Acadvl cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Acadvl
Entrez Gene ID	11370
Full Name	acyl-Coenzyme A dehydrogenase, very long chain
Synonyms	vlcad
General protein information	Preferred Names acyl-Coenzyme A dehydrogenase, very long chain Names very long-chain specific acyl-CoA dehydrogenase, mitochondrial MVLCAD
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11 B3\|11 42.96 cM
Summary	This gene encodes a homodimeric mitochondrial flavoprotein and is a member of the acyl-CoA dehydrogenase family. Members of this family catalyze the first step of fatty acid beta-oxidation, forming a C2-C3 trans-double bond in a FAD-dependent reaction. As beta-oxidation cycles through its four steps, each member of the acyl-CoA dehydrogenase family works at an optimum fatty acid chain-length. This enzyme has its optimum length between C16- and C20-acylCoA and localizes to the inner mitochondrial membrane (unlike related acyl-CoA dehydrogenases). In mice, deficiency of this gene can cause ventricular arrhythmias as well as fasting and cold intolerance. [provided by RefSeq, Nov 2012].

mRNA and Protein(s)

mRNA	Protein	Name
NM_017366.3	NP_059062.1	very long-chain specific acyl-CoA dehydrogenase, mitochondrial precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ACADVL	XP_003315394.1
Drosophila melanogaster (fruit fly)	CG7461	NP_611409.2
Canis lupus familiaris (dog)	ACADVL	XP_546581.3
Mus musculus (house mouse)	Acadvl	NP_059062.1
Caenorhabditis elegans (roundworm)	acdh-12	NP_001022062.1
Danio rerio (zebrafish)	acadvl	NP_997776.1
Homo sapiens (human)	ACADVL	NP_000009.1
Bos taurus (cattle)	ACADVL	NP_776919.1
Rattus norvegicus (Norway rat)	Acadvl	NP_037023.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008769	XP_314893.4

Gene Ontology
Bibliography

Related articles in PubMed

Gestational, pathologic and biochemical differences between very long-chain acyl-CoA dehydrogenase deficiency and long-chain acyl-CoA dehydrogenase deficiency in the mouse.
Cox KB, Hamm DA, Millington DS, Matern D, Vockley J, Rinaldo P, Pinkert CA, Rhead WJ, Lindsey JR, Wood PA
Human molecular genetics10(19)2069-77(2001 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Triheptanoin was not able to prevent the development of systolic dysfunction in VLCAD(-/-) mice despite an upregulation of cardiac glucose oxidation. Strikingly, the anaplerotic effects of triheptanoin were restricted to the liver.
Title: Triheptanoin: long-term effects in the very long-chain acyl-CoA dehydrogenase-deficient mouse.

observed strong upregulation of peroxisomal beta-oxidation in VLCAD(-/-) mice
Title: De?novo fatty acid biosynthesis and elongation in very long-chain acyl-CoA dehydrogenase-deficient mice supplemented with odd or even medium-chain fatty acids.

SIRT3 and SIRT5 regulate the enzyme activity and cardiolipin binding of very long-chain acyl-CoA dehydrogenase
Title: SIRT3 and SIRT5 regulate the enzyme activity and cardiolipin binding of very long-chain acyl-CoA dehydrogenase.

We demonstrate here that both dietary interventions with respect to the fat content of the diet reverse endogenous compensatory mechanisms in muscle that have evolved in VLCAD(-/-) mice resulting in pronounced energy deficiency
Title: Long-term dietary effects on substrate selection and muscle fiber type in very-long-chain acyl-CoA dehydrogenase deficient (VLCAD(-/-)) mice.

Studies conducted with permeabilized mitochondria and different chain length acyl-CoA derivatives suggest that VLCAD is also a source of reactive oxygen species production in mitochondria of high fat diet animals.
Title: Diet-sensitive sources of reactive oxygen species in liver mitochondria: role of very long chain acyl-CoA dehydrogenases.

The following Acadvl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Acadvl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu16244	NM_017366.3 Latest version!	Mus musculus acyl-Coenzyme A dehydrogenase, very long chain (Acadvl), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$384.30 $549.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Acadvl ( NM_017366.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_059062.1 Mus musculus acyl-Coenzyme A dehydrogenase, very long chain (Acadvl), mRNA.

Price & Availability↑

CloneID	OMu16244
Clone ID Related Accession (Same CDS sequence)	NM_017366.3
Accession Version	NM_017366.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	very long-chain specific acyl-CoA dehydrogenase, mitochondrial precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AU067294.1, AK076037.1 and AI843782.1. On Nov 29, 2012 this sequence version replaced NM_017366.2. Sequence Note: The transit peptide annotated at aa 1-41 is inferred by similarity to the cleavage site in the orthologous rat protein as described in PMID: 8034667. The software program TargetP1.1 predicts that the transit peptide in mouse is aa 1-27. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC026559.1, AK076037.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164135 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCAGTCGG CTCGGATGAC CCCGAGTGTG GGGCGACAGC TGCTGCGGCT GGGGGCCCGA 
AGCTCGCGAT CTACTACTGT GCTTCAGGGA CAACCCCGGC CTATCTCTGC CCAGCGACTT 
TATGCCAGGG AGGCCACTCA GGCAGTTCTG GACAAGCCAG AAACCCTCTC CTCTGATGCT 
TCCACCAGAG AAAAACCAGC CAGGGCAGAA TCGAAGTCCT TTGCTGTGGG GATGTTCAAA 
GGCCAGCTTA CCATTGATCA GGTGTTCCCA TACCCATCTG TGCTCAGTGA AGAACAGGCA 
CAATTTCTCA AAGAGCTGGT GGGACCAGTG GCCCGGTTCT TTGAGGAAGT GAATGACCCT 
GCCAAGAACG ACGCCTTGGA GAAGGTGGAG GACGACACTT TGCAGGGACT CAAGGAACTG 
GGGGCATTTG GCCTGCAAGT ACCCAGCGAG CTGGGTGGTT TGGGCCTCTC TAATACCCAG 
TATGCTCGCT TGGCAGAGAT TGTGGGCATG CATGACCTTG GTGTTAGCGT TACCCTGGGA 
GCTCATCAGA GCATCGGTTT CAAAGGCATC TTGCTCTATG GCACAAAGGC CCAGAGAGAA 
AAATACCTTC CCAGAGTGGC ATCTGGGCAG GCTTTGGCTG CTTTCTGCCT AACAGAGCCC 
TCGAGTGGAT CCGATGTAGC CTCCATCCGA AGCTCAGCCA TACCCAGCCC CTGTGGAAAA 
TATTACACTC TCAATGGGAG CAAGATCTGG ATCAGTAATG GGGGCCTGGC TGACATTTTC 
ACTGTCTTTG CCAAGACGCC AATTAAAGAT GCAGCCACGG GGGCCGTGAA AGAGAAGATC 
ACAGCTTTTG TAGTGGAAAG GAGCTTCGGA GGGGTTACCC ATGGGCTCCC TGAAAAGAAG 
ATGGGCATCA AAGCATCTAA CACGTCAGAG GTGTACTTTG ATGGAGTGAA GGTGCCATCA 
GAGAATGTGC TAGGAGAGGT GGGAGATGGC TTCAAGGTTG CTGTCAACAT CCTCAACAAC 
GGAAGATTCG GGATGGCTGC AACCCTCGCA GGCACCATGA AATCCCTCAT TGCCAAGGCG 
GTTGATCATG CTACTAATCG TACCCAGTTT GGGGACAAAA TTCACAACTT TGGGGTGATC 
CAGGAAAAGC TGGCTCGGAT GGCTATTCTG CAGTATGTGA CTGAGTCCAT GGCTTACATG 
CTGAGTGCCA ACATGGACCA GGGATTCAAA GACTTCCAGA TAGAAGCCGC CATCAGCAAA 
ATCTTTTGCT CGGAGGCGGC CTGGAAAGTG GCAGATGAGT GCATCCAAAT AATGGGGGGC 
ATGGGCTTCA TGAAGGAACC AGGGGTGGAG CGTGTGCTCC GAGATATTCG AATCTTCCGG 
ATCTTTGAAG GGGCAAATGA CATTCTTCGA CTGTTTGTGG CTCTGCAAGG CTGTATGGAC 
AAAGGAAAGG AACTCACTGG GCTGGGCAAT GCCCTGAAGA ATCCTTTTGG AAACGTTGGC 
CTCCTGATGG GAGAAGCAGG CAAACAGCTG AGACGGAGGA CAGGAATCGG CAGTGGTTTG 
AGTCTGTCAG GGATTGTCCA CCCAGAGTTA AGTCGCAGTG GTGAACTGGC AGTGCAGGCT 
CTGGATCAAT TTGCCACCGT GGTGGAGGCC AAGCTGGTGA AACACAAGAA AGGGATTGTC 
AACGAGCAGT TCCTGCTGCA GCGACTGGCG GACGGCGCCA TTGACCTCTA TGCCATGGTG 
GTGGTTCTGT CCAGAGCCTC AAGGTCCCTG AGTGAGGGCT ACCCGACGGC ACAGCATGAG 
AAAATGCTCT GTGATAGCTG GTGCATTGAG GCTGCAACTC GGATCCGAGA AAACATGGCC 
AGTCTGCAGT CCAGCCCTCA GCATCAGGAG CTCTTCCGGA ACTTCAGAAG CATCTCCAAG 
GCCATGGTGG AGAATGGTGG CCTGGTCACC GGTAACCCCC TGGGAATCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_059062.1
CDS	105..2075
Translation	MQSARMTPSVGRQLLRLGARSSRSTTVLQGQPRPISAQRLYAREATQAVLDKPETLSSDASTREKPARAESKSFAVGMFKGQLTIDQVFPYPSVLSEEQAQFLKELVGPVARFFEEVNDPAKNDALEKVEDDTLQGLKELGAFGLQVPSELGGLGLSNTQYARLAEIVGMHDLGVSVTLGAHQSIGFKGILLYGTKAQREKYLPRVASGQALAAFCLTEPSSGSDVASIRSSAIPSPCGKYYTLNGSKIWISNGGLADIFTVFAKTPIKDAATGAVKEKITAFVVERSFGGVTHGLPEKKMGIKASNTSEVYFDGVKVPSENVLGEVGDGFKVAVNILNNGRFGMAATLAGTMKSLIAKAVDHATNRTQFGDKIHNFGVIQEKLARMAILQYVTESMAYMLSANMDQGFKDFQIEAAISKIFCSEAAWKVADECIQIMGGMGFMKEPGVERVLRDIRIFRIFEGANDILRLFVALQGCMDKGKELTGLGNALKNPFGNVGLLMGEAGKQLRRRTGIGSGLSLSGIVHPELSRSGELAVQALDQFATVVEAKLVKHKKGIVNEQFLLQRLADGAIDLYAMVVVLSRASRSLSEGYPTAQHEKMLCDSWCIEAATRIRENMASLQSSPQHQELFRNFRSISKAMVENGGLVTGNPLGI

Target ORF information:

RefSeq Version	NM_017366.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus acyl-Coenzyme A dehydrogenase, very long chain (Acadvl), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017366.3

ORF Insert Sequence:

ATGCAGTCGG CTCGGATGAC CCCGAGTGTG GGGCGACAGC TGCTGCGGCT GGGGGCCCGA 
AGCTCGCGAT CTACTACTGT GCTTCAGGGA CAACCCCGGC CTATCTCTGC CCAGCGACTT 
TATGCCAGGG AGGCCACTCA GGCAGTTCTG GACAAGCCAG AAACCCTCTC CTCTGATGCT 
TCCACCAGAG AAAAACCAGC CAGGGCAGAA TCGAAGTCCT TTGCTGTGGG GATGTTCAAA 
GGCCAGCTTA CCATTGATCA GGTGTTCCCA TACCCATCTG TGCTCAGTGA AGAACAGGCA 
CAATTTCTCA AAGAGCTGGT GGGACCAGTG GCCCGGTTCT TTGAGGAAGT GAATGACCCT 
GCCAAGAACG ACGCCTTGGA GAAGGTGGAG GACGACACTT TGCAGGGACT CAAGGAACTG 
GGGGCATTTG GCCTGCAAGT ACCCAGCGAG CTGGGTGGTT TGGGCCTCTC TAATACCCAG 
TATGCTCGCT TGGCAGAGAT TGTGGGCATG CATGACCTTG GTGTTAGCGT TACCCTGGGA 
GCTCATCAGA GCATCGGTTT CAAAGGCATC TTGCTCTATG GCACAAAGGC CCAGAGAGAA 
AAATACCTTC CCAGAGTGGC ATCTGGGCAG GCTTTGGCTG CTTTCTGCCT AACAGAGCCC 
TCGAGTGGAT CCGATGTAGC CTCCATCCGA AGCTCAGCCA TACCCAGCCC CTGTGGAAAA 
TATTACACTC TCAATGGGAG CAAGATCTGG ATCAGTAATG GGGGCCTGGC TGACATTTTC 
ACTGTCTTTG CCAAGACGCC AATTAAAGAT GCAGCCACGG GGGCCGTGAA AGAGAAGATC 
ACAGCTTTTG TAGTGGAAAG GAGCTTCGGA GGGGTTACCC ATGGGCTCCC TGAAAAGAAG 
ATGGGCATCA AAGCATCTAA CACGTCAGAG GTGTACTTTG ATGGAGTGAA GGTGCCATCA 
GAGAATGTGC TAGGAGAGGT GGGAGATGGC TTCAAGGTTG CTGTCAACAT CCTCAACAAC 
GGAAGATTCG GGATGGCTGC AACCCTCGCA GGCACCATGA AATCCCTCAT TGCCAAGGCG 
GTTGATCATG CTACTAATCG TACCCAGTTT GGGGACAAAA TTCACAACTT TGGGGTGATC 
CAGGAAAAGC TGGCTCGGAT GGCTATTCTG CAGTATGTGA CTGAGTCCAT GGCTTACATG 
CTGAGTGCCA ACATGGACCA GGGATTCAAA GACTTCCAGA TAGAAGCCGC CATCAGCAAA 
ATCTTTTGCT CGGAGGCGGC CTGGAAAGTG GCAGATGAGT GCATCCAAAT AATGGGGGGC 
ATGGGCTTCA TGAAGGAACC AGGGGTGGAG CGTGTGCTCC GAGATATTCG AATCTTCCGG 
ATCTTTGAAG GGGCAAATGA CATTCTTCGA CTGTTTGTGG CTCTGCAAGG CTGTATGGAC 
AAAGGAAAGG AACTCACTGG GCTGGGCAAT GCCCTGAAGA ATCCTTTTGG AAACGTTGGC 
CTCCTGATGG GAGAAGCAGG CAAACAGCTG AGACGGAGGA CAGGAATCGG CAGTGGTTTG 
AGTCTGTCAG GGATTGTCCA CCCAGAGTTA AGTCGCAGTG GTGAACTGGC AGTGCAGGCT 
CTGGATCAAT TTGCCACCGT GGTGGAGGCC AAGCTGGTGA AACACAAGAA AGGGATTGTC 
AACGAGCAGT TCCTGCTGCA GCGACTGGCG GACGGCGCCA TTGACCTCTA TGCCATGGTG 
GTGGTTCTGT CCAGAGCCTC AAGGTCCCTG AGTGAGGGCT ACCCGACGGC ACAGCATGAG 
AAAATGCTCT GTGATAGCTG GTGCATTGAG GCTGCAACTC GGATCCGAGA AAACATGGCC 
AGTCTGCAGT CCAGCCCTCA GCATCAGGAG CTCTTCCGGA ACTTCAGAAG CATCTCCAAG 
GCCATGGTGG AGAATGGTGG CCTGGTCACC GGTAACCCCC TGGGAATCTG A