Cpt1a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cpt1a
Entrez Gene ID	25757
Full Name	carnitine palmitoyltransferase 1A
Synonyms	CPT-Ia
General protein information	Preferred Names carnitine palmitoyltransferase 1A Names carnitine O-palmitoyltransferase 1, liver isoform CPT I CPT1-L CPTI-L Carnitine palmitoyltransferase 1 alpha, liver isoform carnitine O-palmitoyltransferase I, liver isoform carnitine palmitoyltransferase 1, liver carnitine palmitoyltransferase 1a, liver
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q42
Summary	enzyme that catalyses the transfer of long chain fatty acids to carnitine for translocation across the mitochondrial inner membrane [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017588838.1	XP_017444327.1	carnitine O-palmitoyltransferase 1, liver isoform isoform X2
NM_031559.2	NP_113747.2	carnitine O-palmitoyltransferase 1, liver isoform
XM_006230695.2	XP_006230757.1	carnitine O-palmitoyltransferase 1, liver isoform isoform X1
XM_017588837.1	XP_017444326.1	carnitine O-palmitoyltransferase 1, liver isoform isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Cpt1a	NP_038523.2
Bos taurus (cattle)	CPT1A	XP_005227433.1
Gallus gallus (chicken)	CPT1A	NP_001012916.1
Danio rerio (zebrafish)	si:ch211-236l14.3	XP_005170764.1
	CPT1A	XP_001101846.1
Rattus norvegicus (Norway rat)	Cpt1a	NP_113747.2
Danio rerio (zebrafish)	cpt1a	NP_001038319.1
Pan troglodytes (chimpanzee)	CPT1A	XP_001173853.1
Canis lupus familiaris (dog)	CPT1A	XP_533208.3
Homo sapiens (human)	CPT1A	NP_001867.2
Xenopus tropicalis (tropical clawed frog)	LOC100490391	XP_004913491.1

Gene Ontology
Bibliography

Related articles in PubMed

Obesity-alleviating potential of asiatic acid and its effects on ACC1, UCP2, and CPT1 mRNA expression in high fat diet-induced obese Sprague-Dawley rats.
Rameshreddy P, Uddandrao VVS, Brahmanaidu P, Vadivukkarasi S, Ravindarnaik R, Suresh P, Swapna K, Kalaivani A, Parvathi P, Tamilmani P, Saravanan G
Molecular and cellular biochemistry(2017 Oct)

Examination of carnitine palmitoyltransferase 1 abundance in white adipose tissue: implications in obesity research.
Warfel JD, Vandanmagsar B, Dubuisson OS, Hodgeson SM, Elks CM, Ravussin E, Mynatt RL
American journal of physiology. Regulatory, integrative and comparative physiology312(5)R816-R820(2017 May)

Type of supplemented simple sugar, not merely calorie intake, determines adverse effects on metabolism and aortic function in female rats.
Sang?esa G, Shaligram S, Akther F, Roglans N, Laguna JC, Rahimian R, Alegret M
American journal of physiology. Heart and circulatory physiology312(2)H289-H304(2017 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Furthermore, given the already low abundance of Cpt1b in white adipose tissue, it is unlikely that decreases in its expression can quantitatively decrease whole body energy expenditure enough to contribute to an obese phenotype.
Title: Examination of carnitine palmitoyltransferase 1 abundance in white adipose tissue: implications in obesity research.

e found that CPT1AM-expressing rBA have increased FAO, lipolysis, UCP1 protein levels and mitochondrial activity. Additionally, enhanced FAO reduced the palmitate-induced increase in triglyceride content and the expression of obese and inflammatory markers. Thus, CPT1AM-expressing rBA had enhanced fat-burning capacity and improved lipid-induced derangements.
Title: Carnitine Palmitoyltransferase 1 Increases Lipolysis, UCP1 Protein Expression and Mitochondrial Activity in Brown Adipocytes.

Fructose feeding significantly decreased mRNA levels for PPAR alpha and CPT1A.
Title: High fructose consumption induces DNA methylation at PPAR? and CPT1A promoter regions in the rat liver.

Tau supplementation prevented obesity and hepatic TG deposition by upregulating MTP mRNA, ameliorating hepatic lipid efflux, and consequently enhancing PPAR-alpha which increases lipid oxidation through ACO and CPT-1a gene expressions.
Title: Improvement in the expression of hepatic genes involved in fatty acid metabolism in obese rats supplemented with taurine.

upregulation CPT1 expression leading to reduction in the accumulation of lipid intermediates in skeletal muscle.
Title: Mitofusion-2-mediated alleviation of insulin resistance in rats through reduction in lipid intermediate accumulation in skeletal muscle.

The following Cpt1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cpt1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa12112	NM_031559.2 Latest version!	Rattus norvegicus carnitine palmitoyltransferase 1A (Cpt1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
ORa12112	XM_017588837.1 Latest version!	Rattus norvegicus carnitine palmitoyltransferase 1A (Cpt1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
ORa12112	XM_006230695.2 Latest version!	Rattus norvegicus carnitine palmitoyltransferase 1A (Cpt1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
ORa56414	XM_017588838.1 Latest version!	Rattus norvegicus carnitine palmitoyltransferase 1A (Cpt1a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cpt1a ( NM_031559.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_113747.2 Rattus norvegicus carnitine palmitoyltransferase 1A (Cpt1a), mRNA.

Price & Availability↑

CloneID	ORa12112
Clone ID Related Accession (Same CDS sequence)	NM_031559.2 , XM_006230695.2 , XM_017588837.1
Accession Version	NM_031559.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-16
Organism	Rattus norvegicus(Norway rat)
Product	carnitine O-palmitoyltransferase 1, liver isoform
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC072522.1 and AA875270.1. On Dec 11, 2007 this sequence version replaced NM_031559.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC072522.1, L07736.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGCAGAGG CTCACCAAGC TGTGGCCTTC CAGTTCACTG TCACCCCCGA TGGCATTGAC 
CTCCGCCTGA GCCACGAAGC CCTCAAACAG ATCTGCCTGT CGGGGCTGCA CTCCTGGAAG 
AAGAAGTTCA TCCGGTTCAA GAATGGCATC ATCACTGGTG TGTTCCCCGC GAATCCGTCC 
AGCTGGCTTA TCGTGGTGGT GGGTGTGATT TCATCCATGC ATGCCAAAGT GGACCCCTCC 
CTGGGCATGA TCGCAAAGAT CAGTCGGACC CTAGACACCA CTGGCCGAAT GTCAAGCCAG 
ACGAAGAACA TTGTGAGCGG CGTCCTCTTT GGTACAGGGC TCTGGGTGGC AGTCATCATG 
ACCATGCGCT ACTCGCTGAA GGTGCTGCTC TCCTACCACG GCTGGATGTT TGCAGAACAC 
GGCAAAATGA GCCGCAGCAC CAAGATCTGG ATGGCTATGG TCAAGGTCCT CTCAGGTCGG 
AAGCCCATGT TGTACAGCTT CCAGACGTCT CTGCCACGCC TGCCTGTCCC AGCTGTCAAA 
GATACTGTGA GCAGGTACCT GGAATCTGTG AGGCCACTGA TGAAGGAAGA AGACTTCCAG 
CGCATGACAG CACTGGCCCA GGATTTTGCT GTCAACCTCG GACCCAAATT GCAGTGGTAT 
TTGAAGCTAA AATCCTGGTG GGCCACAAAT TACGTGAGTG ACTGGTGGGA AGAATATATC 
TACCTGCGGG GCCGAGGGCC GCTCATGGTC AACAGCAACT ACTACGCCAT GGAGATGCTG 
TACATCACCC CAACCCATAT CCAGGCAGCG AGAGCTGGCA ACACCATCCA CGCCATACTG 
CTGTATCGTC GCACATTAGA CCGTGAGGAA CTCAAACCCA TTCGTCTTCT GGGATCCACC 
ATTCCACTCT GCTCGGCCCA GTGGGAGCGA CTCTTCAATA CTTCCCGGAT CCCTGGGGAA 
GAGACAGACA CCATCCAACA TATCAAGGAC AGCAGGCACA TTGTTGTGTA CCACAGAGGG 
CGGTACTTCA AGGTCTGGCT CTACCACGAT GGGAGGCTGC TGAGGCCCCG AGAGCTGGAG 
CAGCAGATGC AGCAGATCCT GGATGATCCC TCAGAGCCAC AGCCTGGGGA GGCCAAGCTG 
GCCGCCCTCA CTGCTGCAGA CAGAGTGCCC TGGGCAAAGT GTCGGCAGAC CTATTTTGCA 
CGAGGGAAAA ATAAGCAGTC CCTGGATGCG GTGGAAAAGG CAGCGTTCTT CGTGACGTTG 
GACGAATCGG AGCAGGGATA CAGAGAGGAG GATCCTGAGG CATCCATCGA CAGCTACGCC 
AAATCCCTGC TGCATGGAAG ATGCTTTGAC AGGTGGTTTG ACAAGTCCAT CACCTTTGTT 
GTCTTCAAAA ACAGCAAGAT AGGCATAAAT GCAGAGCACT CCTGGGCGGA CGCGCCCGTC 
GTGGGCCATT TGTGGGAGTA TGTCATGGCC ACCGACGTCT TCCAGCTGGG TTACTCAGAG 
GATGGACACT GTAAAGGAGA CACCAACCCC AACATCCCTA AGCCCACAAG GCTACAATGG 
GACATTCCAG GAGAGTGCCA GGAGGTCATA GATGCATCCC TGAGCAGCGC CAGTCTTTTG 
GCAAATGATG TGGACCTGCA TTCCTTCCCA TTTGACTCTT TCGGCAAAGG CTTGATCAAG 
AAGTGCCGGA CGAGTCCCGA TGCCTTCATC CAGCTGGCGC TGCAGCTCGC ACATTACAAG 
GACATGGGCA AGTTCTGCCT CACATATGAG GCCTCCATGA CCCGGCTCTT CCGAGAAGGG 
AGGACAGAGA CTGTACGCTC CTGCACTATG GAGTCCTGCA ACTTTGTGCA GGCCATGATG 
GACCCCAAGT CAACGGCAGA GCAGAGGCTC AAGCTGTTCA AGATAGCTTG TGAGAAGCAC 
CAGCACCTGT ACCGCCTCGC CATGACGGGC GCCGGCATCG ACCGCCATCT CTTCTGCCTC 
TATGTGGTGT CCAAGTATCT TGCAGTCGAC TCACCTTTCC TGAAGGAGGT ATTGTCTGAG 
CCATGGAGGT TGTCTACGAG CCAGACTCCT CAGCAGCAGG TGGAGCTCTT TGACTTTGAG 
AAAAACCCTG ACTATGTGTC CTGTGGAGGG GGCTTTGGGC CGGTTGCCGA TGACGGCTAT 
GGTGTCTCCT ACATTATAGT GGGAGAGAAT TTTATCCACT TCCATATTTC TTCCAAGTTC 
TCTAGCCCTG AGACAGACTC ACACCGCTTT GGGAAGCACT TGAGACAAGC CATGATGGAC 
ATTATCACCT TGTTTGGCCT CACCATCAAT TCTAAAAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113747.2
CDS	49..2370
Translation	MAEAHQAVAFQFTVTPDGIDLRLSHEALKQICLSGLHSWKKKFIRFKNGIITGVFPANPSSWLIVVVGVISSMHAKVDPSLGMIAKISRTLDTTGRMSSQTKNIVSGVLFGTGLWVAVIMTMRYSLKVLLSYHGWMFAEHGKMSRSTKIWMAMVKVLSGRKPMLYSFQTSLPRLPVPAVKDTVSRYLESVRPLMKEEDFQRMTALAQDFAVNLGPKLQWYLKLKSWWATNYVSDWWEEYIYLRGRGPLMVNSNYYAMEMLYITPTHIQAARAGNTIHAILLYRRTLDREELKPIRLLGSTIPLCSAQWERLFNTSRIPGEETDTIQHIKDSRHIVVYHRGRYFKVWLYHDGRLLRPRELEQQMQQILDDPSEPQPGEAKLAALTAADRVPWAKCRQTYFARGKNKQSLDAVEKAAFFVTLDESEQGYREEDPEASIDSYAKSLLHGRCFDRWFDKSITFVVFKNSKIGINAEHSWADAPVVGHLWEYVMATDVFQLGYSEDGHCKGDTNPNIPKPTRLQWDIPGECQEVIDASLSSASLLANDVDLHSFPFDSFGKGLIKKCRTSPDAFIQLALQLAHYKDMGKFCLTYEASMTRLFREGRTETVRSCTMESCNFVQAMMDPKSTAEQRLKLFKIACEKHQHLYRLAMTGAGIDRHLFCLYVVSKYLAVDSPFLKEVLSEPWRLSTSQTPQQQVELFDFEKNPDYVSCGGGFGPVADDGYGVSYIIVGENFIHFHISSKFSSPETDSHRFGKHLRQAMMDIITLFGLTINSKK

Target ORF information:

RefSeq Version	NM_031559.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus carnitine palmitoyltransferase 1A (Cpt1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031559.2

ORF Insert Sequence:

ATGGCAGAGG CTCACCAAGC TGTGGCCTTC CAGTTCACTG TCACCCCCGA TGGCATTGAC 
CTCCGCCTGA GCCACGAAGC CCTCAAACAG ATCTGCCTGT CGGGGCTGCA CTCCTGGAAG 
AAGAAGTTCA TCCGGTTCAA GAATGGCATC ATCACTGGTG TGTTCCCCGC GAATCCGTCC 
AGCTGGCTTA TCGTGGTGGT GGGTGTGATT TCATCCATGC ATGCCAAAGT GGACCCCTCC 
CTGGGCATGA TCGCAAAGAT CAGTCGGACC CTAGACACCA CTGGCCGAAT GTCAAGCCAG 
ACGAAGAACA TTGTGAGCGG CGTCCTCTTT GGTACAGGGC TCTGGGTGGC AGTCATCATG 
ACCATGCGCT ACTCGCTGAA GGTGCTGCTC TCCTACCACG GCTGGATGTT TGCAGAACAC 
GGCAAAATGA GCCGCAGCAC CAAGATCTGG ATGGCTATGG TCAAGGTCCT CTCAGGTCGG 
AAGCCCATGT TGTACAGCTT CCAGACGTCT CTGCCACGCC TGCCTGTCCC AGCTGTCAAA 
GATACTGTGA GCAGGTACCT GGAATCTGTG AGGCCACTGA TGAAGGAAGA AGACTTCCAG 
CGCATGACAG CACTGGCCCA GGATTTTGCT GTCAACCTCG GACCCAAATT GCAGTGGTAT 
TTGAAGCTAA AATCCTGGTG GGCCACAAAT TACGTGAGTG ACTGGTGGGA AGAATATATC 
TACCTGCGGG GCCGAGGGCC GCTCATGGTC AACAGCAACT ACTACGCCAT GGAGATGCTG 
TACATCACCC CAACCCATAT CCAGGCAGCG AGAGCTGGCA ACACCATCCA CGCCATACTG 
CTGTATCGTC GCACATTAGA CCGTGAGGAA CTCAAACCCA TTCGTCTTCT GGGATCCACC 
ATTCCACTCT GCTCGGCCCA GTGGGAGCGA CTCTTCAATA CTTCCCGGAT CCCTGGGGAA 
GAGACAGACA CCATCCAACA TATCAAGGAC AGCAGGCACA TTGTTGTGTA CCACAGAGGG 
CGGTACTTCA AGGTCTGGCT CTACCACGAT GGGAGGCTGC TGAGGCCCCG AGAGCTGGAG 
CAGCAGATGC AGCAGATCCT GGATGATCCC TCAGAGCCAC AGCCTGGGGA GGCCAAGCTG 
GCCGCCCTCA CTGCTGCAGA CAGAGTGCCC TGGGCAAAGT GTCGGCAGAC CTATTTTGCA 
CGAGGGAAAA ATAAGCAGTC CCTGGATGCG GTGGAAAAGG CAGCGTTCTT CGTGACGTTG 
GACGAATCGG AGCAGGGATA CAGAGAGGAG GATCCTGAGG CATCCATCGA CAGCTACGCC 
AAATCCCTGC TGCATGGAAG ATGCTTTGAC AGGTGGTTTG ACAAGTCCAT CACCTTTGTT 
GTCTTCAAAA ACAGCAAGAT AGGCATAAAT GCAGAGCACT CCTGGGCGGA CGCGCCCGTC 
GTGGGCCATT TGTGGGAGTA TGTCATGGCC ACCGACGTCT TCCAGCTGGG TTACTCAGAG 
GATGGACACT GTAAAGGAGA CACCAACCCC AACATCCCTA AGCCCACAAG GCTACAATGG 
GACATTCCAG GAGAGTGCCA GGAGGTCATA GATGCATCCC TGAGCAGCGC CAGTCTTTTG 
GCAAATGATG TGGACCTGCA TTCCTTCCCA TTTGACTCTT TCGGCAAAGG CTTGATCAAG 
AAGTGCCGGA CGAGTCCCGA TGCCTTCATC CAGCTGGCGC TGCAGCTCGC ACATTACAAG 
GACATGGGCA AGTTCTGCCT CACATATGAG GCCTCCATGA CCCGGCTCTT CCGAGAAGGG 
AGGACAGAGA CTGTACGCTC CTGCACTATG GAGTCCTGCA ACTTTGTGCA GGCCATGATG 
GACCCCAAGT CAACGGCAGA GCAGAGGCTC AAGCTGTTCA AGATAGCTTG TGAGAAGCAC 
CAGCACCTGT ACCGCCTCGC CATGACGGGC GCCGGCATCG ACCGCCATCT CTTCTGCCTC 
TATGTGGTGT CCAAGTATCT TGCAGTCGAC TCACCTTTCC TGAAGGAGGT ATTGTCTGAG 
CCATGGAGGT TGTCTACGAG CCAGACTCCT CAGCAGCAGG TGGAGCTCTT TGACTTTGAG 
AAAAACCCTG ACTATGTGTC CTGTGGAGGG GGCTTTGGGC CGGTTGCCGA TGACGGCTAT 
GGTGTCTCCT ACATTATAGT GGGAGAGAAT TTTATCCACT TCCATATTTC TTCCAAGTTC 
TCTAGCCCTG AGACAGACTC ACACCGCTTT GGGAAGCACT TGAGACAAGC CATGATGGAC 
ATTATCACCT TGTTTGGCCT CACCATCAAT TCTAAAAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cpt1a ( XM_006230695.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006230757.1 Rattus norvegicus carnitine palmitoyltransferase 1A (Cpt1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa12112
Clone ID Related Accession (Same CDS sequence)	NM_031559.2 , XM_006230695.2 , XM_017588837.1
Accession Version	XM_006230695.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	carnitine O-palmitoyltransferase 1, liver isoform isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006230695.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGG CTCACCAAGC TGTGGCCTTC CAGTTCACTG TCACCCCCGA TGGCATTGAC 
CTCCGCCTGA GCCACGAAGC CCTCAAACAG ATCTGCCTGT CGGGGCTGCA CTCCTGGAAG 
AAGAAGTTCA TCCGGTTCAA GAATGGCATC ATCACTGGTG TGTTCCCCGC GAATCCGTCC 
AGCTGGCTTA TCGTGGTGGT GGGTGTGATT TCATCCATGC ATGCCAAAGT GGACCCCTCC 
CTGGGCATGA TCGCAAAGAT CAGTCGGACC CTAGACACCA CTGGCCGAAT GTCAAGCCAG 
ACGAAGAACA TTGTGAGCGG CGTCCTCTTT GGTACAGGGC TCTGGGTGGC AGTCATCATG 
ACCATGCGCT ACTCGCTGAA GGTGCTGCTC TCCTACCACG GCTGGATGTT TGCAGAACAC 
GGCAAAATGA GCCGCAGCAC CAAGATCTGG ATGGCTATGG TCAAGGTCCT CTCAGGTCGG 
AAGCCCATGT TGTACAGCTT CCAGACGTCT CTGCCACGCC TGCCTGTCCC AGCTGTCAAA 
GATACTGTGA GCAGGTACCT GGAATCTGTG AGGCCACTGA TGAAGGAAGA AGACTTCCAG 
CGCATGACAG CACTGGCCCA GGATTTTGCT GTCAACCTCG GACCCAAATT GCAGTGGTAT 
TTGAAGCTAA AATCCTGGTG GGCCACAAAT TACGTGAGTG ACTGGTGGGA AGAATATATC 
TACCTGCGGG GCCGAGGGCC GCTCATGGTC AACAGCAACT ACTACGCCAT GGAGATGCTG 
TACATCACCC CAACCCATAT CCAGGCAGCG AGAGCTGGCA ACACCATCCA CGCCATACTG 
CTGTATCGTC GCACATTAGA CCGTGAGGAA CTCAAACCCA TTCGTCTTCT GGGATCCACC 
ATTCCACTCT GCTCGGCCCA GTGGGAGCGA CTCTTCAATA CTTCCCGGAT CCCTGGGGAA 
GAGACAGACA CCATCCAACA TATCAAGGAC AGCAGGCACA TTGTTGTGTA CCACAGAGGG 
CGGTACTTCA AGGTCTGGCT CTACCACGAT GGGAGGCTGC TGAGGCCCCG AGAGCTGGAG 
CAGCAGATGC AGCAGATCCT GGATGATCCC TCAGAGCCAC AGCCTGGGGA GGCCAAGCTG 
GCCGCCCTCA CTGCTGCAGA CAGAGTGCCC TGGGCAAAGT GTCGGCAGAC CTATTTTGCA 
CGAGGGAAAA ATAAGCAGTC CCTGGATGCG GTGGAAAAGG CAGCGTTCTT CGTGACGTTG 
GACGAATCGG AGCAGGGATA CAGAGAGGAG GATCCTGAGG CATCCATCGA CAGCTACGCC 
AAATCCCTGC TGCATGGAAG ATGCTTTGAC AGGTGGTTTG ACAAGTCCAT CACCTTTGTT 
GTCTTCAAAA ACAGCAAGAT AGGCATAAAT GCAGAGCACT CCTGGGCGGA CGCGCCCGTC 
GTGGGCCATT TGTGGGAGTA TGTCATGGCC ACCGACGTCT TCCAGCTGGG TTACTCAGAG 
GATGGACACT GTAAAGGAGA CACCAACCCC AACATCCCTA AGCCCACAAG GCTACAATGG 
GACATTCCAG GAGAGTGCCA GGAGGTCATA GATGCATCCC TGAGCAGCGC CAGTCTTTTG 
GCAAATGATG TGGACCTGCA TTCCTTCCCA TTTGACTCTT TCGGCAAAGG CTTGATCAAG 
AAGTGCCGGA CGAGTCCCGA TGCCTTCATC CAGCTGGCGC TGCAGCTCGC ACATTACAAG 
GACATGGGCA AGTTCTGCCT CACATATGAG GCCTCCATGA CCCGGCTCTT CCGAGAAGGG 
AGGACAGAGA CTGTACGCTC CTGCACTATG GAGTCCTGCA ACTTTGTGCA GGCCATGATG 
GACCCCAAGT CAACGGCAGA GCAGAGGCTC AAGCTGTTCA AGATAGCTTG TGAGAAGCAC 
CAGCACCTGT ACCGCCTCGC CATGACGGGC GCCGGCATCG ACCGCCATCT CTTCTGCCTC 
TATGTGGTGT CCAAGTATCT TGCAGTCGAC TCACCTTTCC TGAAGGAGGT ATTGTCTGAG 
CCATGGAGGT TGTCTACGAG CCAGACTCCT CAGCAGCAGG TGGAGCTCTT TGACTTTGAG 
AAAAACCCTG ACTATGTGTC CTGTGGAGGG GGCTTTGGGC CGGTTGCCGA TGACGGCTAT 
GGTGTCTCCT ACATTATAGT GGGAGAGAAT TTTATCCACT TCCATATTTC TTCCAAGTTC 
TCTAGCCCTG AGACAGACTC ACACCGCTTT GGGAAGCACT TGAGACAAGC CATGATGGAC 
ATTATCACCT TGTTTGGCCT CACCATCAAT TCTAAAAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006230757.1
CDS	234..2555
Translation	MAEAHQAVAFQFTVTPDGIDLRLSHEALKQICLSGLHSWKKKFIRFKNGIITGVFPANPSSWLIVVVGVISSMHAKVDPSLGMIAKISRTLDTTGRMSSQTKNIVSGVLFGTGLWVAVIMTMRYSLKVLLSYHGWMFAEHGKMSRSTKIWMAMVKVLSGRKPMLYSFQTSLPRLPVPAVKDTVSRYLESVRPLMKEEDFQRMTALAQDFAVNLGPKLQWYLKLKSWWATNYVSDWWEEYIYLRGRGPLMVNSNYYAMEMLYITPTHIQAARAGNTIHAILLYRRTLDREELKPIRLLGSTIPLCSAQWERLFNTSRIPGEETDTIQHIKDSRHIVVYHRGRYFKVWLYHDGRLLRPRELEQQMQQILDDPSEPQPGEAKLAALTAADRVPWAKCRQTYFARGKNKQSLDAVEKAAFFVTLDESEQGYREEDPEASIDSYAKSLLHGRCFDRWFDKSITFVVFKNSKIGINAEHSWADAPVVGHLWEYVMATDVFQLGYSEDGHCKGDTNPNIPKPTRLQWDIPGECQEVIDASLSSASLLANDVDLHSFPFDSFGKGLIKKCRTSPDAFIQLALQLAHYKDMGKFCLTYEASMTRLFREGRTETVRSCTMESCNFVQAMMDPKSTAEQRLKLFKIACEKHQHLYRLAMTGAGIDRHLFCLYVVSKYLAVDSPFLKEVLSEPWRLSTSQTPQQQVELFDFEKNPDYVSCGGGFGPVADDGYGVSYIIVGENFIHFHISSKFSSPETDSHRFGKHLRQAMMDIITLFGLTINSKK

Target ORF information:

RefSeq Version	XM_006230695.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus carnitine palmitoyltransferase 1A (Cpt1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006230695.2

ORF Insert Sequence:

ATGGCAGAGG CTCACCAAGC TGTGGCCTTC CAGTTCACTG TCACCCCCGA TGGCATTGAC 
CTCCGCCTGA GCCACGAAGC CCTCAAACAG ATCTGCCTGT CGGGGCTGCA CTCCTGGAAG 
AAGAAGTTCA TCCGGTTCAA GAATGGCATC ATCACTGGTG TGTTCCCCGC GAATCCGTCC 
AGCTGGCTTA TCGTGGTGGT GGGTGTGATT TCATCCATGC ATGCCAAAGT GGACCCCTCC 
CTGGGCATGA TCGCAAAGAT CAGTCGGACC CTAGACACCA CTGGCCGAAT GTCAAGCCAG 
ACGAAGAACA TTGTGAGCGG CGTCCTCTTT GGTACAGGGC TCTGGGTGGC AGTCATCATG 
ACCATGCGCT ACTCGCTGAA GGTGCTGCTC TCCTACCACG GCTGGATGTT TGCAGAACAC 
GGCAAAATGA GCCGCAGCAC CAAGATCTGG ATGGCTATGG TCAAGGTCCT CTCAGGTCGG 
AAGCCCATGT TGTACAGCTT CCAGACGTCT CTGCCACGCC TGCCTGTCCC AGCTGTCAAA 
GATACTGTGA GCAGGTACCT GGAATCTGTG AGGCCACTGA TGAAGGAAGA AGACTTCCAG 
CGCATGACAG CACTGGCCCA GGATTTTGCT GTCAACCTCG GACCCAAATT GCAGTGGTAT 
TTGAAGCTAA AATCCTGGTG GGCCACAAAT TACGTGAGTG ACTGGTGGGA AGAATATATC 
TACCTGCGGG GCCGAGGGCC GCTCATGGTC AACAGCAACT ACTACGCCAT GGAGATGCTG 
TACATCACCC CAACCCATAT CCAGGCAGCG AGAGCTGGCA ACACCATCCA CGCCATACTG 
CTGTATCGTC GCACATTAGA CCGTGAGGAA CTCAAACCCA TTCGTCTTCT GGGATCCACC 
ATTCCACTCT GCTCGGCCCA GTGGGAGCGA CTCTTCAATA CTTCCCGGAT CCCTGGGGAA 
GAGACAGACA CCATCCAACA TATCAAGGAC AGCAGGCACA TTGTTGTGTA CCACAGAGGG 
CGGTACTTCA AGGTCTGGCT CTACCACGAT GGGAGGCTGC TGAGGCCCCG AGAGCTGGAG 
CAGCAGATGC AGCAGATCCT GGATGATCCC TCAGAGCCAC AGCCTGGGGA GGCCAAGCTG 
GCCGCCCTCA CTGCTGCAGA CAGAGTGCCC TGGGCAAAGT GTCGGCAGAC CTATTTTGCA 
CGAGGGAAAA ATAAGCAGTC CCTGGATGCG GTGGAAAAGG CAGCGTTCTT CGTGACGTTG 
GACGAATCGG AGCAGGGATA CAGAGAGGAG GATCCTGAGG CATCCATCGA CAGCTACGCC 
AAATCCCTGC TGCATGGAAG ATGCTTTGAC AGGTGGTTTG ACAAGTCCAT CACCTTTGTT 
GTCTTCAAAA ACAGCAAGAT AGGCATAAAT GCAGAGCACT CCTGGGCGGA CGCGCCCGTC 
GTGGGCCATT TGTGGGAGTA TGTCATGGCC ACCGACGTCT TCCAGCTGGG TTACTCAGAG 
GATGGACACT GTAAAGGAGA CACCAACCCC AACATCCCTA AGCCCACAAG GCTACAATGG 
GACATTCCAG GAGAGTGCCA GGAGGTCATA GATGCATCCC TGAGCAGCGC CAGTCTTTTG 
GCAAATGATG TGGACCTGCA TTCCTTCCCA TTTGACTCTT TCGGCAAAGG CTTGATCAAG 
AAGTGCCGGA CGAGTCCCGA TGCCTTCATC CAGCTGGCGC TGCAGCTCGC ACATTACAAG 
GACATGGGCA AGTTCTGCCT CACATATGAG GCCTCCATGA CCCGGCTCTT CCGAGAAGGG 
AGGACAGAGA CTGTACGCTC CTGCACTATG GAGTCCTGCA ACTTTGTGCA GGCCATGATG 
GACCCCAAGT CAACGGCAGA GCAGAGGCTC AAGCTGTTCA AGATAGCTTG TGAGAAGCAC 
CAGCACCTGT ACCGCCTCGC CATGACGGGC GCCGGCATCG ACCGCCATCT CTTCTGCCTC 
TATGTGGTGT CCAAGTATCT TGCAGTCGAC TCACCTTTCC TGAAGGAGGT ATTGTCTGAG 
CCATGGAGGT TGTCTACGAG CCAGACTCCT CAGCAGCAGG TGGAGCTCTT TGACTTTGAG 
AAAAACCCTG ACTATGTGTC CTGTGGAGGG GGCTTTGGGC CGGTTGCCGA TGACGGCTAT 
GGTGTCTCCT ACATTATAGT GGGAGAGAAT TTTATCCACT TCCATATTTC TTCCAAGTTC 
TCTAGCCCTG AGACAGACTC ACACCGCTTT GGGAAGCACT TGAGACAAGC CATGATGGAC 
ATTATCACCT TGTTTGGCCT CACCATCAAT TCTAAAAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cpt1a ( XM_017588837.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017444326.1 Rattus norvegicus carnitine palmitoyltransferase 1A (Cpt1a), transcript variant X2, mRNA.

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CloneID	ORa12112
Clone ID Related Accession (Same CDS sequence)	NM_031559.2 , XM_006230695.2 , XM_017588837.1
Accession Version	XM_017588837.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	carnitine O-palmitoyltransferase 1, liver isoform isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGG CTCACCAAGC TGTGGCCTTC CAGTTCACTG TCACCCCCGA TGGCATTGAC 
CTCCGCCTGA GCCACGAAGC CCTCAAACAG ATCTGCCTGT CGGGGCTGCA CTCCTGGAAG 
AAGAAGTTCA TCCGGTTCAA GAATGGCATC ATCACTGGTG TGTTCCCCGC GAATCCGTCC 
AGCTGGCTTA TCGTGGTGGT GGGTGTGATT TCATCCATGC ATGCCAAAGT GGACCCCTCC 
CTGGGCATGA TCGCAAAGAT CAGTCGGACC CTAGACACCA CTGGCCGAAT GTCAAGCCAG 
ACGAAGAACA TTGTGAGCGG CGTCCTCTTT GGTACAGGGC TCTGGGTGGC AGTCATCATG 
ACCATGCGCT ACTCGCTGAA GGTGCTGCTC TCCTACCACG GCTGGATGTT TGCAGAACAC 
GGCAAAATGA GCCGCAGCAC CAAGATCTGG ATGGCTATGG TCAAGGTCCT CTCAGGTCGG 
AAGCCCATGT TGTACAGCTT CCAGACGTCT CTGCCACGCC TGCCTGTCCC AGCTGTCAAA 
GATACTGTGA GCAGGTACCT GGAATCTGTG AGGCCACTGA TGAAGGAAGA AGACTTCCAG 
CGCATGACAG CACTGGCCCA GGATTTTGCT GTCAACCTCG GACCCAAATT GCAGTGGTAT 
TTGAAGCTAA AATCCTGGTG GGCCACAAAT TACGTGAGTG ACTGGTGGGA AGAATATATC 
TACCTGCGGG GCCGAGGGCC GCTCATGGTC AACAGCAACT ACTACGCCAT GGAGATGCTG 
TACATCACCC CAACCCATAT CCAGGCAGCG AGAGCTGGCA ACACCATCCA CGCCATACTG 
CTGTATCGTC GCACATTAGA CCGTGAGGAA CTCAAACCCA TTCGTCTTCT GGGATCCACC 
ATTCCACTCT GCTCGGCCCA GTGGGAGCGA CTCTTCAATA CTTCCCGGAT CCCTGGGGAA 
GAGACAGACA CCATCCAACA TATCAAGGAC AGCAGGCACA TTGTTGTGTA CCACAGAGGG 
CGGTACTTCA AGGTCTGGCT CTACCACGAT GGGAGGCTGC TGAGGCCCCG AGAGCTGGAG 
CAGCAGATGC AGCAGATCCT GGATGATCCC TCAGAGCCAC AGCCTGGGGA GGCCAAGCTG 
GCCGCCCTCA CTGCTGCAGA CAGAGTGCCC TGGGCAAAGT GTCGGCAGAC CTATTTTGCA 
CGAGGGAAAA ATAAGCAGTC CCTGGATGCG GTGGAAAAGG CAGCGTTCTT CGTGACGTTG 
GACGAATCGG AGCAGGGATA CAGAGAGGAG GATCCTGAGG CATCCATCGA CAGCTACGCC 
AAATCCCTGC TGCATGGAAG ATGCTTTGAC AGGTGGTTTG ACAAGTCCAT CACCTTTGTT 
GTCTTCAAAA ACAGCAAGAT AGGCATAAAT GCAGAGCACT CCTGGGCGGA CGCGCCCGTC 
GTGGGCCATT TGTGGGAGTA TGTCATGGCC ACCGACGTCT TCCAGCTGGG TTACTCAGAG 
GATGGACACT GTAAAGGAGA CACCAACCCC AACATCCCTA AGCCCACAAG GCTACAATGG 
GACATTCCAG GAGAGTGCCA GGAGGTCATA GATGCATCCC TGAGCAGCGC CAGTCTTTTG 
GCAAATGATG TGGACCTGCA TTCCTTCCCA TTTGACTCTT TCGGCAAAGG CTTGATCAAG 
AAGTGCCGGA CGAGTCCCGA TGCCTTCATC CAGCTGGCGC TGCAGCTCGC ACATTACAAG 
GACATGGGCA AGTTCTGCCT CACATATGAG GCCTCCATGA CCCGGCTCTT CCGAGAAGGG 
AGGACAGAGA CTGTACGCTC CTGCACTATG GAGTCCTGCA ACTTTGTGCA GGCCATGATG 
GACCCCAAGT CAACGGCAGA GCAGAGGCTC AAGCTGTTCA AGATAGCTTG TGAGAAGCAC 
CAGCACCTGT ACCGCCTCGC CATGACGGGC GCCGGCATCG ACCGCCATCT CTTCTGCCTC 
TATGTGGTGT CCAAGTATCT TGCAGTCGAC TCACCTTTCC TGAAGGAGGT ATTGTCTGAG 
CCATGGAGGT TGTCTACGAG CCAGACTCCT CAGCAGCAGG TGGAGCTCTT TGACTTTGAG 
AAAAACCCTG ACTATGTGTC CTGTGGAGGG GGCTTTGGGC CGGTTGCCGA TGACGGCTAT 
GGTGTCTCCT ACATTATAGT GGGAGAGAAT TTTATCCACT TCCATATTTC TTCCAAGTTC 
TCTAGCCCTG AGACAGACTC ACACCGCTTT GGGAAGCACT TGAGACAAGC CATGATGGAC 
ATTATCACCT TGTTTGGCCT CACCATCAAT TCTAAAAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017444326.1
CDS	225..2546
Translation	MAEAHQAVAFQFTVTPDGIDLRLSHEALKQICLSGLHSWKKKFIRFKNGIITGVFPANPSSWLIVVVGVISSMHAKVDPSLGMIAKISRTLDTTGRMSSQTKNIVSGVLFGTGLWVAVIMTMRYSLKVLLSYHGWMFAEHGKMSRSTKIWMAMVKVLSGRKPMLYSFQTSLPRLPVPAVKDTVSRYLESVRPLMKEEDFQRMTALAQDFAVNLGPKLQWYLKLKSWWATNYVSDWWEEYIYLRGRGPLMVNSNYYAMEMLYITPTHIQAARAGNTIHAILLYRRTLDREELKPIRLLGSTIPLCSAQWERLFNTSRIPGEETDTIQHIKDSRHIVVYHRGRYFKVWLYHDGRLLRPRELEQQMQQILDDPSEPQPGEAKLAALTAADRVPWAKCRQTYFARGKNKQSLDAVEKAAFFVTLDESEQGYREEDPEASIDSYAKSLLHGRCFDRWFDKSITFVVFKNSKIGINAEHSWADAPVVGHLWEYVMATDVFQLGYSEDGHCKGDTNPNIPKPTRLQWDIPGECQEVIDASLSSASLLANDVDLHSFPFDSFGKGLIKKCRTSPDAFIQLALQLAHYKDMGKFCLTYEASMTRLFREGRTETVRSCTMESCNFVQAMMDPKSTAEQRLKLFKIACEKHQHLYRLAMTGAGIDRHLFCLYVVSKYLAVDSPFLKEVLSEPWRLSTSQTPQQQVELFDFEKNPDYVSCGGGFGPVADDGYGVSYIIVGENFIHFHISSKFSSPETDSHRFGKHLRQAMMDIITLFGLTINSKK

Target ORF information:

RefSeq Version	XM_017588837.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus carnitine palmitoyltransferase 1A (Cpt1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017588837.1

ORF Insert Sequence:

ATGGCAGAGG CTCACCAAGC TGTGGCCTTC CAGTTCACTG TCACCCCCGA TGGCATTGAC 
CTCCGCCTGA GCCACGAAGC CCTCAAACAG ATCTGCCTGT CGGGGCTGCA CTCCTGGAAG 
AAGAAGTTCA TCCGGTTCAA GAATGGCATC ATCACTGGTG TGTTCCCCGC GAATCCGTCC 
AGCTGGCTTA TCGTGGTGGT GGGTGTGATT TCATCCATGC ATGCCAAAGT GGACCCCTCC 
CTGGGCATGA TCGCAAAGAT CAGTCGGACC CTAGACACCA CTGGCCGAAT GTCAAGCCAG 
ACGAAGAACA TTGTGAGCGG CGTCCTCTTT GGTACAGGGC TCTGGGTGGC AGTCATCATG 
ACCATGCGCT ACTCGCTGAA GGTGCTGCTC TCCTACCACG GCTGGATGTT TGCAGAACAC 
GGCAAAATGA GCCGCAGCAC CAAGATCTGG ATGGCTATGG TCAAGGTCCT CTCAGGTCGG 
AAGCCCATGT TGTACAGCTT CCAGACGTCT CTGCCACGCC TGCCTGTCCC AGCTGTCAAA 
GATACTGTGA GCAGGTACCT GGAATCTGTG AGGCCACTGA TGAAGGAAGA AGACTTCCAG 
CGCATGACAG CACTGGCCCA GGATTTTGCT GTCAACCTCG GACCCAAATT GCAGTGGTAT 
TTGAAGCTAA AATCCTGGTG GGCCACAAAT TACGTGAGTG ACTGGTGGGA AGAATATATC 
TACCTGCGGG GCCGAGGGCC GCTCATGGTC AACAGCAACT ACTACGCCAT GGAGATGCTG 
TACATCACCC CAACCCATAT CCAGGCAGCG AGAGCTGGCA ACACCATCCA CGCCATACTG 
CTGTATCGTC GCACATTAGA CCGTGAGGAA CTCAAACCCA TTCGTCTTCT GGGATCCACC 
ATTCCACTCT GCTCGGCCCA GTGGGAGCGA CTCTTCAATA CTTCCCGGAT CCCTGGGGAA 
GAGACAGACA CCATCCAACA TATCAAGGAC AGCAGGCACA TTGTTGTGTA CCACAGAGGG 
CGGTACTTCA AGGTCTGGCT CTACCACGAT GGGAGGCTGC TGAGGCCCCG AGAGCTGGAG 
CAGCAGATGC AGCAGATCCT GGATGATCCC TCAGAGCCAC AGCCTGGGGA GGCCAAGCTG 
GCCGCCCTCA CTGCTGCAGA CAGAGTGCCC TGGGCAAAGT GTCGGCAGAC CTATTTTGCA 
CGAGGGAAAA ATAAGCAGTC CCTGGATGCG GTGGAAAAGG CAGCGTTCTT CGTGACGTTG 
GACGAATCGG AGCAGGGATA CAGAGAGGAG GATCCTGAGG CATCCATCGA CAGCTACGCC 
AAATCCCTGC TGCATGGAAG ATGCTTTGAC AGGTGGTTTG ACAAGTCCAT CACCTTTGTT 
GTCTTCAAAA ACAGCAAGAT AGGCATAAAT GCAGAGCACT CCTGGGCGGA CGCGCCCGTC 
GTGGGCCATT TGTGGGAGTA TGTCATGGCC ACCGACGTCT TCCAGCTGGG TTACTCAGAG 
GATGGACACT GTAAAGGAGA CACCAACCCC AACATCCCTA AGCCCACAAG GCTACAATGG 
GACATTCCAG GAGAGTGCCA GGAGGTCATA GATGCATCCC TGAGCAGCGC CAGTCTTTTG 
GCAAATGATG TGGACCTGCA TTCCTTCCCA TTTGACTCTT TCGGCAAAGG CTTGATCAAG 
AAGTGCCGGA CGAGTCCCGA TGCCTTCATC CAGCTGGCGC TGCAGCTCGC ACATTACAAG 
GACATGGGCA AGTTCTGCCT CACATATGAG GCCTCCATGA CCCGGCTCTT CCGAGAAGGG 
AGGACAGAGA CTGTACGCTC CTGCACTATG GAGTCCTGCA ACTTTGTGCA GGCCATGATG 
GACCCCAAGT CAACGGCAGA GCAGAGGCTC AAGCTGTTCA AGATAGCTTG TGAGAAGCAC 
CAGCACCTGT ACCGCCTCGC CATGACGGGC GCCGGCATCG ACCGCCATCT CTTCTGCCTC 
TATGTGGTGT CCAAGTATCT TGCAGTCGAC TCACCTTTCC TGAAGGAGGT ATTGTCTGAG 
CCATGGAGGT TGTCTACGAG CCAGACTCCT CAGCAGCAGG TGGAGCTCTT TGACTTTGAG 
AAAAACCCTG ACTATGTGTC CTGTGGAGGG GGCTTTGGGC CGGTTGCCGA TGACGGCTAT 
GGTGTCTCCT ACATTATAGT GGGAGAGAAT TTTATCCACT TCCATATTTC TTCCAAGTTC 
TCTAGCCCTG AGACAGACTC ACACCGCTTT GGGAAGCACT TGAGACAAGC CATGATGGAC 
ATTATCACCT TGTTTGGCCT CACCATCAAT TCTAAAAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cpt1a ( XM_017588838.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017444327.1 Rattus norvegicus carnitine palmitoyltransferase 1A (Cpt1a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa56414
Clone ID Related Accession (Same CDS sequence)	XM_017588838.1
Accession Version	XM_017588838.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2034bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	carnitine O-palmitoyltransferase 1, liver isoform isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAGCC AGACGAAGAA CATTGTGAGC GGCGTCCTCT TTGGTACAGG GCTCTGGGTG 
GCAGTCATCA TGACCATGCG CTACTCGCTG AAGGTGCTGC TCTCCTACCA CGGCTGGATG 
TTTGCAGAAC ACGGCAAAAT GAGCCGCAGC ACCAAGATCT GGATGGCTAT GGTCAAGGTC 
CTCTCAGGTC GGAAGCCCAT GTTGTACAGC TTCCAGACGT CTCTGCCACG CCTGCCTGTC 
CCAGCTGTCA AAGATACTGT GAGCAGGTAC CTGGAATCTG TGAGGCCACT GATGAAGGAA 
GAAGACTTCC AGCGCATGAC AGCACTGGCC CAGGATTTTG CTGTCAACCT CGGACCCAAA 
TTGCAGTGGT ATTTGAAGCT AAAATCCTGG TGGGCCACAA ATTACGTGAG TGACTGGTGG 
GAAGAATATA TCTACCTGCG GGGCCGAGGG CCGCTCATGG TCAACAGCAA CTACTACGCC 
ATGGAGATGC TGTACATCAC CCCAACCCAT ATCCAGGCAG CGAGAGCTGG CAACACCATC 
CACGCCATAC TGCTGTATCG TCGCACATTA GACCGTGAGG AACTCAAACC CATTCGTCTT 
CTGGGATCCA CCATTCCACT CTGCTCGGCC CAGTGGGAGC GACTCTTCAA TACTTCCCGG 
ATCCCTGGGG AAGAGACAGA CACCATCCAA CATATCAAGG ACAGCAGGCA CATTGTTGTG 
TACCACAGAG GGCGGTACTT CAAGGTCTGG CTCTACCACG ATGGGAGGCT GCTGAGGCCC 
CGAGAGCTGG AGCAGCAGAT GCAGCAGATC CTGGATGATC CCTCAGAGCC ACAGCCTGGG 
GAGGCCAAGC TGGCCGCCCT CACTGCTGCA GACAGAGTGC CCTGGGCAAA GTGTCGGCAG 
ACCTATTTTG CACGAGGGAA AAATAAGCAG TCCCTGGATG CGGTGGAAAA GGCAGCGTTC 
TTCGTGACGT TGGACGAATC GGAGCAGGGA TACAGAGAGG AGGATCCTGA GGCATCCATC 
GACAGCTACG CCAAATCCCT GCTGCATGGA AGATGCTTTG ACAGGTGGTT TGACAAGTCC 
ATCACCTTTG TTGTCTTCAA AAACAGCAAG ATAGGCATAA ATGCAGAGCA CTCCTGGGCG 
GACGCGCCCG TCGTGGGCCA TTTGTGGGAG TATGTCATGG CCACCGACGT CTTCCAGCTG 
GGTTACTCAG AGGATGGACA CTGTAAAGGA GACACCAACC CCAACATCCC TAAGCCCACA 
AGGCTACAAT GGGACATTCC AGGAGAGTGC CAGGAGGTCA TAGATGCATC CCTGAGCAGC 
GCCAGTCTTT TGGCAAATGA TGTGGACCTG CATTCCTTCC CATTTGACTC TTTCGGCAAA 
GGCTTGATCA AGAAGTGCCG GACGAGTCCC GATGCCTTCA TCCAGCTGGC GCTGCAGCTC 
GCACATTACA AGGACATGGG CAAGTTCTGC CTCACATATG AGGCCTCCAT GACCCGGCTC 
TTCCGAGAAG GGAGGACAGA GACTGTACGC TCCTGCACTA TGGAGTCCTG CAACTTTGTG 
CAGGCCATGA TGGACCCCAA GTCAACGGCA GAGCAGAGGC TCAAGCTGTT CAAGATAGCT 
TGTGAGAAGC ACCAGCACCT GTACCGCCTC GCCATGACGG GCGCCGGCAT CGACCGCCAT 
CTCTTCTGCC TCTATGTGGT GTCCAAGTAT CTTGCAGTCG ACTCACCTTT CCTGAAGGAG 
GTATTGTCTG AGCCATGGAG GTTGTCTACG AGCCAGACTC CTCAGCAGCA GGTGGAGCTC 
TTTGACTTTG AGAAAAACCC TGACTATGTG TCCTGTGGAG GGGGCTTTGG GCCGGTTGCC 
GATGACGGCT ATGGTGTCTC CTACATTATA GTGGGAGAGA ATTTTATCCA CTTCCATATT 
TCTTCCAAGT TCTCTAGCCC TGAGACAGAC TCACACCGCT TTGGGAAGCA CTTGAGACAA 
GCCATGATGG ACATTATCAC CTTGTTTGGC CTCACCATCA ATTCTAAAAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017444327.1
CDS	86..2119
Translation	MSSQTKNIVSGVLFGTGLWVAVIMTMRYSLKVLLSYHGWMFAEHGKMSRSTKIWMAMVKVLSGRKPMLYSFQTSLPRLPVPAVKDTVSRYLESVRPLMKEEDFQRMTALAQDFAVNLGPKLQWYLKLKSWWATNYVSDWWEEYIYLRGRGPLMVNSNYYAMEMLYITPTHIQAARAGNTIHAILLYRRTLDREELKPIRLLGSTIPLCSAQWERLFNTSRIPGEETDTIQHIKDSRHIVVYHRGRYFKVWLYHDGRLLRPRELEQQMQQILDDPSEPQPGEAKLAALTAADRVPWAKCRQTYFARGKNKQSLDAVEKAAFFVTLDESEQGYREEDPEASIDSYAKSLLHGRCFDRWFDKSITFVVFKNSKIGINAEHSWADAPVVGHLWEYVMATDVFQLGYSEDGHCKGDTNPNIPKPTRLQWDIPGECQEVIDASLSSASLLANDVDLHSFPFDSFGKGLIKKCRTSPDAFIQLALQLAHYKDMGKFCLTYEASMTRLFREGRTETVRSCTMESCNFVQAMMDPKSTAEQRLKLFKIACEKHQHLYRLAMTGAGIDRHLFCLYVVSKYLAVDSPFLKEVLSEPWRLSTSQTPQQQVELFDFEKNPDYVSCGGGFGPVADDGYGVSYIIVGENFIHFHISSKFSSPETDSHRFGKHLRQAMMDIITLFGLTINSKK

Target ORF information:

RefSeq Version	XM_017588838.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus carnitine palmitoyltransferase 1A (Cpt1a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017588838.1

ORF Insert Sequence:

ATGTCAAGCC AGACGAAGAA CATTGTGAGC GGCGTCCTCT TTGGTACAGG GCTCTGGGTG 
GCAGTCATCA TGACCATGCG CTACTCGCTG AAGGTGCTGC TCTCCTACCA CGGCTGGATG 
TTTGCAGAAC ACGGCAAAAT GAGCCGCAGC ACCAAGATCT GGATGGCTAT GGTCAAGGTC 
CTCTCAGGTC GGAAGCCCAT GTTGTACAGC TTCCAGACGT CTCTGCCACG CCTGCCTGTC 
CCAGCTGTCA AAGATACTGT GAGCAGGTAC CTGGAATCTG TGAGGCCACT GATGAAGGAA 
GAAGACTTCC AGCGCATGAC AGCACTGGCC CAGGATTTTG CTGTCAACCT CGGACCCAAA 
TTGCAGTGGT ATTTGAAGCT AAAATCCTGG TGGGCCACAA ATTACGTGAG TGACTGGTGG 
GAAGAATATA TCTACCTGCG GGGCCGAGGG CCGCTCATGG TCAACAGCAA CTACTACGCC 
ATGGAGATGC TGTACATCAC CCCAACCCAT ATCCAGGCAG CGAGAGCTGG CAACACCATC 
CACGCCATAC TGCTGTATCG TCGCACATTA GACCGTGAGG AACTCAAACC CATTCGTCTT 
CTGGGATCCA CCATTCCACT CTGCTCGGCC CAGTGGGAGC GACTCTTCAA TACTTCCCGG 
ATCCCTGGGG AAGAGACAGA CACCATCCAA CATATCAAGG ACAGCAGGCA CATTGTTGTG 
TACCACAGAG GGCGGTACTT CAAGGTCTGG CTCTACCACG ATGGGAGGCT GCTGAGGCCC 
CGAGAGCTGG AGCAGCAGAT GCAGCAGATC CTGGATGATC CCTCAGAGCC ACAGCCTGGG 
GAGGCCAAGC TGGCCGCCCT CACTGCTGCA GACAGAGTGC CCTGGGCAAA GTGTCGGCAG 
ACCTATTTTG CACGAGGGAA AAATAAGCAG TCCCTGGATG CGGTGGAAAA GGCAGCGTTC 
TTCGTGACGT TGGACGAATC GGAGCAGGGA TACAGAGAGG AGGATCCTGA GGCATCCATC 
GACAGCTACG CCAAATCCCT GCTGCATGGA AGATGCTTTG ACAGGTGGTT TGACAAGTCC 
ATCACCTTTG TTGTCTTCAA AAACAGCAAG ATAGGCATAA ATGCAGAGCA CTCCTGGGCG 
GACGCGCCCG TCGTGGGCCA TTTGTGGGAG TATGTCATGG CCACCGACGT CTTCCAGCTG 
GGTTACTCAG AGGATGGACA CTGTAAAGGA GACACCAACC CCAACATCCC TAAGCCCACA 
AGGCTACAAT GGGACATTCC AGGAGAGTGC CAGGAGGTCA TAGATGCATC CCTGAGCAGC 
GCCAGTCTTT TGGCAAATGA TGTGGACCTG CATTCCTTCC CATTTGACTC TTTCGGCAAA 
GGCTTGATCA AGAAGTGCCG GACGAGTCCC GATGCCTTCA TCCAGCTGGC GCTGCAGCTC 
GCACATTACA AGGACATGGG CAAGTTCTGC CTCACATATG AGGCCTCCAT GACCCGGCTC 
TTCCGAGAAG GGAGGACAGA GACTGTACGC TCCTGCACTA TGGAGTCCTG CAACTTTGTG 
CAGGCCATGA TGGACCCCAA GTCAACGGCA GAGCAGAGGC TCAAGCTGTT CAAGATAGCT 
TGTGAGAAGC ACCAGCACCT GTACCGCCTC GCCATGACGG GCGCCGGCAT CGACCGCCAT 
CTCTTCTGCC TCTATGTGGT GTCCAAGTAT CTTGCAGTCG ACTCACCTTT CCTGAAGGAG 
GTATTGTCTG AGCCATGGAG GTTGTCTACG AGCCAGACTC CTCAGCAGCA GGTGGAGCTC 
TTTGACTTTG AGAAAAACCC TGACTATGTG TCCTGTGGAG GGGGCTTTGG GCCGGTTGCC 
GATGACGGCT ATGGTGTCTC CTACATTATA GTGGGAGAGA ATTTTATCCA CTTCCATATT 
TCTTCCAAGT TCTCTAGCCC TGAGACAGAC TCACACCGCT TTGGGAAGCA CTTGAGACAA 
GCCATGATGG ACATTATCAC CTTGTTTGGC CTCACCATCA ATTCTAAAAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Obesity-alleviating potential of asiatic acid and its effects on ACC1, UCP2, and CPT1 mRNA expression in high fat diet-induced obese Sprague-Dawley rats.
Molecular and cellular biochemistry(2017 Oct)
Rameshreddy P,Uddandrao VVS,Brahmanaidu P,Vadivukkarasi S,Ravindarnaik R,Suresh P,Swapna K,Kalaivani A,Parvathi P,Tamilmani P,Saravanan G

Examination of carnitine palmitoyltransferase 1 abundance in white adipose tissue: implications in obesity research.
American journal of physiology. Regulatory, integrative and comparative physiology312(5)R816-R820(2017 May)
Warfel JD,Vandanmagsar B,Dubuisson OS,Hodgeson SM,Elks CM,Ravussin E,Mynatt RL

Type of supplemented simple sugar, not merely calorie intake, determines adverse effects on metabolism and aortic function in female rats.
American journal of physiology. Heart and circulatory physiology312(2)H289-H304(2017 Feb)
Sang?esa G,Shaligram S,Akther F,Roglans N,Laguna JC,Rahimian R,Alegret M

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Cpt1a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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