ATP2B4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ATP2B4
Entrez Gene ID	493
Full Name	ATPase plasma membrane Ca2+ transporting 4
Synonyms	ATP2B2,MXRA1,PMCA4,PMCA4b,PMCA4x
General protein information	Preferred Names ATPase plasma membrane Ca2+ transporting 4 Names plasma membrane calcium-transporting ATPase 4 ATPase, Ca++ transporting, plasma membrane 4 matrix-remodeling-associated protein 1 sarcolemmal calcium pump
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q32.1
Summary	The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 4. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008].
Disorder MIM:	108732

mRNA and Protein(s)

mRNA	Protein	Name
NM_001684.4	NP_001675.3	plasma membrane calcium-transporting ATPase 4 isoform 4b
NM_001684.5	NP_001675.3	plasma membrane calcium-transporting ATPase 4 isoform 4b
NM_001365784.1	NP_001352713.1	plasma membrane calcium-transporting ATPase 4 isoform 4c
NM_001365783.1	NP_001352712.1	plasma membrane calcium-transporting ATPase 4 isoform 4c
NM_001365783.2	NP_001352712.1	plasma membrane calcium-transporting ATPase 4 isoform 4c
NM_001001396.2	NP_001001396.1	plasma membrane calcium-transporting ATPase 4 isoform 4a

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ATP2B4	XP_005640818.1
Arabidopsis thaliana (thale cress)	ACA10	NP_194719.1
	ATP2B4	NP_001028098.1
Mus musculus (house mouse)	Atp2b4	NP_998781.1
Rattus norvegicus (Norway rat)	Atp2b4	NP_001005871.1
Magnaporthe oryzae (rice blast fungus)	MGG_04890	XP_003712375.1
Schizosaccharomyces pombe (fission yeast)	SPAPB2B4.04c	NP_593890.1
Arabidopsis thaliana (thale cress)	ACA8	NP_200521.3
Oryza sativa (rice)	Os04g0605500	NP_001053795.1
Homo sapiens (human)	ATP2B4	NP_001675.3
Gallus gallus (chicken)	ATP2B4	XP_004948503.1
Arabidopsis thaliana (thale cress)	ACA9	NP_188755.2
Xenopus tropicalis (tropical clawed frog)	atp2b4	XP_004910734.1
Pan troglodytes (chimpanzee)	ATP2B4	XP_001156333.2
Bos taurus (cattle)	ATP2B4	NP_001166065.1
Danio rerio (zebrafish)	atp2b1b	NP_001129103.1
Neurospora crassa	NCU05154	XP_956652.1

Gene Ontology
Bibliography

Related articles in PubMed

The serum protein renalase reduces injury in experimental pancreatitis.
Kolodecik TR, Reed AM, Date K, Shugrue CA, Patel V, Chung SL, Desir GV, Gorelick FS
The Journal of biological chemistry292(51)21047-21059(2017 Dec)

Sperm Na
Lestari SW, Miati DN, Seoharso P, Sugiyanto R, Pujianto DA
Systems biology in reproductive medicine63(5)294-302(2017 Oct)

Selective inhibition of plasma membrane calcium ATPase 4 improves angiogenesis and vascular reperfusion.
Kurusamy S, L?pez-Maderuelo D, Little R, Cadagan D, Savage AM, Ihugba JC, Baggott RR, Rowther FB, Mart?nez-Mart?nez S, Arco PG, Murcott C, Wang W, Francisco Nistal J, Oceandy D, Neyses L, Wilkinson RN, Cartwright EJ, Redondo JM, Armesilla AL
Journal of molecular and cellular cardiology10938-47(2017 Aug)

An erythroid-specific ATP2B4 enhancer mediates red blood cell hydration and malaria susceptibility.
Lessard S, Gatof ES, Beaudoin M, Schupp PG, Sher F, Ali A, Prehar S, Kurita R, Nakamura Y, Baena E, Ledoux J, Oceandy D, Bauer DE, Lettre G
The Journal of clinical investigation127(8)3065-3074(2017 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the ATP2B4 enhancer mediates red blood cell hydration and malaria susceptibility
Title: An erythroid-specific ATP2B4 enhancer mediates red blood cell hydration and malaria susceptibility.

Increased plasma membrane abundance of PMCA4b in vemurafenib-treated BRAF mutant cells is associated with enhanced Ca2+ clearance.
Title: The plasma membrane Ca

The role of heterozygous variants of ATP2B4 is regulated by HSPG2 protein with transcription factors in bone formation through modulation of calcium signaling.
Title: Exome sequencing identified rare variants in genes HSPG2 and ATP2B4 in a family segregating developmental dysplasia of the hip.

RNA sequencing identified a novel ATPase, Ca2+ transporting, plasma membrane 4(ATP2B4)-protein kinase C-alpha (PRKCA) fusion transcript.
Title: Pigment-Synthesizing Melanocytic Neoplasm With Protein Kinase C Alpha (PRKCA) Fusion.

our data show that CD147 interacts via its immunomodulatory domains with PMCA4 to bypass TCR proximal signaling and inhibit IL-2 expression.
Title: Association of CD147 and Calcium Exporter PMCA4 Uncouples IL-2 Expression from Early TCR Signaling.

The following ATP2B4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ATP2B4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu14814	NM_001684.5 Latest version!	Homo sapiens ATPase plasma membrane Ca2+ transporting 4 (ATP2B4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
OHu25104	NM_001001396.2 Latest version!	Homo sapiens ATPase plasma membrane Ca2+ transporting 4 (ATP2B4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
OHu107180	NM_001365783.2 Latest version!	Homo sapiens ATPase plasma membrane Ca2+ transporting 4 (ATP2B4), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu107180	NM_001365784.1 Latest version!	Homo sapiens ATPase plasma membrane Ca2+ transporting 4 (ATP2B4), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OHu14814	NM_001684.4	Homo sapiens ATPase plasma membrane Ca2+ transporting 4 (ATP2B4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
OHu107180	NM_001365783.1	Homo sapiens ATPase plasma membrane Ca2+ transporting 4 (ATP2B4), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ATP2B4 ( NM_001684.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001675.3 Homo sapiens ATPase plasma membrane Ca2+ transporting 4 (ATP2B4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu14814
Clone ID Related Accession (Same CDS sequence)	NM_001684.4 , NM_001684.5
Accession Version	NM_001684.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	plasma membrane calcium-transporting ATPase 4 isoform 4b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX537444.1, AL706212.1, DN997129.1, M25874.1, BF985985.1, AC114402.2 and AW888226.1. On Jul 19, 2011 this sequence version replaced NM_001684.3. Transcript Variant: This variant (2) lacks an alternate coding exon compared to variant 1, that causes a frameshift. The resulting isoform (4b) has a longer and distinct C-terminus compared to isoform 4a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX537444.1, CR936708.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACGAACC CATCAGACCG TGTCTTGCCT GCCAACTCGA TGGCCGAGAG CCGTGAAGGG 
GACTTTGGCT GCACAGTAAT GGAACTGAGG AAGCTCATGG AGCTGCGTTC AAGGGATGCA 
CTGACCCAGA TTAATGTCCA CTATGGAGGT GTACAGAATC TCTGCAGTAG ACTGAAAACC 
TCCCCTGTGG AAGGTCTGTC TGGGAACCCT GCAGATCTGG AGAAACGTAG GCAGGTGTTT 
GGACACAACG TGATCCCCCC CAAAAAGCCC AAGACTTTCT TAGAATTAGT GTGGGAAGCT 
CTTCAAGATG TCACGCTTAT CATCCTGGAG ATTGCAGCCA TCATCTCCCT GGTCCTGTCC 
TTTTATCGCC CTGCTGGTGA AGAAAATGAA CTGTGTGGTC AAGTCGCAAC TACCCCAGAA 
GATGAAAATG AGGCACAAGC TGGCTGGATT GAGGGGGCAG CCATCCTTTT CTCAGTGATC 
ATCGTGGTGT TAGTGACTGC CTTTAATGAT TGGAGCAAAG AGAAGCAATT CCGGGGGCTG 
CAGTGCCGCA TTGAACAGGA GCAAAAGTTC TCCATCATCC GAAACGGTCA ACTCATCCAG 
CTCCCTGTGG CTGAGATTGT GGTTGGTGAT ATTGCCCAAG TCAAATACGG TGATCTGCTG 
CCTGCAGATG GAATCCTGAT CCAAGGGAAT GATCTGAAGA TTGATGAGAG CTCTCTGACA 
GGGGAATCTG ACCATGTCAA GAAGTCCCTG GACAAAGACC CCATGTTGCT CTCAGGGACC 
CATGTCATGG AAGGTTCTGG CCGGATGGTG GTGACAGCTG TTGGTGTCAA CTCTCAGACT 
GGAATCATCC TTACTCTCTT GGGGGTCAAT GAGGATGACG AAGGGGAGAA AAAGAAGAAA 
GGTAAAAAAC AAGGAGTCCC TGAAAATCGC AACAAAGCAA AGACCCAAGA CGGAGTGGCC 
CTGGAAATCC AGCCACTCAA CAGCCAGGAG GGAATCGACA ATGAGGAAAA GGACAAGAAG 
GCAGTCAAGG TGCCTAAAAA GGAGAAGTCA GTGCTGCAGG GCAAGCTGAC TCGCCTGGCT 
GTTCAGATTG GGAAAGCCGG TCTGCTCATG TCTGCTCTCA CGGTTTTCAT CCTGATTCTA 
TACTTTGTGA TTGACAACTT TGTGATAAAT CGCAGACCAT GGCTCCCTGA GTGTACTCCC 
ATCTACATCC AGTACTTTGT CAAGTTCTTC ATCATCGGCA TCACTGTACT GGTGGTGGCT 
GTGCCAGAGG GGCTGCCTCT GGCTGTCACC ATCTCACTGG CCTACTCTGT GAAGAAAATG 
ATGAAAGACA ATAACCTAGT ACGGCACTTG GATGCTTGTG AGACCATGGG CAACGCCACC 
GCCATCTGCT CTGATAAGAC AGGCACGTTG ACCATGAACC GCATGACTGT GGTACAAGCT 
TATATTGGGG GCATCCATTA CCGTCAAATC CCAAGCCCTG ATGTCTTCCT GCCCAAAGTC 
CTGGACCTCA TTGTCAATGG CATTTCTATC AACAGTGCTT ATACCTCCAA GATTCTGCCT 
CCAGAGAAGG AGGGAGGCCT GCCTCGGCAG GTGGGCAACA AGACCGAGTG TGCTCTGCTA 
GGCTTTGTCA CAGATCTGAA GCAGGATTAT CAGGCTGTGC GTAATGAAGT GCCCGAGGAG 
AAGCTCTACA AGGTGTACAC CTTTAACTCA GTGCGCAAGT CAATGAGCAC CGTCATCAGG 
AATCCCAACG GTGGCTTCCG TATGTACAGC AAGGGCGCCT CTGAGATCAT CTTGCGCAAG 
TGTAATCGAA TCCTGGACCG GAAAGGGGAA GCAGTGCCAT TCAAGAATAA AGACAGAGAT 
GATATGGTAC GCACTGTCAT CGAGCCCATG GCCTGTGATG GACTCCGGAC TATCTGCATA 
GCTTACCGGG ACTTCGATGA CACAGAGCCC TCTTGGGACA ATGAGAATGA GATCCTCACC 
GAACTGACCT GTATCGCGGT GGTGGGCATT GAGGACCCTG TGCGCCCAGA GGTGCCAGAT 
GCTATTGCCA AATGCAAACA AGCTGGCATT ACTGTCAGAA TGGTGACAGG TGACAACATC 
AACACAGCCC GGGCCATTGC CACCAAATGT GGCATTCTGA CACCTGGGGA TGACTTCCTG 
TGCTTAGAAG GCAAAGAATT CAACCGGCTC ATCCGCAACG AGAAAGGCGA GGTAGAGCAA 
GAAAAGCTGG ACAAGATCTG GCCTAAGCTT CGGGTCCTGG CGCGATCTTC TCCCACTGAC 
AAGCACACCC TGGTGAAAGG CATAATTGAC AGCACTGTTG GGGAACACCG GCAGGTCGTG 
GCTGTCACTG GTGATGGCAC AAATGACGGG CCTGCTCTGA AGAAAGCGGA TGTTGGTTTT 
GCCATGGGCA TCGCAGGCAC AGATGTAGCA AAGGAGGCTT CAGACATCAT CCTAACAGAT 
GACAACTTCA CCAGCATTGT GAAGGCAGTG ATGTGGGGAC GAAATGTCTA TGACAGCATC 
TCCAAGTTCC TGCAGTTCCA GCTCACTGTC AATGTGGTGG CCGTGATTGT AGCCTTCACT 
GGAGCCTGTA TCACTCAGGA TTCCCCATTG AAAGCTGTGC AGATGTTGTG GGTTAATCTG 
ATCATGGACA CTTTTGCTTC ATTGGCCCTG GCCACAGAGC CCCCTACGGA ATCTCTGTTG 
AAGCGGCGCC CCTATGGCCG AAATAAGCCT CTGATCTCAC GCACTATGAT GAAGAACATC 
TTGGGCCATG CATTCTATCA GCTCATTGTC ATCTTTATCC TTGTCTTTGC GGGTGAGAAA 
TTCTTTGATA TTGATAGTGG GAGGAAGGCA CCTCTACATT CACCACCCAG CCAGCACTAT 
ACCATTGTTT TTAACACCTT CGTGCTGATG CAGCTCTTCA ATGAAATCAA CTCCCGAAAG 
ATCCATGGAG AGAAGAACGT CTTTTCAGGC ATCTACCGCA ACATTATCTT CTGCTCTGTA 
GTCTTGGGCA CATTCATCTG CCAGATTTTC ATCGTGGAAT TTGGGGGTAA ACCCTTCAGT 
TGTACAAGCC TCAGCCTGTC TCAGTGGCTG TGGTGTCTCT TCATTGGGAT TGGAGAACTT 
CTGTGGGGCC AGTTCATCTC CGCAATACCT ACCCGATCCC TGAAGTTCCT GAAGGAGGCT 
GGGCATGGCA CCACCAAAGA GGAGATCACC AAGGATGCCG AGGGACTGGA TGAGATTGAC 
CATGCTGAGA TGGAGCTGCG CCGAGGCCAG ATCCTCTGGT TCCGGGGCCT GAACCGTATC 
CAGACTCAGA TCAAAGTGGT CAAAGCGTTC CATAGTTCCC TCCACGAAAG CATTCAGAAA 
CCCTACAACC AAAAGTCCAT CCACAGCTTC ATGACCCACC CTGAATTCGC CATAGAGGAG 
GAGTTGCCAC GAACACCACT CCTGGATGAG GAAGAGGAGG AAAATCCTGA CAAGGCTTCT 
AAGTTTGGGA CTAGGGTGCT CCTGTTGGAT GGTGAGGTCA CTCCATATGC CAATACAAAC 
AACAATGCGG TGGATTGCAA CCAAGTGCAG CTCCCCCAGT CGGACAGCTC TCTACAGAGC 
CTAGAGACAT CAGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001675.3
CDS	898..4515
Translation	MTNPSDRVLPANSMAESREGDFGCTVMELRKLMELRSRDALTQINVHYGGVQNLCSRLKTSPVEGLSGNPADLEKRRQVFGHNVIPPKKPKTFLELVWEALQDVTLIILEIAAIISLVLSFYRPAGEENELCGQVATTPEDENEAQAGWIEGAAILFSVIIVVLVTAFNDWSKEKQFRGLQCRIEQEQKFSIIRNGQLIQLPVAEIVVGDIAQVKYGDLLPADGILIQGNDLKIDESSLTGESDHVKKSLDKDPMLLSGTHVMEGSGRMVVTAVGVNSQTGIILTLLGVNEDDEGEKKKKGKKQGVPENRNKAKTQDGVALEIQPLNSQEGIDNEEKDKKAVKVPKKEKSVLQGKLTRLAVQIGKAGLLMSALTVFILILYFVIDNFVINRRPWLPECTPIYIQYFVKFFIIGITVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTMNRMTVVQAYIGGIHYRQIPSPDVFLPKVLDLIVNGISINSAYTSKILPPEKEGGLPRQVGNKTECALLGFVTDLKQDYQAVRNEVPEEKLYKVYTFNSVRKSMSTVIRNPNGGFRMYSKGASEIILRKCNRILDRKGEAVPFKNKDRDDMVRTVIEPMACDGLRTICIAYRDFDDTEPSWDNENEILTELTCIAVVGIEDPVRPEVPDAIAKCKQAGITVRMVTGDNINTARAIATKCGILTPGDDFLCLEGKEFNRLIRNEKGEVEQEKLDKIWPKLRVLARSSPTDKHTLVKGIIDSTVGEHRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFTSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVIVAFTGACITQDSPLKAVQMLWVNLIMDTFASLALATEPPTESLLKRRPYGRNKPLISRTMMKNILGHAFYQLIVIFILVFAGEKFFDIDSGRKAPLHSPPSQHYTIVFNTFVLMQLFNEINSRKIHGEKNVFSGIYRNIIFCSVVLGTFICQIFIVEFGGKPFSCTSLSLSQWLWCLFIGIGELLWGQFISAIPTRSLKFLKEAGHGTTKEEITKDAEGLDEIDHAEMELRRGQILWFRGLNRIQTQIKVVKAFHSSLHESIQKPYNQKSIHSFMTHPEFAIEEELPRTPLLDEEEEENPDKASKFGTRVLLLDGEVTPYANTNNNAVDCNQVQLPQSDSSLQSLETSV

Target ORF information:

RefSeq Version	NM_001684.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ATPase plasma membrane Ca2+ transporting 4 (ATP2B4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001684.4

ORF Insert Sequence:

ATGACGAACC CATCAGACCG TGTCTTGCCT GCCAACTCGA TGGCCGAGAG CCGTGAAGGG 
GACTTTGGCT GCACAGTAAT GGAACTGAGG AAGCTCATGG AGCTGCGTTC AAGGGATGCA 
CTGACCCAGA TTAATGTCCA CTATGGAGGT GTACAGAATC TCTGCAGTAG ACTGAAAACC 
TCCCCTGTGG AAGGTCTGTC TGGGAACCCT GCAGATCTGG AGAAACGTAG GCAGGTGTTT 
GGACACAACG TGATCCCCCC CAAAAAGCCC AAGACTTTCT TAGAATTAGT GTGGGAAGCT 
CTTCAAGATG TCACGCTTAT CATCCTGGAG ATTGCAGCCA TCATCTCCCT GGTCCTGTCC 
TTTTATCGCC CTGCTGGTGA AGAAAATGAA CTGTGTGGTC AAGTCGCAAC TACCCCAGAA 
GATGAAAATG AGGCACAAGC TGGCTGGATT GAGGGGGCAG CCATCCTTTT CTCAGTGATC 
ATCGTGGTGT TAGTGACTGC CTTTAATGAT TGGAGCAAAG AGAAGCAATT CCGGGGGCTG 
CAGTGCCGCA TTGAACAGGA GCAAAAGTTC TCCATCATCC GAAACGGTCA ACTCATCCAG 
CTCCCTGTGG CTGAGATTGT GGTTGGTGAT ATTGCCCAAG TCAAATACGG TGATCTGCTG 
CCTGCAGATG GAATCCTGAT CCAAGGGAAT GATCTGAAGA TTGATGAGAG CTCTCTGACA 
GGGGAATCTG ACCATGTCAA GAAGTCCCTG GACAAAGACC CCATGTTGCT CTCAGGGACC 
CATGTCATGG AAGGTTCTGG CCGGATGGTG GTGACAGCTG TTGGTGTCAA CTCTCAGACT 
GGAATCATCC TTACTCTCTT GGGGGTCAAT GAGGATGACG AAGGGGAGAA AAAGAAGAAA 
GGTAAAAAAC AAGGAGTCCC TGAAAATCGC AACAAAGCAA AGACCCAAGA CGGAGTGGCC 
CTGGAAATCC AGCCACTCAA CAGCCAGGAG GGAATCGACA ATGAGGAAAA GGACAAGAAG 
GCAGTCAAGG TGCCTAAAAA GGAGAAGTCA GTGCTGCAGG GCAAGCTGAC TCGCCTGGCT 
GTTCAGATTG GGAAAGCCGG TCTGCTCATG TCTGCTCTCA CGGTTTTCAT CCTGATTCTA 
TACTTTGTGA TTGACAACTT TGTGATAAAT CGCAGACCAT GGCTCCCTGA GTGTACTCCC 
ATCTACATCC AGTACTTTGT CAAGTTCTTC ATCATCGGCA TCACTGTACT GGTGGTGGCT 
GTGCCAGAGG GGCTGCCTCT GGCTGTCACC ATCTCACTGG CCTACTCTGT GAAGAAAATG 
ATGAAAGACA ATAACCTAGT ACGGCACTTG GATGCTTGTG AGACCATGGG CAACGCCACC 
GCCATCTGCT CTGATAAGAC AGGCACGTTG ACCATGAACC GCATGACTGT GGTACAAGCT 
TATATTGGGG GCATCCATTA CCGTCAAATC CCAAGCCCTG ATGTCTTCCT GCCCAAAGTC 
CTGGACCTCA TTGTCAATGG CATTTCTATC AACAGTGCTT ATACCTCCAA GATTCTGCCT 
CCAGAGAAGG AGGGAGGCCT GCCTCGGCAG GTGGGCAACA AGACCGAGTG TGCTCTGCTA 
GGCTTTGTCA CAGATCTGAA GCAGGATTAT CAGGCTGTGC GTAATGAAGT GCCCGAGGAG 
AAGCTCTACA AGGTGTACAC CTTTAACTCA GTGCGCAAGT CAATGAGCAC CGTCATCAGG 
AATCCCAACG GTGGCTTCCG TATGTACAGC AAGGGCGCCT CTGAGATCAT CTTGCGCAAG 
TGTAATCGAA TCCTGGACCG GAAAGGGGAA GCAGTGCCAT TCAAGAATAA AGACAGAGAT 
GATATGGTAC GCACTGTCAT CGAGCCCATG GCCTGTGATG GACTCCGGAC TATCTGCATA 
GCTTACCGGG ACTTCGATGA CACAGAGCCC TCTTGGGACA ATGAGAATGA GATCCTCACC 
GAACTGACCT GTATCGCGGT GGTGGGCATT GAGGACCCTG TGCGCCCAGA GGTGCCAGAT 
GCTATTGCCA AATGCAAACA AGCTGGCATT ACTGTCAGAA TGGTGACAGG TGACAACATC 
AACACAGCCC GGGCCATTGC CACCAAATGT GGCATTCTGA CACCTGGGGA TGACTTCCTG 
TGCTTAGAAG GCAAAGAATT CAACCGGCTC ATCCGCAACG AGAAAGGCGA GGTAGAGCAA 
GAAAAGCTGG ACAAGATCTG GCCTAAGCTT CGGGTCCTGG CGCGATCTTC TCCCACTGAC 
AAGCACACCC TGGTGAAAGG CATAATTGAC AGCACTGTTG GGGAACACCG GCAGGTCGTG 
GCTGTCACTG GTGATGGCAC AAATGACGGG CCTGCTCTGA AGAAAGCGGA TGTTGGTTTT 
GCCATGGGCA TCGCAGGCAC AGATGTAGCA AAGGAGGCTT CAGACATCAT CCTAACAGAT 
GACAACTTCA CCAGCATTGT GAAGGCAGTG ATGTGGGGAC GAAATGTCTA TGACAGCATC 
TCCAAGTTCC TGCAGTTCCA GCTCACTGTC AATGTGGTGG CCGTGATTGT AGCCTTCACT 
GGAGCCTGTA TCACTCAGGA TTCCCCATTG AAAGCTGTGC AGATGTTGTG GGTTAATCTG 
ATCATGGACA CTTTTGCTTC ATTGGCCCTG GCCACAGAGC CCCCTACGGA ATCTCTGTTG 
AAGCGGCGCC CCTATGGCCG AAATAAGCCT CTGATCTCAC GCACTATGAT GAAGAACATC 
TTGGGCCATG CATTCTATCA GCTCATTGTC ATCTTTATCC TTGTCTTTGC GGGTGAGAAA 
TTCTTTGATA TTGATAGTGG GAGGAAGGCA CCTCTACATT CACCACCCAG CCAGCACTAT 
ACCATTGTTT TTAACACCTT CGTGCTGATG CAGCTCTTCA ATGAAATCAA CTCCCGAAAG 
ATCCATGGAG AGAAGAACGT CTTTTCAGGC ATCTACCGCA ACATTATCTT CTGCTCTGTA 
GTCTTGGGCA CATTCATCTG CCAGATTTTC ATCGTGGAAT TTGGGGGTAA ACCCTTCAGT 
TGTACAAGCC TCAGCCTGTC TCAGTGGCTG TGGTGTCTCT TCATTGGGAT TGGAGAACTT 
CTGTGGGGCC AGTTCATCTC CGCAATACCT ACCCGATCCC TGAAGTTCCT GAAGGAGGCT 
GGGCATGGCA CCACCAAAGA GGAGATCACC AAGGATGCCG AGGGACTGGA TGAGATTGAC 
CATGCTGAGA TGGAGCTGCG CCGAGGCCAG ATCCTCTGGT TCCGGGGCCT GAACCGTATC 
CAGACTCAGA TCAAAGTGGT CAAAGCGTTC CATAGTTCCC TCCACGAAAG CATTCAGAAA 
CCCTACAACC AAAAGTCCAT CCACAGCTTC ATGACCCACC CTGAATTCGC CATAGAGGAG 
GAGTTGCCAC GAACACCACT CCTGGATGAG GAAGAGGAGG AAAATCCTGA CAAGGCTTCT 
AAGTTTGGGA CTAGGGTGCT CCTGTTGGAT GGTGAGGTCA CTCCATATGC CAATACAAAC 
AACAATGCGG TGGATTGCAA CCAAGTGCAG CTCCCCCAGT CGGACAGCTC TCTACAGAGC 
CTAGAGACAT CAGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATP2B4 ( NM_001684.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_001675.3 Homo sapiens ATPase plasma membrane Ca2+ transporting 4 (ATP2B4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu14814
Clone ID Related Accession (Same CDS sequence)	NM_001684.4 , NM_001684.5
Accession Version	NM_001684.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-29
Organism	Homo sapiens(human)
Product	plasma membrane calcium-transporting ATPase 4 isoform 4b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL706212.1, DN997129.1, M25874.1, BF985985.1, AC114402.2 and AW888226.1. On Jun 2, 2019 this sequence version replaced NM_001684.4. Transcript Variant: This variant (2) lacks an alternate coding exon compared to variant 1, that causes a frameshift. The resulting isoform (4b) has a longer and distinct C-terminus compared to isoform 4a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX537444.1, CR749393.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGACGAACC CATCAGACCG TGTCTTGCCT GCCAACTCGA TGGCCGAGAG CCGTGAAGGG 
GACTTTGGCT GCACAGTAAT GGAACTGAGG AAGCTCATGG AGCTGCGTTC AAGGGATGCA 
CTGACCCAGA TTAATGTCCA CTATGGAGGT GTACAGAATC TCTGCAGTAG ACTGAAAACC 
TCCCCTGTGG AAGGTCTGTC TGGGAACCCT GCAGATCTGG AGAAACGTAG GCAGGTGTTT 
GGACACAACG TGATCCCCCC CAAAAAGCCC AAGACTTTCT TAGAATTAGT GTGGGAAGCT 
CTTCAAGATG TCACGCTTAT CATCCTGGAG ATTGCAGCCA TCATCTCCCT GGTCCTGTCC 
TTTTATCGCC CTGCTGGTGA AGAAAATGAA CTGTGTGGTC AAGTCGCAAC TACCCCAGAA 
GATGAAAATG AGGCACAAGC TGGCTGGATT GAGGGGGCAG CCATCCTTTT CTCAGTGATC 
ATCGTGGTGT TAGTGACTGC CTTTAATGAT TGGAGCAAAG AGAAGCAATT CCGGGGGCTG 
CAGTGCCGCA TTGAACAGGA GCAAAAGTTC TCCATCATCC GAAACGGTCA ACTCATCCAG 
CTCCCTGTGG CTGAGATTGT GGTTGGTGAT ATTGCCCAAG TCAAATACGG TGATCTGCTG 
CCTGCAGATG GAATCCTGAT CCAAGGGAAT GATCTGAAGA TTGATGAGAG CTCTCTGACA 
GGGGAATCTG ACCATGTCAA GAAGTCCCTG GACAAAGACC CCATGTTGCT CTCAGGGACC 
CATGTCATGG AAGGTTCTGG CCGGATGGTG GTGACAGCTG TTGGTGTCAA CTCTCAGACT 
GGAATCATCC TTACTCTCTT GGGGGTCAAT GAGGATGACG AAGGGGAGAA AAAGAAGAAA 
GGTAAAAAAC AAGGAGTCCC TGAAAATCGC AACAAAGCAA AGACCCAAGA CGGAGTGGCC 
CTGGAAATCC AGCCACTCAA CAGCCAGGAG GGAATCGACA ATGAGGAAAA GGACAAGAAG 
GCAGTCAAGG TGCCTAAAAA GGAGAAGTCA GTGCTGCAGG GCAAGCTGAC TCGCCTGGCT 
GTTCAGATTG GGAAAGCCGG TCTGCTCATG TCTGCTCTCA CGGTTTTCAT CCTGATTCTA 
TACTTTGTGA TTGACAACTT TGTGATAAAT CGCAGACCAT GGCTCCCTGA GTGTACTCCC 
ATCTACATCC AGTACTTTGT CAAGTTCTTC ATCATCGGCA TCACTGTACT GGTGGTGGCT 
GTGCCAGAGG GGCTGCCTCT GGCTGTCACC ATCTCACTGG CCTACTCTGT GAAGAAAATG 
ATGAAAGACA ATAACCTAGT ACGGCACTTG GATGCTTGTG AGACCATGGG CAACGCCACC 
GCCATCTGCT CTGATAAGAC AGGCACGTTG ACCATGAACC GCATGACTGT GGTACAAGCT 
TATATTGGGG GCATCCATTA CCGTCAAATC CCAAGCCCTG ATGTCTTCCT GCCCAAAGTC 
CTGGACCTCA TTGTCAATGG CATTTCTATC AACAGTGCTT ATACCTCCAA GATTCTGCCT 
CCAGAGAAGG AGGGAGGCCT GCCTCGGCAG GTGGGCAACA AGACCGAGTG TGCTCTGCTA 
GGCTTTGTCA CAGATCTGAA GCAGGATTAT CAGGCTGTGC GTAATGAAGT GCCCGAGGAG 
AAGCTCTACA AGGTGTACAC CTTTAACTCA GTGCGCAAGT CAATGAGCAC CGTCATCAGG 
AATCCCAACG GTGGCTTCCG TATGTACAGC AAGGGCGCCT CTGAGATCAT CTTGCGCAAG 
TGTAATCGAA TCCTGGACCG GAAAGGGGAA GCAGTGCCAT TCAAGAATAA AGACAGAGAT 
GATATGGTAC GCACTGTCAT CGAGCCCATG GCCTGTGATG GACTCCGGAC TATCTGCATA 
GCTTACCGGG ACTTCGATGA CACAGAGCCC TCTTGGGACA ATGAGAATGA GATCCTCACC 
GAACTGACCT GTATCGCGGT GGTGGGCATT GAGGACCCTG TGCGCCCAGA GGTGCCAGAT 
GCTATTGCCA AATGCAAACA AGCTGGCATT ACTGTCAGAA TGGTGACAGG TGACAACATC 
AACACAGCCC GGGCCATTGC CACCAAATGT GGCATTCTGA CACCTGGGGA TGACTTCCTG 
TGCTTAGAAG GCAAAGAATT CAACCGGCTC ATCCGCAACG AGAAAGGCGA GGTAGAGCAA 
GAAAAGCTGG ACAAGATCTG GCCTAAGCTT CGGGTCCTGG CGCGATCTTC TCCCACTGAC 
AAGCACACCC TGGTGAAAGG CATAATTGAC AGCACTGTTG GGGAACACCG GCAGGTCGTG 
GCTGTCACTG GTGATGGCAC AAATGACGGG CCTGCTCTGA AGAAAGCGGA TGTTGGTTTT 
GCCATGGGCA TCGCAGGCAC AGATGTAGCA AAGGAGGCTT CAGACATCAT CCTAACAGAT 
GACAACTTCA CCAGCATTGT GAAGGCAGTG ATGTGGGGAC GAAATGTCTA TGACAGCATC 
TCCAAGTTCC TGCAGTTCCA GCTCACTGTC AATGTGGTGG CCGTGATTGT AGCCTTCACT 
GGAGCCTGTA TCACTCAGGA TTCCCCATTG AAAGCTGTGC AGATGTTGTG GGTTAATCTG 
ATCATGGACA CTTTTGCTTC ATTGGCCCTG GCCACAGAGC CCCCTACGGA ATCTCTGTTG 
AAGCGGCGCC CCTATGGCCG AAATAAGCCT CTGATCTCAC GCACTATGAT GAAGAACATC 
TTGGGCCATG CATTCTATCA GCTCATTGTC ATCTTTATCC TTGTCTTTGC GGGTGAGAAA 
TTCTTTGATA TTGATAGTGG GAGGAAGGCA CCTCTACATT CACCACCCAG CCAGCACTAT 
ACCATTGTTT TTAACACCTT CGTGCTGATG CAGCTCTTCA ATGAAATCAA CTCCCGAAAG 
ATCCATGGAG AGAAGAACGT CTTTTCAGGC ATCTACCGCA ACATTATCTT CTGCTCTGTA 
GTCTTGGGCA CATTCATCTG CCAGATTTTC ATCGTGGAAT TTGGGGGTAA ACCCTTCAGT 
TGTACAAGCC TCAGCCTGTC TCAGTGGCTG TGGTGTCTCT TCATTGGGAT TGGAGAACTT 
CTGTGGGGCC AGTTCATCTC CGCAATACCT ACCCGATCCC TGAAGTTCCT GAAGGAGGCT 
GGGCATGGCA CCACCAAAGA GGAGATCACC AAGGATGCCG AGGGACTGGA TGAGATTGAC 
CATGCTGAGA TGGAGCTGCG CCGAGGCCAG ATCCTCTGGT TCCGGGGCCT GAACCGTATC 
CAGACTCAGA TCAAAGTGGT CAAAGCGTTC CATAGTTCCC TCCACGAAAG CATTCAGAAA 
CCCTACAACC AAAAGTCCAT CCACAGCTTC ATGACCCACC CTGAATTCGC CATAGAGGAG 
GAGTTGCCAC GAACACCACT CCTGGATGAG GAAGAGGAGG AAAATCCTGA CAAGGCTTCT 
AAGTTTGGGA CTAGGGTGCT CCTGTTGGAT GGTGAGGTCA CTCCATATGC CAATACAAAC 
AACAATGCGG TGGATTGCAA CCAAGTGCAG CTCCCCCAGT CGGACAGCTC TCTACAGAGC 
CTAGAGACAT CAGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001675.3
CDS	853..4470
Translation	MTNPSDRVLPANSMAESREGDFGCTVMELRKLMELRSRDALTQINVHYGGVQNLCSRLKTSPVEGLSGNPADLEKRRQVFGHNVIPPKKPKTFLELVWEALQDVTLIILEIAAIISLVLSFYRPAGEENELCGQVATTPEDENEAQAGWIEGAAILFSVIIVVLVTAFNDWSKEKQFRGLQCRIEQEQKFSIIRNGQLIQLPVAEIVVGDIAQVKYGDLLPADGILIQGNDLKIDESSLTGESDHVKKSLDKDPMLLSGTHVMEGSGRMVVTAVGVNSQTGIILTLLGVNEDDEGEKKKKGKKQGVPENRNKAKTQDGVALEIQPLNSQEGIDNEEKDKKAVKVPKKEKSVLQGKLTRLAVQIGKAGLLMSALTVFILILYFVIDNFVINRRPWLPECTPIYIQYFVKFFIIGITVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTMNRMTVVQAYIGGIHYRQIPSPDVFLPKVLDLIVNGISINSAYTSKILPPEKEGGLPRQVGNKTECALLGFVTDLKQDYQAVRNEVPEEKLYKVYTFNSVRKSMSTVIRNPNGGFRMYSKGASEIILRKCNRILDRKGEAVPFKNKDRDDMVRTVIEPMACDGLRTICIAYRDFDDTEPSWDNENEILTELTCIAVVGIEDPVRPEVPDAIAKCKQAGITVRMVTGDNINTARAIATKCGILTPGDDFLCLEGKEFNRLIRNEKGEVEQEKLDKIWPKLRVLARSSPTDKHTLVKGIIDSTVGEHRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFTSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVIVAFTGACITQDSPLKAVQMLWVNLIMDTFASLALATEPPTESLLKRRPYGRNKPLISRTMMKNILGHAFYQLIVIFILVFAGEKFFDIDSGRKAPLHSPPSQHYTIVFNTFVLMQLFNEINSRKIHGEKNVFSGIYRNIIFCSVVLGTFICQIFIVEFGGKPFSCTSLSLSQWLWCLFIGIGELLWGQFISAIPTRSLKFLKEAGHGTTKEEITKDAEGLDEIDHAEMELRRGQILWFRGLNRIQTQIKVVKAFHSSLHESIQKPYNQKSIHSFMTHPEFAIEEELPRTPLLDEEEEENPDKASKFGTRVLLLDGEVTPYANTNNNAVDCNQVQLPQSDSSLQSLETSV

Target ORF information:

RefSeq Version	NM_001684.5
Organism	Homo sapiens(human)
Definition	Homo sapiens ATPase plasma membrane Ca2+ transporting 4 (ATP2B4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001684.5

ORF Insert Sequence:

ATGACGAACC CATCAGACCG TGTCTTGCCT GCCAACTCGA TGGCCGAGAG CCGTGAAGGG 
GACTTTGGCT GCACAGTAAT GGAACTGAGG AAGCTCATGG AGCTGCGTTC AAGGGATGCA 
CTGACCCAGA TTAATGTCCA CTATGGAGGT GTACAGAATC TCTGCAGTAG ACTGAAAACC 
TCCCCTGTGG AAGGTCTGTC TGGGAACCCT GCAGATCTGG AGAAACGTAG GCAGGTGTTT 
GGACACAACG TGATCCCCCC CAAAAAGCCC AAGACTTTCT TAGAATTAGT GTGGGAAGCT 
CTTCAAGATG TCACGCTTAT CATCCTGGAG ATTGCAGCCA TCATCTCCCT GGTCCTGTCC 
TTTTATCGCC CTGCTGGTGA AGAAAATGAA CTGTGTGGTC AAGTCGCAAC TACCCCAGAA 
GATGAAAATG AGGCACAAGC TGGCTGGATT GAGGGGGCAG CCATCCTTTT CTCAGTGATC 
ATCGTGGTGT TAGTGACTGC CTTTAATGAT TGGAGCAAAG AGAAGCAATT CCGGGGGCTG 
CAGTGCCGCA TTGAACAGGA GCAAAAGTTC TCCATCATCC GAAACGGTCA ACTCATCCAG 
CTCCCTGTGG CTGAGATTGT GGTTGGTGAT ATTGCCCAAG TCAAATACGG TGATCTGCTG 
CCTGCAGATG GAATCCTGAT CCAAGGGAAT GATCTGAAGA TTGATGAGAG CTCTCTGACA 
GGGGAATCTG ACCATGTCAA GAAGTCCCTG GACAAAGACC CCATGTTGCT CTCAGGGACC 
CATGTCATGG AAGGTTCTGG CCGGATGGTG GTGACAGCTG TTGGTGTCAA CTCTCAGACT 
GGAATCATCC TTACTCTCTT GGGGGTCAAT GAGGATGACG AAGGGGAGAA AAAGAAGAAA 
GGTAAAAAAC AAGGAGTCCC TGAAAATCGC AACAAAGCAA AGACCCAAGA CGGAGTGGCC 
CTGGAAATCC AGCCACTCAA CAGCCAGGAG GGAATCGACA ATGAGGAAAA GGACAAGAAG 
GCAGTCAAGG TGCCTAAAAA GGAGAAGTCA GTGCTGCAGG GCAAGCTGAC TCGCCTGGCT 
GTTCAGATTG GGAAAGCCGG TCTGCTCATG TCTGCTCTCA CGGTTTTCAT CCTGATTCTA 
TACTTTGTGA TTGACAACTT TGTGATAAAT CGCAGACCAT GGCTCCCTGA GTGTACTCCC 
ATCTACATCC AGTACTTTGT CAAGTTCTTC ATCATCGGCA TCACTGTACT GGTGGTGGCT 
GTGCCAGAGG GGCTGCCTCT GGCTGTCACC ATCTCACTGG CCTACTCTGT GAAGAAAATG 
ATGAAAGACA ATAACCTAGT ACGGCACTTG GATGCTTGTG AGACCATGGG CAACGCCACC 
GCCATCTGCT CTGATAAGAC AGGCACGTTG ACCATGAACC GCATGACTGT GGTACAAGCT 
TATATTGGGG GCATCCATTA CCGTCAAATC CCAAGCCCTG ATGTCTTCCT GCCCAAAGTC 
CTGGACCTCA TTGTCAATGG CATTTCTATC AACAGTGCTT ATACCTCCAA GATTCTGCCT 
CCAGAGAAGG AGGGAGGCCT GCCTCGGCAG GTGGGCAACA AGACCGAGTG TGCTCTGCTA 
GGCTTTGTCA CAGATCTGAA GCAGGATTAT CAGGCTGTGC GTAATGAAGT GCCCGAGGAG 
AAGCTCTACA AGGTGTACAC CTTTAACTCA GTGCGCAAGT CAATGAGCAC CGTCATCAGG 
AATCCCAACG GTGGCTTCCG TATGTACAGC AAGGGCGCCT CTGAGATCAT CTTGCGCAAG 
TGTAATCGAA TCCTGGACCG GAAAGGGGAA GCAGTGCCAT TCAAGAATAA AGACAGAGAT 
GATATGGTAC GCACTGTCAT CGAGCCCATG GCCTGTGATG GACTCCGGAC TATCTGCATA 
GCTTACCGGG ACTTCGATGA CACAGAGCCC TCTTGGGACA ATGAGAATGA GATCCTCACC 
GAACTGACCT GTATCGCGGT GGTGGGCATT GAGGACCCTG TGCGCCCAGA GGTGCCAGAT 
GCTATTGCCA AATGCAAACA AGCTGGCATT ACTGTCAGAA TGGTGACAGG TGACAACATC 
AACACAGCCC GGGCCATTGC CACCAAATGT GGCATTCTGA CACCTGGGGA TGACTTCCTG 
TGCTTAGAAG GCAAAGAATT CAACCGGCTC ATCCGCAACG AGAAAGGCGA GGTAGAGCAA 
GAAAAGCTGG ACAAGATCTG GCCTAAGCTT CGGGTCCTGG CGCGATCTTC TCCCACTGAC 
AAGCACACCC TGGTGAAAGG CATAATTGAC AGCACTGTTG GGGAACACCG GCAGGTCGTG 
GCTGTCACTG GTGATGGCAC AAATGACGGG CCTGCTCTGA AGAAAGCGGA TGTTGGTTTT 
GCCATGGGCA TCGCAGGCAC AGATGTAGCA AAGGAGGCTT CAGACATCAT CCTAACAGAT 
GACAACTTCA CCAGCATTGT GAAGGCAGTG ATGTGGGGAC GAAATGTCTA TGACAGCATC 
TCCAAGTTCC TGCAGTTCCA GCTCACTGTC AATGTGGTGG CCGTGATTGT AGCCTTCACT 
GGAGCCTGTA TCACTCAGGA TTCCCCATTG AAAGCTGTGC AGATGTTGTG GGTTAATCTG 
ATCATGGACA CTTTTGCTTC ATTGGCCCTG GCCACAGAGC CCCCTACGGA ATCTCTGTTG 
AAGCGGCGCC CCTATGGCCG AAATAAGCCT CTGATCTCAC GCACTATGAT GAAGAACATC 
TTGGGCCATG CATTCTATCA GCTCATTGTC ATCTTTATCC TTGTCTTTGC GGGTGAGAAA 
TTCTTTGATA TTGATAGTGG GAGGAAGGCA CCTCTACATT CACCACCCAG CCAGCACTAT 
ACCATTGTTT TTAACACCTT CGTGCTGATG CAGCTCTTCA ATGAAATCAA CTCCCGAAAG 
ATCCATGGAG AGAAGAACGT CTTTTCAGGC ATCTACCGCA ACATTATCTT CTGCTCTGTA 
GTCTTGGGCA CATTCATCTG CCAGATTTTC ATCGTGGAAT TTGGGGGTAA ACCCTTCAGT 
TGTACAAGCC TCAGCCTGTC TCAGTGGCTG TGGTGTCTCT TCATTGGGAT TGGAGAACTT 
CTGTGGGGCC AGTTCATCTC CGCAATACCT ACCCGATCCC TGAAGTTCCT GAAGGAGGCT 
GGGCATGGCA CCACCAAAGA GGAGATCACC AAGGATGCCG AGGGACTGGA TGAGATTGAC 
CATGCTGAGA TGGAGCTGCG CCGAGGCCAG ATCCTCTGGT TCCGGGGCCT GAACCGTATC 
CAGACTCAGA TCAAAGTGGT CAAAGCGTTC CATAGTTCCC TCCACGAAAG CATTCAGAAA 
CCCTACAACC AAAAGTCCAT CCACAGCTTC ATGACCCACC CTGAATTCGC CATAGAGGAG 
GAGTTGCCAC GAACACCACT CCTGGATGAG GAAGAGGAGG AAAATCCTGA CAAGGCTTCT 
AAGTTTGGGA CTAGGGTGCT CCTGTTGGAT GGTGAGGTCA CTCCATATGC CAATACAAAC 
AACAATGCGG TGGATTGCAA CCAAGTGCAG CTCCCCCAGT CGGACAGCTC TCTACAGAGC 
CTAGAGACAT CAGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATP2B4 ( NM_001001396.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001001396.1 Homo sapiens ATPase plasma membrane Ca2+ transporting 4 (ATP2B4), transcript variant 1, mRNA.

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CloneID	OHu25104
Clone ID Related Accession (Same CDS sequence)	NM_001001396.2
Accession Version	NM_001001396.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Homo sapiens(human)
Product	plasma membrane calcium-transporting ATPase 4 isoform 4a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX537444.1, AL706212.1, DN997129.1, M83363.1, BF985985.1, AC114402.2 and AW888226.1. This sequence is a reference standard in the RefSeqGene project. On Jul 19, 2011 this sequence version replaced NM_001001396.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the shorter isoform (4a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.31275.1, SRR1660807.77355.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACGAACC CATCAGACCG TGTCTTGCCT GCCAACTCGA TGGCCGAGAG CCGTGAAGGG 
GACTTTGGCT GCACAGTAAT GGAACTGAGG AAGCTCATGG AGCTGCGTTC AAGGGATGCA 
CTGACCCAGA TTAATGTCCA CTATGGAGGT GTACAGAATC TCTGCAGTAG ACTGAAAACC 
TCCCCTGTGG AAGGTCTGTC TGGGAACCCT GCAGATCTGG AGAAACGTAG GCAGGTGTTT 
GGACACAACG TGATCCCCCC CAAAAAGCCC AAGACTTTCT TAGAATTAGT GTGGGAAGCT 
CTTCAAGATG TCACGCTTAT CATCCTGGAG ATTGCAGCCA TCATCTCCCT GGTCCTGTCC 
TTTTATCGCC CTGCTGGTGA AGAAAATGAA CTGTGTGGTC AAGTCGCAAC TACCCCAGAA 
GATGAAAATG AGGCACAAGC TGGCTGGATT GAGGGGGCAG CCATCCTTTT CTCAGTGATC 
ATCGTGGTGT TAGTGACTGC CTTTAATGAT TGGAGCAAAG AGAAGCAATT CCGGGGGCTG 
CAGTGCCGCA TTGAACAGGA GCAAAAGTTC TCCATCATCC GAAACGGTCA ACTCATCCAG 
CTCCCTGTGG CTGAGATTGT GGTTGGTGAT ATTGCCCAAG TCAAATACGG TGATCTGCTG 
CCTGCAGATG GAATCCTGAT CCAAGGGAAT GATCTGAAGA TTGATGAGAG CTCTCTGACA 
GGGGAATCTG ACCATGTCAA GAAGTCCCTG GACAAAGACC CCATGTTGCT CTCAGGGACC 
CATGTCATGG AAGGTTCTGG CCGGATGGTG GTGACAGCTG TTGGTGTCAA CTCTCAGACT 
GGAATCATCC TTACTCTCTT GGGGGTCAAT GAGGATGACG AAGGGGAGAA AAAGAAGAAA 
GGTAAAAAAC AAGGAGTCCC TGAAAATCGC AACAAAGCAA AGACCCAAGA CGGAGTGGCC 
CTGGAAATCC AGCCACTCAA CAGCCAGGAG GGAATCGACA ATGAGGAAAA GGACAAGAAG 
GCAGTCAAGG TGCCTAAAAA GGAGAAGTCA GTGCTGCAGG GCAAGCTGAC TCGCCTGGCT 
GTTCAGATTG GGAAAGCCGG TCTGCTCATG TCTGCTCTCA CGGTTTTCAT CCTGATTCTA 
TACTTTGTGA TTGACAACTT TGTGATAAAT CGCAGACCAT GGCTCCCTGA GTGTACTCCC 
ATCTACATCC AGTACTTTGT CAAGTTCTTC ATCATCGGCA TCACTGTACT GGTGGTGGCT 
GTGCCAGAGG GGCTGCCTCT GGCTGTCACC ATCTCACTGG CCTACTCTGT GAAGAAAATG 
ATGAAAGACA ATAACCTAGT ACGGCACTTG GATGCTTGTG AGACCATGGG CAACGCCACC 
GCCATCTGCT CTGATAAGAC AGGCACGTTG ACCATGAACC GCATGACTGT GGTACAAGCT 
TATATTGGGG GCATCCATTA CCGTCAAATC CCAAGCCCTG ATGTCTTCCT GCCCAAAGTC 
CTGGACCTCA TTGTCAATGG CATTTCTATC AACAGTGCTT ATACCTCCAA GATTCTGCCT 
CCAGAGAAGG AGGGAGGCCT GCCTCGGCAG GTGGGCAACA AGACCGAGTG TGCTCTGCTA 
GGCTTTGTCA CAGATCTGAA GCAGGATTAT CAGGCTGTGC GTAATGAAGT GCCCGAGGAG 
AAGCTCTACA AGGTGTACAC CTTTAACTCA GTGCGCAAGT CAATGAGCAC CGTCATCAGG 
AATCCCAACG GTGGCTTCCG TATGTACAGC AAGGGCGCCT CTGAGATCAT CTTGCGCAAG 
TGTAATCGAA TCCTGGACCG GAAAGGGGAA GCAGTGCCAT TCAAGAATAA AGACAGAGAT 
GATATGGTAC GCACTGTCAT CGAGCCCATG GCCTGTGATG GACTCCGGAC TATCTGCATA 
GCTTACCGGG ACTTCGATGA CACAGAGCCC TCTTGGGACA ATGAGAATGA GATCCTCACC 
GAACTGACCT GTATCGCGGT GGTGGGCATT GAGGACCCTG TGCGCCCAGA GGTGCCAGAT 
GCTATTGCCA AATGCAAACA AGCTGGCATT ACTGTCAGAA TGGTGACAGG TGACAACATC 
AACACAGCCC GGGCCATTGC CACCAAATGT GGCATTCTGA CACCTGGGGA TGACTTCCTG 
TGCTTAGAAG GCAAAGAATT CAACCGGCTC ATCCGCAACG AGAAAGGCGA GGTAGAGCAA 
GAAAAGCTGG ACAAGATCTG GCCTAAGCTT CGGGTCCTGG CGCGATCTTC TCCCACTGAC 
AAGCACACCC TGGTGAAAGG CATAATTGAC AGCACTGTTG GGGAACACCG GCAGGTCGTG 
GCTGTCACTG GTGATGGCAC AAATGACGGG CCTGCTCTGA AGAAAGCGGA TGTTGGTTTT 
GCCATGGGCA TCGCAGGCAC AGATGTAGCA AAGGAGGCTT CAGACATCAT CCTAACAGAT 
GACAACTTCA CCAGCATTGT GAAGGCAGTG ATGTGGGGAC GAAATGTCTA TGACAGCATC 
TCCAAGTTCC TGCAGTTCCA GCTCACTGTC AATGTGGTGG CCGTGATTGT AGCCTTCACT 
GGAGCCTGTA TCACTCAGGA TTCCCCATTG AAAGCTGTGC AGATGTTGTG GGTTAATCTG 
ATCATGGACA CTTTTGCTTC ATTGGCCCTG GCCACAGAGC CCCCTACGGA ATCTCTGTTG 
AAGCGGCGCC CCTATGGCCG AAATAAGCCT CTGATCTCAC GCACTATGAT GAAGAACATC 
TTGGGCCATG CATTCTATCA GCTCATTGTC ATCTTTATCC TTGTCTTTGC GGGTGAGAAA 
TTCTTTGATA TTGATAGTGG GAGGAAGGCA CCTCTACATT CACCACCCAG CCAGCACTAT 
ACCATTGTTT TTAACACCTT CGTGCTGATG CAGCTCTTCA ATGAAATCAA CTCCCGAAAG 
ATCCATGGAG AGAAGAACGT CTTTTCAGGC ATCTACCGCA ACATTATCTT CTGCTCTGTA 
GTCTTGGGCA CATTCATCTG CCAGATTTTC ATCGTGGAAT TTGGGGGTAA ACCCTTCAGT 
TGTACAAGCC TCAGCCTGTC TCAGTGGCTG TGGTGTCTCT TCATTGGGAT TGGAGAACTT 
CTGTGGGGCC AGTTCATCTC CGCAATACCT ACCCGATCCC TGAAGTTCCT GAAGGAGGCT 
GGGCATGGCA CCACCAAAGA GGAGATCACC AAGGATGCCG AGGGACTGGA TGAGATTGAC 
CATGCTGAGA TGGAGCTGCG CCGAGGCCAG ATCCTCTGGT TCCGGGGCCT GAACCGTATC 
CAGACTCAGA TCGACGTAAT TAACACATTC CAGACGGGAG CCTCTTTTAA GGGAGTCCTA 
AGGCGACAGA ACATGGGTCA ACACCTTGAT GTAAAACTTG TTCCTAGTTC ATCCTATGTA 
GCAGTTGCAC CAGTCAAATC TTCCCCCACC ACTTCTGTTC CTGCTGTTTC ATCTCCTCCT 
ATGGGCAATC AAAGTGGTCA AAGCGTTCCA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001001396.1
CDS	898..4410
Translation	MTNPSDRVLPANSMAESREGDFGCTVMELRKLMELRSRDALTQINVHYGGVQNLCSRLKTSPVEGLSGNPADLEKRRQVFGHNVIPPKKPKTFLELVWEALQDVTLIILEIAAIISLVLSFYRPAGEENELCGQVATTPEDENEAQAGWIEGAAILFSVIIVVLVTAFNDWSKEKQFRGLQCRIEQEQKFSIIRNGQLIQLPVAEIVVGDIAQVKYGDLLPADGILIQGNDLKIDESSLTGESDHVKKSLDKDPMLLSGTHVMEGSGRMVVTAVGVNSQTGIILTLLGVNEDDEGEKKKKGKKQGVPENRNKAKTQDGVALEIQPLNSQEGIDNEEKDKKAVKVPKKEKSVLQGKLTRLAVQIGKAGLLMSALTVFILILYFVIDNFVINRRPWLPECTPIYIQYFVKFFIIGITVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTMNRMTVVQAYIGGIHYRQIPSPDVFLPKVLDLIVNGISINSAYTSKILPPEKEGGLPRQVGNKTECALLGFVTDLKQDYQAVRNEVPEEKLYKVYTFNSVRKSMSTVIRNPNGGFRMYSKGASEIILRKCNRILDRKGEAVPFKNKDRDDMVRTVIEPMACDGLRTICIAYRDFDDTEPSWDNENEILTELTCIAVVGIEDPVRPEVPDAIAKCKQAGITVRMVTGDNINTARAIATKCGILTPGDDFLCLEGKEFNRLIRNEKGEVEQEKLDKIWPKLRVLARSSPTDKHTLVKGIIDSTVGEHRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFTSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVIVAFTGACITQDSPLKAVQMLWVNLIMDTFASLALATEPPTESLLKRRPYGRNKPLISRTMMKNILGHAFYQLIVIFILVFAGEKFFDIDSGRKAPLHSPPSQHYTIVFNTFVLMQLFNEINSRKIHGEKNVFSGIYRNIIFCSVVLGTFICQIFIVEFGGKPFSCTSLSLSQWLWCLFIGIGELLWGQFISAIPTRSLKFLKEAGHGTTKEEITKDAEGLDEIDHAEMELRRGQILWFRGLNRIQTQIDVINTFQTGASFKGVLRRQNMGQHLDVKLVPSSSYVAVAPVKSSPTTSVPAVSSPPMGNQSGQSVP

Target ORF information:

RefSeq Version	NM_001001396.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ATPase plasma membrane Ca2+ transporting 4 (ATP2B4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001001396.2

ORF Insert Sequence:

ATGACGAACC CATCAGACCG TGTCTTGCCT GCCAACTCGA TGGCCGAGAG CCGTGAAGGG 
GACTTTGGCT GCACAGTAAT GGAACTGAGG AAGCTCATGG AGCTGCGTTC AAGGGATGCA 
CTGACCCAGA TTAATGTCCA CTATGGAGGT GTACAGAATC TCTGCAGTAG ACTGAAAACC 
TCCCCTGTGG AAGGTCTGTC TGGGAACCCT GCAGATCTGG AGAAACGTAG GCAGGTGTTT 
GGACACAACG TGATCCCCCC CAAAAAGCCC AAGACTTTCT TAGAATTAGT GTGGGAAGCT 
CTTCAAGATG TCACGCTTAT CATCCTGGAG ATTGCAGCCA TCATCTCCCT GGTCCTGTCC 
TTTTATCGCC CTGCTGGTGA AGAAAATGAA CTGTGTGGTC AAGTCGCAAC TACCCCAGAA 
GATGAAAATG AGGCACAAGC TGGCTGGATT GAGGGGGCAG CCATCCTTTT CTCAGTGATC 
ATCGTGGTGT TAGTGACTGC CTTTAATGAT TGGAGCAAAG AGAAGCAATT CCGGGGGCTG 
CAGTGCCGCA TTGAACAGGA GCAAAAGTTC TCCATCATCC GAAACGGTCA ACTCATCCAG 
CTCCCTGTGG CTGAGATTGT GGTTGGTGAT ATTGCCCAAG TCAAATACGG TGATCTGCTG 
CCTGCAGATG GAATCCTGAT CCAAGGGAAT GATCTGAAGA TTGATGAGAG CTCTCTGACA 
GGGGAATCTG ACCATGTCAA GAAGTCCCTG GACAAAGACC CCATGTTGCT CTCAGGGACC 
CATGTCATGG AAGGTTCTGG CCGGATGGTG GTGACAGCTG TTGGTGTCAA CTCTCAGACT 
GGAATCATCC TTACTCTCTT GGGGGTCAAT GAGGATGACG AAGGGGAGAA AAAGAAGAAA 
GGTAAAAAAC AAGGAGTCCC TGAAAATCGC AACAAAGCAA AGACCCAAGA CGGAGTGGCC 
CTGGAAATCC AGCCACTCAA CAGCCAGGAG GGAATCGACA ATGAGGAAAA GGACAAGAAG 
GCAGTCAAGG TGCCTAAAAA GGAGAAGTCA GTGCTGCAGG GCAAGCTGAC TCGCCTGGCT 
GTTCAGATTG GGAAAGCCGG TCTGCTCATG TCTGCTCTCA CGGTTTTCAT CCTGATTCTA 
TACTTTGTGA TTGACAACTT TGTGATAAAT CGCAGACCAT GGCTCCCTGA GTGTACTCCC 
ATCTACATCC AGTACTTTGT CAAGTTCTTC ATCATCGGCA TCACTGTACT GGTGGTGGCT 
GTGCCAGAGG GGCTGCCTCT GGCTGTCACC ATCTCACTGG CCTACTCTGT GAAGAAAATG 
ATGAAAGACA ATAACCTAGT ACGGCACTTG GATGCTTGTG AGACCATGGG CAACGCCACC 
GCCATCTGCT CTGATAAGAC AGGCACGTTG ACCATGAACC GCATGACTGT GGTACAAGCT 
TATATTGGGG GCATCCATTA CCGTCAAATC CCAAGCCCTG ATGTCTTCCT GCCCAAAGTC 
CTGGACCTCA TTGTCAATGG CATTTCTATC AACAGTGCTT ATACCTCCAA GATTCTGCCT 
CCAGAGAAGG AGGGAGGCCT GCCTCGGCAG GTGGGCAACA AGACCGAGTG TGCTCTGCTA 
GGCTTTGTCA CAGATCTGAA GCAGGATTAT CAGGCTGTGC GTAATGAAGT GCCCGAGGAG 
AAGCTCTACA AGGTGTACAC CTTTAACTCA GTGCGCAAGT CAATGAGCAC CGTCATCAGG 
AATCCCAACG GTGGCTTCCG TATGTACAGC AAGGGCGCCT CTGAGATCAT CTTGCGCAAG 
TGTAATCGAA TCCTGGACCG GAAAGGGGAA GCAGTGCCAT TCAAGAATAA AGACAGAGAT 
GATATGGTAC GCACTGTCAT CGAGCCCATG GCCTGTGATG GACTCCGGAC TATCTGCATA 
GCTTACCGGG ACTTCGATGA CACAGAGCCC TCTTGGGACA ATGAGAATGA GATCCTCACC 
GAACTGACCT GTATCGCGGT GGTGGGCATT GAGGACCCTG TGCGCCCAGA GGTGCCAGAT 
GCTATTGCCA AATGCAAACA AGCTGGCATT ACTGTCAGAA TGGTGACAGG TGACAACATC 
AACACAGCCC GGGCCATTGC CACCAAATGT GGCATTCTGA CACCTGGGGA TGACTTCCTG 
TGCTTAGAAG GCAAAGAATT CAACCGGCTC ATCCGCAACG AGAAAGGCGA GGTAGAGCAA 
GAAAAGCTGG ACAAGATCTG GCCTAAGCTT CGGGTCCTGG CGCGATCTTC TCCCACTGAC 
AAGCACACCC TGGTGAAAGG CATAATTGAC AGCACTGTTG GGGAACACCG GCAGGTCGTG 
GCTGTCACTG GTGATGGCAC AAATGACGGG CCTGCTCTGA AGAAAGCGGA TGTTGGTTTT 
GCCATGGGCA TCGCAGGCAC AGATGTAGCA AAGGAGGCTT CAGACATCAT CCTAACAGAT 
GACAACTTCA CCAGCATTGT GAAGGCAGTG ATGTGGGGAC GAAATGTCTA TGACAGCATC 
TCCAAGTTCC TGCAGTTCCA GCTCACTGTC AATGTGGTGG CCGTGATTGT AGCCTTCACT 
GGAGCCTGTA TCACTCAGGA TTCCCCATTG AAAGCTGTGC AGATGTTGTG GGTTAATCTG 
ATCATGGACA CTTTTGCTTC ATTGGCCCTG GCCACAGAGC CCCCTACGGA ATCTCTGTTG 
AAGCGGCGCC CCTATGGCCG AAATAAGCCT CTGATCTCAC GCACTATGAT GAAGAACATC 
TTGGGCCATG CATTCTATCA GCTCATTGTC ATCTTTATCC TTGTCTTTGC GGGTGAGAAA 
TTCTTTGATA TTGATAGTGG GAGGAAGGCA CCTCTACATT CACCACCCAG CCAGCACTAT 
ACCATTGTTT TTAACACCTT CGTGCTGATG CAGCTCTTCA ATGAAATCAA CTCCCGAAAG 
ATCCATGGAG AGAAGAACGT CTTTTCAGGC ATCTACCGCA ACATTATCTT CTGCTCTGTA 
GTCTTGGGCA CATTCATCTG CCAGATTTTC ATCGTGGAAT TTGGGGGTAA ACCCTTCAGT 
TGTACAAGCC TCAGCCTGTC TCAGTGGCTG TGGTGTCTCT TCATTGGGAT TGGAGAACTT 
CTGTGGGGCC AGTTCATCTC CGCAATACCT ACCCGATCCC TGAAGTTCCT GAAGGAGGCT 
GGGCATGGCA CCACCAAAGA GGAGATCACC AAGGATGCCG AGGGACTGGA TGAGATTGAC 
CATGCTGAGA TGGAGCTGCG CCGAGGCCAG ATCCTCTGGT TCCGGGGCCT GAACCGTATC 
CAGACTCAGA TCGACGTAAT TAACACATTC CAGACGGGAG CCTCTTTTAA GGGAGTCCTA 
AGGCGACAGA ACATGGGTCA ACACCTTGAT GTAAAACTTG TTCCTAGTTC ATCCTATGTA 
GCAGTTGCAC CAGTCAAATC TTCCCCCACC ACTTCTGTTC CTGCTGTTTC ATCTCCTCCT 
ATGGGCAATC AAAGTGGTCA AAGCGTTCCA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATP2B4 ( NM_001365784.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001352713.1 Homo sapiens ATPase plasma membrane Ca2+ transporting 4 (ATP2B4), transcript variant 4, mRNA.

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CloneID	OHu107180
Clone ID Related Accession (Same CDS sequence)	NM_001365784.1 , NM_001365783.1 , NM_001365783.2
Accession Version	NM_001365784.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3495bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-25
Organism	Homo sapiens(human)
Product	plasma membrane calcium-transporting ATPase 4 isoform 4c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL513343.22 and AC114402.2. Transcript Variant: This variant (4), as well as variant 3, encodes isoform 4c. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.86606.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACGAACC CATCAGACCG TGTCTTGCCT GCCAACTCGA TGGCCGAGAG CCGTGAAGGG 
GACTTTGGCT GCACAGTAAT GGAACTGAGG AAGCTCATGG AGCTGCGTTC AAGGGATGCA 
CTGACCCAGA TTAATGTCCA CTATGGAGGT GTACAGAATC TCTGCAGTAG ACTGAAAACC 
TCCCCTGTGG AAGGTCTGTC TGGGAACCCT GCAGATCTGG AGAAACGTAG GCAGGTGTTT 
GGACACAACG TGATCCCCCC CAAAAAGCCC AAGACTTTCT TAGAATTAGT GTGGGAAGCT 
CTTCAAGATG TCACGCTTAT CATCCTGGAG ATTGCAGCCA TCATCTCCCT GGTCCTGTCC 
TTTTATCGCC CTGCTGGTGA AGAAAATGAA CTGTGTGGTC AAGTCGCAAC TACCCCAGAA 
GATGAAAATG AGGCACAAGC TGGCTGGATT GAGGGGGCAG CCATCCTTTT CTCAGTGATC 
ATCGTGGTGT TAGTGACTGC CTTTAATGAT TGGAGCAAAG AGAAGCAATT CCGGGGGCTG 
CAGTGCCGCA TTGAACAGGA GCAAAAGTTC TCCATCATCC GAAACGGTCA ACTCATCCAG 
CTCCCTGTGG CTGAGATTGT GGTTGGTGAT ATTGCCCAAG TCAAATACGG TGATCTGCTG 
CCTGCAGATG GAATCCTGAT CCAAGGGAAT GATCTGAAGA TTGATGAGAG CTCTCTGACA 
GGGGAATCTG ACCATGTCAA GAAGTCCCTG GACAAAGACC CCATGTTGCT CTCAGGGACC 
CATGTCATGG AAGGTTCTGG CCGGATGGTG GTGACAGCTG TTGGTGTCAA CTCTCAGACT 
GGAATCATCC TTACTCTCTT GGGGGTCAAT GAGGATGACG AAGGGGAGAA AAAGAAGAAA 
GGTAAAAAAC AAGGAGTCCC TGAAAATCGC AACAAAGCAA AGACCCAAGA CGGAGTGGCC 
CTGGAAATCC AGCCACTCAA CAGCCAGGAG GGAATCGACA ATGAGGAAAA GGACAAGAAG 
GCAGTCAAGG TGCCTAAAAA GGAGAAGTCA GTGCTGCAGG GCAAGCTGAC TCGCCTGGCT 
GTTCAGATTG GGAAAGCCGG TCTGCTCATG TCTGCTCTCA CGGTTTTCAT CCTGATTCTA 
TACTTTGTGA TTGACAACTT TGTGATAAAT CGCAGACCAT GGCTCCCTGA GTGTACTCCC 
ATCTACATCC AGTACTTTGT CAAGTTCTTC ATCATCGGCA TCACTGTACT GGTGGTGGCT 
GTGCCAGAGG GGCTGCCTCT GGCTGTCACC ATCTCACTGG CCTACTCTGT GAAGAAAATG 
ATGAAAGACA ATAACCTAGT ACGGCACTTG GATGCTTGTG AGACCATGGG CAACGCCACC 
GCCATCTGCT CTGATAAGAC AGGCACGTTG ACCATGAACC GCATGACTGT GGTACAAGCT 
TATATTGGGG GCATCCATTA CCGTCAAATC CCAAGCCCTG ATGTCTTCCT GCCCAAAGTC 
CTGGACCTCA TTGTCAATGG CATTTCTATC AACAGTGCTT ATACCTCCAA GATTCTGCCT 
CCAGAGAAGG AGGGAGGCCT GCCTCGGCAG GTGGGCAACA AGACCGAGTG TGCTCTGCTA 
GGCTTTGTCA CAGATCTGAA GCAGGATTAT CAGGCTGTGC GTAATGAAGT GCCCGAGGAG 
AAGCTCTACA AGGTGTACAC CTTTAACTCA GTGCGCAAGT CAATGAGCAC CGTCATCAGG 
AATCCCAACG GTGGCTTCCG TATGTACAGC AAGGGCGCCT CTGAGATCAT CTTGCGCAAG 
TGTAATCGAA TCCTGGACCG GAAAGGGGAA GCAGTGCCAT TCAAGAATAA AGACAGAGAT 
GATATGGTAC GCACTGTCAT CGAGCCCATG GCCTGTGATG GACTCCGGAC TATCTGCATA 
GCTTACCGGG ACTTCGATGA CACAGAGCCC TCTTGGGACA ATGAGAATGA GATCCTCACC 
GAACTGACCT GTATCGCGGT GGTGGGCATT GAGGACCCTG TGCGCCCAGA GGTGCCAGAT 
GCTATTGCCA AATGCAAACA AGCTGGCATT ACTGTCAGAA TGGTGACAGG TGACAACATC 
AACACAGCCC GGGCCATTGC CACCAAATGT GGCATTCTGA CACCTGGGGA TGACTTCCTG 
TGCTTAGAAG GCAAAGAATT CAACCGGCTC ATCCGCAACG AGAAAGGCGA GGTAGAGCAA 
GAAAAGCTGG ACAAGATCTG GCCTAAGCTT CGGGTCCTGG CGCGATCTTC TCCCACTGAC 
AAGCACACCC TGGTGAAAGG CATAATTGAC AGCACTGTTG GGGAACACCG GCAGGTCGTG 
GCTGTCACTG GTGATGGCAC AAATGACGGG CCTGCTCTGA AGAAAGCGGA TGTTGGTTTT 
GCCATGGGCA TCGCAGGCAC AGATGTAGCA AAGGAGGCTT CAGACATCAT CCTAACAGAT 
GACAACTTCA CCAGCATTGT GAAGGCAGTG ATGTGGGGAC GAAATGTCTA TGACAGCATC 
TCCAAGTTCC TGCAGTTCCA GCTCACTGTC AATGTGGTGG CCGTGATTGT AGCCTTCACT 
GGAGCCTGTA TCACTCAGGA TTCCCCATTG AAAGCTGTGC AGATGTTGTG GGTTAATCTG 
ATCATGGACA CTTTTGCTTC ATTGGCCCTG GCCACAGAGC CCCCTACGGA ATCTCTGTTG 
AAGCGGCGCC CCTATGGCCG AAATAAGCCT CTGATCTCAC GCACTATGAT GAAGAACATC 
TTGGGCCATG CATTCTATCA GCTCATTGTC ATCTTTATCC TTGTCTTTGC GGGTGAGAAA 
TTCTTTGATA TTGATAGTGG GAGGAAGGCA CCTCTACATT CACCACCCAG CCAGCACTAT 
ACCATTGTTT TTAACACCTT CGTGCTGATG CAGCTCTTCA ATGAAATCAA CTCCCGAAAG 
ATCCATGGAG AGAAGAACGT CTTTTCAGGC ATCTACCGCA ACATTATCTT CTGCTCTGTA 
GTCTTGGGCA CATTCATCTG CCAGATTTTC ATCGTGGAAT TTGGGGGTAA ACCCTTCAGT 
TGTACAAGCC TCAGCCTGTC TCAGTGGCTG TGGTGTCTCT TCATTGGGAT TGGAGAACTT 
CTGTGGGGCC AGTTCATCTC CGCAATACCT ACCCGATCCC TGAAGTTCCT GAAGGAGGCT 
GGGCATGGCA CCACCAAAGA GGAGATCACC AAGGATGCCG AGGGACTGGA TGAGATTGAC 
CATGCTGAGA TGGAGCTGCG CCGAGGCCAG ATCCTCTGGT TCCGGGGCCT GAACCGTATC 
CAGACTCAGA TCGACGTAAT TAACACATTC CAGACGGGAG CCTCTTTTAA GGGAGTCCTA 
AGGCGACAGA ACATGGGTCA ACACCTTGAT GTAAAACTTG TTCCTAGTTC ATCCTATGTA 
GCAGTTGCAC CAGTCAAATC TTCCCCCACC ACTTCTGTTC CTGCTGTTTC ATCTCCTCCT 
ATGGGCAGTG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001352713.1
CDS	610..4104
Translation	MTNPSDRVLPANSMAESREGDFGCTVMELRKLMELRSRDALTQINVHYGGVQNLCSRLKTSPVEGLSGNPADLEKRRQVFGHNVIPPKKPKTFLELVWEALQDVTLIILEIAAIISLVLSFYRPAGEENELCGQVATTPEDENEAQAGWIEGAAILFSVIIVVLVTAFNDWSKEKQFRGLQCRIEQEQKFSIIRNGQLIQLPVAEIVVGDIAQVKYGDLLPADGILIQGNDLKIDESSLTGESDHVKKSLDKDPMLLSGTHVMEGSGRMVVTAVGVNSQTGIILTLLGVNEDDEGEKKKKGKKQGVPENRNKAKTQDGVALEIQPLNSQEGIDNEEKDKKAVKVPKKEKSVLQGKLTRLAVQIGKAGLLMSALTVFILILYFVIDNFVINRRPWLPECTPIYIQYFVKFFIIGITVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTMNRMTVVQAYIGGIHYRQIPSPDVFLPKVLDLIVNGISINSAYTSKILPPEKEGGLPRQVGNKTECALLGFVTDLKQDYQAVRNEVPEEKLYKVYTFNSVRKSMSTVIRNPNGGFRMYSKGASEIILRKCNRILDRKGEAVPFKNKDRDDMVRTVIEPMACDGLRTICIAYRDFDDTEPSWDNENEILTELTCIAVVGIEDPVRPEVPDAIAKCKQAGITVRMVTGDNINTARAIATKCGILTPGDDFLCLEGKEFNRLIRNEKGEVEQEKLDKIWPKLRVLARSSPTDKHTLVKGIIDSTVGEHRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFTSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVIVAFTGACITQDSPLKAVQMLWVNLIMDTFASLALATEPPTESLLKRRPYGRNKPLISRTMMKNILGHAFYQLIVIFILVFAGEKFFDIDSGRKAPLHSPPSQHYTIVFNTFVLMQLFNEINSRKIHGEKNVFSGIYRNIIFCSVVLGTFICQIFIVEFGGKPFSCTSLSLSQWLWCLFIGIGELLWGQFISAIPTRSLKFLKEAGHGTTKEEITKDAEGLDEIDHAEMELRRGQILWFRGLNRIQTQIDVINTFQTGASFKGVLRRQNMGQHLDVKLVPSSSYVAVAPVKSSPTTSVPAVSSPPMGSE

Target ORF information:

RefSeq Version	NM_001365784.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ATPase plasma membrane Ca2+ transporting 4 (ATP2B4), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001365784.1

ORF Insert Sequence:

ATGACGAACC CATCAGACCG TGTCTTGCCT GCCAACTCGA TGGCCGAGAG CCGTGAAGGG 
GACTTTGGCT GCACAGTAAT GGAACTGAGG AAGCTCATGG AGCTGCGTTC AAGGGATGCA 
CTGACCCAGA TTAATGTCCA CTATGGAGGT GTACAGAATC TCTGCAGTAG ACTGAAAACC 
TCCCCTGTGG AAGGTCTGTC TGGGAACCCT GCAGATCTGG AGAAACGTAG GCAGGTGTTT 
GGACACAACG TGATCCCCCC CAAAAAGCCC AAGACTTTCT TAGAATTAGT GTGGGAAGCT 
CTTCAAGATG TCACGCTTAT CATCCTGGAG ATTGCAGCCA TCATCTCCCT GGTCCTGTCC 
TTTTATCGCC CTGCTGGTGA AGAAAATGAA CTGTGTGGTC AAGTCGCAAC TACCCCAGAA 
GATGAAAATG AGGCACAAGC TGGCTGGATT GAGGGGGCAG CCATCCTTTT CTCAGTGATC 
ATCGTGGTGT TAGTGACTGC CTTTAATGAT TGGAGCAAAG AGAAGCAATT CCGGGGGCTG 
CAGTGCCGCA TTGAACAGGA GCAAAAGTTC TCCATCATCC GAAACGGTCA ACTCATCCAG 
CTCCCTGTGG CTGAGATTGT GGTTGGTGAT ATTGCCCAAG TCAAATACGG TGATCTGCTG 
CCTGCAGATG GAATCCTGAT CCAAGGGAAT GATCTGAAGA TTGATGAGAG CTCTCTGACA 
GGGGAATCTG ACCATGTCAA GAAGTCCCTG GACAAAGACC CCATGTTGCT CTCAGGGACC 
CATGTCATGG AAGGTTCTGG CCGGATGGTG GTGACAGCTG TTGGTGTCAA CTCTCAGACT 
GGAATCATCC TTACTCTCTT GGGGGTCAAT GAGGATGACG AAGGGGAGAA AAAGAAGAAA 
GGTAAAAAAC AAGGAGTCCC TGAAAATCGC AACAAAGCAA AGACCCAAGA CGGAGTGGCC 
CTGGAAATCC AGCCACTCAA CAGCCAGGAG GGAATCGACA ATGAGGAAAA GGACAAGAAG 
GCAGTCAAGG TGCCTAAAAA GGAGAAGTCA GTGCTGCAGG GCAAGCTGAC TCGCCTGGCT 
GTTCAGATTG GGAAAGCCGG TCTGCTCATG TCTGCTCTCA CGGTTTTCAT CCTGATTCTA 
TACTTTGTGA TTGACAACTT TGTGATAAAT CGCAGACCAT GGCTCCCTGA GTGTACTCCC 
ATCTACATCC AGTACTTTGT CAAGTTCTTC ATCATCGGCA TCACTGTACT GGTGGTGGCT 
GTGCCAGAGG GGCTGCCTCT GGCTGTCACC ATCTCACTGG CCTACTCTGT GAAGAAAATG 
ATGAAAGACA ATAACCTAGT ACGGCACTTG GATGCTTGTG AGACCATGGG CAACGCCACC 
GCCATCTGCT CTGATAAGAC AGGCACGTTG ACCATGAACC GCATGACTGT GGTACAAGCT 
TATATTGGGG GCATCCATTA CCGTCAAATC CCAAGCCCTG ATGTCTTCCT GCCCAAAGTC 
CTGGACCTCA TTGTCAATGG CATTTCTATC AACAGTGCTT ATACCTCCAA GATTCTGCCT 
CCAGAGAAGG AGGGAGGCCT GCCTCGGCAG GTGGGCAACA AGACCGAGTG TGCTCTGCTA 
GGCTTTGTCA CAGATCTGAA GCAGGATTAT CAGGCTGTGC GTAATGAAGT GCCCGAGGAG 
AAGCTCTACA AGGTGTACAC CTTTAACTCA GTGCGCAAGT CAATGAGCAC CGTCATCAGG 
AATCCCAACG GTGGCTTCCG TATGTACAGC AAGGGCGCCT CTGAGATCAT CTTGCGCAAG 
TGTAATCGAA TCCTGGACCG GAAAGGGGAA GCAGTGCCAT TCAAGAATAA AGACAGAGAT 
GATATGGTAC GCACTGTCAT CGAGCCCATG GCCTGTGATG GACTCCGGAC TATCTGCATA 
GCTTACCGGG ACTTCGATGA CACAGAGCCC TCTTGGGACA ATGAGAATGA GATCCTCACC 
GAACTGACCT GTATCGCGGT GGTGGGCATT GAGGACCCTG TGCGCCCAGA GGTGCCAGAT 
GCTATTGCCA AATGCAAACA AGCTGGCATT ACTGTCAGAA TGGTGACAGG TGACAACATC 
AACACAGCCC GGGCCATTGC CACCAAATGT GGCATTCTGA CACCTGGGGA TGACTTCCTG 
TGCTTAGAAG GCAAAGAATT CAACCGGCTC ATCCGCAACG AGAAAGGCGA GGTAGAGCAA 
GAAAAGCTGG ACAAGATCTG GCCTAAGCTT CGGGTCCTGG CGCGATCTTC TCCCACTGAC 
AAGCACACCC TGGTGAAAGG CATAATTGAC AGCACTGTTG GGGAACACCG GCAGGTCGTG 
GCTGTCACTG GTGATGGCAC AAATGACGGG CCTGCTCTGA AGAAAGCGGA TGTTGGTTTT 
GCCATGGGCA TCGCAGGCAC AGATGTAGCA AAGGAGGCTT CAGACATCAT CCTAACAGAT 
GACAACTTCA CCAGCATTGT GAAGGCAGTG ATGTGGGGAC GAAATGTCTA TGACAGCATC 
TCCAAGTTCC TGCAGTTCCA GCTCACTGTC AATGTGGTGG CCGTGATTGT AGCCTTCACT 
GGAGCCTGTA TCACTCAGGA TTCCCCATTG AAAGCTGTGC AGATGTTGTG GGTTAATCTG 
ATCATGGACA CTTTTGCTTC ATTGGCCCTG GCCACAGAGC CCCCTACGGA ATCTCTGTTG 
AAGCGGCGCC CCTATGGCCG AAATAAGCCT CTGATCTCAC GCACTATGAT GAAGAACATC 
TTGGGCCATG CATTCTATCA GCTCATTGTC ATCTTTATCC TTGTCTTTGC GGGTGAGAAA 
TTCTTTGATA TTGATAGTGG GAGGAAGGCA CCTCTACATT CACCACCCAG CCAGCACTAT 
ACCATTGTTT TTAACACCTT CGTGCTGATG CAGCTCTTCA ATGAAATCAA CTCCCGAAAG 
ATCCATGGAG AGAAGAACGT CTTTTCAGGC ATCTACCGCA ACATTATCTT CTGCTCTGTA 
GTCTTGGGCA CATTCATCTG CCAGATTTTC ATCGTGGAAT TTGGGGGTAA ACCCTTCAGT 
TGTACAAGCC TCAGCCTGTC TCAGTGGCTG TGGTGTCTCT TCATTGGGAT TGGAGAACTT 
CTGTGGGGCC AGTTCATCTC CGCAATACCT ACCCGATCCC TGAAGTTCCT GAAGGAGGCT 
GGGCATGGCA CCACCAAAGA GGAGATCACC AAGGATGCCG AGGGACTGGA TGAGATTGAC 
CATGCTGAGA TGGAGCTGCG CCGAGGCCAG ATCCTCTGGT TCCGGGGCCT GAACCGTATC 
CAGACTCAGA TCGACGTAAT TAACACATTC CAGACGGGAG CCTCTTTTAA GGGAGTCCTA 
AGGCGACAGA ACATGGGTCA ACACCTTGAT GTAAAACTTG TTCCTAGTTC ATCCTATGTA 
GCAGTTGCAC CAGTCAAATC TTCCCCCACC ACTTCTGTTC CTGCTGTTTC ATCTCCTCCT 
ATGGGCAGTG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATP2B4 ( NM_001365783.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001352712.1 Homo sapiens ATPase plasma membrane Ca2+ transporting 4 (ATP2B4), transcript variant 3, mRNA.

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CloneID	OHu107180
Clone ID Related Accession (Same CDS sequence)	NM_001365784.1 , NM_001365783.1 , NM_001365783.2
Accession Version	NM_001365783.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3495bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Homo sapiens(human)
Product	plasma membrane calcium-transporting ATPase 4 isoform 4c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL513343.22 and AC114402.2. Transcript Variant: This variant (3), as well as variant 4, encodes isoform 4c. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.21475.1, SRR1803613.150083.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACGAACC CATCAGACCG TGTCTTGCCT GCCAACTCGA TGGCCGAGAG CCGTGAAGGG 
GACTTTGGCT GCACAGTAAT GGAACTGAGG AAGCTCATGG AGCTGCGTTC AAGGGATGCA 
CTGACCCAGA TTAATGTCCA CTATGGAGGT GTACAGAATC TCTGCAGTAG ACTGAAAACC 
TCCCCTGTGG AAGGTCTGTC TGGGAACCCT GCAGATCTGG AGAAACGTAG GCAGGTGTTT 
GGACACAACG TGATCCCCCC CAAAAAGCCC AAGACTTTCT TAGAATTAGT GTGGGAAGCT 
CTTCAAGATG TCACGCTTAT CATCCTGGAG ATTGCAGCCA TCATCTCCCT GGTCCTGTCC 
TTTTATCGCC CTGCTGGTGA AGAAAATGAA CTGTGTGGTC AAGTCGCAAC TACCCCAGAA 
GATGAAAATG AGGCACAAGC TGGCTGGATT GAGGGGGCAG CCATCCTTTT CTCAGTGATC 
ATCGTGGTGT TAGTGACTGC CTTTAATGAT TGGAGCAAAG AGAAGCAATT CCGGGGGCTG 
CAGTGCCGCA TTGAACAGGA GCAAAAGTTC TCCATCATCC GAAACGGTCA ACTCATCCAG 
CTCCCTGTGG CTGAGATTGT GGTTGGTGAT ATTGCCCAAG TCAAATACGG TGATCTGCTG 
CCTGCAGATG GAATCCTGAT CCAAGGGAAT GATCTGAAGA TTGATGAGAG CTCTCTGACA 
GGGGAATCTG ACCATGTCAA GAAGTCCCTG GACAAAGACC CCATGTTGCT CTCAGGGACC 
CATGTCATGG AAGGTTCTGG CCGGATGGTG GTGACAGCTG TTGGTGTCAA CTCTCAGACT 
GGAATCATCC TTACTCTCTT GGGGGTCAAT GAGGATGACG AAGGGGAGAA AAAGAAGAAA 
GGTAAAAAAC AAGGAGTCCC TGAAAATCGC AACAAAGCAA AGACCCAAGA CGGAGTGGCC 
CTGGAAATCC AGCCACTCAA CAGCCAGGAG GGAATCGACA ATGAGGAAAA GGACAAGAAG 
GCAGTCAAGG TGCCTAAAAA GGAGAAGTCA GTGCTGCAGG GCAAGCTGAC TCGCCTGGCT 
GTTCAGATTG GGAAAGCCGG TCTGCTCATG TCTGCTCTCA CGGTTTTCAT CCTGATTCTA 
TACTTTGTGA TTGACAACTT TGTGATAAAT CGCAGACCAT GGCTCCCTGA GTGTACTCCC 
ATCTACATCC AGTACTTTGT CAAGTTCTTC ATCATCGGCA TCACTGTACT GGTGGTGGCT 
GTGCCAGAGG GGCTGCCTCT GGCTGTCACC ATCTCACTGG CCTACTCTGT GAAGAAAATG 
ATGAAAGACA ATAACCTAGT ACGGCACTTG GATGCTTGTG AGACCATGGG CAACGCCACC 
GCCATCTGCT CTGATAAGAC AGGCACGTTG ACCATGAACC GCATGACTGT GGTACAAGCT 
TATATTGGGG GCATCCATTA CCGTCAAATC CCAAGCCCTG ATGTCTTCCT GCCCAAAGTC 
CTGGACCTCA TTGTCAATGG CATTTCTATC AACAGTGCTT ATACCTCCAA GATTCTGCCT 
CCAGAGAAGG AGGGAGGCCT GCCTCGGCAG GTGGGCAACA AGACCGAGTG TGCTCTGCTA 
GGCTTTGTCA CAGATCTGAA GCAGGATTAT CAGGCTGTGC GTAATGAAGT GCCCGAGGAG 
AAGCTCTACA AGGTGTACAC CTTTAACTCA GTGCGCAAGT CAATGAGCAC CGTCATCAGG 
AATCCCAACG GTGGCTTCCG TATGTACAGC AAGGGCGCCT CTGAGATCAT CTTGCGCAAG 
TGTAATCGAA TCCTGGACCG GAAAGGGGAA GCAGTGCCAT TCAAGAATAA AGACAGAGAT 
GATATGGTAC GCACTGTCAT CGAGCCCATG GCCTGTGATG GACTCCGGAC TATCTGCATA 
GCTTACCGGG ACTTCGATGA CACAGAGCCC TCTTGGGACA ATGAGAATGA GATCCTCACC 
GAACTGACCT GTATCGCGGT GGTGGGCATT GAGGACCCTG TGCGCCCAGA GGTGCCAGAT 
GCTATTGCCA AATGCAAACA AGCTGGCATT ACTGTCAGAA TGGTGACAGG TGACAACATC 
AACACAGCCC GGGCCATTGC CACCAAATGT GGCATTCTGA CACCTGGGGA TGACTTCCTG 
TGCTTAGAAG GCAAAGAATT CAACCGGCTC ATCCGCAACG AGAAAGGCGA GGTAGAGCAA 
GAAAAGCTGG ACAAGATCTG GCCTAAGCTT CGGGTCCTGG CGCGATCTTC TCCCACTGAC 
AAGCACACCC TGGTGAAAGG CATAATTGAC AGCACTGTTG GGGAACACCG GCAGGTCGTG 
GCTGTCACTG GTGATGGCAC AAATGACGGG CCTGCTCTGA AGAAAGCGGA TGTTGGTTTT 
GCCATGGGCA TCGCAGGCAC AGATGTAGCA AAGGAGGCTT CAGACATCAT CCTAACAGAT 
GACAACTTCA CCAGCATTGT GAAGGCAGTG ATGTGGGGAC GAAATGTCTA TGACAGCATC 
TCCAAGTTCC TGCAGTTCCA GCTCACTGTC AATGTGGTGG CCGTGATTGT AGCCTTCACT 
GGAGCCTGTA TCACTCAGGA TTCCCCATTG AAAGCTGTGC AGATGTTGTG GGTTAATCTG 
ATCATGGACA CTTTTGCTTC ATTGGCCCTG GCCACAGAGC CCCCTACGGA ATCTCTGTTG 
AAGCGGCGCC CCTATGGCCG AAATAAGCCT CTGATCTCAC GCACTATGAT GAAGAACATC 
TTGGGCCATG CATTCTATCA GCTCATTGTC ATCTTTATCC TTGTCTTTGC GGGTGAGAAA 
TTCTTTGATA TTGATAGTGG GAGGAAGGCA CCTCTACATT CACCACCCAG CCAGCACTAT 
ACCATTGTTT TTAACACCTT CGTGCTGATG CAGCTCTTCA ATGAAATCAA CTCCCGAAAG 
ATCCATGGAG AGAAGAACGT CTTTTCAGGC ATCTACCGCA ACATTATCTT CTGCTCTGTA 
GTCTTGGGCA CATTCATCTG CCAGATTTTC ATCGTGGAAT TTGGGGGTAA ACCCTTCAGT 
TGTACAAGCC TCAGCCTGTC TCAGTGGCTG TGGTGTCTCT TCATTGGGAT TGGAGAACTT 
CTGTGGGGCC AGTTCATCTC CGCAATACCT ACCCGATCCC TGAAGTTCCT GAAGGAGGCT 
GGGCATGGCA CCACCAAAGA GGAGATCACC AAGGATGCCG AGGGACTGGA TGAGATTGAC 
CATGCTGAGA TGGAGCTGCG CCGAGGCCAG ATCCTCTGGT TCCGGGGCCT GAACCGTATC 
CAGACTCAGA TCGACGTAAT TAACACATTC CAGACGGGAG CCTCTTTTAA GGGAGTCCTA 
AGGCGACAGA ACATGGGTCA ACACCTTGAT GTAAAACTTG TTCCTAGTTC ATCCTATGTA 
GCAGTTGCAC CAGTCAAATC TTCCCCCACC ACTTCTGTTC CTGCTGTTTC ATCTCCTCCT 
ATGGGCAGTG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001352712.1
CDS	853..4347
Translation	MTNPSDRVLPANSMAESREGDFGCTVMELRKLMELRSRDALTQINVHYGGVQNLCSRLKTSPVEGLSGNPADLEKRRQVFGHNVIPPKKPKTFLELVWEALQDVTLIILEIAAIISLVLSFYRPAGEENELCGQVATTPEDENEAQAGWIEGAAILFSVIIVVLVTAFNDWSKEKQFRGLQCRIEQEQKFSIIRNGQLIQLPVAEIVVGDIAQVKYGDLLPADGILIQGNDLKIDESSLTGESDHVKKSLDKDPMLLSGTHVMEGSGRMVVTAVGVNSQTGIILTLLGVNEDDEGEKKKKGKKQGVPENRNKAKTQDGVALEIQPLNSQEGIDNEEKDKKAVKVPKKEKSVLQGKLTRLAVQIGKAGLLMSALTVFILILYFVIDNFVINRRPWLPECTPIYIQYFVKFFIIGITVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTMNRMTVVQAYIGGIHYRQIPSPDVFLPKVLDLIVNGISINSAYTSKILPPEKEGGLPRQVGNKTECALLGFVTDLKQDYQAVRNEVPEEKLYKVYTFNSVRKSMSTVIRNPNGGFRMYSKGASEIILRKCNRILDRKGEAVPFKNKDRDDMVRTVIEPMACDGLRTICIAYRDFDDTEPSWDNENEILTELTCIAVVGIEDPVRPEVPDAIAKCKQAGITVRMVTGDNINTARAIATKCGILTPGDDFLCLEGKEFNRLIRNEKGEVEQEKLDKIWPKLRVLARSSPTDKHTLVKGIIDSTVGEHRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFTSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVIVAFTGACITQDSPLKAVQMLWVNLIMDTFASLALATEPPTESLLKRRPYGRNKPLISRTMMKNILGHAFYQLIVIFILVFAGEKFFDIDSGRKAPLHSPPSQHYTIVFNTFVLMQLFNEINSRKIHGEKNVFSGIYRNIIFCSVVLGTFICQIFIVEFGGKPFSCTSLSLSQWLWCLFIGIGELLWGQFISAIPTRSLKFLKEAGHGTTKEEITKDAEGLDEIDHAEMELRRGQILWFRGLNRIQTQIDVINTFQTGASFKGVLRRQNMGQHLDVKLVPSSSYVAVAPVKSSPTTSVPAVSSPPMGSE

Target ORF information:

RefSeq Version	NM_001365783.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ATPase plasma membrane Ca2+ transporting 4 (ATP2B4), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001365783.1

ORF Insert Sequence:

ATGACGAACC CATCAGACCG TGTCTTGCCT GCCAACTCGA TGGCCGAGAG CCGTGAAGGG 
GACTTTGGCT GCACAGTAAT GGAACTGAGG AAGCTCATGG AGCTGCGTTC AAGGGATGCA 
CTGACCCAGA TTAATGTCCA CTATGGAGGT GTACAGAATC TCTGCAGTAG ACTGAAAACC 
TCCCCTGTGG AAGGTCTGTC TGGGAACCCT GCAGATCTGG AGAAACGTAG GCAGGTGTTT 
GGACACAACG TGATCCCCCC CAAAAAGCCC AAGACTTTCT TAGAATTAGT GTGGGAAGCT 
CTTCAAGATG TCACGCTTAT CATCCTGGAG ATTGCAGCCA TCATCTCCCT GGTCCTGTCC 
TTTTATCGCC CTGCTGGTGA AGAAAATGAA CTGTGTGGTC AAGTCGCAAC TACCCCAGAA 
GATGAAAATG AGGCACAAGC TGGCTGGATT GAGGGGGCAG CCATCCTTTT CTCAGTGATC 
ATCGTGGTGT TAGTGACTGC CTTTAATGAT TGGAGCAAAG AGAAGCAATT CCGGGGGCTG 
CAGTGCCGCA TTGAACAGGA GCAAAAGTTC TCCATCATCC GAAACGGTCA ACTCATCCAG 
CTCCCTGTGG CTGAGATTGT GGTTGGTGAT ATTGCCCAAG TCAAATACGG TGATCTGCTG 
CCTGCAGATG GAATCCTGAT CCAAGGGAAT GATCTGAAGA TTGATGAGAG CTCTCTGACA 
GGGGAATCTG ACCATGTCAA GAAGTCCCTG GACAAAGACC CCATGTTGCT CTCAGGGACC 
CATGTCATGG AAGGTTCTGG CCGGATGGTG GTGACAGCTG TTGGTGTCAA CTCTCAGACT 
GGAATCATCC TTACTCTCTT GGGGGTCAAT GAGGATGACG AAGGGGAGAA AAAGAAGAAA 
GGTAAAAAAC AAGGAGTCCC TGAAAATCGC AACAAAGCAA AGACCCAAGA CGGAGTGGCC 
CTGGAAATCC AGCCACTCAA CAGCCAGGAG GGAATCGACA ATGAGGAAAA GGACAAGAAG 
GCAGTCAAGG TGCCTAAAAA GGAGAAGTCA GTGCTGCAGG GCAAGCTGAC TCGCCTGGCT 
GTTCAGATTG GGAAAGCCGG TCTGCTCATG TCTGCTCTCA CGGTTTTCAT CCTGATTCTA 
TACTTTGTGA TTGACAACTT TGTGATAAAT CGCAGACCAT GGCTCCCTGA GTGTACTCCC 
ATCTACATCC AGTACTTTGT CAAGTTCTTC ATCATCGGCA TCACTGTACT GGTGGTGGCT 
GTGCCAGAGG GGCTGCCTCT GGCTGTCACC ATCTCACTGG CCTACTCTGT GAAGAAAATG 
ATGAAAGACA ATAACCTAGT ACGGCACTTG GATGCTTGTG AGACCATGGG CAACGCCACC 
GCCATCTGCT CTGATAAGAC AGGCACGTTG ACCATGAACC GCATGACTGT GGTACAAGCT 
TATATTGGGG GCATCCATTA CCGTCAAATC CCAAGCCCTG ATGTCTTCCT GCCCAAAGTC 
CTGGACCTCA TTGTCAATGG CATTTCTATC AACAGTGCTT ATACCTCCAA GATTCTGCCT 
CCAGAGAAGG AGGGAGGCCT GCCTCGGCAG GTGGGCAACA AGACCGAGTG TGCTCTGCTA 
GGCTTTGTCA CAGATCTGAA GCAGGATTAT CAGGCTGTGC GTAATGAAGT GCCCGAGGAG 
AAGCTCTACA AGGTGTACAC CTTTAACTCA GTGCGCAAGT CAATGAGCAC CGTCATCAGG 
AATCCCAACG GTGGCTTCCG TATGTACAGC AAGGGCGCCT CTGAGATCAT CTTGCGCAAG 
TGTAATCGAA TCCTGGACCG GAAAGGGGAA GCAGTGCCAT TCAAGAATAA AGACAGAGAT 
GATATGGTAC GCACTGTCAT CGAGCCCATG GCCTGTGATG GACTCCGGAC TATCTGCATA 
GCTTACCGGG ACTTCGATGA CACAGAGCCC TCTTGGGACA ATGAGAATGA GATCCTCACC 
GAACTGACCT GTATCGCGGT GGTGGGCATT GAGGACCCTG TGCGCCCAGA GGTGCCAGAT 
GCTATTGCCA AATGCAAACA AGCTGGCATT ACTGTCAGAA TGGTGACAGG TGACAACATC 
AACACAGCCC GGGCCATTGC CACCAAATGT GGCATTCTGA CACCTGGGGA TGACTTCCTG 
TGCTTAGAAG GCAAAGAATT CAACCGGCTC ATCCGCAACG AGAAAGGCGA GGTAGAGCAA 
GAAAAGCTGG ACAAGATCTG GCCTAAGCTT CGGGTCCTGG CGCGATCTTC TCCCACTGAC 
AAGCACACCC TGGTGAAAGG CATAATTGAC AGCACTGTTG GGGAACACCG GCAGGTCGTG 
GCTGTCACTG GTGATGGCAC AAATGACGGG CCTGCTCTGA AGAAAGCGGA TGTTGGTTTT 
GCCATGGGCA TCGCAGGCAC AGATGTAGCA AAGGAGGCTT CAGACATCAT CCTAACAGAT 
GACAACTTCA CCAGCATTGT GAAGGCAGTG ATGTGGGGAC GAAATGTCTA TGACAGCATC 
TCCAAGTTCC TGCAGTTCCA GCTCACTGTC AATGTGGTGG CCGTGATTGT AGCCTTCACT 
GGAGCCTGTA TCACTCAGGA TTCCCCATTG AAAGCTGTGC AGATGTTGTG GGTTAATCTG 
ATCATGGACA CTTTTGCTTC ATTGGCCCTG GCCACAGAGC CCCCTACGGA ATCTCTGTTG 
AAGCGGCGCC CCTATGGCCG AAATAAGCCT CTGATCTCAC GCACTATGAT GAAGAACATC 
TTGGGCCATG CATTCTATCA GCTCATTGTC ATCTTTATCC TTGTCTTTGC GGGTGAGAAA 
TTCTTTGATA TTGATAGTGG GAGGAAGGCA CCTCTACATT CACCACCCAG CCAGCACTAT 
ACCATTGTTT TTAACACCTT CGTGCTGATG CAGCTCTTCA ATGAAATCAA CTCCCGAAAG 
ATCCATGGAG AGAAGAACGT CTTTTCAGGC ATCTACCGCA ACATTATCTT CTGCTCTGTA 
GTCTTGGGCA CATTCATCTG CCAGATTTTC ATCGTGGAAT TTGGGGGTAA ACCCTTCAGT 
TGTACAAGCC TCAGCCTGTC TCAGTGGCTG TGGTGTCTCT TCATTGGGAT TGGAGAACTT 
CTGTGGGGCC AGTTCATCTC CGCAATACCT ACCCGATCCC TGAAGTTCCT GAAGGAGGCT 
GGGCATGGCA CCACCAAAGA GGAGATCACC AAGGATGCCG AGGGACTGGA TGAGATTGAC 
CATGCTGAGA TGGAGCTGCG CCGAGGCCAG ATCCTCTGGT TCCGGGGCCT GAACCGTATC 
CAGACTCAGA TCGACGTAAT TAACACATTC CAGACGGGAG CCTCTTTTAA GGGAGTCCTA 
AGGCGACAGA ACATGGGTCA ACACCTTGAT GTAAAACTTG TTCCTAGTTC ATCCTATGTA 
GCAGTTGCAC CAGTCAAATC TTCCCCCACC ACTTCTGTTC CTGCTGTTTC ATCTCCTCCT 
ATGGGCAGTG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATP2B4 ( NM_001365783.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001352712.1 Homo sapiens ATPase plasma membrane Ca2+ transporting 4 (ATP2B4), transcript variant 3, mRNA.

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CloneID	OHu107180
Clone ID Related Accession (Same CDS sequence)	NM_001365784.1 , NM_001365783.1 , NM_001365783.2
Accession Version	NM_001365783.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3495bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-28
Organism	Homo sapiens(human)
Product	plasma membrane calcium-transporting ATPase 4 isoform 4c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL513343.22 and AC114402.2. On Jun 2, 2019 this sequence version replaced NM_001365783.1. Transcript Variant: This variant (3), as well as variant 4, encodes isoform 4c. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.174745.1, SRR1803613.150083.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACGAACC CATCAGACCG TGTCTTGCCT GCCAACTCGA TGGCCGAGAG CCGTGAAGGG 
GACTTTGGCT GCACAGTAAT GGAACTGAGG AAGCTCATGG AGCTGCGTTC AAGGGATGCA 
CTGACCCAGA TTAATGTCCA CTATGGAGGT GTACAGAATC TCTGCAGTAG ACTGAAAACC 
TCCCCTGTGG AAGGTCTGTC TGGGAACCCT GCAGATCTGG AGAAACGTAG GCAGGTGTTT 
GGACACAACG TGATCCCCCC CAAAAAGCCC AAGACTTTCT TAGAATTAGT GTGGGAAGCT 
CTTCAAGATG TCACGCTTAT CATCCTGGAG ATTGCAGCCA TCATCTCCCT GGTCCTGTCC 
TTTTATCGCC CTGCTGGTGA AGAAAATGAA CTGTGTGGTC AAGTCGCAAC TACCCCAGAA 
GATGAAAATG AGGCACAAGC TGGCTGGATT GAGGGGGCAG CCATCCTTTT CTCAGTGATC 
ATCGTGGTGT TAGTGACTGC CTTTAATGAT TGGAGCAAAG AGAAGCAATT CCGGGGGCTG 
CAGTGCCGCA TTGAACAGGA GCAAAAGTTC TCCATCATCC GAAACGGTCA ACTCATCCAG 
CTCCCTGTGG CTGAGATTGT GGTTGGTGAT ATTGCCCAAG TCAAATACGG TGATCTGCTG 
CCTGCAGATG GAATCCTGAT CCAAGGGAAT GATCTGAAGA TTGATGAGAG CTCTCTGACA 
GGGGAATCTG ACCATGTCAA GAAGTCCCTG GACAAAGACC CCATGTTGCT CTCAGGGACC 
CATGTCATGG AAGGTTCTGG CCGGATGGTG GTGACAGCTG TTGGTGTCAA CTCTCAGACT 
GGAATCATCC TTACTCTCTT GGGGGTCAAT GAGGATGACG AAGGGGAGAA AAAGAAGAAA 
GGTAAAAAAC AAGGAGTCCC TGAAAATCGC AACAAAGCAA AGACCCAAGA CGGAGTGGCC 
CTGGAAATCC AGCCACTCAA CAGCCAGGAG GGAATCGACA ATGAGGAAAA GGACAAGAAG 
GCAGTCAAGG TGCCTAAAAA GGAGAAGTCA GTGCTGCAGG GCAAGCTGAC TCGCCTGGCT 
GTTCAGATTG GGAAAGCCGG TCTGCTCATG TCTGCTCTCA CGGTTTTCAT CCTGATTCTA 
TACTTTGTGA TTGACAACTT TGTGATAAAT CGCAGACCAT GGCTCCCTGA GTGTACTCCC 
ATCTACATCC AGTACTTTGT CAAGTTCTTC ATCATCGGCA TCACTGTACT GGTGGTGGCT 
GTGCCAGAGG GGCTGCCTCT GGCTGTCACC ATCTCACTGG CCTACTCTGT GAAGAAAATG 
ATGAAAGACA ATAACCTAGT ACGGCACTTG GATGCTTGTG AGACCATGGG CAACGCCACC 
GCCATCTGCT CTGATAAGAC AGGCACGTTG ACCATGAACC GCATGACTGT GGTACAAGCT 
TATATTGGGG GCATCCATTA CCGTCAAATC CCAAGCCCTG ATGTCTTCCT GCCCAAAGTC 
CTGGACCTCA TTGTCAATGG CATTTCTATC AACAGTGCTT ATACCTCCAA GATTCTGCCT 
CCAGAGAAGG AGGGAGGCCT GCCTCGGCAG GTGGGCAACA AGACCGAGTG TGCTCTGCTA 
GGCTTTGTCA CAGATCTGAA GCAGGATTAT CAGGCTGTGC GTAATGAAGT GCCCGAGGAG 
AAGCTCTACA AGGTGTACAC CTTTAACTCA GTGCGCAAGT CAATGAGCAC CGTCATCAGG 
AATCCCAACG GTGGCTTCCG TATGTACAGC AAGGGCGCCT CTGAGATCAT CTTGCGCAAG 
TGTAATCGAA TCCTGGACCG GAAAGGGGAA GCAGTGCCAT TCAAGAATAA AGACAGAGAT 
GATATGGTAC GCACTGTCAT CGAGCCCATG GCCTGTGATG GACTCCGGAC TATCTGCATA 
GCTTACCGGG ACTTCGATGA CACAGAGCCC TCTTGGGACA ATGAGAATGA GATCCTCACC 
GAACTGACCT GTATCGCGGT GGTGGGCATT GAGGACCCTG TGCGCCCAGA GGTGCCAGAT 
GCTATTGCCA AATGCAAACA AGCTGGCATT ACTGTCAGAA TGGTGACAGG TGACAACATC 
AACACAGCCC GGGCCATTGC CACCAAATGT GGCATTCTGA CACCTGGGGA TGACTTCCTG 
TGCTTAGAAG GCAAAGAATT CAACCGGCTC ATCCGCAACG AGAAAGGCGA GGTAGAGCAA 
GAAAAGCTGG ACAAGATCTG GCCTAAGCTT CGGGTCCTGG CGCGATCTTC TCCCACTGAC 
AAGCACACCC TGGTGAAAGG CATAATTGAC AGCACTGTTG GGGAACACCG GCAGGTCGTG 
GCTGTCACTG GTGATGGCAC AAATGACGGG CCTGCTCTGA AGAAAGCGGA TGTTGGTTTT 
GCCATGGGCA TCGCAGGCAC AGATGTAGCA AAGGAGGCTT CAGACATCAT CCTAACAGAT 
GACAACTTCA CCAGCATTGT GAAGGCAGTG ATGTGGGGAC GAAATGTCTA TGACAGCATC 
TCCAAGTTCC TGCAGTTCCA GCTCACTGTC AATGTGGTGG CCGTGATTGT AGCCTTCACT 
GGAGCCTGTA TCACTCAGGA TTCCCCATTG AAAGCTGTGC AGATGTTGTG GGTTAATCTG 
ATCATGGACA CTTTTGCTTC ATTGGCCCTG GCCACAGAGC CCCCTACGGA ATCTCTGTTG 
AAGCGGCGCC CCTATGGCCG AAATAAGCCT CTGATCTCAC GCACTATGAT GAAGAACATC 
TTGGGCCATG CATTCTATCA GCTCATTGTC ATCTTTATCC TTGTCTTTGC GGGTGAGAAA 
TTCTTTGATA TTGATAGTGG GAGGAAGGCA CCTCTACATT CACCACCCAG CCAGCACTAT 
ACCATTGTTT TTAACACCTT CGTGCTGATG CAGCTCTTCA ATGAAATCAA CTCCCGAAAG 
ATCCATGGAG AGAAGAACGT CTTTTCAGGC ATCTACCGCA ACATTATCTT CTGCTCTGTA 
GTCTTGGGCA CATTCATCTG CCAGATTTTC ATCGTGGAAT TTGGGGGTAA ACCCTTCAGT 
TGTACAAGCC TCAGCCTGTC TCAGTGGCTG TGGTGTCTCT TCATTGGGAT TGGAGAACTT 
CTGTGGGGCC AGTTCATCTC CGCAATACCT ACCCGATCCC TGAAGTTCCT GAAGGAGGCT 
GGGCATGGCA CCACCAAAGA GGAGATCACC AAGGATGCCG AGGGACTGGA TGAGATTGAC 
CATGCTGAGA TGGAGCTGCG CCGAGGCCAG ATCCTCTGGT TCCGGGGCCT GAACCGTATC 
CAGACTCAGA TCGACGTAAT TAACACATTC CAGACGGGAG CCTCTTTTAA GGGAGTCCTA 
AGGCGACAGA ACATGGGTCA ACACCTTGAT GTAAAACTTG TTCCTAGTTC ATCCTATGTA 
GCAGTTGCAC CAGTCAAATC TTCCCCCACC ACTTCTGTTC CTGCTGTTTC ATCTCCTCCT 
ATGGGCAGTG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001352712.1
CDS	853..4347
Translation	MTNPSDRVLPANSMAESREGDFGCTVMELRKLMELRSRDALTQINVHYGGVQNLCSRLKTSPVEGLSGNPADLEKRRQVFGHNVIPPKKPKTFLELVWEALQDVTLIILEIAAIISLVLSFYRPAGEENELCGQVATTPEDENEAQAGWIEGAAILFSVIIVVLVTAFNDWSKEKQFRGLQCRIEQEQKFSIIRNGQLIQLPVAEIVVGDIAQVKYGDLLPADGILIQGNDLKIDESSLTGESDHVKKSLDKDPMLLSGTHVMEGSGRMVVTAVGVNSQTGIILTLLGVNEDDEGEKKKKGKKQGVPENRNKAKTQDGVALEIQPLNSQEGIDNEEKDKKAVKVPKKEKSVLQGKLTRLAVQIGKAGLLMSALTVFILILYFVIDNFVINRRPWLPECTPIYIQYFVKFFIIGITVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTMNRMTVVQAYIGGIHYRQIPSPDVFLPKVLDLIVNGISINSAYTSKILPPEKEGGLPRQVGNKTECALLGFVTDLKQDYQAVRNEVPEEKLYKVYTFNSVRKSMSTVIRNPNGGFRMYSKGASEIILRKCNRILDRKGEAVPFKNKDRDDMVRTVIEPMACDGLRTICIAYRDFDDTEPSWDNENEILTELTCIAVVGIEDPVRPEVPDAIAKCKQAGITVRMVTGDNINTARAIATKCGILTPGDDFLCLEGKEFNRLIRNEKGEVEQEKLDKIWPKLRVLARSSPTDKHTLVKGIIDSTVGEHRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFTSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVIVAFTGACITQDSPLKAVQMLWVNLIMDTFASLALATEPPTESLLKRRPYGRNKPLISRTMMKNILGHAFYQLIVIFILVFAGEKFFDIDSGRKAPLHSPPSQHYTIVFNTFVLMQLFNEINSRKIHGEKNVFSGIYRNIIFCSVVLGTFICQIFIVEFGGKPFSCTSLSLSQWLWCLFIGIGELLWGQFISAIPTRSLKFLKEAGHGTTKEEITKDAEGLDEIDHAEMELRRGQILWFRGLNRIQTQIDVINTFQTGASFKGVLRRQNMGQHLDVKLVPSSSYVAVAPVKSSPTTSVPAVSSPPMGSE

Target ORF information:

RefSeq Version	NM_001365783.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ATPase plasma membrane Ca2+ transporting 4 (ATP2B4), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001365783.2

ORF Insert Sequence:

ATGACGAACC CATCAGACCG TGTCTTGCCT GCCAACTCGA TGGCCGAGAG CCGTGAAGGG 
GACTTTGGCT GCACAGTAAT GGAACTGAGG AAGCTCATGG AGCTGCGTTC AAGGGATGCA 
CTGACCCAGA TTAATGTCCA CTATGGAGGT GTACAGAATC TCTGCAGTAG ACTGAAAACC 
TCCCCTGTGG AAGGTCTGTC TGGGAACCCT GCAGATCTGG AGAAACGTAG GCAGGTGTTT 
GGACACAACG TGATCCCCCC CAAAAAGCCC AAGACTTTCT TAGAATTAGT GTGGGAAGCT 
CTTCAAGATG TCACGCTTAT CATCCTGGAG ATTGCAGCCA TCATCTCCCT GGTCCTGTCC 
TTTTATCGCC CTGCTGGTGA AGAAAATGAA CTGTGTGGTC AAGTCGCAAC TACCCCAGAA 
GATGAAAATG AGGCACAAGC TGGCTGGATT GAGGGGGCAG CCATCCTTTT CTCAGTGATC 
ATCGTGGTGT TAGTGACTGC CTTTAATGAT TGGAGCAAAG AGAAGCAATT CCGGGGGCTG 
CAGTGCCGCA TTGAACAGGA GCAAAAGTTC TCCATCATCC GAAACGGTCA ACTCATCCAG 
CTCCCTGTGG CTGAGATTGT GGTTGGTGAT ATTGCCCAAG TCAAATACGG TGATCTGCTG 
CCTGCAGATG GAATCCTGAT CCAAGGGAAT GATCTGAAGA TTGATGAGAG CTCTCTGACA 
GGGGAATCTG ACCATGTCAA GAAGTCCCTG GACAAAGACC CCATGTTGCT CTCAGGGACC 
CATGTCATGG AAGGTTCTGG CCGGATGGTG GTGACAGCTG TTGGTGTCAA CTCTCAGACT 
GGAATCATCC TTACTCTCTT GGGGGTCAAT GAGGATGACG AAGGGGAGAA AAAGAAGAAA 
GGTAAAAAAC AAGGAGTCCC TGAAAATCGC AACAAAGCAA AGACCCAAGA CGGAGTGGCC 
CTGGAAATCC AGCCACTCAA CAGCCAGGAG GGAATCGACA ATGAGGAAAA GGACAAGAAG 
GCAGTCAAGG TGCCTAAAAA GGAGAAGTCA GTGCTGCAGG GCAAGCTGAC TCGCCTGGCT 
GTTCAGATTG GGAAAGCCGG TCTGCTCATG TCTGCTCTCA CGGTTTTCAT CCTGATTCTA 
TACTTTGTGA TTGACAACTT TGTGATAAAT CGCAGACCAT GGCTCCCTGA GTGTACTCCC 
ATCTACATCC AGTACTTTGT CAAGTTCTTC ATCATCGGCA TCACTGTACT GGTGGTGGCT 
GTGCCAGAGG GGCTGCCTCT GGCTGTCACC ATCTCACTGG CCTACTCTGT GAAGAAAATG 
ATGAAAGACA ATAACCTAGT ACGGCACTTG GATGCTTGTG AGACCATGGG CAACGCCACC 
GCCATCTGCT CTGATAAGAC AGGCACGTTG ACCATGAACC GCATGACTGT GGTACAAGCT 
TATATTGGGG GCATCCATTA CCGTCAAATC CCAAGCCCTG ATGTCTTCCT GCCCAAAGTC 
CTGGACCTCA TTGTCAATGG CATTTCTATC AACAGTGCTT ATACCTCCAA GATTCTGCCT 
CCAGAGAAGG AGGGAGGCCT GCCTCGGCAG GTGGGCAACA AGACCGAGTG TGCTCTGCTA 
GGCTTTGTCA CAGATCTGAA GCAGGATTAT CAGGCTGTGC GTAATGAAGT GCCCGAGGAG 
AAGCTCTACA AGGTGTACAC CTTTAACTCA GTGCGCAAGT CAATGAGCAC CGTCATCAGG 
AATCCCAACG GTGGCTTCCG TATGTACAGC AAGGGCGCCT CTGAGATCAT CTTGCGCAAG 
TGTAATCGAA TCCTGGACCG GAAAGGGGAA GCAGTGCCAT TCAAGAATAA AGACAGAGAT 
GATATGGTAC GCACTGTCAT CGAGCCCATG GCCTGTGATG GACTCCGGAC TATCTGCATA 
GCTTACCGGG ACTTCGATGA CACAGAGCCC TCTTGGGACA ATGAGAATGA GATCCTCACC 
GAACTGACCT GTATCGCGGT GGTGGGCATT GAGGACCCTG TGCGCCCAGA GGTGCCAGAT 
GCTATTGCCA AATGCAAACA AGCTGGCATT ACTGTCAGAA TGGTGACAGG TGACAACATC 
AACACAGCCC GGGCCATTGC CACCAAATGT GGCATTCTGA CACCTGGGGA TGACTTCCTG 
TGCTTAGAAG GCAAAGAATT CAACCGGCTC ATCCGCAACG AGAAAGGCGA GGTAGAGCAA 
GAAAAGCTGG ACAAGATCTG GCCTAAGCTT CGGGTCCTGG CGCGATCTTC TCCCACTGAC 
AAGCACACCC TGGTGAAAGG CATAATTGAC AGCACTGTTG GGGAACACCG GCAGGTCGTG 
GCTGTCACTG GTGATGGCAC AAATGACGGG CCTGCTCTGA AGAAAGCGGA TGTTGGTTTT 
GCCATGGGCA TCGCAGGCAC AGATGTAGCA AAGGAGGCTT CAGACATCAT CCTAACAGAT 
GACAACTTCA CCAGCATTGT GAAGGCAGTG ATGTGGGGAC GAAATGTCTA TGACAGCATC 
TCCAAGTTCC TGCAGTTCCA GCTCACTGTC AATGTGGTGG CCGTGATTGT AGCCTTCACT 
GGAGCCTGTA TCACTCAGGA TTCCCCATTG AAAGCTGTGC AGATGTTGTG GGTTAATCTG 
ATCATGGACA CTTTTGCTTC ATTGGCCCTG GCCACAGAGC CCCCTACGGA ATCTCTGTTG 
AAGCGGCGCC CCTATGGCCG AAATAAGCCT CTGATCTCAC GCACTATGAT GAAGAACATC 
TTGGGCCATG CATTCTATCA GCTCATTGTC ATCTTTATCC TTGTCTTTGC GGGTGAGAAA 
TTCTTTGATA TTGATAGTGG GAGGAAGGCA CCTCTACATT CACCACCCAG CCAGCACTAT 
ACCATTGTTT TTAACACCTT CGTGCTGATG CAGCTCTTCA ATGAAATCAA CTCCCGAAAG 
ATCCATGGAG AGAAGAACGT CTTTTCAGGC ATCTACCGCA ACATTATCTT CTGCTCTGTA 
GTCTTGGGCA CATTCATCTG CCAGATTTTC ATCGTGGAAT TTGGGGGTAA ACCCTTCAGT 
TGTACAAGCC TCAGCCTGTC TCAGTGGCTG TGGTGTCTCT TCATTGGGAT TGGAGAACTT 
CTGTGGGGCC AGTTCATCTC CGCAATACCT ACCCGATCCC TGAAGTTCCT GAAGGAGGCT 
GGGCATGGCA CCACCAAAGA GGAGATCACC AAGGATGCCG AGGGACTGGA TGAGATTGAC 
CATGCTGAGA TGGAGCTGCG CCGAGGCCAG ATCCTCTGGT TCCGGGGCCT GAACCGTATC 
CAGACTCAGA TCGACGTAAT TAACACATTC CAGACGGGAG CCTCTTTTAA GGGAGTCCTA 
AGGCGACAGA ACATGGGTCA ACACCTTGAT GTAAAACTTG TTCCTAGTTC ATCCTATGTA 
GCAGTTGCAC CAGTCAAATC TTCCCCCACC ACTTCTGTTC CTGCTGTTTC ATCTCCTCCT 
ATGGGCAGTG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The serum protein renalase reduces injury in experimental pancreatitis.
The Journal of biological chemistry292(51)21047-21059(2017 Dec)
Kolodecik TR,Reed AM,Date K,Shugrue CA,Patel V,Chung SL,Desir GV,Gorelick FS

Sperm Na
Systems biology in reproductive medicine63(5)294-302(2017 Oct)
Lestari SW,Miati DN,Seoharso P,Sugiyanto R,Pujianto DA

Selective inhibition of plasma membrane calcium ATPase 4 improves angiogenesis and vascular reperfusion.
Journal of molecular and cellular cardiology10938-47(2017 Aug)
Kurusamy S,L?pez-Maderuelo D,Little R,Cadagan D,Savage AM,Ihugba JC,Baggott RR,Rowther FB,Mart?nez-Mart?nez S,Arco PG,Murcott C,Wang W,Francisco Nistal J,Oceandy D,Neyses L,Wilkinson RN,Cartwright EJ,Redondo JM,Armesilla AL

An erythroid-specific ATP2B4 enhancer mediates red blood cell hydration and malaria susceptibility.
The Journal of clinical investigation127(8)3065-3074(2017 Aug)
Lessard S,Gatof ES,Beaudoin M,Schupp PG,Sher F,Ali A,Prehar S,Kurita R,Nakamura Y,Baena E,Ledoux J,Oceandy D,Bauer DE,Lettre G

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ATP2B4 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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