MTHFR cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	MTHFR
Entrez Gene ID	497032
Full Name	methylenetetrahydrofolate reductase
General protein information	Preferred Names methylenetetrahydrofolate reductase Names methylenetetrahydrofolate reductase 5,10-methylenetetrahydrofolate reductase (NADPH) methylenetetrahydrofolate reductase (NAD(P)H)
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015475084.1	XP_015330570.1	methylenetetrahydrofolate reductase isoform X2
XM_005216932.4	XP_005216989.1	methylenetetrahydrofolate reductase isoform X1
NM_001011685.1	NP_001011685.1	methylenetetrahydrofolate reductase
XM_015475084.2	XP_015330570.1	methylenetetrahydrofolate reductase isoform X2
XM_005216932.3	XP_005216989.1	methylenetetrahydrofolate reductase isoform X1
XM_024976080.1	XP_024831848.1	methylenetetrahydrofolate reductase isoform X3

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Mthfr	XP_342976.3
Danio rerio (zebrafish)	mthfr	NP_001121727.1
Kluyveromyces lactis	KLLA0F09647g	XP_455518.1
Magnaporthe oryzae (rice blast fungus)	MGG_01728	XP_003714725.1
Oryza sativa (rice)	Os03g0815200	NP_001051683.1
Mus musculus (house mouse)	Mthfr	NP_001155270.1
Bos taurus (cattle)	MTHFR	NP_001011685.1
Gallus gallus (chicken)	MTHFR	XP_417645.3
Caenorhabditis elegans (roundworm)	mthf-1	NP_741028.1
Arabidopsis thaliana (thale cress)	MTHFR2	NP_566011.1
Xenopus tropicalis (tropical clawed frog)	mthfr	NP_001096464.1
Homo sapiens (human)	MTHFR	NP_005948.3
Pan troglodytes (chimpanzee)	MTHFR	XP_001141040.1
	LOC714840	XP_001105017.1
Canis lupus familiaris (dog)	MTHFR	XP_005618049.1
Saccharomyces cerevisiae (baker's yeast)	MET13	NP_011390.2
Eremothecium gossypii	AGOS_AAR170W	NP_982713.2
Schizosaccharomyces pombe (fission yeast)	met9	NP_593224.1
Neurospora crassa	NCU07690	XP_961729.1
Arabidopsis thaliana (thale cress)	MTHFR1	NP_191556.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MTHFR gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MTHFR cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa42088	NM_001011685.1 Latest version!	Bos taurus methylenetetrahydrofolate reductase (MTHFR), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa27181	XM_005216932.4 Latest version!	Bos taurus methylenetetrahydrofolate reductase (MTHFR), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa223464	XM_024976080.1 Latest version!	Bos taurus methylenetetrahydrofolate reductase (MTHFR), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OBa92927	XM_015475084.2 Latest version!	Bos taurus methylenetetrahydrofolate reductase (MTHFR), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
View Old Accession Versions >>
OBa92927	XM_015475084.1	Bos taurus methylenetetrahydrofolate reductase (NAD(P)H) (MTHFR), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OBa27181	XM_005216932.3	Bos taurus methylenetetrahydrofolate reductase (NAD(P)H) (MTHFR), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MTHFR ( NM_001011685.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001011685.1 Bos taurus methylenetetrahydrofolate reductase (MTHFR), mRNA.

Price & Availability↑

CloneID	OBa42088
Clone ID Related Accession (Same CDS sequence)	NM_001011685.1
Accession Version	NM_001011685.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Bos taurus(cattle)
Product	methylenetetrahydrofolate reductase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY854631.1. On Mar 29, 2005 this sequence version replaced XM_581323.1. ##Evidence-Data-START## Transcript exon combination :: AY854631.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145414 [ECO:0000348] ##Evidence-Data-END##

ATGGTGAACG AACCCAGAGG GAACGGCAGC CCCGGCCCCC GCTGGGAGGG CAGCAGCAGC 
GGCAGCGAGA GCTCAAGGAC AAGTTCAAGG TGCTCCACGC CGGGGTTGGA CCCCGAGCGA 
TGCGAGAGAC TCCGGGAGAA GATGAAGCGG AAGATGGACT CTGGAGACAA GTGGTTCTCC 
CTAGAGTTCT TCCCTCCCCG AACTGCCCAG GGTGCTGTCA ATCTCATCTC CAGGTTTGAC 
CGGATGGGAG CGGGTGGCCC CCTCTTTGTA GACGTGACCT GGCACCCAGC CGGGGATCCT 
GGCTCCGACA AGGAGACCTC GTCCATGGTG ATTGCCAGCA CTGCCGTGAA CTACTGCGGC 
CTCGAGACAA TCCTGCACAT GACCTGCTGC CATCAGAGCC GTGAAGAGAT CACGGGCCAC 
CTGAACAAAG CCAAGCAGCT GGGCCTGAAG AACATCCTGG CGCTGAGGGG AGACCCCATA 
GGTGACCAAT GGGAAGAGGA GGAAGGAGGC TTCAACTATG CTACGGACTT GGTGAAGCAC 
ATCCGAAACG AGTTTGGTGA CTACTTTGAC GTCTGTGTGG CAGGTTACCC CAAAGGCCAC 
CCTGAAGGAG AGAGCTTTGA GGCTGATCTG AAGCACCTGA AGGAGAAGGT GGCTGCAGGA 
GCCGACTTCA TCATCACCCA GCTATTCTTT GAGGCTGAAA CATTCTTCCG CTTTGTGAAG 
GCTTGCTCCG AGATAGGCAT TACCTGCCCC GTCCTCCCCG GCATCTTCCC AATTCAGGGC 
TACCACTCCC TCCGGCAGCT GGTGAAACTG TCCAAACTGG AGGTGCCACA GCAGATCAAG 
GATGTGATCG AGCCCATCAA AGACAATGAT GCTGCCATCC GCAACTATGG CATCGAGCAG 
GCCGTGAGCC TGTGCCAGGA GCTGCTGGCC AGTGGCTTGG TTCCAGGCCT CCACTTCTAC 
ACCCTCAACC GCGAGGTGGC CACCATCGAG GTGCTGAAGC GCCTGGGGCT TTGGATTGAG 
GACCCTAGGC GTCCCCTGCC CTGGGCCCTC AGTGCCCACC CCAAGCGCCG GGTGGAGGAT 
GTCCGGCCCA TCTTCTGGGC CTCGAGACCA AAGAGTTACA TTTACCGTAC GCAGGAGTGG 
GACGAGTTCC CCAATGGCCG CTGGGGCAAT TCCTCCTCTC CGGCCTTTGG GGAGCTGAAG 
GACTACTACC TCTTCTACCT AAAGAGCAAG TCCCCGAAGG AGGAGCTGCT CAAGATGTGG 
GGGGAGGAGC TGACCAGTGA GGAAAGCGTC TTCCAAGTCT TTGCCCACCA CCTCTCGGGA 
GAGCCCAACC AGAATGGCTA CAAAGTGACT TGCCTGCCCT GGAATGATGA GCCCCTGGCA 
GCCGAGACCA GCCTGATGAA GGAGGAGCTG CTGAGAGTGA ACCGGCGAGG CATCCTCACC 
ATCAACTCCC AGCCCAACAT CAACGGGAAG CCGTCCTCTG ACCCCATCGT GGGCTGGGGC 
CCCAGTGGGG GCTATGTCTT CCAGAAGGCC TACTTAGAGT TCTTCACTTC CCGAGAAACG 
GTGGAGGCGC TTTTGCAGGT GCTGAAGAAG TATGAACTCC GGGTGAATTA CCACATCGTG 
GATGTGAAGG GTGAAAATAT CACCAACGCC CCTGAGCTGC AACCCAACGC GGTCACCTGG 
GGCATCTTCC CTGGCCGAGA GATCATCCAG CCCACAGTGG TGGACCCGGT CAGCTTCATG 
TTCTGGAAGG ATGAGGCCTT CGCGCTGTGG ATTGAGCAGT GGGGCAAGCT GTATGAGGAG 
GAGTCCCCCT CCCGCATGAT CATCCAGTAC ATCCACGACA ACTACTTCCT GGTCAACCTG 
GTGGACAACG AGTTTCCGCT GGACAACTGC CTGTGGCAGG TGGTGGAAGA CACGTTTGAG 
CTTCTCAGCA GGCCCCCCCA AGACAAGAGG GAGACAGAGG CGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001011685.1
CDS	192..2159
Translation	MVNEPRGNGSPGPRWEGSSSGSESSRTSSRCSTPGLDPERCERLREKMKRKMDSGDKWFSLEFFPPRTAQGAVNLISRFDRMGAGGPLFVDVTWHPAGDPGSDKETSSMVIASTAVNYCGLETILHMTCCHQSREEITGHLNKAKQLGLKNILALRGDPIGDQWEEEEGGFNYATDLVKHIRNEFGDYFDVCVAGYPKGHPEGESFEADLKHLKEKVAAGADFIITQLFFEAETFFRFVKACSEIGITCPVLPGIFPIQGYHSLRQLVKLSKLEVPQQIKDVIEPIKDNDAAIRNYGIEQAVSLCQELLASGLVPGLHFYTLNREVATIEVLKRLGLWIEDPRRPLPWALSAHPKRRVEDVRPIFWASRPKSYIYRTQEWDEFPNGRWGNSSSPAFGELKDYYLFYLKSKSPKEELLKMWGEELTSEESVFQVFAHHLSGEPNQNGYKVTCLPWNDEPLAAETSLMKEELLRVNRRGILTINSQPNINGKPSSDPIVGWGPSGGYVFQKAYLEFFTSRETVEALLQVLKKYELRVNYHIVDVKGENITNAPELQPNAVTWGIFPGREIIQPTVVDPVSFMFWKDEAFALWIEQWGKLYEEESPSRMIIQYIHDNYFLVNLVDNEFPLDNCLWQVVEDTFELLSRPPQDKRETEAL

Target ORF information:

RefSeq Version	NM_001011685.1
Organism	Bos taurus(cattle)
Definition	Bos taurus methylenetetrahydrofolate reductase (MTHFR), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001011685.1

ORF Insert Sequence:

ATGGTGAACG AACCCAGAGG GAACGGCAGC CCCGGCCCCC GCTGGGAGGG CAGCAGCAGC 
GGCAGCGAGA GCTCAAGGAC AAGTTCAAGG TGCTCCACGC CGGGGTTGGA CCCCGAGCGA 
TGCGAGAGAC TCCGGGAGAA GATGAAGCGG AAGATGGACT CTGGAGACAA GTGGTTCTCC 
CTAGAGTTCT TCCCTCCCCG AACTGCCCAG GGTGCTGTCA ATCTCATCTC CAGGTTTGAC 
CGGATGGGAG CGGGTGGCCC CCTCTTTGTA GACGTGACCT GGCACCCAGC CGGGGATCCT 
GGCTCCGACA AGGAGACCTC GTCCATGGTG ATTGCCAGCA CTGCCGTGAA CTACTGCGGC 
CTCGAGACAA TCCTGCACAT GACCTGCTGC CATCAGAGCC GTGAAGAGAT CACGGGCCAC 
CTGAACAAAG CCAAGCAGCT GGGCCTGAAG AACATCCTGG CGCTGAGGGG AGACCCCATA 
GGTGACCAAT GGGAAGAGGA GGAAGGAGGC TTCAACTATG CTACGGACTT GGTGAAGCAC 
ATCCGAAACG AGTTTGGTGA CTACTTTGAC GTCTGTGTGG CAGGTTACCC CAAAGGCCAC 
CCTGAAGGAG AGAGCTTTGA GGCTGATCTG AAGCACCTGA AGGAGAAGGT GGCTGCAGGA 
GCCGACTTCA TCATCACCCA GCTATTCTTT GAGGCTGAAA CATTCTTCCG CTTTGTGAAG 
GCTTGCTCCG AGATAGGCAT TACCTGCCCC GTCCTCCCCG GCATCTTCCC AATTCAGGGC 
TACCACTCCC TCCGGCAGCT GGTGAAACTG TCCAAACTGG AGGTGCCACA GCAGATCAAG 
GATGTGATCG AGCCCATCAA AGACAATGAT GCTGCCATCC GCAACTATGG CATCGAGCAG 
GCCGTGAGCC TGTGCCAGGA GCTGCTGGCC AGTGGCTTGG TTCCAGGCCT CCACTTCTAC 
ACCCTCAACC GCGAGGTGGC CACCATCGAG GTGCTGAAGC GCCTGGGGCT TTGGATTGAG 
GACCCTAGGC GTCCCCTGCC CTGGGCCCTC AGTGCCCACC CCAAGCGCCG GGTGGAGGAT 
GTCCGGCCCA TCTTCTGGGC CTCGAGACCA AAGAGTTACA TTTACCGTAC GCAGGAGTGG 
GACGAGTTCC CCAATGGCCG CTGGGGCAAT TCCTCCTCTC CGGCCTTTGG GGAGCTGAAG 
GACTACTACC TCTTCTACCT AAAGAGCAAG TCCCCGAAGG AGGAGCTGCT CAAGATGTGG 
GGGGAGGAGC TGACCAGTGA GGAAAGCGTC TTCCAAGTCT TTGCCCACCA CCTCTCGGGA 
GAGCCCAACC AGAATGGCTA CAAAGTGACT TGCCTGCCCT GGAATGATGA GCCCCTGGCA 
GCCGAGACCA GCCTGATGAA GGAGGAGCTG CTGAGAGTGA ACCGGCGAGG CATCCTCACC 
ATCAACTCCC AGCCCAACAT CAACGGGAAG CCGTCCTCTG ACCCCATCGT GGGCTGGGGC 
CCCAGTGGGG GCTATGTCTT CCAGAAGGCC TACTTAGAGT TCTTCACTTC CCGAGAAACG 
GTGGAGGCGC TTTTGCAGGT GCTGAAGAAG TATGAACTCC GGGTGAATTA CCACATCGTG 
GATGTGAAGG GTGAAAATAT CACCAACGCC CCTGAGCTGC AACCCAACGC GGTCACCTGG 
GGCATCTTCC CTGGCCGAGA GATCATCCAG CCCACAGTGG TGGACCCGGT CAGCTTCATG 
TTCTGGAAGG ATGAGGCCTT CGCGCTGTGG ATTGAGCAGT GGGGCAAGCT GTATGAGGAG 
GAGTCCCCCT CCCGCATGAT CATCCAGTAC ATCCACGACA ACTACTTCCT GGTCAACCTG 
GTGGACAACG AGTTTCCGCT GGACAACTGC CTGTGGCAGG TGGTGGAAGA CACGTTTGAG 
CTTCTCAGCA GGCCCCCCCA AGACAAGAGG GAGACAGAGG CGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTHFR ( XM_005216932.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005216989.1 Bos taurus methylenetetrahydrofolate reductase (MTHFR), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa27181
Clone ID Related Accession (Same CDS sequence)	XM_005216932.4 , XM_005216932.3
Accession Version	XM_005216932.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2091bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	methylenetetrahydrofolate reductase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005216932.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTGCC CAAAACCCAA GGTCTTCCCA GTGGGGCACT GCTGCCCCTC CCTGGGAATG 
CGAGCTTCGG AGGCTGGCAG CGGAAGGCTC TCAGTGCCAC CCTCCATCAG CAGGAACTCG 
GCCATGGTGA ACGAACCCAG AGGGAACGGC AGCCCCGGCC CCCGCTGGGA GGGCAGCAGC 
AGCGGCAGCG AGAGCTCCAA GGACAGTTCA AGGTGCTCCA CGCCGGGGTT GGACCCCGAG 
CGATGCGAGA GACTCCGGGA GAAGATGAAG CGGAGGATGG ACTCTGGAGA CAAGTGGTTC 
TCCCTAGAGT TCTTCCCTCC CCGAACTGCC CAGGGTGCTG TCAATCTCAT CTCCAGGTTT 
GACCGGATGG GAGCGGGTGG CCCCCTCTTT GTAGACGTGA CCTGGCACCC AGCCGGGGAT 
CCTGGCTCCG ACAAGGAGAC CTCGTCCATG GTGATTGCCA GCACTGCCGT GAACTACTGC 
GGCCTCGAGA CAATCCTGCA CATGACCTGC TGCCATCAGA GCCGTGAAGA GATCACGGGC 
CACCTGAACA AAGCCAAGCA GCTGGGCCTG AAGAACATCC TGGCGCTGAG GGGAGACCCC 
ATAGGTGACC AATGGGAAGA GGAGGAAGGA GGCTTCAACT ATGCTACGGA CTTGGTGAAG 
CACATCCGAA ACGAGTTTGG TGACTACTTT GACGTCTGTG TGGCAGGTTA CCCCAAAGGC 
CACCCTGAAG GAGAGAGCTT TGAGGCTGAT CTGAAGCACC TGAAGGAGAA GGTGGCTGCA 
GGAGCCGACT TCATCATCAC CCAGCTATTC TTTGAGGCTG AAACATTCTT CCGCTTTGTG 
AAGGCTTGCT CCGAGATAGG CATTACCTGC CCCATCCTCC CCGGCATCTT CCCAATTCAG 
GGCTACCACT CCCTCCGGCA GCTGGTGAAA CTATCCAAAC TGGAGGTGCC ACAGCAGATC 
AAGGATGTGA TCGAGCCCAT CAAAGACAAT GATGCTGCCA TCCGCAACTA TGGCATCGAG 
CAGGCCGTGA GCCTGTGCCA GGAGCTGCTG GCCAGTGGCT TGGTTCCAGG CCTCCACTTC 
TACACCCTCA ACCGCGAGGT GGCCACCATC GAGGTGCTGA AGCGCCTGGG GCTTTGGATT 
GAGGACCCTA GGCGTCCCCT GCCCTGGGCC CTCAGTGCCC ACCCCAAGCG CCGGGTGGAG 
GATGTCCGGC CCATCTTCTG GGCCTCGAGA CCAAAGAGTT ACATTTACCG TACGCAGGAG 
TGGGACGAGT TCCCCAATGG CCGCTGGGGC AATTCCTCCT CTCCGGCCTT TGGGGAGCTG 
AAGGACTACT ACCTCTTCTA CCTAAAGAGC AAGTCCCCGA AGGAAGAGCT GCTCAAGATG 
TGGGGGGAGG AGCTGACCAG TGAGGAAAGC GTCTTTCAAG TCTTTGCCCA CTACCTCTCG 
GGAGAGCCCA ACCAGAATGG CTACAAAGTG ACTTGCCTGC CCTGGAATGA TGAGCCCCTG 
GCAGCCGAGA CCAGCCTGAT GAAGGAGGAG CTGCTGAGAG TGAACCGGCG GGGCATCCTC 
ACCATCAACT CCCAGCCCAA CATCAACGGG AAGCCGTCCT CTGACCCCAT TGTGGGCTGG 
GGCCCCAGTG GGGGCTATGT CTTCCAGAAG GCCTACTTAG AGTTCTTCAC TTCCCGAGAA 
ACGGTGGAGG CGCTTTTGCA GGTGCTGAAG AAGTATGAAC TCCGGGTGAA TTACCACATC 
GTGGATGTGA AGGGTGAAAA TATCACCAAC GCCCCTGAGC TGCAACCCAA CGCGGTCACC 
TGGGGCATCT TCCCTGGCCG AGAGATCATC CAGCCCACAG TGGTGGACCC GGTCAGCTTC 
ATGTTCTGGA AGGATGAGGC CTTCGCGCTG TGGATTGAGC AGTGGGGCAA GCTGTATGAG 
GAGGAGTCCC CCTCCCGCAT GATCATCCAG TACATCCACG ACAACTACTT CCTGGTCAAC 
CTGGTGGACA ACGAGTTTCC GCTGGACAAC TGCCTGTGGC AGGTGGTGGA AGACACGTTT 
GAGCTTCTCA GCAGGCCCCC CCAAGACAAG AGGGAGACAG AGGCGCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005216989.1
CDS	99..2189
Translation	MDCPKPKVFPVGHCCPSLGMRASEAGSGRLSVPPSISRNSAMVNEPRGNGSPGPRWEGSSSGSESSKDSSRCSTPGLDPERCERLREKMKRRMDSGDKWFSLEFFPPRTAQGAVNLISRFDRMGAGGPLFVDVTWHPAGDPGSDKETSSMVIASTAVNYCGLETILHMTCCHQSREEITGHLNKAKQLGLKNILALRGDPIGDQWEEEEGGFNYATDLVKHIRNEFGDYFDVCVAGYPKGHPEGESFEADLKHLKEKVAAGADFIITQLFFEAETFFRFVKACSEIGITCPILPGIFPIQGYHSLRQLVKLSKLEVPQQIKDVIEPIKDNDAAIRNYGIEQAVSLCQELLASGLVPGLHFYTLNREVATIEVLKRLGLWIEDPRRPLPWALSAHPKRRVEDVRPIFWASRPKSYIYRTQEWDEFPNGRWGNSSSPAFGELKDYYLFYLKSKSPKEELLKMWGEELTSEESVFQVFAHYLSGEPNQNGYKVTCLPWNDEPLAAETSLMKEELLRVNRRGILTINSQPNINGKPSSDPIVGWGPSGGYVFQKAYLEFFTSRETVEALLQVLKKYELRVNYHIVDVKGENITNAPELQPNAVTWGIFPGREIIQPTVVDPVSFMFWKDEAFALWIEQWGKLYEEESPSRMIIQYIHDNYFLVNLVDNEFPLDNCLWQVVEDTFELLSRPPQDKRETEAL

Target ORF information:

RefSeq Version	XM_005216932.4
Organism	Bos taurus(cattle)
Definition	Bos taurus methylenetetrahydrofolate reductase (MTHFR), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005216932.4

ORF Insert Sequence:

ATGGACTGCC CAAAACCCAA GGTCTTCCCA GTGGGGCACT GCTGCCCCTC CCTGGGAATG 
CGAGCTTCGG AGGCTGGCAG CGGAAGGCTC TCAGTGCCAC CCTCCATCAG CAGGAACTCG 
GCCATGGTGA ACGAACCCAG AGGGAACGGC AGCCCCGGCC CCCGCTGGGA GGGCAGCAGC 
AGCGGCAGCG AGAGCTCCAA GGACAGTTCA AGGTGCTCCA CGCCGGGGTT GGACCCCGAG 
CGATGCGAGA GACTCCGGGA GAAGATGAAG CGGAGGATGG ACTCTGGAGA CAAGTGGTTC 
TCCCTAGAGT TCTTCCCTCC CCGAACTGCC CAGGGTGCTG TCAATCTCAT CTCCAGGTTT 
GACCGGATGG GAGCGGGTGG CCCCCTCTTT GTAGACGTGA CCTGGCACCC AGCCGGGGAT 
CCTGGCTCCG ACAAGGAGAC CTCGTCCATG GTGATTGCCA GCACTGCCGT GAACTACTGC 
GGCCTCGAGA CAATCCTGCA CATGACCTGC TGCCATCAGA GCCGTGAAGA GATCACGGGC 
CACCTGAACA AAGCCAAGCA GCTGGGCCTG AAGAACATCC TGGCGCTGAG GGGAGACCCC 
ATAGGTGACC AATGGGAAGA GGAGGAAGGA GGCTTCAACT ATGCTACGGA CTTGGTGAAG 
CACATCCGAA ACGAGTTTGG TGACTACTTT GACGTCTGTG TGGCAGGTTA CCCCAAAGGC 
CACCCTGAAG GAGAGAGCTT TGAGGCTGAT CTGAAGCACC TGAAGGAGAA GGTGGCTGCA 
GGAGCCGACT TCATCATCAC CCAGCTATTC TTTGAGGCTG AAACATTCTT CCGCTTTGTG 
AAGGCTTGCT CCGAGATAGG CATTACCTGC CCCATCCTCC CCGGCATCTT CCCAATTCAG 
GGCTACCACT CCCTCCGGCA GCTGGTGAAA CTATCCAAAC TGGAGGTGCC ACAGCAGATC 
AAGGATGTGA TCGAGCCCAT CAAAGACAAT GATGCTGCCA TCCGCAACTA TGGCATCGAG 
CAGGCCGTGA GCCTGTGCCA GGAGCTGCTG GCCAGTGGCT TGGTTCCAGG CCTCCACTTC 
TACACCCTCA ACCGCGAGGT GGCCACCATC GAGGTGCTGA AGCGCCTGGG GCTTTGGATT 
GAGGACCCTA GGCGTCCCCT GCCCTGGGCC CTCAGTGCCC ACCCCAAGCG CCGGGTGGAG 
GATGTCCGGC CCATCTTCTG GGCCTCGAGA CCAAAGAGTT ACATTTACCG TACGCAGGAG 
TGGGACGAGT TCCCCAATGG CCGCTGGGGC AATTCCTCCT CTCCGGCCTT TGGGGAGCTG 
AAGGACTACT ACCTCTTCTA CCTAAAGAGC AAGTCCCCGA AGGAAGAGCT GCTCAAGATG 
TGGGGGGAGG AGCTGACCAG TGAGGAAAGC GTCTTTCAAG TCTTTGCCCA CTACCTCTCG 
GGAGAGCCCA ACCAGAATGG CTACAAAGTG ACTTGCCTGC CCTGGAATGA TGAGCCCCTG 
GCAGCCGAGA CCAGCCTGAT GAAGGAGGAG CTGCTGAGAG TGAACCGGCG GGGCATCCTC 
ACCATCAACT CCCAGCCCAA CATCAACGGG AAGCCGTCCT CTGACCCCAT TGTGGGCTGG 
GGCCCCAGTG GGGGCTATGT CTTCCAGAAG GCCTACTTAG AGTTCTTCAC TTCCCGAGAA 
ACGGTGGAGG CGCTTTTGCA GGTGCTGAAG AAGTATGAAC TCCGGGTGAA TTACCACATC 
GTGGATGTGA AGGGTGAAAA TATCACCAAC GCCCCTGAGC TGCAACCCAA CGCGGTCACC 
TGGGGCATCT TCCCTGGCCG AGAGATCATC CAGCCCACAG TGGTGGACCC GGTCAGCTTC 
ATGTTCTGGA AGGATGAGGC CTTCGCGCTG TGGATTGAGC AGTGGGGCAA GCTGTATGAG 
GAGGAGTCCC CCTCCCGCAT GATCATCCAG TACATCCACG ACAACTACTT CCTGGTCAAC 
CTGGTGGACA ACGAGTTTCC GCTGGACAAC TGCCTGTGGC AGGTGGTGGA AGACACGTTT 
GAGCTTCTCA GCAGGCCCCC CCAAGACAAG AGGGAGACAG AGGCGCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTHFR ( XM_005216932.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005216989.1 Bos taurus methylenetetrahydrofolate reductase (NAD(P)H) (MTHFR), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa27181
Clone ID Related Accession (Same CDS sequence)	XM_005216932.4 , XM_005216932.3
Accession Version	XM_005216932.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2091bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	methylenetetrahydrofolate reductase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104438.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005216932.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTGCC CAAAACCCAA GGTCTTCCCA GTGGGGCACT GCTGCCCCTC CCTGGGAATG 
CGAGCTTCGG AGGCTGGCAG CGGAAGGCTC TCAGTGCCAC CCTCCATCAG CAGGAACTCG 
GCCATGGTGA ACGAACCCAG AGGGAACGGC AGCCCCGGCC CCCGCTGGGA GGGCAGCAGC 
AGCGGCAGCG AGAGCTCCAA GGACAGTTCA AGGTGCTCCA CGCCGGGGTT GGACCCCGAG 
CGATGCGAGA GACTCCGGGA GAAGATGAAG CGGAGGATGG ACTCTGGAGA CAAGTGGTTC 
TCCCTAGAGT TCTTCCCTCC CCGAACTGCC CAGGGTGCTG TCAATCTCAT CTCCAGGTTT 
GACCGGATGG GAGCGGGTGG CCCCCTCTTT GTAGACGTGA CCTGGCACCC AGCCGGGGAT 
CCTGGCTCCG ACAAGGAGAC CTCGTCCATG GTGATTGCCA GCACTGCCGT GAACTACTGC 
GGCCTCGAGA CAATCCTGCA CATGACCTGC TGCCATCAGA GCCGTGAAGA GATCACGGGC 
CACCTGAACA AAGCCAAGCA GCTGGGCCTG AAGAACATCC TGGCGCTGAG GGGAGACCCC 
ATAGGTGACC AATGGGAAGA GGAGGAAGGA GGCTTCAACT ATGCTACGGA CTTGGTGAAG 
CACATCCGAA ACGAGTTTGG TGACTACTTT GACGTCTGTG TGGCAGGTTA CCCCAAAGGC 
CACCCTGAAG GAGAGAGCTT TGAGGCTGAT CTGAAGCACC TGAAGGAGAA GGTGGCTGCA 
GGAGCCGACT TCATCATCAC CCAGCTATTC TTTGAGGCTG AAACATTCTT CCGCTTTGTG 
AAGGCTTGCT CCGAGATAGG CATTACCTGC CCCATCCTCC CCGGCATCTT CCCAATTCAG 
GGCTACCACT CCCTCCGGCA GCTGGTGAAA CTATCCAAAC TGGAGGTGCC ACAGCAGATC 
AAGGATGTGA TCGAGCCCAT CAAAGACAAT GATGCTGCCA TCCGCAACTA TGGCATCGAG 
CAGGCCGTGA GCCTGTGCCA GGAGCTGCTG GCCAGTGGCT TGGTTCCAGG CCTCCACTTC 
TACACCCTCA ACCGCGAGGT GGCCACCATC GAGGTGCTGA AGCGCCTGGG GCTTTGGATT 
GAGGACCCTA GGCGTCCCCT GCCCTGGGCC CTCAGTGCCC ACCCCAAGCG CCGGGTGGAG 
GATGTCCGGC CCATCTTCTG GGCCTCGAGA CCAAAGAGTT ACATTTACCG TACGCAGGAG 
TGGGACGAGT TCCCCAATGG CCGCTGGGGC AATTCCTCCT CTCCGGCCTT TGGGGAGCTG 
AAGGACTACT ACCTCTTCTA CCTAAAGAGC AAGTCCCCGA AGGAAGAGCT GCTCAAGATG 
TGGGGGGAGG AGCTGACCAG TGAGGAAAGC GTCTTTCAAG TCTTTGCCCA CTACCTCTCG 
GGAGAGCCCA ACCAGAATGG CTACAAAGTG ACTTGCCTGC CCTGGAATGA TGAGCCCCTG 
GCAGCCGAGA CCAGCCTGAT GAAGGAGGAG CTGCTGAGAG TGAACCGGCG GGGCATCCTC 
ACCATCAACT CCCAGCCCAA CATCAACGGG AAGCCGTCCT CTGACCCCAT TGTGGGCTGG 
GGCCCCAGTG GGGGCTATGT CTTCCAGAAG GCCTACTTAG AGTTCTTCAC TTCCCGAGAA 
ACGGTGGAGG CGCTTTTGCA GGTGCTGAAG AAGTATGAAC TCCGGGTGAA TTACCACATC 
GTGGATGTGA AGGGTGAAAA TATCACCAAC GCCCCTGAGC TGCAACCCAA CGCGGTCACC 
TGGGGCATCT TCCCTGGCCG AGAGATCATC CAGCCCACAG TGGTGGACCC GGTCAGCTTC 
ATGTTCTGGA AGGATGAGGC CTTCGCGCTG TGGATTGAGC AGTGGGGCAA GCTGTATGAG 
GAGGAGTCCC CCTCCCGCAT GATCATCCAG TACATCCACG ACAACTACTT CCTGGTCAAC 
CTGGTGGACA ACGAGTTTCC GCTGGACAAC TGCCTGTGGC AGGTGGTGGA AGACACGTTT 
GAGCTTCTCA GCAGGCCCCC CCAAGACAAG AGGGAGACAG AGGCGCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005216989.1
CDS	432..2522
Translation	MDCPKPKVFPVGHCCPSLGMRASEAGSGRLSVPPSISRNSAMVNEPRGNGSPGPRWEGSSSGSESSKDSSRCSTPGLDPERCERLREKMKRRMDSGDKWFSLEFFPPRTAQGAVNLISRFDRMGAGGPLFVDVTWHPAGDPGSDKETSSMVIASTAVNYCGLETILHMTCCHQSREEITGHLNKAKQLGLKNILALRGDPIGDQWEEEEGGFNYATDLVKHIRNEFGDYFDVCVAGYPKGHPEGESFEADLKHLKEKVAAGADFIITQLFFEAETFFRFVKACSEIGITCPILPGIFPIQGYHSLRQLVKLSKLEVPQQIKDVIEPIKDNDAAIRNYGIEQAVSLCQELLASGLVPGLHFYTLNREVATIEVLKRLGLWIEDPRRPLPWALSAHPKRRVEDVRPIFWASRPKSYIYRTQEWDEFPNGRWGNSSSPAFGELKDYYLFYLKSKSPKEELLKMWGEELTSEESVFQVFAHYLSGEPNQNGYKVTCLPWNDEPLAAETSLMKEELLRVNRRGILTINSQPNINGKPSSDPIVGWGPSGGYVFQKAYLEFFTSRETVEALLQVLKKYELRVNYHIVDVKGENITNAPELQPNAVTWGIFPGREIIQPTVVDPVSFMFWKDEAFALWIEQWGKLYEEESPSRMIIQYIHDNYFLVNLVDNEFPLDNCLWQVVEDTFELLSRPPQDKRETEAL

Target ORF information:

RefSeq Version	XM_005216932.3
Organism	Bos taurus(cattle)
Definition	Bos taurus methylenetetrahydrofolate reductase (NAD(P)H) (MTHFR), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005216932.3

ORF Insert Sequence:

ATGGACTGCC CAAAACCCAA GGTCTTCCCA GTGGGGCACT GCTGCCCCTC CCTGGGAATG 
CGAGCTTCGG AGGCTGGCAG CGGAAGGCTC TCAGTGCCAC CCTCCATCAG CAGGAACTCG 
GCCATGGTGA ACGAACCCAG AGGGAACGGC AGCCCCGGCC CCCGCTGGGA GGGCAGCAGC 
AGCGGCAGCG AGAGCTCCAA GGACAGTTCA AGGTGCTCCA CGCCGGGGTT GGACCCCGAG 
CGATGCGAGA GACTCCGGGA GAAGATGAAG CGGAGGATGG ACTCTGGAGA CAAGTGGTTC 
TCCCTAGAGT TCTTCCCTCC CCGAACTGCC CAGGGTGCTG TCAATCTCAT CTCCAGGTTT 
GACCGGATGG GAGCGGGTGG CCCCCTCTTT GTAGACGTGA CCTGGCACCC AGCCGGGGAT 
CCTGGCTCCG ACAAGGAGAC CTCGTCCATG GTGATTGCCA GCACTGCCGT GAACTACTGC 
GGCCTCGAGA CAATCCTGCA CATGACCTGC TGCCATCAGA GCCGTGAAGA GATCACGGGC 
CACCTGAACA AAGCCAAGCA GCTGGGCCTG AAGAACATCC TGGCGCTGAG GGGAGACCCC 
ATAGGTGACC AATGGGAAGA GGAGGAAGGA GGCTTCAACT ATGCTACGGA CTTGGTGAAG 
CACATCCGAA ACGAGTTTGG TGACTACTTT GACGTCTGTG TGGCAGGTTA CCCCAAAGGC 
CACCCTGAAG GAGAGAGCTT TGAGGCTGAT CTGAAGCACC TGAAGGAGAA GGTGGCTGCA 
GGAGCCGACT TCATCATCAC CCAGCTATTC TTTGAGGCTG AAACATTCTT CCGCTTTGTG 
AAGGCTTGCT CCGAGATAGG CATTACCTGC CCCATCCTCC CCGGCATCTT CCCAATTCAG 
GGCTACCACT CCCTCCGGCA GCTGGTGAAA CTATCCAAAC TGGAGGTGCC ACAGCAGATC 
AAGGATGTGA TCGAGCCCAT CAAAGACAAT GATGCTGCCA TCCGCAACTA TGGCATCGAG 
CAGGCCGTGA GCCTGTGCCA GGAGCTGCTG GCCAGTGGCT TGGTTCCAGG CCTCCACTTC 
TACACCCTCA ACCGCGAGGT GGCCACCATC GAGGTGCTGA AGCGCCTGGG GCTTTGGATT 
GAGGACCCTA GGCGTCCCCT GCCCTGGGCC CTCAGTGCCC ACCCCAAGCG CCGGGTGGAG 
GATGTCCGGC CCATCTTCTG GGCCTCGAGA CCAAAGAGTT ACATTTACCG TACGCAGGAG 
TGGGACGAGT TCCCCAATGG CCGCTGGGGC AATTCCTCCT CTCCGGCCTT TGGGGAGCTG 
AAGGACTACT ACCTCTTCTA CCTAAAGAGC AAGTCCCCGA AGGAAGAGCT GCTCAAGATG 
TGGGGGGAGG AGCTGACCAG TGAGGAAAGC GTCTTTCAAG TCTTTGCCCA CTACCTCTCG 
GGAGAGCCCA ACCAGAATGG CTACAAAGTG ACTTGCCTGC CCTGGAATGA TGAGCCCCTG 
GCAGCCGAGA CCAGCCTGAT GAAGGAGGAG CTGCTGAGAG TGAACCGGCG GGGCATCCTC 
ACCATCAACT CCCAGCCCAA CATCAACGGG AAGCCGTCCT CTGACCCCAT TGTGGGCTGG 
GGCCCCAGTG GGGGCTATGT CTTCCAGAAG GCCTACTTAG AGTTCTTCAC TTCCCGAGAA 
ACGGTGGAGG CGCTTTTGCA GGTGCTGAAG AAGTATGAAC TCCGGGTGAA TTACCACATC 
GTGGATGTGA AGGGTGAAAA TATCACCAAC GCCCCTGAGC TGCAACCCAA CGCGGTCACC 
TGGGGCATCT TCCCTGGCCG AGAGATCATC CAGCCCACAG TGGTGGACCC GGTCAGCTTC 
ATGTTCTGGA AGGATGAGGC CTTCGCGCTG TGGATTGAGC AGTGGGGCAA GCTGTATGAG 
GAGGAGTCCC CCTCCCGCAT GATCATCCAG TACATCCACG ACAACTACTT CCTGGTCAAC 
CTGGTGGACA ACGAGTTTCC GCTGGACAAC TGCCTGTGGC AGGTGGTGGA AGACACGTTT 
GAGCTTCTCA GCAGGCCCCC CCAAGACAAG AGGGAGACAG AGGCGCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTHFR ( XM_024976080.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024831848.1 Bos taurus methylenetetrahydrofolate reductase (MTHFR), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa223464
Clone ID Related Accession (Same CDS sequence)	XM_024976080.1
Accession Version	XM_024976080.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1779bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	methylenetetrahydrofolate reductase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTGCC CAAAACCCAA GGTCTTCCCA GTGGGGCACT GCTGCCCCTC CCTGGGAATG 
CGAGCTTCGG AGGCTGGCAG CGGAAGGCTC TCAGTGCCAC CCTCCATCAG CAGGAACTCG 
GCCATGGTGA ACGAACCCAG AGGGAACGGC AGCCCCGGCC CCCGCTGGGA GGGCAGCAGC 
AGCGGCAGCG AGAGCTCCAA GGACAGTTCA AGGTGCTCCA CGCCGGGGTT GGACCCCGAG 
CGATGCGAGA GACTCCGGGA GAAGATGAAG CGGAGGATGG ACTCTGGAGA CAAGTGGTTC 
TCCCTAGAGT TCTTCCCTCC CCGAACTGCC CAGGGTGCTG TCAATCTCAT CTCCAGGTTT 
GACCGGATGG GAGCGGGTGG CCCCCTCTTT GTAGACGTGA CCTGGCACCC AGCCGGGGAT 
CCTGGCTCCG ACAAGGAGAC CTCGTCCATG GTGATTGCCA GCACTGCCGT GAACTACTGC 
GGCCTCGAGA CAATCCTGCA CATGACCTGC TGCCATCAGA GCCGTGAAGA GATCACGGGC 
CACCTGAACA AAGCCAAGCA GCTGGGCCTG AAGAACATCC TGGCGCTGAG GGGAGACCCC 
ATAGGTGACC AATGGGAAGA GGAGGAAGGA GGCTTCAACT ATGCTACGGA CTTGGTGAAG 
CACATCCGAA ACGAGTTTGG TGACTACTTT GACGTCTGTG TGGCAGGTTA CCCCAAAGGC 
CACCCTGAAG GAGAGAGCTT TGAGGCTGAT CTGAAGCACC TGAAGGAGAA GGTGGCTGCA 
GGAGCCGACT TCATCATCAC CCAGCTATTC TTTGAGGCTG AAACATTCTT CCGCTTTGTG 
AAGGCTTGCT CCGAGATAGG CATTACCTGC CCCATCCTCC CCGGCATCTT CCCAATTCAG 
GCGTCCCCTG CCCTGGGCCC TCAGTGCCCA CCCCAAGCGC CGGGTGGAGG ATGTCCGGCC 
CATCTTCTGG GCCTCGAGAC CAAAGAGTTA CATTTACCGT ACGCAGGAGT GGGACGAGTT 
CCCCAATGGC CGCTGAGCAA GTCCCCGAAG GAAGAGCTGC TCAAGATGTG GGGGGAGGAG 
CTGACCAGTG AGGAAAGCGT CTTTCAAGTC TTTGCCCACT ACCTCTCGGG AGAGCCCAAC 
CAGAATGGCT ACAAAGTGAC TTGCCTGCCC TGGAATGATG AGCCCCTGGC AGCCGAGACC 
AGCCTGATGA AGGAGGAGCT GCTGAGAGTG AACCGGCGGG GCATCCTCAC CATCAACTCC 
CAGCCCAACA TCAACGGGAA GCCGTCCTCT GACCCCATTG TGGGCTGGGG CCCCAGTGGG 
GGCTATGTCT TCCAGAAGGC CTACTTAGAG TTCTTCACTT CCCGAGAAAC GGTGGAGGCG 
CTTTTGCAGG TGCTGAAGAA GTATGAACTC CGGGTGAATT ACCACATCGT GGATGTGAAG 
GGTGAAAATA TCACCAACGC CCCTGAGCTG CAACCCAACG CGGTCACCTG GGGCATCTTC 
CCTGGCCGAG AGATCATCCA GCCCACAGTG GTGGACCCGG TCAGCTTCAT GTTCTGGAAG 
GATGAGGCCT TCGCGCTGTG GATTGAGCAG TGGGGCAAGC TGTATGAGGA GGAGTCCCCC 
TCCCGCATGA TCATCCAGTA CATCCACGAC AACTACTTCC TGGTCAACCT GGTGGACAAC 
GAGTTTCCGC TGGACAACTG CCTGTGGCAG GTGGTGGAAG ACACGTTTGA GCTTCTCAGC 
AGGCCCCCCC AAGACAAGAG GGAGACAGAG GCGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024831848.1
CDS	101..1879
Translation	MDCPKPKVFPVGHCCPSLGMRASEAGSGRLSVPPSISRNSAMVNEPRGNGSPGPRWEGSSSGSESSKDSSRCSTPGLDPERCERLREKMKRRMDSGDKWFSLEFFPPRTAQGAVNLISRFDRMGAGGPLFVDVTWHPAGDPGSDKETSSMVIASTAVNYCGLETILHMTCCHQSREEITGHLNKAKQLGLKNILALRGDPIGDQWEEEEGGFNYATDLVKHIRNEFGDYFDVCVAGYPKGHPEGESFEADLKHLKEKVAAGADFIITQLFFEAETFFRFVKACSEIGITCPILPGIFPIQASPALGPQCPPQAPGGGCPAHLLGLETKELHLPYAGVGRVPQWPLSKSPKEELLKMWGEELTSEESVFQVFAHYLSGEPNQNGYKVTCLPWNDEPLAAETSLMKEELLRVNRRGILTINSQPNINGKPSSDPIVGWGPSGGYVFQKAYLEFFTSRETVEALLQVLKKYELRVNYHIVDVKGENITNAPELQPNAVTWGIFPGREIIQPTVVDPVSFMFWKDEAFALWIEQWGKLYEEESPSRMIIQYIHDNYFLVNLVDNEFPLDNCLWQVVEDTFELLSRPPQDKRETEAL

Target ORF information:

RefSeq Version	XM_024976080.1
Organism	Bos taurus(cattle)
Definition	Bos taurus methylenetetrahydrofolate reductase (MTHFR), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976080.1

ORF Insert Sequence:

ATGGACTGCC CAAAACCCAA GGTCTTCCCA GTGGGGCACT GCTGCCCCTC CCTGGGAATG 
CGAGCTTCGG AGGCTGGCAG CGGAAGGCTC TCAGTGCCAC CCTCCATCAG CAGGAACTCG 
GCCATGGTGA ACGAACCCAG AGGGAACGGC AGCCCCGGCC CCCGCTGGGA GGGCAGCAGC 
AGCGGCAGCG AGAGCTCCAA GGACAGTTCA AGGTGCTCCA CGCCGGGGTT GGACCCCGAG 
CGATGCGAGA GACTCCGGGA GAAGATGAAG CGGAGGATGG ACTCTGGAGA CAAGTGGTTC 
TCCCTAGAGT TCTTCCCTCC CCGAACTGCC CAGGGTGCTG TCAATCTCAT CTCCAGGTTT 
GACCGGATGG GAGCGGGTGG CCCCCTCTTT GTAGACGTGA CCTGGCACCC AGCCGGGGAT 
CCTGGCTCCG ACAAGGAGAC CTCGTCCATG GTGATTGCCA GCACTGCCGT GAACTACTGC 
GGCCTCGAGA CAATCCTGCA CATGACCTGC TGCCATCAGA GCCGTGAAGA GATCACGGGC 
CACCTGAACA AAGCCAAGCA GCTGGGCCTG AAGAACATCC TGGCGCTGAG GGGAGACCCC 
ATAGGTGACC AATGGGAAGA GGAGGAAGGA GGCTTCAACT ATGCTACGGA CTTGGTGAAG 
CACATCCGAA ACGAGTTTGG TGACTACTTT GACGTCTGTG TGGCAGGTTA CCCCAAAGGC 
CACCCTGAAG GAGAGAGCTT TGAGGCTGAT CTGAAGCACC TGAAGGAGAA GGTGGCTGCA 
GGAGCCGACT TCATCATCAC CCAGCTATTC TTTGAGGCTG AAACATTCTT CCGCTTTGTG 
AAGGCTTGCT CCGAGATAGG CATTACCTGC CCCATCCTCC CCGGCATCTT CCCAATTCAG 
GCGTCCCCTG CCCTGGGCCC TCAGTGCCCA CCCCAAGCGC CGGGTGGAGG ATGTCCGGCC 
CATCTTCTGG GCCTCGAGAC CAAAGAGTTA CATTTACCGT ACGCAGGAGT GGGACGAGTT 
CCCCAATGGC CGCTGAGCAA GTCCCCGAAG GAAGAGCTGC TCAAGATGTG GGGGGAGGAG 
CTGACCAGTG AGGAAAGCGT CTTTCAAGTC TTTGCCCACT ACCTCTCGGG AGAGCCCAAC 
CAGAATGGCT ACAAAGTGAC TTGCCTGCCC TGGAATGATG AGCCCCTGGC AGCCGAGACC 
AGCCTGATGA AGGAGGAGCT GCTGAGAGTG AACCGGCGGG GCATCCTCAC CATCAACTCC 
CAGCCCAACA TCAACGGGAA GCCGTCCTCT GACCCCATTG TGGGCTGGGG CCCCAGTGGG 
GGCTATGTCT TCCAGAAGGC CTACTTAGAG TTCTTCACTT CCCGAGAAAC GGTGGAGGCG 
CTTTTGCAGG TGCTGAAGAA GTATGAACTC CGGGTGAATT ACCACATCGT GGATGTGAAG 
GGTGAAAATA TCACCAACGC CCCTGAGCTG CAACCCAACG CGGTCACCTG GGGCATCTTC 
CCTGGCCGAG AGATCATCCA GCCCACAGTG GTGGACCCGG TCAGCTTCAT GTTCTGGAAG 
GATGAGGCCT TCGCGCTGTG GATTGAGCAG TGGGGCAAGC TGTATGAGGA GGAGTCCCCC 
TCCCGCATGA TCATCCAGTA CATCCACGAC AACTACTTCC TGGTCAACCT GGTGGACAAC 
GAGTTTCCGC TGGACAACTG CCTGTGGCAG GTGGTGGAAG ACACGTTTGA GCTTCTCAGC 
AGGCCCCCCC AAGACAAGAG GGAGACAGAG GCGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTHFR ( XM_015475084.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330570.1 Bos taurus methylenetetrahydrofolate reductase (NAD(P)H) (MTHFR), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa92927
Clone ID Related Accession (Same CDS sequence)	XM_015475084.1 , XM_015475084.2
Accession Version	XM_015475084.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	methylenetetrahydrofolate reductase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104438.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAACG AACCCAGAGG GAACGGCAGC CCCGGCCCCC GCTGGGAGGG CAGCAGCAGC 
GGCAGCGAGA GCTCCAAGGA CAGTTCAAGG TGCTCCACGC CGGGGTTGGA CCCCGAGCGA 
TGCGAGAGAC TCCGGGAGAA GATGAAGCGG AGGATGGACT CTGGAGACAA GTGGTTCTCC 
CTAGAGTTCT TCCCTCCCCG AACTGCCCAG GGTGCTGTCA ATCTCATCTC CAGGTTTGAC 
CGGATGGGAG CGGGTGGCCC CCTCTTTGTA GACGTGACCT GGCACCCAGC CGGGGATCCT 
GGCTCCGACA AGGAGACCTC GTCCATGGTG ATTGCCAGCA CTGCCGTGAA CTACTGCGGC 
CTCGAGACAA TCCTGCACAT GACCTGCTGC CATCAGAGCC GTGAAGAGAT CACGGGCCAC 
CTGAACAAAG CCAAGCAGCT GGGCCTGAAG AACATCCTGG CGCTGAGGGG AGACCCCATA 
GGTGACCAAT GGGAAGAGGA GGAAGGAGGC TTCAACTATG CTACGGACTT GGTGAAGCAC 
ATCCGAAACG AGTTTGGTGA CTACTTTGAC GTCTGTGTGG CAGGTTACCC CAAAGGCCAC 
CCTGAAGGAG AGAGCTTTGA GGCTGATCTG AAGCACCTGA AGGAGAAGGT GGCTGCAGGA 
GCCGACTTCA TCATCACCCA GCTATTCTTT GAGGCTGAAA CATTCTTCCG CTTTGTGAAG 
GCTTGCTCCG AGATAGGCAT TACCTGCCCC ATCCTCCCCG GCATCTTCCC AATTCAGGGC 
TACCACTCCC TCCGGCAGCT GGTGAAACTA TCCAAACTGG AGGTGCCACA GCAGATCAAG 
GATGTGATCG AGCCCATCAA AGACAATGAT GCTGCCATCC GCAACTATGG CATCGAGCAG 
GCCGTGAGCC TGTGCCAGGA GCTGCTGGCC AGTGGCTTGG TTCCAGGCCT CCACTTCTAC 
ACCCTCAACC GCGAGGTGGC CACCATCGAG GTGCTGAAGC GCCTGGGGCT TTGGATTGAG 
GACCCTAGGC GTCCCCTGCC CTGGGCCCTC AGTGCCCACC CCAAGCGCCG GGTGGAGGAT 
GTCCGGCCCA TCTTCTGGGC CTCGAGACCA AAGAGTTACA TTTACCGTAC GCAGGAGTGG 
GACGAGTTCC CCAATGGCCG CTGGGGCAAT TCCTCCTCTC CGGCCTTTGG GGAGCTGAAG 
GACTACTACC TCTTCTACCT AAAGAGCAAG TCCCCGAAGG AAGAGCTGCT CAAGATGTGG 
GGGGAGGAGC TGACCAGTGA GGAAAGCGTC TTTCAAGTCT TTGCCCACTA CCTCTCGGGA 
GAGCCCAACC AGAATGGCTA CAAAGTGACT TGCCTGCCCT GGAATGATGA GCCCCTGGCA 
GCCGAGACCA GCCTGATGAA GGAGGAGCTG CTGAGAGTGA ACCGGCGGGG CATCCTCACC 
ATCAACTCCC AGCCCAACAT CAACGGGAAG CCGTCCTCTG ACCCCATTGT GGGCTGGGGC 
CCCAGTGGGG GCTATGTCTT CCAGAAGGCC TACTTAGAGT TCTTCACTTC CCGAGAAACG 
GTGGAGGCGC TTTTGCAGGT GCTGAAGAAG TATGAACTCC GGGTGAATTA CCACATCGTG 
GATGTGAAGG GTGAAAATAT CACCAACGCC CCTGAGCTGC AACCCAACGC GGTCACCTGG 
GGCATCTTCC CTGGCCGAGA GATCATCCAG CCCACAGTGG TGGACCCGGT CAGCTTCATG 
TTCTGGAAGG ATGAGGCCTT CGCGCTGTGG ATTGAGCAGT GGGGCAAGCT GTATGAGGAG 
GAGTCCCCCT CCCGCATGAT CATCCAGTAC ATCCACGACA ACTACTTCCT GGTCAACCTG 
GTGGACAACG AGTTTCCGCT GGACAACTGC CTGTGGCAGG TGGTGGAAGA CACGTTTGAG 
CTTCTCAGCA GGCCCCCCCA AGACAAGAGG GAGACAGAGG CGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330570.1
CDS	263..2230
Translation	MVNEPRGNGSPGPRWEGSSSGSESSKDSSRCSTPGLDPERCERLREKMKRRMDSGDKWFSLEFFPPRTAQGAVNLISRFDRMGAGGPLFVDVTWHPAGDPGSDKETSSMVIASTAVNYCGLETILHMTCCHQSREEITGHLNKAKQLGLKNILALRGDPIGDQWEEEEGGFNYATDLVKHIRNEFGDYFDVCVAGYPKGHPEGESFEADLKHLKEKVAAGADFIITQLFFEAETFFRFVKACSEIGITCPILPGIFPIQGYHSLRQLVKLSKLEVPQQIKDVIEPIKDNDAAIRNYGIEQAVSLCQELLASGLVPGLHFYTLNREVATIEVLKRLGLWIEDPRRPLPWALSAHPKRRVEDVRPIFWASRPKSYIYRTQEWDEFPNGRWGNSSSPAFGELKDYYLFYLKSKSPKEELLKMWGEELTSEESVFQVFAHYLSGEPNQNGYKVTCLPWNDEPLAAETSLMKEELLRVNRRGILTINSQPNINGKPSSDPIVGWGPSGGYVFQKAYLEFFTSRETVEALLQVLKKYELRVNYHIVDVKGENITNAPELQPNAVTWGIFPGREIIQPTVVDPVSFMFWKDEAFALWIEQWGKLYEEESPSRMIIQYIHDNYFLVNLVDNEFPLDNCLWQVVEDTFELLSRPPQDKRETEAL

Target ORF information:

RefSeq Version	XM_015475084.1
Organism	Bos taurus(cattle)
Definition	Bos taurus methylenetetrahydrofolate reductase (NAD(P)H) (MTHFR), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015475084.1

ORF Insert Sequence:

ATGGTGAACG AACCCAGAGG GAACGGCAGC CCCGGCCCCC GCTGGGAGGG CAGCAGCAGC 
GGCAGCGAGA GCTCCAAGGA CAGTTCAAGG TGCTCCACGC CGGGGTTGGA CCCCGAGCGA 
TGCGAGAGAC TCCGGGAGAA GATGAAGCGG AGGATGGACT CTGGAGACAA GTGGTTCTCC 
CTAGAGTTCT TCCCTCCCCG AACTGCCCAG GGTGCTGTCA ATCTCATCTC CAGGTTTGAC 
CGGATGGGAG CGGGTGGCCC CCTCTTTGTA GACGTGACCT GGCACCCAGC CGGGGATCCT 
GGCTCCGACA AGGAGACCTC GTCCATGGTG ATTGCCAGCA CTGCCGTGAA CTACTGCGGC 
CTCGAGACAA TCCTGCACAT GACCTGCTGC CATCAGAGCC GTGAAGAGAT CACGGGCCAC 
CTGAACAAAG CCAAGCAGCT GGGCCTGAAG AACATCCTGG CGCTGAGGGG AGACCCCATA 
GGTGACCAAT GGGAAGAGGA GGAAGGAGGC TTCAACTATG CTACGGACTT GGTGAAGCAC 
ATCCGAAACG AGTTTGGTGA CTACTTTGAC GTCTGTGTGG CAGGTTACCC CAAAGGCCAC 
CCTGAAGGAG AGAGCTTTGA GGCTGATCTG AAGCACCTGA AGGAGAAGGT GGCTGCAGGA 
GCCGACTTCA TCATCACCCA GCTATTCTTT GAGGCTGAAA CATTCTTCCG CTTTGTGAAG 
GCTTGCTCCG AGATAGGCAT TACCTGCCCC ATCCTCCCCG GCATCTTCCC AATTCAGGGC 
TACCACTCCC TCCGGCAGCT GGTGAAACTA TCCAAACTGG AGGTGCCACA GCAGATCAAG 
GATGTGATCG AGCCCATCAA AGACAATGAT GCTGCCATCC GCAACTATGG CATCGAGCAG 
GCCGTGAGCC TGTGCCAGGA GCTGCTGGCC AGTGGCTTGG TTCCAGGCCT CCACTTCTAC 
ACCCTCAACC GCGAGGTGGC CACCATCGAG GTGCTGAAGC GCCTGGGGCT TTGGATTGAG 
GACCCTAGGC GTCCCCTGCC CTGGGCCCTC AGTGCCCACC CCAAGCGCCG GGTGGAGGAT 
GTCCGGCCCA TCTTCTGGGC CTCGAGACCA AAGAGTTACA TTTACCGTAC GCAGGAGTGG 
GACGAGTTCC CCAATGGCCG CTGGGGCAAT TCCTCCTCTC CGGCCTTTGG GGAGCTGAAG 
GACTACTACC TCTTCTACCT AAAGAGCAAG TCCCCGAAGG AAGAGCTGCT CAAGATGTGG 
GGGGAGGAGC TGACCAGTGA GGAAAGCGTC TTTCAAGTCT TTGCCCACTA CCTCTCGGGA 
GAGCCCAACC AGAATGGCTA CAAAGTGACT TGCCTGCCCT GGAATGATGA GCCCCTGGCA 
GCCGAGACCA GCCTGATGAA GGAGGAGCTG CTGAGAGTGA ACCGGCGGGG CATCCTCACC 
ATCAACTCCC AGCCCAACAT CAACGGGAAG CCGTCCTCTG ACCCCATTGT GGGCTGGGGC 
CCCAGTGGGG GCTATGTCTT CCAGAAGGCC TACTTAGAGT TCTTCACTTC CCGAGAAACG 
GTGGAGGCGC TTTTGCAGGT GCTGAAGAAG TATGAACTCC GGGTGAATTA CCACATCGTG 
GATGTGAAGG GTGAAAATAT CACCAACGCC CCTGAGCTGC AACCCAACGC GGTCACCTGG 
GGCATCTTCC CTGGCCGAGA GATCATCCAG CCCACAGTGG TGGACCCGGT CAGCTTCATG 
TTCTGGAAGG ATGAGGCCTT CGCGCTGTGG ATTGAGCAGT GGGGCAAGCT GTATGAGGAG 
GAGTCCCCCT CCCGCATGAT CATCCAGTAC ATCCACGACA ACTACTTCCT GGTCAACCTG 
GTGGACAACG AGTTTCCGCT GGACAACTGC CTGTGGCAGG TGGTGGAAGA CACGTTTGAG 
CTTCTCAGCA GGCCCCCCCA AGACAAGAGG GAGACAGAGG CGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTHFR ( XM_015475084.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330570.1 Bos taurus methylenetetrahydrofolate reductase (MTHFR), transcript variant X2, mRNA.

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CloneID	OBa92927
Clone ID Related Accession (Same CDS sequence)	XM_015475084.1 , XM_015475084.2
Accession Version	XM_015475084.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	methylenetetrahydrofolate reductase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015475084.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAACG AACCCAGAGG GAACGGCAGC CCCGGCCCCC GCTGGGAGGG CAGCAGCAGC 
GGCAGCGAGA GCTCCAAGGA CAGTTCAAGG TGCTCCACGC CGGGGTTGGA CCCCGAGCGA 
TGCGAGAGAC TCCGGGAGAA GATGAAGCGG AGGATGGACT CTGGAGACAA GTGGTTCTCC 
CTAGAGTTCT TCCCTCCCCG AACTGCCCAG GGTGCTGTCA ATCTCATCTC CAGGTTTGAC 
CGGATGGGAG CGGGTGGCCC CCTCTTTGTA GACGTGACCT GGCACCCAGC CGGGGATCCT 
GGCTCCGACA AGGAGACCTC GTCCATGGTG ATTGCCAGCA CTGCCGTGAA CTACTGCGGC 
CTCGAGACAA TCCTGCACAT GACCTGCTGC CATCAGAGCC GTGAAGAGAT CACGGGCCAC 
CTGAACAAAG CCAAGCAGCT GGGCCTGAAG AACATCCTGG CGCTGAGGGG AGACCCCATA 
GGTGACCAAT GGGAAGAGGA GGAAGGAGGC TTCAACTATG CTACGGACTT GGTGAAGCAC 
ATCCGAAACG AGTTTGGTGA CTACTTTGAC GTCTGTGTGG CAGGTTACCC CAAAGGCCAC 
CCTGAAGGAG AGAGCTTTGA GGCTGATCTG AAGCACCTGA AGGAGAAGGT GGCTGCAGGA 
GCCGACTTCA TCATCACCCA GCTATTCTTT GAGGCTGAAA CATTCTTCCG CTTTGTGAAG 
GCTTGCTCCG AGATAGGCAT TACCTGCCCC ATCCTCCCCG GCATCTTCCC AATTCAGGGC 
TACCACTCCC TCCGGCAGCT GGTGAAACTA TCCAAACTGG AGGTGCCACA GCAGATCAAG 
GATGTGATCG AGCCCATCAA AGACAATGAT GCTGCCATCC GCAACTATGG CATCGAGCAG 
GCCGTGAGCC TGTGCCAGGA GCTGCTGGCC AGTGGCTTGG TTCCAGGCCT CCACTTCTAC 
ACCCTCAACC GCGAGGTGGC CACCATCGAG GTGCTGAAGC GCCTGGGGCT TTGGATTGAG 
GACCCTAGGC GTCCCCTGCC CTGGGCCCTC AGTGCCCACC CCAAGCGCCG GGTGGAGGAT 
GTCCGGCCCA TCTTCTGGGC CTCGAGACCA AAGAGTTACA TTTACCGTAC GCAGGAGTGG 
GACGAGTTCC CCAATGGCCG CTGGGGCAAT TCCTCCTCTC CGGCCTTTGG GGAGCTGAAG 
GACTACTACC TCTTCTACCT AAAGAGCAAG TCCCCGAAGG AAGAGCTGCT CAAGATGTGG 
GGGGAGGAGC TGACCAGTGA GGAAAGCGTC TTTCAAGTCT TTGCCCACTA CCTCTCGGGA 
GAGCCCAACC AGAATGGCTA CAAAGTGACT TGCCTGCCCT GGAATGATGA GCCCCTGGCA 
GCCGAGACCA GCCTGATGAA GGAGGAGCTG CTGAGAGTGA ACCGGCGGGG CATCCTCACC 
ATCAACTCCC AGCCCAACAT CAACGGGAAG CCGTCCTCTG ACCCCATTGT GGGCTGGGGC 
CCCAGTGGGG GCTATGTCTT CCAGAAGGCC TACTTAGAGT TCTTCACTTC CCGAGAAACG 
GTGGAGGCGC TTTTGCAGGT GCTGAAGAAG TATGAACTCC GGGTGAATTA CCACATCGTG 
GATGTGAAGG GTGAAAATAT CACCAACGCC CCTGAGCTGC AACCCAACGC GGTCACCTGG 
GGCATCTTCC CTGGCCGAGA GATCATCCAG CCCACAGTGG TGGACCCGGT CAGCTTCATG 
TTCTGGAAGG ATGAGGCCTT CGCGCTGTGG ATTGAGCAGT GGGGCAAGCT GTATGAGGAG 
GAGTCCCCCT CCCGCATGAT CATCCAGTAC ATCCACGACA ACTACTTCCT GGTCAACCTG 
GTGGACAACG AGTTTCCGCT GGACAACTGC CTGTGGCAGG TGGTGGAAGA CACGTTTGAG 
CTTCTCAGCA GGCCCCCCCA AGACAAGAGG GAGACAGAGG CGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330570.1
CDS	331..2298
Translation	MVNEPRGNGSPGPRWEGSSSGSESSKDSSRCSTPGLDPERCERLREKMKRRMDSGDKWFSLEFFPPRTAQGAVNLISRFDRMGAGGPLFVDVTWHPAGDPGSDKETSSMVIASTAVNYCGLETILHMTCCHQSREEITGHLNKAKQLGLKNILALRGDPIGDQWEEEEGGFNYATDLVKHIRNEFGDYFDVCVAGYPKGHPEGESFEADLKHLKEKVAAGADFIITQLFFEAETFFRFVKACSEIGITCPILPGIFPIQGYHSLRQLVKLSKLEVPQQIKDVIEPIKDNDAAIRNYGIEQAVSLCQELLASGLVPGLHFYTLNREVATIEVLKRLGLWIEDPRRPLPWALSAHPKRRVEDVRPIFWASRPKSYIYRTQEWDEFPNGRWGNSSSPAFGELKDYYLFYLKSKSPKEELLKMWGEELTSEESVFQVFAHYLSGEPNQNGYKVTCLPWNDEPLAAETSLMKEELLRVNRRGILTINSQPNINGKPSSDPIVGWGPSGGYVFQKAYLEFFTSRETVEALLQVLKKYELRVNYHIVDVKGENITNAPELQPNAVTWGIFPGREIIQPTVVDPVSFMFWKDEAFALWIEQWGKLYEEESPSRMIIQYIHDNYFLVNLVDNEFPLDNCLWQVVEDTFELLSRPPQDKRETEAL

Target ORF information:

RefSeq Version	XM_015475084.2
Organism	Bos taurus(cattle)
Definition	Bos taurus methylenetetrahydrofolate reductase (MTHFR), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015475084.2

ORF Insert Sequence:

ATGGTGAACG AACCCAGAGG GAACGGCAGC CCCGGCCCCC GCTGGGAGGG CAGCAGCAGC 
GGCAGCGAGA GCTCCAAGGA CAGTTCAAGG TGCTCCACGC CGGGGTTGGA CCCCGAGCGA 
TGCGAGAGAC TCCGGGAGAA GATGAAGCGG AGGATGGACT CTGGAGACAA GTGGTTCTCC 
CTAGAGTTCT TCCCTCCCCG AACTGCCCAG GGTGCTGTCA ATCTCATCTC CAGGTTTGAC 
CGGATGGGAG CGGGTGGCCC CCTCTTTGTA GACGTGACCT GGCACCCAGC CGGGGATCCT 
GGCTCCGACA AGGAGACCTC GTCCATGGTG ATTGCCAGCA CTGCCGTGAA CTACTGCGGC 
CTCGAGACAA TCCTGCACAT GACCTGCTGC CATCAGAGCC GTGAAGAGAT CACGGGCCAC 
CTGAACAAAG CCAAGCAGCT GGGCCTGAAG AACATCCTGG CGCTGAGGGG AGACCCCATA 
GGTGACCAAT GGGAAGAGGA GGAAGGAGGC TTCAACTATG CTACGGACTT GGTGAAGCAC 
ATCCGAAACG AGTTTGGTGA CTACTTTGAC GTCTGTGTGG CAGGTTACCC CAAAGGCCAC 
CCTGAAGGAG AGAGCTTTGA GGCTGATCTG AAGCACCTGA AGGAGAAGGT GGCTGCAGGA 
GCCGACTTCA TCATCACCCA GCTATTCTTT GAGGCTGAAA CATTCTTCCG CTTTGTGAAG 
GCTTGCTCCG AGATAGGCAT TACCTGCCCC ATCCTCCCCG GCATCTTCCC AATTCAGGGC 
TACCACTCCC TCCGGCAGCT GGTGAAACTA TCCAAACTGG AGGTGCCACA GCAGATCAAG 
GATGTGATCG AGCCCATCAA AGACAATGAT GCTGCCATCC GCAACTATGG CATCGAGCAG 
GCCGTGAGCC TGTGCCAGGA GCTGCTGGCC AGTGGCTTGG TTCCAGGCCT CCACTTCTAC 
ACCCTCAACC GCGAGGTGGC CACCATCGAG GTGCTGAAGC GCCTGGGGCT TTGGATTGAG 
GACCCTAGGC GTCCCCTGCC CTGGGCCCTC AGTGCCCACC CCAAGCGCCG GGTGGAGGAT 
GTCCGGCCCA TCTTCTGGGC CTCGAGACCA AAGAGTTACA TTTACCGTAC GCAGGAGTGG 
GACGAGTTCC CCAATGGCCG CTGGGGCAAT TCCTCCTCTC CGGCCTTTGG GGAGCTGAAG 
GACTACTACC TCTTCTACCT AAAGAGCAAG TCCCCGAAGG AAGAGCTGCT CAAGATGTGG 
GGGGAGGAGC TGACCAGTGA GGAAAGCGTC TTTCAAGTCT TTGCCCACTA CCTCTCGGGA 
GAGCCCAACC AGAATGGCTA CAAAGTGACT TGCCTGCCCT GGAATGATGA GCCCCTGGCA 
GCCGAGACCA GCCTGATGAA GGAGGAGCTG CTGAGAGTGA ACCGGCGGGG CATCCTCACC 
ATCAACTCCC AGCCCAACAT CAACGGGAAG CCGTCCTCTG ACCCCATTGT GGGCTGGGGC 
CCCAGTGGGG GCTATGTCTT CCAGAAGGCC TACTTAGAGT TCTTCACTTC CCGAGAAACG 
GTGGAGGCGC TTTTGCAGGT GCTGAAGAAG TATGAACTCC GGGTGAATTA CCACATCGTG 
GATGTGAAGG GTGAAAATAT CACCAACGCC CCTGAGCTGC AACCCAACGC GGTCACCTGG 
GGCATCTTCC CTGGCCGAGA GATCATCCAG CCCACAGTGG TGGACCCGGT CAGCTTCATG 
TTCTGGAAGG ATGAGGCCTT CGCGCTGTGG ATTGAGCAGT GGGGCAAGCT GTATGAGGAG 
GAGTCCCCCT CCCGCATGAT CATCCAGTAC ATCCACGACA ACTACTTCCT GGTCAACCTG 
GTGGACAACG AGTTTCCGCT GGACAACTGC CTGTGGCAGG TGGTGGAAGA CACGTTTGAG 
CTTCTCAGCA GGCCCCCCCA AGACAAGAGG GAGACAGAGG CGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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MTHFR cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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