GNAO1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	GNAO1
Entrez Gene ID	281792
Full Name	G protein subunit alpha o1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_174325.1	NP_776750.1	guanine nucleotide-binding protein G(o) subunit alpha

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	gnao1	NP_001016995.1
	GNAO1	XP_001096035.2
Pan troglodytes (chimpanzee)	GNAO1	XP_510976.3
Canis lupus familiaris (dog)	GNAO1	XP_005617599.1
Bos taurus (cattle)	GNAO1	NP_776750.1
Gallus gallus (chicken)	GNAO1	NP_001264479.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005773	XP_315788.3
Caenorhabditis elegans (roundworm)	goa-1	NP_492108.1
Homo sapiens (human)	GNAO1	NP_066268.1
Mus musculus (house mouse)	Gnao1	NP_034438.1
Rattus norvegicus (Norway rat)	Gnao1	NP_059023.1
Danio rerio (zebrafish)	gnao1a	NP_957081.1
Drosophila melanogaster (fruit fly)	G-oalpha47A	NP_724934.1

Gene Ontology
Bibliography

Related articles in PubMed

Palmitoylation modification of Galpha(o) depresses its susceptibility to GAP-43 activation.
Yang H, Wan L, Song F, Wang M, Huang Y
The international journal of biochemistry & cell biology41(7)1495-501(2009 Jul)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Alternative splicing of the guanine nucleotide-binding regulatory protein Go alpha generates four distinct mRNAs.
Murtagh JJ Jr, Moss J, Vaughan M
Nucleic acids research22(5)842-9(1994 Mar)

Primary structure of bovine cerebellum GTP-binding protein G39 and its effect on the adenylate cyclase system.
Ovchinnikov YuA, Slepak VZ, Pronin AN, Shlensky AB, Levina NB, Voeikov VL, Lipkin VM
FEBS letters226(1)91-5(1987 Dec)

Hydroxylamine-stable covalent linkage of myristic acid in G0 alpha, a guanine nucleotide-binding protein of bovine brain.
Schultz AM, Tsai SC, Kung HF, Oroszlan S, Moss J, Vaughan M
Biochemical and biophysical research communications146(3)1234-9(1987 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results indicated the interaction of GAP-43 and Galpha(o) could accelerate conversion of depalmitoylated Galpha(o) but not palmitoylated Galpha(o) from oligomers to monomers, so as to increase the GTPgammaS binding activity of Galpha(o).
Title: Palmitoylation modification of Galpha(o) depresses its susceptibility to GAP-43 activation.

The following GNAO1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GNAO1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa41242	NM_174325.1 Latest version!	Bos taurus G protein subunit alpha o1 (GNAO1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GNAO1 ( NM_174325.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776750.1 Bos taurus G protein subunit alpha o1 (GNAO1), mRNA.

Price & Availability↑

CloneID	OBa41242
Clone ID Related Accession (Same CDS sequence)	NM_174325.1
Accession Version	NM_174325.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Bos taurus(cattle)
Product	guanine nucleotide-binding protein G(o) subunit alpha
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M16116.1. ##Evidence-Data-START## Transcript exon combination :: M16116.1, BC142217.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145449, SAMN03145464 [ECO:0000348] ##Evidence-Data-END##

ATGGGATGTA CTCTGAGCGC AGAGGAGCGA GCCGCCCTCG AGCGGAGCAA GGCGATTGAG 
AAAAACCTCA AAGAGGATGG CATCAGCGCC GCCAAAGACG TGAAATTACT CCTGCTGGGG 
GCTGGAGAAT CAGGAAAAAG CACCATTGTG AAGCAGATGA AGATCATCCA CGAAGACGGC 
TTCTCTGGCG AAGACGTGAA GCAGTACAAG CCCGTGGTCT ACAGCAACAC CATCCAGTCC 
CTGGCAGCCA TCGTCCGGGC CATGGACACT CTGGGCATCG AGTACGGTGA CAAGGAGCGA 
AAGGCCGACG CCAAGATGGT GTGTGACGTG GTGAGTCGGA TGGAAGACAC GGAGCCCTTC 
TCTCCAGAGC TGCTCTCCGC CATGATGCGA CTCTGGGGCG ACTCGGGGAT CCAGGAGTGC 
TTCAACAGGT CCCGGGAATA TCAGCTCAAC GACTCTGCCA AATACTACCT GGACAGCCTG 
GACCGGATCG GGGCCGCCGA CTACCAGCCC ACCGAGCAGG ACATCCTCCG AACCAGGGTC 
AAAACCACCG GCATCGTAGA AACCCACTTC ACATTCAAGA ACCTCCACTT CAGGCTGTTC 
GACGTCGGGG GCCAGCGGTC TGAGCGCAAG AAGTGGATTC ACTGCTTCGA GGACGTCACG 
GCCATCATCT TCTGTGTCGC GCTCAGCGGC TATGACCAGG TGCTCCACGA AGACGAGACC 
ACGAACCGCA TGCACGAGTC TCTCATGCTC TTCGACTCCA TCTGTAACAA CAAGTTCTTC 
ATCGATATCT CCATCATTCT CTTCCTCAAC AAGAAAGATC TCTTTGGGGA GAAGATCAAG 
AAGTCACCTC TGACCATCTG CTTTCCCGAG TACACAGGCT CCAACACCTA TGAAGACGCC 
GCCGCCTACA TCCAAGCACA ATTTGAAAGC AAAAACCGCT CACCCAACAA AGAAATTTAT 
TGTCACATGA CTTGTGCCAC AGACACGAAT AATATCCAGG TGGTATTCGA CGCTGTCACC 
GACATCATCA TTGCCAACAA CCTCCGGGGC TGCGGCTTGT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776750.1
CDS	202..1266
Translation	MGCTLSAEERAALERSKAIEKNLKEDGISAAKDVKLLLLGAGESGKSTIVKQMKIIHEDGFSGEDVKQYKPVVYSNTIQSLAAIVRAMDTLGIEYGDKERKADAKMVCDVVSRMEDTEPFSPELLSAMMRLWGDSGIQECFNRSREYQLNDSAKYYLDSLDRIGAADYQPTEQDILRTRVKTTGIVETHFTFKNLHFRLFDVGGQRSERKKWIHCFEDVTAIIFCVALSGYDQVLHEDETTNRMHESLMLFDSICNNKFFIDISIILFLNKKDLFGEKIKKSPLTICFPEYTGSNTYEDAAAYIQAQFESKNRSPNKEIYCHMTCATDTNNIQVVFDAVTDIIIANNLRGCGLY

Target ORF information:

RefSeq Version	NM_174325.1
Organism	Bos taurus(cattle)
Definition	Bos taurus G protein subunit alpha o1 (GNAO1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174325.1

ORF Insert Sequence:

ATGGGATGTA CTCTGAGCGC AGAGGAGCGA GCCGCCCTCG AGCGGAGCAA GGCGATTGAG 
AAAAACCTCA AAGAGGATGG CATCAGCGCC GCCAAAGACG TGAAATTACT CCTGCTGGGG 
GCTGGAGAAT CAGGAAAAAG CACCATTGTG AAGCAGATGA AGATCATCCA CGAAGACGGC 
TTCTCTGGCG AAGACGTGAA GCAGTACAAG CCCGTGGTCT ACAGCAACAC CATCCAGTCC 
CTGGCAGCCA TCGTCCGGGC CATGGACACT CTGGGCATCG AGTACGGTGA CAAGGAGCGA 
AAGGCCGACG CCAAGATGGT GTGTGACGTG GTGAGTCGGA TGGAAGACAC GGAGCCCTTC 
TCTCCAGAGC TGCTCTCCGC CATGATGCGA CTCTGGGGCG ACTCGGGGAT CCAGGAGTGC 
TTCAACAGGT CCCGGGAATA TCAGCTCAAC GACTCTGCCA AATACTACCT GGACAGCCTG 
GACCGGATCG GGGCCGCCGA CTACCAGCCC ACCGAGCAGG ACATCCTCCG AACCAGGGTC 
AAAACCACCG GCATCGTAGA AACCCACTTC ACATTCAAGA ACCTCCACTT CAGGCTGTTC 
GACGTCGGGG GCCAGCGGTC TGAGCGCAAG AAGTGGATTC ACTGCTTCGA GGACGTCACG 
GCCATCATCT TCTGTGTCGC GCTCAGCGGC TATGACCAGG TGCTCCACGA AGACGAGACC 
ACGAACCGCA TGCACGAGTC TCTCATGCTC TTCGACTCCA TCTGTAACAA CAAGTTCTTC 
ATCGATATCT CCATCATTCT CTTCCTCAAC AAGAAAGATC TCTTTGGGGA GAAGATCAAG 
AAGTCACCTC TGACCATCTG CTTTCCCGAG TACACAGGCT CCAACACCTA TGAAGACGCC 
GCCGCCTACA TCCAAGCACA ATTTGAAAGC AAAAACCGCT CACCCAACAA AGAAATTTAT 
TGTCACATGA CTTGTGCCAC AGACACGAAT AATATCCAGG TGGTATTCGA CGCTGTCACC 
GACATCATCA TTGCCAACAA CCTCCGGGGC TGCGGCTTGT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Palmitoylation modification of Galpha(o) depresses its susceptibility to GAP-43 activation.
The international journal of biochemistry & cell biology41(7)1495-501(2009 Jul)
Yang H,Wan L,Song F,Wang M,Huang Y

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Alternative splicing of the guanine nucleotide-binding regulatory protein Go alpha generates four distinct mRNAs.
Nucleic acids research22(5)842-9(1994 Mar)
Murtagh JJ Jr,Moss J,Vaughan M

Primary structure of bovine cerebellum GTP-binding protein G39 and its effect on the adenylate cyclase system.
FEBS letters226(1)91-5(1987 Dec)
Ovchinnikov YuA,Slepak VZ,Pronin AN,Shlensky AB,Levina NB,Voeikov VL,Lipkin VM

Hydroxylamine-stable covalent linkage of myristic acid in G0 alpha, a guanine nucleotide-binding protein of bovine brain.
Biochemical and biophysical research communications146(3)1234-9(1987 Aug)
Schultz AM,Tsai SC,Kung HF,Oroszlan S,Moss J,Vaughan M

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GNAO1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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