Gnao1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gnao1
Entrez Gene ID	50664
Full Name	G protein subunit alpha o1
Synonyms	Gnao,RATBPGTPC
General protein information	Preferred Names G protein subunit alpha o1 Names guanine nucleotide-binding protein G(o) subunit alpha GTP-binding protein alpha o guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O guanine nucleotide binding protein, alpha O polypeptide 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 19 Map Location: 19p12
Summary	guanine nucleotide binding protein involved in mediating signal transduction between membrane receptors and intracellular second messengers [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_008772321.2	XP_008770543.1	guanine nucleotide-binding protein G(o) subunit alpha isoform X1
NM_017327.1	NP_059023.1	guanine nucleotide-binding protein G(o) subunit alpha

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	gnao1	NP_001016995.1
	GNAO1	XP_001096035.2
Pan troglodytes (chimpanzee)	GNAO1	XP_510976.3
Canis lupus familiaris (dog)	GNAO1	XP_005617599.1
Bos taurus (cattle)	GNAO1	NP_776750.1
Gallus gallus (chicken)	GNAO1	NP_001264479.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005773	XP_315788.3
Caenorhabditis elegans (roundworm)	goa-1	NP_492108.1
Homo sapiens (human)	GNAO1	NP_066268.1
Mus musculus (house mouse)	Gnao1	NP_034438.1
Rattus norvegicus (Norway rat)	Gnao1	NP_059023.1
Danio rerio (zebrafish)	gnao1a	NP_957081.1
Drosophila melanogaster (fruit fly)	G-oalpha47A	NP_724934.1

Gene Ontology
Bibliography

Related articles in PubMed

RNA-Sequencing Analyses Demonstrate the Involvement of Canonical Transient Receptor Potential Channels in Rat Tooth Germ Development.
Yang J, Cai W, Lu X, Liu S, Zhao S
Frontiers in physiology8455(2017)

The Orphan G Protein-coupled Receptor GPR17 Negatively Regulates Oligodendrocyte Differentiation via G?i/o and Its Downstream Effector Molecules.
Simon K, Hennen S, Merten N, Bl?ttermann S, Gillard M, Kostenis E, Gomeza J
The Journal of biological chemistry291(2)705-18(2016 Jan)

Functionally selective dopamine D?, D? receptor partial agonists.
M?ller D, Kling RC, Skultety M, Leuner K, H?bner H, Gmeiner P
Journal of medicinal chemistry57(11)4861-75(2014 Jun)

Integrating energy calculations with functional assays to decipher the specificity of G protein-RGS protein interactions.
Kosloff M, Travis AM, Bosch DE, Siderovski DP, Arshavsky VY
Nature structural & molecular biology18(7)846-53(2011 Jun)

Sex differences in corticotropin-releasing factor receptor signaling and trafficking: potential role in female vulnerability to stress-related psychopathology.
Bangasser DA, Curtis A, Reyes BA, Bethea TT, Parastatidis I, Ischiropoulos H, Van Bockstaele EJ, Valentino RJ
Molecular psychiatry15(9)877, 896-904(2010 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

stimulation of GPR17 by the small molecule agonist MDL29,951 (2-carboxy-4,6-dichloro-1H-indole-3-propionic acid) decreases myelin basic protein expression levels mainly by triggering the Galphai/o signaling pathway.
Title: The Orphan G Protein-coupled Receptor GPR17 Negatively Regulates Oligodendrocyte Differentiation via G?i/o and Its Downstream Effector Molecules.

Studies demonstrate central Galphai/Galphao activity mediates intracerebroventricular nociceptin's cardiovascular depressor function.
Title: Central G-alpha subunit protein-mediated control of cardiovascular function, urine output, and vasopressin secretion in conscious Sprague-Dawley rats.

Results suggest that opioid receptor-like (ORL1) receptor utilizes both G(oA) and G(oB) for signal transduction.
Title: Opioid receptor-like (ORL1) receptor utilizes both G(oA) and G(oB) for signal transduction.

data presented in support of the interpretation that impaired inhibitory G protein function in diabetic neuropathy involves a pathway that includes activation of protein kinase C and phosphorylation of the Goalpha subunit
Title: Diabetic neuropathy: inhibitory G protein dysfunction involves PKC-dependent phosphorylation of Goalpha.

Prepulse inhibition of acoustic startle response mediated by D(2)-like receptor activation in nucelus accumbens depends on coupling to G(i) and G(o) proteins, alteration of which could cause sensorimotor gating deficits in schizophrenia.
Title: Reduced G(i) and G(o) protein function in the rat nucleus accumbens attenuates sensorimotor gating deficits.

The following Gnao1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gnao1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa03657	NM_017327.1 Latest version!	Rattus norvegicus G protein subunit alpha o1 (Gnao1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa43393	XM_008772321.2 Latest version!	Rattus norvegicus G protein subunit alpha o1 (Gnao1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gnao1 ( NM_017327.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_059023.1 Rattus norvegicus G protein subunit alpha o1 (Gnao1), mRNA.

Price & Availability↑

CloneID	ORa03657
Clone ID Related Accession (Same CDS sequence)	NM_017327.1
Accession Version	NM_017327.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	guanine nucleotide-binding protein G(o) subunit alpha
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M17526.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M17526.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296 [ECO:0000348] ##Evidence-Data-END##

ATGGGATGTA CTCTGAGCGC AGAGGAGAGA GCCGCCCTCG AGCGGAGCAA GGCGATTGAG 
AAAAATCTCA AAGAAGATGG CATCAGCGCC GCCAAAGACG TGAAATTACT CCTGCTGGGG 
GCTGGAGAAT CAGGAAAAAG CACCATTGTG AAGCAGATGA AGATCATCCA TGAAGATGGC 
TTCTCTGGAG AAGACGTAAA GCAGTACAAG CCTGTCGTCT ACAGCAACAC CATCCAGTCT 
CTGGCAGCCA TTGTGCGGGC CATGGATACT CTGGGCGTGG AGTATGGTGA CAAGGAGAGG 
AAGGCAGACT CCAAGATGGT GTGTGACGTG GTGAGTCGCA TGGAGGACAC TGAACCATTC 
TCTGCAGAAC TGCTTTCTGC CATGATGCGA CTCTGGGGCG ACTCGGGGAT CCAGGAGTGC 
TTCAACCGAT CTCGGGAGTA TCAGCTCAAC GACTCTGCCA AATACTACCT GGACAGCTTG 
GATCGGATTG GAGCCGCTGA CTACCAGCCC ACCGAGCAGG ACATCCTCCG AACCAGGGTC 
AAAACAACTG GCATCGTAGA AACCCACTTC ACCTTCAAGA ACCTCCACTT CAGGCTGTTT 
GACGTTGGGG GCCAGCGATC TGAACGTAAG AAGTGGATCC ACTGCTTCGA GGATGTCACG 
GCCATCATCT TCTGTGTCGC ACTCAGCGGC TATGACCAGG TGCTCCACGA GGACGAAACC 
ACGAACCGCA TGCACGAGTC TCTCATGCTC TTCGACTCCA TCTGTAACAA CAAGTTTTTC 
ATCGATACCT CCATCATTCT CTTCCTCAAC AAGAAAGACC TCTTTGGCGA GAAGATTAAG 
AAGTCACCCT TGACCATCTG CTTTCCTGAA TACCCAGGCT CCAACACCTA TGAAGACGCA 
GCTGCCTACA TCCAAACACA GTTTGAAAGC AAAAACCGCT CACCCAACAA AGAAATTTAC 
TGTCACATGA CTTGTGCCAC AGACACGAAT AATATCCAGG TGGTATTCGA CGCCGTCACC 
GACATCATCA TTGCCAACAA TCTCCGGGGC TGTGGCTTGT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_059023.1
CDS	707..1771
Translation	MGCTLSAEERAALERSKAIEKNLKEDGISAAKDVKLLLLGAGESGKSTIVKQMKIIHEDGFSGEDVKQYKPVVYSNTIQSLAAIVRAMDTLGVEYGDKERKADSKMVCDVVSRMEDTEPFSAELLSAMMRLWGDSGIQECFNRSREYQLNDSAKYYLDSLDRIGAADYQPTEQDILRTRVKTTGIVETHFTFKNLHFRLFDVGGQRSERKKWIHCFEDVTAIIFCVALSGYDQVLHEDETTNRMHESLMLFDSICNNKFFIDTSIILFLNKKDLFGEKIKKSPLTICFPEYPGSNTYEDAAAYIQTQFESKNRSPNKEIYCHMTCATDTNNIQVVFDAVTDIIIANNLRGCGLY

Target ORF information:

RefSeq Version	NM_017327.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus G protein subunit alpha o1 (Gnao1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017327.1

ORF Insert Sequence:

ATGGGATGTA CTCTGAGCGC AGAGGAGAGA GCCGCCCTCG AGCGGAGCAA GGCGATTGAG 
AAAAATCTCA AAGAAGATGG CATCAGCGCC GCCAAAGACG TGAAATTACT CCTGCTGGGG 
GCTGGAGAAT CAGGAAAAAG CACCATTGTG AAGCAGATGA AGATCATCCA TGAAGATGGC 
TTCTCTGGAG AAGACGTAAA GCAGTACAAG CCTGTCGTCT ACAGCAACAC CATCCAGTCT 
CTGGCAGCCA TTGTGCGGGC CATGGATACT CTGGGCGTGG AGTATGGTGA CAAGGAGAGG 
AAGGCAGACT CCAAGATGGT GTGTGACGTG GTGAGTCGCA TGGAGGACAC TGAACCATTC 
TCTGCAGAAC TGCTTTCTGC CATGATGCGA CTCTGGGGCG ACTCGGGGAT CCAGGAGTGC 
TTCAACCGAT CTCGGGAGTA TCAGCTCAAC GACTCTGCCA AATACTACCT GGACAGCTTG 
GATCGGATTG GAGCCGCTGA CTACCAGCCC ACCGAGCAGG ACATCCTCCG AACCAGGGTC 
AAAACAACTG GCATCGTAGA AACCCACTTC ACCTTCAAGA ACCTCCACTT CAGGCTGTTT 
GACGTTGGGG GCCAGCGATC TGAACGTAAG AAGTGGATCC ACTGCTTCGA GGATGTCACG 
GCCATCATCT TCTGTGTCGC ACTCAGCGGC TATGACCAGG TGCTCCACGA GGACGAAACC 
ACGAACCGCA TGCACGAGTC TCTCATGCTC TTCGACTCCA TCTGTAACAA CAAGTTTTTC 
ATCGATACCT CCATCATTCT CTTCCTCAAC AAGAAAGACC TCTTTGGCGA GAAGATTAAG 
AAGTCACCCT TGACCATCTG CTTTCCTGAA TACCCAGGCT CCAACACCTA TGAAGACGCA 
GCTGCCTACA TCCAAACACA GTTTGAAAGC AAAAACCGCT CACCCAACAA AGAAATTTAC 
TGTCACATGA CTTGTGCCAC AGACACGAAT AATATCCAGG TGGTATTCGA CGCCGTCACC 
GACATCATCA TTGCCAACAA TCTCCGGGGC TGTGGCTTGT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gnao1 ( XM_008772321.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008770543.1 Rattus norvegicus G protein subunit alpha o1 (Gnao1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa43393
Clone ID Related Accession (Same CDS sequence)	XM_008772321.2
Accession Version	XM_008772321.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	guanine nucleotide-binding protein G(o) subunit alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008772321.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGATGTA CTCTGAGCGC AGAGGAGAGA GCCGCCCTCG AGCGGAGCAA GGCGATTGAG 
AAAAATCTCA AAGAAGATGG CATCAGCGCC GCCAAAGACG TGAAATTACT CCTGCTGGGG 
GCTGGAGAAT CAGGAAAAAG CACCATTGTG AAGCAGATGA AGATCATCCA TGAAGATGGC 
TTCTCTGGAG AAGACGTAAA GCAGTACAAG CCTGTCGTCT ACAGCAACAC CATCCAGTCT 
CTGGCAGCCA TTGTGCGGGC CATGGATACT CTGGGCGTGG AGTATGGTGA CAAGGAGAGG 
AAGGCAGACT CCAAGATGGT GTGTGACGTG GTGAGTCGCA TGGAGGACAC TGAACCATTC 
TCTGCAGAAC TGCTTTCTGC CATGATGCGA CTCTGGGGCG ACTCGGGGAT CCAGGAGTGC 
TTCAACCGAT CTCGGGAGTA TCAGCTCAAC GACTCTGCCA AATACTACCT GGACAGCTTG 
GATCGGATTG GAGCCGCTGA CTACCAGCCC ACCGAGCAGG ACATCCTCCG AACCAGGGTC 
AAAACAACTG GCATCGTAGA AACCCACTTC ACCTTCAAGA ACCTCCACTT CAGGCTGTTT 
GACGTTGGGG GCCAGCGATC TGAACGTAAG AAGTGGATCC ACTGCTTCGA GGATGTCACG 
GCCATCATCT TCTGTGTCGC ACTCAGCGGC TATGACCAGG TGCTCCACGA GGACGAAACC 
ACGAACCGCA TGCATGAGTC TCTGAAGCTT TTTGACAGCA TCTGCAACAA CAAGTGGTTC 
ACAGACACTT CGATCATCCT GTTTCTCAAC AAGAAGGACA TATTTGAGGA GAAGATCAAG 
AAGTCCCCAC TCACCATCTG CTTCCCTGAA TACACAGGCC CCAGTGCCTT CACAGAAGCT 
GTGGCTCATA TCCAAGGGCA GTATGAGAGT AAGAATAAGT CAGCTCACAA GGAGGTCTAC 
AGCCATGTCA CCTGTGCCAC GGACACCAAC AACATCCAGT TCGTCTTTGA TGCCGTGACA 
GATGTCATCA TCGCCAAAAA CCTGCGGGGC TGTGGCCTCT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008770543.1
CDS	22..1086
Translation	MGCTLSAEERAALERSKAIEKNLKEDGISAAKDVKLLLLGAGESGKSTIVKQMKIIHEDGFSGEDVKQYKPVVYSNTIQSLAAIVRAMDTLGVEYGDKERKADSKMVCDVVSRMEDTEPFSAELLSAMMRLWGDSGIQECFNRSREYQLNDSAKYYLDSLDRIGAADYQPTEQDILRTRVKTTGIVETHFTFKNLHFRLFDVGGQRSERKKWIHCFEDVTAIIFCVALSGYDQVLHEDETTNRMHESLKLFDSICNNKWFTDTSIILFLNKKDIFEEKIKKSPLTICFPEYTGPSAFTEAVAHIQGQYESKNKSAHKEVYSHVTCATDTNNIQFVFDAVTDVIIAKNLRGCGLY

Target ORF information:

RefSeq Version	XM_008772321.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus G protein subunit alpha o1 (Gnao1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008772321.2

ORF Insert Sequence:

ATGGGATGTA CTCTGAGCGC AGAGGAGAGA GCCGCCCTCG AGCGGAGCAA GGCGATTGAG 
AAAAATCTCA AAGAAGATGG CATCAGCGCC GCCAAAGACG TGAAATTACT CCTGCTGGGG 
GCTGGAGAAT CAGGAAAAAG CACCATTGTG AAGCAGATGA AGATCATCCA TGAAGATGGC 
TTCTCTGGAG AAGACGTAAA GCAGTACAAG CCTGTCGTCT ACAGCAACAC CATCCAGTCT 
CTGGCAGCCA TTGTGCGGGC CATGGATACT CTGGGCGTGG AGTATGGTGA CAAGGAGAGG 
AAGGCAGACT CCAAGATGGT GTGTGACGTG GTGAGTCGCA TGGAGGACAC TGAACCATTC 
TCTGCAGAAC TGCTTTCTGC CATGATGCGA CTCTGGGGCG ACTCGGGGAT CCAGGAGTGC 
TTCAACCGAT CTCGGGAGTA TCAGCTCAAC GACTCTGCCA AATACTACCT GGACAGCTTG 
GATCGGATTG GAGCCGCTGA CTACCAGCCC ACCGAGCAGG ACATCCTCCG AACCAGGGTC 
AAAACAACTG GCATCGTAGA AACCCACTTC ACCTTCAAGA ACCTCCACTT CAGGCTGTTT 
GACGTTGGGG GCCAGCGATC TGAACGTAAG AAGTGGATCC ACTGCTTCGA GGATGTCACG 
GCCATCATCT TCTGTGTCGC ACTCAGCGGC TATGACCAGG TGCTCCACGA GGACGAAACC 
ACGAACCGCA TGCATGAGTC TCTGAAGCTT TTTGACAGCA TCTGCAACAA CAAGTGGTTC 
ACAGACACTT CGATCATCCT GTTTCTCAAC AAGAAGGACA TATTTGAGGA GAAGATCAAG 
AAGTCCCCAC TCACCATCTG CTTCCCTGAA TACACAGGCC CCAGTGCCTT CACAGAAGCT 
GTGGCTCATA TCCAAGGGCA GTATGAGAGT AAGAATAAGT CAGCTCACAA GGAGGTCTAC 
AGCCATGTCA CCTGTGCCAC GGACACCAAC AACATCCAGT TCGTCTTTGA TGCCGTGACA 
GATGTCATCA TCGCCAAAAA CCTGCGGGGC TGTGGCCTCT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RNA-Sequencing Analyses Demonstrate the Involvement of Canonical Transient Receptor Potential Channels in Rat Tooth Germ Development.
Frontiers in physiology8455(2017)
Yang J,Cai W,Lu X,Liu S,Zhao S

The Orphan G Protein-coupled Receptor GPR17 Negatively Regulates Oligodendrocyte Differentiation via G?i/o and Its Downstream Effector Molecules.
The Journal of biological chemistry291(2)705-18(2016 Jan)
Simon K,Hennen S,Merten N,Bl?ttermann S,Gillard M,Kostenis E,Gomeza J

Functionally selective dopamine D?, D? receptor partial agonists.
Journal of medicinal chemistry57(11)4861-75(2014 Jun)
M?ller D,Kling RC,Skultety M,Leuner K,H?bner H,Gmeiner P

Integrating energy calculations with functional assays to decipher the specificity of G protein-RGS protein interactions.
Nature structural & molecular biology18(7)846-53(2011 Jun)
Kosloff M,Travis AM,Bosch DE,Siderovski DP,Arshavsky VY

Sex differences in corticotropin-releasing factor receptor signaling and trafficking: potential role in female vulnerability to stress-related psychopathology.
Molecular psychiatry15(9)877, 896-904(2010 Sep)
Bangasser DA,Curtis A,Reyes BA,Bethea TT,Parastatidis I,Ischiropoulos H,Van Bockstaele EJ,Valentino RJ

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Gnao1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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