ACADVL cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	ACADVL
Entrez Gene ID	282130
Full Name	acyl-CoA dehydrogenase very long chain
General protein information	Preferred Names acyl-CoA dehydrogenase very long chain Names very long-chain specific acyl-CoA dehydrogenase, mitochondrial VLCAD acyl-coenzyme A dehydrogenase, very long chain
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024979617.1	XP_024835385.1	very long-chain specific acyl-CoA dehydrogenase, mitochondrial isoform X1
NM_174494.2	NP_776919.1	very long-chain specific acyl-CoA dehydrogenase, mitochondrial precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ACADVL	XP_003315394.1
Drosophila melanogaster (fruit fly)	CG7461	NP_611409.2
Canis lupus familiaris (dog)	ACADVL	XP_546581.3
Mus musculus (house mouse)	Acadvl	NP_059062.1
Caenorhabditis elegans (roundworm)	acdh-12	NP_001022062.1
Danio rerio (zebrafish)	acadvl	NP_997776.1
Homo sapiens (human)	ACADVL	NP_000009.1
Bos taurus (cattle)	ACADVL	NP_776919.1
Rattus norvegicus (Norway rat)	Acadvl	NP_037023.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008769	XP_314893.4

Gene Ontology
Bibliography

Related articles in PubMed

Tetra-primer amplification refractory mutation system PCR (T-ARMS-PCR) rapidly identified a critical missense mutation (P236T) of bovine ACADVL gene affecting growth traits.
Zhang S, Dang Y, Zhang Q, Qin Q, Lei C, Chen H, Lan X
Gene559(2)184-8(2015 Apr)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Characterization of 954 bovine full-CDS cDNA sequences.
Harhay GP, Sonstegard TS, Keele JW, Heaton MP, Clawson ML, Snelling WM, Wiedmann RT, Van Tassell CP, Smith TP
BMC genomics6166(2005 Nov)

Analysis of bovine mammary gland EST and functional annotation of the Bos taurus gene index.
Sonstegard TS, Capuco AV, White J, Van Tassell CP, Connor EE, Cho J, Sultana R, Shade L, Wray JE, Wells KD, Quackenbush J
Mammalian genome : official journal of the International Mammalian Genome Society13(7)373-9(2002 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Bovine ACADVL gene had a significant effect on chest width (P<0.05), chest depth (P<0.05), and hip width (P<0.05) in the Qinchuan breed.
Title: Tetra-primer amplification refractory mutation system PCR (T-ARMS-PCR) rapidly identified a critical missense mutation (P236T) of bovine ACADVL gene affecting growth traits.

The following ACADVL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ACADVL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa41794	NM_174494.2 Latest version!	Bos taurus acyl-CoA dehydrogenase very long chain (ACADVL), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa225978	XM_024979617.1 Latest version!	Bos taurus acyl-CoA dehydrogenase very long chain (ACADVL), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ACADVL ( NM_174494.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776919.1 Bos taurus acyl-CoA dehydrogenase very long chain (ACADVL), mRNA.

Price & Availability↑

CloneID	OBa41794
Clone ID Related Accession (Same CDS sequence)	NM_174494.2
Accession Version	NM_174494.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Bos taurus(cattle)
Product	very long-chain specific acyl-CoA dehydrogenase, mitochondrial precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U30817.1. On or before Mar 29, 2005 this sequence version replaced XM_594343.1, NM_174494.1. ##Evidence-Data-START## Transcript exon combination :: U30817.1, BC103104.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGCAGGCGG CGCGGATGAC CGCGAGCTTG GGCCGTACTC TGCTGAGGCT GCGGGGCGTA 
AGCTCGTGGC CCGGTGAGCT CTTGGGGCAG CCCCGGCCCG GCCCTGCCCC GCGACCCTAT 
GCCAGTGGGG TCGCCCAGGC GGCTGTGGAC CAGTCTGATT CCCAGCCTTC TGAGGCTTCG 
ACCAGGGAAA AACGGGCCAA CTCGGTATCT AAGTCCTTTG CTGTGGGCAC GTTCAAGGGC 
CAGCTCACCA CCGATCAGGT GTTTCCATAC CCGTCTGTGC TCAACGAGGA CCAAACACAG 
TTTCTCAAAG AGCTGGTGGG GCCCGTGACC CGATTCTTTG AGGAGGTGAA CGATGCTGCC 
AAGAATGACA TGCTGGAAAG AGTGGAGGAG ACCACCATGC AAGGTCTCAA GGAGTTGGGG 
GCCTTTGGTC TGCAAGTACC CAATGAACTG GGTGGCGTGG GCCTCTGCAA CACCCAGTAT 
GCCCGATTGG TGGAGATCGT GGGCATGTAT GACCTTGGCG TGGGCATCGT CCTGGGGGCC 
CATCAGAGCA TCGGTTTCAA AGGCATCCTG CTCTTCGGCA CAAAGGCCCA GAAAGAAAAA 
TATCTCCCCA AACTGGCATC TGGGGAGACT ATAGCTGCTT TCTGTCTAAC GGAGCCCTCC 
AGTGGATCAG ATGCAGCGTC CATCCGATCC TCAGCTGTGC CCAGCCCCTG TGGAAAATAC 
TATACCCTCA ACGGAAGCAA GATTTGGATC AGTAACGGGG GCCTGGCAGA CATCTTCACG 
GTCTTTGCCA AGACACCGGT TACAGACACA GCTACGGGCG CTGTGAAGGA GAAGATCACA 
GCTTTTGTGG TGGAGAGGAG CTTTGGCGGC GTCACCCATG GGCCCCCTGA GAAGAAGATG 
GGCATCAAAG CCTCAAACAC AGCAGAGGTG TACTTTGACG GAGTACGGGT GCCAGCAGAG 
AACGTACTGG GGGAGGTAGG GGGCGGCTTC AAGGTCGCCA TGCATATTCT CAACAATGGA 
AGGTTTGGCA TGGCTGCAGC TCTGGCAGGC ACCATGAAAG GCATCATTGC TAAGGCGGTG 
GATCATGCTG CTAACCGTAC CCAGTTTGGG GAGAAAATTC ACAACTTTGG GCTGATCCAG 
GAGAAGCTGG CCCGGATGGC TATGCTGCAG TATGTGACTG AGTCCATGGC GTACATGGTG 
AGTGCCAACA TGGACCAGGG ATCCACGGAC TTCCAGATAG AGGCCGCCAT CAGCAAAATC 
TTTGGCTCGG AGGCAGCCTG GAAAGTGACA GATGAGTGCA TCCAAATCAT GGGGGGCATG 
GGCTTCATGA AGGAGCCTGG GGTAGAGCGT GTGCTCCGAG ATCTTCGCAT CTTCCGGATC 
TTCGAGGGGA CAAATGACAT TCTCCGGCTG TTTGTGGCTC TGCAGGGCTG CATGGACAAA 
GGAAAGGAAC TCTCTGGGCT TGGCAATGCT CTAAAGAACC CTTTTGGGAA TGCTGGCCTC 
CTGCTAGGAG AGGCAGGCAA ACAGCTGAGG CGGCGGGCAG GGCTGGGCAG TGGCCTGAGT 
CTCAGTGGCA TCGTCCACCA GGAACTGAGT CGGAGTGGTG AGCTGGCGGT GCAGGCTCTG 
GAGCAGTTTG CCACCGTGGT GGAGGCCAAG CTGATAAAGC ACAAGAAGGA TATCATCAAT 
GAACAGTTTC TGCTGCAGCG TCTGGCGGAC AGTGCCATTG ACCTCTATGC CATGGTGGTG 
GTTCTGTCCA GGGCCTCAAG ATCCCTGAGT GAAGGCCACC CCACAGCCCA GCATGAGAAA 
ATGCTCTGTG ACAGCTGGTG TATCGAGGCT GCAGCCCGGA TCCGGGAGAA CATGACTGCT 
CTGCAGTCTG ACCCCCAGCA GCAGGAGCTC TTTCGTAACT TCAAAAGCAT CTCCAAGGCG 
CTGGTGGAGC GGGGCGGCGT GGTCACCAGC AATCCCCTTG GTTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776919.1
CDS	7..1974
Translation	MQAARMTASLGRTLLRLRGVSSWPGELLGQPRPGPAPRPYASGVAQAAVDQSDSQPSEASTREKRANSVSKSFAVGTFKGQLTTDQVFPYPSVLNEDQTQFLKELVGPVTRFFEEVNDAAKNDMLERVEETTMQGLKELGAFGLQVPNELGGVGLCNTQYARLVEIVGMYDLGVGIVLGAHQSIGFKGILLFGTKAQKEKYLPKLASGETIAAFCLTEPSSGSDAASIRSSAVPSPCGKYYTLNGSKIWISNGGLADIFTVFAKTPVTDTATGAVKEKITAFVVERSFGGVTHGPPEKKMGIKASNTAEVYFDGVRVPAENVLGEVGGGFKVAMHILNNGRFGMAAALAGTMKGIIAKAVDHAANRTQFGEKIHNFGLIQEKLARMAMLQYVTESMAYMVSANMDQGSTDFQIEAAISKIFGSEAAWKVTDECIQIMGGMGFMKEPGVERVLRDLRIFRIFEGTNDILRLFVALQGCMDKGKELSGLGNALKNPFGNAGLLLGEAGKQLRRRAGLGSGLSLSGIVHQELSRSGELAVQALEQFATVVEAKLIKHKKDIINEQFLLQRLADSAIDLYAMVVVLSRASRSLSEGHPTAQHEKMLCDSWCIEAAARIRENMTALQSDPQQQELFRNFKSISKALVERGGVVTSNPLGF

Target ORF information:

RefSeq Version	NM_174494.2
Organism	Bos taurus(cattle)
Definition	Bos taurus acyl-CoA dehydrogenase very long chain (ACADVL), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174494.2

ORF Insert Sequence:

ATGCAGGCGG CGCGGATGAC CGCGAGCTTG GGCCGTACTC TGCTGAGGCT GCGGGGCGTA 
AGCTCGTGGC CCGGTGAGCT CTTGGGGCAG CCCCGGCCCG GCCCTGCCCC GCGACCCTAT 
GCCAGTGGGG TCGCCCAGGC GGCTGTGGAC CAGTCTGATT CCCAGCCTTC TGAGGCTTCG 
ACCAGGGAAA AACGGGCCAA CTCGGTATCT AAGTCCTTTG CTGTGGGCAC GTTCAAGGGC 
CAGCTCACCA CCGATCAGGT GTTTCCATAC CCGTCTGTGC TCAACGAGGA CCAAACACAG 
TTTCTCAAAG AGCTGGTGGG GCCCGTGACC CGATTCTTTG AGGAGGTGAA CGATGCTGCC 
AAGAATGACA TGCTGGAAAG AGTGGAGGAG ACCACCATGC AAGGTCTCAA GGAGTTGGGG 
GCCTTTGGTC TGCAAGTACC CAATGAACTG GGTGGCGTGG GCCTCTGCAA CACCCAGTAT 
GCCCGATTGG TGGAGATCGT GGGCATGTAT GACCTTGGCG TGGGCATCGT CCTGGGGGCC 
CATCAGAGCA TCGGTTTCAA AGGCATCCTG CTCTTCGGCA CAAAGGCCCA GAAAGAAAAA 
TATCTCCCCA AACTGGCATC TGGGGAGACT ATAGCTGCTT TCTGTCTAAC GGAGCCCTCC 
AGTGGATCAG ATGCAGCGTC CATCCGATCC TCAGCTGTGC CCAGCCCCTG TGGAAAATAC 
TATACCCTCA ACGGAAGCAA GATTTGGATC AGTAACGGGG GCCTGGCAGA CATCTTCACG 
GTCTTTGCCA AGACACCGGT TACAGACACA GCTACGGGCG CTGTGAAGGA GAAGATCACA 
GCTTTTGTGG TGGAGAGGAG CTTTGGCGGC GTCACCCATG GGCCCCCTGA GAAGAAGATG 
GGCATCAAAG CCTCAAACAC AGCAGAGGTG TACTTTGACG GAGTACGGGT GCCAGCAGAG 
AACGTACTGG GGGAGGTAGG GGGCGGCTTC AAGGTCGCCA TGCATATTCT CAACAATGGA 
AGGTTTGGCA TGGCTGCAGC TCTGGCAGGC ACCATGAAAG GCATCATTGC TAAGGCGGTG 
GATCATGCTG CTAACCGTAC CCAGTTTGGG GAGAAAATTC ACAACTTTGG GCTGATCCAG 
GAGAAGCTGG CCCGGATGGC TATGCTGCAG TATGTGACTG AGTCCATGGC GTACATGGTG 
AGTGCCAACA TGGACCAGGG ATCCACGGAC TTCCAGATAG AGGCCGCCAT CAGCAAAATC 
TTTGGCTCGG AGGCAGCCTG GAAAGTGACA GATGAGTGCA TCCAAATCAT GGGGGGCATG 
GGCTTCATGA AGGAGCCTGG GGTAGAGCGT GTGCTCCGAG ATCTTCGCAT CTTCCGGATC 
TTCGAGGGGA CAAATGACAT TCTCCGGCTG TTTGTGGCTC TGCAGGGCTG CATGGACAAA 
GGAAAGGAAC TCTCTGGGCT TGGCAATGCT CTAAAGAACC CTTTTGGGAA TGCTGGCCTC 
CTGCTAGGAG AGGCAGGCAA ACAGCTGAGG CGGCGGGCAG GGCTGGGCAG TGGCCTGAGT 
CTCAGTGGCA TCGTCCACCA GGAACTGAGT CGGAGTGGTG AGCTGGCGGT GCAGGCTCTG 
GAGCAGTTTG CCACCGTGGT GGAGGCCAAG CTGATAAAGC ACAAGAAGGA TATCATCAAT 
GAACAGTTTC TGCTGCAGCG TCTGGCGGAC AGTGCCATTG ACCTCTATGC CATGGTGGTG 
GTTCTGTCCA GGGCCTCAAG ATCCCTGAGT GAAGGCCACC CCACAGCCCA GCATGAGAAA 
ATGCTCTGTG ACAGCTGGTG TATCGAGGCT GCAGCCCGGA TCCGGGAGAA CATGACTGCT 
CTGCAGTCTG ACCCCCAGCA GCAGGAGCTC TTTCGTAACT TCAAAAGCAT CTCCAAGGCG 
CTGGTGGAGC GGGGCGGCGT GGTCACCAGC AATCCCCTTG GTTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACADVL ( XM_024979617.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024835385.1 Bos taurus acyl-CoA dehydrogenase very long chain (ACADVL), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa225978
Clone ID Related Accession (Same CDS sequence)	XM_024979617.1
Accession Version	XM_024979617.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1905bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	very long-chain specific acyl-CoA dehydrogenase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGGCAG CGAGAATGGC TCAGAGTACG GGGCGGCAGC TGCTGAGGCT GCGGGGCGTA 
AGCTCGTGGC CCGGTGAGCT CTTGGGGCAG CCCCGGCCCG GCCCTGCCCG GCGACCCTAT 
GCCAGTGGGG TCGCCCAGGC GGCTGTGGAC CAGTCTGATT CCCAGCCTTC TGAGGCTTCG 
ACCAGGGAAA AACGGGCCAA CTCGGTATCT AAGTCCTTTG CTGTGGGCAC GTTCAAGGGC 
CAGCTCACCA CCGATCAGGT GTTTCCATAC CCGTCTGTGC TCAACGAGGA CCAAACACAG 
TTTCTCAAAG AGCTGGTGGG GCCCGTGACC CGATTCTTTG AGGAGGTGAA CGATGCTGCC 
AAGAATGACA TGCTGGAAAG AGTGGAGGAG ACCACCATGC AAGGTCTCAA GGAGTTGGGG 
GCCTTTGGTC TGCAAGTACC CAATGAACTG GGTGGCGTGG GCCTCTGCAA CACCCAGTAT 
GCCCGATTGG TGGAGATCGT GGGCATGTAT GACCTTGGCG TGGGCATCGT CCTGGGGGCC 
CATCAGAGCA TCGGTTTCAA AGGCATCCTG CTCTTCGGCA CAAAGGCCCA GAAAGAAAAA 
TATCTCCCCA AACTGGCATC TGGGGAGACT ATAGCTGCTT TCTGTCTAAC GGAGCCCTCC 
AGTGGATCAG ATGCAGCGTC CATCCGATCC TCAGCTGTGC CCAGCCCCTG TGGAAAATAC 
TATACCCTCA ACGGAAGCAA GATTTGGATC AGTAACGGGG GCCTGGCAGA CATCTTCACG 
GTCTTTGCCA AGACACCGGT TACAGACACA GCTACGGGCG CTGTGAAGGA GAAGATCACA 
GCTTTTGTGG TGGAGAGGAG CTTTGGCGGC GTCACCCATG GGCCCCCTGA GAAGAAGATG 
GGCATCAAAG CCTCAAACAC AGCAGAGGTG TACTTTGACG GAGTACGGGT GCCAGCAGAG 
AACGTACTGG GGGAGGTAGG GGGCGGCTTC AAGGTCGCCA TGCATATTCT CAACAATGGA 
AGGTTTGGCA TGGCTGCAGC TCTGGCAGGC ACCATGAAAG GCATCATTGC TAAGGCGGTG 
GATCATGCTG CTAACCGTAC CCAGTTTGGG GAGAAAATTC ACAACTTTGG GCTGATCCAG 
GAGAAGCTGG CCCGGATGGC TATGCTGCAG TATGTGACTG AGTCCATGGC GTACATGGTG 
AGTGCCAACA TGGACCAGGG ATCCACGGAC TTCCAGATAG AGGCCGCCAT CAGCAAAATC 
TTTGGCTCGG AGCCTGGGGT AGAGCGTGTG CTCCGAGATC TTCGCATCTT CCGGATCTTC 
GAGGGGACAA ATGACATTCT CCGGCTGTTT GTGGCTCTGC AGGGCTGCAT GGACAAAGGA 
AAGGAACTCT CTGGGCTTGG CAATGCTCTA AAGAACCCTT TTGGGAATGC TGGCCTCCTG 
CTAGGAGAGG CAGGCAAACA GCTGAGGCGG CGGGCAGGGC TGGGCAGTGG CCTGAGTCTC 
AGTGGCATCG TCCACCAGGA ACTGAGTCGG AGTGGTGAGC TGGCGGTGCA GGCTCTGGAG 
CAGTTTGCCA CCGTGGTGGA GGCCAAGCTG ATAAAGCACA AGAAGGATAT CATCAATGAA 
CAGTTTCTGC TGCAGCGTCT GGCGGACAGT GCCATTGACC TCTATGCCAT GGTGGTGGTT 
CTGTCCAGGG CCTCAAGATC CCTGAGTGAA GGCCACCCCA CAGCCCAGCA TGAGAAAATG 
CTCTGTGACA GCTGGTGTAT CGAGGCTGCA GCCCGGATCC GGGAGAACAT GACTGCTCTG 
CAGTCTGACC CCCAGCAGCA GGAGCTCTTT CGTAACTTCA AAAGCATCTC CAAGGCCCTG 
GTGGAGCGGG GCGGCGTGGT CACCAGCAAT CCCCTTGGTT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024835385.1
CDS	111..2015
Translation	MRAARMAQSTGRQLLRLRGVSSWPGELLGQPRPGPARRPYASGVAQAAVDQSDSQPSEASTREKRANSVSKSFAVGTFKGQLTTDQVFPYPSVLNEDQTQFLKELVGPVTRFFEEVNDAAKNDMLERVEETTMQGLKELGAFGLQVPNELGGVGLCNTQYARLVEIVGMYDLGVGIVLGAHQSIGFKGILLFGTKAQKEKYLPKLASGETIAAFCLTEPSSGSDAASIRSSAVPSPCGKYYTLNGSKIWISNGGLADIFTVFAKTPVTDTATGAVKEKITAFVVERSFGGVTHGPPEKKMGIKASNTAEVYFDGVRVPAENVLGEVGGGFKVAMHILNNGRFGMAAALAGTMKGIIAKAVDHAANRTQFGEKIHNFGLIQEKLARMAMLQYVTESMAYMVSANMDQGSTDFQIEAAISKIFGSEPGVERVLRDLRIFRIFEGTNDILRLFVALQGCMDKGKELSGLGNALKNPFGNAGLLLGEAGKQLRRRAGLGSGLSLSGIVHQELSRSGELAVQALEQFATVVEAKLIKHKKDIINEQFLLQRLADSAIDLYAMVVVLSRASRSLSEGHPTAQHEKMLCDSWCIEAAARIRENMTALQSDPQQQELFRNFKSISKALVERGGVVTSNPLGF

Target ORF information:

RefSeq Version	XM_024979617.1
Organism	Bos taurus(cattle)
Definition	Bos taurus acyl-CoA dehydrogenase very long chain (ACADVL), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024979617.1

ORF Insert Sequence:

ATGCGGGCAG CGAGAATGGC TCAGAGTACG GGGCGGCAGC TGCTGAGGCT GCGGGGCGTA 
AGCTCGTGGC CCGGTGAGCT CTTGGGGCAG CCCCGGCCCG GCCCTGCCCG GCGACCCTAT 
GCCAGTGGGG TCGCCCAGGC GGCTGTGGAC CAGTCTGATT CCCAGCCTTC TGAGGCTTCG 
ACCAGGGAAA AACGGGCCAA CTCGGTATCT AAGTCCTTTG CTGTGGGCAC GTTCAAGGGC 
CAGCTCACCA CCGATCAGGT GTTTCCATAC CCGTCTGTGC TCAACGAGGA CCAAACACAG 
TTTCTCAAAG AGCTGGTGGG GCCCGTGACC CGATTCTTTG AGGAGGTGAA CGATGCTGCC 
AAGAATGACA TGCTGGAAAG AGTGGAGGAG ACCACCATGC AAGGTCTCAA GGAGTTGGGG 
GCCTTTGGTC TGCAAGTACC CAATGAACTG GGTGGCGTGG GCCTCTGCAA CACCCAGTAT 
GCCCGATTGG TGGAGATCGT GGGCATGTAT GACCTTGGCG TGGGCATCGT CCTGGGGGCC 
CATCAGAGCA TCGGTTTCAA AGGCATCCTG CTCTTCGGCA CAAAGGCCCA GAAAGAAAAA 
TATCTCCCCA AACTGGCATC TGGGGAGACT ATAGCTGCTT TCTGTCTAAC GGAGCCCTCC 
AGTGGATCAG ATGCAGCGTC CATCCGATCC TCAGCTGTGC CCAGCCCCTG TGGAAAATAC 
TATACCCTCA ACGGAAGCAA GATTTGGATC AGTAACGGGG GCCTGGCAGA CATCTTCACG 
GTCTTTGCCA AGACACCGGT TACAGACACA GCTACGGGCG CTGTGAAGGA GAAGATCACA 
GCTTTTGTGG TGGAGAGGAG CTTTGGCGGC GTCACCCATG GGCCCCCTGA GAAGAAGATG 
GGCATCAAAG CCTCAAACAC AGCAGAGGTG TACTTTGACG GAGTACGGGT GCCAGCAGAG 
AACGTACTGG GGGAGGTAGG GGGCGGCTTC AAGGTCGCCA TGCATATTCT CAACAATGGA 
AGGTTTGGCA TGGCTGCAGC TCTGGCAGGC ACCATGAAAG GCATCATTGC TAAGGCGGTG 
GATCATGCTG CTAACCGTAC CCAGTTTGGG GAGAAAATTC ACAACTTTGG GCTGATCCAG 
GAGAAGCTGG CCCGGATGGC TATGCTGCAG TATGTGACTG AGTCCATGGC GTACATGGTG 
AGTGCCAACA TGGACCAGGG ATCCACGGAC TTCCAGATAG AGGCCGCCAT CAGCAAAATC 
TTTGGCTCGG AGCCTGGGGT AGAGCGTGTG CTCCGAGATC TTCGCATCTT CCGGATCTTC 
GAGGGGACAA ATGACATTCT CCGGCTGTTT GTGGCTCTGC AGGGCTGCAT GGACAAAGGA 
AAGGAACTCT CTGGGCTTGG CAATGCTCTA AAGAACCCTT TTGGGAATGC TGGCCTCCTG 
CTAGGAGAGG CAGGCAAACA GCTGAGGCGG CGGGCAGGGC TGGGCAGTGG CCTGAGTCTC 
AGTGGCATCG TCCACCAGGA ACTGAGTCGG AGTGGTGAGC TGGCGGTGCA GGCTCTGGAG 
CAGTTTGCCA CCGTGGTGGA GGCCAAGCTG ATAAAGCACA AGAAGGATAT CATCAATGAA 
CAGTTTCTGC TGCAGCGTCT GGCGGACAGT GCCATTGACC TCTATGCCAT GGTGGTGGTT 
CTGTCCAGGG CCTCAAGATC CCTGAGTGAA GGCCACCCCA CAGCCCAGCA TGAGAAAATG 
CTCTGTGACA GCTGGTGTAT CGAGGCTGCA GCCCGGATCC GGGAGAACAT GACTGCTCTG 
CAGTCTGACC CCCAGCAGCA GGAGCTCTTT CGTAACTTCA AAAGCATCTC CAAGGCCCTG 
GTGGAGCGGG GCGGCGTGGT CACCAGCAAT CCCCTTGGTT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Tetra-primer amplification refractory mutation system PCR (T-ARMS-PCR) rapidly identified a critical missense mutation (P236T) of bovine ACADVL gene affecting growth traits.
Gene559(2)184-8(2015 Apr)
Zhang S,Dang Y,Zhang Q,Qin Q,Lei C,Chen H,Lan X

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Characterization of 954 bovine full-CDS cDNA sequences.
BMC genomics6166(2005 Nov)
Harhay GP,Sonstegard TS,Keele JW,Heaton MP,Clawson ML,Snelling WM,Wiedmann RT,Van Tassell CP,Smith TP

Analysis of bovine mammary gland EST and functional annotation of the Bos taurus gene index.
Mammalian genome : official journal of the International Mammalian Genome Society13(7)373-9(2002 Jul)
Sonstegard TS,Capuco AV,White J,Van Tassell CP,Connor EE,Cho J,Sultana R,Shade L,Wray JE,Wells KD,Quackenbush J

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ACADVL cDNA ORF clone, Bos taurus(cattle)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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