Atp2b4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Atp2b4
Entrez Gene ID	29600
Full Name	ATPase plasma membrane Ca2+ transporting 4
Synonyms	PMCA4
General protein information	Preferred Names ATPase plasma membrane Ca2+ transporting 4 Names plasma membrane calcium-transporting ATPase 4 ATPase, Ca++ transporting, plasma membrane 4 plasma membrane Ca2+-ATPases 4 plasma membrane calcium ATPase plasma membrane calcium pump
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 13 Map Location: 13q13
Summary	transports calcium; plays a role in regulation of calcium homeostasis and calcium-mediated signalling pathways [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_008769448.2	XP_008767670.1	plasma membrane calcium-transporting ATPase 4 isoform X2
XM_008769449.2	XP_008767671.1	plasma membrane calcium-transporting ATPase 4 isoform X3
NM_001005871.1	NP_001005871.1	plasma membrane calcium-transporting ATPase 4
XM_008769447.2	XP_008767669.1	plasma membrane calcium-transporting ATPase 4 isoform X1
XM_017598773.1	XP_017454262.1	plasma membrane calcium-transporting ATPase 4 isoform X5
XM_008769446.2	XP_008767668.1	plasma membrane calcium-transporting ATPase 4 isoform X1
XM_008769450.2	XP_008767672.1	plasma membrane calcium-transporting ATPase 4 isoform X4

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ATP2B4	XP_005640818.1
Arabidopsis thaliana (thale cress)	ACA10	NP_194719.1
	ATP2B4	NP_001028098.1
Mus musculus (house mouse)	Atp2b4	NP_998781.1
Rattus norvegicus (Norway rat)	Atp2b4	NP_001005871.1
Magnaporthe oryzae (rice blast fungus)	MGG_04890	XP_003712375.1
Schizosaccharomyces pombe (fission yeast)	SPAPB2B4.04c	NP_593890.1
Arabidopsis thaliana (thale cress)	ACA8	NP_200521.3
Oryza sativa (rice)	Os04g0605500	NP_001053795.1
Homo sapiens (human)	ATP2B4	NP_001675.3
Gallus gallus (chicken)	ATP2B4	XP_004948503.1
Arabidopsis thaliana (thale cress)	ACA9	NP_188755.2
Xenopus tropicalis (tropical clawed frog)	atp2b4	XP_004910734.1
Pan troglodytes (chimpanzee)	ATP2B4	XP_001156333.2
Bos taurus (cattle)	ATP2B4	NP_001166065.1
Danio rerio (zebrafish)	atp2b1b	NP_001129103.1
Neurospora crassa	NCU05154	XP_956652.1

Gene Ontology
Bibliography

Related articles in PubMed

Calcium efflux activity of plasma membrane Ca2+ ATPase-4 (PMCA4) mediates cell cycle progression in vascular smooth muscle cells.
Afroze T, Yang G, Khoshbin A, Tanwir M, Tabish T, Momen A, Husain M
The Journal of biological chemistry289(10)7221-31(2014 Mar)

Molecular profiling of synaptic vesicle docking sites reveals novel proteins but few differences between glutamatergic and GABAergic synapses.
Boyken J, Gr?nborg M, Riedel D, Urlaub H, Jahn R, Chua JJ
Neuron78(2)285-97(2013 Apr)

Interaction of plasma membrane Ca(2+)-ATPase isoform 4 with calcineurin A: implications for catecholamine secretion by PC12 cells.
Kosiorek M, Podszywalow-Bartnicka P, Zylinska L, Zablocki K, Pikula S
Biochemical and biophysical research communications411(2)235-40(2011 Jul)

Defining the membrane proteome of NK cells.
Ghosh D, Lippert D, Krokhin O, Cortens JP, Wilkins JA
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)

Mutants in the putative nucleotide-binding region of the plasma membrane Ca(2+)-pump. A reduction in activity due to slow dephosphorylation.
Adamo HP, Filoteo AG, Enyedi A, Penniston JT
The Journal of biological chemistry270(50)30111-4(1995 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This result indicates that the interaction of PMCA4 with calcineurin A plays a regulatory role in the signalling during catecholamine secretion.
Title: Interaction of plasma membrane Ca(2+)-ATPase isoform 4 with calcineurin A: implications for catecholamine secretion by PC12 cells.

The plasma membrane Ca(2+)-ATPase is a sensitive target of oxidative stress in primary neurons. Inactivation of this Ca(2+) transporter under prolonged oxidative stress could alter neuronal Ca(2+) signaling.
Title: Effects of paraquat-induced oxidative stress on the neuronal plasma membrane Ca(2+)-ATPase.

the nNOS-PMCA4b complex regulates contractility via cAMP and phosphorylation of both PLB and cTnI.
Title: Specific role of neuronal nitric-oxide synthase when tethered to the plasma membrane calcium pump in regulating the beta-adrenergic signal in the myocardium.

In the caput epididymidis, where PMCA4 is located in the epithelium splice variant 4b was more prominent.
Title: Expression and localization of PMCA4 in rat testis and epididymis.

plasma membrane Ca2+ pump 4b/CI binds to Ca2+/calmodulin-dependent membrane-associated kinase CASK
Title: Interaction of the plasma membrane Ca2+ pump 4b/CI with the Ca2+/calmodulin-dependent membrane-associated kinase CASK.

The following Atp2b4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Atp2b4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa58047	XM_017598773.1 Latest version!	Rattus norvegicus ATPase plasma membrane Ca2+ transporting 4 (Atp2b4), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ORa10575	NM_001005871.1 Latest version!	Rattus norvegicus ATPase plasma membrane Ca2+ transporting 4 (Atp2b4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
ORa10575	XM_008769449.2 Latest version!	Rattus norvegicus ATPase plasma membrane Ca2+ transporting 4 (Atp2b4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
ORa43325	XM_008769446.2 Latest version!	Rattus norvegicus ATPase plasma membrane Ca2+ transporting 4 (Atp2b4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
ORa43325	XM_008769447.2 Latest version!	Rattus norvegicus ATPase plasma membrane Ca2+ transporting 4 (Atp2b4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
ORa43326	XM_008769448.2 Latest version!	Rattus norvegicus ATPase plasma membrane Ca2+ transporting 4 (Atp2b4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
ORa43327	XM_008769450.2 Latest version!	Rattus norvegicus ATPase plasma membrane Ca2+ transporting 4 (Atp2b4), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Atp2b4 ( XM_017598773.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017454262.1 Rattus norvegicus ATPase plasma membrane Ca2+ transporting 4 (Atp2b4), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ORa58047
Clone ID Related Accession (Same CDS sequence)	XM_017598773.1
Accession Version	XM_017598773.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3726bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	plasma membrane calcium-transporting ATPase 4 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGAATC CATCAGGACA TAACTTGCCA GCCAACTCGG TAGCTGAGAG CTATGAAGGG 
GAGTTTGGCT GCACCTTAAT GGACCTGAGG AAGCTCATGG AGCTTCGTGC AGCTGATGCA 
GTGACCCAGA TCAGTGCCCA CTATGGAAGT GTACAAGAAA TCTGCGCAAG ACTGAAAACC 
TCCCCTGTAG AAGGTCTGTC TGGGAACCCT GCAGATTTGG AGAAGCGTAG ACTTGTTTTT 
GGGAAGAACA TGATACCTCC GAAAAAGCCC AAGACGTTCT TAGAATTAGT GTGGGAAGCC 
CTGCAGGATG TCACACTCAT CATCCTGGAG ATCGCAGCCA TCATATCCCT GGTCCTGTCC 
TTCTACCGGC CTCCTGGCGG GGAAAATGAA ATCTGTGGCC ACATTGTAAG TAACCCAGAA 
GAAGATGAGG AAGGAGAAAC TGGCTGGATT GAAGGGGCAG CCATTCTTGC CTCGGTGATC 
ATTGTGGTAT TCGTGACAGC CTTCAACGAC TGGAGCAAAG AGAAGCAGTT CCGGGGGCTG 
CAGAGTCGCA TTGAGCTTGA GCAAAAATTC TCCATTATCC GGAATGGTCA GCTTATACAA 
CTCCCAGTGG CTGAGATTGT GGTGGGAGAT ATTGCCCAGG TCAAATACGG TGACCTGCTG 
CCTGCCGACG GAATCCTAAT CCAGGGAAAT GATCTGAAGA TTGACGAGAG CTCTTTGACA 
GGAGAATCAG ATCATGTCAA GAAGACTCTG GACAAAGACC CCATGTTGCT CTCAGGGACT 
CATGTGATGG AAGGCTCTGG ACGGATGGTG GTGACTGCTG TGGGAATCAA TTCCCAGACT 
GGAATCATCT TCACCCTCTT AGGGGCTAAT GAGGAAGAAG ACGATGAGAA AAAGAAGAAA 
GGTAAAAAAC AAGGAGTCTC TGAAAATCGC AACAAAGCAA AAACCCAGGA TGGAGTGGCC 
TTGGAAATCC AGCCACTCAA CAGCCAGGAG GGGCTCGATA GTGAGGAAAA GGAGAAGAAA 
GCAAGCAAGG GACCCAAGAA GGAGAAGTCG GTCCTGCAGG GCAAGCTCAC ACGCCTGGCT 
GTGCAGATCG GGAAGGCAGG TCTGATCATG TCTATCCTCA CAGTCCTCAT CCTGATTCTA 
TACTTCGTGG TTGACAATTT CGTGATACAG CGCCGGGCAT GGCTTCCTGA GTGCACTCCT 
GTGTACATCC AGTATTTTGT CAAGTTCTTC ATCATCGGAG TCACTGTACT GGTAGTGGCT 
GTGCCAGAGG GGCTGCCACT GGCTGTCACC ATCTCGTTGG CCTACTCTGT AAAGAAAATG 
ATGAAGGACA ACAACTTGGT CCGGCACTTG GACGCCTGTG AGACGATGGG CAACGCCACA 
GCCATCTGCT CTGATAAGAC GGGCACGCTG ACCATGAACC GCATGACCGT GGTACAAGCT 
TACATTGGGG GCACTCACTA CCGCCAGATC CCAAAACCTG ACGACCTCCC ACCCAACGTT 
CTGGACCTCA TAGTCAACAG CATCTGTATC AACTCTGCTT ACACTTCTAA GATTCTGCCT 
CCAGAGAAAG AGGGAGGCCT CCCCCGGCAA GTAGGCAACA AGACAGAGTG TGGACTGCTG 
GGCTTTGTCA CAGACCTGAA GCAGGACTAC CAAGCAGTTC GCAGTGAGAT GCCCGAGGAG 
AAGCTCTTCA AGGTGTACAC CTTCAACTCA GTGCGCAAGT CCATGAGCAC CGTCATCAGG 
AAGCCGGAAG GGGGCTTCCG CGTGTTCAGC AAGGGCGCCT CCGAGATAAT GCTGCGCAAG 
TGTGATCGGA TCCTGAACAA GGAAGGAGGA ATTGTACCAT TCAAAACTAA GGACCGAGAC 
AATATGGTAC GCAACGTCAT TGAGCCCATG GCCAGTGAAG GACTCCGGAC CATCGGCATA 
GCTTACCGAG ATTTTGATGG TGAAGAGCCC TCGTGGGAAA ATGAGAATGA GATCTTCACT 
GGGCTGGTCT GCATTGCAGT GGTGGGCATT GAGGACCCTG TGCGCCCAGA GGTACCAGAT 
GCCATTAACA AGTGCAAACG GGCTGGCATT ACTGTCAGGA TGGTGACAGG TGACAACGTC 
AACACAGCCA GGGCCATCGC CACCAAATGT GGCATCTTGA CTCCTGGTGA TGACTTCCTG 
TGTCTGGAAG GGAAAGAATT CAACAGACTC ATACGCAATG AGAAGGGCGA GGTCGAACAA 
GAAAAGCTGG ACAAGGTCTG GCCCAGGCTT CGGGTACTGG CACGATCTTC TCCCACAGAC 
AAGCACACAT TGGTGAAAGG TATCATTGAC AGCAATATTG GGGAACAGAG GCAGGTGGTG 
GCTGTCACCG GTGATGGAAC CAATGATGGA CCGGCTCTAA AGAAAGCAGA TGTCGGTTTT 
GCTATGGGCA TTGCAGGCAC AGATGTGGCT AAGGAGGCAT CAGACATCAT CCTGACTGAT 
GACAACTTCA CCAGCATTGT GAAGGCAGTG ATGTGGGGCC GGAACGTATA CGACAGCATT 
TCCAAGTTCC TGCAGTTCCA GCTCACTGTC AACGTGGTGG CCGTGATTGT GGCTTTCTCT 
GGAGCCTGCA TCACTCAGGA TTCCCCACTG AAAGCGGTGC AGATGCTGTG GGTGAACCTG 
ATCATGGACA CGTTTGCTTC TCTGGCCCTG GCCACAGAGC CCCCGACCGA TTCCCTGCTG 
AGGCGTCGCC CCTATGGGCG AAACAAGCCC CTGATCTCTC GCACAATGAT GAAGAACATC 
CTGGGCCACG CGGTGTATCA GCTCGGAATC GTCTTCCTCC TCGTCTTTGC TGGCGACAAA 
CTGTTTGACA TTGACAGTGG GAGGAAGGCG CCTCTCAATT CACCACCCAG CCAGCACTAC 
ACCATTGTTT TCAACACGTT TGTGCTGATG CAACTCTTCA ACGAGATCAA CTCTCGGAAG 
ATCCATGGCG AGAAGAATGT CTTTGCAGGT GTCTACCGCA ACATCATTTT CTGCTCTGTT 
GTTTTAGGCA CGTTCTTCTG CCAGATCTTG ATCGTGGAAG TGGGAGGTAA GCCCTTCAGC 
TGCACAAACC TCACCATGGA GCAGTGGATG TGGTGTCTCT TTATAGGGAT CGGAGAACTT 
TTGTGGGGCC AGGTCATCTC TGCAATACCT ACCAAGTCTC TAAAGTTCCT GAAAGAGGCG 
GGCCATGGCA GTGACAAAGA GGAGATCAGC AAGGACGCTG AGGGGCTGGA AGAGATCGAC 
CATGCCGAGA TGGAGCTTCG CCGAGGCCAG ATCCTCTGGG TCCGTGGCCT GAACCGGATT 
CAGACTCAGA TTGAAGTAAT TAACAAATTC CAGACGGGAG CCTCTTTTAA GGGAGTCCTA 
AGGCGACAGA ACTTGAGTCA ACAACTTGAT GTAAAACTGG TTCCTAGCTC CTATTCAGAA 
GCAATCAGAG TGGTCAAAGT ATTTCATAGT TTCCGAGACG TCATTCATAA ATCCAAGAAC 
CAGGTCTCCA TCCACAGCTT CATGACCCAA CCTGAGTACG CCGCAGACGA TGAAATGTCA 
CAGTCCTTCC TGAATCAAGA GGAAAGTCCT TCGTTGGCTT CTAAGTCGCG GATTACCAAG 
CGCCTGTCGG ATGCTGAAAC TGTTTCACAG AACAACACGA ATAACAATGC CGTGGACTGC 
CACCAAGTGC AAATCGTTGC CTCCCATCCC AACAGCCCTC TACAAAGCCA GGAGACACCG 
GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017454262.1
CDS	547..4272
Translation	MTNPSGHNLPANSVAESYEGEFGCTLMDLRKLMELRAADAVTQISAHYGSVQEICARLKTSPVEGLSGNPADLEKRRLVFGKNMIPPKKPKTFLELVWEALQDVTLIILEIAAIISLVLSFYRPPGGENEICGHIVSNPEEDEEGETGWIEGAAILASVIIVVFVTAFNDWSKEKQFRGLQSRIELEQKFSIIRNGQLIQLPVAEIVVGDIAQVKYGDLLPADGILIQGNDLKIDESSLTGESDHVKKTLDKDPMLLSGTHVMEGSGRMVVTAVGINSQTGIIFTLLGANEEEDDEKKKKGKKQGVSENRNKAKTQDGVALEIQPLNSQEGLDSEEKEKKASKGPKKEKSVLQGKLTRLAVQIGKAGLIMSILTVLILILYFVVDNFVIQRRAWLPECTPVYIQYFVKFFIIGVTVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTMNRMTVVQAYIGGTHYRQIPKPDDLPPNVLDLIVNSICINSAYTSKILPPEKEGGLPRQVGNKTECGLLGFVTDLKQDYQAVRSEMPEEKLFKVYTFNSVRKSMSTVIRKPEGGFRVFSKGASEIMLRKCDRILNKEGGIVPFKTKDRDNMVRNVIEPMASEGLRTIGIAYRDFDGEEPSWENENEIFTGLVCIAVVGIEDPVRPEVPDAINKCKRAGITVRMVTGDNVNTARAIATKCGILTPGDDFLCLEGKEFNRLIRNEKGEVEQEKLDKVWPRLRVLARSSPTDKHTLVKGIIDSNIGEQRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFTSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVIVAFSGACITQDSPLKAVQMLWVNLIMDTFASLALATEPPTDSLLRRRPYGRNKPLISRTMMKNILGHAVYQLGIVFLLVFAGDKLFDIDSGRKAPLNSPPSQHYTIVFNTFVLMQLFNEINSRKIHGEKNVFAGVYRNIIFCSVVLGTFFCQILIVEVGGKPFSCTNLTMEQWMWCLFIGIGELLWGQVISAIPTKSLKFLKEAGHGSDKEEISKDAEGLEEIDHAEMELRRGQILWVRGLNRIQTQIEVINKFQTGASFKGVLRRQNLSQQLDVKLVPSSYSEAIRVVKVFHSFRDVIHKSKNQVSIHSFMTQPEYAADDEMSQSFLNQEESPSLASKSRITKRLSDAETVSQNNTNNNAVDCHQVQIVASHPNSPLQSQETPV

Target ORF information:

RefSeq Version	XM_017598773.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ATPase plasma membrane Ca2+ transporting 4 (Atp2b4), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017598773.1

ORF Insert Sequence:

ATGACGAATC CATCAGGACA TAACTTGCCA GCCAACTCGG TAGCTGAGAG CTATGAAGGG 
GAGTTTGGCT GCACCTTAAT GGACCTGAGG AAGCTCATGG AGCTTCGTGC AGCTGATGCA 
GTGACCCAGA TCAGTGCCCA CTATGGAAGT GTACAAGAAA TCTGCGCAAG ACTGAAAACC 
TCCCCTGTAG AAGGTCTGTC TGGGAACCCT GCAGATTTGG AGAAGCGTAG ACTTGTTTTT 
GGGAAGAACA TGATACCTCC GAAAAAGCCC AAGACGTTCT TAGAATTAGT GTGGGAAGCC 
CTGCAGGATG TCACACTCAT CATCCTGGAG ATCGCAGCCA TCATATCCCT GGTCCTGTCC 
TTCTACCGGC CTCCTGGCGG GGAAAATGAA ATCTGTGGCC ACATTGTAAG TAACCCAGAA 
GAAGATGAGG AAGGAGAAAC TGGCTGGATT GAAGGGGCAG CCATTCTTGC CTCGGTGATC 
ATTGTGGTAT TCGTGACAGC CTTCAACGAC TGGAGCAAAG AGAAGCAGTT CCGGGGGCTG 
CAGAGTCGCA TTGAGCTTGA GCAAAAATTC TCCATTATCC GGAATGGTCA GCTTATACAA 
CTCCCAGTGG CTGAGATTGT GGTGGGAGAT ATTGCCCAGG TCAAATACGG TGACCTGCTG 
CCTGCCGACG GAATCCTAAT CCAGGGAAAT GATCTGAAGA TTGACGAGAG CTCTTTGACA 
GGAGAATCAG ATCATGTCAA GAAGACTCTG GACAAAGACC CCATGTTGCT CTCAGGGACT 
CATGTGATGG AAGGCTCTGG ACGGATGGTG GTGACTGCTG TGGGAATCAA TTCCCAGACT 
GGAATCATCT TCACCCTCTT AGGGGCTAAT GAGGAAGAAG ACGATGAGAA AAAGAAGAAA 
GGTAAAAAAC AAGGAGTCTC TGAAAATCGC AACAAAGCAA AAACCCAGGA TGGAGTGGCC 
TTGGAAATCC AGCCACTCAA CAGCCAGGAG GGGCTCGATA GTGAGGAAAA GGAGAAGAAA 
GCAAGCAAGG GACCCAAGAA GGAGAAGTCG GTCCTGCAGG GCAAGCTCAC ACGCCTGGCT 
GTGCAGATCG GGAAGGCAGG TCTGATCATG TCTATCCTCA CAGTCCTCAT CCTGATTCTA 
TACTTCGTGG TTGACAATTT CGTGATACAG CGCCGGGCAT GGCTTCCTGA GTGCACTCCT 
GTGTACATCC AGTATTTTGT CAAGTTCTTC ATCATCGGAG TCACTGTACT GGTAGTGGCT 
GTGCCAGAGG GGCTGCCACT GGCTGTCACC ATCTCGTTGG CCTACTCTGT AAAGAAAATG 
ATGAAGGACA ACAACTTGGT CCGGCACTTG GACGCCTGTG AGACGATGGG CAACGCCACA 
GCCATCTGCT CTGATAAGAC GGGCACGCTG ACCATGAACC GCATGACCGT GGTACAAGCT 
TACATTGGGG GCACTCACTA CCGCCAGATC CCAAAACCTG ACGACCTCCC ACCCAACGTT 
CTGGACCTCA TAGTCAACAG CATCTGTATC AACTCTGCTT ACACTTCTAA GATTCTGCCT 
CCAGAGAAAG AGGGAGGCCT CCCCCGGCAA GTAGGCAACA AGACAGAGTG TGGACTGCTG 
GGCTTTGTCA CAGACCTGAA GCAGGACTAC CAAGCAGTTC GCAGTGAGAT GCCCGAGGAG 
AAGCTCTTCA AGGTGTACAC CTTCAACTCA GTGCGCAAGT CCATGAGCAC CGTCATCAGG 
AAGCCGGAAG GGGGCTTCCG CGTGTTCAGC AAGGGCGCCT CCGAGATAAT GCTGCGCAAG 
TGTGATCGGA TCCTGAACAA GGAAGGAGGA ATTGTACCAT TCAAAACTAA GGACCGAGAC 
AATATGGTAC GCAACGTCAT TGAGCCCATG GCCAGTGAAG GACTCCGGAC CATCGGCATA 
GCTTACCGAG ATTTTGATGG TGAAGAGCCC TCGTGGGAAA ATGAGAATGA GATCTTCACT 
GGGCTGGTCT GCATTGCAGT GGTGGGCATT GAGGACCCTG TGCGCCCAGA GGTACCAGAT 
GCCATTAACA AGTGCAAACG GGCTGGCATT ACTGTCAGGA TGGTGACAGG TGACAACGTC 
AACACAGCCA GGGCCATCGC CACCAAATGT GGCATCTTGA CTCCTGGTGA TGACTTCCTG 
TGTCTGGAAG GGAAAGAATT CAACAGACTC ATACGCAATG AGAAGGGCGA GGTCGAACAA 
GAAAAGCTGG ACAAGGTCTG GCCCAGGCTT CGGGTACTGG CACGATCTTC TCCCACAGAC 
AAGCACACAT TGGTGAAAGG TATCATTGAC AGCAATATTG GGGAACAGAG GCAGGTGGTG 
GCTGTCACCG GTGATGGAAC CAATGATGGA CCGGCTCTAA AGAAAGCAGA TGTCGGTTTT 
GCTATGGGCA TTGCAGGCAC AGATGTGGCT AAGGAGGCAT CAGACATCAT CCTGACTGAT 
GACAACTTCA CCAGCATTGT GAAGGCAGTG ATGTGGGGCC GGAACGTATA CGACAGCATT 
TCCAAGTTCC TGCAGTTCCA GCTCACTGTC AACGTGGTGG CCGTGATTGT GGCTTTCTCT 
GGAGCCTGCA TCACTCAGGA TTCCCCACTG AAAGCGGTGC AGATGCTGTG GGTGAACCTG 
ATCATGGACA CGTTTGCTTC TCTGGCCCTG GCCACAGAGC CCCCGACCGA TTCCCTGCTG 
AGGCGTCGCC CCTATGGGCG AAACAAGCCC CTGATCTCTC GCACAATGAT GAAGAACATC 
CTGGGCCACG CGGTGTATCA GCTCGGAATC GTCTTCCTCC TCGTCTTTGC TGGCGACAAA 
CTGTTTGACA TTGACAGTGG GAGGAAGGCG CCTCTCAATT CACCACCCAG CCAGCACTAC 
ACCATTGTTT TCAACACGTT TGTGCTGATG CAACTCTTCA ACGAGATCAA CTCTCGGAAG 
ATCCATGGCG AGAAGAATGT CTTTGCAGGT GTCTACCGCA ACATCATTTT CTGCTCTGTT 
GTTTTAGGCA CGTTCTTCTG CCAGATCTTG ATCGTGGAAG TGGGAGGTAA GCCCTTCAGC 
TGCACAAACC TCACCATGGA GCAGTGGATG TGGTGTCTCT TTATAGGGAT CGGAGAACTT 
TTGTGGGGCC AGGTCATCTC TGCAATACCT ACCAAGTCTC TAAAGTTCCT GAAAGAGGCG 
GGCCATGGCA GTGACAAAGA GGAGATCAGC AAGGACGCTG AGGGGCTGGA AGAGATCGAC 
CATGCCGAGA TGGAGCTTCG CCGAGGCCAG ATCCTCTGGG TCCGTGGCCT GAACCGGATT 
CAGACTCAGA TTGAAGTAAT TAACAAATTC CAGACGGGAG CCTCTTTTAA GGGAGTCCTA 
AGGCGACAGA ACTTGAGTCA ACAACTTGAT GTAAAACTGG TTCCTAGCTC CTATTCAGAA 
GCAATCAGAG TGGTCAAAGT ATTTCATAGT TTCCGAGACG TCATTCATAA ATCCAAGAAC 
CAGGTCTCCA TCCACAGCTT CATGACCCAA CCTGAGTACG CCGCAGACGA TGAAATGTCA 
CAGTCCTTCC TGAATCAAGA GGAAAGTCCT TCGTTGGCTT CTAAGTCGCG GATTACCAAG 
CGCCTGTCGG ATGCTGAAAC TGTTTCACAG AACAACACGA ATAACAATGC CGTGGACTGC 
CACCAAGTGC AAATCGTTGC CTCCCATCCC AACAGCCCTC TACAAAGCCA GGAGACACCG 
GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Atp2b4 ( NM_001005871.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001005871.1 Rattus norvegicus ATPase plasma membrane Ca2+ transporting 4 (Atp2b4), mRNA.

Price & Availability↑

CloneID	ORa10575
Clone ID Related Accession (Same CDS sequence)	XM_008769449.2 , NM_001005871.1
Accession Version	NM_001005871.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3510bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Rattus norvegicus(Norway rat)
Product	plasma membrane calcium-transporting ATPase 4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U15408.1. On Oct 22, 2004 this sequence version replaced XM_341122.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U15408.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGACGAATC CATCAGGACA TAACTTGCCA GCCAACTCGG TAGCTGAGAG CTATGAAGGG 
GAGTTTGGCT GCACCTTAAT GGACCTGAGG AAGCTCATGG AGCTTCGTGC AGCTGATGCA 
GTGACCCAGA TCAGTGCCCA CTATGGAAGT GTACAAGAAA TCTGCGCAAG ACTGAAAACC 
TCCCCTGTAG AAGGTCTGTC TGGGAACCCT GCAGATTTGG AGAAGCGTAG ACTTGTTTTT 
GGGAAGAACA TGATACCTCC GAAAAAGCCC AAGACGTTCT TAGAATTAGT GTGGGAAGCC 
CTGCAGGATG TCACACTCAT CATCCTGGAG ATCGCAGCCA TCATATCCCT GGTCCTGTCC 
TTCTACCGGC CTCCTGGCGG GGAAAATGAA ATCTGTGGCC ACATTGTAAG TAACCCAGAA 
GAAGATGAGG AAGGAGAAAC TGGCTGGATT GAAGGGGCAG CCATTCTTGC CTCGGTGATC 
ATTGTGGTAT TCGTGACAGC CTTCAACGAC TGGAGCAAAG AGAAGCAGTT CCGGGGGCTG 
CAGAGTCGCA TTGAGCTTGA GCAAAAATTC TCCATTATCC GGAATGGTCA GCTTATACAA 
CTCCCAGTGG CTGAGATTGT GGTGGGAGAT ATTGCCCAGG TCAAATACGG TGACCTGCTG 
CCTGCCGACG GAATCCTAAT CCAGGGAAAT GATCTGAAGA TTGACGAGAG CTCTTTGACA 
GGAGAATCAG ATCATGTCAA GAAGACTCTG GACAAAGACC CCATGTTGCT CTCAGGGACT 
CATGTGATGG AAGGCTCTGG ACGGATGGTG GTGACTGCTG TGGGAATCAA TTCCCAGACT 
GGAATCATCT TCACCCTCTT AGGGGCTAAT GAGGAAGAAG ACGATGAGAA AAAGAAGAAA 
GGTAAAAAAC AAGGAGTCTC TGAAAATCGC AACAAAGCAA AAACCCAGGA TGGAGTGGCC 
TTGGAAATCC AGCCACTCAA CAGCCAGGAG GGGCTCGATA GTGAGGAAAA GGAGAAGAAA 
GCAAGCAAGG GACCCAAGAA GGAGAAGTCG GTCCTGCAGG GCAAGCTCAC ACGCCTGGCT 
GTGCAGATCG GGAAGGCAGG TCTGATCATG TCTATCCTCA CAGTCCTCAT CCTGATTCTA 
TACTTCGTGG TTGACAATTT CGTGATACAG CGCCGGGCAT GGCTTCCTGA GTGCACTCCT 
GTGTACATCC AGTATTTTGT CAAGTTCTTC ATCATCGGAG TCACTGTACT GGTAGTGGCT 
GTGCCAGAGG GGCTGCCACT GGCTGTCACC ATCTCGTTGG CCTACTCTGT AAAGAAAATG 
ATGAAGGACA ACAACTTGGT CCGGCACTTG GACGCCTGTG AGACGATGGG CAACGCCACA 
GCCATCTGCT CTGATAAGAC GGGCACGCTG ACCATGAACC GCATGACCGT GGTACAAGCT 
TACATTGGGG GCACTCACTA CCGCCAGATC CCAAAACCTG ACGACCTCCC ACCCAACGTT 
CTGGACCTCA TAGTCAACAG CATCTGTATC AACTCTGCTT ACACTTCTAA GATTCTGCCT 
CCAGAGAAAG AGGGAGGCCT CCCCCGGCAA GTAGGCAACA AGACAGAGTG TGGACTGCTG 
GGCTTTGTCA CAGACCTGAA GCAGGACTAC CAAGCAGTTC GCAGTGAGAT GCCCGAGGAG 
AAGCTCTTCA AGGTGTACAC CTTCAACTCA GTGCGCAAGT CCATGAGCAC CGTCATCAGG 
AAGCCGGAAG GGGGCTTCCG CGTGTTCAGC AAGGGCGCCT CCGAGATAAT GCTGCGCAAG 
TGTGATCGGA TCCTGAACAA GGAAGGAGGA ATTGTACCAT TCAAAACTAA GGACCGAGAC 
AATATGGTAC GCAACGTCAT TGAGCCCATG GCCAGTGAAG GACTCCGGAC CATCGGCATA 
GCTTACCGAG ATTTTGATGG TGAAGAGCCC TCGTGGGAAA ATGAGAATGA GATCTTCACT 
GGGCTGGTCT GCATTGCAGT GGTGGGCATT GAGGACCCTG TGCGCCCAGA GGTACCAGAT 
GCCATTAACA AGTGCAAACG GGCTGGCATT ACTGTCAGGA TGGTGACAGG TGACAACGTC 
AACACAGCCA GGGCCATCGC CACCAAATGT GGCATCTTGA CTCCTGGTGA TGACTTCCTG 
TGTCTGGAAG GGAAAGAATT CAACAGACTC ATACGCAATG AGAAGGGCGA GGTCGAACAA 
GAAAAGCTGG ACAAGGTCTG GCCCAGGCTT CGGGTACTGG CACGATCTTC TCCCACAGAC 
AAGCACACAT TGGTGAAAGG TATCATTGAC AGCAATATTG GGGAACAGAG GCAGGTGGTG 
GCTGTCACCG GTGATGGAAC CAATGATGGA CCGGCTCTAA AGAAAGCAGA TGTCGGTTTT 
GCTATGGGCA TTGCAGGCAC AGATGTGGCT AAGGAGGCAT CAGACATCAT CCTGACTGAT 
GACAACTTCA CCAGCATTGT GAAGGCAGTG ATGTGGGGCC GGAACGTATA CGACAGCATT 
TCCAAGTTCC TGCAGTTCCA GCTCACTGTC AACGTGGTGG CCGTGATTGT GGCTTTCTCT 
GGAGCCTGCA TCACTCAGGA TTCCCCACTG AAAGCGGTGC AGATGCTGTG GGTGAACCTG 
ATCATGGACA CGTTTGCTTC TCTGGCCCTG GCCACAGAGC CCCCGACCGA TTCCCTGCTG 
AGGCGTCGCC CCTATGGGCG AAACAAGCCC CTGATCTCTC GCACAATGAT GAAGAACATC 
CTGGGCCACG CGGTGTATCA GCTCGGAATC GTCTTCCTCC TCGTCTTTGC TGGCGACAAA 
CTGTTTGACA TTGACAGTGG GAGGAAGGCG CCTCTCAATT CACCACCCAG CCAGCACTAC 
ACCATTGTTT TCAACACGTT TGTGCTGATG CAACTCTTCA ACGAGATCAA CTCTCGGAAG 
ATCCATGGCG AGAAGAATGT CTTTGCAGGT GTCTACCGCA ACATCATTTT CTGCTCTGTT 
GTTTTAGGCA CGTTCTTCTG CCAGATCTTG ATCGTGGAAG TGGGAGGTAA GCCCTTCAGC 
TGCACAAACC TCACCATGGA GCAGTGGATG TGGTGTCTCT TTATAGGGAT CGGAGAACTT 
TTGTGGGGCC AGGTCATCTC TGCAATACCT ACCAAGTCTC TAAAGTTCCT GAAAGAGGCG 
GGCCATGGCA GTGACAAAGA GGAGATCAGC AAGGACGCTG AGGGGCTGGA AGAGATCGAC 
CATGCCGAGA TGGAGCTTCG CCGAGGCCAG ATCCTCTGGG TCCGTGGCCT GAACCGGATT 
CAGACTCAGA TTGAAGTAAT TAACAAATTC CAGACGGGAG CCTCTTTTAA GGGAGTCCTA 
AGGCGACAGA ACTTGAGTCA ACAACTTGAT GTAAAACTGG TTCCTAGCTC CTATTCAGAA 
GCAGTTGCGT CAGTCAGAAC CTCCCCCTCC ACCTCTTCTG CCGTTACACC TCCTCCTGTG 
GGCAATCAGA GTGGTCAAAG TATTTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001005871.1
CDS	168..3677
Translation	MTNPSGHNLPANSVAESYEGEFGCTLMDLRKLMELRAADAVTQISAHYGSVQEICARLKTSPVEGLSGNPADLEKRRLVFGKNMIPPKKPKTFLELVWEALQDVTLIILEIAAIISLVLSFYRPPGGENEICGHIVSNPEEDEEGETGWIEGAAILASVIIVVFVTAFNDWSKEKQFRGLQSRIELEQKFSIIRNGQLIQLPVAEIVVGDIAQVKYGDLLPADGILIQGNDLKIDESSLTGESDHVKKTLDKDPMLLSGTHVMEGSGRMVVTAVGINSQTGIIFTLLGANEEEDDEKKKKGKKQGVSENRNKAKTQDGVALEIQPLNSQEGLDSEEKEKKASKGPKKEKSVLQGKLTRLAVQIGKAGLIMSILTVLILILYFVVDNFVIQRRAWLPECTPVYIQYFVKFFIIGVTVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTMNRMTVVQAYIGGTHYRQIPKPDDLPPNVLDLIVNSICINSAYTSKILPPEKEGGLPRQVGNKTECGLLGFVTDLKQDYQAVRSEMPEEKLFKVYTFNSVRKSMSTVIRKPEGGFRVFSKGASEIMLRKCDRILNKEGGIVPFKTKDRDNMVRNVIEPMASEGLRTIGIAYRDFDGEEPSWENENEIFTGLVCIAVVGIEDPVRPEVPDAINKCKRAGITVRMVTGDNVNTARAIATKCGILTPGDDFLCLEGKEFNRLIRNEKGEVEQEKLDKVWPRLRVLARSSPTDKHTLVKGIIDSNIGEQRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFTSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVIVAFSGACITQDSPLKAVQMLWVNLIMDTFASLALATEPPTDSLLRRRPYGRNKPLISRTMMKNILGHAVYQLGIVFLLVFAGDKLFDIDSGRKAPLNSPPSQHYTIVFNTFVLMQLFNEINSRKIHGEKNVFAGVYRNIIFCSVVLGTFFCQILIVEVGGKPFSCTNLTMEQWMWCLFIGIGELLWGQVISAIPTKSLKFLKEAGHGSDKEEISKDAEGLEEIDHAEMELRRGQILWVRGLNRIQTQIEVINKFQTGASFKGVLRRQNLSQQLDVKLVPSSYSEAVASVRTSPSTSSAVTPPPVGNQSGQSIS

Target ORF information:

RefSeq Version	NM_001005871.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ATPase plasma membrane Ca2+ transporting 4 (Atp2b4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001005871.1

ORF Insert Sequence:

ATGACGAATC CATCAGGACA TAACTTGCCA GCCAACTCGG TAGCTGAGAG CTATGAAGGG 
GAGTTTGGCT GCACCTTAAT GGACCTGAGG AAGCTCATGG AGCTTCGTGC AGCTGATGCA 
GTGACCCAGA TCAGTGCCCA CTATGGAAGT GTACAAGAAA TCTGCGCAAG ACTGAAAACC 
TCCCCTGTAG AAGGTCTGTC TGGGAACCCT GCAGATTTGG AGAAGCGTAG ACTTGTTTTT 
GGGAAGAACA TGATACCTCC GAAAAAGCCC AAGACGTTCT TAGAATTAGT GTGGGAAGCC 
CTGCAGGATG TCACACTCAT CATCCTGGAG ATCGCAGCCA TCATATCCCT GGTCCTGTCC 
TTCTACCGGC CTCCTGGCGG GGAAAATGAA ATCTGTGGCC ACATTGTAAG TAACCCAGAA 
GAAGATGAGG AAGGAGAAAC TGGCTGGATT GAAGGGGCAG CCATTCTTGC CTCGGTGATC 
ATTGTGGTAT TCGTGACAGC CTTCAACGAC TGGAGCAAAG AGAAGCAGTT CCGGGGGCTG 
CAGAGTCGCA TTGAGCTTGA GCAAAAATTC TCCATTATCC GGAATGGTCA GCTTATACAA 
CTCCCAGTGG CTGAGATTGT GGTGGGAGAT ATTGCCCAGG TCAAATACGG TGACCTGCTG 
CCTGCCGACG GAATCCTAAT CCAGGGAAAT GATCTGAAGA TTGACGAGAG CTCTTTGACA 
GGAGAATCAG ATCATGTCAA GAAGACTCTG GACAAAGACC CCATGTTGCT CTCAGGGACT 
CATGTGATGG AAGGCTCTGG ACGGATGGTG GTGACTGCTG TGGGAATCAA TTCCCAGACT 
GGAATCATCT TCACCCTCTT AGGGGCTAAT GAGGAAGAAG ACGATGAGAA AAAGAAGAAA 
GGTAAAAAAC AAGGAGTCTC TGAAAATCGC AACAAAGCAA AAACCCAGGA TGGAGTGGCC 
TTGGAAATCC AGCCACTCAA CAGCCAGGAG GGGCTCGATA GTGAGGAAAA GGAGAAGAAA 
GCAAGCAAGG GACCCAAGAA GGAGAAGTCG GTCCTGCAGG GCAAGCTCAC ACGCCTGGCT 
GTGCAGATCG GGAAGGCAGG TCTGATCATG TCTATCCTCA CAGTCCTCAT CCTGATTCTA 
TACTTCGTGG TTGACAATTT CGTGATACAG CGCCGGGCAT GGCTTCCTGA GTGCACTCCT 
GTGTACATCC AGTATTTTGT CAAGTTCTTC ATCATCGGAG TCACTGTACT GGTAGTGGCT 
GTGCCAGAGG GGCTGCCACT GGCTGTCACC ATCTCGTTGG CCTACTCTGT AAAGAAAATG 
ATGAAGGACA ACAACTTGGT CCGGCACTTG GACGCCTGTG AGACGATGGG CAACGCCACA 
GCCATCTGCT CTGATAAGAC GGGCACGCTG ACCATGAACC GCATGACCGT GGTACAAGCT 
TACATTGGGG GCACTCACTA CCGCCAGATC CCAAAACCTG ACGACCTCCC ACCCAACGTT 
CTGGACCTCA TAGTCAACAG CATCTGTATC AACTCTGCTT ACACTTCTAA GATTCTGCCT 
CCAGAGAAAG AGGGAGGCCT CCCCCGGCAA GTAGGCAACA AGACAGAGTG TGGACTGCTG 
GGCTTTGTCA CAGACCTGAA GCAGGACTAC CAAGCAGTTC GCAGTGAGAT GCCCGAGGAG 
AAGCTCTTCA AGGTGTACAC CTTCAACTCA GTGCGCAAGT CCATGAGCAC CGTCATCAGG 
AAGCCGGAAG GGGGCTTCCG CGTGTTCAGC AAGGGCGCCT CCGAGATAAT GCTGCGCAAG 
TGTGATCGGA TCCTGAACAA GGAAGGAGGA ATTGTACCAT TCAAAACTAA GGACCGAGAC 
AATATGGTAC GCAACGTCAT TGAGCCCATG GCCAGTGAAG GACTCCGGAC CATCGGCATA 
GCTTACCGAG ATTTTGATGG TGAAGAGCCC TCGTGGGAAA ATGAGAATGA GATCTTCACT 
GGGCTGGTCT GCATTGCAGT GGTGGGCATT GAGGACCCTG TGCGCCCAGA GGTACCAGAT 
GCCATTAACA AGTGCAAACG GGCTGGCATT ACTGTCAGGA TGGTGACAGG TGACAACGTC 
AACACAGCCA GGGCCATCGC CACCAAATGT GGCATCTTGA CTCCTGGTGA TGACTTCCTG 
TGTCTGGAAG GGAAAGAATT CAACAGACTC ATACGCAATG AGAAGGGCGA GGTCGAACAA 
GAAAAGCTGG ACAAGGTCTG GCCCAGGCTT CGGGTACTGG CACGATCTTC TCCCACAGAC 
AAGCACACAT TGGTGAAAGG TATCATTGAC AGCAATATTG GGGAACAGAG GCAGGTGGTG 
GCTGTCACCG GTGATGGAAC CAATGATGGA CCGGCTCTAA AGAAAGCAGA TGTCGGTTTT 
GCTATGGGCA TTGCAGGCAC AGATGTGGCT AAGGAGGCAT CAGACATCAT CCTGACTGAT 
GACAACTTCA CCAGCATTGT GAAGGCAGTG ATGTGGGGCC GGAACGTATA CGACAGCATT 
TCCAAGTTCC TGCAGTTCCA GCTCACTGTC AACGTGGTGG CCGTGATTGT GGCTTTCTCT 
GGAGCCTGCA TCACTCAGGA TTCCCCACTG AAAGCGGTGC AGATGCTGTG GGTGAACCTG 
ATCATGGACA CGTTTGCTTC TCTGGCCCTG GCCACAGAGC CCCCGACCGA TTCCCTGCTG 
AGGCGTCGCC CCTATGGGCG AAACAAGCCC CTGATCTCTC GCACAATGAT GAAGAACATC 
CTGGGCCACG CGGTGTATCA GCTCGGAATC GTCTTCCTCC TCGTCTTTGC TGGCGACAAA 
CTGTTTGACA TTGACAGTGG GAGGAAGGCG CCTCTCAATT CACCACCCAG CCAGCACTAC 
ACCATTGTTT TCAACACGTT TGTGCTGATG CAACTCTTCA ACGAGATCAA CTCTCGGAAG 
ATCCATGGCG AGAAGAATGT CTTTGCAGGT GTCTACCGCA ACATCATTTT CTGCTCTGTT 
GTTTTAGGCA CGTTCTTCTG CCAGATCTTG ATCGTGGAAG TGGGAGGTAA GCCCTTCAGC 
TGCACAAACC TCACCATGGA GCAGTGGATG TGGTGTCTCT TTATAGGGAT CGGAGAACTT 
TTGTGGGGCC AGGTCATCTC TGCAATACCT ACCAAGTCTC TAAAGTTCCT GAAAGAGGCG 
GGCCATGGCA GTGACAAAGA GGAGATCAGC AAGGACGCTG AGGGGCTGGA AGAGATCGAC 
CATGCCGAGA TGGAGCTTCG CCGAGGCCAG ATCCTCTGGG TCCGTGGCCT GAACCGGATT 
CAGACTCAGA TTGAAGTAAT TAACAAATTC CAGACGGGAG CCTCTTTTAA GGGAGTCCTA 
AGGCGACAGA ACTTGAGTCA ACAACTTGAT GTAAAACTGG TTCCTAGCTC CTATTCAGAA 
GCAGTTGCGT CAGTCAGAAC CTCCCCCTCC ACCTCTTCTG CCGTTACACC TCCTCCTGTG 
GGCAATCAGA GTGGTCAAAG TATTTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Atp2b4 ( XM_008769449.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008767671.1 Rattus norvegicus ATPase plasma membrane Ca2+ transporting 4 (Atp2b4), transcript variant X4, mRNA.

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CloneID	ORa10575
Clone ID Related Accession (Same CDS sequence)	XM_008769449.2 , NM_001005871.1
Accession Version	XM_008769449.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3510bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	plasma membrane calcium-transporting ATPase 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008769449.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGAATC CATCAGGACA TAACTTGCCA GCCAACTCGG TAGCTGAGAG CTATGAAGGG 
GAGTTTGGCT GCACCTTAAT GGACCTGAGG AAGCTCATGG AGCTTCGTGC AGCTGATGCA 
GTGACCCAGA TCAGTGCCCA CTATGGAAGT GTACAAGAAA TCTGCGCAAG ACTGAAAACC 
TCCCCTGTAG AAGGTCTGTC TGGGAACCCT GCAGATTTGG AGAAGCGTAG ACTTGTTTTT 
GGGAAGAACA TGATACCTCC GAAAAAGCCC AAGACGTTCT TAGAATTAGT GTGGGAAGCC 
CTGCAGGATG TCACACTCAT CATCCTGGAG ATCGCAGCCA TCATATCCCT GGTCCTGTCC 
TTCTACCGGC CTCCTGGCGG GGAAAATGAA ATCTGTGGCC ACATTGTAAG TAACCCAGAA 
GAAGATGAGG AAGGAGAAAC TGGCTGGATT GAAGGGGCAG CCATTCTTGC CTCGGTGATC 
ATTGTGGTAT TCGTGACAGC CTTCAACGAC TGGAGCAAAG AGAAGCAGTT CCGGGGGCTG 
CAGAGTCGCA TTGAGCTTGA GCAAAAATTC TCCATTATCC GGAATGGTCA GCTTATACAA 
CTCCCAGTGG CTGAGATTGT GGTGGGAGAT ATTGCCCAGG TCAAATACGG TGACCTGCTG 
CCTGCCGACG GAATCCTAAT CCAGGGAAAT GATCTGAAGA TTGACGAGAG CTCTTTGACA 
GGAGAATCAG ATCATGTCAA GAAGACTCTG GACAAAGACC CCATGTTGCT CTCAGGGACT 
CATGTGATGG AAGGCTCTGG ACGGATGGTG GTGACTGCTG TGGGAATCAA TTCCCAGACT 
GGAATCATCT TCACCCTCTT AGGGGCTAAT GAGGAAGAAG ACGATGAGAA AAAGAAGAAA 
GGTAAAAAAC AAGGAGTCTC TGAAAATCGC AACAAAGCAA AAACCCAGGA TGGAGTGGCC 
TTGGAAATCC AGCCACTCAA CAGCCAGGAG GGGCTCGATA GTGAGGAAAA GGAGAAGAAA 
GCAAGCAAGG GACCCAAGAA GGAGAAGTCG GTCCTGCAGG GCAAGCTCAC ACGCCTGGCT 
GTGCAGATCG GGAAGGCAGG TCTGATCATG TCTATCCTCA CAGTCCTCAT CCTGATTCTA 
TACTTCGTGG TTGACAATTT CGTGATACAG CGCCGGGCAT GGCTTCCTGA GTGCACTCCT 
GTGTACATCC AGTATTTTGT CAAGTTCTTC ATCATCGGAG TCACTGTACT GGTAGTGGCT 
GTGCCAGAGG GGCTGCCACT GGCTGTCACC ATCTCGTTGG CCTACTCTGT AAAGAAAATG 
ATGAAGGACA ACAACTTGGT CCGGCACTTG GACGCCTGTG AGACGATGGG CAACGCCACA 
GCCATCTGCT CTGATAAGAC GGGCACGCTG ACCATGAACC GCATGACCGT GGTACAAGCT 
TACATTGGGG GCACTCACTA CCGCCAGATC CCAAAACCTG ACGACCTCCC ACCCAACGTT 
CTGGACCTCA TAGTCAACAG CATCTGTATC AACTCTGCTT ACACTTCTAA GATTCTGCCT 
CCAGAGAAAG AGGGAGGCCT CCCCCGGCAA GTAGGCAACA AGACAGAGTG TGGACTGCTG 
GGCTTTGTCA CAGACCTGAA GCAGGACTAC CAAGCAGTTC GCAGTGAGAT GCCCGAGGAG 
AAGCTCTTCA AGGTGTACAC CTTCAACTCA GTGCGCAAGT CCATGAGCAC CGTCATCAGG 
AAGCCGGAAG GGGGCTTCCG CGTGTTCAGC AAGGGCGCCT CCGAGATAAT GCTGCGCAAG 
TGTGATCGGA TCCTGAACAA GGAAGGAGGA ATTGTACCAT TCAAAACTAA GGACCGAGAC 
AATATGGTAC GCAACGTCAT TGAGCCCATG GCCAGTGAAG GACTCCGGAC CATCGGCATA 
GCTTACCGAG ATTTTGATGG TGAAGAGCCC TCGTGGGAAA ATGAGAATGA GATCTTCACT 
GGGCTGGTCT GCATTGCAGT GGTGGGCATT GAGGACCCTG TGCGCCCAGA GGTACCAGAT 
GCCATTAACA AGTGCAAACG GGCTGGCATT ACTGTCAGGA TGGTGACAGG TGACAACGTC 
AACACAGCCA GGGCCATCGC CACCAAATGT GGCATCTTGA CTCCTGGTGA TGACTTCCTG 
TGTCTGGAAG GGAAAGAATT CAACAGACTC ATACGCAATG AGAAGGGCGA GGTCGAACAA 
GAAAAGCTGG ACAAGGTCTG GCCCAGGCTT CGGGTACTGG CACGATCTTC TCCCACAGAC 
AAGCACACAT TGGTGAAAGG TATCATTGAC AGCAATATTG GGGAACAGAG GCAGGTGGTG 
GCTGTCACCG GTGATGGAAC CAATGATGGA CCGGCTCTAA AGAAAGCAGA TGTCGGTTTT 
GCTATGGGCA TTGCAGGCAC AGATGTGGCT AAGGAGGCAT CAGACATCAT CCTGACTGAT 
GACAACTTCA CCAGCATTGT GAAGGCAGTG ATGTGGGGCC GGAACGTATA CGACAGCATT 
TCCAAGTTCC TGCAGTTCCA GCTCACTGTC AACGTGGTGG CCGTGATTGT GGCTTTCTCT 
GGAGCCTGCA TCACTCAGGA TTCCCCACTG AAAGCGGTGC AGATGCTGTG GGTGAACCTG 
ATCATGGACA CGTTTGCTTC TCTGGCCCTG GCCACAGAGC CCCCGACCGA TTCCCTGCTG 
AGGCGTCGCC CCTATGGGCG AAACAAGCCC CTGATCTCTC GCACAATGAT GAAGAACATC 
CTGGGCCACG CGGTGTATCA GCTCGGAATC GTCTTCCTCC TCGTCTTTGC TGGCGACAAA 
CTGTTTGACA TTGACAGTGG GAGGAAGGCG CCTCTCAATT CACCACCCAG CCAGCACTAC 
ACCATTGTTT TCAACACGTT TGTGCTGATG CAACTCTTCA ACGAGATCAA CTCTCGGAAG 
ATCCATGGCG AGAAGAATGT CTTTGCAGGT GTCTACCGCA ACATCATTTT CTGCTCTGTT 
GTTTTAGGCA CGTTCTTCTG CCAGATCTTG ATCGTGGAAG TGGGAGGTAA GCCCTTCAGC 
TGCACAAACC TCACCATGGA GCAGTGGATG TGGTGTCTCT TTATAGGGAT CGGAGAACTT 
TTGTGGGGCC AGGTCATCTC TGCAATACCT ACCAAGTCTC TAAAGTTCCT GAAAGAGGCG 
GGCCATGGCA GTGACAAAGA GGAGATCAGC AAGGACGCTG AGGGGCTGGA AGAGATCGAC 
CATGCCGAGA TGGAGCTTCG CCGAGGCCAG ATCCTCTGGG TCCGTGGCCT GAACCGGATT 
CAGACTCAGA TTGAAGTAAT TAACAAATTC CAGACGGGAG CCTCTTTTAA GGGAGTCCTA 
AGGCGACAGA ACTTGAGTCA ACAACTTGAT GTAAAACTGG TTCCTAGCTC CTATTCAGAA 
GCAGTTGCGT CAGTCAGAAC CTCCCCCTCC ACCTCTTCTG CCGTTACACC TCCTCCTGTG 
GGCAATCAGA GTGGTCAAAG TATTTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008767671.1
CDS	547..4056
Translation	MTNPSGHNLPANSVAESYEGEFGCTLMDLRKLMELRAADAVTQISAHYGSVQEICARLKTSPVEGLSGNPADLEKRRLVFGKNMIPPKKPKTFLELVWEALQDVTLIILEIAAIISLVLSFYRPPGGENEICGHIVSNPEEDEEGETGWIEGAAILASVIIVVFVTAFNDWSKEKQFRGLQSRIELEQKFSIIRNGQLIQLPVAEIVVGDIAQVKYGDLLPADGILIQGNDLKIDESSLTGESDHVKKTLDKDPMLLSGTHVMEGSGRMVVTAVGINSQTGIIFTLLGANEEEDDEKKKKGKKQGVSENRNKAKTQDGVALEIQPLNSQEGLDSEEKEKKASKGPKKEKSVLQGKLTRLAVQIGKAGLIMSILTVLILILYFVVDNFVIQRRAWLPECTPVYIQYFVKFFIIGVTVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTMNRMTVVQAYIGGTHYRQIPKPDDLPPNVLDLIVNSICINSAYTSKILPPEKEGGLPRQVGNKTECGLLGFVTDLKQDYQAVRSEMPEEKLFKVYTFNSVRKSMSTVIRKPEGGFRVFSKGASEIMLRKCDRILNKEGGIVPFKTKDRDNMVRNVIEPMASEGLRTIGIAYRDFDGEEPSWENENEIFTGLVCIAVVGIEDPVRPEVPDAINKCKRAGITVRMVTGDNVNTARAIATKCGILTPGDDFLCLEGKEFNRLIRNEKGEVEQEKLDKVWPRLRVLARSSPTDKHTLVKGIIDSNIGEQRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFTSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVIVAFSGACITQDSPLKAVQMLWVNLIMDTFASLALATEPPTDSLLRRRPYGRNKPLISRTMMKNILGHAVYQLGIVFLLVFAGDKLFDIDSGRKAPLNSPPSQHYTIVFNTFVLMQLFNEINSRKIHGEKNVFAGVYRNIIFCSVVLGTFFCQILIVEVGGKPFSCTNLTMEQWMWCLFIGIGELLWGQVISAIPTKSLKFLKEAGHGSDKEEISKDAEGLEEIDHAEMELRRGQILWVRGLNRIQTQIEVINKFQTGASFKGVLRRQNLSQQLDVKLVPSSYSEAVASVRTSPSTSSAVTPPPVGNQSGQSIS

Target ORF information:

RefSeq Version	XM_008769449.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ATPase plasma membrane Ca2+ transporting 4 (Atp2b4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008769449.2

ORF Insert Sequence:

ATGACGAATC CATCAGGACA TAACTTGCCA GCCAACTCGG TAGCTGAGAG CTATGAAGGG 
GAGTTTGGCT GCACCTTAAT GGACCTGAGG AAGCTCATGG AGCTTCGTGC AGCTGATGCA 
GTGACCCAGA TCAGTGCCCA CTATGGAAGT GTACAAGAAA TCTGCGCAAG ACTGAAAACC 
TCCCCTGTAG AAGGTCTGTC TGGGAACCCT GCAGATTTGG AGAAGCGTAG ACTTGTTTTT 
GGGAAGAACA TGATACCTCC GAAAAAGCCC AAGACGTTCT TAGAATTAGT GTGGGAAGCC 
CTGCAGGATG TCACACTCAT CATCCTGGAG ATCGCAGCCA TCATATCCCT GGTCCTGTCC 
TTCTACCGGC CTCCTGGCGG GGAAAATGAA ATCTGTGGCC ACATTGTAAG TAACCCAGAA 
GAAGATGAGG AAGGAGAAAC TGGCTGGATT GAAGGGGCAG CCATTCTTGC CTCGGTGATC 
ATTGTGGTAT TCGTGACAGC CTTCAACGAC TGGAGCAAAG AGAAGCAGTT CCGGGGGCTG 
CAGAGTCGCA TTGAGCTTGA GCAAAAATTC TCCATTATCC GGAATGGTCA GCTTATACAA 
CTCCCAGTGG CTGAGATTGT GGTGGGAGAT ATTGCCCAGG TCAAATACGG TGACCTGCTG 
CCTGCCGACG GAATCCTAAT CCAGGGAAAT GATCTGAAGA TTGACGAGAG CTCTTTGACA 
GGAGAATCAG ATCATGTCAA GAAGACTCTG GACAAAGACC CCATGTTGCT CTCAGGGACT 
CATGTGATGG AAGGCTCTGG ACGGATGGTG GTGACTGCTG TGGGAATCAA TTCCCAGACT 
GGAATCATCT TCACCCTCTT AGGGGCTAAT GAGGAAGAAG ACGATGAGAA AAAGAAGAAA 
GGTAAAAAAC AAGGAGTCTC TGAAAATCGC AACAAAGCAA AAACCCAGGA TGGAGTGGCC 
TTGGAAATCC AGCCACTCAA CAGCCAGGAG GGGCTCGATA GTGAGGAAAA GGAGAAGAAA 
GCAAGCAAGG GACCCAAGAA GGAGAAGTCG GTCCTGCAGG GCAAGCTCAC ACGCCTGGCT 
GTGCAGATCG GGAAGGCAGG TCTGATCATG TCTATCCTCA CAGTCCTCAT CCTGATTCTA 
TACTTCGTGG TTGACAATTT CGTGATACAG CGCCGGGCAT GGCTTCCTGA GTGCACTCCT 
GTGTACATCC AGTATTTTGT CAAGTTCTTC ATCATCGGAG TCACTGTACT GGTAGTGGCT 
GTGCCAGAGG GGCTGCCACT GGCTGTCACC ATCTCGTTGG CCTACTCTGT AAAGAAAATG 
ATGAAGGACA ACAACTTGGT CCGGCACTTG GACGCCTGTG AGACGATGGG CAACGCCACA 
GCCATCTGCT CTGATAAGAC GGGCACGCTG ACCATGAACC GCATGACCGT GGTACAAGCT 
TACATTGGGG GCACTCACTA CCGCCAGATC CCAAAACCTG ACGACCTCCC ACCCAACGTT 
CTGGACCTCA TAGTCAACAG CATCTGTATC AACTCTGCTT ACACTTCTAA GATTCTGCCT 
CCAGAGAAAG AGGGAGGCCT CCCCCGGCAA GTAGGCAACA AGACAGAGTG TGGACTGCTG 
GGCTTTGTCA CAGACCTGAA GCAGGACTAC CAAGCAGTTC GCAGTGAGAT GCCCGAGGAG 
AAGCTCTTCA AGGTGTACAC CTTCAACTCA GTGCGCAAGT CCATGAGCAC CGTCATCAGG 
AAGCCGGAAG GGGGCTTCCG CGTGTTCAGC AAGGGCGCCT CCGAGATAAT GCTGCGCAAG 
TGTGATCGGA TCCTGAACAA GGAAGGAGGA ATTGTACCAT TCAAAACTAA GGACCGAGAC 
AATATGGTAC GCAACGTCAT TGAGCCCATG GCCAGTGAAG GACTCCGGAC CATCGGCATA 
GCTTACCGAG ATTTTGATGG TGAAGAGCCC TCGTGGGAAA ATGAGAATGA GATCTTCACT 
GGGCTGGTCT GCATTGCAGT GGTGGGCATT GAGGACCCTG TGCGCCCAGA GGTACCAGAT 
GCCATTAACA AGTGCAAACG GGCTGGCATT ACTGTCAGGA TGGTGACAGG TGACAACGTC 
AACACAGCCA GGGCCATCGC CACCAAATGT GGCATCTTGA CTCCTGGTGA TGACTTCCTG 
TGTCTGGAAG GGAAAGAATT CAACAGACTC ATACGCAATG AGAAGGGCGA GGTCGAACAA 
GAAAAGCTGG ACAAGGTCTG GCCCAGGCTT CGGGTACTGG CACGATCTTC TCCCACAGAC 
AAGCACACAT TGGTGAAAGG TATCATTGAC AGCAATATTG GGGAACAGAG GCAGGTGGTG 
GCTGTCACCG GTGATGGAAC CAATGATGGA CCGGCTCTAA AGAAAGCAGA TGTCGGTTTT 
GCTATGGGCA TTGCAGGCAC AGATGTGGCT AAGGAGGCAT CAGACATCAT CCTGACTGAT 
GACAACTTCA CCAGCATTGT GAAGGCAGTG ATGTGGGGCC GGAACGTATA CGACAGCATT 
TCCAAGTTCC TGCAGTTCCA GCTCACTGTC AACGTGGTGG CCGTGATTGT GGCTTTCTCT 
GGAGCCTGCA TCACTCAGGA TTCCCCACTG AAAGCGGTGC AGATGCTGTG GGTGAACCTG 
ATCATGGACA CGTTTGCTTC TCTGGCCCTG GCCACAGAGC CCCCGACCGA TTCCCTGCTG 
AGGCGTCGCC CCTATGGGCG AAACAAGCCC CTGATCTCTC GCACAATGAT GAAGAACATC 
CTGGGCCACG CGGTGTATCA GCTCGGAATC GTCTTCCTCC TCGTCTTTGC TGGCGACAAA 
CTGTTTGACA TTGACAGTGG GAGGAAGGCG CCTCTCAATT CACCACCCAG CCAGCACTAC 
ACCATTGTTT TCAACACGTT TGTGCTGATG CAACTCTTCA ACGAGATCAA CTCTCGGAAG 
ATCCATGGCG AGAAGAATGT CTTTGCAGGT GTCTACCGCA ACATCATTTT CTGCTCTGTT 
GTTTTAGGCA CGTTCTTCTG CCAGATCTTG ATCGTGGAAG TGGGAGGTAA GCCCTTCAGC 
TGCACAAACC TCACCATGGA GCAGTGGATG TGGTGTCTCT TTATAGGGAT CGGAGAACTT 
TTGTGGGGCC AGGTCATCTC TGCAATACCT ACCAAGTCTC TAAAGTTCCT GAAAGAGGCG 
GGCCATGGCA GTGACAAAGA GGAGATCAGC AAGGACGCTG AGGGGCTGGA AGAGATCGAC 
CATGCCGAGA TGGAGCTTCG CCGAGGCCAG ATCCTCTGGG TCCGTGGCCT GAACCGGATT 
CAGACTCAGA TTGAAGTAAT TAACAAATTC CAGACGGGAG CCTCTTTTAA GGGAGTCCTA 
AGGCGACAGA ACTTGAGTCA ACAACTTGAT GTAAAACTGG TTCCTAGCTC CTATTCAGAA 
GCAGTTGCGT CAGTCAGAAC CTCCCCCTCC ACCTCTTCTG CCGTTACACC TCCTCCTGTG 
GGCAATCAGA GTGGTCAAAG TATTTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Atp2b4 ( XM_008769447.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008767669.1 Rattus norvegicus ATPase plasma membrane Ca2+ transporting 4 (Atp2b4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa43325
Clone ID Related Accession (Same CDS sequence)	XM_008769447.2 , XM_008769446.2
Accession Version	XM_008769447.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3612bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	plasma membrane calcium-transporting ATPase 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008769447.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGAATC CATCAGGACA TAACTTGCCA GCCAACTCGG TAGCTGAGAG CTATGAAGGG 
GAGTTTGGCT GCACCTTAAT GGACCTGAGG AAGCTCATGG AGCTTCGTGC AGCTGATGCA 
GTGACCCAGA TCAGTGCCCA CTATGGAAGT GTACAAGAAA TCTGCGCAAG ACTGAAAACC 
TCCCCTGTAG AAGGTCTGTC TGGGAACCCT GCAGATTTGG AGAAGCGTAG ACTTGTTTTT 
GGGAAGAACA TGATACCTCC GAAAAAGCCC AAGACGTTCT TAGAATTAGT GTGGGAAGCC 
CTGCAGGATG TCACACTCAT CATCCTGGAG ATCGCAGCCA TCATATCCCT GGTCCTGTCC 
TTCTACCGGC CTCCTGGCGG GGAAAATGAA ATCTGTGGCC ACATTGTAAG TAACCCAGAA 
GAAGATGAGG AAGGAGAAAC TGGCTGGATT GAAGGGGCAG CCATTCTTGC CTCGGTGATC 
ATTGTGGTAT TCGTGACAGC CTTCAACGAC TGGAGCAAAG AGAAGCAGTT CCGGGGGCTG 
CAGAGTCGCA TTGAGCTTGA GCAAAAATTC TCCATTATCC GGAATGGTCA GCTTATACAA 
CTCCCAGTGG CTGAGATTGT GGTGGGAGAT ATTGCCCAGG TCAAATACGG TGACCTGCTG 
CCTGCCGACG GAATCCTAAT CCAGGGAAAT GATCTGAAGA TTGACGAGAG CTCTTTGACA 
GGAGAATCAG ATCATGTCAA GAAGACTCTG GACAAAGACC CCATGTTGCT CTCAGGGACT 
CATGTGATGG AAGGCTCTGG ACGGATGGTG GTGACTGCTG TGGGAATCAA TTCCCAGACT 
GGAATCATCT TCACCCTCTT AGGGGCTAAT GAGGAAGAAG ACGATGAGAA AAAGAAGAAA 
GGTAAAAAAC AAGGAGTCTC TGAAAATCGC AACAAAGCAA AAACCCAGGA TGGAGTGGCC 
TTGGAAATCC AGCCACTCAA CAGCCAGGAG GGGCTCGATA GTGAGGAAAA GGAGAAGAAA 
GCAAGCAAGG GACCCAAGAA GGAGAAGTCG GTCCTGCAGG GCAAGCTCAC ACGCCTGGCT 
GTGCAGATCG GGAAGGCAGG TCTGATCATG TCTATCCTCA CAGTCCTCAT CCTGATTCTA 
TACTTCGTGG TTGACAATTT CGTGATACAG CGCCGGGCAT GGCTTCCTGA GTGCACTCCT 
GTGTACATCC AGTATTTTGT CAAGTTCTTC ATCATCGGAG TCACTGTACT GGTAGTGGCT 
GTGCCAGAGG GGCTGCCACT GGCTGTCACC ATCTCGTTGG CCTACTCTGT AAAGAAAATG 
ATGAAGGACA ACAACTTGGT CCGGCACTTG GACGCCTGTG AGACGATGGG CAACGCCACA 
GCCATCTGCT CTGATAAGAC GGGCACGCTG ACCATGAACC GCATGACCGT GGTACAAGCT 
TACATTGGGG GCACTCACTA CCGCCAGATC CCAAAACCTG ACGACCTCCC ACCCAACGTT 
CTGGACCTCA TAGTCAACAG CATCTGTATC AACTCTGCTT ACACTTCTAA GATTCTGCCT 
CCAGAGAAAG AGGGAGGCCT CCCCCGGCAA GTAGGCAACA AGACAGAGTG TGGACTGCTG 
GGCTTTGTCA CAGACCTGAA GCAGGACTAC CAAGCAGTTC GCAGTGAGAT GCCCGAGGAG 
AAGCTCTTCA AGGTGTACAC CTTCAACTCA GTGCGCAAGT CCATGAGCAC CGTCATCAGG 
AAGCCGGAAG GGGGCTTCCG CGTGTTCAGC AAGGGCGCCT CCGAGATAAT GCTGCGCAAG 
TGTGATCGGA TCCTGAACAA GGAAGGAGGA ATTGTACCAT TCAAAACTAA GGACCGAGAC 
AATATGGTAC GCAACGTCAT TGAGCCCATG GCCAGTGAAG GACTCCGGAC CATCGGCATA 
GCTTACCGAG ATTTTGATGG TGAAGAGCCC TCGTGGGAAA ATGAGAATGA GATCTTCACT 
GGGCTGGTCT GCATTGCAGT GGTGGGCATT GAGGACCCTG TGCGCCCAGA GGTACCAGAT 
GCCATTAACA AGTGCAAACG GGCTGGCATT ACTGTCAGGA TGGTGACAGG TGACAACGTC 
AACACAGCCA GGGCCATCGC CACCAAATGT GGCATCTTGA CTCCTGGTGA TGACTTCCTG 
TGTCTGGAAG GGAAAGAATT CAACAGACTC ATACGCAATG AGAAGGGCGA GGTCGAACAA 
GAAAAGCTGG ACAAGGTCTG GCCCAGGCTT CGGGTACTGG CACGATCTTC TCCCACAGAC 
AAGCACACAT TGGTGAAAGG TATCATTGAC AGCAATATTG GGGAACAGAG GCAGGTGGTG 
GCTGTCACCG GTGATGGAAC CAATGATGGA CCGGCTCTAA AGAAAGCAGA TGTCGGTTTT 
GCTATGGGCA TTGCAGGCAC AGATGTGGCT AAGGAGGCAT CAGACATCAT CCTGACTGAT 
GACAACTTCA CCAGCATTGT GAAGGCAGTG ATGTGGGGCC GGAACGTATA CGACAGCATT 
TCCAAGTTCC TGCAGTTCCA GCTCACTGTC AACGTGGTGG CCGTGATTGT GGCTTTCTCT 
GGAGCCTGCA TCACTCAGGA TTCCCCACTG AAAGCGGTGC AGATGCTGTG GGTGAACCTG 
ATCATGGACA CGTTTGCTTC TCTGGCCCTG GCCACAGAGC CCCCGACCGA TTCCCTGCTG 
AGGCGTCGCC CCTATGGGCG AAACAAGCCC CTGATCTCTC GCACAATGAT GAAGAACATC 
CTGGGCCACG CGGTGTATCA GCTCGGAATC GTCTTCCTCC TCGTCTTTGC TGGCGACAAA 
CTGTTTGACA TTGACAGTGG GAGGAAGGCG CCTCTCAATT CACCACCCAG CCAGCACTAC 
ACCATTGTTT TCAACACGTT TGTGCTGATG CAACTCTTCA ACGAGATCAA CTCTCGGAAG 
ATCCATGGCG AGAAGAATGT CTTTGCAGGT GTCTACCGCA ACATCATTTT CTGCTCTGTT 
GTTTTAGGCA CGTTCTTCTG CCAGATCTTG ATCGTGGAAG TGGGAGGTAA GCCCTTCAGC 
TGCACAAACC TCACCATGGA GCAGTGGATG TGGTGTCTCT TTATAGGGAT CGGAGAACTT 
TTGTGGGGCC AGGTCATCTC TGCAATACCT ACCAAGTCTC TAAAGTTCCT GAAAGAGGCG 
GGCCATGGCA GTGACAAAGA GGAGATCAGC AAGGACGCTG AGGGGCTGGA AGAGATCGAC 
CATGCCGAGA TGGAGCTTCG CCGAGGCCAG ATCCTCTGGG TCCGTGGCCT GAACCGGATT 
CAGACTCAGA TCAGAGTGGT CAAAGTATTT CATAGTTTCC GAGACGTCAT TCATAAATCC 
AAGAACCAGG TCTCCATCCA CAGCTTCATG ACCCAACCTG AGTACGCCGC AGACGATGAA 
ATGTCACAGT CCTTCCTGAA TCAAGAGGAA AGTCCTTCGT TGGCTTCTAA GTCGCGGATT 
ACCAAGCGCC TGTCGGATGC TGAAACTGTT TCACAGAACA ACACGAATAA CAATGCCGTG 
GACTGCCACC AAGTGCAAAT CGTTGCCTCC CATCCCAACA GCCCTCTACA AAGCCAGGAG 
ACACCGGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008767669.1
CDS	1192..4803
Translation	MTNPSGHNLPANSVAESYEGEFGCTLMDLRKLMELRAADAVTQISAHYGSVQEICARLKTSPVEGLSGNPADLEKRRLVFGKNMIPPKKPKTFLELVWEALQDVTLIILEIAAIISLVLSFYRPPGGENEICGHIVSNPEEDEEGETGWIEGAAILASVIIVVFVTAFNDWSKEKQFRGLQSRIELEQKFSIIRNGQLIQLPVAEIVVGDIAQVKYGDLLPADGILIQGNDLKIDESSLTGESDHVKKTLDKDPMLLSGTHVMEGSGRMVVTAVGINSQTGIIFTLLGANEEEDDEKKKKGKKQGVSENRNKAKTQDGVALEIQPLNSQEGLDSEEKEKKASKGPKKEKSVLQGKLTRLAVQIGKAGLIMSILTVLILILYFVVDNFVIQRRAWLPECTPVYIQYFVKFFIIGVTVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTMNRMTVVQAYIGGTHYRQIPKPDDLPPNVLDLIVNSICINSAYTSKILPPEKEGGLPRQVGNKTECGLLGFVTDLKQDYQAVRSEMPEEKLFKVYTFNSVRKSMSTVIRKPEGGFRVFSKGASEIMLRKCDRILNKEGGIVPFKTKDRDNMVRNVIEPMASEGLRTIGIAYRDFDGEEPSWENENEIFTGLVCIAVVGIEDPVRPEVPDAINKCKRAGITVRMVTGDNVNTARAIATKCGILTPGDDFLCLEGKEFNRLIRNEKGEVEQEKLDKVWPRLRVLARSSPTDKHTLVKGIIDSNIGEQRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFTSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVIVAFSGACITQDSPLKAVQMLWVNLIMDTFASLALATEPPTDSLLRRRPYGRNKPLISRTMMKNILGHAVYQLGIVFLLVFAGDKLFDIDSGRKAPLNSPPSQHYTIVFNTFVLMQLFNEINSRKIHGEKNVFAGVYRNIIFCSVVLGTFFCQILIVEVGGKPFSCTNLTMEQWMWCLFIGIGELLWGQVISAIPTKSLKFLKEAGHGSDKEEISKDAEGLEEIDHAEMELRRGQILWVRGLNRIQTQIRVVKVFHSFRDVIHKSKNQVSIHSFMTQPEYAADDEMSQSFLNQEESPSLASKSRITKRLSDAETVSQNNTNNNAVDCHQVQIVASHPNSPLQSQETPV

Target ORF information:

RefSeq Version	XM_008769447.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ATPase plasma membrane Ca2+ transporting 4 (Atp2b4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008769447.2

ORF Insert Sequence:

ATGACGAATC CATCAGGACA TAACTTGCCA GCCAACTCGG TAGCTGAGAG CTATGAAGGG 
GAGTTTGGCT GCACCTTAAT GGACCTGAGG AAGCTCATGG AGCTTCGTGC AGCTGATGCA 
GTGACCCAGA TCAGTGCCCA CTATGGAAGT GTACAAGAAA TCTGCGCAAG ACTGAAAACC 
TCCCCTGTAG AAGGTCTGTC TGGGAACCCT GCAGATTTGG AGAAGCGTAG ACTTGTTTTT 
GGGAAGAACA TGATACCTCC GAAAAAGCCC AAGACGTTCT TAGAATTAGT GTGGGAAGCC 
CTGCAGGATG TCACACTCAT CATCCTGGAG ATCGCAGCCA TCATATCCCT GGTCCTGTCC 
TTCTACCGGC CTCCTGGCGG GGAAAATGAA ATCTGTGGCC ACATTGTAAG TAACCCAGAA 
GAAGATGAGG AAGGAGAAAC TGGCTGGATT GAAGGGGCAG CCATTCTTGC CTCGGTGATC 
ATTGTGGTAT TCGTGACAGC CTTCAACGAC TGGAGCAAAG AGAAGCAGTT CCGGGGGCTG 
CAGAGTCGCA TTGAGCTTGA GCAAAAATTC TCCATTATCC GGAATGGTCA GCTTATACAA 
CTCCCAGTGG CTGAGATTGT GGTGGGAGAT ATTGCCCAGG TCAAATACGG TGACCTGCTG 
CCTGCCGACG GAATCCTAAT CCAGGGAAAT GATCTGAAGA TTGACGAGAG CTCTTTGACA 
GGAGAATCAG ATCATGTCAA GAAGACTCTG GACAAAGACC CCATGTTGCT CTCAGGGACT 
CATGTGATGG AAGGCTCTGG ACGGATGGTG GTGACTGCTG TGGGAATCAA TTCCCAGACT 
GGAATCATCT TCACCCTCTT AGGGGCTAAT GAGGAAGAAG ACGATGAGAA AAAGAAGAAA 
GGTAAAAAAC AAGGAGTCTC TGAAAATCGC AACAAAGCAA AAACCCAGGA TGGAGTGGCC 
TTGGAAATCC AGCCACTCAA CAGCCAGGAG GGGCTCGATA GTGAGGAAAA GGAGAAGAAA 
GCAAGCAAGG GACCCAAGAA GGAGAAGTCG GTCCTGCAGG GCAAGCTCAC ACGCCTGGCT 
GTGCAGATCG GGAAGGCAGG TCTGATCATG TCTATCCTCA CAGTCCTCAT CCTGATTCTA 
TACTTCGTGG TTGACAATTT CGTGATACAG CGCCGGGCAT GGCTTCCTGA GTGCACTCCT 
GTGTACATCC AGTATTTTGT CAAGTTCTTC ATCATCGGAG TCACTGTACT GGTAGTGGCT 
GTGCCAGAGG GGCTGCCACT GGCTGTCACC ATCTCGTTGG CCTACTCTGT AAAGAAAATG 
ATGAAGGACA ACAACTTGGT CCGGCACTTG GACGCCTGTG AGACGATGGG CAACGCCACA 
GCCATCTGCT CTGATAAGAC GGGCACGCTG ACCATGAACC GCATGACCGT GGTACAAGCT 
TACATTGGGG GCACTCACTA CCGCCAGATC CCAAAACCTG ACGACCTCCC ACCCAACGTT 
CTGGACCTCA TAGTCAACAG CATCTGTATC AACTCTGCTT ACACTTCTAA GATTCTGCCT 
CCAGAGAAAG AGGGAGGCCT CCCCCGGCAA GTAGGCAACA AGACAGAGTG TGGACTGCTG 
GGCTTTGTCA CAGACCTGAA GCAGGACTAC CAAGCAGTTC GCAGTGAGAT GCCCGAGGAG 
AAGCTCTTCA AGGTGTACAC CTTCAACTCA GTGCGCAAGT CCATGAGCAC CGTCATCAGG 
AAGCCGGAAG GGGGCTTCCG CGTGTTCAGC AAGGGCGCCT CCGAGATAAT GCTGCGCAAG 
TGTGATCGGA TCCTGAACAA GGAAGGAGGA ATTGTACCAT TCAAAACTAA GGACCGAGAC 
AATATGGTAC GCAACGTCAT TGAGCCCATG GCCAGTGAAG GACTCCGGAC CATCGGCATA 
GCTTACCGAG ATTTTGATGG TGAAGAGCCC TCGTGGGAAA ATGAGAATGA GATCTTCACT 
GGGCTGGTCT GCATTGCAGT GGTGGGCATT GAGGACCCTG TGCGCCCAGA GGTACCAGAT 
GCCATTAACA AGTGCAAACG GGCTGGCATT ACTGTCAGGA TGGTGACAGG TGACAACGTC 
AACACAGCCA GGGCCATCGC CACCAAATGT GGCATCTTGA CTCCTGGTGA TGACTTCCTG 
TGTCTGGAAG GGAAAGAATT CAACAGACTC ATACGCAATG AGAAGGGCGA GGTCGAACAA 
GAAAAGCTGG ACAAGGTCTG GCCCAGGCTT CGGGTACTGG CACGATCTTC TCCCACAGAC 
AAGCACACAT TGGTGAAAGG TATCATTGAC AGCAATATTG GGGAACAGAG GCAGGTGGTG 
GCTGTCACCG GTGATGGAAC CAATGATGGA CCGGCTCTAA AGAAAGCAGA TGTCGGTTTT 
GCTATGGGCA TTGCAGGCAC AGATGTGGCT AAGGAGGCAT CAGACATCAT CCTGACTGAT 
GACAACTTCA CCAGCATTGT GAAGGCAGTG ATGTGGGGCC GGAACGTATA CGACAGCATT 
TCCAAGTTCC TGCAGTTCCA GCTCACTGTC AACGTGGTGG CCGTGATTGT GGCTTTCTCT 
GGAGCCTGCA TCACTCAGGA TTCCCCACTG AAAGCGGTGC AGATGCTGTG GGTGAACCTG 
ATCATGGACA CGTTTGCTTC TCTGGCCCTG GCCACAGAGC CCCCGACCGA TTCCCTGCTG 
AGGCGTCGCC CCTATGGGCG AAACAAGCCC CTGATCTCTC GCACAATGAT GAAGAACATC 
CTGGGCCACG CGGTGTATCA GCTCGGAATC GTCTTCCTCC TCGTCTTTGC TGGCGACAAA 
CTGTTTGACA TTGACAGTGG GAGGAAGGCG CCTCTCAATT CACCACCCAG CCAGCACTAC 
ACCATTGTTT TCAACACGTT TGTGCTGATG CAACTCTTCA ACGAGATCAA CTCTCGGAAG 
ATCCATGGCG AGAAGAATGT CTTTGCAGGT GTCTACCGCA ACATCATTTT CTGCTCTGTT 
GTTTTAGGCA CGTTCTTCTG CCAGATCTTG ATCGTGGAAG TGGGAGGTAA GCCCTTCAGC 
TGCACAAACC TCACCATGGA GCAGTGGATG TGGTGTCTCT TTATAGGGAT CGGAGAACTT 
TTGTGGGGCC AGGTCATCTC TGCAATACCT ACCAAGTCTC TAAAGTTCCT GAAAGAGGCG 
GGCCATGGCA GTGACAAAGA GGAGATCAGC AAGGACGCTG AGGGGCTGGA AGAGATCGAC 
CATGCCGAGA TGGAGCTTCG CCGAGGCCAG ATCCTCTGGG TCCGTGGCCT GAACCGGATT 
CAGACTCAGA TCAGAGTGGT CAAAGTATTT CATAGTTTCC GAGACGTCAT TCATAAATCC 
AAGAACCAGG TCTCCATCCA CAGCTTCATG ACCCAACCTG AGTACGCCGC AGACGATGAA 
ATGTCACAGT CCTTCCTGAA TCAAGAGGAA AGTCCTTCGT TGGCTTCTAA GTCGCGGATT 
ACCAAGCGCC TGTCGGATGC TGAAACTGTT TCACAGAACA ACACGAATAA CAATGCCGTG 
GACTGCCACC AAGTGCAAAT CGTTGCCTCC CATCCCAACA GCCCTCTACA AAGCCAGGAG 
ACACCGGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Atp2b4 ( XM_008769446.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008767668.1 Rattus norvegicus ATPase plasma membrane Ca2+ transporting 4 (Atp2b4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa43325
Clone ID Related Accession (Same CDS sequence)	XM_008769447.2 , XM_008769446.2
Accession Version	XM_008769446.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3612bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	plasma membrane calcium-transporting ATPase 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008769446.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGAATC CATCAGGACA TAACTTGCCA GCCAACTCGG TAGCTGAGAG CTATGAAGGG 
GAGTTTGGCT GCACCTTAAT GGACCTGAGG AAGCTCATGG AGCTTCGTGC AGCTGATGCA 
GTGACCCAGA TCAGTGCCCA CTATGGAAGT GTACAAGAAA TCTGCGCAAG ACTGAAAACC 
TCCCCTGTAG AAGGTCTGTC TGGGAACCCT GCAGATTTGG AGAAGCGTAG ACTTGTTTTT 
GGGAAGAACA TGATACCTCC GAAAAAGCCC AAGACGTTCT TAGAATTAGT GTGGGAAGCC 
CTGCAGGATG TCACACTCAT CATCCTGGAG ATCGCAGCCA TCATATCCCT GGTCCTGTCC 
TTCTACCGGC CTCCTGGCGG GGAAAATGAA ATCTGTGGCC ACATTGTAAG TAACCCAGAA 
GAAGATGAGG AAGGAGAAAC TGGCTGGATT GAAGGGGCAG CCATTCTTGC CTCGGTGATC 
ATTGTGGTAT TCGTGACAGC CTTCAACGAC TGGAGCAAAG AGAAGCAGTT CCGGGGGCTG 
CAGAGTCGCA TTGAGCTTGA GCAAAAATTC TCCATTATCC GGAATGGTCA GCTTATACAA 
CTCCCAGTGG CTGAGATTGT GGTGGGAGAT ATTGCCCAGG TCAAATACGG TGACCTGCTG 
CCTGCCGACG GAATCCTAAT CCAGGGAAAT GATCTGAAGA TTGACGAGAG CTCTTTGACA 
GGAGAATCAG ATCATGTCAA GAAGACTCTG GACAAAGACC CCATGTTGCT CTCAGGGACT 
CATGTGATGG AAGGCTCTGG ACGGATGGTG GTGACTGCTG TGGGAATCAA TTCCCAGACT 
GGAATCATCT TCACCCTCTT AGGGGCTAAT GAGGAAGAAG ACGATGAGAA AAAGAAGAAA 
GGTAAAAAAC AAGGAGTCTC TGAAAATCGC AACAAAGCAA AAACCCAGGA TGGAGTGGCC 
TTGGAAATCC AGCCACTCAA CAGCCAGGAG GGGCTCGATA GTGAGGAAAA GGAGAAGAAA 
GCAAGCAAGG GACCCAAGAA GGAGAAGTCG GTCCTGCAGG GCAAGCTCAC ACGCCTGGCT 
GTGCAGATCG GGAAGGCAGG TCTGATCATG TCTATCCTCA CAGTCCTCAT CCTGATTCTA 
TACTTCGTGG TTGACAATTT CGTGATACAG CGCCGGGCAT GGCTTCCTGA GTGCACTCCT 
GTGTACATCC AGTATTTTGT CAAGTTCTTC ATCATCGGAG TCACTGTACT GGTAGTGGCT 
GTGCCAGAGG GGCTGCCACT GGCTGTCACC ATCTCGTTGG CCTACTCTGT AAAGAAAATG 
ATGAAGGACA ACAACTTGGT CCGGCACTTG GACGCCTGTG AGACGATGGG CAACGCCACA 
GCCATCTGCT CTGATAAGAC GGGCACGCTG ACCATGAACC GCATGACCGT GGTACAAGCT 
TACATTGGGG GCACTCACTA CCGCCAGATC CCAAAACCTG ACGACCTCCC ACCCAACGTT 
CTGGACCTCA TAGTCAACAG CATCTGTATC AACTCTGCTT ACACTTCTAA GATTCTGCCT 
CCAGAGAAAG AGGGAGGCCT CCCCCGGCAA GTAGGCAACA AGACAGAGTG TGGACTGCTG 
GGCTTTGTCA CAGACCTGAA GCAGGACTAC CAAGCAGTTC GCAGTGAGAT GCCCGAGGAG 
AAGCTCTTCA AGGTGTACAC CTTCAACTCA GTGCGCAAGT CCATGAGCAC CGTCATCAGG 
AAGCCGGAAG GGGGCTTCCG CGTGTTCAGC AAGGGCGCCT CCGAGATAAT GCTGCGCAAG 
TGTGATCGGA TCCTGAACAA GGAAGGAGGA ATTGTACCAT TCAAAACTAA GGACCGAGAC 
AATATGGTAC GCAACGTCAT TGAGCCCATG GCCAGTGAAG GACTCCGGAC CATCGGCATA 
GCTTACCGAG ATTTTGATGG TGAAGAGCCC TCGTGGGAAA ATGAGAATGA GATCTTCACT 
GGGCTGGTCT GCATTGCAGT GGTGGGCATT GAGGACCCTG TGCGCCCAGA GGTACCAGAT 
GCCATTAACA AGTGCAAACG GGCTGGCATT ACTGTCAGGA TGGTGACAGG TGACAACGTC 
AACACAGCCA GGGCCATCGC CACCAAATGT GGCATCTTGA CTCCTGGTGA TGACTTCCTG 
TGTCTGGAAG GGAAAGAATT CAACAGACTC ATACGCAATG AGAAGGGCGA GGTCGAACAA 
GAAAAGCTGG ACAAGGTCTG GCCCAGGCTT CGGGTACTGG CACGATCTTC TCCCACAGAC 
AAGCACACAT TGGTGAAAGG TATCATTGAC AGCAATATTG GGGAACAGAG GCAGGTGGTG 
GCTGTCACCG GTGATGGAAC CAATGATGGA CCGGCTCTAA AGAAAGCAGA TGTCGGTTTT 
GCTATGGGCA TTGCAGGCAC AGATGTGGCT AAGGAGGCAT CAGACATCAT CCTGACTGAT 
GACAACTTCA CCAGCATTGT GAAGGCAGTG ATGTGGGGCC GGAACGTATA CGACAGCATT 
TCCAAGTTCC TGCAGTTCCA GCTCACTGTC AACGTGGTGG CCGTGATTGT GGCTTTCTCT 
GGAGCCTGCA TCACTCAGGA TTCCCCACTG AAAGCGGTGC AGATGCTGTG GGTGAACCTG 
ATCATGGACA CGTTTGCTTC TCTGGCCCTG GCCACAGAGC CCCCGACCGA TTCCCTGCTG 
AGGCGTCGCC CCTATGGGCG AAACAAGCCC CTGATCTCTC GCACAATGAT GAAGAACATC 
CTGGGCCACG CGGTGTATCA GCTCGGAATC GTCTTCCTCC TCGTCTTTGC TGGCGACAAA 
CTGTTTGACA TTGACAGTGG GAGGAAGGCG CCTCTCAATT CACCACCCAG CCAGCACTAC 
ACCATTGTTT TCAACACGTT TGTGCTGATG CAACTCTTCA ACGAGATCAA CTCTCGGAAG 
ATCCATGGCG AGAAGAATGT CTTTGCAGGT GTCTACCGCA ACATCATTTT CTGCTCTGTT 
GTTTTAGGCA CGTTCTTCTG CCAGATCTTG ATCGTGGAAG TGGGAGGTAA GCCCTTCAGC 
TGCACAAACC TCACCATGGA GCAGTGGATG TGGTGTCTCT TTATAGGGAT CGGAGAACTT 
TTGTGGGGCC AGGTCATCTC TGCAATACCT ACCAAGTCTC TAAAGTTCCT GAAAGAGGCG 
GGCCATGGCA GTGACAAAGA GGAGATCAGC AAGGACGCTG AGGGGCTGGA AGAGATCGAC 
CATGCCGAGA TGGAGCTTCG CCGAGGCCAG ATCCTCTGGG TCCGTGGCCT GAACCGGATT 
CAGACTCAGA TCAGAGTGGT CAAAGTATTT CATAGTTTCC GAGACGTCAT TCATAAATCC 
AAGAACCAGG TCTCCATCCA CAGCTTCATG ACCCAACCTG AGTACGCCGC AGACGATGAA 
ATGTCACAGT CCTTCCTGAA TCAAGAGGAA AGTCCTTCGT TGGCTTCTAA GTCGCGGATT 
ACCAAGCGCC TGTCGGATGC TGAAACTGTT TCACAGAACA ACACGAATAA CAATGCCGTG 
GACTGCCACC AAGTGCAAAT CGTTGCCTCC CATCCCAACA GCCCTCTACA AAGCCAGGAG 
ACACCGGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008767668.1
CDS	546..4157
Translation	MTNPSGHNLPANSVAESYEGEFGCTLMDLRKLMELRAADAVTQISAHYGSVQEICARLKTSPVEGLSGNPADLEKRRLVFGKNMIPPKKPKTFLELVWEALQDVTLIILEIAAIISLVLSFYRPPGGENEICGHIVSNPEEDEEGETGWIEGAAILASVIIVVFVTAFNDWSKEKQFRGLQSRIELEQKFSIIRNGQLIQLPVAEIVVGDIAQVKYGDLLPADGILIQGNDLKIDESSLTGESDHVKKTLDKDPMLLSGTHVMEGSGRMVVTAVGINSQTGIIFTLLGANEEEDDEKKKKGKKQGVSENRNKAKTQDGVALEIQPLNSQEGLDSEEKEKKASKGPKKEKSVLQGKLTRLAVQIGKAGLIMSILTVLILILYFVVDNFVIQRRAWLPECTPVYIQYFVKFFIIGVTVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTMNRMTVVQAYIGGTHYRQIPKPDDLPPNVLDLIVNSICINSAYTSKILPPEKEGGLPRQVGNKTECGLLGFVTDLKQDYQAVRSEMPEEKLFKVYTFNSVRKSMSTVIRKPEGGFRVFSKGASEIMLRKCDRILNKEGGIVPFKTKDRDNMVRNVIEPMASEGLRTIGIAYRDFDGEEPSWENENEIFTGLVCIAVVGIEDPVRPEVPDAINKCKRAGITVRMVTGDNVNTARAIATKCGILTPGDDFLCLEGKEFNRLIRNEKGEVEQEKLDKVWPRLRVLARSSPTDKHTLVKGIIDSNIGEQRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFTSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVIVAFSGACITQDSPLKAVQMLWVNLIMDTFASLALATEPPTDSLLRRRPYGRNKPLISRTMMKNILGHAVYQLGIVFLLVFAGDKLFDIDSGRKAPLNSPPSQHYTIVFNTFVLMQLFNEINSRKIHGEKNVFAGVYRNIIFCSVVLGTFFCQILIVEVGGKPFSCTNLTMEQWMWCLFIGIGELLWGQVISAIPTKSLKFLKEAGHGSDKEEISKDAEGLEEIDHAEMELRRGQILWVRGLNRIQTQIRVVKVFHSFRDVIHKSKNQVSIHSFMTQPEYAADDEMSQSFLNQEESPSLASKSRITKRLSDAETVSQNNTNNNAVDCHQVQIVASHPNSPLQSQETPV

Target ORF information:

RefSeq Version	XM_008769446.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ATPase plasma membrane Ca2+ transporting 4 (Atp2b4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008769446.2

ORF Insert Sequence:

ATGACGAATC CATCAGGACA TAACTTGCCA GCCAACTCGG TAGCTGAGAG CTATGAAGGG 
GAGTTTGGCT GCACCTTAAT GGACCTGAGG AAGCTCATGG AGCTTCGTGC AGCTGATGCA 
GTGACCCAGA TCAGTGCCCA CTATGGAAGT GTACAAGAAA TCTGCGCAAG ACTGAAAACC 
TCCCCTGTAG AAGGTCTGTC TGGGAACCCT GCAGATTTGG AGAAGCGTAG ACTTGTTTTT 
GGGAAGAACA TGATACCTCC GAAAAAGCCC AAGACGTTCT TAGAATTAGT GTGGGAAGCC 
CTGCAGGATG TCACACTCAT CATCCTGGAG ATCGCAGCCA TCATATCCCT GGTCCTGTCC 
TTCTACCGGC CTCCTGGCGG GGAAAATGAA ATCTGTGGCC ACATTGTAAG TAACCCAGAA 
GAAGATGAGG AAGGAGAAAC TGGCTGGATT GAAGGGGCAG CCATTCTTGC CTCGGTGATC 
ATTGTGGTAT TCGTGACAGC CTTCAACGAC TGGAGCAAAG AGAAGCAGTT CCGGGGGCTG 
CAGAGTCGCA TTGAGCTTGA GCAAAAATTC TCCATTATCC GGAATGGTCA GCTTATACAA 
CTCCCAGTGG CTGAGATTGT GGTGGGAGAT ATTGCCCAGG TCAAATACGG TGACCTGCTG 
CCTGCCGACG GAATCCTAAT CCAGGGAAAT GATCTGAAGA TTGACGAGAG CTCTTTGACA 
GGAGAATCAG ATCATGTCAA GAAGACTCTG GACAAAGACC CCATGTTGCT CTCAGGGACT 
CATGTGATGG AAGGCTCTGG ACGGATGGTG GTGACTGCTG TGGGAATCAA TTCCCAGACT 
GGAATCATCT TCACCCTCTT AGGGGCTAAT GAGGAAGAAG ACGATGAGAA AAAGAAGAAA 
GGTAAAAAAC AAGGAGTCTC TGAAAATCGC AACAAAGCAA AAACCCAGGA TGGAGTGGCC 
TTGGAAATCC AGCCACTCAA CAGCCAGGAG GGGCTCGATA GTGAGGAAAA GGAGAAGAAA 
GCAAGCAAGG GACCCAAGAA GGAGAAGTCG GTCCTGCAGG GCAAGCTCAC ACGCCTGGCT 
GTGCAGATCG GGAAGGCAGG TCTGATCATG TCTATCCTCA CAGTCCTCAT CCTGATTCTA 
TACTTCGTGG TTGACAATTT CGTGATACAG CGCCGGGCAT GGCTTCCTGA GTGCACTCCT 
GTGTACATCC AGTATTTTGT CAAGTTCTTC ATCATCGGAG TCACTGTACT GGTAGTGGCT 
GTGCCAGAGG GGCTGCCACT GGCTGTCACC ATCTCGTTGG CCTACTCTGT AAAGAAAATG 
ATGAAGGACA ACAACTTGGT CCGGCACTTG GACGCCTGTG AGACGATGGG CAACGCCACA 
GCCATCTGCT CTGATAAGAC GGGCACGCTG ACCATGAACC GCATGACCGT GGTACAAGCT 
TACATTGGGG GCACTCACTA CCGCCAGATC CCAAAACCTG ACGACCTCCC ACCCAACGTT 
CTGGACCTCA TAGTCAACAG CATCTGTATC AACTCTGCTT ACACTTCTAA GATTCTGCCT 
CCAGAGAAAG AGGGAGGCCT CCCCCGGCAA GTAGGCAACA AGACAGAGTG TGGACTGCTG 
GGCTTTGTCA CAGACCTGAA GCAGGACTAC CAAGCAGTTC GCAGTGAGAT GCCCGAGGAG 
AAGCTCTTCA AGGTGTACAC CTTCAACTCA GTGCGCAAGT CCATGAGCAC CGTCATCAGG 
AAGCCGGAAG GGGGCTTCCG CGTGTTCAGC AAGGGCGCCT CCGAGATAAT GCTGCGCAAG 
TGTGATCGGA TCCTGAACAA GGAAGGAGGA ATTGTACCAT TCAAAACTAA GGACCGAGAC 
AATATGGTAC GCAACGTCAT TGAGCCCATG GCCAGTGAAG GACTCCGGAC CATCGGCATA 
GCTTACCGAG ATTTTGATGG TGAAGAGCCC TCGTGGGAAA ATGAGAATGA GATCTTCACT 
GGGCTGGTCT GCATTGCAGT GGTGGGCATT GAGGACCCTG TGCGCCCAGA GGTACCAGAT 
GCCATTAACA AGTGCAAACG GGCTGGCATT ACTGTCAGGA TGGTGACAGG TGACAACGTC 
AACACAGCCA GGGCCATCGC CACCAAATGT GGCATCTTGA CTCCTGGTGA TGACTTCCTG 
TGTCTGGAAG GGAAAGAATT CAACAGACTC ATACGCAATG AGAAGGGCGA GGTCGAACAA 
GAAAAGCTGG ACAAGGTCTG GCCCAGGCTT CGGGTACTGG CACGATCTTC TCCCACAGAC 
AAGCACACAT TGGTGAAAGG TATCATTGAC AGCAATATTG GGGAACAGAG GCAGGTGGTG 
GCTGTCACCG GTGATGGAAC CAATGATGGA CCGGCTCTAA AGAAAGCAGA TGTCGGTTTT 
GCTATGGGCA TTGCAGGCAC AGATGTGGCT AAGGAGGCAT CAGACATCAT CCTGACTGAT 
GACAACTTCA CCAGCATTGT GAAGGCAGTG ATGTGGGGCC GGAACGTATA CGACAGCATT 
TCCAAGTTCC TGCAGTTCCA GCTCACTGTC AACGTGGTGG CCGTGATTGT GGCTTTCTCT 
GGAGCCTGCA TCACTCAGGA TTCCCCACTG AAAGCGGTGC AGATGCTGTG GGTGAACCTG 
ATCATGGACA CGTTTGCTTC TCTGGCCCTG GCCACAGAGC CCCCGACCGA TTCCCTGCTG 
AGGCGTCGCC CCTATGGGCG AAACAAGCCC CTGATCTCTC GCACAATGAT GAAGAACATC 
CTGGGCCACG CGGTGTATCA GCTCGGAATC GTCTTCCTCC TCGTCTTTGC TGGCGACAAA 
CTGTTTGACA TTGACAGTGG GAGGAAGGCG CCTCTCAATT CACCACCCAG CCAGCACTAC 
ACCATTGTTT TCAACACGTT TGTGCTGATG CAACTCTTCA ACGAGATCAA CTCTCGGAAG 
ATCCATGGCG AGAAGAATGT CTTTGCAGGT GTCTACCGCA ACATCATTTT CTGCTCTGTT 
GTTTTAGGCA CGTTCTTCTG CCAGATCTTG ATCGTGGAAG TGGGAGGTAA GCCCTTCAGC 
TGCACAAACC TCACCATGGA GCAGTGGATG TGGTGTCTCT TTATAGGGAT CGGAGAACTT 
TTGTGGGGCC AGGTCATCTC TGCAATACCT ACCAAGTCTC TAAAGTTCCT GAAAGAGGCG 
GGCCATGGCA GTGACAAAGA GGAGATCAGC AAGGACGCTG AGGGGCTGGA AGAGATCGAC 
CATGCCGAGA TGGAGCTTCG CCGAGGCCAG ATCCTCTGGG TCCGTGGCCT GAACCGGATT 
CAGACTCAGA TCAGAGTGGT CAAAGTATTT CATAGTTTCC GAGACGTCAT TCATAAATCC 
AAGAACCAGG TCTCCATCCA CAGCTTCATG ACCCAACCTG AGTACGCCGC AGACGATGAA 
ATGTCACAGT CCTTCCTGAA TCAAGAGGAA AGTCCTTCGT TGGCTTCTAA GTCGCGGATT 
ACCAAGCGCC TGTCGGATGC TGAAACTGTT TCACAGAACA ACACGAATAA CAATGCCGTG 
GACTGCCACC AAGTGCAAAT CGTTGCCTCC CATCCCAACA GCCCTCTACA AAGCCAGGAG 
ACACCGGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Atp2b4 ( XM_008769448.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008767670.1 Rattus norvegicus ATPase plasma membrane Ca2+ transporting 4 (Atp2b4), transcript variant X3, mRNA.

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CloneID	ORa43326
Clone ID Related Accession (Same CDS sequence)	XM_008769448.2
Accession Version	XM_008769448.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	plasma membrane calcium-transporting ATPase 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008769448.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGAATC CATCAGGACA TAACTTGCCA GCCAACTCGG TAGCTGAGAG CTATGAAGGG 
GAGTTTGGCT GCACCTTAAT GGACCTGAGG AAGCTCATGG AGCTTCGTGC AGCTGATGCA 
GTGACCCAGA TCAGTGCCCA CTATGGAAGT GTACAAGAAA TCTGCGCAAG ACTGAAAACC 
TCCCCTGTAG AAGGTCTGTC TGGGAACCCT GCAGATTTGG AGAAGCGTAG ACTTGTTTTT 
GGGAAGAACA TGATACCTCC GAAAAAGCCC AAGACGTTCT TAGAATTAGT GTGGGAAGCC 
CTGCAGGATG TCACACTCAT CATCCTGGAG ATCGCAGCCA TCATATCCCT GGTCCTGTCC 
TTCTACCGGC CTCCTGGCGG GGAAAATGAA ATCTGTGGCC ACATTGTAAG TAACCCAGAA 
GAAGATGAGG AAGGAGAAAC TGGCTGGATT GAAGGGGCAG CCATTCTTGC CTCGGTGATC 
ATTGTGGTAT TCGTGACAGC CTTCAACGAC TGGAGCAAAG AGAAGCAGTT CCGGGGGCTG 
CAGAGTCGCA TTGAGCTTGA GCAAAAATTC TCCATTATCC GGAATGGTCA GCTTATACAA 
CTCCCAGTGG CTGAGATTGT GGTGGGAGAT ATTGCCCAGG TCAAATACGG TGACCTGCTG 
CCTGCCGACG GAATCCTAAT CCAGGGAAAT GATCTGAAGA TTGACGAGAG CTCTTTGACA 
GGAGAATCAG ATCATGTCAA GAAGACTCTG GACAAAGACC CCATGTTGCT CTCAGGGACT 
CATGTGATGG AAGGCTCTGG ACGGATGGTG GTGACTGCTG TGGGAATCAA TTCCCAGACT 
GGAATCATCT TCACCCTCTT AGGGGCTAAT GAGGAAGAAG ACGATGAGAA AAAGAAGAAA 
GCAAAAACCC AGGATGGAGT GGCCTTGGAA ATCCAGCCAC TCAACAGCCA GGAGGGGCTC 
GATAGTGAGG AAAAGGAGAA GAAAGCAAGC AAGGGACCCA AGAAGGAGAA GTCGGTCCTG 
CAGGGCAAGC TCACACGCCT GGCTGTGCAG ATCGGGAAGG CAGGTCTGAT CATGTCTATC 
CTCACAGTCC TCATCCTGAT TCTATACTTC GTGGTTGACA ATTTCGTGAT ACAGCGCCGG 
GCATGGCTTC CTGAGTGCAC TCCTGTGTAC ATCCAGTATT TTGTCAAGTT CTTCATCATC 
GGAGTCACTG TACTGGTAGT GGCTGTGCCA GAGGGGCTGC CACTGGCTGT CACCATCTCG 
TTGGCCTACT CTGTAAAGAA AATGATGAAG GACAACAACT TGGTCCGGCA CTTGGACGCC 
TGTGAGACGA TGGGCAACGC CACAGCCATC TGCTCTGATA AGACGGGCAC GCTGACCATG 
AACCGCATGA CCGTGGTACA AGCTTACATT GGGGGCACTC ACTACCGCCA GATCCCAAAA 
CCTGACGACC TCCCACCCAA CGTTCTGGAC CTCATAGTCA ACAGCATCTG TATCAACTCT 
GCTTACACTT CTAAGATTCT GCCTCCAGAG AAAGAGGGAG GCCTCCCCCG GCAAGTAGGC 
AACAAGACAG AGTGTGGACT GCTGGGCTTT GTCACAGACC TGAAGCAGGA CTACCAAGCA 
GTTCGCAGTG AGATGCCCGA GGAGAAGCTC TTCAAGGTGT ACACCTTCAA CTCAGTGCGC 
AAGTCCATGA GCACCGTCAT CAGGAAGCCG GAAGGGGGCT TCCGCGTGTT CAGCAAGGGC 
GCCTCCGAGA TAATGCTGCG CAAGTGTGAT CGGATCCTGA ACAAGGAAGG AGGAATTGTA 
CCATTCAAAA CTAAGGACCG AGACAATATG GTACGCAACG TCATTGAGCC CATGGCCAGT 
GAAGGACTCC GGACCATCGG CATAGCTTAC CGAGATTTTG ATGGTGAAGA GCCCTCGTGG 
GAAAATGAGA ATGAGATCTT CACTGGGCTG GTCTGCATTG CAGTGGTGGG CATTGAGGAC 
CCTGTGCGCC CAGAGGTACC AGATGCCATT AACAAGTGCA AACGGGCTGG CATTACTGTC 
AGGATGGTGA CAGGTGACAA CGTCAACACA GCCAGGGCCA TCGCCACCAA ATGTGGCATC 
TTGACTCCTG GTGATGACTT CCTGTGTCTG GAAGGGAAAG AATTCAACAG ACTCATACGC 
AATGAGAAGG GCGAGGTCGA ACAAGAAAAG CTGGACAAGG TCTGGCCCAG GCTTCGGGTA 
CTGGCACGAT CTTCTCCCAC AGACAAGCAC ACATTGGTGA AAGGTATCAT TGACAGCAAT 
ATTGGGGAAC AGAGGCAGGT GGTGGCTGTC ACCGGTGATG GAACCAATGA TGGACCGGCT 
CTAAAGAAAG CAGATGTCGG TTTTGCTATG GGCATTGCAG GCACAGATGT GGCTAAGGAG 
GCATCAGACA TCATCCTGAC TGATGACAAC TTCACCAGCA TTGTGAAGGC AGTGATGTGG 
GGCCGGAACG TATACGACAG CATTTCCAAG TTCCTGCAGT TCCAGCTCAC TGTCAACGTG 
GTGGCCGTGA TTGTGGCTTT CTCTGGAGCC TGCATCACTC AGGATTCCCC ACTGAAAGCG 
GTGCAGATGC TGTGGGTGAA CCTGATCATG GACACGTTTG CTTCTCTGGC CCTGGCCACA 
GAGCCCCCGA CCGATTCCCT GCTGAGGCGT CGCCCCTATG GGCGAAACAA GCCCCTGATC 
TCTCGCACAA TGATGAAGAA CATCCTGGGC CACGCGGTGT ATCAGCTCGG AATCGTCTTC 
CTCCTCGTCT TTGCTGGCGA CAAACTGTTT GACATTGACA GTGGGAGGAA GGCGCCTCTC 
AATTCACCAC CCAGCCAGCA CTACACCATT GTTTTCAACA CGTTTGTGCT GATGCAACTC 
TTCAACGAGA TCAACTCTCG GAAGATCCAT GGCGAGAAGA ATGTCTTTGC AGGTGTCTAC 
CGCAACATCA TTTTCTGCTC TGTTGTTTTA GGCACGTTCT TCTGCCAGAT CTTGATCGTG 
GAAGTGGGAG GTAAGCCCTT CAGCTGCACA AACCTCACCA TGGAGCAGTG GATGTGGTGT 
CTCTTTATAG GGATCGGAGA ACTTTTGTGG GGCCAGGTCA TCTCTGCAAT ACCTACCAAG 
TCTCTAAAGT TCCTGAAAGA GGCGGGCCAT GGCAGTGACA AAGAGGAGAT CAGCAAGGAC 
GCTGAGGGGC TGGAAGAGAT CGACCATGCC GAGATGGAGC TTCGCCGAGG CCAGATCCTC 
TGGGTCCGTG GCCTGAACCG GATTCAGACT CAGATCAGAG TGGTCAAAGT ATTTCATAGT 
TTCCGAGACG TCATTCATAA ATCCAAGAAC CAGGTCTCCA TCCACAGCTT CATGACCCAA 
CCTGAGTACG CCGCAGACGA TGAAATGTCA CAGTCCTTCC TGAATCAAGA GGAAAGTCCT 
TCGTTGGCTT CTAAGTCGCG GATTACCAAG CGCCTGTCGG ATGCTGAAAC TGTTTCACAG 
AACAACACGA ATAACAATGC CGTGGACTGC CACCAAGTGC AAATCGTTGC CTCCCATCCC 
AACAGCCCTC TACAAAGCCA GGAGACACCG GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008767670.1
CDS	546..4121
Translation	MTNPSGHNLPANSVAESYEGEFGCTLMDLRKLMELRAADAVTQISAHYGSVQEICARLKTSPVEGLSGNPADLEKRRLVFGKNMIPPKKPKTFLELVWEALQDVTLIILEIAAIISLVLSFYRPPGGENEICGHIVSNPEEDEEGETGWIEGAAILASVIIVVFVTAFNDWSKEKQFRGLQSRIELEQKFSIIRNGQLIQLPVAEIVVGDIAQVKYGDLLPADGILIQGNDLKIDESSLTGESDHVKKTLDKDPMLLSGTHVMEGSGRMVVTAVGINSQTGIIFTLLGANEEEDDEKKKKAKTQDGVALEIQPLNSQEGLDSEEKEKKASKGPKKEKSVLQGKLTRLAVQIGKAGLIMSILTVLILILYFVVDNFVIQRRAWLPECTPVYIQYFVKFFIIGVTVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTMNRMTVVQAYIGGTHYRQIPKPDDLPPNVLDLIVNSICINSAYTSKILPPEKEGGLPRQVGNKTECGLLGFVTDLKQDYQAVRSEMPEEKLFKVYTFNSVRKSMSTVIRKPEGGFRVFSKGASEIMLRKCDRILNKEGGIVPFKTKDRDNMVRNVIEPMASEGLRTIGIAYRDFDGEEPSWENENEIFTGLVCIAVVGIEDPVRPEVPDAINKCKRAGITVRMVTGDNVNTARAIATKCGILTPGDDFLCLEGKEFNRLIRNEKGEVEQEKLDKVWPRLRVLARSSPTDKHTLVKGIIDSNIGEQRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFTSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVIVAFSGACITQDSPLKAVQMLWVNLIMDTFASLALATEPPTDSLLRRRPYGRNKPLISRTMMKNILGHAVYQLGIVFLLVFAGDKLFDIDSGRKAPLNSPPSQHYTIVFNTFVLMQLFNEINSRKIHGEKNVFAGVYRNIIFCSVVLGTFFCQILIVEVGGKPFSCTNLTMEQWMWCLFIGIGELLWGQVISAIPTKSLKFLKEAGHGSDKEEISKDAEGLEEIDHAEMELRRGQILWVRGLNRIQTQIRVVKVFHSFRDVIHKSKNQVSIHSFMTQPEYAADDEMSQSFLNQEESPSLASKSRITKRLSDAETVSQNNTNNNAVDCHQVQIVASHPNSPLQSQETPV

Target ORF information:

RefSeq Version	XM_008769448.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ATPase plasma membrane Ca2+ transporting 4 (Atp2b4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008769448.2

ORF Insert Sequence:

ATGACGAATC CATCAGGACA TAACTTGCCA GCCAACTCGG TAGCTGAGAG CTATGAAGGG 
GAGTTTGGCT GCACCTTAAT GGACCTGAGG AAGCTCATGG AGCTTCGTGC AGCTGATGCA 
GTGACCCAGA TCAGTGCCCA CTATGGAAGT GTACAAGAAA TCTGCGCAAG ACTGAAAACC 
TCCCCTGTAG AAGGTCTGTC TGGGAACCCT GCAGATTTGG AGAAGCGTAG ACTTGTTTTT 
GGGAAGAACA TGATACCTCC GAAAAAGCCC AAGACGTTCT TAGAATTAGT GTGGGAAGCC 
CTGCAGGATG TCACACTCAT CATCCTGGAG ATCGCAGCCA TCATATCCCT GGTCCTGTCC 
TTCTACCGGC CTCCTGGCGG GGAAAATGAA ATCTGTGGCC ACATTGTAAG TAACCCAGAA 
GAAGATGAGG AAGGAGAAAC TGGCTGGATT GAAGGGGCAG CCATTCTTGC CTCGGTGATC 
ATTGTGGTAT TCGTGACAGC CTTCAACGAC TGGAGCAAAG AGAAGCAGTT CCGGGGGCTG 
CAGAGTCGCA TTGAGCTTGA GCAAAAATTC TCCATTATCC GGAATGGTCA GCTTATACAA 
CTCCCAGTGG CTGAGATTGT GGTGGGAGAT ATTGCCCAGG TCAAATACGG TGACCTGCTG 
CCTGCCGACG GAATCCTAAT CCAGGGAAAT GATCTGAAGA TTGACGAGAG CTCTTTGACA 
GGAGAATCAG ATCATGTCAA GAAGACTCTG GACAAAGACC CCATGTTGCT CTCAGGGACT 
CATGTGATGG AAGGCTCTGG ACGGATGGTG GTGACTGCTG TGGGAATCAA TTCCCAGACT 
GGAATCATCT TCACCCTCTT AGGGGCTAAT GAGGAAGAAG ACGATGAGAA AAAGAAGAAA 
GCAAAAACCC AGGATGGAGT GGCCTTGGAA ATCCAGCCAC TCAACAGCCA GGAGGGGCTC 
GATAGTGAGG AAAAGGAGAA GAAAGCAAGC AAGGGACCCA AGAAGGAGAA GTCGGTCCTG 
CAGGGCAAGC TCACACGCCT GGCTGTGCAG ATCGGGAAGG CAGGTCTGAT CATGTCTATC 
CTCACAGTCC TCATCCTGAT TCTATACTTC GTGGTTGACA ATTTCGTGAT ACAGCGCCGG 
GCATGGCTTC CTGAGTGCAC TCCTGTGTAC ATCCAGTATT TTGTCAAGTT CTTCATCATC 
GGAGTCACTG TACTGGTAGT GGCTGTGCCA GAGGGGCTGC CACTGGCTGT CACCATCTCG 
TTGGCCTACT CTGTAAAGAA AATGATGAAG GACAACAACT TGGTCCGGCA CTTGGACGCC 
TGTGAGACGA TGGGCAACGC CACAGCCATC TGCTCTGATA AGACGGGCAC GCTGACCATG 
AACCGCATGA CCGTGGTACA AGCTTACATT GGGGGCACTC ACTACCGCCA GATCCCAAAA 
CCTGACGACC TCCCACCCAA CGTTCTGGAC CTCATAGTCA ACAGCATCTG TATCAACTCT 
GCTTACACTT CTAAGATTCT GCCTCCAGAG AAAGAGGGAG GCCTCCCCCG GCAAGTAGGC 
AACAAGACAG AGTGTGGACT GCTGGGCTTT GTCACAGACC TGAAGCAGGA CTACCAAGCA 
GTTCGCAGTG AGATGCCCGA GGAGAAGCTC TTCAAGGTGT ACACCTTCAA CTCAGTGCGC 
AAGTCCATGA GCACCGTCAT CAGGAAGCCG GAAGGGGGCT TCCGCGTGTT CAGCAAGGGC 
GCCTCCGAGA TAATGCTGCG CAAGTGTGAT CGGATCCTGA ACAAGGAAGG AGGAATTGTA 
CCATTCAAAA CTAAGGACCG AGACAATATG GTACGCAACG TCATTGAGCC CATGGCCAGT 
GAAGGACTCC GGACCATCGG CATAGCTTAC CGAGATTTTG ATGGTGAAGA GCCCTCGTGG 
GAAAATGAGA ATGAGATCTT CACTGGGCTG GTCTGCATTG CAGTGGTGGG CATTGAGGAC 
CCTGTGCGCC CAGAGGTACC AGATGCCATT AACAAGTGCA AACGGGCTGG CATTACTGTC 
AGGATGGTGA CAGGTGACAA CGTCAACACA GCCAGGGCCA TCGCCACCAA ATGTGGCATC 
TTGACTCCTG GTGATGACTT CCTGTGTCTG GAAGGGAAAG AATTCAACAG ACTCATACGC 
AATGAGAAGG GCGAGGTCGA ACAAGAAAAG CTGGACAAGG TCTGGCCCAG GCTTCGGGTA 
CTGGCACGAT CTTCTCCCAC AGACAAGCAC ACATTGGTGA AAGGTATCAT TGACAGCAAT 
ATTGGGGAAC AGAGGCAGGT GGTGGCTGTC ACCGGTGATG GAACCAATGA TGGACCGGCT 
CTAAAGAAAG CAGATGTCGG TTTTGCTATG GGCATTGCAG GCACAGATGT GGCTAAGGAG 
GCATCAGACA TCATCCTGAC TGATGACAAC TTCACCAGCA TTGTGAAGGC AGTGATGTGG 
GGCCGGAACG TATACGACAG CATTTCCAAG TTCCTGCAGT TCCAGCTCAC TGTCAACGTG 
GTGGCCGTGA TTGTGGCTTT CTCTGGAGCC TGCATCACTC AGGATTCCCC ACTGAAAGCG 
GTGCAGATGC TGTGGGTGAA CCTGATCATG GACACGTTTG CTTCTCTGGC CCTGGCCACA 
GAGCCCCCGA CCGATTCCCT GCTGAGGCGT CGCCCCTATG GGCGAAACAA GCCCCTGATC 
TCTCGCACAA TGATGAAGAA CATCCTGGGC CACGCGGTGT ATCAGCTCGG AATCGTCTTC 
CTCCTCGTCT TTGCTGGCGA CAAACTGTTT GACATTGACA GTGGGAGGAA GGCGCCTCTC 
AATTCACCAC CCAGCCAGCA CTACACCATT GTTTTCAACA CGTTTGTGCT GATGCAACTC 
TTCAACGAGA TCAACTCTCG GAAGATCCAT GGCGAGAAGA ATGTCTTTGC AGGTGTCTAC 
CGCAACATCA TTTTCTGCTC TGTTGTTTTA GGCACGTTCT TCTGCCAGAT CTTGATCGTG 
GAAGTGGGAG GTAAGCCCTT CAGCTGCACA AACCTCACCA TGGAGCAGTG GATGTGGTGT 
CTCTTTATAG GGATCGGAGA ACTTTTGTGG GGCCAGGTCA TCTCTGCAAT ACCTACCAAG 
TCTCTAAAGT TCCTGAAAGA GGCGGGCCAT GGCAGTGACA AAGAGGAGAT CAGCAAGGAC 
GCTGAGGGGC TGGAAGAGAT CGACCATGCC GAGATGGAGC TTCGCCGAGG CCAGATCCTC 
TGGGTCCGTG GCCTGAACCG GATTCAGACT CAGATCAGAG TGGTCAAAGT ATTTCATAGT 
TTCCGAGACG TCATTCATAA ATCCAAGAAC CAGGTCTCCA TCCACAGCTT CATGACCCAA 
CCTGAGTACG CCGCAGACGA TGAAATGTCA CAGTCCTTCC TGAATCAAGA GGAAAGTCCT 
TCGTTGGCTT CTAAGTCGCG GATTACCAAG CGCCTGTCGG ATGCTGAAAC TGTTTCACAG 
AACAACACGA ATAACAATGC CGTGGACTGC CACCAAGTGC AAATCGTTGC CTCCCATCCC 
AACAGCCCTC TACAAAGCCA GGAGACACCG GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Atp2b4 ( XM_008769450.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008767672.1 Rattus norvegicus ATPase plasma membrane Ca2+ transporting 4 (Atp2b4), transcript variant X5, mRNA.

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CloneID	ORa43327
Clone ID Related Accession (Same CDS sequence)	XM_008769450.2
Accession Version	XM_008769450.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	plasma membrane calcium-transporting ATPase 4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008769450.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGAATC CATCAGGACA TAACTTGCCA GCCAACTCGG TAGCTGAGAG CTATGAAGGG 
GAGTTTGGCT GCACCTTAAT GGACCTGAGG AAGCTCATGG AGCTTCGTGC AGCTGATGCA 
GTGACCCAGA TCAGTGCCCA CTATGGAAGT GTACAAGAAA TCTGCGCAAG ACTGAAAACC 
TCCCCTGTAG AAGGTCTGTC TGGGAACCCT GCAGATTTGG AGAAGCGTAG ACTTGTTTTT 
GGGAAGAACA TGATACCTCC GAAAAAGCCC AAGACGTTCT TAGAATTAGT GTGGGAAGCC 
CTGCAGGATG TCACACTCAT CATCCTGGAG ATCGCAGCCA TCATATCCCT GGTCCTGTCC 
TTCTACCGGC CTCCTGGCGG GGAAAATGAA ATCTGTGGCC ACATTGTAAG TAACCCAGAA 
GAAGATGAGG AAGGAGAAAC TGGCTGGATT GAAGGGGCAG CCATTCTTGC CTCGGTGATC 
ATTGTGGTAT TCGTGACAGC CTTCAACGAC TGGAGCAAAG AGAAGCAGTT CCGGGGGCTG 
CAGAGTCGCA TTGAGCTTGA GCAAAAATTC TCCATTATCC GGAATGGTCA GCTTATACAA 
CTCCCAGTGG CTGAGATTGT GGTGGGAGAT ATTGCCCAGG TCAAATACGG TGACCTGCTG 
CCTGCCGACG GAATCCTAAT CCAGGGAAAT GATCTGAAGA TTGACGAGAG CTCTTTGACA 
GGAGAATCAG ATCATGTCAA GAAGACTCTG GACAAAGACC CCATGTTGCT CTCAGGGACT 
CATGTGATGG AAGGCTCTGG ACGGATGGTG GTGACTGCTG TGGGAATCAA TTCCCAGACT 
GGAATCATCT TCACCCTCTT AGGGGCTAAT GAGGAAGAAG ACGATGAGAA AAAGAAGAAA 
GCAAAAACCC AGGATGGAGT GGCCTTGGAA ATCCAGCCAC TCAACAGCCA GGAGGGGCTC 
GATAGTGAGG AAAAGGAGAA GAAAGCAAGC AAGGGACCCA AGAAGGAGAA GTCGGTCCTG 
CAGGGCAAGC TCACACGCCT GGCTGTGCAG ATCGGGAAGG CAGGTCTGAT CATGTCTATC 
CTCACAGTCC TCATCCTGAT TCTATACTTC GTGGTTGACA ATTTCGTGAT ACAGCGCCGG 
GCATGGCTTC CTGAGTGCAC TCCTGTGTAC ATCCAGTATT TTGTCAAGTT CTTCATCATC 
GGAGTCACTG TACTGGTAGT GGCTGTGCCA GAGGGGCTGC CACTGGCTGT CACCATCTCG 
TTGGCCTACT CTGTAAAGAA AATGATGAAG GACAACAACT TGGTCCGGCA CTTGGACGCC 
TGTGAGACGA TGGGCAACGC CACAGCCATC TGCTCTGATA AGACGGGCAC GCTGACCATG 
AACCGCATGA CCGTGGTACA AGCTTACATT GGGGGCACTC ACTACCGCCA GATCCCAAAA 
CCTGACGACC TCCCACCCAA CGTTCTGGAC CTCATAGTCA ACAGCATCTG TATCAACTCT 
GCTTACACTT CTAAGATTCT GCCTCCAGAG AAAGAGGGAG GCCTCCCCCG GCAAGTAGGC 
AACAAGACAG AGTGTGGACT GCTGGGCTTT GTCACAGACC TGAAGCAGGA CTACCAAGCA 
GTTCGCAGTG AGATGCCCGA GGAGAAGCTC TTCAAGGTGT ACACCTTCAA CTCAGTGCGC 
AAGTCCATGA GCACCGTCAT CAGGAAGCCG GAAGGGGGCT TCCGCGTGTT CAGCAAGGGC 
GCCTCCGAGA TAATGCTGCG CAAGTGTGAT CGGATCCTGA ACAAGGAAGG AGGAATTGTA 
CCATTCAAAA CTAAGGACCG AGACAATATG GTACGCAACG TCATTGAGCC CATGGCCAGT 
GAAGGACTCC GGACCATCGG CATAGCTTAC CGAGATTTTG ATGGTGAAGA GCCCTCGTGG 
GAAAATGAGA ATGAGATCTT CACTGGGCTG GTCTGCATTG CAGTGGTGGG CATTGAGGAC 
CCTGTGCGCC CAGAGGTACC AGATGCCATT AACAAGTGCA AACGGGCTGG CATTACTGTC 
AGGATGGTGA CAGGTGACAA CGTCAACACA GCCAGGGCCA TCGCCACCAA ATGTGGCATC 
TTGACTCCTG GTGATGACTT CCTGTGTCTG GAAGGGAAAG AATTCAACAG ACTCATACGC 
AATGAGAAGG GCGAGGTCGA ACAAGAAAAG CTGGACAAGG TCTGGCCCAG GCTTCGGGTA 
CTGGCACGAT CTTCTCCCAC AGACAAGCAC ACATTGGTGA AAGGTATCAT TGACAGCAAT 
ATTGGGGAAC AGAGGCAGGT GGTGGCTGTC ACCGGTGATG GAACCAATGA TGGACCGGCT 
CTAAAGAAAG CAGATGTCGG TTTTGCTATG GGCATTGCAG GCACAGATGT GGCTAAGGAG 
GCATCAGACA TCATCCTGAC TGATGACAAC TTCACCAGCA TTGTGAAGGC AGTGATGTGG 
GGCCGGAACG TATACGACAG CATTTCCAAG TTCCTGCAGT TCCAGCTCAC TGTCAACGTG 
GTGGCCGTGA TTGTGGCTTT CTCTGGAGCC TGCATCACTC AGGATTCCCC ACTGAAAGCG 
GTGCAGATGC TGTGGGTGAA CCTGATCATG GACACGTTTG CTTCTCTGGC CCTGGCCACA 
GAGCCCCCGA CCGATTCCCT GCTGAGGCGT CGCCCCTATG GGCGAAACAA GCCCCTGATC 
TCTCGCACAA TGATGAAGAA CATCCTGGGC CACGCGGTGT ATCAGCTCGG AATCGTCTTC 
CTCCTCGTCT TTGCTGGCGA CAAACTGTTT GACATTGACA GTGGGAGGAA GGCGCCTCTC 
AATTCACCAC CCAGCCAGCA CTACACCATT GTTTTCAACA CGTTTGTGCT GATGCAACTC 
TTCAACGAGA TCAACTCTCG GAAGATCCAT GGCGAGAAGA ATGTCTTTGC AGGTGTCTAC 
CGCAACATCA TTTTCTGCTC TGTTGTTTTA GGCACGTTCT TCTGCCAGAT CTTGATCGTG 
GAAGTGGGAG GTAAGCCCTT CAGCTGCACA AACCTCACCA TGGAGCAGTG GATGTGGTGT 
CTCTTTATAG GGATCGGAGA ACTTTTGTGG GGCCAGGTCA TCTCTGCAAT ACCTACCAAG 
TCTCTAAAGT TCCTGAAAGA GGCGGGCCAT GGCAGTGACA AAGAGGAGAT CAGCAAGGAC 
GCTGAGGGGC TGGAAGAGAT CGACCATGCC GAGATGGAGC TTCGCCGAGG CCAGATCCTC 
TGGGTCCGTG GCCTGAACCG GATTCAGACT CAGATTGAAG TAATTAACAA ATTCCAGACG 
GGAGCCTCTT TTAAGGGAGT CCTAAGGCGA CAGAACTTGA GTCAACAACT TGATGTAAAA 
CTGGTTCCTA GCTCCTATTC AGAAGCAGTT GCGTCAGTCA GAACCTCCCC CTCCACCTCT 
TCTGCCGTTA CACCTCCTCC TGTGGGCAAT CAGAGTGGTC AAAGTATTTC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008767672.1
CDS	547..4020
Translation	MTNPSGHNLPANSVAESYEGEFGCTLMDLRKLMELRAADAVTQISAHYGSVQEICARLKTSPVEGLSGNPADLEKRRLVFGKNMIPPKKPKTFLELVWEALQDVTLIILEIAAIISLVLSFYRPPGGENEICGHIVSNPEEDEEGETGWIEGAAILASVIIVVFVTAFNDWSKEKQFRGLQSRIELEQKFSIIRNGQLIQLPVAEIVVGDIAQVKYGDLLPADGILIQGNDLKIDESSLTGESDHVKKTLDKDPMLLSGTHVMEGSGRMVVTAVGINSQTGIIFTLLGANEEEDDEKKKKAKTQDGVALEIQPLNSQEGLDSEEKEKKASKGPKKEKSVLQGKLTRLAVQIGKAGLIMSILTVLILILYFVVDNFVIQRRAWLPECTPVYIQYFVKFFIIGVTVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTMNRMTVVQAYIGGTHYRQIPKPDDLPPNVLDLIVNSICINSAYTSKILPPEKEGGLPRQVGNKTECGLLGFVTDLKQDYQAVRSEMPEEKLFKVYTFNSVRKSMSTVIRKPEGGFRVFSKGASEIMLRKCDRILNKEGGIVPFKTKDRDNMVRNVIEPMASEGLRTIGIAYRDFDGEEPSWENENEIFTGLVCIAVVGIEDPVRPEVPDAINKCKRAGITVRMVTGDNVNTARAIATKCGILTPGDDFLCLEGKEFNRLIRNEKGEVEQEKLDKVWPRLRVLARSSPTDKHTLVKGIIDSNIGEQRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFTSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVIVAFSGACITQDSPLKAVQMLWVNLIMDTFASLALATEPPTDSLLRRRPYGRNKPLISRTMMKNILGHAVYQLGIVFLLVFAGDKLFDIDSGRKAPLNSPPSQHYTIVFNTFVLMQLFNEINSRKIHGEKNVFAGVYRNIIFCSVVLGTFFCQILIVEVGGKPFSCTNLTMEQWMWCLFIGIGELLWGQVISAIPTKSLKFLKEAGHGSDKEEISKDAEGLEEIDHAEMELRRGQILWVRGLNRIQTQIEVINKFQTGASFKGVLRRQNLSQQLDVKLVPSSYSEAVASVRTSPSTSSAVTPPPVGNQSGQSIS

Target ORF information:

RefSeq Version	XM_008769450.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ATPase plasma membrane Ca2+ transporting 4 (Atp2b4), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008769450.2

ORF Insert Sequence:

ATGACGAATC CATCAGGACA TAACTTGCCA GCCAACTCGG TAGCTGAGAG CTATGAAGGG 
GAGTTTGGCT GCACCTTAAT GGACCTGAGG AAGCTCATGG AGCTTCGTGC AGCTGATGCA 
GTGACCCAGA TCAGTGCCCA CTATGGAAGT GTACAAGAAA TCTGCGCAAG ACTGAAAACC 
TCCCCTGTAG AAGGTCTGTC TGGGAACCCT GCAGATTTGG AGAAGCGTAG ACTTGTTTTT 
GGGAAGAACA TGATACCTCC GAAAAAGCCC AAGACGTTCT TAGAATTAGT GTGGGAAGCC 
CTGCAGGATG TCACACTCAT CATCCTGGAG ATCGCAGCCA TCATATCCCT GGTCCTGTCC 
TTCTACCGGC CTCCTGGCGG GGAAAATGAA ATCTGTGGCC ACATTGTAAG TAACCCAGAA 
GAAGATGAGG AAGGAGAAAC TGGCTGGATT GAAGGGGCAG CCATTCTTGC CTCGGTGATC 
ATTGTGGTAT TCGTGACAGC CTTCAACGAC TGGAGCAAAG AGAAGCAGTT CCGGGGGCTG 
CAGAGTCGCA TTGAGCTTGA GCAAAAATTC TCCATTATCC GGAATGGTCA GCTTATACAA 
CTCCCAGTGG CTGAGATTGT GGTGGGAGAT ATTGCCCAGG TCAAATACGG TGACCTGCTG 
CCTGCCGACG GAATCCTAAT CCAGGGAAAT GATCTGAAGA TTGACGAGAG CTCTTTGACA 
GGAGAATCAG ATCATGTCAA GAAGACTCTG GACAAAGACC CCATGTTGCT CTCAGGGACT 
CATGTGATGG AAGGCTCTGG ACGGATGGTG GTGACTGCTG TGGGAATCAA TTCCCAGACT 
GGAATCATCT TCACCCTCTT AGGGGCTAAT GAGGAAGAAG ACGATGAGAA AAAGAAGAAA 
GCAAAAACCC AGGATGGAGT GGCCTTGGAA ATCCAGCCAC TCAACAGCCA GGAGGGGCTC 
GATAGTGAGG AAAAGGAGAA GAAAGCAAGC AAGGGACCCA AGAAGGAGAA GTCGGTCCTG 
CAGGGCAAGC TCACACGCCT GGCTGTGCAG ATCGGGAAGG CAGGTCTGAT CATGTCTATC 
CTCACAGTCC TCATCCTGAT TCTATACTTC GTGGTTGACA ATTTCGTGAT ACAGCGCCGG 
GCATGGCTTC CTGAGTGCAC TCCTGTGTAC ATCCAGTATT TTGTCAAGTT CTTCATCATC 
GGAGTCACTG TACTGGTAGT GGCTGTGCCA GAGGGGCTGC CACTGGCTGT CACCATCTCG 
TTGGCCTACT CTGTAAAGAA AATGATGAAG GACAACAACT TGGTCCGGCA CTTGGACGCC 
TGTGAGACGA TGGGCAACGC CACAGCCATC TGCTCTGATA AGACGGGCAC GCTGACCATG 
AACCGCATGA CCGTGGTACA AGCTTACATT GGGGGCACTC ACTACCGCCA GATCCCAAAA 
CCTGACGACC TCCCACCCAA CGTTCTGGAC CTCATAGTCA ACAGCATCTG TATCAACTCT 
GCTTACACTT CTAAGATTCT GCCTCCAGAG AAAGAGGGAG GCCTCCCCCG GCAAGTAGGC 
AACAAGACAG AGTGTGGACT GCTGGGCTTT GTCACAGACC TGAAGCAGGA CTACCAAGCA 
GTTCGCAGTG AGATGCCCGA GGAGAAGCTC TTCAAGGTGT ACACCTTCAA CTCAGTGCGC 
AAGTCCATGA GCACCGTCAT CAGGAAGCCG GAAGGGGGCT TCCGCGTGTT CAGCAAGGGC 
GCCTCCGAGA TAATGCTGCG CAAGTGTGAT CGGATCCTGA ACAAGGAAGG AGGAATTGTA 
CCATTCAAAA CTAAGGACCG AGACAATATG GTACGCAACG TCATTGAGCC CATGGCCAGT 
GAAGGACTCC GGACCATCGG CATAGCTTAC CGAGATTTTG ATGGTGAAGA GCCCTCGTGG 
GAAAATGAGA ATGAGATCTT CACTGGGCTG GTCTGCATTG CAGTGGTGGG CATTGAGGAC 
CCTGTGCGCC CAGAGGTACC AGATGCCATT AACAAGTGCA AACGGGCTGG CATTACTGTC 
AGGATGGTGA CAGGTGACAA CGTCAACACA GCCAGGGCCA TCGCCACCAA ATGTGGCATC 
TTGACTCCTG GTGATGACTT CCTGTGTCTG GAAGGGAAAG AATTCAACAG ACTCATACGC 
AATGAGAAGG GCGAGGTCGA ACAAGAAAAG CTGGACAAGG TCTGGCCCAG GCTTCGGGTA 
CTGGCACGAT CTTCTCCCAC AGACAAGCAC ACATTGGTGA AAGGTATCAT TGACAGCAAT 
ATTGGGGAAC AGAGGCAGGT GGTGGCTGTC ACCGGTGATG GAACCAATGA TGGACCGGCT 
CTAAAGAAAG CAGATGTCGG TTTTGCTATG GGCATTGCAG GCACAGATGT GGCTAAGGAG 
GCATCAGACA TCATCCTGAC TGATGACAAC TTCACCAGCA TTGTGAAGGC AGTGATGTGG 
GGCCGGAACG TATACGACAG CATTTCCAAG TTCCTGCAGT TCCAGCTCAC TGTCAACGTG 
GTGGCCGTGA TTGTGGCTTT CTCTGGAGCC TGCATCACTC AGGATTCCCC ACTGAAAGCG 
GTGCAGATGC TGTGGGTGAA CCTGATCATG GACACGTTTG CTTCTCTGGC CCTGGCCACA 
GAGCCCCCGA CCGATTCCCT GCTGAGGCGT CGCCCCTATG GGCGAAACAA GCCCCTGATC 
TCTCGCACAA TGATGAAGAA CATCCTGGGC CACGCGGTGT ATCAGCTCGG AATCGTCTTC 
CTCCTCGTCT TTGCTGGCGA CAAACTGTTT GACATTGACA GTGGGAGGAA GGCGCCTCTC 
AATTCACCAC CCAGCCAGCA CTACACCATT GTTTTCAACA CGTTTGTGCT GATGCAACTC 
TTCAACGAGA TCAACTCTCG GAAGATCCAT GGCGAGAAGA ATGTCTTTGC AGGTGTCTAC 
CGCAACATCA TTTTCTGCTC TGTTGTTTTA GGCACGTTCT TCTGCCAGAT CTTGATCGTG 
GAAGTGGGAG GTAAGCCCTT CAGCTGCACA AACCTCACCA TGGAGCAGTG GATGTGGTGT 
CTCTTTATAG GGATCGGAGA ACTTTTGTGG GGCCAGGTCA TCTCTGCAAT ACCTACCAAG 
TCTCTAAAGT TCCTGAAAGA GGCGGGCCAT GGCAGTGACA AAGAGGAGAT CAGCAAGGAC 
GCTGAGGGGC TGGAAGAGAT CGACCATGCC GAGATGGAGC TTCGCCGAGG CCAGATCCTC 
TGGGTCCGTG GCCTGAACCG GATTCAGACT CAGATTGAAG TAATTAACAA ATTCCAGACG 
GGAGCCTCTT TTAAGGGAGT CCTAAGGCGA CAGAACTTGA GTCAACAACT TGATGTAAAA 
CTGGTTCCTA GCTCCTATTC AGAAGCAGTT GCGTCAGTCA GAACCTCCCC CTCCACCTCT 
TCTGCCGTTA CACCTCCTCC TGTGGGCAAT CAGAGTGGTC AAAGTATTTC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Calcium efflux activity of plasma membrane Ca2+ ATPase-4 (PMCA4) mediates cell cycle progression in vascular smooth muscle cells.
The Journal of biological chemistry289(10)7221-31(2014 Mar)
Afroze T,Yang G,Khoshbin A,Tanwir M,Tabish T,Momen A,Husain M

Molecular profiling of synaptic vesicle docking sites reveals novel proteins but few differences between glutamatergic and GABAergic synapses.
Neuron78(2)285-97(2013 Apr)
Boyken J,Gr?nborg M,Riedel D,Urlaub H,Jahn R,Chua JJ

Interaction of plasma membrane Ca(2+)-ATPase isoform 4 with calcineurin A: implications for catecholamine secretion by PC12 cells.
Biochemical and biophysical research communications411(2)235-40(2011 Jul)
Kosiorek M,Podszywalow-Bartnicka P,Zylinska L,Zablocki K,Pikula S

Defining the membrane proteome of NK cells.
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)
Ghosh D,Lippert D,Krokhin O,Cortens JP,Wilkins JA

Mutants in the putative nucleotide-binding region of the plasma membrane Ca(2+)-pump. A reduction in activity due to slow dephosphorylation.
The Journal of biological chemistry270(50)30111-4(1995 Dec)
Adamo HP,Filoteo AG,Enyedi A,Penniston JT

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Atp2b4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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