ATP2B4 cDNA ORF clone, Bos taurus(cattle)

The following ATP2B4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ATP2B4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa223428 XM_024976045.1
Latest version!
Bos taurus ATPase plasma membrane Ca2+ transporting 4 (ATP2B4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa223428 XM_024976042.1
Latest version!
Bos taurus ATPase plasma membrane Ca2+ transporting 4 (ATP2B4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa223428 XM_024976041.1
Latest version!
Bos taurus ATPase plasma membrane Ca2+ transporting 4 (ATP2B4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa223428 XM_024976044.1
Latest version!
Bos taurus ATPase plasma membrane Ca2+ transporting 4 (ATP2B4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa223429 XM_024976046.1
Latest version!
Bos taurus ATPase plasma membrane Ca2+ transporting 4 (ATP2B4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa223430 XM_024976047.1
Latest version!
Bos taurus ATPase plasma membrane Ca2+ transporting 4 (ATP2B4), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa223431 XM_024976048.1
Latest version!
Bos taurus ATPase plasma membrane Ca2+ transporting 4 (ATP2B4), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa223432 XM_024976049.1
Latest version!
Bos taurus ATPase plasma membrane Ca2+ transporting 4 (ATP2B4), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa223433 XM_024976050.1
Latest version!
Bos taurus ATPase plasma membrane Ca2+ transporting 4 (ATP2B4), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa223434 XM_024976051.1
Latest version!
Bos taurus ATPase plasma membrane Ca2+ transporting 4 (ATP2B4), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa39523 NM_001172594.1
Latest version!
Bos taurus ATPase plasma membrane Ca2+ transporting 4 (ATP2B4), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa223428
    Clone ID Related Accession (Same CDS sequence) XM_024976041.1 , XM_024976044.1 , XM_024976045.1 , XM_024976042.1
    Accession Version XM_024976041.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3624bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product plasma membrane calcium-transporting ATPase 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGACGAACC CAACAGAACA CACCTTGCCG TCCAACTCGA TACTTGAGAG CCGTGAAGGG 
    GAGTTTGGCT GCACAGTGAT GGACCTGAGG AAGCTGATGG AGCTGCGCTC AAGTGATGCA
    ATAGACCAGA TCAACGTCCA CTACGGAGGA GTGATGAACC TCTGCAGTAG ACTGAAAACC
    AACCCTGTGG AAGGCCTATC TGGGAACCCT GCAGATCTGG AGAAACGTAA ACAAGTGTTT
    GGACAGAACT TGATTCCCCC CAAAAAGCCC AAGACCTTCT TAGAGTTAGT GTGGGAAGCC
    CTCCAGGATG TCACGCTCAT CATCCTGGAG ATTGCGGCCA TCATCTCCCT GGTCCTGTCC
    TTCTACCGCC CCCCGGGGGG AGAAAATGAA CAGTGTGGTC TAGCCGTCAC TAGCCCAGAG
    GACGAAGGGG AGGCGGAAGC TGGCTGGATT GAGGGTGCAG CCATCTTATT CTCGGTGATC
    ATCGTCGTGC TCGTGACCGC CTTCAATGAC TGGAGCAAGG AGAAGCAATT CCGGGGGCTG
    CAGAACCGCA TCGAAAAGGA GCAGAAATTC TCCGTTATCC GGAATGGCCA CATCATTCAG
    CTCCCTGTGG CTGAGATTGT GGTGGGCGAC ATTGCCCAAA TCAAATACGG TGACCTGCTG
    CCTGCAGATG GGATCCTGAT CCAGGGCAAC GATCTCAAGA TTGATGAGAG TTCTCTGACT
    GGGGAATCAG ACCATGTCAA GAAGTCCTTG GAGAGGGACC CCATGCTGCT CTCAGGGACC
    CATGTAATGG AAGGTTCTGG ACGAATGGTA GTGACGGCTG TGGGTATCAA CTCTCAGACT
    GGAATCATTT TTACCCTCTT GGGAGCCAGT GAGGGGGAAG AGGAAGAGAA GAAGAAGAAA
    GGTAAAAAAC AAGGAGTCCC TGAAAATCGC AACAAAGCAA AGACCCAAGA TGGAGTGGCC
    TTGGAAATCC AGCCACTCAA CAGCCAGGAG GGGATCGACA GTGAGGAAAA GGAGAAGAAG
    GCCGCCAAGC TGCCCAAAAA GGAGAAGTCC GTGCTGCAGG GCAAGCTGAC CCGCCTGGCT
    GTCCAGATTG GGAAAGCTGG TCTGATCATG TCTGCAATCA CAGTTCTCAT CCTGATTCTG
    TACTTCGTGA TCGACAACTT TGTGATCCAG CGCAGACCGT GGCTGGCCGA GTGTACCCCC
    ATCTACGTCC AGTACTTTGT CAAGTTCTTC ATCATCGGCG TCACGGTGCT GGTGGTGGCT
    GTGCCCGAGG GGCTGCCCCT GGCCGTCACC ATCTCACTGG CTTACTCTGT GAAGAAAATG
    ATGAAGGACA ACAACCTGGT GCGGCACTTG GACGCGTGTG AGACCATGGG CAACGCCACA
    GCCATCTGCT CGGACAAGAC GGGCACGCTG ACCATGAACC GCATGTCTGT GGTGCAGGCT
    TACATTGGAG ACACTCGTTA CCATCAGATC CCAAGCCCTG ATGACCTTGT GCCCAAGGTC
    CTGGACCTTA TTGTCAATGG CATTTCCATC AACAGTGCCT ACACCTCTAA GATTCTGCCT
    CCGGAGAAGG AGGGCGGCCT GCCCCGGCAG GTGGGCAACA AGACCGAGTG CGCCCTGCTG
    GGCTTCGTCT CGGACCTGAA GCAGGATTAC CACGCCGTGC GCAGCGAGGT GCCCGAGGAG
    AAGCTCTACA AAGTGTACAC CTTCAACTCG GTGCGCAAGT CCATGAGCAC GGTCATCGAG
    AAGCCGGGCG GCGGCTACCG CATGTACAGC AAGGGCGCCT CCGAGATCAT CCTGCGCAAG
    TGTAATCGAA TCCTGGACAA AAAGGGGGAG GCCGTGCCAT TCAAGAATAA GGACCGAGAT
    GAGATGGTCC GCACCGTCAT CGAGCCCATG GCCTGTGAGG GGCTGCGGAC ACTCTGCATC
    GCTTACCGGG ATTTCAACGA TGGAGAGCCC CCGTGGGACA ACGAGAGCGA GATCCTCACT
    GAGTTGACCT GTATCGCAGT GGTGGGCATC GAGGACCCCG TGCGCCCGGA GGTGCCAGAA
    GCTATTGCCA AATGCAAGCG AGCTGGCATC ACTGTCAGGA TGGTGACAGG GGACAACATC
    AACACAGCCC GTGCCATCGC CACCAAGTGT GGCATCGTGA CTCCCGGGGA TGACTTTCTG
    TGCTTAGAAG GCAAAGAATT CAATCGCCTC ATCCGAAACG AGAAGGGCGA GGTAGAGCAG
    GAAAAGCTGG ACAAGATCTG GCCTAAGCTC CGAGTGCTGG CGCGGTCTTC CCCCACTGAC
    AAGCACACGC TGGTGAAAGG CATCATCGAC AGCACTGTCG GCGACCAGCG GCAGGTGGTG
    GCCGTCACCG GCGACGGCAC CAACGACGGC CCGGCTCTGA AGAAAGCAGA CGTTGGTTTT
    GCCATGGGTA TTGCAGGCAC AGATGTGGCA AAGGAGGCCT CCGACATCAT CCTAACAGAC
    GACAACTTCA CCAGCATCGT GAAGGCGGTG ATGTGGGGAC GGAACGTCTA CGACAGCATC
    TCCAAGTTCC TGCAGTTCCA GCTCACCGTC AACGTGGTGG CCGTGATCGT GGCCTTCACT
    GGAGCCTGCA TCACCCAGGA TTCCCCACTG AAGGCCGTGC AGATGTTGTG GGTTAATCTA
    ATCATGGACA CCTTTGCCTC GCTGGCCCTG GCCACAGAGC CTCCTACAGA CTCTCTACTG
    AAGCGCCGGC CCTACGGCCG CAATAAGCCT CTAATCTCGC GCACCATGAT GAAGAACATC
    CTGGGTCACG CCGTCTATCA GCTCACCGTC ATCTTTTTCC TTGTGTTTGC GGGTGAGAAA
    TTCTTTGACA TTGACAGCGG GAGGAGAGCG CCTCTGCATT CTCCGCCCAG CCAGCACTAC
    ACCATTATTT TCAACACCTT CGTGCTCATG CAGCTCTTCA ACGAAATCAA CTCCCGCAAG
    ATCCACGGGG AGAGGAATGT CTTCTCGGGC ATCTTCCGCA ATCTCATCTT CTGCTCAGTG
    GTGTTGGGCA CATTCATCAG CCAGATCATC ATCGTGGAAT TCGGGGGCAA ACCCTTCAGT
    TGCACGAAGC TCACCCTGTC CCAGTGGTTC TGGTGCCTCT TCATTGGTAT TGGAGAGCTG
    CTGTGGGGCC AGGTCATTTC CACGATACCT ACCCAATCCC TGAAGTTCCT GAAGGAGGCT
    GGGCATGGCA CCACCAAAGA GGAGATCACC AAGGATGCTG AGGGGCTGGA CGAAATCGAC
    CATGCAGAGA TGGAGCTGCG CCGAGGCCAG ATCCTCTGGT TCCGGGGCCT GAACCGTATC
    CAGACTCAGA TCAAAGTGGT CAAAGCGTTC CATAGTTCCC TCCACGAAAG CATTCAGAAA
    CCCAAGAACC AAAACTCCAT CCACAACTTC ATGACCCACC CTGAATTCAC CATAGATGAG
    GAGGGGCCAC GAACACCACT CCTGGATGAG CAAGAAGAGG AGATTTTTGA AAAGGTGTCT
    AAGCCTGGGA CTAAGACGTC CTCACTGGAT GGTGAGGTCA CTCCACAAAC CAACAAAAAC
    AACAATACGG TGGATTGCTG CCAAGTACAA ATTGTTGCTT CCCACTCGGA CAGCCCTCTA
    CACAGCCTGG AGACGTCCGT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831809.1
    CDS924..4547
    Translation

    Target ORF information:

    RefSeq Version XM_024976041.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ATPase plasma membrane Ca2+ transporting 4 (ATP2B4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976041.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGACGAACC CAACAGAACA CACCTTGCCG TCCAACTCGA TACTTGAGAG CCGTGAAGGG 
    GAGTTTGGCT GCACAGTGAT GGACCTGAGG AAGCTGATGG AGCTGCGCTC AAGTGATGCA
    ATAGACCAGA TCAACGTCCA CTACGGAGGA GTGATGAACC TCTGCAGTAG ACTGAAAACC
    AACCCTGTGG AAGGCCTATC TGGGAACCCT GCAGATCTGG AGAAACGTAA ACAAGTGTTT
    GGACAGAACT TGATTCCCCC CAAAAAGCCC AAGACCTTCT TAGAGTTAGT GTGGGAAGCC
    CTCCAGGATG TCACGCTCAT CATCCTGGAG ATTGCGGCCA TCATCTCCCT GGTCCTGTCC
    TTCTACCGCC CCCCGGGGGG AGAAAATGAA CAGTGTGGTC TAGCCGTCAC TAGCCCAGAG
    GACGAAGGGG AGGCGGAAGC TGGCTGGATT GAGGGTGCAG CCATCTTATT CTCGGTGATC
    ATCGTCGTGC TCGTGACCGC CTTCAATGAC TGGAGCAAGG AGAAGCAATT CCGGGGGCTG
    CAGAACCGCA TCGAAAAGGA GCAGAAATTC TCCGTTATCC GGAATGGCCA CATCATTCAG
    CTCCCTGTGG CTGAGATTGT GGTGGGCGAC ATTGCCCAAA TCAAATACGG TGACCTGCTG
    CCTGCAGATG GGATCCTGAT CCAGGGCAAC GATCTCAAGA TTGATGAGAG TTCTCTGACT
    GGGGAATCAG ACCATGTCAA GAAGTCCTTG GAGAGGGACC CCATGCTGCT CTCAGGGACC
    CATGTAATGG AAGGTTCTGG ACGAATGGTA GTGACGGCTG TGGGTATCAA CTCTCAGACT
    GGAATCATTT TTACCCTCTT GGGAGCCAGT GAGGGGGAAG AGGAAGAGAA GAAGAAGAAA
    GGTAAAAAAC AAGGAGTCCC TGAAAATCGC AACAAAGCAA AGACCCAAGA TGGAGTGGCC
    TTGGAAATCC AGCCACTCAA CAGCCAGGAG GGGATCGACA GTGAGGAAAA GGAGAAGAAG
    GCCGCCAAGC TGCCCAAAAA GGAGAAGTCC GTGCTGCAGG GCAAGCTGAC CCGCCTGGCT
    GTCCAGATTG GGAAAGCTGG TCTGATCATG TCTGCAATCA CAGTTCTCAT CCTGATTCTG
    TACTTCGTGA TCGACAACTT TGTGATCCAG CGCAGACCGT GGCTGGCCGA GTGTACCCCC
    ATCTACGTCC AGTACTTTGT CAAGTTCTTC ATCATCGGCG TCACGGTGCT GGTGGTGGCT
    GTGCCCGAGG GGCTGCCCCT GGCCGTCACC ATCTCACTGG CTTACTCTGT GAAGAAAATG
    ATGAAGGACA ACAACCTGGT GCGGCACTTG GACGCGTGTG AGACCATGGG CAACGCCACA
    GCCATCTGCT CGGACAAGAC GGGCACGCTG ACCATGAACC GCATGTCTGT GGTGCAGGCT
    TACATTGGAG ACACTCGTTA CCATCAGATC CCAAGCCCTG ATGACCTTGT GCCCAAGGTC
    CTGGACCTTA TTGTCAATGG CATTTCCATC AACAGTGCCT ACACCTCTAA GATTCTGCCT
    CCGGAGAAGG AGGGCGGCCT GCCCCGGCAG GTGGGCAACA AGACCGAGTG CGCCCTGCTG
    GGCTTCGTCT CGGACCTGAA GCAGGATTAC CACGCCGTGC GCAGCGAGGT GCCCGAGGAG
    AAGCTCTACA AAGTGTACAC CTTCAACTCG GTGCGCAAGT CCATGAGCAC GGTCATCGAG
    AAGCCGGGCG GCGGCTACCG CATGTACAGC AAGGGCGCCT CCGAGATCAT CCTGCGCAAG
    TGTAATCGAA TCCTGGACAA AAAGGGGGAG GCCGTGCCAT TCAAGAATAA GGACCGAGAT
    GAGATGGTCC GCACCGTCAT CGAGCCCATG GCCTGTGAGG GGCTGCGGAC ACTCTGCATC
    GCTTACCGGG ATTTCAACGA TGGAGAGCCC CCGTGGGACA ACGAGAGCGA GATCCTCACT
    GAGTTGACCT GTATCGCAGT GGTGGGCATC GAGGACCCCG TGCGCCCGGA GGTGCCAGAA
    GCTATTGCCA AATGCAAGCG AGCTGGCATC ACTGTCAGGA TGGTGACAGG GGACAACATC
    AACACAGCCC GTGCCATCGC CACCAAGTGT GGCATCGTGA CTCCCGGGGA TGACTTTCTG
    TGCTTAGAAG GCAAAGAATT CAATCGCCTC ATCCGAAACG AGAAGGGCGA GGTAGAGCAG
    GAAAAGCTGG ACAAGATCTG GCCTAAGCTC CGAGTGCTGG CGCGGTCTTC CCCCACTGAC
    AAGCACACGC TGGTGAAAGG CATCATCGAC AGCACTGTCG GCGACCAGCG GCAGGTGGTG
    GCCGTCACCG GCGACGGCAC CAACGACGGC CCGGCTCTGA AGAAAGCAGA CGTTGGTTTT
    GCCATGGGTA TTGCAGGCAC AGATGTGGCA AAGGAGGCCT CCGACATCAT CCTAACAGAC
    GACAACTTCA CCAGCATCGT GAAGGCGGTG ATGTGGGGAC GGAACGTCTA CGACAGCATC
    TCCAAGTTCC TGCAGTTCCA GCTCACCGTC AACGTGGTGG CCGTGATCGT GGCCTTCACT
    GGAGCCTGCA TCACCCAGGA TTCCCCACTG AAGGCCGTGC AGATGTTGTG GGTTAATCTA
    ATCATGGACA CCTTTGCCTC GCTGGCCCTG GCCACAGAGC CTCCTACAGA CTCTCTACTG
    AAGCGCCGGC CCTACGGCCG CAATAAGCCT CTAATCTCGC GCACCATGAT GAAGAACATC
    CTGGGTCACG CCGTCTATCA GCTCACCGTC ATCTTTTTCC TTGTGTTTGC GGGTGAGAAA
    TTCTTTGACA TTGACAGCGG GAGGAGAGCG CCTCTGCATT CTCCGCCCAG CCAGCACTAC
    ACCATTATTT TCAACACCTT CGTGCTCATG CAGCTCTTCA ACGAAATCAA CTCCCGCAAG
    ATCCACGGGG AGAGGAATGT CTTCTCGGGC ATCTTCCGCA ATCTCATCTT CTGCTCAGTG
    GTGTTGGGCA CATTCATCAG CCAGATCATC ATCGTGGAAT TCGGGGGCAA ACCCTTCAGT
    TGCACGAAGC TCACCCTGTC CCAGTGGTTC TGGTGCCTCT TCATTGGTAT TGGAGAGCTG
    CTGTGGGGCC AGGTCATTTC CACGATACCT ACCCAATCCC TGAAGTTCCT GAAGGAGGCT
    GGGCATGGCA CCACCAAAGA GGAGATCACC AAGGATGCTG AGGGGCTGGA CGAAATCGAC
    CATGCAGAGA TGGAGCTGCG CCGAGGCCAG ATCCTCTGGT TCCGGGGCCT GAACCGTATC
    CAGACTCAGA TCAAAGTGGT CAAAGCGTTC CATAGTTCCC TCCACGAAAG CATTCAGAAA
    CCCAAGAACC AAAACTCCAT CCACAACTTC ATGACCCACC CTGAATTCAC CATAGATGAG
    GAGGGGCCAC GAACACCACT CCTGGATGAG CAAGAAGAGG AGATTTTTGA AAAGGTGTCT
    AAGCCTGGGA CTAAGACGTC CTCACTGGAT GGTGAGGTCA CTCCACAAAC CAACAAAAAC
    AACAATACGG TGGATTGCTG CCAAGTACAA ATTGTTGCTT CCCACTCGGA CAGCCCTCTA
    CACAGCCTGG AGACGTCCGT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223428
    Clone ID Related Accession (Same CDS sequence) XM_024976041.1 , XM_024976044.1 , XM_024976045.1 , XM_024976042.1
    Accession Version XM_024976044.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3624bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product plasma membrane calcium-transporting ATPase 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGACGAACC CAACAGAACA CACCTTGCCG TCCAACTCGA TACTTGAGAG CCGTGAAGGG 
    GAGTTTGGCT GCACAGTGAT GGACCTGAGG AAGCTGATGG AGCTGCGCTC AAGTGATGCA
    ATAGACCAGA TCAACGTCCA CTACGGAGGA GTGATGAACC TCTGCAGTAG ACTGAAAACC
    AACCCTGTGG AAGGCCTATC TGGGAACCCT GCAGATCTGG AGAAACGTAA ACAAGTGTTT
    GGACAGAACT TGATTCCCCC CAAAAAGCCC AAGACCTTCT TAGAGTTAGT GTGGGAAGCC
    CTCCAGGATG TCACGCTCAT CATCCTGGAG ATTGCGGCCA TCATCTCCCT GGTCCTGTCC
    TTCTACCGCC CCCCGGGGGG AGAAAATGAA CAGTGTGGTC TAGCCGTCAC TAGCCCAGAG
    GACGAAGGGG AGGCGGAAGC TGGCTGGATT GAGGGTGCAG CCATCTTATT CTCGGTGATC
    ATCGTCGTGC TCGTGACCGC CTTCAATGAC TGGAGCAAGG AGAAGCAATT CCGGGGGCTG
    CAGAACCGCA TCGAAAAGGA GCAGAAATTC TCCGTTATCC GGAATGGCCA CATCATTCAG
    CTCCCTGTGG CTGAGATTGT GGTGGGCGAC ATTGCCCAAA TCAAATACGG TGACCTGCTG
    CCTGCAGATG GGATCCTGAT CCAGGGCAAC GATCTCAAGA TTGATGAGAG TTCTCTGACT
    GGGGAATCAG ACCATGTCAA GAAGTCCTTG GAGAGGGACC CCATGCTGCT CTCAGGGACC
    CATGTAATGG AAGGTTCTGG ACGAATGGTA GTGACGGCTG TGGGTATCAA CTCTCAGACT
    GGAATCATTT TTACCCTCTT GGGAGCCAGT GAGGGGGAAG AGGAAGAGAA GAAGAAGAAA
    GGTAAAAAAC AAGGAGTCCC TGAAAATCGC AACAAAGCAA AGACCCAAGA TGGAGTGGCC
    TTGGAAATCC AGCCACTCAA CAGCCAGGAG GGGATCGACA GTGAGGAAAA GGAGAAGAAG
    GCCGCCAAGC TGCCCAAAAA GGAGAAGTCC GTGCTGCAGG GCAAGCTGAC CCGCCTGGCT
    GTCCAGATTG GGAAAGCTGG TCTGATCATG TCTGCAATCA CAGTTCTCAT CCTGATTCTG
    TACTTCGTGA TCGACAACTT TGTGATCCAG CGCAGACCGT GGCTGGCCGA GTGTACCCCC
    ATCTACGTCC AGTACTTTGT CAAGTTCTTC ATCATCGGCG TCACGGTGCT GGTGGTGGCT
    GTGCCCGAGG GGCTGCCCCT GGCCGTCACC ATCTCACTGG CTTACTCTGT GAAGAAAATG
    ATGAAGGACA ACAACCTGGT GCGGCACTTG GACGCGTGTG AGACCATGGG CAACGCCACA
    GCCATCTGCT CGGACAAGAC GGGCACGCTG ACCATGAACC GCATGTCTGT GGTGCAGGCT
    TACATTGGAG ACACTCGTTA CCATCAGATC CCAAGCCCTG ATGACCTTGT GCCCAAGGTC
    CTGGACCTTA TTGTCAATGG CATTTCCATC AACAGTGCCT ACACCTCTAA GATTCTGCCT
    CCGGAGAAGG AGGGCGGCCT GCCCCGGCAG GTGGGCAACA AGACCGAGTG CGCCCTGCTG
    GGCTTCGTCT CGGACCTGAA GCAGGATTAC CACGCCGTGC GCAGCGAGGT GCCCGAGGAG
    AAGCTCTACA AAGTGTACAC CTTCAACTCG GTGCGCAAGT CCATGAGCAC GGTCATCGAG
    AAGCCGGGCG GCGGCTACCG CATGTACAGC AAGGGCGCCT CCGAGATCAT CCTGCGCAAG
    TGTAATCGAA TCCTGGACAA AAAGGGGGAG GCCGTGCCAT TCAAGAATAA GGACCGAGAT
    GAGATGGTCC GCACCGTCAT CGAGCCCATG GCCTGTGAGG GGCTGCGGAC ACTCTGCATC
    GCTTACCGGG ATTTCAACGA TGGAGAGCCC CCGTGGGACA ACGAGAGCGA GATCCTCACT
    GAGTTGACCT GTATCGCAGT GGTGGGCATC GAGGACCCCG TGCGCCCGGA GGTGCCAGAA
    GCTATTGCCA AATGCAAGCG AGCTGGCATC ACTGTCAGGA TGGTGACAGG GGACAACATC
    AACACAGCCC GTGCCATCGC CACCAAGTGT GGCATCGTGA CTCCCGGGGA TGACTTTCTG
    TGCTTAGAAG GCAAAGAATT CAATCGCCTC ATCCGAAACG AGAAGGGCGA GGTAGAGCAG
    GAAAAGCTGG ACAAGATCTG GCCTAAGCTC CGAGTGCTGG CGCGGTCTTC CCCCACTGAC
    AAGCACACGC TGGTGAAAGG CATCATCGAC AGCACTGTCG GCGACCAGCG GCAGGTGGTG
    GCCGTCACCG GCGACGGCAC CAACGACGGC CCGGCTCTGA AGAAAGCAGA CGTTGGTTTT
    GCCATGGGTA TTGCAGGCAC AGATGTGGCA AAGGAGGCCT CCGACATCAT CCTAACAGAC
    GACAACTTCA CCAGCATCGT GAAGGCGGTG ATGTGGGGAC GGAACGTCTA CGACAGCATC
    TCCAAGTTCC TGCAGTTCCA GCTCACCGTC AACGTGGTGG CCGTGATCGT GGCCTTCACT
    GGAGCCTGCA TCACCCAGGA TTCCCCACTG AAGGCCGTGC AGATGTTGTG GGTTAATCTA
    ATCATGGACA CCTTTGCCTC GCTGGCCCTG GCCACAGAGC CTCCTACAGA CTCTCTACTG
    AAGCGCCGGC CCTACGGCCG CAATAAGCCT CTAATCTCGC GCACCATGAT GAAGAACATC
    CTGGGTCACG CCGTCTATCA GCTCACCGTC ATCTTTTTCC TTGTGTTTGC GGGTGAGAAA
    TTCTTTGACA TTGACAGCGG GAGGAGAGCG CCTCTGCATT CTCCGCCCAG CCAGCACTAC
    ACCATTATTT TCAACACCTT CGTGCTCATG CAGCTCTTCA ACGAAATCAA CTCCCGCAAG
    ATCCACGGGG AGAGGAATGT CTTCTCGGGC ATCTTCCGCA ATCTCATCTT CTGCTCAGTG
    GTGTTGGGCA CATTCATCAG CCAGATCATC ATCGTGGAAT TCGGGGGCAA ACCCTTCAGT
    TGCACGAAGC TCACCCTGTC CCAGTGGTTC TGGTGCCTCT TCATTGGTAT TGGAGAGCTG
    CTGTGGGGCC AGGTCATTTC CACGATACCT ACCCAATCCC TGAAGTTCCT GAAGGAGGCT
    GGGCATGGCA CCACCAAAGA GGAGATCACC AAGGATGCTG AGGGGCTGGA CGAAATCGAC
    CATGCAGAGA TGGAGCTGCG CCGAGGCCAG ATCCTCTGGT TCCGGGGCCT GAACCGTATC
    CAGACTCAGA TCAAAGTGGT CAAAGCGTTC CATAGTTCCC TCCACGAAAG CATTCAGAAA
    CCCAAGAACC AAAACTCCAT CCACAACTTC ATGACCCACC CTGAATTCAC CATAGATGAG
    GAGGGGCCAC GAACACCACT CCTGGATGAG CAAGAAGAGG AGATTTTTGA AAAGGTGTCT
    AAGCCTGGGA CTAAGACGTC CTCACTGGAT GGTGAGGTCA CTCCACAAAC CAACAAAAAC
    AACAATACGG TGGATTGCTG CCAAGTACAA ATTGTTGCTT CCCACTCGGA CAGCCCTCTA
    CACAGCCTGG AGACGTCCGT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831812.1
    CDS832..4455
    Translation

    Target ORF information:

    RefSeq Version XM_024976044.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ATPase plasma membrane Ca2+ transporting 4 (ATP2B4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976044.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGACGAACC CAACAGAACA CACCTTGCCG TCCAACTCGA TACTTGAGAG CCGTGAAGGG 
    GAGTTTGGCT GCACAGTGAT GGACCTGAGG AAGCTGATGG AGCTGCGCTC AAGTGATGCA
    ATAGACCAGA TCAACGTCCA CTACGGAGGA GTGATGAACC TCTGCAGTAG ACTGAAAACC
    AACCCTGTGG AAGGCCTATC TGGGAACCCT GCAGATCTGG AGAAACGTAA ACAAGTGTTT
    GGACAGAACT TGATTCCCCC CAAAAAGCCC AAGACCTTCT TAGAGTTAGT GTGGGAAGCC
    CTCCAGGATG TCACGCTCAT CATCCTGGAG ATTGCGGCCA TCATCTCCCT GGTCCTGTCC
    TTCTACCGCC CCCCGGGGGG AGAAAATGAA CAGTGTGGTC TAGCCGTCAC TAGCCCAGAG
    GACGAAGGGG AGGCGGAAGC TGGCTGGATT GAGGGTGCAG CCATCTTATT CTCGGTGATC
    ATCGTCGTGC TCGTGACCGC CTTCAATGAC TGGAGCAAGG AGAAGCAATT CCGGGGGCTG
    CAGAACCGCA TCGAAAAGGA GCAGAAATTC TCCGTTATCC GGAATGGCCA CATCATTCAG
    CTCCCTGTGG CTGAGATTGT GGTGGGCGAC ATTGCCCAAA TCAAATACGG TGACCTGCTG
    CCTGCAGATG GGATCCTGAT CCAGGGCAAC GATCTCAAGA TTGATGAGAG TTCTCTGACT
    GGGGAATCAG ACCATGTCAA GAAGTCCTTG GAGAGGGACC CCATGCTGCT CTCAGGGACC
    CATGTAATGG AAGGTTCTGG ACGAATGGTA GTGACGGCTG TGGGTATCAA CTCTCAGACT
    GGAATCATTT TTACCCTCTT GGGAGCCAGT GAGGGGGAAG AGGAAGAGAA GAAGAAGAAA
    GGTAAAAAAC AAGGAGTCCC TGAAAATCGC AACAAAGCAA AGACCCAAGA TGGAGTGGCC
    TTGGAAATCC AGCCACTCAA CAGCCAGGAG GGGATCGACA GTGAGGAAAA GGAGAAGAAG
    GCCGCCAAGC TGCCCAAAAA GGAGAAGTCC GTGCTGCAGG GCAAGCTGAC CCGCCTGGCT
    GTCCAGATTG GGAAAGCTGG TCTGATCATG TCTGCAATCA CAGTTCTCAT CCTGATTCTG
    TACTTCGTGA TCGACAACTT TGTGATCCAG CGCAGACCGT GGCTGGCCGA GTGTACCCCC
    ATCTACGTCC AGTACTTTGT CAAGTTCTTC ATCATCGGCG TCACGGTGCT GGTGGTGGCT
    GTGCCCGAGG GGCTGCCCCT GGCCGTCACC ATCTCACTGG CTTACTCTGT GAAGAAAATG
    ATGAAGGACA ACAACCTGGT GCGGCACTTG GACGCGTGTG AGACCATGGG CAACGCCACA
    GCCATCTGCT CGGACAAGAC GGGCACGCTG ACCATGAACC GCATGTCTGT GGTGCAGGCT
    TACATTGGAG ACACTCGTTA CCATCAGATC CCAAGCCCTG ATGACCTTGT GCCCAAGGTC
    CTGGACCTTA TTGTCAATGG CATTTCCATC AACAGTGCCT ACACCTCTAA GATTCTGCCT
    CCGGAGAAGG AGGGCGGCCT GCCCCGGCAG GTGGGCAACA AGACCGAGTG CGCCCTGCTG
    GGCTTCGTCT CGGACCTGAA GCAGGATTAC CACGCCGTGC GCAGCGAGGT GCCCGAGGAG
    AAGCTCTACA AAGTGTACAC CTTCAACTCG GTGCGCAAGT CCATGAGCAC GGTCATCGAG
    AAGCCGGGCG GCGGCTACCG CATGTACAGC AAGGGCGCCT CCGAGATCAT CCTGCGCAAG
    TGTAATCGAA TCCTGGACAA AAAGGGGGAG GCCGTGCCAT TCAAGAATAA GGACCGAGAT
    GAGATGGTCC GCACCGTCAT CGAGCCCATG GCCTGTGAGG GGCTGCGGAC ACTCTGCATC
    GCTTACCGGG ATTTCAACGA TGGAGAGCCC CCGTGGGACA ACGAGAGCGA GATCCTCACT
    GAGTTGACCT GTATCGCAGT GGTGGGCATC GAGGACCCCG TGCGCCCGGA GGTGCCAGAA
    GCTATTGCCA AATGCAAGCG AGCTGGCATC ACTGTCAGGA TGGTGACAGG GGACAACATC
    AACACAGCCC GTGCCATCGC CACCAAGTGT GGCATCGTGA CTCCCGGGGA TGACTTTCTG
    TGCTTAGAAG GCAAAGAATT CAATCGCCTC ATCCGAAACG AGAAGGGCGA GGTAGAGCAG
    GAAAAGCTGG ACAAGATCTG GCCTAAGCTC CGAGTGCTGG CGCGGTCTTC CCCCACTGAC
    AAGCACACGC TGGTGAAAGG CATCATCGAC AGCACTGTCG GCGACCAGCG GCAGGTGGTG
    GCCGTCACCG GCGACGGCAC CAACGACGGC CCGGCTCTGA AGAAAGCAGA CGTTGGTTTT
    GCCATGGGTA TTGCAGGCAC AGATGTGGCA AAGGAGGCCT CCGACATCAT CCTAACAGAC
    GACAACTTCA CCAGCATCGT GAAGGCGGTG ATGTGGGGAC GGAACGTCTA CGACAGCATC
    TCCAAGTTCC TGCAGTTCCA GCTCACCGTC AACGTGGTGG CCGTGATCGT GGCCTTCACT
    GGAGCCTGCA TCACCCAGGA TTCCCCACTG AAGGCCGTGC AGATGTTGTG GGTTAATCTA
    ATCATGGACA CCTTTGCCTC GCTGGCCCTG GCCACAGAGC CTCCTACAGA CTCTCTACTG
    AAGCGCCGGC CCTACGGCCG CAATAAGCCT CTAATCTCGC GCACCATGAT GAAGAACATC
    CTGGGTCACG CCGTCTATCA GCTCACCGTC ATCTTTTTCC TTGTGTTTGC GGGTGAGAAA
    TTCTTTGACA TTGACAGCGG GAGGAGAGCG CCTCTGCATT CTCCGCCCAG CCAGCACTAC
    ACCATTATTT TCAACACCTT CGTGCTCATG CAGCTCTTCA ACGAAATCAA CTCCCGCAAG
    ATCCACGGGG AGAGGAATGT CTTCTCGGGC ATCTTCCGCA ATCTCATCTT CTGCTCAGTG
    GTGTTGGGCA CATTCATCAG CCAGATCATC ATCGTGGAAT TCGGGGGCAA ACCCTTCAGT
    TGCACGAAGC TCACCCTGTC CCAGTGGTTC TGGTGCCTCT TCATTGGTAT TGGAGAGCTG
    CTGTGGGGCC AGGTCATTTC CACGATACCT ACCCAATCCC TGAAGTTCCT GAAGGAGGCT
    GGGCATGGCA CCACCAAAGA GGAGATCACC AAGGATGCTG AGGGGCTGGA CGAAATCGAC
    CATGCAGAGA TGGAGCTGCG CCGAGGCCAG ATCCTCTGGT TCCGGGGCCT GAACCGTATC
    CAGACTCAGA TCAAAGTGGT CAAAGCGTTC CATAGTTCCC TCCACGAAAG CATTCAGAAA
    CCCAAGAACC AAAACTCCAT CCACAACTTC ATGACCCACC CTGAATTCAC CATAGATGAG
    GAGGGGCCAC GAACACCACT CCTGGATGAG CAAGAAGAGG AGATTTTTGA AAAGGTGTCT
    AAGCCTGGGA CTAAGACGTC CTCACTGGAT GGTGAGGTCA CTCCACAAAC CAACAAAAAC
    AACAATACGG TGGATTGCTG CCAAGTACAA ATTGTTGCTT CCCACTCGGA CAGCCCTCTA
    CACAGCCTGG AGACGTCCGT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223428
    Clone ID Related Accession (Same CDS sequence) XM_024976041.1 , XM_024976044.1 , XM_024976045.1 , XM_024976042.1
    Accession Version XM_024976045.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3624bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product plasma membrane calcium-transporting ATPase 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGACGAACC CAACAGAACA CACCTTGCCG TCCAACTCGA TACTTGAGAG CCGTGAAGGG 
    GAGTTTGGCT GCACAGTGAT GGACCTGAGG AAGCTGATGG AGCTGCGCTC AAGTGATGCA
    ATAGACCAGA TCAACGTCCA CTACGGAGGA GTGATGAACC TCTGCAGTAG ACTGAAAACC
    AACCCTGTGG AAGGCCTATC TGGGAACCCT GCAGATCTGG AGAAACGTAA ACAAGTGTTT
    GGACAGAACT TGATTCCCCC CAAAAAGCCC AAGACCTTCT TAGAGTTAGT GTGGGAAGCC
    CTCCAGGATG TCACGCTCAT CATCCTGGAG ATTGCGGCCA TCATCTCCCT GGTCCTGTCC
    TTCTACCGCC CCCCGGGGGG AGAAAATGAA CAGTGTGGTC TAGCCGTCAC TAGCCCAGAG
    GACGAAGGGG AGGCGGAAGC TGGCTGGATT GAGGGTGCAG CCATCTTATT CTCGGTGATC
    ATCGTCGTGC TCGTGACCGC CTTCAATGAC TGGAGCAAGG AGAAGCAATT CCGGGGGCTG
    CAGAACCGCA TCGAAAAGGA GCAGAAATTC TCCGTTATCC GGAATGGCCA CATCATTCAG
    CTCCCTGTGG CTGAGATTGT GGTGGGCGAC ATTGCCCAAA TCAAATACGG TGACCTGCTG
    CCTGCAGATG GGATCCTGAT CCAGGGCAAC GATCTCAAGA TTGATGAGAG TTCTCTGACT
    GGGGAATCAG ACCATGTCAA GAAGTCCTTG GAGAGGGACC CCATGCTGCT CTCAGGGACC
    CATGTAATGG AAGGTTCTGG ACGAATGGTA GTGACGGCTG TGGGTATCAA CTCTCAGACT
    GGAATCATTT TTACCCTCTT GGGAGCCAGT GAGGGGGAAG AGGAAGAGAA GAAGAAGAAA
    GGTAAAAAAC AAGGAGTCCC TGAAAATCGC AACAAAGCAA AGACCCAAGA TGGAGTGGCC
    TTGGAAATCC AGCCACTCAA CAGCCAGGAG GGGATCGACA GTGAGGAAAA GGAGAAGAAG
    GCCGCCAAGC TGCCCAAAAA GGAGAAGTCC GTGCTGCAGG GCAAGCTGAC CCGCCTGGCT
    GTCCAGATTG GGAAAGCTGG TCTGATCATG TCTGCAATCA CAGTTCTCAT CCTGATTCTG
    TACTTCGTGA TCGACAACTT TGTGATCCAG CGCAGACCGT GGCTGGCCGA GTGTACCCCC
    ATCTACGTCC AGTACTTTGT CAAGTTCTTC ATCATCGGCG TCACGGTGCT GGTGGTGGCT
    GTGCCCGAGG GGCTGCCCCT GGCCGTCACC ATCTCACTGG CTTACTCTGT GAAGAAAATG
    ATGAAGGACA ACAACCTGGT GCGGCACTTG GACGCGTGTG AGACCATGGG CAACGCCACA
    GCCATCTGCT CGGACAAGAC GGGCACGCTG ACCATGAACC GCATGTCTGT GGTGCAGGCT
    TACATTGGAG ACACTCGTTA CCATCAGATC CCAAGCCCTG ATGACCTTGT GCCCAAGGTC
    CTGGACCTTA TTGTCAATGG CATTTCCATC AACAGTGCCT ACACCTCTAA GATTCTGCCT
    CCGGAGAAGG AGGGCGGCCT GCCCCGGCAG GTGGGCAACA AGACCGAGTG CGCCCTGCTG
    GGCTTCGTCT CGGACCTGAA GCAGGATTAC CACGCCGTGC GCAGCGAGGT GCCCGAGGAG
    AAGCTCTACA AAGTGTACAC CTTCAACTCG GTGCGCAAGT CCATGAGCAC GGTCATCGAG
    AAGCCGGGCG GCGGCTACCG CATGTACAGC AAGGGCGCCT CCGAGATCAT CCTGCGCAAG
    TGTAATCGAA TCCTGGACAA AAAGGGGGAG GCCGTGCCAT TCAAGAATAA GGACCGAGAT
    GAGATGGTCC GCACCGTCAT CGAGCCCATG GCCTGTGAGG GGCTGCGGAC ACTCTGCATC
    GCTTACCGGG ATTTCAACGA TGGAGAGCCC CCGTGGGACA ACGAGAGCGA GATCCTCACT
    GAGTTGACCT GTATCGCAGT GGTGGGCATC GAGGACCCCG TGCGCCCGGA GGTGCCAGAA
    GCTATTGCCA AATGCAAGCG AGCTGGCATC ACTGTCAGGA TGGTGACAGG GGACAACATC
    AACACAGCCC GTGCCATCGC CACCAAGTGT GGCATCGTGA CTCCCGGGGA TGACTTTCTG
    TGCTTAGAAG GCAAAGAATT CAATCGCCTC ATCCGAAACG AGAAGGGCGA GGTAGAGCAG
    GAAAAGCTGG ACAAGATCTG GCCTAAGCTC CGAGTGCTGG CGCGGTCTTC CCCCACTGAC
    AAGCACACGC TGGTGAAAGG CATCATCGAC AGCACTGTCG GCGACCAGCG GCAGGTGGTG
    GCCGTCACCG GCGACGGCAC CAACGACGGC CCGGCTCTGA AGAAAGCAGA CGTTGGTTTT
    GCCATGGGTA TTGCAGGCAC AGATGTGGCA AAGGAGGCCT CCGACATCAT CCTAACAGAC
    GACAACTTCA CCAGCATCGT GAAGGCGGTG ATGTGGGGAC GGAACGTCTA CGACAGCATC
    TCCAAGTTCC TGCAGTTCCA GCTCACCGTC AACGTGGTGG CCGTGATCGT GGCCTTCACT
    GGAGCCTGCA TCACCCAGGA TTCCCCACTG AAGGCCGTGC AGATGTTGTG GGTTAATCTA
    ATCATGGACA CCTTTGCCTC GCTGGCCCTG GCCACAGAGC CTCCTACAGA CTCTCTACTG
    AAGCGCCGGC CCTACGGCCG CAATAAGCCT CTAATCTCGC GCACCATGAT GAAGAACATC
    CTGGGTCACG CCGTCTATCA GCTCACCGTC ATCTTTTTCC TTGTGTTTGC GGGTGAGAAA
    TTCTTTGACA TTGACAGCGG GAGGAGAGCG CCTCTGCATT CTCCGCCCAG CCAGCACTAC
    ACCATTATTT TCAACACCTT CGTGCTCATG CAGCTCTTCA ACGAAATCAA CTCCCGCAAG
    ATCCACGGGG AGAGGAATGT CTTCTCGGGC ATCTTCCGCA ATCTCATCTT CTGCTCAGTG
    GTGTTGGGCA CATTCATCAG CCAGATCATC ATCGTGGAAT TCGGGGGCAA ACCCTTCAGT
    TGCACGAAGC TCACCCTGTC CCAGTGGTTC TGGTGCCTCT TCATTGGTAT TGGAGAGCTG
    CTGTGGGGCC AGGTCATTTC CACGATACCT ACCCAATCCC TGAAGTTCCT GAAGGAGGCT
    GGGCATGGCA CCACCAAAGA GGAGATCACC AAGGATGCTG AGGGGCTGGA CGAAATCGAC
    CATGCAGAGA TGGAGCTGCG CCGAGGCCAG ATCCTCTGGT TCCGGGGCCT GAACCGTATC
    CAGACTCAGA TCAAAGTGGT CAAAGCGTTC CATAGTTCCC TCCACGAAAG CATTCAGAAA
    CCCAAGAACC AAAACTCCAT CCACAACTTC ATGACCCACC CTGAATTCAC CATAGATGAG
    GAGGGGCCAC GAACACCACT CCTGGATGAG CAAGAAGAGG AGATTTTTGA AAAGGTGTCT
    AAGCCTGGGA CTAAGACGTC CTCACTGGAT GGTGAGGTCA CTCCACAAAC CAACAAAAAC
    AACAATACGG TGGATTGCTG CCAAGTACAA ATTGTTGCTT CCCACTCGGA CAGCCCTCTA
    CACAGCCTGG AGACGTCCGT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831813.1
    CDS588..4211
    Translation

    Target ORF information:

    RefSeq Version XM_024976045.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ATPase plasma membrane Ca2+ transporting 4 (ATP2B4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976045.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGACGAACC CAACAGAACA CACCTTGCCG TCCAACTCGA TACTTGAGAG CCGTGAAGGG 
    GAGTTTGGCT GCACAGTGAT GGACCTGAGG AAGCTGATGG AGCTGCGCTC AAGTGATGCA
    ATAGACCAGA TCAACGTCCA CTACGGAGGA GTGATGAACC TCTGCAGTAG ACTGAAAACC
    AACCCTGTGG AAGGCCTATC TGGGAACCCT GCAGATCTGG AGAAACGTAA ACAAGTGTTT
    GGACAGAACT TGATTCCCCC CAAAAAGCCC AAGACCTTCT TAGAGTTAGT GTGGGAAGCC
    CTCCAGGATG TCACGCTCAT CATCCTGGAG ATTGCGGCCA TCATCTCCCT GGTCCTGTCC
    TTCTACCGCC CCCCGGGGGG AGAAAATGAA CAGTGTGGTC TAGCCGTCAC TAGCCCAGAG
    GACGAAGGGG AGGCGGAAGC TGGCTGGATT GAGGGTGCAG CCATCTTATT CTCGGTGATC
    ATCGTCGTGC TCGTGACCGC CTTCAATGAC TGGAGCAAGG AGAAGCAATT CCGGGGGCTG
    CAGAACCGCA TCGAAAAGGA GCAGAAATTC TCCGTTATCC GGAATGGCCA CATCATTCAG
    CTCCCTGTGG CTGAGATTGT GGTGGGCGAC ATTGCCCAAA TCAAATACGG TGACCTGCTG
    CCTGCAGATG GGATCCTGAT CCAGGGCAAC GATCTCAAGA TTGATGAGAG TTCTCTGACT
    GGGGAATCAG ACCATGTCAA GAAGTCCTTG GAGAGGGACC CCATGCTGCT CTCAGGGACC
    CATGTAATGG AAGGTTCTGG ACGAATGGTA GTGACGGCTG TGGGTATCAA CTCTCAGACT
    GGAATCATTT TTACCCTCTT GGGAGCCAGT GAGGGGGAAG AGGAAGAGAA GAAGAAGAAA
    GGTAAAAAAC AAGGAGTCCC TGAAAATCGC AACAAAGCAA AGACCCAAGA TGGAGTGGCC
    TTGGAAATCC AGCCACTCAA CAGCCAGGAG GGGATCGACA GTGAGGAAAA GGAGAAGAAG
    GCCGCCAAGC TGCCCAAAAA GGAGAAGTCC GTGCTGCAGG GCAAGCTGAC CCGCCTGGCT
    GTCCAGATTG GGAAAGCTGG TCTGATCATG TCTGCAATCA CAGTTCTCAT CCTGATTCTG
    TACTTCGTGA TCGACAACTT TGTGATCCAG CGCAGACCGT GGCTGGCCGA GTGTACCCCC
    ATCTACGTCC AGTACTTTGT CAAGTTCTTC ATCATCGGCG TCACGGTGCT GGTGGTGGCT
    GTGCCCGAGG GGCTGCCCCT GGCCGTCACC ATCTCACTGG CTTACTCTGT GAAGAAAATG
    ATGAAGGACA ACAACCTGGT GCGGCACTTG GACGCGTGTG AGACCATGGG CAACGCCACA
    GCCATCTGCT CGGACAAGAC GGGCACGCTG ACCATGAACC GCATGTCTGT GGTGCAGGCT
    TACATTGGAG ACACTCGTTA CCATCAGATC CCAAGCCCTG ATGACCTTGT GCCCAAGGTC
    CTGGACCTTA TTGTCAATGG CATTTCCATC AACAGTGCCT ACACCTCTAA GATTCTGCCT
    CCGGAGAAGG AGGGCGGCCT GCCCCGGCAG GTGGGCAACA AGACCGAGTG CGCCCTGCTG
    GGCTTCGTCT CGGACCTGAA GCAGGATTAC CACGCCGTGC GCAGCGAGGT GCCCGAGGAG
    AAGCTCTACA AAGTGTACAC CTTCAACTCG GTGCGCAAGT CCATGAGCAC GGTCATCGAG
    AAGCCGGGCG GCGGCTACCG CATGTACAGC AAGGGCGCCT CCGAGATCAT CCTGCGCAAG
    TGTAATCGAA TCCTGGACAA AAAGGGGGAG GCCGTGCCAT TCAAGAATAA GGACCGAGAT
    GAGATGGTCC GCACCGTCAT CGAGCCCATG GCCTGTGAGG GGCTGCGGAC ACTCTGCATC
    GCTTACCGGG ATTTCAACGA TGGAGAGCCC CCGTGGGACA ACGAGAGCGA GATCCTCACT
    GAGTTGACCT GTATCGCAGT GGTGGGCATC GAGGACCCCG TGCGCCCGGA GGTGCCAGAA
    GCTATTGCCA AATGCAAGCG AGCTGGCATC ACTGTCAGGA TGGTGACAGG GGACAACATC
    AACACAGCCC GTGCCATCGC CACCAAGTGT GGCATCGTGA CTCCCGGGGA TGACTTTCTG
    TGCTTAGAAG GCAAAGAATT CAATCGCCTC ATCCGAAACG AGAAGGGCGA GGTAGAGCAG
    GAAAAGCTGG ACAAGATCTG GCCTAAGCTC CGAGTGCTGG CGCGGTCTTC CCCCACTGAC
    AAGCACACGC TGGTGAAAGG CATCATCGAC AGCACTGTCG GCGACCAGCG GCAGGTGGTG
    GCCGTCACCG GCGACGGCAC CAACGACGGC CCGGCTCTGA AGAAAGCAGA CGTTGGTTTT
    GCCATGGGTA TTGCAGGCAC AGATGTGGCA AAGGAGGCCT CCGACATCAT CCTAACAGAC
    GACAACTTCA CCAGCATCGT GAAGGCGGTG ATGTGGGGAC GGAACGTCTA CGACAGCATC
    TCCAAGTTCC TGCAGTTCCA GCTCACCGTC AACGTGGTGG CCGTGATCGT GGCCTTCACT
    GGAGCCTGCA TCACCCAGGA TTCCCCACTG AAGGCCGTGC AGATGTTGTG GGTTAATCTA
    ATCATGGACA CCTTTGCCTC GCTGGCCCTG GCCACAGAGC CTCCTACAGA CTCTCTACTG
    AAGCGCCGGC CCTACGGCCG CAATAAGCCT CTAATCTCGC GCACCATGAT GAAGAACATC
    CTGGGTCACG CCGTCTATCA GCTCACCGTC ATCTTTTTCC TTGTGTTTGC GGGTGAGAAA
    TTCTTTGACA TTGACAGCGG GAGGAGAGCG CCTCTGCATT CTCCGCCCAG CCAGCACTAC
    ACCATTATTT TCAACACCTT CGTGCTCATG CAGCTCTTCA ACGAAATCAA CTCCCGCAAG
    ATCCACGGGG AGAGGAATGT CTTCTCGGGC ATCTTCCGCA ATCTCATCTT CTGCTCAGTG
    GTGTTGGGCA CATTCATCAG CCAGATCATC ATCGTGGAAT TCGGGGGCAA ACCCTTCAGT
    TGCACGAAGC TCACCCTGTC CCAGTGGTTC TGGTGCCTCT TCATTGGTAT TGGAGAGCTG
    CTGTGGGGCC AGGTCATTTC CACGATACCT ACCCAATCCC TGAAGTTCCT GAAGGAGGCT
    GGGCATGGCA CCACCAAAGA GGAGATCACC AAGGATGCTG AGGGGCTGGA CGAAATCGAC
    CATGCAGAGA TGGAGCTGCG CCGAGGCCAG ATCCTCTGGT TCCGGGGCCT GAACCGTATC
    CAGACTCAGA TCAAAGTGGT CAAAGCGTTC CATAGTTCCC TCCACGAAAG CATTCAGAAA
    CCCAAGAACC AAAACTCCAT CCACAACTTC ATGACCCACC CTGAATTCAC CATAGATGAG
    GAGGGGCCAC GAACACCACT CCTGGATGAG CAAGAAGAGG AGATTTTTGA AAAGGTGTCT
    AAGCCTGGGA CTAAGACGTC CTCACTGGAT GGTGAGGTCA CTCCACAAAC CAACAAAAAC
    AACAATACGG TGGATTGCTG CCAAGTACAA ATTGTTGCTT CCCACTCGGA CAGCCCTCTA
    CACAGCCTGG AGACGTCCGT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223428
    Clone ID Related Accession (Same CDS sequence) XM_024976041.1 , XM_024976044.1 , XM_024976045.1 , XM_024976042.1
    Accession Version XM_024976042.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3624bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product plasma membrane calcium-transporting ATPase 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGACGAACC CAACAGAACA CACCTTGCCG TCCAACTCGA TACTTGAGAG CCGTGAAGGG 
    GAGTTTGGCT GCACAGTGAT GGACCTGAGG AAGCTGATGG AGCTGCGCTC AAGTGATGCA
    ATAGACCAGA TCAACGTCCA CTACGGAGGA GTGATGAACC TCTGCAGTAG ACTGAAAACC
    AACCCTGTGG AAGGCCTATC TGGGAACCCT GCAGATCTGG AGAAACGTAA ACAAGTGTTT
    GGACAGAACT TGATTCCCCC CAAAAAGCCC AAGACCTTCT TAGAGTTAGT GTGGGAAGCC
    CTCCAGGATG TCACGCTCAT CATCCTGGAG ATTGCGGCCA TCATCTCCCT GGTCCTGTCC
    TTCTACCGCC CCCCGGGGGG AGAAAATGAA CAGTGTGGTC TAGCCGTCAC TAGCCCAGAG
    GACGAAGGGG AGGCGGAAGC TGGCTGGATT GAGGGTGCAG CCATCTTATT CTCGGTGATC
    ATCGTCGTGC TCGTGACCGC CTTCAATGAC TGGAGCAAGG AGAAGCAATT CCGGGGGCTG
    CAGAACCGCA TCGAAAAGGA GCAGAAATTC TCCGTTATCC GGAATGGCCA CATCATTCAG
    CTCCCTGTGG CTGAGATTGT GGTGGGCGAC ATTGCCCAAA TCAAATACGG TGACCTGCTG
    CCTGCAGATG GGATCCTGAT CCAGGGCAAC GATCTCAAGA TTGATGAGAG TTCTCTGACT
    GGGGAATCAG ACCATGTCAA GAAGTCCTTG GAGAGGGACC CCATGCTGCT CTCAGGGACC
    CATGTAATGG AAGGTTCTGG ACGAATGGTA GTGACGGCTG TGGGTATCAA CTCTCAGACT
    GGAATCATTT TTACCCTCTT GGGAGCCAGT GAGGGGGAAG AGGAAGAGAA GAAGAAGAAA
    GGTAAAAAAC AAGGAGTCCC TGAAAATCGC AACAAAGCAA AGACCCAAGA TGGAGTGGCC
    TTGGAAATCC AGCCACTCAA CAGCCAGGAG GGGATCGACA GTGAGGAAAA GGAGAAGAAG
    GCCGCCAAGC TGCCCAAAAA GGAGAAGTCC GTGCTGCAGG GCAAGCTGAC CCGCCTGGCT
    GTCCAGATTG GGAAAGCTGG TCTGATCATG TCTGCAATCA CAGTTCTCAT CCTGATTCTG
    TACTTCGTGA TCGACAACTT TGTGATCCAG CGCAGACCGT GGCTGGCCGA GTGTACCCCC
    ATCTACGTCC AGTACTTTGT CAAGTTCTTC ATCATCGGCG TCACGGTGCT GGTGGTGGCT
    GTGCCCGAGG GGCTGCCCCT GGCCGTCACC ATCTCACTGG CTTACTCTGT GAAGAAAATG
    ATGAAGGACA ACAACCTGGT GCGGCACTTG GACGCGTGTG AGACCATGGG CAACGCCACA
    GCCATCTGCT CGGACAAGAC GGGCACGCTG ACCATGAACC GCATGTCTGT GGTGCAGGCT
    TACATTGGAG ACACTCGTTA CCATCAGATC CCAAGCCCTG ATGACCTTGT GCCCAAGGTC
    CTGGACCTTA TTGTCAATGG CATTTCCATC AACAGTGCCT ACACCTCTAA GATTCTGCCT
    CCGGAGAAGG AGGGCGGCCT GCCCCGGCAG GTGGGCAACA AGACCGAGTG CGCCCTGCTG
    GGCTTCGTCT CGGACCTGAA GCAGGATTAC CACGCCGTGC GCAGCGAGGT GCCCGAGGAG
    AAGCTCTACA AAGTGTACAC CTTCAACTCG GTGCGCAAGT CCATGAGCAC GGTCATCGAG
    AAGCCGGGCG GCGGCTACCG CATGTACAGC AAGGGCGCCT CCGAGATCAT CCTGCGCAAG
    TGTAATCGAA TCCTGGACAA AAAGGGGGAG GCCGTGCCAT TCAAGAATAA GGACCGAGAT
    GAGATGGTCC GCACCGTCAT CGAGCCCATG GCCTGTGAGG GGCTGCGGAC ACTCTGCATC
    GCTTACCGGG ATTTCAACGA TGGAGAGCCC CCGTGGGACA ACGAGAGCGA GATCCTCACT
    GAGTTGACCT GTATCGCAGT GGTGGGCATC GAGGACCCCG TGCGCCCGGA GGTGCCAGAA
    GCTATTGCCA AATGCAAGCG AGCTGGCATC ACTGTCAGGA TGGTGACAGG GGACAACATC
    AACACAGCCC GTGCCATCGC CACCAAGTGT GGCATCGTGA CTCCCGGGGA TGACTTTCTG
    TGCTTAGAAG GCAAAGAATT CAATCGCCTC ATCCGAAACG AGAAGGGCGA GGTAGAGCAG
    GAAAAGCTGG ACAAGATCTG GCCTAAGCTC CGAGTGCTGG CGCGGTCTTC CCCCACTGAC
    AAGCACACGC TGGTGAAAGG CATCATCGAC AGCACTGTCG GCGACCAGCG GCAGGTGGTG
    GCCGTCACCG GCGACGGCAC CAACGACGGC CCGGCTCTGA AGAAAGCAGA CGTTGGTTTT
    GCCATGGGTA TTGCAGGCAC AGATGTGGCA AAGGAGGCCT CCGACATCAT CCTAACAGAC
    GACAACTTCA CCAGCATCGT GAAGGCGGTG ATGTGGGGAC GGAACGTCTA CGACAGCATC
    TCCAAGTTCC TGCAGTTCCA GCTCACCGTC AACGTGGTGG CCGTGATCGT GGCCTTCACT
    GGAGCCTGCA TCACCCAGGA TTCCCCACTG AAGGCCGTGC AGATGTTGTG GGTTAATCTA
    ATCATGGACA CCTTTGCCTC GCTGGCCCTG GCCACAGAGC CTCCTACAGA CTCTCTACTG
    AAGCGCCGGC CCTACGGCCG CAATAAGCCT CTAATCTCGC GCACCATGAT GAAGAACATC
    CTGGGTCACG CCGTCTATCA GCTCACCGTC ATCTTTTTCC TTGTGTTTGC GGGTGAGAAA
    TTCTTTGACA TTGACAGCGG GAGGAGAGCG CCTCTGCATT CTCCGCCCAG CCAGCACTAC
    ACCATTATTT TCAACACCTT CGTGCTCATG CAGCTCTTCA ACGAAATCAA CTCCCGCAAG
    ATCCACGGGG AGAGGAATGT CTTCTCGGGC ATCTTCCGCA ATCTCATCTT CTGCTCAGTG
    GTGTTGGGCA CATTCATCAG CCAGATCATC ATCGTGGAAT TCGGGGGCAA ACCCTTCAGT
    TGCACGAAGC TCACCCTGTC CCAGTGGTTC TGGTGCCTCT TCATTGGTAT TGGAGAGCTG
    CTGTGGGGCC AGGTCATTTC CACGATACCT ACCCAATCCC TGAAGTTCCT GAAGGAGGCT
    GGGCATGGCA CCACCAAAGA GGAGATCACC AAGGATGCTG AGGGGCTGGA CGAAATCGAC
    CATGCAGAGA TGGAGCTGCG CCGAGGCCAG ATCCTCTGGT TCCGGGGCCT GAACCGTATC
    CAGACTCAGA TCAAAGTGGT CAAAGCGTTC CATAGTTCCC TCCACGAAAG CATTCAGAAA
    CCCAAGAACC AAAACTCCAT CCACAACTTC ATGACCCACC CTGAATTCAC CATAGATGAG
    GAGGGGCCAC GAACACCACT CCTGGATGAG CAAGAAGAGG AGATTTTTGA AAAGGTGTCT
    AAGCCTGGGA CTAAGACGTC CTCACTGGAT GGTGAGGTCA CTCCACAAAC CAACAAAAAC
    AACAATACGG TGGATTGCTG CCAAGTACAA ATTGTTGCTT CCCACTCGGA CAGCCCTCTA
    CACAGCCTGG AGACGTCCGT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831810.1
    CDS708..4331
    Translation

    Target ORF information:

    RefSeq Version XM_024976042.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ATPase plasma membrane Ca2+ transporting 4 (ATP2B4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976042.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGACGAACC CAACAGAACA CACCTTGCCG TCCAACTCGA TACTTGAGAG CCGTGAAGGG 
    GAGTTTGGCT GCACAGTGAT GGACCTGAGG AAGCTGATGG AGCTGCGCTC AAGTGATGCA
    ATAGACCAGA TCAACGTCCA CTACGGAGGA GTGATGAACC TCTGCAGTAG ACTGAAAACC
    AACCCTGTGG AAGGCCTATC TGGGAACCCT GCAGATCTGG AGAAACGTAA ACAAGTGTTT
    GGACAGAACT TGATTCCCCC CAAAAAGCCC AAGACCTTCT TAGAGTTAGT GTGGGAAGCC
    CTCCAGGATG TCACGCTCAT CATCCTGGAG ATTGCGGCCA TCATCTCCCT GGTCCTGTCC
    TTCTACCGCC CCCCGGGGGG AGAAAATGAA CAGTGTGGTC TAGCCGTCAC TAGCCCAGAG
    GACGAAGGGG AGGCGGAAGC TGGCTGGATT GAGGGTGCAG CCATCTTATT CTCGGTGATC
    ATCGTCGTGC TCGTGACCGC CTTCAATGAC TGGAGCAAGG AGAAGCAATT CCGGGGGCTG
    CAGAACCGCA TCGAAAAGGA GCAGAAATTC TCCGTTATCC GGAATGGCCA CATCATTCAG
    CTCCCTGTGG CTGAGATTGT GGTGGGCGAC ATTGCCCAAA TCAAATACGG TGACCTGCTG
    CCTGCAGATG GGATCCTGAT CCAGGGCAAC GATCTCAAGA TTGATGAGAG TTCTCTGACT
    GGGGAATCAG ACCATGTCAA GAAGTCCTTG GAGAGGGACC CCATGCTGCT CTCAGGGACC
    CATGTAATGG AAGGTTCTGG ACGAATGGTA GTGACGGCTG TGGGTATCAA CTCTCAGACT
    GGAATCATTT TTACCCTCTT GGGAGCCAGT GAGGGGGAAG AGGAAGAGAA GAAGAAGAAA
    GGTAAAAAAC AAGGAGTCCC TGAAAATCGC AACAAAGCAA AGACCCAAGA TGGAGTGGCC
    TTGGAAATCC AGCCACTCAA CAGCCAGGAG GGGATCGACA GTGAGGAAAA GGAGAAGAAG
    GCCGCCAAGC TGCCCAAAAA GGAGAAGTCC GTGCTGCAGG GCAAGCTGAC CCGCCTGGCT
    GTCCAGATTG GGAAAGCTGG TCTGATCATG TCTGCAATCA CAGTTCTCAT CCTGATTCTG
    TACTTCGTGA TCGACAACTT TGTGATCCAG CGCAGACCGT GGCTGGCCGA GTGTACCCCC
    ATCTACGTCC AGTACTTTGT CAAGTTCTTC ATCATCGGCG TCACGGTGCT GGTGGTGGCT
    GTGCCCGAGG GGCTGCCCCT GGCCGTCACC ATCTCACTGG CTTACTCTGT GAAGAAAATG
    ATGAAGGACA ACAACCTGGT GCGGCACTTG GACGCGTGTG AGACCATGGG CAACGCCACA
    GCCATCTGCT CGGACAAGAC GGGCACGCTG ACCATGAACC GCATGTCTGT GGTGCAGGCT
    TACATTGGAG ACACTCGTTA CCATCAGATC CCAAGCCCTG ATGACCTTGT GCCCAAGGTC
    CTGGACCTTA TTGTCAATGG CATTTCCATC AACAGTGCCT ACACCTCTAA GATTCTGCCT
    CCGGAGAAGG AGGGCGGCCT GCCCCGGCAG GTGGGCAACA AGACCGAGTG CGCCCTGCTG
    GGCTTCGTCT CGGACCTGAA GCAGGATTAC CACGCCGTGC GCAGCGAGGT GCCCGAGGAG
    AAGCTCTACA AAGTGTACAC CTTCAACTCG GTGCGCAAGT CCATGAGCAC GGTCATCGAG
    AAGCCGGGCG GCGGCTACCG CATGTACAGC AAGGGCGCCT CCGAGATCAT CCTGCGCAAG
    TGTAATCGAA TCCTGGACAA AAAGGGGGAG GCCGTGCCAT TCAAGAATAA GGACCGAGAT
    GAGATGGTCC GCACCGTCAT CGAGCCCATG GCCTGTGAGG GGCTGCGGAC ACTCTGCATC
    GCTTACCGGG ATTTCAACGA TGGAGAGCCC CCGTGGGACA ACGAGAGCGA GATCCTCACT
    GAGTTGACCT GTATCGCAGT GGTGGGCATC GAGGACCCCG TGCGCCCGGA GGTGCCAGAA
    GCTATTGCCA AATGCAAGCG AGCTGGCATC ACTGTCAGGA TGGTGACAGG GGACAACATC
    AACACAGCCC GTGCCATCGC CACCAAGTGT GGCATCGTGA CTCCCGGGGA TGACTTTCTG
    TGCTTAGAAG GCAAAGAATT CAATCGCCTC ATCCGAAACG AGAAGGGCGA GGTAGAGCAG
    GAAAAGCTGG ACAAGATCTG GCCTAAGCTC CGAGTGCTGG CGCGGTCTTC CCCCACTGAC
    AAGCACACGC TGGTGAAAGG CATCATCGAC AGCACTGTCG GCGACCAGCG GCAGGTGGTG
    GCCGTCACCG GCGACGGCAC CAACGACGGC CCGGCTCTGA AGAAAGCAGA CGTTGGTTTT
    GCCATGGGTA TTGCAGGCAC AGATGTGGCA AAGGAGGCCT CCGACATCAT CCTAACAGAC
    GACAACTTCA CCAGCATCGT GAAGGCGGTG ATGTGGGGAC GGAACGTCTA CGACAGCATC
    TCCAAGTTCC TGCAGTTCCA GCTCACCGTC AACGTGGTGG CCGTGATCGT GGCCTTCACT
    GGAGCCTGCA TCACCCAGGA TTCCCCACTG AAGGCCGTGC AGATGTTGTG GGTTAATCTA
    ATCATGGACA CCTTTGCCTC GCTGGCCCTG GCCACAGAGC CTCCTACAGA CTCTCTACTG
    AAGCGCCGGC CCTACGGCCG CAATAAGCCT CTAATCTCGC GCACCATGAT GAAGAACATC
    CTGGGTCACG CCGTCTATCA GCTCACCGTC ATCTTTTTCC TTGTGTTTGC GGGTGAGAAA
    TTCTTTGACA TTGACAGCGG GAGGAGAGCG CCTCTGCATT CTCCGCCCAG CCAGCACTAC
    ACCATTATTT TCAACACCTT CGTGCTCATG CAGCTCTTCA ACGAAATCAA CTCCCGCAAG
    ATCCACGGGG AGAGGAATGT CTTCTCGGGC ATCTTCCGCA ATCTCATCTT CTGCTCAGTG
    GTGTTGGGCA CATTCATCAG CCAGATCATC ATCGTGGAAT TCGGGGGCAA ACCCTTCAGT
    TGCACGAAGC TCACCCTGTC CCAGTGGTTC TGGTGCCTCT TCATTGGTAT TGGAGAGCTG
    CTGTGGGGCC AGGTCATTTC CACGATACCT ACCCAATCCC TGAAGTTCCT GAAGGAGGCT
    GGGCATGGCA CCACCAAAGA GGAGATCACC AAGGATGCTG AGGGGCTGGA CGAAATCGAC
    CATGCAGAGA TGGAGCTGCG CCGAGGCCAG ATCCTCTGGT TCCGGGGCCT GAACCGTATC
    CAGACTCAGA TCAAAGTGGT CAAAGCGTTC CATAGTTCCC TCCACGAAAG CATTCAGAAA
    CCCAAGAACC AAAACTCCAT CCACAACTTC ATGACCCACC CTGAATTCAC CATAGATGAG
    GAGGGGCCAC GAACACCACT CCTGGATGAG CAAGAAGAGG AGATTTTTGA AAAGGTGTCT
    AAGCCTGGGA CTAAGACGTC CTCACTGGAT GGTGAGGTCA CTCCACAAAC CAACAAAAAC
    AACAATACGG TGGATTGCTG CCAAGTACAA ATTGTTGCTT CCCACTCGGA CAGCCCTCTA
    CACAGCCTGG AGACGTCCGT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223429
    Clone ID Related Accession (Same CDS sequence) XM_024976046.1
    Accession Version XM_024976046.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3588bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product plasma membrane calcium-transporting ATPase 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGACGAACC CAACAGAACA CACCTTGCCG TCCAACTCGA TACTTGAGAG CCGTGAAGGG 
    GAGTTTGGCT GCACAGTGAT GGACCTGAGG AAGCTGATGG AGCTGCGCTC AAGTGATGCA
    ATAGACCAGA TCAACGTCCA CTACGGAGGA GTGATGAACC TCTGCAGTAG ACTGAAAACC
    AACCCTGTGG AAGGCCTATC TGGGAACCCT GCAGATCTGG AGAAACGTAA ACAAGTGTTT
    GGACAGAACT TGATTCCCCC CAAAAAGCCC AAGACCTTCT TAGAGTTAGT GTGGGAAGCC
    CTCCAGGATG TCACGCTCAT CATCCTGGAG ATTGCGGCCA TCATCTCCCT GGTCCTGTCC
    TTCTACCGCC CCCCGGGGGG AGAAAATGAA CAGTGTGGTC TAGCCGTCAC TAGCCCAGAG
    GACGAAGGGG AGGCGGAAGC TGGCTGGATT GAGGGTGCAG CCATCTTATT CTCGGTGATC
    ATCGTCGTGC TCGTGACCGC CTTCAATGAC TGGAGCAAGG AGAAGCAATT CCGGGGGCTG
    CAGAACCGCA TCGAAAAGGA GCAGAAATTC TCCGTTATCC GGAATGGCCA CATCATTCAG
    CTCCCTGTGG CTGAGATTGT GGTGGGCGAC ATTGCCCAAA TCAAATACGG TGACCTGCTG
    CCTGCAGATG GGATCCTGAT CCAGGGCAAC GATCTCAAGA TTGATGAGAG TTCTCTGACT
    GGGGAATCAG ACCATGTCAA GAAGTCCTTG GAGAGGGACC CCATGCTGCT CTCAGGGACC
    CATGTAATGG AAGGTTCTGG ACGAATGGTA GTGACGGCTG TGGGTATCAA CTCTCAGACT
    GGAATCATTT TTACCCTCTT GGGAGCCAGT GAGGGGGAAG AGGAAGAGAA GAAGAAGAAA
    GCAAAGACCC AAGATGGAGT GGCCTTGGAA ATCCAGCCAC TCAACAGCCA GGAGGGGATC
    GACAGTGAGG AAAAGGAGAA GAAGGCCGCC AAGCTGCCCA AAAAGGAGAA GTCCGTGCTG
    CAGGGCAAGC TGACCCGCCT GGCTGTCCAG ATTGGGAAAG CTGGTCTGAT CATGTCTGCA
    ATCACAGTTC TCATCCTGAT TCTGTACTTC GTGATCGACA ACTTTGTGAT CCAGCGCAGA
    CCGTGGCTGG CCGAGTGTAC CCCCATCTAC GTCCAGTACT TTGTCAAGTT CTTCATCATC
    GGCGTCACGG TGCTGGTGGT GGCTGTGCCC GAGGGGCTGC CCCTGGCCGT CACCATCTCA
    CTGGCTTACT CTGTGAAGAA AATGATGAAG GACAACAACC TGGTGCGGCA CTTGGACGCG
    TGTGAGACCA TGGGCAACGC CACAGCCATC TGCTCGGACA AGACGGGCAC GCTGACCATG
    AACCGCATGT CTGTGGTGCA GGCTTACATT GGAGACACTC GTTACCATCA GATCCCAAGC
    CCTGATGACC TTGTGCCCAA GGTCCTGGAC CTTATTGTCA ATGGCATTTC CATCAACAGT
    GCCTACACCT CTAAGATTCT GCCTCCGGAG AAGGAGGGCG GCCTGCCCCG GCAGGTGGGC
    AACAAGACCG AGTGCGCCCT GCTGGGCTTC GTCTCGGACC TGAAGCAGGA TTACCACGCC
    GTGCGCAGCG AGGTGCCCGA GGAGAAGCTC TACAAAGTGT ACACCTTCAA CTCGGTGCGC
    AAGTCCATGA GCACGGTCAT CGAGAAGCCG GGCGGCGGCT ACCGCATGTA CAGCAAGGGC
    GCCTCCGAGA TCATCCTGCG CAAGTGTAAT CGAATCCTGG ACAAAAAGGG GGAGGCCGTG
    CCATTCAAGA ATAAGGACCG AGATGAGATG GTCCGCACCG TCATCGAGCC CATGGCCTGT
    GAGGGGCTGC GGACACTCTG CATCGCTTAC CGGGATTTCA ACGATGGAGA GCCCCCGTGG
    GACAACGAGA GCGAGATCCT CACTGAGTTG ACCTGTATCG CAGTGGTGGG CATCGAGGAC
    CCCGTGCGCC CGGAGGTGCC AGAAGCTATT GCCAAATGCA AGCGAGCTGG CATCACTGTC
    AGGATGGTGA CAGGGGACAA CATCAACACA GCCCGTGCCA TCGCCACCAA GTGTGGCATC
    GTGACTCCCG GGGATGACTT TCTGTGCTTA GAAGGCAAAG AATTCAATCG CCTCATCCGA
    AACGAGAAGG GCGAGGTAGA GCAGGAAAAG CTGGACAAGA TCTGGCCTAA GCTCCGAGTG
    CTGGCGCGGT CTTCCCCCAC TGACAAGCAC ACGCTGGTGA AAGGCATCAT CGACAGCACT
    GTCGGCGACC AGCGGCAGGT GGTGGCCGTC ACCGGCGACG GCACCAACGA CGGCCCGGCT
    CTGAAGAAAG CAGACGTTGG TTTTGCCATG GGTATTGCAG GCACAGATGT GGCAAAGGAG
    GCCTCCGACA TCATCCTAAC AGACGACAAC TTCACCAGCA TCGTGAAGGC GGTGATGTGG
    GGACGGAACG TCTACGACAG CATCTCCAAG TTCCTGCAGT TCCAGCTCAC CGTCAACGTG
    GTGGCCGTGA TCGTGGCCTT CACTGGAGCC TGCATCACCC AGGATTCCCC ACTGAAGGCC
    GTGCAGATGT TGTGGGTTAA TCTAATCATG GACACCTTTG CCTCGCTGGC CCTGGCCACA
    GAGCCTCCTA CAGACTCTCT ACTGAAGCGC CGGCCCTACG GCCGCAATAA GCCTCTAATC
    TCGCGCACCA TGATGAAGAA CATCCTGGGT CACGCCGTCT ATCAGCTCAC CGTCATCTTT
    TTCCTTGTGT TTGCGGGTGA GAAATTCTTT GACATTGACA GCGGGAGGAG AGCGCCTCTG
    CATTCTCCGC CCAGCCAGCA CTACACCATT ATTTTCAACA CCTTCGTGCT CATGCAGCTC
    TTCAACGAAA TCAACTCCCG CAAGATCCAC GGGGAGAGGA ATGTCTTCTC GGGCATCTTC
    CGCAATCTCA TCTTCTGCTC AGTGGTGTTG GGCACATTCA TCAGCCAGAT CATCATCGTG
    GAATTCGGGG GCAAACCCTT CAGTTGCACG AAGCTCACCC TGTCCCAGTG GTTCTGGTGC
    CTCTTCATTG GTATTGGAGA GCTGCTGTGG GGCCAGGTCA TTTCCACGAT ACCTACCCAA
    TCCCTGAAGT TCCTGAAGGA GGCTGGGCAT GGCACCACCA AAGAGGAGAT CACCAAGGAT
    GCTGAGGGGC TGGACGAAAT CGACCATGCA GAGATGGAGC TGCGCCGAGG CCAGATCCTC
    TGGTTCCGGG GCCTGAACCG TATCCAGACT CAGATCAAAG TGGTCAAAGC GTTCCATAGT
    TCCCTCCACG AAAGCATTCA GAAACCCAAG AACCAAAACT CCATCCACAA CTTCATGACC
    CACCCTGAAT TCACCATAGA TGAGGAGGGG CCACGAACAC CACTCCTGGA TGAGCAAGAA
    GAGGAGATTT TTGAAAAGGT GTCTAAGCCT GGGACTAAGA CGTCCTCACT GGATGGTGAG
    GTCACTCCAC AAACCAACAA AAACAACAAT ACGGTGGATT GCTGCCAAGT ACAAATTGTT
    GCTTCCCACT CGGACAGCCC TCTACACAGC CTGGAGACGT CCGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831814.1
    CDS933..4520
    Translation

    Target ORF information:

    RefSeq Version XM_024976046.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ATPase plasma membrane Ca2+ transporting 4 (ATP2B4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976046.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGACGAACC CAACAGAACA CACCTTGCCG TCCAACTCGA TACTTGAGAG CCGTGAAGGG 
    GAGTTTGGCT GCACAGTGAT GGACCTGAGG AAGCTGATGG AGCTGCGCTC AAGTGATGCA
    ATAGACCAGA TCAACGTCCA CTACGGAGGA GTGATGAACC TCTGCAGTAG ACTGAAAACC
    AACCCTGTGG AAGGCCTATC TGGGAACCCT GCAGATCTGG AGAAACGTAA ACAAGTGTTT
    GGACAGAACT TGATTCCCCC CAAAAAGCCC AAGACCTTCT TAGAGTTAGT GTGGGAAGCC
    CTCCAGGATG TCACGCTCAT CATCCTGGAG ATTGCGGCCA TCATCTCCCT GGTCCTGTCC
    TTCTACCGCC CCCCGGGGGG AGAAAATGAA CAGTGTGGTC TAGCCGTCAC TAGCCCAGAG
    GACGAAGGGG AGGCGGAAGC TGGCTGGATT GAGGGTGCAG CCATCTTATT CTCGGTGATC
    ATCGTCGTGC TCGTGACCGC CTTCAATGAC TGGAGCAAGG AGAAGCAATT CCGGGGGCTG
    CAGAACCGCA TCGAAAAGGA GCAGAAATTC TCCGTTATCC GGAATGGCCA CATCATTCAG
    CTCCCTGTGG CTGAGATTGT GGTGGGCGAC ATTGCCCAAA TCAAATACGG TGACCTGCTG
    CCTGCAGATG GGATCCTGAT CCAGGGCAAC GATCTCAAGA TTGATGAGAG TTCTCTGACT
    GGGGAATCAG ACCATGTCAA GAAGTCCTTG GAGAGGGACC CCATGCTGCT CTCAGGGACC
    CATGTAATGG AAGGTTCTGG ACGAATGGTA GTGACGGCTG TGGGTATCAA CTCTCAGACT
    GGAATCATTT TTACCCTCTT GGGAGCCAGT GAGGGGGAAG AGGAAGAGAA GAAGAAGAAA
    GCAAAGACCC AAGATGGAGT GGCCTTGGAA ATCCAGCCAC TCAACAGCCA GGAGGGGATC
    GACAGTGAGG AAAAGGAGAA GAAGGCCGCC AAGCTGCCCA AAAAGGAGAA GTCCGTGCTG
    CAGGGCAAGC TGACCCGCCT GGCTGTCCAG ATTGGGAAAG CTGGTCTGAT CATGTCTGCA
    ATCACAGTTC TCATCCTGAT TCTGTACTTC GTGATCGACA ACTTTGTGAT CCAGCGCAGA
    CCGTGGCTGG CCGAGTGTAC CCCCATCTAC GTCCAGTACT TTGTCAAGTT CTTCATCATC
    GGCGTCACGG TGCTGGTGGT GGCTGTGCCC GAGGGGCTGC CCCTGGCCGT CACCATCTCA
    CTGGCTTACT CTGTGAAGAA AATGATGAAG GACAACAACC TGGTGCGGCA CTTGGACGCG
    TGTGAGACCA TGGGCAACGC CACAGCCATC TGCTCGGACA AGACGGGCAC GCTGACCATG
    AACCGCATGT CTGTGGTGCA GGCTTACATT GGAGACACTC GTTACCATCA GATCCCAAGC
    CCTGATGACC TTGTGCCCAA GGTCCTGGAC CTTATTGTCA ATGGCATTTC CATCAACAGT
    GCCTACACCT CTAAGATTCT GCCTCCGGAG AAGGAGGGCG GCCTGCCCCG GCAGGTGGGC
    AACAAGACCG AGTGCGCCCT GCTGGGCTTC GTCTCGGACC TGAAGCAGGA TTACCACGCC
    GTGCGCAGCG AGGTGCCCGA GGAGAAGCTC TACAAAGTGT ACACCTTCAA CTCGGTGCGC
    AAGTCCATGA GCACGGTCAT CGAGAAGCCG GGCGGCGGCT ACCGCATGTA CAGCAAGGGC
    GCCTCCGAGA TCATCCTGCG CAAGTGTAAT CGAATCCTGG ACAAAAAGGG GGAGGCCGTG
    CCATTCAAGA ATAAGGACCG AGATGAGATG GTCCGCACCG TCATCGAGCC CATGGCCTGT
    GAGGGGCTGC GGACACTCTG CATCGCTTAC CGGGATTTCA ACGATGGAGA GCCCCCGTGG
    GACAACGAGA GCGAGATCCT CACTGAGTTG ACCTGTATCG CAGTGGTGGG CATCGAGGAC
    CCCGTGCGCC CGGAGGTGCC AGAAGCTATT GCCAAATGCA AGCGAGCTGG CATCACTGTC
    AGGATGGTGA CAGGGGACAA CATCAACACA GCCCGTGCCA TCGCCACCAA GTGTGGCATC
    GTGACTCCCG GGGATGACTT TCTGTGCTTA GAAGGCAAAG AATTCAATCG CCTCATCCGA
    AACGAGAAGG GCGAGGTAGA GCAGGAAAAG CTGGACAAGA TCTGGCCTAA GCTCCGAGTG
    CTGGCGCGGT CTTCCCCCAC TGACAAGCAC ACGCTGGTGA AAGGCATCAT CGACAGCACT
    GTCGGCGACC AGCGGCAGGT GGTGGCCGTC ACCGGCGACG GCACCAACGA CGGCCCGGCT
    CTGAAGAAAG CAGACGTTGG TTTTGCCATG GGTATTGCAG GCACAGATGT GGCAAAGGAG
    GCCTCCGACA TCATCCTAAC AGACGACAAC TTCACCAGCA TCGTGAAGGC GGTGATGTGG
    GGACGGAACG TCTACGACAG CATCTCCAAG TTCCTGCAGT TCCAGCTCAC CGTCAACGTG
    GTGGCCGTGA TCGTGGCCTT CACTGGAGCC TGCATCACCC AGGATTCCCC ACTGAAGGCC
    GTGCAGATGT TGTGGGTTAA TCTAATCATG GACACCTTTG CCTCGCTGGC CCTGGCCACA
    GAGCCTCCTA CAGACTCTCT ACTGAAGCGC CGGCCCTACG GCCGCAATAA GCCTCTAATC
    TCGCGCACCA TGATGAAGAA CATCCTGGGT CACGCCGTCT ATCAGCTCAC CGTCATCTTT
    TTCCTTGTGT TTGCGGGTGA GAAATTCTTT GACATTGACA GCGGGAGGAG AGCGCCTCTG
    CATTCTCCGC CCAGCCAGCA CTACACCATT ATTTTCAACA CCTTCGTGCT CATGCAGCTC
    TTCAACGAAA TCAACTCCCG CAAGATCCAC GGGGAGAGGA ATGTCTTCTC GGGCATCTTC
    CGCAATCTCA TCTTCTGCTC AGTGGTGTTG GGCACATTCA TCAGCCAGAT CATCATCGTG
    GAATTCGGGG GCAAACCCTT CAGTTGCACG AAGCTCACCC TGTCCCAGTG GTTCTGGTGC
    CTCTTCATTG GTATTGGAGA GCTGCTGTGG GGCCAGGTCA TTTCCACGAT ACCTACCCAA
    TCCCTGAAGT TCCTGAAGGA GGCTGGGCAT GGCACCACCA AAGAGGAGAT CACCAAGGAT
    GCTGAGGGGC TGGACGAAAT CGACCATGCA GAGATGGAGC TGCGCCGAGG CCAGATCCTC
    TGGTTCCGGG GCCTGAACCG TATCCAGACT CAGATCAAAG TGGTCAAAGC GTTCCATAGT
    TCCCTCCACG AAAGCATTCA GAAACCCAAG AACCAAAACT CCATCCACAA CTTCATGACC
    CACCCTGAAT TCACCATAGA TGAGGAGGGG CCACGAACAC CACTCCTGGA TGAGCAAGAA
    GAGGAGATTT TTGAAAAGGT GTCTAAGCCT GGGACTAAGA CGTCCTCACT GGATGGTGAG
    GTCACTCCAC AAACCAACAA AAACAACAAT ACGGTGGATT GCTGCCAAGT ACAAATTGTT
    GCTTCCCACT CGGACAGCCC TCTACACAGC CTGGAGACGT CCGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223430
    Clone ID Related Accession (Same CDS sequence) XM_024976047.1
    Accession Version XM_024976047.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3516bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product plasma membrane calcium-transporting ATPase 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGACGAACC CAACAGAACA CACCTTGCCG TCCAACTCGA TACTTGAGAG CCGTGAAGGG 
    GAGTTTGGCT GCACAGTGAT GGACCTGAGG AAGCTGATGG AGCTGCGCTC AAGTGATGCA
    ATAGACCAGA TCAACGTCCA CTACGGAGGA GTGATGAACC TCTGCAGTAG ACTGAAAACC
    AACCCTGTGG AAGGCCTATC TGGGAACCCT GCAGATCTGG AGAAACGTAA ACAAGTGTTT
    GGACAGAACT TGATTCCCCC CAAAAAGCCC AAGACCTTCT TAGAGTTAGT GTGGGAAGCC
    CTCCAGGATG TCACGCTCAT CATCCTGGAG ATTGCGGCCA TCATCTCCCT GGTCCTGTCC
    TTCTACCGCC CCCCGGGGGG AGAAAATGAA CAGTGTGGTC TAGCCGTCAC TAGCCCAGAG
    GACGAAGGGG AGGCGGAAGC TGGCTGGATT GAGGGTGCAG CCATCTTATT CTCGGTGATC
    ATCGTCGTGC TCGTGACCGC CTTCAATGAC TGGAGCAAGG AGAAGCAATT CCGGGGGCTG
    CAGAACCGCA TCGAAAAGGA GCAGAAATTC TCCGTTATCC GGAATGGCCA CATCATTCAG
    CTCCCTGTGG CTGAGATTGT GGTGGGCGAC ATTGCCCAAA TCAAATACGG TGACCTGCTG
    CCTGCAGATG GGATCCTGAT CCAGGGCAAC GATCTCAAGA TTGATGAGAG TTCTCTGACT
    GGGGAATCAG ACCATGTCAA GAAGTCCTTG GAGAGGGACC CCATGCTGCT CTCAGGGACC
    CATGTAATGG AAGGTTCTGG ACGAATGGTA GTGACGGCTG TGGGTATCAA CTCTCAGACT
    GGAATCATTT TTACCCTCTT GGGAGCCAGT GAGGGGGAAG AGGAAGAGAA GAAGAAGAAA
    GGTAAAAAAC AAGGAGTCCC TGAAAATCGC AACAAAGCAA AGACCCAAGA TGGAGTGGCC
    TTGGAAATCC AGCCACTCAA CAGCCAGGAG GGGATCGACA GTGAGGAAAA GGAGAAGAAG
    GCCGCCAAGC TGCCCAAAAA GGAGAAGTCC GTGCTGCAGG GCAAGCTGAC CCGCCTGGCT
    GTCCAGATTG GGAAAGCTGG TCTGATCATG TCTGCAATCA CAGTTCTCAT CCTGATTCTG
    TACTTCGTGA TCGACAACTT TGTGATCCAG CGCAGACCGT GGCTGGCCGA GTGTACCCCC
    ATCTACGTCC AGTACTTTGT CAAGTTCTTC ATCATCGGCG TCACGGTGCT GGTGGTGGCT
    GTGCCCGAGG GGCTGCCCCT GGCCGTCACC ATCTCACTGG CTTACTCTGT GAAGAAAATG
    ATGAAGGACA ACAACCTGGT GCGGCACTTG GACGCGTGTG AGACCATGGG CAACGCCACA
    GCCATCTGCT CGGACAAGAC GGGCACGCTG ACCATGAACC GCATGTCTGT GGTGCAGGCT
    TACATTGGAG ACACTCGTTA CCATCAGATC CCAAGCCCTG ATGACCTTGT GCCCAAGGTC
    CTGGACCTTA TTGTCAATGG CATTTCCATC AACAGTGCCT ACACCTCTAA GATTCTGCCT
    CCGGAGAAGG AGGGCGGCCT GCCCCGGCAG GTGGGCAACA AGACCGAGTG CGCCCTGCTG
    GGCTTCGTCT CGGACCTGAA GCAGGATTAC CACGCCGTGC GCAGCGAGGT GCCCGAGGAG
    AAGCTCTACA AAGTGTACAC CTTCAACTCG GTGCGCAAGT CCATGAGCAC GGTCATCGAG
    AAGCCGGGCG GCGGCTACCG CATGTACAGC AAGGGCGCCT CCGAGATCAT CCTGCGCAAG
    TGTAATCGAA TCCTGGACAA AAAGGGGGAG GCCGTGCCAT TCAAGAATAA GGACCGAGAT
    GAGATGGTCC GCACCGTCAT CGAGCCCATG GCCTGTGAGG GGCTGCGGAC ACTCTGCATC
    GCTTACCGGG ATTTCAACGA TGGAGAGCCC CCGTGGGACA ACGAGAGCGA GATCCTCACT
    GAGTTGACCT GTATCGCAGT GGTGGGCATC GAGGACCCCG TGCGCCCGGA GGTGCCAGAA
    GCTATTGCCA AATGCAAGCG AGCTGGCATC ACTGTCAGGA TGGTGACAGG GGACAACATC
    AACACAGCCC GTGCCATCGC CACCAAGTGT GGCATCGTGA CTCCCGGGGA TGACTTTCTG
    TGCTTAGAAG GCAAAGAATT CAATCGCCTC ATCCGAAACG AGAAGGGCGA GGTAGAGCAG
    GAAAAGCTGG ACAAGATCTG GCCTAAGCTC CGAGTGCTGG CGCGGTCTTC CCCCACTGAC
    AAGCACACGC TGGTGAAAGG CATCATCGAC AGCACTGTCG GCGACCAGCG GCAGGTGGTG
    GCCGTCACCG GCGACGGCAC CAACGACGGC CCGGCTCTGA AGAAAGCAGA CGTTGGTTTT
    GCCATGGGTA TTGCAGGCAC AGATGTGGCA AAGGAGGCCT CCGACATCAT CCTAACAGAC
    GACAACTTCA CCAGCATCGT GAAGGCGGTG ATGTGGGGAC GGAACGTCTA CGACAGCATC
    TCCAAGTTCC TGCAGTTCCA GCTCACCGTC AACGTGGTGG CCGTGATCGT GGCCTTCACT
    GGAGCCTGCA TCACCCAGGA TTCCCCACTG AAGGCCGTGC AGATGTTGTG GGTTAATCTA
    ATCATGGACA CCTTTGCCTC GCTGGCCCTG GCCACAGAGC CTCCTACAGA CTCTCTACTG
    AAGCGCCGGC CCTACGGCCG CAATAAGCCT CTAATCTCGC GCACCATGAT GAAGAACATC
    CTGGGTCACG CCGTCTATCA GCTCACCGTC ATCTTTTTCC TTGTGTTTGC GGGTGAGAAA
    TTCTTTGACA TTGACAGCGG GAGGAGAGCG CCTCTGCATT CTCCGCCCAG CCAGCACTAC
    ACCATTATTT TCAACACCTT CGTGCTCATG CAGCTCTTCA ACGAAATCAA CTCCCGCAAG
    ATCCACGGGG AGAGGAATGT CTTCTCGGGC ATCTTCCGCA ATCTCATCTT CTGCTCAGTG
    GTGTTGGGCA CATTCATCAG CCAGATCATC ATCGTGGAAT TCGGGGGCAA ACCCTTCAGT
    TGCACGAAGC TCACCCTGTC CCAGTGGTTC TGGTGCCTCT TCATTGGTAT TGGAGAGCTG
    CTGTGGGGCC AGGTCATTTC CACGATACCT ACCCAATCCC TGAAGTTCCT GAAGGAGGCT
    GGGCATGGCA CCACCAAAGA GGAGATCACC AAGGATGCTG AGGGGCTGGA CGAAATCGAC
    CATGCAGAGA TGGAGCTGCG CCGAGGCCAG ATCCTCTGGT TCCGGGGCCT GAACCGTATC
    CAGACTCAGA TCGACGTAAT TAACACATTC CAGACGGGAG CCTCTTTTAA GGGAGTCCTA
    AAGCGCCAGA CCATGGGTCA ACACCTTGAT GTAAAACATG TTCCTAGCTC ATCCTATGTA
    ACAGTTGCAC CAGTCAAATC TCCCCCCACC ACTTCTGTTG CTGCTGCTGT TTCATCTCCT
    ACTCTGGGCA ATCAAAGTGG TCAAAGCGTT CCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831815.1
    CDS939..4454
    Translation

    Target ORF information:

    RefSeq Version XM_024976047.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ATPase plasma membrane Ca2+ transporting 4 (ATP2B4), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976047.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGACGAACC CAACAGAACA CACCTTGCCG TCCAACTCGA TACTTGAGAG CCGTGAAGGG 
    GAGTTTGGCT GCACAGTGAT GGACCTGAGG AAGCTGATGG AGCTGCGCTC AAGTGATGCA
    ATAGACCAGA TCAACGTCCA CTACGGAGGA GTGATGAACC TCTGCAGTAG ACTGAAAACC
    AACCCTGTGG AAGGCCTATC TGGGAACCCT GCAGATCTGG AGAAACGTAA ACAAGTGTTT
    GGACAGAACT TGATTCCCCC CAAAAAGCCC AAGACCTTCT TAGAGTTAGT GTGGGAAGCC
    CTCCAGGATG TCACGCTCAT CATCCTGGAG ATTGCGGCCA TCATCTCCCT GGTCCTGTCC
    TTCTACCGCC CCCCGGGGGG AGAAAATGAA CAGTGTGGTC TAGCCGTCAC TAGCCCAGAG
    GACGAAGGGG AGGCGGAAGC TGGCTGGATT GAGGGTGCAG CCATCTTATT CTCGGTGATC
    ATCGTCGTGC TCGTGACCGC CTTCAATGAC TGGAGCAAGG AGAAGCAATT CCGGGGGCTG
    CAGAACCGCA TCGAAAAGGA GCAGAAATTC TCCGTTATCC GGAATGGCCA CATCATTCAG
    CTCCCTGTGG CTGAGATTGT GGTGGGCGAC ATTGCCCAAA TCAAATACGG TGACCTGCTG
    CCTGCAGATG GGATCCTGAT CCAGGGCAAC GATCTCAAGA TTGATGAGAG TTCTCTGACT
    GGGGAATCAG ACCATGTCAA GAAGTCCTTG GAGAGGGACC CCATGCTGCT CTCAGGGACC
    CATGTAATGG AAGGTTCTGG ACGAATGGTA GTGACGGCTG TGGGTATCAA CTCTCAGACT
    GGAATCATTT TTACCCTCTT GGGAGCCAGT GAGGGGGAAG AGGAAGAGAA GAAGAAGAAA
    GGTAAAAAAC AAGGAGTCCC TGAAAATCGC AACAAAGCAA AGACCCAAGA TGGAGTGGCC
    TTGGAAATCC AGCCACTCAA CAGCCAGGAG GGGATCGACA GTGAGGAAAA GGAGAAGAAG
    GCCGCCAAGC TGCCCAAAAA GGAGAAGTCC GTGCTGCAGG GCAAGCTGAC CCGCCTGGCT
    GTCCAGATTG GGAAAGCTGG TCTGATCATG TCTGCAATCA CAGTTCTCAT CCTGATTCTG
    TACTTCGTGA TCGACAACTT TGTGATCCAG CGCAGACCGT GGCTGGCCGA GTGTACCCCC
    ATCTACGTCC AGTACTTTGT CAAGTTCTTC ATCATCGGCG TCACGGTGCT GGTGGTGGCT
    GTGCCCGAGG GGCTGCCCCT GGCCGTCACC ATCTCACTGG CTTACTCTGT GAAGAAAATG
    ATGAAGGACA ACAACCTGGT GCGGCACTTG GACGCGTGTG AGACCATGGG CAACGCCACA
    GCCATCTGCT CGGACAAGAC GGGCACGCTG ACCATGAACC GCATGTCTGT GGTGCAGGCT
    TACATTGGAG ACACTCGTTA CCATCAGATC CCAAGCCCTG ATGACCTTGT GCCCAAGGTC
    CTGGACCTTA TTGTCAATGG CATTTCCATC AACAGTGCCT ACACCTCTAA GATTCTGCCT
    CCGGAGAAGG AGGGCGGCCT GCCCCGGCAG GTGGGCAACA AGACCGAGTG CGCCCTGCTG
    GGCTTCGTCT CGGACCTGAA GCAGGATTAC CACGCCGTGC GCAGCGAGGT GCCCGAGGAG
    AAGCTCTACA AAGTGTACAC CTTCAACTCG GTGCGCAAGT CCATGAGCAC GGTCATCGAG
    AAGCCGGGCG GCGGCTACCG CATGTACAGC AAGGGCGCCT CCGAGATCAT CCTGCGCAAG
    TGTAATCGAA TCCTGGACAA AAAGGGGGAG GCCGTGCCAT TCAAGAATAA GGACCGAGAT
    GAGATGGTCC GCACCGTCAT CGAGCCCATG GCCTGTGAGG GGCTGCGGAC ACTCTGCATC
    GCTTACCGGG ATTTCAACGA TGGAGAGCCC CCGTGGGACA ACGAGAGCGA GATCCTCACT
    GAGTTGACCT GTATCGCAGT GGTGGGCATC GAGGACCCCG TGCGCCCGGA GGTGCCAGAA
    GCTATTGCCA AATGCAAGCG AGCTGGCATC ACTGTCAGGA TGGTGACAGG GGACAACATC
    AACACAGCCC GTGCCATCGC CACCAAGTGT GGCATCGTGA CTCCCGGGGA TGACTTTCTG
    TGCTTAGAAG GCAAAGAATT CAATCGCCTC ATCCGAAACG AGAAGGGCGA GGTAGAGCAG
    GAAAAGCTGG ACAAGATCTG GCCTAAGCTC CGAGTGCTGG CGCGGTCTTC CCCCACTGAC
    AAGCACACGC TGGTGAAAGG CATCATCGAC AGCACTGTCG GCGACCAGCG GCAGGTGGTG
    GCCGTCACCG GCGACGGCAC CAACGACGGC CCGGCTCTGA AGAAAGCAGA CGTTGGTTTT
    GCCATGGGTA TTGCAGGCAC AGATGTGGCA AAGGAGGCCT CCGACATCAT CCTAACAGAC
    GACAACTTCA CCAGCATCGT GAAGGCGGTG ATGTGGGGAC GGAACGTCTA CGACAGCATC
    TCCAAGTTCC TGCAGTTCCA GCTCACCGTC AACGTGGTGG CCGTGATCGT GGCCTTCACT
    GGAGCCTGCA TCACCCAGGA TTCCCCACTG AAGGCCGTGC AGATGTTGTG GGTTAATCTA
    ATCATGGACA CCTTTGCCTC GCTGGCCCTG GCCACAGAGC CTCCTACAGA CTCTCTACTG
    AAGCGCCGGC CCTACGGCCG CAATAAGCCT CTAATCTCGC GCACCATGAT GAAGAACATC
    CTGGGTCACG CCGTCTATCA GCTCACCGTC ATCTTTTTCC TTGTGTTTGC GGGTGAGAAA
    TTCTTTGACA TTGACAGCGG GAGGAGAGCG CCTCTGCATT CTCCGCCCAG CCAGCACTAC
    ACCATTATTT TCAACACCTT CGTGCTCATG CAGCTCTTCA ACGAAATCAA CTCCCGCAAG
    ATCCACGGGG AGAGGAATGT CTTCTCGGGC ATCTTCCGCA ATCTCATCTT CTGCTCAGTG
    GTGTTGGGCA CATTCATCAG CCAGATCATC ATCGTGGAAT TCGGGGGCAA ACCCTTCAGT
    TGCACGAAGC TCACCCTGTC CCAGTGGTTC TGGTGCCTCT TCATTGGTAT TGGAGAGCTG
    CTGTGGGGCC AGGTCATTTC CACGATACCT ACCCAATCCC TGAAGTTCCT GAAGGAGGCT
    GGGCATGGCA CCACCAAAGA GGAGATCACC AAGGATGCTG AGGGGCTGGA CGAAATCGAC
    CATGCAGAGA TGGAGCTGCG CCGAGGCCAG ATCCTCTGGT TCCGGGGCCT GAACCGTATC
    CAGACTCAGA TCGACGTAAT TAACACATTC CAGACGGGAG CCTCTTTTAA GGGAGTCCTA
    AAGCGCCAGA CCATGGGTCA ACACCTTGAT GTAAAACATG TTCCTAGCTC ATCCTATGTA
    ACAGTTGCAC CAGTCAAATC TCCCCCCACC ACTTCTGTTG CTGCTGCTGT TTCATCTCCT
    ACTCTGGGCA ATCAAAGTGG TCAAAGCGTT CCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223431
    Clone ID Related Accession (Same CDS sequence) XM_024976048.1
    Accession Version XM_024976048.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3498bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product plasma membrane calcium-transporting ATPase 4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGACGAACC CAACAGAACA CACCTTGCCG TCCAACTCGA TACTTGAGAG CCGTGAAGGG 
    GAGTTTGGCT GCACAGTGAT GGACCTGAGG AAGCTGATGG AGCTGCGCTC AAGTGATGCA
    ATAGACCAGA TCAACGTCCA CTACGGAGGA GTGATGAACC TCTGCAGTAG ACTGAAAACC
    AACCCTGTGG AAGGCCTATC TGGGAACCCT GCAGATCTGG AGAAACGTAA ACAAGTGTTT
    GGACAGAACT TGATTCCCCC CAAAAAGCCC AAGACCTTCT TAGAGTTAGT GTGGGAAGCC
    CTCCAGGATG TCACGCTCAT CATCCTGGAG ATTGCGGCCA TCATCTCCCT GGTCCTGTCC
    TTCTACCGCC CCCCGGGGGG AGAAAATGAA CAGTGTGGTC TAGCCGTCAC TAGCCCAGAG
    GACGAAGGGG AGGCGGAAGC TGGCTGGATT GAGGGTGCAG CCATCTTATT CTCGGTGATC
    ATCGTCGTGC TCGTGACCGC CTTCAATGAC TGGAGCAAGG AGAAGCAATT CCGGGGGCTG
    CAGAACCGCA TCGAAAAGGA GCAGAAATTC TCCGTTATCC GGAATGGCCA CATCATTCAG
    CTCCCTGTGG CTGAGATTGT GGTGGGCGAC ATTGCCCAAA TCAAATACGG TGACCTGCTG
    CCTGCAGATG GGATCCTGAT CCAGGGCAAC GATCTCAAGA TTGATGAGAG TTCTCTGACT
    GGGGAATCAG ACCATGTCAA GAAGTCCTTG GAGAGGGACC CCATGCTGCT CTCAGGGACC
    CATGTAATGG AAGGTTCTGG ACGAATGGTA GTGACGGCTG TGGGTATCAA CTCTCAGACT
    GGAATCATTT TTACCCTCTT GGGAGCCAGT GAGGGGGAAG AGGAAGAGAA GAAGAAGAAA
    GGTAAAAAAC AAGGAGTCCC TGAAAATCGC AACAAAGCAA AGACCCAAGA TGGAGTGGCC
    TTGGAAATCC AGCCACTCAA CAGCCAGGAG GGGATCGACA GTGAGGAAAA GGAGAAGAAG
    GCCGCCAAGC TGCCCAAAAA GGAGAAGTCC GTGCTGCAGG GCAAGCTGAC CCGCCTGGCT
    GTCCAGATTG GGAAAGCTGG TCTGATCATG TCTGCAATCA CAGTTCTCAT CCTGATTCTG
    TACTTCGTGA TCGACAACTT TGTGATCCAG CGCAGACCGT GGCTGGCCGA GTGTACCCCC
    ATCTACGTCC AGTACTTTGT CAAGTTCTTC ATCATCGGCG TCACGGTGCT GGTGGTGGCT
    GTGCCCGAGG GGCTGCCCCT GGCCGTCACC ATCTCACTGG CTTACTCTGT GAAGAAAATG
    ATGAAGGACA ACAACCTGGT GCGGCACTTG GACGCGTGTG AGACCATGGG CAACGCCACA
    GCCATCTGCT CGGACAAGAC GGGCACGCTG ACCATGAACC GCATGTCTGT GGTGCAGGCT
    TACATTGGAG ACACTCGTTA CCATCAGATC CCAAGCCCTG ATGACCTTGT GCCCAAGGTC
    CTGGACCTTA TTGTCAATGG CATTTCCATC AACAGTGCCT ACACCTCTAA GATTCTGCCT
    CCGGAGAAGG AGGGCGGCCT GCCCCGGCAG GTGGGCAACA AGACCGAGTG CGCCCTGCTG
    GGCTTCGTCT CGGACCTGAA GCAGGATTAC CACGCCGTGC GCAGCGAGGT GCCCGAGGAG
    AAGCTCTACA AAGTGTACAC CTTCAACTCG GTGCGCAAGT CCATGAGCAC GGTCATCGAG
    AAGCCGGGCG GCGGCTACCG CATGTACAGC AAGGGCGCCT CCGAGATCAT CCTGCGCAAG
    TGTAATCGAA TCCTGGACAA AAAGGGGGAG GCCGTGCCAT TCAAGAATAA GGACCGAGAT
    GAGATGGTCC GCACCGTCAT CGAGCCCATG GCCTGTGAGG GGCTGCGGAC ACTCTGCATC
    GCTTACCGGG ATTTCAACGA TGGAGAGCCC CCGTGGGACA ACGAGAGCGA GATCCTCACT
    GAGTTGACCT GTATCGCAGT GGTGGGCATC GAGGACCCCG TGCGCCCGGA GGTGCCAGAA
    GCTATTGCCA AATGCAAGCG AGCTGGCATC ACTGTCAGGA TGGTGACAGG GGACAACATC
    AACACAGCCC GTGCCATCGC CACCAAGTGT GGCATCGTGA CTCCCGGGGA TGACTTTCTG
    TGCTTAGAAG GCAAAGAATT CAATCGCCTC ATCCGAAACG AGAAGGGCGA GGTAGAGCAG
    GAAAAGCTGG ACAAGATCTG GCCTAAGCTC CGAGTGCTGG CGCGGTCTTC CCCCACTGAC
    AAGCACACGC TGGTGAAAGG CATCATCGAC AGCACTGTCG GCGACCAGCG GCAGGTGGTG
    GCCGTCACCG GCGACGGCAC CAACGACGGC CCGGCTCTGA AGAAAGCAGA CGTTGGTTTT
    GCCATGGGTA TTGCAGGCAC AGATGTGGCA AAGGAGGCCT CCGACATCAT CCTAACAGAC
    GACAACTTCA CCAGCATCGT GAAGGCGGTG ATGTGGGGAC GGAACGTCTA CGACAGCATC
    TCCAAGTTCC TGCAGTTCCA GCTCACCGTC AACGTGGTGG CCGTGATCGT GGCCTTCACT
    GGAGCCTGCA TCACCCAGGA TTCCCCACTG AAGGCCGTGC AGATGTTGTG GGTTAATCTA
    ATCATGGACA CCTTTGCCTC GCTGGCCCTG GCCACAGAGC CTCCTACAGA CTCTCTACTG
    AAGCGCCGGC CCTACGGCCG CAATAAGCCT CTAATCTCGC GCACCATGAT GAAGAACATC
    CTGGGTCACG CCGTCTATCA GCTCACCGTC ATCTTTTTCC TTGTGTTTGC GGGTGAGAAA
    TTCTTTGACA TTGACAGCGG GAGGAGAGCG CCTCTGCATT CTCCGCCCAG CCAGCACTAC
    ACCATTATTT TCAACACCTT CGTGCTCATG CAGCTCTTCA ACGAAATCAA CTCCCGCAAG
    ATCCACGGGG AGAGGAATGT CTTCTCGGGC ATCTTCCGCA ATCTCATCTT CTGCTCAGTG
    GTGTTGGGCA CATTCATCAG CCAGATCATC ATCGTGGAAT TCGGGGGCAA ACCCTTCAGT
    TGCACGAAGC TCACCCTGTC CCAGTGGTTC TGGTGCCTCT TCATTGGTAT TGGAGAGCTG
    CTGTGGGGCC AGGTCATTTC CACGATACCT ACCCAATCCC TGAAGTTCCT GAAGGAGGCT
    GGGCATGGCA CCACCAAAGA GGAGATCACC AAGGATGCTG AGGGGCTGGA CGAAATCGAC
    CATGCAGAGA TGGAGCTGCG CCGAGGCCAG ATCCTCTGGT TCCGGGGCCT GAACCGTATC
    CAGACTCAGA TCGACGTAAT TAACACATTC CAGACGGGAG CCTCTTTTAA GGGAGTCCTA
    AAGCGCCAGA CCATGGGTCA ACACCTTGAT GTAAAACATG TTCCTAGCTC ATCCTATGTA
    ACAGTTGCAC CAGTCAAATC TCCCCCCACC ACTTCTGTTG CTGCTGCTGT TTCATCTCCT
    ACTCTGGGCA GTGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831816.1
    CDS939..4436
    Translation

    Target ORF information:

    RefSeq Version XM_024976048.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ATPase plasma membrane Ca2+ transporting 4 (ATP2B4), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976048.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGACGAACC CAACAGAACA CACCTTGCCG TCCAACTCGA TACTTGAGAG CCGTGAAGGG 
    GAGTTTGGCT GCACAGTGAT GGACCTGAGG AAGCTGATGG AGCTGCGCTC AAGTGATGCA
    ATAGACCAGA TCAACGTCCA CTACGGAGGA GTGATGAACC TCTGCAGTAG ACTGAAAACC
    AACCCTGTGG AAGGCCTATC TGGGAACCCT GCAGATCTGG AGAAACGTAA ACAAGTGTTT
    GGACAGAACT TGATTCCCCC CAAAAAGCCC AAGACCTTCT TAGAGTTAGT GTGGGAAGCC
    CTCCAGGATG TCACGCTCAT CATCCTGGAG ATTGCGGCCA TCATCTCCCT GGTCCTGTCC
    TTCTACCGCC CCCCGGGGGG AGAAAATGAA CAGTGTGGTC TAGCCGTCAC TAGCCCAGAG
    GACGAAGGGG AGGCGGAAGC TGGCTGGATT GAGGGTGCAG CCATCTTATT CTCGGTGATC
    ATCGTCGTGC TCGTGACCGC CTTCAATGAC TGGAGCAAGG AGAAGCAATT CCGGGGGCTG
    CAGAACCGCA TCGAAAAGGA GCAGAAATTC TCCGTTATCC GGAATGGCCA CATCATTCAG
    CTCCCTGTGG CTGAGATTGT GGTGGGCGAC ATTGCCCAAA TCAAATACGG TGACCTGCTG
    CCTGCAGATG GGATCCTGAT CCAGGGCAAC GATCTCAAGA TTGATGAGAG TTCTCTGACT
    GGGGAATCAG ACCATGTCAA GAAGTCCTTG GAGAGGGACC CCATGCTGCT CTCAGGGACC
    CATGTAATGG AAGGTTCTGG ACGAATGGTA GTGACGGCTG TGGGTATCAA CTCTCAGACT
    GGAATCATTT TTACCCTCTT GGGAGCCAGT GAGGGGGAAG AGGAAGAGAA GAAGAAGAAA
    GGTAAAAAAC AAGGAGTCCC TGAAAATCGC AACAAAGCAA AGACCCAAGA TGGAGTGGCC
    TTGGAAATCC AGCCACTCAA CAGCCAGGAG GGGATCGACA GTGAGGAAAA GGAGAAGAAG
    GCCGCCAAGC TGCCCAAAAA GGAGAAGTCC GTGCTGCAGG GCAAGCTGAC CCGCCTGGCT
    GTCCAGATTG GGAAAGCTGG TCTGATCATG TCTGCAATCA CAGTTCTCAT CCTGATTCTG
    TACTTCGTGA TCGACAACTT TGTGATCCAG CGCAGACCGT GGCTGGCCGA GTGTACCCCC
    ATCTACGTCC AGTACTTTGT CAAGTTCTTC ATCATCGGCG TCACGGTGCT GGTGGTGGCT
    GTGCCCGAGG GGCTGCCCCT GGCCGTCACC ATCTCACTGG CTTACTCTGT GAAGAAAATG
    ATGAAGGACA ACAACCTGGT GCGGCACTTG GACGCGTGTG AGACCATGGG CAACGCCACA
    GCCATCTGCT CGGACAAGAC GGGCACGCTG ACCATGAACC GCATGTCTGT GGTGCAGGCT
    TACATTGGAG ACACTCGTTA CCATCAGATC CCAAGCCCTG ATGACCTTGT GCCCAAGGTC
    CTGGACCTTA TTGTCAATGG CATTTCCATC AACAGTGCCT ACACCTCTAA GATTCTGCCT
    CCGGAGAAGG AGGGCGGCCT GCCCCGGCAG GTGGGCAACA AGACCGAGTG CGCCCTGCTG
    GGCTTCGTCT CGGACCTGAA GCAGGATTAC CACGCCGTGC GCAGCGAGGT GCCCGAGGAG
    AAGCTCTACA AAGTGTACAC CTTCAACTCG GTGCGCAAGT CCATGAGCAC GGTCATCGAG
    AAGCCGGGCG GCGGCTACCG CATGTACAGC AAGGGCGCCT CCGAGATCAT CCTGCGCAAG
    TGTAATCGAA TCCTGGACAA AAAGGGGGAG GCCGTGCCAT TCAAGAATAA GGACCGAGAT
    GAGATGGTCC GCACCGTCAT CGAGCCCATG GCCTGTGAGG GGCTGCGGAC ACTCTGCATC
    GCTTACCGGG ATTTCAACGA TGGAGAGCCC CCGTGGGACA ACGAGAGCGA GATCCTCACT
    GAGTTGACCT GTATCGCAGT GGTGGGCATC GAGGACCCCG TGCGCCCGGA GGTGCCAGAA
    GCTATTGCCA AATGCAAGCG AGCTGGCATC ACTGTCAGGA TGGTGACAGG GGACAACATC
    AACACAGCCC GTGCCATCGC CACCAAGTGT GGCATCGTGA CTCCCGGGGA TGACTTTCTG
    TGCTTAGAAG GCAAAGAATT CAATCGCCTC ATCCGAAACG AGAAGGGCGA GGTAGAGCAG
    GAAAAGCTGG ACAAGATCTG GCCTAAGCTC CGAGTGCTGG CGCGGTCTTC CCCCACTGAC
    AAGCACACGC TGGTGAAAGG CATCATCGAC AGCACTGTCG GCGACCAGCG GCAGGTGGTG
    GCCGTCACCG GCGACGGCAC CAACGACGGC CCGGCTCTGA AGAAAGCAGA CGTTGGTTTT
    GCCATGGGTA TTGCAGGCAC AGATGTGGCA AAGGAGGCCT CCGACATCAT CCTAACAGAC
    GACAACTTCA CCAGCATCGT GAAGGCGGTG ATGTGGGGAC GGAACGTCTA CGACAGCATC
    TCCAAGTTCC TGCAGTTCCA GCTCACCGTC AACGTGGTGG CCGTGATCGT GGCCTTCACT
    GGAGCCTGCA TCACCCAGGA TTCCCCACTG AAGGCCGTGC AGATGTTGTG GGTTAATCTA
    ATCATGGACA CCTTTGCCTC GCTGGCCCTG GCCACAGAGC CTCCTACAGA CTCTCTACTG
    AAGCGCCGGC CCTACGGCCG CAATAAGCCT CTAATCTCGC GCACCATGAT GAAGAACATC
    CTGGGTCACG CCGTCTATCA GCTCACCGTC ATCTTTTTCC TTGTGTTTGC GGGTGAGAAA
    TTCTTTGACA TTGACAGCGG GAGGAGAGCG CCTCTGCATT CTCCGCCCAG CCAGCACTAC
    ACCATTATTT TCAACACCTT CGTGCTCATG CAGCTCTTCA ACGAAATCAA CTCCCGCAAG
    ATCCACGGGG AGAGGAATGT CTTCTCGGGC ATCTTCCGCA ATCTCATCTT CTGCTCAGTG
    GTGTTGGGCA CATTCATCAG CCAGATCATC ATCGTGGAAT TCGGGGGCAA ACCCTTCAGT
    TGCACGAAGC TCACCCTGTC CCAGTGGTTC TGGTGCCTCT TCATTGGTAT TGGAGAGCTG
    CTGTGGGGCC AGGTCATTTC CACGATACCT ACCCAATCCC TGAAGTTCCT GAAGGAGGCT
    GGGCATGGCA CCACCAAAGA GGAGATCACC AAGGATGCTG AGGGGCTGGA CGAAATCGAC
    CATGCAGAGA TGGAGCTGCG CCGAGGCCAG ATCCTCTGGT TCCGGGGCCT GAACCGTATC
    CAGACTCAGA TCGACGTAAT TAACACATTC CAGACGGGAG CCTCTTTTAA GGGAGTCCTA
    AAGCGCCAGA CCATGGGTCA ACACCTTGAT GTAAAACATG TTCCTAGCTC ATCCTATGTA
    ACAGTTGCAC CAGTCAAATC TCCCCCCACC ACTTCTGTTG CTGCTGCTGT TTCATCTCCT
    ACTCTGGGCA GTGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223432
    Clone ID Related Accession (Same CDS sequence) XM_024976049.1
    Accession Version XM_024976049.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3480bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product plasma membrane calcium-transporting ATPase 4 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGACGAACC CAACAGAACA CACCTTGCCG TCCAACTCGA TACTTGAGAG CCGTGAAGGG 
    GAGTTTGGCT GCACAGTGAT GGACCTGAGG AAGCTGATGG AGCTGCGCTC AAGTGATGCA
    ATAGACCAGA TCAACGTCCA CTACGGAGGA GTGATGAACC TCTGCAGTAG ACTGAAAACC
    AACCCTGTGG AAGGCCTATC TGGGAACCCT GCAGATCTGG AGAAACGTAA ACAAGTGTTT
    GGACAGAACT TGATTCCCCC CAAAAAGCCC AAGACCTTCT TAGAGTTAGT GTGGGAAGCC
    CTCCAGGATG TCACGCTCAT CATCCTGGAG ATTGCGGCCA TCATCTCCCT GGTCCTGTCC
    TTCTACCGCC CCCCGGGGGG AGAAAATGAA CAGTGTGGTC TAGCCGTCAC TAGCCCAGAG
    GACGAAGGGG AGGCGGAAGC TGGCTGGATT GAGGGTGCAG CCATCTTATT CTCGGTGATC
    ATCGTCGTGC TCGTGACCGC CTTCAATGAC TGGAGCAAGG AGAAGCAATT CCGGGGGCTG
    CAGAACCGCA TCGAAAAGGA GCAGAAATTC TCCGTTATCC GGAATGGCCA CATCATTCAG
    CTCCCTGTGG CTGAGATTGT GGTGGGCGAC ATTGCCCAAA TCAAATACGG TGACCTGCTG
    CCTGCAGATG GGATCCTGAT CCAGGGCAAC GATCTCAAGA TTGATGAGAG TTCTCTGACT
    GGGGAATCAG ACCATGTCAA GAAGTCCTTG GAGAGGGACC CCATGCTGCT CTCAGGGACC
    CATGTAATGG AAGGTTCTGG ACGAATGGTA GTGACGGCTG TGGGTATCAA CTCTCAGACT
    GGAATCATTT TTACCCTCTT GGGAGCCAGT GAGGGGGAAG AGGAAGAGAA GAAGAAGAAA
    GCAAAGACCC AAGATGGAGT GGCCTTGGAA ATCCAGCCAC TCAACAGCCA GGAGGGGATC
    GACAGTGAGG AAAAGGAGAA GAAGGCCGCC AAGCTGCCCA AAAAGGAGAA GTCCGTGCTG
    CAGGGCAAGC TGACCCGCCT GGCTGTCCAG ATTGGGAAAG CTGGTCTGAT CATGTCTGCA
    ATCACAGTTC TCATCCTGAT TCTGTACTTC GTGATCGACA ACTTTGTGAT CCAGCGCAGA
    CCGTGGCTGG CCGAGTGTAC CCCCATCTAC GTCCAGTACT TTGTCAAGTT CTTCATCATC
    GGCGTCACGG TGCTGGTGGT GGCTGTGCCC GAGGGGCTGC CCCTGGCCGT CACCATCTCA
    CTGGCTTACT CTGTGAAGAA AATGATGAAG GACAACAACC TGGTGCGGCA CTTGGACGCG
    TGTGAGACCA TGGGCAACGC CACAGCCATC TGCTCGGACA AGACGGGCAC GCTGACCATG
    AACCGCATGT CTGTGGTGCA GGCTTACATT GGAGACACTC GTTACCATCA GATCCCAAGC
    CCTGATGACC TTGTGCCCAA GGTCCTGGAC CTTATTGTCA ATGGCATTTC CATCAACAGT
    GCCTACACCT CTAAGATTCT GCCTCCGGAG AAGGAGGGCG GCCTGCCCCG GCAGGTGGGC
    AACAAGACCG AGTGCGCCCT GCTGGGCTTC GTCTCGGACC TGAAGCAGGA TTACCACGCC
    GTGCGCAGCG AGGTGCCCGA GGAGAAGCTC TACAAAGTGT ACACCTTCAA CTCGGTGCGC
    AAGTCCATGA GCACGGTCAT CGAGAAGCCG GGCGGCGGCT ACCGCATGTA CAGCAAGGGC
    GCCTCCGAGA TCATCCTGCG CAAGTGTAAT CGAATCCTGG ACAAAAAGGG GGAGGCCGTG
    CCATTCAAGA ATAAGGACCG AGATGAGATG GTCCGCACCG TCATCGAGCC CATGGCCTGT
    GAGGGGCTGC GGACACTCTG CATCGCTTAC CGGGATTTCA ACGATGGAGA GCCCCCGTGG
    GACAACGAGA GCGAGATCCT CACTGAGTTG ACCTGTATCG CAGTGGTGGG CATCGAGGAC
    CCCGTGCGCC CGGAGGTGCC AGAAGCTATT GCCAAATGCA AGCGAGCTGG CATCACTGTC
    AGGATGGTGA CAGGGGACAA CATCAACACA GCCCGTGCCA TCGCCACCAA GTGTGGCATC
    GTGACTCCCG GGGATGACTT TCTGTGCTTA GAAGGCAAAG AATTCAATCG CCTCATCCGA
    AACGAGAAGG GCGAGGTAGA GCAGGAAAAG CTGGACAAGA TCTGGCCTAA GCTCCGAGTG
    CTGGCGCGGT CTTCCCCCAC TGACAAGCAC ACGCTGGTGA AAGGCATCAT CGACAGCACT
    GTCGGCGACC AGCGGCAGGT GGTGGCCGTC ACCGGCGACG GCACCAACGA CGGCCCGGCT
    CTGAAGAAAG CAGACGTTGG TTTTGCCATG GGTATTGCAG GCACAGATGT GGCAAAGGAG
    GCCTCCGACA TCATCCTAAC AGACGACAAC TTCACCAGCA TCGTGAAGGC GGTGATGTGG
    GGACGGAACG TCTACGACAG CATCTCCAAG TTCCTGCAGT TCCAGCTCAC CGTCAACGTG
    GTGGCCGTGA TCGTGGCCTT CACTGGAGCC TGCATCACCC AGGATTCCCC ACTGAAGGCC
    GTGCAGATGT TGTGGGTTAA TCTAATCATG GACACCTTTG CCTCGCTGGC CCTGGCCACA
    GAGCCTCCTA CAGACTCTCT ACTGAAGCGC CGGCCCTACG GCCGCAATAA GCCTCTAATC
    TCGCGCACCA TGATGAAGAA CATCCTGGGT CACGCCGTCT ATCAGCTCAC CGTCATCTTT
    TTCCTTGTGT TTGCGGGTGA GAAATTCTTT GACATTGACA GCGGGAGGAG AGCGCCTCTG
    CATTCTCCGC CCAGCCAGCA CTACACCATT ATTTTCAACA CCTTCGTGCT CATGCAGCTC
    TTCAACGAAA TCAACTCCCG CAAGATCCAC GGGGAGAGGA ATGTCTTCTC GGGCATCTTC
    CGCAATCTCA TCTTCTGCTC AGTGGTGTTG GGCACATTCA TCAGCCAGAT CATCATCGTG
    GAATTCGGGG GCAAACCCTT CAGTTGCACG AAGCTCACCC TGTCCCAGTG GTTCTGGTGC
    CTCTTCATTG GTATTGGAGA GCTGCTGTGG GGCCAGGTCA TTTCCACGAT ACCTACCCAA
    TCCCTGAAGT TCCTGAAGGA GGCTGGGCAT GGCACCACCA AAGAGGAGAT CACCAAGGAT
    GCTGAGGGGC TGGACGAAAT CGACCATGCA GAGATGGAGC TGCGCCGAGG CCAGATCCTC
    TGGTTCCGGG GCCTGAACCG TATCCAGACT CAGATCGACG TAATTAACAC ATTCCAGACG
    GGAGCCTCTT TTAAGGGAGT CCTAAAGCGC CAGACCATGG GTCAACACCT TGATGTAAAA
    CATGTTCCTA GCTCATCCTA TGTAACAGTT GCACCAGTCA AATCTCCCCC CACCACTTCT
    GTTGCTGCTG CTGTTTCATC TCCTACTCTG GGCAATCAAA GTGGTCAAAG CGTTCCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831817.1
    CDS950..4429
    Translation

    Target ORF information:

    RefSeq Version XM_024976049.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ATPase plasma membrane Ca2+ transporting 4 (ATP2B4), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976049.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGACGAACC CAACAGAACA CACCTTGCCG TCCAACTCGA TACTTGAGAG CCGTGAAGGG 
    GAGTTTGGCT GCACAGTGAT GGACCTGAGG AAGCTGATGG AGCTGCGCTC AAGTGATGCA
    ATAGACCAGA TCAACGTCCA CTACGGAGGA GTGATGAACC TCTGCAGTAG ACTGAAAACC
    AACCCTGTGG AAGGCCTATC TGGGAACCCT GCAGATCTGG AGAAACGTAA ACAAGTGTTT
    GGACAGAACT TGATTCCCCC CAAAAAGCCC AAGACCTTCT TAGAGTTAGT GTGGGAAGCC
    CTCCAGGATG TCACGCTCAT CATCCTGGAG ATTGCGGCCA TCATCTCCCT GGTCCTGTCC
    TTCTACCGCC CCCCGGGGGG AGAAAATGAA CAGTGTGGTC TAGCCGTCAC TAGCCCAGAG
    GACGAAGGGG AGGCGGAAGC TGGCTGGATT GAGGGTGCAG CCATCTTATT CTCGGTGATC
    ATCGTCGTGC TCGTGACCGC CTTCAATGAC TGGAGCAAGG AGAAGCAATT CCGGGGGCTG
    CAGAACCGCA TCGAAAAGGA GCAGAAATTC TCCGTTATCC GGAATGGCCA CATCATTCAG
    CTCCCTGTGG CTGAGATTGT GGTGGGCGAC ATTGCCCAAA TCAAATACGG TGACCTGCTG
    CCTGCAGATG GGATCCTGAT CCAGGGCAAC GATCTCAAGA TTGATGAGAG TTCTCTGACT
    GGGGAATCAG ACCATGTCAA GAAGTCCTTG GAGAGGGACC CCATGCTGCT CTCAGGGACC
    CATGTAATGG AAGGTTCTGG ACGAATGGTA GTGACGGCTG TGGGTATCAA CTCTCAGACT
    GGAATCATTT TTACCCTCTT GGGAGCCAGT GAGGGGGAAG AGGAAGAGAA GAAGAAGAAA
    GCAAAGACCC AAGATGGAGT GGCCTTGGAA ATCCAGCCAC TCAACAGCCA GGAGGGGATC
    GACAGTGAGG AAAAGGAGAA GAAGGCCGCC AAGCTGCCCA AAAAGGAGAA GTCCGTGCTG
    CAGGGCAAGC TGACCCGCCT GGCTGTCCAG ATTGGGAAAG CTGGTCTGAT CATGTCTGCA
    ATCACAGTTC TCATCCTGAT TCTGTACTTC GTGATCGACA ACTTTGTGAT CCAGCGCAGA
    CCGTGGCTGG CCGAGTGTAC CCCCATCTAC GTCCAGTACT TTGTCAAGTT CTTCATCATC
    GGCGTCACGG TGCTGGTGGT GGCTGTGCCC GAGGGGCTGC CCCTGGCCGT CACCATCTCA
    CTGGCTTACT CTGTGAAGAA AATGATGAAG GACAACAACC TGGTGCGGCA CTTGGACGCG
    TGTGAGACCA TGGGCAACGC CACAGCCATC TGCTCGGACA AGACGGGCAC GCTGACCATG
    AACCGCATGT CTGTGGTGCA GGCTTACATT GGAGACACTC GTTACCATCA GATCCCAAGC
    CCTGATGACC TTGTGCCCAA GGTCCTGGAC CTTATTGTCA ATGGCATTTC CATCAACAGT
    GCCTACACCT CTAAGATTCT GCCTCCGGAG AAGGAGGGCG GCCTGCCCCG GCAGGTGGGC
    AACAAGACCG AGTGCGCCCT GCTGGGCTTC GTCTCGGACC TGAAGCAGGA TTACCACGCC
    GTGCGCAGCG AGGTGCCCGA GGAGAAGCTC TACAAAGTGT ACACCTTCAA CTCGGTGCGC
    AAGTCCATGA GCACGGTCAT CGAGAAGCCG GGCGGCGGCT ACCGCATGTA CAGCAAGGGC
    GCCTCCGAGA TCATCCTGCG CAAGTGTAAT CGAATCCTGG ACAAAAAGGG GGAGGCCGTG
    CCATTCAAGA ATAAGGACCG AGATGAGATG GTCCGCACCG TCATCGAGCC CATGGCCTGT
    GAGGGGCTGC GGACACTCTG CATCGCTTAC CGGGATTTCA ACGATGGAGA GCCCCCGTGG
    GACAACGAGA GCGAGATCCT CACTGAGTTG ACCTGTATCG CAGTGGTGGG CATCGAGGAC
    CCCGTGCGCC CGGAGGTGCC AGAAGCTATT GCCAAATGCA AGCGAGCTGG CATCACTGTC
    AGGATGGTGA CAGGGGACAA CATCAACACA GCCCGTGCCA TCGCCACCAA GTGTGGCATC
    GTGACTCCCG GGGATGACTT TCTGTGCTTA GAAGGCAAAG AATTCAATCG CCTCATCCGA
    AACGAGAAGG GCGAGGTAGA GCAGGAAAAG CTGGACAAGA TCTGGCCTAA GCTCCGAGTG
    CTGGCGCGGT CTTCCCCCAC TGACAAGCAC ACGCTGGTGA AAGGCATCAT CGACAGCACT
    GTCGGCGACC AGCGGCAGGT GGTGGCCGTC ACCGGCGACG GCACCAACGA CGGCCCGGCT
    CTGAAGAAAG CAGACGTTGG TTTTGCCATG GGTATTGCAG GCACAGATGT GGCAAAGGAG
    GCCTCCGACA TCATCCTAAC AGACGACAAC TTCACCAGCA TCGTGAAGGC GGTGATGTGG
    GGACGGAACG TCTACGACAG CATCTCCAAG TTCCTGCAGT TCCAGCTCAC CGTCAACGTG
    GTGGCCGTGA TCGTGGCCTT CACTGGAGCC TGCATCACCC AGGATTCCCC ACTGAAGGCC
    GTGCAGATGT TGTGGGTTAA TCTAATCATG GACACCTTTG CCTCGCTGGC CCTGGCCACA
    GAGCCTCCTA CAGACTCTCT ACTGAAGCGC CGGCCCTACG GCCGCAATAA GCCTCTAATC
    TCGCGCACCA TGATGAAGAA CATCCTGGGT CACGCCGTCT ATCAGCTCAC CGTCATCTTT
    TTCCTTGTGT TTGCGGGTGA GAAATTCTTT GACATTGACA GCGGGAGGAG AGCGCCTCTG
    CATTCTCCGC CCAGCCAGCA CTACACCATT ATTTTCAACA CCTTCGTGCT CATGCAGCTC
    TTCAACGAAA TCAACTCCCG CAAGATCCAC GGGGAGAGGA ATGTCTTCTC GGGCATCTTC
    CGCAATCTCA TCTTCTGCTC AGTGGTGTTG GGCACATTCA TCAGCCAGAT CATCATCGTG
    GAATTCGGGG GCAAACCCTT CAGTTGCACG AAGCTCACCC TGTCCCAGTG GTTCTGGTGC
    CTCTTCATTG GTATTGGAGA GCTGCTGTGG GGCCAGGTCA TTTCCACGAT ACCTACCCAA
    TCCCTGAAGT TCCTGAAGGA GGCTGGGCAT GGCACCACCA AAGAGGAGAT CACCAAGGAT
    GCTGAGGGGC TGGACGAAAT CGACCATGCA GAGATGGAGC TGCGCCGAGG CCAGATCCTC
    TGGTTCCGGG GCCTGAACCG TATCCAGACT CAGATCGACG TAATTAACAC ATTCCAGACG
    GGAGCCTCTT TTAAGGGAGT CCTAAAGCGC CAGACCATGG GTCAACACCT TGATGTAAAA
    CATGTTCCTA GCTCATCCTA TGTAACAGTT GCACCAGTCA AATCTCCCCC CACCACTTCT
    GTTGCTGCTG CTGTTTCATC TCCTACTCTG GGCAATCAAA GTGGTCAAAG CGTTCCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223433
    Clone ID Related Accession (Same CDS sequence) XM_024976050.1
    Accession Version XM_024976050.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3426bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product plasma membrane calcium-transporting ATPase 4 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGACGAACC CAACAGAACA CACCTTGCCG TCCAACTCGA TACTTGAGAG CCGTGAAGGG 
    GAGTTTGGCT GCACAGTGAT GGACCTGAGG AAGCTGATGG AGCTGCGCTC AAGTGATGCA
    ATAGACCAGA TCAACGTCCA CTACGGAGGA GTGATGAACC TCTGCAGTAG ACTGAAAACC
    AACCCTGTGG AAGAGTGTGG TCTAGCCGTC ACTAGCCCAG AGGACGAAGG GGAGGCGGAA
    GCTGGCTGGA TTGAGGGTGC AGCCATCTTA TTCTCGGTGA TCATCGTCGT GCTCGTGACC
    GCCTTCAATG ACTGGAGCAA GGAGAAGCAA TTCCGGGGGC TGCAGAACCG CATCGAAAAG
    GAGCAGAAAT TCTCCGTTAT CCGGAATGGC CACATCATTC AGCTCCCTGT GGCTGAGATT
    GTGGTGGGCG ACATTGCCCA AATCAAATAC GGTGACCTGC TGCCTGCAGA TGGGATCCTG
    ATCCAGGGCA ACGATCTCAA GATTGATGAG AGTTCTCTGA CTGGGGAATC AGACCATGTC
    AAGAAGTCCT TGGAGAGGGA CCCCATGCTG CTCTCAGGGA CCCATGTAAT GGAAGGTTCT
    GGACGAATGG TAGTGACGGC TGTGGGTATC AACTCTCAGA CTGGAATCAT TTTTACCCTC
    TTGGGAGCCA GTGAGGGGGA AGAGGAAGAG AAGAAGAAGA AAGGTAAAAA ACAAGGAGTC
    CCTGAAAATC GCAACAAAGC AAAGACCCAA GATGGAGTGG CCTTGGAAAT CCAGCCACTC
    AACAGCCAGG AGGGGATCGA CAGTGAGGAA AAGGAGAAGA AGGCCGCCAA GCTGCCCAAA
    AAGGAGAAGT CCGTGCTGCA GGGCAAGCTG ACCCGCCTGG CTGTCCAGAT TGGGAAAGCT
    GGTCTGATCA TGTCTGCAAT CACAGTTCTC ATCCTGATTC TGTACTTCGT GATCGACAAC
    TTTGTGATCC AGCGCAGACC GTGGCTGGCC GAGTGTACCC CCATCTACGT CCAGTACTTT
    GTCAAGTTCT TCATCATCGG CGTCACGGTG CTGGTGGTGG CTGTGCCCGA GGGGCTGCCC
    CTGGCCGTCA CCATCTCACT GGCTTACTCT GTGAAGAAAA TGATGAAGGA CAACAACCTG
    GTGCGGCACT TGGACGCGTG TGAGACCATG GGCAACGCCA CAGCCATCTG CTCGGACAAG
    ACGGGCACGC TGACCATGAA CCGCATGTCT GTGGTGCAGG CTTACATTGG AGACACTCGT
    TACCATCAGA TCCCAAGCCC TGATGACCTT GTGCCCAAGG TCCTGGACCT TATTGTCAAT
    GGCATTTCCA TCAACAGTGC CTACACCTCT AAGATTCTGC CTCCGGAGAA GGAGGGCGGC
    CTGCCCCGGC AGGTGGGCAA CAAGACCGAG TGCGCCCTGC TGGGCTTCGT CTCGGACCTG
    AAGCAGGATT ACCACGCCGT GCGCAGCGAG GTGCCCGAGG AGAAGCTCTA CAAAGTGTAC
    ACCTTCAACT CGGTGCGCAA GTCCATGAGC ACGGTCATCG AGAAGCCGGG CGGCGGCTAC
    CGCATGTACA GCAAGGGCGC CTCCGAGATC ATCCTGCGCA AGTGTAATCG AATCCTGGAC
    AAAAAGGGGG AGGCCGTGCC ATTCAAGAAT AAGGACCGAG ATGAGATGGT CCGCACCGTC
    ATCGAGCCCA TGGCCTGTGA GGGGCTGCGG ACACTCTGCA TCGCTTACCG GGATTTCAAC
    GATGGAGAGC CCCCGTGGGA CAACGAGAGC GAGATCCTCA CTGAGTTGAC CTGTATCGCA
    GTGGTGGGCA TCGAGGACCC CGTGCGCCCG GAGGTGCCAG AAGCTATTGC CAAATGCAAG
    CGAGCTGGCA TCACTGTCAG GATGGTGACA GGGGACAACA TCAACACAGC CCGTGCCATC
    GCCACCAAGT GTGGCATCGT GACTCCCGGG GATGACTTTC TGTGCTTAGA AGGCAAAGAA
    TTCAATCGCC TCATCCGAAA CGAGAAGGGC GAGGTAGAGC AGGAAAAGCT GGACAAGATC
    TGGCCTAAGC TCCGAGTGCT GGCGCGGTCT TCCCCCACTG ACAAGCACAC GCTGGTGAAA
    GGCATCATCG ACAGCACTGT CGGCGACCAG CGGCAGGTGG TGGCCGTCAC CGGCGACGGC
    ACCAACGACG GCCCGGCTCT GAAGAAAGCA GACGTTGGTT TTGCCATGGG TATTGCAGGC
    ACAGATGTGG CAAAGGAGGC CTCCGACATC ATCCTAACAG ACGACAACTT CACCAGCATC
    GTGAAGGCGG TGATGTGGGG ACGGAACGTC TACGACAGCA TCTCCAAGTT CCTGCAGTTC
    CAGCTCACCG TCAACGTGGT GGCCGTGATC GTGGCCTTCA CTGGAGCCTG CATCACCCAG
    GATTCCCCAC TGAAGGCCGT GCAGATGTTG TGGGTTAATC TAATCATGGA CACCTTTGCC
    TCGCTGGCCC TGGCCACAGA GCCTCCTACA GACTCTCTAC TGAAGCGCCG GCCCTACGGC
    CGCAATAAGC CTCTAATCTC GCGCACCATG ATGAAGAACA TCCTGGGTCA CGCCGTCTAT
    CAGCTCACCG TCATCTTTTT CCTTGTGTTT GCGGGTGAGA AATTCTTTGA CATTGACAGC
    GGGAGGAGAG CGCCTCTGCA TTCTCCGCCC AGCCAGCACT ACACCATTAT TTTCAACACC
    TTCGTGCTCA TGCAGCTCTT CAACGAAATC AACTCCCGCA AGATCCACGG GGAGAGGAAT
    GTCTTCTCGG GCATCTTCCG CAATCTCATC TTCTGCTCAG TGGTGTTGGG CACATTCATC
    AGCCAGATCA TCATCGTGGA ATTCGGGGGC AAACCCTTCA GTTGCACGAA GCTCACCCTG
    TCCCAGTGGT TCTGGTGCCT CTTCATTGGT ATTGGAGAGC TGCTGTGGGG CCAGGTCATT
    TCCACGATAC CTACCCAATC CCTGAAGTTC CTGAAGGAGG CTGGGCATGG CACCACCAAA
    GAGGAGATCA CCAAGGATGC TGAGGGGCTG GACGAAATCG ACCATGCAGA GATGGAGCTG
    CGCCGAGGCC AGATCCTCTG GTTCCGGGGC CTGAACCGTA TCCAGACTCA GATCAAAGTG
    GTCAAAGCGT TCCATAGTTC CCTCCACGAA AGCATTCAGA AACCCAAGAA CCAAAACTCC
    ATCCACAACT TCATGACCCA CCCTGAATTC ACCATAGATG AGGAGGGGCC ACGAACACCA
    CTCCTGGATG AGCAAGAAGA GGAGATTTTT GAAAAGGTGT CTAAGCCTGG GACTAAGACG
    TCCTCACTGG ATGGTGAGGT CACTCCACAA ACCAACAAAA ACAACAATAC GGTGGATTGC
    TGCCAAGTAC AAATTGTTGC TTCCCACTCG GACAGCCCTC TACACAGCCT GGAGACGTCC
    GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831818.1
    CDS931..4356
    Translation

    Target ORF information:

    RefSeq Version XM_024976050.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ATPase plasma membrane Ca2+ transporting 4 (ATP2B4), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976050.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGACGAACC CAACAGAACA CACCTTGCCG TCCAACTCGA TACTTGAGAG CCGTGAAGGG 
    GAGTTTGGCT GCACAGTGAT GGACCTGAGG AAGCTGATGG AGCTGCGCTC AAGTGATGCA
    ATAGACCAGA TCAACGTCCA CTACGGAGGA GTGATGAACC TCTGCAGTAG ACTGAAAACC
    AACCCTGTGG AAGAGTGTGG TCTAGCCGTC ACTAGCCCAG AGGACGAAGG GGAGGCGGAA
    GCTGGCTGGA TTGAGGGTGC AGCCATCTTA TTCTCGGTGA TCATCGTCGT GCTCGTGACC
    GCCTTCAATG ACTGGAGCAA GGAGAAGCAA TTCCGGGGGC TGCAGAACCG CATCGAAAAG
    GAGCAGAAAT TCTCCGTTAT CCGGAATGGC CACATCATTC AGCTCCCTGT GGCTGAGATT
    GTGGTGGGCG ACATTGCCCA AATCAAATAC GGTGACCTGC TGCCTGCAGA TGGGATCCTG
    ATCCAGGGCA ACGATCTCAA GATTGATGAG AGTTCTCTGA CTGGGGAATC AGACCATGTC
    AAGAAGTCCT TGGAGAGGGA CCCCATGCTG CTCTCAGGGA CCCATGTAAT GGAAGGTTCT
    GGACGAATGG TAGTGACGGC TGTGGGTATC AACTCTCAGA CTGGAATCAT TTTTACCCTC
    TTGGGAGCCA GTGAGGGGGA AGAGGAAGAG AAGAAGAAGA AAGGTAAAAA ACAAGGAGTC
    CCTGAAAATC GCAACAAAGC AAAGACCCAA GATGGAGTGG CCTTGGAAAT CCAGCCACTC
    AACAGCCAGG AGGGGATCGA CAGTGAGGAA AAGGAGAAGA AGGCCGCCAA GCTGCCCAAA
    AAGGAGAAGT CCGTGCTGCA GGGCAAGCTG ACCCGCCTGG CTGTCCAGAT TGGGAAAGCT
    GGTCTGATCA TGTCTGCAAT CACAGTTCTC ATCCTGATTC TGTACTTCGT GATCGACAAC
    TTTGTGATCC AGCGCAGACC GTGGCTGGCC GAGTGTACCC CCATCTACGT CCAGTACTTT
    GTCAAGTTCT TCATCATCGG CGTCACGGTG CTGGTGGTGG CTGTGCCCGA GGGGCTGCCC
    CTGGCCGTCA CCATCTCACT GGCTTACTCT GTGAAGAAAA TGATGAAGGA CAACAACCTG
    GTGCGGCACT TGGACGCGTG TGAGACCATG GGCAACGCCA CAGCCATCTG CTCGGACAAG
    ACGGGCACGC TGACCATGAA CCGCATGTCT GTGGTGCAGG CTTACATTGG AGACACTCGT
    TACCATCAGA TCCCAAGCCC TGATGACCTT GTGCCCAAGG TCCTGGACCT TATTGTCAAT
    GGCATTTCCA TCAACAGTGC CTACACCTCT AAGATTCTGC CTCCGGAGAA GGAGGGCGGC
    CTGCCCCGGC AGGTGGGCAA CAAGACCGAG TGCGCCCTGC TGGGCTTCGT CTCGGACCTG
    AAGCAGGATT ACCACGCCGT GCGCAGCGAG GTGCCCGAGG AGAAGCTCTA CAAAGTGTAC
    ACCTTCAACT CGGTGCGCAA GTCCATGAGC ACGGTCATCG AGAAGCCGGG CGGCGGCTAC
    CGCATGTACA GCAAGGGCGC CTCCGAGATC ATCCTGCGCA AGTGTAATCG AATCCTGGAC
    AAAAAGGGGG AGGCCGTGCC ATTCAAGAAT AAGGACCGAG ATGAGATGGT CCGCACCGTC
    ATCGAGCCCA TGGCCTGTGA GGGGCTGCGG ACACTCTGCA TCGCTTACCG GGATTTCAAC
    GATGGAGAGC CCCCGTGGGA CAACGAGAGC GAGATCCTCA CTGAGTTGAC CTGTATCGCA
    GTGGTGGGCA TCGAGGACCC CGTGCGCCCG GAGGTGCCAG AAGCTATTGC CAAATGCAAG
    CGAGCTGGCA TCACTGTCAG GATGGTGACA GGGGACAACA TCAACACAGC CCGTGCCATC
    GCCACCAAGT GTGGCATCGT GACTCCCGGG GATGACTTTC TGTGCTTAGA AGGCAAAGAA
    TTCAATCGCC TCATCCGAAA CGAGAAGGGC GAGGTAGAGC AGGAAAAGCT GGACAAGATC
    TGGCCTAAGC TCCGAGTGCT GGCGCGGTCT TCCCCCACTG ACAAGCACAC GCTGGTGAAA
    GGCATCATCG ACAGCACTGT CGGCGACCAG CGGCAGGTGG TGGCCGTCAC CGGCGACGGC
    ACCAACGACG GCCCGGCTCT GAAGAAAGCA GACGTTGGTT TTGCCATGGG TATTGCAGGC
    ACAGATGTGG CAAAGGAGGC CTCCGACATC ATCCTAACAG ACGACAACTT CACCAGCATC
    GTGAAGGCGG TGATGTGGGG ACGGAACGTC TACGACAGCA TCTCCAAGTT CCTGCAGTTC
    CAGCTCACCG TCAACGTGGT GGCCGTGATC GTGGCCTTCA CTGGAGCCTG CATCACCCAG
    GATTCCCCAC TGAAGGCCGT GCAGATGTTG TGGGTTAATC TAATCATGGA CACCTTTGCC
    TCGCTGGCCC TGGCCACAGA GCCTCCTACA GACTCTCTAC TGAAGCGCCG GCCCTACGGC
    CGCAATAAGC CTCTAATCTC GCGCACCATG ATGAAGAACA TCCTGGGTCA CGCCGTCTAT
    CAGCTCACCG TCATCTTTTT CCTTGTGTTT GCGGGTGAGA AATTCTTTGA CATTGACAGC
    GGGAGGAGAG CGCCTCTGCA TTCTCCGCCC AGCCAGCACT ACACCATTAT TTTCAACACC
    TTCGTGCTCA TGCAGCTCTT CAACGAAATC AACTCCCGCA AGATCCACGG GGAGAGGAAT
    GTCTTCTCGG GCATCTTCCG CAATCTCATC TTCTGCTCAG TGGTGTTGGG CACATTCATC
    AGCCAGATCA TCATCGTGGA ATTCGGGGGC AAACCCTTCA GTTGCACGAA GCTCACCCTG
    TCCCAGTGGT TCTGGTGCCT CTTCATTGGT ATTGGAGAGC TGCTGTGGGG CCAGGTCATT
    TCCACGATAC CTACCCAATC CCTGAAGTTC CTGAAGGAGG CTGGGCATGG CACCACCAAA
    GAGGAGATCA CCAAGGATGC TGAGGGGCTG GACGAAATCG ACCATGCAGA GATGGAGCTG
    CGCCGAGGCC AGATCCTCTG GTTCCGGGGC CTGAACCGTA TCCAGACTCA GATCAAAGTG
    GTCAAAGCGT TCCATAGTTC CCTCCACGAA AGCATTCAGA AACCCAAGAA CCAAAACTCC
    ATCCACAACT TCATGACCCA CCCTGAATTC ACCATAGATG AGGAGGGGCC ACGAACACCA
    CTCCTGGATG AGCAAGAAGA GGAGATTTTT GAAAAGGTGT CTAAGCCTGG GACTAAGACG
    TCCTCACTGG ATGGTGAGGT CACTCCACAA ACCAACAAAA ACAACAATAC GGTGGATTGC
    TGCCAAGTAC AAATTGTTGC TTCCCACTCG GACAGCCCTC TACACAGCCT GGAGACGTCC
    GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223434
    Clone ID Related Accession (Same CDS sequence) XM_024976051.1
    Accession Version XM_024976051.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3732bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product plasma membrane calcium-transporting ATPase 4 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGACGAACC CAACAGAACA CACCTTGCCG TCCAACTCGA TACTTGAGAG CCGTGAAGGG 
    GAGTTTGGCT GCACAGTGAT GGACCTGAGG AAGCTGATGG AGCTGCGCTC AAGTGATGCA
    ATAGACCAGA TCAACGTCCA CTACGGAGGA GTGATGAACC TCTGCAGTAG ACTGAAAACC
    AACCCTGTGG AAGGCCTATC TGGGAACCCT GCAGATCTGG AGAAACGTAA ACAAGTGTTT
    GGACAGAACT TGATTCCCCC CAAAAAGCCC AAGACCTTCT TAGAGTTAGT GTGGGAAGCC
    CTCCAGGATG TCACGCTCAT CATCCTGGAG ATTGCGGCCA TCATCTCCCT GGTCCTGTCC
    TTCTACCGCC CCCCGGGGGG AGAAAATGAA CAGTGTGGTC TAGCCGTCAC TAGCCCAGAG
    GACGAAGGGG AGGCGGAAGC TGGCTGGATT GAGGGTGCAG CCATCTTATT CTCGGTGATC
    ATCGTCGTGC TCGTGACCGC CTTCAATGAC TGGAGCAAGG AGAAGCAATT CCGGGGGCTG
    CAGAACCGCA TCGAAAAGGA GCAGAAATTC TCCGTTATCC GGAATGGCCA CATCATTCAG
    CTCCCTGTGG CTGAGATTGT GGTGGGCGAC ATTGCCCAAA TCAAATACGG TGACCTGCTG
    CCTGCAGATG GGATCCTGAT CCAGGGCAAC GATCTCAAGA TTGATGAGAG TTCTCTGACT
    GGGGAATCAG ACCATGTCAA GAAGTCCTTG GAGAGGGACC CCATGCTGCT CTCAGGGACC
    CATGTAATGG AAGGTTCTGG ACGAATGGTA GTGACGGCTG TGGGTATCAA CTCTCAGACT
    GGAATCATTT TTACCCTCTT GGGAGCCAGT GAGGGGGAAG AGGAAGAGAA GAAGAAGAAA
    GGTAAAAAAC AAGGAGTCCC TGAAAATCGC AACAAAGCAA AGACCCAAGA TGGAGTGGCC
    TTGGAAATCC AGCCACTCAA CAGCCAGGAG GGGATCGACA GTGAGGAAAA GGAGAAGAAG
    GCCGCCAAGC TGCCCAAAAA GGAGAAGTCC GTGCTGCAGG GCAAGCTGAC CCGCCTGGCT
    GTCCAGATTG GGAAAGCTGG TCTGATCATG TCTGCAATCA CAGTTCTCAT CCTGATTCTG
    TACTTCGTGA TCGACAACTT TGTGATCCAG CGCAGACCGT GGCTGGCCGA GTGTACCCCC
    ATCTACGTCC AGTACTTTGT CAAGTTCTTC ATCATCGGCG TCACGGTGCT GGTGGTGGCT
    GTGCCCGAGG GGCTGCCCCT GGCCGTCACC ATCTCACTGG CTTACTCTGT GAAGAAAATG
    ATGAAGGACA ACAACCTGGT GCGGCACTTG GACGCGTGTG AGACCATGGG CAACGCCACA
    GCCATCTGCT CGGACAAGAC GGGCACGCTG ACCATGAACC GCATGTCTGT GGTGCAGGCT
    TACATTGGAG ACACTCGTTA CCATCAGATC CCAAGCCCTG ATGACCTTGT GCCCAAGGTC
    CTGGACCTTA TTGTCAATGG CATTTCCATC AACAGTGCCT ACACCTCTAA GATTCTGCCT
    CCGGAGAAGG AGGGCGGCCT GCCCCGGCAG GTGGGCAACA AGACCGAGTG CGCCCTGCTG
    GGCTTCGTCT CGGACCTGAA GCAGGATTAC CACGCCGTGC GCAGCGAGGT GCCCGAGGAG
    AAGCTCTACA AAGTGTACAC CTTCAACTCG GTGCGCAAGT CCATGAGCAC GGTCATCGAG
    AAGCCGGGCG GCGGCTACCG CATGTACAGC AAGGGCGCCT CCGAGATCAT CCTGCGCAAG
    TGTAATCGAA TCCTGGACAA AAAGGGGGAG GCCGTGCCAT TCAAGAATAA GGACCGAGAT
    GAGATGGTCC GCACCGTCAT CGAGCCCATG GCCTGTGAGG GGCTGCGGAC ACTCTGCATC
    GCTTACCGGG ATTTCAACGA TGGAGAGCCC CCGTGGGACA ACGAGAGCGA GATCCTCACT
    GAGTTGACCT GTATCGCAGT GGTGGGCATC GAGGACCCCG TGCGCCCGGA GGTGCCAGAA
    GCTATTGCCA AATGCAAGCG AGCTGGCATC ACTGTCAGGA TGGTGACAGG GGACAACATC
    AACACAGCCC GTGCCATCGC CACCAAGTGT GGCATCGTGA CTCCCGGGGA TGACTTTCTG
    TGCTTAGAAG GCAAAGAATT CAATCGCCTC ATCCGAAACG AGAAGGGCGA GGTAGAGCAG
    GAAAAGCTGG ACAAGATCTG GCCTAAGCTC CGAGTGCTGG CGCGGTCTTC CCCCACTGAC
    AAGCACACGC TGGTGAAAGG CATCATCGAC AGCACTGTCG GCGACCAGCG GCAGGTGGTG
    GCCGTCACCG GCGACGGCAC CAACGACGGC CCGGCTCTGA AGAAAGCAGA CGTTGGTTTT
    GCCATGGGTA TTGCAGGCAC AGATGTGGCA AAGGAGGCCT CCGACATCAT CCTAACAGAC
    GACAACTTCA CCAGCATCGT GAAGGCGGTG ATGTGGGGAC GGAACGTCTA CGACAGCATC
    TCCAAGTTCC TGCAGTTCCA GCTCACCGTC AACGTGGTGG CCGTGATCGT GGCCTTCACT
    GGAGCCTGCA TCACCCAGGA TTCCCCACTG AAGGCCGTGC AGATGTTGTG GGTTAATCTA
    ATCATGGACA CCTTTGCCTC GCTGGCCCTG GCCACAGAGC CTCCTACAGA CTCTCTACTG
    AAGCGCCGGC CCTACGGCCG CAATAAGCCT CTAATCTCGC GCACCATGAT GAAGAACATC
    CTGGGTCACG CCGTCTATCA GCTCACCGTC ATCTTTTTCC TTGTGTTTGC GGGTGAGAAA
    TTCTTTGACA TTGACAGCGG GAGGAGAGCG CCTCTGCATT CTCCGCCCAG CCAGCACTAC
    ACCATTATTT TCAACACCTT CGTGCTCATG CAGCTCTTCA ACGAAATCAA CTCCCGCAAG
    ATCCACGGGG AGAGGAATGT CTTCTCGGGC ATCTTCCGCA ATCTCATCTT CTGCTCAGTG
    GTGTTGGGCA CATTCATCAG CCAGATCATC ATCGTGGAAT TCGGGGGCAA ACCCTTCAGT
    TGCACGAAGC TCACCCTGTC CCAGTGGTTC TGGTGCCTCT TCATTGGTAT TGGAGAGCTG
    CTGTGGGGCC AGGTCATTTC CACGATACCT ACCCAATCCC TGAAGTTCCT GAAGGAGGCT
    GGGCATGGCA CCACCAAAGA GGAGATCACC AAGGATGCTG AGGGGCTGGA CGAAATCGAC
    CATGCAGAGA TGGAGCTGCG CCGAGGCCAG ATCCTCTGGT TCCGGGGCCT GAACCGTATC
    CAGACTCAGA TCGACGTAAT TAACACATTC CAGACGGGAG CCTCTTTTAA GGGAGTCCTA
    AAGCGCCAGA CCATGGGTCA ACACCTTGAT GTAAAACATG TTCCTAGCTC ATCCTATATC
    AAAGTGGTCA AAGCGTTCCA TAGTTCCCTC CACGAAAGCA TTCAGAAACC CAAGAACCAA
    AACTCCATCC ACAACTTCAT GACCCACCCT GAATTCACCA TAGATGAGGA GGGGCCACGA
    ACACCACTCC TGGATGAGCA AGAAGAGGAG ATTTTTGAAA AGGTGTCTAA GCCTGGGACT
    AAGACGTCCT CACTGGATGG TGAGGTCACT CCACAAACCA ACAAAAACAA CAATACGGTG
    GATTGCTGCC AAGTACAAAT TGTTGCTTCC CACTCGGACA GCCCTCTACA CAGCCTGGAG
    ACGTCCGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831819.1
    CDS940..4671
    Translation

    Target ORF information:

    RefSeq Version XM_024976051.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ATPase plasma membrane Ca2+ transporting 4 (ATP2B4), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976051.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGACGAACC CAACAGAACA CACCTTGCCG TCCAACTCGA TACTTGAGAG CCGTGAAGGG 
    GAGTTTGGCT GCACAGTGAT GGACCTGAGG AAGCTGATGG AGCTGCGCTC AAGTGATGCA
    ATAGACCAGA TCAACGTCCA CTACGGAGGA GTGATGAACC TCTGCAGTAG ACTGAAAACC
    AACCCTGTGG AAGGCCTATC TGGGAACCCT GCAGATCTGG AGAAACGTAA ACAAGTGTTT
    GGACAGAACT TGATTCCCCC CAAAAAGCCC AAGACCTTCT TAGAGTTAGT GTGGGAAGCC
    CTCCAGGATG TCACGCTCAT CATCCTGGAG ATTGCGGCCA TCATCTCCCT GGTCCTGTCC
    TTCTACCGCC CCCCGGGGGG AGAAAATGAA CAGTGTGGTC TAGCCGTCAC TAGCCCAGAG
    GACGAAGGGG AGGCGGAAGC TGGCTGGATT GAGGGTGCAG CCATCTTATT CTCGGTGATC
    ATCGTCGTGC TCGTGACCGC CTTCAATGAC TGGAGCAAGG AGAAGCAATT CCGGGGGCTG
    CAGAACCGCA TCGAAAAGGA GCAGAAATTC TCCGTTATCC GGAATGGCCA CATCATTCAG
    CTCCCTGTGG CTGAGATTGT GGTGGGCGAC ATTGCCCAAA TCAAATACGG TGACCTGCTG
    CCTGCAGATG GGATCCTGAT CCAGGGCAAC GATCTCAAGA TTGATGAGAG TTCTCTGACT
    GGGGAATCAG ACCATGTCAA GAAGTCCTTG GAGAGGGACC CCATGCTGCT CTCAGGGACC
    CATGTAATGG AAGGTTCTGG ACGAATGGTA GTGACGGCTG TGGGTATCAA CTCTCAGACT
    GGAATCATTT TTACCCTCTT GGGAGCCAGT GAGGGGGAAG AGGAAGAGAA GAAGAAGAAA
    GGTAAAAAAC AAGGAGTCCC TGAAAATCGC AACAAAGCAA AGACCCAAGA TGGAGTGGCC
    TTGGAAATCC AGCCACTCAA CAGCCAGGAG GGGATCGACA GTGAGGAAAA GGAGAAGAAG
    GCCGCCAAGC TGCCCAAAAA GGAGAAGTCC GTGCTGCAGG GCAAGCTGAC CCGCCTGGCT
    GTCCAGATTG GGAAAGCTGG TCTGATCATG TCTGCAATCA CAGTTCTCAT CCTGATTCTG
    TACTTCGTGA TCGACAACTT TGTGATCCAG CGCAGACCGT GGCTGGCCGA GTGTACCCCC
    ATCTACGTCC AGTACTTTGT CAAGTTCTTC ATCATCGGCG TCACGGTGCT GGTGGTGGCT
    GTGCCCGAGG GGCTGCCCCT GGCCGTCACC ATCTCACTGG CTTACTCTGT GAAGAAAATG
    ATGAAGGACA ACAACCTGGT GCGGCACTTG GACGCGTGTG AGACCATGGG CAACGCCACA
    GCCATCTGCT CGGACAAGAC GGGCACGCTG ACCATGAACC GCATGTCTGT GGTGCAGGCT
    TACATTGGAG ACACTCGTTA CCATCAGATC CCAAGCCCTG ATGACCTTGT GCCCAAGGTC
    CTGGACCTTA TTGTCAATGG CATTTCCATC AACAGTGCCT ACACCTCTAA GATTCTGCCT
    CCGGAGAAGG AGGGCGGCCT GCCCCGGCAG GTGGGCAACA AGACCGAGTG CGCCCTGCTG
    GGCTTCGTCT CGGACCTGAA GCAGGATTAC CACGCCGTGC GCAGCGAGGT GCCCGAGGAG
    AAGCTCTACA AAGTGTACAC CTTCAACTCG GTGCGCAAGT CCATGAGCAC GGTCATCGAG
    AAGCCGGGCG GCGGCTACCG CATGTACAGC AAGGGCGCCT CCGAGATCAT CCTGCGCAAG
    TGTAATCGAA TCCTGGACAA AAAGGGGGAG GCCGTGCCAT TCAAGAATAA GGACCGAGAT
    GAGATGGTCC GCACCGTCAT CGAGCCCATG GCCTGTGAGG GGCTGCGGAC ACTCTGCATC
    GCTTACCGGG ATTTCAACGA TGGAGAGCCC CCGTGGGACA ACGAGAGCGA GATCCTCACT
    GAGTTGACCT GTATCGCAGT GGTGGGCATC GAGGACCCCG TGCGCCCGGA GGTGCCAGAA
    GCTATTGCCA AATGCAAGCG AGCTGGCATC ACTGTCAGGA TGGTGACAGG GGACAACATC
    AACACAGCCC GTGCCATCGC CACCAAGTGT GGCATCGTGA CTCCCGGGGA TGACTTTCTG
    TGCTTAGAAG GCAAAGAATT CAATCGCCTC ATCCGAAACG AGAAGGGCGA GGTAGAGCAG
    GAAAAGCTGG ACAAGATCTG GCCTAAGCTC CGAGTGCTGG CGCGGTCTTC CCCCACTGAC
    AAGCACACGC TGGTGAAAGG CATCATCGAC AGCACTGTCG GCGACCAGCG GCAGGTGGTG
    GCCGTCACCG GCGACGGCAC CAACGACGGC CCGGCTCTGA AGAAAGCAGA CGTTGGTTTT
    GCCATGGGTA TTGCAGGCAC AGATGTGGCA AAGGAGGCCT CCGACATCAT CCTAACAGAC
    GACAACTTCA CCAGCATCGT GAAGGCGGTG ATGTGGGGAC GGAACGTCTA CGACAGCATC
    TCCAAGTTCC TGCAGTTCCA GCTCACCGTC AACGTGGTGG CCGTGATCGT GGCCTTCACT
    GGAGCCTGCA TCACCCAGGA TTCCCCACTG AAGGCCGTGC AGATGTTGTG GGTTAATCTA
    ATCATGGACA CCTTTGCCTC GCTGGCCCTG GCCACAGAGC CTCCTACAGA CTCTCTACTG
    AAGCGCCGGC CCTACGGCCG CAATAAGCCT CTAATCTCGC GCACCATGAT GAAGAACATC
    CTGGGTCACG CCGTCTATCA GCTCACCGTC ATCTTTTTCC TTGTGTTTGC GGGTGAGAAA
    TTCTTTGACA TTGACAGCGG GAGGAGAGCG CCTCTGCATT CTCCGCCCAG CCAGCACTAC
    ACCATTATTT TCAACACCTT CGTGCTCATG CAGCTCTTCA ACGAAATCAA CTCCCGCAAG
    ATCCACGGGG AGAGGAATGT CTTCTCGGGC ATCTTCCGCA ATCTCATCTT CTGCTCAGTG
    GTGTTGGGCA CATTCATCAG CCAGATCATC ATCGTGGAAT TCGGGGGCAA ACCCTTCAGT
    TGCACGAAGC TCACCCTGTC CCAGTGGTTC TGGTGCCTCT TCATTGGTAT TGGAGAGCTG
    CTGTGGGGCC AGGTCATTTC CACGATACCT ACCCAATCCC TGAAGTTCCT GAAGGAGGCT
    GGGCATGGCA CCACCAAAGA GGAGATCACC AAGGATGCTG AGGGGCTGGA CGAAATCGAC
    CATGCAGAGA TGGAGCTGCG CCGAGGCCAG ATCCTCTGGT TCCGGGGCCT GAACCGTATC
    CAGACTCAGA TCGACGTAAT TAACACATTC CAGACGGGAG CCTCTTTTAA GGGAGTCCTA
    AAGCGCCAGA CCATGGGTCA ACACCTTGAT GTAAAACATG TTCCTAGCTC ATCCTATATC
    AAAGTGGTCA AAGCGTTCCA TAGTTCCCTC CACGAAAGCA TTCAGAAACC CAAGAACCAA
    AACTCCATCC ACAACTTCAT GACCCACCCT GAATTCACCA TAGATGAGGA GGGGCCACGA
    ACACCACTCC TGGATGAGCA AGAAGAGGAG ATTTTTGAAA AGGTGTCTAA GCCTGGGACT
    AAGACGTCCT CACTGGATGG TGAGGTCACT CCACAAACCA ACAAAAACAA CAATACGGTG
    GATTGCTGCC AAGTACAAAT TGTTGCTTCC CACTCGGACA GCCCTCTACA CAGCCTGGAG
    ACGTCCGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa39523
    Clone ID Related Accession (Same CDS sequence) NM_001172594.1
    Accession Version NM_001172594.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3480bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-21
    Organism Bos taurus(cattle)
    Product plasma membrane calcium-transporting ATPase 4
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from GU353069.1. On or before Jun 29, 2010 this sequence version replaced XM_002693900.1, XM_588082.3. ##Evidence-Data-START## Transcript exon combination :: GU353069.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145415, SAMN03145419 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGACGAACC CAACAGAACA CACCTTGCCG TCCAACTCGA TACTTGAGAG CCGTGAAGGG 
    GAGTTTGGCT GCACAGTGAT GGACCTGAGG AAGCTGATGG AGCTGCGCTC AAGTGATGCA
    ATAGACCAGA TCAACGTCCA CTACGGAGGA GTGATGAACC TCTGCAGTAG ACTGAAAACC
    AACCCTGTGG AAGGCCTATC TGGGAACCCT GCAGATCTGG AGAAACGTAA ACAAGTGTTT
    GGACAGAACT TGATTCCCCC CAAAAAGCCC AAGACCTTCT TAGAGTTAGT GTGGGAAGCC
    CTCCAGGATG TCACGCTCAT CATCCTGGAG ATTGCGGCCA TCATCTCCCT GGTCCTGTCC
    TTCTACCGCC CCCCGGGGGG AGAAAATGAA CAGTGTGGTC TAGCCGTCAC TAGCCCAGAG
    GACGAAGGGG AGGCGGAAGC TGGCTGGATT GAGGGTGCAG CCATCTTATT CTCGGTGATC
    ATCGTCGTGC TCGTGACCGC CTTCAATGAC TGGAGCAAGG AGAAGCAATT CCGGGGGCCG
    CAGAACCGCA TCGAAAAGGA GCAGAAATTC TCCGTTATCC GGAATGGCCA CATCATTCAG
    CTCCCTGTGG CTGAGATTGT GGTGGGCGAC ATTGCCCAAA TCAAATACGG TGACCTGCTG
    CCTGCAGATG GGATCCTGAT CCAGGGCAAC GATCTCAAGA TTGATGAGAG TTCTCTGACT
    GGGGAATCAG ACCATGTCAA GAAGTCCTTG GAGAGGGACC CCATGCTGCT CTCAGGGACC
    CATGTAATGG AAGGTTCTGG ACGAATGGTA GTGACGGCTG TGGGTATCAA CTCTCAGACT
    GGAATCATTT TTACCCTCTT GGGAGCCAGT GAGGGGGAAG AGGAAGAGAA GAAGAAGAAA
    GCAAAGACCC AAGATGGAGT GGCCTTGGAA ATCCAGCCAC TCAACAGCCA GGAGGGGATC
    GACAGTGAGG AAAAGGAGAA GAAGGCCGCC AAGCTGCCCA AAAAGGAGAA GTCCGTGCTG
    CAGGGCAAGC TGACCCGCCT GGCTGTCCAG ATTGGGAAAG CTGGTCTGAT CATGTCTGCA
    ATCACAGTTC TCATCCTGAT TCTGTACTTC GTGATCGACA ACTTTGTGAT CCAGCGCAGA
    CCGTGGCTGG CCGAGTGTAC CCCCATCTAC GTCCAGTACT TTGTCAAGTT CTTCATCATC
    GGCGTCACGG TGCTGGTGGT GGCTGTGCCC GAGGGGCTGC CCCTGGCCGT CACCATCTCA
    CTGGCTTACT CTGTGAAGAA AATGATGAAG GACAACAACC TGGTGCGGCA CTTGGACGCG
    TGTGAGACCA TGGGTAACGC CACAGCCATC TGCTCGGACA AGACGGGCAC GCTGACCATG
    AACCGCATGT CTGTGGTGCA GGCTTACATT GGAGACACTC GTTACCATCA GATCCCAAGC
    CCTGATGACC TTGTGCCCAA GGTCCTGGAC CTTATTGTCA ATGGCATTTC CATCAACAGT
    GCCTACACCT CTAAGATTCT GCCTCCGGAG AAGGAGGGCG GCCTGCCCCG GCAGGTGGGC
    AACAAGACCG AGTGCGCCCT GCTGGGCTTC GTCTCGGACC TGAAGCAGGA TTACCACGCC
    GTGCGCAGCG AGGTGCCCGA GGAGAAGCTC TACAAAGTGT ACACCTTCAA CTCGGTGCGC
    AAGTCCATGA GCACGGTCAT CGAGAAGCCG GGCGGCGGCT ACCGCATGTA CAGCAAGGGT
    GCCTCCGAGA TCATCCTGCG CAAGTGTAAT CGAATCCTGG ACAAAAAGGG GGAGGCCGTG
    CCATTCAAGA ATAAGGACCG AGATGAGATG GTCCGCACCG TCATCGAGCC CATGGCCTGT
    GAGGGGCTGC GGACACTCTG CATCGCTTAC CGGGATTTCA ACGATGGAGA GCCCCCGTGG
    GACAACGAGA GCGAGATCCT CACTGAGTTG ACCTGTATCG CAGTGGTGGG CATCGAGGAC
    CCCGTGCGCC CGGAGGTGCC AGAAGCTATT GCCAAATGCA AGCGAGCTGG CATCACTGTC
    AGGATGGTGA CAGGGGACAA CATCAACACA GCCCGTGCCA TCGCCACCAA GTGTGGCATC
    GTGACTCCCG GGGATGACTT TCTGTGCTTA GAAGGCAAAG AATTCAATCG CCTCATCCGA
    AACGAGAAGG GCGAGGTAGA GCAGGAAAAG CTGGACAAGA TCTGGCCTAA GCTCCGAGTG
    CTGGCGCGGT CTTCCCCCAC TGACAAGCAC ACGCTGGTGA AAGGCATCAT CGACAGCACT
    GTCGGCGACC AGCGGCAGGT GGTGGCCGTC ACCGGCGACG GCACCAACGA CGGCCCGGCT
    CTGAAGAAAG CAGACGTTGG TTTTGCCATG GGTATTGCAG GCACAGATGT GGCAAAGGAG
    GCCTCCGACA TCATCCTAAC AGACGACAAC TTCACCAGCA TCGTGAAGGC GGTGATGTGG
    GGACGGAACG TCTACGACAG CATCTCCAAG TTCCTGCAGT TCCAGCTCAC CGTCAACGTG
    GTGGCCGTGA TCGTGGCCTT CACTGGAGCC TGCATCACCC AGGATTCCCC ACTGAAGGCC
    GTGCAGATGT TGTGGGTTAA TCTAATCATG GACACCTTTG CCTCGCTGGC CCTGGCCACA
    GAGCCTCCTA CAGACTCTCT ACTGAAGCGC CGGCCCTACG GCCGCAATAA GCCTCTAATC
    TCGCGCACCA TGATGAAGAA CATCCTGGGT CACGCCGTCT ATCAGCTCAC CGTCATCTTT
    TTCCTTGTGT TTGCGGGTGA GAAATTCTTT GACATTGACA GCGGGAGGAG AGCGCCTCTG
    CATTCTCCGC CCAGCCAGCA CTACACCATT ATTTTCAACA CCTTCGTGCT CATGCAGCTC
    TTCAACGAAA TCAACTCCCG CAAGATCCAC GGGGAGAGGA ATGTCTTCTC GGGCATCTTC
    CGCAATCTCA TCTTCTGCTC AGTGGTGTTG GGCACATTCA TCAGCCAGAT CATCATCGTG
    GAATTCGGGG GCAAACCCTT CAGTTGCACG AAGCTCACCC TGTCCCAGTG GTTCTGGTGC
    CTCTTCATTG GTATTGGAGA GCTGCTGTGG GGCCAGGTCA TTTCCACGAT ACCTACCCAA
    TCCCTGAAGT TCCTGAAGGA GGCTGGGCAT GGCACCACCA AAGAGGAGAT CACCAAGGAT
    GCTGAGGGGC TGGACGAAAT CGACCATGCA GAGATGGAGC TGCGCCGAGG CCAGATCCTC
    TGGTTCCGGG GCCTGAACCG TATCCAGACT CAGATCGACG TAATTAACAC ATTCCAGACG
    GGAGCCTCTT TTAAGGGAGT CCTAAAGCGC CAGACCATGG GTCAACACCT TGATGTAAAA
    CATGTTCCTA GCTCATCCTA TGTAACAGTT GCACCAGTCA AATCTCCCCC CACCACTTCT
    GTTGCTGCTG CTGTTTCATC TCCTACTCTG GGCAATCAAA GTGGTCAAAG CGTTCCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001166065.1
    CDS1..3480
    Translation

    Target ORF information:

    RefSeq Version NM_001172594.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ATPase plasma membrane Ca2+ transporting 4 (ATP2B4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001172594.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGACGAACC CAACAGAACA CACCTTGCCG TCCAACTCGA TACTTGAGAG CCGTGAAGGG 
    GAGTTTGGCT GCACAGTGAT GGACCTGAGG AAGCTGATGG AGCTGCGCTC AAGTGATGCA
    ATAGACCAGA TCAACGTCCA CTACGGAGGA GTGATGAACC TCTGCAGTAG ACTGAAAACC
    AACCCTGTGG AAGGCCTATC TGGGAACCCT GCAGATCTGG AGAAACGTAA ACAAGTGTTT
    GGACAGAACT TGATTCCCCC CAAAAAGCCC AAGACCTTCT TAGAGTTAGT GTGGGAAGCC
    CTCCAGGATG TCACGCTCAT CATCCTGGAG ATTGCGGCCA TCATCTCCCT GGTCCTGTCC
    TTCTACCGCC CCCCGGGGGG AGAAAATGAA CAGTGTGGTC TAGCCGTCAC TAGCCCAGAG
    GACGAAGGGG AGGCGGAAGC TGGCTGGATT GAGGGTGCAG CCATCTTATT CTCGGTGATC
    ATCGTCGTGC TCGTGACCGC CTTCAATGAC TGGAGCAAGG AGAAGCAATT CCGGGGGCCG
    CAGAACCGCA TCGAAAAGGA GCAGAAATTC TCCGTTATCC GGAATGGCCA CATCATTCAG
    CTCCCTGTGG CTGAGATTGT GGTGGGCGAC ATTGCCCAAA TCAAATACGG TGACCTGCTG
    CCTGCAGATG GGATCCTGAT CCAGGGCAAC GATCTCAAGA TTGATGAGAG TTCTCTGACT
    GGGGAATCAG ACCATGTCAA GAAGTCCTTG GAGAGGGACC CCATGCTGCT CTCAGGGACC
    CATGTAATGG AAGGTTCTGG ACGAATGGTA GTGACGGCTG TGGGTATCAA CTCTCAGACT
    GGAATCATTT TTACCCTCTT GGGAGCCAGT GAGGGGGAAG AGGAAGAGAA GAAGAAGAAA
    GCAAAGACCC AAGATGGAGT GGCCTTGGAA ATCCAGCCAC TCAACAGCCA GGAGGGGATC
    GACAGTGAGG AAAAGGAGAA GAAGGCCGCC AAGCTGCCCA AAAAGGAGAA GTCCGTGCTG
    CAGGGCAAGC TGACCCGCCT GGCTGTCCAG ATTGGGAAAG CTGGTCTGAT CATGTCTGCA
    ATCACAGTTC TCATCCTGAT TCTGTACTTC GTGATCGACA ACTTTGTGAT CCAGCGCAGA
    CCGTGGCTGG CCGAGTGTAC CCCCATCTAC GTCCAGTACT TTGTCAAGTT CTTCATCATC
    GGCGTCACGG TGCTGGTGGT GGCTGTGCCC GAGGGGCTGC CCCTGGCCGT CACCATCTCA
    CTGGCTTACT CTGTGAAGAA AATGATGAAG GACAACAACC TGGTGCGGCA CTTGGACGCG
    TGTGAGACCA TGGGTAACGC CACAGCCATC TGCTCGGACA AGACGGGCAC GCTGACCATG
    AACCGCATGT CTGTGGTGCA GGCTTACATT GGAGACACTC GTTACCATCA GATCCCAAGC
    CCTGATGACC TTGTGCCCAA GGTCCTGGAC CTTATTGTCA ATGGCATTTC CATCAACAGT
    GCCTACACCT CTAAGATTCT GCCTCCGGAG AAGGAGGGCG GCCTGCCCCG GCAGGTGGGC
    AACAAGACCG AGTGCGCCCT GCTGGGCTTC GTCTCGGACC TGAAGCAGGA TTACCACGCC
    GTGCGCAGCG AGGTGCCCGA GGAGAAGCTC TACAAAGTGT ACACCTTCAA CTCGGTGCGC
    AAGTCCATGA GCACGGTCAT CGAGAAGCCG GGCGGCGGCT ACCGCATGTA CAGCAAGGGT
    GCCTCCGAGA TCATCCTGCG CAAGTGTAAT CGAATCCTGG ACAAAAAGGG GGAGGCCGTG
    CCATTCAAGA ATAAGGACCG AGATGAGATG GTCCGCACCG TCATCGAGCC CATGGCCTGT
    GAGGGGCTGC GGACACTCTG CATCGCTTAC CGGGATTTCA ACGATGGAGA GCCCCCGTGG
    GACAACGAGA GCGAGATCCT CACTGAGTTG ACCTGTATCG CAGTGGTGGG CATCGAGGAC
    CCCGTGCGCC CGGAGGTGCC AGAAGCTATT GCCAAATGCA AGCGAGCTGG CATCACTGTC
    AGGATGGTGA CAGGGGACAA CATCAACACA GCCCGTGCCA TCGCCACCAA GTGTGGCATC
    GTGACTCCCG GGGATGACTT TCTGTGCTTA GAAGGCAAAG AATTCAATCG CCTCATCCGA
    AACGAGAAGG GCGAGGTAGA GCAGGAAAAG CTGGACAAGA TCTGGCCTAA GCTCCGAGTG
    CTGGCGCGGT CTTCCCCCAC TGACAAGCAC ACGCTGGTGA AAGGCATCAT CGACAGCACT
    GTCGGCGACC AGCGGCAGGT GGTGGCCGTC ACCGGCGACG GCACCAACGA CGGCCCGGCT
    CTGAAGAAAG CAGACGTTGG TTTTGCCATG GGTATTGCAG GCACAGATGT GGCAAAGGAG
    GCCTCCGACA TCATCCTAAC AGACGACAAC TTCACCAGCA TCGTGAAGGC GGTGATGTGG
    GGACGGAACG TCTACGACAG CATCTCCAAG TTCCTGCAGT TCCAGCTCAC CGTCAACGTG
    GTGGCCGTGA TCGTGGCCTT CACTGGAGCC TGCATCACCC AGGATTCCCC ACTGAAGGCC
    GTGCAGATGT TGTGGGTTAA TCTAATCATG GACACCTTTG CCTCGCTGGC CCTGGCCACA
    GAGCCTCCTA CAGACTCTCT ACTGAAGCGC CGGCCCTACG GCCGCAATAA GCCTCTAATC
    TCGCGCACCA TGATGAAGAA CATCCTGGGT CACGCCGTCT ATCAGCTCAC CGTCATCTTT
    TTCCTTGTGT TTGCGGGTGA GAAATTCTTT GACATTGACA GCGGGAGGAG AGCGCCTCTG
    CATTCTCCGC CCAGCCAGCA CTACACCATT ATTTTCAACA CCTTCGTGCT CATGCAGCTC
    TTCAACGAAA TCAACTCCCG CAAGATCCAC GGGGAGAGGA ATGTCTTCTC GGGCATCTTC
    CGCAATCTCA TCTTCTGCTC AGTGGTGTTG GGCACATTCA TCAGCCAGAT CATCATCGTG
    GAATTCGGGG GCAAACCCTT CAGTTGCACG AAGCTCACCC TGTCCCAGTG GTTCTGGTGC
    CTCTTCATTG GTATTGGAGA GCTGCTGTGG GGCCAGGTCA TTTCCACGAT ACCTACCCAA
    TCCCTGAAGT TCCTGAAGGA GGCTGGGCAT GGCACCACCA AAGAGGAGAT CACCAAGGAT
    GCTGAGGGGC TGGACGAAAT CGACCATGCA GAGATGGAGC TGCGCCGAGG CCAGATCCTC
    TGGTTCCGGG GCCTGAACCG TATCCAGACT CAGATCGACG TAATTAACAC ATTCCAGACG
    GGAGCCTCTT TTAAGGGAGT CCTAAAGCGC CAGACCATGG GTCAACACCT TGATGTAAAA
    CATGTTCCTA GCTCATCCTA TGTAACAGTT GCACCAGTCA AATCTCCCCC CACCACTTCT
    GTTGCTGCTG CTGTTTCATC TCCTACTCTG GGCAATCAAA GTGGTCAAAG CGTTCCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.