FAM221A cDNA ORF clone, Bos taurus(cattle)

The following FAM221A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FAM221A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa39397 NM_001075195.2
Latest version!
Bos taurus family with sequence similarity 221, member A (FAM221A), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $174.30
$249.00
OBa18623 XM_005205512.4
Latest version!
Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $174.30
$249.00
OBa18624 XM_005205513.4
Latest version!
Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $174.30
$249.00
OBa18626 XM_005205515.4
Latest version!
Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $174.30
$249.00
OBa90628 XM_015469738.2
Latest version!
Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $174.30
$249.00
OBa18657 XM_010804343.2
Latest version!
Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa18657 XM_015469733.2
Latest version!
Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa18657 XM_015463070.1
Latest version!
Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa18657 XM_024990537.1
Latest version!
Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa233579 XM_024990534.1
Latest version!
Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa90622 XM_015469714.2
Latest version!
Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa90623 XM_015469715.2
Latest version!
Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa90624 XM_024990535.1
Latest version!
Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa90624 XM_015469716.2
Latest version!
Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa90625 XM_015469718.1
Latest version!
Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa90626 XM_015469719.2
Latest version!
Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa90626 XM_015469721.2
Latest version!
Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa90627 XM_015469729.1
Latest version!
Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa90627 XM_024990536.1
Latest version!
Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa90627 XM_015469725.2
Latest version!
Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa18655 XM_015463068.1
Latest version!
Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa18655 XM_005205559.4
Latest version!
Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa18656 XM_005205560.4
Latest version!
Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa18656 XM_015463069.1
Latest version!
Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
OBa90623 XM_015469715.1 Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa18623 XM_005205512.3 Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $174.30
$249.00
OBa90626 XM_015469721.1 Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa18624 XM_005205513.3 Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $174.30
$249.00
OBa18626 XM_005205515.3 Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $174.30
$249.00
OBa90622 XM_015469714.1 Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa90624 XM_015469716.1 Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa18656 XM_005205560.3 Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa18657 XM_015469733.1 Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa90626 XM_015469719.1 Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa90627 XM_015469725.1 Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa18655 XM_005205559.3 Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa90628 XM_015469738.1 Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $174.30
$249.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa39397
    Clone ID Related Accession (Same CDS sequence) NM_001075195.2
    Accession Version NM_001075195.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 801bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-13
    Organism Bos taurus(cattle)
    Product protein FAM221A
    Comment Comment: PREDICTED REFSEQ: This record has not been reviewed and the function is unknown. The reference sequence was derived from BC109687.1. On Aug 31, 2012 this sequence version replaced NM_001075195.1. ##Evidence-Data-START## Transcript exon combination :: BC109687.1, DT852302.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145420, SAMN03145437 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGGAGCGGC TGACGTTACC TCCTGGCGGC GCGGCGGCAG TGGACGAGTA CTTGGAGTAC 
    CGGAGAATTG TTGGTGAGGA TGACGGAGGG AAACTTTTTA CTCCTGAGGA ATATGAAGAA
    TACAAAAGAA AAGTCTTACC TATGCGTTTA CAGAACAGAT TGTATGTGAG TTGGCAGTCA
    CCCACGGGAA TGGATTGTAA ACTTGTGGGT CCAGAGACAC TGTGTTTTTG TACACATAGG
    TATAAGCAAC ATAAAACTGA CTTTGAAACG ATTCCCCCGC AGCGCCCCAT CAGTCTGCCT
    TGCCAAGTGA GGGGCTGCCC TTGCCGGGCT TACGTCTACG TGCCTCTGAA CGGCACACAG
    CCCATTCGCT GCAGATGTAA GCACTTTGCC GATCAACACA GTGCAGCGCC GGGCTTTGTA
    TGCAATGCCT GTTCTAAGTG TTCGGGATTT CATAGTTGCT TTACTTGTGC TTGTGGTCAG
    CCTGCATATG CCCATGACAC AGTAGTGGAA ACTAAGCAAG AAAGACTGGC TCAGGGAAAA
    CCAGTGGGGC AGGACGTTCC TTATGCAGCA ATGGGAGGAT TAACTGGCTT CAGCTCGCTG
    GCAGAAGGCT ACATACGATT AGATGACAGT GGAATTGGTG CACCATCAGC TGAATTTTTA
    GACTCTCCAG TTATGGCCAT GGACCACCCA TTTCTAAAGG CATTTCAAGC ATCATCTAGC
    TCTTCTCCAG AAACGCTAAC AGCCTTTCCA CAAAGAATTT TAAGGCTGCT TACAAAACAA
    CACACAATGA AGAAATTATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001068663.1
    CDS65..865
    Translation

    Target ORF information:

    RefSeq Version NM_001075195.2
    Organism Bos taurus(cattle)
    Definition Bos taurus family with sequence similarity 221, member A (FAM221A), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001075195.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGGAGCGGC TGACGTTACC TCCTGGCGGC GCGGCGGCAG TGGACGAGTA CTTGGAGTAC 
    CGGAGAATTG TTGGTGAGGA TGACGGAGGG AAACTTTTTA CTCCTGAGGA ATATGAAGAA
    TACAAAAGAA AAGTCTTACC TATGCGTTTA CAGAACAGAT TGTATGTGAG TTGGCAGTCA
    CCCACGGGAA TGGATTGTAA ACTTGTGGGT CCAGAGACAC TGTGTTTTTG TACACATAGG
    TATAAGCAAC ATAAAACTGA CTTTGAAACG ATTCCCCCGC AGCGCCCCAT CAGTCTGCCT
    TGCCAAGTGA GGGGCTGCCC TTGCCGGGCT TACGTCTACG TGCCTCTGAA CGGCACACAG
    CCCATTCGCT GCAGATGTAA GCACTTTGCC GATCAACACA GTGCAGCGCC GGGCTTTGTA
    TGCAATGCCT GTTCTAAGTG TTCGGGATTT CATAGTTGCT TTACTTGTGC TTGTGGTCAG
    CCTGCATATG CCCATGACAC AGTAGTGGAA ACTAAGCAAG AAAGACTGGC TCAGGGAAAA
    CCAGTGGGGC AGGACGTTCC TTATGCAGCA ATGGGAGGAT TAACTGGCTT CAGCTCGCTG
    GCAGAAGGCT ACATACGATT AGATGACAGT GGAATTGGTG CACCATCAGC TGAATTTTTA
    GACTCTCCAG TTATGGCCAT GGACCACCCA TTTCTAAAGG CATTTCAAGC ATCATCTAGC
    TCTTCTCCAG AAACGCTAAC AGCCTTTCCA CAAAGAATTT TAAGGCTGCT TACAAAACAA
    CACACAATGA AGAAATTATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18623
    Clone ID Related Accession (Same CDS sequence) XM_005205512.4 , XM_005205512.3
    Accession Version XM_005205512.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 894bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein FAM221A isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005205512.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGGAGCGGC TGACGTTACC TCCTGGCGGC GCGGCGGCAG TGGACGAGTA CTTGGAGTAC 
    CGGAGAATTG TTGGTGAGGA TGACGGAGGG AAACTTTTTA CTCCTGAGGA ATATGAAGAA
    TACAAAAGAA AAGTCTTACC TATGCGTTTA CAGAACAGAT TGTATGTGAG TTGGCAGTCA
    CCCACGGGAA TGGATTGTAA ACTTGTGGGT CCAGAGACAC TGTGTTTTTG TACACATAGG
    TATAAGCAAC ATAAAACTGA CTTTGAAACG ATTCCCCCGC AGCGCCCCAT CAGTCTGCCT
    TGCCAAGTGA GGGGCTGCCC TTGCCGGGCT TACGTCTACG TGCCTCTGAA CGGCACACAG
    CCCATTCGCT GCAGATGTAA GCACTTTGCC GATCAACACA GTGCAGCGCC GGGCTTTGTA
    TGCAATGCCT GTTCTAAGTG TTCGGGATTT CATAGTTGCT TTACTTGTGC TTGTGGTCAG
    CCTGCATATG CCCATGACAC AGTAGTGGAA ACTAAGCAAG AAAGACTGGC TCAGGGAAAA
    CCAGTGGGGC AGGACGTTCC TTATGCAGCA ATGGGAGGAT TAACTGGCTT CAGCTCGCTG
    GCAGAAGGCT ACATACGATT AGATGACAGT GGAATTGGTG CACCATCAGC TGAATTTTTA
    GACTCTCCAG TTATGGCCAT GGACCACCCA TTTCTAAAGG CATTTCAAGC ATCATCTAGC
    TCTTCTCCAG AAACGCTAAC AGTAGGTACA AGTAGTCAAG TTTCTTCCTT AAGGAAACCA
    GAAGAGGACG ATATGGCTTT CTTTGAAAGA CGATACCAGG AGAGGATAAA AATGGAAAAG
    GCTGCTAAGC AGAAAGGAAA AGCACCATTG CCATCAACTA TGAAACCTTC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005205569.1
    CDS90..983
    Translation

    Target ORF information:

    RefSeq Version XM_005205512.4
    Organism Bos taurus(cattle)
    Definition Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005205512.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGGAGCGGC TGACGTTACC TCCTGGCGGC GCGGCGGCAG TGGACGAGTA CTTGGAGTAC 
    CGGAGAATTG TTGGTGAGGA TGACGGAGGG AAACTTTTTA CTCCTGAGGA ATATGAAGAA
    TACAAAAGAA AAGTCTTACC TATGCGTTTA CAGAACAGAT TGTATGTGAG TTGGCAGTCA
    CCCACGGGAA TGGATTGTAA ACTTGTGGGT CCAGAGACAC TGTGTTTTTG TACACATAGG
    TATAAGCAAC ATAAAACTGA CTTTGAAACG ATTCCCCCGC AGCGCCCCAT CAGTCTGCCT
    TGCCAAGTGA GGGGCTGCCC TTGCCGGGCT TACGTCTACG TGCCTCTGAA CGGCACACAG
    CCCATTCGCT GCAGATGTAA GCACTTTGCC GATCAACACA GTGCAGCGCC GGGCTTTGTA
    TGCAATGCCT GTTCTAAGTG TTCGGGATTT CATAGTTGCT TTACTTGTGC TTGTGGTCAG
    CCTGCATATG CCCATGACAC AGTAGTGGAA ACTAAGCAAG AAAGACTGGC TCAGGGAAAA
    CCAGTGGGGC AGGACGTTCC TTATGCAGCA ATGGGAGGAT TAACTGGCTT CAGCTCGCTG
    GCAGAAGGCT ACATACGATT AGATGACAGT GGAATTGGTG CACCATCAGC TGAATTTTTA
    GACTCTCCAG TTATGGCCAT GGACCACCCA TTTCTAAAGG CATTTCAAGC ATCATCTAGC
    TCTTCTCCAG AAACGCTAAC AGTAGGTACA AGTAGTCAAG TTTCTTCCTT AAGGAAACCA
    GAAGAGGACG ATATGGCTTT CTTTGAAAGA CGATACCAGG AGAGGATAAA AATGGAAAAG
    GCTGCTAAGC AGAAAGGAAA AGCACCATTG CCATCAACTA TGAAACCTTC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18623
    Clone ID Related Accession (Same CDS sequence) XM_005205512.4 , XM_005205512.3
    Accession Version XM_005205512.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 894bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein FAM221A isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103900.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005205512.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGGAGCGGC TGACGTTACC TCCTGGCGGC GCGGCGGCAG TGGACGAGTA CTTGGAGTAC 
    CGGAGAATTG TTGGTGAGGA TGACGGAGGG AAACTTTTTA CTCCTGAGGA ATATGAAGAA
    TACAAAAGAA AAGTCTTACC TATGCGTTTA CAGAACAGAT TGTATGTGAG TTGGCAGTCA
    CCCACGGGAA TGGATTGTAA ACTTGTGGGT CCAGAGACAC TGTGTTTTTG TACACATAGG
    TATAAGCAAC ATAAAACTGA CTTTGAAACG ATTCCCCCGC AGCGCCCCAT CAGTCTGCCT
    TGCCAAGTGA GGGGCTGCCC TTGCCGGGCT TACGTCTACG TGCCTCTGAA CGGCACACAG
    CCCATTCGCT GCAGATGTAA GCACTTTGCC GATCAACACA GTGCAGCGCC GGGCTTTGTA
    TGCAATGCCT GTTCTAAGTG TTCGGGATTT CATAGTTGCT TTACTTGTGC TTGTGGTCAG
    CCTGCATATG CCCATGACAC AGTAGTGGAA ACTAAGCAAG AAAGACTGGC TCAGGGAAAA
    CCAGTGGGGC AGGACGTTCC TTATGCAGCA ATGGGAGGAT TAACTGGCTT CAGCTCGCTG
    GCAGAAGGCT ACATACGATT AGATGACAGT GGAATTGGTG CACCATCAGC TGAATTTTTA
    GACTCTCCAG TTATGGCCAT GGACCACCCA TTTCTAAAGG CATTTCAAGC ATCATCTAGC
    TCTTCTCCAG AAACGCTAAC AGTAGGTACA AGTAGTCAAG TTTCTTCCTT AAGGAAACCA
    GAAGAGGACG ATATGGCTTT CTTTGAAAGA CGATACCAGG AGAGGATAAA AATGGAAAAG
    GCTGCTAAGC AGAAAGGAAA AGCACCATTG CCATCAACTA TGAAACCTTC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005205569.1
    CDS76..969
    Translation

    Target ORF information:

    RefSeq Version XM_005205512.3
    Organism Bos taurus(cattle)
    Definition Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005205512.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGGAGCGGC TGACGTTACC TCCTGGCGGC GCGGCGGCAG TGGACGAGTA CTTGGAGTAC 
    CGGAGAATTG TTGGTGAGGA TGACGGAGGG AAACTTTTTA CTCCTGAGGA ATATGAAGAA
    TACAAAAGAA AAGTCTTACC TATGCGTTTA CAGAACAGAT TGTATGTGAG TTGGCAGTCA
    CCCACGGGAA TGGATTGTAA ACTTGTGGGT CCAGAGACAC TGTGTTTTTG TACACATAGG
    TATAAGCAAC ATAAAACTGA CTTTGAAACG ATTCCCCCGC AGCGCCCCAT CAGTCTGCCT
    TGCCAAGTGA GGGGCTGCCC TTGCCGGGCT TACGTCTACG TGCCTCTGAA CGGCACACAG
    CCCATTCGCT GCAGATGTAA GCACTTTGCC GATCAACACA GTGCAGCGCC GGGCTTTGTA
    TGCAATGCCT GTTCTAAGTG TTCGGGATTT CATAGTTGCT TTACTTGTGC TTGTGGTCAG
    CCTGCATATG CCCATGACAC AGTAGTGGAA ACTAAGCAAG AAAGACTGGC TCAGGGAAAA
    CCAGTGGGGC AGGACGTTCC TTATGCAGCA ATGGGAGGAT TAACTGGCTT CAGCTCGCTG
    GCAGAAGGCT ACATACGATT AGATGACAGT GGAATTGGTG CACCATCAGC TGAATTTTTA
    GACTCTCCAG TTATGGCCAT GGACCACCCA TTTCTAAAGG CATTTCAAGC ATCATCTAGC
    TCTTCTCCAG AAACGCTAAC AGTAGGTACA AGTAGTCAAG TTTCTTCCTT AAGGAAACCA
    GAAGAGGACG ATATGGCTTT CTTTGAAAGA CGATACCAGG AGAGGATAAA AATGGAAAAG
    GCTGCTAAGC AGAAAGGAAA AGCACCATTG CCATCAACTA TGAAACCTTC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18624
    Clone ID Related Accession (Same CDS sequence) XM_005205513.4 , XM_005205513.3
    Accession Version XM_005205513.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 891bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein FAM221A isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005205513.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGGAGCGGC TGACGTTACC TCCTGGCGGC GCGGCGGCAG TGGACGAGTA CTTGGAGTAC 
    CGGAGAATTG TTGGTGAGGA TGACGGAGGG AAACTTTTTA CTCCTGAGGA ATATGAAGAA
    TACAAAAGAA AAGTCTTACC TATGCGTTTA CAGAACAGAT TGTATGTGAG TTGGCAGTCA
    CCCACGGGAA TGGATTGTAA ACTTGTGGGT CCAGAGACAC TGTGTTTTTG TACACATAGG
    TATAAGCAAC ATAAAACTGA CTTTGAAACG ATTCCCCCGC AGCGCCCCAT CAGTCTGCCT
    TGCCAAGTGA GGGGCTGCCC TTGCCGGGCT TACGTCTACG TGCCTCTGAA CGGCACACAG
    CCCATTCGCT GCAGATGTAA GCACTTTGCC GATCAACACA GTGCAGCGCC GGGCTTTGTA
    TGCAATGCCT GTTCTAAGTG TTCGGGATTT CATAGTTGCT TTACTTGTGC TTGTGGTCAG
    CCTGCATATG CCCATGACAC AGTAGTGGAA ACTAAGCAAG AAAGACTGGC TCAGGGAAAA
    CCAGTGGGGC AGGACGTTCC TTATGCAGCA ATGGGAGGAT TAACTGGCTT CAGCTCGCTG
    GCAGAAGGCT ACATACGATT AGATGACAGT GGAATTGGTG CACCATCAGC TGAATTTTTA
    GACTCTCCAG TTATGGCCAT GGACCACCCA TTTCTAAAGG CATTTCAAGC ATCATCTAGC
    TCTTCTCCAG AAACGCTAAC AGGTACAAGT AGTCAAGTTT CTTCCTTAAG GAAACCAGAA
    GAGGACGATA TGGCTTTCTT TGAAAGACGA TACCAGGAGA GGATAAAAAT GGAAAAGGCT
    GCTAAGCAGA AAGGAAAAGC ACCATTGCCA TCAACTATGA AACCTTCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005205570.1
    CDS95..985
    Translation

    Target ORF information:

    RefSeq Version XM_005205513.4
    Organism Bos taurus(cattle)
    Definition Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005205513.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGGAGCGGC TGACGTTACC TCCTGGCGGC GCGGCGGCAG TGGACGAGTA CTTGGAGTAC 
    CGGAGAATTG TTGGTGAGGA TGACGGAGGG AAACTTTTTA CTCCTGAGGA ATATGAAGAA
    TACAAAAGAA AAGTCTTACC TATGCGTTTA CAGAACAGAT TGTATGTGAG TTGGCAGTCA
    CCCACGGGAA TGGATTGTAA ACTTGTGGGT CCAGAGACAC TGTGTTTTTG TACACATAGG
    TATAAGCAAC ATAAAACTGA CTTTGAAACG ATTCCCCCGC AGCGCCCCAT CAGTCTGCCT
    TGCCAAGTGA GGGGCTGCCC TTGCCGGGCT TACGTCTACG TGCCTCTGAA CGGCACACAG
    CCCATTCGCT GCAGATGTAA GCACTTTGCC GATCAACACA GTGCAGCGCC GGGCTTTGTA
    TGCAATGCCT GTTCTAAGTG TTCGGGATTT CATAGTTGCT TTACTTGTGC TTGTGGTCAG
    CCTGCATATG CCCATGACAC AGTAGTGGAA ACTAAGCAAG AAAGACTGGC TCAGGGAAAA
    CCAGTGGGGC AGGACGTTCC TTATGCAGCA ATGGGAGGAT TAACTGGCTT CAGCTCGCTG
    GCAGAAGGCT ACATACGATT AGATGACAGT GGAATTGGTG CACCATCAGC TGAATTTTTA
    GACTCTCCAG TTATGGCCAT GGACCACCCA TTTCTAAAGG CATTTCAAGC ATCATCTAGC
    TCTTCTCCAG AAACGCTAAC AGGTACAAGT AGTCAAGTTT CTTCCTTAAG GAAACCAGAA
    GAGGACGATA TGGCTTTCTT TGAAAGACGA TACCAGGAGA GGATAAAAAT GGAAAAGGCT
    GCTAAGCAGA AAGGAAAAGC ACCATTGCCA TCAACTATGA AACCTTCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18624
    Clone ID Related Accession (Same CDS sequence) XM_005205513.4 , XM_005205513.3
    Accession Version XM_005205513.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 891bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein FAM221A isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103900.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005205513.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGGAGCGGC TGACGTTACC TCCTGGCGGC GCGGCGGCAG TGGACGAGTA CTTGGAGTAC 
    CGGAGAATTG TTGGTGAGGA TGACGGAGGG AAACTTTTTA CTCCTGAGGA ATATGAAGAA
    TACAAAAGAA AAGTCTTACC TATGCGTTTA CAGAACAGAT TGTATGTGAG TTGGCAGTCA
    CCCACGGGAA TGGATTGTAA ACTTGTGGGT CCAGAGACAC TGTGTTTTTG TACACATAGG
    TATAAGCAAC ATAAAACTGA CTTTGAAACG ATTCCCCCGC AGCGCCCCAT CAGTCTGCCT
    TGCCAAGTGA GGGGCTGCCC TTGCCGGGCT TACGTCTACG TGCCTCTGAA CGGCACACAG
    CCCATTCGCT GCAGATGTAA GCACTTTGCC GATCAACACA GTGCAGCGCC GGGCTTTGTA
    TGCAATGCCT GTTCTAAGTG TTCGGGATTT CATAGTTGCT TTACTTGTGC TTGTGGTCAG
    CCTGCATATG CCCATGACAC AGTAGTGGAA ACTAAGCAAG AAAGACTGGC TCAGGGAAAA
    CCAGTGGGGC AGGACGTTCC TTATGCAGCA ATGGGAGGAT TAACTGGCTT CAGCTCGCTG
    GCAGAAGGCT ACATACGATT AGATGACAGT GGAATTGGTG CACCATCAGC TGAATTTTTA
    GACTCTCCAG TTATGGCCAT GGACCACCCA TTTCTAAAGG CATTTCAAGC ATCATCTAGC
    TCTTCTCCAG AAACGCTAAC AGGTACAAGT AGTCAAGTTT CTTCCTTAAG GAAACCAGAA
    GAGGACGATA TGGCTTTCTT TGAAAGACGA TACCAGGAGA GGATAAAAAT GGAAAAGGCT
    GCTAAGCAGA AAGGAAAAGC ACCATTGCCA TCAACTATGA AACCTTCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005205570.1
    CDS78..968
    Translation

    Target ORF information:

    RefSeq Version XM_005205513.3
    Organism Bos taurus(cattle)
    Definition Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005205513.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGGAGCGGC TGACGTTACC TCCTGGCGGC GCGGCGGCAG TGGACGAGTA CTTGGAGTAC 
    CGGAGAATTG TTGGTGAGGA TGACGGAGGG AAACTTTTTA CTCCTGAGGA ATATGAAGAA
    TACAAAAGAA AAGTCTTACC TATGCGTTTA CAGAACAGAT TGTATGTGAG TTGGCAGTCA
    CCCACGGGAA TGGATTGTAA ACTTGTGGGT CCAGAGACAC TGTGTTTTTG TACACATAGG
    TATAAGCAAC ATAAAACTGA CTTTGAAACG ATTCCCCCGC AGCGCCCCAT CAGTCTGCCT
    TGCCAAGTGA GGGGCTGCCC TTGCCGGGCT TACGTCTACG TGCCTCTGAA CGGCACACAG
    CCCATTCGCT GCAGATGTAA GCACTTTGCC GATCAACACA GTGCAGCGCC GGGCTTTGTA
    TGCAATGCCT GTTCTAAGTG TTCGGGATTT CATAGTTGCT TTACTTGTGC TTGTGGTCAG
    CCTGCATATG CCCATGACAC AGTAGTGGAA ACTAAGCAAG AAAGACTGGC TCAGGGAAAA
    CCAGTGGGGC AGGACGTTCC TTATGCAGCA ATGGGAGGAT TAACTGGCTT CAGCTCGCTG
    GCAGAAGGCT ACATACGATT AGATGACAGT GGAATTGGTG CACCATCAGC TGAATTTTTA
    GACTCTCCAG TTATGGCCAT GGACCACCCA TTTCTAAAGG CATTTCAAGC ATCATCTAGC
    TCTTCTCCAG AAACGCTAAC AGGTACAAGT AGTCAAGTTT CTTCCTTAAG GAAACCAGAA
    GAGGACGATA TGGCTTTCTT TGAAAGACGA TACCAGGAGA GGATAAAAAT GGAAAAGGCT
    GCTAAGCAGA AAGGAAAAGC ACCATTGCCA TCAACTATGA AACCTTCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18626
    Clone ID Related Accession (Same CDS sequence) XM_005205515.4 , XM_005205515.3
    Accession Version XM_005205515.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 843bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein FAM221A isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005205515.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGGAGCGGC TGACGTTACC TCCTGGCGGC GCGGCGGCAG TGGACGAGTA CTTGGAGTAC 
    CGGAGAATTG TTGGTGAGGA TGACGGAGGG AAACTTTTTA CTCCTGAGGA ATATGAAGAA
    TACAAAAGAA AAGTCTTACC TATGCGTTTA CAGAACAGAT TGTATGTGAG TTGGCAGTCA
    CCCACGGGAA TGGATTGTAA ACTTGTGGGT CCAGAGACAC TGTGTTTTTG TACACATAGG
    TATAAGCAAC ATAAAACTGA CTTTGAAACG ATTCCCCCGC AGCGCCCCAT CAGTCTGCCT
    TGCCAAGTGA GGGGCTGCCC TTGCCGGGCT TACGTCTACG TGCCTCTGAA CGGCACACAG
    CCCATTCGCT GCAGATGTAA GCACTTTGCC GATCAACACA GTGCAGCGCC GGGCTTTGTA
    TGCAATGCCT GTTCTAAGTG TTCGGGATTT CATAGTTGCT TTACTTGTGC TTGTGGTCAG
    CCTGCATATG CCCATGACAC AGTAGTGGAA ACTAAGCAAG AAAGACTGGC TCAGGGAAAA
    CCAGTGGGGC AGGACGTTCC TTATGCAGCA ATGGGAGGAT TAACTGGCTT CAGCTCGCTG
    GCAGAAGGCT ACATACGATT AGATGACAGT GGAATTGGTG CACCATCAGC TGAATTTTTA
    GACTCTCCAG TTATGGCCAT GGACCACCCA TTTCTAAAGG CATTTCAAGC ATCATCTAGC
    TCTTCTCCAG AAACGCTAAC AGTAGGTACA AGTAGTCAAG TTTCTTCCTT AAGGAAACCA
    GAAGAGGACG ATATGGCTTT CTTTGAAAGA CGATACCAGG AGAGGGAACT TGGGCAGTGT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005205572.1
    CDS93..935
    Translation

    Target ORF information:

    RefSeq Version XM_005205515.4
    Organism Bos taurus(cattle)
    Definition Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005205515.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGGAGCGGC TGACGTTACC TCCTGGCGGC GCGGCGGCAG TGGACGAGTA CTTGGAGTAC 
    CGGAGAATTG TTGGTGAGGA TGACGGAGGG AAACTTTTTA CTCCTGAGGA ATATGAAGAA
    TACAAAAGAA AAGTCTTACC TATGCGTTTA CAGAACAGAT TGTATGTGAG TTGGCAGTCA
    CCCACGGGAA TGGATTGTAA ACTTGTGGGT CCAGAGACAC TGTGTTTTTG TACACATAGG
    TATAAGCAAC ATAAAACTGA CTTTGAAACG ATTCCCCCGC AGCGCCCCAT CAGTCTGCCT
    TGCCAAGTGA GGGGCTGCCC TTGCCGGGCT TACGTCTACG TGCCTCTGAA CGGCACACAG
    CCCATTCGCT GCAGATGTAA GCACTTTGCC GATCAACACA GTGCAGCGCC GGGCTTTGTA
    TGCAATGCCT GTTCTAAGTG TTCGGGATTT CATAGTTGCT TTACTTGTGC TTGTGGTCAG
    CCTGCATATG CCCATGACAC AGTAGTGGAA ACTAAGCAAG AAAGACTGGC TCAGGGAAAA
    CCAGTGGGGC AGGACGTTCC TTATGCAGCA ATGGGAGGAT TAACTGGCTT CAGCTCGCTG
    GCAGAAGGCT ACATACGATT AGATGACAGT GGAATTGGTG CACCATCAGC TGAATTTTTA
    GACTCTCCAG TTATGGCCAT GGACCACCCA TTTCTAAAGG CATTTCAAGC ATCATCTAGC
    TCTTCTCCAG AAACGCTAAC AGTAGGTACA AGTAGTCAAG TTTCTTCCTT AAGGAAACCA
    GAAGAGGACG ATATGGCTTT CTTTGAAAGA CGATACCAGG AGAGGGAACT TGGGCAGTGT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18626
    Clone ID Related Accession (Same CDS sequence) XM_005205515.4 , XM_005205515.3
    Accession Version XM_005205515.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 843bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein FAM221A isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103900.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005205515.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGGAGCGGC TGACGTTACC TCCTGGCGGC GCGGCGGCAG TGGACGAGTA CTTGGAGTAC 
    CGGAGAATTG TTGGTGAGGA TGACGGAGGG AAACTTTTTA CTCCTGAGGA ATATGAAGAA
    TACAAAAGAA AAGTCTTACC TATGCGTTTA CAGAACAGAT TGTATGTGAG TTGGCAGTCA
    CCCACGGGAA TGGATTGTAA ACTTGTGGGT CCAGAGACAC TGTGTTTTTG TACACATAGG
    TATAAGCAAC ATAAAACTGA CTTTGAAACG ATTCCCCCGC AGCGCCCCAT CAGTCTGCCT
    TGCCAAGTGA GGGGCTGCCC TTGCCGGGCT TACGTCTACG TGCCTCTGAA CGGCACACAG
    CCCATTCGCT GCAGATGTAA GCACTTTGCC GATCAACACA GTGCAGCGCC GGGCTTTGTA
    TGCAATGCCT GTTCTAAGTG TTCGGGATTT CATAGTTGCT TTACTTGTGC TTGTGGTCAG
    CCTGCATATG CCCATGACAC AGTAGTGGAA ACTAAGCAAG AAAGACTGGC TCAGGGAAAA
    CCAGTGGGGC AGGACGTTCC TTATGCAGCA ATGGGAGGAT TAACTGGCTT CAGCTCGCTG
    GCAGAAGGCT ACATACGATT AGATGACAGT GGAATTGGTG CACCATCAGC TGAATTTTTA
    GACTCTCCAG TTATGGCCAT GGACCACCCA TTTCTAAAGG CATTTCAAGC ATCATCTAGC
    TCTTCTCCAG AAACGCTAAC AGTAGGTACA AGTAGTCAAG TTTCTTCCTT AAGGAAACCA
    GAAGAGGACG ATATGGCTTT CTTTGAAAGA CGATACCAGG AGAGGGAACT TGGGCAGTGT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005205572.1
    CDS78..920
    Translation

    Target ORF information:

    RefSeq Version XM_005205515.3
    Organism Bos taurus(cattle)
    Definition Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005205515.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGGAGCGGC TGACGTTACC TCCTGGCGGC GCGGCGGCAG TGGACGAGTA CTTGGAGTAC 
    CGGAGAATTG TTGGTGAGGA TGACGGAGGG AAACTTTTTA CTCCTGAGGA ATATGAAGAA
    TACAAAAGAA AAGTCTTACC TATGCGTTTA CAGAACAGAT TGTATGTGAG TTGGCAGTCA
    CCCACGGGAA TGGATTGTAA ACTTGTGGGT CCAGAGACAC TGTGTTTTTG TACACATAGG
    TATAAGCAAC ATAAAACTGA CTTTGAAACG ATTCCCCCGC AGCGCCCCAT CAGTCTGCCT
    TGCCAAGTGA GGGGCTGCCC TTGCCGGGCT TACGTCTACG TGCCTCTGAA CGGCACACAG
    CCCATTCGCT GCAGATGTAA GCACTTTGCC GATCAACACA GTGCAGCGCC GGGCTTTGTA
    TGCAATGCCT GTTCTAAGTG TTCGGGATTT CATAGTTGCT TTACTTGTGC TTGTGGTCAG
    CCTGCATATG CCCATGACAC AGTAGTGGAA ACTAAGCAAG AAAGACTGGC TCAGGGAAAA
    CCAGTGGGGC AGGACGTTCC TTATGCAGCA ATGGGAGGAT TAACTGGCTT CAGCTCGCTG
    GCAGAAGGCT ACATACGATT AGATGACAGT GGAATTGGTG CACCATCAGC TGAATTTTTA
    GACTCTCCAG TTATGGCCAT GGACCACCCA TTTCTAAAGG CATTTCAAGC ATCATCTAGC
    TCTTCTCCAG AAACGCTAAC AGTAGGTACA AGTAGTCAAG TTTCTTCCTT AAGGAAACCA
    GAAGAGGACG ATATGGCTTT CTTTGAAAGA CGATACCAGG AGAGGGAACT TGGGCAGTGT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90628
    Clone ID Related Accession (Same CDS sequence) XM_015469738.1 , XM_015469738.2
    Accession Version XM_015469738.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 828bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein FAM221A isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103900.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGGAGCGGC TGACGTTACC TCCTGGCGGC GCGGCGGCAG TGGACGAGTA CTTGGAGTAC 
    CGGAGAATTG TTGGTGAGGA TGACGGAGGG AAACTTTTTA CTCCTGAGGA ATATGAAGAA
    TACAAAAGAA AAGTCTTACC TATGCGTTTA CAGAACAGAT TGTATGTGAG TTGGCAGTCA
    CCCACGGGAA TGGATTGTAA ACTTGTGGGT CCAGAGACAC TGTGTTTTTG TACACATAGG
    TATAAGCAAC ATAAAACTGA CTTTGAAACG ATTCCCCCGC AGCGCCCCAT CAGTCTGCCT
    TGCCAAGTGA GGGGCTGCCC TTGCCGGGCT TACGTCTACG TGCCTCTGAA CGGCACACAG
    CCCATTCGCT GCAGATGTAA GCACTTTGCC GATCAACACA GTGCAGCGCC GGGCTTTGTA
    TGCAATGCCT GTTCTAAGTG TTCGGGATTT CATAGTTGCT TTACTTGTGC TTGTGGTCAG
    CCTGCATATG CCCATGACAC AGTAGTGGAA ACTAAGCAAG AAAGACTGGC TCAGGGAAAA
    CCAGTGGGGC AGGACGTTCC TTATGCAGCA ATGGGAGGAT TAACTGGCTT CAGCTCGCTG
    GCAGAAGGCT ACATACGATT AGATGACAGT GGAATTGGTG CACCATCAGC TGAATTTTTA
    GACTCTCCAG TTATGGCCAT GGACCACCCA TTTCTAAAGG CATTTCAAGC ATCATCTAGC
    TCTTCTCCAG AAACGCTAAC AGTTTTGCAG GAATTGTCCA AATGGAGGAA GATAAACATT
    GCAATAAAGT TGAAGATGTG GTTGGAAGTC ATGTGGAAGA TGCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015325224.1
    CDS80..907
    Translation

    Target ORF information:

    RefSeq Version XM_015469738.1
    Organism Bos taurus(cattle)
    Definition Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015469738.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGGAGCGGC TGACGTTACC TCCTGGCGGC GCGGCGGCAG TGGACGAGTA CTTGGAGTAC 
    CGGAGAATTG TTGGTGAGGA TGACGGAGGG AAACTTTTTA CTCCTGAGGA ATATGAAGAA
    TACAAAAGAA AAGTCTTACC TATGCGTTTA CAGAACAGAT TGTATGTGAG TTGGCAGTCA
    CCCACGGGAA TGGATTGTAA ACTTGTGGGT CCAGAGACAC TGTGTTTTTG TACACATAGG
    TATAAGCAAC ATAAAACTGA CTTTGAAACG ATTCCCCCGC AGCGCCCCAT CAGTCTGCCT
    TGCCAAGTGA GGGGCTGCCC TTGCCGGGCT TACGTCTACG TGCCTCTGAA CGGCACACAG
    CCCATTCGCT GCAGATGTAA GCACTTTGCC GATCAACACA GTGCAGCGCC GGGCTTTGTA
    TGCAATGCCT GTTCTAAGTG TTCGGGATTT CATAGTTGCT TTACTTGTGC TTGTGGTCAG
    CCTGCATATG CCCATGACAC AGTAGTGGAA ACTAAGCAAG AAAGACTGGC TCAGGGAAAA
    CCAGTGGGGC AGGACGTTCC TTATGCAGCA ATGGGAGGAT TAACTGGCTT CAGCTCGCTG
    GCAGAAGGCT ACATACGATT AGATGACAGT GGAATTGGTG CACCATCAGC TGAATTTTTA
    GACTCTCCAG TTATGGCCAT GGACCACCCA TTTCTAAAGG CATTTCAAGC ATCATCTAGC
    TCTTCTCCAG AAACGCTAAC AGTTTTGCAG GAATTGTCCA AATGGAGGAA GATAAACATT
    GCAATAAAGT TGAAGATGTG GTTGGAAGTC ATGTGGAAGA TGCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90628
    Clone ID Related Accession (Same CDS sequence) XM_015469738.1 , XM_015469738.2
    Accession Version XM_015469738.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 828bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein FAM221A isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015469738.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGGAGCGGC TGACGTTACC TCCTGGCGGC GCGGCGGCAG TGGACGAGTA CTTGGAGTAC 
    CGGAGAATTG TTGGTGAGGA TGACGGAGGG AAACTTTTTA CTCCTGAGGA ATATGAAGAA
    TACAAAAGAA AAGTCTTACC TATGCGTTTA CAGAACAGAT TGTATGTGAG TTGGCAGTCA
    CCCACGGGAA TGGATTGTAA ACTTGTGGGT CCAGAGACAC TGTGTTTTTG TACACATAGG
    TATAAGCAAC ATAAAACTGA CTTTGAAACG ATTCCCCCGC AGCGCCCCAT CAGTCTGCCT
    TGCCAAGTGA GGGGCTGCCC TTGCCGGGCT TACGTCTACG TGCCTCTGAA CGGCACACAG
    CCCATTCGCT GCAGATGTAA GCACTTTGCC GATCAACACA GTGCAGCGCC GGGCTTTGTA
    TGCAATGCCT GTTCTAAGTG TTCGGGATTT CATAGTTGCT TTACTTGTGC TTGTGGTCAG
    CCTGCATATG CCCATGACAC AGTAGTGGAA ACTAAGCAAG AAAGACTGGC TCAGGGAAAA
    CCAGTGGGGC AGGACGTTCC TTATGCAGCA ATGGGAGGAT TAACTGGCTT CAGCTCGCTG
    GCAGAAGGCT ACATACGATT AGATGACAGT GGAATTGGTG CACCATCAGC TGAATTTTTA
    GACTCTCCAG TTATGGCCAT GGACCACCCA TTTCTAAAGG CATTTCAAGC ATCATCTAGC
    TCTTCTCCAG AAACGCTAAC AGTTTTGCAG GAATTGTCCA AATGGAGGAA GATAAACATT
    GCAATAAAGT TGAAGATGTG GTTGGAAGTC ATGTGGAAGA TGCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015325224.1
    CDS569..1396
    Translation

    Target ORF information:

    RefSeq Version XM_015469738.2
    Organism Bos taurus(cattle)
    Definition Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015469738.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGGAGCGGC TGACGTTACC TCCTGGCGGC GCGGCGGCAG TGGACGAGTA CTTGGAGTAC 
    CGGAGAATTG TTGGTGAGGA TGACGGAGGG AAACTTTTTA CTCCTGAGGA ATATGAAGAA
    TACAAAAGAA AAGTCTTACC TATGCGTTTA CAGAACAGAT TGTATGTGAG TTGGCAGTCA
    CCCACGGGAA TGGATTGTAA ACTTGTGGGT CCAGAGACAC TGTGTTTTTG TACACATAGG
    TATAAGCAAC ATAAAACTGA CTTTGAAACG ATTCCCCCGC AGCGCCCCAT CAGTCTGCCT
    TGCCAAGTGA GGGGCTGCCC TTGCCGGGCT TACGTCTACG TGCCTCTGAA CGGCACACAG
    CCCATTCGCT GCAGATGTAA GCACTTTGCC GATCAACACA GTGCAGCGCC GGGCTTTGTA
    TGCAATGCCT GTTCTAAGTG TTCGGGATTT CATAGTTGCT TTACTTGTGC TTGTGGTCAG
    CCTGCATATG CCCATGACAC AGTAGTGGAA ACTAAGCAAG AAAGACTGGC TCAGGGAAAA
    CCAGTGGGGC AGGACGTTCC TTATGCAGCA ATGGGAGGAT TAACTGGCTT CAGCTCGCTG
    GCAGAAGGCT ACATACGATT AGATGACAGT GGAATTGGTG CACCATCAGC TGAATTTTTA
    GACTCTCCAG TTATGGCCAT GGACCACCCA TTTCTAAAGG CATTTCAAGC ATCATCTAGC
    TCTTCTCCAG AAACGCTAAC AGTTTTGCAG GAATTGTCCA AATGGAGGAA GATAAACATT
    GCAATAAAGT TGAAGATGTG GTTGGAAGTC ATGTGGAAGA TGCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18657
    Clone ID Related Accession (Same CDS sequence) XM_015463070.1 , XM_015469733.1 , XM_015469733.2 , XM_010804343.2 , XM_024990537.1
    Accession Version XM_015463070.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2988bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein FAM221A isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644725.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGAGGAAG ATAAACATTG CAATAAAGTT GAAGATGTGG TTGGAAGTCA TGTGGAAGAT 
    GCAGTAACAT TTTGGGCCCA GAGTATCAAT AGAAATAAGG ATATTATGAA GATTGGTTGT
    TCACTGTCTG AAGTCTGTCC CCTTGCCAAT TCAGTGTTTG GGAATCTTGA TCCAAAGAAG
    ATTTATGGTG GATTATTTTC TGAAGATAAA TGTTGGTACA GATGCCAAGT ACTGAAAGTC
    ATCAACGATG AAAAGTGTCT GGTGAGATAT ATTGACTATG GAAATACAGA AATTCTGAAT
    CGATCGGATA TAGTAGAGAT TCCTTTGGAT CTGCAATTTT CTAGTGTTGC CAAAAAGTAT
    AGACTTTGGG GACTACAGAT TCCTTCTAGC CAAGAAGTTA CCCAGTTTGA TCAGGGCAGA
    ACCTTTTTGG GGAGCTTGAT TTTTGAAAAG GAAATTAAAA TGAGAATTAA AGCTACCTAT
    CAAGATGGAA CAATAATTGC TCAGGCTGAG TATGGCAATG TAGATATTGG GGAAGAGGTG
    GCTAAGAAAG GATTTGCAGA AAAATGCAGA CTCACTTCCA GCACTAATAT CTGTGAGGAA
    AAAAAATCAG ATCCTGGTCA GCTTGTCCTC AGGAACCTCA AAAGCCCCAT TCCTTTGTGG
    GGGCATAGAT CAAGCCAATC AAACTTCACC CGGCCAAAGG GGCGCTTAAG TGGGAGAATG
    TGTCTTGACT TGAAGAATGA AAATGATGCA GGAAATAATG TGACATTTCC AAAGGAAAAT
    TTGGCTGTTG GTGACTTTAA TTTAGGGTCT AATGTCAGCC TGGCAAAAAT TAAGCAGGAC
    CAGAAACTGA TTGAAGAAAA TGAAAAACTT AAAACAGAGA AGGAGGTTCT TCTTGAAAGG
    TGCAAGGCAT TAGAATTGAA AGTCGAACAG ACTGCCCAGG AGCTGCAGCA AGAGAAAGCA
    ACTACCATGG ATTTGACTAA ACGTTTGGAA AGCACTGTGA AGACTTGTAT AGGTACCAGA
    ATGAAAAATC TGGCAGCTAA GGTTGAAATA TTGAAAGAAA TTAGACGTGT CAGTATCAGT
    GTTCGTTTTG GAAATGACCT TTCAGATGCT ATACAAGTGT TGGATGAAGA CTGCTTTACT
    ATCCCAGCTT CTTTGAATGA ATTAGAGCTA ATTTGGGCAG AATATAATCT GGCTCAGGAG
    AAGATTCAAA CTTGTGAGAA TGTGAATGAA GGGAATATTT TGATTGCTGA AAGAAATGAA
    GTACAACAGA AGCTGTACAT GGCAGTAGAA GTTTTTATTC TGGAAGTTGA TGAGTTATCT
    CTTAATAAAC GCTTAAAAAC GTTGCAGGAT TTGTCAGCCT CTTTAGAAGC AGTATATGGA
    CAGGCCAAAG AAGGAATTAA CTCTGAAGAA ATACTGGAGA AATTTAATGA CTGGAGGTGT
    AATAAAAGAG AGGAGTTCAC CAGTGTTAGA AGTGAAACAG AGACTTGTAT ACAACGTCTT
    ATAGCATGGT TCCAGAATAC TTTAAAGGCC TTTGATCTGT CTGTGGACAA ATCACTGATT
    TCTGATGACA CAATTGATAA TATTGATGAA ATCCTGGAGA ACACTGAGTC AGGTGTCTGC
    AAGGAGCTGG AGATATCTCT GATTGAACAA GGTGATGCAG ACAAGGAGAT CATTTTAAAA
    ACATGTAGTC AAGTACTACA AAAGATCCGT TCAGAGGAAA GCCTTATTGC CACAGTACAA
    TCCAAGTACA AGGACAGTGT TGAGTTTAAG AAACAGATTA TTGAATGTTT AAATAAGAGC
    CCCAGCGTGG ATCACTTGCT TTCCATTAAA AAGACACTGA AAAGCTTAAA GGCTCGACTG
    AGATGGAAAT TGGTTGAAAA GAATAATTTG GAAGAATCTG ATGACCATGA TGGATCTGAG
    ATTGAAAAAA TAAAACAAGA AATAACTCAG TTACGGAATA GTGTCTTTGA GGGAATTTAT
    CATGAGCAGG AGGAATATGC GAAGCTGAGT AGCTTAGTAC AAAAATGGTT CCCTGAGCTT
    CCTCTACTTC ATCCTGAAAT AGGATTACTT AAGTACATGA ACTCTGGTGG TCTTCTTACA
    ATGAGCTTGG AAAGAGATCT TCTTGATGCT GAGCCTATGA AGGAGCTTAG CAGCAAGCGT
    CCTTTGATAT GTTCTGAGGT TAATGGGCAG ATAATTCTTT TAAAGGGCTA TTCTGTGGAT
    GTCGACACAG AAGCCAGGGT AATTCAGAGA GCAGCTGCCT ATCACACAGC TTGGAGAGAA
    GCTAAAGGAG AATCTGGGTT GCTACCACTG ATATTCTTAT TTTTGTGTAA GTCGGATCCT
    ATGGCTTATC TGATGGTCCC ATATTATCCT AGGGCAAATC TGAATGCTGT TCAGTCCACC
    ATGCCTTTAA CTTCAGAAGA AGTTTTAAAG GTCATGAAAG GTGTAGCCCA GGGCCTTCAT
    ACATTGCACA AGGCTGACAT AGTTCATGGA TCACTTCATC AGAACAATGT TTTTGCTTTA
    AACCGTCAAC ATGGAATTGT TGGAGATTTT GACTTCACCA AATCTGTGAG TCAGCGAACC
    TCAGTGAACA TGATGGTTGG TGACTTAAGT TTGCTATCAC CTGAACTGAA AATGGGAAAA
    CCTGCTTCTC CGAGTTCTGA CTTATATGCT TATGGCTGCC TTTTATTGTG GCTTTCAGTT
    CAGAATCAGG AATTTGAAAC TAATGAGGAT GGAATCCCAA AGATGGATCA GTTTCATTTG
    GATGATAATG TCAAATCCCT CCTCTGTAGC TTGATCTGTT GTAGAAGTTC AATGACTGCC
    GAGCAGGTTT TGAATGCTGA CTGTTTCTTG CTACCAAAGG GAAAATCAAT GCCAAACTCA
    GAGAAAGATA CTGAATACAT CCCCAAAAAA GAGGAAGAAG AATTAAAGGT GAAGAACTTG
    GATAAAGATA AGGAAAAGAC AAGAAATGGT GAAGCCAGCT TTGATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015318556.1
    CDS1325..4312
    Translation

    Target ORF information:

    RefSeq Version XM_015463070.1
    Organism Bos taurus(cattle)
    Definition Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463070.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGAGGAAG ATAAACATTG CAATAAAGTT GAAGATGTGG TTGGAAGTCA TGTGGAAGAT 
    GCAGTAACAT TTTGGGCCCA GAGTATCAAT AGAAATAAGG ATATTATGAA GATTGGTTGT
    TCACTGTCTG AAGTCTGTCC CCTTGCCAAT TCAGTGTTTG GGAATCTTGA TCCAAAGAAG
    ATTTATGGTG GATTATTTTC TGAAGATAAA TGTTGGTACA GATGCCAAGT ACTGAAAGTC
    ATCAACGATG AAAAGTGTCT GGTGAGATAT ATTGACTATG GAAATACAGA AATTCTGAAT
    CGATCGGATA TAGTAGAGAT TCCTTTGGAT CTGCAATTTT CTAGTGTTGC CAAAAAGTAT
    AGACTTTGGG GACTACAGAT TCCTTCTAGC CAAGAAGTTA CCCAGTTTGA TCAGGGCAGA
    ACCTTTTTGG GGAGCTTGAT TTTTGAAAAG GAAATTAAAA TGAGAATTAA AGCTACCTAT
    CAAGATGGAA CAATAATTGC TCAGGCTGAG TATGGCAATG TAGATATTGG GGAAGAGGTG
    GCTAAGAAAG GATTTGCAGA AAAATGCAGA CTCACTTCCA GCACTAATAT CTGTGAGGAA
    AAAAAATCAG ATCCTGGTCA GCTTGTCCTC AGGAACCTCA AAAGCCCCAT TCCTTTGTGG
    GGGCATAGAT CAAGCCAATC AAACTTCACC CGGCCAAAGG GGCGCTTAAG TGGGAGAATG
    TGTCTTGACT TGAAGAATGA AAATGATGCA GGAAATAATG TGACATTTCC AAAGGAAAAT
    TTGGCTGTTG GTGACTTTAA TTTAGGGTCT AATGTCAGCC TGGCAAAAAT TAAGCAGGAC
    CAGAAACTGA TTGAAGAAAA TGAAAAACTT AAAACAGAGA AGGAGGTTCT TCTTGAAAGG
    TGCAAGGCAT TAGAATTGAA AGTCGAACAG ACTGCCCAGG AGCTGCAGCA AGAGAAAGCA
    ACTACCATGG ATTTGACTAA ACGTTTGGAA AGCACTGTGA AGACTTGTAT AGGTACCAGA
    ATGAAAAATC TGGCAGCTAA GGTTGAAATA TTGAAAGAAA TTAGACGTGT CAGTATCAGT
    GTTCGTTTTG GAAATGACCT TTCAGATGCT ATACAAGTGT TGGATGAAGA CTGCTTTACT
    ATCCCAGCTT CTTTGAATGA ATTAGAGCTA ATTTGGGCAG AATATAATCT GGCTCAGGAG
    AAGATTCAAA CTTGTGAGAA TGTGAATGAA GGGAATATTT TGATTGCTGA AAGAAATGAA
    GTACAACAGA AGCTGTACAT GGCAGTAGAA GTTTTTATTC TGGAAGTTGA TGAGTTATCT
    CTTAATAAAC GCTTAAAAAC GTTGCAGGAT TTGTCAGCCT CTTTAGAAGC AGTATATGGA
    CAGGCCAAAG AAGGAATTAA CTCTGAAGAA ATACTGGAGA AATTTAATGA CTGGAGGTGT
    AATAAAAGAG AGGAGTTCAC CAGTGTTAGA AGTGAAACAG AGACTTGTAT ACAACGTCTT
    ATAGCATGGT TCCAGAATAC TTTAAAGGCC TTTGATCTGT CTGTGGACAA ATCACTGATT
    TCTGATGACA CAATTGATAA TATTGATGAA ATCCTGGAGA ACACTGAGTC AGGTGTCTGC
    AAGGAGCTGG AGATATCTCT GATTGAACAA GGTGATGCAG ACAAGGAGAT CATTTTAAAA
    ACATGTAGTC AAGTACTACA AAAGATCCGT TCAGAGGAAA GCCTTATTGC CACAGTACAA
    TCCAAGTACA AGGACAGTGT TGAGTTTAAG AAACAGATTA TTGAATGTTT AAATAAGAGC
    CCCAGCGTGG ATCACTTGCT TTCCATTAAA AAGACACTGA AAAGCTTAAA GGCTCGACTG
    AGATGGAAAT TGGTTGAAAA GAATAATTTG GAAGAATCTG ATGACCATGA TGGATCTGAG
    ATTGAAAAAA TAAAACAAGA AATAACTCAG TTACGGAATA GTGTCTTTGA GGGAATTTAT
    CATGAGCAGG AGGAATATGC GAAGCTGAGT AGCTTAGTAC AAAAATGGTT CCCTGAGCTT
    CCTCTACTTC ATCCTGAAAT AGGATTACTT AAGTACATGA ACTCTGGTGG TCTTCTTACA
    ATGAGCTTGG AAAGAGATCT TCTTGATGCT GAGCCTATGA AGGAGCTTAG CAGCAAGCGT
    CCTTTGATAT GTTCTGAGGT TAATGGGCAG ATAATTCTTT TAAAGGGCTA TTCTGTGGAT
    GTCGACACAG AAGCCAGGGT AATTCAGAGA GCAGCTGCCT ATCACACAGC TTGGAGAGAA
    GCTAAAGGAG AATCTGGGTT GCTACCACTG ATATTCTTAT TTTTGTGTAA GTCGGATCCT
    ATGGCTTATC TGATGGTCCC ATATTATCCT AGGGCAAATC TGAATGCTGT TCAGTCCACC
    ATGCCTTTAA CTTCAGAAGA AGTTTTAAAG GTCATGAAAG GTGTAGCCCA GGGCCTTCAT
    ACATTGCACA AGGCTGACAT AGTTCATGGA TCACTTCATC AGAACAATGT TTTTGCTTTA
    AACCGTCAAC ATGGAATTGT TGGAGATTTT GACTTCACCA AATCTGTGAG TCAGCGAACC
    TCAGTGAACA TGATGGTTGG TGACTTAAGT TTGCTATCAC CTGAACTGAA AATGGGAAAA
    CCTGCTTCTC CGAGTTCTGA CTTATATGCT TATGGCTGCC TTTTATTGTG GCTTTCAGTT
    CAGAATCAGG AATTTGAAAC TAATGAGGAT GGAATCCCAA AGATGGATCA GTTTCATTTG
    GATGATAATG TCAAATCCCT CCTCTGTAGC TTGATCTGTT GTAGAAGTTC AATGACTGCC
    GAGCAGGTTT TGAATGCTGA CTGTTTCTTG CTACCAAAGG GAAAATCAAT GCCAAACTCA
    GAGAAAGATA CTGAATACAT CCCCAAAAAA GAGGAAGAAG AATTAAAGGT GAAGAACTTG
    GATAAAGATA AGGAAAAGAC AAGAAATGGT GAAGCCAGCT TTGATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18657
    Clone ID Related Accession (Same CDS sequence) XM_015463070.1 , XM_015469733.1 , XM_015469733.2 , XM_010804343.2 , XM_024990537.1
    Accession Version XM_015469733.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2988bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein FAM221A isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103900.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGAGGAAG ATAAACATTG CAATAAAGTT GAAGATGTGG TTGGAAGTCA TGTGGAAGAT 
    GCAGTAACAT TTTGGGCCCA GAGTATCAAT AGAAATAAGG ATATTATGAA GATTGGTTGT
    TCACTGTCTG AAGTCTGTCC CCTTGCCAAT TCAGTGTTTG GGAATCTTGA TCCAAAGAAG
    ATTTATGGTG GATTATTTTC TGAAGATAAA TGTTGGTACA GATGCCAAGT ACTGAAAGTC
    ATCAACGATG AAAAGTGTCT GGTGAGATAT ATTGACTATG GAAATACAGA AATTCTGAAT
    CGATCGGATA TAGTAGAGAT TCCTTTGGAT CTGCAATTTT CTAGTGTTGC CAAAAAGTAT
    AGACTTTGGG GACTACAGAT TCCTTCTAGC CAAGAAGTTA CCCAGTTTGA TCAGGGCAGA
    ACCTTTTTGG GGAGCTTGAT TTTTGAAAAG GAAATTAAAA TGAGAATTAA AGCTACCTAT
    CAAGATGGAA CAATAATTGC TCAGGCTGAG TATGGCAATG TAGATATTGG GGAAGAGGTG
    GCTAAGAAAG GATTTGCAGA AAAATGCAGA CTCACTTCCA GCACTAATAT CTGTGAGGAA
    AAAAAATCAG ATCCTGGTCA GCTTGTCCTC AGGAACCTCA AAAGCCCCAT TCCTTTGTGG
    GGGCATAGAT CAAGCCAATC AAACTTCACC CGGCCAAAGG GGCGCTTAAG TGGGAGAATG
    TGTCTTGACT TGAAGAATGA AAATGATGCA GGAAATAATG TGACATTTCC AAAGGAAAAT
    TTGGCTGTTG GTGACTTTAA TTTAGGGTCT AATGTCAGCC TGGCAAAAAT TAAGCAGGAC
    CAGAAACTGA TTGAAGAAAA TGAAAAACTT AAAACAGAGA AGGAGGTTCT TCTTGAAAGG
    TGCAAGGCAT TAGAATTGAA AGTCGAACAG ACTGCCCAGG AGCTGCAGCA AGAGAAAGCA
    ACTACCATGG ATTTGACTAA ACGTTTGGAA AGCACTGTGA AGACTTGTAT AGGTACCAGA
    ATGAAAAATC TGGCAGCTAA GGTTGAAATA TTGAAAGAAA TTAGACGTGT CAGTATCAGT
    GTTCGTTTTG GAAATGACCT TTCAGATGCT ATACAAGTGT TGGATGAAGA CTGCTTTACT
    ATCCCAGCTT CTTTGAATGA ATTAGAGCTA ATTTGGGCAG AATATAATCT GGCTCAGGAG
    AAGATTCAAA CTTGTGAGAA TGTGAATGAA GGGAATATTT TGATTGCTGA AAGAAATGAA
    GTACAACAGA AGCTGTACAT GGCAGTAGAA GTTTTTATTC TGGAAGTTGA TGAGTTATCT
    CTTAATAAAC GCTTAAAAAC GTTGCAGGAT TTGTCAGCCT CTTTAGAAGC AGTATATGGA
    CAGGCCAAAG AAGGAATTAA CTCTGAAGAA ATACTGGAGA AATTTAATGA CTGGAGGTGT
    AATAAAAGAG AGGAGTTCAC CAGTGTTAGA AGTGAAACAG AGACTTGTAT ACAACGTCTT
    ATAGCATGGT TCCAGAATAC TTTAAAGGCC TTTGATCTGT CTGTGGACAA ATCACTGATT
    TCTGATGACA CAATTGATAA TATTGATGAA ATCCTGGAGA ACACTGAGTC AGGTGTCTGC
    AAGGAGCTGG AGATATCTCT GATTGAACAA GGTGATGCAG ACAAGGAGAT CATTTTAAAA
    ACATGTAGTC AAGTACTACA AAAGATCCGT TCAGAGGAAA GCCTTATTGC CACAGTACAA
    TCCAAGTACA AGGACAGTGT TGAGTTTAAG AAACAGATTA TTGAATGTTT AAATAAGAGC
    CCCAGCGTGG ATCACTTGCT TTCCATTAAA AAGACACTGA AAAGCTTAAA GGCTCGACTG
    AGATGGAAAT TGGTTGAAAA GAATAATTTG GAAGAATCTG ATGACCATGA TGGATCTGAG
    ATTGAAAAAA TAAAACAAGA AATAACTCAG TTACGGAATA GTGTCTTTGA GGGAATTTAT
    CATGAGCAGG AGGAATATGC GAAGCTGAGT AGCTTAGTAC AAAAATGGTT CCCTGAGCTT
    CCTCTACTTC ATCCTGAAAT AGGATTACTT AAGTACATGA ACTCTGGTGG TCTTCTTACA
    ATGAGCTTGG AAAGAGATCT TCTTGATGCT GAGCCTATGA AGGAGCTTAG CAGCAAGCGT
    CCTTTGATAT GTTCTGAGGT TAATGGGCAG ATAATTCTTT TAAAGGGCTA TTCTGTGGAT
    GTCGACACAG AAGCCAGGGT AATTCAGAGA GCAGCTGCCT ATCACACAGC TTGGAGAGAA
    GCTAAAGGAG AATCTGGGTT GCTACCACTG ATATTCTTAT TTTTGTGTAA GTCGGATCCT
    ATGGCTTATC TGATGGTCCC ATATTATCCT AGGGCAAATC TGAATGCTGT TCAGTCCACC
    ATGCCTTTAA CTTCAGAAGA AGTTTTAAAG GTCATGAAAG GTGTAGCCCA GGGCCTTCAT
    ACATTGCACA AGGCTGACAT AGTTCATGGA TCACTTCATC AGAACAATGT TTTTGCTTTA
    AACCGTCAAC ATGGAATTGT TGGAGATTTT GACTTCACCA AATCTGTGAG TCAGCGAACC
    TCAGTGAACA TGATGGTTGG TGACTTAAGT TTGCTATCAC CTGAACTGAA AATGGGAAAA
    CCTGCTTCTC CGAGTTCTGA CTTATATGCT TATGGCTGCC TTTTATTGTG GCTTTCAGTT
    CAGAATCAGG AATTTGAAAC TAATGAGGAT GGAATCCCAA AGATGGATCA GTTTCATTTG
    GATGATAATG TCAAATCCCT CCTCTGTAGC TTGATCTGTT GTAGAAGTTC AATGACTGCC
    GAGCAGGTTT TGAATGCTGA CTGTTTCTTG CTACCAAAGG GAAAATCAAT GCCAAACTCA
    GAGAAAGATA CTGAATACAT CCCCAAAAAA GAGGAAGAAG AATTAAAGGT GAAGAACTTG
    GATAAAGATA AGGAAAAGAC AAGAAATGGT GAAGCCAGCT TTGATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015325219.1
    CDS115..3102
    Translation

    Target ORF information:

    RefSeq Version XM_015469733.1
    Organism Bos taurus(cattle)
    Definition Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015469733.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGAGGAAG ATAAACATTG CAATAAAGTT GAAGATGTGG TTGGAAGTCA TGTGGAAGAT 
    GCAGTAACAT TTTGGGCCCA GAGTATCAAT AGAAATAAGG ATATTATGAA GATTGGTTGT
    TCACTGTCTG AAGTCTGTCC CCTTGCCAAT TCAGTGTTTG GGAATCTTGA TCCAAAGAAG
    ATTTATGGTG GATTATTTTC TGAAGATAAA TGTTGGTACA GATGCCAAGT ACTGAAAGTC
    ATCAACGATG AAAAGTGTCT GGTGAGATAT ATTGACTATG GAAATACAGA AATTCTGAAT
    CGATCGGATA TAGTAGAGAT TCCTTTGGAT CTGCAATTTT CTAGTGTTGC CAAAAAGTAT
    AGACTTTGGG GACTACAGAT TCCTTCTAGC CAAGAAGTTA CCCAGTTTGA TCAGGGCAGA
    ACCTTTTTGG GGAGCTTGAT TTTTGAAAAG GAAATTAAAA TGAGAATTAA AGCTACCTAT
    CAAGATGGAA CAATAATTGC TCAGGCTGAG TATGGCAATG TAGATATTGG GGAAGAGGTG
    GCTAAGAAAG GATTTGCAGA AAAATGCAGA CTCACTTCCA GCACTAATAT CTGTGAGGAA
    AAAAAATCAG ATCCTGGTCA GCTTGTCCTC AGGAACCTCA AAAGCCCCAT TCCTTTGTGG
    GGGCATAGAT CAAGCCAATC AAACTTCACC CGGCCAAAGG GGCGCTTAAG TGGGAGAATG
    TGTCTTGACT TGAAGAATGA AAATGATGCA GGAAATAATG TGACATTTCC AAAGGAAAAT
    TTGGCTGTTG GTGACTTTAA TTTAGGGTCT AATGTCAGCC TGGCAAAAAT TAAGCAGGAC
    CAGAAACTGA TTGAAGAAAA TGAAAAACTT AAAACAGAGA AGGAGGTTCT TCTTGAAAGG
    TGCAAGGCAT TAGAATTGAA AGTCGAACAG ACTGCCCAGG AGCTGCAGCA AGAGAAAGCA
    ACTACCATGG ATTTGACTAA ACGTTTGGAA AGCACTGTGA AGACTTGTAT AGGTACCAGA
    ATGAAAAATC TGGCAGCTAA GGTTGAAATA TTGAAAGAAA TTAGACGTGT CAGTATCAGT
    GTTCGTTTTG GAAATGACCT TTCAGATGCT ATACAAGTGT TGGATGAAGA CTGCTTTACT
    ATCCCAGCTT CTTTGAATGA ATTAGAGCTA ATTTGGGCAG AATATAATCT GGCTCAGGAG
    AAGATTCAAA CTTGTGAGAA TGTGAATGAA GGGAATATTT TGATTGCTGA AAGAAATGAA
    GTACAACAGA AGCTGTACAT GGCAGTAGAA GTTTTTATTC TGGAAGTTGA TGAGTTATCT
    CTTAATAAAC GCTTAAAAAC GTTGCAGGAT TTGTCAGCCT CTTTAGAAGC AGTATATGGA
    CAGGCCAAAG AAGGAATTAA CTCTGAAGAA ATACTGGAGA AATTTAATGA CTGGAGGTGT
    AATAAAAGAG AGGAGTTCAC CAGTGTTAGA AGTGAAACAG AGACTTGTAT ACAACGTCTT
    ATAGCATGGT TCCAGAATAC TTTAAAGGCC TTTGATCTGT CTGTGGACAA ATCACTGATT
    TCTGATGACA CAATTGATAA TATTGATGAA ATCCTGGAGA ACACTGAGTC AGGTGTCTGC
    AAGGAGCTGG AGATATCTCT GATTGAACAA GGTGATGCAG ACAAGGAGAT CATTTTAAAA
    ACATGTAGTC AAGTACTACA AAAGATCCGT TCAGAGGAAA GCCTTATTGC CACAGTACAA
    TCCAAGTACA AGGACAGTGT TGAGTTTAAG AAACAGATTA TTGAATGTTT AAATAAGAGC
    CCCAGCGTGG ATCACTTGCT TTCCATTAAA AAGACACTGA AAAGCTTAAA GGCTCGACTG
    AGATGGAAAT TGGTTGAAAA GAATAATTTG GAAGAATCTG ATGACCATGA TGGATCTGAG
    ATTGAAAAAA TAAAACAAGA AATAACTCAG TTACGGAATA GTGTCTTTGA GGGAATTTAT
    CATGAGCAGG AGGAATATGC GAAGCTGAGT AGCTTAGTAC AAAAATGGTT CCCTGAGCTT
    CCTCTACTTC ATCCTGAAAT AGGATTACTT AAGTACATGA ACTCTGGTGG TCTTCTTACA
    ATGAGCTTGG AAAGAGATCT TCTTGATGCT GAGCCTATGA AGGAGCTTAG CAGCAAGCGT
    CCTTTGATAT GTTCTGAGGT TAATGGGCAG ATAATTCTTT TAAAGGGCTA TTCTGTGGAT
    GTCGACACAG AAGCCAGGGT AATTCAGAGA GCAGCTGCCT ATCACACAGC TTGGAGAGAA
    GCTAAAGGAG AATCTGGGTT GCTACCACTG ATATTCTTAT TTTTGTGTAA GTCGGATCCT
    ATGGCTTATC TGATGGTCCC ATATTATCCT AGGGCAAATC TGAATGCTGT TCAGTCCACC
    ATGCCTTTAA CTTCAGAAGA AGTTTTAAAG GTCATGAAAG GTGTAGCCCA GGGCCTTCAT
    ACATTGCACA AGGCTGACAT AGTTCATGGA TCACTTCATC AGAACAATGT TTTTGCTTTA
    AACCGTCAAC ATGGAATTGT TGGAGATTTT GACTTCACCA AATCTGTGAG TCAGCGAACC
    TCAGTGAACA TGATGGTTGG TGACTTAAGT TTGCTATCAC CTGAACTGAA AATGGGAAAA
    CCTGCTTCTC CGAGTTCTGA CTTATATGCT TATGGCTGCC TTTTATTGTG GCTTTCAGTT
    CAGAATCAGG AATTTGAAAC TAATGAGGAT GGAATCCCAA AGATGGATCA GTTTCATTTG
    GATGATAATG TCAAATCCCT CCTCTGTAGC TTGATCTGTT GTAGAAGTTC AATGACTGCC
    GAGCAGGTTT TGAATGCTGA CTGTTTCTTG CTACCAAAGG GAAAATCAAT GCCAAACTCA
    GAGAAAGATA CTGAATACAT CCCCAAAAAA GAGGAAGAAG AATTAAAGGT GAAGAACTTG
    GATAAAGATA AGGAAAAGAC AAGAAATGGT GAAGCCAGCT TTGATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18657
    Clone ID Related Accession (Same CDS sequence) XM_015463070.1 , XM_015469733.1 , XM_015469733.2 , XM_010804343.2 , XM_024990537.1
    Accession Version XM_015469733.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2988bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein FAM221A isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015469733.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGAGGAAG ATAAACATTG CAATAAAGTT GAAGATGTGG TTGGAAGTCA TGTGGAAGAT 
    GCAGTAACAT TTTGGGCCCA GAGTATCAAT AGAAATAAGG ATATTATGAA GATTGGTTGT
    TCACTGTCTG AAGTCTGTCC CCTTGCCAAT TCAGTGTTTG GGAATCTTGA TCCAAAGAAG
    ATTTATGGTG GATTATTTTC TGAAGATAAA TGTTGGTACA GATGCCAAGT ACTGAAAGTC
    ATCAACGATG AAAAGTGTCT GGTGAGATAT ATTGACTATG GAAATACAGA AATTCTGAAT
    CGATCGGATA TAGTAGAGAT TCCTTTGGAT CTGCAATTTT CTAGTGTTGC CAAAAAGTAT
    AGACTTTGGG GACTACAGAT TCCTTCTAGC CAAGAAGTTA CCCAGTTTGA TCAGGGCAGA
    ACCTTTTTGG GGAGCTTGAT TTTTGAAAAG GAAATTAAAA TGAGAATTAA AGCTACCTAT
    CAAGATGGAA CAATAATTGC TCAGGCTGAG TATGGCAATG TAGATATTGG GGAAGAGGTG
    GCTAAGAAAG GATTTGCAGA AAAATGCAGA CTCACTTCCA GCACTAATAT CTGTGAGGAA
    AAAAAATCAG ATCCTGGTCA GCTTGTCCTC AGGAACCTCA AAAGCCCCAT TCCTTTGTGG
    GGGCATAGAT CAAGCCAATC AAACTTCACC CGGCCAAAGG GGCGCTTAAG TGGGAGAATG
    TGTCTTGACT TGAAGAATGA AAATGATGCA GGAAATAATG TGACATTTCC AAAGGAAAAT
    TTGGCTGTTG GTGACTTTAA TTTAGGGTCT AATGTCAGCC TGGCAAAAAT TAAGCAGGAC
    CAGAAACTGA TTGAAGAAAA TGAAAAACTT AAAACAGAGA AGGAGGTTCT TCTTGAAAGG
    TGCAAGGCAT TAGAATTGAA AGTCGAACAG ACTGCCCAGG AGCTGCAGCA AGAGAAAGCA
    ACTACCATGG ATTTGACTAA ACGTTTGGAA AGCACTGTGA AGACTTGTAT AGGTACCAGA
    ATGAAAAATC TGGCAGCTAA GGTTGAAATA TTGAAAGAAA TTAGACGTGT CAGTATCAGT
    GTTCGTTTTG GAAATGACCT TTCAGATGCT ATACAAGTGT TGGATGAAGA CTGCTTTACT
    ATCCCAGCTT CTTTGAATGA ATTAGAGCTA ATTTGGGCAG AATATAATCT GGCTCAGGAG
    AAGATTCAAA CTTGTGAGAA TGTGAATGAA GGGAATATTT TGATTGCTGA AAGAAATGAA
    GTACAACAGA AGCTGTACAT GGCAGTAGAA GTTTTTATTC TGGAAGTTGA TGAGTTATCT
    CTTAATAAAC GCTTAAAAAC GTTGCAGGAT TTGTCAGCCT CTTTAGAAGC AGTATATGGA
    CAGGCCAAAG AAGGAATTAA CTCTGAAGAA ATACTGGAGA AATTTAATGA CTGGAGGTGT
    AATAAAAGAG AGGAGTTCAC CAGTGTTAGA AGTGAAACAG AGACTTGTAT ACAACGTCTT
    ATAGCATGGT TCCAGAATAC TTTAAAGGCC TTTGATCTGT CTGTGGACAA ATCACTGATT
    TCTGATGACA CAATTGATAA TATTGATGAA ATCCTGGAGA ACACTGAGTC AGGTGTCTGC
    AAGGAGCTGG AGATATCTCT GATTGAACAA GGTGATGCAG ACAAGGAGAT CATTTTAAAA
    ACATGTAGTC AAGTACTACA AAAGATCCGT TCAGAGGAAA GCCTTATTGC CACAGTACAA
    TCCAAGTACA AGGACAGTGT TGAGTTTAAG AAACAGATTA TTGAATGTTT AAATAAGAGC
    CCCAGCGTGG ATCACTTGCT TTCCATTAAA AAGACACTGA AAAGCTTAAA GGCTCGACTG
    AGATGGAAAT TGGTTGAAAA GAATAATTTG GAAGAATCTG ATGACCATGA TGGATCTGAG
    ATTGAAAAAA TAAAACAAGA AATAACTCAG TTACGGAATA GTGTCTTTGA GGGAATTTAT
    CATGAGCAGG AGGAATATGC GAAGCTGAGT AGCTTAGTAC AAAAATGGTT CCCTGAGCTT
    CCTCTACTTC ATCCTGAAAT AGGATTACTT AAGTACATGA ACTCTGGTGG TCTTCTTACA
    ATGAGCTTGG AAAGAGATCT TCTTGATGCT GAGCCTATGA AGGAGCTTAG CAGCAAGCGT
    CCTTTGATAT GTTCTGAGGT TAATGGGCAG ATAATTCTTT TAAAGGGCTA TTCTGTGGAT
    GTCGACACAG AAGCCAGGGT AATTCAGAGA GCAGCTGCCT ATCACACAGC TTGGAGAGAA
    GCTAAAGGAG AATCTGGGTT GCTACCACTG ATATTCTTAT TTTTGTGTAA GTCGGATCCT
    ATGGCTTATC TGATGGTCCC ATATTATCCT AGGGCAAATC TGAATGCTGT TCAGTCCACC
    ATGCCTTTAA CTTCAGAAGA AGTTTTAAAG GTCATGAAAG GTGTAGCCCA GGGCCTTCAT
    ACATTGCACA AGGCTGACAT AGTTCATGGA TCACTTCATC AGAACAATGT TTTTGCTTTA
    AACCGTCAAC ATGGAATTGT TGGAGATTTT GACTTCACCA AATCTGTGAG TCAGCGAACC
    TCAGTGAACA TGATGGTTGG TGACTTAAGT TTGCTATCAC CTGAACTGAA AATGGGAAAA
    CCTGCTTCTC CGAGTTCTGA CTTATATGCT TATGGCTGCC TTTTATTGTG GCTTTCAGTT
    CAGAATCAGG AATTTGAAAC TAATGAGGAT GGAATCCCAA AGATGGATCA GTTTCATTTG
    GATGATAATG TCAAATCCCT CCTCTGTAGC TTGATCTGTT GTAGAAGTTC AATGACTGCC
    GAGCAGGTTT TGAATGCTGA CTGTTTCTTG CTACCAAAGG GAAAATCAAT GCCAAACTCA
    GAGAAAGATA CTGAATACAT CCCCAAAAAA GAGGAAGAAG AATTAAAGGT GAAGAACTTG
    GATAAAGATA AGGAAAAGAC AAGAAATGGT GAAGCCAGCT TTGATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015325219.1
    CDS125..3112
    Translation

    Target ORF information:

    RefSeq Version XM_015469733.2
    Organism Bos taurus(cattle)
    Definition Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015469733.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGAGGAAG ATAAACATTG CAATAAAGTT GAAGATGTGG TTGGAAGTCA TGTGGAAGAT 
    GCAGTAACAT TTTGGGCCCA GAGTATCAAT AGAAATAAGG ATATTATGAA GATTGGTTGT
    TCACTGTCTG AAGTCTGTCC CCTTGCCAAT TCAGTGTTTG GGAATCTTGA TCCAAAGAAG
    ATTTATGGTG GATTATTTTC TGAAGATAAA TGTTGGTACA GATGCCAAGT ACTGAAAGTC
    ATCAACGATG AAAAGTGTCT GGTGAGATAT ATTGACTATG GAAATACAGA AATTCTGAAT
    CGATCGGATA TAGTAGAGAT TCCTTTGGAT CTGCAATTTT CTAGTGTTGC CAAAAAGTAT
    AGACTTTGGG GACTACAGAT TCCTTCTAGC CAAGAAGTTA CCCAGTTTGA TCAGGGCAGA
    ACCTTTTTGG GGAGCTTGAT TTTTGAAAAG GAAATTAAAA TGAGAATTAA AGCTACCTAT
    CAAGATGGAA CAATAATTGC TCAGGCTGAG TATGGCAATG TAGATATTGG GGAAGAGGTG
    GCTAAGAAAG GATTTGCAGA AAAATGCAGA CTCACTTCCA GCACTAATAT CTGTGAGGAA
    AAAAAATCAG ATCCTGGTCA GCTTGTCCTC AGGAACCTCA AAAGCCCCAT TCCTTTGTGG
    GGGCATAGAT CAAGCCAATC AAACTTCACC CGGCCAAAGG GGCGCTTAAG TGGGAGAATG
    TGTCTTGACT TGAAGAATGA AAATGATGCA GGAAATAATG TGACATTTCC AAAGGAAAAT
    TTGGCTGTTG GTGACTTTAA TTTAGGGTCT AATGTCAGCC TGGCAAAAAT TAAGCAGGAC
    CAGAAACTGA TTGAAGAAAA TGAAAAACTT AAAACAGAGA AGGAGGTTCT TCTTGAAAGG
    TGCAAGGCAT TAGAATTGAA AGTCGAACAG ACTGCCCAGG AGCTGCAGCA AGAGAAAGCA
    ACTACCATGG ATTTGACTAA ACGTTTGGAA AGCACTGTGA AGACTTGTAT AGGTACCAGA
    ATGAAAAATC TGGCAGCTAA GGTTGAAATA TTGAAAGAAA TTAGACGTGT CAGTATCAGT
    GTTCGTTTTG GAAATGACCT TTCAGATGCT ATACAAGTGT TGGATGAAGA CTGCTTTACT
    ATCCCAGCTT CTTTGAATGA ATTAGAGCTA ATTTGGGCAG AATATAATCT GGCTCAGGAG
    AAGATTCAAA CTTGTGAGAA TGTGAATGAA GGGAATATTT TGATTGCTGA AAGAAATGAA
    GTACAACAGA AGCTGTACAT GGCAGTAGAA GTTTTTATTC TGGAAGTTGA TGAGTTATCT
    CTTAATAAAC GCTTAAAAAC GTTGCAGGAT TTGTCAGCCT CTTTAGAAGC AGTATATGGA
    CAGGCCAAAG AAGGAATTAA CTCTGAAGAA ATACTGGAGA AATTTAATGA CTGGAGGTGT
    AATAAAAGAG AGGAGTTCAC CAGTGTTAGA AGTGAAACAG AGACTTGTAT ACAACGTCTT
    ATAGCATGGT TCCAGAATAC TTTAAAGGCC TTTGATCTGT CTGTGGACAA ATCACTGATT
    TCTGATGACA CAATTGATAA TATTGATGAA ATCCTGGAGA ACACTGAGTC AGGTGTCTGC
    AAGGAGCTGG AGATATCTCT GATTGAACAA GGTGATGCAG ACAAGGAGAT CATTTTAAAA
    ACATGTAGTC AAGTACTACA AAAGATCCGT TCAGAGGAAA GCCTTATTGC CACAGTACAA
    TCCAAGTACA AGGACAGTGT TGAGTTTAAG AAACAGATTA TTGAATGTTT AAATAAGAGC
    CCCAGCGTGG ATCACTTGCT TTCCATTAAA AAGACACTGA AAAGCTTAAA GGCTCGACTG
    AGATGGAAAT TGGTTGAAAA GAATAATTTG GAAGAATCTG ATGACCATGA TGGATCTGAG
    ATTGAAAAAA TAAAACAAGA AATAACTCAG TTACGGAATA GTGTCTTTGA GGGAATTTAT
    CATGAGCAGG AGGAATATGC GAAGCTGAGT AGCTTAGTAC AAAAATGGTT CCCTGAGCTT
    CCTCTACTTC ATCCTGAAAT AGGATTACTT AAGTACATGA ACTCTGGTGG TCTTCTTACA
    ATGAGCTTGG AAAGAGATCT TCTTGATGCT GAGCCTATGA AGGAGCTTAG CAGCAAGCGT
    CCTTTGATAT GTTCTGAGGT TAATGGGCAG ATAATTCTTT TAAAGGGCTA TTCTGTGGAT
    GTCGACACAG AAGCCAGGGT AATTCAGAGA GCAGCTGCCT ATCACACAGC TTGGAGAGAA
    GCTAAAGGAG AATCTGGGTT GCTACCACTG ATATTCTTAT TTTTGTGTAA GTCGGATCCT
    ATGGCTTATC TGATGGTCCC ATATTATCCT AGGGCAAATC TGAATGCTGT TCAGTCCACC
    ATGCCTTTAA CTTCAGAAGA AGTTTTAAAG GTCATGAAAG GTGTAGCCCA GGGCCTTCAT
    ACATTGCACA AGGCTGACAT AGTTCATGGA TCACTTCATC AGAACAATGT TTTTGCTTTA
    AACCGTCAAC ATGGAATTGT TGGAGATTTT GACTTCACCA AATCTGTGAG TCAGCGAACC
    TCAGTGAACA TGATGGTTGG TGACTTAAGT TTGCTATCAC CTGAACTGAA AATGGGAAAA
    CCTGCTTCTC CGAGTTCTGA CTTATATGCT TATGGCTGCC TTTTATTGTG GCTTTCAGTT
    CAGAATCAGG AATTTGAAAC TAATGAGGAT GGAATCCCAA AGATGGATCA GTTTCATTTG
    GATGATAATG TCAAATCCCT CCTCTGTAGC TTGATCTGTT GTAGAAGTTC AATGACTGCC
    GAGCAGGTTT TGAATGCTGA CTGTTTCTTG CTACCAAAGG GAAAATCAAT GCCAAACTCA
    GAGAAAGATA CTGAATACAT CCCCAAAAAA GAGGAAGAAG AATTAAAGGT GAAGAACTTG
    GATAAAGATA AGGAAAAGAC AAGAAATGGT GAAGCCAGCT TTGATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18657
    Clone ID Related Accession (Same CDS sequence) XM_015463070.1 , XM_015469733.1 , XM_015469733.2 , XM_010804343.2 , XM_024990537.1
    Accession Version XM_010804343.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2988bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein FAM221A isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103900.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010804343.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGAGGAAG ATAAACATTG CAATAAAGTT GAAGATGTGG TTGGAAGTCA TGTGGAAGAT 
    GCAGTAACAT TTTGGGCCCA GAGTATCAAT AGAAATAAGG ATATTATGAA GATTGGTTGT
    TCACTGTCTG AAGTCTGTCC CCTTGCCAAT TCAGTGTTTG GGAATCTTGA TCCAAAGAAG
    ATTTATGGTG GATTATTTTC TGAAGATAAA TGTTGGTACA GATGCCAAGT ACTGAAAGTC
    ATCAACGATG AAAAGTGTCT GGTGAGATAT ATTGACTATG GAAATACAGA AATTCTGAAT
    CGATCGGATA TAGTAGAGAT TCCTTTGGAT CTGCAATTTT CTAGTGTTGC CAAAAAGTAT
    AGACTTTGGG GACTACAGAT TCCTTCTAGC CAAGAAGTTA CCCAGTTTGA TCAGGGCAGA
    ACCTTTTTGG GGAGCTTGAT TTTTGAAAAG GAAATTAAAA TGAGAATTAA AGCTACCTAT
    CAAGATGGAA CAATAATTGC TCAGGCTGAG TATGGCAATG TAGATATTGG GGAAGAGGTG
    GCTAAGAAAG GATTTGCAGA AAAATGCAGA CTCACTTCCA GCACTAATAT CTGTGAGGAA
    AAAAAATCAG ATCCTGGTCA GCTTGTCCTC AGGAACCTCA AAAGCCCCAT TCCTTTGTGG
    GGGCATAGAT CAAGCCAATC AAACTTCACC CGGCCAAAGG GGCGCTTAAG TGGGAGAATG
    TGTCTTGACT TGAAGAATGA AAATGATGCA GGAAATAATG TGACATTTCC AAAGGAAAAT
    TTGGCTGTTG GTGACTTTAA TTTAGGGTCT AATGTCAGCC TGGCAAAAAT TAAGCAGGAC
    CAGAAACTGA TTGAAGAAAA TGAAAAACTT AAAACAGAGA AGGAGGTTCT TCTTGAAAGG
    TGCAAGGCAT TAGAATTGAA AGTCGAACAG ACTGCCCAGG AGCTGCAGCA AGAGAAAGCA
    ACTACCATGG ATTTGACTAA ACGTTTGGAA AGCACTGTGA AGACTTGTAT AGGTACCAGA
    ATGAAAAATC TGGCAGCTAA GGTTGAAATA TTGAAAGAAA TTAGACGTGT CAGTATCAGT
    GTTCGTTTTG GAAATGACCT TTCAGATGCT ATACAAGTGT TGGATGAAGA CTGCTTTACT
    ATCCCAGCTT CTTTGAATGA ATTAGAGCTA ATTTGGGCAG AATATAATCT GGCTCAGGAG
    AAGATTCAAA CTTGTGAGAA TGTGAATGAA GGGAATATTT TGATTGCTGA AAGAAATGAA
    GTACAACAGA AGCTGTACAT GGCAGTAGAA GTTTTTATTC TGGAAGTTGA TGAGTTATCT
    CTTAATAAAC GCTTAAAAAC GTTGCAGGAT TTGTCAGCCT CTTTAGAAGC AGTATATGGA
    CAGGCCAAAG AAGGAATTAA CTCTGAAGAA ATACTGGAGA AATTTAATGA CTGGAGGTGT
    AATAAAAGAG AGGAGTTCAC CAGTGTTAGA AGTGAAACAG AGACTTGTAT ACAACGTCTT
    ATAGCATGGT TCCAGAATAC TTTAAAGGCC TTTGATCTGT CTGTGGACAA ATCACTGATT
    TCTGATGACA CAATTGATAA TATTGATGAA ATCCTGGAGA ACACTGAGTC AGGTGTCTGC
    AAGGAGCTGG AGATATCTCT GATTGAACAA GGTGATGCAG ACAAGGAGAT CATTTTAAAA
    ACATGTAGTC AAGTACTACA AAAGATCCGT TCAGAGGAAA GCCTTATTGC CACAGTACAA
    TCCAAGTACA AGGACAGTGT TGAGTTTAAG AAACAGATTA TTGAATGTTT AAATAAGAGC
    CCCAGCGTGG ATCACTTGCT TTCCATTAAA AAGACACTGA AAAGCTTAAA GGCTCGACTG
    AGATGGAAAT TGGTTGAAAA GAATAATTTG GAAGAATCTG ATGACCATGA TGGATCTGAG
    ATTGAAAAAA TAAAACAAGA AATAACTCAG TTACGGAATA GTGTCTTTGA GGGAATTTAT
    CATGAGCAGG AGGAATATGC GAAGCTGAGT AGCTTAGTAC AAAAATGGTT CCCTGAGCTT
    CCTCTACTTC ATCCTGAAAT AGGATTACTT AAGTACATGA ACTCTGGTGG TCTTCTTACA
    ATGAGCTTGG AAAGAGATCT TCTTGATGCT GAGCCTATGA AGGAGCTTAG CAGCAAGCGT
    CCTTTGATAT GTTCTGAGGT TAATGGGCAG ATAATTCTTT TAAAGGGCTA TTCTGTGGAT
    GTCGACACAG AAGCCAGGGT AATTCAGAGA GCAGCTGCCT ATCACACAGC TTGGAGAGAA
    GCTAAAGGAG AATCTGGGTT GCTACCACTG ATATTCTTAT TTTTGTGTAA GTCGGATCCT
    ATGGCTTATC TGATGGTCCC ATATTATCCT AGGGCAAATC TGAATGCTGT TCAGTCCACC
    ATGCCTTTAA CTTCAGAAGA AGTTTTAAAG GTCATGAAAG GTGTAGCCCA GGGCCTTCAT
    ACATTGCACA AGGCTGACAT AGTTCATGGA TCACTTCATC AGAACAATGT TTTTGCTTTA
    AACCGTCAAC ATGGAATTGT TGGAGATTTT GACTTCACCA AATCTGTGAG TCAGCGAACC
    TCAGTGAACA TGATGGTTGG TGACTTAAGT TTGCTATCAC CTGAACTGAA AATGGGAAAA
    CCTGCTTCTC CGAGTTCTGA CTTATATGCT TATGGCTGCC TTTTATTGTG GCTTTCAGTT
    CAGAATCAGG AATTTGAAAC TAATGAGGAT GGAATCCCAA AGATGGATCA GTTTCATTTG
    GATGATAATG TCAAATCCCT CCTCTGTAGC TTGATCTGTT GTAGAAGTTC AATGACTGCC
    GAGCAGGTTT TGAATGCTGA CTGTTTCTTG CTACCAAAGG GAAAATCAAT GCCAAACTCA
    GAGAAAGATA CTGAATACAT CCCCAAAAAA GAGGAAGAAG AATTAAAGGT GAAGAACTTG
    GATAAAGATA AGGAAAAGAC AAGAAATGGT GAAGCCAGCT TTGATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010802645.1
    CDS1325..4312
    Translation

    Target ORF information:

    RefSeq Version XM_010804343.2
    Organism Bos taurus(cattle)
    Definition Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010804343.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGAGGAAG ATAAACATTG CAATAAAGTT GAAGATGTGG TTGGAAGTCA TGTGGAAGAT 
    GCAGTAACAT TTTGGGCCCA GAGTATCAAT AGAAATAAGG ATATTATGAA GATTGGTTGT
    TCACTGTCTG AAGTCTGTCC CCTTGCCAAT TCAGTGTTTG GGAATCTTGA TCCAAAGAAG
    ATTTATGGTG GATTATTTTC TGAAGATAAA TGTTGGTACA GATGCCAAGT ACTGAAAGTC
    ATCAACGATG AAAAGTGTCT GGTGAGATAT ATTGACTATG GAAATACAGA AATTCTGAAT
    CGATCGGATA TAGTAGAGAT TCCTTTGGAT CTGCAATTTT CTAGTGTTGC CAAAAAGTAT
    AGACTTTGGG GACTACAGAT TCCTTCTAGC CAAGAAGTTA CCCAGTTTGA TCAGGGCAGA
    ACCTTTTTGG GGAGCTTGAT TTTTGAAAAG GAAATTAAAA TGAGAATTAA AGCTACCTAT
    CAAGATGGAA CAATAATTGC TCAGGCTGAG TATGGCAATG TAGATATTGG GGAAGAGGTG
    GCTAAGAAAG GATTTGCAGA AAAATGCAGA CTCACTTCCA GCACTAATAT CTGTGAGGAA
    AAAAAATCAG ATCCTGGTCA GCTTGTCCTC AGGAACCTCA AAAGCCCCAT TCCTTTGTGG
    GGGCATAGAT CAAGCCAATC AAACTTCACC CGGCCAAAGG GGCGCTTAAG TGGGAGAATG
    TGTCTTGACT TGAAGAATGA AAATGATGCA GGAAATAATG TGACATTTCC AAAGGAAAAT
    TTGGCTGTTG GTGACTTTAA TTTAGGGTCT AATGTCAGCC TGGCAAAAAT TAAGCAGGAC
    CAGAAACTGA TTGAAGAAAA TGAAAAACTT AAAACAGAGA AGGAGGTTCT TCTTGAAAGG
    TGCAAGGCAT TAGAATTGAA AGTCGAACAG ACTGCCCAGG AGCTGCAGCA AGAGAAAGCA
    ACTACCATGG ATTTGACTAA ACGTTTGGAA AGCACTGTGA AGACTTGTAT AGGTACCAGA
    ATGAAAAATC TGGCAGCTAA GGTTGAAATA TTGAAAGAAA TTAGACGTGT CAGTATCAGT
    GTTCGTTTTG GAAATGACCT TTCAGATGCT ATACAAGTGT TGGATGAAGA CTGCTTTACT
    ATCCCAGCTT CTTTGAATGA ATTAGAGCTA ATTTGGGCAG AATATAATCT GGCTCAGGAG
    AAGATTCAAA CTTGTGAGAA TGTGAATGAA GGGAATATTT TGATTGCTGA AAGAAATGAA
    GTACAACAGA AGCTGTACAT GGCAGTAGAA GTTTTTATTC TGGAAGTTGA TGAGTTATCT
    CTTAATAAAC GCTTAAAAAC GTTGCAGGAT TTGTCAGCCT CTTTAGAAGC AGTATATGGA
    CAGGCCAAAG AAGGAATTAA CTCTGAAGAA ATACTGGAGA AATTTAATGA CTGGAGGTGT
    AATAAAAGAG AGGAGTTCAC CAGTGTTAGA AGTGAAACAG AGACTTGTAT ACAACGTCTT
    ATAGCATGGT TCCAGAATAC TTTAAAGGCC TTTGATCTGT CTGTGGACAA ATCACTGATT
    TCTGATGACA CAATTGATAA TATTGATGAA ATCCTGGAGA ACACTGAGTC AGGTGTCTGC
    AAGGAGCTGG AGATATCTCT GATTGAACAA GGTGATGCAG ACAAGGAGAT CATTTTAAAA
    ACATGTAGTC AAGTACTACA AAAGATCCGT TCAGAGGAAA GCCTTATTGC CACAGTACAA
    TCCAAGTACA AGGACAGTGT TGAGTTTAAG AAACAGATTA TTGAATGTTT AAATAAGAGC
    CCCAGCGTGG ATCACTTGCT TTCCATTAAA AAGACACTGA AAAGCTTAAA GGCTCGACTG
    AGATGGAAAT TGGTTGAAAA GAATAATTTG GAAGAATCTG ATGACCATGA TGGATCTGAG
    ATTGAAAAAA TAAAACAAGA AATAACTCAG TTACGGAATA GTGTCTTTGA GGGAATTTAT
    CATGAGCAGG AGGAATATGC GAAGCTGAGT AGCTTAGTAC AAAAATGGTT CCCTGAGCTT
    CCTCTACTTC ATCCTGAAAT AGGATTACTT AAGTACATGA ACTCTGGTGG TCTTCTTACA
    ATGAGCTTGG AAAGAGATCT TCTTGATGCT GAGCCTATGA AGGAGCTTAG CAGCAAGCGT
    CCTTTGATAT GTTCTGAGGT TAATGGGCAG ATAATTCTTT TAAAGGGCTA TTCTGTGGAT
    GTCGACACAG AAGCCAGGGT AATTCAGAGA GCAGCTGCCT ATCACACAGC TTGGAGAGAA
    GCTAAAGGAG AATCTGGGTT GCTACCACTG ATATTCTTAT TTTTGTGTAA GTCGGATCCT
    ATGGCTTATC TGATGGTCCC ATATTATCCT AGGGCAAATC TGAATGCTGT TCAGTCCACC
    ATGCCTTTAA CTTCAGAAGA AGTTTTAAAG GTCATGAAAG GTGTAGCCCA GGGCCTTCAT
    ACATTGCACA AGGCTGACAT AGTTCATGGA TCACTTCATC AGAACAATGT TTTTGCTTTA
    AACCGTCAAC ATGGAATTGT TGGAGATTTT GACTTCACCA AATCTGTGAG TCAGCGAACC
    TCAGTGAACA TGATGGTTGG TGACTTAAGT TTGCTATCAC CTGAACTGAA AATGGGAAAA
    CCTGCTTCTC CGAGTTCTGA CTTATATGCT TATGGCTGCC TTTTATTGTG GCTTTCAGTT
    CAGAATCAGG AATTTGAAAC TAATGAGGAT GGAATCCCAA AGATGGATCA GTTTCATTTG
    GATGATAATG TCAAATCCCT CCTCTGTAGC TTGATCTGTT GTAGAAGTTC AATGACTGCC
    GAGCAGGTTT TGAATGCTGA CTGTTTCTTG CTACCAAAGG GAAAATCAAT GCCAAACTCA
    GAGAAAGATA CTGAATACAT CCCCAAAAAA GAGGAAGAAG AATTAAAGGT GAAGAACTTG
    GATAAAGATA AGGAAAAGAC AAGAAATGGT GAAGCCAGCT TTGATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18657
    Clone ID Related Accession (Same CDS sequence) XM_015463070.1 , XM_015469733.1 , XM_015469733.2 , XM_010804343.2 , XM_024990537.1
    Accession Version XM_024990537.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2988bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein FAM221A isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGAGGAAG ATAAACATTG CAATAAAGTT GAAGATGTGG TTGGAAGTCA TGTGGAAGAT 
    GCAGTAACAT TTTGGGCCCA GAGTATCAAT AGAAATAAGG ATATTATGAA GATTGGTTGT
    TCACTGTCTG AAGTCTGTCC CCTTGCCAAT TCAGTGTTTG GGAATCTTGA TCCAAAGAAG
    ATTTATGGTG GATTATTTTC TGAAGATAAA TGTTGGTACA GATGCCAAGT ACTGAAAGTC
    ATCAACGATG AAAAGTGTCT GGTGAGATAT ATTGACTATG GAAATACAGA AATTCTGAAT
    CGATCGGATA TAGTAGAGAT TCCTTTGGAT CTGCAATTTT CTAGTGTTGC CAAAAAGTAT
    AGACTTTGGG GACTACAGAT TCCTTCTAGC CAAGAAGTTA CCCAGTTTGA TCAGGGCAGA
    ACCTTTTTGG GGAGCTTGAT TTTTGAAAAG GAAATTAAAA TGAGAATTAA AGCTACCTAT
    CAAGATGGAA CAATAATTGC TCAGGCTGAG TATGGCAATG TAGATATTGG GGAAGAGGTG
    GCTAAGAAAG GATTTGCAGA AAAATGCAGA CTCACTTCCA GCACTAATAT CTGTGAGGAA
    AAAAAATCAG ATCCTGGTCA GCTTGTCCTC AGGAACCTCA AAAGCCCCAT TCCTTTGTGG
    GGGCATAGAT CAAGCCAATC AAACTTCACC CGGCCAAAGG GGCGCTTAAG TGGGAGAATG
    TGTCTTGACT TGAAGAATGA AAATGATGCA GGAAATAATG TGACATTTCC AAAGGAAAAT
    TTGGCTGTTG GTGACTTTAA TTTAGGGTCT AATGTCAGCC TGGCAAAAAT TAAGCAGGAC
    CAGAAACTGA TTGAAGAAAA TGAAAAACTT AAAACAGAGA AGGAGGTTCT TCTTGAAAGG
    TGCAAGGCAT TAGAATTGAA AGTCGAACAG ACTGCCCAGG AGCTGCAGCA AGAGAAAGCA
    ACTACCATGG ATTTGACTAA ACGTTTGGAA AGCACTGTGA AGACTTGTAT AGGTACCAGA
    ATGAAAAATC TGGCAGCTAA GGTTGAAATA TTGAAAGAAA TTAGACGTGT CAGTATCAGT
    GTTCGTTTTG GAAATGACCT TTCAGATGCT ATACAAGTGT TGGATGAAGA CTGCTTTACT
    ATCCCAGCTT CTTTGAATGA ATTAGAGCTA ATTTGGGCAG AATATAATCT GGCTCAGGAG
    AAGATTCAAA CTTGTGAGAA TGTGAATGAA GGGAATATTT TGATTGCTGA AAGAAATGAA
    GTACAACAGA AGCTGTACAT GGCAGTAGAA GTTTTTATTC TGGAAGTTGA TGAGTTATCT
    CTTAATAAAC GCTTAAAAAC GTTGCAGGAT TTGTCAGCCT CTTTAGAAGC AGTATATGGA
    CAGGCCAAAG AAGGAATTAA CTCTGAAGAA ATACTGGAGA AATTTAATGA CTGGAGGTGT
    AATAAAAGAG AGGAGTTCAC CAGTGTTAGA AGTGAAACAG AGACTTGTAT ACAACGTCTT
    ATAGCATGGT TCCAGAATAC TTTAAAGGCC TTTGATCTGT CTGTGGACAA ATCACTGATT
    TCTGATGACA CAATTGATAA TATTGATGAA ATCCTGGAGA ACACTGAGTC AGGTGTCTGC
    AAGGAGCTGG AGATATCTCT GATTGAACAA GGTGATGCAG ACAAGGAGAT CATTTTAAAA
    ACATGTAGTC AAGTACTACA AAAGATCCGT TCAGAGGAAA GCCTTATTGC CACAGTACAA
    TCCAAGTACA AGGACAGTGT TGAGTTTAAG AAACAGATTA TTGAATGTTT AAATAAGAGC
    CCCAGCGTGG ATCACTTGCT TTCCATTAAA AAGACACTGA AAAGCTTAAA GGCTCGACTG
    AGATGGAAAT TGGTTGAAAA GAATAATTTG GAAGAATCTG ATGACCATGA TGGATCTGAG
    ATTGAAAAAA TAAAACAAGA AATAACTCAG TTACGGAATA GTGTCTTTGA GGGAATTTAT
    CATGAGCAGG AGGAATATGC GAAGCTGAGT AGCTTAGTAC AAAAATGGTT CCCTGAGCTT
    CCTCTACTTC ATCCTGAAAT AGGATTACTT AAGTACATGA ACTCTGGTGG TCTTCTTACA
    ATGAGCTTGG AAAGAGATCT TCTTGATGCT GAGCCTATGA AGGAGCTTAG CAGCAAGCGT
    CCTTTGATAT GTTCTGAGGT TAATGGGCAG ATAATTCTTT TAAAGGGCTA TTCTGTGGAT
    GTCGACACAG AAGCCAGGGT AATTCAGAGA GCAGCTGCCT ATCACACAGC TTGGAGAGAA
    GCTAAAGGAG AATCTGGGTT GCTACCACTG ATATTCTTAT TTTTGTGTAA GTCGGATCCT
    ATGGCTTATC TGATGGTCCC ATATTATCCT AGGGCAAATC TGAATGCTGT TCAGTCCACC
    ATGCCTTTAA CTTCAGAAGA AGTTTTAAAG GTCATGAAAG GTGTAGCCCA GGGCCTTCAT
    ACATTGCACA AGGCTGACAT AGTTCATGGA TCACTTCATC AGAACAATGT TTTTGCTTTA
    AACCGTCAAC ATGGAATTGT TGGAGATTTT GACTTCACCA AATCTGTGAG TCAGCGAACC
    TCAGTGAACA TGATGGTTGG TGACTTAAGT TTGCTATCAC CTGAACTGAA AATGGGAAAA
    CCTGCTTCTC CGAGTTCTGA CTTATATGCT TATGGCTGCC TTTTATTGTG GCTTTCAGTT
    CAGAATCAGG AATTTGAAAC TAATGAGGAT GGAATCCCAA AGATGGATCA GTTTCATTTG
    GATGATAATG TCAAATCCCT CCTCTGTAGC TTGATCTGTT GTAGAAGTTC AATGACTGCC
    GAGCAGGTTT TGAATGCTGA CTGTTTCTTG CTACCAAAGG GAAAATCAAT GCCAAACTCA
    GAGAAAGATA CTGAATACAT CCCCAAAAAA GAGGAAGAAG AATTAAAGGT GAAGAACTTG
    GATAAAGATA AGGAAAAGAC AAGAAATGGT GAAGCCAGCT TTGATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024846305.1
    CDS229..3216
    Translation

    Target ORF information:

    RefSeq Version XM_024990537.1
    Organism Bos taurus(cattle)
    Definition Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024990537.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGAGGAAG ATAAACATTG CAATAAAGTT GAAGATGTGG TTGGAAGTCA TGTGGAAGAT 
    GCAGTAACAT TTTGGGCCCA GAGTATCAAT AGAAATAAGG ATATTATGAA GATTGGTTGT
    TCACTGTCTG AAGTCTGTCC CCTTGCCAAT TCAGTGTTTG GGAATCTTGA TCCAAAGAAG
    ATTTATGGTG GATTATTTTC TGAAGATAAA TGTTGGTACA GATGCCAAGT ACTGAAAGTC
    ATCAACGATG AAAAGTGTCT GGTGAGATAT ATTGACTATG GAAATACAGA AATTCTGAAT
    CGATCGGATA TAGTAGAGAT TCCTTTGGAT CTGCAATTTT CTAGTGTTGC CAAAAAGTAT
    AGACTTTGGG GACTACAGAT TCCTTCTAGC CAAGAAGTTA CCCAGTTTGA TCAGGGCAGA
    ACCTTTTTGG GGAGCTTGAT TTTTGAAAAG GAAATTAAAA TGAGAATTAA AGCTACCTAT
    CAAGATGGAA CAATAATTGC TCAGGCTGAG TATGGCAATG TAGATATTGG GGAAGAGGTG
    GCTAAGAAAG GATTTGCAGA AAAATGCAGA CTCACTTCCA GCACTAATAT CTGTGAGGAA
    AAAAAATCAG ATCCTGGTCA GCTTGTCCTC AGGAACCTCA AAAGCCCCAT TCCTTTGTGG
    GGGCATAGAT CAAGCCAATC AAACTTCACC CGGCCAAAGG GGCGCTTAAG TGGGAGAATG
    TGTCTTGACT TGAAGAATGA AAATGATGCA GGAAATAATG TGACATTTCC AAAGGAAAAT
    TTGGCTGTTG GTGACTTTAA TTTAGGGTCT AATGTCAGCC TGGCAAAAAT TAAGCAGGAC
    CAGAAACTGA TTGAAGAAAA TGAAAAACTT AAAACAGAGA AGGAGGTTCT TCTTGAAAGG
    TGCAAGGCAT TAGAATTGAA AGTCGAACAG ACTGCCCAGG AGCTGCAGCA AGAGAAAGCA
    ACTACCATGG ATTTGACTAA ACGTTTGGAA AGCACTGTGA AGACTTGTAT AGGTACCAGA
    ATGAAAAATC TGGCAGCTAA GGTTGAAATA TTGAAAGAAA TTAGACGTGT CAGTATCAGT
    GTTCGTTTTG GAAATGACCT TTCAGATGCT ATACAAGTGT TGGATGAAGA CTGCTTTACT
    ATCCCAGCTT CTTTGAATGA ATTAGAGCTA ATTTGGGCAG AATATAATCT GGCTCAGGAG
    AAGATTCAAA CTTGTGAGAA TGTGAATGAA GGGAATATTT TGATTGCTGA AAGAAATGAA
    GTACAACAGA AGCTGTACAT GGCAGTAGAA GTTTTTATTC TGGAAGTTGA TGAGTTATCT
    CTTAATAAAC GCTTAAAAAC GTTGCAGGAT TTGTCAGCCT CTTTAGAAGC AGTATATGGA
    CAGGCCAAAG AAGGAATTAA CTCTGAAGAA ATACTGGAGA AATTTAATGA CTGGAGGTGT
    AATAAAAGAG AGGAGTTCAC CAGTGTTAGA AGTGAAACAG AGACTTGTAT ACAACGTCTT
    ATAGCATGGT TCCAGAATAC TTTAAAGGCC TTTGATCTGT CTGTGGACAA ATCACTGATT
    TCTGATGACA CAATTGATAA TATTGATGAA ATCCTGGAGA ACACTGAGTC AGGTGTCTGC
    AAGGAGCTGG AGATATCTCT GATTGAACAA GGTGATGCAG ACAAGGAGAT CATTTTAAAA
    ACATGTAGTC AAGTACTACA AAAGATCCGT TCAGAGGAAA GCCTTATTGC CACAGTACAA
    TCCAAGTACA AGGACAGTGT TGAGTTTAAG AAACAGATTA TTGAATGTTT AAATAAGAGC
    CCCAGCGTGG ATCACTTGCT TTCCATTAAA AAGACACTGA AAAGCTTAAA GGCTCGACTG
    AGATGGAAAT TGGTTGAAAA GAATAATTTG GAAGAATCTG ATGACCATGA TGGATCTGAG
    ATTGAAAAAA TAAAACAAGA AATAACTCAG TTACGGAATA GTGTCTTTGA GGGAATTTAT
    CATGAGCAGG AGGAATATGC GAAGCTGAGT AGCTTAGTAC AAAAATGGTT CCCTGAGCTT
    CCTCTACTTC ATCCTGAAAT AGGATTACTT AAGTACATGA ACTCTGGTGG TCTTCTTACA
    ATGAGCTTGG AAAGAGATCT TCTTGATGCT GAGCCTATGA AGGAGCTTAG CAGCAAGCGT
    CCTTTGATAT GTTCTGAGGT TAATGGGCAG ATAATTCTTT TAAAGGGCTA TTCTGTGGAT
    GTCGACACAG AAGCCAGGGT AATTCAGAGA GCAGCTGCCT ATCACACAGC TTGGAGAGAA
    GCTAAAGGAG AATCTGGGTT GCTACCACTG ATATTCTTAT TTTTGTGTAA GTCGGATCCT
    ATGGCTTATC TGATGGTCCC ATATTATCCT AGGGCAAATC TGAATGCTGT TCAGTCCACC
    ATGCCTTTAA CTTCAGAAGA AGTTTTAAAG GTCATGAAAG GTGTAGCCCA GGGCCTTCAT
    ACATTGCACA AGGCTGACAT AGTTCATGGA TCACTTCATC AGAACAATGT TTTTGCTTTA
    AACCGTCAAC ATGGAATTGT TGGAGATTTT GACTTCACCA AATCTGTGAG TCAGCGAACC
    TCAGTGAACA TGATGGTTGG TGACTTAAGT TTGCTATCAC CTGAACTGAA AATGGGAAAA
    CCTGCTTCTC CGAGTTCTGA CTTATATGCT TATGGCTGCC TTTTATTGTG GCTTTCAGTT
    CAGAATCAGG AATTTGAAAC TAATGAGGAT GGAATCCCAA AGATGGATCA GTTTCATTTG
    GATGATAATG TCAAATCCCT CCTCTGTAGC TTGATCTGTT GTAGAAGTTC AATGACTGCC
    GAGCAGGTTT TGAATGCTGA CTGTTTCTTG CTACCAAAGG GAAAATCAAT GCCAAACTCA
    GAGAAAGATA CTGAATACAT CCCCAAAAAA GAGGAAGAAG AATTAAAGGT GAAGAACTTG
    GATAAAGATA AGGAAAAGAC AAGAAATGGT GAAGCCAGCT TTGATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa233579
    Clone ID Related Accession (Same CDS sequence) XM_024990534.1
    Accession Version XM_024990534.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3687bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein FAM221A isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGAGCGGC TGACGTTACC TCCTGGCGGC GCGGCGGCAG TGGACGAGTA CTTGGAGTAC 
    CGGAGAATTG TTGGTGAGGA TGACGGAGGG AAACTTTTTA CTCCTGAGGA ATATGAAGAA
    TACAAAAGAA AAGTCTTACC TATGCGTTTA CAGAACAGAT TGTATGTGAG TTGGCAGTCA
    CCCACGGGAA TGGATTGTAA ACTTGTGGGT CCAGAGACAC TGTGTTTTTG TACACATAGG
    TATAAGCAAC ATAAAACTGA CTTTGAAACG ATTCCCCCGC AGCGCCCCAT CAGTCTGCCT
    TGCCAAGTGA GGGGCTGCCC TTGCCGGGCT TACGTCTACG TGCCTCTGAA CGGCACACAG
    CCCATTCGCT GCAGATGTTC TAAGTGTTCG GGATTTCATA GTTGCTTTAC TTGTGCTTGT
    GGTCAGCCTG CATATGCCCA TGACACAGTA GTGGAAACTA AGCAAGAAAG ACTGGCTCAG
    GGAAAACCAG TGGGGCAGGA CGTTCCTTAT GCAGCAATGG GAGGATTAAC TGGCTTCAGC
    TCGCTGGCAG AAGGCTACAT ACGATTAGAT GACAGTGGAA TTGGTGCACC ATCAGCTGAA
    TTTTTAGACT CTCCAGTTAT GGCCATGGAC CACCCATTTC TAAAGGCATT TCAAGCATCA
    TCTAGCTCTT CTCCAGAAAC GCTAACAGGA ATTGTCCAAA TGGAGGAAGA TAAACATTGC
    AATAAAGTTG AAGATGTGGT TGGAAGTCAT GTGGAAGATG CAGTAACATT TTGGGCCCAG
    AGTATCAATA GAAATAAGGA TATTATGAAG ATTGGTTGTT CACTGTCTGA AGTCTGTCCC
    CTTGCCAATT CAGTGTTTGG GAATCTTGAT CCAAAGAAGA TTTATGGTGG ATTATTTTCT
    GAAGATAAAT GTTGGTACAG ATGCCAAGTA CTGAAAGTCA TCAACGATGA AAAGTGTCTG
    GTGAGATATA TTGACTATGG AAATACAGAA ATTCTGAATC GATCGGATAT AGTAGAGATT
    CCTTTGGATC TGCAATTTTC TAGTGTTGCC AAAAAGTATA GACTTTGGGG ACTACAGATT
    CCTTCTAGCC AAGAAGTTAC CCAGTTTGAT CAGGGCAGAA CCTTTTTGGG GAGCTTGATT
    TTTGAAAAGG AAATTAAAAT GAGAATTAAA GCTACCTATC AAGATGGAAC AATAATTGCT
    CAGGCTGAGT ATGGCAATGT AGATATTGGG GAAGAGGTGG CTAAGAAAGG ATTTGCAGAA
    AAATGCAGAC TCACTTCCAG CACTAATATC TGTGAGGAAA AAAAATCAGA TCCTGGTCAG
    CTTGTCCTCA GGAACCTCAA AAGCCCCATT CCTTTGTGGG GGCATAGATC AAGCCAATCA
    AACTTCACCC GGCCAAAGGG GCGCTTAAGT GGGAGAATGT GTCTTGACTT GAAGAATGAA
    AATGATGCAG GAAATAATGT GACATTTCCA AAGGAAAATT TGGCTGTTGG TGACTTTAAT
    TTAGGGTCTA ATGTCAGCCT GGCAAAAATT AAGCAGGACC AGAAACTGAT TGAAGAAAAT
    GAAAAACTTA AAACAGAGAA GGAGGTTCTT CTTGAAAGGT GCAAGGCATT AGAATTGAAA
    GTCGAACAGA CTGCCCAGGA GCTGCAGCAA GAGAAAGCAA CTACCATGGA TTTGACTAAA
    CGTTTGGAAA GCACTGTGAA GACTTGTATA GGTACCAGAA TGAAAAATCT GGCAGCTAAG
    GTTGAAATAT TGAAAGAAAT TAGACGTGTC AGTATCAGTG TTCGTTTTGG AAATGACCTT
    TCAGATGCTA TACAAGTGTT GGATGAAGAC TGCTTTACTA TCCCAGCTTC TTTGAATGAA
    TTAGAGCTAA TTTGGGCAGA ATATAATCTG GCTCAGGAGA AGATTCAAAC TTGTGAGAAT
    GTGAATGAAG GGAATATTTT GATTGCTGAA AGAAATGAAG TACAACAGAA GCTGTACATG
    GCAGTAGAAG TTTTTATTCT GGAAGTTGAT GAGTTATCTC TTAATAAACG CTTAAAAACG
    TTGCAGGATT TGTCAGCCTC TTTAGAAGCA GTATATGGAC AGGCCAAAGA AGGAATTAAC
    TCTGAAGAAA TACTGGAGAA ATTTAATGAC TGGAGGTGTA ATAAAAGAGA GGAGTTCACC
    AGTGTTAGAA GTGAAACAGA GACTTGTATA CAACGTCTTA TAGCATGGTT CCAGAATACT
    TTAAAGGCCT TTGATCTGTC TGTGGACAAA TCACTGATTT CTGATGACAC AATTGATAAT
    ATTGATGAAA TCCTGGAGAA CACTGAGTCA GGTGTCTGCA AGGAGCTGGA GATATCTCTG
    ATTGAACAAG GTGATGCAGA CAAGGAGATC ATTTTAAAAA CATGTAGTCA AGTACTACAA
    AAGATCCGTT CAGAGGAAAG CCTTATTGCC ACAGTACAAT CCAAGTACAA GGACAGTGTT
    GAGTTTAAGA AACAGATTAT TGAATGTTTA AATAAGAGCC CCAGCGTGGA TCACTTGCTT
    TCCATTAAAA AGACACTGAA AAGCTTAAAG GCTCGACTGA GATGGAAATT GGTTGAAAAG
    AATAATTTGG AAGAATCTGA TGACCATGAT GGATCTGAGA TTGAAAAAAT AAAACAAGAA
    ATAACTCAGT TACGGAATAG TGTCTTTGAG GGAATTTATC ATGAGCAGGA GGAATATGCG
    AAGCTGAGTA GCTTAGTACA AAAATGGTTC CCTGAGCTTC CTCTACTTCA TCCTGAAATA
    GGATTACTTA AGTACATGAA CTCTGGTGGT CTTCTTACAA TGAGCTTGGA AAGAGATCTT
    CTTGATGCTG AGCCTATGAA GGAGCTTAGC AGCAAGCGTC CTTTGATATG TTCTGAGGTT
    AATGGGCAGA TAATTCTTTT AAAGGGCTAT TCTGTGGATG TCGACACAGA AGCCAGGGTA
    ATTCAGAGAG CAGCTGCCTA TCACACAGCT TGGAGAGAAG CTAAAGGAGA ATCTGGGTTG
    CTACCACTGA TATTCTTATT TTTGTGTAAG TCGGATCCTA TGGCTTATCT GATGGTCCCA
    TATTATCCTA GGGCAAATCT GAATGCTGTT CAGTCCACCA TGCCTTTAAC TTCAGAAGAA
    GTTTTAAAGG TCATGAAAGG TGTAGCCCAG GGCCTTCATA CATTGCACAA GGCTGACATA
    GTTCATGGAT CACTTCATCA GAACAATGTT TTTGCTTTAA ACCGTCAACA TGGAATTGTT
    GGAGATTTTG ACTTCACCAA ATCTGTGAGT CAGCGAACCT CAGTGAACAT GATGGTTGGT
    GACTTAAGTT TGCTATCACC TGAACTGAAA ATGGGAAAAC CTGCTTCTCC GAGTTCTGAC
    TTATATGCTT ATGGCTGCCT TTTATTGTGG CTTTCAGTTC AGAATCAGGA ATTTGAAACT
    AATGAGGATG GAATCCCAAA GATGGATCAG TTTCATTTGG ATGATAATGT CAAATCCCTC
    CTCTGTAGCT TGATCTGTTG TAGAAGTTCA ATGACTGCCG AGCAGGTTTT GAATGCTGAC
    TGTTTCTTGC TACCAAAGGG AAAATCAATG CCAAACTCAG AGAAAGATAC TGAATACATC
    CCCAAAAAAG AGGAAGAAGA ATTAAAGGTG AAGAACTTGG ATAAAGATAA GGAAAAGACA
    AGAAATGGTG AAGCCAGCTT TGATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024846302.1
    CDS573..4259
    Translation

    Target ORF information:

    RefSeq Version XM_024990534.1
    Organism Bos taurus(cattle)
    Definition Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024990534.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGAGCGGC TGACGTTACC TCCTGGCGGC GCGGCGGCAG TGGACGAGTA CTTGGAGTAC 
    CGGAGAATTG TTGGTGAGGA TGACGGAGGG AAACTTTTTA CTCCTGAGGA ATATGAAGAA
    TACAAAAGAA AAGTCTTACC TATGCGTTTA CAGAACAGAT TGTATGTGAG TTGGCAGTCA
    CCCACGGGAA TGGATTGTAA ACTTGTGGGT CCAGAGACAC TGTGTTTTTG TACACATAGG
    TATAAGCAAC ATAAAACTGA CTTTGAAACG ATTCCCCCGC AGCGCCCCAT CAGTCTGCCT
    TGCCAAGTGA GGGGCTGCCC TTGCCGGGCT TACGTCTACG TGCCTCTGAA CGGCACACAG
    CCCATTCGCT GCAGATGTTC TAAGTGTTCG GGATTTCATA GTTGCTTTAC TTGTGCTTGT
    GGTCAGCCTG CATATGCCCA TGACACAGTA GTGGAAACTA AGCAAGAAAG ACTGGCTCAG
    GGAAAACCAG TGGGGCAGGA CGTTCCTTAT GCAGCAATGG GAGGATTAAC TGGCTTCAGC
    TCGCTGGCAG AAGGCTACAT ACGATTAGAT GACAGTGGAA TTGGTGCACC ATCAGCTGAA
    TTTTTAGACT CTCCAGTTAT GGCCATGGAC CACCCATTTC TAAAGGCATT TCAAGCATCA
    TCTAGCTCTT CTCCAGAAAC GCTAACAGGA ATTGTCCAAA TGGAGGAAGA TAAACATTGC
    AATAAAGTTG AAGATGTGGT TGGAAGTCAT GTGGAAGATG CAGTAACATT TTGGGCCCAG
    AGTATCAATA GAAATAAGGA TATTATGAAG ATTGGTTGTT CACTGTCTGA AGTCTGTCCC
    CTTGCCAATT CAGTGTTTGG GAATCTTGAT CCAAAGAAGA TTTATGGTGG ATTATTTTCT
    GAAGATAAAT GTTGGTACAG ATGCCAAGTA CTGAAAGTCA TCAACGATGA AAAGTGTCTG
    GTGAGATATA TTGACTATGG AAATACAGAA ATTCTGAATC GATCGGATAT AGTAGAGATT
    CCTTTGGATC TGCAATTTTC TAGTGTTGCC AAAAAGTATA GACTTTGGGG ACTACAGATT
    CCTTCTAGCC AAGAAGTTAC CCAGTTTGAT CAGGGCAGAA CCTTTTTGGG GAGCTTGATT
    TTTGAAAAGG AAATTAAAAT GAGAATTAAA GCTACCTATC AAGATGGAAC AATAATTGCT
    CAGGCTGAGT ATGGCAATGT AGATATTGGG GAAGAGGTGG CTAAGAAAGG ATTTGCAGAA
    AAATGCAGAC TCACTTCCAG CACTAATATC TGTGAGGAAA AAAAATCAGA TCCTGGTCAG
    CTTGTCCTCA GGAACCTCAA AAGCCCCATT CCTTTGTGGG GGCATAGATC AAGCCAATCA
    AACTTCACCC GGCCAAAGGG GCGCTTAAGT GGGAGAATGT GTCTTGACTT GAAGAATGAA
    AATGATGCAG GAAATAATGT GACATTTCCA AAGGAAAATT TGGCTGTTGG TGACTTTAAT
    TTAGGGTCTA ATGTCAGCCT GGCAAAAATT AAGCAGGACC AGAAACTGAT TGAAGAAAAT
    GAAAAACTTA AAACAGAGAA GGAGGTTCTT CTTGAAAGGT GCAAGGCATT AGAATTGAAA
    GTCGAACAGA CTGCCCAGGA GCTGCAGCAA GAGAAAGCAA CTACCATGGA TTTGACTAAA
    CGTTTGGAAA GCACTGTGAA GACTTGTATA GGTACCAGAA TGAAAAATCT GGCAGCTAAG
    GTTGAAATAT TGAAAGAAAT TAGACGTGTC AGTATCAGTG TTCGTTTTGG AAATGACCTT
    TCAGATGCTA TACAAGTGTT GGATGAAGAC TGCTTTACTA TCCCAGCTTC TTTGAATGAA
    TTAGAGCTAA TTTGGGCAGA ATATAATCTG GCTCAGGAGA AGATTCAAAC TTGTGAGAAT
    GTGAATGAAG GGAATATTTT GATTGCTGAA AGAAATGAAG TACAACAGAA GCTGTACATG
    GCAGTAGAAG TTTTTATTCT GGAAGTTGAT GAGTTATCTC TTAATAAACG CTTAAAAACG
    TTGCAGGATT TGTCAGCCTC TTTAGAAGCA GTATATGGAC AGGCCAAAGA AGGAATTAAC
    TCTGAAGAAA TACTGGAGAA ATTTAATGAC TGGAGGTGTA ATAAAAGAGA GGAGTTCACC
    AGTGTTAGAA GTGAAACAGA GACTTGTATA CAACGTCTTA TAGCATGGTT CCAGAATACT
    TTAAAGGCCT TTGATCTGTC TGTGGACAAA TCACTGATTT CTGATGACAC AATTGATAAT
    ATTGATGAAA TCCTGGAGAA CACTGAGTCA GGTGTCTGCA AGGAGCTGGA GATATCTCTG
    ATTGAACAAG GTGATGCAGA CAAGGAGATC ATTTTAAAAA CATGTAGTCA AGTACTACAA
    AAGATCCGTT CAGAGGAAAG CCTTATTGCC ACAGTACAAT CCAAGTACAA GGACAGTGTT
    GAGTTTAAGA AACAGATTAT TGAATGTTTA AATAAGAGCC CCAGCGTGGA TCACTTGCTT
    TCCATTAAAA AGACACTGAA AAGCTTAAAG GCTCGACTGA GATGGAAATT GGTTGAAAAG
    AATAATTTGG AAGAATCTGA TGACCATGAT GGATCTGAGA TTGAAAAAAT AAAACAAGAA
    ATAACTCAGT TACGGAATAG TGTCTTTGAG GGAATTTATC ATGAGCAGGA GGAATATGCG
    AAGCTGAGTA GCTTAGTACA AAAATGGTTC CCTGAGCTTC CTCTACTTCA TCCTGAAATA
    GGATTACTTA AGTACATGAA CTCTGGTGGT CTTCTTACAA TGAGCTTGGA AAGAGATCTT
    CTTGATGCTG AGCCTATGAA GGAGCTTAGC AGCAAGCGTC CTTTGATATG TTCTGAGGTT
    AATGGGCAGA TAATTCTTTT AAAGGGCTAT TCTGTGGATG TCGACACAGA AGCCAGGGTA
    ATTCAGAGAG CAGCTGCCTA TCACACAGCT TGGAGAGAAG CTAAAGGAGA ATCTGGGTTG
    CTACCACTGA TATTCTTATT TTTGTGTAAG TCGGATCCTA TGGCTTATCT GATGGTCCCA
    TATTATCCTA GGGCAAATCT GAATGCTGTT CAGTCCACCA TGCCTTTAAC TTCAGAAGAA
    GTTTTAAAGG TCATGAAAGG TGTAGCCCAG GGCCTTCATA CATTGCACAA GGCTGACATA
    GTTCATGGAT CACTTCATCA GAACAATGTT TTTGCTTTAA ACCGTCAACA TGGAATTGTT
    GGAGATTTTG ACTTCACCAA ATCTGTGAGT CAGCGAACCT CAGTGAACAT GATGGTTGGT
    GACTTAAGTT TGCTATCACC TGAACTGAAA ATGGGAAAAC CTGCTTCTCC GAGTTCTGAC
    TTATATGCTT ATGGCTGCCT TTTATTGTGG CTTTCAGTTC AGAATCAGGA ATTTGAAACT
    AATGAGGATG GAATCCCAAA GATGGATCAG TTTCATTTGG ATGATAATGT CAAATCCCTC
    CTCTGTAGCT TGATCTGTTG TAGAAGTTCA ATGACTGCCG AGCAGGTTTT GAATGCTGAC
    TGTTTCTTGC TACCAAAGGG AAAATCAATG CCAAACTCAG AGAAAGATAC TGAATACATC
    CCCAAAAAAG AGGAAGAAGA ATTAAAGGTG AAGAACTTGG ATAAAGATAA GGAAAAGACA
    AGAAATGGTG AAGCCAGCTT TGATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90622
    Clone ID Related Accession (Same CDS sequence) XM_015469714.1 , XM_015469714.2
    Accession Version XM_015469714.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3741bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein FAM221A isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103900.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGAGCGGC TGACGTTACC TCCTGGCGGC GCGGCGGCAG TGGACGAGTA CTTGGAGTAC 
    CGGAGAATTG TTGGTGAGGA TGACGGAGGG AAACTTTTTA CTCCTGAGGA ATATGAAGAA
    TACAAAAGAA AAGTCTTACC TATGCGTTTA CAGAACAGAT TGTATGTGAG TTGGCAGTCA
    CCCACGGGAA TGGATTGTAA ACTTGTGGGT CCAGAGACAC TGTGTTTTTG TACACATAGG
    TATAAGCAAC ATAAAACTGA CTTTGAAACG ATTCCCCCGC AGCGCCCCAT CAGTCTGCCT
    TGCCAAGTGA GGGGCTGCCC TTGCCGGGCT TACGTCTACG TGCCTCTGAA CGGCACACAG
    CCCATTCGCT GCAGATGTAA GCACTTTGCC GATCAACACA GTGCAGCGCC GGGCTTTGTA
    TGCAATGCCT GTTCTAAGTG TTCGGGATTT CATAGTTGCT TTACTTGTGC TTGTGGTCAG
    CCTGCATATG CCCATGACAC AGTAGTGGAA ACTAAGCAAG AAAGACTGGC TCAGGGAAAA
    CCAGTGGGGC AGGACGTTCC TTATGCAGCA ATGGGAGGAT TAACTGGCTT CAGCTCGCTG
    GCAGAAGGCT ACATACGATT AGATGACAGT GGAATTGGTG CACCATCAGC TGAATTTTTA
    GACTCTCCAG TTATGGCCAT GGACCACCCA TTTCTAAAGG CATTTCAAGC ATCATCTAGC
    TCTTCTCCAG AAACGCTAAC AGGAATTGTC CAAATGGAGG AAGATAAACA TTGCAATAAA
    GTTGAAGATG TGGTTGGAAG TCATGTGGAA GATGCAGTAA CATTTTGGGC CCAGAGTATC
    AATAGAAATA AGGATATTAT GAAGATTGGT TGTTCACTGT CTGAAGTCTG TCCCCTTGCC
    AATTCAGTGT TTGGGAATCT TGATCCAAAG AAGATTTATG GTGGATTATT TTCTGAAGAT
    AAATGTTGGT ACAGATGCCA AGTACTGAAA GTCATCAACG ATGAAAAGTG TCTGGTGAGA
    TATATTGACT ATGGAAATAC AGAAATTCTG AATCGATCGG ATATAGTAGA GATTCCTTTG
    GATCTGCAAT TTTCTAGTGT TGCCAAAAAG TATAGACTTT GGGGACTACA GATTCCTTCT
    AGCCAAGAAG TTACCCAGTT TGATCAGGGC AGAACCTTTT TGGGGAGCTT GATTTTTGAA
    AAGGAAATTA AAATGAGAAT TAAAGCTACC TATCAAGATG GAACAATAAT TGCTCAGGCT
    GAGTATGGCA ATGTAGATAT TGGGGAAGAG GTGGCTAAGA AAGGATTTGC AGAAAAATGC
    AGACTCACTT CCAGCACTAA TATCTGTGAG GAAAAAAAAT CAGATCCTGG TCAGCTTGTC
    CTCAGGAACC TCAAAAGCCC CATTCCTTTG TGGGGGCATA GATCAAGCCA ATCAAACTTC
    ACCCGGCCAA AGGGGCGCTT AAGTGGGAGA ATGTGTCTTG ACTTGAAGAA TGAAAATGAT
    GCAGGAAATA ATGTGACATT TCCAAAGGAA AATTTGGCTG TTGGTGACTT TAATTTAGGG
    TCTAATGTCA GCCTGGCAAA AATTAAGCAG GACCAGAAAC TGATTGAAGA AAATGAAAAA
    CTTAAAACAG AGAAGGAGGT TCTTCTTGAA AGGTGCAAGG CATTAGAATT GAAAGTCGAA
    CAGACTGCCC AGGAGCTGCA GCAAGAGAAA GCAACTACCA TGGATTTGAC TAAACGTTTG
    GAAAGCACTG TGAAGACTTG TATAGGTACC AGAATGAAAA ATCTGGCAGC TAAGGTTGAA
    ATATTGAAAG AAATTAGACG TGTCAGTATC AGTGTTCGTT TTGGAAATGA CCTTTCAGAT
    GCTATACAAG TGTTGGATGA AGACTGCTTT ACTATCCCAG CTTCTTTGAA TGAATTAGAG
    CTAATTTGGG CAGAATATAA TCTGGCTCAG GAGAAGATTC AAACTTGTGA GAATGTGAAT
    GAAGGGAATA TTTTGATTGC TGAAAGAAAT GAAGTACAAC AGAAGCTGTA CATGGCAGTA
    GAAGTTTTTA TTCTGGAAGT TGATGAGTTA TCTCTTAATA AACGCTTAAA AACGTTGCAG
    GATTTGTCAG CCTCTTTAGA AGCAGTATAT GGACAGGCCA AAGAAGGAAT TAACTCTGAA
    GAAATACTGG AGAAATTTAA TGACTGGAGG TGTAATAAAA GAGAGGAGTT CACCAGTGTT
    AGAAGTGAAA CAGAGACTTG TATACAACGT CTTATAGCAT GGTTCCAGAA TACTTTAAAG
    GCCTTTGATC TGTCTGTGGA CAAATCACTG ATTTCTGATG ACACAATTGA TAATATTGAT
    GAAATCCTGG AGAACACTGA GTCAGGTGTC TGCAAGGAGC TGGAGATATC TCTGATTGAA
    CAAGGTGATG CAGACAAGGA GATCATTTTA AAAACATGTA GTCAAGTACT ACAAAAGATC
    CGTTCAGAGG AAAGCCTTAT TGCCACAGTA CAATCCAAGT ACAAGGACAG TGTTGAGTTT
    AAGAAACAGA TTATTGAATG TTTAAATAAG AGCCCCAGCG TGGATCACTT GCTTTCCATT
    AAAAAGACAC TGAAAAGCTT AAAGGCTCGA CTGAGATGGA AATTGGTTGA AAAGAATAAT
    TTGGAAGAAT CTGATGACCA TGATGGATCT GAGATTGAAA AAATAAAACA AGAAATAACT
    CAGTTACGGA ATAGTGTCTT TGAGGGAATT TATCATGAGC AGGAGGAATA TGCGAAGCTG
    AGTAGCTTAG TACAAAAATG GTTCCCTGAG CTTCCTCTAC TTCATCCTGA AATAGGATTA
    CTTAAGTACA TGAACTCTGG TGGTCTTCTT ACAATGAGCT TGGAAAGAGA TCTTCTTGAT
    GCTGAGCCTA TGAAGGAGCT TAGCAGCAAG CGTCCTTTGA TATGTTCTGA GGTTAATGGG
    CAGATAATTC TTTTAAAGGG CTATTCTGTG GATGTCGACA CAGAAGCCAG GGTAATTCAG
    AGAGCAGCTG CCTATCACAC AGCTTGGAGA GAAGCTAAAG GAGAATCTGG GTTGCTACCA
    CTGATATTCT TATTTTTGTG TAAGTCGGAT CCTATGGCTT ATCTGATGGT CCCATATTAT
    CCTAGGGCAA ATCTGAATGC TGTTCAGTCC ACCATGCCTT TAACTTCAGA AGAAGTTTTA
    AAGGTCATGA AAGGTGTAGC CCAGGGCCTT CATACATTGC ACAAGGCTGA CATAGTTCAT
    GGATCACTTC ATCAGAACAA TGTTTTTGCT TTAAACCGTC AACATGGAAT TGTTGGAGAT
    TTTGACTTCA CCAAATCTGT GAGTCAGCGA ACCTCAGTGA ACATGATGGT TGGTGACTTA
    AGTTTGCTAT CACCTGAACT GAAAATGGGA AAACCTGCTT CTCCGAGTTC TGACTTATAT
    GCTTATGGCT GCCTTTTATT GTGGCTTTCA GTTCAGAATC AGGAATTTGA AACTAATGAG
    GATGGAATCC CAAAGATGGA TCAGTTTCAT TTGGATGATA ATGTCAAATC CCTCCTCTGT
    AGCTTGATCT GTTGTAGAAG TTCAATGACT GCCGAGCAGG TTTTGAATGC TGACTGTTTC
    TTGCTACCAA AGGGAAAATC AATGCCAAAC TCAGAGAAAG ATACTGAATA CATCCCCAAA
    AAAGAGGAAG AAGAATTAAA GGTGAAGAAC TTGGATAAAG ATAAGGAAAA GACAAGAAAT
    GGTGAAGCCA GCTTTGATTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015325200.1
    CDS80..3820
    Translation

    Target ORF information:

    RefSeq Version XM_015469714.1
    Organism Bos taurus(cattle)
    Definition Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015469714.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGAGCGGC TGACGTTACC TCCTGGCGGC GCGGCGGCAG TGGACGAGTA CTTGGAGTAC 
    CGGAGAATTG TTGGTGAGGA TGACGGAGGG AAACTTTTTA CTCCTGAGGA ATATGAAGAA
    TACAAAAGAA AAGTCTTACC TATGCGTTTA CAGAACAGAT TGTATGTGAG TTGGCAGTCA
    CCCACGGGAA TGGATTGTAA ACTTGTGGGT CCAGAGACAC TGTGTTTTTG TACACATAGG
    TATAAGCAAC ATAAAACTGA CTTTGAAACG ATTCCCCCGC AGCGCCCCAT CAGTCTGCCT
    TGCCAAGTGA GGGGCTGCCC TTGCCGGGCT TACGTCTACG TGCCTCTGAA CGGCACACAG
    CCCATTCGCT GCAGATGTAA GCACTTTGCC GATCAACACA GTGCAGCGCC GGGCTTTGTA
    TGCAATGCCT GTTCTAAGTG TTCGGGATTT CATAGTTGCT TTACTTGTGC TTGTGGTCAG
    CCTGCATATG CCCATGACAC AGTAGTGGAA ACTAAGCAAG AAAGACTGGC TCAGGGAAAA
    CCAGTGGGGC AGGACGTTCC TTATGCAGCA ATGGGAGGAT TAACTGGCTT CAGCTCGCTG
    GCAGAAGGCT ACATACGATT AGATGACAGT GGAATTGGTG CACCATCAGC TGAATTTTTA
    GACTCTCCAG TTATGGCCAT GGACCACCCA TTTCTAAAGG CATTTCAAGC ATCATCTAGC
    TCTTCTCCAG AAACGCTAAC AGGAATTGTC CAAATGGAGG AAGATAAACA TTGCAATAAA
    GTTGAAGATG TGGTTGGAAG TCATGTGGAA GATGCAGTAA CATTTTGGGC CCAGAGTATC
    AATAGAAATA AGGATATTAT GAAGATTGGT TGTTCACTGT CTGAAGTCTG TCCCCTTGCC
    AATTCAGTGT TTGGGAATCT TGATCCAAAG AAGATTTATG GTGGATTATT TTCTGAAGAT
    AAATGTTGGT ACAGATGCCA AGTACTGAAA GTCATCAACG ATGAAAAGTG TCTGGTGAGA
    TATATTGACT ATGGAAATAC AGAAATTCTG AATCGATCGG ATATAGTAGA GATTCCTTTG
    GATCTGCAAT TTTCTAGTGT TGCCAAAAAG TATAGACTTT GGGGACTACA GATTCCTTCT
    AGCCAAGAAG TTACCCAGTT TGATCAGGGC AGAACCTTTT TGGGGAGCTT GATTTTTGAA
    AAGGAAATTA AAATGAGAAT TAAAGCTACC TATCAAGATG GAACAATAAT TGCTCAGGCT
    GAGTATGGCA ATGTAGATAT TGGGGAAGAG GTGGCTAAGA AAGGATTTGC AGAAAAATGC
    AGACTCACTT CCAGCACTAA TATCTGTGAG GAAAAAAAAT CAGATCCTGG TCAGCTTGTC
    CTCAGGAACC TCAAAAGCCC CATTCCTTTG TGGGGGCATA GATCAAGCCA ATCAAACTTC
    ACCCGGCCAA AGGGGCGCTT AAGTGGGAGA ATGTGTCTTG ACTTGAAGAA TGAAAATGAT
    GCAGGAAATA ATGTGACATT TCCAAAGGAA AATTTGGCTG TTGGTGACTT TAATTTAGGG
    TCTAATGTCA GCCTGGCAAA AATTAAGCAG GACCAGAAAC TGATTGAAGA AAATGAAAAA
    CTTAAAACAG AGAAGGAGGT TCTTCTTGAA AGGTGCAAGG CATTAGAATT GAAAGTCGAA
    CAGACTGCCC AGGAGCTGCA GCAAGAGAAA GCAACTACCA TGGATTTGAC TAAACGTTTG
    GAAAGCACTG TGAAGACTTG TATAGGTACC AGAATGAAAA ATCTGGCAGC TAAGGTTGAA
    ATATTGAAAG AAATTAGACG TGTCAGTATC AGTGTTCGTT TTGGAAATGA CCTTTCAGAT
    GCTATACAAG TGTTGGATGA AGACTGCTTT ACTATCCCAG CTTCTTTGAA TGAATTAGAG
    CTAATTTGGG CAGAATATAA TCTGGCTCAG GAGAAGATTC AAACTTGTGA GAATGTGAAT
    GAAGGGAATA TTTTGATTGC TGAAAGAAAT GAAGTACAAC AGAAGCTGTA CATGGCAGTA
    GAAGTTTTTA TTCTGGAAGT TGATGAGTTA TCTCTTAATA AACGCTTAAA AACGTTGCAG
    GATTTGTCAG CCTCTTTAGA AGCAGTATAT GGACAGGCCA AAGAAGGAAT TAACTCTGAA
    GAAATACTGG AGAAATTTAA TGACTGGAGG TGTAATAAAA GAGAGGAGTT CACCAGTGTT
    AGAAGTGAAA CAGAGACTTG TATACAACGT CTTATAGCAT GGTTCCAGAA TACTTTAAAG
    GCCTTTGATC TGTCTGTGGA CAAATCACTG ATTTCTGATG ACACAATTGA TAATATTGAT
    GAAATCCTGG AGAACACTGA GTCAGGTGTC TGCAAGGAGC TGGAGATATC TCTGATTGAA
    CAAGGTGATG CAGACAAGGA GATCATTTTA AAAACATGTA GTCAAGTACT ACAAAAGATC
    CGTTCAGAGG AAAGCCTTAT TGCCACAGTA CAATCCAAGT ACAAGGACAG TGTTGAGTTT
    AAGAAACAGA TTATTGAATG TTTAAATAAG AGCCCCAGCG TGGATCACTT GCTTTCCATT
    AAAAAGACAC TGAAAAGCTT AAAGGCTCGA CTGAGATGGA AATTGGTTGA AAAGAATAAT
    TTGGAAGAAT CTGATGACCA TGATGGATCT GAGATTGAAA AAATAAAACA AGAAATAACT
    CAGTTACGGA ATAGTGTCTT TGAGGGAATT TATCATGAGC AGGAGGAATA TGCGAAGCTG
    AGTAGCTTAG TACAAAAATG GTTCCCTGAG CTTCCTCTAC TTCATCCTGA AATAGGATTA
    CTTAAGTACA TGAACTCTGG TGGTCTTCTT ACAATGAGCT TGGAAAGAGA TCTTCTTGAT
    GCTGAGCCTA TGAAGGAGCT TAGCAGCAAG CGTCCTTTGA TATGTTCTGA GGTTAATGGG
    CAGATAATTC TTTTAAAGGG CTATTCTGTG GATGTCGACA CAGAAGCCAG GGTAATTCAG
    AGAGCAGCTG CCTATCACAC AGCTTGGAGA GAAGCTAAAG GAGAATCTGG GTTGCTACCA
    CTGATATTCT TATTTTTGTG TAAGTCGGAT CCTATGGCTT ATCTGATGGT CCCATATTAT
    CCTAGGGCAA ATCTGAATGC TGTTCAGTCC ACCATGCCTT TAACTTCAGA AGAAGTTTTA
    AAGGTCATGA AAGGTGTAGC CCAGGGCCTT CATACATTGC ACAAGGCTGA CATAGTTCAT
    GGATCACTTC ATCAGAACAA TGTTTTTGCT TTAAACCGTC AACATGGAAT TGTTGGAGAT
    TTTGACTTCA CCAAATCTGT GAGTCAGCGA ACCTCAGTGA ACATGATGGT TGGTGACTTA
    AGTTTGCTAT CACCTGAACT GAAAATGGGA AAACCTGCTT CTCCGAGTTC TGACTTATAT
    GCTTATGGCT GCCTTTTATT GTGGCTTTCA GTTCAGAATC AGGAATTTGA AACTAATGAG
    GATGGAATCC CAAAGATGGA TCAGTTTCAT TTGGATGATA ATGTCAAATC CCTCCTCTGT
    AGCTTGATCT GTTGTAGAAG TTCAATGACT GCCGAGCAGG TTTTGAATGC TGACTGTTTC
    TTGCTACCAA AGGGAAAATC AATGCCAAAC TCAGAGAAAG ATACTGAATA CATCCCCAAA
    AAAGAGGAAG AAGAATTAAA GGTGAAGAAC TTGGATAAAG ATAAGGAAAA GACAAGAAAT
    GGTGAAGCCA GCTTTGATTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90622
    Clone ID Related Accession (Same CDS sequence) XM_015469714.1 , XM_015469714.2
    Accession Version XM_015469714.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3741bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein FAM221A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015469714.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGAGCGGC TGACGTTACC TCCTGGCGGC GCGGCGGCAG TGGACGAGTA CTTGGAGTAC 
    CGGAGAATTG TTGGTGAGGA TGACGGAGGG AAACTTTTTA CTCCTGAGGA ATATGAAGAA
    TACAAAAGAA AAGTCTTACC TATGCGTTTA CAGAACAGAT TGTATGTGAG TTGGCAGTCA
    CCCACGGGAA TGGATTGTAA ACTTGTGGGT CCAGAGACAC TGTGTTTTTG TACACATAGG
    TATAAGCAAC ATAAAACTGA CTTTGAAACG ATTCCCCCGC AGCGCCCCAT CAGTCTGCCT
    TGCCAAGTGA GGGGCTGCCC TTGCCGGGCT TACGTCTACG TGCCTCTGAA CGGCACACAG
    CCCATTCGCT GCAGATGTAA GCACTTTGCC GATCAACACA GTGCAGCGCC GGGCTTTGTA
    TGCAATGCCT GTTCTAAGTG TTCGGGATTT CATAGTTGCT TTACTTGTGC TTGTGGTCAG
    CCTGCATATG CCCATGACAC AGTAGTGGAA ACTAAGCAAG AAAGACTGGC TCAGGGAAAA
    CCAGTGGGGC AGGACGTTCC TTATGCAGCA ATGGGAGGAT TAACTGGCTT CAGCTCGCTG
    GCAGAAGGCT ACATACGATT AGATGACAGT GGAATTGGTG CACCATCAGC TGAATTTTTA
    GACTCTCCAG TTATGGCCAT GGACCACCCA TTTCTAAAGG CATTTCAAGC ATCATCTAGC
    TCTTCTCCAG AAACGCTAAC AGGAATTGTC CAAATGGAGG AAGATAAACA TTGCAATAAA
    GTTGAAGATG TGGTTGGAAG TCATGTGGAA GATGCAGTAA CATTTTGGGC CCAGAGTATC
    AATAGAAATA AGGATATTAT GAAGATTGGT TGTTCACTGT CTGAAGTCTG TCCCCTTGCC
    AATTCAGTGT TTGGGAATCT TGATCCAAAG AAGATTTATG GTGGATTATT TTCTGAAGAT
    AAATGTTGGT ACAGATGCCA AGTACTGAAA GTCATCAACG ATGAAAAGTG TCTGGTGAGA
    TATATTGACT ATGGAAATAC AGAAATTCTG AATCGATCGG ATATAGTAGA GATTCCTTTG
    GATCTGCAAT TTTCTAGTGT TGCCAAAAAG TATAGACTTT GGGGACTACA GATTCCTTCT
    AGCCAAGAAG TTACCCAGTT TGATCAGGGC AGAACCTTTT TGGGGAGCTT GATTTTTGAA
    AAGGAAATTA AAATGAGAAT TAAAGCTACC TATCAAGATG GAACAATAAT TGCTCAGGCT
    GAGTATGGCA ATGTAGATAT TGGGGAAGAG GTGGCTAAGA AAGGATTTGC AGAAAAATGC
    AGACTCACTT CCAGCACTAA TATCTGTGAG GAAAAAAAAT CAGATCCTGG TCAGCTTGTC
    CTCAGGAACC TCAAAAGCCC CATTCCTTTG TGGGGGCATA GATCAAGCCA ATCAAACTTC
    ACCCGGCCAA AGGGGCGCTT AAGTGGGAGA ATGTGTCTTG ACTTGAAGAA TGAAAATGAT
    GCAGGAAATA ATGTGACATT TCCAAAGGAA AATTTGGCTG TTGGTGACTT TAATTTAGGG
    TCTAATGTCA GCCTGGCAAA AATTAAGCAG GACCAGAAAC TGATTGAAGA AAATGAAAAA
    CTTAAAACAG AGAAGGAGGT TCTTCTTGAA AGGTGCAAGG CATTAGAATT GAAAGTCGAA
    CAGACTGCCC AGGAGCTGCA GCAAGAGAAA GCAACTACCA TGGATTTGAC TAAACGTTTG
    GAAAGCACTG TGAAGACTTG TATAGGTACC AGAATGAAAA ATCTGGCAGC TAAGGTTGAA
    ATATTGAAAG AAATTAGACG TGTCAGTATC AGTGTTCGTT TTGGAAATGA CCTTTCAGAT
    GCTATACAAG TGTTGGATGA AGACTGCTTT ACTATCCCAG CTTCTTTGAA TGAATTAGAG
    CTAATTTGGG CAGAATATAA TCTGGCTCAG GAGAAGATTC AAACTTGTGA GAATGTGAAT
    GAAGGGAATA TTTTGATTGC TGAAAGAAAT GAAGTACAAC AGAAGCTGTA CATGGCAGTA
    GAAGTTTTTA TTCTGGAAGT TGATGAGTTA TCTCTTAATA AACGCTTAAA AACGTTGCAG
    GATTTGTCAG CCTCTTTAGA AGCAGTATAT GGACAGGCCA AAGAAGGAAT TAACTCTGAA
    GAAATACTGG AGAAATTTAA TGACTGGAGG TGTAATAAAA GAGAGGAGTT CACCAGTGTT
    AGAAGTGAAA CAGAGACTTG TATACAACGT CTTATAGCAT GGTTCCAGAA TACTTTAAAG
    GCCTTTGATC TGTCTGTGGA CAAATCACTG ATTTCTGATG ACACAATTGA TAATATTGAT
    GAAATCCTGG AGAACACTGA GTCAGGTGTC TGCAAGGAGC TGGAGATATC TCTGATTGAA
    CAAGGTGATG CAGACAAGGA GATCATTTTA AAAACATGTA GTCAAGTACT ACAAAAGATC
    CGTTCAGAGG AAAGCCTTAT TGCCACAGTA CAATCCAAGT ACAAGGACAG TGTTGAGTTT
    AAGAAACAGA TTATTGAATG TTTAAATAAG AGCCCCAGCG TGGATCACTT GCTTTCCATT
    AAAAAGACAC TGAAAAGCTT AAAGGCTCGA CTGAGATGGA AATTGGTTGA AAAGAATAAT
    TTGGAAGAAT CTGATGACCA TGATGGATCT GAGATTGAAA AAATAAAACA AGAAATAACT
    CAGTTACGGA ATAGTGTCTT TGAGGGAATT TATCATGAGC AGGAGGAATA TGCGAAGCTG
    AGTAGCTTAG TACAAAAATG GTTCCCTGAG CTTCCTCTAC TTCATCCTGA AATAGGATTA
    CTTAAGTACA TGAACTCTGG TGGTCTTCTT ACAATGAGCT TGGAAAGAGA TCTTCTTGAT
    GCTGAGCCTA TGAAGGAGCT TAGCAGCAAG CGTCCTTTGA TATGTTCTGA GGTTAATGGG
    CAGATAATTC TTTTAAAGGG CTATTCTGTG GATGTCGACA CAGAAGCCAG GGTAATTCAG
    AGAGCAGCTG CCTATCACAC AGCTTGGAGA GAAGCTAAAG GAGAATCTGG GTTGCTACCA
    CTGATATTCT TATTTTTGTG TAAGTCGGAT CCTATGGCTT ATCTGATGGT CCCATATTAT
    CCTAGGGCAA ATCTGAATGC TGTTCAGTCC ACCATGCCTT TAACTTCAGA AGAAGTTTTA
    AAGGTCATGA AAGGTGTAGC CCAGGGCCTT CATACATTGC ACAAGGCTGA CATAGTTCAT
    GGATCACTTC ATCAGAACAA TGTTTTTGCT TTAAACCGTC AACATGGAAT TGTTGGAGAT
    TTTGACTTCA CCAAATCTGT GAGTCAGCGA ACCTCAGTGA ACATGATGGT TGGTGACTTA
    AGTTTGCTAT CACCTGAACT GAAAATGGGA AAACCTGCTT CTCCGAGTTC TGACTTATAT
    GCTTATGGCT GCCTTTTATT GTGGCTTTCA GTTCAGAATC AGGAATTTGA AACTAATGAG
    GATGGAATCC CAAAGATGGA TCAGTTTCAT TTGGATGATA ATGTCAAATC CCTCCTCTGT
    AGCTTGATCT GTTGTAGAAG TTCAATGACT GCCGAGCAGG TTTTGAATGC TGACTGTTTC
    TTGCTACCAA AGGGAAAATC AATGCCAAAC TCAGAGAAAG ATACTGAATA CATCCCCAAA
    AAAGAGGAAG AAGAATTAAA GGTGAAGAAC TTGGATAAAG ATAAGGAAAA GACAAGAAAT
    GGTGAAGCCA GCTTTGATTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015325200.1
    CDS573..4313
    Translation

    Target ORF information:

    RefSeq Version XM_015469714.2
    Organism Bos taurus(cattle)
    Definition Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015469714.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGAGCGGC TGACGTTACC TCCTGGCGGC GCGGCGGCAG TGGACGAGTA CTTGGAGTAC 
    CGGAGAATTG TTGGTGAGGA TGACGGAGGG AAACTTTTTA CTCCTGAGGA ATATGAAGAA
    TACAAAAGAA AAGTCTTACC TATGCGTTTA CAGAACAGAT TGTATGTGAG TTGGCAGTCA
    CCCACGGGAA TGGATTGTAA ACTTGTGGGT CCAGAGACAC TGTGTTTTTG TACACATAGG
    TATAAGCAAC ATAAAACTGA CTTTGAAACG ATTCCCCCGC AGCGCCCCAT CAGTCTGCCT
    TGCCAAGTGA GGGGCTGCCC TTGCCGGGCT TACGTCTACG TGCCTCTGAA CGGCACACAG
    CCCATTCGCT GCAGATGTAA GCACTTTGCC GATCAACACA GTGCAGCGCC GGGCTTTGTA
    TGCAATGCCT GTTCTAAGTG TTCGGGATTT CATAGTTGCT TTACTTGTGC TTGTGGTCAG
    CCTGCATATG CCCATGACAC AGTAGTGGAA ACTAAGCAAG AAAGACTGGC TCAGGGAAAA
    CCAGTGGGGC AGGACGTTCC TTATGCAGCA ATGGGAGGAT TAACTGGCTT CAGCTCGCTG
    GCAGAAGGCT ACATACGATT AGATGACAGT GGAATTGGTG CACCATCAGC TGAATTTTTA
    GACTCTCCAG TTATGGCCAT GGACCACCCA TTTCTAAAGG CATTTCAAGC ATCATCTAGC
    TCTTCTCCAG AAACGCTAAC AGGAATTGTC CAAATGGAGG AAGATAAACA TTGCAATAAA
    GTTGAAGATG TGGTTGGAAG TCATGTGGAA GATGCAGTAA CATTTTGGGC CCAGAGTATC
    AATAGAAATA AGGATATTAT GAAGATTGGT TGTTCACTGT CTGAAGTCTG TCCCCTTGCC
    AATTCAGTGT TTGGGAATCT TGATCCAAAG AAGATTTATG GTGGATTATT TTCTGAAGAT
    AAATGTTGGT ACAGATGCCA AGTACTGAAA GTCATCAACG ATGAAAAGTG TCTGGTGAGA
    TATATTGACT ATGGAAATAC AGAAATTCTG AATCGATCGG ATATAGTAGA GATTCCTTTG
    GATCTGCAAT TTTCTAGTGT TGCCAAAAAG TATAGACTTT GGGGACTACA GATTCCTTCT
    AGCCAAGAAG TTACCCAGTT TGATCAGGGC AGAACCTTTT TGGGGAGCTT GATTTTTGAA
    AAGGAAATTA AAATGAGAAT TAAAGCTACC TATCAAGATG GAACAATAAT TGCTCAGGCT
    GAGTATGGCA ATGTAGATAT TGGGGAAGAG GTGGCTAAGA AAGGATTTGC AGAAAAATGC
    AGACTCACTT CCAGCACTAA TATCTGTGAG GAAAAAAAAT CAGATCCTGG TCAGCTTGTC
    CTCAGGAACC TCAAAAGCCC CATTCCTTTG TGGGGGCATA GATCAAGCCA ATCAAACTTC
    ACCCGGCCAA AGGGGCGCTT AAGTGGGAGA ATGTGTCTTG ACTTGAAGAA TGAAAATGAT
    GCAGGAAATA ATGTGACATT TCCAAAGGAA AATTTGGCTG TTGGTGACTT TAATTTAGGG
    TCTAATGTCA GCCTGGCAAA AATTAAGCAG GACCAGAAAC TGATTGAAGA AAATGAAAAA
    CTTAAAACAG AGAAGGAGGT TCTTCTTGAA AGGTGCAAGG CATTAGAATT GAAAGTCGAA
    CAGACTGCCC AGGAGCTGCA GCAAGAGAAA GCAACTACCA TGGATTTGAC TAAACGTTTG
    GAAAGCACTG TGAAGACTTG TATAGGTACC AGAATGAAAA ATCTGGCAGC TAAGGTTGAA
    ATATTGAAAG AAATTAGACG TGTCAGTATC AGTGTTCGTT TTGGAAATGA CCTTTCAGAT
    GCTATACAAG TGTTGGATGA AGACTGCTTT ACTATCCCAG CTTCTTTGAA TGAATTAGAG
    CTAATTTGGG CAGAATATAA TCTGGCTCAG GAGAAGATTC AAACTTGTGA GAATGTGAAT
    GAAGGGAATA TTTTGATTGC TGAAAGAAAT GAAGTACAAC AGAAGCTGTA CATGGCAGTA
    GAAGTTTTTA TTCTGGAAGT TGATGAGTTA TCTCTTAATA AACGCTTAAA AACGTTGCAG
    GATTTGTCAG CCTCTTTAGA AGCAGTATAT GGACAGGCCA AAGAAGGAAT TAACTCTGAA
    GAAATACTGG AGAAATTTAA TGACTGGAGG TGTAATAAAA GAGAGGAGTT CACCAGTGTT
    AGAAGTGAAA CAGAGACTTG TATACAACGT CTTATAGCAT GGTTCCAGAA TACTTTAAAG
    GCCTTTGATC TGTCTGTGGA CAAATCACTG ATTTCTGATG ACACAATTGA TAATATTGAT
    GAAATCCTGG AGAACACTGA GTCAGGTGTC TGCAAGGAGC TGGAGATATC TCTGATTGAA
    CAAGGTGATG CAGACAAGGA GATCATTTTA AAAACATGTA GTCAAGTACT ACAAAAGATC
    CGTTCAGAGG AAAGCCTTAT TGCCACAGTA CAATCCAAGT ACAAGGACAG TGTTGAGTTT
    AAGAAACAGA TTATTGAATG TTTAAATAAG AGCCCCAGCG TGGATCACTT GCTTTCCATT
    AAAAAGACAC TGAAAAGCTT AAAGGCTCGA CTGAGATGGA AATTGGTTGA AAAGAATAAT
    TTGGAAGAAT CTGATGACCA TGATGGATCT GAGATTGAAA AAATAAAACA AGAAATAACT
    CAGTTACGGA ATAGTGTCTT TGAGGGAATT TATCATGAGC AGGAGGAATA TGCGAAGCTG
    AGTAGCTTAG TACAAAAATG GTTCCCTGAG CTTCCTCTAC TTCATCCTGA AATAGGATTA
    CTTAAGTACA TGAACTCTGG TGGTCTTCTT ACAATGAGCT TGGAAAGAGA TCTTCTTGAT
    GCTGAGCCTA TGAAGGAGCT TAGCAGCAAG CGTCCTTTGA TATGTTCTGA GGTTAATGGG
    CAGATAATTC TTTTAAAGGG CTATTCTGTG GATGTCGACA CAGAAGCCAG GGTAATTCAG
    AGAGCAGCTG CCTATCACAC AGCTTGGAGA GAAGCTAAAG GAGAATCTGG GTTGCTACCA
    CTGATATTCT TATTTTTGTG TAAGTCGGAT CCTATGGCTT ATCTGATGGT CCCATATTAT
    CCTAGGGCAA ATCTGAATGC TGTTCAGTCC ACCATGCCTT TAACTTCAGA AGAAGTTTTA
    AAGGTCATGA AAGGTGTAGC CCAGGGCCTT CATACATTGC ACAAGGCTGA CATAGTTCAT
    GGATCACTTC ATCAGAACAA TGTTTTTGCT TTAAACCGTC AACATGGAAT TGTTGGAGAT
    TTTGACTTCA CCAAATCTGT GAGTCAGCGA ACCTCAGTGA ACATGATGGT TGGTGACTTA
    AGTTTGCTAT CACCTGAACT GAAAATGGGA AAACCTGCTT CTCCGAGTTC TGACTTATAT
    GCTTATGGCT GCCTTTTATT GTGGCTTTCA GTTCAGAATC AGGAATTTGA AACTAATGAG
    GATGGAATCC CAAAGATGGA TCAGTTTCAT TTGGATGATA ATGTCAAATC CCTCCTCTGT
    AGCTTGATCT GTTGTAGAAG TTCAATGACT GCCGAGCAGG TTTTGAATGC TGACTGTTTC
    TTGCTACCAA AGGGAAAATC AATGCCAAAC TCAGAGAAAG ATACTGAATA CATCCCCAAA
    AAAGAGGAAG AAGAATTAAA GGTGAAGAAC TTGGATAAAG ATAAGGAAAA GACAAGAAAT
    GGTGAAGCCA GCTTTGATTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90623
    Clone ID Related Accession (Same CDS sequence) XM_015469715.2 , XM_015469715.1
    Accession Version XM_015469715.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3618bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein FAM221A isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015469715.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGAGCGGC TGACGTTACC TCCTGGCGGC GCGGCGGCAG TGGACGAGTA CTTGGAGTAC 
    CGGAGAATTG TTGGTGAGGA TGACGGAGGG AAACTTTTTA CTCCTGAGGA ATATGAAGAA
    TACAAAAGAA AAGTCTTACC TATGCGTTTA CAGAACAGAT TGTATGTGAG TTGGCAGTCA
    CCCACGGGAA TGGATTGTAA ACTTGTGGGT CCAGAGACAC TGTGTTTTTG TACACATAGG
    TATAAGCAAC ATAAAACTGA CTTTGAAACG ATTCCCCCGC AGCGCCCCAT CAGTCTGCCT
    TGCCAAGTGA GGGGCTGCCC TTGCCGGGCT TACGTCTACG TGCCTCTGAA CGGCACACAG
    CCCATTCGCT GCAGATGTAA GCACTTTGCC GATCAACACA GTGCAGCGCC GGGCTTTGTA
    TGCAATGCCT GTTCTAAGTG TTCGGGATTT CATAGTTGCT TTACTTGTGC TTGTGGTCAG
    CCTGCATATG CCCATGACAC AGTAGTGGAA ACTAAGCAAG AAAGACTGGC TCAGGGAAAA
    CCAGTGGGGC AGGACGTTCC TTATGCAGCA ATGGGAGGAT TAACTGGCTT CAGCTCGCTG
    GCAGAAGGCT ACATACGATT AGATGACAGT GGAATTGGTG CACCATCAGC TGAATTTTTA
    GACTCTCCAG TTATGGCCAT GGACCACCCA TTTCTAAAGG CATTTCAAGC ATCATCTAGC
    TCTTCTCCAG AAACGCTAAC AGGAATTGTC CAAATGGAGG AAGATAAACA TTGCAATAAA
    GTTGAAGATG TGGTTGGAAG TCATGTGGAA GATGCAGTAA CATTTTGGGC CCAGAGTATC
    AATAGAAATA AGGATATTAT GAAGATTGGT TGTTCACTGT CTGAAGTCTG TCCCCTTGCC
    AATTCAGTGT TTGGGAATCT TGATCCAAAG AAGATTTATG GTGGATTATT TTCTGAAGAT
    AAATGTTGGT ACAGATGCCA AGTACTGAAA GTCATCAACG ATGAAAAGTG TCTGGTGAGA
    TATATTGACT ATGGAAATAC AGAAATTCTG AATCGATCGG ATATAGTAGA GATTCCTTTG
    GATCTGCAAT TTTCTAGTGT TGCCAAAAAG TATAGACTTT GGGGACTACA GATTCCTTCT
    AGCCAAGAAG TTACCCAGTT TGATCAGGGC AGAACCTTTT TGGGGAGCTT GATTTTTGAA
    AAGGAAATTA AAATGAGAAT TAAAGCTACC TATCAAGATG GAACAATAAT TGCTCAGGCT
    GAGTATGGCA ATGTAGATAT TGGGGAAGAG GTGGCTAAGA AAGGATTTGC AGAAAAATGC
    AGACTCACTT CCAGCACTAA TATCTGTGAG GAAAAAAAAT CAGATCCTGG TCAGCTTGTC
    CTCAGGAACC TCAAAAGCCC CATTCCTTTG TGGGGGCATA GATCAAGCCA ATCAAACTTC
    ACCCGGCCAA AGGGGCGCTT AAGTGGGAGA ATGTGTCTTG ACTTGAAGAA TGAAAATGAT
    GCAGGAAATA ATGTGACATT TCCAAAGGAA AATTTGGCTG TTGGTGACTT TAATTTAGGG
    TCTAATGTCA GCCTGGCAAA AATTAAGCAG GACCAGAAAC TGATTGAAGA AAATGAAAAA
    CTTAAAACAG AGAAGGAGGT TCTTCTTGAA AGGTGCAAGG CATTAGAATT GAAAGTCGAA
    CAGACTGCCC AGGAGCTGCA GCAAGAGAAA GCAACTACCA TGGATTTGAC TAAACGTTTG
    GAAAGCACTG TGAAGACTTG TATAGGTACC AGAATGAAAA ATCTGGCAGC TAAGGTTGAA
    ATATTGAAAG AAATTAGACG TGTCAGTATC AGTGTTCGTT TTGGAAATGA CCTTTCAGAT
    GCTATACAAG TGTTGGATGA AGACTGCTTT ACTATCCCAG CTTCTTTGAA TGAATTAGAG
    CTAATTTGGG CAGAATATAA TCTGGCTCAG GAGAAGATTC AAACTTGTGA GAATGTGGAT
    TTGTCAGCCT CTTTAGAAGC AGTATATGGA CAGGCCAAAG AAGGAATTAA CTCTGAAGAA
    ATACTGGAGA AATTTAATGA CTGGAGGTGT AATAAAAGAG AGGAGTTCAC CAGTGTTAGA
    AGTGAAACAG AGACTTGTAT ACAACGTCTT ATAGCATGGT TCCAGAATAC TTTAAAGGCC
    TTTGATCTGT CTGTGGACAA ATCACTGATT TCTGATGACA CAATTGATAA TATTGATGAA
    ATCCTGGAGA ACACTGAGTC AGGTGTCTGC AAGGAGCTGG AGATATCTCT GATTGAACAA
    GGTGATGCAG ACAAGGAGAT CATTTTAAAA ACATGTAGTC AAGTACTACA AAAGATCCGT
    TCAGAGGAAA GCCTTATTGC CACAGTACAA TCCAAGTACA AGGACAGTGT TGAGTTTAAG
    AAACAGATTA TTGAATGTTT AAATAAGAGC CCCAGCGTGG ATCACTTGCT TTCCATTAAA
    AAGACACTGA AAAGCTTAAA GGCTCGACTG AGATGGAAAT TGGTTGAAAA GAATAATTTG
    GAAGAATCTG ATGACCATGA TGGATCTGAG ATTGAAAAAA TAAAACAAGA AATAACTCAG
    TTACGGAATA GTGTCTTTGA GGGAATTTAT CATGAGCAGG AGGAATATGC GAAGCTGAGT
    AGCTTAGTAC AAAAATGGTT CCCTGAGCTT CCTCTACTTC ATCCTGAAAT AGGATTACTT
    AAGTACATGA ACTCTGGTGG TCTTCTTACA ATGAGCTTGG AAAGAGATCT TCTTGATGCT
    GAGCCTATGA AGGAGCTTAG CAGCAAGCGT CCTTTGATAT GTTCTGAGGT TAATGGGCAG
    ATAATTCTTT TAAAGGGCTA TTCTGTGGAT GTCGACACAG AAGCCAGGGT AATTCAGAGA
    GCAGCTGCCT ATCACACAGC TTGGAGAGAA GCTAAAGGAG AATCTGGGTT GCTACCACTG
    ATATTCTTAT TTTTGTGTAA GTCGGATCCT ATGGCTTATC TGATGGTCCC ATATTATCCT
    AGGGCAAATC TGAATGCTGT TCAGTCCACC ATGCCTTTAA CTTCAGAAGA AGTTTTAAAG
    GTCATGAAAG GTGTAGCCCA GGGCCTTCAT ACATTGCACA AGGCTGACAT AGTTCATGGA
    TCACTTCATC AGAACAATGT TTTTGCTTTA AACCGTCAAC ATGGAATTGT TGGAGATTTT
    GACTTCACCA AATCTGTGAG TCAGCGAACC TCAGTGAACA TGATGGTTGG TGACTTAAGT
    TTGCTATCAC CTGAACTGAA AATGGGAAAA CCTGCTTCTC CGAGTTCTGA CTTATATGCT
    TATGGCTGCC TTTTATTGTG GCTTTCAGTT CAGAATCAGG AATTTGAAAC TAATGAGGAT
    GGAATCCCAA AGATGGATCA GTTTCATTTG GATGATAATG TCAAATCCCT CCTCTGTAGC
    TTGATCTGTT GTAGAAGTTC AATGACTGCC GAGCAGGTTT TGAATGCTGA CTGTTTCTTG
    CTACCAAAGG GAAAATCAAT GCCAAACTCA GAGAAAGATA CTGAATACAT CCCCAAAAAA
    GAGGAAGAAG AATTAAAGGT GAAGAACTTG GATAAAGATA AGGAAAAGAC AAGAAATGGT
    GAAGCCAGCT TTGATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015325201.1
    CDS573..4190
    Translation

    Target ORF information:

    RefSeq Version XM_015469715.2
    Organism Bos taurus(cattle)
    Definition Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015469715.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGAGCGGC TGACGTTACC TCCTGGCGGC GCGGCGGCAG TGGACGAGTA CTTGGAGTAC 
    CGGAGAATTG TTGGTGAGGA TGACGGAGGG AAACTTTTTA CTCCTGAGGA ATATGAAGAA
    TACAAAAGAA AAGTCTTACC TATGCGTTTA CAGAACAGAT TGTATGTGAG TTGGCAGTCA
    CCCACGGGAA TGGATTGTAA ACTTGTGGGT CCAGAGACAC TGTGTTTTTG TACACATAGG
    TATAAGCAAC ATAAAACTGA CTTTGAAACG ATTCCCCCGC AGCGCCCCAT CAGTCTGCCT
    TGCCAAGTGA GGGGCTGCCC TTGCCGGGCT TACGTCTACG TGCCTCTGAA CGGCACACAG
    CCCATTCGCT GCAGATGTAA GCACTTTGCC GATCAACACA GTGCAGCGCC GGGCTTTGTA
    TGCAATGCCT GTTCTAAGTG TTCGGGATTT CATAGTTGCT TTACTTGTGC TTGTGGTCAG
    CCTGCATATG CCCATGACAC AGTAGTGGAA ACTAAGCAAG AAAGACTGGC TCAGGGAAAA
    CCAGTGGGGC AGGACGTTCC TTATGCAGCA ATGGGAGGAT TAACTGGCTT CAGCTCGCTG
    GCAGAAGGCT ACATACGATT AGATGACAGT GGAATTGGTG CACCATCAGC TGAATTTTTA
    GACTCTCCAG TTATGGCCAT GGACCACCCA TTTCTAAAGG CATTTCAAGC ATCATCTAGC
    TCTTCTCCAG AAACGCTAAC AGGAATTGTC CAAATGGAGG AAGATAAACA TTGCAATAAA
    GTTGAAGATG TGGTTGGAAG TCATGTGGAA GATGCAGTAA CATTTTGGGC CCAGAGTATC
    AATAGAAATA AGGATATTAT GAAGATTGGT TGTTCACTGT CTGAAGTCTG TCCCCTTGCC
    AATTCAGTGT TTGGGAATCT TGATCCAAAG AAGATTTATG GTGGATTATT TTCTGAAGAT
    AAATGTTGGT ACAGATGCCA AGTACTGAAA GTCATCAACG ATGAAAAGTG TCTGGTGAGA
    TATATTGACT ATGGAAATAC AGAAATTCTG AATCGATCGG ATATAGTAGA GATTCCTTTG
    GATCTGCAAT TTTCTAGTGT TGCCAAAAAG TATAGACTTT GGGGACTACA GATTCCTTCT
    AGCCAAGAAG TTACCCAGTT TGATCAGGGC AGAACCTTTT TGGGGAGCTT GATTTTTGAA
    AAGGAAATTA AAATGAGAAT TAAAGCTACC TATCAAGATG GAACAATAAT TGCTCAGGCT
    GAGTATGGCA ATGTAGATAT TGGGGAAGAG GTGGCTAAGA AAGGATTTGC AGAAAAATGC
    AGACTCACTT CCAGCACTAA TATCTGTGAG GAAAAAAAAT CAGATCCTGG TCAGCTTGTC
    CTCAGGAACC TCAAAAGCCC CATTCCTTTG TGGGGGCATA GATCAAGCCA ATCAAACTTC
    ACCCGGCCAA AGGGGCGCTT AAGTGGGAGA ATGTGTCTTG ACTTGAAGAA TGAAAATGAT
    GCAGGAAATA ATGTGACATT TCCAAAGGAA AATTTGGCTG TTGGTGACTT TAATTTAGGG
    TCTAATGTCA GCCTGGCAAA AATTAAGCAG GACCAGAAAC TGATTGAAGA AAATGAAAAA
    CTTAAAACAG AGAAGGAGGT TCTTCTTGAA AGGTGCAAGG CATTAGAATT GAAAGTCGAA
    CAGACTGCCC AGGAGCTGCA GCAAGAGAAA GCAACTACCA TGGATTTGAC TAAACGTTTG
    GAAAGCACTG TGAAGACTTG TATAGGTACC AGAATGAAAA ATCTGGCAGC TAAGGTTGAA
    ATATTGAAAG AAATTAGACG TGTCAGTATC AGTGTTCGTT TTGGAAATGA CCTTTCAGAT
    GCTATACAAG TGTTGGATGA AGACTGCTTT ACTATCCCAG CTTCTTTGAA TGAATTAGAG
    CTAATTTGGG CAGAATATAA TCTGGCTCAG GAGAAGATTC AAACTTGTGA GAATGTGGAT
    TTGTCAGCCT CTTTAGAAGC AGTATATGGA CAGGCCAAAG AAGGAATTAA CTCTGAAGAA
    ATACTGGAGA AATTTAATGA CTGGAGGTGT AATAAAAGAG AGGAGTTCAC CAGTGTTAGA
    AGTGAAACAG AGACTTGTAT ACAACGTCTT ATAGCATGGT TCCAGAATAC TTTAAAGGCC
    TTTGATCTGT CTGTGGACAA ATCACTGATT TCTGATGACA CAATTGATAA TATTGATGAA
    ATCCTGGAGA ACACTGAGTC AGGTGTCTGC AAGGAGCTGG AGATATCTCT GATTGAACAA
    GGTGATGCAG ACAAGGAGAT CATTTTAAAA ACATGTAGTC AAGTACTACA AAAGATCCGT
    TCAGAGGAAA GCCTTATTGC CACAGTACAA TCCAAGTACA AGGACAGTGT TGAGTTTAAG
    AAACAGATTA TTGAATGTTT AAATAAGAGC CCCAGCGTGG ATCACTTGCT TTCCATTAAA
    AAGACACTGA AAAGCTTAAA GGCTCGACTG AGATGGAAAT TGGTTGAAAA GAATAATTTG
    GAAGAATCTG ATGACCATGA TGGATCTGAG ATTGAAAAAA TAAAACAAGA AATAACTCAG
    TTACGGAATA GTGTCTTTGA GGGAATTTAT CATGAGCAGG AGGAATATGC GAAGCTGAGT
    AGCTTAGTAC AAAAATGGTT CCCTGAGCTT CCTCTACTTC ATCCTGAAAT AGGATTACTT
    AAGTACATGA ACTCTGGTGG TCTTCTTACA ATGAGCTTGG AAAGAGATCT TCTTGATGCT
    GAGCCTATGA AGGAGCTTAG CAGCAAGCGT CCTTTGATAT GTTCTGAGGT TAATGGGCAG
    ATAATTCTTT TAAAGGGCTA TTCTGTGGAT GTCGACACAG AAGCCAGGGT AATTCAGAGA
    GCAGCTGCCT ATCACACAGC TTGGAGAGAA GCTAAAGGAG AATCTGGGTT GCTACCACTG
    ATATTCTTAT TTTTGTGTAA GTCGGATCCT ATGGCTTATC TGATGGTCCC ATATTATCCT
    AGGGCAAATC TGAATGCTGT TCAGTCCACC ATGCCTTTAA CTTCAGAAGA AGTTTTAAAG
    GTCATGAAAG GTGTAGCCCA GGGCCTTCAT ACATTGCACA AGGCTGACAT AGTTCATGGA
    TCACTTCATC AGAACAATGT TTTTGCTTTA AACCGTCAAC ATGGAATTGT TGGAGATTTT
    GACTTCACCA AATCTGTGAG TCAGCGAACC TCAGTGAACA TGATGGTTGG TGACTTAAGT
    TTGCTATCAC CTGAACTGAA AATGGGAAAA CCTGCTTCTC CGAGTTCTGA CTTATATGCT
    TATGGCTGCC TTTTATTGTG GCTTTCAGTT CAGAATCAGG AATTTGAAAC TAATGAGGAT
    GGAATCCCAA AGATGGATCA GTTTCATTTG GATGATAATG TCAAATCCCT CCTCTGTAGC
    TTGATCTGTT GTAGAAGTTC AATGACTGCC GAGCAGGTTT TGAATGCTGA CTGTTTCTTG
    CTACCAAAGG GAAAATCAAT GCCAAACTCA GAGAAAGATA CTGAATACAT CCCCAAAAAA
    GAGGAAGAAG AATTAAAGGT GAAGAACTTG GATAAAGATA AGGAAAAGAC AAGAAATGGT
    GAAGCCAGCT TTGATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90623
    Clone ID Related Accession (Same CDS sequence) XM_015469715.2 , XM_015469715.1
    Accession Version XM_015469715.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3618bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein FAM221A isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103900.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGAGCGGC TGACGTTACC TCCTGGCGGC GCGGCGGCAG TGGACGAGTA CTTGGAGTAC 
    CGGAGAATTG TTGGTGAGGA TGACGGAGGG AAACTTTTTA CTCCTGAGGA ATATGAAGAA
    TACAAAAGAA AAGTCTTACC TATGCGTTTA CAGAACAGAT TGTATGTGAG TTGGCAGTCA
    CCCACGGGAA TGGATTGTAA ACTTGTGGGT CCAGAGACAC TGTGTTTTTG TACACATAGG
    TATAAGCAAC ATAAAACTGA CTTTGAAACG ATTCCCCCGC AGCGCCCCAT CAGTCTGCCT
    TGCCAAGTGA GGGGCTGCCC TTGCCGGGCT TACGTCTACG TGCCTCTGAA CGGCACACAG
    CCCATTCGCT GCAGATGTAA GCACTTTGCC GATCAACACA GTGCAGCGCC GGGCTTTGTA
    TGCAATGCCT GTTCTAAGTG TTCGGGATTT CATAGTTGCT TTACTTGTGC TTGTGGTCAG
    CCTGCATATG CCCATGACAC AGTAGTGGAA ACTAAGCAAG AAAGACTGGC TCAGGGAAAA
    CCAGTGGGGC AGGACGTTCC TTATGCAGCA ATGGGAGGAT TAACTGGCTT CAGCTCGCTG
    GCAGAAGGCT ACATACGATT AGATGACAGT GGAATTGGTG CACCATCAGC TGAATTTTTA
    GACTCTCCAG TTATGGCCAT GGACCACCCA TTTCTAAAGG CATTTCAAGC ATCATCTAGC
    TCTTCTCCAG AAACGCTAAC AGGAATTGTC CAAATGGAGG AAGATAAACA TTGCAATAAA
    GTTGAAGATG TGGTTGGAAG TCATGTGGAA GATGCAGTAA CATTTTGGGC CCAGAGTATC
    AATAGAAATA AGGATATTAT GAAGATTGGT TGTTCACTGT CTGAAGTCTG TCCCCTTGCC
    AATTCAGTGT TTGGGAATCT TGATCCAAAG AAGATTTATG GTGGATTATT TTCTGAAGAT
    AAATGTTGGT ACAGATGCCA AGTACTGAAA GTCATCAACG ATGAAAAGTG TCTGGTGAGA
    TATATTGACT ATGGAAATAC AGAAATTCTG AATCGATCGG ATATAGTAGA GATTCCTTTG
    GATCTGCAAT TTTCTAGTGT TGCCAAAAAG TATAGACTTT GGGGACTACA GATTCCTTCT
    AGCCAAGAAG TTACCCAGTT TGATCAGGGC AGAACCTTTT TGGGGAGCTT GATTTTTGAA
    AAGGAAATTA AAATGAGAAT TAAAGCTACC TATCAAGATG GAACAATAAT TGCTCAGGCT
    GAGTATGGCA ATGTAGATAT TGGGGAAGAG GTGGCTAAGA AAGGATTTGC AGAAAAATGC
    AGACTCACTT CCAGCACTAA TATCTGTGAG GAAAAAAAAT CAGATCCTGG TCAGCTTGTC
    CTCAGGAACC TCAAAAGCCC CATTCCTTTG TGGGGGCATA GATCAAGCCA ATCAAACTTC
    ACCCGGCCAA AGGGGCGCTT AAGTGGGAGA ATGTGTCTTG ACTTGAAGAA TGAAAATGAT
    GCAGGAAATA ATGTGACATT TCCAAAGGAA AATTTGGCTG TTGGTGACTT TAATTTAGGG
    TCTAATGTCA GCCTGGCAAA AATTAAGCAG GACCAGAAAC TGATTGAAGA AAATGAAAAA
    CTTAAAACAG AGAAGGAGGT TCTTCTTGAA AGGTGCAAGG CATTAGAATT GAAAGTCGAA
    CAGACTGCCC AGGAGCTGCA GCAAGAGAAA GCAACTACCA TGGATTTGAC TAAACGTTTG
    GAAAGCACTG TGAAGACTTG TATAGGTACC AGAATGAAAA ATCTGGCAGC TAAGGTTGAA
    ATATTGAAAG AAATTAGACG TGTCAGTATC AGTGTTCGTT TTGGAAATGA CCTTTCAGAT
    GCTATACAAG TGTTGGATGA AGACTGCTTT ACTATCCCAG CTTCTTTGAA TGAATTAGAG
    CTAATTTGGG CAGAATATAA TCTGGCTCAG GAGAAGATTC AAACTTGTGA GAATGTGGAT
    TTGTCAGCCT CTTTAGAAGC AGTATATGGA CAGGCCAAAG AAGGAATTAA CTCTGAAGAA
    ATACTGGAGA AATTTAATGA CTGGAGGTGT AATAAAAGAG AGGAGTTCAC CAGTGTTAGA
    AGTGAAACAG AGACTTGTAT ACAACGTCTT ATAGCATGGT TCCAGAATAC TTTAAAGGCC
    TTTGATCTGT CTGTGGACAA ATCACTGATT TCTGATGACA CAATTGATAA TATTGATGAA
    ATCCTGGAGA ACACTGAGTC AGGTGTCTGC AAGGAGCTGG AGATATCTCT GATTGAACAA
    GGTGATGCAG ACAAGGAGAT CATTTTAAAA ACATGTAGTC AAGTACTACA AAAGATCCGT
    TCAGAGGAAA GCCTTATTGC CACAGTACAA TCCAAGTACA AGGACAGTGT TGAGTTTAAG
    AAACAGATTA TTGAATGTTT AAATAAGAGC CCCAGCGTGG ATCACTTGCT TTCCATTAAA
    AAGACACTGA AAAGCTTAAA GGCTCGACTG AGATGGAAAT TGGTTGAAAA GAATAATTTG
    GAAGAATCTG ATGACCATGA TGGATCTGAG ATTGAAAAAA TAAAACAAGA AATAACTCAG
    TTACGGAATA GTGTCTTTGA GGGAATTTAT CATGAGCAGG AGGAATATGC GAAGCTGAGT
    AGCTTAGTAC AAAAATGGTT CCCTGAGCTT CCTCTACTTC ATCCTGAAAT AGGATTACTT
    AAGTACATGA ACTCTGGTGG TCTTCTTACA ATGAGCTTGG AAAGAGATCT TCTTGATGCT
    GAGCCTATGA AGGAGCTTAG CAGCAAGCGT CCTTTGATAT GTTCTGAGGT TAATGGGCAG
    ATAATTCTTT TAAAGGGCTA TTCTGTGGAT GTCGACACAG AAGCCAGGGT AATTCAGAGA
    GCAGCTGCCT ATCACACAGC TTGGAGAGAA GCTAAAGGAG AATCTGGGTT GCTACCACTG
    ATATTCTTAT TTTTGTGTAA GTCGGATCCT ATGGCTTATC TGATGGTCCC ATATTATCCT
    AGGGCAAATC TGAATGCTGT TCAGTCCACC ATGCCTTTAA CTTCAGAAGA AGTTTTAAAG
    GTCATGAAAG GTGTAGCCCA GGGCCTTCAT ACATTGCACA AGGCTGACAT AGTTCATGGA
    TCACTTCATC AGAACAATGT TTTTGCTTTA AACCGTCAAC ATGGAATTGT TGGAGATTTT
    GACTTCACCA AATCTGTGAG TCAGCGAACC TCAGTGAACA TGATGGTTGG TGACTTAAGT
    TTGCTATCAC CTGAACTGAA AATGGGAAAA CCTGCTTCTC CGAGTTCTGA CTTATATGCT
    TATGGCTGCC TTTTATTGTG GCTTTCAGTT CAGAATCAGG AATTTGAAAC TAATGAGGAT
    GGAATCCCAA AGATGGATCA GTTTCATTTG GATGATAATG TCAAATCCCT CCTCTGTAGC
    TTGATCTGTT GTAGAAGTTC AATGACTGCC GAGCAGGTTT TGAATGCTGA CTGTTTCTTG
    CTACCAAAGG GAAAATCAAT GCCAAACTCA GAGAAAGATA CTGAATACAT CCCCAAAAAA
    GAGGAAGAAG AATTAAAGGT GAAGAACTTG GATAAAGATA AGGAAAAGAC AAGAAATGGT
    GAAGCCAGCT TTGATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015325201.1
    CDS80..3697
    Translation

    Target ORF information:

    RefSeq Version XM_015469715.1
    Organism Bos taurus(cattle)
    Definition Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015469715.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGAGCGGC TGACGTTACC TCCTGGCGGC GCGGCGGCAG TGGACGAGTA CTTGGAGTAC 
    CGGAGAATTG TTGGTGAGGA TGACGGAGGG AAACTTTTTA CTCCTGAGGA ATATGAAGAA
    TACAAAAGAA AAGTCTTACC TATGCGTTTA CAGAACAGAT TGTATGTGAG TTGGCAGTCA
    CCCACGGGAA TGGATTGTAA ACTTGTGGGT CCAGAGACAC TGTGTTTTTG TACACATAGG
    TATAAGCAAC ATAAAACTGA CTTTGAAACG ATTCCCCCGC AGCGCCCCAT CAGTCTGCCT
    TGCCAAGTGA GGGGCTGCCC TTGCCGGGCT TACGTCTACG TGCCTCTGAA CGGCACACAG
    CCCATTCGCT GCAGATGTAA GCACTTTGCC GATCAACACA GTGCAGCGCC GGGCTTTGTA
    TGCAATGCCT GTTCTAAGTG TTCGGGATTT CATAGTTGCT TTACTTGTGC TTGTGGTCAG
    CCTGCATATG CCCATGACAC AGTAGTGGAA ACTAAGCAAG AAAGACTGGC TCAGGGAAAA
    CCAGTGGGGC AGGACGTTCC TTATGCAGCA ATGGGAGGAT TAACTGGCTT CAGCTCGCTG
    GCAGAAGGCT ACATACGATT AGATGACAGT GGAATTGGTG CACCATCAGC TGAATTTTTA
    GACTCTCCAG TTATGGCCAT GGACCACCCA TTTCTAAAGG CATTTCAAGC ATCATCTAGC
    TCTTCTCCAG AAACGCTAAC AGGAATTGTC CAAATGGAGG AAGATAAACA TTGCAATAAA
    GTTGAAGATG TGGTTGGAAG TCATGTGGAA GATGCAGTAA CATTTTGGGC CCAGAGTATC
    AATAGAAATA AGGATATTAT GAAGATTGGT TGTTCACTGT CTGAAGTCTG TCCCCTTGCC
    AATTCAGTGT TTGGGAATCT TGATCCAAAG AAGATTTATG GTGGATTATT TTCTGAAGAT
    AAATGTTGGT ACAGATGCCA AGTACTGAAA GTCATCAACG ATGAAAAGTG TCTGGTGAGA
    TATATTGACT ATGGAAATAC AGAAATTCTG AATCGATCGG ATATAGTAGA GATTCCTTTG
    GATCTGCAAT TTTCTAGTGT TGCCAAAAAG TATAGACTTT GGGGACTACA GATTCCTTCT
    AGCCAAGAAG TTACCCAGTT TGATCAGGGC AGAACCTTTT TGGGGAGCTT GATTTTTGAA
    AAGGAAATTA AAATGAGAAT TAAAGCTACC TATCAAGATG GAACAATAAT TGCTCAGGCT
    GAGTATGGCA ATGTAGATAT TGGGGAAGAG GTGGCTAAGA AAGGATTTGC AGAAAAATGC
    AGACTCACTT CCAGCACTAA TATCTGTGAG GAAAAAAAAT CAGATCCTGG TCAGCTTGTC
    CTCAGGAACC TCAAAAGCCC CATTCCTTTG TGGGGGCATA GATCAAGCCA ATCAAACTTC
    ACCCGGCCAA AGGGGCGCTT AAGTGGGAGA ATGTGTCTTG ACTTGAAGAA TGAAAATGAT
    GCAGGAAATA ATGTGACATT TCCAAAGGAA AATTTGGCTG TTGGTGACTT TAATTTAGGG
    TCTAATGTCA GCCTGGCAAA AATTAAGCAG GACCAGAAAC TGATTGAAGA AAATGAAAAA
    CTTAAAACAG AGAAGGAGGT TCTTCTTGAA AGGTGCAAGG CATTAGAATT GAAAGTCGAA
    CAGACTGCCC AGGAGCTGCA GCAAGAGAAA GCAACTACCA TGGATTTGAC TAAACGTTTG
    GAAAGCACTG TGAAGACTTG TATAGGTACC AGAATGAAAA ATCTGGCAGC TAAGGTTGAA
    ATATTGAAAG AAATTAGACG TGTCAGTATC AGTGTTCGTT TTGGAAATGA CCTTTCAGAT
    GCTATACAAG TGTTGGATGA AGACTGCTTT ACTATCCCAG CTTCTTTGAA TGAATTAGAG
    CTAATTTGGG CAGAATATAA TCTGGCTCAG GAGAAGATTC AAACTTGTGA GAATGTGGAT
    TTGTCAGCCT CTTTAGAAGC AGTATATGGA CAGGCCAAAG AAGGAATTAA CTCTGAAGAA
    ATACTGGAGA AATTTAATGA CTGGAGGTGT AATAAAAGAG AGGAGTTCAC CAGTGTTAGA
    AGTGAAACAG AGACTTGTAT ACAACGTCTT ATAGCATGGT TCCAGAATAC TTTAAAGGCC
    TTTGATCTGT CTGTGGACAA ATCACTGATT TCTGATGACA CAATTGATAA TATTGATGAA
    ATCCTGGAGA ACACTGAGTC AGGTGTCTGC AAGGAGCTGG AGATATCTCT GATTGAACAA
    GGTGATGCAG ACAAGGAGAT CATTTTAAAA ACATGTAGTC AAGTACTACA AAAGATCCGT
    TCAGAGGAAA GCCTTATTGC CACAGTACAA TCCAAGTACA AGGACAGTGT TGAGTTTAAG
    AAACAGATTA TTGAATGTTT AAATAAGAGC CCCAGCGTGG ATCACTTGCT TTCCATTAAA
    AAGACACTGA AAAGCTTAAA GGCTCGACTG AGATGGAAAT TGGTTGAAAA GAATAATTTG
    GAAGAATCTG ATGACCATGA TGGATCTGAG ATTGAAAAAA TAAAACAAGA AATAACTCAG
    TTACGGAATA GTGTCTTTGA GGGAATTTAT CATGAGCAGG AGGAATATGC GAAGCTGAGT
    AGCTTAGTAC AAAAATGGTT CCCTGAGCTT CCTCTACTTC ATCCTGAAAT AGGATTACTT
    AAGTACATGA ACTCTGGTGG TCTTCTTACA ATGAGCTTGG AAAGAGATCT TCTTGATGCT
    GAGCCTATGA AGGAGCTTAG CAGCAAGCGT CCTTTGATAT GTTCTGAGGT TAATGGGCAG
    ATAATTCTTT TAAAGGGCTA TTCTGTGGAT GTCGACACAG AAGCCAGGGT AATTCAGAGA
    GCAGCTGCCT ATCACACAGC TTGGAGAGAA GCTAAAGGAG AATCTGGGTT GCTACCACTG
    ATATTCTTAT TTTTGTGTAA GTCGGATCCT ATGGCTTATC TGATGGTCCC ATATTATCCT
    AGGGCAAATC TGAATGCTGT TCAGTCCACC ATGCCTTTAA CTTCAGAAGA AGTTTTAAAG
    GTCATGAAAG GTGTAGCCCA GGGCCTTCAT ACATTGCACA AGGCTGACAT AGTTCATGGA
    TCACTTCATC AGAACAATGT TTTTGCTTTA AACCGTCAAC ATGGAATTGT TGGAGATTTT
    GACTTCACCA AATCTGTGAG TCAGCGAACC TCAGTGAACA TGATGGTTGG TGACTTAAGT
    TTGCTATCAC CTGAACTGAA AATGGGAAAA CCTGCTTCTC CGAGTTCTGA CTTATATGCT
    TATGGCTGCC TTTTATTGTG GCTTTCAGTT CAGAATCAGG AATTTGAAAC TAATGAGGAT
    GGAATCCCAA AGATGGATCA GTTTCATTTG GATGATAATG TCAAATCCCT CCTCTGTAGC
    TTGATCTGTT GTAGAAGTTC AATGACTGCC GAGCAGGTTT TGAATGCTGA CTGTTTCTTG
    CTACCAAAGG GAAAATCAAT GCCAAACTCA GAGAAAGATA CTGAATACAT CCCCAAAAAA
    GAGGAAGAAG AATTAAAGGT GAAGAACTTG GATAAAGATA AGGAAAAGAC AAGAAATGGT
    GAAGCCAGCT TTGATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90624
    Clone ID Related Accession (Same CDS sequence) XM_024990535.1 , XM_015469716.1 , XM_015469716.2
    Accession Version XM_024990535.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3600bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein FAM221A isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCGTTTAC AGAACAGATT GTATGTGAGT TGGCAGTCAC CCACGGGAAT GGATTGTAAA 
    CTTGTGGGTC CAGAGACACT GTGTTTTTGT ACACATAGGT ATAAGCAACA TAAAACTGAC
    TTTGAAACGA TTCCCCCGCA GCGCCCCATC AGTCTGCCTT GCCAAGTGAG GGGCTGCCCT
    TGCCGGGCTT ACGTCTACGT GCCTCTGAAC GGCACACAGC CCATTCGCTG CAGATGTAAG
    CACTTTGCCG ATCAACACAG TGCAGCGCCG GGCTTTGTAT GCAATGCCTG TTCTAAGTGT
    TCGGGATTTC ATAGTTGCTT TACTTGTGCT TGTGGTCAGC CTGCATATGC CCATGACACA
    GTAGTGGAAA CTAAGCAAGA AAGACTGGCT CAGGGAAAAC CAGTGGGGCA GGACGTTCCT
    TATGCAGCAA TGGGAGGATT AACTGGCTTC AGCTCGCTGG CAGAAGGCTA CATACGATTA
    GATGACAGTG GAATTGGTGC ACCATCAGCT GAATTTTTAG ACTCTCCAGT TATGGCCATG
    GACCACCCAT TTCTAAAGGC ATTTCAAGCA TCATCTAGCT CTTCTCCAGA AACGCTAACA
    GGAATTGTCC AAATGGAGGA AGATAAACAT TGCAATAAAG TTGAAGATGT GGTTGGAAGT
    CATGTGGAAG ATGCAGTAAC ATTTTGGGCC CAGAGTATCA ATAGAAATAA GGATATTATG
    AAGATTGGTT GTTCACTGTC TGAAGTCTGT CCCCTTGCCA ATTCAGTGTT TGGGAATCTT
    GATCCAAAGA AGATTTATGG TGGATTATTT TCTGAAGATA AATGTTGGTA CAGATGCCAA
    GTACTGAAAG TCATCAACGA TGAAAAGTGT CTGGTGAGAT ATATTGACTA TGGAAATACA
    GAAATTCTGA ATCGATCGGA TATAGTAGAG ATTCCTTTGG ATCTGCAATT TTCTAGTGTT
    GCCAAAAAGT ATAGACTTTG GGGACTACAG ATTCCTTCTA GCCAAGAAGT TACCCAGTTT
    GATCAGGGCA GAACCTTTTT GGGGAGCTTG ATTTTTGAAA AGGAAATTAA AATGAGAATT
    AAAGCTACCT ATCAAGATGG AACAATAATT GCTCAGGCTG AGTATGGCAA TGTAGATATT
    GGGGAAGAGG TGGCTAAGAA AGGATTTGCA GAAAAATGCA GACTCACTTC CAGCACTAAT
    ATCTGTGAGG AAAAAAAATC AGATCCTGGT CAGCTTGTCC TCAGGAACCT CAAAAGCCCC
    ATTCCTTTGT GGGGGCATAG ATCAAGCCAA TCAAACTTCA CCCGGCCAAA GGGGCGCTTA
    AGTGGGAGAA TGTGTCTTGA CTTGAAGAAT GAAAATGATG CAGGAAATAA TGTGACATTT
    CCAAAGGAAA ATTTGGCTGT TGGTGACTTT AATTTAGGGT CTAATGTCAG CCTGGCAAAA
    ATTAAGCAGG ACCAGAAACT GATTGAAGAA AATGAAAAAC TTAAAACAGA GAAGGAGGTT
    CTTCTTGAAA GGTGCAAGGC ATTAGAATTG AAAGTCGAAC AGACTGCCCA GGAGCTGCAG
    CAAGAGAAAG CAACTACCAT GGATTTGACT AAACGTTTGG AAAGCACTGT GAAGACTTGT
    ATAGGTACCA GAATGAAAAA TCTGGCAGCT AAGGTTGAAA TATTGAAAGA AATTAGACGT
    GTCAGTATCA GTGTTCGTTT TGGAAATGAC CTTTCAGATG CTATACAAGT GTTGGATGAA
    GACTGCTTTA CTATCCCAGC TTCTTTGAAT GAATTAGAGC TAATTTGGGC AGAATATAAT
    CTGGCTCAGG AGAAGATTCA AACTTGTGAG AATGTGAATG AAGGGAATAT TTTGATTGCT
    GAAAGAAATG AAGTACAACA GAAGCTGTAC ATGGCAGTAG AAGTTTTTAT TCTGGAAGTT
    GATGAGTTAT CTCTTAATAA ACGCTTAAAA ACGTTGCAGG ATTTGTCAGC CTCTTTAGAA
    GCAGTATATG GACAGGCCAA AGAAGGAATT AACTCTGAAG AAATACTGGA GAAATTTAAT
    GACTGGAGGT GTAATAAAAG AGAGGAGTTC ACCAGTGTTA GAAGTGAAAC AGAGACTTGT
    ATACAACGTC TTATAGCATG GTTCCAGAAT ACTTTAAAGG CCTTTGATCT GTCTGTGGAC
    AAATCACTGA TTTCTGATGA CACAATTGAT AATATTGATG AAATCCTGGA GAACACTGAG
    TCAGGTGTCT GCAAGGAGCT GGAGATATCT CTGATTGAAC AAGGTGATGC AGACAAGGAG
    ATCATTTTAA AAACATGTAG TCAAGTACTA CAAAAGATCC GTTCAGAGGA AAGCCTTATT
    GCCACAGTAC AATCCAAGTA CAAGGACAGT GTTGAGTTTA AGAAACAGAT TATTGAATGT
    TTAAATAAGA GCCCCAGCGT GGATCACTTG CTTTCCATTA AAAAGACACT GAAAAGCTTA
    AAGGCTCGAC TGAGATGGAA ATTGGTTGAA AAGAATAATT TGGAAGAATC TGATGACCAT
    GATGGATCTG AGATTGAAAA AATAAAACAA GAAATAACTC AGTTACGGAA TAGTGTCTTT
    GAGGGAATTT ATCATGAGCA GGAGGAATAT GCGAAGCTGA GTAGCTTAGT ACAAAAATGG
    TTCCCTGAGC TTCCTCTACT TCATCCTGAA ATAGGATTAC TTAAGTACAT GAACTCTGGT
    GGTCTTCTTA CAATGAGCTT GGAAAGAGAT CTTCTTGATG CTGAGCCTAT GAAGGAGCTT
    AGCAGCAAGC GTCCTTTGAT ATGTTCTGAG GTTAATGGGC AGATAATTCT TTTAAAGGGC
    TATTCTGTGG ATGTCGACAC AGAAGCCAGG GTAATTCAGA GAGCAGCTGC CTATCACACA
    GCTTGGAGAG AAGCTAAAGG AGAATCTGGG TTGCTACCAC TGATATTCTT ATTTTTGTGT
    AAGTCGGATC CTATGGCTTA TCTGATGGTC CCATATTATC CTAGGGCAAA TCTGAATGCT
    GTTCAGTCCA CCATGCCTTT AACTTCAGAA GAAGTTTTAA AGGTCATGAA AGGTGTAGCC
    CAGGGCCTTC ATACATTGCA CAAGGCTGAC ATAGTTCATG GATCACTTCA TCAGAACAAT
    GTTTTTGCTT TAAACCGTCA ACATGGAATT GTTGGAGATT TTGACTTCAC CAAATCTGTG
    AGTCAGCGAA CCTCAGTGAA CATGATGGTT GGTGACTTAA GTTTGCTATC ACCTGAACTG
    AAAATGGGAA AACCTGCTTC TCCGAGTTCT GACTTATATG CTTATGGCTG CCTTTTATTG
    TGGCTTTCAG TTCAGAATCA GGAATTTGAA ACTAATGAGG ATGGAATCCC AAAGATGGAT
    CAGTTTCATT TGGATGATAA TGTCAAATCC CTCCTCTGTA GCTTGATCTG TTGTAGAAGT
    TCAATGACTG CCGAGCAGGT TTTGAATGCT GACTGTTTCT TGCTACCAAA GGGAAAATCA
    ATGCCAAACT CAGAGAAAGA TACTGAATAC ATCCCCAAAA AAGAGGAAGA AGAATTAAAG
    GTGAAGAACT TGGATAAAGA TAAGGAAAAG ACAAGAAATG GTGAAGCCAG CTTTGATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024846303.1
    CDS406..4005
    Translation

    Target ORF information:

    RefSeq Version XM_024990535.1
    Organism Bos taurus(cattle)
    Definition Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024990535.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCGTTTAC AGAACAGATT GTATGTGAGT TGGCAGTCAC CCACGGGAAT GGATTGTAAA 
    CTTGTGGGTC CAGAGACACT GTGTTTTTGT ACACATAGGT ATAAGCAACA TAAAACTGAC
    TTTGAAACGA TTCCCCCGCA GCGCCCCATC AGTCTGCCTT GCCAAGTGAG GGGCTGCCCT
    TGCCGGGCTT ACGTCTACGT GCCTCTGAAC GGCACACAGC CCATTCGCTG CAGATGTAAG
    CACTTTGCCG ATCAACACAG TGCAGCGCCG GGCTTTGTAT GCAATGCCTG TTCTAAGTGT
    TCGGGATTTC ATAGTTGCTT TACTTGTGCT TGTGGTCAGC CTGCATATGC CCATGACACA
    GTAGTGGAAA CTAAGCAAGA AAGACTGGCT CAGGGAAAAC CAGTGGGGCA GGACGTTCCT
    TATGCAGCAA TGGGAGGATT AACTGGCTTC AGCTCGCTGG CAGAAGGCTA CATACGATTA
    GATGACAGTG GAATTGGTGC ACCATCAGCT GAATTTTTAG ACTCTCCAGT TATGGCCATG
    GACCACCCAT TTCTAAAGGC ATTTCAAGCA TCATCTAGCT CTTCTCCAGA AACGCTAACA
    GGAATTGTCC AAATGGAGGA AGATAAACAT TGCAATAAAG TTGAAGATGT GGTTGGAAGT
    CATGTGGAAG ATGCAGTAAC ATTTTGGGCC CAGAGTATCA ATAGAAATAA GGATATTATG
    AAGATTGGTT GTTCACTGTC TGAAGTCTGT CCCCTTGCCA ATTCAGTGTT TGGGAATCTT
    GATCCAAAGA AGATTTATGG TGGATTATTT TCTGAAGATA AATGTTGGTA CAGATGCCAA
    GTACTGAAAG TCATCAACGA TGAAAAGTGT CTGGTGAGAT ATATTGACTA TGGAAATACA
    GAAATTCTGA ATCGATCGGA TATAGTAGAG ATTCCTTTGG ATCTGCAATT TTCTAGTGTT
    GCCAAAAAGT ATAGACTTTG GGGACTACAG ATTCCTTCTA GCCAAGAAGT TACCCAGTTT
    GATCAGGGCA GAACCTTTTT GGGGAGCTTG ATTTTTGAAA AGGAAATTAA AATGAGAATT
    AAAGCTACCT ATCAAGATGG AACAATAATT GCTCAGGCTG AGTATGGCAA TGTAGATATT
    GGGGAAGAGG TGGCTAAGAA AGGATTTGCA GAAAAATGCA GACTCACTTC CAGCACTAAT
    ATCTGTGAGG AAAAAAAATC AGATCCTGGT CAGCTTGTCC TCAGGAACCT CAAAAGCCCC
    ATTCCTTTGT GGGGGCATAG ATCAAGCCAA TCAAACTTCA CCCGGCCAAA GGGGCGCTTA
    AGTGGGAGAA TGTGTCTTGA CTTGAAGAAT GAAAATGATG CAGGAAATAA TGTGACATTT
    CCAAAGGAAA ATTTGGCTGT TGGTGACTTT AATTTAGGGT CTAATGTCAG CCTGGCAAAA
    ATTAAGCAGG ACCAGAAACT GATTGAAGAA AATGAAAAAC TTAAAACAGA GAAGGAGGTT
    CTTCTTGAAA GGTGCAAGGC ATTAGAATTG AAAGTCGAAC AGACTGCCCA GGAGCTGCAG
    CAAGAGAAAG CAACTACCAT GGATTTGACT AAACGTTTGG AAAGCACTGT GAAGACTTGT
    ATAGGTACCA GAATGAAAAA TCTGGCAGCT AAGGTTGAAA TATTGAAAGA AATTAGACGT
    GTCAGTATCA GTGTTCGTTT TGGAAATGAC CTTTCAGATG CTATACAAGT GTTGGATGAA
    GACTGCTTTA CTATCCCAGC TTCTTTGAAT GAATTAGAGC TAATTTGGGC AGAATATAAT
    CTGGCTCAGG AGAAGATTCA AACTTGTGAG AATGTGAATG AAGGGAATAT TTTGATTGCT
    GAAAGAAATG AAGTACAACA GAAGCTGTAC ATGGCAGTAG AAGTTTTTAT TCTGGAAGTT
    GATGAGTTAT CTCTTAATAA ACGCTTAAAA ACGTTGCAGG ATTTGTCAGC CTCTTTAGAA
    GCAGTATATG GACAGGCCAA AGAAGGAATT AACTCTGAAG AAATACTGGA GAAATTTAAT
    GACTGGAGGT GTAATAAAAG AGAGGAGTTC ACCAGTGTTA GAAGTGAAAC AGAGACTTGT
    ATACAACGTC TTATAGCATG GTTCCAGAAT ACTTTAAAGG CCTTTGATCT GTCTGTGGAC
    AAATCACTGA TTTCTGATGA CACAATTGAT AATATTGATG AAATCCTGGA GAACACTGAG
    TCAGGTGTCT GCAAGGAGCT GGAGATATCT CTGATTGAAC AAGGTGATGC AGACAAGGAG
    ATCATTTTAA AAACATGTAG TCAAGTACTA CAAAAGATCC GTTCAGAGGA AAGCCTTATT
    GCCACAGTAC AATCCAAGTA CAAGGACAGT GTTGAGTTTA AGAAACAGAT TATTGAATGT
    TTAAATAAGA GCCCCAGCGT GGATCACTTG CTTTCCATTA AAAAGACACT GAAAAGCTTA
    AAGGCTCGAC TGAGATGGAA ATTGGTTGAA AAGAATAATT TGGAAGAATC TGATGACCAT
    GATGGATCTG AGATTGAAAA AATAAAACAA GAAATAACTC AGTTACGGAA TAGTGTCTTT
    GAGGGAATTT ATCATGAGCA GGAGGAATAT GCGAAGCTGA GTAGCTTAGT ACAAAAATGG
    TTCCCTGAGC TTCCTCTACT TCATCCTGAA ATAGGATTAC TTAAGTACAT GAACTCTGGT
    GGTCTTCTTA CAATGAGCTT GGAAAGAGAT CTTCTTGATG CTGAGCCTAT GAAGGAGCTT
    AGCAGCAAGC GTCCTTTGAT ATGTTCTGAG GTTAATGGGC AGATAATTCT TTTAAAGGGC
    TATTCTGTGG ATGTCGACAC AGAAGCCAGG GTAATTCAGA GAGCAGCTGC CTATCACACA
    GCTTGGAGAG AAGCTAAAGG AGAATCTGGG TTGCTACCAC TGATATTCTT ATTTTTGTGT
    AAGTCGGATC CTATGGCTTA TCTGATGGTC CCATATTATC CTAGGGCAAA TCTGAATGCT
    GTTCAGTCCA CCATGCCTTT AACTTCAGAA GAAGTTTTAA AGGTCATGAA AGGTGTAGCC
    CAGGGCCTTC ATACATTGCA CAAGGCTGAC ATAGTTCATG GATCACTTCA TCAGAACAAT
    GTTTTTGCTT TAAACCGTCA ACATGGAATT GTTGGAGATT TTGACTTCAC CAAATCTGTG
    AGTCAGCGAA CCTCAGTGAA CATGATGGTT GGTGACTTAA GTTTGCTATC ACCTGAACTG
    AAAATGGGAA AACCTGCTTC TCCGAGTTCT GACTTATATG CTTATGGCTG CCTTTTATTG
    TGGCTTTCAG TTCAGAATCA GGAATTTGAA ACTAATGAGG ATGGAATCCC AAAGATGGAT
    CAGTTTCATT TGGATGATAA TGTCAAATCC CTCCTCTGTA GCTTGATCTG TTGTAGAAGT
    TCAATGACTG CCGAGCAGGT TTTGAATGCT GACTGTTTCT TGCTACCAAA GGGAAAATCA
    ATGCCAAACT CAGAGAAAGA TACTGAATAC ATCCCCAAAA AAGAGGAAGA AGAATTAAAG
    GTGAAGAACT TGGATAAAGA TAAGGAAAAG ACAAGAAATG GTGAAGCCAG CTTTGATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90624
    Clone ID Related Accession (Same CDS sequence) XM_024990535.1 , XM_015469716.1 , XM_015469716.2
    Accession Version XM_015469716.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3600bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein FAM221A isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103900.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCGTTTAC AGAACAGATT GTATGTGAGT TGGCAGTCAC CCACGGGAAT GGATTGTAAA 
    CTTGTGGGTC CAGAGACACT GTGTTTTTGT ACACATAGGT ATAAGCAACA TAAAACTGAC
    TTTGAAACGA TTCCCCCGCA GCGCCCCATC AGTCTGCCTT GCCAAGTGAG GGGCTGCCCT
    TGCCGGGCTT ACGTCTACGT GCCTCTGAAC GGCACACAGC CCATTCGCTG CAGATGTAAG
    CACTTTGCCG ATCAACACAG TGCAGCGCCG GGCTTTGTAT GCAATGCCTG TTCTAAGTGT
    TCGGGATTTC ATAGTTGCTT TACTTGTGCT TGTGGTCAGC CTGCATATGC CCATGACACA
    GTAGTGGAAA CTAAGCAAGA AAGACTGGCT CAGGGAAAAC CAGTGGGGCA GGACGTTCCT
    TATGCAGCAA TGGGAGGATT AACTGGCTTC AGCTCGCTGG CAGAAGGCTA CATACGATTA
    GATGACAGTG GAATTGGTGC ACCATCAGCT GAATTTTTAG ACTCTCCAGT TATGGCCATG
    GACCACCCAT TTCTAAAGGC ATTTCAAGCA TCATCTAGCT CTTCTCCAGA AACGCTAACA
    GGAATTGTCC AAATGGAGGA AGATAAACAT TGCAATAAAG TTGAAGATGT GGTTGGAAGT
    CATGTGGAAG ATGCAGTAAC ATTTTGGGCC CAGAGTATCA ATAGAAATAA GGATATTATG
    AAGATTGGTT GTTCACTGTC TGAAGTCTGT CCCCTTGCCA ATTCAGTGTT TGGGAATCTT
    GATCCAAAGA AGATTTATGG TGGATTATTT TCTGAAGATA AATGTTGGTA CAGATGCCAA
    GTACTGAAAG TCATCAACGA TGAAAAGTGT CTGGTGAGAT ATATTGACTA TGGAAATACA
    GAAATTCTGA ATCGATCGGA TATAGTAGAG ATTCCTTTGG ATCTGCAATT TTCTAGTGTT
    GCCAAAAAGT ATAGACTTTG GGGACTACAG ATTCCTTCTA GCCAAGAAGT TACCCAGTTT
    GATCAGGGCA GAACCTTTTT GGGGAGCTTG ATTTTTGAAA AGGAAATTAA AATGAGAATT
    AAAGCTACCT ATCAAGATGG AACAATAATT GCTCAGGCTG AGTATGGCAA TGTAGATATT
    GGGGAAGAGG TGGCTAAGAA AGGATTTGCA GAAAAATGCA GACTCACTTC CAGCACTAAT
    ATCTGTGAGG AAAAAAAATC AGATCCTGGT CAGCTTGTCC TCAGGAACCT CAAAAGCCCC
    ATTCCTTTGT GGGGGCATAG ATCAAGCCAA TCAAACTTCA CCCGGCCAAA GGGGCGCTTA
    AGTGGGAGAA TGTGTCTTGA CTTGAAGAAT GAAAATGATG CAGGAAATAA TGTGACATTT
    CCAAAGGAAA ATTTGGCTGT TGGTGACTTT AATTTAGGGT CTAATGTCAG CCTGGCAAAA
    ATTAAGCAGG ACCAGAAACT GATTGAAGAA AATGAAAAAC TTAAAACAGA GAAGGAGGTT
    CTTCTTGAAA GGTGCAAGGC ATTAGAATTG AAAGTCGAAC AGACTGCCCA GGAGCTGCAG
    CAAGAGAAAG CAACTACCAT GGATTTGACT AAACGTTTGG AAAGCACTGT GAAGACTTGT
    ATAGGTACCA GAATGAAAAA TCTGGCAGCT AAGGTTGAAA TATTGAAAGA AATTAGACGT
    GTCAGTATCA GTGTTCGTTT TGGAAATGAC CTTTCAGATG CTATACAAGT GTTGGATGAA
    GACTGCTTTA CTATCCCAGC TTCTTTGAAT GAATTAGAGC TAATTTGGGC AGAATATAAT
    CTGGCTCAGG AGAAGATTCA AACTTGTGAG AATGTGAATG AAGGGAATAT TTTGATTGCT
    GAAAGAAATG AAGTACAACA GAAGCTGTAC ATGGCAGTAG AAGTTTTTAT TCTGGAAGTT
    GATGAGTTAT CTCTTAATAA ACGCTTAAAA ACGTTGCAGG ATTTGTCAGC CTCTTTAGAA
    GCAGTATATG GACAGGCCAA AGAAGGAATT AACTCTGAAG AAATACTGGA GAAATTTAAT
    GACTGGAGGT GTAATAAAAG AGAGGAGTTC ACCAGTGTTA GAAGTGAAAC AGAGACTTGT
    ATACAACGTC TTATAGCATG GTTCCAGAAT ACTTTAAAGG CCTTTGATCT GTCTGTGGAC
    AAATCACTGA TTTCTGATGA CACAATTGAT AATATTGATG AAATCCTGGA GAACACTGAG
    TCAGGTGTCT GCAAGGAGCT GGAGATATCT CTGATTGAAC AAGGTGATGC AGACAAGGAG
    ATCATTTTAA AAACATGTAG TCAAGTACTA CAAAAGATCC GTTCAGAGGA AAGCCTTATT
    GCCACAGTAC AATCCAAGTA CAAGGACAGT GTTGAGTTTA AGAAACAGAT TATTGAATGT
    TTAAATAAGA GCCCCAGCGT GGATCACTTG CTTTCCATTA AAAAGACACT GAAAAGCTTA
    AAGGCTCGAC TGAGATGGAA ATTGGTTGAA AAGAATAATT TGGAAGAATC TGATGACCAT
    GATGGATCTG AGATTGAAAA AATAAAACAA GAAATAACTC AGTTACGGAA TAGTGTCTTT
    GAGGGAATTT ATCATGAGCA GGAGGAATAT GCGAAGCTGA GTAGCTTAGT ACAAAAATGG
    TTCCCTGAGC TTCCTCTACT TCATCCTGAA ATAGGATTAC TTAAGTACAT GAACTCTGGT
    GGTCTTCTTA CAATGAGCTT GGAAAGAGAT CTTCTTGATG CTGAGCCTAT GAAGGAGCTT
    AGCAGCAAGC GTCCTTTGAT ATGTTCTGAG GTTAATGGGC AGATAATTCT TTTAAAGGGC
    TATTCTGTGG ATGTCGACAC AGAAGCCAGG GTAATTCAGA GAGCAGCTGC CTATCACACA
    GCTTGGAGAG AAGCTAAAGG AGAATCTGGG TTGCTACCAC TGATATTCTT ATTTTTGTGT
    AAGTCGGATC CTATGGCTTA TCTGATGGTC CCATATTATC CTAGGGCAAA TCTGAATGCT
    GTTCAGTCCA CCATGCCTTT AACTTCAGAA GAAGTTTTAA AGGTCATGAA AGGTGTAGCC
    CAGGGCCTTC ATACATTGCA CAAGGCTGAC ATAGTTCATG GATCACTTCA TCAGAACAAT
    GTTTTTGCTT TAAACCGTCA ACATGGAATT GTTGGAGATT TTGACTTCAC CAAATCTGTG
    AGTCAGCGAA CCTCAGTGAA CATGATGGTT GGTGACTTAA GTTTGCTATC ACCTGAACTG
    AAAATGGGAA AACCTGCTTC TCCGAGTTCT GACTTATATG CTTATGGCTG CCTTTTATTG
    TGGCTTTCAG TTCAGAATCA GGAATTTGAA ACTAATGAGG ATGGAATCCC AAAGATGGAT
    CAGTTTCATT TGGATGATAA TGTCAAATCC CTCCTCTGTA GCTTGATCTG TTGTAGAAGT
    TCAATGACTG CCGAGCAGGT TTTGAATGCT GACTGTTTCT TGCTACCAAA GGGAAAATCA
    ATGCCAAACT CAGAGAAAGA TACTGAATAC ATCCCCAAAA AAGAGGAAGA AGAATTAAAG
    GTGAAGAACT TGGATAAAGA TAAGGAAAAG ACAAGAAATG GTGAAGCCAG CTTTGATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015325202.1
    CDS255..3854
    Translation

    Target ORF information:

    RefSeq Version XM_015469716.1
    Organism Bos taurus(cattle)
    Definition Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015469716.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCGTTTAC AGAACAGATT GTATGTGAGT TGGCAGTCAC CCACGGGAAT GGATTGTAAA 
    CTTGTGGGTC CAGAGACACT GTGTTTTTGT ACACATAGGT ATAAGCAACA TAAAACTGAC
    TTTGAAACGA TTCCCCCGCA GCGCCCCATC AGTCTGCCTT GCCAAGTGAG GGGCTGCCCT
    TGCCGGGCTT ACGTCTACGT GCCTCTGAAC GGCACACAGC CCATTCGCTG CAGATGTAAG
    CACTTTGCCG ATCAACACAG TGCAGCGCCG GGCTTTGTAT GCAATGCCTG TTCTAAGTGT
    TCGGGATTTC ATAGTTGCTT TACTTGTGCT TGTGGTCAGC CTGCATATGC CCATGACACA
    GTAGTGGAAA CTAAGCAAGA AAGACTGGCT CAGGGAAAAC CAGTGGGGCA GGACGTTCCT
    TATGCAGCAA TGGGAGGATT AACTGGCTTC AGCTCGCTGG CAGAAGGCTA CATACGATTA
    GATGACAGTG GAATTGGTGC ACCATCAGCT GAATTTTTAG ACTCTCCAGT TATGGCCATG
    GACCACCCAT TTCTAAAGGC ATTTCAAGCA TCATCTAGCT CTTCTCCAGA AACGCTAACA
    GGAATTGTCC AAATGGAGGA AGATAAACAT TGCAATAAAG TTGAAGATGT GGTTGGAAGT
    CATGTGGAAG ATGCAGTAAC ATTTTGGGCC CAGAGTATCA ATAGAAATAA GGATATTATG
    AAGATTGGTT GTTCACTGTC TGAAGTCTGT CCCCTTGCCA ATTCAGTGTT TGGGAATCTT
    GATCCAAAGA AGATTTATGG TGGATTATTT TCTGAAGATA AATGTTGGTA CAGATGCCAA
    GTACTGAAAG TCATCAACGA TGAAAAGTGT CTGGTGAGAT ATATTGACTA TGGAAATACA
    GAAATTCTGA ATCGATCGGA TATAGTAGAG ATTCCTTTGG ATCTGCAATT TTCTAGTGTT
    GCCAAAAAGT ATAGACTTTG GGGACTACAG ATTCCTTCTA GCCAAGAAGT TACCCAGTTT
    GATCAGGGCA GAACCTTTTT GGGGAGCTTG ATTTTTGAAA AGGAAATTAA AATGAGAATT
    AAAGCTACCT ATCAAGATGG AACAATAATT GCTCAGGCTG AGTATGGCAA TGTAGATATT
    GGGGAAGAGG TGGCTAAGAA AGGATTTGCA GAAAAATGCA GACTCACTTC CAGCACTAAT
    ATCTGTGAGG AAAAAAAATC AGATCCTGGT CAGCTTGTCC TCAGGAACCT CAAAAGCCCC
    ATTCCTTTGT GGGGGCATAG ATCAAGCCAA TCAAACTTCA CCCGGCCAAA GGGGCGCTTA
    AGTGGGAGAA TGTGTCTTGA CTTGAAGAAT GAAAATGATG CAGGAAATAA TGTGACATTT
    CCAAAGGAAA ATTTGGCTGT TGGTGACTTT AATTTAGGGT CTAATGTCAG CCTGGCAAAA
    ATTAAGCAGG ACCAGAAACT GATTGAAGAA AATGAAAAAC TTAAAACAGA GAAGGAGGTT
    CTTCTTGAAA GGTGCAAGGC ATTAGAATTG AAAGTCGAAC AGACTGCCCA GGAGCTGCAG
    CAAGAGAAAG CAACTACCAT GGATTTGACT AAACGTTTGG AAAGCACTGT GAAGACTTGT
    ATAGGTACCA GAATGAAAAA TCTGGCAGCT AAGGTTGAAA TATTGAAAGA AATTAGACGT
    GTCAGTATCA GTGTTCGTTT TGGAAATGAC CTTTCAGATG CTATACAAGT GTTGGATGAA
    GACTGCTTTA CTATCCCAGC TTCTTTGAAT GAATTAGAGC TAATTTGGGC AGAATATAAT
    CTGGCTCAGG AGAAGATTCA AACTTGTGAG AATGTGAATG AAGGGAATAT TTTGATTGCT
    GAAAGAAATG AAGTACAACA GAAGCTGTAC ATGGCAGTAG AAGTTTTTAT TCTGGAAGTT
    GATGAGTTAT CTCTTAATAA ACGCTTAAAA ACGTTGCAGG ATTTGTCAGC CTCTTTAGAA
    GCAGTATATG GACAGGCCAA AGAAGGAATT AACTCTGAAG AAATACTGGA GAAATTTAAT
    GACTGGAGGT GTAATAAAAG AGAGGAGTTC ACCAGTGTTA GAAGTGAAAC AGAGACTTGT
    ATACAACGTC TTATAGCATG GTTCCAGAAT ACTTTAAAGG CCTTTGATCT GTCTGTGGAC
    AAATCACTGA TTTCTGATGA CACAATTGAT AATATTGATG AAATCCTGGA GAACACTGAG
    TCAGGTGTCT GCAAGGAGCT GGAGATATCT CTGATTGAAC AAGGTGATGC AGACAAGGAG
    ATCATTTTAA AAACATGTAG TCAAGTACTA CAAAAGATCC GTTCAGAGGA AAGCCTTATT
    GCCACAGTAC AATCCAAGTA CAAGGACAGT GTTGAGTTTA AGAAACAGAT TATTGAATGT
    TTAAATAAGA GCCCCAGCGT GGATCACTTG CTTTCCATTA AAAAGACACT GAAAAGCTTA
    AAGGCTCGAC TGAGATGGAA ATTGGTTGAA AAGAATAATT TGGAAGAATC TGATGACCAT
    GATGGATCTG AGATTGAAAA AATAAAACAA GAAATAACTC AGTTACGGAA TAGTGTCTTT
    GAGGGAATTT ATCATGAGCA GGAGGAATAT GCGAAGCTGA GTAGCTTAGT ACAAAAATGG
    TTCCCTGAGC TTCCTCTACT TCATCCTGAA ATAGGATTAC TTAAGTACAT GAACTCTGGT
    GGTCTTCTTA CAATGAGCTT GGAAAGAGAT CTTCTTGATG CTGAGCCTAT GAAGGAGCTT
    AGCAGCAAGC GTCCTTTGAT ATGTTCTGAG GTTAATGGGC AGATAATTCT TTTAAAGGGC
    TATTCTGTGG ATGTCGACAC AGAAGCCAGG GTAATTCAGA GAGCAGCTGC CTATCACACA
    GCTTGGAGAG AAGCTAAAGG AGAATCTGGG TTGCTACCAC TGATATTCTT ATTTTTGTGT
    AAGTCGGATC CTATGGCTTA TCTGATGGTC CCATATTATC CTAGGGCAAA TCTGAATGCT
    GTTCAGTCCA CCATGCCTTT AACTTCAGAA GAAGTTTTAA AGGTCATGAA AGGTGTAGCC
    CAGGGCCTTC ATACATTGCA CAAGGCTGAC ATAGTTCATG GATCACTTCA TCAGAACAAT
    GTTTTTGCTT TAAACCGTCA ACATGGAATT GTTGGAGATT TTGACTTCAC CAAATCTGTG
    AGTCAGCGAA CCTCAGTGAA CATGATGGTT GGTGACTTAA GTTTGCTATC ACCTGAACTG
    AAAATGGGAA AACCTGCTTC TCCGAGTTCT GACTTATATG CTTATGGCTG CCTTTTATTG
    TGGCTTTCAG TTCAGAATCA GGAATTTGAA ACTAATGAGG ATGGAATCCC AAAGATGGAT
    CAGTTTCATT TGGATGATAA TGTCAAATCC CTCCTCTGTA GCTTGATCTG TTGTAGAAGT
    TCAATGACTG CCGAGCAGGT TTTGAATGCT GACTGTTTCT TGCTACCAAA GGGAAAATCA
    ATGCCAAACT CAGAGAAAGA TACTGAATAC ATCCCCAAAA AAGAGGAAGA AGAATTAAAG
    GTGAAGAACT TGGATAAAGA TAAGGAAAAG ACAAGAAATG GTGAAGCCAG CTTTGATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90624
    Clone ID Related Accession (Same CDS sequence) XM_024990535.1 , XM_015469716.1 , XM_015469716.2
    Accession Version XM_015469716.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3600bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein FAM221A isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015469716.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCGTTTAC AGAACAGATT GTATGTGAGT TGGCAGTCAC CCACGGGAAT GGATTGTAAA 
    CTTGTGGGTC CAGAGACACT GTGTTTTTGT ACACATAGGT ATAAGCAACA TAAAACTGAC
    TTTGAAACGA TTCCCCCGCA GCGCCCCATC AGTCTGCCTT GCCAAGTGAG GGGCTGCCCT
    TGCCGGGCTT ACGTCTACGT GCCTCTGAAC GGCACACAGC CCATTCGCTG CAGATGTAAG
    CACTTTGCCG ATCAACACAG TGCAGCGCCG GGCTTTGTAT GCAATGCCTG TTCTAAGTGT
    TCGGGATTTC ATAGTTGCTT TACTTGTGCT TGTGGTCAGC CTGCATATGC CCATGACACA
    GTAGTGGAAA CTAAGCAAGA AAGACTGGCT CAGGGAAAAC CAGTGGGGCA GGACGTTCCT
    TATGCAGCAA TGGGAGGATT AACTGGCTTC AGCTCGCTGG CAGAAGGCTA CATACGATTA
    GATGACAGTG GAATTGGTGC ACCATCAGCT GAATTTTTAG ACTCTCCAGT TATGGCCATG
    GACCACCCAT TTCTAAAGGC ATTTCAAGCA TCATCTAGCT CTTCTCCAGA AACGCTAACA
    GGAATTGTCC AAATGGAGGA AGATAAACAT TGCAATAAAG TTGAAGATGT GGTTGGAAGT
    CATGTGGAAG ATGCAGTAAC ATTTTGGGCC CAGAGTATCA ATAGAAATAA GGATATTATG
    AAGATTGGTT GTTCACTGTC TGAAGTCTGT CCCCTTGCCA ATTCAGTGTT TGGGAATCTT
    GATCCAAAGA AGATTTATGG TGGATTATTT TCTGAAGATA AATGTTGGTA CAGATGCCAA
    GTACTGAAAG TCATCAACGA TGAAAAGTGT CTGGTGAGAT ATATTGACTA TGGAAATACA
    GAAATTCTGA ATCGATCGGA TATAGTAGAG ATTCCTTTGG ATCTGCAATT TTCTAGTGTT
    GCCAAAAAGT ATAGACTTTG GGGACTACAG ATTCCTTCTA GCCAAGAAGT TACCCAGTTT
    GATCAGGGCA GAACCTTTTT GGGGAGCTTG ATTTTTGAAA AGGAAATTAA AATGAGAATT
    AAAGCTACCT ATCAAGATGG AACAATAATT GCTCAGGCTG AGTATGGCAA TGTAGATATT
    GGGGAAGAGG TGGCTAAGAA AGGATTTGCA GAAAAATGCA GACTCACTTC CAGCACTAAT
    ATCTGTGAGG AAAAAAAATC AGATCCTGGT CAGCTTGTCC TCAGGAACCT CAAAAGCCCC
    ATTCCTTTGT GGGGGCATAG ATCAAGCCAA TCAAACTTCA CCCGGCCAAA GGGGCGCTTA
    AGTGGGAGAA TGTGTCTTGA CTTGAAGAAT GAAAATGATG CAGGAAATAA TGTGACATTT
    CCAAAGGAAA ATTTGGCTGT TGGTGACTTT AATTTAGGGT CTAATGTCAG CCTGGCAAAA
    ATTAAGCAGG ACCAGAAACT GATTGAAGAA AATGAAAAAC TTAAAACAGA GAAGGAGGTT
    CTTCTTGAAA GGTGCAAGGC ATTAGAATTG AAAGTCGAAC AGACTGCCCA GGAGCTGCAG
    CAAGAGAAAG CAACTACCAT GGATTTGACT AAACGTTTGG AAAGCACTGT GAAGACTTGT
    ATAGGTACCA GAATGAAAAA TCTGGCAGCT AAGGTTGAAA TATTGAAAGA AATTAGACGT
    GTCAGTATCA GTGTTCGTTT TGGAAATGAC CTTTCAGATG CTATACAAGT GTTGGATGAA
    GACTGCTTTA CTATCCCAGC TTCTTTGAAT GAATTAGAGC TAATTTGGGC AGAATATAAT
    CTGGCTCAGG AGAAGATTCA AACTTGTGAG AATGTGAATG AAGGGAATAT TTTGATTGCT
    GAAAGAAATG AAGTACAACA GAAGCTGTAC ATGGCAGTAG AAGTTTTTAT TCTGGAAGTT
    GATGAGTTAT CTCTTAATAA ACGCTTAAAA ACGTTGCAGG ATTTGTCAGC CTCTTTAGAA
    GCAGTATATG GACAGGCCAA AGAAGGAATT AACTCTGAAG AAATACTGGA GAAATTTAAT
    GACTGGAGGT GTAATAAAAG AGAGGAGTTC ACCAGTGTTA GAAGTGAAAC AGAGACTTGT
    ATACAACGTC TTATAGCATG GTTCCAGAAT ACTTTAAAGG CCTTTGATCT GTCTGTGGAC
    AAATCACTGA TTTCTGATGA CACAATTGAT AATATTGATG AAATCCTGGA GAACACTGAG
    TCAGGTGTCT GCAAGGAGCT GGAGATATCT CTGATTGAAC AAGGTGATGC AGACAAGGAG
    ATCATTTTAA AAACATGTAG TCAAGTACTA CAAAAGATCC GTTCAGAGGA AAGCCTTATT
    GCCACAGTAC AATCCAAGTA CAAGGACAGT GTTGAGTTTA AGAAACAGAT TATTGAATGT
    TTAAATAAGA GCCCCAGCGT GGATCACTTG CTTTCCATTA AAAAGACACT GAAAAGCTTA
    AAGGCTCGAC TGAGATGGAA ATTGGTTGAA AAGAATAATT TGGAAGAATC TGATGACCAT
    GATGGATCTG AGATTGAAAA AATAAAACAA GAAATAACTC AGTTACGGAA TAGTGTCTTT
    GAGGGAATTT ATCATGAGCA GGAGGAATAT GCGAAGCTGA GTAGCTTAGT ACAAAAATGG
    TTCCCTGAGC TTCCTCTACT TCATCCTGAA ATAGGATTAC TTAAGTACAT GAACTCTGGT
    GGTCTTCTTA CAATGAGCTT GGAAAGAGAT CTTCTTGATG CTGAGCCTAT GAAGGAGCTT
    AGCAGCAAGC GTCCTTTGAT ATGTTCTGAG GTTAATGGGC AGATAATTCT TTTAAAGGGC
    TATTCTGTGG ATGTCGACAC AGAAGCCAGG GTAATTCAGA GAGCAGCTGC CTATCACACA
    GCTTGGAGAG AAGCTAAAGG AGAATCTGGG TTGCTACCAC TGATATTCTT ATTTTTGTGT
    AAGTCGGATC CTATGGCTTA TCTGATGGTC CCATATTATC CTAGGGCAAA TCTGAATGCT
    GTTCAGTCCA CCATGCCTTT AACTTCAGAA GAAGTTTTAA AGGTCATGAA AGGTGTAGCC
    CAGGGCCTTC ATACATTGCA CAAGGCTGAC ATAGTTCATG GATCACTTCA TCAGAACAAT
    GTTTTTGCTT TAAACCGTCA ACATGGAATT GTTGGAGATT TTGACTTCAC CAAATCTGTG
    AGTCAGCGAA CCTCAGTGAA CATGATGGTT GGTGACTTAA GTTTGCTATC ACCTGAACTG
    AAAATGGGAA AACCTGCTTC TCCGAGTTCT GACTTATATG CTTATGGCTG CCTTTTATTG
    TGGCTTTCAG TTCAGAATCA GGAATTTGAA ACTAATGAGG ATGGAATCCC AAAGATGGAT
    CAGTTTCATT TGGATGATAA TGTCAAATCC CTCCTCTGTA GCTTGATCTG TTGTAGAAGT
    TCAATGACTG CCGAGCAGGT TTTGAATGCT GACTGTTTCT TGCTACCAAA GGGAAAATCA
    ATGCCAAACT CAGAGAAAGA TACTGAATAC ATCCCCAAAA AAGAGGAAGA AGAATTAAAG
    GTGAAGAACT TGGATAAAGA TAAGGAAAAG ACAAGAAATG GTGAAGCCAG CTTTGATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015325202.1
    CDS391..3990
    Translation

    Target ORF information:

    RefSeq Version XM_015469716.2
    Organism Bos taurus(cattle)
    Definition Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015469716.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCGTTTAC AGAACAGATT GTATGTGAGT TGGCAGTCAC CCACGGGAAT GGATTGTAAA 
    CTTGTGGGTC CAGAGACACT GTGTTTTTGT ACACATAGGT ATAAGCAACA TAAAACTGAC
    TTTGAAACGA TTCCCCCGCA GCGCCCCATC AGTCTGCCTT GCCAAGTGAG GGGCTGCCCT
    TGCCGGGCTT ACGTCTACGT GCCTCTGAAC GGCACACAGC CCATTCGCTG CAGATGTAAG
    CACTTTGCCG ATCAACACAG TGCAGCGCCG GGCTTTGTAT GCAATGCCTG TTCTAAGTGT
    TCGGGATTTC ATAGTTGCTT TACTTGTGCT TGTGGTCAGC CTGCATATGC CCATGACACA
    GTAGTGGAAA CTAAGCAAGA AAGACTGGCT CAGGGAAAAC CAGTGGGGCA GGACGTTCCT
    TATGCAGCAA TGGGAGGATT AACTGGCTTC AGCTCGCTGG CAGAAGGCTA CATACGATTA
    GATGACAGTG GAATTGGTGC ACCATCAGCT GAATTTTTAG ACTCTCCAGT TATGGCCATG
    GACCACCCAT TTCTAAAGGC ATTTCAAGCA TCATCTAGCT CTTCTCCAGA AACGCTAACA
    GGAATTGTCC AAATGGAGGA AGATAAACAT TGCAATAAAG TTGAAGATGT GGTTGGAAGT
    CATGTGGAAG ATGCAGTAAC ATTTTGGGCC CAGAGTATCA ATAGAAATAA GGATATTATG
    AAGATTGGTT GTTCACTGTC TGAAGTCTGT CCCCTTGCCA ATTCAGTGTT TGGGAATCTT
    GATCCAAAGA AGATTTATGG TGGATTATTT TCTGAAGATA AATGTTGGTA CAGATGCCAA
    GTACTGAAAG TCATCAACGA TGAAAAGTGT CTGGTGAGAT ATATTGACTA TGGAAATACA
    GAAATTCTGA ATCGATCGGA TATAGTAGAG ATTCCTTTGG ATCTGCAATT TTCTAGTGTT
    GCCAAAAAGT ATAGACTTTG GGGACTACAG ATTCCTTCTA GCCAAGAAGT TACCCAGTTT
    GATCAGGGCA GAACCTTTTT GGGGAGCTTG ATTTTTGAAA AGGAAATTAA AATGAGAATT
    AAAGCTACCT ATCAAGATGG AACAATAATT GCTCAGGCTG AGTATGGCAA TGTAGATATT
    GGGGAAGAGG TGGCTAAGAA AGGATTTGCA GAAAAATGCA GACTCACTTC CAGCACTAAT
    ATCTGTGAGG AAAAAAAATC AGATCCTGGT CAGCTTGTCC TCAGGAACCT CAAAAGCCCC
    ATTCCTTTGT GGGGGCATAG ATCAAGCCAA TCAAACTTCA CCCGGCCAAA GGGGCGCTTA
    AGTGGGAGAA TGTGTCTTGA CTTGAAGAAT GAAAATGATG CAGGAAATAA TGTGACATTT
    CCAAAGGAAA ATTTGGCTGT TGGTGACTTT AATTTAGGGT CTAATGTCAG CCTGGCAAAA
    ATTAAGCAGG ACCAGAAACT GATTGAAGAA AATGAAAAAC TTAAAACAGA GAAGGAGGTT
    CTTCTTGAAA GGTGCAAGGC ATTAGAATTG AAAGTCGAAC AGACTGCCCA GGAGCTGCAG
    CAAGAGAAAG CAACTACCAT GGATTTGACT AAACGTTTGG AAAGCACTGT GAAGACTTGT
    ATAGGTACCA GAATGAAAAA TCTGGCAGCT AAGGTTGAAA TATTGAAAGA AATTAGACGT
    GTCAGTATCA GTGTTCGTTT TGGAAATGAC CTTTCAGATG CTATACAAGT GTTGGATGAA
    GACTGCTTTA CTATCCCAGC TTCTTTGAAT GAATTAGAGC TAATTTGGGC AGAATATAAT
    CTGGCTCAGG AGAAGATTCA AACTTGTGAG AATGTGAATG AAGGGAATAT TTTGATTGCT
    GAAAGAAATG AAGTACAACA GAAGCTGTAC ATGGCAGTAG AAGTTTTTAT TCTGGAAGTT
    GATGAGTTAT CTCTTAATAA ACGCTTAAAA ACGTTGCAGG ATTTGTCAGC CTCTTTAGAA
    GCAGTATATG GACAGGCCAA AGAAGGAATT AACTCTGAAG AAATACTGGA GAAATTTAAT
    GACTGGAGGT GTAATAAAAG AGAGGAGTTC ACCAGTGTTA GAAGTGAAAC AGAGACTTGT
    ATACAACGTC TTATAGCATG GTTCCAGAAT ACTTTAAAGG CCTTTGATCT GTCTGTGGAC
    AAATCACTGA TTTCTGATGA CACAATTGAT AATATTGATG AAATCCTGGA GAACACTGAG
    TCAGGTGTCT GCAAGGAGCT GGAGATATCT CTGATTGAAC AAGGTGATGC AGACAAGGAG
    ATCATTTTAA AAACATGTAG TCAAGTACTA CAAAAGATCC GTTCAGAGGA AAGCCTTATT
    GCCACAGTAC AATCCAAGTA CAAGGACAGT GTTGAGTTTA AGAAACAGAT TATTGAATGT
    TTAAATAAGA GCCCCAGCGT GGATCACTTG CTTTCCATTA AAAAGACACT GAAAAGCTTA
    AAGGCTCGAC TGAGATGGAA ATTGGTTGAA AAGAATAATT TGGAAGAATC TGATGACCAT
    GATGGATCTG AGATTGAAAA AATAAAACAA GAAATAACTC AGTTACGGAA TAGTGTCTTT
    GAGGGAATTT ATCATGAGCA GGAGGAATAT GCGAAGCTGA GTAGCTTAGT ACAAAAATGG
    TTCCCTGAGC TTCCTCTACT TCATCCTGAA ATAGGATTAC TTAAGTACAT GAACTCTGGT
    GGTCTTCTTA CAATGAGCTT GGAAAGAGAT CTTCTTGATG CTGAGCCTAT GAAGGAGCTT
    AGCAGCAAGC GTCCTTTGAT ATGTTCTGAG GTTAATGGGC AGATAATTCT TTTAAAGGGC
    TATTCTGTGG ATGTCGACAC AGAAGCCAGG GTAATTCAGA GAGCAGCTGC CTATCACACA
    GCTTGGAGAG AAGCTAAAGG AGAATCTGGG TTGCTACCAC TGATATTCTT ATTTTTGTGT
    AAGTCGGATC CTATGGCTTA TCTGATGGTC CCATATTATC CTAGGGCAAA TCTGAATGCT
    GTTCAGTCCA CCATGCCTTT AACTTCAGAA GAAGTTTTAA AGGTCATGAA AGGTGTAGCC
    CAGGGCCTTC ATACATTGCA CAAGGCTGAC ATAGTTCATG GATCACTTCA TCAGAACAAT
    GTTTTTGCTT TAAACCGTCA ACATGGAATT GTTGGAGATT TTGACTTCAC CAAATCTGTG
    AGTCAGCGAA CCTCAGTGAA CATGATGGTT GGTGACTTAA GTTTGCTATC ACCTGAACTG
    AAAATGGGAA AACCTGCTTC TCCGAGTTCT GACTTATATG CTTATGGCTG CCTTTTATTG
    TGGCTTTCAG TTCAGAATCA GGAATTTGAA ACTAATGAGG ATGGAATCCC AAAGATGGAT
    CAGTTTCATT TGGATGATAA TGTCAAATCC CTCCTCTGTA GCTTGATCTG TTGTAGAAGT
    TCAATGACTG CCGAGCAGGT TTTGAATGCT GACTGTTTCT TGCTACCAAA GGGAAAATCA
    ATGCCAAACT CAGAGAAAGA TACTGAATAC ATCCCCAAAA AAGAGGAAGA AGAATTAAAG
    GTGAAGAACT TGGATAAAGA TAAGGAAAAG ACAAGAAATG GTGAAGCCAG CTTTGATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90625
    Clone ID Related Accession (Same CDS sequence) XM_015469718.1
    Accession Version XM_015469718.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3591bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein FAM221A isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103900.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGAGCGGC TGACGTTACC TCCTGGCGGC GCGGCGGCAG TGGACGAGTA CTTGGAGTAC 
    CGGAGAATTG TTGGTGAGGA TGACGGAGGG AAACTTTTTA CTCCTGAGGA ATATGAAGAA
    TACAAAAGAA AAGTCTTACC TATGCGTTTA CAGAACAGAT TGTATGTGAG TTGGCAGTCA
    CCCACGGGAA TGGATTGTAA ACTTGTGGGT CCAGAGACAC TGTGTTTTTG TACACATAGG
    TATAAGCAAC ATAAAACTGA CTTTGAAACG ATTCCCCCGC AGCGCCCCAT CAGTCTGCCT
    TGCCAAGTGA GGGGCTGCCC TTGCCGGGCT TACGTCTACG TGCCTCTGAA CGGCACACAG
    CCCATTCGCT GCAGATGTAA GCACTTTGCC GATCAACACA GTGCAGCGCC GGGCTTTGTA
    TGCAATGCCT GTTCTAAGTG TTCGGGATTT CATAGTTGCT TTACTTGTGC TTGTGGTCAG
    CCTGCATATG CCCATGACAC AGTAGTGGAA ACTAAGCAAG AAAGACTGGC TCAGGGAAAA
    CCAGTGGGGC AGGACGTTCC TTATGCAGCA ATGGGAGGAT TAACTGGCTT CAGCTCGCTG
    GCAGAAGGCT ACATACGATT AGATGACAGT GGAATTGGTG CACCATCAGC TGAATTTTTA
    GACTCTCCAG TTATGGCCAT GGACCACCCA TTTCTAAAGG CATTTCAAGC ATCATCTAGC
    TCTTCTCCAG AAACGCTAAC AGGAATTGTC CAAATGGAGG AAGATAAACA TTGCAATAAA
    GTTGAAGATG TGGTTGGAAG TCATGTGGAA GATGCAGTAA CATTTTGGGC CCAGAGTATC
    AATAGAAATA AGGATATTAT GAAGATTGGT TGTTCACTGT CTGAAGTCTG TCCCCTTGCC
    AATTCAGTGT TTGGGAATCT TGATCCAAAG AAGATTTATG GTGGATTATT TTCTGAAGAT
    AAATGTTGGT ACAGATGCCA AGTACTGAAA GTCATCAACG ATGAAAAGTG TCTGGTGAGA
    TATATTGACT ATGGAAATAC AGAAATTCTG AATCGATCGG ATATAGTAGA GATTCCTTTG
    GATCTGCAAT TTTCTAGTGT TGCCAAAAAG TATAGACTTT GGGGACTACA GATTCCTTCT
    AGCCAAGAAG TTACCCAGTT TGATCAGGGC AGAACCTTTT TGGGGAGCTT GATTTTTGAA
    AAGGAAATTA AAATGAGAAT TAAAGCTACC TATCAAGATG GAACAATAAT TGCTCAGGCT
    GAGTATGGCA ATGTAGATAT TGGGGAAGAG GTGGCTAAGA AAGGATTTGC AGAAAAATGC
    AGACTCACTT CCAGCACTAA TATCTGTGAG GAAAAAAAAT CAGATCCTGG TCAGCTTGTC
    CTCAGGAACC TCAAAAGCCC CATTCCTTTG TGGGGGCATA GATCAAGCCA ATCAAACTTC
    ACCCGGCCAA AGGGGCGCTT AAGTGGGAGA ATGTGTCTTG ACTTGAAGAA TGAAAATGAT
    GCAGGAAATA ATGTGACATT TCCAAAGGAA AATTTGGCTG TTGGTGACTT TAATTTAGGG
    TCTAATGTCA GCCTGGCAAA AATTAAGCAG GACCAGAAAC TGATTGAAGA AAATGAAAAA
    CTTAAAACAG AGAAGGAGGT TCTTCTTGAA AGGTGCAAGG CATTAGAATT GAAAGTCGAA
    CAGACTGCCC AGGAGCTGCA GCAAGAGAAA GCAACTACCA TGGATTTGAC TAAACGTTTG
    GAAAGCACTG TGAAGACTTG TATAGGTACC AGAATGAAAA ATCTGGCAGC TAAGGTTGAA
    ATATTGAAAG AAATTAGACG TGTCAGTATC AGTGTTCGTT TTGGAAATGA CCTTTCAGAT
    GCTATACAAG TGTTGGATGA AGACTGCTTT ACTATCCCAG CTTCTTTGAA TGAATTAGAG
    CTAATTTGGG CAGAATATAA TCTGGCTCAG GAGAAGATTC AAACTTGTGA GAATGTGAAT
    GAAGGGAATA TTTTGATTGC TGAAAGAAAT GAAGTACAAC AGAAGCTGTA CATGGCAGTA
    GAAGTTTTTA TTCTGGAAGT TGATGAGTTA TCTCTTAATA AACGCTTAAA AACGTTGCAG
    GATTTGTCAG CCTCTTTAGA AGCAGTATAT GGACAGGCCA AAGAAGGAAT TAACTCTGAA
    GAAATACTGG AGAAATTTAA TGACTGGAGG TGTAATAAAA GAGAGGAGTT CACCAGTGTT
    AGAAGTGAAA CAGAGACTTG TATACAACGT CTTATAGCAT GGTTCCAGAA TACTTTAAAG
    GCCTTTGATC TGTCTGTGGA CAAATCACTG ATTTCTGATG ACACAATTGA TAATATTGAT
    GAAATCCTGG AGAACACTGA GTCAGGTGTC TGCAAGGAGC TGGAGATATC TCTGATTGAA
    CAAGGTGATG CAGACAAGGA GATCATTTTA AAAACATGTA GTCAAGTACT ACAAAAGATC
    CGTTCAGAGG AAAGCCTTAT TGCCACAGTA CAATCCAAGT ACAAGGACAG TGTTGAGTTT
    AAGAAACAGA TTATTGAATG TTTAAATAAG AGCCCCAGCG TGGATCACTT GCTTTCCATT
    AAAAAGACAC TGAAAAGCTT AAAGGCTCGA CTGAGATGGA AATTGGTTGA AAAGAATAAT
    TTGGAAGAAT CTGATGACCA TGATGGATCT GAGATTGAAA AAATAAAACA AGAAATAACT
    CAGTTACGGA ATAGTGTCTT TGAGGGAATT TATCATGAGC AGGAGGAATA TGCGAAGCTG
    AGTAGCTTAG TACAAAAATG GTTCCCTGAG CTTCCTCTAC TTCATCCTGA AATAGGATTA
    CTTAAGTACA TGAACTCTGG TGGTCTTCTT ACAATGAGCT TGGAAAGAGA TCTTCTTGAT
    GCTGAGCCTA TGAAGGAGCT TAGCAGCAAG CGTCCTTTGA TATGTTCTGA GGTTAATGGG
    CAGATAATTC TTTTAAAGGG CTATTCTGTG GATGTCGACA CAGAAGCCAG GGTAATTCAG
    AGAGCAGCTG CCTATCACAC AGCTTGGAGA GAAGCTAAAG GAGAATCTGG GTTGCTACCA
    CTGATATTCT TATTTTTGTG TAAGTCGGAT CCTATGGCTT ATCTGATGGT CCCATATTAT
    CCTAGGGCAA ATCTGAATGC TGTTCAGTCC ACCATGCCTT TAACTTCAGA AAGTCAGCGA
    ACCTCAGTGA ACATGATGGT TGGTGACTTA AGTTTGCTAT CACCTGAACT GAAAATGGGA
    AAACCTGCTT CTCCGAGTTC TGACTTATAT GCTTATGGCT GCCTTTTATT GTGGCTTTCA
    GTTCAGAATC AGGAATTTGA AACTAATGAG GATGGAATCC CAAAGATGGA TCAGTTTCAT
    TTGGATGATA ATGTCAAATC CCTCCTCTGT AGCTTGATCT GTTGTAGAAG TTCAATGACT
    GCCGAGCAGG TTTTGAATGC TGACTGTTTC TTGCTACCAA AGGGAAAATC AATGCCAAAC
    TCAGAGAAAG ATACTGAATA CATCCCCAAA AAAGAGGAAG AAGAATTAAA GGTGAAGAAC
    TTGGATAAAG ATAAGGAAAA GACAAGAAAT GGTGAAGCCA GCTTTGATTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015325204.1
    CDS80..3670
    Translation

    Target ORF information:

    RefSeq Version XM_015469718.1
    Organism Bos taurus(cattle)
    Definition Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015469718.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGAGCGGC TGACGTTACC TCCTGGCGGC GCGGCGGCAG TGGACGAGTA CTTGGAGTAC 
    CGGAGAATTG TTGGTGAGGA TGACGGAGGG AAACTTTTTA CTCCTGAGGA ATATGAAGAA
    TACAAAAGAA AAGTCTTACC TATGCGTTTA CAGAACAGAT TGTATGTGAG TTGGCAGTCA
    CCCACGGGAA TGGATTGTAA ACTTGTGGGT CCAGAGACAC TGTGTTTTTG TACACATAGG
    TATAAGCAAC ATAAAACTGA CTTTGAAACG ATTCCCCCGC AGCGCCCCAT CAGTCTGCCT
    TGCCAAGTGA GGGGCTGCCC TTGCCGGGCT TACGTCTACG TGCCTCTGAA CGGCACACAG
    CCCATTCGCT GCAGATGTAA GCACTTTGCC GATCAACACA GTGCAGCGCC GGGCTTTGTA
    TGCAATGCCT GTTCTAAGTG TTCGGGATTT CATAGTTGCT TTACTTGTGC TTGTGGTCAG
    CCTGCATATG CCCATGACAC AGTAGTGGAA ACTAAGCAAG AAAGACTGGC TCAGGGAAAA
    CCAGTGGGGC AGGACGTTCC TTATGCAGCA ATGGGAGGAT TAACTGGCTT CAGCTCGCTG
    GCAGAAGGCT ACATACGATT AGATGACAGT GGAATTGGTG CACCATCAGC TGAATTTTTA
    GACTCTCCAG TTATGGCCAT GGACCACCCA TTTCTAAAGG CATTTCAAGC ATCATCTAGC
    TCTTCTCCAG AAACGCTAAC AGGAATTGTC CAAATGGAGG AAGATAAACA TTGCAATAAA
    GTTGAAGATG TGGTTGGAAG TCATGTGGAA GATGCAGTAA CATTTTGGGC CCAGAGTATC
    AATAGAAATA AGGATATTAT GAAGATTGGT TGTTCACTGT CTGAAGTCTG TCCCCTTGCC
    AATTCAGTGT TTGGGAATCT TGATCCAAAG AAGATTTATG GTGGATTATT TTCTGAAGAT
    AAATGTTGGT ACAGATGCCA AGTACTGAAA GTCATCAACG ATGAAAAGTG TCTGGTGAGA
    TATATTGACT ATGGAAATAC AGAAATTCTG AATCGATCGG ATATAGTAGA GATTCCTTTG
    GATCTGCAAT TTTCTAGTGT TGCCAAAAAG TATAGACTTT GGGGACTACA GATTCCTTCT
    AGCCAAGAAG TTACCCAGTT TGATCAGGGC AGAACCTTTT TGGGGAGCTT GATTTTTGAA
    AAGGAAATTA AAATGAGAAT TAAAGCTACC TATCAAGATG GAACAATAAT TGCTCAGGCT
    GAGTATGGCA ATGTAGATAT TGGGGAAGAG GTGGCTAAGA AAGGATTTGC AGAAAAATGC
    AGACTCACTT CCAGCACTAA TATCTGTGAG GAAAAAAAAT CAGATCCTGG TCAGCTTGTC
    CTCAGGAACC TCAAAAGCCC CATTCCTTTG TGGGGGCATA GATCAAGCCA ATCAAACTTC
    ACCCGGCCAA AGGGGCGCTT AAGTGGGAGA ATGTGTCTTG ACTTGAAGAA TGAAAATGAT
    GCAGGAAATA ATGTGACATT TCCAAAGGAA AATTTGGCTG TTGGTGACTT TAATTTAGGG
    TCTAATGTCA GCCTGGCAAA AATTAAGCAG GACCAGAAAC TGATTGAAGA AAATGAAAAA
    CTTAAAACAG AGAAGGAGGT TCTTCTTGAA AGGTGCAAGG CATTAGAATT GAAAGTCGAA
    CAGACTGCCC AGGAGCTGCA GCAAGAGAAA GCAACTACCA TGGATTTGAC TAAACGTTTG
    GAAAGCACTG TGAAGACTTG TATAGGTACC AGAATGAAAA ATCTGGCAGC TAAGGTTGAA
    ATATTGAAAG AAATTAGACG TGTCAGTATC AGTGTTCGTT TTGGAAATGA CCTTTCAGAT
    GCTATACAAG TGTTGGATGA AGACTGCTTT ACTATCCCAG CTTCTTTGAA TGAATTAGAG
    CTAATTTGGG CAGAATATAA TCTGGCTCAG GAGAAGATTC AAACTTGTGA GAATGTGAAT
    GAAGGGAATA TTTTGATTGC TGAAAGAAAT GAAGTACAAC AGAAGCTGTA CATGGCAGTA
    GAAGTTTTTA TTCTGGAAGT TGATGAGTTA TCTCTTAATA AACGCTTAAA AACGTTGCAG
    GATTTGTCAG CCTCTTTAGA AGCAGTATAT GGACAGGCCA AAGAAGGAAT TAACTCTGAA
    GAAATACTGG AGAAATTTAA TGACTGGAGG TGTAATAAAA GAGAGGAGTT CACCAGTGTT
    AGAAGTGAAA CAGAGACTTG TATACAACGT CTTATAGCAT GGTTCCAGAA TACTTTAAAG
    GCCTTTGATC TGTCTGTGGA CAAATCACTG ATTTCTGATG ACACAATTGA TAATATTGAT
    GAAATCCTGG AGAACACTGA GTCAGGTGTC TGCAAGGAGC TGGAGATATC TCTGATTGAA
    CAAGGTGATG CAGACAAGGA GATCATTTTA AAAACATGTA GTCAAGTACT ACAAAAGATC
    CGTTCAGAGG AAAGCCTTAT TGCCACAGTA CAATCCAAGT ACAAGGACAG TGTTGAGTTT
    AAGAAACAGA TTATTGAATG TTTAAATAAG AGCCCCAGCG TGGATCACTT GCTTTCCATT
    AAAAAGACAC TGAAAAGCTT AAAGGCTCGA CTGAGATGGA AATTGGTTGA AAAGAATAAT
    TTGGAAGAAT CTGATGACCA TGATGGATCT GAGATTGAAA AAATAAAACA AGAAATAACT
    CAGTTACGGA ATAGTGTCTT TGAGGGAATT TATCATGAGC AGGAGGAATA TGCGAAGCTG
    AGTAGCTTAG TACAAAAATG GTTCCCTGAG CTTCCTCTAC TTCATCCTGA AATAGGATTA
    CTTAAGTACA TGAACTCTGG TGGTCTTCTT ACAATGAGCT TGGAAAGAGA TCTTCTTGAT
    GCTGAGCCTA TGAAGGAGCT TAGCAGCAAG CGTCCTTTGA TATGTTCTGA GGTTAATGGG
    CAGATAATTC TTTTAAAGGG CTATTCTGTG GATGTCGACA CAGAAGCCAG GGTAATTCAG
    AGAGCAGCTG CCTATCACAC AGCTTGGAGA GAAGCTAAAG GAGAATCTGG GTTGCTACCA
    CTGATATTCT TATTTTTGTG TAAGTCGGAT CCTATGGCTT ATCTGATGGT CCCATATTAT
    CCTAGGGCAA ATCTGAATGC TGTTCAGTCC ACCATGCCTT TAACTTCAGA AAGTCAGCGA
    ACCTCAGTGA ACATGATGGT TGGTGACTTA AGTTTGCTAT CACCTGAACT GAAAATGGGA
    AAACCTGCTT CTCCGAGTTC TGACTTATAT GCTTATGGCT GCCTTTTATT GTGGCTTTCA
    GTTCAGAATC AGGAATTTGA AACTAATGAG GATGGAATCC CAAAGATGGA TCAGTTTCAT
    TTGGATGATA ATGTCAAATC CCTCCTCTGT AGCTTGATCT GTTGTAGAAG TTCAATGACT
    GCCGAGCAGG TTTTGAATGC TGACTGTTTC TTGCTACCAA AGGGAAAATC AATGCCAAAC
    TCAGAGAAAG ATACTGAATA CATCCCCAAA AAAGAGGAAG AAGAATTAAA GGTGAAGAAC
    TTGGATAAAG ATAAGGAAAA GACAAGAAAT GGTGAAGCCA GCTTTGATTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90626
    Clone ID Related Accession (Same CDS sequence) XM_015469721.1 , XM_015469721.2 , XM_015469719.1 , XM_015469719.2
    Accession Version XM_015469721.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3171bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein FAM221A isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103900.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGGAGGAT TAACTGGCTT CAGCTCGCTG GCAGAAGGCT ACATACGATT AGATGACAGT 
    GGAATTGGTG CACCATCAGC TGAATTTTTA GACTCTCCAG TTATGGCCAT GGACCACCCA
    TTTCTAAAGG CATTTCAAGC ATCATCTAGC TCTTCTCCAG AAACGCTAAC AGGAATTGTC
    CAAATGGAGG AAGATAAACA TTGCAATAAA GTTGAAGATG TGGTTGGAAG TCATGTGGAA
    GATGCAGTAA CATTTTGGGC CCAGAGTATC AATAGAAATA AGGATATTAT GAAGATTGGT
    TGTTCACTGT CTGAAGTCTG TCCCCTTGCC AATTCAGTGT TTGGGAATCT TGATCCAAAG
    AAGATTTATG GTGGATTATT TTCTGAAGAT AAATGTTGGT ACAGATGCCA AGTACTGAAA
    GTCATCAACG ATGAAAAGTG TCTGGTGAGA TATATTGACT ATGGAAATAC AGAAATTCTG
    AATCGATCGG ATATAGTAGA GATTCCTTTG GATCTGCAAT TTTCTAGTGT TGCCAAAAAG
    TATAGACTTT GGGGACTACA GATTCCTTCT AGCCAAGAAG TTACCCAGTT TGATCAGGGC
    AGAACCTTTT TGGGGAGCTT GATTTTTGAA AAGGAAATTA AAATGAGAAT TAAAGCTACC
    TATCAAGATG GAACAATAAT TGCTCAGGCT GAGTATGGCA ATGTAGATAT TGGGGAAGAG
    GTGGCTAAGA AAGGATTTGC AGAAAAATGC AGACTCACTT CCAGCACTAA TATCTGTGAG
    GAAAAAAAAT CAGATCCTGG TCAGCTTGTC CTCAGGAACC TCAAAAGCCC CATTCCTTTG
    TGGGGGCATA GATCAAGCCA ATCAAACTTC ACCCGGCCAA AGGGGCGCTT AAGTGGGAGA
    ATGTGTCTTG ACTTGAAGAA TGAAAATGAT GCAGGAAATA ATGTGACATT TCCAAAGGAA
    AATTTGGCTG TTGGTGACTT TAATTTAGGG TCTAATGTCA GCCTGGCAAA AATTAAGCAG
    GACCAGAAAC TGATTGAAGA AAATGAAAAA CTTAAAACAG AGAAGGAGGT TCTTCTTGAA
    AGGTGCAAGG CATTAGAATT GAAAGTCGAA CAGACTGCCC AGGAGCTGCA GCAAGAGAAA
    GCAACTACCA TGGATTTGAC TAAACGTTTG GAAAGCACTG TGAAGACTTG TATAGGTACC
    AGAATGAAAA ATCTGGCAGC TAAGGTTGAA ATATTGAAAG AAATTAGACG TGTCAGTATC
    AGTGTTCGTT TTGGAAATGA CCTTTCAGAT GCTATACAAG TGTTGGATGA AGACTGCTTT
    ACTATCCCAG CTTCTTTGAA TGAATTAGAG CTAATTTGGG CAGAATATAA TCTGGCTCAG
    GAGAAGATTC AAACTTGTGA GAATGTGAAT GAAGGGAATA TTTTGATTGC TGAAAGAAAT
    GAAGTACAAC AGAAGCTGTA CATGGCAGTA GAAGTTTTTA TTCTGGAAGT TGATGAGTTA
    TCTCTTAATA AACGCTTAAA AACGTTGCAG GATTTGTCAG CCTCTTTAGA AGCAGTATAT
    GGACAGGCCA AAGAAGGAAT TAACTCTGAA GAAATACTGG AGAAATTTAA TGACTGGAGG
    TGTAATAAAA GAGAGGAGTT CACCAGTGTT AGAAGTGAAA CAGAGACTTG TATACAACGT
    CTTATAGCAT GGTTCCAGAA TACTTTAAAG GCCTTTGATC TGTCTGTGGA CAAATCACTG
    ATTTCTGATG ACACAATTGA TAATATTGAT GAAATCCTGG AGAACACTGA GTCAGGTGTC
    TGCAAGGAGC TGGAGATATC TCTGATTGAA CAAGGTGATG CAGACAAGGA GATCATTTTA
    AAAACATGTA GTCAAGTACT ACAAAAGATC CGTTCAGAGG AAAGCCTTAT TGCCACAGTA
    CAATCCAAGT ACAAGGACAG TGTTGAGTTT AAGAAACAGA TTATTGAATG TTTAAATAAG
    AGCCCCAGCG TGGATCACTT GCTTTCCATT AAAAAGACAC TGAAAAGCTT AAAGGCTCGA
    CTGAGATGGA AATTGGTTGA AAAGAATAAT TTGGAAGAAT CTGATGACCA TGATGGATCT
    GAGATTGAAA AAATAAAACA AGAAATAACT CAGTTACGGA ATAGTGTCTT TGAGGGAATT
    TATCATGAGC AGGAGGAATA TGCGAAGCTG AGTAGCTTAG TACAAAAATG GTTCCCTGAG
    CTTCCTCTAC TTCATCCTGA AATAGGATTA CTTAAGTACA TGAACTCTGG TGGTCTTCTT
    ACAATGAGCT TGGAAAGAGA TCTTCTTGAT GCTGAGCCTA TGAAGGAGCT TAGCAGCAAG
    CGTCCTTTGA TATGTTCTGA GGTTAATGGG CAGATAATTC TTTTAAAGGG CTATTCTGTG
    GATGTCGACA CAGAAGCCAG GGTAATTCAG AGAGCAGCTG CCTATCACAC AGCTTGGAGA
    GAAGCTAAAG GAGAATCTGG GTTGCTACCA CTGATATTCT TATTTTTGTG TAAGTCGGAT
    CCTATGGCTT ATCTGATGGT CCCATATTAT CCTAGGGCAA ATCTGAATGC TGTTCAGTCC
    ACCATGCCTT TAACTTCAGA AGAAGTTTTA AAGGTCATGA AAGGTGTAGC CCAGGGCCTT
    CATACATTGC ACAAGGCTGA CATAGTTCAT GGATCACTTC ATCAGAACAA TGTTTTTGCT
    TTAAACCGTC AACATGGAAT TGTTGGAGAT TTTGACTTCA CCAAATCTGT GAGTCAGCGA
    ACCTCAGTGA ACATGATGGT TGGTGACTTA AGTTTGCTAT CACCTGAACT GAAAATGGGA
    AAACCTGCTT CTCCGAGTTC TGACTTATAT GCTTATGGCT GCCTTTTATT GTGGCTTTCA
    GTTCAGAATC AGGAATTTGA AACTAATGAG GATGGAATCC CAAAGATGGA TCAGTTTCAT
    TTGGATGATA ATGTCAAATC CCTCCTCTGT AGCTTGATCT GTTGTAGAAG TTCAATGACT
    GCCGAGCAGG TTTTGAATGC TGACTGTTTC TTGCTACCAA AGGGAAAATC AATGCCAAAC
    TCAGAGAAAG ATACTGAATA CATCCCCAAA AAAGAGGAAG AAGAATTAAA GGTGAAGAAC
    TTGGATAAAG ATAAGGAAAA GACAAGAAAT GGTGAAGCCA GCTTTGATTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015325207.1
    CDS251..3421
    Translation

    Target ORF information:

    RefSeq Version XM_015469721.1
    Organism Bos taurus(cattle)
    Definition Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015469721.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGGAGGAT TAACTGGCTT CAGCTCGCTG GCAGAAGGCT ACATACGATT AGATGACAGT 
    GGAATTGGTG CACCATCAGC TGAATTTTTA GACTCTCCAG TTATGGCCAT GGACCACCCA
    TTTCTAAAGG CATTTCAAGC ATCATCTAGC TCTTCTCCAG AAACGCTAAC AGGAATTGTC
    CAAATGGAGG AAGATAAACA TTGCAATAAA GTTGAAGATG TGGTTGGAAG TCATGTGGAA
    GATGCAGTAA CATTTTGGGC CCAGAGTATC AATAGAAATA AGGATATTAT GAAGATTGGT
    TGTTCACTGT CTGAAGTCTG TCCCCTTGCC AATTCAGTGT TTGGGAATCT TGATCCAAAG
    AAGATTTATG GTGGATTATT TTCTGAAGAT AAATGTTGGT ACAGATGCCA AGTACTGAAA
    GTCATCAACG ATGAAAAGTG TCTGGTGAGA TATATTGACT ATGGAAATAC AGAAATTCTG
    AATCGATCGG ATATAGTAGA GATTCCTTTG GATCTGCAAT TTTCTAGTGT TGCCAAAAAG
    TATAGACTTT GGGGACTACA GATTCCTTCT AGCCAAGAAG TTACCCAGTT TGATCAGGGC
    AGAACCTTTT TGGGGAGCTT GATTTTTGAA AAGGAAATTA AAATGAGAAT TAAAGCTACC
    TATCAAGATG GAACAATAAT TGCTCAGGCT GAGTATGGCA ATGTAGATAT TGGGGAAGAG
    GTGGCTAAGA AAGGATTTGC AGAAAAATGC AGACTCACTT CCAGCACTAA TATCTGTGAG
    GAAAAAAAAT CAGATCCTGG TCAGCTTGTC CTCAGGAACC TCAAAAGCCC CATTCCTTTG
    TGGGGGCATA GATCAAGCCA ATCAAACTTC ACCCGGCCAA AGGGGCGCTT AAGTGGGAGA
    ATGTGTCTTG ACTTGAAGAA TGAAAATGAT GCAGGAAATA ATGTGACATT TCCAAAGGAA
    AATTTGGCTG TTGGTGACTT TAATTTAGGG TCTAATGTCA GCCTGGCAAA AATTAAGCAG
    GACCAGAAAC TGATTGAAGA AAATGAAAAA CTTAAAACAG AGAAGGAGGT TCTTCTTGAA
    AGGTGCAAGG CATTAGAATT GAAAGTCGAA CAGACTGCCC AGGAGCTGCA GCAAGAGAAA
    GCAACTACCA TGGATTTGAC TAAACGTTTG GAAAGCACTG TGAAGACTTG TATAGGTACC
    AGAATGAAAA ATCTGGCAGC TAAGGTTGAA ATATTGAAAG AAATTAGACG TGTCAGTATC
    AGTGTTCGTT TTGGAAATGA CCTTTCAGAT GCTATACAAG TGTTGGATGA AGACTGCTTT
    ACTATCCCAG CTTCTTTGAA TGAATTAGAG CTAATTTGGG CAGAATATAA TCTGGCTCAG
    GAGAAGATTC AAACTTGTGA GAATGTGAAT GAAGGGAATA TTTTGATTGC TGAAAGAAAT
    GAAGTACAAC AGAAGCTGTA CATGGCAGTA GAAGTTTTTA TTCTGGAAGT TGATGAGTTA
    TCTCTTAATA AACGCTTAAA AACGTTGCAG GATTTGTCAG CCTCTTTAGA AGCAGTATAT
    GGACAGGCCA AAGAAGGAAT TAACTCTGAA GAAATACTGG AGAAATTTAA TGACTGGAGG
    TGTAATAAAA GAGAGGAGTT CACCAGTGTT AGAAGTGAAA CAGAGACTTG TATACAACGT
    CTTATAGCAT GGTTCCAGAA TACTTTAAAG GCCTTTGATC TGTCTGTGGA CAAATCACTG
    ATTTCTGATG ACACAATTGA TAATATTGAT GAAATCCTGG AGAACACTGA GTCAGGTGTC
    TGCAAGGAGC TGGAGATATC TCTGATTGAA CAAGGTGATG CAGACAAGGA GATCATTTTA
    AAAACATGTA GTCAAGTACT ACAAAAGATC CGTTCAGAGG AAAGCCTTAT TGCCACAGTA
    CAATCCAAGT ACAAGGACAG TGTTGAGTTT AAGAAACAGA TTATTGAATG TTTAAATAAG
    AGCCCCAGCG TGGATCACTT GCTTTCCATT AAAAAGACAC TGAAAAGCTT AAAGGCTCGA
    CTGAGATGGA AATTGGTTGA AAAGAATAAT TTGGAAGAAT CTGATGACCA TGATGGATCT
    GAGATTGAAA AAATAAAACA AGAAATAACT CAGTTACGGA ATAGTGTCTT TGAGGGAATT
    TATCATGAGC AGGAGGAATA TGCGAAGCTG AGTAGCTTAG TACAAAAATG GTTCCCTGAG
    CTTCCTCTAC TTCATCCTGA AATAGGATTA CTTAAGTACA TGAACTCTGG TGGTCTTCTT
    ACAATGAGCT TGGAAAGAGA TCTTCTTGAT GCTGAGCCTA TGAAGGAGCT TAGCAGCAAG
    CGTCCTTTGA TATGTTCTGA GGTTAATGGG CAGATAATTC TTTTAAAGGG CTATTCTGTG
    GATGTCGACA CAGAAGCCAG GGTAATTCAG AGAGCAGCTG CCTATCACAC AGCTTGGAGA
    GAAGCTAAAG GAGAATCTGG GTTGCTACCA CTGATATTCT TATTTTTGTG TAAGTCGGAT
    CCTATGGCTT ATCTGATGGT CCCATATTAT CCTAGGGCAA ATCTGAATGC TGTTCAGTCC
    ACCATGCCTT TAACTTCAGA AGAAGTTTTA AAGGTCATGA AAGGTGTAGC CCAGGGCCTT
    CATACATTGC ACAAGGCTGA CATAGTTCAT GGATCACTTC ATCAGAACAA TGTTTTTGCT
    TTAAACCGTC AACATGGAAT TGTTGGAGAT TTTGACTTCA CCAAATCTGT GAGTCAGCGA
    ACCTCAGTGA ACATGATGGT TGGTGACTTA AGTTTGCTAT CACCTGAACT GAAAATGGGA
    AAACCTGCTT CTCCGAGTTC TGACTTATAT GCTTATGGCT GCCTTTTATT GTGGCTTTCA
    GTTCAGAATC AGGAATTTGA AACTAATGAG GATGGAATCC CAAAGATGGA TCAGTTTCAT
    TTGGATGATA ATGTCAAATC CCTCCTCTGT AGCTTGATCT GTTGTAGAAG TTCAATGACT
    GCCGAGCAGG TTTTGAATGC TGACTGTTTC TTGCTACCAA AGGGAAAATC AATGCCAAAC
    TCAGAGAAAG ATACTGAATA CATCCCCAAA AAAGAGGAAG AAGAATTAAA GGTGAAGAAC
    TTGGATAAAG ATAAGGAAAA GACAAGAAAT GGTGAAGCCA GCTTTGATTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90626
    Clone ID Related Accession (Same CDS sequence) XM_015469721.1 , XM_015469721.2 , XM_015469719.1 , XM_015469719.2
    Accession Version XM_015469721.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3171bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein FAM221A isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015469721.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGGAGGAT TAACTGGCTT CAGCTCGCTG GCAGAAGGCT ACATACGATT AGATGACAGT 
    GGAATTGGTG CACCATCAGC TGAATTTTTA GACTCTCCAG TTATGGCCAT GGACCACCCA
    TTTCTAAAGG CATTTCAAGC ATCATCTAGC TCTTCTCCAG AAACGCTAAC AGGAATTGTC
    CAAATGGAGG AAGATAAACA TTGCAATAAA GTTGAAGATG TGGTTGGAAG TCATGTGGAA
    GATGCAGTAA CATTTTGGGC CCAGAGTATC AATAGAAATA AGGATATTAT GAAGATTGGT
    TGTTCACTGT CTGAAGTCTG TCCCCTTGCC AATTCAGTGT TTGGGAATCT TGATCCAAAG
    AAGATTTATG GTGGATTATT TTCTGAAGAT AAATGTTGGT ACAGATGCCA AGTACTGAAA
    GTCATCAACG ATGAAAAGTG TCTGGTGAGA TATATTGACT ATGGAAATAC AGAAATTCTG
    AATCGATCGG ATATAGTAGA GATTCCTTTG GATCTGCAAT TTTCTAGTGT TGCCAAAAAG
    TATAGACTTT GGGGACTACA GATTCCTTCT AGCCAAGAAG TTACCCAGTT TGATCAGGGC
    AGAACCTTTT TGGGGAGCTT GATTTTTGAA AAGGAAATTA AAATGAGAAT TAAAGCTACC
    TATCAAGATG GAACAATAAT TGCTCAGGCT GAGTATGGCA ATGTAGATAT TGGGGAAGAG
    GTGGCTAAGA AAGGATTTGC AGAAAAATGC AGACTCACTT CCAGCACTAA TATCTGTGAG
    GAAAAAAAAT CAGATCCTGG TCAGCTTGTC CTCAGGAACC TCAAAAGCCC CATTCCTTTG
    TGGGGGCATA GATCAAGCCA ATCAAACTTC ACCCGGCCAA AGGGGCGCTT AAGTGGGAGA
    ATGTGTCTTG ACTTGAAGAA TGAAAATGAT GCAGGAAATA ATGTGACATT TCCAAAGGAA
    AATTTGGCTG TTGGTGACTT TAATTTAGGG TCTAATGTCA GCCTGGCAAA AATTAAGCAG
    GACCAGAAAC TGATTGAAGA AAATGAAAAA CTTAAAACAG AGAAGGAGGT TCTTCTTGAA
    AGGTGCAAGG CATTAGAATT GAAAGTCGAA CAGACTGCCC AGGAGCTGCA GCAAGAGAAA
    GCAACTACCA TGGATTTGAC TAAACGTTTG GAAAGCACTG TGAAGACTTG TATAGGTACC
    AGAATGAAAA ATCTGGCAGC TAAGGTTGAA ATATTGAAAG AAATTAGACG TGTCAGTATC
    AGTGTTCGTT TTGGAAATGA CCTTTCAGAT GCTATACAAG TGTTGGATGA AGACTGCTTT
    ACTATCCCAG CTTCTTTGAA TGAATTAGAG CTAATTTGGG CAGAATATAA TCTGGCTCAG
    GAGAAGATTC AAACTTGTGA GAATGTGAAT GAAGGGAATA TTTTGATTGC TGAAAGAAAT
    GAAGTACAAC AGAAGCTGTA CATGGCAGTA GAAGTTTTTA TTCTGGAAGT TGATGAGTTA
    TCTCTTAATA AACGCTTAAA AACGTTGCAG GATTTGTCAG CCTCTTTAGA AGCAGTATAT
    GGACAGGCCA AAGAAGGAAT TAACTCTGAA GAAATACTGG AGAAATTTAA TGACTGGAGG
    TGTAATAAAA GAGAGGAGTT CACCAGTGTT AGAAGTGAAA CAGAGACTTG TATACAACGT
    CTTATAGCAT GGTTCCAGAA TACTTTAAAG GCCTTTGATC TGTCTGTGGA CAAATCACTG
    ATTTCTGATG ACACAATTGA TAATATTGAT GAAATCCTGG AGAACACTGA GTCAGGTGTC
    TGCAAGGAGC TGGAGATATC TCTGATTGAA CAAGGTGATG CAGACAAGGA GATCATTTTA
    AAAACATGTA GTCAAGTACT ACAAAAGATC CGTTCAGAGG AAAGCCTTAT TGCCACAGTA
    CAATCCAAGT ACAAGGACAG TGTTGAGTTT AAGAAACAGA TTATTGAATG TTTAAATAAG
    AGCCCCAGCG TGGATCACTT GCTTTCCATT AAAAAGACAC TGAAAAGCTT AAAGGCTCGA
    CTGAGATGGA AATTGGTTGA AAAGAATAAT TTGGAAGAAT CTGATGACCA TGATGGATCT
    GAGATTGAAA AAATAAAACA AGAAATAACT CAGTTACGGA ATAGTGTCTT TGAGGGAATT
    TATCATGAGC AGGAGGAATA TGCGAAGCTG AGTAGCTTAG TACAAAAATG GTTCCCTGAG
    CTTCCTCTAC TTCATCCTGA AATAGGATTA CTTAAGTACA TGAACTCTGG TGGTCTTCTT
    ACAATGAGCT TGGAAAGAGA TCTTCTTGAT GCTGAGCCTA TGAAGGAGCT TAGCAGCAAG
    CGTCCTTTGA TATGTTCTGA GGTTAATGGG CAGATAATTC TTTTAAAGGG CTATTCTGTG
    GATGTCGACA CAGAAGCCAG GGTAATTCAG AGAGCAGCTG CCTATCACAC AGCTTGGAGA
    GAAGCTAAAG GAGAATCTGG GTTGCTACCA CTGATATTCT TATTTTTGTG TAAGTCGGAT
    CCTATGGCTT ATCTGATGGT CCCATATTAT CCTAGGGCAA ATCTGAATGC TGTTCAGTCC
    ACCATGCCTT TAACTTCAGA AGAAGTTTTA AAGGTCATGA AAGGTGTAGC CCAGGGCCTT
    CATACATTGC ACAAGGCTGA CATAGTTCAT GGATCACTTC ATCAGAACAA TGTTTTTGCT
    TTAAACCGTC AACATGGAAT TGTTGGAGAT TTTGACTTCA CCAAATCTGT GAGTCAGCGA
    ACCTCAGTGA ACATGATGGT TGGTGACTTA AGTTTGCTAT CACCTGAACT GAAAATGGGA
    AAACCTGCTT CTCCGAGTTC TGACTTATAT GCTTATGGCT GCCTTTTATT GTGGCTTTCA
    GTTCAGAATC AGGAATTTGA AACTAATGAG GATGGAATCC CAAAGATGGA TCAGTTTCAT
    TTGGATGATA ATGTCAAATC CCTCCTCTGT AGCTTGATCT GTTGTAGAAG TTCAATGACT
    GCCGAGCAGG TTTTGAATGC TGACTGTTTC TTGCTACCAA AGGGAAAATC AATGCCAAAC
    TCAGAGAAAG ATACTGAATA CATCCCCAAA AAAGAGGAAG AAGAATTAAA GGTGAAGAAC
    TTGGATAAAG ATAAGGAAAA GACAAGAAAT GGTGAAGCCA GCTTTGATTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015325207.1
    CDS251..3421
    Translation

    Target ORF information:

    RefSeq Version XM_015469721.2
    Organism Bos taurus(cattle)
    Definition Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015469721.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGGAGGAT TAACTGGCTT CAGCTCGCTG GCAGAAGGCT ACATACGATT AGATGACAGT 
    GGAATTGGTG CACCATCAGC TGAATTTTTA GACTCTCCAG TTATGGCCAT GGACCACCCA
    TTTCTAAAGG CATTTCAAGC ATCATCTAGC TCTTCTCCAG AAACGCTAAC AGGAATTGTC
    CAAATGGAGG AAGATAAACA TTGCAATAAA GTTGAAGATG TGGTTGGAAG TCATGTGGAA
    GATGCAGTAA CATTTTGGGC CCAGAGTATC AATAGAAATA AGGATATTAT GAAGATTGGT
    TGTTCACTGT CTGAAGTCTG TCCCCTTGCC AATTCAGTGT TTGGGAATCT TGATCCAAAG
    AAGATTTATG GTGGATTATT TTCTGAAGAT AAATGTTGGT ACAGATGCCA AGTACTGAAA
    GTCATCAACG ATGAAAAGTG TCTGGTGAGA TATATTGACT ATGGAAATAC AGAAATTCTG
    AATCGATCGG ATATAGTAGA GATTCCTTTG GATCTGCAAT TTTCTAGTGT TGCCAAAAAG
    TATAGACTTT GGGGACTACA GATTCCTTCT AGCCAAGAAG TTACCCAGTT TGATCAGGGC
    AGAACCTTTT TGGGGAGCTT GATTTTTGAA AAGGAAATTA AAATGAGAAT TAAAGCTACC
    TATCAAGATG GAACAATAAT TGCTCAGGCT GAGTATGGCA ATGTAGATAT TGGGGAAGAG
    GTGGCTAAGA AAGGATTTGC AGAAAAATGC AGACTCACTT CCAGCACTAA TATCTGTGAG
    GAAAAAAAAT CAGATCCTGG TCAGCTTGTC CTCAGGAACC TCAAAAGCCC CATTCCTTTG
    TGGGGGCATA GATCAAGCCA ATCAAACTTC ACCCGGCCAA AGGGGCGCTT AAGTGGGAGA
    ATGTGTCTTG ACTTGAAGAA TGAAAATGAT GCAGGAAATA ATGTGACATT TCCAAAGGAA
    AATTTGGCTG TTGGTGACTT TAATTTAGGG TCTAATGTCA GCCTGGCAAA AATTAAGCAG
    GACCAGAAAC TGATTGAAGA AAATGAAAAA CTTAAAACAG AGAAGGAGGT TCTTCTTGAA
    AGGTGCAAGG CATTAGAATT GAAAGTCGAA CAGACTGCCC AGGAGCTGCA GCAAGAGAAA
    GCAACTACCA TGGATTTGAC TAAACGTTTG GAAAGCACTG TGAAGACTTG TATAGGTACC
    AGAATGAAAA ATCTGGCAGC TAAGGTTGAA ATATTGAAAG AAATTAGACG TGTCAGTATC
    AGTGTTCGTT TTGGAAATGA CCTTTCAGAT GCTATACAAG TGTTGGATGA AGACTGCTTT
    ACTATCCCAG CTTCTTTGAA TGAATTAGAG CTAATTTGGG CAGAATATAA TCTGGCTCAG
    GAGAAGATTC AAACTTGTGA GAATGTGAAT GAAGGGAATA TTTTGATTGC TGAAAGAAAT
    GAAGTACAAC AGAAGCTGTA CATGGCAGTA GAAGTTTTTA TTCTGGAAGT TGATGAGTTA
    TCTCTTAATA AACGCTTAAA AACGTTGCAG GATTTGTCAG CCTCTTTAGA AGCAGTATAT
    GGACAGGCCA AAGAAGGAAT TAACTCTGAA GAAATACTGG AGAAATTTAA TGACTGGAGG
    TGTAATAAAA GAGAGGAGTT CACCAGTGTT AGAAGTGAAA CAGAGACTTG TATACAACGT
    CTTATAGCAT GGTTCCAGAA TACTTTAAAG GCCTTTGATC TGTCTGTGGA CAAATCACTG
    ATTTCTGATG ACACAATTGA TAATATTGAT GAAATCCTGG AGAACACTGA GTCAGGTGTC
    TGCAAGGAGC TGGAGATATC TCTGATTGAA CAAGGTGATG CAGACAAGGA GATCATTTTA
    AAAACATGTA GTCAAGTACT ACAAAAGATC CGTTCAGAGG AAAGCCTTAT TGCCACAGTA
    CAATCCAAGT ACAAGGACAG TGTTGAGTTT AAGAAACAGA TTATTGAATG TTTAAATAAG
    AGCCCCAGCG TGGATCACTT GCTTTCCATT AAAAAGACAC TGAAAAGCTT AAAGGCTCGA
    CTGAGATGGA AATTGGTTGA AAAGAATAAT TTGGAAGAAT CTGATGACCA TGATGGATCT
    GAGATTGAAA AAATAAAACA AGAAATAACT CAGTTACGGA ATAGTGTCTT TGAGGGAATT
    TATCATGAGC AGGAGGAATA TGCGAAGCTG AGTAGCTTAG TACAAAAATG GTTCCCTGAG
    CTTCCTCTAC TTCATCCTGA AATAGGATTA CTTAAGTACA TGAACTCTGG TGGTCTTCTT
    ACAATGAGCT TGGAAAGAGA TCTTCTTGAT GCTGAGCCTA TGAAGGAGCT TAGCAGCAAG
    CGTCCTTTGA TATGTTCTGA GGTTAATGGG CAGATAATTC TTTTAAAGGG CTATTCTGTG
    GATGTCGACA CAGAAGCCAG GGTAATTCAG AGAGCAGCTG CCTATCACAC AGCTTGGAGA
    GAAGCTAAAG GAGAATCTGG GTTGCTACCA CTGATATTCT TATTTTTGTG TAAGTCGGAT
    CCTATGGCTT ATCTGATGGT CCCATATTAT CCTAGGGCAA ATCTGAATGC TGTTCAGTCC
    ACCATGCCTT TAACTTCAGA AGAAGTTTTA AAGGTCATGA AAGGTGTAGC CCAGGGCCTT
    CATACATTGC ACAAGGCTGA CATAGTTCAT GGATCACTTC ATCAGAACAA TGTTTTTGCT
    TTAAACCGTC AACATGGAAT TGTTGGAGAT TTTGACTTCA CCAAATCTGT GAGTCAGCGA
    ACCTCAGTGA ACATGATGGT TGGTGACTTA AGTTTGCTAT CACCTGAACT GAAAATGGGA
    AAACCTGCTT CTCCGAGTTC TGACTTATAT GCTTATGGCT GCCTTTTATT GTGGCTTTCA
    GTTCAGAATC AGGAATTTGA AACTAATGAG GATGGAATCC CAAAGATGGA TCAGTTTCAT
    TTGGATGATA ATGTCAAATC CCTCCTCTGT AGCTTGATCT GTTGTAGAAG TTCAATGACT
    GCCGAGCAGG TTTTGAATGC TGACTGTTTC TTGCTACCAA AGGGAAAATC AATGCCAAAC
    TCAGAGAAAG ATACTGAATA CATCCCCAAA AAAGAGGAAG AAGAATTAAA GGTGAAGAAC
    TTGGATAAAG ATAAGGAAAA GACAAGAAAT GGTGAAGCCA GCTTTGATTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90626
    Clone ID Related Accession (Same CDS sequence) XM_015469721.1 , XM_015469721.2 , XM_015469719.1 , XM_015469719.2
    Accession Version XM_015469719.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3171bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein FAM221A isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103900.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGGAGGAT TAACTGGCTT CAGCTCGCTG GCAGAAGGCT ACATACGATT AGATGACAGT 
    GGAATTGGTG CACCATCAGC TGAATTTTTA GACTCTCCAG TTATGGCCAT GGACCACCCA
    TTTCTAAAGG CATTTCAAGC ATCATCTAGC TCTTCTCCAG AAACGCTAAC AGGAATTGTC
    CAAATGGAGG AAGATAAACA TTGCAATAAA GTTGAAGATG TGGTTGGAAG TCATGTGGAA
    GATGCAGTAA CATTTTGGGC CCAGAGTATC AATAGAAATA AGGATATTAT GAAGATTGGT
    TGTTCACTGT CTGAAGTCTG TCCCCTTGCC AATTCAGTGT TTGGGAATCT TGATCCAAAG
    AAGATTTATG GTGGATTATT TTCTGAAGAT AAATGTTGGT ACAGATGCCA AGTACTGAAA
    GTCATCAACG ATGAAAAGTG TCTGGTGAGA TATATTGACT ATGGAAATAC AGAAATTCTG
    AATCGATCGG ATATAGTAGA GATTCCTTTG GATCTGCAAT TTTCTAGTGT TGCCAAAAAG
    TATAGACTTT GGGGACTACA GATTCCTTCT AGCCAAGAAG TTACCCAGTT TGATCAGGGC
    AGAACCTTTT TGGGGAGCTT GATTTTTGAA AAGGAAATTA AAATGAGAAT TAAAGCTACC
    TATCAAGATG GAACAATAAT TGCTCAGGCT GAGTATGGCA ATGTAGATAT TGGGGAAGAG
    GTGGCTAAGA AAGGATTTGC AGAAAAATGC AGACTCACTT CCAGCACTAA TATCTGTGAG
    GAAAAAAAAT CAGATCCTGG TCAGCTTGTC CTCAGGAACC TCAAAAGCCC CATTCCTTTG
    TGGGGGCATA GATCAAGCCA ATCAAACTTC ACCCGGCCAA AGGGGCGCTT AAGTGGGAGA
    ATGTGTCTTG ACTTGAAGAA TGAAAATGAT GCAGGAAATA ATGTGACATT TCCAAAGGAA
    AATTTGGCTG TTGGTGACTT TAATTTAGGG TCTAATGTCA GCCTGGCAAA AATTAAGCAG
    GACCAGAAAC TGATTGAAGA AAATGAAAAA CTTAAAACAG AGAAGGAGGT TCTTCTTGAA
    AGGTGCAAGG CATTAGAATT GAAAGTCGAA CAGACTGCCC AGGAGCTGCA GCAAGAGAAA
    GCAACTACCA TGGATTTGAC TAAACGTTTG GAAAGCACTG TGAAGACTTG TATAGGTACC
    AGAATGAAAA ATCTGGCAGC TAAGGTTGAA ATATTGAAAG AAATTAGACG TGTCAGTATC
    AGTGTTCGTT TTGGAAATGA CCTTTCAGAT GCTATACAAG TGTTGGATGA AGACTGCTTT
    ACTATCCCAG CTTCTTTGAA TGAATTAGAG CTAATTTGGG CAGAATATAA TCTGGCTCAG
    GAGAAGATTC AAACTTGTGA GAATGTGAAT GAAGGGAATA TTTTGATTGC TGAAAGAAAT
    GAAGTACAAC AGAAGCTGTA CATGGCAGTA GAAGTTTTTA TTCTGGAAGT TGATGAGTTA
    TCTCTTAATA AACGCTTAAA AACGTTGCAG GATTTGTCAG CCTCTTTAGA AGCAGTATAT
    GGACAGGCCA AAGAAGGAAT TAACTCTGAA GAAATACTGG AGAAATTTAA TGACTGGAGG
    TGTAATAAAA GAGAGGAGTT CACCAGTGTT AGAAGTGAAA CAGAGACTTG TATACAACGT
    CTTATAGCAT GGTTCCAGAA TACTTTAAAG GCCTTTGATC TGTCTGTGGA CAAATCACTG
    ATTTCTGATG ACACAATTGA TAATATTGAT GAAATCCTGG AGAACACTGA GTCAGGTGTC
    TGCAAGGAGC TGGAGATATC TCTGATTGAA CAAGGTGATG CAGACAAGGA GATCATTTTA
    AAAACATGTA GTCAAGTACT ACAAAAGATC CGTTCAGAGG AAAGCCTTAT TGCCACAGTA
    CAATCCAAGT ACAAGGACAG TGTTGAGTTT AAGAAACAGA TTATTGAATG TTTAAATAAG
    AGCCCCAGCG TGGATCACTT GCTTTCCATT AAAAAGACAC TGAAAAGCTT AAAGGCTCGA
    CTGAGATGGA AATTGGTTGA AAAGAATAAT TTGGAAGAAT CTGATGACCA TGATGGATCT
    GAGATTGAAA AAATAAAACA AGAAATAACT CAGTTACGGA ATAGTGTCTT TGAGGGAATT
    TATCATGAGC AGGAGGAATA TGCGAAGCTG AGTAGCTTAG TACAAAAATG GTTCCCTGAG
    CTTCCTCTAC TTCATCCTGA AATAGGATTA CTTAAGTACA TGAACTCTGG TGGTCTTCTT
    ACAATGAGCT TGGAAAGAGA TCTTCTTGAT GCTGAGCCTA TGAAGGAGCT TAGCAGCAAG
    CGTCCTTTGA TATGTTCTGA GGTTAATGGG CAGATAATTC TTTTAAAGGG CTATTCTGTG
    GATGTCGACA CAGAAGCCAG GGTAATTCAG AGAGCAGCTG CCTATCACAC AGCTTGGAGA
    GAAGCTAAAG GAGAATCTGG GTTGCTACCA CTGATATTCT TATTTTTGTG TAAGTCGGAT
    CCTATGGCTT ATCTGATGGT CCCATATTAT CCTAGGGCAA ATCTGAATGC TGTTCAGTCC
    ACCATGCCTT TAACTTCAGA AGAAGTTTTA AAGGTCATGA AAGGTGTAGC CCAGGGCCTT
    CATACATTGC ACAAGGCTGA CATAGTTCAT GGATCACTTC ATCAGAACAA TGTTTTTGCT
    TTAAACCGTC AACATGGAAT TGTTGGAGAT TTTGACTTCA CCAAATCTGT GAGTCAGCGA
    ACCTCAGTGA ACATGATGGT TGGTGACTTA AGTTTGCTAT CACCTGAACT GAAAATGGGA
    AAACCTGCTT CTCCGAGTTC TGACTTATAT GCTTATGGCT GCCTTTTATT GTGGCTTTCA
    GTTCAGAATC AGGAATTTGA AACTAATGAG GATGGAATCC CAAAGATGGA TCAGTTTCAT
    TTGGATGATA ATGTCAAATC CCTCCTCTGT AGCTTGATCT GTTGTAGAAG TTCAATGACT
    GCCGAGCAGG TTTTGAATGC TGACTGTTTC TTGCTACCAA AGGGAAAATC AATGCCAAAC
    TCAGAGAAAG ATACTGAATA CATCCCCAAA AAAGAGGAAG AAGAATTAAA GGTGAAGAAC
    TTGGATAAAG ATAAGGAAAA GACAAGAAAT GGTGAAGCCA GCTTTGATTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015325205.1
    CDS493..3663
    Translation

    Target ORF information:

    RefSeq Version XM_015469719.1
    Organism Bos taurus(cattle)
    Definition Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015469719.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGGAGGAT TAACTGGCTT CAGCTCGCTG GCAGAAGGCT ACATACGATT AGATGACAGT 
    GGAATTGGTG CACCATCAGC TGAATTTTTA GACTCTCCAG TTATGGCCAT GGACCACCCA
    TTTCTAAAGG CATTTCAAGC ATCATCTAGC TCTTCTCCAG AAACGCTAAC AGGAATTGTC
    CAAATGGAGG AAGATAAACA TTGCAATAAA GTTGAAGATG TGGTTGGAAG TCATGTGGAA
    GATGCAGTAA CATTTTGGGC CCAGAGTATC AATAGAAATA AGGATATTAT GAAGATTGGT
    TGTTCACTGT CTGAAGTCTG TCCCCTTGCC AATTCAGTGT TTGGGAATCT TGATCCAAAG
    AAGATTTATG GTGGATTATT TTCTGAAGAT AAATGTTGGT ACAGATGCCA AGTACTGAAA
    GTCATCAACG ATGAAAAGTG TCTGGTGAGA TATATTGACT ATGGAAATAC AGAAATTCTG
    AATCGATCGG ATATAGTAGA GATTCCTTTG GATCTGCAAT TTTCTAGTGT TGCCAAAAAG
    TATAGACTTT GGGGACTACA GATTCCTTCT AGCCAAGAAG TTACCCAGTT TGATCAGGGC
    AGAACCTTTT TGGGGAGCTT GATTTTTGAA AAGGAAATTA AAATGAGAAT TAAAGCTACC
    TATCAAGATG GAACAATAAT TGCTCAGGCT GAGTATGGCA ATGTAGATAT TGGGGAAGAG
    GTGGCTAAGA AAGGATTTGC AGAAAAATGC AGACTCACTT CCAGCACTAA TATCTGTGAG
    GAAAAAAAAT CAGATCCTGG TCAGCTTGTC CTCAGGAACC TCAAAAGCCC CATTCCTTTG
    TGGGGGCATA GATCAAGCCA ATCAAACTTC ACCCGGCCAA AGGGGCGCTT AAGTGGGAGA
    ATGTGTCTTG ACTTGAAGAA TGAAAATGAT GCAGGAAATA ATGTGACATT TCCAAAGGAA
    AATTTGGCTG TTGGTGACTT TAATTTAGGG TCTAATGTCA GCCTGGCAAA AATTAAGCAG
    GACCAGAAAC TGATTGAAGA AAATGAAAAA CTTAAAACAG AGAAGGAGGT TCTTCTTGAA
    AGGTGCAAGG CATTAGAATT GAAAGTCGAA CAGACTGCCC AGGAGCTGCA GCAAGAGAAA
    GCAACTACCA TGGATTTGAC TAAACGTTTG GAAAGCACTG TGAAGACTTG TATAGGTACC
    AGAATGAAAA ATCTGGCAGC TAAGGTTGAA ATATTGAAAG AAATTAGACG TGTCAGTATC
    AGTGTTCGTT TTGGAAATGA CCTTTCAGAT GCTATACAAG TGTTGGATGA AGACTGCTTT
    ACTATCCCAG CTTCTTTGAA TGAATTAGAG CTAATTTGGG CAGAATATAA TCTGGCTCAG
    GAGAAGATTC AAACTTGTGA GAATGTGAAT GAAGGGAATA TTTTGATTGC TGAAAGAAAT
    GAAGTACAAC AGAAGCTGTA CATGGCAGTA GAAGTTTTTA TTCTGGAAGT TGATGAGTTA
    TCTCTTAATA AACGCTTAAA AACGTTGCAG GATTTGTCAG CCTCTTTAGA AGCAGTATAT
    GGACAGGCCA AAGAAGGAAT TAACTCTGAA GAAATACTGG AGAAATTTAA TGACTGGAGG
    TGTAATAAAA GAGAGGAGTT CACCAGTGTT AGAAGTGAAA CAGAGACTTG TATACAACGT
    CTTATAGCAT GGTTCCAGAA TACTTTAAAG GCCTTTGATC TGTCTGTGGA CAAATCACTG
    ATTTCTGATG ACACAATTGA TAATATTGAT GAAATCCTGG AGAACACTGA GTCAGGTGTC
    TGCAAGGAGC TGGAGATATC TCTGATTGAA CAAGGTGATG CAGACAAGGA GATCATTTTA
    AAAACATGTA GTCAAGTACT ACAAAAGATC CGTTCAGAGG AAAGCCTTAT TGCCACAGTA
    CAATCCAAGT ACAAGGACAG TGTTGAGTTT AAGAAACAGA TTATTGAATG TTTAAATAAG
    AGCCCCAGCG TGGATCACTT GCTTTCCATT AAAAAGACAC TGAAAAGCTT AAAGGCTCGA
    CTGAGATGGA AATTGGTTGA AAAGAATAAT TTGGAAGAAT CTGATGACCA TGATGGATCT
    GAGATTGAAA AAATAAAACA AGAAATAACT CAGTTACGGA ATAGTGTCTT TGAGGGAATT
    TATCATGAGC AGGAGGAATA TGCGAAGCTG AGTAGCTTAG TACAAAAATG GTTCCCTGAG
    CTTCCTCTAC TTCATCCTGA AATAGGATTA CTTAAGTACA TGAACTCTGG TGGTCTTCTT
    ACAATGAGCT TGGAAAGAGA TCTTCTTGAT GCTGAGCCTA TGAAGGAGCT TAGCAGCAAG
    CGTCCTTTGA TATGTTCTGA GGTTAATGGG CAGATAATTC TTTTAAAGGG CTATTCTGTG
    GATGTCGACA CAGAAGCCAG GGTAATTCAG AGAGCAGCTG CCTATCACAC AGCTTGGAGA
    GAAGCTAAAG GAGAATCTGG GTTGCTACCA CTGATATTCT TATTTTTGTG TAAGTCGGAT
    CCTATGGCTT ATCTGATGGT CCCATATTAT CCTAGGGCAA ATCTGAATGC TGTTCAGTCC
    ACCATGCCTT TAACTTCAGA AGAAGTTTTA AAGGTCATGA AAGGTGTAGC CCAGGGCCTT
    CATACATTGC ACAAGGCTGA CATAGTTCAT GGATCACTTC ATCAGAACAA TGTTTTTGCT
    TTAAACCGTC AACATGGAAT TGTTGGAGAT TTTGACTTCA CCAAATCTGT GAGTCAGCGA
    ACCTCAGTGA ACATGATGGT TGGTGACTTA AGTTTGCTAT CACCTGAACT GAAAATGGGA
    AAACCTGCTT CTCCGAGTTC TGACTTATAT GCTTATGGCT GCCTTTTATT GTGGCTTTCA
    GTTCAGAATC AGGAATTTGA AACTAATGAG GATGGAATCC CAAAGATGGA TCAGTTTCAT
    TTGGATGATA ATGTCAAATC CCTCCTCTGT AGCTTGATCT GTTGTAGAAG TTCAATGACT
    GCCGAGCAGG TTTTGAATGC TGACTGTTTC TTGCTACCAA AGGGAAAATC AATGCCAAAC
    TCAGAGAAAG ATACTGAATA CATCCCCAAA AAAGAGGAAG AAGAATTAAA GGTGAAGAAC
    TTGGATAAAG ATAAGGAAAA GACAAGAAAT GGTGAAGCCA GCTTTGATTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90626
    Clone ID Related Accession (Same CDS sequence) XM_015469721.1 , XM_015469721.2 , XM_015469719.1 , XM_015469719.2
    Accession Version XM_015469719.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3171bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein FAM221A isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015469719.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGGAGGAT TAACTGGCTT CAGCTCGCTG GCAGAAGGCT ACATACGATT AGATGACAGT 
    GGAATTGGTG CACCATCAGC TGAATTTTTA GACTCTCCAG TTATGGCCAT GGACCACCCA
    TTTCTAAAGG CATTTCAAGC ATCATCTAGC TCTTCTCCAG AAACGCTAAC AGGAATTGTC
    CAAATGGAGG AAGATAAACA TTGCAATAAA GTTGAAGATG TGGTTGGAAG TCATGTGGAA
    GATGCAGTAA CATTTTGGGC CCAGAGTATC AATAGAAATA AGGATATTAT GAAGATTGGT
    TGTTCACTGT CTGAAGTCTG TCCCCTTGCC AATTCAGTGT TTGGGAATCT TGATCCAAAG
    AAGATTTATG GTGGATTATT TTCTGAAGAT AAATGTTGGT ACAGATGCCA AGTACTGAAA
    GTCATCAACG ATGAAAAGTG TCTGGTGAGA TATATTGACT ATGGAAATAC AGAAATTCTG
    AATCGATCGG ATATAGTAGA GATTCCTTTG GATCTGCAAT TTTCTAGTGT TGCCAAAAAG
    TATAGACTTT GGGGACTACA GATTCCTTCT AGCCAAGAAG TTACCCAGTT TGATCAGGGC
    AGAACCTTTT TGGGGAGCTT GATTTTTGAA AAGGAAATTA AAATGAGAAT TAAAGCTACC
    TATCAAGATG GAACAATAAT TGCTCAGGCT GAGTATGGCA ATGTAGATAT TGGGGAAGAG
    GTGGCTAAGA AAGGATTTGC AGAAAAATGC AGACTCACTT CCAGCACTAA TATCTGTGAG
    GAAAAAAAAT CAGATCCTGG TCAGCTTGTC CTCAGGAACC TCAAAAGCCC CATTCCTTTG
    TGGGGGCATA GATCAAGCCA ATCAAACTTC ACCCGGCCAA AGGGGCGCTT AAGTGGGAGA
    ATGTGTCTTG ACTTGAAGAA TGAAAATGAT GCAGGAAATA ATGTGACATT TCCAAAGGAA
    AATTTGGCTG TTGGTGACTT TAATTTAGGG TCTAATGTCA GCCTGGCAAA AATTAAGCAG
    GACCAGAAAC TGATTGAAGA AAATGAAAAA CTTAAAACAG AGAAGGAGGT TCTTCTTGAA
    AGGTGCAAGG CATTAGAATT GAAAGTCGAA CAGACTGCCC AGGAGCTGCA GCAAGAGAAA
    GCAACTACCA TGGATTTGAC TAAACGTTTG GAAAGCACTG TGAAGACTTG TATAGGTACC
    AGAATGAAAA ATCTGGCAGC TAAGGTTGAA ATATTGAAAG AAATTAGACG TGTCAGTATC
    AGTGTTCGTT TTGGAAATGA CCTTTCAGAT GCTATACAAG TGTTGGATGA AGACTGCTTT
    ACTATCCCAG CTTCTTTGAA TGAATTAGAG CTAATTTGGG CAGAATATAA TCTGGCTCAG
    GAGAAGATTC AAACTTGTGA GAATGTGAAT GAAGGGAATA TTTTGATTGC TGAAAGAAAT
    GAAGTACAAC AGAAGCTGTA CATGGCAGTA GAAGTTTTTA TTCTGGAAGT TGATGAGTTA
    TCTCTTAATA AACGCTTAAA AACGTTGCAG GATTTGTCAG CCTCTTTAGA AGCAGTATAT
    GGACAGGCCA AAGAAGGAAT TAACTCTGAA GAAATACTGG AGAAATTTAA TGACTGGAGG
    TGTAATAAAA GAGAGGAGTT CACCAGTGTT AGAAGTGAAA CAGAGACTTG TATACAACGT
    CTTATAGCAT GGTTCCAGAA TACTTTAAAG GCCTTTGATC TGTCTGTGGA CAAATCACTG
    ATTTCTGATG ACACAATTGA TAATATTGAT GAAATCCTGG AGAACACTGA GTCAGGTGTC
    TGCAAGGAGC TGGAGATATC TCTGATTGAA CAAGGTGATG CAGACAAGGA GATCATTTTA
    AAAACATGTA GTCAAGTACT ACAAAAGATC CGTTCAGAGG AAAGCCTTAT TGCCACAGTA
    CAATCCAAGT ACAAGGACAG TGTTGAGTTT AAGAAACAGA TTATTGAATG TTTAAATAAG
    AGCCCCAGCG TGGATCACTT GCTTTCCATT AAAAAGACAC TGAAAAGCTT AAAGGCTCGA
    CTGAGATGGA AATTGGTTGA AAAGAATAAT TTGGAAGAAT CTGATGACCA TGATGGATCT
    GAGATTGAAA AAATAAAACA AGAAATAACT CAGTTACGGA ATAGTGTCTT TGAGGGAATT
    TATCATGAGC AGGAGGAATA TGCGAAGCTG AGTAGCTTAG TACAAAAATG GTTCCCTGAG
    CTTCCTCTAC TTCATCCTGA AATAGGATTA CTTAAGTACA TGAACTCTGG TGGTCTTCTT
    ACAATGAGCT TGGAAAGAGA TCTTCTTGAT GCTGAGCCTA TGAAGGAGCT TAGCAGCAAG
    CGTCCTTTGA TATGTTCTGA GGTTAATGGG CAGATAATTC TTTTAAAGGG CTATTCTGTG
    GATGTCGACA CAGAAGCCAG GGTAATTCAG AGAGCAGCTG CCTATCACAC AGCTTGGAGA
    GAAGCTAAAG GAGAATCTGG GTTGCTACCA CTGATATTCT TATTTTTGTG TAAGTCGGAT
    CCTATGGCTT ATCTGATGGT CCCATATTAT CCTAGGGCAA ATCTGAATGC TGTTCAGTCC
    ACCATGCCTT TAACTTCAGA AGAAGTTTTA AAGGTCATGA AAGGTGTAGC CCAGGGCCTT
    CATACATTGC ACAAGGCTGA CATAGTTCAT GGATCACTTC ATCAGAACAA TGTTTTTGCT
    TTAAACCGTC AACATGGAAT TGTTGGAGAT TTTGACTTCA CCAAATCTGT GAGTCAGCGA
    ACCTCAGTGA ACATGATGGT TGGTGACTTA AGTTTGCTAT CACCTGAACT GAAAATGGGA
    AAACCTGCTT CTCCGAGTTC TGACTTATAT GCTTATGGCT GCCTTTTATT GTGGCTTTCA
    GTTCAGAATC AGGAATTTGA AACTAATGAG GATGGAATCC CAAAGATGGA TCAGTTTCAT
    TTGGATGATA ATGTCAAATC CCTCCTCTGT AGCTTGATCT GTTGTAGAAG TTCAATGACT
    GCCGAGCAGG TTTTGAATGC TGACTGTTTC TTGCTACCAA AGGGAAAATC AATGCCAAAC
    TCAGAGAAAG ATACTGAATA CATCCCCAAA AAAGAGGAAG AAGAATTAAA GGTGAAGAAC
    TTGGATAAAG ATAAGGAAAA GACAAGAAAT GGTGAAGCCA GCTTTGATTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015325205.1
    CDS629..3799
    Translation

    Target ORF information:

    RefSeq Version XM_015469719.2
    Organism Bos taurus(cattle)
    Definition Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015469719.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGGAGGAT TAACTGGCTT CAGCTCGCTG GCAGAAGGCT ACATACGATT AGATGACAGT 
    GGAATTGGTG CACCATCAGC TGAATTTTTA GACTCTCCAG TTATGGCCAT GGACCACCCA
    TTTCTAAAGG CATTTCAAGC ATCATCTAGC TCTTCTCCAG AAACGCTAAC AGGAATTGTC
    CAAATGGAGG AAGATAAACA TTGCAATAAA GTTGAAGATG TGGTTGGAAG TCATGTGGAA
    GATGCAGTAA CATTTTGGGC CCAGAGTATC AATAGAAATA AGGATATTAT GAAGATTGGT
    TGTTCACTGT CTGAAGTCTG TCCCCTTGCC AATTCAGTGT TTGGGAATCT TGATCCAAAG
    AAGATTTATG GTGGATTATT TTCTGAAGAT AAATGTTGGT ACAGATGCCA AGTACTGAAA
    GTCATCAACG ATGAAAAGTG TCTGGTGAGA TATATTGACT ATGGAAATAC AGAAATTCTG
    AATCGATCGG ATATAGTAGA GATTCCTTTG GATCTGCAAT TTTCTAGTGT TGCCAAAAAG
    TATAGACTTT GGGGACTACA GATTCCTTCT AGCCAAGAAG TTACCCAGTT TGATCAGGGC
    AGAACCTTTT TGGGGAGCTT GATTTTTGAA AAGGAAATTA AAATGAGAAT TAAAGCTACC
    TATCAAGATG GAACAATAAT TGCTCAGGCT GAGTATGGCA ATGTAGATAT TGGGGAAGAG
    GTGGCTAAGA AAGGATTTGC AGAAAAATGC AGACTCACTT CCAGCACTAA TATCTGTGAG
    GAAAAAAAAT CAGATCCTGG TCAGCTTGTC CTCAGGAACC TCAAAAGCCC CATTCCTTTG
    TGGGGGCATA GATCAAGCCA ATCAAACTTC ACCCGGCCAA AGGGGCGCTT AAGTGGGAGA
    ATGTGTCTTG ACTTGAAGAA TGAAAATGAT GCAGGAAATA ATGTGACATT TCCAAAGGAA
    AATTTGGCTG TTGGTGACTT TAATTTAGGG TCTAATGTCA GCCTGGCAAA AATTAAGCAG
    GACCAGAAAC TGATTGAAGA AAATGAAAAA CTTAAAACAG AGAAGGAGGT TCTTCTTGAA
    AGGTGCAAGG CATTAGAATT GAAAGTCGAA CAGACTGCCC AGGAGCTGCA GCAAGAGAAA
    GCAACTACCA TGGATTTGAC TAAACGTTTG GAAAGCACTG TGAAGACTTG TATAGGTACC
    AGAATGAAAA ATCTGGCAGC TAAGGTTGAA ATATTGAAAG AAATTAGACG TGTCAGTATC
    AGTGTTCGTT TTGGAAATGA CCTTTCAGAT GCTATACAAG TGTTGGATGA AGACTGCTTT
    ACTATCCCAG CTTCTTTGAA TGAATTAGAG CTAATTTGGG CAGAATATAA TCTGGCTCAG
    GAGAAGATTC AAACTTGTGA GAATGTGAAT GAAGGGAATA TTTTGATTGC TGAAAGAAAT
    GAAGTACAAC AGAAGCTGTA CATGGCAGTA GAAGTTTTTA TTCTGGAAGT TGATGAGTTA
    TCTCTTAATA AACGCTTAAA AACGTTGCAG GATTTGTCAG CCTCTTTAGA AGCAGTATAT
    GGACAGGCCA AAGAAGGAAT TAACTCTGAA GAAATACTGG AGAAATTTAA TGACTGGAGG
    TGTAATAAAA GAGAGGAGTT CACCAGTGTT AGAAGTGAAA CAGAGACTTG TATACAACGT
    CTTATAGCAT GGTTCCAGAA TACTTTAAAG GCCTTTGATC TGTCTGTGGA CAAATCACTG
    ATTTCTGATG ACACAATTGA TAATATTGAT GAAATCCTGG AGAACACTGA GTCAGGTGTC
    TGCAAGGAGC TGGAGATATC TCTGATTGAA CAAGGTGATG CAGACAAGGA GATCATTTTA
    AAAACATGTA GTCAAGTACT ACAAAAGATC CGTTCAGAGG AAAGCCTTAT TGCCACAGTA
    CAATCCAAGT ACAAGGACAG TGTTGAGTTT AAGAAACAGA TTATTGAATG TTTAAATAAG
    AGCCCCAGCG TGGATCACTT GCTTTCCATT AAAAAGACAC TGAAAAGCTT AAAGGCTCGA
    CTGAGATGGA AATTGGTTGA AAAGAATAAT TTGGAAGAAT CTGATGACCA TGATGGATCT
    GAGATTGAAA AAATAAAACA AGAAATAACT CAGTTACGGA ATAGTGTCTT TGAGGGAATT
    TATCATGAGC AGGAGGAATA TGCGAAGCTG AGTAGCTTAG TACAAAAATG GTTCCCTGAG
    CTTCCTCTAC TTCATCCTGA AATAGGATTA CTTAAGTACA TGAACTCTGG TGGTCTTCTT
    ACAATGAGCT TGGAAAGAGA TCTTCTTGAT GCTGAGCCTA TGAAGGAGCT TAGCAGCAAG
    CGTCCTTTGA TATGTTCTGA GGTTAATGGG CAGATAATTC TTTTAAAGGG CTATTCTGTG
    GATGTCGACA CAGAAGCCAG GGTAATTCAG AGAGCAGCTG CCTATCACAC AGCTTGGAGA
    GAAGCTAAAG GAGAATCTGG GTTGCTACCA CTGATATTCT TATTTTTGTG TAAGTCGGAT
    CCTATGGCTT ATCTGATGGT CCCATATTAT CCTAGGGCAA ATCTGAATGC TGTTCAGTCC
    ACCATGCCTT TAACTTCAGA AGAAGTTTTA AAGGTCATGA AAGGTGTAGC CCAGGGCCTT
    CATACATTGC ACAAGGCTGA CATAGTTCAT GGATCACTTC ATCAGAACAA TGTTTTTGCT
    TTAAACCGTC AACATGGAAT TGTTGGAGAT TTTGACTTCA CCAAATCTGT GAGTCAGCGA
    ACCTCAGTGA ACATGATGGT TGGTGACTTA AGTTTGCTAT CACCTGAACT GAAAATGGGA
    AAACCTGCTT CTCCGAGTTC TGACTTATAT GCTTATGGCT GCCTTTTATT GTGGCTTTCA
    GTTCAGAATC AGGAATTTGA AACTAATGAG GATGGAATCC CAAAGATGGA TCAGTTTCAT
    TTGGATGATA ATGTCAAATC CCTCCTCTGT AGCTTGATCT GTTGTAGAAG TTCAATGACT
    GCCGAGCAGG TTTTGAATGC TGACTGTTTC TTGCTACCAA AGGGAAAATC AATGCCAAAC
    TCAGAGAAAG ATACTGAATA CATCCCCAAA AAAGAGGAAG AAGAATTAAA GGTGAAGAAC
    TTGGATAAAG ATAAGGAAAA GACAAGAAAT GGTGAAGCCA GCTTTGATTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90627
    Clone ID Related Accession (Same CDS sequence) XM_015469725.1 , XM_015469729.1 , XM_024990536.1 , XM_015469725.2
    Accession Version XM_015469725.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3069bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein FAM221A isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103900.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCCATGG ACCACCCATT TCTAAAGGCA TTTCAAGCAT CATCTAGCTC TTCTCCAGAA 
    ACGCTAACAG GAATTGTCCA AATGGAGGAA GATAAACATT GCAATAAAGT TGAAGATGTG
    GTTGGAAGTC ATGTGGAAGA TGCAGTAACA TTTTGGGCCC AGAGTATCAA TAGAAATAAG
    GATATTATGA AGATTGGTTG TTCACTGTCT GAAGTCTGTC CCCTTGCCAA TTCAGTGTTT
    GGGAATCTTG ATCCAAAGAA GATTTATGGT GGATTATTTT CTGAAGATAA ATGTTGGTAC
    AGATGCCAAG TACTGAAAGT CATCAACGAT GAAAAGTGTC TGGTGAGATA TATTGACTAT
    GGAAATACAG AAATTCTGAA TCGATCGGAT ATAGTAGAGA TTCCTTTGGA TCTGCAATTT
    TCTAGTGTTG CCAAAAAGTA TAGACTTTGG GGACTACAGA TTCCTTCTAG CCAAGAAGTT
    ACCCAGTTTG ATCAGGGCAG AACCTTTTTG GGGAGCTTGA TTTTTGAAAA GGAAATTAAA
    ATGAGAATTA AAGCTACCTA TCAAGATGGA ACAATAATTG CTCAGGCTGA GTATGGCAAT
    GTAGATATTG GGGAAGAGGT GGCTAAGAAA GGATTTGCAG AAAAATGCAG ACTCACTTCC
    AGCACTAATA TCTGTGAGGA AAAAAAATCA GATCCTGGTC AGCTTGTCCT CAGGAACCTC
    AAAAGCCCCA TTCCTTTGTG GGGGCATAGA TCAAGCCAAT CAAACTTCAC CCGGCCAAAG
    GGGCGCTTAA GTGGGAGAAT GTGTCTTGAC TTGAAGAATG AAAATGATGC AGGAAATAAT
    GTGACATTTC CAAAGGAAAA TTTGGCTGTT GGTGACTTTA ATTTAGGGTC TAATGTCAGC
    CTGGCAAAAA TTAAGCAGGA CCAGAAACTG ATTGAAGAAA ATGAAAAACT TAAAACAGAG
    AAGGAGGTTC TTCTTGAAAG GTGCAAGGCA TTAGAATTGA AAGTCGAACA GACTGCCCAG
    GAGCTGCAGC AAGAGAAAGC AACTACCATG GATTTGACTA AACGTTTGGA AAGCACTGTG
    AAGACTTGTA TAGGTACCAG AATGAAAAAT CTGGCAGCTA AGGTTGAAAT ATTGAAAGAA
    ATTAGACGTG TCAGTATCAG TGTTCGTTTT GGAAATGACC TTTCAGATGC TATACAAGTG
    TTGGATGAAG ACTGCTTTAC TATCCCAGCT TCTTTGAATG AATTAGAGCT AATTTGGGCA
    GAATATAATC TGGCTCAGGA GAAGATTCAA ACTTGTGAGA ATGTGAATGA AGGGAATATT
    TTGATTGCTG AAAGAAATGA AGTACAACAG AAGCTGTACA TGGCAGTAGA AGTTTTTATT
    CTGGAAGTTG ATGAGTTATC TCTTAATAAA CGCTTAAAAA CGTTGCAGGA TTTGTCAGCC
    TCTTTAGAAG CAGTATATGG ACAGGCCAAA GAAGGAATTA ACTCTGAAGA AATACTGGAG
    AAATTTAATG ACTGGAGGTG TAATAAAAGA GAGGAGTTCA CCAGTGTTAG AAGTGAAACA
    GAGACTTGTA TACAACGTCT TATAGCATGG TTCCAGAATA CTTTAAAGGC CTTTGATCTG
    TCTGTGGACA AATCACTGAT TTCTGATGAC ACAATTGATA ATATTGATGA AATCCTGGAG
    AACACTGAGT CAGGTGTCTG CAAGGAGCTG GAGATATCTC TGATTGAACA AGGTGATGCA
    GACAAGGAGA TCATTTTAAA AACATGTAGT CAAGTACTAC AAAAGATCCG TTCAGAGGAA
    AGCCTTATTG CCACAGTACA ATCCAAGTAC AAGGACAGTG TTGAGTTTAA GAAACAGATT
    ATTGAATGTT TAAATAAGAG CCCCAGCGTG GATCACTTGC TTTCCATTAA AAAGACACTG
    AAAAGCTTAA AGGCTCGACT GAGATGGAAA TTGGTTGAAA AGAATAATTT GGAAGAATCT
    GATGACCATG ATGGATCTGA GATTGAAAAA ATAAAACAAG AAATAACTCA GTTACGGAAT
    AGTGTCTTTG AGGGAATTTA TCATGAGCAG GAGGAATATG CGAAGCTGAG TAGCTTAGTA
    CAAAAATGGT TCCCTGAGCT TCCTCTACTT CATCCTGAAA TAGGATTACT TAAGTACATG
    AACTCTGGTG GTCTTCTTAC AATGAGCTTG GAAAGAGATC TTCTTGATGC TGAGCCTATG
    AAGGAGCTTA GCAGCAAGCG TCCTTTGATA TGTTCTGAGG TTAATGGGCA GATAATTCTT
    TTAAAGGGCT ATTCTGTGGA TGTCGACACA GAAGCCAGGG TAATTCAGAG AGCAGCTGCC
    TATCACACAG CTTGGAGAGA AGCTAAAGGA GAATCTGGGT TGCTACCACT GATATTCTTA
    TTTTTGTGTA AGTCGGATCC TATGGCTTAT CTGATGGTCC CATATTATCC TAGGGCAAAT
    CTGAATGCTG TTCAGTCCAC CATGCCTTTA ACTTCAGAAG AAGTTTTAAA GGTCATGAAA
    GGTGTAGCCC AGGGCCTTCA TACATTGCAC AAGGCTGACA TAGTTCATGG ATCACTTCAT
    CAGAACAATG TTTTTGCTTT AAACCGTCAA CATGGAATTG TTGGAGATTT TGACTTCACC
    AAATCTGTGA GTCAGCGAAC CTCAGTGAAC ATGATGGTTG GTGACTTAAG TTTGCTATCA
    CCTGAACTGA AAATGGGAAA ACCTGCTTCT CCGAGTTCTG ACTTATATGC TTATGGCTGC
    CTTTTATTGT GGCTTTCAGT TCAGAATCAG GAATTTGAAA CTAATGAGGA TGGAATCCCA
    AAGATGGATC AGTTTCATTT GGATGATAAT GTCAAATCCC TCCTCTGTAG CTTGATCTGT
    TGTAGAAGTT CAATGACTGC CGAGCAGGTT TTGAATGCTG ACTGTTTCTT GCTACCAAAG
    GGAAAATCAA TGCCAAACTC AGAGAAAGAT ACTGAATACA TCCCCAAAAA AGAGGAAGAA
    GAATTAAAGG TGAAGAACTT GGATAAAGAT AAGGAAAAGA CAAGAAATGG TGAAGCCAGC
    TTTGATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015325211.1
    CDS157..3225
    Translation

    Target ORF information:

    RefSeq Version XM_015469725.1
    Organism Bos taurus(cattle)
    Definition Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015469725.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCCATGG ACCACCCATT TCTAAAGGCA TTTCAAGCAT CATCTAGCTC TTCTCCAGAA 
    ACGCTAACAG GAATTGTCCA AATGGAGGAA GATAAACATT GCAATAAAGT TGAAGATGTG
    GTTGGAAGTC ATGTGGAAGA TGCAGTAACA TTTTGGGCCC AGAGTATCAA TAGAAATAAG
    GATATTATGA AGATTGGTTG TTCACTGTCT GAAGTCTGTC CCCTTGCCAA TTCAGTGTTT
    GGGAATCTTG ATCCAAAGAA GATTTATGGT GGATTATTTT CTGAAGATAA ATGTTGGTAC
    AGATGCCAAG TACTGAAAGT CATCAACGAT GAAAAGTGTC TGGTGAGATA TATTGACTAT
    GGAAATACAG AAATTCTGAA TCGATCGGAT ATAGTAGAGA TTCCTTTGGA TCTGCAATTT
    TCTAGTGTTG CCAAAAAGTA TAGACTTTGG GGACTACAGA TTCCTTCTAG CCAAGAAGTT
    ACCCAGTTTG ATCAGGGCAG AACCTTTTTG GGGAGCTTGA TTTTTGAAAA GGAAATTAAA
    ATGAGAATTA AAGCTACCTA TCAAGATGGA ACAATAATTG CTCAGGCTGA GTATGGCAAT
    GTAGATATTG GGGAAGAGGT GGCTAAGAAA GGATTTGCAG AAAAATGCAG ACTCACTTCC
    AGCACTAATA TCTGTGAGGA AAAAAAATCA GATCCTGGTC AGCTTGTCCT CAGGAACCTC
    AAAAGCCCCA TTCCTTTGTG GGGGCATAGA TCAAGCCAAT CAAACTTCAC CCGGCCAAAG
    GGGCGCTTAA GTGGGAGAAT GTGTCTTGAC TTGAAGAATG AAAATGATGC AGGAAATAAT
    GTGACATTTC CAAAGGAAAA TTTGGCTGTT GGTGACTTTA ATTTAGGGTC TAATGTCAGC
    CTGGCAAAAA TTAAGCAGGA CCAGAAACTG ATTGAAGAAA ATGAAAAACT TAAAACAGAG
    AAGGAGGTTC TTCTTGAAAG GTGCAAGGCA TTAGAATTGA AAGTCGAACA GACTGCCCAG
    GAGCTGCAGC AAGAGAAAGC AACTACCATG GATTTGACTA AACGTTTGGA AAGCACTGTG
    AAGACTTGTA TAGGTACCAG AATGAAAAAT CTGGCAGCTA AGGTTGAAAT ATTGAAAGAA
    ATTAGACGTG TCAGTATCAG TGTTCGTTTT GGAAATGACC TTTCAGATGC TATACAAGTG
    TTGGATGAAG ACTGCTTTAC TATCCCAGCT TCTTTGAATG AATTAGAGCT AATTTGGGCA
    GAATATAATC TGGCTCAGGA GAAGATTCAA ACTTGTGAGA ATGTGAATGA AGGGAATATT
    TTGATTGCTG AAAGAAATGA AGTACAACAG AAGCTGTACA TGGCAGTAGA AGTTTTTATT
    CTGGAAGTTG ATGAGTTATC TCTTAATAAA CGCTTAAAAA CGTTGCAGGA TTTGTCAGCC
    TCTTTAGAAG CAGTATATGG ACAGGCCAAA GAAGGAATTA ACTCTGAAGA AATACTGGAG
    AAATTTAATG ACTGGAGGTG TAATAAAAGA GAGGAGTTCA CCAGTGTTAG AAGTGAAACA
    GAGACTTGTA TACAACGTCT TATAGCATGG TTCCAGAATA CTTTAAAGGC CTTTGATCTG
    TCTGTGGACA AATCACTGAT TTCTGATGAC ACAATTGATA ATATTGATGA AATCCTGGAG
    AACACTGAGT CAGGTGTCTG CAAGGAGCTG GAGATATCTC TGATTGAACA AGGTGATGCA
    GACAAGGAGA TCATTTTAAA AACATGTAGT CAAGTACTAC AAAAGATCCG TTCAGAGGAA
    AGCCTTATTG CCACAGTACA ATCCAAGTAC AAGGACAGTG TTGAGTTTAA GAAACAGATT
    ATTGAATGTT TAAATAAGAG CCCCAGCGTG GATCACTTGC TTTCCATTAA AAAGACACTG
    AAAAGCTTAA AGGCTCGACT GAGATGGAAA TTGGTTGAAA AGAATAATTT GGAAGAATCT
    GATGACCATG ATGGATCTGA GATTGAAAAA ATAAAACAAG AAATAACTCA GTTACGGAAT
    AGTGTCTTTG AGGGAATTTA TCATGAGCAG GAGGAATATG CGAAGCTGAG TAGCTTAGTA
    CAAAAATGGT TCCCTGAGCT TCCTCTACTT CATCCTGAAA TAGGATTACT TAAGTACATG
    AACTCTGGTG GTCTTCTTAC AATGAGCTTG GAAAGAGATC TTCTTGATGC TGAGCCTATG
    AAGGAGCTTA GCAGCAAGCG TCCTTTGATA TGTTCTGAGG TTAATGGGCA GATAATTCTT
    TTAAAGGGCT ATTCTGTGGA TGTCGACACA GAAGCCAGGG TAATTCAGAG AGCAGCTGCC
    TATCACACAG CTTGGAGAGA AGCTAAAGGA GAATCTGGGT TGCTACCACT GATATTCTTA
    TTTTTGTGTA AGTCGGATCC TATGGCTTAT CTGATGGTCC CATATTATCC TAGGGCAAAT
    CTGAATGCTG TTCAGTCCAC CATGCCTTTA ACTTCAGAAG AAGTTTTAAA GGTCATGAAA
    GGTGTAGCCC AGGGCCTTCA TACATTGCAC AAGGCTGACA TAGTTCATGG ATCACTTCAT
    CAGAACAATG TTTTTGCTTT AAACCGTCAA CATGGAATTG TTGGAGATTT TGACTTCACC
    AAATCTGTGA GTCAGCGAAC CTCAGTGAAC ATGATGGTTG GTGACTTAAG TTTGCTATCA
    CCTGAACTGA AAATGGGAAA ACCTGCTTCT CCGAGTTCTG ACTTATATGC TTATGGCTGC
    CTTTTATTGT GGCTTTCAGT TCAGAATCAG GAATTTGAAA CTAATGAGGA TGGAATCCCA
    AAGATGGATC AGTTTCATTT GGATGATAAT GTCAAATCCC TCCTCTGTAG CTTGATCTGT
    TGTAGAAGTT CAATGACTGC CGAGCAGGTT TTGAATGCTG ACTGTTTCTT GCTACCAAAG
    GGAAAATCAA TGCCAAACTC AGAGAAAGAT ACTGAATACA TCCCCAAAAA AGAGGAAGAA
    GAATTAAAGG TGAAGAACTT GGATAAAGAT AAGGAAAAGA CAAGAAATGG TGAAGCCAGC
    TTTGATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90627
    Clone ID Related Accession (Same CDS sequence) XM_015469725.1 , XM_015469729.1 , XM_024990536.1 , XM_015469725.2
    Accession Version XM_015469729.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3069bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein FAM221A isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103900.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCCATGG ACCACCCATT TCTAAAGGCA TTTCAAGCAT CATCTAGCTC TTCTCCAGAA 
    ACGCTAACAG GAATTGTCCA AATGGAGGAA GATAAACATT GCAATAAAGT TGAAGATGTG
    GTTGGAAGTC ATGTGGAAGA TGCAGTAACA TTTTGGGCCC AGAGTATCAA TAGAAATAAG
    GATATTATGA AGATTGGTTG TTCACTGTCT GAAGTCTGTC CCCTTGCCAA TTCAGTGTTT
    GGGAATCTTG ATCCAAAGAA GATTTATGGT GGATTATTTT CTGAAGATAA ATGTTGGTAC
    AGATGCCAAG TACTGAAAGT CATCAACGAT GAAAAGTGTC TGGTGAGATA TATTGACTAT
    GGAAATACAG AAATTCTGAA TCGATCGGAT ATAGTAGAGA TTCCTTTGGA TCTGCAATTT
    TCTAGTGTTG CCAAAAAGTA TAGACTTTGG GGACTACAGA TTCCTTCTAG CCAAGAAGTT
    ACCCAGTTTG ATCAGGGCAG AACCTTTTTG GGGAGCTTGA TTTTTGAAAA GGAAATTAAA
    ATGAGAATTA AAGCTACCTA TCAAGATGGA ACAATAATTG CTCAGGCTGA GTATGGCAAT
    GTAGATATTG GGGAAGAGGT GGCTAAGAAA GGATTTGCAG AAAAATGCAG ACTCACTTCC
    AGCACTAATA TCTGTGAGGA AAAAAAATCA GATCCTGGTC AGCTTGTCCT CAGGAACCTC
    AAAAGCCCCA TTCCTTTGTG GGGGCATAGA TCAAGCCAAT CAAACTTCAC CCGGCCAAAG
    GGGCGCTTAA GTGGGAGAAT GTGTCTTGAC TTGAAGAATG AAAATGATGC AGGAAATAAT
    GTGACATTTC CAAAGGAAAA TTTGGCTGTT GGTGACTTTA ATTTAGGGTC TAATGTCAGC
    CTGGCAAAAA TTAAGCAGGA CCAGAAACTG ATTGAAGAAA ATGAAAAACT TAAAACAGAG
    AAGGAGGTTC TTCTTGAAAG GTGCAAGGCA TTAGAATTGA AAGTCGAACA GACTGCCCAG
    GAGCTGCAGC AAGAGAAAGC AACTACCATG GATTTGACTA AACGTTTGGA AAGCACTGTG
    AAGACTTGTA TAGGTACCAG AATGAAAAAT CTGGCAGCTA AGGTTGAAAT ATTGAAAGAA
    ATTAGACGTG TCAGTATCAG TGTTCGTTTT GGAAATGACC TTTCAGATGC TATACAAGTG
    TTGGATGAAG ACTGCTTTAC TATCCCAGCT TCTTTGAATG AATTAGAGCT AATTTGGGCA
    GAATATAATC TGGCTCAGGA GAAGATTCAA ACTTGTGAGA ATGTGAATGA AGGGAATATT
    TTGATTGCTG AAAGAAATGA AGTACAACAG AAGCTGTACA TGGCAGTAGA AGTTTTTATT
    CTGGAAGTTG ATGAGTTATC TCTTAATAAA CGCTTAAAAA CGTTGCAGGA TTTGTCAGCC
    TCTTTAGAAG CAGTATATGG ACAGGCCAAA GAAGGAATTA ACTCTGAAGA AATACTGGAG
    AAATTTAATG ACTGGAGGTG TAATAAAAGA GAGGAGTTCA CCAGTGTTAG AAGTGAAACA
    GAGACTTGTA TACAACGTCT TATAGCATGG TTCCAGAATA CTTTAAAGGC CTTTGATCTG
    TCTGTGGACA AATCACTGAT TTCTGATGAC ACAATTGATA ATATTGATGA AATCCTGGAG
    AACACTGAGT CAGGTGTCTG CAAGGAGCTG GAGATATCTC TGATTGAACA AGGTGATGCA
    GACAAGGAGA TCATTTTAAA AACATGTAGT CAAGTACTAC AAAAGATCCG TTCAGAGGAA
    AGCCTTATTG CCACAGTACA ATCCAAGTAC AAGGACAGTG TTGAGTTTAA GAAACAGATT
    ATTGAATGTT TAAATAAGAG CCCCAGCGTG GATCACTTGC TTTCCATTAA AAAGACACTG
    AAAAGCTTAA AGGCTCGACT GAGATGGAAA TTGGTTGAAA AGAATAATTT GGAAGAATCT
    GATGACCATG ATGGATCTGA GATTGAAAAA ATAAAACAAG AAATAACTCA GTTACGGAAT
    AGTGTCTTTG AGGGAATTTA TCATGAGCAG GAGGAATATG CGAAGCTGAG TAGCTTAGTA
    CAAAAATGGT TCCCTGAGCT TCCTCTACTT CATCCTGAAA TAGGATTACT TAAGTACATG
    AACTCTGGTG GTCTTCTTAC AATGAGCTTG GAAAGAGATC TTCTTGATGC TGAGCCTATG
    AAGGAGCTTA GCAGCAAGCG TCCTTTGATA TGTTCTGAGG TTAATGGGCA GATAATTCTT
    TTAAAGGGCT ATTCTGTGGA TGTCGACACA GAAGCCAGGG TAATTCAGAG AGCAGCTGCC
    TATCACACAG CTTGGAGAGA AGCTAAAGGA GAATCTGGGT TGCTACCACT GATATTCTTA
    TTTTTGTGTA AGTCGGATCC TATGGCTTAT CTGATGGTCC CATATTATCC TAGGGCAAAT
    CTGAATGCTG TTCAGTCCAC CATGCCTTTA ACTTCAGAAG AAGTTTTAAA GGTCATGAAA
    GGTGTAGCCC AGGGCCTTCA TACATTGCAC AAGGCTGACA TAGTTCATGG ATCACTTCAT
    CAGAACAATG TTTTTGCTTT AAACCGTCAA CATGGAATTG TTGGAGATTT TGACTTCACC
    AAATCTGTGA GTCAGCGAAC CTCAGTGAAC ATGATGGTTG GTGACTTAAG TTTGCTATCA
    CCTGAACTGA AAATGGGAAA ACCTGCTTCT CCGAGTTCTG ACTTATATGC TTATGGCTGC
    CTTTTATTGT GGCTTTCAGT TCAGAATCAG GAATTTGAAA CTAATGAGGA TGGAATCCCA
    AAGATGGATC AGTTTCATTT GGATGATAAT GTCAAATCCC TCCTCTGTAG CTTGATCTGT
    TGTAGAAGTT CAATGACTGC CGAGCAGGTT TTGAATGCTG ACTGTTTCTT GCTACCAAAG
    GGAAAATCAA TGCCAAACTC AGAGAAAGAT ACTGAATACA TCCCCAAAAA AGAGGAAGAA
    GAATTAAAGG TGAAGAACTT GGATAAAGAT AAGGAAAAGA CAAGAAATGG TGAAGCCAGC
    TTTGATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015325215.1
    CDS121..3189
    Translation

    Target ORF information:

    RefSeq Version XM_015469729.1
    Organism Bos taurus(cattle)
    Definition Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015469729.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCCATGG ACCACCCATT TCTAAAGGCA TTTCAAGCAT CATCTAGCTC TTCTCCAGAA 
    ACGCTAACAG GAATTGTCCA AATGGAGGAA GATAAACATT GCAATAAAGT TGAAGATGTG
    GTTGGAAGTC ATGTGGAAGA TGCAGTAACA TTTTGGGCCC AGAGTATCAA TAGAAATAAG
    GATATTATGA AGATTGGTTG TTCACTGTCT GAAGTCTGTC CCCTTGCCAA TTCAGTGTTT
    GGGAATCTTG ATCCAAAGAA GATTTATGGT GGATTATTTT CTGAAGATAA ATGTTGGTAC
    AGATGCCAAG TACTGAAAGT CATCAACGAT GAAAAGTGTC TGGTGAGATA TATTGACTAT
    GGAAATACAG AAATTCTGAA TCGATCGGAT ATAGTAGAGA TTCCTTTGGA TCTGCAATTT
    TCTAGTGTTG CCAAAAAGTA TAGACTTTGG GGACTACAGA TTCCTTCTAG CCAAGAAGTT
    ACCCAGTTTG ATCAGGGCAG AACCTTTTTG GGGAGCTTGA TTTTTGAAAA GGAAATTAAA
    ATGAGAATTA AAGCTACCTA TCAAGATGGA ACAATAATTG CTCAGGCTGA GTATGGCAAT
    GTAGATATTG GGGAAGAGGT GGCTAAGAAA GGATTTGCAG AAAAATGCAG ACTCACTTCC
    AGCACTAATA TCTGTGAGGA AAAAAAATCA GATCCTGGTC AGCTTGTCCT CAGGAACCTC
    AAAAGCCCCA TTCCTTTGTG GGGGCATAGA TCAAGCCAAT CAAACTTCAC CCGGCCAAAG
    GGGCGCTTAA GTGGGAGAAT GTGTCTTGAC TTGAAGAATG AAAATGATGC AGGAAATAAT
    GTGACATTTC CAAAGGAAAA TTTGGCTGTT GGTGACTTTA ATTTAGGGTC TAATGTCAGC
    CTGGCAAAAA TTAAGCAGGA CCAGAAACTG ATTGAAGAAA ATGAAAAACT TAAAACAGAG
    AAGGAGGTTC TTCTTGAAAG GTGCAAGGCA TTAGAATTGA AAGTCGAACA GACTGCCCAG
    GAGCTGCAGC AAGAGAAAGC AACTACCATG GATTTGACTA AACGTTTGGA AAGCACTGTG
    AAGACTTGTA TAGGTACCAG AATGAAAAAT CTGGCAGCTA AGGTTGAAAT ATTGAAAGAA
    ATTAGACGTG TCAGTATCAG TGTTCGTTTT GGAAATGACC TTTCAGATGC TATACAAGTG
    TTGGATGAAG ACTGCTTTAC TATCCCAGCT TCTTTGAATG AATTAGAGCT AATTTGGGCA
    GAATATAATC TGGCTCAGGA GAAGATTCAA ACTTGTGAGA ATGTGAATGA AGGGAATATT
    TTGATTGCTG AAAGAAATGA AGTACAACAG AAGCTGTACA TGGCAGTAGA AGTTTTTATT
    CTGGAAGTTG ATGAGTTATC TCTTAATAAA CGCTTAAAAA CGTTGCAGGA TTTGTCAGCC
    TCTTTAGAAG CAGTATATGG ACAGGCCAAA GAAGGAATTA ACTCTGAAGA AATACTGGAG
    AAATTTAATG ACTGGAGGTG TAATAAAAGA GAGGAGTTCA CCAGTGTTAG AAGTGAAACA
    GAGACTTGTA TACAACGTCT TATAGCATGG TTCCAGAATA CTTTAAAGGC CTTTGATCTG
    TCTGTGGACA AATCACTGAT TTCTGATGAC ACAATTGATA ATATTGATGA AATCCTGGAG
    AACACTGAGT CAGGTGTCTG CAAGGAGCTG GAGATATCTC TGATTGAACA AGGTGATGCA
    GACAAGGAGA TCATTTTAAA AACATGTAGT CAAGTACTAC AAAAGATCCG TTCAGAGGAA
    AGCCTTATTG CCACAGTACA ATCCAAGTAC AAGGACAGTG TTGAGTTTAA GAAACAGATT
    ATTGAATGTT TAAATAAGAG CCCCAGCGTG GATCACTTGC TTTCCATTAA AAAGACACTG
    AAAAGCTTAA AGGCTCGACT GAGATGGAAA TTGGTTGAAA AGAATAATTT GGAAGAATCT
    GATGACCATG ATGGATCTGA GATTGAAAAA ATAAAACAAG AAATAACTCA GTTACGGAAT
    AGTGTCTTTG AGGGAATTTA TCATGAGCAG GAGGAATATG CGAAGCTGAG TAGCTTAGTA
    CAAAAATGGT TCCCTGAGCT TCCTCTACTT CATCCTGAAA TAGGATTACT TAAGTACATG
    AACTCTGGTG GTCTTCTTAC AATGAGCTTG GAAAGAGATC TTCTTGATGC TGAGCCTATG
    AAGGAGCTTA GCAGCAAGCG TCCTTTGATA TGTTCTGAGG TTAATGGGCA GATAATTCTT
    TTAAAGGGCT ATTCTGTGGA TGTCGACACA GAAGCCAGGG TAATTCAGAG AGCAGCTGCC
    TATCACACAG CTTGGAGAGA AGCTAAAGGA GAATCTGGGT TGCTACCACT GATATTCTTA
    TTTTTGTGTA AGTCGGATCC TATGGCTTAT CTGATGGTCC CATATTATCC TAGGGCAAAT
    CTGAATGCTG TTCAGTCCAC CATGCCTTTA ACTTCAGAAG AAGTTTTAAA GGTCATGAAA
    GGTGTAGCCC AGGGCCTTCA TACATTGCAC AAGGCTGACA TAGTTCATGG ATCACTTCAT
    CAGAACAATG TTTTTGCTTT AAACCGTCAA CATGGAATTG TTGGAGATTT TGACTTCACC
    AAATCTGTGA GTCAGCGAAC CTCAGTGAAC ATGATGGTTG GTGACTTAAG TTTGCTATCA
    CCTGAACTGA AAATGGGAAA ACCTGCTTCT CCGAGTTCTG ACTTATATGC TTATGGCTGC
    CTTTTATTGT GGCTTTCAGT TCAGAATCAG GAATTTGAAA CTAATGAGGA TGGAATCCCA
    AAGATGGATC AGTTTCATTT GGATGATAAT GTCAAATCCC TCCTCTGTAG CTTGATCTGT
    TGTAGAAGTT CAATGACTGC CGAGCAGGTT TTGAATGCTG ACTGTTTCTT GCTACCAAAG
    GGAAAATCAA TGCCAAACTC AGAGAAAGAT ACTGAATACA TCCCCAAAAA AGAGGAAGAA
    GAATTAAAGG TGAAGAACTT GGATAAAGAT AAGGAAAAGA CAAGAAATGG TGAAGCCAGC
    TTTGATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90627
    Clone ID Related Accession (Same CDS sequence) XM_015469725.1 , XM_015469729.1 , XM_024990536.1 , XM_015469725.2
    Accession Version XM_024990536.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3069bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein FAM221A isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCCATGG ACCACCCATT TCTAAAGGCA TTTCAAGCAT CATCTAGCTC TTCTCCAGAA 
    ACGCTAACAG GAATTGTCCA AATGGAGGAA GATAAACATT GCAATAAAGT TGAAGATGTG
    GTTGGAAGTC ATGTGGAAGA TGCAGTAACA TTTTGGGCCC AGAGTATCAA TAGAAATAAG
    GATATTATGA AGATTGGTTG TTCACTGTCT GAAGTCTGTC CCCTTGCCAA TTCAGTGTTT
    GGGAATCTTG ATCCAAAGAA GATTTATGGT GGATTATTTT CTGAAGATAA ATGTTGGTAC
    AGATGCCAAG TACTGAAAGT CATCAACGAT GAAAAGTGTC TGGTGAGATA TATTGACTAT
    GGAAATACAG AAATTCTGAA TCGATCGGAT ATAGTAGAGA TTCCTTTGGA TCTGCAATTT
    TCTAGTGTTG CCAAAAAGTA TAGACTTTGG GGACTACAGA TTCCTTCTAG CCAAGAAGTT
    ACCCAGTTTG ATCAGGGCAG AACCTTTTTG GGGAGCTTGA TTTTTGAAAA GGAAATTAAA
    ATGAGAATTA AAGCTACCTA TCAAGATGGA ACAATAATTG CTCAGGCTGA GTATGGCAAT
    GTAGATATTG GGGAAGAGGT GGCTAAGAAA GGATTTGCAG AAAAATGCAG ACTCACTTCC
    AGCACTAATA TCTGTGAGGA AAAAAAATCA GATCCTGGTC AGCTTGTCCT CAGGAACCTC
    AAAAGCCCCA TTCCTTTGTG GGGGCATAGA TCAAGCCAAT CAAACTTCAC CCGGCCAAAG
    GGGCGCTTAA GTGGGAGAAT GTGTCTTGAC TTGAAGAATG AAAATGATGC AGGAAATAAT
    GTGACATTTC CAAAGGAAAA TTTGGCTGTT GGTGACTTTA ATTTAGGGTC TAATGTCAGC
    CTGGCAAAAA TTAAGCAGGA CCAGAAACTG ATTGAAGAAA ATGAAAAACT TAAAACAGAG
    AAGGAGGTTC TTCTTGAAAG GTGCAAGGCA TTAGAATTGA AAGTCGAACA GACTGCCCAG
    GAGCTGCAGC AAGAGAAAGC AACTACCATG GATTTGACTA AACGTTTGGA AAGCACTGTG
    AAGACTTGTA TAGGTACCAG AATGAAAAAT CTGGCAGCTA AGGTTGAAAT ATTGAAAGAA
    ATTAGACGTG TCAGTATCAG TGTTCGTTTT GGAAATGACC TTTCAGATGC TATACAAGTG
    TTGGATGAAG ACTGCTTTAC TATCCCAGCT TCTTTGAATG AATTAGAGCT AATTTGGGCA
    GAATATAATC TGGCTCAGGA GAAGATTCAA ACTTGTGAGA ATGTGAATGA AGGGAATATT
    TTGATTGCTG AAAGAAATGA AGTACAACAG AAGCTGTACA TGGCAGTAGA AGTTTTTATT
    CTGGAAGTTG ATGAGTTATC TCTTAATAAA CGCTTAAAAA CGTTGCAGGA TTTGTCAGCC
    TCTTTAGAAG CAGTATATGG ACAGGCCAAA GAAGGAATTA ACTCTGAAGA AATACTGGAG
    AAATTTAATG ACTGGAGGTG TAATAAAAGA GAGGAGTTCA CCAGTGTTAG AAGTGAAACA
    GAGACTTGTA TACAACGTCT TATAGCATGG TTCCAGAATA CTTTAAAGGC CTTTGATCTG
    TCTGTGGACA AATCACTGAT TTCTGATGAC ACAATTGATA ATATTGATGA AATCCTGGAG
    AACACTGAGT CAGGTGTCTG CAAGGAGCTG GAGATATCTC TGATTGAACA AGGTGATGCA
    GACAAGGAGA TCATTTTAAA AACATGTAGT CAAGTACTAC AAAAGATCCG TTCAGAGGAA
    AGCCTTATTG CCACAGTACA ATCCAAGTAC AAGGACAGTG TTGAGTTTAA GAAACAGATT
    ATTGAATGTT TAAATAAGAG CCCCAGCGTG GATCACTTGC TTTCCATTAA AAAGACACTG
    AAAAGCTTAA AGGCTCGACT GAGATGGAAA TTGGTTGAAA AGAATAATTT GGAAGAATCT
    GATGACCATG ATGGATCTGA GATTGAAAAA ATAAAACAAG AAATAACTCA GTTACGGAAT
    AGTGTCTTTG AGGGAATTTA TCATGAGCAG GAGGAATATG CGAAGCTGAG TAGCTTAGTA
    CAAAAATGGT TCCCTGAGCT TCCTCTACTT CATCCTGAAA TAGGATTACT TAAGTACATG
    AACTCTGGTG GTCTTCTTAC AATGAGCTTG GAAAGAGATC TTCTTGATGC TGAGCCTATG
    AAGGAGCTTA GCAGCAAGCG TCCTTTGATA TGTTCTGAGG TTAATGGGCA GATAATTCTT
    TTAAAGGGCT ATTCTGTGGA TGTCGACACA GAAGCCAGGG TAATTCAGAG AGCAGCTGCC
    TATCACACAG CTTGGAGAGA AGCTAAAGGA GAATCTGGGT TGCTACCACT GATATTCTTA
    TTTTTGTGTA AGTCGGATCC TATGGCTTAT CTGATGGTCC CATATTATCC TAGGGCAAAT
    CTGAATGCTG TTCAGTCCAC CATGCCTTTA ACTTCAGAAG AAGTTTTAAA GGTCATGAAA
    GGTGTAGCCC AGGGCCTTCA TACATTGCAC AAGGCTGACA TAGTTCATGG ATCACTTCAT
    CAGAACAATG TTTTTGCTTT AAACCGTCAA CATGGAATTG TTGGAGATTT TGACTTCACC
    AAATCTGTGA GTCAGCGAAC CTCAGTGAAC ATGATGGTTG GTGACTTAAG TTTGCTATCA
    CCTGAACTGA AAATGGGAAA ACCTGCTTCT CCGAGTTCTG ACTTATATGC TTATGGCTGC
    CTTTTATTGT GGCTTTCAGT TCAGAATCAG GAATTTGAAA CTAATGAGGA TGGAATCCCA
    AAGATGGATC AGTTTCATTT GGATGATAAT GTCAAATCCC TCCTCTGTAG CTTGATCTGT
    TGTAGAAGTT CAATGACTGC CGAGCAGGTT TTGAATGCTG ACTGTTTCTT GCTACCAAAG
    GGAAAATCAA TGCCAAACTC AGAGAAAGAT ACTGAATACA TCCCCAAAAA AGAGGAAGAA
    GAATTAAAGG TGAAGAACTT GGATAAAGAT AAGGAAAAGA CAAGAAATGG TGAAGCCAGC
    TTTGATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024846304.1
    CDS524..3592
    Translation

    Target ORF information:

    RefSeq Version XM_024990536.1
    Organism Bos taurus(cattle)
    Definition Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024990536.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCCATGG ACCACCCATT TCTAAAGGCA TTTCAAGCAT CATCTAGCTC TTCTCCAGAA 
    ACGCTAACAG GAATTGTCCA AATGGAGGAA GATAAACATT GCAATAAAGT TGAAGATGTG
    GTTGGAAGTC ATGTGGAAGA TGCAGTAACA TTTTGGGCCC AGAGTATCAA TAGAAATAAG
    GATATTATGA AGATTGGTTG TTCACTGTCT GAAGTCTGTC CCCTTGCCAA TTCAGTGTTT
    GGGAATCTTG ATCCAAAGAA GATTTATGGT GGATTATTTT CTGAAGATAA ATGTTGGTAC
    AGATGCCAAG TACTGAAAGT CATCAACGAT GAAAAGTGTC TGGTGAGATA TATTGACTAT
    GGAAATACAG AAATTCTGAA TCGATCGGAT ATAGTAGAGA TTCCTTTGGA TCTGCAATTT
    TCTAGTGTTG CCAAAAAGTA TAGACTTTGG GGACTACAGA TTCCTTCTAG CCAAGAAGTT
    ACCCAGTTTG ATCAGGGCAG AACCTTTTTG GGGAGCTTGA TTTTTGAAAA GGAAATTAAA
    ATGAGAATTA AAGCTACCTA TCAAGATGGA ACAATAATTG CTCAGGCTGA GTATGGCAAT
    GTAGATATTG GGGAAGAGGT GGCTAAGAAA GGATTTGCAG AAAAATGCAG ACTCACTTCC
    AGCACTAATA TCTGTGAGGA AAAAAAATCA GATCCTGGTC AGCTTGTCCT CAGGAACCTC
    AAAAGCCCCA TTCCTTTGTG GGGGCATAGA TCAAGCCAAT CAAACTTCAC CCGGCCAAAG
    GGGCGCTTAA GTGGGAGAAT GTGTCTTGAC TTGAAGAATG AAAATGATGC AGGAAATAAT
    GTGACATTTC CAAAGGAAAA TTTGGCTGTT GGTGACTTTA ATTTAGGGTC TAATGTCAGC
    CTGGCAAAAA TTAAGCAGGA CCAGAAACTG ATTGAAGAAA ATGAAAAACT TAAAACAGAG
    AAGGAGGTTC TTCTTGAAAG GTGCAAGGCA TTAGAATTGA AAGTCGAACA GACTGCCCAG
    GAGCTGCAGC AAGAGAAAGC AACTACCATG GATTTGACTA AACGTTTGGA AAGCACTGTG
    AAGACTTGTA TAGGTACCAG AATGAAAAAT CTGGCAGCTA AGGTTGAAAT ATTGAAAGAA
    ATTAGACGTG TCAGTATCAG TGTTCGTTTT GGAAATGACC TTTCAGATGC TATACAAGTG
    TTGGATGAAG ACTGCTTTAC TATCCCAGCT TCTTTGAATG AATTAGAGCT AATTTGGGCA
    GAATATAATC TGGCTCAGGA GAAGATTCAA ACTTGTGAGA ATGTGAATGA AGGGAATATT
    TTGATTGCTG AAAGAAATGA AGTACAACAG AAGCTGTACA TGGCAGTAGA AGTTTTTATT
    CTGGAAGTTG ATGAGTTATC TCTTAATAAA CGCTTAAAAA CGTTGCAGGA TTTGTCAGCC
    TCTTTAGAAG CAGTATATGG ACAGGCCAAA GAAGGAATTA ACTCTGAAGA AATACTGGAG
    AAATTTAATG ACTGGAGGTG TAATAAAAGA GAGGAGTTCA CCAGTGTTAG AAGTGAAACA
    GAGACTTGTA TACAACGTCT TATAGCATGG TTCCAGAATA CTTTAAAGGC CTTTGATCTG
    TCTGTGGACA AATCACTGAT TTCTGATGAC ACAATTGATA ATATTGATGA AATCCTGGAG
    AACACTGAGT CAGGTGTCTG CAAGGAGCTG GAGATATCTC TGATTGAACA AGGTGATGCA
    GACAAGGAGA TCATTTTAAA AACATGTAGT CAAGTACTAC AAAAGATCCG TTCAGAGGAA
    AGCCTTATTG CCACAGTACA ATCCAAGTAC AAGGACAGTG TTGAGTTTAA GAAACAGATT
    ATTGAATGTT TAAATAAGAG CCCCAGCGTG GATCACTTGC TTTCCATTAA AAAGACACTG
    AAAAGCTTAA AGGCTCGACT GAGATGGAAA TTGGTTGAAA AGAATAATTT GGAAGAATCT
    GATGACCATG ATGGATCTGA GATTGAAAAA ATAAAACAAG AAATAACTCA GTTACGGAAT
    AGTGTCTTTG AGGGAATTTA TCATGAGCAG GAGGAATATG CGAAGCTGAG TAGCTTAGTA
    CAAAAATGGT TCCCTGAGCT TCCTCTACTT CATCCTGAAA TAGGATTACT TAAGTACATG
    AACTCTGGTG GTCTTCTTAC AATGAGCTTG GAAAGAGATC TTCTTGATGC TGAGCCTATG
    AAGGAGCTTA GCAGCAAGCG TCCTTTGATA TGTTCTGAGG TTAATGGGCA GATAATTCTT
    TTAAAGGGCT ATTCTGTGGA TGTCGACACA GAAGCCAGGG TAATTCAGAG AGCAGCTGCC
    TATCACACAG CTTGGAGAGA AGCTAAAGGA GAATCTGGGT TGCTACCACT GATATTCTTA
    TTTTTGTGTA AGTCGGATCC TATGGCTTAT CTGATGGTCC CATATTATCC TAGGGCAAAT
    CTGAATGCTG TTCAGTCCAC CATGCCTTTA ACTTCAGAAG AAGTTTTAAA GGTCATGAAA
    GGTGTAGCCC AGGGCCTTCA TACATTGCAC AAGGCTGACA TAGTTCATGG ATCACTTCAT
    CAGAACAATG TTTTTGCTTT AAACCGTCAA CATGGAATTG TTGGAGATTT TGACTTCACC
    AAATCTGTGA GTCAGCGAAC CTCAGTGAAC ATGATGGTTG GTGACTTAAG TTTGCTATCA
    CCTGAACTGA AAATGGGAAA ACCTGCTTCT CCGAGTTCTG ACTTATATGC TTATGGCTGC
    CTTTTATTGT GGCTTTCAGT TCAGAATCAG GAATTTGAAA CTAATGAGGA TGGAATCCCA
    AAGATGGATC AGTTTCATTT GGATGATAAT GTCAAATCCC TCCTCTGTAG CTTGATCTGT
    TGTAGAAGTT CAATGACTGC CGAGCAGGTT TTGAATGCTG ACTGTTTCTT GCTACCAAAG
    GGAAAATCAA TGCCAAACTC AGAGAAAGAT ACTGAATACA TCCCCAAAAA AGAGGAAGAA
    GAATTAAAGG TGAAGAACTT GGATAAAGAT AAGGAAAAGA CAAGAAATGG TGAAGCCAGC
    TTTGATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90627
    Clone ID Related Accession (Same CDS sequence) XM_015469725.1 , XM_015469729.1 , XM_024990536.1 , XM_015469725.2
    Accession Version XM_015469725.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3069bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein FAM221A isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015469725.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCCATGG ACCACCCATT TCTAAAGGCA TTTCAAGCAT CATCTAGCTC TTCTCCAGAA 
    ACGCTAACAG GAATTGTCCA AATGGAGGAA GATAAACATT GCAATAAAGT TGAAGATGTG
    GTTGGAAGTC ATGTGGAAGA TGCAGTAACA TTTTGGGCCC AGAGTATCAA TAGAAATAAG
    GATATTATGA AGATTGGTTG TTCACTGTCT GAAGTCTGTC CCCTTGCCAA TTCAGTGTTT
    GGGAATCTTG ATCCAAAGAA GATTTATGGT GGATTATTTT CTGAAGATAA ATGTTGGTAC
    AGATGCCAAG TACTGAAAGT CATCAACGAT GAAAAGTGTC TGGTGAGATA TATTGACTAT
    GGAAATACAG AAATTCTGAA TCGATCGGAT ATAGTAGAGA TTCCTTTGGA TCTGCAATTT
    TCTAGTGTTG CCAAAAAGTA TAGACTTTGG GGACTACAGA TTCCTTCTAG CCAAGAAGTT
    ACCCAGTTTG ATCAGGGCAG AACCTTTTTG GGGAGCTTGA TTTTTGAAAA GGAAATTAAA
    ATGAGAATTA AAGCTACCTA TCAAGATGGA ACAATAATTG CTCAGGCTGA GTATGGCAAT
    GTAGATATTG GGGAAGAGGT GGCTAAGAAA GGATTTGCAG AAAAATGCAG ACTCACTTCC
    AGCACTAATA TCTGTGAGGA AAAAAAATCA GATCCTGGTC AGCTTGTCCT CAGGAACCTC
    AAAAGCCCCA TTCCTTTGTG GGGGCATAGA TCAAGCCAAT CAAACTTCAC CCGGCCAAAG
    GGGCGCTTAA GTGGGAGAAT GTGTCTTGAC TTGAAGAATG AAAATGATGC AGGAAATAAT
    GTGACATTTC CAAAGGAAAA TTTGGCTGTT GGTGACTTTA ATTTAGGGTC TAATGTCAGC
    CTGGCAAAAA TTAAGCAGGA CCAGAAACTG ATTGAAGAAA ATGAAAAACT TAAAACAGAG
    AAGGAGGTTC TTCTTGAAAG GTGCAAGGCA TTAGAATTGA AAGTCGAACA GACTGCCCAG
    GAGCTGCAGC AAGAGAAAGC AACTACCATG GATTTGACTA AACGTTTGGA AAGCACTGTG
    AAGACTTGTA TAGGTACCAG AATGAAAAAT CTGGCAGCTA AGGTTGAAAT ATTGAAAGAA
    ATTAGACGTG TCAGTATCAG TGTTCGTTTT GGAAATGACC TTTCAGATGC TATACAAGTG
    TTGGATGAAG ACTGCTTTAC TATCCCAGCT TCTTTGAATG AATTAGAGCT AATTTGGGCA
    GAATATAATC TGGCTCAGGA GAAGATTCAA ACTTGTGAGA ATGTGAATGA AGGGAATATT
    TTGATTGCTG AAAGAAATGA AGTACAACAG AAGCTGTACA TGGCAGTAGA AGTTTTTATT
    CTGGAAGTTG ATGAGTTATC TCTTAATAAA CGCTTAAAAA CGTTGCAGGA TTTGTCAGCC
    TCTTTAGAAG CAGTATATGG ACAGGCCAAA GAAGGAATTA ACTCTGAAGA AATACTGGAG
    AAATTTAATG ACTGGAGGTG TAATAAAAGA GAGGAGTTCA CCAGTGTTAG AAGTGAAACA
    GAGACTTGTA TACAACGTCT TATAGCATGG TTCCAGAATA CTTTAAAGGC CTTTGATCTG
    TCTGTGGACA AATCACTGAT TTCTGATGAC ACAATTGATA ATATTGATGA AATCCTGGAG
    AACACTGAGT CAGGTGTCTG CAAGGAGCTG GAGATATCTC TGATTGAACA AGGTGATGCA
    GACAAGGAGA TCATTTTAAA AACATGTAGT CAAGTACTAC AAAAGATCCG TTCAGAGGAA
    AGCCTTATTG CCACAGTACA ATCCAAGTAC AAGGACAGTG TTGAGTTTAA GAAACAGATT
    ATTGAATGTT TAAATAAGAG CCCCAGCGTG GATCACTTGC TTTCCATTAA AAAGACACTG
    AAAAGCTTAA AGGCTCGACT GAGATGGAAA TTGGTTGAAA AGAATAATTT GGAAGAATCT
    GATGACCATG ATGGATCTGA GATTGAAAAA ATAAAACAAG AAATAACTCA GTTACGGAAT
    AGTGTCTTTG AGGGAATTTA TCATGAGCAG GAGGAATATG CGAAGCTGAG TAGCTTAGTA
    CAAAAATGGT TCCCTGAGCT TCCTCTACTT CATCCTGAAA TAGGATTACT TAAGTACATG
    AACTCTGGTG GTCTTCTTAC AATGAGCTTG GAAAGAGATC TTCTTGATGC TGAGCCTATG
    AAGGAGCTTA GCAGCAAGCG TCCTTTGATA TGTTCTGAGG TTAATGGGCA GATAATTCTT
    TTAAAGGGCT ATTCTGTGGA TGTCGACACA GAAGCCAGGG TAATTCAGAG AGCAGCTGCC
    TATCACACAG CTTGGAGAGA AGCTAAAGGA GAATCTGGGT TGCTACCACT GATATTCTTA
    TTTTTGTGTA AGTCGGATCC TATGGCTTAT CTGATGGTCC CATATTATCC TAGGGCAAAT
    CTGAATGCTG TTCAGTCCAC CATGCCTTTA ACTTCAGAAG AAGTTTTAAA GGTCATGAAA
    GGTGTAGCCC AGGGCCTTCA TACATTGCAC AAGGCTGACA TAGTTCATGG ATCACTTCAT
    CAGAACAATG TTTTTGCTTT AAACCGTCAA CATGGAATTG TTGGAGATTT TGACTTCACC
    AAATCTGTGA GTCAGCGAAC CTCAGTGAAC ATGATGGTTG GTGACTTAAG TTTGCTATCA
    CCTGAACTGA AAATGGGAAA ACCTGCTTCT CCGAGTTCTG ACTTATATGC TTATGGCTGC
    CTTTTATTGT GGCTTTCAGT TCAGAATCAG GAATTTGAAA CTAATGAGGA TGGAATCCCA
    AAGATGGATC AGTTTCATTT GGATGATAAT GTCAAATCCC TCCTCTGTAG CTTGATCTGT
    TGTAGAAGTT CAATGACTGC CGAGCAGGTT TTGAATGCTG ACTGTTTCTT GCTACCAAAG
    GGAAAATCAA TGCCAAACTC AGAGAAAGAT ACTGAATACA TCCCCAAAAA AGAGGAAGAA
    GAATTAAAGG TGAAGAACTT GGATAAAGAT AAGGAAAAGA CAAGAAATGG TGAAGCCAGC
    TTTGATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015325211.1
    CDS178..3246
    Translation

    Target ORF information:

    RefSeq Version XM_015469725.2
    Organism Bos taurus(cattle)
    Definition Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015469725.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCCATGG ACCACCCATT TCTAAAGGCA TTTCAAGCAT CATCTAGCTC TTCTCCAGAA 
    ACGCTAACAG GAATTGTCCA AATGGAGGAA GATAAACATT GCAATAAAGT TGAAGATGTG
    GTTGGAAGTC ATGTGGAAGA TGCAGTAACA TTTTGGGCCC AGAGTATCAA TAGAAATAAG
    GATATTATGA AGATTGGTTG TTCACTGTCT GAAGTCTGTC CCCTTGCCAA TTCAGTGTTT
    GGGAATCTTG ATCCAAAGAA GATTTATGGT GGATTATTTT CTGAAGATAA ATGTTGGTAC
    AGATGCCAAG TACTGAAAGT CATCAACGAT GAAAAGTGTC TGGTGAGATA TATTGACTAT
    GGAAATACAG AAATTCTGAA TCGATCGGAT ATAGTAGAGA TTCCTTTGGA TCTGCAATTT
    TCTAGTGTTG CCAAAAAGTA TAGACTTTGG GGACTACAGA TTCCTTCTAG CCAAGAAGTT
    ACCCAGTTTG ATCAGGGCAG AACCTTTTTG GGGAGCTTGA TTTTTGAAAA GGAAATTAAA
    ATGAGAATTA AAGCTACCTA TCAAGATGGA ACAATAATTG CTCAGGCTGA GTATGGCAAT
    GTAGATATTG GGGAAGAGGT GGCTAAGAAA GGATTTGCAG AAAAATGCAG ACTCACTTCC
    AGCACTAATA TCTGTGAGGA AAAAAAATCA GATCCTGGTC AGCTTGTCCT CAGGAACCTC
    AAAAGCCCCA TTCCTTTGTG GGGGCATAGA TCAAGCCAAT CAAACTTCAC CCGGCCAAAG
    GGGCGCTTAA GTGGGAGAAT GTGTCTTGAC TTGAAGAATG AAAATGATGC AGGAAATAAT
    GTGACATTTC CAAAGGAAAA TTTGGCTGTT GGTGACTTTA ATTTAGGGTC TAATGTCAGC
    CTGGCAAAAA TTAAGCAGGA CCAGAAACTG ATTGAAGAAA ATGAAAAACT TAAAACAGAG
    AAGGAGGTTC TTCTTGAAAG GTGCAAGGCA TTAGAATTGA AAGTCGAACA GACTGCCCAG
    GAGCTGCAGC AAGAGAAAGC AACTACCATG GATTTGACTA AACGTTTGGA AAGCACTGTG
    AAGACTTGTA TAGGTACCAG AATGAAAAAT CTGGCAGCTA AGGTTGAAAT ATTGAAAGAA
    ATTAGACGTG TCAGTATCAG TGTTCGTTTT GGAAATGACC TTTCAGATGC TATACAAGTG
    TTGGATGAAG ACTGCTTTAC TATCCCAGCT TCTTTGAATG AATTAGAGCT AATTTGGGCA
    GAATATAATC TGGCTCAGGA GAAGATTCAA ACTTGTGAGA ATGTGAATGA AGGGAATATT
    TTGATTGCTG AAAGAAATGA AGTACAACAG AAGCTGTACA TGGCAGTAGA AGTTTTTATT
    CTGGAAGTTG ATGAGTTATC TCTTAATAAA CGCTTAAAAA CGTTGCAGGA TTTGTCAGCC
    TCTTTAGAAG CAGTATATGG ACAGGCCAAA GAAGGAATTA ACTCTGAAGA AATACTGGAG
    AAATTTAATG ACTGGAGGTG TAATAAAAGA GAGGAGTTCA CCAGTGTTAG AAGTGAAACA
    GAGACTTGTA TACAACGTCT TATAGCATGG TTCCAGAATA CTTTAAAGGC CTTTGATCTG
    TCTGTGGACA AATCACTGAT TTCTGATGAC ACAATTGATA ATATTGATGA AATCCTGGAG
    AACACTGAGT CAGGTGTCTG CAAGGAGCTG GAGATATCTC TGATTGAACA AGGTGATGCA
    GACAAGGAGA TCATTTTAAA AACATGTAGT CAAGTACTAC AAAAGATCCG TTCAGAGGAA
    AGCCTTATTG CCACAGTACA ATCCAAGTAC AAGGACAGTG TTGAGTTTAA GAAACAGATT
    ATTGAATGTT TAAATAAGAG CCCCAGCGTG GATCACTTGC TTTCCATTAA AAAGACACTG
    AAAAGCTTAA AGGCTCGACT GAGATGGAAA TTGGTTGAAA AGAATAATTT GGAAGAATCT
    GATGACCATG ATGGATCTGA GATTGAAAAA ATAAAACAAG AAATAACTCA GTTACGGAAT
    AGTGTCTTTG AGGGAATTTA TCATGAGCAG GAGGAATATG CGAAGCTGAG TAGCTTAGTA
    CAAAAATGGT TCCCTGAGCT TCCTCTACTT CATCCTGAAA TAGGATTACT TAAGTACATG
    AACTCTGGTG GTCTTCTTAC AATGAGCTTG GAAAGAGATC TTCTTGATGC TGAGCCTATG
    AAGGAGCTTA GCAGCAAGCG TCCTTTGATA TGTTCTGAGG TTAATGGGCA GATAATTCTT
    TTAAAGGGCT ATTCTGTGGA TGTCGACACA GAAGCCAGGG TAATTCAGAG AGCAGCTGCC
    TATCACACAG CTTGGAGAGA AGCTAAAGGA GAATCTGGGT TGCTACCACT GATATTCTTA
    TTTTTGTGTA AGTCGGATCC TATGGCTTAT CTGATGGTCC CATATTATCC TAGGGCAAAT
    CTGAATGCTG TTCAGTCCAC CATGCCTTTA ACTTCAGAAG AAGTTTTAAA GGTCATGAAA
    GGTGTAGCCC AGGGCCTTCA TACATTGCAC AAGGCTGACA TAGTTCATGG ATCACTTCAT
    CAGAACAATG TTTTTGCTTT AAACCGTCAA CATGGAATTG TTGGAGATTT TGACTTCACC
    AAATCTGTGA GTCAGCGAAC CTCAGTGAAC ATGATGGTTG GTGACTTAAG TTTGCTATCA
    CCTGAACTGA AAATGGGAAA ACCTGCTTCT CCGAGTTCTG ACTTATATGC TTATGGCTGC
    CTTTTATTGT GGCTTTCAGT TCAGAATCAG GAATTTGAAA CTAATGAGGA TGGAATCCCA
    AAGATGGATC AGTTTCATTT GGATGATAAT GTCAAATCCC TCCTCTGTAG CTTGATCTGT
    TGTAGAAGTT CAATGACTGC CGAGCAGGTT TTGAATGCTG ACTGTTTCTT GCTACCAAAG
    GGAAAATCAA TGCCAAACTC AGAGAAAGAT ACTGAATACA TCCCCAAAAA AGAGGAAGAA
    GAATTAAAGG TGAAGAACTT GGATAAAGAT AAGGAAAAGA CAAGAAATGG TGAAGCCAGC
    TTTGATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18655
    Clone ID Related Accession (Same CDS sequence) XM_005205559.3 , XM_015463068.1 , XM_005205559.4
    Accession Version XM_005205559.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3069bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein FAM221A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103900.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005205559.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGTTGGCAC AGCAACGAGC GCGCAAGCGC CGTTTGGAGA CCCAGTGGCC AGAACACGTG 
    AGTTTTGCAG GAATTGTCCA AATGGAGGAA GATAAACATT GCAATAAAGT TGAAGATGTG
    GTTGGAAGTC ATGTGGAAGA TGCAGTAACA TTTTGGGCCC AGAGTATCAA TAGAAATAAG
    GATATTATGA AGATTGGTTG TTCACTGTCT GAAGTCTGTC CCCTTGCCAA TTCAGTGTTT
    GGGAATCTTG ATCCAAAGAA GATTTATGGT GGATTATTTT CTGAAGATAA ATGTTGGTAC
    AGATGCCAAG TACTGAAAGT CATCAACGAT GAAAAGTGTC TGGTGAGATA TATTGACTAT
    GGAAATACAG AAATTCTGAA TCGATCGGAT ATAGTAGAGA TTCCTTTGGA TCTGCAATTT
    TCTAGTGTTG CCAAAAAGTA TAGACTTTGG GGACTACAGA TTCCTTCTAG CCAAGAAGTT
    ACCCAGTTTG ATCAGGGCAG AACCTTTTTG GGGAGCTTGA TTTTTGAAAA GGAAATTAAA
    ATGAGAATTA AAGCTACCTA TCAAGATGGA ACAATAATTG CTCAGGCTGA GTATGGCAAT
    GTAGATATTG GGGAAGAGGT GGCTAAGAAA GGATTTGCAG AAAAATGCAG ACTCACTTCC
    AGCACTAATA TCTGTGAGGA AAAAAAATCA GATCCTGGTC AGCTTGTCCT CAGGAACCTC
    AAAAGCCCCA TTCCTTTGTG GGGGCATAGA TCAAGCCAAT CAAACTTCAC CCGGCCAAAG
    GGGCGCTTAA GTGGGAGAAT GTGTCTTGAC TTGAAGAATG AAAATGATGC AGGAAATAAT
    GTGACATTTC CAAAGGAAAA TTTGGCTGTT GGTGACTTTA ATTTAGGGTC TAATGTCAGC
    CTGGCAAAAA TTAAGCAGGA CCAGAAACTG ATTGAAGAAA ATGAAAAACT TAAAACAGAG
    AAGGAGGTTC TTCTTGAAAG GTGCAAGGCA TTAGAATTGA AAGTCGAACA GACTGCCCAG
    GAGCTGCAGC AAGAGAAAGC AACTACCATG GATTTGACTA AACGTTTGGA AAGCACTGTG
    AAGACTTGTA TAGGTACCAG AATGAAAAAT CTGGCAGCTA AGGTTGAAAT ATTGAAAGAA
    ATTAGACGTG TCAGTATCAG TGTTCGTTTT GGAAATGACC TTTCAGATGC TATACAAGTG
    TTGGATGAAG ACTGCTTTAC TATCCCAGCT TCTTTGAATG AATTAGAGCT AATTTGGGCA
    GAATATAATC TGGCTCAGGA GAAGATTCAA ACTTGTGAGA ATGTGAATGA AGGGAATATT
    TTGATTGCTG AAAGAAATGA AGTACAACAG AAGCTGTACA TGGCAGTAGA AGTTTTTATT
    CTGGAAGTTG ATGAGTTATC TCTTAATAAA CGCTTAAAAA CGTTGCAGGA TTTGTCAGCC
    TCTTTAGAAG CAGTATATGG ACAGGCCAAA GAAGGAATTA ACTCTGAAGA AATACTGGAG
    AAATTTAATG ACTGGAGGTG TAATAAAAGA GAGGAGTTCA CCAGTGTTAG AAGTGAAACA
    GAGACTTGTA TACAACGTCT TATAGCATGG TTCCAGAATA CTTTAAAGGC CTTTGATCTG
    TCTGTGGACA AATCACTGAT TTCTGATGAC ACAATTGATA ATATTGATGA AATCCTGGAG
    AACACTGAGT CAGGTGTCTG CAAGGAGCTG GAGATATCTC TGATTGAACA AGGTGATGCA
    GACAAGGAGA TCATTTTAAA AACATGTAGT CAAGTACTAC AAAAGATCCG TTCAGAGGAA
    AGCCTTATTG CCACAGTACA ATCCAAGTAC AAGGACAGTG TTGAGTTTAA GAAACAGATT
    ATTGAATGTT TAAATAAGAG CCCCAGCGTG GATCACTTGC TTTCCATTAA AAAGACACTG
    AAAAGCTTAA AGGCTCGACT GAGATGGAAA TTGGTTGAAA AGAATAATTT GGAAGAATCT
    GATGACCATG ATGGATCTGA GATTGAAAAA ATAAAACAAG AAATAACTCA GTTACGGAAT
    AGTGTCTTTG AGGGAATTTA TCATGAGCAG GAGGAATATG CGAAGCTGAG TAGCTTAGTA
    CAAAAATGGT TCCCTGAGCT TCCTCTACTT CATCCTGAAA TAGGATTACT TAAGTACATG
    AACTCTGGTG GTCTTCTTAC AATGAGCTTG GAAAGAGATC TTCTTGATGC TGAGCCTATG
    AAGGAGCTTA GCAGCAAGCG TCCTTTGATA TGTTCTGAGG TTAATGGGCA GATAATTCTT
    TTAAAGGGCT ATTCTGTGGA TGTCGACACA GAAGCCAGGG TAATTCAGAG AGCAGCTGCC
    TATCACACAG CTTGGAGAGA AGCTAAAGGA GAATCTGGGT TGCTACCACT GATATTCTTA
    TTTTTGTGTA AGTCGGATCC TATGGCTTAT CTGATGGTCC CATATTATCC TAGGGCAAAT
    CTGAATGCTG TTCAGTCCAC CATGCCTTTA ACTTCAGAAG AAGTTTTAAA GGTCATGAAA
    GGTGTAGCCC AGGGCCTTCA TACATTGCAC AAGGCTGACA TAGTTCATGG ATCACTTCAT
    CAGAACAATG TTTTTGCTTT AAACCGTCAA CATGGAATTG TTGGAGATTT TGACTTCACC
    AAATCTGTGA GTCAGCGAAC CTCAGTGAAC ATGATGGTTG GTGACTTAAG TTTGCTATCA
    CCTGAACTGA AAATGGGAAA ACCTGCTTCT CCGAGTTCTG ACTTATATGC TTATGGCTGC
    CTTTTATTGT GGCTTTCAGT TCAGAATCAG GAATTTGAAA CTAATGAGGA TGGAATCCCA
    AAGATGGATC AGTTTCATTT GGATGATAAT GTCAAATCCC TCCTCTGTAG CTTGATCTGT
    TGTAGAAGTT CAATGACTGC CGAGCAGGTT TTGAATGCTG ACTGTTTCTT GCTACCAAAG
    GGAAAATCAA TGCCAAACTC AGAGAAAGAT ACTGAATACA TCCCCAAAAA AGAGGAAGAA
    GAATTAAAGG TGAAGAACTT GGATAAAGAT AAGGAAAAGA CAAGAAATGG TGAAGCCAGC
    TTTGATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005205616.1
    CDS398..3466
    Translation

    Target ORF information:

    RefSeq Version XM_005205559.3
    Organism Bos taurus(cattle)
    Definition Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005205559.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGTTGGCAC AGCAACGAGC GCGCAAGCGC CGTTTGGAGA CCCAGTGGCC AGAACACGTG 
    AGTTTTGCAG GAATTGTCCA AATGGAGGAA GATAAACATT GCAATAAAGT TGAAGATGTG
    GTTGGAAGTC ATGTGGAAGA TGCAGTAACA TTTTGGGCCC AGAGTATCAA TAGAAATAAG
    GATATTATGA AGATTGGTTG TTCACTGTCT GAAGTCTGTC CCCTTGCCAA TTCAGTGTTT
    GGGAATCTTG ATCCAAAGAA GATTTATGGT GGATTATTTT CTGAAGATAA ATGTTGGTAC
    AGATGCCAAG TACTGAAAGT CATCAACGAT GAAAAGTGTC TGGTGAGATA TATTGACTAT
    GGAAATACAG AAATTCTGAA TCGATCGGAT ATAGTAGAGA TTCCTTTGGA TCTGCAATTT
    TCTAGTGTTG CCAAAAAGTA TAGACTTTGG GGACTACAGA TTCCTTCTAG CCAAGAAGTT
    ACCCAGTTTG ATCAGGGCAG AACCTTTTTG GGGAGCTTGA TTTTTGAAAA GGAAATTAAA
    ATGAGAATTA AAGCTACCTA TCAAGATGGA ACAATAATTG CTCAGGCTGA GTATGGCAAT
    GTAGATATTG GGGAAGAGGT GGCTAAGAAA GGATTTGCAG AAAAATGCAG ACTCACTTCC
    AGCACTAATA TCTGTGAGGA AAAAAAATCA GATCCTGGTC AGCTTGTCCT CAGGAACCTC
    AAAAGCCCCA TTCCTTTGTG GGGGCATAGA TCAAGCCAAT CAAACTTCAC CCGGCCAAAG
    GGGCGCTTAA GTGGGAGAAT GTGTCTTGAC TTGAAGAATG AAAATGATGC AGGAAATAAT
    GTGACATTTC CAAAGGAAAA TTTGGCTGTT GGTGACTTTA ATTTAGGGTC TAATGTCAGC
    CTGGCAAAAA TTAAGCAGGA CCAGAAACTG ATTGAAGAAA ATGAAAAACT TAAAACAGAG
    AAGGAGGTTC TTCTTGAAAG GTGCAAGGCA TTAGAATTGA AAGTCGAACA GACTGCCCAG
    GAGCTGCAGC AAGAGAAAGC AACTACCATG GATTTGACTA AACGTTTGGA AAGCACTGTG
    AAGACTTGTA TAGGTACCAG AATGAAAAAT CTGGCAGCTA AGGTTGAAAT ATTGAAAGAA
    ATTAGACGTG TCAGTATCAG TGTTCGTTTT GGAAATGACC TTTCAGATGC TATACAAGTG
    TTGGATGAAG ACTGCTTTAC TATCCCAGCT TCTTTGAATG AATTAGAGCT AATTTGGGCA
    GAATATAATC TGGCTCAGGA GAAGATTCAA ACTTGTGAGA ATGTGAATGA AGGGAATATT
    TTGATTGCTG AAAGAAATGA AGTACAACAG AAGCTGTACA TGGCAGTAGA AGTTTTTATT
    CTGGAAGTTG ATGAGTTATC TCTTAATAAA CGCTTAAAAA CGTTGCAGGA TTTGTCAGCC
    TCTTTAGAAG CAGTATATGG ACAGGCCAAA GAAGGAATTA ACTCTGAAGA AATACTGGAG
    AAATTTAATG ACTGGAGGTG TAATAAAAGA GAGGAGTTCA CCAGTGTTAG AAGTGAAACA
    GAGACTTGTA TACAACGTCT TATAGCATGG TTCCAGAATA CTTTAAAGGC CTTTGATCTG
    TCTGTGGACA AATCACTGAT TTCTGATGAC ACAATTGATA ATATTGATGA AATCCTGGAG
    AACACTGAGT CAGGTGTCTG CAAGGAGCTG GAGATATCTC TGATTGAACA AGGTGATGCA
    GACAAGGAGA TCATTTTAAA AACATGTAGT CAAGTACTAC AAAAGATCCG TTCAGAGGAA
    AGCCTTATTG CCACAGTACA ATCCAAGTAC AAGGACAGTG TTGAGTTTAA GAAACAGATT
    ATTGAATGTT TAAATAAGAG CCCCAGCGTG GATCACTTGC TTTCCATTAA AAAGACACTG
    AAAAGCTTAA AGGCTCGACT GAGATGGAAA TTGGTTGAAA AGAATAATTT GGAAGAATCT
    GATGACCATG ATGGATCTGA GATTGAAAAA ATAAAACAAG AAATAACTCA GTTACGGAAT
    AGTGTCTTTG AGGGAATTTA TCATGAGCAG GAGGAATATG CGAAGCTGAG TAGCTTAGTA
    CAAAAATGGT TCCCTGAGCT TCCTCTACTT CATCCTGAAA TAGGATTACT TAAGTACATG
    AACTCTGGTG GTCTTCTTAC AATGAGCTTG GAAAGAGATC TTCTTGATGC TGAGCCTATG
    AAGGAGCTTA GCAGCAAGCG TCCTTTGATA TGTTCTGAGG TTAATGGGCA GATAATTCTT
    TTAAAGGGCT ATTCTGTGGA TGTCGACACA GAAGCCAGGG TAATTCAGAG AGCAGCTGCC
    TATCACACAG CTTGGAGAGA AGCTAAAGGA GAATCTGGGT TGCTACCACT GATATTCTTA
    TTTTTGTGTA AGTCGGATCC TATGGCTTAT CTGATGGTCC CATATTATCC TAGGGCAAAT
    CTGAATGCTG TTCAGTCCAC CATGCCTTTA ACTTCAGAAG AAGTTTTAAA GGTCATGAAA
    GGTGTAGCCC AGGGCCTTCA TACATTGCAC AAGGCTGACA TAGTTCATGG ATCACTTCAT
    CAGAACAATG TTTTTGCTTT AAACCGTCAA CATGGAATTG TTGGAGATTT TGACTTCACC
    AAATCTGTGA GTCAGCGAAC CTCAGTGAAC ATGATGGTTG GTGACTTAAG TTTGCTATCA
    CCTGAACTGA AAATGGGAAA ACCTGCTTCT CCGAGTTCTG ACTTATATGC TTATGGCTGC
    CTTTTATTGT GGCTTTCAGT TCAGAATCAG GAATTTGAAA CTAATGAGGA TGGAATCCCA
    AAGATGGATC AGTTTCATTT GGATGATAAT GTCAAATCCC TCCTCTGTAG CTTGATCTGT
    TGTAGAAGTT CAATGACTGC CGAGCAGGTT TTGAATGCTG ACTGTTTCTT GCTACCAAAG
    GGAAAATCAA TGCCAAACTC AGAGAAAGAT ACTGAATACA TCCCCAAAAA AGAGGAAGAA
    GAATTAAAGG TGAAGAACTT GGATAAAGAT AAGGAAAAGA CAAGAAATGG TGAAGCCAGC
    TTTGATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18655
    Clone ID Related Accession (Same CDS sequence) XM_005205559.3 , XM_015463068.1 , XM_005205559.4
    Accession Version XM_015463068.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3069bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein FAM221A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644725.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGTTGGCAC AGCAACGAGC GCGCAAGCGC CGTTTGGAGA CCCAGTGGCC AGAACACGTG 
    AGTTTTGCAG GAATTGTCCA AATGGAGGAA GATAAACATT GCAATAAAGT TGAAGATGTG
    GTTGGAAGTC ATGTGGAAGA TGCAGTAACA TTTTGGGCCC AGAGTATCAA TAGAAATAAG
    GATATTATGA AGATTGGTTG TTCACTGTCT GAAGTCTGTC CCCTTGCCAA TTCAGTGTTT
    GGGAATCTTG ATCCAAAGAA GATTTATGGT GGATTATTTT CTGAAGATAA ATGTTGGTAC
    AGATGCCAAG TACTGAAAGT CATCAACGAT GAAAAGTGTC TGGTGAGATA TATTGACTAT
    GGAAATACAG AAATTCTGAA TCGATCGGAT ATAGTAGAGA TTCCTTTGGA TCTGCAATTT
    TCTAGTGTTG CCAAAAAGTA TAGACTTTGG GGACTACAGA TTCCTTCTAG CCAAGAAGTT
    ACCCAGTTTG ATCAGGGCAG AACCTTTTTG GGGAGCTTGA TTTTTGAAAA GGAAATTAAA
    ATGAGAATTA AAGCTACCTA TCAAGATGGA ACAATAATTG CTCAGGCTGA GTATGGCAAT
    GTAGATATTG GGGAAGAGGT GGCTAAGAAA GGATTTGCAG AAAAATGCAG ACTCACTTCC
    AGCACTAATA TCTGTGAGGA AAAAAAATCA GATCCTGGTC AGCTTGTCCT CAGGAACCTC
    AAAAGCCCCA TTCCTTTGTG GGGGCATAGA TCAAGCCAAT CAAACTTCAC CCGGCCAAAG
    GGGCGCTTAA GTGGGAGAAT GTGTCTTGAC TTGAAGAATG AAAATGATGC AGGAAATAAT
    GTGACATTTC CAAAGGAAAA TTTGGCTGTT GGTGACTTTA ATTTAGGGTC TAATGTCAGC
    CTGGCAAAAA TTAAGCAGGA CCAGAAACTG ATTGAAGAAA ATGAAAAACT TAAAACAGAG
    AAGGAGGTTC TTCTTGAAAG GTGCAAGGCA TTAGAATTGA AAGTCGAACA GACTGCCCAG
    GAGCTGCAGC AAGAGAAAGC AACTACCATG GATTTGACTA AACGTTTGGA AAGCACTGTG
    AAGACTTGTA TAGGTACCAG AATGAAAAAT CTGGCAGCTA AGGTTGAAAT ATTGAAAGAA
    ATTAGACGTG TCAGTATCAG TGTTCGTTTT GGAAATGACC TTTCAGATGC TATACAAGTG
    TTGGATGAAG ACTGCTTTAC TATCCCAGCT TCTTTGAATG AATTAGAGCT AATTTGGGCA
    GAATATAATC TGGCTCAGGA GAAGATTCAA ACTTGTGAGA ATGTGAATGA AGGGAATATT
    TTGATTGCTG AAAGAAATGA AGTACAACAG AAGCTGTACA TGGCAGTAGA AGTTTTTATT
    CTGGAAGTTG ATGAGTTATC TCTTAATAAA CGCTTAAAAA CGTTGCAGGA TTTGTCAGCC
    TCTTTAGAAG CAGTATATGG ACAGGCCAAA GAAGGAATTA ACTCTGAAGA AATACTGGAG
    AAATTTAATG ACTGGAGGTG TAATAAAAGA GAGGAGTTCA CCAGTGTTAG AAGTGAAACA
    GAGACTTGTA TACAACGTCT TATAGCATGG TTCCAGAATA CTTTAAAGGC CTTTGATCTG
    TCTGTGGACA AATCACTGAT TTCTGATGAC ACAATTGATA ATATTGATGA AATCCTGGAG
    AACACTGAGT CAGGTGTCTG CAAGGAGCTG GAGATATCTC TGATTGAACA AGGTGATGCA
    GACAAGGAGA TCATTTTAAA AACATGTAGT CAAGTACTAC AAAAGATCCG TTCAGAGGAA
    AGCCTTATTG CCACAGTACA ATCCAAGTAC AAGGACAGTG TTGAGTTTAA GAAACAGATT
    ATTGAATGTT TAAATAAGAG CCCCAGCGTG GATCACTTGC TTTCCATTAA AAAGACACTG
    AAAAGCTTAA AGGCTCGACT GAGATGGAAA TTGGTTGAAA AGAATAATTT GGAAGAATCT
    GATGACCATG ATGGATCTGA GATTGAAAAA ATAAAACAAG AAATAACTCA GTTACGGAAT
    AGTGTCTTTG AGGGAATTTA TCATGAGCAG GAGGAATATG CGAAGCTGAG TAGCTTAGTA
    CAAAAATGGT TCCCTGAGCT TCCTCTACTT CATCCTGAAA TAGGATTACT TAAGTACATG
    AACTCTGGTG GTCTTCTTAC AATGAGCTTG GAAAGAGATC TTCTTGATGC TGAGCCTATG
    AAGGAGCTTA GCAGCAAGCG TCCTTTGATA TGTTCTGAGG TTAATGGGCA GATAATTCTT
    TTAAAGGGCT ATTCTGTGGA TGTCGACACA GAAGCCAGGG TAATTCAGAG AGCAGCTGCC
    TATCACACAG CTTGGAGAGA AGCTAAAGGA GAATCTGGGT TGCTACCACT GATATTCTTA
    TTTTTGTGTA AGTCGGATCC TATGGCTTAT CTGATGGTCC CATATTATCC TAGGGCAAAT
    CTGAATGCTG TTCAGTCCAC CATGCCTTTA ACTTCAGAAG AAGTTTTAAA GGTCATGAAA
    GGTGTAGCCC AGGGCCTTCA TACATTGCAC AAGGCTGACA TAGTTCATGG ATCACTTCAT
    CAGAACAATG TTTTTGCTTT AAACCGTCAA CATGGAATTG TTGGAGATTT TGACTTCACC
    AAATCTGTGA GTCAGCGAAC CTCAGTGAAC ATGATGGTTG GTGACTTAAG TTTGCTATCA
    CCTGAACTGA AAATGGGAAA ACCTGCTTCT CCGAGTTCTG ACTTATATGC TTATGGCTGC
    CTTTTATTGT GGCTTTCAGT TCAGAATCAG GAATTTGAAA CTAATGAGGA TGGAATCCCA
    AAGATGGATC AGTTTCATTT GGATGATAAT GTCAAATCCC TCCTCTGTAG CTTGATCTGT
    TGTAGAAGTT CAATGACTGC CGAGCAGGTT TTGAATGCTG ACTGTTTCTT GCTACCAAAG
    GGAAAATCAA TGCCAAACTC AGAGAAAGAT ACTGAATACA TCCCCAAAAA AGAGGAAGAA
    GAATTAAAGG TGAAGAACTT GGATAAAGAT AAGGAAAAGA CAAGAAATGG TGAAGCCAGC
    TTTGATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015318554.1
    CDS398..3466
    Translation

    Target ORF information:

    RefSeq Version XM_015463068.1
    Organism Bos taurus(cattle)
    Definition Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463068.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGTTGGCAC AGCAACGAGC GCGCAAGCGC CGTTTGGAGA CCCAGTGGCC AGAACACGTG 
    AGTTTTGCAG GAATTGTCCA AATGGAGGAA GATAAACATT GCAATAAAGT TGAAGATGTG
    GTTGGAAGTC ATGTGGAAGA TGCAGTAACA TTTTGGGCCC AGAGTATCAA TAGAAATAAG
    GATATTATGA AGATTGGTTG TTCACTGTCT GAAGTCTGTC CCCTTGCCAA TTCAGTGTTT
    GGGAATCTTG ATCCAAAGAA GATTTATGGT GGATTATTTT CTGAAGATAA ATGTTGGTAC
    AGATGCCAAG TACTGAAAGT CATCAACGAT GAAAAGTGTC TGGTGAGATA TATTGACTAT
    GGAAATACAG AAATTCTGAA TCGATCGGAT ATAGTAGAGA TTCCTTTGGA TCTGCAATTT
    TCTAGTGTTG CCAAAAAGTA TAGACTTTGG GGACTACAGA TTCCTTCTAG CCAAGAAGTT
    ACCCAGTTTG ATCAGGGCAG AACCTTTTTG GGGAGCTTGA TTTTTGAAAA GGAAATTAAA
    ATGAGAATTA AAGCTACCTA TCAAGATGGA ACAATAATTG CTCAGGCTGA GTATGGCAAT
    GTAGATATTG GGGAAGAGGT GGCTAAGAAA GGATTTGCAG AAAAATGCAG ACTCACTTCC
    AGCACTAATA TCTGTGAGGA AAAAAAATCA GATCCTGGTC AGCTTGTCCT CAGGAACCTC
    AAAAGCCCCA TTCCTTTGTG GGGGCATAGA TCAAGCCAAT CAAACTTCAC CCGGCCAAAG
    GGGCGCTTAA GTGGGAGAAT GTGTCTTGAC TTGAAGAATG AAAATGATGC AGGAAATAAT
    GTGACATTTC CAAAGGAAAA TTTGGCTGTT GGTGACTTTA ATTTAGGGTC TAATGTCAGC
    CTGGCAAAAA TTAAGCAGGA CCAGAAACTG ATTGAAGAAA ATGAAAAACT TAAAACAGAG
    AAGGAGGTTC TTCTTGAAAG GTGCAAGGCA TTAGAATTGA AAGTCGAACA GACTGCCCAG
    GAGCTGCAGC AAGAGAAAGC AACTACCATG GATTTGACTA AACGTTTGGA AAGCACTGTG
    AAGACTTGTA TAGGTACCAG AATGAAAAAT CTGGCAGCTA AGGTTGAAAT ATTGAAAGAA
    ATTAGACGTG TCAGTATCAG TGTTCGTTTT GGAAATGACC TTTCAGATGC TATACAAGTG
    TTGGATGAAG ACTGCTTTAC TATCCCAGCT TCTTTGAATG AATTAGAGCT AATTTGGGCA
    GAATATAATC TGGCTCAGGA GAAGATTCAA ACTTGTGAGA ATGTGAATGA AGGGAATATT
    TTGATTGCTG AAAGAAATGA AGTACAACAG AAGCTGTACA TGGCAGTAGA AGTTTTTATT
    CTGGAAGTTG ATGAGTTATC TCTTAATAAA CGCTTAAAAA CGTTGCAGGA TTTGTCAGCC
    TCTTTAGAAG CAGTATATGG ACAGGCCAAA GAAGGAATTA ACTCTGAAGA AATACTGGAG
    AAATTTAATG ACTGGAGGTG TAATAAAAGA GAGGAGTTCA CCAGTGTTAG AAGTGAAACA
    GAGACTTGTA TACAACGTCT TATAGCATGG TTCCAGAATA CTTTAAAGGC CTTTGATCTG
    TCTGTGGACA AATCACTGAT TTCTGATGAC ACAATTGATA ATATTGATGA AATCCTGGAG
    AACACTGAGT CAGGTGTCTG CAAGGAGCTG GAGATATCTC TGATTGAACA AGGTGATGCA
    GACAAGGAGA TCATTTTAAA AACATGTAGT CAAGTACTAC AAAAGATCCG TTCAGAGGAA
    AGCCTTATTG CCACAGTACA ATCCAAGTAC AAGGACAGTG TTGAGTTTAA GAAACAGATT
    ATTGAATGTT TAAATAAGAG CCCCAGCGTG GATCACTTGC TTTCCATTAA AAAGACACTG
    AAAAGCTTAA AGGCTCGACT GAGATGGAAA TTGGTTGAAA AGAATAATTT GGAAGAATCT
    GATGACCATG ATGGATCTGA GATTGAAAAA ATAAAACAAG AAATAACTCA GTTACGGAAT
    AGTGTCTTTG AGGGAATTTA TCATGAGCAG GAGGAATATG CGAAGCTGAG TAGCTTAGTA
    CAAAAATGGT TCCCTGAGCT TCCTCTACTT CATCCTGAAA TAGGATTACT TAAGTACATG
    AACTCTGGTG GTCTTCTTAC AATGAGCTTG GAAAGAGATC TTCTTGATGC TGAGCCTATG
    AAGGAGCTTA GCAGCAAGCG TCCTTTGATA TGTTCTGAGG TTAATGGGCA GATAATTCTT
    TTAAAGGGCT ATTCTGTGGA TGTCGACACA GAAGCCAGGG TAATTCAGAG AGCAGCTGCC
    TATCACACAG CTTGGAGAGA AGCTAAAGGA GAATCTGGGT TGCTACCACT GATATTCTTA
    TTTTTGTGTA AGTCGGATCC TATGGCTTAT CTGATGGTCC CATATTATCC TAGGGCAAAT
    CTGAATGCTG TTCAGTCCAC CATGCCTTTA ACTTCAGAAG AAGTTTTAAA GGTCATGAAA
    GGTGTAGCCC AGGGCCTTCA TACATTGCAC AAGGCTGACA TAGTTCATGG ATCACTTCAT
    CAGAACAATG TTTTTGCTTT AAACCGTCAA CATGGAATTG TTGGAGATTT TGACTTCACC
    AAATCTGTGA GTCAGCGAAC CTCAGTGAAC ATGATGGTTG GTGACTTAAG TTTGCTATCA
    CCTGAACTGA AAATGGGAAA ACCTGCTTCT CCGAGTTCTG ACTTATATGC TTATGGCTGC
    CTTTTATTGT GGCTTTCAGT TCAGAATCAG GAATTTGAAA CTAATGAGGA TGGAATCCCA
    AAGATGGATC AGTTTCATTT GGATGATAAT GTCAAATCCC TCCTCTGTAG CTTGATCTGT
    TGTAGAAGTT CAATGACTGC CGAGCAGGTT TTGAATGCTG ACTGTTTCTT GCTACCAAAG
    GGAAAATCAA TGCCAAACTC AGAGAAAGAT ACTGAATACA TCCCCAAAAA AGAGGAAGAA
    GAATTAAAGG TGAAGAACTT GGATAAAGAT AAGGAAAAGA CAAGAAATGG TGAAGCCAGC
    TTTGATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18655
    Clone ID Related Accession (Same CDS sequence) XM_005205559.3 , XM_015463068.1 , XM_005205559.4
    Accession Version XM_005205559.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3069bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein FAM221A isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005205559.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGTTGGCAC AGCAACGAGC GCGCAAGCGC CGTTTGGAGA CCCAGTGGCC AGAACACGTG 
    AGTTTTGCAG GAATTGTCCA AATGGAGGAA GATAAACATT GCAATAAAGT TGAAGATGTG
    GTTGGAAGTC ATGTGGAAGA TGCAGTAACA TTTTGGGCCC AGAGTATCAA TAGAAATAAG
    GATATTATGA AGATTGGTTG TTCACTGTCT GAAGTCTGTC CCCTTGCCAA TTCAGTGTTT
    GGGAATCTTG ATCCAAAGAA GATTTATGGT GGATTATTTT CTGAAGATAA ATGTTGGTAC
    AGATGCCAAG TACTGAAAGT CATCAACGAT GAAAAGTGTC TGGTGAGATA TATTGACTAT
    GGAAATACAG AAATTCTGAA TCGATCGGAT ATAGTAGAGA TTCCTTTGGA TCTGCAATTT
    TCTAGTGTTG CCAAAAAGTA TAGACTTTGG GGACTACAGA TTCCTTCTAG CCAAGAAGTT
    ACCCAGTTTG ATCAGGGCAG AACCTTTTTG GGGAGCTTGA TTTTTGAAAA GGAAATTAAA
    ATGAGAATTA AAGCTACCTA TCAAGATGGA ACAATAATTG CTCAGGCTGA GTATGGCAAT
    GTAGATATTG GGGAAGAGGT GGCTAAGAAA GGATTTGCAG AAAAATGCAG ACTCACTTCC
    AGCACTAATA TCTGTGAGGA AAAAAAATCA GATCCTGGTC AGCTTGTCCT CAGGAACCTC
    AAAAGCCCCA TTCCTTTGTG GGGGCATAGA TCAAGCCAAT CAAACTTCAC CCGGCCAAAG
    GGGCGCTTAA GTGGGAGAAT GTGTCTTGAC TTGAAGAATG AAAATGATGC AGGAAATAAT
    GTGACATTTC CAAAGGAAAA TTTGGCTGTT GGTGACTTTA ATTTAGGGTC TAATGTCAGC
    CTGGCAAAAA TTAAGCAGGA CCAGAAACTG ATTGAAGAAA ATGAAAAACT TAAAACAGAG
    AAGGAGGTTC TTCTTGAAAG GTGCAAGGCA TTAGAATTGA AAGTCGAACA GACTGCCCAG
    GAGCTGCAGC AAGAGAAAGC AACTACCATG GATTTGACTA AACGTTTGGA AAGCACTGTG
    AAGACTTGTA TAGGTACCAG AATGAAAAAT CTGGCAGCTA AGGTTGAAAT ATTGAAAGAA
    ATTAGACGTG TCAGTATCAG TGTTCGTTTT GGAAATGACC TTTCAGATGC TATACAAGTG
    TTGGATGAAG ACTGCTTTAC TATCCCAGCT TCTTTGAATG AATTAGAGCT AATTTGGGCA
    GAATATAATC TGGCTCAGGA GAAGATTCAA ACTTGTGAGA ATGTGAATGA AGGGAATATT
    TTGATTGCTG AAAGAAATGA AGTACAACAG AAGCTGTACA TGGCAGTAGA AGTTTTTATT
    CTGGAAGTTG ATGAGTTATC TCTTAATAAA CGCTTAAAAA CGTTGCAGGA TTTGTCAGCC
    TCTTTAGAAG CAGTATATGG ACAGGCCAAA GAAGGAATTA ACTCTGAAGA AATACTGGAG
    AAATTTAATG ACTGGAGGTG TAATAAAAGA GAGGAGTTCA CCAGTGTTAG AAGTGAAACA
    GAGACTTGTA TACAACGTCT TATAGCATGG TTCCAGAATA CTTTAAAGGC CTTTGATCTG
    TCTGTGGACA AATCACTGAT TTCTGATGAC ACAATTGATA ATATTGATGA AATCCTGGAG
    AACACTGAGT CAGGTGTCTG CAAGGAGCTG GAGATATCTC TGATTGAACA AGGTGATGCA
    GACAAGGAGA TCATTTTAAA AACATGTAGT CAAGTACTAC AAAAGATCCG TTCAGAGGAA
    AGCCTTATTG CCACAGTACA ATCCAAGTAC AAGGACAGTG TTGAGTTTAA GAAACAGATT
    ATTGAATGTT TAAATAAGAG CCCCAGCGTG GATCACTTGC TTTCCATTAA AAAGACACTG
    AAAAGCTTAA AGGCTCGACT GAGATGGAAA TTGGTTGAAA AGAATAATTT GGAAGAATCT
    GATGACCATG ATGGATCTGA GATTGAAAAA ATAAAACAAG AAATAACTCA GTTACGGAAT
    AGTGTCTTTG AGGGAATTTA TCATGAGCAG GAGGAATATG CGAAGCTGAG TAGCTTAGTA
    CAAAAATGGT TCCCTGAGCT TCCTCTACTT CATCCTGAAA TAGGATTACT TAAGTACATG
    AACTCTGGTG GTCTTCTTAC AATGAGCTTG GAAAGAGATC TTCTTGATGC TGAGCCTATG
    AAGGAGCTTA GCAGCAAGCG TCCTTTGATA TGTTCTGAGG TTAATGGGCA GATAATTCTT
    TTAAAGGGCT ATTCTGTGGA TGTCGACACA GAAGCCAGGG TAATTCAGAG AGCAGCTGCC
    TATCACACAG CTTGGAGAGA AGCTAAAGGA GAATCTGGGT TGCTACCACT GATATTCTTA
    TTTTTGTGTA AGTCGGATCC TATGGCTTAT CTGATGGTCC CATATTATCC TAGGGCAAAT
    CTGAATGCTG TTCAGTCCAC CATGCCTTTA ACTTCAGAAG AAGTTTTAAA GGTCATGAAA
    GGTGTAGCCC AGGGCCTTCA TACATTGCAC AAGGCTGACA TAGTTCATGG ATCACTTCAT
    CAGAACAATG TTTTTGCTTT AAACCGTCAA CATGGAATTG TTGGAGATTT TGACTTCACC
    AAATCTGTGA GTCAGCGAAC CTCAGTGAAC ATGATGGTTG GTGACTTAAG TTTGCTATCA
    CCTGAACTGA AAATGGGAAA ACCTGCTTCT CCGAGTTCTG ACTTATATGC TTATGGCTGC
    CTTTTATTGT GGCTTTCAGT TCAGAATCAG GAATTTGAAA CTAATGAGGA TGGAATCCCA
    AAGATGGATC AGTTTCATTT GGATGATAAT GTCAAATCCC TCCTCTGTAG CTTGATCTGT
    TGTAGAAGTT CAATGACTGC CGAGCAGGTT TTGAATGCTG ACTGTTTCTT GCTACCAAAG
    GGAAAATCAA TGCCAAACTC AGAGAAAGAT ACTGAATACA TCCCCAAAAA AGAGGAAGAA
    GAATTAAAGG TGAAGAACTT GGATAAAGAT AAGGAAAAGA CAAGAAATGG TGAAGCCAGC
    TTTGATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005205616.1
    CDS317..3385
    Translation

    Target ORF information:

    RefSeq Version XM_005205559.4
    Organism Bos taurus(cattle)
    Definition Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005205559.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGTTGGCAC AGCAACGAGC GCGCAAGCGC CGTTTGGAGA CCCAGTGGCC AGAACACGTG 
    AGTTTTGCAG GAATTGTCCA AATGGAGGAA GATAAACATT GCAATAAAGT TGAAGATGTG
    GTTGGAAGTC ATGTGGAAGA TGCAGTAACA TTTTGGGCCC AGAGTATCAA TAGAAATAAG
    GATATTATGA AGATTGGTTG TTCACTGTCT GAAGTCTGTC CCCTTGCCAA TTCAGTGTTT
    GGGAATCTTG ATCCAAAGAA GATTTATGGT GGATTATTTT CTGAAGATAA ATGTTGGTAC
    AGATGCCAAG TACTGAAAGT CATCAACGAT GAAAAGTGTC TGGTGAGATA TATTGACTAT
    GGAAATACAG AAATTCTGAA TCGATCGGAT ATAGTAGAGA TTCCTTTGGA TCTGCAATTT
    TCTAGTGTTG CCAAAAAGTA TAGACTTTGG GGACTACAGA TTCCTTCTAG CCAAGAAGTT
    ACCCAGTTTG ATCAGGGCAG AACCTTTTTG GGGAGCTTGA TTTTTGAAAA GGAAATTAAA
    ATGAGAATTA AAGCTACCTA TCAAGATGGA ACAATAATTG CTCAGGCTGA GTATGGCAAT
    GTAGATATTG GGGAAGAGGT GGCTAAGAAA GGATTTGCAG AAAAATGCAG ACTCACTTCC
    AGCACTAATA TCTGTGAGGA AAAAAAATCA GATCCTGGTC AGCTTGTCCT CAGGAACCTC
    AAAAGCCCCA TTCCTTTGTG GGGGCATAGA TCAAGCCAAT CAAACTTCAC CCGGCCAAAG
    GGGCGCTTAA GTGGGAGAAT GTGTCTTGAC TTGAAGAATG AAAATGATGC AGGAAATAAT
    GTGACATTTC CAAAGGAAAA TTTGGCTGTT GGTGACTTTA ATTTAGGGTC TAATGTCAGC
    CTGGCAAAAA TTAAGCAGGA CCAGAAACTG ATTGAAGAAA ATGAAAAACT TAAAACAGAG
    AAGGAGGTTC TTCTTGAAAG GTGCAAGGCA TTAGAATTGA AAGTCGAACA GACTGCCCAG
    GAGCTGCAGC AAGAGAAAGC AACTACCATG GATTTGACTA AACGTTTGGA AAGCACTGTG
    AAGACTTGTA TAGGTACCAG AATGAAAAAT CTGGCAGCTA AGGTTGAAAT ATTGAAAGAA
    ATTAGACGTG TCAGTATCAG TGTTCGTTTT GGAAATGACC TTTCAGATGC TATACAAGTG
    TTGGATGAAG ACTGCTTTAC TATCCCAGCT TCTTTGAATG AATTAGAGCT AATTTGGGCA
    GAATATAATC TGGCTCAGGA GAAGATTCAA ACTTGTGAGA ATGTGAATGA AGGGAATATT
    TTGATTGCTG AAAGAAATGA AGTACAACAG AAGCTGTACA TGGCAGTAGA AGTTTTTATT
    CTGGAAGTTG ATGAGTTATC TCTTAATAAA CGCTTAAAAA CGTTGCAGGA TTTGTCAGCC
    TCTTTAGAAG CAGTATATGG ACAGGCCAAA GAAGGAATTA ACTCTGAAGA AATACTGGAG
    AAATTTAATG ACTGGAGGTG TAATAAAAGA GAGGAGTTCA CCAGTGTTAG AAGTGAAACA
    GAGACTTGTA TACAACGTCT TATAGCATGG TTCCAGAATA CTTTAAAGGC CTTTGATCTG
    TCTGTGGACA AATCACTGAT TTCTGATGAC ACAATTGATA ATATTGATGA AATCCTGGAG
    AACACTGAGT CAGGTGTCTG CAAGGAGCTG GAGATATCTC TGATTGAACA AGGTGATGCA
    GACAAGGAGA TCATTTTAAA AACATGTAGT CAAGTACTAC AAAAGATCCG TTCAGAGGAA
    AGCCTTATTG CCACAGTACA ATCCAAGTAC AAGGACAGTG TTGAGTTTAA GAAACAGATT
    ATTGAATGTT TAAATAAGAG CCCCAGCGTG GATCACTTGC TTTCCATTAA AAAGACACTG
    AAAAGCTTAA AGGCTCGACT GAGATGGAAA TTGGTTGAAA AGAATAATTT GGAAGAATCT
    GATGACCATG ATGGATCTGA GATTGAAAAA ATAAAACAAG AAATAACTCA GTTACGGAAT
    AGTGTCTTTG AGGGAATTTA TCATGAGCAG GAGGAATATG CGAAGCTGAG TAGCTTAGTA
    CAAAAATGGT TCCCTGAGCT TCCTCTACTT CATCCTGAAA TAGGATTACT TAAGTACATG
    AACTCTGGTG GTCTTCTTAC AATGAGCTTG GAAAGAGATC TTCTTGATGC TGAGCCTATG
    AAGGAGCTTA GCAGCAAGCG TCCTTTGATA TGTTCTGAGG TTAATGGGCA GATAATTCTT
    TTAAAGGGCT ATTCTGTGGA TGTCGACACA GAAGCCAGGG TAATTCAGAG AGCAGCTGCC
    TATCACACAG CTTGGAGAGA AGCTAAAGGA GAATCTGGGT TGCTACCACT GATATTCTTA
    TTTTTGTGTA AGTCGGATCC TATGGCTTAT CTGATGGTCC CATATTATCC TAGGGCAAAT
    CTGAATGCTG TTCAGTCCAC CATGCCTTTA ACTTCAGAAG AAGTTTTAAA GGTCATGAAA
    GGTGTAGCCC AGGGCCTTCA TACATTGCAC AAGGCTGACA TAGTTCATGG ATCACTTCAT
    CAGAACAATG TTTTTGCTTT AAACCGTCAA CATGGAATTG TTGGAGATTT TGACTTCACC
    AAATCTGTGA GTCAGCGAAC CTCAGTGAAC ATGATGGTTG GTGACTTAAG TTTGCTATCA
    CCTGAACTGA AAATGGGAAA ACCTGCTTCT CCGAGTTCTG ACTTATATGC TTATGGCTGC
    CTTTTATTGT GGCTTTCAGT TCAGAATCAG GAATTTGAAA CTAATGAGGA TGGAATCCCA
    AAGATGGATC AGTTTCATTT GGATGATAAT GTCAAATCCC TCCTCTGTAG CTTGATCTGT
    TGTAGAAGTT CAATGACTGC CGAGCAGGTT TTGAATGCTG ACTGTTTCTT GCTACCAAAG
    GGAAAATCAA TGCCAAACTC AGAGAAAGAT ACTGAATACA TCCCCAAAAA AGAGGAAGAA
    GAATTAAAGG TGAAGAACTT GGATAAAGAT AAGGAAAAGA CAAGAAATGG TGAAGCCAGC
    TTTGATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18656
    Clone ID Related Accession (Same CDS sequence) XM_015463069.1 , XM_005205560.3 , XM_005205560.4
    Accession Version XM_015463069.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3057bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein FAM221A isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644725.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTGGGGCC TGGGGCATTC TTCTAGAACT TCTGTGGCAG GAAGTGCGAG TTTTGCAGGA 
    ATTGTCCAAA TGGAGGAAGA TAAACATTGC AATAAAGTTG AAGATGTGGT TGGAAGTCAT
    GTGGAAGATG CAGTAACATT TTGGGCCCAG AGTATCAATA GAAATAAGGA TATTATGAAG
    ATTGGTTGTT CACTGTCTGA AGTCTGTCCC CTTGCCAATT CAGTGTTTGG GAATCTTGAT
    CCAAAGAAGA TTTATGGTGG ATTATTTTCT GAAGATAAAT GTTGGTACAG ATGCCAAGTA
    CTGAAAGTCA TCAACGATGA AAAGTGTCTG GTGAGATATA TTGACTATGG AAATACAGAA
    ATTCTGAATC GATCGGATAT AGTAGAGATT CCTTTGGATC TGCAATTTTC TAGTGTTGCC
    AAAAAGTATA GACTTTGGGG ACTACAGATT CCTTCTAGCC AAGAAGTTAC CCAGTTTGAT
    CAGGGCAGAA CCTTTTTGGG GAGCTTGATT TTTGAAAAGG AAATTAAAAT GAGAATTAAA
    GCTACCTATC AAGATGGAAC AATAATTGCT CAGGCTGAGT ATGGCAATGT AGATATTGGG
    GAAGAGGTGG CTAAGAAAGG ATTTGCAGAA AAATGCAGAC TCACTTCCAG CACTAATATC
    TGTGAGGAAA AAAAATCAGA TCCTGGTCAG CTTGTCCTCA GGAACCTCAA AAGCCCCATT
    CCTTTGTGGG GGCATAGATC AAGCCAATCA AACTTCACCC GGCCAAAGGG GCGCTTAAGT
    GGGAGAATGT GTCTTGACTT GAAGAATGAA AATGATGCAG GAAATAATGT GACATTTCCA
    AAGGAAAATT TGGCTGTTGG TGACTTTAAT TTAGGGTCTA ATGTCAGCCT GGCAAAAATT
    AAGCAGGACC AGAAACTGAT TGAAGAAAAT GAAAAACTTA AAACAGAGAA GGAGGTTCTT
    CTTGAAAGGT GCAAGGCATT AGAATTGAAA GTCGAACAGA CTGCCCAGGA GCTGCAGCAA
    GAGAAAGCAA CTACCATGGA TTTGACTAAA CGTTTGGAAA GCACTGTGAA GACTTGTATA
    GGTACCAGAA TGAAAAATCT GGCAGCTAAG GTTGAAATAT TGAAAGAAAT TAGACGTGTC
    AGTATCAGTG TTCGTTTTGG AAATGACCTT TCAGATGCTA TACAAGTGTT GGATGAAGAC
    TGCTTTACTA TCCCAGCTTC TTTGAATGAA TTAGAGCTAA TTTGGGCAGA ATATAATCTG
    GCTCAGGAGA AGATTCAAAC TTGTGAGAAT GTGAATGAAG GGAATATTTT GATTGCTGAA
    AGAAATGAAG TACAACAGAA GCTGTACATG GCAGTAGAAG TTTTTATTCT GGAAGTTGAT
    GAGTTATCTC TTAATAAACG CTTAAAAACG TTGCAGGATT TGTCAGCCTC TTTAGAAGCA
    GTATATGGAC AGGCCAAAGA AGGAATTAAC TCTGAAGAAA TACTGGAGAA ATTTAATGAC
    TGGAGGTGTA ATAAAAGAGA GGAGTTCACC AGTGTTAGAA GTGAAACAGA GACTTGTATA
    CAACGTCTTA TAGCATGGTT CCAGAATACT TTAAAGGCCT TTGATCTGTC TGTGGACAAA
    TCACTGATTT CTGATGACAC AATTGATAAT ATTGATGAAA TCCTGGAGAA CACTGAGTCA
    GGTGTCTGCA AGGAGCTGGA GATATCTCTG ATTGAACAAG GTGATGCAGA CAAGGAGATC
    ATTTTAAAAA CATGTAGTCA AGTACTACAA AAGATCCGTT CAGAGGAAAG CCTTATTGCC
    ACAGTACAAT CCAAGTACAA GGACAGTGTT GAGTTTAAGA AACAGATTAT TGAATGTTTA
    AATAAGAGCC CCAGCGTGGA TCACTTGCTT TCCATTAAAA AGACACTGAA AAGCTTAAAG
    GCTCGACTGA GATGGAAATT GGTTGAAAAG AATAATTTGG AAGAATCTGA TGACCATGAT
    GGATCTGAGA TTGAAAAAAT AAAACAAGAA ATAACTCAGT TACGGAATAG TGTCTTTGAG
    GGAATTTATC ATGAGCAGGA GGAATATGCG AAGCTGAGTA GCTTAGTACA AAAATGGTTC
    CCTGAGCTTC CTCTACTTCA TCCTGAAATA GGATTACTTA AGTACATGAA CTCTGGTGGT
    CTTCTTACAA TGAGCTTGGA AAGAGATCTT CTTGATGCTG AGCCTATGAA GGAGCTTAGC
    AGCAAGCGTC CTTTGATATG TTCTGAGGTT AATGGGCAGA TAATTCTTTT AAAGGGCTAT
    TCTGTGGATG TCGACACAGA AGCCAGGGTA ATTCAGAGAG CAGCTGCCTA TCACACAGCT
    TGGAGAGAAG CTAAAGGAGA ATCTGGGTTG CTACCACTGA TATTCTTATT TTTGTGTAAG
    TCGGATCCTA TGGCTTATCT GATGGTCCCA TATTATCCTA GGGCAAATCT GAATGCTGTT
    CAGTCCACCA TGCCTTTAAC TTCAGAAGAA GTTTTAAAGG TCATGAAAGG TGTAGCCCAG
    GGCCTTCATA CATTGCACAA GGCTGACATA GTTCATGGAT CACTTCATCA GAACAATGTT
    TTTGCTTTAA ACCGTCAACA TGGAATTGTT GGAGATTTTG ACTTCACCAA ATCTGTGAGT
    CAGCGAACCT CAGTGAACAT GATGGTTGGT GACTTAAGTT TGCTATCACC TGAACTGAAA
    ATGGGAAAAC CTGCTTCTCC GAGTTCTGAC TTATATGCTT ATGGCTGCCT TTTATTGTGG
    CTTTCAGTTC AGAATCAGGA ATTTGAAACT AATGAGGATG GAATCCCAAA GATGGATCAG
    TTTCATTTGG ATGATAATGT CAAATCCCTC CTCTGTAGCT TGATCTGTTG TAGAAGTTCA
    ATGACTGCCG AGCAGGTTTT GAATGCTGAC TGTTTCTTGC TACCAAAGGG AAAATCAATG
    CCAAACTCAG AGAAAGATAC TGAATACATC CCCAAAAAAG AGGAAGAAGA ATTAAAGGTG
    AAGAACTTGG ATAAAGATAA GGAAAAGACA AGAAATGGTG AAGCCAGCTT TGATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015318555.1
    CDS21..3077
    Translation

    Target ORF information:

    RefSeq Version XM_015463069.1
    Organism Bos taurus(cattle)
    Definition Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463069.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTGGGGCC TGGGGCATTC TTCTAGAACT TCTGTGGCAG GAAGTGCGAG TTTTGCAGGA 
    ATTGTCCAAA TGGAGGAAGA TAAACATTGC AATAAAGTTG AAGATGTGGT TGGAAGTCAT
    GTGGAAGATG CAGTAACATT TTGGGCCCAG AGTATCAATA GAAATAAGGA TATTATGAAG
    ATTGGTTGTT CACTGTCTGA AGTCTGTCCC CTTGCCAATT CAGTGTTTGG GAATCTTGAT
    CCAAAGAAGA TTTATGGTGG ATTATTTTCT GAAGATAAAT GTTGGTACAG ATGCCAAGTA
    CTGAAAGTCA TCAACGATGA AAAGTGTCTG GTGAGATATA TTGACTATGG AAATACAGAA
    ATTCTGAATC GATCGGATAT AGTAGAGATT CCTTTGGATC TGCAATTTTC TAGTGTTGCC
    AAAAAGTATA GACTTTGGGG ACTACAGATT CCTTCTAGCC AAGAAGTTAC CCAGTTTGAT
    CAGGGCAGAA CCTTTTTGGG GAGCTTGATT TTTGAAAAGG AAATTAAAAT GAGAATTAAA
    GCTACCTATC AAGATGGAAC AATAATTGCT CAGGCTGAGT ATGGCAATGT AGATATTGGG
    GAAGAGGTGG CTAAGAAAGG ATTTGCAGAA AAATGCAGAC TCACTTCCAG CACTAATATC
    TGTGAGGAAA AAAAATCAGA TCCTGGTCAG CTTGTCCTCA GGAACCTCAA AAGCCCCATT
    CCTTTGTGGG GGCATAGATC AAGCCAATCA AACTTCACCC GGCCAAAGGG GCGCTTAAGT
    GGGAGAATGT GTCTTGACTT GAAGAATGAA AATGATGCAG GAAATAATGT GACATTTCCA
    AAGGAAAATT TGGCTGTTGG TGACTTTAAT TTAGGGTCTA ATGTCAGCCT GGCAAAAATT
    AAGCAGGACC AGAAACTGAT TGAAGAAAAT GAAAAACTTA AAACAGAGAA GGAGGTTCTT
    CTTGAAAGGT GCAAGGCATT AGAATTGAAA GTCGAACAGA CTGCCCAGGA GCTGCAGCAA
    GAGAAAGCAA CTACCATGGA TTTGACTAAA CGTTTGGAAA GCACTGTGAA GACTTGTATA
    GGTACCAGAA TGAAAAATCT GGCAGCTAAG GTTGAAATAT TGAAAGAAAT TAGACGTGTC
    AGTATCAGTG TTCGTTTTGG AAATGACCTT TCAGATGCTA TACAAGTGTT GGATGAAGAC
    TGCTTTACTA TCCCAGCTTC TTTGAATGAA TTAGAGCTAA TTTGGGCAGA ATATAATCTG
    GCTCAGGAGA AGATTCAAAC TTGTGAGAAT GTGAATGAAG GGAATATTTT GATTGCTGAA
    AGAAATGAAG TACAACAGAA GCTGTACATG GCAGTAGAAG TTTTTATTCT GGAAGTTGAT
    GAGTTATCTC TTAATAAACG CTTAAAAACG TTGCAGGATT TGTCAGCCTC TTTAGAAGCA
    GTATATGGAC AGGCCAAAGA AGGAATTAAC TCTGAAGAAA TACTGGAGAA ATTTAATGAC
    TGGAGGTGTA ATAAAAGAGA GGAGTTCACC AGTGTTAGAA GTGAAACAGA GACTTGTATA
    CAACGTCTTA TAGCATGGTT CCAGAATACT TTAAAGGCCT TTGATCTGTC TGTGGACAAA
    TCACTGATTT CTGATGACAC AATTGATAAT ATTGATGAAA TCCTGGAGAA CACTGAGTCA
    GGTGTCTGCA AGGAGCTGGA GATATCTCTG ATTGAACAAG GTGATGCAGA CAAGGAGATC
    ATTTTAAAAA CATGTAGTCA AGTACTACAA AAGATCCGTT CAGAGGAAAG CCTTATTGCC
    ACAGTACAAT CCAAGTACAA GGACAGTGTT GAGTTTAAGA AACAGATTAT TGAATGTTTA
    AATAAGAGCC CCAGCGTGGA TCACTTGCTT TCCATTAAAA AGACACTGAA AAGCTTAAAG
    GCTCGACTGA GATGGAAATT GGTTGAAAAG AATAATTTGG AAGAATCTGA TGACCATGAT
    GGATCTGAGA TTGAAAAAAT AAAACAAGAA ATAACTCAGT TACGGAATAG TGTCTTTGAG
    GGAATTTATC ATGAGCAGGA GGAATATGCG AAGCTGAGTA GCTTAGTACA AAAATGGTTC
    CCTGAGCTTC CTCTACTTCA TCCTGAAATA GGATTACTTA AGTACATGAA CTCTGGTGGT
    CTTCTTACAA TGAGCTTGGA AAGAGATCTT CTTGATGCTG AGCCTATGAA GGAGCTTAGC
    AGCAAGCGTC CTTTGATATG TTCTGAGGTT AATGGGCAGA TAATTCTTTT AAAGGGCTAT
    TCTGTGGATG TCGACACAGA AGCCAGGGTA ATTCAGAGAG CAGCTGCCTA TCACACAGCT
    TGGAGAGAAG CTAAAGGAGA ATCTGGGTTG CTACCACTGA TATTCTTATT TTTGTGTAAG
    TCGGATCCTA TGGCTTATCT GATGGTCCCA TATTATCCTA GGGCAAATCT GAATGCTGTT
    CAGTCCACCA TGCCTTTAAC TTCAGAAGAA GTTTTAAAGG TCATGAAAGG TGTAGCCCAG
    GGCCTTCATA CATTGCACAA GGCTGACATA GTTCATGGAT CACTTCATCA GAACAATGTT
    TTTGCTTTAA ACCGTCAACA TGGAATTGTT GGAGATTTTG ACTTCACCAA ATCTGTGAGT
    CAGCGAACCT CAGTGAACAT GATGGTTGGT GACTTAAGTT TGCTATCACC TGAACTGAAA
    ATGGGAAAAC CTGCTTCTCC GAGTTCTGAC TTATATGCTT ATGGCTGCCT TTTATTGTGG
    CTTTCAGTTC AGAATCAGGA ATTTGAAACT AATGAGGATG GAATCCCAAA GATGGATCAG
    TTTCATTTGG ATGATAATGT CAAATCCCTC CTCTGTAGCT TGATCTGTTG TAGAAGTTCA
    ATGACTGCCG AGCAGGTTTT GAATGCTGAC TGTTTCTTGC TACCAAAGGG AAAATCAATG
    CCAAACTCAG AGAAAGATAC TGAATACATC CCCAAAAAAG AGGAAGAAGA ATTAAAGGTG
    AAGAACTTGG ATAAAGATAA GGAAAAGACA AGAAATGGTG AAGCCAGCTT TGATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18656
    Clone ID Related Accession (Same CDS sequence) XM_015463069.1 , XM_005205560.3 , XM_005205560.4
    Accession Version XM_005205560.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3057bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein FAM221A isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103900.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005205560.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTGGGGCC TGGGGCATTC TTCTAGAACT TCTGTGGCAG GAAGTGCGAG TTTTGCAGGA 
    ATTGTCCAAA TGGAGGAAGA TAAACATTGC AATAAAGTTG AAGATGTGGT TGGAAGTCAT
    GTGGAAGATG CAGTAACATT TTGGGCCCAG AGTATCAATA GAAATAAGGA TATTATGAAG
    ATTGGTTGTT CACTGTCTGA AGTCTGTCCC CTTGCCAATT CAGTGTTTGG GAATCTTGAT
    CCAAAGAAGA TTTATGGTGG ATTATTTTCT GAAGATAAAT GTTGGTACAG ATGCCAAGTA
    CTGAAAGTCA TCAACGATGA AAAGTGTCTG GTGAGATATA TTGACTATGG AAATACAGAA
    ATTCTGAATC GATCGGATAT AGTAGAGATT CCTTTGGATC TGCAATTTTC TAGTGTTGCC
    AAAAAGTATA GACTTTGGGG ACTACAGATT CCTTCTAGCC AAGAAGTTAC CCAGTTTGAT
    CAGGGCAGAA CCTTTTTGGG GAGCTTGATT TTTGAAAAGG AAATTAAAAT GAGAATTAAA
    GCTACCTATC AAGATGGAAC AATAATTGCT CAGGCTGAGT ATGGCAATGT AGATATTGGG
    GAAGAGGTGG CTAAGAAAGG ATTTGCAGAA AAATGCAGAC TCACTTCCAG CACTAATATC
    TGTGAGGAAA AAAAATCAGA TCCTGGTCAG CTTGTCCTCA GGAACCTCAA AAGCCCCATT
    CCTTTGTGGG GGCATAGATC AAGCCAATCA AACTTCACCC GGCCAAAGGG GCGCTTAAGT
    GGGAGAATGT GTCTTGACTT GAAGAATGAA AATGATGCAG GAAATAATGT GACATTTCCA
    AAGGAAAATT TGGCTGTTGG TGACTTTAAT TTAGGGTCTA ATGTCAGCCT GGCAAAAATT
    AAGCAGGACC AGAAACTGAT TGAAGAAAAT GAAAAACTTA AAACAGAGAA GGAGGTTCTT
    CTTGAAAGGT GCAAGGCATT AGAATTGAAA GTCGAACAGA CTGCCCAGGA GCTGCAGCAA
    GAGAAAGCAA CTACCATGGA TTTGACTAAA CGTTTGGAAA GCACTGTGAA GACTTGTATA
    GGTACCAGAA TGAAAAATCT GGCAGCTAAG GTTGAAATAT TGAAAGAAAT TAGACGTGTC
    AGTATCAGTG TTCGTTTTGG AAATGACCTT TCAGATGCTA TACAAGTGTT GGATGAAGAC
    TGCTTTACTA TCCCAGCTTC TTTGAATGAA TTAGAGCTAA TTTGGGCAGA ATATAATCTG
    GCTCAGGAGA AGATTCAAAC TTGTGAGAAT GTGAATGAAG GGAATATTTT GATTGCTGAA
    AGAAATGAAG TACAACAGAA GCTGTACATG GCAGTAGAAG TTTTTATTCT GGAAGTTGAT
    GAGTTATCTC TTAATAAACG CTTAAAAACG TTGCAGGATT TGTCAGCCTC TTTAGAAGCA
    GTATATGGAC AGGCCAAAGA AGGAATTAAC TCTGAAGAAA TACTGGAGAA ATTTAATGAC
    TGGAGGTGTA ATAAAAGAGA GGAGTTCACC AGTGTTAGAA GTGAAACAGA GACTTGTATA
    CAACGTCTTA TAGCATGGTT CCAGAATACT TTAAAGGCCT TTGATCTGTC TGTGGACAAA
    TCACTGATTT CTGATGACAC AATTGATAAT ATTGATGAAA TCCTGGAGAA CACTGAGTCA
    GGTGTCTGCA AGGAGCTGGA GATATCTCTG ATTGAACAAG GTGATGCAGA CAAGGAGATC
    ATTTTAAAAA CATGTAGTCA AGTACTACAA AAGATCCGTT CAGAGGAAAG CCTTATTGCC
    ACAGTACAAT CCAAGTACAA GGACAGTGTT GAGTTTAAGA AACAGATTAT TGAATGTTTA
    AATAAGAGCC CCAGCGTGGA TCACTTGCTT TCCATTAAAA AGACACTGAA AAGCTTAAAG
    GCTCGACTGA GATGGAAATT GGTTGAAAAG AATAATTTGG AAGAATCTGA TGACCATGAT
    GGATCTGAGA TTGAAAAAAT AAAACAAGAA ATAACTCAGT TACGGAATAG TGTCTTTGAG
    GGAATTTATC ATGAGCAGGA GGAATATGCG AAGCTGAGTA GCTTAGTACA AAAATGGTTC
    CCTGAGCTTC CTCTACTTCA TCCTGAAATA GGATTACTTA AGTACATGAA CTCTGGTGGT
    CTTCTTACAA TGAGCTTGGA AAGAGATCTT CTTGATGCTG AGCCTATGAA GGAGCTTAGC
    AGCAAGCGTC CTTTGATATG TTCTGAGGTT AATGGGCAGA TAATTCTTTT AAAGGGCTAT
    TCTGTGGATG TCGACACAGA AGCCAGGGTA ATTCAGAGAG CAGCTGCCTA TCACACAGCT
    TGGAGAGAAG CTAAAGGAGA ATCTGGGTTG CTACCACTGA TATTCTTATT TTTGTGTAAG
    TCGGATCCTA TGGCTTATCT GATGGTCCCA TATTATCCTA GGGCAAATCT GAATGCTGTT
    CAGTCCACCA TGCCTTTAAC TTCAGAAGAA GTTTTAAAGG TCATGAAAGG TGTAGCCCAG
    GGCCTTCATA CATTGCACAA GGCTGACATA GTTCATGGAT CACTTCATCA GAACAATGTT
    TTTGCTTTAA ACCGTCAACA TGGAATTGTT GGAGATTTTG ACTTCACCAA ATCTGTGAGT
    CAGCGAACCT CAGTGAACAT GATGGTTGGT GACTTAAGTT TGCTATCACC TGAACTGAAA
    ATGGGAAAAC CTGCTTCTCC GAGTTCTGAC TTATATGCTT ATGGCTGCCT TTTATTGTGG
    CTTTCAGTTC AGAATCAGGA ATTTGAAACT AATGAGGATG GAATCCCAAA GATGGATCAG
    TTTCATTTGG ATGATAATGT CAAATCCCTC CTCTGTAGCT TGATCTGTTG TAGAAGTTCA
    ATGACTGCCG AGCAGGTTTT GAATGCTGAC TGTTTCTTGC TACCAAAGGG AAAATCAATG
    CCAAACTCAG AGAAAGATAC TGAATACATC CCCAAAAAAG AGGAAGAAGA ATTAAAGGTG
    AAGAACTTGG ATAAAGATAA GGAAAAGACA AGAAATGGTG AAGCCAGCTT TGATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005205617.1
    CDS21..3077
    Translation

    Target ORF information:

    RefSeq Version XM_005205560.3
    Organism Bos taurus(cattle)
    Definition Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005205560.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTGGGGCC TGGGGCATTC TTCTAGAACT TCTGTGGCAG GAAGTGCGAG TTTTGCAGGA 
    ATTGTCCAAA TGGAGGAAGA TAAACATTGC AATAAAGTTG AAGATGTGGT TGGAAGTCAT
    GTGGAAGATG CAGTAACATT TTGGGCCCAG AGTATCAATA GAAATAAGGA TATTATGAAG
    ATTGGTTGTT CACTGTCTGA AGTCTGTCCC CTTGCCAATT CAGTGTTTGG GAATCTTGAT
    CCAAAGAAGA TTTATGGTGG ATTATTTTCT GAAGATAAAT GTTGGTACAG ATGCCAAGTA
    CTGAAAGTCA TCAACGATGA AAAGTGTCTG GTGAGATATA TTGACTATGG AAATACAGAA
    ATTCTGAATC GATCGGATAT AGTAGAGATT CCTTTGGATC TGCAATTTTC TAGTGTTGCC
    AAAAAGTATA GACTTTGGGG ACTACAGATT CCTTCTAGCC AAGAAGTTAC CCAGTTTGAT
    CAGGGCAGAA CCTTTTTGGG GAGCTTGATT TTTGAAAAGG AAATTAAAAT GAGAATTAAA
    GCTACCTATC AAGATGGAAC AATAATTGCT CAGGCTGAGT ATGGCAATGT AGATATTGGG
    GAAGAGGTGG CTAAGAAAGG ATTTGCAGAA AAATGCAGAC TCACTTCCAG CACTAATATC
    TGTGAGGAAA AAAAATCAGA TCCTGGTCAG CTTGTCCTCA GGAACCTCAA AAGCCCCATT
    CCTTTGTGGG GGCATAGATC AAGCCAATCA AACTTCACCC GGCCAAAGGG GCGCTTAAGT
    GGGAGAATGT GTCTTGACTT GAAGAATGAA AATGATGCAG GAAATAATGT GACATTTCCA
    AAGGAAAATT TGGCTGTTGG TGACTTTAAT TTAGGGTCTA ATGTCAGCCT GGCAAAAATT
    AAGCAGGACC AGAAACTGAT TGAAGAAAAT GAAAAACTTA AAACAGAGAA GGAGGTTCTT
    CTTGAAAGGT GCAAGGCATT AGAATTGAAA GTCGAACAGA CTGCCCAGGA GCTGCAGCAA
    GAGAAAGCAA CTACCATGGA TTTGACTAAA CGTTTGGAAA GCACTGTGAA GACTTGTATA
    GGTACCAGAA TGAAAAATCT GGCAGCTAAG GTTGAAATAT TGAAAGAAAT TAGACGTGTC
    AGTATCAGTG TTCGTTTTGG AAATGACCTT TCAGATGCTA TACAAGTGTT GGATGAAGAC
    TGCTTTACTA TCCCAGCTTC TTTGAATGAA TTAGAGCTAA TTTGGGCAGA ATATAATCTG
    GCTCAGGAGA AGATTCAAAC TTGTGAGAAT GTGAATGAAG GGAATATTTT GATTGCTGAA
    AGAAATGAAG TACAACAGAA GCTGTACATG GCAGTAGAAG TTTTTATTCT GGAAGTTGAT
    GAGTTATCTC TTAATAAACG CTTAAAAACG TTGCAGGATT TGTCAGCCTC TTTAGAAGCA
    GTATATGGAC AGGCCAAAGA AGGAATTAAC TCTGAAGAAA TACTGGAGAA ATTTAATGAC
    TGGAGGTGTA ATAAAAGAGA GGAGTTCACC AGTGTTAGAA GTGAAACAGA GACTTGTATA
    CAACGTCTTA TAGCATGGTT CCAGAATACT TTAAAGGCCT TTGATCTGTC TGTGGACAAA
    TCACTGATTT CTGATGACAC AATTGATAAT ATTGATGAAA TCCTGGAGAA CACTGAGTCA
    GGTGTCTGCA AGGAGCTGGA GATATCTCTG ATTGAACAAG GTGATGCAGA CAAGGAGATC
    ATTTTAAAAA CATGTAGTCA AGTACTACAA AAGATCCGTT CAGAGGAAAG CCTTATTGCC
    ACAGTACAAT CCAAGTACAA GGACAGTGTT GAGTTTAAGA AACAGATTAT TGAATGTTTA
    AATAAGAGCC CCAGCGTGGA TCACTTGCTT TCCATTAAAA AGACACTGAA AAGCTTAAAG
    GCTCGACTGA GATGGAAATT GGTTGAAAAG AATAATTTGG AAGAATCTGA TGACCATGAT
    GGATCTGAGA TTGAAAAAAT AAAACAAGAA ATAACTCAGT TACGGAATAG TGTCTTTGAG
    GGAATTTATC ATGAGCAGGA GGAATATGCG AAGCTGAGTA GCTTAGTACA AAAATGGTTC
    CCTGAGCTTC CTCTACTTCA TCCTGAAATA GGATTACTTA AGTACATGAA CTCTGGTGGT
    CTTCTTACAA TGAGCTTGGA AAGAGATCTT CTTGATGCTG AGCCTATGAA GGAGCTTAGC
    AGCAAGCGTC CTTTGATATG TTCTGAGGTT AATGGGCAGA TAATTCTTTT AAAGGGCTAT
    TCTGTGGATG TCGACACAGA AGCCAGGGTA ATTCAGAGAG CAGCTGCCTA TCACACAGCT
    TGGAGAGAAG CTAAAGGAGA ATCTGGGTTG CTACCACTGA TATTCTTATT TTTGTGTAAG
    TCGGATCCTA TGGCTTATCT GATGGTCCCA TATTATCCTA GGGCAAATCT GAATGCTGTT
    CAGTCCACCA TGCCTTTAAC TTCAGAAGAA GTTTTAAAGG TCATGAAAGG TGTAGCCCAG
    GGCCTTCATA CATTGCACAA GGCTGACATA GTTCATGGAT CACTTCATCA GAACAATGTT
    TTTGCTTTAA ACCGTCAACA TGGAATTGTT GGAGATTTTG ACTTCACCAA ATCTGTGAGT
    CAGCGAACCT CAGTGAACAT GATGGTTGGT GACTTAAGTT TGCTATCACC TGAACTGAAA
    ATGGGAAAAC CTGCTTCTCC GAGTTCTGAC TTATATGCTT ATGGCTGCCT TTTATTGTGG
    CTTTCAGTTC AGAATCAGGA ATTTGAAACT AATGAGGATG GAATCCCAAA GATGGATCAG
    TTTCATTTGG ATGATAATGT CAAATCCCTC CTCTGTAGCT TGATCTGTTG TAGAAGTTCA
    ATGACTGCCG AGCAGGTTTT GAATGCTGAC TGTTTCTTGC TACCAAAGGG AAAATCAATG
    CCAAACTCAG AGAAAGATAC TGAATACATC CCCAAAAAAG AGGAAGAAGA ATTAAAGGTG
    AAGAACTTGG ATAAAGATAA GGAAAAGACA AGAAATGGTG AAGCCAGCTT TGATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18656
    Clone ID Related Accession (Same CDS sequence) XM_015463069.1 , XM_005205560.3 , XM_005205560.4
    Accession Version XM_005205560.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3057bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein FAM221A isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005205560.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTGGGGCC TGGGGCATTC TTCTAGAACT TCTGTGGCAG GAAGTGCGAG TTTTGCAGGA 
    ATTGTCCAAA TGGAGGAAGA TAAACATTGC AATAAAGTTG AAGATGTGGT TGGAAGTCAT
    GTGGAAGATG CAGTAACATT TTGGGCCCAG AGTATCAATA GAAATAAGGA TATTATGAAG
    ATTGGTTGTT CACTGTCTGA AGTCTGTCCC CTTGCCAATT CAGTGTTTGG GAATCTTGAT
    CCAAAGAAGA TTTATGGTGG ATTATTTTCT GAAGATAAAT GTTGGTACAG ATGCCAAGTA
    CTGAAAGTCA TCAACGATGA AAAGTGTCTG GTGAGATATA TTGACTATGG AAATACAGAA
    ATTCTGAATC GATCGGATAT AGTAGAGATT CCTTTGGATC TGCAATTTTC TAGTGTTGCC
    AAAAAGTATA GACTTTGGGG ACTACAGATT CCTTCTAGCC AAGAAGTTAC CCAGTTTGAT
    CAGGGCAGAA CCTTTTTGGG GAGCTTGATT TTTGAAAAGG AAATTAAAAT GAGAATTAAA
    GCTACCTATC AAGATGGAAC AATAATTGCT CAGGCTGAGT ATGGCAATGT AGATATTGGG
    GAAGAGGTGG CTAAGAAAGG ATTTGCAGAA AAATGCAGAC TCACTTCCAG CACTAATATC
    TGTGAGGAAA AAAAATCAGA TCCTGGTCAG CTTGTCCTCA GGAACCTCAA AAGCCCCATT
    CCTTTGTGGG GGCATAGATC AAGCCAATCA AACTTCACCC GGCCAAAGGG GCGCTTAAGT
    GGGAGAATGT GTCTTGACTT GAAGAATGAA AATGATGCAG GAAATAATGT GACATTTCCA
    AAGGAAAATT TGGCTGTTGG TGACTTTAAT TTAGGGTCTA ATGTCAGCCT GGCAAAAATT
    AAGCAGGACC AGAAACTGAT TGAAGAAAAT GAAAAACTTA AAACAGAGAA GGAGGTTCTT
    CTTGAAAGGT GCAAGGCATT AGAATTGAAA GTCGAACAGA CTGCCCAGGA GCTGCAGCAA
    GAGAAAGCAA CTACCATGGA TTTGACTAAA CGTTTGGAAA GCACTGTGAA GACTTGTATA
    GGTACCAGAA TGAAAAATCT GGCAGCTAAG GTTGAAATAT TGAAAGAAAT TAGACGTGTC
    AGTATCAGTG TTCGTTTTGG AAATGACCTT TCAGATGCTA TACAAGTGTT GGATGAAGAC
    TGCTTTACTA TCCCAGCTTC TTTGAATGAA TTAGAGCTAA TTTGGGCAGA ATATAATCTG
    GCTCAGGAGA AGATTCAAAC TTGTGAGAAT GTGAATGAAG GGAATATTTT GATTGCTGAA
    AGAAATGAAG TACAACAGAA GCTGTACATG GCAGTAGAAG TTTTTATTCT GGAAGTTGAT
    GAGTTATCTC TTAATAAACG CTTAAAAACG TTGCAGGATT TGTCAGCCTC TTTAGAAGCA
    GTATATGGAC AGGCCAAAGA AGGAATTAAC TCTGAAGAAA TACTGGAGAA ATTTAATGAC
    TGGAGGTGTA ATAAAAGAGA GGAGTTCACC AGTGTTAGAA GTGAAACAGA GACTTGTATA
    CAACGTCTTA TAGCATGGTT CCAGAATACT TTAAAGGCCT TTGATCTGTC TGTGGACAAA
    TCACTGATTT CTGATGACAC AATTGATAAT ATTGATGAAA TCCTGGAGAA CACTGAGTCA
    GGTGTCTGCA AGGAGCTGGA GATATCTCTG ATTGAACAAG GTGATGCAGA CAAGGAGATC
    ATTTTAAAAA CATGTAGTCA AGTACTACAA AAGATCCGTT CAGAGGAAAG CCTTATTGCC
    ACAGTACAAT CCAAGTACAA GGACAGTGTT GAGTTTAAGA AACAGATTAT TGAATGTTTA
    AATAAGAGCC CCAGCGTGGA TCACTTGCTT TCCATTAAAA AGACACTGAA AAGCTTAAAG
    GCTCGACTGA GATGGAAATT GGTTGAAAAG AATAATTTGG AAGAATCTGA TGACCATGAT
    GGATCTGAGA TTGAAAAAAT AAAACAAGAA ATAACTCAGT TACGGAATAG TGTCTTTGAG
    GGAATTTATC ATGAGCAGGA GGAATATGCG AAGCTGAGTA GCTTAGTACA AAAATGGTTC
    CCTGAGCTTC CTCTACTTCA TCCTGAAATA GGATTACTTA AGTACATGAA CTCTGGTGGT
    CTTCTTACAA TGAGCTTGGA AAGAGATCTT CTTGATGCTG AGCCTATGAA GGAGCTTAGC
    AGCAAGCGTC CTTTGATATG TTCTGAGGTT AATGGGCAGA TAATTCTTTT AAAGGGCTAT
    TCTGTGGATG TCGACACAGA AGCCAGGGTA ATTCAGAGAG CAGCTGCCTA TCACACAGCT
    TGGAGAGAAG CTAAAGGAGA ATCTGGGTTG CTACCACTGA TATTCTTATT TTTGTGTAAG
    TCGGATCCTA TGGCTTATCT GATGGTCCCA TATTATCCTA GGGCAAATCT GAATGCTGTT
    CAGTCCACCA TGCCTTTAAC TTCAGAAGAA GTTTTAAAGG TCATGAAAGG TGTAGCCCAG
    GGCCTTCATA CATTGCACAA GGCTGACATA GTTCATGGAT CACTTCATCA GAACAATGTT
    TTTGCTTTAA ACCGTCAACA TGGAATTGTT GGAGATTTTG ACTTCACCAA ATCTGTGAGT
    CAGCGAACCT CAGTGAACAT GATGGTTGGT GACTTAAGTT TGCTATCACC TGAACTGAAA
    ATGGGAAAAC CTGCTTCTCC GAGTTCTGAC TTATATGCTT ATGGCTGCCT TTTATTGTGG
    CTTTCAGTTC AGAATCAGGA ATTTGAAACT AATGAGGATG GAATCCCAAA GATGGATCAG
    TTTCATTTGG ATGATAATGT CAAATCCCTC CTCTGTAGCT TGATCTGTTG TAGAAGTTCA
    ATGACTGCCG AGCAGGTTTT GAATGCTGAC TGTTTCTTGC TACCAAAGGG AAAATCAATG
    CCAAACTCAG AGAAAGATAC TGAATACATC CCCAAAAAAG AGGAAGAAGA ATTAAAGGTG
    AAGAACTTGG ATAAAGATAA GGAAAAGACA AGAAATGGTG AAGCCAGCTT TGATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005205617.1
    CDS39..3095
    Translation

    Target ORF information:

    RefSeq Version XM_005205560.4
    Organism Bos taurus(cattle)
    Definition Bos taurus family with sequence similarity 221, member A (FAM221A), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005205560.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTGGGGCC TGGGGCATTC TTCTAGAACT TCTGTGGCAG GAAGTGCGAG TTTTGCAGGA 
    ATTGTCCAAA TGGAGGAAGA TAAACATTGC AATAAAGTTG AAGATGTGGT TGGAAGTCAT
    GTGGAAGATG CAGTAACATT TTGGGCCCAG AGTATCAATA GAAATAAGGA TATTATGAAG
    ATTGGTTGTT CACTGTCTGA AGTCTGTCCC CTTGCCAATT CAGTGTTTGG GAATCTTGAT
    CCAAAGAAGA TTTATGGTGG ATTATTTTCT GAAGATAAAT GTTGGTACAG ATGCCAAGTA
    CTGAAAGTCA TCAACGATGA AAAGTGTCTG GTGAGATATA TTGACTATGG AAATACAGAA
    ATTCTGAATC GATCGGATAT AGTAGAGATT CCTTTGGATC TGCAATTTTC TAGTGTTGCC
    AAAAAGTATA GACTTTGGGG ACTACAGATT CCTTCTAGCC AAGAAGTTAC CCAGTTTGAT
    CAGGGCAGAA CCTTTTTGGG GAGCTTGATT TTTGAAAAGG AAATTAAAAT GAGAATTAAA
    GCTACCTATC AAGATGGAAC AATAATTGCT CAGGCTGAGT ATGGCAATGT AGATATTGGG
    GAAGAGGTGG CTAAGAAAGG ATTTGCAGAA AAATGCAGAC TCACTTCCAG CACTAATATC
    TGTGAGGAAA AAAAATCAGA TCCTGGTCAG CTTGTCCTCA GGAACCTCAA AAGCCCCATT
    CCTTTGTGGG GGCATAGATC AAGCCAATCA AACTTCACCC GGCCAAAGGG GCGCTTAAGT
    GGGAGAATGT GTCTTGACTT GAAGAATGAA AATGATGCAG GAAATAATGT GACATTTCCA
    AAGGAAAATT TGGCTGTTGG TGACTTTAAT TTAGGGTCTA ATGTCAGCCT GGCAAAAATT
    AAGCAGGACC AGAAACTGAT TGAAGAAAAT GAAAAACTTA AAACAGAGAA GGAGGTTCTT
    CTTGAAAGGT GCAAGGCATT AGAATTGAAA GTCGAACAGA CTGCCCAGGA GCTGCAGCAA
    GAGAAAGCAA CTACCATGGA TTTGACTAAA CGTTTGGAAA GCACTGTGAA GACTTGTATA
    GGTACCAGAA TGAAAAATCT GGCAGCTAAG GTTGAAATAT TGAAAGAAAT TAGACGTGTC
    AGTATCAGTG TTCGTTTTGG AAATGACCTT TCAGATGCTA TACAAGTGTT GGATGAAGAC
    TGCTTTACTA TCCCAGCTTC TTTGAATGAA TTAGAGCTAA TTTGGGCAGA ATATAATCTG
    GCTCAGGAGA AGATTCAAAC TTGTGAGAAT GTGAATGAAG GGAATATTTT GATTGCTGAA
    AGAAATGAAG TACAACAGAA GCTGTACATG GCAGTAGAAG TTTTTATTCT GGAAGTTGAT
    GAGTTATCTC TTAATAAACG CTTAAAAACG TTGCAGGATT TGTCAGCCTC TTTAGAAGCA
    GTATATGGAC AGGCCAAAGA AGGAATTAAC TCTGAAGAAA TACTGGAGAA ATTTAATGAC
    TGGAGGTGTA ATAAAAGAGA GGAGTTCACC AGTGTTAGAA GTGAAACAGA GACTTGTATA
    CAACGTCTTA TAGCATGGTT CCAGAATACT TTAAAGGCCT TTGATCTGTC TGTGGACAAA
    TCACTGATTT CTGATGACAC AATTGATAAT ATTGATGAAA TCCTGGAGAA CACTGAGTCA
    GGTGTCTGCA AGGAGCTGGA GATATCTCTG ATTGAACAAG GTGATGCAGA CAAGGAGATC
    ATTTTAAAAA CATGTAGTCA AGTACTACAA AAGATCCGTT CAGAGGAAAG CCTTATTGCC
    ACAGTACAAT CCAAGTACAA GGACAGTGTT GAGTTTAAGA AACAGATTAT TGAATGTTTA
    AATAAGAGCC CCAGCGTGGA TCACTTGCTT TCCATTAAAA AGACACTGAA AAGCTTAAAG
    GCTCGACTGA GATGGAAATT GGTTGAAAAG AATAATTTGG AAGAATCTGA TGACCATGAT
    GGATCTGAGA TTGAAAAAAT AAAACAAGAA ATAACTCAGT TACGGAATAG TGTCTTTGAG
    GGAATTTATC ATGAGCAGGA GGAATATGCG AAGCTGAGTA GCTTAGTACA AAAATGGTTC
    CCTGAGCTTC CTCTACTTCA TCCTGAAATA GGATTACTTA AGTACATGAA CTCTGGTGGT
    CTTCTTACAA TGAGCTTGGA AAGAGATCTT CTTGATGCTG AGCCTATGAA GGAGCTTAGC
    AGCAAGCGTC CTTTGATATG TTCTGAGGTT AATGGGCAGA TAATTCTTTT AAAGGGCTAT
    TCTGTGGATG TCGACACAGA AGCCAGGGTA ATTCAGAGAG CAGCTGCCTA TCACACAGCT
    TGGAGAGAAG CTAAAGGAGA ATCTGGGTTG CTACCACTGA TATTCTTATT TTTGTGTAAG
    TCGGATCCTA TGGCTTATCT GATGGTCCCA TATTATCCTA GGGCAAATCT GAATGCTGTT
    CAGTCCACCA TGCCTTTAAC TTCAGAAGAA GTTTTAAAGG TCATGAAAGG TGTAGCCCAG
    GGCCTTCATA CATTGCACAA GGCTGACATA GTTCATGGAT CACTTCATCA GAACAATGTT
    TTTGCTTTAA ACCGTCAACA TGGAATTGTT GGAGATTTTG ACTTCACCAA ATCTGTGAGT
    CAGCGAACCT CAGTGAACAT GATGGTTGGT GACTTAAGTT TGCTATCACC TGAACTGAAA
    ATGGGAAAAC CTGCTTCTCC GAGTTCTGAC TTATATGCTT ATGGCTGCCT TTTATTGTGG
    CTTTCAGTTC AGAATCAGGA ATTTGAAACT AATGAGGATG GAATCCCAAA GATGGATCAG
    TTTCATTTGG ATGATAATGT CAAATCCCTC CTCTGTAGCT TGATCTGTTG TAGAAGTTCA
    ATGACTGCCG AGCAGGTTTT GAATGCTGAC TGTTTCTTGC TACCAAAGGG AAAATCAATG
    CCAAACTCAG AGAAAGATAC TGAATACATC CCCAAAAAAG AGGAAGAAGA ATTAAAGGTG
    AAGAACTTGG ATAAAGATAA GGAAAAGACA AGAAATGGTG AAGCCAGCTT TGATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.