LALBA cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	LALBA
Entrez Gene ID	281894
Full Name	lactalbumin alpha
Synonyms	a-LACTA,alfaLA
General protein information	Preferred Names lactalbumin alpha Names alpha-lactalbumin alpha lactabumin alpha lactalbumin alpha-lactalbumin A alpha-lactalbumin B alpha-lactalbumin protein variant D lactose synthase B protein
Gene Type	protein-coding
Organism	Bos taurus(cattle)
Genome	Chromosome: 5 Map Location: 5q21

mRNA and Protein(s)

mRNA	Protein	Name
NM_174378.2	NP_776803.1	alpha-lactalbumin precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	LALBA	NP_776803.1
Homo sapiens (human)	LALBA	NP_002280.1
Canis lupus familiaris (dog)	LALBA	NP_001003129.1
Rattus norvegicus (Norway rat)	Lalba	NP_036726.1
Pan troglodytes (chimpanzee)	LALBA	XP_001160401.1
	LALBA	XP_001102116.1
Mus musculus (house mouse)	Lalba	NP_034809.1

Gene Ontology
Bibliography

Related articles in PubMed

Dimeric but not monomeric ?-lactalbumin potentiates apoptosis by up regulation of ATF3 and reduction of histone deacetylase activity in primary and immortalised cells.
Sharp JA, Brennan AJ, Polekhina G, Ascher DB, Lefevre C, Nicholas KR
Cellular signalling3386-97(2017 May)

Thermal Stabilization of Proteins by Mono- and Oligosaccharides: Measurement and Analysis in the Context of an Excluded Volume Model.
Beg I, Minton AP, Hassan I, Islam A, Ahmad F
Biochemistry54(23)3594-603(2015 Jun)

Macromolecular crowding induces holo ?-lactalbumin aggregation by converting to its apo form.
Mittal S, Singh LR
PloS one9(12)e114029(2014)

Association between a polymorphism of the ?-lactalbumin gene and milk production traits in Chinese Holstein cows.
Zhou JP, Dong CH
Genetics and molecular research : GMR12(3)3375-82(2013 Sep)

Amino acid sequence determinants in self-assembly of insulin chiral amyloid superstructures: role of C-terminus of B-chain in association of fibrils.
Babenko V, Dzwolak W
FEBS letters587(6)625-30(2013 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study shows, for the first time, that alpha-lactalbumin isolated in a rare 28kDa dimeric form induces cell death, while 14kDa monomeric alpha-lactalbumin is inactive.
Title: Dimeric but not monomeric ?-lactalbumin potentiates apoptosis by up regulation of ATF3 and reduction of histone deacetylase activity in primary and immortalised cells.

Data suggest that stabilization of apo-alpha-lactalbumin by mono- and oligo-saccharides can be accounted for by simplified statistical-thermodynamic model considering volume exclusion deriving from steric repulsion between protein and saccharides.
Title: Thermal Stabilization of Proteins by Mono- and Oligosaccharides: Measurement and Analysis in the Context of an Excluded Volume Model.

Macromolecular crowding reduces the calcium binding affinity of holo-lactalbumin alpha resulting in the formation of apo-lactalbumin alpha (the calcium-depleted form of holo-LA) leading to aggregate formation.
Title: Macromolecular crowding induces holo ?-lactalbumin aggregation by converting to its apo form.

These findings suggest that the SNP alpha-LA2516 in the alpha-LA gene likely does not have potential as a molecular marker for milk production traits in Chinese Holstein cows.
Title: Association between a polymorphism of the ?-lactalbumin gene and milk production traits in Chinese Holstein cows.

alphaLA forms a misfolded disulfide bond shuffled isomer, X-alphaLA which represents a different low energy fold for the protein; binding calcium plays an important role in both maintaining the native structure of alphaLA and providing a mechanism for distinguishing between folded and misfolded species.
Title: Characterization of an alternative low energy fold for bovine ?-lactalbumin formed by disulfide bond shuffling.

The following LALBA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LALBA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa01388	NM_174378.2 Latest version!	Bos taurus lactalbumin alpha (LALBA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LALBA ( NM_174378.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776803.1 Bos taurus lactalbumin alpha (LALBA), mRNA.

Price & Availability↑

CloneID	OBa01388
Clone ID Related Accession (Same CDS sequence)	NM_174378.2
Accession Version	NM_174378.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 429bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Bos taurus(cattle)
Product	alpha-lactalbumin precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from J05147.1. On Jun 3, 2003 this sequence version replaced NM_174378.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: J05147.1, BC102173.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145447, SAMN03145452 [ECO:0000348] ##Evidence-Data-END##

ATGATGTCCT TTGTCTCTCT GCTCCTGGTA GGCATCCTAT TCCATGCCAC CCAGGCTGAA 
CAGTTAACAA AATGTGAGGT GTTCCGGGAG CTGAAAGACT TGAAGGGCTA CGGAGGTGTC 
AGTTTGCCTG AATGGGTCTG TACCACGTTT CATACCAGTG GTTATGACAC ACAAGCCATA 
GTACAAAACA ATGACAGCAC AGAATATGGA CTCTTCCAGA TAAATAATAA AATTTGGTGC 
AAAGACGACC AGAACCCTCA CTCAAGCAAC ATCTGTAACA TCTCCTGTGA CAAGTTCCTG 
GATGATGATC TTACTGATGA CATTATGTGT GTCAAGAAGA TTCTGGATAA AGTAGGAATT 
AACTACTGGT TGGCCCATAA AGCACTCTGT TCTGAGAAGC TGGATCAGTG GCTCTGTGAG 
AAGTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776803.1
CDS	28..456
Translation	MMSFVSLLLVGILFHATQAEQLTKCEVFRELKDLKGYGGVSLPEWVCTTFHTSGYDTQAIVQNNDSTEYGLFQINNKIWCKDDQNPHSSNICNISCDKFLDDDLTDDIMCVKKILDKVGINYWLAHKALCSEKLDQWLCEKL

Target ORF information:

RefSeq Version	NM_174378.2
Organism	Bos taurus(cattle)
Definition	Bos taurus lactalbumin alpha (LALBA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174378.2

ORF Insert Sequence:

ATGATGTCCT TTGTCTCTCT GCTCCTGGTA GGCATCCTAT TCCATGCCAC CCAGGCTGAA 
CAGTTAACAA AATGTGAGGT GTTCCGGGAG CTGAAAGACT TGAAGGGCTA CGGAGGTGTC 
AGTTTGCCTG AATGGGTCTG TACCACGTTT CATACCAGTG GTTATGACAC ACAAGCCATA 
GTACAAAACA ATGACAGCAC AGAATATGGA CTCTTCCAGA TAAATAATAA AATTTGGTGC 
AAAGACGACC AGAACCCTCA CTCAAGCAAC ATCTGTAACA TCTCCTGTGA CAAGTTCCTG 
GATGATGATC TTACTGATGA CATTATGTGT GTCAAGAAGA TTCTGGATAA AGTAGGAATT 
AACTACTGGT TGGCCCATAA AGCACTCTGT TCTGAGAAGC TGGATCAGTG GCTCTGTGAG 
AAGTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Dimeric but not monomeric ?-lactalbumin potentiates apoptosis by up regulation of ATF3 and reduction of histone deacetylase activity in primary and immortalised cells.
Cellular signalling3386-97(2017 May)
Sharp JA,Brennan AJ,Polekhina G,Ascher DB,Lefevre C,Nicholas KR

Thermal Stabilization of Proteins by Mono- and Oligosaccharides: Measurement and Analysis in the Context of an Excluded Volume Model.
Biochemistry54(23)3594-603(2015 Jun)
Beg I,Minton AP,Hassan I,Islam A,Ahmad F

Macromolecular crowding induces holo ?-lactalbumin aggregation by converting to its apo form.
PloS one9(12)e114029(2014)
Mittal S,Singh LR

Association between a polymorphism of the ?-lactalbumin gene and milk production traits in Chinese Holstein cows.
Genetics and molecular research : GMR12(3)3375-82(2013 Sep)
Zhou JP,Dong CH

Amino acid sequence determinants in self-assembly of insulin chiral amyloid superstructures: role of C-terminus of B-chain in association of fibrils.
FEBS letters587(6)625-30(2013 Mar)
Babenko V,Dzwolak W

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LALBA cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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