MAP3K12 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	MAP3K12
Entrez Gene ID	505427
Full Name	mitogen-activated protein kinase kinase kinase 12
General protein information	Preferred Names mitogen-activated protein kinase kinase kinase 12 Names mitogen-activated protein kinase kinase kinase 12
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005206141.3	XP_005206198.1	mitogen-activated protein kinase kinase kinase 12 isoform X1
XM_024991461.1	XP_024847229.1	mitogen-activated protein kinase kinase kinase 12 isoform X1
NM_001205291.1	NP_001192220.1	mitogen-activated protein kinase kinase kinase 12
XM_005206141.4	XP_005206198.1	mitogen-activated protein kinase kinase kinase 12 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MAP3K12	XP_509099.3
Bos taurus (cattle)	MAP3K12	NP_001192220.1
Homo sapiens (human)	MAP3K12	NP_001180440.1
Canis lupus familiaris (dog)	MAP3K12	XP_848815.1
Danio rerio (zebrafish)	map3k12	NP_996977.1
Mus musculus (house mouse)	Map3k12	NP_001157115.1
Rattus norvegicus (Norway rat)	Map3k12	NP_037187.1
	MAP3K12	XP_001105474.1
Xenopus tropicalis (tropical clawed frog)	map3k12	XP_004911999.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Analysis of bovine mammary gland EST and functional annotation of the Bos taurus gene index.
Sonstegard TS, Capuco AV, White J, Van Tassell CP, Connor EE, Cho J, Sultana R, Shade L, Wray JE, Wells KD, Quackenbush J
Mammalian genome : official journal of the International Mammalian Genome Society13(7)373-9(2002 Jul)

Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries and construction of a gene index for cattle.
Smith TP, Grosse WM, Freking BA, Roberts AJ, Stone RT, Casas E, Wray JE, White J, Cho J, Fahrenkrug SC, Bennett GL, Heaton MP, Laegreid WW, Rohrer GA, Chitko-McKown CG, Pertea G, Holt I, Karamycheva S, Liang F, Quackenbush J, Keele JW
Genome research11(4)626-30(2001 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MAP3K12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAP3K12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa36674	NM_001205291.1 Latest version!	Bos taurus mitogen-activated protein kinase kinase kinase 12 (MAP3K12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa19101	XM_024991461.1 Latest version!	Bos taurus mitogen-activated protein kinase kinase kinase 12 (MAP3K12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa19101	XM_005206141.4 Latest version!	Bos taurus mitogen-activated protein kinase kinase kinase 12 (MAP3K12), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
View Old Accession Versions >>
OBa19101	XM_005206141.3	Bos taurus mitogen-activated protein kinase kinase kinase 12 (MAP3K12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MAP3K12 ( NM_001205291.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001192220.1 Bos taurus mitogen-activated protein kinase kinase kinase 12 (MAP3K12), mRNA.

Price & Availability↑

CloneID	OBa36674
Clone ID Related Accession (Same CDS sequence)	NM_001205291.1
Accession Version	NM_001205291.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2583bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-13
Organism	Bos taurus(cattle)
Product	mitogen-activated protein kinase kinase kinase 12
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC151621.1, DV908045.1, DN283480.1, BE845433.1, EH173220.1, BE663505.1, DN525550.1 and BE756090.1. On Apr 14, 2011 this sequence version replaced XM_002687238.1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145415, SAMN03145422 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCTGCC TCCATGAGAC CCGCACACCC TCCCCTTCCT TTGGGGGTTT CGTGTCCACC 
CTAAGCGAGG CATCCATGCG CAAGCTGGAC CCAGACACTT CCGACTGCAC CCCCGAGAAG 
GACCTGACGC CCACCCATGT CCTGCAGCTC CACGAGCAGG ATGCAGGGGG CCCAGGGGGA 
GCCACTGGGT CCCCTGAGAG TCGGGCGTCC AGGGTCCGAG CGGATGAGGT ACGGCTCCAG 
TGCCAGAGCG GCAGCGGCTT CCTGGAGGGC CTCTTCGGCT GCCTGCGCCC TGTGTGGACC 
ATGATCGGCA AAGCCTACTC CACGGAGCAC AAGCAGCAGC AGGAAGACCT TTGGGAGGTC 
CCCTTTGAGG AAATCCTGGA CCTGCAGTGG GTGGGCTCAG GGGCCCAGGG CGCCGTCTTC 
CTGGGGCGCT TCCATGGGGA GGAGGTGGCT GTGAAGAAGG TGCGGGACCT GAAGGAAACT 
GACATCAAGC ACCTGCGAAA GCTGAAGCAC CCAAACATCA TCACCTTCAA GGGTGTGTGC 
ACCCAGGCTC CCTGCTACTG CATCCTCATG GAGTTCTGCG CCCAGGGCCA GCTGTACGAG 
GTGCTGCGGG CTGGCCGCCC CGTCACGCCC TCCTTGCTGG TTGACTGGTC CATGGGCATC 
GCCGGCGGCA TGAACTACCT GCACCTGCAC AAGATTATCC ATCGGGACCT CAAGTCCCCC 
AACATGCTAA TCACCTACGA CGATGTGGTG AAGATCTCCG ATTTTGGCAC TTCCAAGGAG 
CTGAGTGACA AGAGCACCAA GATGTCCTTT GCAGGGACAG TAGCCTGGAT GGCCCCTGAA 
GTGATCCGCA ACGAACCTGT GTCTGAGAAG GTTGACATCT GGTCCTTTGG CGTGGTGCTG 
TGGGAGCTGC TGACTGGTGA GATCCCCTAC AAAGACGTAG ATTCCTCAGC CATCATCTGG 
GGTGTGGGAA GCAACAGTCT CCATCTACCT GTGCCCTCCA GCTGCCCAGA CGGCTTCAAA 
ATCCTGCTTC GCCAGTGCTG GAACAGCAAA CCACGAAACC GCCCATCATT CCGACAGATC 
CTGCTGCATC TGGACATTGC CTCAGCCGAC GTACTCTCCA CACCCCAGGA GACTTACTTT 
AAGTCCCAGG CAGAGTGGCG TGAAGAGGTA AAGCTGCATT TTGAAAAAAT TAAGTCAGAA 
GGGACCTGTC TGCACCGCCT AGAAGAGGAG CTGGTGATGC GGAGGAGGGA GGAGCTCAGA 
CATGCCCTGG ACATTAGGGA ACACTATGAG CGGAAACTGG AGAGAGCCAA CAACCTGTAC 
ATGGAACTTA ATGCCCTTAT GTTGCAGCTG GAGCTGAAGG AACGGGAGCT ACTCAGGAGG 
GAGCAAGCTT TAGAGCGGCG GTGTCCAGGT CTGCTGAAGT CACACCCTTC CCGGGGCCTG 
CTGCACGGGA ACACGATGGA GAAACTGATC AAGAAGAGGA ATGTCCCACA GAAACTGTCG 
CCCCACAGCA AAAGGCCAGA TATCCTCAAG ACTGAGTCTT TGCTACCCAA ACTAGATGCA 
GCCCTGAGTG GGGTGGGGCT TCCTGGGTGT GCTAAGGGCC CCCCCTCACC AGGACGGAGT 
CGCCGTGGCA AGACTCGTCA CCGCAAGGCC AGCGCCAAGG GCAGCTGTGG GGACCTGCCT 
GGGCTTCGTG CAGCTGTGCC ACCCCATGAA CCTGGGGGAC CAGGAAGCCC CGTGGGGCTA 
GGCGGGGGAC CCTCAGCCTG GGAAGCCTGC CCTCCAGCCC TCCGTGGGCT CCACCATGAT 
CTCCTGCTCC GAAAGATGTC TTCATCATCG CCAGATCTGC TGTCAGCAGC ATTGGGAGCC 
CGGGGCCGGG GGGCTACAGG GGGAGCTGGG GATCCTGGCT CACCACCTCC AGCCCGGGGA 
GACACGCCCC CAAGCGAGGG CTCAGCACCT GGCTCCACCA GCCCGGATTC ACCAGGGGGA 
GCCAAAGGGG AGCCGCCTCC ACCAGTAGGA CCTGGTGACA GTGTAGGGCT GCTGGGAACT 
GGAAGGGAAG GGACGGCGGG ACGGGGAGGA AGCCGGGCTG GGTCCCAGCA CTTGACCCCA 
GCCGCACTGC TGTACAGGGC TGCTGTCACC CGAAGTCAGA AACGCGGCAT CTCATCAGAG 
GAAGAGGAAG GAGAGGTGGA CAGTGAAGTG GAACTGGCCT CAAGCCAGAG GTGGCCTCAG 
GGCCTGAAAA TGCGCCAGTC ACTATCTACC TTCAGCTCAG AGAATCCATC GGATGGGGAG 
GAGGGCACAG CTAGTGAGCC TTCCCCCAGT GGCACACCTG AAGTTGGCAG TACCAACACA 
GATGAGCGGC CGGATGAACG GTCTGATGAC ATGTGCTCGC AGGGCTCAGA AATCCCACTG 
GACCCACCAG CTTCTGAGGT GGTTACTGGC CCAGAACCCA GCTCCTTGCC CGTCCCACAC 
CACGACCTAC TCAGAGGGGA GCAGGGCCCT CCCAACCCTG AGGACTCAGA CTGTGACAGC 
ACTGAACTGG ACAACGCCAA CAGCGGTGAA GCCTTGCGGC CCCCAGCCTC CCTCCCTCCG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001192220.1
CDS	40..2622
Translation	MACLHETRTPSPSFGGFVSTLSEASMRKLDPDTSDCTPEKDLTPTHVLQLHEQDAGGPGGATGSPESRASRVRADEVRLQCQSGSGFLEGLFGCLRPVWTMIGKAYSTEHKQQQEDLWEVPFEEILDLQWVGSGAQGAVFLGRFHGEEVAVKKVRDLKETDIKHLRKLKHPNIITFKGVCTQAPCYCILMEFCAQGQLYEVLRAGRPVTPSLLVDWSMGIAGGMNYLHLHKIIHRDLKSPNMLITYDDVVKISDFGTSKELSDKSTKMSFAGTVAWMAPEVIRNEPVSEKVDIWSFGVVLWELLTGEIPYKDVDSSAIIWGVGSNSLHLPVPSSCPDGFKILLRQCWNSKPRNRPSFRQILLHLDIASADVLSTPQETYFKSQAEWREEVKLHFEKIKSEGTCLHRLEEELVMRRREELRHALDIREHYERKLERANNLYMELNALMLQLELKERELLRREQALERRCPGLLKSHPSRGLLHGNTMEKLIKKRNVPQKLSPHSKRPDILKTESLLPKLDAALSGVGLPGCAKGPPSPGRSRRGKTRHRKASAKGSCGDLPGLRAAVPPHEPGGPGSPVGLGGGPSAWEACPPALRGLHHDLLLRKMSSSSPDLLSAALGARGRGATGGAGDPGSPPPARGDTPPSEGSAPGSTSPDSPGGAKGEPPPPVGPGDSVGLLGTGREGTAGRGGSRAGSQHLTPAALLYRAAVTRSQKRGISSEEEEGEVDSEVELASSQRWPQGLKMRQSLSTFSSENPSDGEEGTASEPSPSGTPEVGSTNTDERPDERSDDMCSQGSEIPLDPPASEVVTGPEPSSLPVPHHDLLRGEQGPPNPEDSDCDSTELDNANSGEALRPPASLPP

Target ORF information:

RefSeq Version	NM_001205291.1
Organism	Bos taurus(cattle)
Definition	Bos taurus mitogen-activated protein kinase kinase kinase 12 (MAP3K12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001205291.1

ORF Insert Sequence:

ATGGCCTGCC TCCATGAGAC CCGCACACCC TCCCCTTCCT TTGGGGGTTT CGTGTCCACC 
CTAAGCGAGG CATCCATGCG CAAGCTGGAC CCAGACACTT CCGACTGCAC CCCCGAGAAG 
GACCTGACGC CCACCCATGT CCTGCAGCTC CACGAGCAGG ATGCAGGGGG CCCAGGGGGA 
GCCACTGGGT CCCCTGAGAG TCGGGCGTCC AGGGTCCGAG CGGATGAGGT ACGGCTCCAG 
TGCCAGAGCG GCAGCGGCTT CCTGGAGGGC CTCTTCGGCT GCCTGCGCCC TGTGTGGACC 
ATGATCGGCA AAGCCTACTC CACGGAGCAC AAGCAGCAGC AGGAAGACCT TTGGGAGGTC 
CCCTTTGAGG AAATCCTGGA CCTGCAGTGG GTGGGCTCAG GGGCCCAGGG CGCCGTCTTC 
CTGGGGCGCT TCCATGGGGA GGAGGTGGCT GTGAAGAAGG TGCGGGACCT GAAGGAAACT 
GACATCAAGC ACCTGCGAAA GCTGAAGCAC CCAAACATCA TCACCTTCAA GGGTGTGTGC 
ACCCAGGCTC CCTGCTACTG CATCCTCATG GAGTTCTGCG CCCAGGGCCA GCTGTACGAG 
GTGCTGCGGG CTGGCCGCCC CGTCACGCCC TCCTTGCTGG TTGACTGGTC CATGGGCATC 
GCCGGCGGCA TGAACTACCT GCACCTGCAC AAGATTATCC ATCGGGACCT CAAGTCCCCC 
AACATGCTAA TCACCTACGA CGATGTGGTG AAGATCTCCG ATTTTGGCAC TTCCAAGGAG 
CTGAGTGACA AGAGCACCAA GATGTCCTTT GCAGGGACAG TAGCCTGGAT GGCCCCTGAA 
GTGATCCGCA ACGAACCTGT GTCTGAGAAG GTTGACATCT GGTCCTTTGG CGTGGTGCTG 
TGGGAGCTGC TGACTGGTGA GATCCCCTAC AAAGACGTAG ATTCCTCAGC CATCATCTGG 
GGTGTGGGAA GCAACAGTCT CCATCTACCT GTGCCCTCCA GCTGCCCAGA CGGCTTCAAA 
ATCCTGCTTC GCCAGTGCTG GAACAGCAAA CCACGAAACC GCCCATCATT CCGACAGATC 
CTGCTGCATC TGGACATTGC CTCAGCCGAC GTACTCTCCA CACCCCAGGA GACTTACTTT 
AAGTCCCAGG CAGAGTGGCG TGAAGAGGTA AAGCTGCATT TTGAAAAAAT TAAGTCAGAA 
GGGACCTGTC TGCACCGCCT AGAAGAGGAG CTGGTGATGC GGAGGAGGGA GGAGCTCAGA 
CATGCCCTGG ACATTAGGGA ACACTATGAG CGGAAACTGG AGAGAGCCAA CAACCTGTAC 
ATGGAACTTA ATGCCCTTAT GTTGCAGCTG GAGCTGAAGG AACGGGAGCT ACTCAGGAGG 
GAGCAAGCTT TAGAGCGGCG GTGTCCAGGT CTGCTGAAGT CACACCCTTC CCGGGGCCTG 
CTGCACGGGA ACACGATGGA GAAACTGATC AAGAAGAGGA ATGTCCCACA GAAACTGTCG 
CCCCACAGCA AAAGGCCAGA TATCCTCAAG ACTGAGTCTT TGCTACCCAA ACTAGATGCA 
GCCCTGAGTG GGGTGGGGCT TCCTGGGTGT GCTAAGGGCC CCCCCTCACC AGGACGGAGT 
CGCCGTGGCA AGACTCGTCA CCGCAAGGCC AGCGCCAAGG GCAGCTGTGG GGACCTGCCT 
GGGCTTCGTG CAGCTGTGCC ACCCCATGAA CCTGGGGGAC CAGGAAGCCC CGTGGGGCTA 
GGCGGGGGAC CCTCAGCCTG GGAAGCCTGC CCTCCAGCCC TCCGTGGGCT CCACCATGAT 
CTCCTGCTCC GAAAGATGTC TTCATCATCG CCAGATCTGC TGTCAGCAGC ATTGGGAGCC 
CGGGGCCGGG GGGCTACAGG GGGAGCTGGG GATCCTGGCT CACCACCTCC AGCCCGGGGA 
GACACGCCCC CAAGCGAGGG CTCAGCACCT GGCTCCACCA GCCCGGATTC ACCAGGGGGA 
GCCAAAGGGG AGCCGCCTCC ACCAGTAGGA CCTGGTGACA GTGTAGGGCT GCTGGGAACT 
GGAAGGGAAG GGACGGCGGG ACGGGGAGGA AGCCGGGCTG GGTCCCAGCA CTTGACCCCA 
GCCGCACTGC TGTACAGGGC TGCTGTCACC CGAAGTCAGA AACGCGGCAT CTCATCAGAG 
GAAGAGGAAG GAGAGGTGGA CAGTGAAGTG GAACTGGCCT CAAGCCAGAG GTGGCCTCAG 
GGCCTGAAAA TGCGCCAGTC ACTATCTACC TTCAGCTCAG AGAATCCATC GGATGGGGAG 
GAGGGCACAG CTAGTGAGCC TTCCCCCAGT GGCACACCTG AAGTTGGCAG TACCAACACA 
GATGAGCGGC CGGATGAACG GTCTGATGAC ATGTGCTCGC AGGGCTCAGA AATCCCACTG 
GACCCACCAG CTTCTGAGGT GGTTACTGGC CCAGAACCCA GCTCCTTGCC CGTCCCACAC 
CACGACCTAC TCAGAGGGGA GCAGGGCCCT CCCAACCCTG AGGACTCAGA CTGTGACAGC 
ACTGAACTGG ACAACGCCAA CAGCGGTGAA GCCTTGCGGC CCCCAGCCTC CCTCCCTCCG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K12 ( XM_005206141.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005206198.1 Bos taurus mitogen-activated protein kinase kinase kinase 12 (MAP3K12), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa19101
Clone ID Related Accession (Same CDS sequence)	XM_005206141.3 , XM_024991461.1 , XM_005206141.4
Accession Version	XM_005206141.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2682bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	mitogen-activated protein kinase kinase kinase 12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103918.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005206141.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTGCC TCCATGAGAC CCGCACACCC TCCCCTTCCT TTGGGGGTTT CGTGTCCACC 
CTAAGCGAGG CATCCATGCG CAAGCTGGAC CCAGACACTT CCGACTGCAC CCCCGAGAAG 
GACCTGACGC CCACCCAGTG TGTACTCCGA GACGTAGTGC CACTCGGGGG TCAGGGCGGG 
GGCGGGCCCA GCCCCTCCCC AGGTGGAGAG CCGCCCCCTG AGCCTTTTGC CAACAGTGTC 
CTGCAGCTCC ACGAGCAGGA TGCAGGGGGC CCAGGGGGAG CCACTGGGTC CCCTGAGAGT 
CGGGCGTCCA GGGTCCGAGC GGATGAGGTA CGGCTCCAGT GCCAGAGCGG CAGCGGCTTC 
CTGGAGGGCC TCTTCGGCTG CCTGCGCCCT GTGTGGACCA TGATCGGCAA AGCCTACTCC 
ACGGAGCACA AGCAGCAGCA GGAAGACCTT TGGGAGGTCC CCTTTGAGGA AATCCTGGAC 
CTGCAGTGGG TGGGCTCAGG GGCCCAGGGC GCCGTCTTCC TGGGGCGCTT CCATGGGGAG 
GAGGTGGCTG TGAAGAAGGT GCGGGACCTG AAGGAAACTG ACATCAAGCA CCTGCGAAAG 
CTGAAGCACC CAAACATCAT CACCTTCAAG GGTGTGTGCA CCCAGGCTCC CTGCTACTGC 
ATCCTCATGG AGTTCTGCGC CCAGGGCCAG CTGTACGAGG TGCTGCGGGC TGGCCGCCCC 
GTCACGCCCT CCTTGCTGGT TGACTGGTCC ATGGGCATCG CCGGCGGCAT GAACTACCTG 
CACCTGCACA AGATTATCCA TCGGGACCTC AAGTCCCCCA ACATGCTAAT CACCTACGAC 
GATGTGGTGA AGATCTCCGA TTTTGGCACT TCCAAGGAGC TGAGTGACAA GAGCACCAAG 
ATGTCCTTTG CAGGGACAGT AGCCTGGATG GCCCCTGAAG TGATCCGCAA CGAACCTGTG 
TCTGAGAAGG TTGACATCTG GTCCTTTGGC GTGGTGCTGT GGGAGCTGCT GACTGGTGAG 
ATCCCCTACA AAGACGTAGA TTCCTCAGCC ATCATCTGGG GTGTGGGAAG CAACAGTCTC 
CATCTACCTG TGCCCTCCAG CTGCCCAGAC GGCTTCAAAA TCCTGCTTCG CCAGTGCTGG 
AACAGCAAAC CACGAAACCG CCCATCATTC CGACAGATCC TGCTGCATCT GGACATTGCC 
TCAGCCGACG TACTCTCCAC ACCCCAGGAG ACTTACTTTA AGTCCCAGGC AGAGTGGCGT 
GAAGAGGTAA AGCTGCATTT TGAAAAAATT AAGTCAGAAG GGACCTGTCT GCACCGCCTA 
GAAGAGGAGC TGGTGATGCG GAGGAGGGAG GAGCTCAGAC ATGCCCTGGA CATTAGGGAA 
CACTATGAGC GGAAACTGGA GAGAGCCAAC AACCTGTACA TGGAACTTAA TGCCCTTATG 
TTGCAGCTGG AGCTGAAGGA ACGGGAGCTA CTCAGGAGGG AGCAAGCTTT AGAGCGGCGG 
TGTCCAGGTC TGCTGAAGTC ACACCCTTCC CGGGGCCTGC TGCACGGGAA CACGATGGAG 
AAACTGATCA AGAAGAGGAA TGTCCCACAG AAACTGTCGC CCCACAGCAA AAGGCCAGAT 
ATCCTCAAGA CTGAGTCTTT GCTACCCAAA CTAGATGCAG CCCTGAGTGG GGTGGGGCTT 
CCTGGGTGTG CTAAGGGCCC CCCCTCACCA GGACGGAGTC GCCGTGGCAA GACTCGTCAC 
CGCAAGGCCA GCGCCAAGGG CAGCTGTGGG GACCTGCCTG GGCTTCGTGC AGCTGTGCCA 
CCCCATGAAC CTGGGGGACC AGGAAGCCCC GTGGGGCTAG GCGGGGGACC CTCAGCCTGG 
GAAGCCTGCC CTCCAGCCCT CCGTGGGCTC CACCATGATC TCCTGCTCCG AAAGATGTCT 
TCATCATCGC CAGATCTGCT GTCAGCAGCA TTGGGAGCCC GGGGCCGGGG GGCTACAGGG 
GGAGCTGGGG ATCCTGGCTC ACCACCTCCA GCCCGGGGAG ACACGCCCCC AAGCGAGGGC 
TCAGCACCTG GCTCCACCAG CCCGGATTCA CCAGGGGGAG CCAAAGGGGA GCCGCCTCCA 
CCAGTAGGAC CTGGTGACAG TGTAGGGCTG CTGGGAACTG GAAGGGAAGG GACGGCGGGA 
CGGGGAGGAA GCCGGGCTGG GTCCCAGCAC TTGACCCCAG CCGCACTGCT GTACAGGGCT 
GCTGTCACCC GAAGTCAGAA ACGCGGCATC TCATCAGAGG AAGAGGAAGG AGAGGTGGAC 
AGTGAAGTGG AACTGGCCTC AAGCCAGAGG TGGCCTCAGG GCCTGAAAAT GCGCCAGTCA 
CTATCTACCT TCAGCTCAGA GAATCCATCG GATGGGGAGG AGGGCACAGC TAGTGAGCCT 
TCCCCCAGTG GCACACCTGA AGTTGGCAGT ACCAACACAG ATGAGCGGCC GGATGAACGG 
TCTGATGACA TGTGCTCGCA GGGCTCAGAA ATCCCACTGG ACCCACCAGC TTCTGAGGTG 
GTTACTGGCC CAGAACCCAG CTCCTTGCCC GTCCCACACC ACGACCTACT CAGAGGGGAG 
CAGGGCCCTC CCAACCCTGA GGACTCAGAC TGTGACAGCA CTGAACTGGA CAACGCCAAC 
AGCGGTGAAG CCTTGCGGCC CCCAGCCTCC CTCCCTCCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005206198.1
CDS	752..3433
Translation	MACLHETRTPSPSFGGFVSTLSEASMRKLDPDTSDCTPEKDLTPTQCVLRDVVPLGGQGGGGPSPSPGGEPPPEPFANSVLQLHEQDAGGPGGATGSPESRASRVRADEVRLQCQSGSGFLEGLFGCLRPVWTMIGKAYSTEHKQQQEDLWEVPFEEILDLQWVGSGAQGAVFLGRFHGEEVAVKKVRDLKETDIKHLRKLKHPNIITFKGVCTQAPCYCILMEFCAQGQLYEVLRAGRPVTPSLLVDWSMGIAGGMNYLHLHKIIHRDLKSPNMLITYDDVVKISDFGTSKELSDKSTKMSFAGTVAWMAPEVIRNEPVSEKVDIWSFGVVLWELLTGEIPYKDVDSSAIIWGVGSNSLHLPVPSSCPDGFKILLRQCWNSKPRNRPSFRQILLHLDIASADVLSTPQETYFKSQAEWREEVKLHFEKIKSEGTCLHRLEEELVMRRREELRHALDIREHYERKLERANNLYMELNALMLQLELKERELLRREQALERRCPGLLKSHPSRGLLHGNTMEKLIKKRNVPQKLSPHSKRPDILKTESLLPKLDAALSGVGLPGCAKGPPSPGRSRRGKTRHRKASAKGSCGDLPGLRAAVPPHEPGGPGSPVGLGGGPSAWEACPPALRGLHHDLLLRKMSSSSPDLLSAALGARGRGATGGAGDPGSPPPARGDTPPSEGSAPGSTSPDSPGGAKGEPPPPVGPGDSVGLLGTGREGTAGRGGSRAGSQHLTPAALLYRAAVTRSQKRGISSEEEEGEVDSEVELASSQRWPQGLKMRQSLSTFSSENPSDGEEGTASEPSPSGTPEVGSTNTDERPDERSDDMCSQGSEIPLDPPASEVVTGPEPSSLPVPHHDLLRGEQGPPNPEDSDCDSTELDNANSGEALRPPASLPP

Target ORF information:

RefSeq Version	XM_005206141.3
Organism	Bos taurus(cattle)
Definition	Bos taurus mitogen-activated protein kinase kinase kinase 12 (MAP3K12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005206141.3

ORF Insert Sequence:

ATGGCCTGCC TCCATGAGAC CCGCACACCC TCCCCTTCCT TTGGGGGTTT CGTGTCCACC 
CTAAGCGAGG CATCCATGCG CAAGCTGGAC CCAGACACTT CCGACTGCAC CCCCGAGAAG 
GACCTGACGC CCACCCAGTG TGTACTCCGA GACGTAGTGC CACTCGGGGG TCAGGGCGGG 
GGCGGGCCCA GCCCCTCCCC AGGTGGAGAG CCGCCCCCTG AGCCTTTTGC CAACAGTGTC 
CTGCAGCTCC ACGAGCAGGA TGCAGGGGGC CCAGGGGGAG CCACTGGGTC CCCTGAGAGT 
CGGGCGTCCA GGGTCCGAGC GGATGAGGTA CGGCTCCAGT GCCAGAGCGG CAGCGGCTTC 
CTGGAGGGCC TCTTCGGCTG CCTGCGCCCT GTGTGGACCA TGATCGGCAA AGCCTACTCC 
ACGGAGCACA AGCAGCAGCA GGAAGACCTT TGGGAGGTCC CCTTTGAGGA AATCCTGGAC 
CTGCAGTGGG TGGGCTCAGG GGCCCAGGGC GCCGTCTTCC TGGGGCGCTT CCATGGGGAG 
GAGGTGGCTG TGAAGAAGGT GCGGGACCTG AAGGAAACTG ACATCAAGCA CCTGCGAAAG 
CTGAAGCACC CAAACATCAT CACCTTCAAG GGTGTGTGCA CCCAGGCTCC CTGCTACTGC 
ATCCTCATGG AGTTCTGCGC CCAGGGCCAG CTGTACGAGG TGCTGCGGGC TGGCCGCCCC 
GTCACGCCCT CCTTGCTGGT TGACTGGTCC ATGGGCATCG CCGGCGGCAT GAACTACCTG 
CACCTGCACA AGATTATCCA TCGGGACCTC AAGTCCCCCA ACATGCTAAT CACCTACGAC 
GATGTGGTGA AGATCTCCGA TTTTGGCACT TCCAAGGAGC TGAGTGACAA GAGCACCAAG 
ATGTCCTTTG CAGGGACAGT AGCCTGGATG GCCCCTGAAG TGATCCGCAA CGAACCTGTG 
TCTGAGAAGG TTGACATCTG GTCCTTTGGC GTGGTGCTGT GGGAGCTGCT GACTGGTGAG 
ATCCCCTACA AAGACGTAGA TTCCTCAGCC ATCATCTGGG GTGTGGGAAG CAACAGTCTC 
CATCTACCTG TGCCCTCCAG CTGCCCAGAC GGCTTCAAAA TCCTGCTTCG CCAGTGCTGG 
AACAGCAAAC CACGAAACCG CCCATCATTC CGACAGATCC TGCTGCATCT GGACATTGCC 
TCAGCCGACG TACTCTCCAC ACCCCAGGAG ACTTACTTTA AGTCCCAGGC AGAGTGGCGT 
GAAGAGGTAA AGCTGCATTT TGAAAAAATT AAGTCAGAAG GGACCTGTCT GCACCGCCTA 
GAAGAGGAGC TGGTGATGCG GAGGAGGGAG GAGCTCAGAC ATGCCCTGGA CATTAGGGAA 
CACTATGAGC GGAAACTGGA GAGAGCCAAC AACCTGTACA TGGAACTTAA TGCCCTTATG 
TTGCAGCTGG AGCTGAAGGA ACGGGAGCTA CTCAGGAGGG AGCAAGCTTT AGAGCGGCGG 
TGTCCAGGTC TGCTGAAGTC ACACCCTTCC CGGGGCCTGC TGCACGGGAA CACGATGGAG 
AAACTGATCA AGAAGAGGAA TGTCCCACAG AAACTGTCGC CCCACAGCAA AAGGCCAGAT 
ATCCTCAAGA CTGAGTCTTT GCTACCCAAA CTAGATGCAG CCCTGAGTGG GGTGGGGCTT 
CCTGGGTGTG CTAAGGGCCC CCCCTCACCA GGACGGAGTC GCCGTGGCAA GACTCGTCAC 
CGCAAGGCCA GCGCCAAGGG CAGCTGTGGG GACCTGCCTG GGCTTCGTGC AGCTGTGCCA 
CCCCATGAAC CTGGGGGACC AGGAAGCCCC GTGGGGCTAG GCGGGGGACC CTCAGCCTGG 
GAAGCCTGCC CTCCAGCCCT CCGTGGGCTC CACCATGATC TCCTGCTCCG AAAGATGTCT 
TCATCATCGC CAGATCTGCT GTCAGCAGCA TTGGGAGCCC GGGGCCGGGG GGCTACAGGG 
GGAGCTGGGG ATCCTGGCTC ACCACCTCCA GCCCGGGGAG ACACGCCCCC AAGCGAGGGC 
TCAGCACCTG GCTCCACCAG CCCGGATTCA CCAGGGGGAG CCAAAGGGGA GCCGCCTCCA 
CCAGTAGGAC CTGGTGACAG TGTAGGGCTG CTGGGAACTG GAAGGGAAGG GACGGCGGGA 
CGGGGAGGAA GCCGGGCTGG GTCCCAGCAC TTGACCCCAG CCGCACTGCT GTACAGGGCT 
GCTGTCACCC GAAGTCAGAA ACGCGGCATC TCATCAGAGG AAGAGGAAGG AGAGGTGGAC 
AGTGAAGTGG AACTGGCCTC AAGCCAGAGG TGGCCTCAGG GCCTGAAAAT GCGCCAGTCA 
CTATCTACCT TCAGCTCAGA GAATCCATCG GATGGGGAGG AGGGCACAGC TAGTGAGCCT 
TCCCCCAGTG GCACACCTGA AGTTGGCAGT ACCAACACAG ATGAGCGGCC GGATGAACGG 
TCTGATGACA TGTGCTCGCA GGGCTCAGAA ATCCCACTGG ACCCACCAGC TTCTGAGGTG 
GTTACTGGCC CAGAACCCAG CTCCTTGCCC GTCCCACACC ACGACCTACT CAGAGGGGAG 
CAGGGCCCTC CCAACCCTGA GGACTCAGAC TGTGACAGCA CTGAACTGGA CAACGCCAAC 
AGCGGTGAAG CCTTGCGGCC CCCAGCCTCC CTCCCTCCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K12 ( XM_024991461.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024847229.1 Bos taurus mitogen-activated protein kinase kinase kinase 12 (MAP3K12), transcript variant X1, mRNA.

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CloneID	OBa19101
Clone ID Related Accession (Same CDS sequence)	XM_005206141.3 , XM_024991461.1 , XM_005206141.4
Accession Version	XM_024991461.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2682bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	mitogen-activated protein kinase kinase kinase 12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTGCC TCCATGAGAC CCGCACACCC TCCCCTTCCT TTGGGGGTTT CGTGTCCACC 
CTAAGCGAGG CATCCATGCG CAAGCTGGAC CCAGACACTT CCGACTGCAC CCCCGAGAAG 
GACCTGACGC CCACCCAGTG TGTACTCCGA GACGTAGTGC CACTCGGGGG TCAGGGCGGG 
GGCGGGCCCA GCCCCTCCCC AGGTGGAGAG CCGCCCCCTG AGCCTTTTGC CAACAGTGTC 
CTGCAGCTCC ACGAGCAGGA TGCAGGGGGC CCAGGGGGAG CCACTGGGTC CCCTGAGAGT 
CGGGCGTCCA GGGTCCGAGC GGATGAGGTA CGGCTCCAGT GCCAGAGCGG CAGCGGCTTC 
CTGGAGGGCC TCTTCGGCTG CCTGCGCCCT GTGTGGACCA TGATCGGCAA AGCCTACTCC 
ACGGAGCACA AGCAGCAGCA GGAAGACCTT TGGGAGGTCC CCTTTGAGGA AATCCTGGAC 
CTGCAGTGGG TGGGCTCAGG GGCCCAGGGC GCCGTCTTCC TGGGGCGCTT CCATGGGGAG 
GAGGTGGCTG TGAAGAAGGT GCGGGACCTG AAGGAAACTG ACATCAAGCA CCTGCGAAAG 
CTGAAGCACC CAAACATCAT CACCTTCAAG GGTGTGTGCA CCCAGGCTCC CTGCTACTGC 
ATCCTCATGG AGTTCTGCGC CCAGGGCCAG CTGTACGAGG TGCTGCGGGC TGGCCGCCCC 
GTCACGCCCT CCTTGCTGGT TGACTGGTCC ATGGGCATCG CCGGCGGCAT GAACTACCTG 
CACCTGCACA AGATTATCCA TCGGGACCTC AAGTCCCCCA ACATGCTAAT CACCTACGAC 
GATGTGGTGA AGATCTCCGA TTTTGGCACT TCCAAGGAGC TGAGTGACAA GAGCACCAAG 
ATGTCCTTTG CAGGGACAGT AGCCTGGATG GCCCCTGAAG TGATCCGCAA CGAACCTGTG 
TCTGAGAAGG TTGACATCTG GTCCTTTGGC GTGGTGCTGT GGGAGCTGCT GACTGGTGAG 
ATCCCCTACA AAGACGTAGA TTCCTCAGCC ATCATCTGGG GTGTGGGAAG CAACAGTCTC 
CATCTACCTG TGCCCTCCAG CTGCCCAGAC GGCTTCAAAA TCCTGCTTCG CCAGTGCTGG 
AACAGCAAAC CACGAAACCG CCCATCATTC CGACAGATCC TGCTGCATCT GGACATTGCC 
TCAGCCGACG TACTCTCCAC ACCCCAGGAG ACTTACTTTA AGTCCCAGGC AGAGTGGCGT 
GAAGAGGTAA AGCTGCATTT TGAAAAAATT AAGTCAGAAG GGACCTGTCT GCACCGCCTA 
GAAGAGGAGC TGGTGATGCG GAGGAGGGAG GAGCTCAGAC ATGCCCTGGA CATTAGGGAA 
CACTATGAGC GGAAACTGGA GAGAGCCAAC AACCTGTACA TGGAACTTAA TGCCCTTATG 
TTGCAGCTGG AGCTGAAGGA ACGGGAGCTA CTCAGGAGGG AGCAAGCTTT AGAGCGGCGG 
TGTCCAGGTC TGCTGAAGTC ACACCCTTCC CGGGGCCTGC TGCACGGGAA CACGATGGAG 
AAACTGATCA AGAAGAGGAA TGTCCCACAG AAACTGTCGC CCCACAGCAA AAGGCCAGAT 
ATCCTCAAGA CTGAGTCTTT GCTACCCAAA CTAGATGCAG CCCTGAGTGG GGTGGGGCTT 
CCTGGGTGTG CTAAGGGCCC CCCCTCACCA GGACGGAGTC GCCGTGGCAA GACTCGTCAC 
CGCAAGGCCA GCGCCAAGGG CAGCTGTGGG GACCTGCCTG GGCTTCGTGC AGCTGTGCCA 
CCCCATGAAC CTGGGGGACC AGGAAGCCCC GTGGGGCTAG GCGGGGGACC CTCAGCCTGG 
GAAGCCTGCC CTCCAGCCCT CCGTGGGCTC CACCATGATC TCCTGCTCCG AAAGATGTCT 
TCATCATCGC CAGATCTGCT GTCAGCAGCA TTGGGAGCCC GGGGCCGGGG GGCTACAGGG 
GGAGCTGGGG ATCCTGGCTC ACCACCTCCA GCCCGGGGAG ACACGCCCCC AAGCGAGGGC 
TCAGCACCTG GCTCCACCAG CCCGGATTCA CCAGGGGGAG CCAAAGGGGA GCCGCCTCCA 
CCAGTAGGAC CTGGTGACAG TGTAGGGCTG CTGGGAACTG GAAGGGAAGG GACGGCGGGA 
CGGGGAGGAA GCCGGGCTGG GTCCCAGCAC TTGACCCCAG CCGCACTGCT GTACAGGGCT 
GCTGTCACCC GAAGTCAGAA ACGCGGCATC TCATCAGAGG AAGAGGAAGG AGAGGTGGAC 
AGTGAAGTGG AACTGGCCTC AAGCCAGAGG TGGCCTCAGG GCCTGAAAAT GCGCCAGTCA 
CTATCTACCT TCAGCTCAGA GAATCCATCG GATGGGGAGG AGGGCACAGC TAGTGAGCCT 
TCCCCCAGTG GCACACCTGA AGTTGGCAGT ACCAACACAG ATGAGCGGCC GGATGAACGG 
TCTGATGACA TGTGCTCGCA GGGCTCAGAA ATCCCACTGG ACCCACCAGC TTCTGAGGTG 
GTTACTGGCC CAGAACCCAG CTCCTTGCCC GTCCCACACC ACGACCTACT CAGAGGGGAG 
CAGGGCCCTC CCAACCCTGA GGACTCAGAC TGTGACAGCA CTGAACTGGA CAACGCCAAC 
AGCGGTGAAG CCTTGCGGCC CCCAGCCTCC CTCCCTCCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024847229.1
CDS	232..2913
Translation	MACLHETRTPSPSFGGFVSTLSEASMRKLDPDTSDCTPEKDLTPTQCVLRDVVPLGGQGGGGPSPSPGGEPPPEPFANSVLQLHEQDAGGPGGATGSPESRASRVRADEVRLQCQSGSGFLEGLFGCLRPVWTMIGKAYSTEHKQQQEDLWEVPFEEILDLQWVGSGAQGAVFLGRFHGEEVAVKKVRDLKETDIKHLRKLKHPNIITFKGVCTQAPCYCILMEFCAQGQLYEVLRAGRPVTPSLLVDWSMGIAGGMNYLHLHKIIHRDLKSPNMLITYDDVVKISDFGTSKELSDKSTKMSFAGTVAWMAPEVIRNEPVSEKVDIWSFGVVLWELLTGEIPYKDVDSSAIIWGVGSNSLHLPVPSSCPDGFKILLRQCWNSKPRNRPSFRQILLHLDIASADVLSTPQETYFKSQAEWREEVKLHFEKIKSEGTCLHRLEEELVMRRREELRHALDIREHYERKLERANNLYMELNALMLQLELKERELLRREQALERRCPGLLKSHPSRGLLHGNTMEKLIKKRNVPQKLSPHSKRPDILKTESLLPKLDAALSGVGLPGCAKGPPSPGRSRRGKTRHRKASAKGSCGDLPGLRAAVPPHEPGGPGSPVGLGGGPSAWEACPPALRGLHHDLLLRKMSSSSPDLLSAALGARGRGATGGAGDPGSPPPARGDTPPSEGSAPGSTSPDSPGGAKGEPPPPVGPGDSVGLLGTGREGTAGRGGSRAGSQHLTPAALLYRAAVTRSQKRGISSEEEEGEVDSEVELASSQRWPQGLKMRQSLSTFSSENPSDGEEGTASEPSPSGTPEVGSTNTDERPDERSDDMCSQGSEIPLDPPASEVVTGPEPSSLPVPHHDLLRGEQGPPNPEDSDCDSTELDNANSGEALRPPASLPP

Target ORF information:

RefSeq Version	XM_024991461.1
Organism	Bos taurus(cattle)
Definition	Bos taurus mitogen-activated protein kinase kinase kinase 12 (MAP3K12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024991461.1

ORF Insert Sequence:

ATGGCCTGCC TCCATGAGAC CCGCACACCC TCCCCTTCCT TTGGGGGTTT CGTGTCCACC 
CTAAGCGAGG CATCCATGCG CAAGCTGGAC CCAGACACTT CCGACTGCAC CCCCGAGAAG 
GACCTGACGC CCACCCAGTG TGTACTCCGA GACGTAGTGC CACTCGGGGG TCAGGGCGGG 
GGCGGGCCCA GCCCCTCCCC AGGTGGAGAG CCGCCCCCTG AGCCTTTTGC CAACAGTGTC 
CTGCAGCTCC ACGAGCAGGA TGCAGGGGGC CCAGGGGGAG CCACTGGGTC CCCTGAGAGT 
CGGGCGTCCA GGGTCCGAGC GGATGAGGTA CGGCTCCAGT GCCAGAGCGG CAGCGGCTTC 
CTGGAGGGCC TCTTCGGCTG CCTGCGCCCT GTGTGGACCA TGATCGGCAA AGCCTACTCC 
ACGGAGCACA AGCAGCAGCA GGAAGACCTT TGGGAGGTCC CCTTTGAGGA AATCCTGGAC 
CTGCAGTGGG TGGGCTCAGG GGCCCAGGGC GCCGTCTTCC TGGGGCGCTT CCATGGGGAG 
GAGGTGGCTG TGAAGAAGGT GCGGGACCTG AAGGAAACTG ACATCAAGCA CCTGCGAAAG 
CTGAAGCACC CAAACATCAT CACCTTCAAG GGTGTGTGCA CCCAGGCTCC CTGCTACTGC 
ATCCTCATGG AGTTCTGCGC CCAGGGCCAG CTGTACGAGG TGCTGCGGGC TGGCCGCCCC 
GTCACGCCCT CCTTGCTGGT TGACTGGTCC ATGGGCATCG CCGGCGGCAT GAACTACCTG 
CACCTGCACA AGATTATCCA TCGGGACCTC AAGTCCCCCA ACATGCTAAT CACCTACGAC 
GATGTGGTGA AGATCTCCGA TTTTGGCACT TCCAAGGAGC TGAGTGACAA GAGCACCAAG 
ATGTCCTTTG CAGGGACAGT AGCCTGGATG GCCCCTGAAG TGATCCGCAA CGAACCTGTG 
TCTGAGAAGG TTGACATCTG GTCCTTTGGC GTGGTGCTGT GGGAGCTGCT GACTGGTGAG 
ATCCCCTACA AAGACGTAGA TTCCTCAGCC ATCATCTGGG GTGTGGGAAG CAACAGTCTC 
CATCTACCTG TGCCCTCCAG CTGCCCAGAC GGCTTCAAAA TCCTGCTTCG CCAGTGCTGG 
AACAGCAAAC CACGAAACCG CCCATCATTC CGACAGATCC TGCTGCATCT GGACATTGCC 
TCAGCCGACG TACTCTCCAC ACCCCAGGAG ACTTACTTTA AGTCCCAGGC AGAGTGGCGT 
GAAGAGGTAA AGCTGCATTT TGAAAAAATT AAGTCAGAAG GGACCTGTCT GCACCGCCTA 
GAAGAGGAGC TGGTGATGCG GAGGAGGGAG GAGCTCAGAC ATGCCCTGGA CATTAGGGAA 
CACTATGAGC GGAAACTGGA GAGAGCCAAC AACCTGTACA TGGAACTTAA TGCCCTTATG 
TTGCAGCTGG AGCTGAAGGA ACGGGAGCTA CTCAGGAGGG AGCAAGCTTT AGAGCGGCGG 
TGTCCAGGTC TGCTGAAGTC ACACCCTTCC CGGGGCCTGC TGCACGGGAA CACGATGGAG 
AAACTGATCA AGAAGAGGAA TGTCCCACAG AAACTGTCGC CCCACAGCAA AAGGCCAGAT 
ATCCTCAAGA CTGAGTCTTT GCTACCCAAA CTAGATGCAG CCCTGAGTGG GGTGGGGCTT 
CCTGGGTGTG CTAAGGGCCC CCCCTCACCA GGACGGAGTC GCCGTGGCAA GACTCGTCAC 
CGCAAGGCCA GCGCCAAGGG CAGCTGTGGG GACCTGCCTG GGCTTCGTGC AGCTGTGCCA 
CCCCATGAAC CTGGGGGACC AGGAAGCCCC GTGGGGCTAG GCGGGGGACC CTCAGCCTGG 
GAAGCCTGCC CTCCAGCCCT CCGTGGGCTC CACCATGATC TCCTGCTCCG AAAGATGTCT 
TCATCATCGC CAGATCTGCT GTCAGCAGCA TTGGGAGCCC GGGGCCGGGG GGCTACAGGG 
GGAGCTGGGG ATCCTGGCTC ACCACCTCCA GCCCGGGGAG ACACGCCCCC AAGCGAGGGC 
TCAGCACCTG GCTCCACCAG CCCGGATTCA CCAGGGGGAG CCAAAGGGGA GCCGCCTCCA 
CCAGTAGGAC CTGGTGACAG TGTAGGGCTG CTGGGAACTG GAAGGGAAGG GACGGCGGGA 
CGGGGAGGAA GCCGGGCTGG GTCCCAGCAC TTGACCCCAG CCGCACTGCT GTACAGGGCT 
GCTGTCACCC GAAGTCAGAA ACGCGGCATC TCATCAGAGG AAGAGGAAGG AGAGGTGGAC 
AGTGAAGTGG AACTGGCCTC AAGCCAGAGG TGGCCTCAGG GCCTGAAAAT GCGCCAGTCA 
CTATCTACCT TCAGCTCAGA GAATCCATCG GATGGGGAGG AGGGCACAGC TAGTGAGCCT 
TCCCCCAGTG GCACACCTGA AGTTGGCAGT ACCAACACAG ATGAGCGGCC GGATGAACGG 
TCTGATGACA TGTGCTCGCA GGGCTCAGAA ATCCCACTGG ACCCACCAGC TTCTGAGGTG 
GTTACTGGCC CAGAACCCAG CTCCTTGCCC GTCCCACACC ACGACCTACT CAGAGGGGAG 
CAGGGCCCTC CCAACCCTGA GGACTCAGAC TGTGACAGCA CTGAACTGGA CAACGCCAAC 
AGCGGTGAAG CCTTGCGGCC CCCAGCCTCC CTCCCTCCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K12 ( XM_005206141.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005206198.1 Bos taurus mitogen-activated protein kinase kinase kinase 12 (MAP3K12), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa19101
Clone ID Related Accession (Same CDS sequence)	XM_005206141.3 , XM_024991461.1 , XM_005206141.4
Accession Version	XM_005206141.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2682bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	mitogen-activated protein kinase kinase kinase 12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005206141.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTGCC TCCATGAGAC CCGCACACCC TCCCCTTCCT TTGGGGGTTT CGTGTCCACC 
CTAAGCGAGG CATCCATGCG CAAGCTGGAC CCAGACACTT CCGACTGCAC CCCCGAGAAG 
GACCTGACGC CCACCCAGTG TGTACTCCGA GACGTAGTGC CACTCGGGGG TCAGGGCGGG 
GGCGGGCCCA GCCCCTCCCC AGGTGGAGAG CCGCCCCCTG AGCCTTTTGC CAACAGTGTC 
CTGCAGCTCC ACGAGCAGGA TGCAGGGGGC CCAGGGGGAG CCACTGGGTC CCCTGAGAGT 
CGGGCGTCCA GGGTCCGAGC GGATGAGGTA CGGCTCCAGT GCCAGAGCGG CAGCGGCTTC 
CTGGAGGGCC TCTTCGGCTG CCTGCGCCCT GTGTGGACCA TGATCGGCAA AGCCTACTCC 
ACGGAGCACA AGCAGCAGCA GGAAGACCTT TGGGAGGTCC CCTTTGAGGA AATCCTGGAC 
CTGCAGTGGG TGGGCTCAGG GGCCCAGGGC GCCGTCTTCC TGGGGCGCTT CCATGGGGAG 
GAGGTGGCTG TGAAGAAGGT GCGGGACCTG AAGGAAACTG ACATCAAGCA CCTGCGAAAG 
CTGAAGCACC CAAACATCAT CACCTTCAAG GGTGTGTGCA CCCAGGCTCC CTGCTACTGC 
ATCCTCATGG AGTTCTGCGC CCAGGGCCAG CTGTACGAGG TGCTGCGGGC TGGCCGCCCC 
GTCACGCCCT CCTTGCTGGT TGACTGGTCC ATGGGCATCG CCGGCGGCAT GAACTACCTG 
CACCTGCACA AGATTATCCA TCGGGACCTC AAGTCCCCCA ACATGCTAAT CACCTACGAC 
GATGTGGTGA AGATCTCCGA TTTTGGCACT TCCAAGGAGC TGAGTGACAA GAGCACCAAG 
ATGTCCTTTG CAGGGACAGT AGCCTGGATG GCCCCTGAAG TGATCCGCAA CGAACCTGTG 
TCTGAGAAGG TTGACATCTG GTCCTTTGGC GTGGTGCTGT GGGAGCTGCT GACTGGTGAG 
ATCCCCTACA AAGACGTAGA TTCCTCAGCC ATCATCTGGG GTGTGGGAAG CAACAGTCTC 
CATCTACCTG TGCCCTCCAG CTGCCCAGAC GGCTTCAAAA TCCTGCTTCG CCAGTGCTGG 
AACAGCAAAC CACGAAACCG CCCATCATTC CGACAGATCC TGCTGCATCT GGACATTGCC 
TCAGCCGACG TACTCTCCAC ACCCCAGGAG ACTTACTTTA AGTCCCAGGC AGAGTGGCGT 
GAAGAGGTAA AGCTGCATTT TGAAAAAATT AAGTCAGAAG GGACCTGTCT GCACCGCCTA 
GAAGAGGAGC TGGTGATGCG GAGGAGGGAG GAGCTCAGAC ATGCCCTGGA CATTAGGGAA 
CACTATGAGC GGAAACTGGA GAGAGCCAAC AACCTGTACA TGGAACTTAA TGCCCTTATG 
TTGCAGCTGG AGCTGAAGGA ACGGGAGCTA CTCAGGAGGG AGCAAGCTTT AGAGCGGCGG 
TGTCCAGGTC TGCTGAAGTC ACACCCTTCC CGGGGCCTGC TGCACGGGAA CACGATGGAG 
AAACTGATCA AGAAGAGGAA TGTCCCACAG AAACTGTCGC CCCACAGCAA AAGGCCAGAT 
ATCCTCAAGA CTGAGTCTTT GCTACCCAAA CTAGATGCAG CCCTGAGTGG GGTGGGGCTT 
CCTGGGTGTG CTAAGGGCCC CCCCTCACCA GGACGGAGTC GCCGTGGCAA GACTCGTCAC 
CGCAAGGCCA GCGCCAAGGG CAGCTGTGGG GACCTGCCTG GGCTTCGTGC AGCTGTGCCA 
CCCCATGAAC CTGGGGGACC AGGAAGCCCC GTGGGGCTAG GCGGGGGACC CTCAGCCTGG 
GAAGCCTGCC CTCCAGCCCT CCGTGGGCTC CACCATGATC TCCTGCTCCG AAAGATGTCT 
TCATCATCGC CAGATCTGCT GTCAGCAGCA TTGGGAGCCC GGGGCCGGGG GGCTACAGGG 
GGAGCTGGGG ATCCTGGCTC ACCACCTCCA GCCCGGGGAG ACACGCCCCC AAGCGAGGGC 
TCAGCACCTG GCTCCACCAG CCCGGATTCA CCAGGGGGAG CCAAAGGGGA GCCGCCTCCA 
CCAGTAGGAC CTGGTGACAG TGTAGGGCTG CTGGGAACTG GAAGGGAAGG GACGGCGGGA 
CGGGGAGGAA GCCGGGCTGG GTCCCAGCAC TTGACCCCAG CCGCACTGCT GTACAGGGCT 
GCTGTCACCC GAAGTCAGAA ACGCGGCATC TCATCAGAGG AAGAGGAAGG AGAGGTGGAC 
AGTGAAGTGG AACTGGCCTC AAGCCAGAGG TGGCCTCAGG GCCTGAAAAT GCGCCAGTCA 
CTATCTACCT TCAGCTCAGA GAATCCATCG GATGGGGAGG AGGGCACAGC TAGTGAGCCT 
TCCCCCAGTG GCACACCTGA AGTTGGCAGT ACCAACACAG ATGAGCGGCC GGATGAACGG 
TCTGATGACA TGTGCTCGCA GGGCTCAGAA ATCCCACTGG ACCCACCAGC TTCTGAGGTG 
GTTACTGGCC CAGAACCCAG CTCCTTGCCC GTCCCACACC ACGACCTACT CAGAGGGGAG 
CAGGGCCCTC CCAACCCTGA GGACTCAGAC TGTGACAGCA CTGAACTGGA CAACGCCAAC 
AGCGGTGAAG CCTTGCGGCC CCCAGCCTCC CTCCCTCCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005206198.1
CDS	716..3397
Translation	MACLHETRTPSPSFGGFVSTLSEASMRKLDPDTSDCTPEKDLTPTQCVLRDVVPLGGQGGGGPSPSPGGEPPPEPFANSVLQLHEQDAGGPGGATGSPESRASRVRADEVRLQCQSGSGFLEGLFGCLRPVWTMIGKAYSTEHKQQQEDLWEVPFEEILDLQWVGSGAQGAVFLGRFHGEEVAVKKVRDLKETDIKHLRKLKHPNIITFKGVCTQAPCYCILMEFCAQGQLYEVLRAGRPVTPSLLVDWSMGIAGGMNYLHLHKIIHRDLKSPNMLITYDDVVKISDFGTSKELSDKSTKMSFAGTVAWMAPEVIRNEPVSEKVDIWSFGVVLWELLTGEIPYKDVDSSAIIWGVGSNSLHLPVPSSCPDGFKILLRQCWNSKPRNRPSFRQILLHLDIASADVLSTPQETYFKSQAEWREEVKLHFEKIKSEGTCLHRLEEELVMRRREELRHALDIREHYERKLERANNLYMELNALMLQLELKERELLRREQALERRCPGLLKSHPSRGLLHGNTMEKLIKKRNVPQKLSPHSKRPDILKTESLLPKLDAALSGVGLPGCAKGPPSPGRSRRGKTRHRKASAKGSCGDLPGLRAAVPPHEPGGPGSPVGLGGGPSAWEACPPALRGLHHDLLLRKMSSSSPDLLSAALGARGRGATGGAGDPGSPPPARGDTPPSEGSAPGSTSPDSPGGAKGEPPPPVGPGDSVGLLGTGREGTAGRGGSRAGSQHLTPAALLYRAAVTRSQKRGISSEEEEGEVDSEVELASSQRWPQGLKMRQSLSTFSSENPSDGEEGTASEPSPSGTPEVGSTNTDERPDERSDDMCSQGSEIPLDPPASEVVTGPEPSSLPVPHHDLLRGEQGPPNPEDSDCDSTELDNANSGEALRPPASLPP

Target ORF information:

RefSeq Version	XM_005206141.4
Organism	Bos taurus(cattle)
Definition	Bos taurus mitogen-activated protein kinase kinase kinase 12 (MAP3K12), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005206141.4

ORF Insert Sequence:

ATGGCCTGCC TCCATGAGAC CCGCACACCC TCCCCTTCCT TTGGGGGTTT CGTGTCCACC 
CTAAGCGAGG CATCCATGCG CAAGCTGGAC CCAGACACTT CCGACTGCAC CCCCGAGAAG 
GACCTGACGC CCACCCAGTG TGTACTCCGA GACGTAGTGC CACTCGGGGG TCAGGGCGGG 
GGCGGGCCCA GCCCCTCCCC AGGTGGAGAG CCGCCCCCTG AGCCTTTTGC CAACAGTGTC 
CTGCAGCTCC ACGAGCAGGA TGCAGGGGGC CCAGGGGGAG CCACTGGGTC CCCTGAGAGT 
CGGGCGTCCA GGGTCCGAGC GGATGAGGTA CGGCTCCAGT GCCAGAGCGG CAGCGGCTTC 
CTGGAGGGCC TCTTCGGCTG CCTGCGCCCT GTGTGGACCA TGATCGGCAA AGCCTACTCC 
ACGGAGCACA AGCAGCAGCA GGAAGACCTT TGGGAGGTCC CCTTTGAGGA AATCCTGGAC 
CTGCAGTGGG TGGGCTCAGG GGCCCAGGGC GCCGTCTTCC TGGGGCGCTT CCATGGGGAG 
GAGGTGGCTG TGAAGAAGGT GCGGGACCTG AAGGAAACTG ACATCAAGCA CCTGCGAAAG 
CTGAAGCACC CAAACATCAT CACCTTCAAG GGTGTGTGCA CCCAGGCTCC CTGCTACTGC 
ATCCTCATGG AGTTCTGCGC CCAGGGCCAG CTGTACGAGG TGCTGCGGGC TGGCCGCCCC 
GTCACGCCCT CCTTGCTGGT TGACTGGTCC ATGGGCATCG CCGGCGGCAT GAACTACCTG 
CACCTGCACA AGATTATCCA TCGGGACCTC AAGTCCCCCA ACATGCTAAT CACCTACGAC 
GATGTGGTGA AGATCTCCGA TTTTGGCACT TCCAAGGAGC TGAGTGACAA GAGCACCAAG 
ATGTCCTTTG CAGGGACAGT AGCCTGGATG GCCCCTGAAG TGATCCGCAA CGAACCTGTG 
TCTGAGAAGG TTGACATCTG GTCCTTTGGC GTGGTGCTGT GGGAGCTGCT GACTGGTGAG 
ATCCCCTACA AAGACGTAGA TTCCTCAGCC ATCATCTGGG GTGTGGGAAG CAACAGTCTC 
CATCTACCTG TGCCCTCCAG CTGCCCAGAC GGCTTCAAAA TCCTGCTTCG CCAGTGCTGG 
AACAGCAAAC CACGAAACCG CCCATCATTC CGACAGATCC TGCTGCATCT GGACATTGCC 
TCAGCCGACG TACTCTCCAC ACCCCAGGAG ACTTACTTTA AGTCCCAGGC AGAGTGGCGT 
GAAGAGGTAA AGCTGCATTT TGAAAAAATT AAGTCAGAAG GGACCTGTCT GCACCGCCTA 
GAAGAGGAGC TGGTGATGCG GAGGAGGGAG GAGCTCAGAC ATGCCCTGGA CATTAGGGAA 
CACTATGAGC GGAAACTGGA GAGAGCCAAC AACCTGTACA TGGAACTTAA TGCCCTTATG 
TTGCAGCTGG AGCTGAAGGA ACGGGAGCTA CTCAGGAGGG AGCAAGCTTT AGAGCGGCGG 
TGTCCAGGTC TGCTGAAGTC ACACCCTTCC CGGGGCCTGC TGCACGGGAA CACGATGGAG 
AAACTGATCA AGAAGAGGAA TGTCCCACAG AAACTGTCGC CCCACAGCAA AAGGCCAGAT 
ATCCTCAAGA CTGAGTCTTT GCTACCCAAA CTAGATGCAG CCCTGAGTGG GGTGGGGCTT 
CCTGGGTGTG CTAAGGGCCC CCCCTCACCA GGACGGAGTC GCCGTGGCAA GACTCGTCAC 
CGCAAGGCCA GCGCCAAGGG CAGCTGTGGG GACCTGCCTG GGCTTCGTGC AGCTGTGCCA 
CCCCATGAAC CTGGGGGACC AGGAAGCCCC GTGGGGCTAG GCGGGGGACC CTCAGCCTGG 
GAAGCCTGCC CTCCAGCCCT CCGTGGGCTC CACCATGATC TCCTGCTCCG AAAGATGTCT 
TCATCATCGC CAGATCTGCT GTCAGCAGCA TTGGGAGCCC GGGGCCGGGG GGCTACAGGG 
GGAGCTGGGG ATCCTGGCTC ACCACCTCCA GCCCGGGGAG ACACGCCCCC AAGCGAGGGC 
TCAGCACCTG GCTCCACCAG CCCGGATTCA CCAGGGGGAG CCAAAGGGGA GCCGCCTCCA 
CCAGTAGGAC CTGGTGACAG TGTAGGGCTG CTGGGAACTG GAAGGGAAGG GACGGCGGGA 
CGGGGAGGAA GCCGGGCTGG GTCCCAGCAC TTGACCCCAG CCGCACTGCT GTACAGGGCT 
GCTGTCACCC GAAGTCAGAA ACGCGGCATC TCATCAGAGG AAGAGGAAGG AGAGGTGGAC 
AGTGAAGTGG AACTGGCCTC AAGCCAGAGG TGGCCTCAGG GCCTGAAAAT GCGCCAGTCA 
CTATCTACCT TCAGCTCAGA GAATCCATCG GATGGGGAGG AGGGCACAGC TAGTGAGCCT 
TCCCCCAGTG GCACACCTGA AGTTGGCAGT ACCAACACAG ATGAGCGGCC GGATGAACGG 
TCTGATGACA TGTGCTCGCA GGGCTCAGAA ATCCCACTGG ACCCACCAGC TTCTGAGGTG 
GTTACTGGCC CAGAACCCAG CTCCTTGCCC GTCCCACACC ACGACCTACT CAGAGGGGAG 
CAGGGCCCTC CCAACCCTGA GGACTCAGAC TGTGACAGCA CTGAACTGGA CAACGCCAAC 
AGCGGTGAAG CCTTGCGGCC CCCAGCCTCC CTCCCTCCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Analysis of bovine mammary gland EST and functional annotation of the Bos taurus gene index.
Mammalian genome : official journal of the International Mammalian Genome Society13(7)373-9(2002 Jul)
Sonstegard TS,Capuco AV,White J,Van Tassell CP,Connor EE,Cho J,Sultana R,Shade L,Wray JE,Wells KD,Quackenbush J

Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries and construction of a gene index for cattle.
Genome research11(4)626-30(2001 Apr)
Smith TP,Grosse WM,Freking BA,Roberts AJ,Stone RT,Casas E,Wray JE,White J,Cho J,Fahrenkrug SC,Bennett GL,Heaton MP,Laegreid WW,Rohrer GA,Chitko-McKown CG,Pertea G,Holt I,Karamycheva S,Liang F,Quackenbush J,Keele JW

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MAP3K12 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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