGC CAACCTCCTG 
GCCAACGACG TGGATTTCCA CTCCTTCCCA TTCCGCACTT TCGGTAAAGG GCTGATTAAG 
AAATGTAAAA CCAGCCCGGA CGCCTTCGTA CAGCTCTCGC TCCAGCTGGC TCATTACAAG 
GACATGGGTA AATTTTCCCT CACCTACGAG GCCTCCATGA CCCGGCTGTT CCGAGAGGGC 
AGAACGGAGA CCGTGCGTTC CTGCACCGCT GAGTCGTGCA GCTTCGTGCT GGCCATGGTA 
GACCCCACAC AGACGGTTGA ACAGAAGCTC AAGTTGTTCA GGATCGCGTC TGAGAAGCAT 
CAGCTTCTGT ACCGCCTGGC GATGACCGGT GCGGGGATCG ACCGCCACCT CTTCTGCCTC 
TACGTGGTGT CCAAGTACCT CGCGGTCGAC TCCCCTTTCC TGAAGGAGGT CTTAGCTGAG 
CCTTGGAGAT TATCCACCAG CCAGACCCCT CCACAGCAGG TGGAACTGTT CAATTTGGAT 
AGGAATCCAG ACTACGTGTC GTGCGGAGGG GGCTTCGGGC CGGTTGCTGA TGACGGCTAT 
GGGGTGTCGT ACATTCTCGT CGGTGAGGAC CTCATCAATT TCCATATATC CTCCAAGTTC 
TCCTGTCCTG AGACGGATTC GCATCGCTTC GGGAAGAACC TGAGAGAAGC AATGACCGAC 
ATCATCAGTT TGTTCGGGTT CAGCCCCAAT TCCAAAAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CPT1A ( XM_005227376.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005227433.1 Bos taurus carnitine palmitoyltransferase 1A (liver) (CPT1A), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa35037
Clone ID Related Accession (Same CDS sequence)	NM_001304989.1 , XM_005227376.3
Accession Version	XM_005227376.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	carnitine O-palmitoyltransferase 1, liver isoform isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104648.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005227376.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGG CCCACCAAGC CGTGGCCTTC CAGTTCACAG TCACTCCAGA TGGGATCGAC 
CTCCGGATGA GCCATGAGGC CCTCAGGCAG ATCTATCTGT CTGGTCTTCA TTCCTGGAAG 
AAGAAGTTCA TCAGATTCAA GAATGGCATC ATCACCGGCG TGTACCCAGC GAGTCCCTCC 
AGTTGGCTCA TCGTGGTTGT GGGCGTGATG TCGACCATGT ACGCCAAGAT CGACCCCTCT 
TTGGGAATAA TCGCTAAGAT CAACCGGACC CTGGACACGA CTGGCTGCAT GTCCAGCCAG 
ACCAAGAAGG TGGTGAGCGG CGTCATCTTC GGCACGGGGC TGTGGGTGGC CCTCATCGTC 
ACCATGCGTT ACTCCCTGAA GGTGCTGCTG TCCTACCACG GCTGGATGTT CACCCAGCAC 
GGCAGGTTGA GCCGCACCAC CAAGATCTGG ATGTTTATGG TCAAGATCTT CTCAGGCCGA 
AAACCCATGT TGTACAGCTT CCAGACGTCT CTGCCGCGCC TGCCCGTGCC AGCTGTCCAG 
GACACCGTCA ACAGGTACTT GGAATCTGTG AAGCCTCTTA TGAAGGAGGG AGACTTTACA 
CGGATGACGG CTCTGGCACA AGATTTTGCT GTCAATCTGG GACCAAGATT GCAGTGGTAT 
TTGAAGCTAA AATCCTGGTG GGCTACCAAT TATGTGAGCG ACTGGTGGGA GGAATACATC 
TACCTCCGAG GACGAGGGCC GCTGATGGTG AACAGCAACT ACTTCGCGAT GGACTTGCTG 
TATATCATAC CGACTCACAT CCAGGCGGCA AGAGCTGGCA ACGGGATCCA CGCCATCCTG 
CTTTACAGAC GCAAACTGGA CCGGGAGGAA ATCAAACCGA TTCTTCTTCT GGGGTCTACG 
ATTCCGCTCT GCTCCGCCCA GTGGGAGCGC ATGTTTAATA CTTCTCGGAT CCCGGGAGAG 
GAGACAGACA CCATCCAGCA CCTGAGGGAC AGCAAGCACA TCGTCGTCTT CCACCGGGGC 
CGCTACTTCA AGGTCTGGCT CTACCACGAC GGCCGGCTGC TGAGGCCCCG CGAGATCGAG 
CAGCAGGTGC AGCGGATCCT GGAGGACCCG TCGGAGCCGC AGCCCGGGGA GGCCAAGCTG 
GCGGCGCTTA CGGCGGCGGA CAGGGTCCCC TGGGCCAGGT GTCGCCAGGC CTATTTTGGA 
CACGGGAAAA ACAAGCAGTC TCTGGATGCC GTGGAAAAAG CAGCGTTCTT CGTGACGTTA 
GACGAAACCG AGCAGGGATA CAGGGAGGAG GACCCGGAAA CGTCGATGGA CAGCTACGCC 
AAGTCCCTGC TGCATGGCAG GTGTTTCGAC AGGTGGTTTG ATAAATCATT CACGTTTATC 
GTCTTTAAAA ACGGAAAGAT GGGCATTAAT GCGGAGCACT CCTGGGCCGA TGCGCCCATC 
ATGGGTCACC TTTGGGAGTA CGTCATGGCC ACAGACAGCT TCCAGCTGGG TTACACGGAG 
GATGGACACT GTAAAGGAGA CACCAACCCG AACATCCCGT ACCCTACCCG GCTGCAGTGG 
GACATCCCGG AGGAATGTCA GGAGGTCATT GAGACTTCAC TGAGCAGTGC CAACCTCCTG 
GCCAACGACG TGGATTTCCA CTCCTTCCCA TTCCGCACTT TCGGTAAAGG GCTGATTAAG 
AAATGTAAAA CCAGCCCGGA CGCCTTCGTA CAGCTCTCGC TCCAGCTGGC TCATTACAAG 
GACATGGGTA AATTTTCCCT CACCTACGAG GCCTCCATGA CCCGGCTGTT CCGAGAGGGC 
AGAACGGAGA CCGTGCGTTC CTGCACCGCT GAGTCGTGCA GCTTCGTGCT GGCCATGGTA 
GACCCCACAC AGACGGTTGA ACAGAAGCTC AAGTTGTTCA GGATCGCGTC TGAGAAGCAT 
CAGCTTCTGT ACCGCCTGGC GATGACCGGT GCGGGGATCG ACCGCCACCT CTTCTGCCTC 
TACGTGGTGT CCAAGTACCT CGCGGTCGAC TCCCCTTTCC TGAAGGAGGT CTTAGCTGAG 
CCTTGGAGAT TATCCACCAG CCAGACCCCT CCACAGCAGG TGGAACTGTT CAATTTGGAT 
AGGAATCCAG ACTACGTGTC GTGCGGAGGG GGCTTCGGGC CGGTTGCTGA TGACGGCTAT 
GGGGTGTCGT ACATTCTCGT CGGTGAGGAC CTCATCAATT TCCATATATC CTCCAAGTTC 
TCCTGTCCTG AGACGGATTC GCATCGCTTC GGGAAGAACC TGAGAGAAGC AATGACCGAC 
ATCATCAGTT TGTTCGGGTT CAGCCCCAAT TCCAAAAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005227433.1
CDS	138..2459
Translation	MAEAHQAVAFQFTVTPDGIDLRMSHEALRQIYLSGLHSWKKKFIRFKNGIITGVYPASPSSWLIVVVGVMSTMYAKIDPSLGIIAKINRTLDTTGCMSSQTKKVVSGVIFGTGLWVALIVTMRYSLKVLLSYHGWMFTQHGRLSRTTKIWMFMVKIFSGRKPMLYSFQTSLPRLPVPAVQDTVNRYLESVKPLMKEGDFTRMTALAQDFAVNLGPRLQWYLKLKSWWATNYVSDWWEEYIYLRGRGPLMVNSNYFAMDLLYIIPTHIQAARAGNGIHAILLYRRKLDREEIKPILLLGSTIPLCSAQWERMFNTSRIPGEETDTIQHLRDSKHIVVFHRGRYFKVWLYHDGRLLRPREIEQQVQRILEDPSEPQPGEAKLAALTAADRVPWARCRQAYFGHGKNKQSLDAVEKAAFFVTLDETEQGYREEDPETSMDSYAKSLLHGRCFDRWFDKSFTFIVFKNGKMGINAEHSWADAPIMGHLWEYVMATDSFQLGYTEDGHCKGDTNPNIPYPTRLQWDIPEECQEVIETSLSSANLLANDVDFHSFPFRTFGKGLIKKCKTSPDAFVQLSLQLAHYKDMGKFSLTYEASMTRLFREGRTETVRSCTAESCSFVLAMVDPTQTVEQKLKLFRIASEKHQLLYRLAMTGAGIDRHLFCLYVVSKYLAVDSPFLKEVLAEPWRLSTSQTPPQQVELFNLDRNPDYVSCGGGFGPVADDGYGVSYILVGEDLINFHISSKFSCPETDSHRFGKNLREAMTDIISLFGFSPNSKK

Target ORF information:

RefSeq Version	XM_005227376.3
Organism	Bos taurus(cattle)
Definition	Bos taurus carnitine palmitoyltransferase 1A (liver) (CPT1A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005227376.3

ORF Insert Sequence:

ATGGCGGAGG CCCACCAAGC CGTGGCCTTC CAGTTCACAG TCACTCCAGA TGGGATCGAC 
CTCCGGATGA GCCATGAGGC CCTCAGGCAG ATCTATCTGT CTGGTCTTCA TTCCTGGAAG 
AAGAAGTTCA TCAGATTCAA GAATGGCATC ATCACCGGCG TGTACCCAGC GAGTCCCTCC 
AGTTGGCTCA TCGTGGTTGT GGGCGTGATG TCGACCATGT ACGCCAAGAT CGACCCCTCT 
TTGGGAATAA TCGCTAAGAT CAACCGGACC CTGGACACGA CTGGCTGCAT GTCCAGCCAG 
ACCAAGAAGG TGGTGAGCGG CGTCATCTTC GGCACGGGGC TGTGGGTGGC CCTCATCGTC 
ACCATGCGTT ACTCCCTGAA GGTGCTGCTG TCCTACCACG GCTGGATGTT CACCCAGCAC 
GGCAGGTTGA GCCGCACCAC CAAGATCTGG ATGTTTATGG TCAAGATCTT CTCAGGCCGA 
AAACCCATGT TGTACAGCTT CCAGACGTCT CTGCCGCGCC TGCCCGTGCC AGCTGTCCAG 
GACACCGTCA ACAGGTACTT GGAATCTGTG AAGCCTCTTA TGAAGGAGGG AGACTTTACA 
CGGATGACGG CTCTGGCACA AGATTTTGCT GTCAATCTGG GACCAAGATT GCAGTGGTAT 
TTGAAGCTAA AATCCTGGTG GGCTACCAAT TATGTGAGCG ACTGGTGGGA GGAATACATC 
TACCTCCGAG GACGAGGGCC GCTGATGGTG AACAGCAACT ACTTCGCGAT GGACTTGCTG 
TATATCATAC CGACTCACAT CCAGGCGGCA AGAGCTGGCA ACGGGATCCA CGCCATCCTG 
CTTTACAGAC GCAAACTGGA CCGGGAGGAA ATCAAACCGA TTCTTCTTCT GGGGTCTACG 
ATTCCGCTCT GCTCCGCCCA GTGGGAGCGC ATGTTTAATA CTTCTCGGAT CCCGGGAGAG 
GAGACAGACA CCATCCAGCA CCTGAGGGAC AGCAAGCACA TCGTCGTCTT CCACCGGGGC 
CGCTACTTCA AGGTCTGGCT CTACCACGAC GGCCGGCTGC TGAGGCCCCG CGAGATCGAG 
CAGCAGGTGC AGCGGATCCT GGAGGACCCG TCGGAGCCGC AGCCCGGGGA GGCCAAGCTG 
GCGGCGCTTA CGGCGGCGGA CAGGGTCCCC TGGGCCAGGT GTCGCCAGGC CTATTTTGGA 
CACGGGAAAA ACAAGCAGTC TCTGGATGCC GTGGAAAAAG CAGCGTTCTT CGTGACGTTA 
GACGAAACCG AGCAGGGATA CAGGGAGGAG GACCCGGAAA CGTCGATGGA CAGCTACGCC 
AAGTCCCTGC TGCATGGCAG GTGTTTCGAC AGGTGGTTTG ATAAATCATT CACGTTTATC 
GTCTTTAAAA ACGGAAAGAT GGGCATTAAT GCGGAGCACT CCTGGGCCGA TGCGCCCATC 
ATGGGTCACC TTTGGGAGTA CGTCATGGCC ACAGACAGCT TCCAGCTGGG TTACACGGAG 
GATGGACACT GTAAAGGAGA CACCAACCCG AACATCCCGT ACCCTACCCG GCTGCAGTGG 
GACATCCCGG AGGAATGTCA GGAGGTCATT GAGACTTCAC TGAGCAGTGC CAACCTCCTG 
GCCAACGACG TGGATTTCCA CTCCTTCCCA TTCCGCACTT TCGGTAAAGG GCTGATTAAG 
AAATGTAAAA CCAGCCCGGA CGCCTTCGTA CAGCTCTCGC TCCAGCTGGC TCATTACAAG 
GACATGGGTA AATTTTCCCT CACCTACGAG GCCTCCATGA CCCGGCTGTT CCGAGAGGGC 
AGAACGGAGA CCGTGCGTTC CTGCACCGCT GAGTCGTGCA GCTTCGTGCT GGCCATGGTA 
GACCCCACAC AGACGGTTGA ACAGAAGCTC AAGTTGTTCA GGATCGCGTC TGAGAAGCAT 
CAGCTTCTGT ACCGCCTGGC GATGACCGGT GCGGGGATCG ACCGCCACCT CTTCTGCCTC 
TACGTGGTGT CCAAGTACCT CGCGGTCGAC TCCCCTTTCC TGAAGGAGGT CTTAGCTGAG 
CCTTGGAGAT TATCCACCAG CCAGACCCCT CCACAGCAGG TGGAACTGTT CAATTTGGAT 
AGGAATCCAG ACTACGTGTC GTGCGGAGGG GGCTTCGGGC CGGTTGCTGA TGACGGCTAT 
GGGGTGTCGT ACATTCTCGT CGGTGAGGAC CTCATCAATT TCCATATATC CTCCAAGTTC 
TCCTGTCCTG AGACGGATTC GCATCGCTTC GGGAAGAACC TGAGAGAAGC AATGACCGAC 
ATCATCAGTT TGTTCGGGTT CAGCCCCAAT TCCAAAAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CPT1A ( NM_001304989.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001291918.1 Bos taurus carnitine palmitoyltransferase 1A (CPT1A), mRNA.

Price & Availability↑

CloneID	OBa231884
Clone ID Related Accession (Same CDS sequence)	NM_001304989.2 , XM_005227376.4
Accession Version	NM_001304989.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-28
Organism	Bos taurus(cattle)
Product	carnitine O-palmitoyltransferase 1, liver isoform
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000029.1. On May 29, 2018 this sequence version replaced NM_001304989.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5721697.1051.1, SRR5571257.53716.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGCGGAGG CCCACCAAGC CGTGGCCTTC CAGTTCACAG TCACTCCAGA TGGGATCGAC 
CTCCGGATGA GCCATGAGGC CCTCAGGCAG ATCTATCTGT CTGGTCTTCA TTCCTGGAAG 
AAGAAGTTCA TCAGATTCAA GAATGGCATC ATCACCGGCG TGTACCCAGC GAGTCCCTCC 
AGTTGGCTCA TCGTGGTTGT GGGCGTGATG TCGACCATGT ACGCCAAGAT CGACCCCTCT 
TTGGGAATAA TCGCTAAGAT CAATCGGACC CTGGACACGA CTGGCTGCAT GTCCAGCCAG 
ACCAAGAAGG TGGTGAGCGG CGTCATCTTC GGCACGGGGC TGTGGGTGGC CCTCATCGTC 
ACCATGCGTT ACTCCCTGAA GGTGCTGCTG TCCTACCACG GCTGGATGTT CACCCAGCAC 
GGCAGGTTGA GCCGCACCAC CAAGATCTGG ATGTTTATGG TCAAGATCTT CTCAGGCCGA 
AAACCCATGT TGTACAGCTT CCAGACGTCT CTGCCGCGCC TGCCCGTGCC AGCTGTCCAG 
GACACCGTCA ACAGGTACTT GGAATCTGTG AAGCCTCTTA TGAAGGAGGG AGACTTTACA 
CGGATGACGG CTCTGGCACA AGATTTTGCT GTCAATCTGG GACCAAGATT GCAGTGGTAT 
TTGAAGCTAA AATCCTGGTG GGCTACCAAT TATGTGAGCG ACTGGTGGGA GGAATACATC 
TACCTCCGAG GACGAGGGCC GCTGATGGTG AACAGCAACT ACTTCGCGAT GGACTTGCTG 
TATATCATAC CGACTCACAT CCAGGCGGCA AGAGCTGGCA ACGGGATCCA CGCCATCCTG 
CTTTACAGAC GCAAACTGGA CCGGGAGGAA ATCAAACCGA TTCTTCTTCT GGGGTCTACG 
ATTCCGCTCT GCTCCGCCCA GTGGGAGCGC ATGTTTAATA CTTCTCGGAT CCCGGGAGAG 
GAGACAGACA CCATCCAGCA CCTGAGGGAC AGCAAGCACA TCGTCGTCTT CCACCGGGGC 
CGCTACTTCA AGGTCTGGCT CTACCACGAC GGCCGGCTGC TGAGGCCCCG CGAGATCGAG 
CAGCAGGTGC AGCGGATCCT GGAGGACCCG TCGGAGCCGC AGCCCGGGGA GGCCAAGCTG 
GCGGCGCTTA CGGCGGCGGA CAGGGTCCCC TGGGCCAGGT GTCGCCAGGC CTATTTTGGA 
CACGGGAAAA ACAAGCAGTC TCTGGATGCC GTGGAAAAAG CAGCGTTCTT CGTGACGTTA 
GACGAAACCG AGCAGGGATA CAGGGAGGAG GACCCGGAAA CGTCGATGGA CAGCTACGCC 
AAGTCCCTGC TGCATGGCAG GTGTTTCGAC AGGTGGTTTG ATAAATCATT CACGTTTATC 
GTCTTTAAAA ACGGAAAGAT GGGCATTAAT GCGGAGCACT CCTGGGCCGA TGCGCCCATC 
ATGGGTCACC TTTGGGAGTA CGTCATGGCC ACAGACAGCT TCCAGCTGGG TTACACGGAG 
GATGGACACT GTAAAGGAGA CACCAACCCG AACATCCCGT ACCCTACCCG GCTGCAGTGG 
GACATCCCGG AGGAATGTCA GGAGGTCATT GAGACTTCGC TGAGCAGTGC CAACCTCCTG 
GCCAACGACG TGGATTTCCA CTCCTTCCCA TTCCGCACTT TCGGTAAAGG GCTGATTAAG 
AAATGTAAAA CCAGCCCGGA CGCCTTCGTA CAGCTCTCGC TCCAGCTGGC TCATTACAAG 
GACATGGGTA AATTTTCCCT CACCTACGAG GCCTCCATGA CCCGGCTGTT CCGAGAGGGC 
AGAACGGAGA CCGTGCGTTC CTGCACCGCT GAGTCGTGCA GCTTCGTGCT GGCCATGGTA 
GACCCCACAC AGACGGTTGA ACAGAAGCTC AAGTTGTTCA GGATCGCGTC TGAGAAGCAT 
CAGCTTCTGT ACCGCCTGGC GATGACCGGT GCGGGGATCG ACCGCCACCT CTTCTGCCTC 
TACGTGGTGT CCAAGTACCT CGCGGTCGAC TCCCCTTTCC TGAAGGAGGT CTTAGCTGAG 
CCTTGGAGAT TATCCACCAG CCAGACCCCT CCACAGCAGG TGGAACTGTT CAATTTGGAT 
AGGAATCCAG ACTACGTGTC GTGCGGAGGG GGCTTCGGGC CGGTTGCTGA TGACGGCTAT 
GGGGTGTCGT ACATTCTCGT CGGTGAGGAC CTCATCAATT TCCACATATC CTCCAAGTTC 
TCCTGTCCTG AGACGGATTC GCATCGCTTC GGGAAGAACC TGAGAGAAGC AATGACCGAC 
ATCATCAGTT TGTTCGGGTT CAGCCCCAAT TCCAAAAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001291918.1
CDS	140..2461
Translation	MAEAHQAVAFQFTVTPDGIDLRMSHEALRQIYLSGLHSWKKKFIRFKNGIITGVYPASPSSWLIVVVGVMSTMYAKIDPSLGIIAKINRTLDTTGCMSSQTKKVVSGVIFGTGLWVALIVTMRYSLKVLLSYHGWMFTQHGRLSRTTKIWMFMVKIFSGRKPMLYSFQTSLPRLPVPAVQDTVNRYLESVKPLMKEGDFTRMTALAQDFAVNLGPRLQWYLKLKSWWATNYVSDWWEEYIYLRGRGPLMVNSNYFAMDLLYIIPTHIQAARAGNGIHAILLYRRKLDREEIKPILLLGSTIPLCSAQWERMFNTSRIPGEETDTIQHLRDSKHIVVFHRGRYFKVWLYHDGRLLRPREIEQQVQRILEDPSEPQPGEAKLAALTAADRVPWARCRQAYFGHGKNKQSLDAVEKAAFFVTLDETEQGYREEDPETSMDSYAKSLLHGRCFDRWFDKSFTFIVFKNGKMGINAEHSWADAPIMGHLWEYVMATDSFQLGYTEDGHCKGDTNPNIPYPTRLQWDIPEECQEVIETSLSSANLLANDVDFHSFPFRTFGKGLIKKCKTSPDAFVQLSLQLAHYKDMGKFSLTYEASMTRLFREGRTETVRSCTAESCSFVLAMVDPTQTVEQKLKLFRIASEKHQLLYRLAMTGAGIDRHLFCLYVVSKYLAVDSPFLKEVLAEPWRLSTSQTPPQQVELFNLDRNPDYVSCGGGFGPVADDGYGVSYILVGEDLINFHISSKFSCPETDSHRFGKNLREAMTDIISLFGFSPNSKK

Target ORF information:

RefSeq Version	NM_001304989.2
Organism	Bos taurus(cattle)
Definition	Bos taurus carnitine palmitoyltransferase 1A (CPT1A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001304989.2

ORF Insert Sequence:

ATGGCGGAGG CCCACCAAGC CGTGGCCTTC CAGTTCACAG TCACTCCAGA TGGGATCGAC 
CTCCGGATGA GCCATGAGGC CCTCAGGCAG ATCTATCTGT CTGGTCTTCA TTCCTGGAAG 
AAGAAGTTCA TCAGATTCAA GAATGGCATC ATCACCGGCG TGTACCCAGC GAGTCCCTCC 
AGTTGGCTCA TCGTGGTTGT GGGCGTGATG TCGACCATGT ACGCCAAGAT CGACCCCTCT 
TTGGGAATAA TCGCTAAGAT CAATCGGACC CTGGACACGA CTGGCTGCAT GTCCAGCCAG 
ACCAAGAAGG TGGTGAGCGG CGTCATCTTC GGCACGGGGC TGTGGGTGGC CCTCATCGTC 
ACCATGCGTT ACTCCCTGAA GGTGCTGCTG TCCTACCACG GCTGGATGTT CACCCAGCAC 
GGCAGGTTGA GCCGCACCAC CAAGATCTGG ATGTTTATGG TCAAGATCTT CTCAGGCCGA 
AAACCCATGT TGTACAGCTT CCAGACGTCT CTGCCGCGCC TGCCCGTGCC AGCTGTCCAG 
GACACCGTCA ACAGGTACTT GGAATCTGTG AAGCCTCTTA TGAAGGAGGG AGACTTTACA 
CGGATGACGG CTCTGGCACA AGATTTTGCT GTCAATCTGG GACCAAGATT GCAGTGGTAT 
TTGAAGCTAA AATCCTGGTG GGCTACCAAT TATGTGAGCG ACTGGTGGGA GGAATACATC 
TACCTCCGAG GACGAGGGCC GCTGATGGTG AACAGCAACT ACTTCGCGAT GGACTTGCTG 
TATATCATAC CGACTCACAT CCAGGCGGCA AGAGCTGGCA ACGGGATCCA CGCCATCCTG 
CTTTACAGAC GCAAACTGGA CCGGGAGGAA ATCAAACCGA TTCTTCTTCT GGGGTCTACG 
ATTCCGCTCT GCTCCGCCCA GTGGGAGCGC ATGTTTAATA CTTCTCGGAT CCCGGGAGAG 
GAGACAGACA CCATCCAGCA CCTGAGGGAC AGCAAGCACA TCGTCGTCTT CCACCGGGGC 
CGCTACTTCA AGGTCTGGCT CTACCACGAC GGCCGGCTGC TGAGGCCCCG CGAGATCGAG 
CAGCAGGTGC AGCGGATCCT GGAGGACCCG TCGGAGCCGC AGCCCGGGGA GGCCAAGCTG 
GCGGCGCTTA CGGCGGCGGA CAGGGTCCCC TGGGCCAGGT GTCGCCAGGC CTATTTTGGA 
CACGGGAAAA ACAAGCAGTC TCTGGATGCC GTGGAAAAAG CAGCGTTCTT CGTGACGTTA 
GACGAAACCG AGCAGGGATA CAGGGAGGAG GACCCGGAAA CGTCGATGGA CAGCTACGCC 
AAGTCCCTGC TGCATGGCAG GTGTTTCGAC AGGTGGTTTG ATAAATCATT CACGTTTATC 
GTCTTTAAAA ACGGAAAGAT GGGCATTAAT GCGGAGCACT CCTGGGCCGA TGCGCCCATC 
ATGGGTCACC TTTGGGAGTA CGTCATGGCC ACAGACAGCT TCCAGCTGGG TTACACGGAG 
GATGGACACT GTAAAGGAGA CACCAACCCG AACATCCCGT ACCCTACCCG GCTGCAGTGG 
GACATCCCGG AGGAATGTCA GGAGGTCATT GAGACTTCGC TGAGCAGTGC CAACCTCCTG 
GCCAACGACG TGGATTTCCA CTCCTTCCCA TTCCGCACTT TCGGTAAAGG GCTGATTAAG 
AAATGTAAAA CCAGCCCGGA CGCCTTCGTA CAGCTCTCGC TCCAGCTGGC TCATTACAAG 
GACATGGGTA AATTTTCCCT CACCTACGAG GCCTCCATGA CCCGGCTGTT CCGAGAGGGC 
AGAACGGAGA CCGTGCGTTC CTGCACCGCT GAGTCGTGCA GCTTCGTGCT GGCCATGGTA 
GACCCCACAC AGACGGTTGA ACAGAAGCTC AAGTTGTTCA GGATCGCGTC TGAGAAGCAT 
CAGCTTCTGT ACCGCCTGGC GATGACCGGT GCGGGGATCG ACCGCCACCT CTTCTGCCTC 
TACGTGGTGT CCAAGTACCT CGCGGTCGAC TCCCCTTTCC TGAAGGAGGT CTTAGCTGAG 
CCTTGGAGAT TATCCACCAG CCAGACCCCT CCACAGCAGG TGGAACTGTT CAATTTGGAT 
AGGAATCCAG ACTACGTGTC GTGCGGAGGG GGCTTCGGGC CGGTTGCTGA TGACGGCTAT 
GGGGTGTCGT ACATTCTCGT CGGTGAGGAC CTCATCAATT TCCACATATC CTCCAAGTTC 
TCCTGTCCTG AGACGGATTC GCATCGCTTC GGGAAGAACC TGAGAGAAGC AATGACCGAC 
ATCATCAGTT TGTTCGGGTT CAGCCCCAAT TCCAAAAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CPT1A ( XM_005227376.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005227433.1 Bos taurus carnitine palmitoyltransferase 1A (CPT1A), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa231884
Clone ID Related Accession (Same CDS sequence)	NM_001304989.2 , XM_005227376.4
Accession Version	XM_005227376.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	carnitine O-palmitoyltransferase 1, liver isoform isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037356.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005227376.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGG CCCACCAAGC CGTGGCCTTC CAGTTCACAG TCACTCCAGA TGGGATCGAC 
CTCCGGATGA GCCATGAGGC CCTCAGGCAG ATCTATCTGT CTGGTCTTCA TTCCTGGAAG 
AAGAAGTTCA TCAGATTCAA GAATGGCATC ATCACCGGCG TGTACCCAGC GAGTCCCTCC 
AGTTGGCTCA TCGTGGTTGT GGGCGTGATG TCGACCATGT ACGCCAAGAT CGACCCCTCT 
TTGGGAATAA TCGCTAAGAT CAATCGGACC CTGGACACGA CTGGCTGCAT GTCCAGCCAG 
ACCAAGAAGG TGGTGAGCGG CGTCATCTTC GGCACGGGGC TGTGGGTGGC CCTCATCGTC 
ACCATGCGTT ACTCCCTGAA GGTGCTGCTG TCCTACCACG GCTGGATGTT CACCCAGCAC 
GGCAGGTTGA GCCGCACCAC CAAGATCTGG ATGTTTATGG TCAAGATCTT CTCAGGCCGA 
AAACCCATGT TGTACAGCTT CCAGACGTCT CTGCCGCGCC TGCCCGTGCC AGCTGTCCAG 
GACACCGTCA ACAGGTACTT GGAATCTGTG AAGCCTCTTA TGAAGGAGGG AGACTTTACA 
CGGATGACGG CTCTGGCACA AGATTTTGCT GTCAATCTGG GACCAAGATT GCAGTGGTAT 
TTGAAGCTAA AATCCTGGTG GGCTACCAAT TATGTGAGCG ACTGGTGGGA GGAATACATC 
TACCTCCGAG GACGAGGGCC GCTGATGGTG AACAGCAACT ACTTCGCGAT GGACTTGCTG 
TATATCATAC CGACTCACAT CCAGGCGGCA AGAGCTGGCA ACGGGATCCA CGCCATCCTG 
CTTTACAGAC GCAAACTGGA CCGGGAGGAA ATCAAACCGA TTCTTCTTCT GGGGTCTACG 
ATTCCGCTCT GCTCCGCCCA GTGGGAGCGC ATGTTTAATA CTTCTCGGAT CCCGGGAGAG 
GAGACAGACA CCATCCAGCA CCTGAGGGAC AGCAAGCACA TCGTCGTCTT CCACCGGGGC 
CGCTACTTCA AGGTCTGGCT CTACCACGAC GGCCGGCTGC TGAGGCCCCG CGAGATCGAG 
CAGCAGGTGC AGCGGATCCT GGAGGACCCG TCGGAGCCGC AGCCCGGGGA GGCCAAGCTG 
GCGGCGCTTA CGGCGGCGGA CAGGGTCCCC TGGGCCAGGT GTCGCCAGGC CTATTTTGGA 
CACGGGAAAA ACAAGCAGTC TCTGGATGCC GTGGAAAAAG CAGCGTTCTT CGTGACGTTA 
GACGAAACCG AGCAGGGATA CAGGGAGGAG GACCCGGAAA CGTCGATGGA CAGCTACGCC 
AAGTCCCTGC TGCATGGCAG GTGTTTCGAC AGGTGGTTTG ATAAATCATT CACGTTTATC 
GTCTTTAAAA ACGGAAAGAT GGGCATTAAT GCGGAGCACT CCTGGGCCGA TGCGCCCATC 
ATGGGTCACC TTTGGGAGTA CGTCATGGCC ACAGACAGCT TCCAGCTGGG TTACACGGAG 
GATGGACACT GTAAAGGAGA CACCAACCCG AACATCCCGT ACCCTACCCG GCTGCAGTGG 
GACATCCCGG AGGAATGTCA GGAGGTCATT GAGACTTCGC TGAGCAGTGC CAACCTCCTG 
GCCAACGACG TGGATTTCCA CTCCTTCCCA TTCCGCACTT TCGGTAAAGG GCTGATTAAG 
AAATGTAAAA CCAGCCCGGA CGCCTTCGTA CAGCTCTCGC TCCAGCTGGC TCATTACAAG 
GACATGGGTA AATTTTCCCT CACCTACGAG GCCTCCATGA CCCGGCTGTT CCGAGAGGGC 
AGAACGGAGA CCGTGCGTTC CTGCACCGCT GAGTCGTGCA GCTTCGTGCT GGCCATGGTA 
GACCCCACAC AGACGGTTGA ACAGAAGCTC AAGTTGTTCA GGATCGCGTC TGAGAAGCAT 
CAGCTTCTGT ACCGCCTGGC GATGACCGGT GCGGGGATCG ACCGCCACCT CTTCTGCCTC 
TACGTGGTGT CCAAGTACCT CGCGGTCGAC TCCCCTTTCC TGAAGGAGGT CTTAGCTGAG 
CCTTGGAGAT TATCCACCAG CCAGACCCCT CCACAGCAGG TGGAACTGTT CAATTTGGAT 
AGGAATCCAG ACTACGTGTC GTGCGGAGGG GGCTTCGGGC CGGTTGCTGA TGACGGCTAT 
GGGGTGTCGT ACATTCTCGT CGGTGAGGAC CTCATCAATT TCCACATATC CTCCAAGTTC 
TCCTGTCCTG AGACGGATTC GCATCGCTTC GGGAAGAACC TGAGAGAAGC AATGACCGAC 
ATCATCAGTT TGTTCGGGTT CAGCCCCAAT TCCAAAAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005227433.1
CDS	746..3067
Translation	MAEAHQAVAFQFTVTPDGIDLRMSHEALRQIYLSGLHSWKKKFIRFKNGIITGVYPASPSSWLIVVVGVMSTMYAKIDPSLGIIAKINRTLDTTGCMSSQTKKVVSGVIFGTGLWVALIVTMRYSLKVLLSYHGWMFTQHGRLSRTTKIWMFMVKIFSGRKPMLYSFQTSLPRLPVPAVQDTVNRYLESVKPLMKEGDFTRMTALAQDFAVNLGPRLQWYLKLKSWWATNYVSDWWEEYIYLRGRGPLMVNSNYFAMDLLYIIPTHIQAARAGNGIHAILLYRRKLDREEIKPILLLGSTIPLCSAQWERMFNTSRIPGEETDTIQHLRDSKHIVVFHRGRYFKVWLYHDGRLLRPREIEQQVQRILEDPSEPQPGEAKLAALTAADRVPWARCRQAYFGHGKNKQSLDAVEKAAFFVTLDETEQGYREEDPETSMDSYAKSLLHGRCFDRWFDKSFTFIVFKNGKMGINAEHSWADAPIMGHLWEYVMATDSFQLGYTEDGHCKGDTNPNIPYPTRLQWDIPEECQEVIETSLSSANLLANDVDFHSFPFRTFGKGLIKKCKTSPDAFVQLSLQLAHYKDMGKFSLTYEASMTRLFREGRTETVRSCTAESCSFVLAMVDPTQTVEQKLKLFRIASEKHQLLYRLAMTGAGIDRHLFCLYVVSKYLAVDSPFLKEVLAEPWRLSTSQTPPQQVELFNLDRNPDYVSCGGGFGPVADDGYGVSYILVGEDLINFHISSKFSCPETDSHRFGKNLREAMTDIISLFGFSPNSKK

Target ORF information:

RefSeq Version	XM_005227376.4
Organism	Bos taurus(cattle)
Definition	Bos taurus carnitine palmitoyltransferase 1A (CPT1A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005227376.4

ORF Insert Sequence:

ATGGCGGAGG CCCACCAAGC CGTGGCCTTC CAGTTCACAG TCACTCCAGA TGGGATCGAC 
CTCCGGATGA GCCATGAGGC CCTCAGGCAG ATCTATCTGT CTGGTCTTCA TTCCTGGAAG 
AAGAAGTTCA TCAGATTCAA GAATGGCATC ATCACCGGCG TGTACCCAGC GAGTCCCTCC 
AGTTGGCTCA TCGTGGTTGT GGGCGTGATG TCGACCATGT ACGCCAAGAT CGACCCCTCT 
TTGGGAATAA TCGCTAAGAT CAATCGGACC CTGGACACGA CTGGCTGCAT GTCCAGCCAG 
ACCAAGAAGG TGGTGAGCGG CGTCATCTTC GGCACGGGGC TGTGGGTGGC CCTCATCGTC 
ACCATGCGTT ACTCCCTGAA GGTGCTGCTG TCCTACCACG GCTGGATGTT CACCCAGCAC 
GGCAGGTTGA GCCGCACCAC CAAGATCTGG ATGTTTATGG TCAAGATCTT CTCAGGCCGA 
AAACCCATGT TGTACAGCTT CCAGACGTCT CTGCCGCGCC TGCCCGTGCC AGCTGTCCAG 
GACACCGTCA ACAGGTACTT GGAATCTGTG AAGCCTCTTA TGAAGGAGGG AGACTTTACA 
CGGATGACGG CTCTGGCACA AGATTTTGCT GTCAATCTGG GACCAAGATT GCAGTGGTAT 
TTGAAGCTAA AATCCTGGTG GGCTACCAAT TATGTGAGCG ACTGGTGGGA GGAATACATC 
TACCTCCGAG GACGAGGGCC GCTGATGGTG AACAGCAACT ACTTCGCGAT GGACTTGCTG 
TATATCATAC CGACTCACAT CCAGGCGGCA AGAGCTGGCA ACGGGATCCA CGCCATCCTG 
CTTTACAGAC GCAAACTGGA CCGGGAGGAA ATCAAACCGA TTCTTCTTCT GGGGTCTACG 
ATTCCGCTCT GCTCCGCCCA GTGGGAGCGC ATGTTTAATA CTTCTCGGAT CCCGGGAGAG 
GAGACAGACA CCATCCAGCA CCTGAGGGAC AGCAAGCACA TCGTCGTCTT CCACCGGGGC 
CGCTACTTCA AGGTCTGGCT CTACCACGAC GGCCGGCTGC TGAGGCCCCG CGAGATCGAG 
CAGCAGGTGC AGCGGATCCT GGAGGACCCG TCGGAGCCGC AGCCCGGGGA GGCCAAGCTG 
GCGGCGCTTA CGGCGGCGGA CAGGGTCCCC TGGGCCAGGT GTCGCCAGGC CTATTTTGGA 
CACGGGAAAA ACAAGCAGTC TCTGGATGCC GTGGAAAAAG CAGCGTTCTT CGTGACGTTA 
GACGAAACCG AGCAGGGATA CAGGGAGGAG GACCCGGAAA CGTCGATGGA CAGCTACGCC 
AAGTCCCTGC TGCATGGCAG GTGTTTCGAC AGGTGGTTTG ATAAATCATT CACGTTTATC 
GTCTTTAAAA ACGGAAAGAT GGGCATTAAT GCGGAGCACT CCTGGGCCGA TGCGCCCATC 
ATGGGTCACC TTTGGGAGTA CGTCATGGCC ACAGACAGCT TCCAGCTGGG TTACACGGAG 
GATGGACACT GTAAAGGAGA CACCAACCCG AACATCCCGT ACCCTACCCG GCTGCAGTGG 
GACATCCCGG AGGAATGTCA GGAGGTCATT GAGACTTCGC TGAGCAGTGC CAACCTCCTG 
GCCAACGACG TGGATTTCCA CTCCTTCCCA TTCCGCACTT TCGGTAAAGG GCTGATTAAG 
AAATGTAAAA CCAGCCCGGA CGCCTTCGTA CAGCTCTCGC TCCAGCTGGC TCATTACAAG 
GACATGGGTA AATTTTCCCT CACCTACGAG GCCTCCATGA CCCGGCTGTT CCGAGAGGGC 
AGAACGGAGA CCGTGCGTTC CTGCACCGCT GAGTCGTGCA GCTTCGTGCT GGCCATGGTA 
GACCCCACAC AGACGGTTGA ACAGAAGCTC AAGTTGTTCA GGATCGCGTC TGAGAAGCAT 
CAGCTTCTGT ACCGCCTGGC GATGACCGGT GCGGGGATCG ACCGCCACCT CTTCTGCCTC 
TACGTGGTGT CCAAGTACCT CGCGGTCGAC TCCCCTTTCC TGAAGGAGGT CTTAGCTGAG 
CCTTGGAGAT TATCCACCAG CCAGACCCCT CCACAGCAGG TGGAACTGTT CAATTTGGAT 
AGGAATCCAG ACTACGTGTC GTGCGGAGGG GGCTTCGGGC CGGTTGCTGA TGACGGCTAT 
GGGGTGTCGT ACATTCTCGT CGGTGAGGAC CTCATCAATT TCCACATATC CTCCAAGTTC 
TCCTGTCCTG AGACGGATTC GCATCGCTTC GGGAAGAACC TGAGAGAAGC AATGACCGAC 
ATCATCAGTT TGTTCGGGTT CAGCCCCAAT TCCAAAAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CPT1A ( XM_024987034.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024842802.1 Bos taurus carnitine palmitoyltransferase 1A (CPT1A), transcript variant X2, mRNA.

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CloneID	OBa231885
Clone ID Related Accession (Same CDS sequence)	XM_024987034.1
Accession Version	XM_024987034.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2178bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	carnitine O-palmitoyltransferase 1, liver isoform isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037356.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGG CCCACCAAGC CGTGGCCTTC CAGTTCACAG TCACTCCAGA TGGGATCGAC 
CTCCGGATGA GCCATGAGGC CCTCAGGCAG ATCTATCTGT CTGGTCTTCA TTCCTGGAAG 
AAGAAGTTCA TCAGATTCAA GAATGGCATC ATCACCGGCG TGTACCCAGC GAGTCCCTCC 
AGTTGGCTCA TCGTGGTTGT GGGCGTGATG TCGACCATGT ACGCCAAGAT CGACCCCTCT 
TTGGGAATAA TCGCTAAGAT CAATCGGACC CTGGACACGA CTGGCTGCAT GTCCAGCCAG 
ACCAAGAAGG TGGTGAGCGG CGTCATCTTC GGCACGGGGC TGTGGGTGGC CCTCATCGTC 
ACCATGCGTT ACTCCCTGAA GGTGCTGCTG TCCTACCACG GCTGGATGTT CACCCAGCAC 
GGCAGGTTGA GCCGCACCAC CAAGATCTGG ATGTTTATGG TCAAGATCTT CTCAGGCCGA 
AAACCCATGT TGTACAGCTT CCAGACGTCT CTGCCGCGCC TGCCCGTGCC AGCTGTCCAG 
GACACCGTCA ACAGGTACTT GGAATCTGTG AAGCCTCTTA TGAAGGAGGG AGACTTTACA 
CGGATGACGG CTCTGGCACA AGATTTTGCT GTCAATCTGG GACCAAGATT GCAGTGGTAT 
TTGAAGCTAA AATCCTGGTG GGCTACCAAT TATGTGAGCG ACTGGTGGGA GGAATACATC 
TACCTCCGAG GACGAGGGCC GCTGATGGTG AACAGCAACT ACTTCGCGAT GGACTTGCTG 
TATATCATAC CGACTCACAT CCAGGCGGCA AGAGCTGGCA ACGGGATCCA CGCCATCCTG 
CTTTACAGAC GCAAACTGGA CCGGGAGGAA ATCAAACCGA TTCTTCTTCT GGGGTCTACG 
ATTCCGCTCT GCTCCGCCCA GTGGGAGCGC ATGTTTAATA CTTCTCGGAT CCCGGGAGAG 
GAGACAGACA CCATCCAGCA CCTGAGGGAC AGCAAGCACA TCGTCGTCTT CCACCGGGGC 
CGCTACTTCA AGGTCTGGCT CTACCACGAC GGCCGGCTGC TGAGGCCCCG CGAGATCGAG 
CAGCAGGTGC AGCGGATCCT GGAGGACCCG TCGGAGCCGC AGCCCGGGGA GGCCAAGCTG 
GCGGCGCTTA CGGCGGCGGA CAGGGTCCCC TGGGCCAGGT GTCGCCAGGC CTATTTTGGA 
CACGGGAAAA ACAAGCAGTC TCTGGATGCC GTGGAAAAAG CAGCGTTCTT CGTGACGTTA 
GACGAAACCG AGCAGGGATA CAGGGAGGAG GACCCGGAAA CGTCGATGGA CAGCTACGCC 
AAGTCCCTGC TGCATGGCAG GTGTTTCGAC AGGTGGTTTG ATAAATCATT CACGTTTATC 
GTCTTTAAAA ACGGAAAGAT GGGCATTAAT GCGGAGCACT CCTGGGCCGA TGCGCCCATC 
ATGGGTCACC TTTGGGAGTA CGTCATGGCC ACAGACAGCT TCCAGCTGGG TTACACGGAG 
GATGGACACT GTAAAGGAGA CACCAACCCG AACATCCCGT ACCCTACCCG GCTGCAGTGG 
GACATCCCGG AGGAATGTCA GGAGGTCATT GAGACTTCGC TGAGCAGTGC CAACCTCCTG 
GCCAACGACG TGGATTTCCA CTCCTTCCCA TTCCGCACTT TCGGTAAAGG GCTGATTAAG 
AAATGTAAAA CCAGCCCGGA CGCCTTCGTA CAGCTCTCGC TCCAGCTGGC TCATTACAAG 
GACATGGGTA AATTTTCCCT CACCTACGAG GCCTCCATGA CCCGGCTGTT CCGAGAGGGC 
AGAACGGAGA CCGTGCGTTC CTGCACCGCT GAGTCGTGCA GCTTCGTGCT GGCCATGGTA 
GACCCCACAC AGACGGTTGA ACAGAAGCTC AAGTTGTTCA GGATCGCGTC TGAGAAGCAT 
CAGCTTCTGT ACCGCCTGGC GATGACCGGT GCGGGGATCG ACCGCCACCT CTTCTGCCTC 
TACGTGGTGT CCAAGTACCT CGCGGTCGAC TCCCCTTTCC TGAAGGAGGC ACCTTGGAAA 
ATTCCTGGAA GTTTGACCCT CAGCTTAGGA GAGCTGATAC CAGTCTGCTC AGCACACAGC 
TGCTCCCTTC CAAATCTTAC TCCGTGTATC CGCCACCTTT TCTGCCCAAG ACCCGTCAGC 
CAGTGTTTGC CTCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024842802.1
CDS	170..2347
Translation	MAEAHQAVAFQFTVTPDGIDLRMSHEALRQIYLSGLHSWKKKFIRFKNGIITGVYPASPSSWLIVVVGVMSTMYAKIDPSLGIIAKINRTLDTTGCMSSQTKKVVSGVIFGTGLWVALIVTMRYSLKVLLSYHGWMFTQHGRLSRTTKIWMFMVKIFSGRKPMLYSFQTSLPRLPVPAVQDTVNRYLESVKPLMKEGDFTRMTALAQDFAVNLGPRLQWYLKLKSWWATNYVSDWWEEYIYLRGRGPLMVNSNYFAMDLLYIIPTHIQAARAGNGIHAILLYRRKLDREEIKPILLLGSTIPLCSAQWERMFNTSRIPGEETDTIQHLRDSKHIVVFHRGRYFKVWLYHDGRLLRPREIEQQVQRILEDPSEPQPGEAKLAALTAADRVPWARCRQAYFGHGKNKQSLDAVEKAAFFVTLDETEQGYREEDPETSMDSYAKSLLHGRCFDRWFDKSFTFIVFKNGKMGINAEHSWADAPIMGHLWEYVMATDSFQLGYTEDGHCKGDTNPNIPYPTRLQWDIPEECQEVIETSLSSANLLANDVDFHSFPFRTFGKGLIKKCKTSPDAFVQLSLQLAHYKDMGKFSLTYEASMTRLFREGRTETVRSCTAESCSFVLAMVDPTQTVEQKLKLFRIASEKHQLLYRLAMTGAGIDRHLFCLYVVSKYLAVDSPFLKEAPWKIPGSLTLSLGELIPVCSAHSCSLPNLTPCIRHLFCPRPVSQCLPL

Target ORF information:

RefSeq Version	XM_024987034.1
Organism	Bos taurus(cattle)
Definition	Bos taurus carnitine palmitoyltransferase 1A (CPT1A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024987034.1

ORF Insert Sequence:

ATGGCGGAGG CCCACCAAGC CGTGGCCTTC CAGTTCACAG TCACTCCAGA TGGGATCGAC 
CTCCGGATGA GCCATGAGGC CCTCAGGCAG ATCTATCTGT CTGGTCTTCA TTCCTGGAAG 
AAGAAGTTCA TCAGATTCAA GAATGGCATC ATCACCGGCG TGTACCCAGC GAGTCCCTCC 
AGTTGGCTCA TCGTGGTTGT GGGCGTGATG TCGACCATGT ACGCCAAGAT CGACCCCTCT 
TTGGGAATAA TCGCTAAGAT CAATCGGACC CTGGACACGA CTGGCTGCAT GTCCAGCCAG 
ACCAAGAAGG TGGTGAGCGG CGTCATCTTC GGCACGGGGC TGTGGGTGGC CCTCATCGTC 
ACCATGCGTT ACTCCCTGAA GGTGCTGCTG TCCTACCACG GCTGGATGTT CACCCAGCAC 
GGCAGGTTGA GCCGCACCAC CAAGATCTGG ATGTTTATGG TCAAGATCTT CTCAGGCCGA 
AAACCCATGT TGTACAGCTT CCAGACGTCT CTGCCGCGCC TGCCCGTGCC AGCTGTCCAG 
GACACCGTCA ACAGGTACTT GGAATCTGTG AAGCCTCTTA TGAAGGAGGG AGACTTTACA 
CGGATGACGG CTCTGGCACA AGATTTTGCT GTCAATCTGG GACCAAGATT GCAGTGGTAT 
TTGAAGCTAA AATCCTGGTG GGCTACCAAT TATGTGAGCG ACTGGTGGGA GGAATACATC 
TACCTCCGAG GACGAGGGCC GCTGATGGTG AACAGCAACT ACTTCGCGAT GGACTTGCTG 
TATATCATAC CGACTCACAT CCAGGCGGCA AGAGCTGGCA ACGGGATCCA CGCCATCCTG 
CTTTACAGAC GCAAACTGGA CCGGGAGGAA ATCAAACCGA TTCTTCTTCT GGGGTCTACG 
ATTCCGCTCT GCTCCGCCCA GTGGGAGCGC ATGTTTAATA CTTCTCGGAT CCCGGGAGAG 
GAGACAGACA CCATCCAGCA CCTGAGGGAC AGCAAGCACA TCGTCGTCTT CCACCGGGGC 
CGCTACTTCA AGGTCTGGCT CTACCACGAC GGCCGGCTGC TGAGGCCCCG CGAGATCGAG 
CAGCAGGTGC AGCGGATCCT GGAGGACCCG TCGGAGCCGC AGCCCGGGGA GGCCAAGCTG 
GCGGCGCTTA CGGCGGCGGA CAGGGTCCCC TGGGCCAGGT GTCGCCAGGC CTATTTTGGA 
CACGGGAAAA ACAAGCAGTC TCTGGATGCC GTGGAAAAAG CAGCGTTCTT CGTGACGTTA 
GACGAAACCG AGCAGGGATA CAGGGAGGAG GACCCGGAAA CGTCGATGGA CAGCTACGCC 
AAGTCCCTGC TGCATGGCAG GTGTTTCGAC AGGTGGTTTG ATAAATCATT CACGTTTATC 
GTCTTTAAAA ACGGAAAGAT GGGCATTAAT GCGGAGCACT CCTGGGCCGA TGCGCCCATC 
ATGGGTCACC TTTGGGAGTA CGTCATGGCC ACAGACAGCT TCCAGCTGGG TTACACGGAG 
GATGGACACT GTAAAGGAGA CACCAACCCG AACATCCCGT ACCCTACCCG GCTGCAGTGG 
GACATCCCGG AGGAATGTCA GGAGGTCATT GAGACTTCGC TGAGCAGTGC CAACCTCCTG 
GCCAACGACG TGGATTTCCA CTCCTTCCCA TTCCGCACTT TCGGTAAAGG GCTGATTAAG 
AAATGTAAAA CCAGCCCGGA CGCCTTCGTA CAGCTCTCGC TCCAGCTGGC TCATTACAAG 
GACATGGGTA AATTTTCCCT CACCTACGAG GCCTCCATGA CCCGGCTGTT CCGAGAGGGC 
AGAACGGAGA CCGTGCGTTC CTGCACCGCT GAGTCGTGCA GCTTCGTGCT GGCCATGGTA 
GACCCCACAC AGACGGTTGA ACAGAAGCTC AAGTTGTTCA GGATCGCGTC TGAGAAGCAT 
CAGCTTCTGT ACCGCCTGGC GATGACCGGT GCGGGGATCG ACCGCCACCT CTTCTGCCTC 
TACGTGGTGT CCAAGTACCT CGCGGTCGAC TCCCCTTTCC TGAAGGAGGC ACCTTGGAAA 
ATTCCTGGAA GTTTGACCCT CAGCTTAGGA GAGCTGATAC CAGTCTGCTC AGCACACAGC 
TGCTCCCTTC CAAATCTTAC TCCGTGTATC CGCCACCTTT TCTGCCCAAG ACCCGTCAGC 
CAGTGTTTGC CTCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Effect of NEFA and glucose levels on CPT-I mRNA expression and translation in cultured bovine hepatocytes.
The Journal of veterinary medical science73(1)97-101(2011 Jan)
Xu C,Wang Z,Zhang RH,Zhang HY,Fu SX,Xia C

Activity of hepatic but not skeletal muscle carnitine palmitoyltransferase enzyme is depressed by intravenous glucose infusions in lactating dairy cows.
Journal of animal physiology and animal nutrition94(6)685-95(2010 Dec)
Al-Trad B,Wittek T,G?bel G,F?rll M,Reisberg K,Aschenbach JR

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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CPT1A cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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