VARS2 cDNA ORF clone, Bos taurus(cattle)

The following VARS2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VARS2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa32095 XM_024983370.1
Latest version!
Bos taurus valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa32095 XM_010818310.2
Latest version!
Bos taurus valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa60390 XM_005223583.4
Latest version!
Bos taurus valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa39654 NM_001256544.1
Latest version!
Bos taurus valyl-tRNA synthetase 2, mitochondrial (VARS2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa39654 XM_005223581.4
Latest version!
Bos taurus valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa60389 XM_005223582.4
Latest version!
Bos taurus valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
OBa39654 XM_005223581.3 Bos taurus valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa60389 XM_005223582.3 Bos taurus valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa60390 XM_005223583.3 Bos taurus valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa32095
    Clone ID Related Accession (Same CDS sequence) XM_024983370.1 , XM_010818310.2
    Accession Version XM_024983370.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2847bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product valine--tRNA ligase, mitochondrial isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAAATCCC CACGGAACAT GGAGAAAAGA AAGGCCAGGC TGCCCCAGGC CACAGGGGAG 
    ACCTTTTCCA TGTGTATCCC ACCCCCTAAT GTCACAGGCT CCCTGCACAT TGGGCATGCG
    CTGACCGTGG CCATACAGGA TGCTCTCGTG CGCTGGCACC GGATGCGTGG GGATCGGGTG
    CTGTGGGTCC CTGGCTCAGA TCATGCAGGG ATTGCAACCC AAGCTGTGGT GGAGAAACAG
    CTGTGGAAGG AGCGAGGCGT GAGGAGACAT GAGCTGAGCC GGGAAGACTT CCTTAGGGAG
    GTGTGGAAGT GGAAGGAGGA GAAAGGTGGC GAGATCTGTG AGCAGCTCCG AGCGCTGGGG
    GCCTCCCTGG ACTGGGACCG AGAGTGCTTT ACCATGGATA CCGGCTCCTC AGTGGCCGTG
    ACAGAAGCTT TCGTGCGGCT CTACAAGGCT GGGTTGTTGT ACCGGAGCCG GCAGCTGGTC
    AATTGGTCGT GTGCCTTACG CTCCGCCATC TCGGATATTG AGGTGGAGAG CCGGCCCCTC
    CCTGGCCGCA CGGAGCTTCA GTTGCCTGGC TGCCCCACCC CCGTGTCTTT CGGCCTCCTC
    TTTTCTGTGG CCTTCCCCGT GGATGGAGAG CCTGATGCAG AGGTTGTGGT GGGAACCACG
    AGGCCAGAGA CGCTGCCTGG AGACGTGGCT GTGGCGGTCC ATCCTGATGA TTCCCGCTAC
    ACGCATCTAC ATGGGCGACA ACTCTGTCAT CCCTTGACGG GGCAGCTTCT CCCTCTTATC
    ACAGACTGCA CTGTTCAGCC ACACCTGGGC ACAGGGGCGG TGAAGGTGAC TCCAGCGCAC
    AGCCCTGCCG ATGCTGAGCT GGGGGCCCGA CACGGCCTGA GCCCCCGGAG TGTCATTGCG
    GAGGATGGGA CCATGACCGC CCTTTGTGAG GACTGGCTGC AGGGTCTTCA CCGATTTGTG
    GCCCGGGAAA AGATTCTGTC AGCACTGAGG GAGCGGGGCC TGTTCCGGGG CCTCCAGAAC
    CACCCCATGG TGCTGCCCAT TTGCAGCCGT TCCGGGGACG TGGTAGAGTA CCTACTGAAG
    AGCCAGTGGT TTGTCCGCTG CCGGGAAATG GGGGAGCGAG CCGCCAAGGC CGTGGAGTCG
    GGGGCCCTGG ACCTCAGTCC CTCCTTCCAC CAGAAGAACT GGCAGCACTG GTTTTCCCAC
    ATTGGGGACT GGTGTGTCTC CCGGCAGCTG TGGTGGGGCC ATCGGATTCC AGCCTACCTG
    GTTGTAGAGG AACATGCAAA GGGTGAAACG GAGGACTTCT GGGTGGTCGG GCGGACAGAG
    GCTGAGGCCA GAGAAGTAGC TGCAGAATTA ACAGGGAGAC CAGGGGCGGA GCTGACCCTG
    GAGAGGGACC CTGATGTCCT GGACACGTGG TTCTCCTCCG CTCTTTTCCC CTTTTCTGCC
    CTGGGCTGGC CCCGAGAGAC CCCGGACCTG GCTCGTTTCT ACCCCCTGTC ACTTTTGGAA
    ACGGGCAGTG ACCTCCTGCT CTTCTGGGTG GGCCGCATGG TCATGTTGGG GACCCAGCTC
    ACAGGGCAGC TCCCCTTCAG CAAGGTCCTG CTTCACTCCA TGGTCCGGGA CAGGCAGGGC
    CGGAAGATGA GCAAGTCCCT GGGGAACGTG CTGGACCCAC GGGACATCAT CAGTGGGGTG
    GAGCTGCAGG TACTGCAGGA CAATCTGAGG GATGGAAACT TGGACCCCGC AGAGCTGGCG
    ATTGCAGCCT CAGCGCAGAG AAAGGACTTC CCTCATGGGA TCCCCGAGTG TGGGACAGAT
    GCTCTGAGGT TCACCCTGTG CTCCCATGGG GCCCTGGGAG GTGACTTGCA CCTGTCCGTC
    TCTGAGGTCC TGAGCTTCCG ACACTTCTGC AATAAGATCT GGAATGCCCT GCGCTTTATC
    CTGAATGCTC TTGGGGAGGA ATTCATACCC CAACCTGCGG AGGAGCTGTC CCCCGCCTCC
    CGCATGGACA CCTGGATCCT CAGCTGCCTG GCCCGCACGG CCCAGGACTG CGAGCGGGGC
    TTCCTCACCC GCGAGCTCTC GCTCGTCACC CACGCCCTGC ACCACTTCTG GCTGCACAAC
    CTCTGTGATG TCTACTTGGA GGCTGTGAAG CCGGTGCTAT CACACTCTCC CCGCCCTCTG
    GAGCCGCCTC AGGTCCTGTT CTTCTGCGCA GACGTCGGTC TCCGCCTCCT CGCCCCGTTG
    ATGCCTTTCC TGGCTGAAGA GCTCTGGCAG AGGCTGCCCC CCAGGCCTGG TGGTCCCTCT
    GCCCCCAGCA TCTGTGTTGC CCCCTACCCA AGTGCCCGCA GCTTGGAGCA CTGGCACCAG
    CCTGAGCTGG AGAGGCGCTT CTCCCGGGTT CAGGAGTCCG TGCAGGTGCT CAGGGCTCTG
    CGCGCCACCT ACCAGCTCAC CAAGGCCCGG CCCCGAGTGC TGCTGCAGAG CTCAGAGCCA
    GGCGAGCAGG GCCTCTTTGA GGCCTTCCTG GAGCCGCTGG GCATCCTGGG CTACTGTGGG
    GCGGTGGGCC TTCTACCCCC TGGTGCAGCA GCCCCCTCAG GCTGGGTCCA GGCCGCCCTC
    AGTGACACCA GTCAGGCGTA TATGGAGCTG CAGGGCCTGG TGGACCCCCA GACCCACCTA
    CCTCGGCTGG CTGCCCGAAG ACACAAGTTG CAGAAGCAGC TTGATGGCCT CCTAGCCCGG
    CCCCCATCCG AGGGACAGGC GGAGACTCAG AGGCAGCAGA GGCTTTCTTC CCTCCAGTTG
    GAGTTGTCAA AACTGGACAA GGCGGCCTCT CACCTCCAGC AGCTGATGGA TGATTTTCCA
    AGCCCTGGGA GCTCACCATC CCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024839138.1
    CDS74..2920
    Translation

    Target ORF information:

    RefSeq Version XM_024983370.1
    Organism Bos taurus(cattle)
    Definition Bos taurus valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024983370.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAAATCCC CACGGAACAT GGAGAAAAGA AAGGCCAGGC TGCCCCAGGC CACAGGGGAG 
    ACCTTTTCCA TGTGTATCCC ACCCCCTAAT GTCACAGGCT CCCTGCACAT TGGGCATGCG
    CTGACCGTGG CCATACAGGA TGCTCTCGTG CGCTGGCACC GGATGCGTGG GGATCGGGTG
    CTGTGGGTCC CTGGCTCAGA TCATGCAGGG ATTGCAACCC AAGCTGTGGT GGAGAAACAG
    CTGTGGAAGG AGCGAGGCGT GAGGAGACAT GAGCTGAGCC GGGAAGACTT CCTTAGGGAG
    GTGTGGAAGT GGAAGGAGGA GAAAGGTGGC GAGATCTGTG AGCAGCTCCG AGCGCTGGGG
    GCCTCCCTGG ACTGGGACCG AGAGTGCTTT ACCATGGATA CCGGCTCCTC AGTGGCCGTG
    ACAGAAGCTT TCGTGCGGCT CTACAAGGCT GGGTTGTTGT ACCGGAGCCG GCAGCTGGTC
    AATTGGTCGT GTGCCTTACG CTCCGCCATC TCGGATATTG AGGTGGAGAG CCGGCCCCTC
    CCTGGCCGCA CGGAGCTTCA GTTGCCTGGC TGCCCCACCC CCGTGTCTTT CGGCCTCCTC
    TTTTCTGTGG CCTTCCCCGT GGATGGAGAG CCTGATGCAG AGGTTGTGGT GGGAACCACG
    AGGCCAGAGA CGCTGCCTGG AGACGTGGCT GTGGCGGTCC ATCCTGATGA TTCCCGCTAC
    ACGCATCTAC ATGGGCGACA ACTCTGTCAT CCCTTGACGG GGCAGCTTCT CCCTCTTATC
    ACAGACTGCA CTGTTCAGCC ACACCTGGGC ACAGGGGCGG TGAAGGTGAC TCCAGCGCAC
    AGCCCTGCCG ATGCTGAGCT GGGGGCCCGA CACGGCCTGA GCCCCCGGAG TGTCATTGCG
    GAGGATGGGA CCATGACCGC CCTTTGTGAG GACTGGCTGC AGGGTCTTCA CCGATTTGTG
    GCCCGGGAAA AGATTCTGTC AGCACTGAGG GAGCGGGGCC TGTTCCGGGG CCTCCAGAAC
    CACCCCATGG TGCTGCCCAT TTGCAGCCGT TCCGGGGACG TGGTAGAGTA CCTACTGAAG
    AGCCAGTGGT TTGTCCGCTG CCGGGAAATG GGGGAGCGAG CCGCCAAGGC CGTGGAGTCG
    GGGGCCCTGG ACCTCAGTCC CTCCTTCCAC CAGAAGAACT GGCAGCACTG GTTTTCCCAC
    ATTGGGGACT GGTGTGTCTC CCGGCAGCTG TGGTGGGGCC ATCGGATTCC AGCCTACCTG
    GTTGTAGAGG AACATGCAAA GGGTGAAACG GAGGACTTCT GGGTGGTCGG GCGGACAGAG
    GCTGAGGCCA GAGAAGTAGC TGCAGAATTA ACAGGGAGAC CAGGGGCGGA GCTGACCCTG
    GAGAGGGACC CTGATGTCCT GGACACGTGG TTCTCCTCCG CTCTTTTCCC CTTTTCTGCC
    CTGGGCTGGC CCCGAGAGAC CCCGGACCTG GCTCGTTTCT ACCCCCTGTC ACTTTTGGAA
    ACGGGCAGTG ACCTCCTGCT CTTCTGGGTG GGCCGCATGG TCATGTTGGG GACCCAGCTC
    ACAGGGCAGC TCCCCTTCAG CAAGGTCCTG CTTCACTCCA TGGTCCGGGA CAGGCAGGGC
    CGGAAGATGA GCAAGTCCCT GGGGAACGTG CTGGACCCAC GGGACATCAT CAGTGGGGTG
    GAGCTGCAGG TACTGCAGGA CAATCTGAGG GATGGAAACT TGGACCCCGC AGAGCTGGCG
    ATTGCAGCCT CAGCGCAGAG AAAGGACTTC CCTCATGGGA TCCCCGAGTG TGGGACAGAT
    GCTCTGAGGT TCACCCTGTG CTCCCATGGG GCCCTGGGAG GTGACTTGCA CCTGTCCGTC
    TCTGAGGTCC TGAGCTTCCG ACACTTCTGC AATAAGATCT GGAATGCCCT GCGCTTTATC
    CTGAATGCTC TTGGGGAGGA ATTCATACCC CAACCTGCGG AGGAGCTGTC CCCCGCCTCC
    CGCATGGACA CCTGGATCCT CAGCTGCCTG GCCCGCACGG CCCAGGACTG CGAGCGGGGC
    TTCCTCACCC GCGAGCTCTC GCTCGTCACC CACGCCCTGC ACCACTTCTG GCTGCACAAC
    CTCTGTGATG TCTACTTGGA GGCTGTGAAG CCGGTGCTAT CACACTCTCC CCGCCCTCTG
    GAGCCGCCTC AGGTCCTGTT CTTCTGCGCA GACGTCGGTC TCCGCCTCCT CGCCCCGTTG
    ATGCCTTTCC TGGCTGAAGA GCTCTGGCAG AGGCTGCCCC CCAGGCCTGG TGGTCCCTCT
    GCCCCCAGCA TCTGTGTTGC CCCCTACCCA AGTGCCCGCA GCTTGGAGCA CTGGCACCAG
    CCTGAGCTGG AGAGGCGCTT CTCCCGGGTT CAGGAGTCCG TGCAGGTGCT CAGGGCTCTG
    CGCGCCACCT ACCAGCTCAC CAAGGCCCGG CCCCGAGTGC TGCTGCAGAG CTCAGAGCCA
    GGCGAGCAGG GCCTCTTTGA GGCCTTCCTG GAGCCGCTGG GCATCCTGGG CTACTGTGGG
    GCGGTGGGCC TTCTACCCCC TGGTGCAGCA GCCCCCTCAG GCTGGGTCCA GGCCGCCCTC
    AGTGACACCA GTCAGGCGTA TATGGAGCTG CAGGGCCTGG TGGACCCCCA GACCCACCTA
    CCTCGGCTGG CTGCCCGAAG ACACAAGTTG CAGAAGCAGC TTGATGGCCT CCTAGCCCGG
    CCCCCATCCG AGGGACAGGC GGAGACTCAG AGGCAGCAGA GGCTTTCTTC CCTCCAGTTG
    GAGTTGTCAA AACTGGACAA GGCGGCCTCT CACCTCCAGC AGCTGATGGA TGATTTTCCA
    AGCCCTGGGA GCTCACCATC CCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa32095
    Clone ID Related Accession (Same CDS sequence) XM_024983370.1 , XM_010818310.2
    Accession Version XM_010818310.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2847bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product valine--tRNA ligase, mitochondrial isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104557.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010818310.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAAATCCC CACGGAACAT GGAGAAAAGA AAGGCCAGGC TGCCCCAGGC CACAGGGGAG 
    ACCTTTTCCA TGTGTATCCC ACCCCCTAAT GTCACAGGCT CCCTGCACAT TGGGCATGCG
    CTGACCGTGG CCATACAGGA TGCTCTCGTG CGCTGGCACC GGATGCGTGG GGATCGGGTG
    CTGTGGGTCC CTGGCTCAGA TCATGCAGGG ATTGCAACCC AAGCTGTGGT GGAGAAACAG
    CTGTGGAAGG AGCGAGGCGT GAGGAGACAT GAGCTGAGCC GGGAAGACTT CCTTAGGGAG
    GTGTGGAAGT GGAAGGAGGA GAAAGGTGGC GAGATCTGTG AGCAGCTCCG AGCGCTGGGG
    GCCTCCCTGG ACTGGGACCG AGAGTGCTTT ACCATGGATA CCGGCTCCTC AGTGGCCGTG
    ACAGAAGCTT TCGTGCGGCT CTACAAGGCT GGGTTGTTGT ACCGGAGCCG GCAGCTGGTC
    AATTGGTCGT GTGCCTTACG CTCCGCCATC TCGGATATTG AGGTGGAGAG CCGGCCCCTC
    CCTGGCCGCA CGGAGCTTCA GTTGCCTGGC TGCCCCACCC CCGTGTCTTT CGGCCTCCTC
    TTTTCTGTGG CCTTCCCCGT GGATGGAGAG CCTGATGCAG AGGTTGTGGT GGGAACCACG
    AGGCCAGAGA CGCTGCCTGG AGACGTGGCT GTGGCGGTCC ATCCTGATGA TTCCCGCTAC
    ACGCATCTAC ATGGGCGACA ACTCTGTCAT CCCTTGACGG GGCAGCTTCT CCCTCTTATC
    ACAGACTGCA CTGTTCAGCC ACACCTGGGC ACAGGGGCGG TGAAGGTGAC TCCAGCGCAC
    AGCCCTGCCG ATGCTGAGCT GGGGGCCCGA CACGGCCTGA GCCCCCGGAG TGTCATTGCG
    GAGGATGGGA CCATGACCGC CCTTTGTGAG GACTGGCTGC AGGGTCTTCA CCGATTTGTG
    GCCCGGGAAA AGATTCTGTC AGCACTGAGG GAGCGGGGCC TGTTCCGGGG CCTCCAGAAC
    CACCCCATGG TGCTGCCCAT TTGCAGCCGT TCCGGGGACG TGGTAGAGTA CCTACTGAAG
    AGCCAGTGGT TTGTCCGCTG CCGGGAAATG GGGGAGCGAG CCGCCAAGGC CGTGGAGTCG
    GGGGCCCTGG ACCTCAGTCC CTCCTTCCAC CAGAAGAACT GGCAGCACTG GTTTTCCCAC
    ATTGGGGACT GGTGTGTCTC CCGGCAGCTG TGGTGGGGCC ATCGGATTCC AGCCTACCTG
    GTTGTAGAGG AACATGCAAA GGGTGAAACG GAGGACTTCT GGGTGGTCGG GCGGACAGAG
    GCTGAGGCCA GAGAAGTAGC TGCAGAATTA ACAGGGAGAC CAGGGGCGGA GCTGACCCTG
    GAGAGGGACC CTGATGTCCT GGACACGTGG TTCTCCTCCG CTCTTTTCCC CTTTTCTGCC
    CTGGGCTGGC CCCGAGAGAC CCCGGACCTG GCTCGTTTCT ACCCCCTGTC ACTTTTGGAA
    ACGGGCAGTG ACCTCCTGCT CTTCTGGGTG GGCCGCATGG TCATGTTGGG GACCCAGCTC
    ACAGGGCAGC TCCCCTTCAG CAAGGTCCTG CTTCACTCCA TGGTCCGGGA CAGGCAGGGC
    CGGAAGATGA GCAAGTCCCT GGGGAACGTG CTGGACCCAC GGGACATCAT CAGTGGGGTG
    GAGCTGCAGG TACTGCAGGA CAATCTGAGG GATGGAAACT TGGACCCCGC AGAGCTGGCG
    ATTGCAGCCT CAGCGCAGAG AAAGGACTTC CCTCATGGGA TCCCCGAGTG TGGGACAGAT
    GCTCTGAGGT TCACCCTGTG CTCCCATGGG GCCCTGGGAG GTGACTTGCA CCTGTCCGTC
    TCTGAGGTCC TGAGCTTCCG ACACTTCTGC AATAAGATCT GGAATGCCCT GCGCTTTATC
    CTGAATGCTC TTGGGGAGGA ATTCATACCC CAACCTGCGG AGGAGCTGTC CCCCGCCTCC
    CGCATGGACA CCTGGATCCT CAGCTGCCTG GCCCGCACGG CCCAGGACTG CGAGCGGGGC
    TTCCTCACCC GCGAGCTCTC GCTCGTCACC CACGCCCTGC ACCACTTCTG GCTGCACAAC
    CTCTGTGATG TCTACTTGGA GGCTGTGAAG CCGGTGCTAT CACACTCTCC CCGCCCTCTG
    GAGCCGCCTC AGGTCCTGTT CTTCTGCGCA GACGTCGGTC TCCGCCTCCT CGCCCCGTTG
    ATGCCTTTCC TGGCTGAAGA GCTCTGGCAG AGGCTGCCCC CCAGGCCTGG TGGTCCCTCT
    GCCCCCAGCA TCTGTGTTGC CCCCTACCCA AGTGCCCGCA GCTTGGAGCA CTGGCACCAG
    CCTGAGCTGG AGAGGCGCTT CTCCCGGGTT CAGGAGTCCG TGCAGGTGCT CAGGGCTCTG
    CGCGCCACCT ACCAGCTCAC CAAGGCCCGG CCCCGAGTGC TGCTGCAGAG CTCAGAGCCA
    GGCGAGCAGG GCCTCTTTGA GGCCTTCCTG GAGCCGCTGG GCATCCTGGG CTACTGTGGG
    GCGGTGGGCC TTCTACCCCC TGGTGCAGCA GCCCCCTCAG GCTGGGTCCA GGCCGCCCTC
    AGTGACACCA GTCAGGCGTA TATGGAGCTG CAGGGCCTGG TGGACCCCCA GACCCACCTA
    CCTCGGCTGG CTGCCCGAAG ACACAAGTTG CAGAAGCAGC TTGATGGCCT CCTAGCCCGG
    CCCCCATCCG AGGGACAGGC GGAGACTCAG AGGCAGCAGA GGCTTTCTTC CCTCCAGTTG
    GAGTTGTCAA AACTGGACAA GGCGGCCTCT CACCTCCAGC AGCTGATGGA TGATTTTCCA
    AGCCCTGGGA GCTCACCATC CCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010816612.1
    CDS50..2896
    Translation

    Target ORF information:

    RefSeq Version XM_010818310.2
    Organism Bos taurus(cattle)
    Definition Bos taurus valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010818310.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAAATCCC CACGGAACAT GGAGAAAAGA AAGGCCAGGC TGCCCCAGGC CACAGGGGAG 
    ACCTTTTCCA TGTGTATCCC ACCCCCTAAT GTCACAGGCT CCCTGCACAT TGGGCATGCG
    CTGACCGTGG CCATACAGGA TGCTCTCGTG CGCTGGCACC GGATGCGTGG GGATCGGGTG
    CTGTGGGTCC CTGGCTCAGA TCATGCAGGG ATTGCAACCC AAGCTGTGGT GGAGAAACAG
    CTGTGGAAGG AGCGAGGCGT GAGGAGACAT GAGCTGAGCC GGGAAGACTT CCTTAGGGAG
    GTGTGGAAGT GGAAGGAGGA GAAAGGTGGC GAGATCTGTG AGCAGCTCCG AGCGCTGGGG
    GCCTCCCTGG ACTGGGACCG AGAGTGCTTT ACCATGGATA CCGGCTCCTC AGTGGCCGTG
    ACAGAAGCTT TCGTGCGGCT CTACAAGGCT GGGTTGTTGT ACCGGAGCCG GCAGCTGGTC
    AATTGGTCGT GTGCCTTACG CTCCGCCATC TCGGATATTG AGGTGGAGAG CCGGCCCCTC
    CCTGGCCGCA CGGAGCTTCA GTTGCCTGGC TGCCCCACCC CCGTGTCTTT CGGCCTCCTC
    TTTTCTGTGG CCTTCCCCGT GGATGGAGAG CCTGATGCAG AGGTTGTGGT GGGAACCACG
    AGGCCAGAGA CGCTGCCTGG AGACGTGGCT GTGGCGGTCC ATCCTGATGA TTCCCGCTAC
    ACGCATCTAC ATGGGCGACA ACTCTGTCAT CCCTTGACGG GGCAGCTTCT CCCTCTTATC
    ACAGACTGCA CTGTTCAGCC ACACCTGGGC ACAGGGGCGG TGAAGGTGAC TCCAGCGCAC
    AGCCCTGCCG ATGCTGAGCT GGGGGCCCGA CACGGCCTGA GCCCCCGGAG TGTCATTGCG
    GAGGATGGGA CCATGACCGC CCTTTGTGAG GACTGGCTGC AGGGTCTTCA CCGATTTGTG
    GCCCGGGAAA AGATTCTGTC AGCACTGAGG GAGCGGGGCC TGTTCCGGGG CCTCCAGAAC
    CACCCCATGG TGCTGCCCAT TTGCAGCCGT TCCGGGGACG TGGTAGAGTA CCTACTGAAG
    AGCCAGTGGT TTGTCCGCTG CCGGGAAATG GGGGAGCGAG CCGCCAAGGC CGTGGAGTCG
    GGGGCCCTGG ACCTCAGTCC CTCCTTCCAC CAGAAGAACT GGCAGCACTG GTTTTCCCAC
    ATTGGGGACT GGTGTGTCTC CCGGCAGCTG TGGTGGGGCC ATCGGATTCC AGCCTACCTG
    GTTGTAGAGG AACATGCAAA GGGTGAAACG GAGGACTTCT GGGTGGTCGG GCGGACAGAG
    GCTGAGGCCA GAGAAGTAGC TGCAGAATTA ACAGGGAGAC CAGGGGCGGA GCTGACCCTG
    GAGAGGGACC CTGATGTCCT GGACACGTGG TTCTCCTCCG CTCTTTTCCC CTTTTCTGCC
    CTGGGCTGGC CCCGAGAGAC CCCGGACCTG GCTCGTTTCT ACCCCCTGTC ACTTTTGGAA
    ACGGGCAGTG ACCTCCTGCT CTTCTGGGTG GGCCGCATGG TCATGTTGGG GACCCAGCTC
    ACAGGGCAGC TCCCCTTCAG CAAGGTCCTG CTTCACTCCA TGGTCCGGGA CAGGCAGGGC
    CGGAAGATGA GCAAGTCCCT GGGGAACGTG CTGGACCCAC GGGACATCAT CAGTGGGGTG
    GAGCTGCAGG TACTGCAGGA CAATCTGAGG GATGGAAACT TGGACCCCGC AGAGCTGGCG
    ATTGCAGCCT CAGCGCAGAG AAAGGACTTC CCTCATGGGA TCCCCGAGTG TGGGACAGAT
    GCTCTGAGGT TCACCCTGTG CTCCCATGGG GCCCTGGGAG GTGACTTGCA CCTGTCCGTC
    TCTGAGGTCC TGAGCTTCCG ACACTTCTGC AATAAGATCT GGAATGCCCT GCGCTTTATC
    CTGAATGCTC TTGGGGAGGA ATTCATACCC CAACCTGCGG AGGAGCTGTC CCCCGCCTCC
    CGCATGGACA CCTGGATCCT CAGCTGCCTG GCCCGCACGG CCCAGGACTG CGAGCGGGGC
    TTCCTCACCC GCGAGCTCTC GCTCGTCACC CACGCCCTGC ACCACTTCTG GCTGCACAAC
    CTCTGTGATG TCTACTTGGA GGCTGTGAAG CCGGTGCTAT CACACTCTCC CCGCCCTCTG
    GAGCCGCCTC AGGTCCTGTT CTTCTGCGCA GACGTCGGTC TCCGCCTCCT CGCCCCGTTG
    ATGCCTTTCC TGGCTGAAGA GCTCTGGCAG AGGCTGCCCC CCAGGCCTGG TGGTCCCTCT
    GCCCCCAGCA TCTGTGTTGC CCCCTACCCA AGTGCCCGCA GCTTGGAGCA CTGGCACCAG
    CCTGAGCTGG AGAGGCGCTT CTCCCGGGTT CAGGAGTCCG TGCAGGTGCT CAGGGCTCTG
    CGCGCCACCT ACCAGCTCAC CAAGGCCCGG CCCCGAGTGC TGCTGCAGAG CTCAGAGCCA
    GGCGAGCAGG GCCTCTTTGA GGCCTTCCTG GAGCCGCTGG GCATCCTGGG CTACTGTGGG
    GCGGTGGGCC TTCTACCCCC TGGTGCAGCA GCCCCCTCAG GCTGGGTCCA GGCCGCCCTC
    AGTGACACCA GTCAGGCGTA TATGGAGCTG CAGGGCCTGG TGGACCCCCA GACCCACCTA
    CCTCGGCTGG CTGCCCGAAG ACACAAGTTG CAGAAGCAGC TTGATGGCCT CCTAGCCCGG
    CCCCCATCCG AGGGACAGGC GGAGACTCAG AGGCAGCAGA GGCTTTCTTC CCTCCAGTTG
    GAGTTGTCAA AACTGGACAA GGCGGCCTCT CACCTCCAGC AGCTGATGGA TGATTTTCCA
    AGCCCTGGGA GCTCACCATC CCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa60390
    Clone ID Related Accession (Same CDS sequence) XM_005223583.3 , XM_005223583.4
    Accession Version XM_005223583.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2934bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product valine--tRNA ligase, mitochondrial isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104557.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005223583.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGCCTCATT TACCTCTGAC CTCTTTTCGA CCACCGCTTT GGGGCTTGAG GCCCTCACGG 
    GGCCTCCCCA GGTCCCGTCC CCTTTCCACC CAGTCAGAGC CAAATGGATC AGCCATCTCC
    CGGAGGAACC GTGAAGCCAA ACAGAAGCGC CTGCGAGAGA AGCAGGCATC GCTGGAGGCT
    GGGATAGCCC CCAAGAGCAA GTCACCTGCA GAATCCAGTA AGGCCTGGAC CCCTAAGGAG
    AGAGTGTTGT ATGAAATCCC CACGGAACAT GGAGAAAAGA AAGATGTCTC CCGACCCCTG
    CCTCCTGCAT ACAGCCCCCA GTACGTTGAG GCTGCCTGGT ACCCTTGGTG GGTGCGAGAG
    GGCTTCTTCA AACCAGAATA TCAGGCCAGG CTGCCCCAGG CCACAGGGGA GACCTTTTCC
    ATGTGTATCC CACCCCCTAA TGTCACAGGC TCCCTGCACA TTGGGCATGC GCTGACCGTG
    GCCATACAGG ATGCTCTCGT GCGCTGGCAC CGGATGCGTG GGGATCGGGT GCTGTGGGTC
    CCTGGCTCAG ATCATGCAGG GATTGCAACC CAAGCTGTGG TGGAGAAACA GCTGTGGAAG
    GAGCGAGGCG TGAGGAGACA TGAGCTGAGC CGGGAAGACT TCCTTAGGGA GGTGTGGAAG
    TGGAAGGAGG AGAAAGGTGG CGAGATCTGT GAGCAGCTCC GAGCGCTGGG GGCCTCCCTG
    GACTGGGACC GAGAGTGCTT TACCATGGAT ACCGGCTCCT CAGTGGCCGT GACAGAAGCT
    TTCGTGCGGC TCTACAAGGC TGGGTTGTTG TACCGGAGCC GGCAGCTGGT CAATTGGTCG
    TGTGCCTTAC GCTCCGCCAT CTCGGATATT GAGGTGGAGA GCCGGCCCCT CCCTGGCCGC
    ACGGAGCTTC AGTTGCCTGG CTGCCCCACC CCCGTGTCTT TCGGCCTCCT CTTTTCTGTG
    GCCTTCCCCG TGGATGGAGA GCCTGATGCA GAGGTTGTGG TGGGAACCAC GAGGCCAGAG
    ACGCTGCCTG GAGACGTGGC TGTGGCGGTC CATCCTGATG ATTCCCGCTA CACGCATCTA
    CATGGGCGAC AACTCTGTCA TCCCTTGACG GGGCAGCTTC TCCCTCTTAT CACAGACTGC
    ACTGTTCAGC CACACCTGGG CACAGGGGCG GTGAAGGTGA CTCCAGCGCA CAGCCCTGCC
    GATGCTGAGC TGGGGGCCCG ACACGGCCTG AGCCCCCGGA GTGTCATTGC GGAGGATGGG
    ACCATGACCG CCCTTTGTGA GGACTGGCTG CAGGGTCTTC ACCGATTTGT GGCCCGGGAA
    AAGATTCTGT CAGCACTGAG GGAGCGGGGC CTGTTCCGGG GCCTCCAGAA CCACCCCATG
    GTGCTGCCCA TTTGCAGCCG TTCCGGGGAC GTGGTAGAGT ACCTACTGAA GAGCCAGTGG
    TTTGTCCGCT GCCGGGAAAT GGGGGAGCGA GCCGCCAAGG CCGTGGAGTC GGGGGCCCTG
    GACCTCAGTC CCTCCTTCCA CCAGAAGAAC TGGCAGCACT GGTTTTCCCA CATTGGGGAC
    TGGTGTGTCT CCCGGCAGCT GTGGTGGGGC CATCGGATTC CAGCCTACCT GGTTGTAGAG
    GAACATGCAA AGGGTGAAAC GGAGGACTTC TGGGTGGTCG GGCGGACAGA GGCTGAGGCC
    AGAGAAGTAG CTGCAGAATT AACAGGGAGA CCAGGGGCGG AGCTGACCCT GGAGAGGGAC
    CCTGATGTCC TGGACACGTG GTTCTCCTCC GCTCTTTTCC CCTTTTCTGC CCTGGGCTGG
    CCCCGAGAGA CCCCGGACCT GGCTCGTTTC TACCCCCTGT CACTTTTGGA AACGGGCAGT
    GACCTCCTGC TCTTCTGGGT GGGCCGCATG GTCATGTTGG GGACCCAGCT CACAGGGCAG
    CTCCCCTTCA GCAAGGTCCT GCTTCACTCC ATGGTCCGGG ACAGGCAGGG CCGGAAGATG
    AGCAAGTCCC TGGGGAACGT GCTGGACCCA CGGGACATCA TCAGTGGGGT GGAGCTGCAG
    GTACTGCAGG ACAATCTGAG GGATGGAAAC TTGGACCCCG CAGAGCTGGC GATTGCAGCC
    TCAGCGCAGA GAAAGGACTT CCCTCATGGG ATCCCCGAGT GTGGGACAGA TGCTCTGAGG
    TTCACCCTGT GCTCCCATGG GGCCCTGGGA GGTGACTTGC ACCTGTCCGT CTCTGAGGTC
    CTGAGCTTCC GACACTTCTG CAATAAGATC TGGAATGCCC TGCGCTTTAT CCTGAATGCT
    CTTGGGGAGG AATTCATACC CCAACCTGCG GAGGAGCTGT CCCCCGCCTC CCGCATGGAC
    ACCTGGATCC TCAGCTGCCT GGCCCGCACG GCCCAGGACT GCGAGCGGGG CTTCCTCACC
    CGCGAGCTCT CGCTCGTCAC CCACGCCCTG CACCACTTCT GGCTGCACAA CCTCTGTGAT
    GTCTACTTGG AGGCTGTGAA GCCGGTGCTA TCACACTCTC CCCGCCCTCT GGAGCCGCCT
    CAGGTCCTGT TCTTCTGCGC AGACGTCGGT CTCCGCCTCC TCGCCCCGTT GATGCCTTTC
    CTGGCTGAAG AGCTCTGGCA GAGGCTGCCC CCCAGGCCTG GTGGTCCCTC TGCCCCCAGC
    ATCTGTGTTG CCCCCTACCC AAGTGCCCGC AGCTTGTGCT GCTGCAGAGC TCAGAGCCAG
    GCGAGCAGGG CCTCTTTGAG GCCTTCCTGG AGCCGCTGGG CATCCTGGGC TACTGTGGGG
    CGGTGGGCCT TCTACCCCCT GGTGCAGCAG CCCCCTCAGG CTGGGTCCAG GCCGCCCTCA
    GTGACACCAG TCAGGCGTAT ATGGAGCTGC AGGGCCTGGT GGACCCCCAG ACCCACCTAC
    CTCGGCTGGC TGCCCGAAGA CACAAGTTGC AGAAGCAGCT TGATGGCCTC CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005223640.2
    CDS231..3164
    Translation

    Target ORF information:

    RefSeq Version XM_005223583.3
    Organism Bos taurus(cattle)
    Definition Bos taurus valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005223583.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGCCTCATT TACCTCTGAC CTCTTTTCGA CCACCGCTTT GGGGCTTGAG GCCCTCACGG 
    GGCCTCCCCA GGTCCCGTCC CCTTTCCACC CAGTCAGAGC CAAATGGATC AGCCATCTCC
    CGGAGGAACC GTGAAGCCAA ACAGAAGCGC CTGCGAGAGA AGCAGGCATC GCTGGAGGCT
    GGGATAGCCC CCAAGAGCAA GTCACCTGCA GAATCCAGTA AGGCCTGGAC CCCTAAGGAG
    AGAGTGTTGT ATGAAATCCC CACGGAACAT GGAGAAAAGA AAGATGTCTC CCGACCCCTG
    CCTCCTGCAT ACAGCCCCCA GTACGTTGAG GCTGCCTGGT ACCCTTGGTG GGTGCGAGAG
    GGCTTCTTCA AACCAGAATA TCAGGCCAGG CTGCCCCAGG CCACAGGGGA GACCTTTTCC
    ATGTGTATCC CACCCCCTAA TGTCACAGGC TCCCTGCACA TTGGGCATGC GCTGACCGTG
    GCCATACAGG ATGCTCTCGT GCGCTGGCAC CGGATGCGTG GGGATCGGGT GCTGTGGGTC
    CCTGGCTCAG ATCATGCAGG GATTGCAACC CAAGCTGTGG TGGAGAAACA GCTGTGGAAG
    GAGCGAGGCG TGAGGAGACA TGAGCTGAGC CGGGAAGACT TCCTTAGGGA GGTGTGGAAG
    TGGAAGGAGG AGAAAGGTGG CGAGATCTGT GAGCAGCTCC GAGCGCTGGG GGCCTCCCTG
    GACTGGGACC GAGAGTGCTT TACCATGGAT ACCGGCTCCT CAGTGGCCGT GACAGAAGCT
    TTCGTGCGGC TCTACAAGGC TGGGTTGTTG TACCGGAGCC GGCAGCTGGT CAATTGGTCG
    TGTGCCTTAC GCTCCGCCAT CTCGGATATT GAGGTGGAGA GCCGGCCCCT CCCTGGCCGC
    ACGGAGCTTC AGTTGCCTGG CTGCCCCACC CCCGTGTCTT TCGGCCTCCT CTTTTCTGTG
    GCCTTCCCCG TGGATGGAGA GCCTGATGCA GAGGTTGTGG TGGGAACCAC GAGGCCAGAG
    ACGCTGCCTG GAGACGTGGC TGTGGCGGTC CATCCTGATG ATTCCCGCTA CACGCATCTA
    CATGGGCGAC AACTCTGTCA TCCCTTGACG GGGCAGCTTC TCCCTCTTAT CACAGACTGC
    ACTGTTCAGC CACACCTGGG CACAGGGGCG GTGAAGGTGA CTCCAGCGCA CAGCCCTGCC
    GATGCTGAGC TGGGGGCCCG ACACGGCCTG AGCCCCCGGA GTGTCATTGC GGAGGATGGG
    ACCATGACCG CCCTTTGTGA GGACTGGCTG CAGGGTCTTC ACCGATTTGT GGCCCGGGAA
    AAGATTCTGT CAGCACTGAG GGAGCGGGGC CTGTTCCGGG GCCTCCAGAA CCACCCCATG
    GTGCTGCCCA TTTGCAGCCG TTCCGGGGAC GTGGTAGAGT ACCTACTGAA GAGCCAGTGG
    TTTGTCCGCT GCCGGGAAAT GGGGGAGCGA GCCGCCAAGG CCGTGGAGTC GGGGGCCCTG
    GACCTCAGTC CCTCCTTCCA CCAGAAGAAC TGGCAGCACT GGTTTTCCCA CATTGGGGAC
    TGGTGTGTCT CCCGGCAGCT GTGGTGGGGC CATCGGATTC CAGCCTACCT GGTTGTAGAG
    GAACATGCAA AGGGTGAAAC GGAGGACTTC TGGGTGGTCG GGCGGACAGA GGCTGAGGCC
    AGAGAAGTAG CTGCAGAATT AACAGGGAGA CCAGGGGCGG AGCTGACCCT GGAGAGGGAC
    CCTGATGTCC TGGACACGTG GTTCTCCTCC GCTCTTTTCC CCTTTTCTGC CCTGGGCTGG
    CCCCGAGAGA CCCCGGACCT GGCTCGTTTC TACCCCCTGT CACTTTTGGA AACGGGCAGT
    GACCTCCTGC TCTTCTGGGT GGGCCGCATG GTCATGTTGG GGACCCAGCT CACAGGGCAG
    CTCCCCTTCA GCAAGGTCCT GCTTCACTCC ATGGTCCGGG ACAGGCAGGG CCGGAAGATG
    AGCAAGTCCC TGGGGAACGT GCTGGACCCA CGGGACATCA TCAGTGGGGT GGAGCTGCAG
    GTACTGCAGG ACAATCTGAG GGATGGAAAC TTGGACCCCG CAGAGCTGGC GATTGCAGCC
    TCAGCGCAGA GAAAGGACTT CCCTCATGGG ATCCCCGAGT GTGGGACAGA TGCTCTGAGG
    TTCACCCTGT GCTCCCATGG GGCCCTGGGA GGTGACTTGC ACCTGTCCGT CTCTGAGGTC
    CTGAGCTTCC GACACTTCTG CAATAAGATC TGGAATGCCC TGCGCTTTAT CCTGAATGCT
    CTTGGGGAGG AATTCATACC CCAACCTGCG GAGGAGCTGT CCCCCGCCTC CCGCATGGAC
    ACCTGGATCC TCAGCTGCCT GGCCCGCACG GCCCAGGACT GCGAGCGGGG CTTCCTCACC
    CGCGAGCTCT CGCTCGTCAC CCACGCCCTG CACCACTTCT GGCTGCACAA CCTCTGTGAT
    GTCTACTTGG AGGCTGTGAA GCCGGTGCTA TCACACTCTC CCCGCCCTCT GGAGCCGCCT
    CAGGTCCTGT TCTTCTGCGC AGACGTCGGT CTCCGCCTCC TCGCCCCGTT GATGCCTTTC
    CTGGCTGAAG AGCTCTGGCA GAGGCTGCCC CCCAGGCCTG GTGGTCCCTC TGCCCCCAGC
    ATCTGTGTTG CCCCCTACCC AAGTGCCCGC AGCTTGTGCT GCTGCAGAGC TCAGAGCCAG
    GCGAGCAGGG CCTCTTTGAG GCCTTCCTGG AGCCGCTGGG CATCCTGGGC TACTGTGGGG
    CGGTGGGCCT TCTACCCCCT GGTGCAGCAG CCCCCTCAGG CTGGGTCCAG GCCGCCCTCA
    GTGACACCAG TCAGGCGTAT ATGGAGCTGC AGGGCCTGGT GGACCCCCAG ACCCACCTAC
    CTCGGCTGGC TGCCCGAAGA CACAAGTTGC AGAAGCAGCT TGATGGCCTC CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa60390
    Clone ID Related Accession (Same CDS sequence) XM_005223583.3 , XM_005223583.4
    Accession Version XM_005223583.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2934bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product valine--tRNA ligase, mitochondrial isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005223583.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGCCTCATT TACCTCTGAC CTCTTTTCGA CCACCGCTTT GGGGCTTGAG GCCCTCACGG 
    GGCCTCCCCA GGTCCCGTCC CCTTTCCACC CAGTCAGAGC CAAATGGATC AGCCATCTCC
    CGGAGGAACC GTGAAGCCAA ACAGAAGCGC CTGCGAGAGA AGCAGGCATC GCTGGAGGCT
    GGGATAGCCC CCAAGAGCAA GTCACCTGCA GAATCCAGTA AGGCCTGGAC CCCTAAGGAG
    AGAGTGTTGT ATGAAATCCC CACGGAACAT GGAGAAAAGA AAGATGTCTC CCGACCCCTG
    CCTCCTGCAT ACAGCCCCCA GTACGTTGAG GCTGCCTGGT ACCCTTGGTG GGTGCGAGAG
    GGCTTCTTCA AACCAGAATA TCAGGCCAGG CTGCCCCAGG CCACAGGGGA GACCTTTTCC
    ATGTGTATCC CACCCCCTAA TGTCACAGGC TCCCTGCACA TTGGGCATGC GCTGACCGTG
    GCCATACAGG ATGCTCTCGT GCGCTGGCAC CGGATGCGTG GGGATCGGGT GCTGTGGGTC
    CCTGGCTCAG ATCATGCAGG GATTGCAACC CAAGCTGTGG TGGAGAAACA GCTGTGGAAG
    GAGCGAGGCG TGAGGAGACA TGAGCTGAGC CGGGAAGACT TCCTTAGGGA GGTGTGGAAG
    TGGAAGGAGG AGAAAGGTGG CGAGATCTGT GAGCAGCTCC GAGCGCTGGG GGCCTCCCTG
    GACTGGGACC GAGAGTGCTT TACCATGGAT ACCGGCTCCT CAGTGGCCGT GACAGAAGCT
    TTCGTGCGGC TCTACAAGGC TGGGTTGTTG TACCGGAGCC GGCAGCTGGT CAATTGGTCG
    TGTGCCTTAC GCTCCGCCAT CTCGGATATT GAGGTGGAGA GCCGGCCCCT CCCTGGCCGC
    ACGGAGCTTC AGTTGCCTGG CTGCCCCACC CCCGTGTCTT TCGGCCTCCT CTTTTCTGTG
    GCCTTCCCCG TGGATGGAGA GCCTGATGCA GAGGTTGTGG TGGGAACCAC GAGGCCAGAG
    ACGCTGCCTG GAGACGTGGC TGTGGCGGTC CATCCTGATG ATTCCCGCTA CACGCATCTA
    CATGGGCGAC AACTCTGTCA TCCCTTGACG GGGCAGCTTC TCCCTCTTAT CACAGACTGC
    ACTGTTCAGC CACACCTGGG CACAGGGGCG GTGAAGGTGA CTCCAGCGCA CAGCCCTGCC
    GATGCTGAGC TGGGGGCCCG ACACGGCCTG AGCCCCCGGA GTGTCATTGC GGAGGATGGG
    ACCATGACCG CCCTTTGTGA GGACTGGCTG CAGGGTCTTC ACCGATTTGT GGCCCGGGAA
    AAGATTCTGT CAGCACTGAG GGAGCGGGGC CTGTTCCGGG GCCTCCAGAA CCACCCCATG
    GTGCTGCCCA TTTGCAGCCG TTCCGGGGAC GTGGTAGAGT ACCTACTGAA GAGCCAGTGG
    TTTGTCCGCT GCCGGGAAAT GGGGGAGCGA GCCGCCAAGG CCGTGGAGTC GGGGGCCCTG
    GACCTCAGTC CCTCCTTCCA CCAGAAGAAC TGGCAGCACT GGTTTTCCCA CATTGGGGAC
    TGGTGTGTCT CCCGGCAGCT GTGGTGGGGC CATCGGATTC CAGCCTACCT GGTTGTAGAG
    GAACATGCAA AGGGTGAAAC GGAGGACTTC TGGGTGGTCG GGCGGACAGA GGCTGAGGCC
    AGAGAAGTAG CTGCAGAATT AACAGGGAGA CCAGGGGCGG AGCTGACCCT GGAGAGGGAC
    CCTGATGTCC TGGACACGTG GTTCTCCTCC GCTCTTTTCC CCTTTTCTGC CCTGGGCTGG
    CCCCGAGAGA CCCCGGACCT GGCTCGTTTC TACCCCCTGT CACTTTTGGA AACGGGCAGT
    GACCTCCTGC TCTTCTGGGT GGGCCGCATG GTCATGTTGG GGACCCAGCT CACAGGGCAG
    CTCCCCTTCA GCAAGGTCCT GCTTCACTCC ATGGTCCGGG ACAGGCAGGG CCGGAAGATG
    AGCAAGTCCC TGGGGAACGT GCTGGACCCA CGGGACATCA TCAGTGGGGT GGAGCTGCAG
    GTACTGCAGG ACAATCTGAG GGATGGAAAC TTGGACCCCG CAGAGCTGGC GATTGCAGCC
    TCAGCGCAGA GAAAGGACTT CCCTCATGGG ATCCCCGAGT GTGGGACAGA TGCTCTGAGG
    TTCACCCTGT GCTCCCATGG GGCCCTGGGA GGTGACTTGC ACCTGTCCGT CTCTGAGGTC
    CTGAGCTTCC GACACTTCTG CAATAAGATC TGGAATGCCC TGCGCTTTAT CCTGAATGCT
    CTTGGGGAGG AATTCATACC CCAACCTGCG GAGGAGCTGT CCCCCGCCTC CCGCATGGAC
    ACCTGGATCC TCAGCTGCCT GGCCCGCACG GCCCAGGACT GCGAGCGGGG CTTCCTCACC
    CGCGAGCTCT CGCTCGTCAC CCACGCCCTG CACCACTTCT GGCTGCACAA CCTCTGTGAT
    GTCTACTTGG AGGCTGTGAA GCCGGTGCTA TCACACTCTC CCCGCCCTCT GGAGCCGCCT
    CAGGTCCTGT TCTTCTGCGC AGACGTCGGT CTCCGCCTCC TCGCCCCGTT GATGCCTTTC
    CTGGCTGAAG AGCTCTGGCA GAGGCTGCCC CCCAGGCCTG GTGGTCCCTC TGCCCCCAGC
    ATCTGTGTTG CCCCCTACCC AAGTGCCCGC AGCTTGTGCT GCTGCAGAGC TCAGAGCCAG
    GCGAGCAGGG CCTCTTTGAG GCCTTCCTGG AGCCGCTGGG CATCCTGGGC TACTGTGGGG
    CGGTGGGCCT TCTACCCCCT GGTGCAGCAG CCCCCTCAGG CTGGGTCCAG GCCGCCCTCA
    GTGACACCAG TCAGGCGTAT ATGGAGCTGC AGGGCCTGGT GGACCCCCAG ACCCACCTAC
    CTCGGCTGGC TGCCCGAAGA CACAAGTTGC AGAAGCAGCT TGATGGCCTC CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005223640.2
    CDS224..3157
    Translation

    Target ORF information:

    RefSeq Version XM_005223583.4
    Organism Bos taurus(cattle)
    Definition Bos taurus valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005223583.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGCCTCATT TACCTCTGAC CTCTTTTCGA CCACCGCTTT GGGGCTTGAG GCCCTCACGG 
    GGCCTCCCCA GGTCCCGTCC CCTTTCCACC CAGTCAGAGC CAAATGGATC AGCCATCTCC
    CGGAGGAACC GTGAAGCCAA ACAGAAGCGC CTGCGAGAGA AGCAGGCATC GCTGGAGGCT
    GGGATAGCCC CCAAGAGCAA GTCACCTGCA GAATCCAGTA AGGCCTGGAC CCCTAAGGAG
    AGAGTGTTGT ATGAAATCCC CACGGAACAT GGAGAAAAGA AAGATGTCTC CCGACCCCTG
    CCTCCTGCAT ACAGCCCCCA GTACGTTGAG GCTGCCTGGT ACCCTTGGTG GGTGCGAGAG
    GGCTTCTTCA AACCAGAATA TCAGGCCAGG CTGCCCCAGG CCACAGGGGA GACCTTTTCC
    ATGTGTATCC CACCCCCTAA TGTCACAGGC TCCCTGCACA TTGGGCATGC GCTGACCGTG
    GCCATACAGG ATGCTCTCGT GCGCTGGCAC CGGATGCGTG GGGATCGGGT GCTGTGGGTC
    CCTGGCTCAG ATCATGCAGG GATTGCAACC CAAGCTGTGG TGGAGAAACA GCTGTGGAAG
    GAGCGAGGCG TGAGGAGACA TGAGCTGAGC CGGGAAGACT TCCTTAGGGA GGTGTGGAAG
    TGGAAGGAGG AGAAAGGTGG CGAGATCTGT GAGCAGCTCC GAGCGCTGGG GGCCTCCCTG
    GACTGGGACC GAGAGTGCTT TACCATGGAT ACCGGCTCCT CAGTGGCCGT GACAGAAGCT
    TTCGTGCGGC TCTACAAGGC TGGGTTGTTG TACCGGAGCC GGCAGCTGGT CAATTGGTCG
    TGTGCCTTAC GCTCCGCCAT CTCGGATATT GAGGTGGAGA GCCGGCCCCT CCCTGGCCGC
    ACGGAGCTTC AGTTGCCTGG CTGCCCCACC CCCGTGTCTT TCGGCCTCCT CTTTTCTGTG
    GCCTTCCCCG TGGATGGAGA GCCTGATGCA GAGGTTGTGG TGGGAACCAC GAGGCCAGAG
    ACGCTGCCTG GAGACGTGGC TGTGGCGGTC CATCCTGATG ATTCCCGCTA CACGCATCTA
    CATGGGCGAC AACTCTGTCA TCCCTTGACG GGGCAGCTTC TCCCTCTTAT CACAGACTGC
    ACTGTTCAGC CACACCTGGG CACAGGGGCG GTGAAGGTGA CTCCAGCGCA CAGCCCTGCC
    GATGCTGAGC TGGGGGCCCG ACACGGCCTG AGCCCCCGGA GTGTCATTGC GGAGGATGGG
    ACCATGACCG CCCTTTGTGA GGACTGGCTG CAGGGTCTTC ACCGATTTGT GGCCCGGGAA
    AAGATTCTGT CAGCACTGAG GGAGCGGGGC CTGTTCCGGG GCCTCCAGAA CCACCCCATG
    GTGCTGCCCA TTTGCAGCCG TTCCGGGGAC GTGGTAGAGT ACCTACTGAA GAGCCAGTGG
    TTTGTCCGCT GCCGGGAAAT GGGGGAGCGA GCCGCCAAGG CCGTGGAGTC GGGGGCCCTG
    GACCTCAGTC CCTCCTTCCA CCAGAAGAAC TGGCAGCACT GGTTTTCCCA CATTGGGGAC
    TGGTGTGTCT CCCGGCAGCT GTGGTGGGGC CATCGGATTC CAGCCTACCT GGTTGTAGAG
    GAACATGCAA AGGGTGAAAC GGAGGACTTC TGGGTGGTCG GGCGGACAGA GGCTGAGGCC
    AGAGAAGTAG CTGCAGAATT AACAGGGAGA CCAGGGGCGG AGCTGACCCT GGAGAGGGAC
    CCTGATGTCC TGGACACGTG GTTCTCCTCC GCTCTTTTCC CCTTTTCTGC CCTGGGCTGG
    CCCCGAGAGA CCCCGGACCT GGCTCGTTTC TACCCCCTGT CACTTTTGGA AACGGGCAGT
    GACCTCCTGC TCTTCTGGGT GGGCCGCATG GTCATGTTGG GGACCCAGCT CACAGGGCAG
    CTCCCCTTCA GCAAGGTCCT GCTTCACTCC ATGGTCCGGG ACAGGCAGGG CCGGAAGATG
    AGCAAGTCCC TGGGGAACGT GCTGGACCCA CGGGACATCA TCAGTGGGGT GGAGCTGCAG
    GTACTGCAGG ACAATCTGAG GGATGGAAAC TTGGACCCCG CAGAGCTGGC GATTGCAGCC
    TCAGCGCAGA GAAAGGACTT CCCTCATGGG ATCCCCGAGT GTGGGACAGA TGCTCTGAGG
    TTCACCCTGT GCTCCCATGG GGCCCTGGGA GGTGACTTGC ACCTGTCCGT CTCTGAGGTC
    CTGAGCTTCC GACACTTCTG CAATAAGATC TGGAATGCCC TGCGCTTTAT CCTGAATGCT
    CTTGGGGAGG AATTCATACC CCAACCTGCG GAGGAGCTGT CCCCCGCCTC CCGCATGGAC
    ACCTGGATCC TCAGCTGCCT GGCCCGCACG GCCCAGGACT GCGAGCGGGG CTTCCTCACC
    CGCGAGCTCT CGCTCGTCAC CCACGCCCTG CACCACTTCT GGCTGCACAA CCTCTGTGAT
    GTCTACTTGG AGGCTGTGAA GCCGGTGCTA TCACACTCTC CCCGCCCTCT GGAGCCGCCT
    CAGGTCCTGT TCTTCTGCGC AGACGTCGGT CTCCGCCTCC TCGCCCCGTT GATGCCTTTC
    CTGGCTGAAG AGCTCTGGCA GAGGCTGCCC CCCAGGCCTG GTGGTCCCTC TGCCCCCAGC
    ATCTGTGTTG CCCCCTACCC AAGTGCCCGC AGCTTGTGCT GCTGCAGAGC TCAGAGCCAG
    GCGAGCAGGG CCTCTTTGAG GCCTTCCTGG AGCCGCTGGG CATCCTGGGC TACTGTGGGG
    CGGTGGGCCT TCTACCCCCT GGTGCAGCAG CCCCCTCAGG CTGGGTCCAG GCCGCCCTCA
    GTGACACCAG TCAGGCGTAT ATGGAGCTGC AGGGCCTGGT GGACCCCCAG ACCCACCTAC
    CTCGGCTGGC TGCCCGAAGA CACAAGTTGC AGAAGCAGCT TGATGGCCTC CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa39654
    Clone ID Related Accession (Same CDS sequence) NM_001256544.1 , XM_005223581.4 , XM_005223581.3
    Accession Version NM_001256544.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3198bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-28
    Organism Bos taurus(cattle)
    Product valine--tRNA ligase, mitochondrial
    Comment Comment: INFERRED REFSEQ: This record is predicted by genome sequence analysis and is not yet supported by experimental evidence. The reference sequence was derived from NKLS02000023.1. On Feb 8, 2012 this sequence version replaced XM_003587700.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR6764281.34599.1, SRR5571262.27742.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGCCTCATT TACCTCTGAC CTCTTTTCGA CCACCGCTTT GGGGCTTGAG GCCCTCACGG 
    GGCCTCCCCA GGTCCCGTCC CCTTTCCACC CAGTCAGAGC CAAATGGATC AGCCATCTCC
    CGGAGGAACC GTGAAGCCAA ACAGAAGCGC CTGCGAGAGA AGCAGGCATC GCTGGAGGCT
    GGGATAGCCC CCAAGAGCAA GTCACCTGCA GAATCCAGTA AGGCCTGGAC CCCTAAGGAG
    AGAGTGTTGT ATGAAATCCC CACGGAACAT GGAGAAAAGA AAGATGTCTC CCGACCCCTG
    CCTCCTGCAT ACAGCCCCCA GTACGTTGAG GCTGCCTGGT ACCCTTGGTG GGTGCGAGAG
    GGCTTCTTCA AACCAGAATA TCAGGCCAGG CTGCCCCAGG CCACAGGGGA GACCTTTTCC
    ATGTGTATCC CACCCCCTAA TGTCACAGGC TCCCTGCACA TTGGGCATGC GCTGACCGTG
    GCCATACAGG ATGCTCTCGT GCGCTGGCAC CGGATGCGTG GGGATCGGGT GCTGTGGGTC
    CCTGGCTCAG ATCATGCAGG GATTGCAACC CAAGCTGTGG TGGAGAAACA GCTGTGGAAG
    GAGCGAGGCG TGAGGAGACA TGAGCTGAGC CGGGAAGACT TCCTTAGGGA GGTGTGGAAG
    TGGAAGGAGG AGAAAGGTGG CGAGATCTGT GAGCAGCTCC GAGCGCTGGG GGCCTCCCTG
    GACTGGGACC GAGAGTGCTT TACCATGGAT ACCGGCTCCT CAGTGGCCGT GACAGAAGCT
    TTCGTGCGGC TCTACAAGGC TGGGTTGTTG TACCGGAGCC GGCAGCTGGT CAATTGGTCG
    TGTGCCTTAC GCTCCGCCAT CTCGGATATT GAGGTGGAGA GCCGGCCCCT CCCTGGCCGC
    ACGGAGCTTC AGTTGCCTGG CTGCCCCACC CCCGTGTCTT TCGGCCTCCT CTTTTCTGTG
    GCCTTCCCCG TGGATGGAGA GCCTGATGCA GAGGTTGTGG TGGGAACCAC GAGGCCAGAG
    ACGCTGCCTG GAGACGTGGC TGTGGCGGTC CATCCTGATG ATTCCCGCTA CACGCATCTA
    CATGGGCGAC AACTCTGTCA TCCCTTGACG GGGCAGCTTC TCCCTCTTAT CACAGACTGC
    ACTGTTCAGC CACACCTGGG CACAGGGGCG GTGAAGGTGA CTCCAGCGCA CAGCCCTGCC
    GATGCTGAGC TGGGGGCCCG ACACGGCCTG AGCCCCCGGA GTGTCATTGC GGAGGATGGG
    ACCATGACCG CCCTTTGTGA GGACTGGCTG CAGGGTCTTC ACCGATTTGT GGCCCGGGAA
    AAGATTCTGT CAGCACTGAG GGAGCGGGGC CTGTTCCGGG GCCTCCAGAA CCACCCCATG
    GTGCTGCCCA TTTGCAGCCG TTCCGGGGAC GTGGTAGAGT ACCTACTGAA GAGCCAGTGG
    TTTGTCCGCT GCCGGGAAAT GGGGGAGCGA GCCGCCAAGG CCGTGGAGTC GGGGGCCCTG
    GACCTCAGTC CCTCCTTCCA CCAGAAGAAC TGGCAGCACT GGTTTTCCCA CATTGGGGAC
    TGGTGTGTCT CCCGGCAGCT GTGGTGGGGC CATCGGATTC CAGCCTACCT GGTTGTAGAG
    GAACATGCAA AGGGTGAAAC GGAGGACTTC TGGGTGGTCG GGCGGACAGA GGCTGAGGCC
    AGAGAAGTAG CTGCAGAATT AACAGGGAGA CCAGGGGCGG AGCTGACCCT GGAGAGGGAC
    CCTGATGTCC TGGACACGTG GTTCTCCTCC GCTCTTTTCC CCTTTTCTGC CCTGGGCTGG
    CCCCGAGAGA CCCCGGACCT GGCTCGTTTC TACCCCCTGT CACTTTTGGA AACGGGCAGT
    GACCTCCTGC TCTTCTGGGT GGGCCGCATG GTCATGTTGG GGACCCAGCT CACAGGGCAG
    CTCCCCTTCA GCAAGGTCCT GCTTCACTCC ATGGTCCGGG ACAGGCAGGG CCGGAAGATG
    AGCAAGTCCC TGGGGAACGT GCTGGACCCA CGGGACATCA TCAGTGGGGT GGAGCTGCAG
    GTACTGCAGG ACAATCTGAG GGATGGAAAC TTGGACCCCG CAGAGCTGGC GATTGCAGCC
    TCAGCGCAGA GAAAGGACTT CCCTCATGGG ATCCCCGAGT GTGGGACAGA TGCTCTGAGG
    TTCACCCTGT GCTCCCATGG GGCCCTGGGA GGTGACTTGC ACCTGTCCGT CTCTGAGGTC
    CTGAGCTTCC GACACTTCTG CAATAAGATC TGGAATGCCC TGCGCTTTAT CCTGAATGCT
    CTTGGGGAGG AATTCATACC CCAACCTGCG GAGGAGCTGT CCCCCGCCTC CCGCATGGAC
    ACCTGGATCC TCAGCTGCCT GGCCCGCACG GCCCAGGACT GCGAGCGGGG CTTCCTCACC
    CGCGAGCTCT CGCTCGTCAC CCACGCCCTG CACCACTTCT GGCTGCACAA CCTCTGTGAT
    GTCTACTTGG AGGCTGTGAA GCCGGTGCTA TCACACTCTC CCCGCCCTCT GGAGCCGCCT
    CAGGTCCTGT TCTTCTGCGC AGACGTCGGT CTCCGCCTCC TCGCCCCGTT GATGCCTTTC
    CTGGCTGAAG AGCTCTGGCA GAGGCTGCCC CCCAGGCCTG GTGGTCCCTC TGCCCCCAGC
    ATCTGTGTTG CCCCCTACCC AAGTGCCCGC AGCTTGGAGC ACTGGCACCA GCCTGAGCTG
    GAGAGGCGCT TCTCCCGGGT TCAGGAGTCC GTGCAGGTGC TCAGGGCTCT GCGCGCCACC
    TACCAGCTCA CCAAGGCCCG GCCCCGAGTG CTGCTGCAGA GCTCAGAGCC AGGCGAGCAG
    GGCCTCTTTG AGGCCTTCCT GGAGCCGCTG GGCATCCTGG GCTACTGTGG GGCGGTGGGC
    CTTCTACCCC CTGGTGCAGC AGCCCCCTCA GGCTGGGTCC AGGCCGCCCT CAGTGACACC
    AGTCAGGCGT ATATGGAGCT GCAGGGCCTG GTGGACCCCC AGACCCACCT ACCTCGGCTG
    GCTGCCCGAA GACACAAGTT GCAGAAGCAG CTTGATGGCC TCCTAGCCCG GCCCCCATCC
    GAGGGACAGG CGGAGACTCA GAGGCAGCAG AGGCTTTCTT CCCTCCAGTT GGAGTTGTCA
    AAACTGGACA AGGCGGCCTC TCACCTCCAG CAGCTGATGG ATGATTTTCC AAGCCCTGGG
    AGCTCACCAT CCCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001243473.1
    CDS109..3306
    Translation

    Target ORF information:

    RefSeq Version NM_001256544.1
    Organism Bos taurus(cattle)
    Definition Bos taurus valyl-tRNA synthetase 2, mitochondrial (VARS2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001256544.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGCCTCATT TACCTCTGAC CTCTTTTCGA CCACCGCTTT GGGGCTTGAG GCCCTCACGG 
    GGCCTCCCCA GGTCCCGTCC CCTTTCCACC CAGTCAGAGC CAAATGGATC AGCCATCTCC
    CGGAGGAACC GTGAAGCCAA ACAGAAGCGC CTGCGAGAGA AGCAGGCATC GCTGGAGGCT
    GGGATAGCCC CCAAGAGCAA GTCACCTGCA GAATCCAGTA AGGCCTGGAC CCCTAAGGAG
    AGAGTGTTGT ATGAAATCCC CACGGAACAT GGAGAAAAGA AAGATGTCTC CCGACCCCTG
    CCTCCTGCAT ACAGCCCCCA GTACGTTGAG GCTGCCTGGT ACCCTTGGTG GGTGCGAGAG
    GGCTTCTTCA AACCAGAATA TCAGGCCAGG CTGCCCCAGG CCACAGGGGA GACCTTTTCC
    ATGTGTATCC CACCCCCTAA TGTCACAGGC TCCCTGCACA TTGGGCATGC GCTGACCGTG
    GCCATACAGG ATGCTCTCGT GCGCTGGCAC CGGATGCGTG GGGATCGGGT GCTGTGGGTC
    CCTGGCTCAG ATCATGCAGG GATTGCAACC CAAGCTGTGG TGGAGAAACA GCTGTGGAAG
    GAGCGAGGCG TGAGGAGACA TGAGCTGAGC CGGGAAGACT TCCTTAGGGA GGTGTGGAAG
    TGGAAGGAGG AGAAAGGTGG CGAGATCTGT GAGCAGCTCC GAGCGCTGGG GGCCTCCCTG
    GACTGGGACC GAGAGTGCTT TACCATGGAT ACCGGCTCCT CAGTGGCCGT GACAGAAGCT
    TTCGTGCGGC TCTACAAGGC TGGGTTGTTG TACCGGAGCC GGCAGCTGGT CAATTGGTCG
    TGTGCCTTAC GCTCCGCCAT CTCGGATATT GAGGTGGAGA GCCGGCCCCT CCCTGGCCGC
    ACGGAGCTTC AGTTGCCTGG CTGCCCCACC CCCGTGTCTT TCGGCCTCCT CTTTTCTGTG
    GCCTTCCCCG TGGATGGAGA GCCTGATGCA GAGGTTGTGG TGGGAACCAC GAGGCCAGAG
    ACGCTGCCTG GAGACGTGGC TGTGGCGGTC CATCCTGATG ATTCCCGCTA CACGCATCTA
    CATGGGCGAC AACTCTGTCA TCCCTTGACG GGGCAGCTTC TCCCTCTTAT CACAGACTGC
    ACTGTTCAGC CACACCTGGG CACAGGGGCG GTGAAGGTGA CTCCAGCGCA CAGCCCTGCC
    GATGCTGAGC TGGGGGCCCG ACACGGCCTG AGCCCCCGGA GTGTCATTGC GGAGGATGGG
    ACCATGACCG CCCTTTGTGA GGACTGGCTG CAGGGTCTTC ACCGATTTGT GGCCCGGGAA
    AAGATTCTGT CAGCACTGAG GGAGCGGGGC CTGTTCCGGG GCCTCCAGAA CCACCCCATG
    GTGCTGCCCA TTTGCAGCCG TTCCGGGGAC GTGGTAGAGT ACCTACTGAA GAGCCAGTGG
    TTTGTCCGCT GCCGGGAAAT GGGGGAGCGA GCCGCCAAGG CCGTGGAGTC GGGGGCCCTG
    GACCTCAGTC CCTCCTTCCA CCAGAAGAAC TGGCAGCACT GGTTTTCCCA CATTGGGGAC
    TGGTGTGTCT CCCGGCAGCT GTGGTGGGGC CATCGGATTC CAGCCTACCT GGTTGTAGAG
    GAACATGCAA AGGGTGAAAC GGAGGACTTC TGGGTGGTCG GGCGGACAGA GGCTGAGGCC
    AGAGAAGTAG CTGCAGAATT AACAGGGAGA CCAGGGGCGG AGCTGACCCT GGAGAGGGAC
    CCTGATGTCC TGGACACGTG GTTCTCCTCC GCTCTTTTCC CCTTTTCTGC CCTGGGCTGG
    CCCCGAGAGA CCCCGGACCT GGCTCGTTTC TACCCCCTGT CACTTTTGGA AACGGGCAGT
    GACCTCCTGC TCTTCTGGGT GGGCCGCATG GTCATGTTGG GGACCCAGCT CACAGGGCAG
    CTCCCCTTCA GCAAGGTCCT GCTTCACTCC ATGGTCCGGG ACAGGCAGGG CCGGAAGATG
    AGCAAGTCCC TGGGGAACGT GCTGGACCCA CGGGACATCA TCAGTGGGGT GGAGCTGCAG
    GTACTGCAGG ACAATCTGAG GGATGGAAAC TTGGACCCCG CAGAGCTGGC GATTGCAGCC
    TCAGCGCAGA GAAAGGACTT CCCTCATGGG ATCCCCGAGT GTGGGACAGA TGCTCTGAGG
    TTCACCCTGT GCTCCCATGG GGCCCTGGGA GGTGACTTGC ACCTGTCCGT CTCTGAGGTC
    CTGAGCTTCC GACACTTCTG CAATAAGATC TGGAATGCCC TGCGCTTTAT CCTGAATGCT
    CTTGGGGAGG AATTCATACC CCAACCTGCG GAGGAGCTGT CCCCCGCCTC CCGCATGGAC
    ACCTGGATCC TCAGCTGCCT GGCCCGCACG GCCCAGGACT GCGAGCGGGG CTTCCTCACC
    CGCGAGCTCT CGCTCGTCAC CCACGCCCTG CACCACTTCT GGCTGCACAA CCTCTGTGAT
    GTCTACTTGG AGGCTGTGAA GCCGGTGCTA TCACACTCTC CCCGCCCTCT GGAGCCGCCT
    CAGGTCCTGT TCTTCTGCGC AGACGTCGGT CTCCGCCTCC TCGCCCCGTT GATGCCTTTC
    CTGGCTGAAG AGCTCTGGCA GAGGCTGCCC CCCAGGCCTG GTGGTCCCTC TGCCCCCAGC
    ATCTGTGTTG CCCCCTACCC AAGTGCCCGC AGCTTGGAGC ACTGGCACCA GCCTGAGCTG
    GAGAGGCGCT TCTCCCGGGT TCAGGAGTCC GTGCAGGTGC TCAGGGCTCT GCGCGCCACC
    TACCAGCTCA CCAAGGCCCG GCCCCGAGTG CTGCTGCAGA GCTCAGAGCC AGGCGAGCAG
    GGCCTCTTTG AGGCCTTCCT GGAGCCGCTG GGCATCCTGG GCTACTGTGG GGCGGTGGGC
    CTTCTACCCC CTGGTGCAGC AGCCCCCTCA GGCTGGGTCC AGGCCGCCCT CAGTGACACC
    AGTCAGGCGT ATATGGAGCT GCAGGGCCTG GTGGACCCCC AGACCCACCT ACCTCGGCTG
    GCTGCCCGAA GACACAAGTT GCAGAAGCAG CTTGATGGCC TCCTAGCCCG GCCCCCATCC
    GAGGGACAGG CGGAGACTCA GAGGCAGCAG AGGCTTTCTT CCCTCCAGTT GGAGTTGTCA
    AAACTGGACA AGGCGGCCTC TCACCTCCAG CAGCTGATGG ATGATTTTCC AAGCCCTGGG
    AGCTCACCAT CCCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa39654
    Clone ID Related Accession (Same CDS sequence) NM_001256544.1 , XM_005223581.4 , XM_005223581.3
    Accession Version XM_005223581.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3198bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product valine--tRNA ligase, mitochondrial isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005223581.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGCCTCATT TACCTCTGAC CTCTTTTCGA CCACCGCTTT GGGGCTTGAG GCCCTCACGG 
    GGCCTCCCCA GGTCCCGTCC CCTTTCCACC CAGTCAGAGC CAAATGGATC AGCCATCTCC
    CGGAGGAACC GTGAAGCCAA ACAGAAGCGC CTGCGAGAGA AGCAGGCATC GCTGGAGGCT
    GGGATAGCCC CCAAGAGCAA GTCACCTGCA GAATCCAGTA AGGCCTGGAC CCCTAAGGAG
    AGAGTGTTGT ATGAAATCCC CACGGAACAT GGAGAAAAGA AAGATGTCTC CCGACCCCTG
    CCTCCTGCAT ACAGCCCCCA GTACGTTGAG GCTGCCTGGT ACCCTTGGTG GGTGCGAGAG
    GGCTTCTTCA AACCAGAATA TCAGGCCAGG CTGCCCCAGG CCACAGGGGA GACCTTTTCC
    ATGTGTATCC CACCCCCTAA TGTCACAGGC TCCCTGCACA TTGGGCATGC GCTGACCGTG
    GCCATACAGG ATGCTCTCGT GCGCTGGCAC CGGATGCGTG GGGATCGGGT GCTGTGGGTC
    CCTGGCTCAG ATCATGCAGG GATTGCAACC CAAGCTGTGG TGGAGAAACA GCTGTGGAAG
    GAGCGAGGCG TGAGGAGACA TGAGCTGAGC CGGGAAGACT TCCTTAGGGA GGTGTGGAAG
    TGGAAGGAGG AGAAAGGTGG CGAGATCTGT GAGCAGCTCC GAGCGCTGGG GGCCTCCCTG
    GACTGGGACC GAGAGTGCTT TACCATGGAT ACCGGCTCCT CAGTGGCCGT GACAGAAGCT
    TTCGTGCGGC TCTACAAGGC TGGGTTGTTG TACCGGAGCC GGCAGCTGGT CAATTGGTCG
    TGTGCCTTAC GCTCCGCCAT CTCGGATATT GAGGTGGAGA GCCGGCCCCT CCCTGGCCGC
    ACGGAGCTTC AGTTGCCTGG CTGCCCCACC CCCGTGTCTT TCGGCCTCCT CTTTTCTGTG
    GCCTTCCCCG TGGATGGAGA GCCTGATGCA GAGGTTGTGG TGGGAACCAC GAGGCCAGAG
    ACGCTGCCTG GAGACGTGGC TGTGGCGGTC CATCCTGATG ATTCCCGCTA CACGCATCTA
    CATGGGCGAC AACTCTGTCA TCCCTTGACG GGGCAGCTTC TCCCTCTTAT CACAGACTGC
    ACTGTTCAGC CACACCTGGG CACAGGGGCG GTGAAGGTGA CTCCAGCGCA CAGCCCTGCC
    GATGCTGAGC TGGGGGCCCG ACACGGCCTG AGCCCCCGGA GTGTCATTGC GGAGGATGGG
    ACCATGACCG CCCTTTGTGA GGACTGGCTG CAGGGTCTTC ACCGATTTGT GGCCCGGGAA
    AAGATTCTGT CAGCACTGAG GGAGCGGGGC CTGTTCCGGG GCCTCCAGAA CCACCCCATG
    GTGCTGCCCA TTTGCAGCCG TTCCGGGGAC GTGGTAGAGT ACCTACTGAA GAGCCAGTGG
    TTTGTCCGCT GCCGGGAAAT GGGGGAGCGA GCCGCCAAGG CCGTGGAGTC GGGGGCCCTG
    GACCTCAGTC CCTCCTTCCA CCAGAAGAAC TGGCAGCACT GGTTTTCCCA CATTGGGGAC
    TGGTGTGTCT CCCGGCAGCT GTGGTGGGGC CATCGGATTC CAGCCTACCT GGTTGTAGAG
    GAACATGCAA AGGGTGAAAC GGAGGACTTC TGGGTGGTCG GGCGGACAGA GGCTGAGGCC
    AGAGAAGTAG CTGCAGAATT AACAGGGAGA CCAGGGGCGG AGCTGACCCT GGAGAGGGAC
    CCTGATGTCC TGGACACGTG GTTCTCCTCC GCTCTTTTCC CCTTTTCTGC CCTGGGCTGG
    CCCCGAGAGA CCCCGGACCT GGCTCGTTTC TACCCCCTGT CACTTTTGGA AACGGGCAGT
    GACCTCCTGC TCTTCTGGGT GGGCCGCATG GTCATGTTGG GGACCCAGCT CACAGGGCAG
    CTCCCCTTCA GCAAGGTCCT GCTTCACTCC ATGGTCCGGG ACAGGCAGGG CCGGAAGATG
    AGCAAGTCCC TGGGGAACGT GCTGGACCCA CGGGACATCA TCAGTGGGGT GGAGCTGCAG
    GTACTGCAGG ACAATCTGAG GGATGGAAAC TTGGACCCCG CAGAGCTGGC GATTGCAGCC
    TCAGCGCAGA GAAAGGACTT CCCTCATGGG ATCCCCGAGT GTGGGACAGA TGCTCTGAGG
    TTCACCCTGT GCTCCCATGG GGCCCTGGGA GGTGACTTGC ACCTGTCCGT CTCTGAGGTC
    CTGAGCTTCC GACACTTCTG CAATAAGATC TGGAATGCCC TGCGCTTTAT CCTGAATGCT
    CTTGGGGAGG AATTCATACC CCAACCTGCG GAGGAGCTGT CCCCCGCCTC CCGCATGGAC
    ACCTGGATCC TCAGCTGCCT GGCCCGCACG GCCCAGGACT GCGAGCGGGG CTTCCTCACC
    CGCGAGCTCT CGCTCGTCAC CCACGCCCTG CACCACTTCT GGCTGCACAA CCTCTGTGAT
    GTCTACTTGG AGGCTGTGAA GCCGGTGCTA TCACACTCTC CCCGCCCTCT GGAGCCGCCT
    CAGGTCCTGT TCTTCTGCGC AGACGTCGGT CTCCGCCTCC TCGCCCCGTT GATGCCTTTC
    CTGGCTGAAG AGCTCTGGCA GAGGCTGCCC CCCAGGCCTG GTGGTCCCTC TGCCCCCAGC
    ATCTGTGTTG CCCCCTACCC AAGTGCCCGC AGCTTGGAGC ACTGGCACCA GCCTGAGCTG
    GAGAGGCGCT TCTCCCGGGT TCAGGAGTCC GTGCAGGTGC TCAGGGCTCT GCGCGCCACC
    TACCAGCTCA CCAAGGCCCG GCCCCGAGTG CTGCTGCAGA GCTCAGAGCC AGGCGAGCAG
    GGCCTCTTTG AGGCCTTCCT GGAGCCGCTG GGCATCCTGG GCTACTGTGG GGCGGTGGGC
    CTTCTACCCC CTGGTGCAGC AGCCCCCTCA GGCTGGGTCC AGGCCGCCCT CAGTGACACC
    AGTCAGGCGT ATATGGAGCT GCAGGGCCTG GTGGACCCCC AGACCCACCT ACCTCGGCTG
    GCTGCCCGAA GACACAAGTT GCAGAAGCAG CTTGATGGCC TCCTAGCCCG GCCCCCATCC
    GAGGGACAGG CGGAGACTCA GAGGCAGCAG AGGCTTTCTT CCCTCCAGTT GGAGTTGTCA
    AAACTGGACA AGGCGGCCTC TCACCTCCAG CAGCTGATGG ATGATTTTCC AAGCCCTGGG
    AGCTCACCAT CCCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005223638.2
    CDS223..3420
    Translation

    Target ORF information:

    RefSeq Version XM_005223581.4
    Organism Bos taurus(cattle)
    Definition Bos taurus valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005223581.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGCCTCATT TACCTCTGAC CTCTTTTCGA CCACCGCTTT GGGGCTTGAG GCCCTCACGG 
    GGCCTCCCCA GGTCCCGTCC CCTTTCCACC CAGTCAGAGC CAAATGGATC AGCCATCTCC
    CGGAGGAACC GTGAAGCCAA ACAGAAGCGC CTGCGAGAGA AGCAGGCATC GCTGGAGGCT
    GGGATAGCCC CCAAGAGCAA GTCACCTGCA GAATCCAGTA AGGCCTGGAC CCCTAAGGAG
    AGAGTGTTGT ATGAAATCCC CACGGAACAT GGAGAAAAGA AAGATGTCTC CCGACCCCTG
    CCTCCTGCAT ACAGCCCCCA GTACGTTGAG GCTGCCTGGT ACCCTTGGTG GGTGCGAGAG
    GGCTTCTTCA AACCAGAATA TCAGGCCAGG CTGCCCCAGG CCACAGGGGA GACCTTTTCC
    ATGTGTATCC CACCCCCTAA TGTCACAGGC TCCCTGCACA TTGGGCATGC GCTGACCGTG
    GCCATACAGG ATGCTCTCGT GCGCTGGCAC CGGATGCGTG GGGATCGGGT GCTGTGGGTC
    CCTGGCTCAG ATCATGCAGG GATTGCAACC CAAGCTGTGG TGGAGAAACA GCTGTGGAAG
    GAGCGAGGCG TGAGGAGACA TGAGCTGAGC CGGGAAGACT TCCTTAGGGA GGTGTGGAAG
    TGGAAGGAGG AGAAAGGTGG CGAGATCTGT GAGCAGCTCC GAGCGCTGGG GGCCTCCCTG
    GACTGGGACC GAGAGTGCTT TACCATGGAT ACCGGCTCCT CAGTGGCCGT GACAGAAGCT
    TTCGTGCGGC TCTACAAGGC TGGGTTGTTG TACCGGAGCC GGCAGCTGGT CAATTGGTCG
    TGTGCCTTAC GCTCCGCCAT CTCGGATATT GAGGTGGAGA GCCGGCCCCT CCCTGGCCGC
    ACGGAGCTTC AGTTGCCTGG CTGCCCCACC CCCGTGTCTT TCGGCCTCCT CTTTTCTGTG
    GCCTTCCCCG TGGATGGAGA GCCTGATGCA GAGGTTGTGG TGGGAACCAC GAGGCCAGAG
    ACGCTGCCTG GAGACGTGGC TGTGGCGGTC CATCCTGATG ATTCCCGCTA CACGCATCTA
    CATGGGCGAC AACTCTGTCA TCCCTTGACG GGGCAGCTTC TCCCTCTTAT CACAGACTGC
    ACTGTTCAGC CACACCTGGG CACAGGGGCG GTGAAGGTGA CTCCAGCGCA CAGCCCTGCC
    GATGCTGAGC TGGGGGCCCG ACACGGCCTG AGCCCCCGGA GTGTCATTGC GGAGGATGGG
    ACCATGACCG CCCTTTGTGA GGACTGGCTG CAGGGTCTTC ACCGATTTGT GGCCCGGGAA
    AAGATTCTGT CAGCACTGAG GGAGCGGGGC CTGTTCCGGG GCCTCCAGAA CCACCCCATG
    GTGCTGCCCA TTTGCAGCCG TTCCGGGGAC GTGGTAGAGT ACCTACTGAA GAGCCAGTGG
    TTTGTCCGCT GCCGGGAAAT GGGGGAGCGA GCCGCCAAGG CCGTGGAGTC GGGGGCCCTG
    GACCTCAGTC CCTCCTTCCA CCAGAAGAAC TGGCAGCACT GGTTTTCCCA CATTGGGGAC
    TGGTGTGTCT CCCGGCAGCT GTGGTGGGGC CATCGGATTC CAGCCTACCT GGTTGTAGAG
    GAACATGCAA AGGGTGAAAC GGAGGACTTC TGGGTGGTCG GGCGGACAGA GGCTGAGGCC
    AGAGAAGTAG CTGCAGAATT AACAGGGAGA CCAGGGGCGG AGCTGACCCT GGAGAGGGAC
    CCTGATGTCC TGGACACGTG GTTCTCCTCC GCTCTTTTCC CCTTTTCTGC CCTGGGCTGG
    CCCCGAGAGA CCCCGGACCT GGCTCGTTTC TACCCCCTGT CACTTTTGGA AACGGGCAGT
    GACCTCCTGC TCTTCTGGGT GGGCCGCATG GTCATGTTGG GGACCCAGCT CACAGGGCAG
    CTCCCCTTCA GCAAGGTCCT GCTTCACTCC ATGGTCCGGG ACAGGCAGGG CCGGAAGATG
    AGCAAGTCCC TGGGGAACGT GCTGGACCCA CGGGACATCA TCAGTGGGGT GGAGCTGCAG
    GTACTGCAGG ACAATCTGAG GGATGGAAAC TTGGACCCCG CAGAGCTGGC GATTGCAGCC
    TCAGCGCAGA GAAAGGACTT CCCTCATGGG ATCCCCGAGT GTGGGACAGA TGCTCTGAGG
    TTCACCCTGT GCTCCCATGG GGCCCTGGGA GGTGACTTGC ACCTGTCCGT CTCTGAGGTC
    CTGAGCTTCC GACACTTCTG CAATAAGATC TGGAATGCCC TGCGCTTTAT CCTGAATGCT
    CTTGGGGAGG AATTCATACC CCAACCTGCG GAGGAGCTGT CCCCCGCCTC CCGCATGGAC
    ACCTGGATCC TCAGCTGCCT GGCCCGCACG GCCCAGGACT GCGAGCGGGG CTTCCTCACC
    CGCGAGCTCT CGCTCGTCAC CCACGCCCTG CACCACTTCT GGCTGCACAA CCTCTGTGAT
    GTCTACTTGG AGGCTGTGAA GCCGGTGCTA TCACACTCTC CCCGCCCTCT GGAGCCGCCT
    CAGGTCCTGT TCTTCTGCGC AGACGTCGGT CTCCGCCTCC TCGCCCCGTT GATGCCTTTC
    CTGGCTGAAG AGCTCTGGCA GAGGCTGCCC CCCAGGCCTG GTGGTCCCTC TGCCCCCAGC
    ATCTGTGTTG CCCCCTACCC AAGTGCCCGC AGCTTGGAGC ACTGGCACCA GCCTGAGCTG
    GAGAGGCGCT TCTCCCGGGT TCAGGAGTCC GTGCAGGTGC TCAGGGCTCT GCGCGCCACC
    TACCAGCTCA CCAAGGCCCG GCCCCGAGTG CTGCTGCAGA GCTCAGAGCC AGGCGAGCAG
    GGCCTCTTTG AGGCCTTCCT GGAGCCGCTG GGCATCCTGG GCTACTGTGG GGCGGTGGGC
    CTTCTACCCC CTGGTGCAGC AGCCCCCTCA GGCTGGGTCC AGGCCGCCCT CAGTGACACC
    AGTCAGGCGT ATATGGAGCT GCAGGGCCTG GTGGACCCCC AGACCCACCT ACCTCGGCTG
    GCTGCCCGAA GACACAAGTT GCAGAAGCAG CTTGATGGCC TCCTAGCCCG GCCCCCATCC
    GAGGGACAGG CGGAGACTCA GAGGCAGCAG AGGCTTTCTT CCCTCCAGTT GGAGTTGTCA
    AAACTGGACA AGGCGGCCTC TCACCTCCAG CAGCTGATGG ATGATTTTCC AAGCCCTGGG
    AGCTCACCAT CCCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa39654
    Clone ID Related Accession (Same CDS sequence) NM_001256544.1 , XM_005223581.4 , XM_005223581.3
    Accession Version XM_005223581.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3198bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product valine--tRNA ligase, mitochondrial isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104557.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005223581.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGCCTCATT TACCTCTGAC CTCTTTTCGA CCACCGCTTT GGGGCTTGAG GCCCTCACGG 
    GGCCTCCCCA GGTCCCGTCC CCTTTCCACC CAGTCAGAGC CAAATGGATC AGCCATCTCC
    CGGAGGAACC GTGAAGCCAA ACAGAAGCGC CTGCGAGAGA AGCAGGCATC GCTGGAGGCT
    GGGATAGCCC CCAAGAGCAA GTCACCTGCA GAATCCAGTA AGGCCTGGAC CCCTAAGGAG
    AGAGTGTTGT ATGAAATCCC CACGGAACAT GGAGAAAAGA AAGATGTCTC CCGACCCCTG
    CCTCCTGCAT ACAGCCCCCA GTACGTTGAG GCTGCCTGGT ACCCTTGGTG GGTGCGAGAG
    GGCTTCTTCA AACCAGAATA TCAGGCCAGG CTGCCCCAGG CCACAGGGGA GACCTTTTCC
    ATGTGTATCC CACCCCCTAA TGTCACAGGC TCCCTGCACA TTGGGCATGC GCTGACCGTG
    GCCATACAGG ATGCTCTCGT GCGCTGGCAC CGGATGCGTG GGGATCGGGT GCTGTGGGTC
    CCTGGCTCAG ATCATGCAGG GATTGCAACC CAAGCTGTGG TGGAGAAACA GCTGTGGAAG
    GAGCGAGGCG TGAGGAGACA TGAGCTGAGC CGGGAAGACT TCCTTAGGGA GGTGTGGAAG
    TGGAAGGAGG AGAAAGGTGG CGAGATCTGT GAGCAGCTCC GAGCGCTGGG GGCCTCCCTG
    GACTGGGACC GAGAGTGCTT TACCATGGAT ACCGGCTCCT CAGTGGCCGT GACAGAAGCT
    TTCGTGCGGC TCTACAAGGC TGGGTTGTTG TACCGGAGCC GGCAGCTGGT CAATTGGTCG
    TGTGCCTTAC GCTCCGCCAT CTCGGATATT GAGGTGGAGA GCCGGCCCCT CCCTGGCCGC
    ACGGAGCTTC AGTTGCCTGG CTGCCCCACC CCCGTGTCTT TCGGCCTCCT CTTTTCTGTG
    GCCTTCCCCG TGGATGGAGA GCCTGATGCA GAGGTTGTGG TGGGAACCAC GAGGCCAGAG
    ACGCTGCCTG GAGACGTGGC TGTGGCGGTC CATCCTGATG ATTCCCGCTA CACGCATCTA
    CATGGGCGAC AACTCTGTCA TCCCTTGACG GGGCAGCTTC TCCCTCTTAT CACAGACTGC
    ACTGTTCAGC CACACCTGGG CACAGGGGCG GTGAAGGTGA CTCCAGCGCA CAGCCCTGCC
    GATGCTGAGC TGGGGGCCCG ACACGGCCTG AGCCCCCGGA GTGTCATTGC GGAGGATGGG
    ACCATGACCG CCCTTTGTGA GGACTGGCTG CAGGGTCTTC ACCGATTTGT GGCCCGGGAA
    AAGATTCTGT CAGCACTGAG GGAGCGGGGC CTGTTCCGGG GCCTCCAGAA CCACCCCATG
    GTGCTGCCCA TTTGCAGCCG TTCCGGGGAC GTGGTAGAGT ACCTACTGAA GAGCCAGTGG
    TTTGTCCGCT GCCGGGAAAT GGGGGAGCGA GCCGCCAAGG CCGTGGAGTC GGGGGCCCTG
    GACCTCAGTC CCTCCTTCCA CCAGAAGAAC TGGCAGCACT GGTTTTCCCA CATTGGGGAC
    TGGTGTGTCT CCCGGCAGCT GTGGTGGGGC CATCGGATTC CAGCCTACCT GGTTGTAGAG
    GAACATGCAA AGGGTGAAAC GGAGGACTTC TGGGTGGTCG GGCGGACAGA GGCTGAGGCC
    AGAGAAGTAG CTGCAGAATT AACAGGGAGA CCAGGGGCGG AGCTGACCCT GGAGAGGGAC
    CCTGATGTCC TGGACACGTG GTTCTCCTCC GCTCTTTTCC CCTTTTCTGC CCTGGGCTGG
    CCCCGAGAGA CCCCGGACCT GGCTCGTTTC TACCCCCTGT CACTTTTGGA AACGGGCAGT
    GACCTCCTGC TCTTCTGGGT GGGCCGCATG GTCATGTTGG GGACCCAGCT CACAGGGCAG
    CTCCCCTTCA GCAAGGTCCT GCTTCACTCC ATGGTCCGGG ACAGGCAGGG CCGGAAGATG
    AGCAAGTCCC TGGGGAACGT GCTGGACCCA CGGGACATCA TCAGTGGGGT GGAGCTGCAG
    GTACTGCAGG ACAATCTGAG GGATGGAAAC TTGGACCCCG CAGAGCTGGC GATTGCAGCC
    TCAGCGCAGA GAAAGGACTT CCCTCATGGG ATCCCCGAGT GTGGGACAGA TGCTCTGAGG
    TTCACCCTGT GCTCCCATGG GGCCCTGGGA GGTGACTTGC ACCTGTCCGT CTCTGAGGTC
    CTGAGCTTCC GACACTTCTG CAATAAGATC TGGAATGCCC TGCGCTTTAT CCTGAATGCT
    CTTGGGGAGG AATTCATACC CCAACCTGCG GAGGAGCTGT CCCCCGCCTC CCGCATGGAC
    ACCTGGATCC TCAGCTGCCT GGCCCGCACG GCCCAGGACT GCGAGCGGGG CTTCCTCACC
    CGCGAGCTCT CGCTCGTCAC CCACGCCCTG CACCACTTCT GGCTGCACAA CCTCTGTGAT
    GTCTACTTGG AGGCTGTGAA GCCGGTGCTA TCACACTCTC CCCGCCCTCT GGAGCCGCCT
    CAGGTCCTGT TCTTCTGCGC AGACGTCGGT CTCCGCCTCC TCGCCCCGTT GATGCCTTTC
    CTGGCTGAAG AGCTCTGGCA GAGGCTGCCC CCCAGGCCTG GTGGTCCCTC TGCCCCCAGC
    ATCTGTGTTG CCCCCTACCC AAGTGCCCGC AGCTTGGAGC ACTGGCACCA GCCTGAGCTG
    GAGAGGCGCT TCTCCCGGGT TCAGGAGTCC GTGCAGGTGC TCAGGGCTCT GCGCGCCACC
    TACCAGCTCA CCAAGGCCCG GCCCCGAGTG CTGCTGCAGA GCTCAGAGCC AGGCGAGCAG
    GGCCTCTTTG AGGCCTTCCT GGAGCCGCTG GGCATCCTGG GCTACTGTGG GGCGGTGGGC
    CTTCTACCCC CTGGTGCAGC AGCCCCCTCA GGCTGGGTCC AGGCCGCCCT CAGTGACACC
    AGTCAGGCGT ATATGGAGCT GCAGGGCCTG GTGGACCCCC AGACCCACCT ACCTCGGCTG
    GCTGCCCGAA GACACAAGTT GCAGAAGCAG CTTGATGGCC TCCTAGCCCG GCCCCCATCC
    GAGGGACAGG CGGAGACTCA GAGGCAGCAG AGGCTTTCTT CCCTCCAGTT GGAGTTGTCA
    AAACTGGACA AGGCGGCCTC TCACCTCCAG CAGCTGATGG ATGATTTTCC AAGCCCTGGG
    AGCTCACCAT CCCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005223638.2
    CDS230..3427
    Translation

    Target ORF information:

    RefSeq Version XM_005223581.3
    Organism Bos taurus(cattle)
    Definition Bos taurus valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005223581.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGCCTCATT TACCTCTGAC CTCTTTTCGA CCACCGCTTT GGGGCTTGAG GCCCTCACGG 
    GGCCTCCCCA GGTCCCGTCC CCTTTCCACC CAGTCAGAGC CAAATGGATC AGCCATCTCC
    CGGAGGAACC GTGAAGCCAA ACAGAAGCGC CTGCGAGAGA AGCAGGCATC GCTGGAGGCT
    GGGATAGCCC CCAAGAGCAA GTCACCTGCA GAATCCAGTA AGGCCTGGAC CCCTAAGGAG
    AGAGTGTTGT ATGAAATCCC CACGGAACAT GGAGAAAAGA AAGATGTCTC CCGACCCCTG
    CCTCCTGCAT ACAGCCCCCA GTACGTTGAG GCTGCCTGGT ACCCTTGGTG GGTGCGAGAG
    GGCTTCTTCA AACCAGAATA TCAGGCCAGG CTGCCCCAGG CCACAGGGGA GACCTTTTCC
    ATGTGTATCC CACCCCCTAA TGTCACAGGC TCCCTGCACA TTGGGCATGC GCTGACCGTG
    GCCATACAGG ATGCTCTCGT GCGCTGGCAC CGGATGCGTG GGGATCGGGT GCTGTGGGTC
    CCTGGCTCAG ATCATGCAGG GATTGCAACC CAAGCTGTGG TGGAGAAACA GCTGTGGAAG
    GAGCGAGGCG TGAGGAGACA TGAGCTGAGC CGGGAAGACT TCCTTAGGGA GGTGTGGAAG
    TGGAAGGAGG AGAAAGGTGG CGAGATCTGT GAGCAGCTCC GAGCGCTGGG GGCCTCCCTG
    GACTGGGACC GAGAGTGCTT TACCATGGAT ACCGGCTCCT CAGTGGCCGT GACAGAAGCT
    TTCGTGCGGC TCTACAAGGC TGGGTTGTTG TACCGGAGCC GGCAGCTGGT CAATTGGTCG
    TGTGCCTTAC GCTCCGCCAT CTCGGATATT GAGGTGGAGA GCCGGCCCCT CCCTGGCCGC
    ACGGAGCTTC AGTTGCCTGG CTGCCCCACC CCCGTGTCTT TCGGCCTCCT CTTTTCTGTG
    GCCTTCCCCG TGGATGGAGA GCCTGATGCA GAGGTTGTGG TGGGAACCAC GAGGCCAGAG
    ACGCTGCCTG GAGACGTGGC TGTGGCGGTC CATCCTGATG ATTCCCGCTA CACGCATCTA
    CATGGGCGAC AACTCTGTCA TCCCTTGACG GGGCAGCTTC TCCCTCTTAT CACAGACTGC
    ACTGTTCAGC CACACCTGGG CACAGGGGCG GTGAAGGTGA CTCCAGCGCA CAGCCCTGCC
    GATGCTGAGC TGGGGGCCCG ACACGGCCTG AGCCCCCGGA GTGTCATTGC GGAGGATGGG
    ACCATGACCG CCCTTTGTGA GGACTGGCTG CAGGGTCTTC ACCGATTTGT GGCCCGGGAA
    AAGATTCTGT CAGCACTGAG GGAGCGGGGC CTGTTCCGGG GCCTCCAGAA CCACCCCATG
    GTGCTGCCCA TTTGCAGCCG TTCCGGGGAC GTGGTAGAGT ACCTACTGAA GAGCCAGTGG
    TTTGTCCGCT GCCGGGAAAT GGGGGAGCGA GCCGCCAAGG CCGTGGAGTC GGGGGCCCTG
    GACCTCAGTC CCTCCTTCCA CCAGAAGAAC TGGCAGCACT GGTTTTCCCA CATTGGGGAC
    TGGTGTGTCT CCCGGCAGCT GTGGTGGGGC CATCGGATTC CAGCCTACCT GGTTGTAGAG
    GAACATGCAA AGGGTGAAAC GGAGGACTTC TGGGTGGTCG GGCGGACAGA GGCTGAGGCC
    AGAGAAGTAG CTGCAGAATT AACAGGGAGA CCAGGGGCGG AGCTGACCCT GGAGAGGGAC
    CCTGATGTCC TGGACACGTG GTTCTCCTCC GCTCTTTTCC CCTTTTCTGC CCTGGGCTGG
    CCCCGAGAGA CCCCGGACCT GGCTCGTTTC TACCCCCTGT CACTTTTGGA AACGGGCAGT
    GACCTCCTGC TCTTCTGGGT GGGCCGCATG GTCATGTTGG GGACCCAGCT CACAGGGCAG
    CTCCCCTTCA GCAAGGTCCT GCTTCACTCC ATGGTCCGGG ACAGGCAGGG CCGGAAGATG
    AGCAAGTCCC TGGGGAACGT GCTGGACCCA CGGGACATCA TCAGTGGGGT GGAGCTGCAG
    GTACTGCAGG ACAATCTGAG GGATGGAAAC TTGGACCCCG CAGAGCTGGC GATTGCAGCC
    TCAGCGCAGA GAAAGGACTT CCCTCATGGG ATCCCCGAGT GTGGGACAGA TGCTCTGAGG
    TTCACCCTGT GCTCCCATGG GGCCCTGGGA GGTGACTTGC ACCTGTCCGT CTCTGAGGTC
    CTGAGCTTCC GACACTTCTG CAATAAGATC TGGAATGCCC TGCGCTTTAT CCTGAATGCT
    CTTGGGGAGG AATTCATACC CCAACCTGCG GAGGAGCTGT CCCCCGCCTC CCGCATGGAC
    ACCTGGATCC TCAGCTGCCT GGCCCGCACG GCCCAGGACT GCGAGCGGGG CTTCCTCACC
    CGCGAGCTCT CGCTCGTCAC CCACGCCCTG CACCACTTCT GGCTGCACAA CCTCTGTGAT
    GTCTACTTGG AGGCTGTGAA GCCGGTGCTA TCACACTCTC CCCGCCCTCT GGAGCCGCCT
    CAGGTCCTGT TCTTCTGCGC AGACGTCGGT CTCCGCCTCC TCGCCCCGTT GATGCCTTTC
    CTGGCTGAAG AGCTCTGGCA GAGGCTGCCC CCCAGGCCTG GTGGTCCCTC TGCCCCCAGC
    ATCTGTGTTG CCCCCTACCC AAGTGCCCGC AGCTTGGAGC ACTGGCACCA GCCTGAGCTG
    GAGAGGCGCT TCTCCCGGGT TCAGGAGTCC GTGCAGGTGC TCAGGGCTCT GCGCGCCACC
    TACCAGCTCA CCAAGGCCCG GCCCCGAGTG CTGCTGCAGA GCTCAGAGCC AGGCGAGCAG
    GGCCTCTTTG AGGCCTTCCT GGAGCCGCTG GGCATCCTGG GCTACTGTGG GGCGGTGGGC
    CTTCTACCCC CTGGTGCAGC AGCCCCCTCA GGCTGGGTCC AGGCCGCCCT CAGTGACACC
    AGTCAGGCGT ATATGGAGCT GCAGGGCCTG GTGGACCCCC AGACCCACCT ACCTCGGCTG
    GCTGCCCGAA GACACAAGTT GCAGAAGCAG CTTGATGGCC TCCTAGCCCG GCCCCCATCC
    GAGGGACAGG CGGAGACTCA GAGGCAGCAG AGGCTTTCTT CCCTCCAGTT GGAGTTGTCA
    AAACTGGACA AGGCGGCCTC TCACCTCCAG CAGCTGATGG ATGATTTTCC AAGCCCTGGG
    AGCTCACCAT CCCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa60389
    Clone ID Related Accession (Same CDS sequence) XM_005223582.3 , XM_005223582.4
    Accession Version XM_005223582.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3078bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product valine--tRNA ligase, mitochondrial isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104557.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005223582.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCCTCATT TACCTCTGAC CTCTTTTCGA CCACCGCTTT GGGGCTTGAG GCCCTCACGG 
    GGCCTCCCCA GGTCCCGTCC CCTTTCCACC CAGTCAGAGC CAAATGGATC AGCCATCTCC
    CGGAGGAACC GTGAAGCCAA ACAGAAGCGC CTGCGAGAGA AGCAGGCATC GCTGGAGGCT
    GGGATAGCCC CCAAGAGCAA GTCACCTGCA GAATCCAGTA AGGCCTGGAC CCCTAAGGAG
    AGAGTGTTGT ATGAAATCCC CACGGAACAT GGAGAAAAGA AAGATGTCTC CCGACCCCTG
    CCTCCTGCAT ACAGCCCCCA GTACGTTGAG GCTGCCTGGT ACCCTTGGTG GGTGCGAGAG
    GGCTTCTTCA AACCAGAATA TCAGGCCAGG CTGCCCCAGG CCACAGGGGA GACCTTTTCC
    ATGTGTATCC CACCCCCTAA TGTCACAGGC TCCCTGCACA TTGGGCATGC GCTGACCGTG
    GCCATACAGG ATGCTCTCGT GCGCTGGCAC CGGATGCGTG GGGATCGGGT GCTGTGGGTC
    CCTGGCTCAG ATCATGCAGG GATTGCAACC CAAGCTGTGG TGGAGAAACA GCTGTGGAAG
    GAGCGAGGCG TGAGGAGACA TGAGCTGAGC CGGGAAGACT TCCTTAGGGA GGTGTGGAAG
    TGGAAGGAGG AGAAAGGTGG CGAGATCTGT GAGCAGCTCC GAGCGCTGGG GGCCTCCCTG
    GACTGGGACC GAGAGTGCTT TACCATGGAT ACCGTGGAGA GCCGGCCCCT CCCTGGCCGC
    ACGGAGCTTC AGTTGCCTGG CTGCCCCACC CCCGTGTCTT TCGGCCTCCT CTTTTCTGTG
    GCCTTCCCCG TGGATGGAGA GCCTGATGCA GAGGTTGTGG TGGGAACCAC GAGGCCAGAG
    ACGCTGCCTG GAGACGTGGC TGTGGCGGTC CATCCTGATG ATTCCCGCTA CACGCATCTA
    CATGGGCGAC AACTCTGTCA TCCCTTGACG GGGCAGCTTC TCCCTCTTAT CACAGACTGC
    ACTGTTCAGC CACACCTGGG CACAGGGGCG GTGAAGGTGA CTCCAGCGCA CAGCCCTGCC
    GATGCTGAGC TGGGGGCCCG ACACGGCCTG AGCCCCCGGA GTGTCATTGC GGAGGATGGG
    ACCATGACCG CCCTTTGTGA GGACTGGCTG CAGGGTCTTC ACCGATTTGT GGCCCGGGAA
    AAGATTCTGT CAGCACTGAG GGAGCGGGGC CTGTTCCGGG GCCTCCAGAA CCACCCCATG
    GTGCTGCCCA TTTGCAGCCG TTCCGGGGAC GTGGTAGAGT ACCTACTGAA GAGCCAGTGG
    TTTGTCCGCT GCCGGGAAAT GGGGGAGCGA GCCGCCAAGG CCGTGGAGTC GGGGGCCCTG
    GACCTCAGTC CCTCCTTCCA CCAGAAGAAC TGGCAGCACT GGTTTTCCCA CATTGGGGAC
    TGGTGTGTCT CCCGGCAGCT GTGGTGGGGC CATCGGATTC CAGCCTACCT GGTTGTAGAG
    GAACATGCAA AGGGTGAAAC GGAGGACTTC TGGGTGGTCG GGCGGACAGA GGCTGAGGCC
    AGAGAAGTAG CTGCAGAATT AACAGGGAGA CCAGGGGCGG AGCTGACCCT GGAGAGGGAC
    CCTGATGTCC TGGACACGTG GTTCTCCTCC GCTCTTTTCC CCTTTTCTGC CCTGGGCTGG
    CCCCGAGAGA CCCCGGACCT GGCTCGTTTC TACCCCCTGT CACTTTTGGA AACGGGCAGT
    GACCTCCTGC TCTTCTGGGT GGGCCGCATG GTCATGTTGG GGACCCAGCT CACAGGGCAG
    CTCCCCTTCA GCAAGGTCCT GCTTCACTCC ATGGTCCGGG ACAGGCAGGG CCGGAAGATG
    AGCAAGTCCC TGGGGAACGT GCTGGACCCA CGGGACATCA TCAGTGGGGT GGAGCTGCAG
    GTACTGCAGG ACAATCTGAG GGATGGAAAC TTGGACCCCG CAGAGCTGGC GATTGCAGCC
    TCAGCGCAGA GAAAGGACTT CCCTCATGGG ATCCCCGAGT GTGGGACAGA TGCTCTGAGG
    TTCACCCTGT GCTCCCATGG GGCCCTGGGA GGTGACTTGC ACCTGTCCGT CTCTGAGGTC
    CTGAGCTTCC GACACTTCTG CAATAAGATC TGGAATGCCC TGCGCTTTAT CCTGAATGCT
    CTTGGGGAGG AATTCATACC CCAACCTGCG GAGGAGCTGT CCCCCGCCTC CCGCATGGAC
    ACCTGGATCC TCAGCTGCCT GGCCCGCACG GCCCAGGACT GCGAGCGGGG CTTCCTCACC
    CGCGAGCTCT CGCTCGTCAC CCACGCCCTG CACCACTTCT GGCTGCACAA CCTCTGTGAT
    GTCTACTTGG AGGCTGTGAA GCCGGTGCTA TCACACTCTC CCCGCCCTCT GGAGCCGCCT
    CAGGTCCTGT TCTTCTGCGC AGACGTCGGT CTCCGCCTCC TCGCCCCGTT GATGCCTTTC
    CTGGCTGAAG AGCTCTGGCA GAGGCTGCCC CCCAGGCCTG GTGGTCCCTC TGCCCCCAGC
    ATCTGTGTTG CCCCCTACCC AAGTGCCCGC AGCTTGGAGC ACTGGCACCA GCCTGAGCTG
    GAGAGGCGCT TCTCCCGGGT TCAGGAGTCC GTGCAGGTGC TCAGGGCTCT GCGCGCCACC
    TACCAGCTCA CCAAGGCCCG GCCCCGAGTG CTGCTGCAGA GCTCAGAGCC AGGCGAGCAG
    GGCCTCTTTG AGGCCTTCCT GGAGCCGCTG GGCATCCTGG GCTACTGTGG GGCGGTGGGC
    CTTCTACCCC CTGGTGCAGC AGCCCCCTCA GGCTGGGTCC AGGCCGCCCT CAGTGACACC
    AGTCAGGCGT ATATGGAGCT GCAGGGCCTG GTGGACCCCC AGACCCACCT ACCTCGGCTG
    GCTGCCCGAA GACACAAGTT GCAGAAGCAG CTTGATGGCC TCCTAGCCCG GCCCCCATCC
    GAGGGACAGG CGGAGACTCA GAGGCAGCAG AGGCTTTCTT CCCTCCAGTT GGAGTTGTCA
    AAACTGGACA AGGCGGCCTC TCACCTCCAG CAGCTGATGG ATGATTTTCC AAGCCCTGGG
    AGCTCACCAT CCCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005223639.2
    CDS230..3307
    Translation

    Target ORF information:

    RefSeq Version XM_005223582.3
    Organism Bos taurus(cattle)
    Definition Bos taurus valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005223582.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCCTCATT TACCTCTGAC CTCTTTTCGA CCACCGCTTT GGGGCTTGAG GCCCTCACGG 
    GGCCTCCCCA GGTCCCGTCC CCTTTCCACC CAGTCAGAGC CAAATGGATC AGCCATCTCC
    CGGAGGAACC GTGAAGCCAA ACAGAAGCGC CTGCGAGAGA AGCAGGCATC GCTGGAGGCT
    GGGATAGCCC CCAAGAGCAA GTCACCTGCA GAATCCAGTA AGGCCTGGAC CCCTAAGGAG
    AGAGTGTTGT ATGAAATCCC CACGGAACAT GGAGAAAAGA AAGATGTCTC CCGACCCCTG
    CCTCCTGCAT ACAGCCCCCA GTACGTTGAG GCTGCCTGGT ACCCTTGGTG GGTGCGAGAG
    GGCTTCTTCA AACCAGAATA TCAGGCCAGG CTGCCCCAGG CCACAGGGGA GACCTTTTCC
    ATGTGTATCC CACCCCCTAA TGTCACAGGC TCCCTGCACA TTGGGCATGC GCTGACCGTG
    GCCATACAGG ATGCTCTCGT GCGCTGGCAC CGGATGCGTG GGGATCGGGT GCTGTGGGTC
    CCTGGCTCAG ATCATGCAGG GATTGCAACC CAAGCTGTGG TGGAGAAACA GCTGTGGAAG
    GAGCGAGGCG TGAGGAGACA TGAGCTGAGC CGGGAAGACT TCCTTAGGGA GGTGTGGAAG
    TGGAAGGAGG AGAAAGGTGG CGAGATCTGT GAGCAGCTCC GAGCGCTGGG GGCCTCCCTG
    GACTGGGACC GAGAGTGCTT TACCATGGAT ACCGTGGAGA GCCGGCCCCT CCCTGGCCGC
    ACGGAGCTTC AGTTGCCTGG CTGCCCCACC CCCGTGTCTT TCGGCCTCCT CTTTTCTGTG
    GCCTTCCCCG TGGATGGAGA GCCTGATGCA GAGGTTGTGG TGGGAACCAC GAGGCCAGAG
    ACGCTGCCTG GAGACGTGGC TGTGGCGGTC CATCCTGATG ATTCCCGCTA CACGCATCTA
    CATGGGCGAC AACTCTGTCA TCCCTTGACG GGGCAGCTTC TCCCTCTTAT CACAGACTGC
    ACTGTTCAGC CACACCTGGG CACAGGGGCG GTGAAGGTGA CTCCAGCGCA CAGCCCTGCC
    GATGCTGAGC TGGGGGCCCG ACACGGCCTG AGCCCCCGGA GTGTCATTGC GGAGGATGGG
    ACCATGACCG CCCTTTGTGA GGACTGGCTG CAGGGTCTTC ACCGATTTGT GGCCCGGGAA
    AAGATTCTGT CAGCACTGAG GGAGCGGGGC CTGTTCCGGG GCCTCCAGAA CCACCCCATG
    GTGCTGCCCA TTTGCAGCCG TTCCGGGGAC GTGGTAGAGT ACCTACTGAA GAGCCAGTGG
    TTTGTCCGCT GCCGGGAAAT GGGGGAGCGA GCCGCCAAGG CCGTGGAGTC GGGGGCCCTG
    GACCTCAGTC CCTCCTTCCA CCAGAAGAAC TGGCAGCACT GGTTTTCCCA CATTGGGGAC
    TGGTGTGTCT CCCGGCAGCT GTGGTGGGGC CATCGGATTC CAGCCTACCT GGTTGTAGAG
    GAACATGCAA AGGGTGAAAC GGAGGACTTC TGGGTGGTCG GGCGGACAGA GGCTGAGGCC
    AGAGAAGTAG CTGCAGAATT AACAGGGAGA CCAGGGGCGG AGCTGACCCT GGAGAGGGAC
    CCTGATGTCC TGGACACGTG GTTCTCCTCC GCTCTTTTCC CCTTTTCTGC CCTGGGCTGG
    CCCCGAGAGA CCCCGGACCT GGCTCGTTTC TACCCCCTGT CACTTTTGGA AACGGGCAGT
    GACCTCCTGC TCTTCTGGGT GGGCCGCATG GTCATGTTGG GGACCCAGCT CACAGGGCAG
    CTCCCCTTCA GCAAGGTCCT GCTTCACTCC ATGGTCCGGG ACAGGCAGGG CCGGAAGATG
    AGCAAGTCCC TGGGGAACGT GCTGGACCCA CGGGACATCA TCAGTGGGGT GGAGCTGCAG
    GTACTGCAGG ACAATCTGAG GGATGGAAAC TTGGACCCCG CAGAGCTGGC GATTGCAGCC
    TCAGCGCAGA GAAAGGACTT CCCTCATGGG ATCCCCGAGT GTGGGACAGA TGCTCTGAGG
    TTCACCCTGT GCTCCCATGG GGCCCTGGGA GGTGACTTGC ACCTGTCCGT CTCTGAGGTC
    CTGAGCTTCC GACACTTCTG CAATAAGATC TGGAATGCCC TGCGCTTTAT CCTGAATGCT
    CTTGGGGAGG AATTCATACC CCAACCTGCG GAGGAGCTGT CCCCCGCCTC CCGCATGGAC
    ACCTGGATCC TCAGCTGCCT GGCCCGCACG GCCCAGGACT GCGAGCGGGG CTTCCTCACC
    CGCGAGCTCT CGCTCGTCAC CCACGCCCTG CACCACTTCT GGCTGCACAA CCTCTGTGAT
    GTCTACTTGG AGGCTGTGAA GCCGGTGCTA TCACACTCTC CCCGCCCTCT GGAGCCGCCT
    CAGGTCCTGT TCTTCTGCGC AGACGTCGGT CTCCGCCTCC TCGCCCCGTT GATGCCTTTC
    CTGGCTGAAG AGCTCTGGCA GAGGCTGCCC CCCAGGCCTG GTGGTCCCTC TGCCCCCAGC
    ATCTGTGTTG CCCCCTACCC AAGTGCCCGC AGCTTGGAGC ACTGGCACCA GCCTGAGCTG
    GAGAGGCGCT TCTCCCGGGT TCAGGAGTCC GTGCAGGTGC TCAGGGCTCT GCGCGCCACC
    TACCAGCTCA CCAAGGCCCG GCCCCGAGTG CTGCTGCAGA GCTCAGAGCC AGGCGAGCAG
    GGCCTCTTTG AGGCCTTCCT GGAGCCGCTG GGCATCCTGG GCTACTGTGG GGCGGTGGGC
    CTTCTACCCC CTGGTGCAGC AGCCCCCTCA GGCTGGGTCC AGGCCGCCCT CAGTGACACC
    AGTCAGGCGT ATATGGAGCT GCAGGGCCTG GTGGACCCCC AGACCCACCT ACCTCGGCTG
    GCTGCCCGAA GACACAAGTT GCAGAAGCAG CTTGATGGCC TCCTAGCCCG GCCCCCATCC
    GAGGGACAGG CGGAGACTCA GAGGCAGCAG AGGCTTTCTT CCCTCCAGTT GGAGTTGTCA
    AAACTGGACA AGGCGGCCTC TCACCTCCAG CAGCTGATGG ATGATTTTCC AAGCCCTGGG
    AGCTCACCAT CCCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa60389
    Clone ID Related Accession (Same CDS sequence) XM_005223582.3 , XM_005223582.4
    Accession Version XM_005223582.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3078bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product valine--tRNA ligase, mitochondrial isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005223582.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCCTCATT TACCTCTGAC CTCTTTTCGA CCACCGCTTT GGGGCTTGAG GCCCTCACGG 
    GGCCTCCCCA GGTCCCGTCC CCTTTCCACC CAGTCAGAGC CAAATGGATC AGCCATCTCC
    CGGAGGAACC GTGAAGCCAA ACAGAAGCGC CTGCGAGAGA AGCAGGCATC GCTGGAGGCT
    GGGATAGCCC CCAAGAGCAA GTCACCTGCA GAATCCAGTA AGGCCTGGAC CCCTAAGGAG
    AGAGTGTTGT ATGAAATCCC CACGGAACAT GGAGAAAAGA AAGATGTCTC CCGACCCCTG
    CCTCCTGCAT ACAGCCCCCA GTACGTTGAG GCTGCCTGGT ACCCTTGGTG GGTGCGAGAG
    GGCTTCTTCA AACCAGAATA TCAGGCCAGG CTGCCCCAGG CCACAGGGGA GACCTTTTCC
    ATGTGTATCC CACCCCCTAA TGTCACAGGC TCCCTGCACA TTGGGCATGC GCTGACCGTG
    GCCATACAGG ATGCTCTCGT GCGCTGGCAC CGGATGCGTG GGGATCGGGT GCTGTGGGTC
    CCTGGCTCAG ATCATGCAGG GATTGCAACC CAAGCTGTGG TGGAGAAACA GCTGTGGAAG
    GAGCGAGGCG TGAGGAGACA TGAGCTGAGC CGGGAAGACT TCCTTAGGGA GGTGTGGAAG
    TGGAAGGAGG AGAAAGGTGG CGAGATCTGT GAGCAGCTCC GAGCGCTGGG GGCCTCCCTG
    GACTGGGACC GAGAGTGCTT TACCATGGAT ACCGTGGAGA GCCGGCCCCT CCCTGGCCGC
    ACGGAGCTTC AGTTGCCTGG CTGCCCCACC CCCGTGTCTT TCGGCCTCCT CTTTTCTGTG
    GCCTTCCCCG TGGATGGAGA GCCTGATGCA GAGGTTGTGG TGGGAACCAC GAGGCCAGAG
    ACGCTGCCTG GAGACGTGGC TGTGGCGGTC CATCCTGATG ATTCCCGCTA CACGCATCTA
    CATGGGCGAC AACTCTGTCA TCCCTTGACG GGGCAGCTTC TCCCTCTTAT CACAGACTGC
    ACTGTTCAGC CACACCTGGG CACAGGGGCG GTGAAGGTGA CTCCAGCGCA CAGCCCTGCC
    GATGCTGAGC TGGGGGCCCG ACACGGCCTG AGCCCCCGGA GTGTCATTGC GGAGGATGGG
    ACCATGACCG CCCTTTGTGA GGACTGGCTG CAGGGTCTTC ACCGATTTGT GGCCCGGGAA
    AAGATTCTGT CAGCACTGAG GGAGCGGGGC CTGTTCCGGG GCCTCCAGAA CCACCCCATG
    GTGCTGCCCA TTTGCAGCCG TTCCGGGGAC GTGGTAGAGT ACCTACTGAA GAGCCAGTGG
    TTTGTCCGCT GCCGGGAAAT GGGGGAGCGA GCCGCCAAGG CCGTGGAGTC GGGGGCCCTG
    GACCTCAGTC CCTCCTTCCA CCAGAAGAAC TGGCAGCACT GGTTTTCCCA CATTGGGGAC
    TGGTGTGTCT CCCGGCAGCT GTGGTGGGGC CATCGGATTC CAGCCTACCT GGTTGTAGAG
    GAACATGCAA AGGGTGAAAC GGAGGACTTC TGGGTGGTCG GGCGGACAGA GGCTGAGGCC
    AGAGAAGTAG CTGCAGAATT AACAGGGAGA CCAGGGGCGG AGCTGACCCT GGAGAGGGAC
    CCTGATGTCC TGGACACGTG GTTCTCCTCC GCTCTTTTCC CCTTTTCTGC CCTGGGCTGG
    CCCCGAGAGA CCCCGGACCT GGCTCGTTTC TACCCCCTGT CACTTTTGGA AACGGGCAGT
    GACCTCCTGC TCTTCTGGGT GGGCCGCATG GTCATGTTGG GGACCCAGCT CACAGGGCAG
    CTCCCCTTCA GCAAGGTCCT GCTTCACTCC ATGGTCCGGG ACAGGCAGGG CCGGAAGATG
    AGCAAGTCCC TGGGGAACGT GCTGGACCCA CGGGACATCA TCAGTGGGGT GGAGCTGCAG
    GTACTGCAGG ACAATCTGAG GGATGGAAAC TTGGACCCCG CAGAGCTGGC GATTGCAGCC
    TCAGCGCAGA GAAAGGACTT CCCTCATGGG ATCCCCGAGT GTGGGACAGA TGCTCTGAGG
    TTCACCCTGT GCTCCCATGG GGCCCTGGGA GGTGACTTGC ACCTGTCCGT CTCTGAGGTC
    CTGAGCTTCC GACACTTCTG CAATAAGATC TGGAATGCCC TGCGCTTTAT CCTGAATGCT
    CTTGGGGAGG AATTCATACC CCAACCTGCG GAGGAGCTGT CCCCCGCCTC CCGCATGGAC
    ACCTGGATCC TCAGCTGCCT GGCCCGCACG GCCCAGGACT GCGAGCGGGG CTTCCTCACC
    CGCGAGCTCT CGCTCGTCAC CCACGCCCTG CACCACTTCT GGCTGCACAA CCTCTGTGAT
    GTCTACTTGG AGGCTGTGAA GCCGGTGCTA TCACACTCTC CCCGCCCTCT GGAGCCGCCT
    CAGGTCCTGT TCTTCTGCGC AGACGTCGGT CTCCGCCTCC TCGCCCCGTT GATGCCTTTC
    CTGGCTGAAG AGCTCTGGCA GAGGCTGCCC CCCAGGCCTG GTGGTCCCTC TGCCCCCAGC
    ATCTGTGTTG CCCCCTACCC AAGTGCCCGC AGCTTGGAGC ACTGGCACCA GCCTGAGCTG
    GAGAGGCGCT TCTCCCGGGT TCAGGAGTCC GTGCAGGTGC TCAGGGCTCT GCGCGCCACC
    TACCAGCTCA CCAAGGCCCG GCCCCGAGTG CTGCTGCAGA GCTCAGAGCC AGGCGAGCAG
    GGCCTCTTTG AGGCCTTCCT GGAGCCGCTG GGCATCCTGG GCTACTGTGG GGCGGTGGGC
    CTTCTACCCC CTGGTGCAGC AGCCCCCTCA GGCTGGGTCC AGGCCGCCCT CAGTGACACC
    AGTCAGGCGT ATATGGAGCT GCAGGGCCTG GTGGACCCCC AGACCCACCT ACCTCGGCTG
    GCTGCCCGAA GACACAAGTT GCAGAAGCAG CTTGATGGCC TCCTAGCCCG GCCCCCATCC
    GAGGGACAGG CGGAGACTCA GAGGCAGCAG AGGCTTTCTT CCCTCCAGTT GGAGTTGTCA
    AAACTGGACA AGGCGGCCTC TCACCTCCAG CAGCTGATGG ATGATTTTCC AAGCCCTGGG
    AGCTCACCAT CCCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005223639.2
    CDS223..3300
    Translation

    Target ORF information:

    RefSeq Version XM_005223582.4
    Organism Bos taurus(cattle)
    Definition Bos taurus valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005223582.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCCTCATT TACCTCTGAC CTCTTTTCGA CCACCGCTTT GGGGCTTGAG GCCCTCACGG 
    GGCCTCCCCA GGTCCCGTCC CCTTTCCACC CAGTCAGAGC CAAATGGATC AGCCATCTCC
    CGGAGGAACC GTGAAGCCAA ACAGAAGCGC CTGCGAGAGA AGCAGGCATC GCTGGAGGCT
    GGGATAGCCC CCAAGAGCAA GTCACCTGCA GAATCCAGTA AGGCCTGGAC CCCTAAGGAG
    AGAGTGTTGT ATGAAATCCC CACGGAACAT GGAGAAAAGA AAGATGTCTC CCGACCCCTG
    CCTCCTGCAT ACAGCCCCCA GTACGTTGAG GCTGCCTGGT ACCCTTGGTG GGTGCGAGAG
    GGCTTCTTCA AACCAGAATA TCAGGCCAGG CTGCCCCAGG CCACAGGGGA GACCTTTTCC
    ATGTGTATCC CACCCCCTAA TGTCACAGGC TCCCTGCACA TTGGGCATGC GCTGACCGTG
    GCCATACAGG ATGCTCTCGT GCGCTGGCAC CGGATGCGTG GGGATCGGGT GCTGTGGGTC
    CCTGGCTCAG ATCATGCAGG GATTGCAACC CAAGCTGTGG TGGAGAAACA GCTGTGGAAG
    GAGCGAGGCG TGAGGAGACA TGAGCTGAGC CGGGAAGACT TCCTTAGGGA GGTGTGGAAG
    TGGAAGGAGG AGAAAGGTGG CGAGATCTGT GAGCAGCTCC GAGCGCTGGG GGCCTCCCTG
    GACTGGGACC GAGAGTGCTT TACCATGGAT ACCGTGGAGA GCCGGCCCCT CCCTGGCCGC
    ACGGAGCTTC AGTTGCCTGG CTGCCCCACC CCCGTGTCTT TCGGCCTCCT CTTTTCTGTG
    GCCTTCCCCG TGGATGGAGA GCCTGATGCA GAGGTTGTGG TGGGAACCAC GAGGCCAGAG
    ACGCTGCCTG GAGACGTGGC TGTGGCGGTC CATCCTGATG ATTCCCGCTA CACGCATCTA
    CATGGGCGAC AACTCTGTCA TCCCTTGACG GGGCAGCTTC TCCCTCTTAT CACAGACTGC
    ACTGTTCAGC CACACCTGGG CACAGGGGCG GTGAAGGTGA CTCCAGCGCA CAGCCCTGCC
    GATGCTGAGC TGGGGGCCCG ACACGGCCTG AGCCCCCGGA GTGTCATTGC GGAGGATGGG
    ACCATGACCG CCCTTTGTGA GGACTGGCTG CAGGGTCTTC ACCGATTTGT GGCCCGGGAA
    AAGATTCTGT CAGCACTGAG GGAGCGGGGC CTGTTCCGGG GCCTCCAGAA CCACCCCATG
    GTGCTGCCCA TTTGCAGCCG TTCCGGGGAC GTGGTAGAGT ACCTACTGAA GAGCCAGTGG
    TTTGTCCGCT GCCGGGAAAT GGGGGAGCGA GCCGCCAAGG CCGTGGAGTC GGGGGCCCTG
    GACCTCAGTC CCTCCTTCCA CCAGAAGAAC TGGCAGCACT GGTTTTCCCA CATTGGGGAC
    TGGTGTGTCT CCCGGCAGCT GTGGTGGGGC CATCGGATTC CAGCCTACCT GGTTGTAGAG
    GAACATGCAA AGGGTGAAAC GGAGGACTTC TGGGTGGTCG GGCGGACAGA GGCTGAGGCC
    AGAGAAGTAG CTGCAGAATT AACAGGGAGA CCAGGGGCGG AGCTGACCCT GGAGAGGGAC
    CCTGATGTCC TGGACACGTG GTTCTCCTCC GCTCTTTTCC CCTTTTCTGC CCTGGGCTGG
    CCCCGAGAGA CCCCGGACCT GGCTCGTTTC TACCCCCTGT CACTTTTGGA AACGGGCAGT
    GACCTCCTGC TCTTCTGGGT GGGCCGCATG GTCATGTTGG GGACCCAGCT CACAGGGCAG
    CTCCCCTTCA GCAAGGTCCT GCTTCACTCC ATGGTCCGGG ACAGGCAGGG CCGGAAGATG
    AGCAAGTCCC TGGGGAACGT GCTGGACCCA CGGGACATCA TCAGTGGGGT GGAGCTGCAG
    GTACTGCAGG ACAATCTGAG GGATGGAAAC TTGGACCCCG CAGAGCTGGC GATTGCAGCC
    TCAGCGCAGA GAAAGGACTT CCCTCATGGG ATCCCCGAGT GTGGGACAGA TGCTCTGAGG
    TTCACCCTGT GCTCCCATGG GGCCCTGGGA GGTGACTTGC ACCTGTCCGT CTCTGAGGTC
    CTGAGCTTCC GACACTTCTG CAATAAGATC TGGAATGCCC TGCGCTTTAT CCTGAATGCT
    CTTGGGGAGG AATTCATACC CCAACCTGCG GAGGAGCTGT CCCCCGCCTC CCGCATGGAC
    ACCTGGATCC TCAGCTGCCT GGCCCGCACG GCCCAGGACT GCGAGCGGGG CTTCCTCACC
    CGCGAGCTCT CGCTCGTCAC CCACGCCCTG CACCACTTCT GGCTGCACAA CCTCTGTGAT
    GTCTACTTGG AGGCTGTGAA GCCGGTGCTA TCACACTCTC CCCGCCCTCT GGAGCCGCCT
    CAGGTCCTGT TCTTCTGCGC AGACGTCGGT CTCCGCCTCC TCGCCCCGTT GATGCCTTTC
    CTGGCTGAAG AGCTCTGGCA GAGGCTGCCC CCCAGGCCTG GTGGTCCCTC TGCCCCCAGC
    ATCTGTGTTG CCCCCTACCC AAGTGCCCGC AGCTTGGAGC ACTGGCACCA GCCTGAGCTG
    GAGAGGCGCT TCTCCCGGGT TCAGGAGTCC GTGCAGGTGC TCAGGGCTCT GCGCGCCACC
    TACCAGCTCA CCAAGGCCCG GCCCCGAGTG CTGCTGCAGA GCTCAGAGCC AGGCGAGCAG
    GGCCTCTTTG AGGCCTTCCT GGAGCCGCTG GGCATCCTGG GCTACTGTGG GGCGGTGGGC
    CTTCTACCCC CTGGTGCAGC AGCCCCCTCA GGCTGGGTCC AGGCCGCCCT CAGTGACACC
    AGTCAGGCGT ATATGGAGCT GCAGGGCCTG GTGGACCCCC AGACCCACCT ACCTCGGCTG
    GCTGCCCGAA GACACAAGTT GCAGAAGCAG CTTGATGGCC TCCTAGCCCG GCCCCCATCC
    GAGGGACAGG CGGAGACTCA GAGGCAGCAG AGGCTTTCTT CCCTCCAGTT GGAGTTGTCA
    AAACTGGACA AGGCGGCCTC TCACCTCCAG CAGCTGATGG ATGATTTTCC AAGCCCTGGG
    AGCTCACCAT CCCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.