VARS2 cDNA ORF clone, Canis lupus familiaris(dog)

The following VARS2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VARS2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe39501 XM_014118303.2
Latest version!
Canis lupus familiaris valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OCe292300 XM_022425817.1
Latest version!
Canis lupus familiaris valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe39497 XM_014118299.2
Latest version!
Canis lupus familiaris valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe39498 XM_014118300.2
Latest version!
Canis lupus familiaris valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe39499 XM_014118301.2
Latest version!
Canis lupus familiaris valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe39500 XM_014118302.2
Latest version!
Canis lupus familiaris valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe14991 XM_005627121.3
Latest version!
Canis lupus familiaris valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
OCe39500 XM_014118302.1 Canis lupus familiaris valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe39498 XM_014118300.1 Canis lupus familiaris valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe39501 XM_014118303.1 Canis lupus familiaris valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OCe14991 XM_005627121.2 Canis lupus familiaris valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OCe39499 XM_014118301.1 Canis lupus familiaris valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe39497 XM_014118299.1 Canis lupus familiaris valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe39501
    Clone ID Related Accession (Same CDS sequence) XM_014118303.2 , XM_014118303.1
    Accession Version XM_014118303.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2610bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product valine--tRNA ligase, mitochondrial isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006594.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014118303.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCTGCGGG TCTGGAGCGC CTCTCCGCCT TTGCAGACAC TCCTGATGCC TCGTTCGCCC 
    TTGGCCTCGC TTCCATCACG GTGCTGGGGG CTCGGGCCCT CTCAGGCCCT CCGCAGGTCC
    CACCCTCTCT CTACCCAGTC AGAGCCGTGC CGGTCCTCCC TCTCCCGGAG GAACCATGAA
    GCCAAACAGA AGCGCCAGCG GGAGAAGCAG ATGGCGCTGG AGGCTGGGAT CACCCCGAAG
    GGCAAGGCAC CTGCAGGATC CAGTAAGGCC TGGACTCCTA AGGAGGTAGT GTTGTATGAA
    GTCCCCACGG AACCGGGTGA AAAGAAAGAT GTGTACCGCC TCCTGCCTCC TGCATACAGT
    CCTCGCTACG TTGAGGCGGC CTGGTACTCC TGGTGGGTGC GAGAGGGCTT CTTCAAACCG
    GAGTATCAGA CCAGGCTGCC CCAGGCCACA GGGGAGACGT TTTCCATGTG TATCCCACCT
    CCCAATGTCA CTGGCTCCCT GCATATCGGT CATGCACTGA CAGTAGCCAT ACAGGATGCC
    CTGGTGCGCT GGCACCGGAT GCGTGGGGAT CAGGTGCTAT GGATCCCTGG CTCAGATCAT
    GCAGGAATTG CTACACAAGC TGTGGTGGAG AAGCAACTGT GGAAGGAGCG AGGAATGAGG
    AGACATGAAC TGAGCCGGGA AAAATTCCTT AGGGAGGTGT GGAAGTGGAA GGACGAGAAA
    GGTGGAGAGA TCAGTGAGCA GCTCCAAGCT CTGGGGGCCT CCCTGGACTG GGACCGAGAG
    TGCTTCACTA TGGATGCTGG CTTCTCAGTG GCCGTGACCG AAGCTTTTGT GCGACTCTAC
    GAGGCAGGAT TGTTGTACCG GAGCCAGCAG CTTGTCAACT GGTCCTGCAC TTTAAGATCA
    GCCATCTCGG ATATTGAGGT GGAGAGCCGG CCCCTGCCTG GCCACACGGA GCTTCAACTG
    CCTGGCTGCC CCACTCCTGT GTCTTTTGGC CTCCTTGTGT CTGTGGCCTT CCCTGTGGAT
    GGAGAGCCTG ATGCAGAGGT CGTGGTAGGA ACTACAAGGC CAGAGACGTT GCCTGGAGAC
    GTTGCTGTGG CTGTTCATCC CGACGACTCC CGATACACAC ACCTGCATGG GCGACAGCTT
    CGTCACCCCT TGACTGGGCA GCTTCTCCCC CTCATCACAG ACTCCACTGT TCAGCCACAT
    GTGGGCACAG GGGCAGTGAA GGTGACTCCA GCGCATAGCC CTGCTGATGC TGAGCTGGGG
    GCCCGACACG GCTTGAGCCC TCTGAGTGTC ATCGCGGAGG ATGGCACCAT GACCTCTCTC
    TGTGGGGACT GGCTACAGGG TCTTCACCGA TTTGTGGCCC GGGAAAAGAT TATATCTGCG
    CTGCGGGAGC GGGGCCTGTT CCGGGGCCTC CAGAACCACC CCATGGTGCT TCCTATTTGC
    AGCCGTTCTG GGGACGTGAT AGAATATCTA CTGAAGAGCC AGTGGTTTGT CCGCTGCCGG
    GAGATGGGGG ACCGAGCTGC CCAGATGCAA GTGAGGTGCA GGAAGGGAAG CTCTAACGGC
    CTTTCTGTTC AGGCCGTGGA GTCAGGGGCT CTGGAGCTCA GTCCATCCTT CCACCAGAAG
    AACTGGCAGC ACTGGTTTTC CCACATCGGG GACTGGTGTG TCTCCCGGCA GCTGTGGTGG
    GGCCATCGGA TTCCCGCCTA CCTGGTTGTA GAGGAGCACA TGAAGGGTGA TGGAGAAGAC
    TGTTGGGTGG TTGGGCGGAC AGAGACCGAG GCCAGAAAAA TAGCTGCAGA ACTGACTGGG
    AGATCAGAGG CAGAGCTGAC CCTGCAGAGG GATCCTGATG TCCTGGACAC ATGGTTCTCT
    TCGGCTCTTT TCCCCTTTGC TGCCTTGGGC TGGCCCCAAG AGACCCCAGA CCTTGCTCGT
    TTCTACCCCC TGTCACTTTT GGAAACAGGC AGTGATCTCC TGTTGTTCTG GGTGGGCCGC
    ATGGTCATGT TGGGGACACA GCTCACAGGG CAGCTCCCCT TCACCAAGGT GCTTCTTCAT
    TCCATGGTTC GGGACGGGCA GGGCCGGAAG ATGAGCAAGT CCCTGGGGAA TGTATTGGAC
    CCACGAGACA TCATCAGTGG GGTGGAACTG CAGGTGCTGC AGGAAAAGCT GAAGGACGGG
    AACTTGGACT CCACAGAGCT GGCGATTGCG GCTGCGGCAC AGCTGTGTAG GCATCTGCCA
    CCCTTCTTAT CCCTCTGGTT GCAGAGGAAG GACTTCCCTC ATGGGATCCC TGAGTGTGGG
    ACAGATGCCC TGCGATTTGC CCTGTGCTCC CACAGGGCCT TGGGGGGCGA TGTACACTTG
    TCCGTCTCTG AGGTCTTGAG TTCCCGGCAT TTCTGCAACA AAATCTGGAA TGCCCTGCGT
    TTTATCCTCA ATGCCTTAGG AGAGGGATTT GTACCGCAGC CTGCAGAGGA GCTATCCCCC
    TCATCCCCCA CGGACGCCTG GATCCTGAGC CGTCTGGCCC ACACTGCCCA GGAGTGTGAG
    CGAGCCTTCC TTGCTCGGGA GCTGCCACTC ATCACCCATG CCCTCCACCA CTTCTGGCTC
    CACAGCCTCT GTGATGTCTA CTTGCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013973778.1
    CDS106..2715
    Translation

    Target ORF information:

    RefSeq Version XM_014118303.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014118303.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCTGCGGG TCTGGAGCGC CTCTCCGCCT TTGCAGACAC TCCTGATGCC TCGTTCGCCC 
    TTGGCCTCGC TTCCATCACG GTGCTGGGGG CTCGGGCCCT CTCAGGCCCT CCGCAGGTCC
    CACCCTCTCT CTACCCAGTC AGAGCCGTGC CGGTCCTCCC TCTCCCGGAG GAACCATGAA
    GCCAAACAGA AGCGCCAGCG GGAGAAGCAG ATGGCGCTGG AGGCTGGGAT CACCCCGAAG
    GGCAAGGCAC CTGCAGGATC CAGTAAGGCC TGGACTCCTA AGGAGGTAGT GTTGTATGAA
    GTCCCCACGG AACCGGGTGA AAAGAAAGAT GTGTACCGCC TCCTGCCTCC TGCATACAGT
    CCTCGCTACG TTGAGGCGGC CTGGTACTCC TGGTGGGTGC GAGAGGGCTT CTTCAAACCG
    GAGTATCAGA CCAGGCTGCC CCAGGCCACA GGGGAGACGT TTTCCATGTG TATCCCACCT
    CCCAATGTCA CTGGCTCCCT GCATATCGGT CATGCACTGA CAGTAGCCAT ACAGGATGCC
    CTGGTGCGCT GGCACCGGAT GCGTGGGGAT CAGGTGCTAT GGATCCCTGG CTCAGATCAT
    GCAGGAATTG CTACACAAGC TGTGGTGGAG AAGCAACTGT GGAAGGAGCG AGGAATGAGG
    AGACATGAAC TGAGCCGGGA AAAATTCCTT AGGGAGGTGT GGAAGTGGAA GGACGAGAAA
    GGTGGAGAGA TCAGTGAGCA GCTCCAAGCT CTGGGGGCCT CCCTGGACTG GGACCGAGAG
    TGCTTCACTA TGGATGCTGG CTTCTCAGTG GCCGTGACCG AAGCTTTTGT GCGACTCTAC
    GAGGCAGGAT TGTTGTACCG GAGCCAGCAG CTTGTCAACT GGTCCTGCAC TTTAAGATCA
    GCCATCTCGG ATATTGAGGT GGAGAGCCGG CCCCTGCCTG GCCACACGGA GCTTCAACTG
    CCTGGCTGCC CCACTCCTGT GTCTTTTGGC CTCCTTGTGT CTGTGGCCTT CCCTGTGGAT
    GGAGAGCCTG ATGCAGAGGT CGTGGTAGGA ACTACAAGGC CAGAGACGTT GCCTGGAGAC
    GTTGCTGTGG CTGTTCATCC CGACGACTCC CGATACACAC ACCTGCATGG GCGACAGCTT
    CGTCACCCCT TGACTGGGCA GCTTCTCCCC CTCATCACAG ACTCCACTGT TCAGCCACAT
    GTGGGCACAG GGGCAGTGAA GGTGACTCCA GCGCATAGCC CTGCTGATGC TGAGCTGGGG
    GCCCGACACG GCTTGAGCCC TCTGAGTGTC ATCGCGGAGG ATGGCACCAT GACCTCTCTC
    TGTGGGGACT GGCTACAGGG TCTTCACCGA TTTGTGGCCC GGGAAAAGAT TATATCTGCG
    CTGCGGGAGC GGGGCCTGTT CCGGGGCCTC CAGAACCACC CCATGGTGCT TCCTATTTGC
    AGCCGTTCTG GGGACGTGAT AGAATATCTA CTGAAGAGCC AGTGGTTTGT CCGCTGCCGG
    GAGATGGGGG ACCGAGCTGC CCAGATGCAA GTGAGGTGCA GGAAGGGAAG CTCTAACGGC
    CTTTCTGTTC AGGCCGTGGA GTCAGGGGCT CTGGAGCTCA GTCCATCCTT CCACCAGAAG
    AACTGGCAGC ACTGGTTTTC CCACATCGGG GACTGGTGTG TCTCCCGGCA GCTGTGGTGG
    GGCCATCGGA TTCCCGCCTA CCTGGTTGTA GAGGAGCACA TGAAGGGTGA TGGAGAAGAC
    TGTTGGGTGG TTGGGCGGAC AGAGACCGAG GCCAGAAAAA TAGCTGCAGA ACTGACTGGG
    AGATCAGAGG CAGAGCTGAC CCTGCAGAGG GATCCTGATG TCCTGGACAC ATGGTTCTCT
    TCGGCTCTTT TCCCCTTTGC TGCCTTGGGC TGGCCCCAAG AGACCCCAGA CCTTGCTCGT
    TTCTACCCCC TGTCACTTTT GGAAACAGGC AGTGATCTCC TGTTGTTCTG GGTGGGCCGC
    ATGGTCATGT TGGGGACACA GCTCACAGGG CAGCTCCCCT TCACCAAGGT GCTTCTTCAT
    TCCATGGTTC GGGACGGGCA GGGCCGGAAG ATGAGCAAGT CCCTGGGGAA TGTATTGGAC
    CCACGAGACA TCATCAGTGG GGTGGAACTG CAGGTGCTGC AGGAAAAGCT GAAGGACGGG
    AACTTGGACT CCACAGAGCT GGCGATTGCG GCTGCGGCAC AGCTGTGTAG GCATCTGCCA
    CCCTTCTTAT CCCTCTGGTT GCAGAGGAAG GACTTCCCTC ATGGGATCCC TGAGTGTGGG
    ACAGATGCCC TGCGATTTGC CCTGTGCTCC CACAGGGCCT TGGGGGGCGA TGTACACTTG
    TCCGTCTCTG AGGTCTTGAG TTCCCGGCAT TTCTGCAACA AAATCTGGAA TGCCCTGCGT
    TTTATCCTCA ATGCCTTAGG AGAGGGATTT GTACCGCAGC CTGCAGAGGA GCTATCCCCC
    TCATCCCCCA CGGACGCCTG GATCCTGAGC CGTCTGGCCC ACACTGCCCA GGAGTGTGAG
    CGAGCCTTCC TTGCTCGGGA GCTGCCACTC ATCACCCATG CCCTCCACCA CTTCTGGCTC
    CACAGCCTCT GTGATGTCTA CTTGCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe39501
    Clone ID Related Accession (Same CDS sequence) XM_014118303.2 , XM_014118303.1
    Accession Version XM_014118303.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2610bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product valine--tRNA ligase, mitochondrial isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726081.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCTGCGGG TCTGGAGCGC CTCTCCGCCT TTGCAGACAC TCCTGATGCC TCGTTCGCCC 
    TTGGCCTCGC TTCCATCACG GTGCTGGGGG CTCGGGCCCT CTCAGGCCCT CCGCAGGTCC
    CACCCTCTCT CTACCCAGTC AGAGCCGTGC CGGTCCTCCC TCTCCCGGAG GAACCATGAA
    GCCAAACAGA AGCGCCAGCG GGAGAAGCAG ATGGCGCTGG AGGCTGGGAT CACCCCGAAG
    GGCAAGGCAC CTGCAGGATC CAGTAAGGCC TGGACTCCTA AGGAGGTAGT GTTGTATGAA
    GTCCCCACGG AACCGGGTGA AAAGAAAGAT GTGTACCGCC TCCTGCCTCC TGCATACAGT
    CCTCGCTACG TTGAGGCGGC CTGGTACTCC TGGTGGGTGC GAGAGGGCTT CTTCAAACCG
    GAGTATCAGA CCAGGCTGCC CCAGGCCACA GGGGAGACGT TTTCCATGTG TATCCCACCT
    CCCAATGTCA CTGGCTCCCT GCATATCGGT CATGCACTGA CAGTAGCCAT ACAGGATGCC
    CTGGTGCGCT GGCACCGGAT GCGTGGGGAT CAGGTGCTAT GGATCCCTGG CTCAGATCAT
    GCAGGAATTG CTACACAAGC TGTGGTGGAG AAGCAACTGT GGAAGGAGCG AGGAATGAGG
    AGACATGAAC TGAGCCGGGA AAAATTCCTT AGGGAGGTGT GGAAGTGGAA GGACGAGAAA
    GGTGGAGAGA TCAGTGAGCA GCTCCAAGCT CTGGGGGCCT CCCTGGACTG GGACCGAGAG
    TGCTTCACTA TGGATGCTGG CTTCTCAGTG GCCGTGACCG AAGCTTTTGT GCGACTCTAC
    GAGGCAGGAT TGTTGTACCG GAGCCAGCAG CTTGTCAACT GGTCCTGCAC TTTAAGATCA
    GCCATCTCGG ATATTGAGGT GGAGAGCCGG CCCCTGCCTG GCCACACGGA GCTTCAACTG
    CCTGGCTGCC CCACTCCTGT GTCTTTTGGC CTCCTTGTGT CTGTGGCCTT CCCTGTGGAT
    GGAGAGCCTG ATGCAGAGGT CGTGGTAGGA ACTACAAGGC CAGAGACGTT GCCTGGAGAC
    GTTGCTGTGG CTGTTCATCC CGACGACTCC CGATACACAC ACCTGCATGG GCGACAGCTT
    CGTCACCCCT TGACTGGGCA GCTTCTCCCC CTCATCACAG ACTCCACTGT TCAGCCACAT
    GTGGGCACAG GGGCAGTGAA GGTGACTCCA GCGCATAGCC CTGCTGATGC TGAGCTGGGG
    GCCCGACACG GCTTGAGCCC TCTGAGTGTC ATCGCGGAGG ATGGCACCAT GACCTCTCTC
    TGTGGGGACT GGCTACAGGG TCTTCACCGA TTTGTGGCCC GGGAAAAGAT TATATCTGCG
    CTGCGGGAGC GGGGCCTGTT CCGGGGCCTC CAGAACCACC CCATGGTGCT TCCTATTTGC
    AGCCGTTCTG GGGACGTGAT AGAATATCTA CTGAAGAGCC AGTGGTTTGT CCGCTGCCGG
    GAGATGGGGG ACCGAGCTGC CCAGATGCAA GTGAGGTGCA GGAAGGGAAG CTCTAACGGC
    CTTTCTGTTC AGGCCGTGGA GTCAGGGGCT CTGGAGCTCA GTCCATCCTT CCACCAGAAG
    AACTGGCAGC ACTGGTTTTC CCACATCGGG GACTGGTGTG TCTCCCGGCA GCTGTGGTGG
    GGCCATCGGA TTCCCGCCTA CCTGGTTGTA GAGGAGCACA TGAAGGGTGA TGGAGAAGAC
    TGTTGGGTGG TTGGGCGGAC AGAGACCGAG GCCAGAAAAA TAGCTGCAGA ACTGACTGGG
    AGATCAGAGG CAGAGCTGAC CCTGCAGAGG GATCCTGATG TCCTGGACAC ATGGTTCTCT
    TCGGCTCTTT TCCCCTTTGC TGCCTTGGGC TGGCCCCAAG AGACCCCAGA CCTTGCTCGT
    TTCTACCCCC TGTCACTTTT GGAAACAGGC AGTGATCTCC TGTTGTTCTG GGTGGGCCGC
    ATGGTCATGT TGGGGACACA GCTCACAGGG CAGCTCCCCT TCACCAAGGT GCTTCTTCAT
    TCCATGGTTC GGGACGGGCA GGGCCGGAAG ATGAGCAAGT CCCTGGGGAA TGTATTGGAC
    CCACGAGACA TCATCAGTGG GGTGGAACTG CAGGTGCTGC AGGAAAAGCT GAAGGACGGG
    AACTTGGACT CCACAGAGCT GGCGATTGCG GCTGCGGCAC AGCTGTGTAG GCATCTGCCA
    CCCTTCTTAT CCCTCTGGTT GCAGAGGAAG GACTTCCCTC ATGGGATCCC TGAGTGTGGG
    ACAGATGCCC TGCGATTTGC CCTGTGCTCC CACAGGGCCT TGGGGGGCGA TGTACACTTG
    TCCGTCTCTG AGGTCTTGAG TTCCCGGCAT TTCTGCAACA AAATCTGGAA TGCCCTGCGT
    TTTATCCTCA ATGCCTTAGG AGAGGGATTT GTACCGCAGC CTGCAGAGGA GCTATCCCCC
    TCATCCCCCA CGGACGCCTG GATCCTGAGC CGTCTGGCCC ACACTGCCCA GGAGTGTGAG
    CGAGCCTTCC TTGCTCGGGA GCTGCCACTC ATCACCCATG CCCTCCACCA CTTCTGGCTC
    CACAGCCTCT GTGATGTCTA CTTGCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013973778.1
    CDS131..2740
    Translation

    Target ORF information:

    RefSeq Version XM_014118303.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014118303.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCTGCGGG TCTGGAGCGC CTCTCCGCCT TTGCAGACAC TCCTGATGCC TCGTTCGCCC 
    TTGGCCTCGC TTCCATCACG GTGCTGGGGG CTCGGGCCCT CTCAGGCCCT CCGCAGGTCC
    CACCCTCTCT CTACCCAGTC AGAGCCGTGC CGGTCCTCCC TCTCCCGGAG GAACCATGAA
    GCCAAACAGA AGCGCCAGCG GGAGAAGCAG ATGGCGCTGG AGGCTGGGAT CACCCCGAAG
    GGCAAGGCAC CTGCAGGATC CAGTAAGGCC TGGACTCCTA AGGAGGTAGT GTTGTATGAA
    GTCCCCACGG AACCGGGTGA AAAGAAAGAT GTGTACCGCC TCCTGCCTCC TGCATACAGT
    CCTCGCTACG TTGAGGCGGC CTGGTACTCC TGGTGGGTGC GAGAGGGCTT CTTCAAACCG
    GAGTATCAGA CCAGGCTGCC CCAGGCCACA GGGGAGACGT TTTCCATGTG TATCCCACCT
    CCCAATGTCA CTGGCTCCCT GCATATCGGT CATGCACTGA CAGTAGCCAT ACAGGATGCC
    CTGGTGCGCT GGCACCGGAT GCGTGGGGAT CAGGTGCTAT GGATCCCTGG CTCAGATCAT
    GCAGGAATTG CTACACAAGC TGTGGTGGAG AAGCAACTGT GGAAGGAGCG AGGAATGAGG
    AGACATGAAC TGAGCCGGGA AAAATTCCTT AGGGAGGTGT GGAAGTGGAA GGACGAGAAA
    GGTGGAGAGA TCAGTGAGCA GCTCCAAGCT CTGGGGGCCT CCCTGGACTG GGACCGAGAG
    TGCTTCACTA TGGATGCTGG CTTCTCAGTG GCCGTGACCG AAGCTTTTGT GCGACTCTAC
    GAGGCAGGAT TGTTGTACCG GAGCCAGCAG CTTGTCAACT GGTCCTGCAC TTTAAGATCA
    GCCATCTCGG ATATTGAGGT GGAGAGCCGG CCCCTGCCTG GCCACACGGA GCTTCAACTG
    CCTGGCTGCC CCACTCCTGT GTCTTTTGGC CTCCTTGTGT CTGTGGCCTT CCCTGTGGAT
    GGAGAGCCTG ATGCAGAGGT CGTGGTAGGA ACTACAAGGC CAGAGACGTT GCCTGGAGAC
    GTTGCTGTGG CTGTTCATCC CGACGACTCC CGATACACAC ACCTGCATGG GCGACAGCTT
    CGTCACCCCT TGACTGGGCA GCTTCTCCCC CTCATCACAG ACTCCACTGT TCAGCCACAT
    GTGGGCACAG GGGCAGTGAA GGTGACTCCA GCGCATAGCC CTGCTGATGC TGAGCTGGGG
    GCCCGACACG GCTTGAGCCC TCTGAGTGTC ATCGCGGAGG ATGGCACCAT GACCTCTCTC
    TGTGGGGACT GGCTACAGGG TCTTCACCGA TTTGTGGCCC GGGAAAAGAT TATATCTGCG
    CTGCGGGAGC GGGGCCTGTT CCGGGGCCTC CAGAACCACC CCATGGTGCT TCCTATTTGC
    AGCCGTTCTG GGGACGTGAT AGAATATCTA CTGAAGAGCC AGTGGTTTGT CCGCTGCCGG
    GAGATGGGGG ACCGAGCTGC CCAGATGCAA GTGAGGTGCA GGAAGGGAAG CTCTAACGGC
    CTTTCTGTTC AGGCCGTGGA GTCAGGGGCT CTGGAGCTCA GTCCATCCTT CCACCAGAAG
    AACTGGCAGC ACTGGTTTTC CCACATCGGG GACTGGTGTG TCTCCCGGCA GCTGTGGTGG
    GGCCATCGGA TTCCCGCCTA CCTGGTTGTA GAGGAGCACA TGAAGGGTGA TGGAGAAGAC
    TGTTGGGTGG TTGGGCGGAC AGAGACCGAG GCCAGAAAAA TAGCTGCAGA ACTGACTGGG
    AGATCAGAGG CAGAGCTGAC CCTGCAGAGG GATCCTGATG TCCTGGACAC ATGGTTCTCT
    TCGGCTCTTT TCCCCTTTGC TGCCTTGGGC TGGCCCCAAG AGACCCCAGA CCTTGCTCGT
    TTCTACCCCC TGTCACTTTT GGAAACAGGC AGTGATCTCC TGTTGTTCTG GGTGGGCCGC
    ATGGTCATGT TGGGGACACA GCTCACAGGG CAGCTCCCCT TCACCAAGGT GCTTCTTCAT
    TCCATGGTTC GGGACGGGCA GGGCCGGAAG ATGAGCAAGT CCCTGGGGAA TGTATTGGAC
    CCACGAGACA TCATCAGTGG GGTGGAACTG CAGGTGCTGC AGGAAAAGCT GAAGGACGGG
    AACTTGGACT CCACAGAGCT GGCGATTGCG GCTGCGGCAC AGCTGTGTAG GCATCTGCCA
    CCCTTCTTAT CCCTCTGGTT GCAGAGGAAG GACTTCCCTC ATGGGATCCC TGAGTGTGGG
    ACAGATGCCC TGCGATTTGC CCTGTGCTCC CACAGGGCCT TGGGGGGCGA TGTACACTTG
    TCCGTCTCTG AGGTCTTGAG TTCCCGGCAT TTCTGCAACA AAATCTGGAA TGCCCTGCGT
    TTTATCCTCA ATGCCTTAGG AGAGGGATTT GTACCGCAGC CTGCAGAGGA GCTATCCCCC
    TCATCCCCCA CGGACGCCTG GATCCTGAGC CGTCTGGCCC ACACTGCCCA GGAGTGTGAG
    CGAGCCTTCC TTGCTCGGGA GCTGCCACTC ATCACCCATG CCCTCCACCA CTTCTGGCTC
    CACAGCCTCT GTGATGTCTA CTTGCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe292300
    Clone ID Related Accession (Same CDS sequence) XM_022425817.1
    Accession Version XM_022425817.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3039bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product valine--tRNA ligase, mitochondrial isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006594.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCTGCGGG TCTGGAGCGC CTCTCCGCCT TTGCAGACAC TCCTGATGCC TCGTTCGCCC 
    TTGGCCTCGC TTCCATCACG GTGCTGGGGG CTCGGGCCCT CTCAGGCCCT CCGCAGGTCC
    CACCCTCTCT CTACCCAGTC AGAGCCGTGC CGGTCCTCCC TCTCCCGGAG GAACCATGAA
    GCCAAACAGA AGCGCCAGCG GGAGAAGCAG ATGGCGCTGG AGGCTGGGAT CACCCCGAAG
    GGCAAGGCAC CTGCAGGATC CAGTAAGGCC TGGACTCCTA AGGAGGTAGT GTTGTATGAA
    GTCCCCACGG AACCGGGTGA AAAGAAAGAT GTGTACCGCC TCCTGCCTCC TGCATACAGT
    CCTCGCTACG TTGAGGCGGC CTGGTACTCC TGGTGGGTGC GAGAGGGCTT CTTCAAACCG
    GAGTATCAGA CCAGGCTGCC CCAGGCCACA GGGGAGACGT TTTCCATGTG TATCCCACCT
    CCCAATGTCA CTGGCTCCCT GCATATCGGT CATGCACTGA CAGTAGCCAT ACAGGATGCC
    CTGGTGCGCT GGCACCGGAT GCGTGGGGAT CAGGTGCTAT GGATCCCTGG CTCAGATCAT
    GCAGGAATTG CTACACAAGC TGTGGTGGAG AAGCAACTGT GGAAGGAGCG AGGAATGAGG
    AGACATGAAC TGAGCCGGGA AAAATTCCTT AGGGAGGTGT GGAAGTGGAA GGACGAGAAA
    GGTGGAGAGA TCAGTGAGCA GCTCCAAGCT CTGGGGGCCT CCCTGGACTG GGACCGAGAG
    TGCTTCACTA TGGATGCTGG CTTCTCAGTG GCCGTGACCG AAGCTTTTGT GCGACTCTAC
    GAGGCAGGAT TGTTGTACCG GAGCCAGCAG CTTGTCAACT GGTCCTGCAC TTTAAGATCA
    GCCATCTCGG ATATTGAGGT GGAGAGCCGG CCCCTGCCTG GCCACACGGA GCTTCAACTG
    CCTGGCTGCC CCACTCCTGT GTCTTTTGGC CTCCTTGTGT CTGTGGCCTT CCCTGTGGAT
    GGAGAGCCTG ATGCAGAGGT CGTGGTAGGA ACTACAAGGC CAGAGACGTT GCCTGGAGAC
    GTTGCTGTGG CTGTTCATCC CGACGACTCC CGATACACAC ACCTGCATGG GCGACAGCTT
    CGTCACCCCT TGACTGGGCA GCTTCTCCCC CTCATCACAG ACTCCACTGT TCAGCCACAT
    GTGGGCACAG GGGCAGTGAA GGTGACTCCA GCGCATAGCC CTGCTGATGC TGAGCTGGGG
    GCCCGACACG GCTTGAGCCC TCTGAGTGTC ATCGCGGAGG ATGGCACCAT GACCTCTCTC
    TGTGGGGACT GGCTACAGGG TCTTCACCGA TTTGTGGCCC GGGAAAAGAT TATATCTGCG
    CTGCGGGAGC GGGGCCTGTT CCGGGGCCTC CAGAACCACC CCATGGTGCT TCCTATTTGC
    AGCCGTTCTG GGGACGTGAT AGAATATCTA CTGAAGAGCC AGTGGTTTGT CCGCTGCCGG
    GAGATGGGGG ACCGAGCTGC CCAGATGCAA GTGAGGTGCA GGAAGGGAAG CTCTAACGGC
    CTTTCTGTTC AGGCCGTGGA GTCAGGGGCT CTGGAGCTCA GTCCATCCTT CCACCAGAAG
    AACTGGCAGC ACTGGTTTTC CCACATCGGG GACTGGTGTG TCTCCCGGCA GCTGTGGTGG
    GGCCATCGGA TTCCCGCCTA CCTGGTTGTA GAGGAGCACA TGAAGGGTGA TGGAGAAGAC
    TGTTGGGTGG TTGGGCGGAC AGAGACCGAG GCCAGAAAAA TAGCTGCAGA ACTGACTGGG
    AGATCAGAGG CAGAGCTGAC CCTGCAGAGG GATCCTGATG TCCTGGACAC ATGGTTCTCT
    TCGGCTCTTT TCCCCTTTGC TGCCTTGGGC TGGCCCCAAG AGACCCCAGA CCTTGCTCGT
    TTCTACCCCC TGTCACTTTT GGAAACAGGC AGTGATCTCC TGTTGTTCTG GGTGGGCCGC
    ATGGTCATGT TGGGGACACA GCTCACAGGG CAGCTCCCCT TCACCAAGGT GCTTCTTCAT
    TCCATGGTTC GGGACGGGCA GGGCCGGAAG ATGAGCAAGT CCCTGGGGAA TGTATTGGAC
    CCACGAGACA TCATCAGTGG GGTGGAACTG CAGGTGCTGC AGGAAAAGCT GAAGGACGGG
    AACTTGGACT CCACAGAGCT GGCGATTGCG GCTGCGGCAC AGCTGTGTAG GCATCTGCCA
    CCCTTCTTAT CCCTCTGGTT GCAGAGGAAG GACTTCCCTC ATGGGATCCC TGAGTGTGGG
    ACAGATGCCC TGCGATTTGC CCTGTGCTCC CACAGGGCCT TGGGGGGCGA TGTACACTTG
    TCCGTCTCTG AGGTCTTGAG TTCCCGGCAT TTCTGCAACA AAATCTGGAA TGCCCTGCGT
    TTTATCCTCA ATGCCTTAGG AGAGGGATTT GTACCGCAGC CTGCAGAGGA GCTATCCCCC
    TCATCCCCCA CGGACGCCTG GATCCTGAGC CGTCTGGCCC ACACTGCCCA GGAGTGTGAG
    CGAGCCTTCC TTGCTCGGGA GCTGCCACTC ATCACCCATG CCCTCCACCA CTTCTGGCTC
    CACAGCCTCT GTGATGTCTA CTTGGAAGCT GTGAAGCCAG TGCTTTCGCA CTCGCCCCAT
    CCCCAAGGCC CGCCTCAGGT CCTGTTCTCC TGCGCTGATG TGGGTCTCCG CCTCCTCGCC
    CCACTGATGC CCTTCCTGGC TGAAGAGCTC TGGCAGAGGC TGCCACCCAG GCCTGGCAGC
    AACTCTGCCC CTAGCATCTC TGTTGCTCCC TACCCCACCA CCCATAGCTT GGAGCACTGG
    CGCCAGCCTG CGCTGGAACA ACACTTCTCT CAGGTCCAGG AGGCTGTGCA GGTACTGAGG
    GCCCTCCGAG CCACGTACCA GCTCACCAAG GCCCGGCCCC GAGGGCCTGG TGGACCCCCA
    GGCCCATCTG GCTCTGCTAG CTGCCCGAAG ACGCAAGTTG CAGAAGCAGC TGGATGGCCT
    CATGGCCTGG ACCCCATCAG GGGGAGAGGA AGAGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022281525.1
    CDS105..3143
    Translation

    Target ORF information:

    RefSeq Version XM_022425817.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022425817.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCTGCGGG TCTGGAGCGC CTCTCCGCCT TTGCAGACAC TCCTGATGCC TCGTTCGCCC 
    TTGGCCTCGC TTCCATCACG GTGCTGGGGG CTCGGGCCCT CTCAGGCCCT CCGCAGGTCC
    CACCCTCTCT CTACCCAGTC AGAGCCGTGC CGGTCCTCCC TCTCCCGGAG GAACCATGAA
    GCCAAACAGA AGCGCCAGCG GGAGAAGCAG ATGGCGCTGG AGGCTGGGAT CACCCCGAAG
    GGCAAGGCAC CTGCAGGATC CAGTAAGGCC TGGACTCCTA AGGAGGTAGT GTTGTATGAA
    GTCCCCACGG AACCGGGTGA AAAGAAAGAT GTGTACCGCC TCCTGCCTCC TGCATACAGT
    CCTCGCTACG TTGAGGCGGC CTGGTACTCC TGGTGGGTGC GAGAGGGCTT CTTCAAACCG
    GAGTATCAGA CCAGGCTGCC CCAGGCCACA GGGGAGACGT TTTCCATGTG TATCCCACCT
    CCCAATGTCA CTGGCTCCCT GCATATCGGT CATGCACTGA CAGTAGCCAT ACAGGATGCC
    CTGGTGCGCT GGCACCGGAT GCGTGGGGAT CAGGTGCTAT GGATCCCTGG CTCAGATCAT
    GCAGGAATTG CTACACAAGC TGTGGTGGAG AAGCAACTGT GGAAGGAGCG AGGAATGAGG
    AGACATGAAC TGAGCCGGGA AAAATTCCTT AGGGAGGTGT GGAAGTGGAA GGACGAGAAA
    GGTGGAGAGA TCAGTGAGCA GCTCCAAGCT CTGGGGGCCT CCCTGGACTG GGACCGAGAG
    TGCTTCACTA TGGATGCTGG CTTCTCAGTG GCCGTGACCG AAGCTTTTGT GCGACTCTAC
    GAGGCAGGAT TGTTGTACCG GAGCCAGCAG CTTGTCAACT GGTCCTGCAC TTTAAGATCA
    GCCATCTCGG ATATTGAGGT GGAGAGCCGG CCCCTGCCTG GCCACACGGA GCTTCAACTG
    CCTGGCTGCC CCACTCCTGT GTCTTTTGGC CTCCTTGTGT CTGTGGCCTT CCCTGTGGAT
    GGAGAGCCTG ATGCAGAGGT CGTGGTAGGA ACTACAAGGC CAGAGACGTT GCCTGGAGAC
    GTTGCTGTGG CTGTTCATCC CGACGACTCC CGATACACAC ACCTGCATGG GCGACAGCTT
    CGTCACCCCT TGACTGGGCA GCTTCTCCCC CTCATCACAG ACTCCACTGT TCAGCCACAT
    GTGGGCACAG GGGCAGTGAA GGTGACTCCA GCGCATAGCC CTGCTGATGC TGAGCTGGGG
    GCCCGACACG GCTTGAGCCC TCTGAGTGTC ATCGCGGAGG ATGGCACCAT GACCTCTCTC
    TGTGGGGACT GGCTACAGGG TCTTCACCGA TTTGTGGCCC GGGAAAAGAT TATATCTGCG
    CTGCGGGAGC GGGGCCTGTT CCGGGGCCTC CAGAACCACC CCATGGTGCT TCCTATTTGC
    AGCCGTTCTG GGGACGTGAT AGAATATCTA CTGAAGAGCC AGTGGTTTGT CCGCTGCCGG
    GAGATGGGGG ACCGAGCTGC CCAGATGCAA GTGAGGTGCA GGAAGGGAAG CTCTAACGGC
    CTTTCTGTTC AGGCCGTGGA GTCAGGGGCT CTGGAGCTCA GTCCATCCTT CCACCAGAAG
    AACTGGCAGC ACTGGTTTTC CCACATCGGG GACTGGTGTG TCTCCCGGCA GCTGTGGTGG
    GGCCATCGGA TTCCCGCCTA CCTGGTTGTA GAGGAGCACA TGAAGGGTGA TGGAGAAGAC
    TGTTGGGTGG TTGGGCGGAC AGAGACCGAG GCCAGAAAAA TAGCTGCAGA ACTGACTGGG
    AGATCAGAGG CAGAGCTGAC CCTGCAGAGG GATCCTGATG TCCTGGACAC ATGGTTCTCT
    TCGGCTCTTT TCCCCTTTGC TGCCTTGGGC TGGCCCCAAG AGACCCCAGA CCTTGCTCGT
    TTCTACCCCC TGTCACTTTT GGAAACAGGC AGTGATCTCC TGTTGTTCTG GGTGGGCCGC
    ATGGTCATGT TGGGGACACA GCTCACAGGG CAGCTCCCCT TCACCAAGGT GCTTCTTCAT
    TCCATGGTTC GGGACGGGCA GGGCCGGAAG ATGAGCAAGT CCCTGGGGAA TGTATTGGAC
    CCACGAGACA TCATCAGTGG GGTGGAACTG CAGGTGCTGC AGGAAAAGCT GAAGGACGGG
    AACTTGGACT CCACAGAGCT GGCGATTGCG GCTGCGGCAC AGCTGTGTAG GCATCTGCCA
    CCCTTCTTAT CCCTCTGGTT GCAGAGGAAG GACTTCCCTC ATGGGATCCC TGAGTGTGGG
    ACAGATGCCC TGCGATTTGC CCTGTGCTCC CACAGGGCCT TGGGGGGCGA TGTACACTTG
    TCCGTCTCTG AGGTCTTGAG TTCCCGGCAT TTCTGCAACA AAATCTGGAA TGCCCTGCGT
    TTTATCCTCA ATGCCTTAGG AGAGGGATTT GTACCGCAGC CTGCAGAGGA GCTATCCCCC
    TCATCCCCCA CGGACGCCTG GATCCTGAGC CGTCTGGCCC ACACTGCCCA GGAGTGTGAG
    CGAGCCTTCC TTGCTCGGGA GCTGCCACTC ATCACCCATG CCCTCCACCA CTTCTGGCTC
    CACAGCCTCT GTGATGTCTA CTTGGAAGCT GTGAAGCCAG TGCTTTCGCA CTCGCCCCAT
    CCCCAAGGCC CGCCTCAGGT CCTGTTCTCC TGCGCTGATG TGGGTCTCCG CCTCCTCGCC
    CCACTGATGC CCTTCCTGGC TGAAGAGCTC TGGCAGAGGC TGCCACCCAG GCCTGGCAGC
    AACTCTGCCC CTAGCATCTC TGTTGCTCCC TACCCCACCA CCCATAGCTT GGAGCACTGG
    CGCCAGCCTG CGCTGGAACA ACACTTCTCT CAGGTCCAGG AGGCTGTGCA GGTACTGAGG
    GCCCTCCGAG CCACGTACCA GCTCACCAAG GCCCGGCCCC GAGGGCCTGG TGGACCCCCA
    GGCCCATCTG GCTCTGCTAG CTGCCCGAAG ACGCAAGTTG CAGAAGCAGC TGGATGGCCT
    CATGGCCTGG ACCCCATCAG GGGGAGAGGA AGAGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe39497
    Clone ID Related Accession (Same CDS sequence) XM_014118299.1 , XM_014118299.2
    Accession Version XM_014118299.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3324bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product valine--tRNA ligase, mitochondrial isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726081.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCTGCGGG TCTGGAGCGC CTCTCCGCCT TTGCAGACAC TCCTGATGCC TCGTTCGCCC 
    TTGGCCTCGC TTCCATCACG GTGCTGGGGG CTCGGGCCCT CTCAGGCCCT CCGCAGGTCC
    CACCCTCTCT CTACCCAGTC AGAGCCGTGC CGGTCCTCCC TCTCCCGGAG GAACCATGAA
    GCCAAACAGA AGCGCCAGCG GGAGAAGCAG ATGGCGCTGG AGGCTGGGAT CACCCCGAAG
    GGCAAGGCAC CTGCAGGATC CAGTAAGGCC TGGACTCCTA AGGAGGTAGT GTTGTATGAA
    GTCCCCACGG AACCGGGTGA AAAGAAAGAT GTGTACCGCC TCCTGCCTCC TGCATACAGT
    CCTCGCTACG TTGAGGCGGC CTGGTACTCC TGGTGGGTGC GAGAGGGCTT CTTCAAACCG
    GAGTATCAGA CCAGGCTGCC CCAGGCCACA GGGGAGACGT TTTCCATGTG TATCCCACCT
    CCCAATGTCA CTGGCTCCCT GCATATCGGT CATGCACTGA CAGTAGCCAT ACAGGATGCC
    CTGGTGCGCT GGCACCGGAT GCGTGGGGAT CAGGTGCTAT GGATCCCTGG CTCAGATCAT
    GCAGGAATTG CTACACAAGC TGTGGTGGAG AAGCAACTGT GGAAGGAGCG AGGAATGAGG
    AGACATGAAC TGAGCCGGGA AAAATTCCTT AGGGAGGTGT GGAAGTGGAA GGACGAGAAA
    GGTGGAGAGA TCAGTGAGCA GCTCCAAGCT CTGGGGGCCT CCCTGGACTG GGACCGAGAG
    TGCTTCACTA TGGATGCTGG CTTCTCAGTG GCCGTGACCG AAGCTTTTGT GCGACTCTAC
    GAGGCAGGAT TGTTGTACCG GAGCCAGCAG CTTGTCAACT GGTCCTGCAC TTTAAGATCA
    GCCATCTCGG ATATTGAGGT GGAGAGCCGG CCCCTGCCTG GCCACACGGA GCTTCAACTG
    CCTGGCTGCC CCACTCCTGT GTCTTTTGGC CTCCTTGTGT CTGTGGCCTT CCCTGTGGAT
    GGAGAGCCTG ATGCAGAGGT CGTGGTAGGA ACTACAAGGC CAGAGACGTT GCCTGGAGAC
    GTTGCTGTGG CTGTTCATCC CGACGACTCC CGATACACAC ACCTGCATGG GCGACAGCTT
    CGTCACCCCT TGACTGGGCA GCTTCTCCCC CTCATCACAG ACTCCACTGT TCAGCCACAT
    GTGGGCACAG GGGCAGTGAA GGTGACTCCA GCGCATAGCC CTGCTGATGC TGAGCTGGGG
    GCCCGACACG GCTTGAGCCC TCTGAGTGTC ATCGCGGAGG ATGGCACCAT GACCTCTCTC
    TGTGGGGACT GGCTACAGGG TCTTCACCGA TTTGTGGCCC GGGAAAAGAT TATATCTGCG
    CTGCGGGAGC GGGGCCTGTT CCGGGGCCTC CAGAACCACC CCATGGTGCT TCCTATTTGC
    AGCCGTTCTG GGGACGTGAT AGAATATCTA CTGAAGAGCC AGTGGTTTGT CCGCTGCCGG
    GAGATGGGGG ACCGAGCTGC CCAGATGCAA GTGAGGTGCA GGAAGGGAAG CTCTAACGGC
    CTTTCTGTTC AGGCCGTGGA GTCAGGGGCT CTGGAGCTCA GTCCATCCTT CCACCAGAAG
    AACTGGCAGC ACTGGTTTTC CCACATCGGG GACTGGTGTG TCTCCCGGCA GCTGTGGTGG
    GGCCATCGGA TTCCCGCCTA CCTGGTTGTA GAGGAGCACA TGAAGGGTGA TGGAGAAGAC
    TGTTGGGTGG TTGGGCGGAC AGAGACCGAG GCCAGAAAAA TAGCTGCAGA ACTGACTGGG
    AGATCAGAGG CAGAGCTGAC CCTGCAGAGG GATCCTGATG TCCTGGACAC ATGGTTCTCT
    TCGGCTCTTT TCCCCTTTGC TGCCTTGGGC TGGCCCCAAG AGACCCCAGA CCTTGCTCGT
    TTCTACCCCC TGTCACTTTT GGAAACAGGC AGTGATCTCC TGTTGTTCTG GGTGGGCCGC
    ATGGTCATGT TGGGGACACA GCTCACAGGG CAGCTCCCCT TCACCAAGGT GCTTCTTCAT
    TCCATGGTTC GGGACGGGCA GGGCCGGAAG ATGAGCAAGT CCCTGGGGAA TGTATTGGAC
    CCACGAGACA TCATCAGTGG GGTGGAACTG CAGGTGCTGC AGGAAAAGCT GAAGGACGGG
    AACTTGGACT CCACAGAGCT GGCGATTGCG GCTGCGGCAC AGCTGTGTAG GCATCTGCCA
    CCCTTCTTAT CCCTCTGGTT GCAGAGGAAG GACTTCCCTC ATGGGATCCC TGAGTGTGGG
    ACAGATGCCC TGCGATTTGC CCTGTGCTCC CACAGGGCCT TGGGGGGCGA TGTACACTTG
    TCCGTCTCTG AGGTCTTGAG TTCCCGGCAT TTCTGCAACA AAATCTGGAA TGCCCTGCGT
    TTTATCCTCA ATGCCTTAGG AGAGGGATTT GTACCGCAGC CTGCAGAGGA GCTATCCCCC
    TCATCCCCCA CGGACGCCTG GATCCTGAGC CGTCTGGCCC ACACTGCCCA GGAGTGTGAG
    CGAGCCTTCC TTGCTCGGGA GCTGCCACTC ATCACCCATG CCCTCCACCA CTTCTGGCTC
    CACAGCCTCT GTGATGTCTA CTTGGAAGCT GTGAAGCCAG TGCTTTCGCA CTCGCCCCAT
    CCCCAAGGCC CGCCTCAGGT CCTGTTCTCC TGCGCTGATG TGGGTCTCCG CCTCCTCGCC
    CCACTGATGC CCTTCCTGGC TGAAGAGCTC TGGCAGAGGC TGCCACCCAG GCCTGGCAGC
    AACTCTGCCC CTAGCATCTC TGTTGCTCCC TACCCCACCA CCCATAGCTT GGAGCACTGG
    CGCCAGCCTG CGCTGGAACA ACACTTCTCT CAGGTCCAGG AGGCTGTGCA GGTACTGAGG
    GCCCTCCGAG CCACGTACCA GCTCACCAAG GCCCGGCCCC GAGTACTGCT GCAGAGCTCA
    GAGCCTGGAG AGCTGAGCCT CTTCAAGCCC TTCCTGGAGC CCTTGGCCAC ACTGGGCCAC
    TGTGGGGCCG TGGGCCTGTT ACCCCCAGGT GTAGCGGCAC CCTCCGGCTG GGCTCAGGCC
    CCACTCAGTG ACACCATTCA GGTGTACATG GAGCTACAGG GCCTGGTGGA CCCCCAGGCC
    CATCTGGCTC TGCTAGCTGC CCGAAGACGC AAGTTGCAGA AGCAGCTGGA TGGCCTCATG
    GCCTGGACCC CATCAGGGGG AGAGGAAGAG ACTAAGAGGC AGCAGAGGCT TTCTTCCCTC
    CAGCTGGAGT TGTCAAAGCT GGACAAGGCA GCCTCTCACC TCCGGCAGCT GACGGATGCA
    TGTCCCAGCC CCAAGGAGCT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013973774.1
    CDS131..3454
    Translation

    Target ORF information:

    RefSeq Version XM_014118299.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014118299.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCTGCGGG TCTGGAGCGC CTCTCCGCCT TTGCAGACAC TCCTGATGCC TCGTTCGCCC 
    TTGGCCTCGC TTCCATCACG GTGCTGGGGG CTCGGGCCCT CTCAGGCCCT CCGCAGGTCC
    CACCCTCTCT CTACCCAGTC AGAGCCGTGC CGGTCCTCCC TCTCCCGGAG GAACCATGAA
    GCCAAACAGA AGCGCCAGCG GGAGAAGCAG ATGGCGCTGG AGGCTGGGAT CACCCCGAAG
    GGCAAGGCAC CTGCAGGATC CAGTAAGGCC TGGACTCCTA AGGAGGTAGT GTTGTATGAA
    GTCCCCACGG AACCGGGTGA AAAGAAAGAT GTGTACCGCC TCCTGCCTCC TGCATACAGT
    CCTCGCTACG TTGAGGCGGC CTGGTACTCC TGGTGGGTGC GAGAGGGCTT CTTCAAACCG
    GAGTATCAGA CCAGGCTGCC CCAGGCCACA GGGGAGACGT TTTCCATGTG TATCCCACCT
    CCCAATGTCA CTGGCTCCCT GCATATCGGT CATGCACTGA CAGTAGCCAT ACAGGATGCC
    CTGGTGCGCT GGCACCGGAT GCGTGGGGAT CAGGTGCTAT GGATCCCTGG CTCAGATCAT
    GCAGGAATTG CTACACAAGC TGTGGTGGAG AAGCAACTGT GGAAGGAGCG AGGAATGAGG
    AGACATGAAC TGAGCCGGGA AAAATTCCTT AGGGAGGTGT GGAAGTGGAA GGACGAGAAA
    GGTGGAGAGA TCAGTGAGCA GCTCCAAGCT CTGGGGGCCT CCCTGGACTG GGACCGAGAG
    TGCTTCACTA TGGATGCTGG CTTCTCAGTG GCCGTGACCG AAGCTTTTGT GCGACTCTAC
    GAGGCAGGAT TGTTGTACCG GAGCCAGCAG CTTGTCAACT GGTCCTGCAC TTTAAGATCA
    GCCATCTCGG ATATTGAGGT GGAGAGCCGG CCCCTGCCTG GCCACACGGA GCTTCAACTG
    CCTGGCTGCC CCACTCCTGT GTCTTTTGGC CTCCTTGTGT CTGTGGCCTT CCCTGTGGAT
    GGAGAGCCTG ATGCAGAGGT CGTGGTAGGA ACTACAAGGC CAGAGACGTT GCCTGGAGAC
    GTTGCTGTGG CTGTTCATCC CGACGACTCC CGATACACAC ACCTGCATGG GCGACAGCTT
    CGTCACCCCT TGACTGGGCA GCTTCTCCCC CTCATCACAG ACTCCACTGT TCAGCCACAT
    GTGGGCACAG GGGCAGTGAA GGTGACTCCA GCGCATAGCC CTGCTGATGC TGAGCTGGGG
    GCCCGACACG GCTTGAGCCC TCTGAGTGTC ATCGCGGAGG ATGGCACCAT GACCTCTCTC
    TGTGGGGACT GGCTACAGGG TCTTCACCGA TTTGTGGCCC GGGAAAAGAT TATATCTGCG
    CTGCGGGAGC GGGGCCTGTT CCGGGGCCTC CAGAACCACC CCATGGTGCT TCCTATTTGC
    AGCCGTTCTG GGGACGTGAT AGAATATCTA CTGAAGAGCC AGTGGTTTGT CCGCTGCCGG
    GAGATGGGGG ACCGAGCTGC CCAGATGCAA GTGAGGTGCA GGAAGGGAAG CTCTAACGGC
    CTTTCTGTTC AGGCCGTGGA GTCAGGGGCT CTGGAGCTCA GTCCATCCTT CCACCAGAAG
    AACTGGCAGC ACTGGTTTTC CCACATCGGG GACTGGTGTG TCTCCCGGCA GCTGTGGTGG
    GGCCATCGGA TTCCCGCCTA CCTGGTTGTA GAGGAGCACA TGAAGGGTGA TGGAGAAGAC
    TGTTGGGTGG TTGGGCGGAC AGAGACCGAG GCCAGAAAAA TAGCTGCAGA ACTGACTGGG
    AGATCAGAGG CAGAGCTGAC CCTGCAGAGG GATCCTGATG TCCTGGACAC ATGGTTCTCT
    TCGGCTCTTT TCCCCTTTGC TGCCTTGGGC TGGCCCCAAG AGACCCCAGA CCTTGCTCGT
    TTCTACCCCC TGTCACTTTT GGAAACAGGC AGTGATCTCC TGTTGTTCTG GGTGGGCCGC
    ATGGTCATGT TGGGGACACA GCTCACAGGG CAGCTCCCCT TCACCAAGGT GCTTCTTCAT
    TCCATGGTTC GGGACGGGCA GGGCCGGAAG ATGAGCAAGT CCCTGGGGAA TGTATTGGAC
    CCACGAGACA TCATCAGTGG GGTGGAACTG CAGGTGCTGC AGGAAAAGCT GAAGGACGGG
    AACTTGGACT CCACAGAGCT GGCGATTGCG GCTGCGGCAC AGCTGTGTAG GCATCTGCCA
    CCCTTCTTAT CCCTCTGGTT GCAGAGGAAG GACTTCCCTC ATGGGATCCC TGAGTGTGGG
    ACAGATGCCC TGCGATTTGC CCTGTGCTCC CACAGGGCCT TGGGGGGCGA TGTACACTTG
    TCCGTCTCTG AGGTCTTGAG TTCCCGGCAT TTCTGCAACA AAATCTGGAA TGCCCTGCGT
    TTTATCCTCA ATGCCTTAGG AGAGGGATTT GTACCGCAGC CTGCAGAGGA GCTATCCCCC
    TCATCCCCCA CGGACGCCTG GATCCTGAGC CGTCTGGCCC ACACTGCCCA GGAGTGTGAG
    CGAGCCTTCC TTGCTCGGGA GCTGCCACTC ATCACCCATG CCCTCCACCA CTTCTGGCTC
    CACAGCCTCT GTGATGTCTA CTTGGAAGCT GTGAAGCCAG TGCTTTCGCA CTCGCCCCAT
    CCCCAAGGCC CGCCTCAGGT CCTGTTCTCC TGCGCTGATG TGGGTCTCCG CCTCCTCGCC
    CCACTGATGC CCTTCCTGGC TGAAGAGCTC TGGCAGAGGC TGCCACCCAG GCCTGGCAGC
    AACTCTGCCC CTAGCATCTC TGTTGCTCCC TACCCCACCA CCCATAGCTT GGAGCACTGG
    CGCCAGCCTG CGCTGGAACA ACACTTCTCT CAGGTCCAGG AGGCTGTGCA GGTACTGAGG
    GCCCTCCGAG CCACGTACCA GCTCACCAAG GCCCGGCCCC GAGTACTGCT GCAGAGCTCA
    GAGCCTGGAG AGCTGAGCCT CTTCAAGCCC TTCCTGGAGC CCTTGGCCAC ACTGGGCCAC
    TGTGGGGCCG TGGGCCTGTT ACCCCCAGGT GTAGCGGCAC CCTCCGGCTG GGCTCAGGCC
    CCACTCAGTG ACACCATTCA GGTGTACATG GAGCTACAGG GCCTGGTGGA CCCCCAGGCC
    CATCTGGCTC TGCTAGCTGC CCGAAGACGC AAGTTGCAGA AGCAGCTGGA TGGCCTCATG
    GCCTGGACCC CATCAGGGGG AGAGGAAGAG ACTAAGAGGC AGCAGAGGCT TTCTTCCCTC
    CAGCTGGAGT TGTCAAAGCT GGACAAGGCA GCCTCTCACC TCCGGCAGCT GACGGATGCA
    TGTCCCAGCC CCAAGGAGCT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe39497
    Clone ID Related Accession (Same CDS sequence) XM_014118299.1 , XM_014118299.2
    Accession Version XM_014118299.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3324bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product valine--tRNA ligase, mitochondrial isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006594.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014118299.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCTGCGGG TCTGGAGCGC CTCTCCGCCT TTGCAGACAC TCCTGATGCC TCGTTCGCCC 
    TTGGCCTCGC TTCCATCACG GTGCTGGGGG CTCGGGCCCT CTCAGGCCCT CCGCAGGTCC
    CACCCTCTCT CTACCCAGTC AGAGCCGTGC CGGTCCTCCC TCTCCCGGAG GAACCATGAA
    GCCAAACAGA AGCGCCAGCG GGAGAAGCAG ATGGCGCTGG AGGCTGGGAT CACCCCGAAG
    GGCAAGGCAC CTGCAGGATC CAGTAAGGCC TGGACTCCTA AGGAGGTAGT GTTGTATGAA
    GTCCCCACGG AACCGGGTGA AAAGAAAGAT GTGTACCGCC TCCTGCCTCC TGCATACAGT
    CCTCGCTACG TTGAGGCGGC CTGGTACTCC TGGTGGGTGC GAGAGGGCTT CTTCAAACCG
    GAGTATCAGA CCAGGCTGCC CCAGGCCACA GGGGAGACGT TTTCCATGTG TATCCCACCT
    CCCAATGTCA CTGGCTCCCT GCATATCGGT CATGCACTGA CAGTAGCCAT ACAGGATGCC
    CTGGTGCGCT GGCACCGGAT GCGTGGGGAT CAGGTGCTAT GGATCCCTGG CTCAGATCAT
    GCAGGAATTG CTACACAAGC TGTGGTGGAG AAGCAACTGT GGAAGGAGCG AGGAATGAGG
    AGACATGAAC TGAGCCGGGA AAAATTCCTT AGGGAGGTGT GGAAGTGGAA GGACGAGAAA
    GGTGGAGAGA TCAGTGAGCA GCTCCAAGCT CTGGGGGCCT CCCTGGACTG GGACCGAGAG
    TGCTTCACTA TGGATGCTGG CTTCTCAGTG GCCGTGACCG AAGCTTTTGT GCGACTCTAC
    GAGGCAGGAT TGTTGTACCG GAGCCAGCAG CTTGTCAACT GGTCCTGCAC TTTAAGATCA
    GCCATCTCGG ATATTGAGGT GGAGAGCCGG CCCCTGCCTG GCCACACGGA GCTTCAACTG
    CCTGGCTGCC CCACTCCTGT GTCTTTTGGC CTCCTTGTGT CTGTGGCCTT CCCTGTGGAT
    GGAGAGCCTG ATGCAGAGGT CGTGGTAGGA ACTACAAGGC CAGAGACGTT GCCTGGAGAC
    GTTGCTGTGG CTGTTCATCC CGACGACTCC CGATACACAC ACCTGCATGG GCGACAGCTT
    CGTCACCCCT TGACTGGGCA GCTTCTCCCC CTCATCACAG ACTCCACTGT TCAGCCACAT
    GTGGGCACAG GGGCAGTGAA GGTGACTCCA GCGCATAGCC CTGCTGATGC TGAGCTGGGG
    GCCCGACACG GCTTGAGCCC TCTGAGTGTC ATCGCGGAGG ATGGCACCAT GACCTCTCTC
    TGTGGGGACT GGCTACAGGG TCTTCACCGA TTTGTGGCCC GGGAAAAGAT TATATCTGCG
    CTGCGGGAGC GGGGCCTGTT CCGGGGCCTC CAGAACCACC CCATGGTGCT TCCTATTTGC
    AGCCGTTCTG GGGACGTGAT AGAATATCTA CTGAAGAGCC AGTGGTTTGT CCGCTGCCGG
    GAGATGGGGG ACCGAGCTGC CCAGATGCAA GTGAGGTGCA GGAAGGGAAG CTCTAACGGC
    CTTTCTGTTC AGGCCGTGGA GTCAGGGGCT CTGGAGCTCA GTCCATCCTT CCACCAGAAG
    AACTGGCAGC ACTGGTTTTC CCACATCGGG GACTGGTGTG TCTCCCGGCA GCTGTGGTGG
    GGCCATCGGA TTCCCGCCTA CCTGGTTGTA GAGGAGCACA TGAAGGGTGA TGGAGAAGAC
    TGTTGGGTGG TTGGGCGGAC AGAGACCGAG GCCAGAAAAA TAGCTGCAGA ACTGACTGGG
    AGATCAGAGG CAGAGCTGAC CCTGCAGAGG GATCCTGATG TCCTGGACAC ATGGTTCTCT
    TCGGCTCTTT TCCCCTTTGC TGCCTTGGGC TGGCCCCAAG AGACCCCAGA CCTTGCTCGT
    TTCTACCCCC TGTCACTTTT GGAAACAGGC AGTGATCTCC TGTTGTTCTG GGTGGGCCGC
    ATGGTCATGT TGGGGACACA GCTCACAGGG CAGCTCCCCT TCACCAAGGT GCTTCTTCAT
    TCCATGGTTC GGGACGGGCA GGGCCGGAAG ATGAGCAAGT CCCTGGGGAA TGTATTGGAC
    CCACGAGACA TCATCAGTGG GGTGGAACTG CAGGTGCTGC AGGAAAAGCT GAAGGACGGG
    AACTTGGACT CCACAGAGCT GGCGATTGCG GCTGCGGCAC AGCTGTGTAG GCATCTGCCA
    CCCTTCTTAT CCCTCTGGTT GCAGAGGAAG GACTTCCCTC ATGGGATCCC TGAGTGTGGG
    ACAGATGCCC TGCGATTTGC CCTGTGCTCC CACAGGGCCT TGGGGGGCGA TGTACACTTG
    TCCGTCTCTG AGGTCTTGAG TTCCCGGCAT TTCTGCAACA AAATCTGGAA TGCCCTGCGT
    TTTATCCTCA ATGCCTTAGG AGAGGGATTT GTACCGCAGC CTGCAGAGGA GCTATCCCCC
    TCATCCCCCA CGGACGCCTG GATCCTGAGC CGTCTGGCCC ACACTGCCCA GGAGTGTGAG
    CGAGCCTTCC TTGCTCGGGA GCTGCCACTC ATCACCCATG CCCTCCACCA CTTCTGGCTC
    CACAGCCTCT GTGATGTCTA CTTGGAAGCT GTGAAGCCAG TGCTTTCGCA CTCGCCCCAT
    CCCCAAGGCC CGCCTCAGGT CCTGTTCTCC TGCGCTGATG TGGGTCTCCG CCTCCTCGCC
    CCACTGATGC CCTTCCTGGC TGAAGAGCTC TGGCAGAGGC TGCCACCCAG GCCTGGCAGC
    AACTCTGCCC CTAGCATCTC TGTTGCTCCC TACCCCACCA CCCATAGCTT GGAGCACTGG
    CGCCAGCCTG CGCTGGAACA ACACTTCTCT CAGGTCCAGG AGGCTGTGCA GGTACTGAGG
    GCCCTCCGAG CCACGTACCA GCTCACCAAG GCCCGGCCCC GAGTACTGCT GCAGAGCTCA
    GAGCCTGGAG AGCTGAGCCT CTTCAAGCCC TTCCTGGAGC CCTTGGCCAC ACTGGGCCAC
    TGTGGGGCCG TGGGCCTGTT ACCCCCAGGT GTAGCGGCAC CCTCCGGCTG GGCTCAGGCC
    CCACTCAGTG ACACCATTCA GGTGTACATG GAGCTACAGG GCCTGGTGGA CCCCCAGGCC
    CATCTGGCTC TGCTAGCTGC CCGAAGACGC AAGTTGCAGA AGCAGCTGGA TGGCCTCATG
    GCCTGGACCC CATCAGGGGG AGAGGAAGAG ACTAAGAGGC AGCAGAGGCT TTCTTCCCTC
    CAGCTGGAGT TGTCAAAGCT GGACAAGGCA GCCTCTCACC TCCGGCAGCT GACGGATGCA
    TGTCCCAGCC CCAAGGAGCT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013973774.1
    CDS103..3426
    Translation

    Target ORF information:

    RefSeq Version XM_014118299.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014118299.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCTGCGGG TCTGGAGCGC CTCTCCGCCT TTGCAGACAC TCCTGATGCC TCGTTCGCCC 
    TTGGCCTCGC TTCCATCACG GTGCTGGGGG CTCGGGCCCT CTCAGGCCCT CCGCAGGTCC
    CACCCTCTCT CTACCCAGTC AGAGCCGTGC CGGTCCTCCC TCTCCCGGAG GAACCATGAA
    GCCAAACAGA AGCGCCAGCG GGAGAAGCAG ATGGCGCTGG AGGCTGGGAT CACCCCGAAG
    GGCAAGGCAC CTGCAGGATC CAGTAAGGCC TGGACTCCTA AGGAGGTAGT GTTGTATGAA
    GTCCCCACGG AACCGGGTGA AAAGAAAGAT GTGTACCGCC TCCTGCCTCC TGCATACAGT
    CCTCGCTACG TTGAGGCGGC CTGGTACTCC TGGTGGGTGC GAGAGGGCTT CTTCAAACCG
    GAGTATCAGA CCAGGCTGCC CCAGGCCACA GGGGAGACGT TTTCCATGTG TATCCCACCT
    CCCAATGTCA CTGGCTCCCT GCATATCGGT CATGCACTGA CAGTAGCCAT ACAGGATGCC
    CTGGTGCGCT GGCACCGGAT GCGTGGGGAT CAGGTGCTAT GGATCCCTGG CTCAGATCAT
    GCAGGAATTG CTACACAAGC TGTGGTGGAG AAGCAACTGT GGAAGGAGCG AGGAATGAGG
    AGACATGAAC TGAGCCGGGA AAAATTCCTT AGGGAGGTGT GGAAGTGGAA GGACGAGAAA
    GGTGGAGAGA TCAGTGAGCA GCTCCAAGCT CTGGGGGCCT CCCTGGACTG GGACCGAGAG
    TGCTTCACTA TGGATGCTGG CTTCTCAGTG GCCGTGACCG AAGCTTTTGT GCGACTCTAC
    GAGGCAGGAT TGTTGTACCG GAGCCAGCAG CTTGTCAACT GGTCCTGCAC TTTAAGATCA
    GCCATCTCGG ATATTGAGGT GGAGAGCCGG CCCCTGCCTG GCCACACGGA GCTTCAACTG
    CCTGGCTGCC CCACTCCTGT GTCTTTTGGC CTCCTTGTGT CTGTGGCCTT CCCTGTGGAT
    GGAGAGCCTG ATGCAGAGGT CGTGGTAGGA ACTACAAGGC CAGAGACGTT GCCTGGAGAC
    GTTGCTGTGG CTGTTCATCC CGACGACTCC CGATACACAC ACCTGCATGG GCGACAGCTT
    CGTCACCCCT TGACTGGGCA GCTTCTCCCC CTCATCACAG ACTCCACTGT TCAGCCACAT
    GTGGGCACAG GGGCAGTGAA GGTGACTCCA GCGCATAGCC CTGCTGATGC TGAGCTGGGG
    GCCCGACACG GCTTGAGCCC TCTGAGTGTC ATCGCGGAGG ATGGCACCAT GACCTCTCTC
    TGTGGGGACT GGCTACAGGG TCTTCACCGA TTTGTGGCCC GGGAAAAGAT TATATCTGCG
    CTGCGGGAGC GGGGCCTGTT CCGGGGCCTC CAGAACCACC CCATGGTGCT TCCTATTTGC
    AGCCGTTCTG GGGACGTGAT AGAATATCTA CTGAAGAGCC AGTGGTTTGT CCGCTGCCGG
    GAGATGGGGG ACCGAGCTGC CCAGATGCAA GTGAGGTGCA GGAAGGGAAG CTCTAACGGC
    CTTTCTGTTC AGGCCGTGGA GTCAGGGGCT CTGGAGCTCA GTCCATCCTT CCACCAGAAG
    AACTGGCAGC ACTGGTTTTC CCACATCGGG GACTGGTGTG TCTCCCGGCA GCTGTGGTGG
    GGCCATCGGA TTCCCGCCTA CCTGGTTGTA GAGGAGCACA TGAAGGGTGA TGGAGAAGAC
    TGTTGGGTGG TTGGGCGGAC AGAGACCGAG GCCAGAAAAA TAGCTGCAGA ACTGACTGGG
    AGATCAGAGG CAGAGCTGAC CCTGCAGAGG GATCCTGATG TCCTGGACAC ATGGTTCTCT
    TCGGCTCTTT TCCCCTTTGC TGCCTTGGGC TGGCCCCAAG AGACCCCAGA CCTTGCTCGT
    TTCTACCCCC TGTCACTTTT GGAAACAGGC AGTGATCTCC TGTTGTTCTG GGTGGGCCGC
    ATGGTCATGT TGGGGACACA GCTCACAGGG CAGCTCCCCT TCACCAAGGT GCTTCTTCAT
    TCCATGGTTC GGGACGGGCA GGGCCGGAAG ATGAGCAAGT CCCTGGGGAA TGTATTGGAC
    CCACGAGACA TCATCAGTGG GGTGGAACTG CAGGTGCTGC AGGAAAAGCT GAAGGACGGG
    AACTTGGACT CCACAGAGCT GGCGATTGCG GCTGCGGCAC AGCTGTGTAG GCATCTGCCA
    CCCTTCTTAT CCCTCTGGTT GCAGAGGAAG GACTTCCCTC ATGGGATCCC TGAGTGTGGG
    ACAGATGCCC TGCGATTTGC CCTGTGCTCC CACAGGGCCT TGGGGGGCGA TGTACACTTG
    TCCGTCTCTG AGGTCTTGAG TTCCCGGCAT TTCTGCAACA AAATCTGGAA TGCCCTGCGT
    TTTATCCTCA ATGCCTTAGG AGAGGGATTT GTACCGCAGC CTGCAGAGGA GCTATCCCCC
    TCATCCCCCA CGGACGCCTG GATCCTGAGC CGTCTGGCCC ACACTGCCCA GGAGTGTGAG
    CGAGCCTTCC TTGCTCGGGA GCTGCCACTC ATCACCCATG CCCTCCACCA CTTCTGGCTC
    CACAGCCTCT GTGATGTCTA CTTGGAAGCT GTGAAGCCAG TGCTTTCGCA CTCGCCCCAT
    CCCCAAGGCC CGCCTCAGGT CCTGTTCTCC TGCGCTGATG TGGGTCTCCG CCTCCTCGCC
    CCACTGATGC CCTTCCTGGC TGAAGAGCTC TGGCAGAGGC TGCCACCCAG GCCTGGCAGC
    AACTCTGCCC CTAGCATCTC TGTTGCTCCC TACCCCACCA CCCATAGCTT GGAGCACTGG
    CGCCAGCCTG CGCTGGAACA ACACTTCTCT CAGGTCCAGG AGGCTGTGCA GGTACTGAGG
    GCCCTCCGAG CCACGTACCA GCTCACCAAG GCCCGGCCCC GAGTACTGCT GCAGAGCTCA
    GAGCCTGGAG AGCTGAGCCT CTTCAAGCCC TTCCTGGAGC CCTTGGCCAC ACTGGGCCAC
    TGTGGGGCCG TGGGCCTGTT ACCCCCAGGT GTAGCGGCAC CCTCCGGCTG GGCTCAGGCC
    CCACTCAGTG ACACCATTCA GGTGTACATG GAGCTACAGG GCCTGGTGGA CCCCCAGGCC
    CATCTGGCTC TGCTAGCTGC CCGAAGACGC AAGTTGCAGA AGCAGCTGGA TGGCCTCATG
    GCCTGGACCC CATCAGGGGG AGAGGAAGAG ACTAAGAGGC AGCAGAGGCT TTCTTCCCTC
    CAGCTGGAGT TGTCAAAGCT GGACAAGGCA GCCTCTCACC TCCGGCAGCT GACGGATGCA
    TGTCCCAGCC CCAAGGAGCT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe39498
    Clone ID Related Accession (Same CDS sequence) XM_014118300.2 , XM_014118300.1
    Accession Version XM_014118300.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3282bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product valine--tRNA ligase, mitochondrial isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006594.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014118300.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCTGCGGG TCTGGAGCGC CTCTCCGCCT TTGCAGACAC TCCTGATGCC TCGTTCGCCC 
    TTGGCCTCGC TTCCATCACG GTGCTGGGGG CTCGGGCCCT CTCAGGCCCT CCGCAGGTCC
    CACCCTCTCT CTACCCAGTC AGAGCCGTGC CGGTCCTCCC TCTCCCGGAG GAACCATGAA
    GCCAAACAGA AGCGCCAGCG GGAGAAGCAG ATGGCGCTGG AGGCTGGGAT CACCCCGAAG
    GGCAAGGCAC CTGCAGGATC CAGTAAGGCC TGGACTCCTA AGGAGGTAGT GTTGTATGAA
    GTCCCCACGG AACCGGGTGA AAAGAAAGAT GTGTACCGCC TCCTGCCTCC TGCATACAGT
    CCTCGCTACG TTGAGGCGGC CTGGTACTCC TGGTGGGTGC GAGAGGGCTT CTTCAAACCG
    GAGTATCAGA CCAGGCTGCC CCAGGCCACA GGGGAGACGT TTTCCATGTG TATCCCACCT
    CCCAATGTCA CTGGCTCCCT GCATATCGGT CATGCACTGA CAGTAGCCAT ACAGGATGCC
    CTGGTGCGCT GGCACCGGAT GCGTGGGGAT CAGGTGCTAT GGATCCCTGG CTCAGATCAT
    GCAGGAATTG CTACACAAGC TGTGGTGGAG AAGCAACTGT GGAAGGAGCG AGGAATGAGG
    AGACATGAAC TGAGCCGGGA AAAATTCCTT AGGGAGGTGT GGAAGTGGAA GGACGAGAAA
    GGTGGAGAGA TCAGTGAGCA GCTCCAAGCT CTGGGGGCCT CCCTGGACTG GGACCGAGAG
    TGCTTCACTA TGGATGCTGG CTTCTCAGTG GCCGTGACCG AAGCTTTTGT GCGACTCTAC
    GAGGCAGGAT TGTTGTACCG GAGCCAGCAG CTTGTCAACT GGTCCTGCAC TTTAAGATCA
    GCCATCTCGG ATATTGAGGT GGAGAGCCGG CCCCTGCCTG GCCACACGGA GCTTCAACTG
    CCTGGCTGCC CCACTCCTGT GTCTTTTGGC CTCCTTGTGT CTGTGGCCTT CCCTGTGGAT
    GGAGAGCCTG ATGCAGAGGT CGTGGTAGGA ACTACAAGGC CAGAGACGTT GCCTGGAGAC
    GTTGCTGTGG CTGTTCATCC CGACGACTCC CGATACACAC ACCTGCATGG GCGACAGCTT
    CGTCACCCCT TGACTGGGCA GCTTCTCCCC CTCATCACAG ACTCCACTGT TCAGCCACAT
    GTGGGCACAG GGGCAGTGAA GGTGACTCCA GCGCATAGCC CTGCTGATGC TGAGCTGGGG
    GCCCGACACG GCTTGAGCCC TCTGAGTGTC ATCGCGGAGG ATGGCACCAT GACCTCTCTC
    TGTGGGGACT GGCTACAGGG TCTTCACCGA TTTGTGGCCC GGGAAAAGAT TATATCTGCG
    CTGCGGGAGC GGGGCCTGTT CCGGGGCCTC CAGAACCACC CCATGGTGCT TCCTATTTGC
    AGCCGTTCTG GGGACGTGAT AGAATATCTA CTGAAGAGCC AGTGGTTTGT CCGCTGCCGG
    GAGATGGGGG ACCGAGCTGC CCAGATGCAA GTGAGGTGCA GGAAGGGAAG CTCTAACGGC
    CTTTCTGTTC AGGCCGTGGA GTCAGGGGCT CTGGAGCTCA GTCCATCCTT CCACCAGAAG
    AACTGGCAGC ACTGGTTTTC CCACATCGGG GACTGGTGTG TCTCCCGGCA GCTGTGGTGG
    GGCCATCGGA TTCCCGCCTA CCTGGTTGTA GAGGAGCACA TGAAGGGTGA TGGAGAAGAC
    TGTTGGGTGG TTGGGCGGAC AGAGACCGAG GCCAGAAAAA TAGCTGCAGA ACTGACTGGG
    AGATCAGAGG CAGAGCTGAC CCTGCAGAGG GATCCTGATG TCCTGGACAC ATGGTTCTCT
    TCGGCTCTTT TCCCCTTTGC TGCCTTGGGC TGGCCCCAAG AGACCCCAGA CCTTGCTCGT
    TTCTACCCCC TGTCACTTTT GGAAACAGGC AGTGATCTCC TGTTGTTCTG GGTGGGCCGC
    ATGGTCATGT TGGGGACACA GCTCACAGGG CAGCTCCCCT TCACCAAGGT GCTTCTTCAT
    TCCATGGTTC GGGACGGGCA GGGCCGGAAG ATGAGCAAGT CCCTGGGGAA TGTATTGGAC
    CCACGAGACA TCATCAGTGG GGTGGAACTG CAGGTGCTGC AGGAAAAGCT GAAGGACGGG
    AACTTGGACT CCACAGAGCT GGCGATTGCG GCTGCGGCAC AGAGGAAGGA CTTCCCTCAT
    GGGATCCCTG AGTGTGGGAC AGATGCCCTG CGATTTGCCC TGTGCTCCCA CAGGGCCTTG
    GGGGGCGATG TACACTTGTC CGTCTCTGAG GTCTTGAGTT CCCGGCATTT CTGCAACAAA
    ATCTGGAATG CCCTGCGTTT TATCCTCAAT GCCTTAGGAG AGGGATTTGT ACCGCAGCCT
    GCAGAGGAGC TATCCCCCTC ATCCCCCACG GACGCCTGGA TCCTGAGCCG TCTGGCCCAC
    ACTGCCCAGG AGTGTGAGCG AGCCTTCCTT GCTCGGGAGC TGCCACTCAT CACCCATGCC
    CTCCACCACT TCTGGCTCCA CAGCCTCTGT GATGTCTACT TGGAAGCTGT GAAGCCAGTG
    CTTTCGCACT CGCCCCATCC CCAAGGCCCG CCTCAGGTCC TGTTCTCCTG CGCTGATGTG
    GGTCTCCGCC TCCTCGCCCC ACTGATGCCC TTCCTGGCTG AAGAGCTCTG GCAGAGGCTG
    CCACCCAGGC CTGGCAGCAA CTCTGCCCCT AGCATCTCTG TTGCTCCCTA CCCCACCACC
    CATAGCTTGG AGCACTGGCG CCAGCCTGCG CTGGAACAAC ACTTCTCTCA GGTCCAGGAG
    GCTGTGCAGG TACTGAGGGC CCTCCGAGCC ACGTACCAGC TCACCAAGGC CCGGCCCCGA
    GTACTGCTGC AGAGCTCAGA GCCTGGAGAG CTGAGCCTCT TCAAGCCCTT CCTGGAGCCC
    TTGGCCACAC TGGGCCACTG TGGGGCCGTG GGCCTGTTAC CCCCAGGTGT AGCGGCACCC
    TCCGGCTGGG CTCAGGCCCC ACTCAGTGAC ACCATTCAGG TGTACATGGA GCTACAGGGC
    CTGGTGGACC CCCAGGCCCA TCTGGCTCTG CTAGCTGCCC GAAGACGCAA GTTGCAGAAG
    CAGCTGGATG GCCTCATGGC CTGGACCCCA TCAGGGGGAG AGGAAGAGAC TAAGAGGCAG
    CAGAGGCTTT CTTCCCTCCA GCTGGAGTTG TCAAAGCTGG ACAAGGCAGC CTCTCACCTC
    CGGCAGCTGA CGGATGCATG TCCCAGCCCC AAGGAGCTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013973775.1
    CDS101..3382
    Translation

    Target ORF information:

    RefSeq Version XM_014118300.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014118300.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCTGCGGG TCTGGAGCGC CTCTCCGCCT TTGCAGACAC TCCTGATGCC TCGTTCGCCC 
    TTGGCCTCGC TTCCATCACG GTGCTGGGGG CTCGGGCCCT CTCAGGCCCT CCGCAGGTCC
    CACCCTCTCT CTACCCAGTC AGAGCCGTGC CGGTCCTCCC TCTCCCGGAG GAACCATGAA
    GCCAAACAGA AGCGCCAGCG GGAGAAGCAG ATGGCGCTGG AGGCTGGGAT CACCCCGAAG
    GGCAAGGCAC CTGCAGGATC CAGTAAGGCC TGGACTCCTA AGGAGGTAGT GTTGTATGAA
    GTCCCCACGG AACCGGGTGA AAAGAAAGAT GTGTACCGCC TCCTGCCTCC TGCATACAGT
    CCTCGCTACG TTGAGGCGGC CTGGTACTCC TGGTGGGTGC GAGAGGGCTT CTTCAAACCG
    GAGTATCAGA CCAGGCTGCC CCAGGCCACA GGGGAGACGT TTTCCATGTG TATCCCACCT
    CCCAATGTCA CTGGCTCCCT GCATATCGGT CATGCACTGA CAGTAGCCAT ACAGGATGCC
    CTGGTGCGCT GGCACCGGAT GCGTGGGGAT CAGGTGCTAT GGATCCCTGG CTCAGATCAT
    GCAGGAATTG CTACACAAGC TGTGGTGGAG AAGCAACTGT GGAAGGAGCG AGGAATGAGG
    AGACATGAAC TGAGCCGGGA AAAATTCCTT AGGGAGGTGT GGAAGTGGAA GGACGAGAAA
    GGTGGAGAGA TCAGTGAGCA GCTCCAAGCT CTGGGGGCCT CCCTGGACTG GGACCGAGAG
    TGCTTCACTA TGGATGCTGG CTTCTCAGTG GCCGTGACCG AAGCTTTTGT GCGACTCTAC
    GAGGCAGGAT TGTTGTACCG GAGCCAGCAG CTTGTCAACT GGTCCTGCAC TTTAAGATCA
    GCCATCTCGG ATATTGAGGT GGAGAGCCGG CCCCTGCCTG GCCACACGGA GCTTCAACTG
    CCTGGCTGCC CCACTCCTGT GTCTTTTGGC CTCCTTGTGT CTGTGGCCTT CCCTGTGGAT
    GGAGAGCCTG ATGCAGAGGT CGTGGTAGGA ACTACAAGGC CAGAGACGTT GCCTGGAGAC
    GTTGCTGTGG CTGTTCATCC CGACGACTCC CGATACACAC ACCTGCATGG GCGACAGCTT
    CGTCACCCCT TGACTGGGCA GCTTCTCCCC CTCATCACAG ACTCCACTGT TCAGCCACAT
    GTGGGCACAG GGGCAGTGAA GGTGACTCCA GCGCATAGCC CTGCTGATGC TGAGCTGGGG
    GCCCGACACG GCTTGAGCCC TCTGAGTGTC ATCGCGGAGG ATGGCACCAT GACCTCTCTC
    TGTGGGGACT GGCTACAGGG TCTTCACCGA TTTGTGGCCC GGGAAAAGAT TATATCTGCG
    CTGCGGGAGC GGGGCCTGTT CCGGGGCCTC CAGAACCACC CCATGGTGCT TCCTATTTGC
    AGCCGTTCTG GGGACGTGAT AGAATATCTA CTGAAGAGCC AGTGGTTTGT CCGCTGCCGG
    GAGATGGGGG ACCGAGCTGC CCAGATGCAA GTGAGGTGCA GGAAGGGAAG CTCTAACGGC
    CTTTCTGTTC AGGCCGTGGA GTCAGGGGCT CTGGAGCTCA GTCCATCCTT CCACCAGAAG
    AACTGGCAGC ACTGGTTTTC CCACATCGGG GACTGGTGTG TCTCCCGGCA GCTGTGGTGG
    GGCCATCGGA TTCCCGCCTA CCTGGTTGTA GAGGAGCACA TGAAGGGTGA TGGAGAAGAC
    TGTTGGGTGG TTGGGCGGAC AGAGACCGAG GCCAGAAAAA TAGCTGCAGA ACTGACTGGG
    AGATCAGAGG CAGAGCTGAC CCTGCAGAGG GATCCTGATG TCCTGGACAC ATGGTTCTCT
    TCGGCTCTTT TCCCCTTTGC TGCCTTGGGC TGGCCCCAAG AGACCCCAGA CCTTGCTCGT
    TTCTACCCCC TGTCACTTTT GGAAACAGGC AGTGATCTCC TGTTGTTCTG GGTGGGCCGC
    ATGGTCATGT TGGGGACACA GCTCACAGGG CAGCTCCCCT TCACCAAGGT GCTTCTTCAT
    TCCATGGTTC GGGACGGGCA GGGCCGGAAG ATGAGCAAGT CCCTGGGGAA TGTATTGGAC
    CCACGAGACA TCATCAGTGG GGTGGAACTG CAGGTGCTGC AGGAAAAGCT GAAGGACGGG
    AACTTGGACT CCACAGAGCT GGCGATTGCG GCTGCGGCAC AGAGGAAGGA CTTCCCTCAT
    GGGATCCCTG AGTGTGGGAC AGATGCCCTG CGATTTGCCC TGTGCTCCCA CAGGGCCTTG
    GGGGGCGATG TACACTTGTC CGTCTCTGAG GTCTTGAGTT CCCGGCATTT CTGCAACAAA
    ATCTGGAATG CCCTGCGTTT TATCCTCAAT GCCTTAGGAG AGGGATTTGT ACCGCAGCCT
    GCAGAGGAGC TATCCCCCTC ATCCCCCACG GACGCCTGGA TCCTGAGCCG TCTGGCCCAC
    ACTGCCCAGG AGTGTGAGCG AGCCTTCCTT GCTCGGGAGC TGCCACTCAT CACCCATGCC
    CTCCACCACT TCTGGCTCCA CAGCCTCTGT GATGTCTACT TGGAAGCTGT GAAGCCAGTG
    CTTTCGCACT CGCCCCATCC CCAAGGCCCG CCTCAGGTCC TGTTCTCCTG CGCTGATGTG
    GGTCTCCGCC TCCTCGCCCC ACTGATGCCC TTCCTGGCTG AAGAGCTCTG GCAGAGGCTG
    CCACCCAGGC CTGGCAGCAA CTCTGCCCCT AGCATCTCTG TTGCTCCCTA CCCCACCACC
    CATAGCTTGG AGCACTGGCG CCAGCCTGCG CTGGAACAAC ACTTCTCTCA GGTCCAGGAG
    GCTGTGCAGG TACTGAGGGC CCTCCGAGCC ACGTACCAGC TCACCAAGGC CCGGCCCCGA
    GTACTGCTGC AGAGCTCAGA GCCTGGAGAG CTGAGCCTCT TCAAGCCCTT CCTGGAGCCC
    TTGGCCACAC TGGGCCACTG TGGGGCCGTG GGCCTGTTAC CCCCAGGTGT AGCGGCACCC
    TCCGGCTGGG CTCAGGCCCC ACTCAGTGAC ACCATTCAGG TGTACATGGA GCTACAGGGC
    CTGGTGGACC CCCAGGCCCA TCTGGCTCTG CTAGCTGCCC GAAGACGCAA GTTGCAGAAG
    CAGCTGGATG GCCTCATGGC CTGGACCCCA TCAGGGGGAG AGGAAGAGAC TAAGAGGCAG
    CAGAGGCTTT CTTCCCTCCA GCTGGAGTTG TCAAAGCTGG ACAAGGCAGC CTCTCACCTC
    CGGCAGCTGA CGGATGCATG TCCCAGCCCC AAGGAGCTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe39498
    Clone ID Related Accession (Same CDS sequence) XM_014118300.2 , XM_014118300.1
    Accession Version XM_014118300.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3282bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product valine--tRNA ligase, mitochondrial isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726081.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCTGCGGG TCTGGAGCGC CTCTCCGCCT TTGCAGACAC TCCTGATGCC TCGTTCGCCC 
    TTGGCCTCGC TTCCATCACG GTGCTGGGGG CTCGGGCCCT CTCAGGCCCT CCGCAGGTCC
    CACCCTCTCT CTACCCAGTC AGAGCCGTGC CGGTCCTCCC TCTCCCGGAG GAACCATGAA
    GCCAAACAGA AGCGCCAGCG GGAGAAGCAG ATGGCGCTGG AGGCTGGGAT CACCCCGAAG
    GGCAAGGCAC CTGCAGGATC CAGTAAGGCC TGGACTCCTA AGGAGGTAGT GTTGTATGAA
    GTCCCCACGG AACCGGGTGA AAAGAAAGAT GTGTACCGCC TCCTGCCTCC TGCATACAGT
    CCTCGCTACG TTGAGGCGGC CTGGTACTCC TGGTGGGTGC GAGAGGGCTT CTTCAAACCG
    GAGTATCAGA CCAGGCTGCC CCAGGCCACA GGGGAGACGT TTTCCATGTG TATCCCACCT
    CCCAATGTCA CTGGCTCCCT GCATATCGGT CATGCACTGA CAGTAGCCAT ACAGGATGCC
    CTGGTGCGCT GGCACCGGAT GCGTGGGGAT CAGGTGCTAT GGATCCCTGG CTCAGATCAT
    GCAGGAATTG CTACACAAGC TGTGGTGGAG AAGCAACTGT GGAAGGAGCG AGGAATGAGG
    AGACATGAAC TGAGCCGGGA AAAATTCCTT AGGGAGGTGT GGAAGTGGAA GGACGAGAAA
    GGTGGAGAGA TCAGTGAGCA GCTCCAAGCT CTGGGGGCCT CCCTGGACTG GGACCGAGAG
    TGCTTCACTA TGGATGCTGG CTTCTCAGTG GCCGTGACCG AAGCTTTTGT GCGACTCTAC
    GAGGCAGGAT TGTTGTACCG GAGCCAGCAG CTTGTCAACT GGTCCTGCAC TTTAAGATCA
    GCCATCTCGG ATATTGAGGT GGAGAGCCGG CCCCTGCCTG GCCACACGGA GCTTCAACTG
    CCTGGCTGCC CCACTCCTGT GTCTTTTGGC CTCCTTGTGT CTGTGGCCTT CCCTGTGGAT
    GGAGAGCCTG ATGCAGAGGT CGTGGTAGGA ACTACAAGGC CAGAGACGTT GCCTGGAGAC
    GTTGCTGTGG CTGTTCATCC CGACGACTCC CGATACACAC ACCTGCATGG GCGACAGCTT
    CGTCACCCCT TGACTGGGCA GCTTCTCCCC CTCATCACAG ACTCCACTGT TCAGCCACAT
    GTGGGCACAG GGGCAGTGAA GGTGACTCCA GCGCATAGCC CTGCTGATGC TGAGCTGGGG
    GCCCGACACG GCTTGAGCCC TCTGAGTGTC ATCGCGGAGG ATGGCACCAT GACCTCTCTC
    TGTGGGGACT GGCTACAGGG TCTTCACCGA TTTGTGGCCC GGGAAAAGAT TATATCTGCG
    CTGCGGGAGC GGGGCCTGTT CCGGGGCCTC CAGAACCACC CCATGGTGCT TCCTATTTGC
    AGCCGTTCTG GGGACGTGAT AGAATATCTA CTGAAGAGCC AGTGGTTTGT CCGCTGCCGG
    GAGATGGGGG ACCGAGCTGC CCAGATGCAA GTGAGGTGCA GGAAGGGAAG CTCTAACGGC
    CTTTCTGTTC AGGCCGTGGA GTCAGGGGCT CTGGAGCTCA GTCCATCCTT CCACCAGAAG
    AACTGGCAGC ACTGGTTTTC CCACATCGGG GACTGGTGTG TCTCCCGGCA GCTGTGGTGG
    GGCCATCGGA TTCCCGCCTA CCTGGTTGTA GAGGAGCACA TGAAGGGTGA TGGAGAAGAC
    TGTTGGGTGG TTGGGCGGAC AGAGACCGAG GCCAGAAAAA TAGCTGCAGA ACTGACTGGG
    AGATCAGAGG CAGAGCTGAC CCTGCAGAGG GATCCTGATG TCCTGGACAC ATGGTTCTCT
    TCGGCTCTTT TCCCCTTTGC TGCCTTGGGC TGGCCCCAAG AGACCCCAGA CCTTGCTCGT
    TTCTACCCCC TGTCACTTTT GGAAACAGGC AGTGATCTCC TGTTGTTCTG GGTGGGCCGC
    ATGGTCATGT TGGGGACACA GCTCACAGGG CAGCTCCCCT TCACCAAGGT GCTTCTTCAT
    TCCATGGTTC GGGACGGGCA GGGCCGGAAG ATGAGCAAGT CCCTGGGGAA TGTATTGGAC
    CCACGAGACA TCATCAGTGG GGTGGAACTG CAGGTGCTGC AGGAAAAGCT GAAGGACGGG
    AACTTGGACT CCACAGAGCT GGCGATTGCG GCTGCGGCAC AGAGGAAGGA CTTCCCTCAT
    GGGATCCCTG AGTGTGGGAC AGATGCCCTG CGATTTGCCC TGTGCTCCCA CAGGGCCTTG
    GGGGGCGATG TACACTTGTC CGTCTCTGAG GTCTTGAGTT CCCGGCATTT CTGCAACAAA
    ATCTGGAATG CCCTGCGTTT TATCCTCAAT GCCTTAGGAG AGGGATTTGT ACCGCAGCCT
    GCAGAGGAGC TATCCCCCTC ATCCCCCACG GACGCCTGGA TCCTGAGCCG TCTGGCCCAC
    ACTGCCCAGG AGTGTGAGCG AGCCTTCCTT GCTCGGGAGC TGCCACTCAT CACCCATGCC
    CTCCACCACT TCTGGCTCCA CAGCCTCTGT GATGTCTACT TGGAAGCTGT GAAGCCAGTG
    CTTTCGCACT CGCCCCATCC CCAAGGCCCG CCTCAGGTCC TGTTCTCCTG CGCTGATGTG
    GGTCTCCGCC TCCTCGCCCC ACTGATGCCC TTCCTGGCTG AAGAGCTCTG GCAGAGGCTG
    CCACCCAGGC CTGGCAGCAA CTCTGCCCCT AGCATCTCTG TTGCTCCCTA CCCCACCACC
    CATAGCTTGG AGCACTGGCG CCAGCCTGCG CTGGAACAAC ACTTCTCTCA GGTCCAGGAG
    GCTGTGCAGG TACTGAGGGC CCTCCGAGCC ACGTACCAGC TCACCAAGGC CCGGCCCCGA
    GTACTGCTGC AGAGCTCAGA GCCTGGAGAG CTGAGCCTCT TCAAGCCCTT CCTGGAGCCC
    TTGGCCACAC TGGGCCACTG TGGGGCCGTG GGCCTGTTAC CCCCAGGTGT AGCGGCACCC
    TCCGGCTGGG CTCAGGCCCC ACTCAGTGAC ACCATTCAGG TGTACATGGA GCTACAGGGC
    CTGGTGGACC CCCAGGCCCA TCTGGCTCTG CTAGCTGCCC GAAGACGCAA GTTGCAGAAG
    CAGCTGGATG GCCTCATGGC CTGGACCCCA TCAGGGGGAG AGGAAGAGAC TAAGAGGCAG
    CAGAGGCTTT CTTCCCTCCA GCTGGAGTTG TCAAAGCTGG ACAAGGCAGC CTCTCACCTC
    CGGCAGCTGA CGGATGCATG TCCCAGCCCC AAGGAGCTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013973775.1
    CDS131..3412
    Translation

    Target ORF information:

    RefSeq Version XM_014118300.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014118300.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCTGCGGG TCTGGAGCGC CTCTCCGCCT TTGCAGACAC TCCTGATGCC TCGTTCGCCC 
    TTGGCCTCGC TTCCATCACG GTGCTGGGGG CTCGGGCCCT CTCAGGCCCT CCGCAGGTCC
    CACCCTCTCT CTACCCAGTC AGAGCCGTGC CGGTCCTCCC TCTCCCGGAG GAACCATGAA
    GCCAAACAGA AGCGCCAGCG GGAGAAGCAG ATGGCGCTGG AGGCTGGGAT CACCCCGAAG
    GGCAAGGCAC CTGCAGGATC CAGTAAGGCC TGGACTCCTA AGGAGGTAGT GTTGTATGAA
    GTCCCCACGG AACCGGGTGA AAAGAAAGAT GTGTACCGCC TCCTGCCTCC TGCATACAGT
    CCTCGCTACG TTGAGGCGGC CTGGTACTCC TGGTGGGTGC GAGAGGGCTT CTTCAAACCG
    GAGTATCAGA CCAGGCTGCC CCAGGCCACA GGGGAGACGT TTTCCATGTG TATCCCACCT
    CCCAATGTCA CTGGCTCCCT GCATATCGGT CATGCACTGA CAGTAGCCAT ACAGGATGCC
    CTGGTGCGCT GGCACCGGAT GCGTGGGGAT CAGGTGCTAT GGATCCCTGG CTCAGATCAT
    GCAGGAATTG CTACACAAGC TGTGGTGGAG AAGCAACTGT GGAAGGAGCG AGGAATGAGG
    AGACATGAAC TGAGCCGGGA AAAATTCCTT AGGGAGGTGT GGAAGTGGAA GGACGAGAAA
    GGTGGAGAGA TCAGTGAGCA GCTCCAAGCT CTGGGGGCCT CCCTGGACTG GGACCGAGAG
    TGCTTCACTA TGGATGCTGG CTTCTCAGTG GCCGTGACCG AAGCTTTTGT GCGACTCTAC
    GAGGCAGGAT TGTTGTACCG GAGCCAGCAG CTTGTCAACT GGTCCTGCAC TTTAAGATCA
    GCCATCTCGG ATATTGAGGT GGAGAGCCGG CCCCTGCCTG GCCACACGGA GCTTCAACTG
    CCTGGCTGCC CCACTCCTGT GTCTTTTGGC CTCCTTGTGT CTGTGGCCTT CCCTGTGGAT
    GGAGAGCCTG ATGCAGAGGT CGTGGTAGGA ACTACAAGGC CAGAGACGTT GCCTGGAGAC
    GTTGCTGTGG CTGTTCATCC CGACGACTCC CGATACACAC ACCTGCATGG GCGACAGCTT
    CGTCACCCCT TGACTGGGCA GCTTCTCCCC CTCATCACAG ACTCCACTGT TCAGCCACAT
    GTGGGCACAG GGGCAGTGAA GGTGACTCCA GCGCATAGCC CTGCTGATGC TGAGCTGGGG
    GCCCGACACG GCTTGAGCCC TCTGAGTGTC ATCGCGGAGG ATGGCACCAT GACCTCTCTC
    TGTGGGGACT GGCTACAGGG TCTTCACCGA TTTGTGGCCC GGGAAAAGAT TATATCTGCG
    CTGCGGGAGC GGGGCCTGTT CCGGGGCCTC CAGAACCACC CCATGGTGCT TCCTATTTGC
    AGCCGTTCTG GGGACGTGAT AGAATATCTA CTGAAGAGCC AGTGGTTTGT CCGCTGCCGG
    GAGATGGGGG ACCGAGCTGC CCAGATGCAA GTGAGGTGCA GGAAGGGAAG CTCTAACGGC
    CTTTCTGTTC AGGCCGTGGA GTCAGGGGCT CTGGAGCTCA GTCCATCCTT CCACCAGAAG
    AACTGGCAGC ACTGGTTTTC CCACATCGGG GACTGGTGTG TCTCCCGGCA GCTGTGGTGG
    GGCCATCGGA TTCCCGCCTA CCTGGTTGTA GAGGAGCACA TGAAGGGTGA TGGAGAAGAC
    TGTTGGGTGG TTGGGCGGAC AGAGACCGAG GCCAGAAAAA TAGCTGCAGA ACTGACTGGG
    AGATCAGAGG CAGAGCTGAC CCTGCAGAGG GATCCTGATG TCCTGGACAC ATGGTTCTCT
    TCGGCTCTTT TCCCCTTTGC TGCCTTGGGC TGGCCCCAAG AGACCCCAGA CCTTGCTCGT
    TTCTACCCCC TGTCACTTTT GGAAACAGGC AGTGATCTCC TGTTGTTCTG GGTGGGCCGC
    ATGGTCATGT TGGGGACACA GCTCACAGGG CAGCTCCCCT TCACCAAGGT GCTTCTTCAT
    TCCATGGTTC GGGACGGGCA GGGCCGGAAG ATGAGCAAGT CCCTGGGGAA TGTATTGGAC
    CCACGAGACA TCATCAGTGG GGTGGAACTG CAGGTGCTGC AGGAAAAGCT GAAGGACGGG
    AACTTGGACT CCACAGAGCT GGCGATTGCG GCTGCGGCAC AGAGGAAGGA CTTCCCTCAT
    GGGATCCCTG AGTGTGGGAC AGATGCCCTG CGATTTGCCC TGTGCTCCCA CAGGGCCTTG
    GGGGGCGATG TACACTTGTC CGTCTCTGAG GTCTTGAGTT CCCGGCATTT CTGCAACAAA
    ATCTGGAATG CCCTGCGTTT TATCCTCAAT GCCTTAGGAG AGGGATTTGT ACCGCAGCCT
    GCAGAGGAGC TATCCCCCTC ATCCCCCACG GACGCCTGGA TCCTGAGCCG TCTGGCCCAC
    ACTGCCCAGG AGTGTGAGCG AGCCTTCCTT GCTCGGGAGC TGCCACTCAT CACCCATGCC
    CTCCACCACT TCTGGCTCCA CAGCCTCTGT GATGTCTACT TGGAAGCTGT GAAGCCAGTG
    CTTTCGCACT CGCCCCATCC CCAAGGCCCG CCTCAGGTCC TGTTCTCCTG CGCTGATGTG
    GGTCTCCGCC TCCTCGCCCC ACTGATGCCC TTCCTGGCTG AAGAGCTCTG GCAGAGGCTG
    CCACCCAGGC CTGGCAGCAA CTCTGCCCCT AGCATCTCTG TTGCTCCCTA CCCCACCACC
    CATAGCTTGG AGCACTGGCG CCAGCCTGCG CTGGAACAAC ACTTCTCTCA GGTCCAGGAG
    GCTGTGCAGG TACTGAGGGC CCTCCGAGCC ACGTACCAGC TCACCAAGGC CCGGCCCCGA
    GTACTGCTGC AGAGCTCAGA GCCTGGAGAG CTGAGCCTCT TCAAGCCCTT CCTGGAGCCC
    TTGGCCACAC TGGGCCACTG TGGGGCCGTG GGCCTGTTAC CCCCAGGTGT AGCGGCACCC
    TCCGGCTGGG CTCAGGCCCC ACTCAGTGAC ACCATTCAGG TGTACATGGA GCTACAGGGC
    CTGGTGGACC CCCAGGCCCA TCTGGCTCTG CTAGCTGCCC GAAGACGCAA GTTGCAGAAG
    CAGCTGGATG GCCTCATGGC CTGGACCCCA TCAGGGGGAG AGGAAGAGAC TAAGAGGCAG
    CAGAGGCTTT CTTCCCTCCA GCTGGAGTTG TCAAAGCTGG ACAAGGCAGC CTCTCACCTC
    CGGCAGCTGA CGGATGCATG TCCCAGCCCC AAGGAGCTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe39499
    Clone ID Related Accession (Same CDS sequence) XM_014118301.1 , XM_014118301.2
    Accession Version XM_014118301.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3276bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product valine--tRNA ligase, mitochondrial isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726081.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCTGCGGG TCTGGAGCGC CTCTCCGCCT TTGCAGACAC TCCTGATGCC TCGTTCGCCC 
    TTGGCCTCGC TTCCATCACG GTGCTGGGGG CTCGGGCCCT CTCAGGCCCT CCGCAGGTCC
    CACCCTCTCT CTACCCAGTC AGAGCCGTGC CGGTCCTCCC TCTCCCGGAG GAACCATGAA
    GCCAAACAGA AGCGCCAGCG GGAGAAGCAG ATGGCGCTGG AGGCTGGGAT CACCCCGAAG
    GGCAAGGCAC CTGCAGGATC CAGTAAGGCC TGGACTCCTA AGGAGGTAGT GTTGTATGAA
    GTCCCCACGG AACCGGGTGA AAAGAAAGAT GTGTACCGCC TCCTGCCTCC TGCATACAGT
    CCTCGCTACG TTGAGGCGGC CTGGTACTCC TGGTGGGTGC GAGAGGGCTT CTTCAAACCG
    GAGTATCAGA CCAGGCTGCC CCAGGCCACA GGGGAGACGT TTTCCATGTG TATCCCACCT
    CCCAATGTCA CTGGCTCCCT GCATATCGGT CATGCACTGA CAGTAGCCAT ACAGGATGCC
    CTGGTGCGCT GGCACCGGAT GCGTGGGGAT CAGGTGCTAT GGATCCCTGG CTCAGATCAT
    GCAGGAATTG CTACACAAGC TGTGGTGGAG AAGCAACTGT GGAAGGAGCG AGGAATGAGG
    AGACATGAAC TGAGCCGGGA AAAATTCCTT AGGGAGGTGT GGAAGTGGAA GGACGAGAAA
    GGTGGAGAGA TCAGTGAGCA GCTCCAAGCT CTGGGGGCCT CCCTGGACTG GGACCGAGAG
    TGCTTCACTA TGGATGCTGG CTTCTCAGTG GCCGTGACCG AAGCTTTTGT GCGACTCTAC
    GAGGCAGGAT TGTTGTACCG GAGCCAGCAG CTTGTCAACT GGTCCTGCAC TTTAAGATCA
    GCCATCTCGG ATATTGAGGT GGAGAGCCGG CCCCTGCCTG GCCACACGGA GCTTCAACTG
    CCTGGCTGCC CCACTCCTGT GTCTTTTGGC CTCCTTGTGT CTGTGGCCTT CCCTGTGGAT
    GGAGAGCCTG ATGCAGAGGT CGTGGTAGGA ACTACAAGGC CAGAGACGTT GCCTGGAGAC
    GTTGCTGTGG CTGTTCATCC CGACGACTCC CGATACACAC ACCTGCATGG GCGACAGCTT
    CGTCACCCCT TGACTGGGCA GCTTCTCCCC CTCATCACAG ACTCCACTGT TCAGCCACAT
    GTGGGCACAG GGGCAGTGAA GGTGACTCCA GCGCATAGCC CTGCTGATGC TGAGCTGGGG
    GCCCGACACG GCTTGAGCCC TCTGAGTGTC ATCGCGGAGG ATGGCACCAT GACCTCTCTC
    TGTGGGGACT GGCTACAGGG TCTTCACCGA TTTGTGGCCC GGGAAAAGAT TATATCTGCG
    CTGCGGGAGC GGGGCCTGTT CCGGGGCCTC CAGAACCACC CCATGGTGCT TCCTATTTGC
    AGCCGTTCTG GGGACGTGAT AGAATATCTA CTGAAGAGCC AGTGGTTTGT CCGCTGCCGG
    GAGATGGGGG ACCGAGCTGC CCAGGCCGTG GAGTCAGGGG CTCTGGAGCT CAGTCCATCC
    TTCCACCAGA AGAACTGGCA GCACTGGTTT TCCCACATCG GGGACTGGTG TGTCTCCCGG
    CAGCTGTGGT GGGGCCATCG GATTCCCGCC TACCTGGTTG TAGAGGAGCA CATGAAGGGT
    GATGGAGAAG ACTGTTGGGT GGTTGGGCGG ACAGAGACCG AGGCCAGAAA AATAGCTGCA
    GAACTGACTG GGAGATCAGA GGCAGAGCTG ACCCTGCAGA GGGATCCTGA TGTCCTGGAC
    ACATGGTTCT CTTCGGCTCT TTTCCCCTTT GCTGCCTTGG GCTGGCCCCA AGAGACCCCA
    GACCTTGCTC GTTTCTACCC CCTGTCACTT TTGGAAACAG GCAGTGATCT CCTGTTGTTC
    TGGGTGGGCC GCATGGTCAT GTTGGGGACA CAGCTCACAG GGCAGCTCCC CTTCACCAAG
    GTGCTTCTTC ATTCCATGGT TCGGGACGGG CAGGGCCGGA AGATGAGCAA GTCCCTGGGG
    AATGTATTGG ACCCACGAGA CATCATCAGT GGGGTGGAAC TGCAGGTGCT GCAGGAAAAG
    CTGAAGGACG GGAACTTGGA CTCCACAGAG CTGGCGATTG CGGCTGCGGC ACAGCTGTGT
    AGGCATCTGC CACCCTTCTT ATCCCTCTGG TTGCAGAGGA AGGACTTCCC TCATGGGATC
    CCTGAGTGTG GGACAGATGC CCTGCGATTT GCCCTGTGCT CCCACAGGGC CTTGGGGGGC
    GATGTACACT TGTCCGTCTC TGAGGTCTTG AGTTCCCGGC ATTTCTGCAA CAAAATCTGG
    AATGCCCTGC GTTTTATCCT CAATGCCTTA GGAGAGGGAT TTGTACCGCA GCCTGCAGAG
    GAGCTATCCC CCTCATCCCC CACGGACGCC TGGATCCTGA GCCGTCTGGC CCACACTGCC
    CAGGAGTGTG AGCGAGCCTT CCTTGCTCGG GAGCTGCCAC TCATCACCCA TGCCCTCCAC
    CACTTCTGGC TCCACAGCCT CTGTGATGTC TACTTGGAAG CTGTGAAGCC AGTGCTTTCG
    CACTCGCCCC ATCCCCAAGG CCCGCCTCAG GTCCTGTTCT CCTGCGCTGA TGTGGGTCTC
    CGCCTCCTCG CCCCACTGAT GCCCTTCCTG GCTGAAGAGC TCTGGCAGAG GCTGCCACCC
    AGGCCTGGCA GCAACTCTGC CCCTAGCATC TCTGTTGCTC CCTACCCCAC CACCCATAGC
    TTGGAGCACT GGCGCCAGCC TGCGCTGGAA CAACACTTCT CTCAGGTCCA GGAGGCTGTG
    CAGGTACTGA GGGCCCTCCG AGCCACGTAC CAGCTCACCA AGGCCCGGCC CCGAGTACTG
    CTGCAGAGCT CAGAGCCTGG AGAGCTGAGC CTCTTCAAGC CCTTCCTGGA GCCCTTGGCC
    ACACTGGGCC ACTGTGGGGC CGTGGGCCTG TTACCCCCAG GTGTAGCGGC ACCCTCCGGC
    TGGGCTCAGG CCCCACTCAG TGACACCATT CAGGTGTACA TGGAGCTACA GGGCCTGGTG
    GACCCCCAGG CCCATCTGGC TCTGCTAGCT GCCCGAAGAC GCAAGTTGCA GAAGCAGCTG
    GATGGCCTCA TGGCCTGGAC CCCATCAGGG GGAGAGGAAG AGACTAAGAG GCAGCAGAGG
    CTTTCTTCCC TCCAGCTGGA GTTGTCAAAG CTGGACAAGG CAGCCTCTCA CCTCCGGCAG
    CTGACGGATG CATGTCCCAG CCCCAAGGAG CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013973776.1
    CDS131..3406
    Translation

    Target ORF information:

    RefSeq Version XM_014118301.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014118301.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCTGCGGG TCTGGAGCGC CTCTCCGCCT TTGCAGACAC TCCTGATGCC TCGTTCGCCC 
    TTGGCCTCGC TTCCATCACG GTGCTGGGGG CTCGGGCCCT CTCAGGCCCT CCGCAGGTCC
    CACCCTCTCT CTACCCAGTC AGAGCCGTGC CGGTCCTCCC TCTCCCGGAG GAACCATGAA
    GCCAAACAGA AGCGCCAGCG GGAGAAGCAG ATGGCGCTGG AGGCTGGGAT CACCCCGAAG
    GGCAAGGCAC CTGCAGGATC CAGTAAGGCC TGGACTCCTA AGGAGGTAGT GTTGTATGAA
    GTCCCCACGG AACCGGGTGA AAAGAAAGAT GTGTACCGCC TCCTGCCTCC TGCATACAGT
    CCTCGCTACG TTGAGGCGGC CTGGTACTCC TGGTGGGTGC GAGAGGGCTT CTTCAAACCG
    GAGTATCAGA CCAGGCTGCC CCAGGCCACA GGGGAGACGT TTTCCATGTG TATCCCACCT
    CCCAATGTCA CTGGCTCCCT GCATATCGGT CATGCACTGA CAGTAGCCAT ACAGGATGCC
    CTGGTGCGCT GGCACCGGAT GCGTGGGGAT CAGGTGCTAT GGATCCCTGG CTCAGATCAT
    GCAGGAATTG CTACACAAGC TGTGGTGGAG AAGCAACTGT GGAAGGAGCG AGGAATGAGG
    AGACATGAAC TGAGCCGGGA AAAATTCCTT AGGGAGGTGT GGAAGTGGAA GGACGAGAAA
    GGTGGAGAGA TCAGTGAGCA GCTCCAAGCT CTGGGGGCCT CCCTGGACTG GGACCGAGAG
    TGCTTCACTA TGGATGCTGG CTTCTCAGTG GCCGTGACCG AAGCTTTTGT GCGACTCTAC
    GAGGCAGGAT TGTTGTACCG GAGCCAGCAG CTTGTCAACT GGTCCTGCAC TTTAAGATCA
    GCCATCTCGG ATATTGAGGT GGAGAGCCGG CCCCTGCCTG GCCACACGGA GCTTCAACTG
    CCTGGCTGCC CCACTCCTGT GTCTTTTGGC CTCCTTGTGT CTGTGGCCTT CCCTGTGGAT
    GGAGAGCCTG ATGCAGAGGT CGTGGTAGGA ACTACAAGGC CAGAGACGTT GCCTGGAGAC
    GTTGCTGTGG CTGTTCATCC CGACGACTCC CGATACACAC ACCTGCATGG GCGACAGCTT
    CGTCACCCCT TGACTGGGCA GCTTCTCCCC CTCATCACAG ACTCCACTGT TCAGCCACAT
    GTGGGCACAG GGGCAGTGAA GGTGACTCCA GCGCATAGCC CTGCTGATGC TGAGCTGGGG
    GCCCGACACG GCTTGAGCCC TCTGAGTGTC ATCGCGGAGG ATGGCACCAT GACCTCTCTC
    TGTGGGGACT GGCTACAGGG TCTTCACCGA TTTGTGGCCC GGGAAAAGAT TATATCTGCG
    CTGCGGGAGC GGGGCCTGTT CCGGGGCCTC CAGAACCACC CCATGGTGCT TCCTATTTGC
    AGCCGTTCTG GGGACGTGAT AGAATATCTA CTGAAGAGCC AGTGGTTTGT CCGCTGCCGG
    GAGATGGGGG ACCGAGCTGC CCAGGCCGTG GAGTCAGGGG CTCTGGAGCT CAGTCCATCC
    TTCCACCAGA AGAACTGGCA GCACTGGTTT TCCCACATCG GGGACTGGTG TGTCTCCCGG
    CAGCTGTGGT GGGGCCATCG GATTCCCGCC TACCTGGTTG TAGAGGAGCA CATGAAGGGT
    GATGGAGAAG ACTGTTGGGT GGTTGGGCGG ACAGAGACCG AGGCCAGAAA AATAGCTGCA
    GAACTGACTG GGAGATCAGA GGCAGAGCTG ACCCTGCAGA GGGATCCTGA TGTCCTGGAC
    ACATGGTTCT CTTCGGCTCT TTTCCCCTTT GCTGCCTTGG GCTGGCCCCA AGAGACCCCA
    GACCTTGCTC GTTTCTACCC CCTGTCACTT TTGGAAACAG GCAGTGATCT CCTGTTGTTC
    TGGGTGGGCC GCATGGTCAT GTTGGGGACA CAGCTCACAG GGCAGCTCCC CTTCACCAAG
    GTGCTTCTTC ATTCCATGGT TCGGGACGGG CAGGGCCGGA AGATGAGCAA GTCCCTGGGG
    AATGTATTGG ACCCACGAGA CATCATCAGT GGGGTGGAAC TGCAGGTGCT GCAGGAAAAG
    CTGAAGGACG GGAACTTGGA CTCCACAGAG CTGGCGATTG CGGCTGCGGC ACAGCTGTGT
    AGGCATCTGC CACCCTTCTT ATCCCTCTGG TTGCAGAGGA AGGACTTCCC TCATGGGATC
    CCTGAGTGTG GGACAGATGC CCTGCGATTT GCCCTGTGCT CCCACAGGGC CTTGGGGGGC
    GATGTACACT TGTCCGTCTC TGAGGTCTTG AGTTCCCGGC ATTTCTGCAA CAAAATCTGG
    AATGCCCTGC GTTTTATCCT CAATGCCTTA GGAGAGGGAT TTGTACCGCA GCCTGCAGAG
    GAGCTATCCC CCTCATCCCC CACGGACGCC TGGATCCTGA GCCGTCTGGC CCACACTGCC
    CAGGAGTGTG AGCGAGCCTT CCTTGCTCGG GAGCTGCCAC TCATCACCCA TGCCCTCCAC
    CACTTCTGGC TCCACAGCCT CTGTGATGTC TACTTGGAAG CTGTGAAGCC AGTGCTTTCG
    CACTCGCCCC ATCCCCAAGG CCCGCCTCAG GTCCTGTTCT CCTGCGCTGA TGTGGGTCTC
    CGCCTCCTCG CCCCACTGAT GCCCTTCCTG GCTGAAGAGC TCTGGCAGAG GCTGCCACCC
    AGGCCTGGCA GCAACTCTGC CCCTAGCATC TCTGTTGCTC CCTACCCCAC CACCCATAGC
    TTGGAGCACT GGCGCCAGCC TGCGCTGGAA CAACACTTCT CTCAGGTCCA GGAGGCTGTG
    CAGGTACTGA GGGCCCTCCG AGCCACGTAC CAGCTCACCA AGGCCCGGCC CCGAGTACTG
    CTGCAGAGCT CAGAGCCTGG AGAGCTGAGC CTCTTCAAGC CCTTCCTGGA GCCCTTGGCC
    ACACTGGGCC ACTGTGGGGC CGTGGGCCTG TTACCCCCAG GTGTAGCGGC ACCCTCCGGC
    TGGGCTCAGG CCCCACTCAG TGACACCATT CAGGTGTACA TGGAGCTACA GGGCCTGGTG
    GACCCCCAGG CCCATCTGGC TCTGCTAGCT GCCCGAAGAC GCAAGTTGCA GAAGCAGCTG
    GATGGCCTCA TGGCCTGGAC CCCATCAGGG GGAGAGGAAG AGACTAAGAG GCAGCAGAGG
    CTTTCTTCCC TCCAGCTGGA GTTGTCAAAG CTGGACAAGG CAGCCTCTCA CCTCCGGCAG
    CTGACGGATG CATGTCCCAG CCCCAAGGAG CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe39499
    Clone ID Related Accession (Same CDS sequence) XM_014118301.1 , XM_014118301.2
    Accession Version XM_014118301.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3276bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product valine--tRNA ligase, mitochondrial isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006594.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014118301.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCTGCGGG TCTGGAGCGC CTCTCCGCCT TTGCAGACAC TCCTGATGCC TCGTTCGCCC 
    TTGGCCTCGC TTCCATCACG GTGCTGGGGG CTCGGGCCCT CTCAGGCCCT CCGCAGGTCC
    CACCCTCTCT CTACCCAGTC AGAGCCGTGC CGGTCCTCCC TCTCCCGGAG GAACCATGAA
    GCCAAACAGA AGCGCCAGCG GGAGAAGCAG ATGGCGCTGG AGGCTGGGAT CACCCCGAAG
    GGCAAGGCAC CTGCAGGATC CAGTAAGGCC TGGACTCCTA AGGAGGTAGT GTTGTATGAA
    GTCCCCACGG AACCGGGTGA AAAGAAAGAT GTGTACCGCC TCCTGCCTCC TGCATACAGT
    CCTCGCTACG TTGAGGCGGC CTGGTACTCC TGGTGGGTGC GAGAGGGCTT CTTCAAACCG
    GAGTATCAGA CCAGGCTGCC CCAGGCCACA GGGGAGACGT TTTCCATGTG TATCCCACCT
    CCCAATGTCA CTGGCTCCCT GCATATCGGT CATGCACTGA CAGTAGCCAT ACAGGATGCC
    CTGGTGCGCT GGCACCGGAT GCGTGGGGAT CAGGTGCTAT GGATCCCTGG CTCAGATCAT
    GCAGGAATTG CTACACAAGC TGTGGTGGAG AAGCAACTGT GGAAGGAGCG AGGAATGAGG
    AGACATGAAC TGAGCCGGGA AAAATTCCTT AGGGAGGTGT GGAAGTGGAA GGACGAGAAA
    GGTGGAGAGA TCAGTGAGCA GCTCCAAGCT CTGGGGGCCT CCCTGGACTG GGACCGAGAG
    TGCTTCACTA TGGATGCTGG CTTCTCAGTG GCCGTGACCG AAGCTTTTGT GCGACTCTAC
    GAGGCAGGAT TGTTGTACCG GAGCCAGCAG CTTGTCAACT GGTCCTGCAC TTTAAGATCA
    GCCATCTCGG ATATTGAGGT GGAGAGCCGG CCCCTGCCTG GCCACACGGA GCTTCAACTG
    CCTGGCTGCC CCACTCCTGT GTCTTTTGGC CTCCTTGTGT CTGTGGCCTT CCCTGTGGAT
    GGAGAGCCTG ATGCAGAGGT CGTGGTAGGA ACTACAAGGC CAGAGACGTT GCCTGGAGAC
    GTTGCTGTGG CTGTTCATCC CGACGACTCC CGATACACAC ACCTGCATGG GCGACAGCTT
    CGTCACCCCT TGACTGGGCA GCTTCTCCCC CTCATCACAG ACTCCACTGT TCAGCCACAT
    GTGGGCACAG GGGCAGTGAA GGTGACTCCA GCGCATAGCC CTGCTGATGC TGAGCTGGGG
    GCCCGACACG GCTTGAGCCC TCTGAGTGTC ATCGCGGAGG ATGGCACCAT GACCTCTCTC
    TGTGGGGACT GGCTACAGGG TCTTCACCGA TTTGTGGCCC GGGAAAAGAT TATATCTGCG
    CTGCGGGAGC GGGGCCTGTT CCGGGGCCTC CAGAACCACC CCATGGTGCT TCCTATTTGC
    AGCCGTTCTG GGGACGTGAT AGAATATCTA CTGAAGAGCC AGTGGTTTGT CCGCTGCCGG
    GAGATGGGGG ACCGAGCTGC CCAGGCCGTG GAGTCAGGGG CTCTGGAGCT CAGTCCATCC
    TTCCACCAGA AGAACTGGCA GCACTGGTTT TCCCACATCG GGGACTGGTG TGTCTCCCGG
    CAGCTGTGGT GGGGCCATCG GATTCCCGCC TACCTGGTTG TAGAGGAGCA CATGAAGGGT
    GATGGAGAAG ACTGTTGGGT GGTTGGGCGG ACAGAGACCG AGGCCAGAAA AATAGCTGCA
    GAACTGACTG GGAGATCAGA GGCAGAGCTG ACCCTGCAGA GGGATCCTGA TGTCCTGGAC
    ACATGGTTCT CTTCGGCTCT TTTCCCCTTT GCTGCCTTGG GCTGGCCCCA AGAGACCCCA
    GACCTTGCTC GTTTCTACCC CCTGTCACTT TTGGAAACAG GCAGTGATCT CCTGTTGTTC
    TGGGTGGGCC GCATGGTCAT GTTGGGGACA CAGCTCACAG GGCAGCTCCC CTTCACCAAG
    GTGCTTCTTC ATTCCATGGT TCGGGACGGG CAGGGCCGGA AGATGAGCAA GTCCCTGGGG
    AATGTATTGG ACCCACGAGA CATCATCAGT GGGGTGGAAC TGCAGGTGCT GCAGGAAAAG
    CTGAAGGACG GGAACTTGGA CTCCACAGAG CTGGCGATTG CGGCTGCGGC ACAGCTGTGT
    AGGCATCTGC CACCCTTCTT ATCCCTCTGG TTGCAGAGGA AGGACTTCCC TCATGGGATC
    CCTGAGTGTG GGACAGATGC CCTGCGATTT GCCCTGTGCT CCCACAGGGC CTTGGGGGGC
    GATGTACACT TGTCCGTCTC TGAGGTCTTG AGTTCCCGGC ATTTCTGCAA CAAAATCTGG
    AATGCCCTGC GTTTTATCCT CAATGCCTTA GGAGAGGGAT TTGTACCGCA GCCTGCAGAG
    GAGCTATCCC CCTCATCCCC CACGGACGCC TGGATCCTGA GCCGTCTGGC CCACACTGCC
    CAGGAGTGTG AGCGAGCCTT CCTTGCTCGG GAGCTGCCAC TCATCACCCA TGCCCTCCAC
    CACTTCTGGC TCCACAGCCT CTGTGATGTC TACTTGGAAG CTGTGAAGCC AGTGCTTTCG
    CACTCGCCCC ATCCCCAAGG CCCGCCTCAG GTCCTGTTCT CCTGCGCTGA TGTGGGTCTC
    CGCCTCCTCG CCCCACTGAT GCCCTTCCTG GCTGAAGAGC TCTGGCAGAG GCTGCCACCC
    AGGCCTGGCA GCAACTCTGC CCCTAGCATC TCTGTTGCTC CCTACCCCAC CACCCATAGC
    TTGGAGCACT GGCGCCAGCC TGCGCTGGAA CAACACTTCT CTCAGGTCCA GGAGGCTGTG
    CAGGTACTGA GGGCCCTCCG AGCCACGTAC CAGCTCACCA AGGCCCGGCC CCGAGTACTG
    CTGCAGAGCT CAGAGCCTGG AGAGCTGAGC CTCTTCAAGC CCTTCCTGGA GCCCTTGGCC
    ACACTGGGCC ACTGTGGGGC CGTGGGCCTG TTACCCCCAG GTGTAGCGGC ACCCTCCGGC
    TGGGCTCAGG CCCCACTCAG TGACACCATT CAGGTGTACA TGGAGCTACA GGGCCTGGTG
    GACCCCCAGG CCCATCTGGC TCTGCTAGCT GCCCGAAGAC GCAAGTTGCA GAAGCAGCTG
    GATGGCCTCA TGGCCTGGAC CCCATCAGGG GGAGAGGAAG AGACTAAGAG GCAGCAGAGG
    CTTTCTTCCC TCCAGCTGGA GTTGTCAAAG CTGGACAAGG CAGCCTCTCA CCTCCGGCAG
    CTGACGGATG CATGTCCCAG CCCCAAGGAG CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013973776.1
    CDS101..3376
    Translation

    Target ORF information:

    RefSeq Version XM_014118301.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014118301.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCTGCGGG TCTGGAGCGC CTCTCCGCCT TTGCAGACAC TCCTGATGCC TCGTTCGCCC 
    TTGGCCTCGC TTCCATCACG GTGCTGGGGG CTCGGGCCCT CTCAGGCCCT CCGCAGGTCC
    CACCCTCTCT CTACCCAGTC AGAGCCGTGC CGGTCCTCCC TCTCCCGGAG GAACCATGAA
    GCCAAACAGA AGCGCCAGCG GGAGAAGCAG ATGGCGCTGG AGGCTGGGAT CACCCCGAAG
    GGCAAGGCAC CTGCAGGATC CAGTAAGGCC TGGACTCCTA AGGAGGTAGT GTTGTATGAA
    GTCCCCACGG AACCGGGTGA AAAGAAAGAT GTGTACCGCC TCCTGCCTCC TGCATACAGT
    CCTCGCTACG TTGAGGCGGC CTGGTACTCC TGGTGGGTGC GAGAGGGCTT CTTCAAACCG
    GAGTATCAGA CCAGGCTGCC CCAGGCCACA GGGGAGACGT TTTCCATGTG TATCCCACCT
    CCCAATGTCA CTGGCTCCCT GCATATCGGT CATGCACTGA CAGTAGCCAT ACAGGATGCC
    CTGGTGCGCT GGCACCGGAT GCGTGGGGAT CAGGTGCTAT GGATCCCTGG CTCAGATCAT
    GCAGGAATTG CTACACAAGC TGTGGTGGAG AAGCAACTGT GGAAGGAGCG AGGAATGAGG
    AGACATGAAC TGAGCCGGGA AAAATTCCTT AGGGAGGTGT GGAAGTGGAA GGACGAGAAA
    GGTGGAGAGA TCAGTGAGCA GCTCCAAGCT CTGGGGGCCT CCCTGGACTG GGACCGAGAG
    TGCTTCACTA TGGATGCTGG CTTCTCAGTG GCCGTGACCG AAGCTTTTGT GCGACTCTAC
    GAGGCAGGAT TGTTGTACCG GAGCCAGCAG CTTGTCAACT GGTCCTGCAC TTTAAGATCA
    GCCATCTCGG ATATTGAGGT GGAGAGCCGG CCCCTGCCTG GCCACACGGA GCTTCAACTG
    CCTGGCTGCC CCACTCCTGT GTCTTTTGGC CTCCTTGTGT CTGTGGCCTT CCCTGTGGAT
    GGAGAGCCTG ATGCAGAGGT CGTGGTAGGA ACTACAAGGC CAGAGACGTT GCCTGGAGAC
    GTTGCTGTGG CTGTTCATCC CGACGACTCC CGATACACAC ACCTGCATGG GCGACAGCTT
    CGTCACCCCT TGACTGGGCA GCTTCTCCCC CTCATCACAG ACTCCACTGT TCAGCCACAT
    GTGGGCACAG GGGCAGTGAA GGTGACTCCA GCGCATAGCC CTGCTGATGC TGAGCTGGGG
    GCCCGACACG GCTTGAGCCC TCTGAGTGTC ATCGCGGAGG ATGGCACCAT GACCTCTCTC
    TGTGGGGACT GGCTACAGGG TCTTCACCGA TTTGTGGCCC GGGAAAAGAT TATATCTGCG
    CTGCGGGAGC GGGGCCTGTT CCGGGGCCTC CAGAACCACC CCATGGTGCT TCCTATTTGC
    AGCCGTTCTG GGGACGTGAT AGAATATCTA CTGAAGAGCC AGTGGTTTGT CCGCTGCCGG
    GAGATGGGGG ACCGAGCTGC CCAGGCCGTG GAGTCAGGGG CTCTGGAGCT CAGTCCATCC
    TTCCACCAGA AGAACTGGCA GCACTGGTTT TCCCACATCG GGGACTGGTG TGTCTCCCGG
    CAGCTGTGGT GGGGCCATCG GATTCCCGCC TACCTGGTTG TAGAGGAGCA CATGAAGGGT
    GATGGAGAAG ACTGTTGGGT GGTTGGGCGG ACAGAGACCG AGGCCAGAAA AATAGCTGCA
    GAACTGACTG GGAGATCAGA GGCAGAGCTG ACCCTGCAGA GGGATCCTGA TGTCCTGGAC
    ACATGGTTCT CTTCGGCTCT TTTCCCCTTT GCTGCCTTGG GCTGGCCCCA AGAGACCCCA
    GACCTTGCTC GTTTCTACCC CCTGTCACTT TTGGAAACAG GCAGTGATCT CCTGTTGTTC
    TGGGTGGGCC GCATGGTCAT GTTGGGGACA CAGCTCACAG GGCAGCTCCC CTTCACCAAG
    GTGCTTCTTC ATTCCATGGT TCGGGACGGG CAGGGCCGGA AGATGAGCAA GTCCCTGGGG
    AATGTATTGG ACCCACGAGA CATCATCAGT GGGGTGGAAC TGCAGGTGCT GCAGGAAAAG
    CTGAAGGACG GGAACTTGGA CTCCACAGAG CTGGCGATTG CGGCTGCGGC ACAGCTGTGT
    AGGCATCTGC CACCCTTCTT ATCCCTCTGG TTGCAGAGGA AGGACTTCCC TCATGGGATC
    CCTGAGTGTG GGACAGATGC CCTGCGATTT GCCCTGTGCT CCCACAGGGC CTTGGGGGGC
    GATGTACACT TGTCCGTCTC TGAGGTCTTG AGTTCCCGGC ATTTCTGCAA CAAAATCTGG
    AATGCCCTGC GTTTTATCCT CAATGCCTTA GGAGAGGGAT TTGTACCGCA GCCTGCAGAG
    GAGCTATCCC CCTCATCCCC CACGGACGCC TGGATCCTGA GCCGTCTGGC CCACACTGCC
    CAGGAGTGTG AGCGAGCCTT CCTTGCTCGG GAGCTGCCAC TCATCACCCA TGCCCTCCAC
    CACTTCTGGC TCCACAGCCT CTGTGATGTC TACTTGGAAG CTGTGAAGCC AGTGCTTTCG
    CACTCGCCCC ATCCCCAAGG CCCGCCTCAG GTCCTGTTCT CCTGCGCTGA TGTGGGTCTC
    CGCCTCCTCG CCCCACTGAT GCCCTTCCTG GCTGAAGAGC TCTGGCAGAG GCTGCCACCC
    AGGCCTGGCA GCAACTCTGC CCCTAGCATC TCTGTTGCTC CCTACCCCAC CACCCATAGC
    TTGGAGCACT GGCGCCAGCC TGCGCTGGAA CAACACTTCT CTCAGGTCCA GGAGGCTGTG
    CAGGTACTGA GGGCCCTCCG AGCCACGTAC CAGCTCACCA AGGCCCGGCC CCGAGTACTG
    CTGCAGAGCT CAGAGCCTGG AGAGCTGAGC CTCTTCAAGC CCTTCCTGGA GCCCTTGGCC
    ACACTGGGCC ACTGTGGGGC CGTGGGCCTG TTACCCCCAG GTGTAGCGGC ACCCTCCGGC
    TGGGCTCAGG CCCCACTCAG TGACACCATT CAGGTGTACA TGGAGCTACA GGGCCTGGTG
    GACCCCCAGG CCCATCTGGC TCTGCTAGCT GCCCGAAGAC GCAAGTTGCA GAAGCAGCTG
    GATGGCCTCA TGGCCTGGAC CCCATCAGGG GGAGAGGAAG AGACTAAGAG GCAGCAGAGG
    CTTTCTTCCC TCCAGCTGGA GTTGTCAAAG CTGGACAAGG CAGCCTCTCA CCTCCGGCAG
    CTGACGGATG CATGTCCCAG CCCCAAGGAG CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe39500
    Clone ID Related Accession (Same CDS sequence) XM_014118302.1 , XM_014118302.2
    Accession Version XM_014118302.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3234bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product valine--tRNA ligase, mitochondrial isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726081.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGCTGCGGG TCTGGAGCGC CTCTCCGCCT TTGCAGACAC TCCTGATGCC TCGTTCGCCC 
    TTGGCCTCGC TTCCATCACG GTGCTGGGGG CTCGGGCCCT CTCAGGCCCT CCGCAGGTCC
    CACCCTCTCT CTACCCAGTC AGAGCCGTGC CGGTCCTCCC TCTCCCGGAG GAACCATGAA
    GCCAAACAGA AGCGCCAGCG GGAGAAGCAG ATGGCGCTGG AGGCTGGGAT CACCCCGAAG
    GGCAAGGCAC CTGCAGGATC CAGTAAGGCC TGGACTCCTA AGGAGGTAGT GTTGTATGAA
    GTCCCCACGG AACCGGGTGA AAAGAAAGAT GTGTACCGCC TCCTGCCTCC TGCATACAGT
    CCTCGCTACG TTGAGGCGGC CTGGTACTCC TGGTGGGTGC GAGAGGGCTT CTTCAAACCG
    GAGTATCAGA CCAGGCTGCC CCAGGCCACA GGGGAGACGT TTTCCATGTG TATCCCACCT
    CCCAATGTCA CTGGCTCCCT GCATATCGGT CATGCACTGA CAGTAGCCAT ACAGGATGCC
    CTGGTGCGCT GGCACCGGAT GCGTGGGGAT CAGGTGCTAT GGATCCCTGG CTCAGATCAT
    GCAGGAATTG CTACACAAGC TGTGGTGGAG AAGCAACTGT GGAAGGAGCG AGGAATGAGG
    AGACATGAAC TGAGCCGGGA AAAATTCCTT AGGGAGGTGT GGAAGTGGAA GGACGAGAAA
    GGTGGAGAGA TCAGTGAGCA GCTCCAAGCT CTGGGGGCCT CCCTGGACTG GGACCGAGAG
    TGCTTCACTA TGGATGCTGG CTTCTCAGTG GCCGTGACCG AAGCTTTTGT GCGACTCTAC
    GAGGCAGGAT TGTTGTACCG GAGCCAGCAG CTTGTCAACT GGTCCTGCAC TTTAAGATCA
    GCCATCTCGG ATATTGAGGT GGAGAGCCGG CCCCTGCCTG GCCACACGGA GCTTCAACTG
    CCTGGCTGCC CCACTCCTGT GTCTTTTGGC CTCCTTGTGT CTGTGGCCTT CCCTGTGGAT
    GGAGAGCCTG ATGCAGAGGT CGTGGTAGGA ACTACAAGGC CAGAGACGTT GCCTGGAGAC
    GTTGCTGTGG CTGTTCATCC CGACGACTCC CGATACACAC ACCTGCATGG GCGACAGCTT
    CGTCACCCCT TGACTGGGCA GCTTCTCCCC CTCATCACAG ACTCCACTGT TCAGCCACAT
    GTGGGCACAG GGGCAGTGAA GGTGACTCCA GCGCATAGCC CTGCTGATGC TGAGCTGGGG
    GCCCGACACG GCTTGAGCCC TCTGAGTGTC ATCGCGGAGG ATGGCACCAT GACCTCTCTC
    TGTGGGGACT GGCTACAGGG TCTTCACCGA TTTGTGGCCC GGGAAAAGAT TATATCTGCG
    CTGCGGGAGC GGGGCCTGTT CCGGGGCCTC CAGAACCACC CCATGGTGCT TCCTATTTGC
    AGCCGTTCTG GGGACGTGAT AGAATATCTA CTGAAGAGCC AGTGGTTTGT CCGCTGCCGG
    GAGATGGGGG ACCGAGCTGC CCAGGCCGTG GAGTCAGGGG CTCTGGAGCT CAGTCCATCC
    TTCCACCAGA AGAACTGGCA GCACTGGTTT TCCCACATCG GGGACTGGTG TGTCTCCCGG
    CAGCTGTGGT GGGGCCATCG GATTCCCGCC TACCTGGTTG TAGAGGAGCA CATGAAGGGT
    GATGGAGAAG ACTGTTGGGT GGTTGGGCGG ACAGAGACCG AGGCCAGAAA AATAGCTGCA
    GAACTGACTG GGAGATCAGA GGCAGAGCTG ACCCTGCAGA GGGATCCTGA TGTCCTGGAC
    ACATGGTTCT CTTCGGCTCT TTTCCCCTTT GCTGCCTTGG GCTGGCCCCA AGAGACCCCA
    GACCTTGCTC GTTTCTACCC CCTGTCACTT TTGGAAACAG GCAGTGATCT CCTGTTGTTC
    TGGGTGGGCC GCATGGTCAT GTTGGGGACA CAGCTCACAG GGCAGCTCCC CTTCACCAAG
    GTGCTTCTTC ATTCCATGGT TCGGGACGGG CAGGGCCGGA AGATGAGCAA GTCCCTGGGG
    AATGTATTGG ACCCACGAGA CATCATCAGT GGGGTGGAAC TGCAGGTGCT GCAGGAAAAG
    CTGAAGGACG GGAACTTGGA CTCCACAGAG CTGGCGATTG CGGCTGCGGC ACAGAGGAAG
    GACTTCCCTC ATGGGATCCC TGAGTGTGGG ACAGATGCCC TGCGATTTGC CCTGTGCTCC
    CACAGGGCCT TGGGGGGCGA TGTACACTTG TCCGTCTCTG AGGTCTTGAG TTCCCGGCAT
    TTCTGCAACA AAATCTGGAA TGCCCTGCGT TTTATCCTCA ATGCCTTAGG AGAGGGATTT
    GTACCGCAGC CTGCAGAGGA GCTATCCCCC TCATCCCCCA CGGACGCCTG GATCCTGAGC
    CGTCTGGCCC ACACTGCCCA GGAGTGTGAG CGAGCCTTCC TTGCTCGGGA GCTGCCACTC
    ATCACCCATG CCCTCCACCA CTTCTGGCTC CACAGCCTCT GTGATGTCTA CTTGGAAGCT
    GTGAAGCCAG TGCTTTCGCA CTCGCCCCAT CCCCAAGGCC CGCCTCAGGT CCTGTTCTCC
    TGCGCTGATG TGGGTCTCCG CCTCCTCGCC CCACTGATGC CCTTCCTGGC TGAAGAGCTC
    TGGCAGAGGC TGCCACCCAG GCCTGGCAGC AACTCTGCCC CTAGCATCTC TGTTGCTCCC
    TACCCCACCA CCCATAGCTT GGAGCACTGG CGCCAGCCTG CGCTGGAACA ACACTTCTCT
    CAGGTCCAGG AGGCTGTGCA GGTACTGAGG GCCCTCCGAG CCACGTACCA GCTCACCAAG
    GCCCGGCCCC GAGTACTGCT GCAGAGCTCA GAGCCTGGAG AGCTGAGCCT CTTCAAGCCC
    TTCCTGGAGC CCTTGGCCAC ACTGGGCCAC TGTGGGGCCG TGGGCCTGTT ACCCCCAGGT
    GTAGCGGCAC CCTCCGGCTG GGCTCAGGCC CCACTCAGTG ACACCATTCA GGTGTACATG
    GAGCTACAGG GCCTGGTGGA CCCCCAGGCC CATCTGGCTC TGCTAGCTGC CCGAAGACGC
    AAGTTGCAGA AGCAGCTGGA TGGCCTCATG GCCTGGACCC CATCAGGGGG AGAGGAAGAG
    ACTAAGAGGC AGCAGAGGCT TTCTTCCCTC CAGCTGGAGT TGTCAAAGCT GGACAAGGCA
    GCCTCTCACC TCCGGCAGCT GACGGATGCA TGTCCCAGCC CCAAGGAGCT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013973777.1
    CDS131..3364
    Translation

    Target ORF information:

    RefSeq Version XM_014118302.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014118302.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGCTGCGGG TCTGGAGCGC CTCTCCGCCT TTGCAGACAC TCCTGATGCC TCGTTCGCCC 
    TTGGCCTCGC TTCCATCACG GTGCTGGGGG CTCGGGCCCT CTCAGGCCCT CCGCAGGTCC
    CACCCTCTCT CTACCCAGTC AGAGCCGTGC CGGTCCTCCC TCTCCCGGAG GAACCATGAA
    GCCAAACAGA AGCGCCAGCG GGAGAAGCAG ATGGCGCTGG AGGCTGGGAT CACCCCGAAG
    GGCAAGGCAC CTGCAGGATC CAGTAAGGCC TGGACTCCTA AGGAGGTAGT GTTGTATGAA
    GTCCCCACGG AACCGGGTGA AAAGAAAGAT GTGTACCGCC TCCTGCCTCC TGCATACAGT
    CCTCGCTACG TTGAGGCGGC CTGGTACTCC TGGTGGGTGC GAGAGGGCTT CTTCAAACCG
    GAGTATCAGA CCAGGCTGCC CCAGGCCACA GGGGAGACGT TTTCCATGTG TATCCCACCT
    CCCAATGTCA CTGGCTCCCT GCATATCGGT CATGCACTGA CAGTAGCCAT ACAGGATGCC
    CTGGTGCGCT GGCACCGGAT GCGTGGGGAT CAGGTGCTAT GGATCCCTGG CTCAGATCAT
    GCAGGAATTG CTACACAAGC TGTGGTGGAG AAGCAACTGT GGAAGGAGCG AGGAATGAGG
    AGACATGAAC TGAGCCGGGA AAAATTCCTT AGGGAGGTGT GGAAGTGGAA GGACGAGAAA
    GGTGGAGAGA TCAGTGAGCA GCTCCAAGCT CTGGGGGCCT CCCTGGACTG GGACCGAGAG
    TGCTTCACTA TGGATGCTGG CTTCTCAGTG GCCGTGACCG AAGCTTTTGT GCGACTCTAC
    GAGGCAGGAT TGTTGTACCG GAGCCAGCAG CTTGTCAACT GGTCCTGCAC TTTAAGATCA
    GCCATCTCGG ATATTGAGGT GGAGAGCCGG CCCCTGCCTG GCCACACGGA GCTTCAACTG
    CCTGGCTGCC CCACTCCTGT GTCTTTTGGC CTCCTTGTGT CTGTGGCCTT CCCTGTGGAT
    GGAGAGCCTG ATGCAGAGGT CGTGGTAGGA ACTACAAGGC CAGAGACGTT GCCTGGAGAC
    GTTGCTGTGG CTGTTCATCC CGACGACTCC CGATACACAC ACCTGCATGG GCGACAGCTT
    CGTCACCCCT TGACTGGGCA GCTTCTCCCC CTCATCACAG ACTCCACTGT TCAGCCACAT
    GTGGGCACAG GGGCAGTGAA GGTGACTCCA GCGCATAGCC CTGCTGATGC TGAGCTGGGG
    GCCCGACACG GCTTGAGCCC TCTGAGTGTC ATCGCGGAGG ATGGCACCAT GACCTCTCTC
    TGTGGGGACT GGCTACAGGG TCTTCACCGA TTTGTGGCCC GGGAAAAGAT TATATCTGCG
    CTGCGGGAGC GGGGCCTGTT CCGGGGCCTC CAGAACCACC CCATGGTGCT TCCTATTTGC
    AGCCGTTCTG GGGACGTGAT AGAATATCTA CTGAAGAGCC AGTGGTTTGT CCGCTGCCGG
    GAGATGGGGG ACCGAGCTGC CCAGGCCGTG GAGTCAGGGG CTCTGGAGCT CAGTCCATCC
    TTCCACCAGA AGAACTGGCA GCACTGGTTT TCCCACATCG GGGACTGGTG TGTCTCCCGG
    CAGCTGTGGT GGGGCCATCG GATTCCCGCC TACCTGGTTG TAGAGGAGCA CATGAAGGGT
    GATGGAGAAG ACTGTTGGGT GGTTGGGCGG ACAGAGACCG AGGCCAGAAA AATAGCTGCA
    GAACTGACTG GGAGATCAGA GGCAGAGCTG ACCCTGCAGA GGGATCCTGA TGTCCTGGAC
    ACATGGTTCT CTTCGGCTCT TTTCCCCTTT GCTGCCTTGG GCTGGCCCCA AGAGACCCCA
    GACCTTGCTC GTTTCTACCC CCTGTCACTT TTGGAAACAG GCAGTGATCT CCTGTTGTTC
    TGGGTGGGCC GCATGGTCAT GTTGGGGACA CAGCTCACAG GGCAGCTCCC CTTCACCAAG
    GTGCTTCTTC ATTCCATGGT TCGGGACGGG CAGGGCCGGA AGATGAGCAA GTCCCTGGGG
    AATGTATTGG ACCCACGAGA CATCATCAGT GGGGTGGAAC TGCAGGTGCT GCAGGAAAAG
    CTGAAGGACG GGAACTTGGA CTCCACAGAG CTGGCGATTG CGGCTGCGGC ACAGAGGAAG
    GACTTCCCTC ATGGGATCCC TGAGTGTGGG ACAGATGCCC TGCGATTTGC CCTGTGCTCC
    CACAGGGCCT TGGGGGGCGA TGTACACTTG TCCGTCTCTG AGGTCTTGAG TTCCCGGCAT
    TTCTGCAACA AAATCTGGAA TGCCCTGCGT TTTATCCTCA ATGCCTTAGG AGAGGGATTT
    GTACCGCAGC CTGCAGAGGA GCTATCCCCC TCATCCCCCA CGGACGCCTG GATCCTGAGC
    CGTCTGGCCC ACACTGCCCA GGAGTGTGAG CGAGCCTTCC TTGCTCGGGA GCTGCCACTC
    ATCACCCATG CCCTCCACCA CTTCTGGCTC CACAGCCTCT GTGATGTCTA CTTGGAAGCT
    GTGAAGCCAG TGCTTTCGCA CTCGCCCCAT CCCCAAGGCC CGCCTCAGGT CCTGTTCTCC
    TGCGCTGATG TGGGTCTCCG CCTCCTCGCC CCACTGATGC CCTTCCTGGC TGAAGAGCTC
    TGGCAGAGGC TGCCACCCAG GCCTGGCAGC AACTCTGCCC CTAGCATCTC TGTTGCTCCC
    TACCCCACCA CCCATAGCTT GGAGCACTGG CGCCAGCCTG CGCTGGAACA ACACTTCTCT
    CAGGTCCAGG AGGCTGTGCA GGTACTGAGG GCCCTCCGAG CCACGTACCA GCTCACCAAG
    GCCCGGCCCC GAGTACTGCT GCAGAGCTCA GAGCCTGGAG AGCTGAGCCT CTTCAAGCCC
    TTCCTGGAGC CCTTGGCCAC ACTGGGCCAC TGTGGGGCCG TGGGCCTGTT ACCCCCAGGT
    GTAGCGGCAC CCTCCGGCTG GGCTCAGGCC CCACTCAGTG ACACCATTCA GGTGTACATG
    GAGCTACAGG GCCTGGTGGA CCCCCAGGCC CATCTGGCTC TGCTAGCTGC CCGAAGACGC
    AAGTTGCAGA AGCAGCTGGA TGGCCTCATG GCCTGGACCC CATCAGGGGG AGAGGAAGAG
    ACTAAGAGGC AGCAGAGGCT TTCTTCCCTC CAGCTGGAGT TGTCAAAGCT GGACAAGGCA
    GCCTCTCACC TCCGGCAGCT GACGGATGCA TGTCCCAGCC CCAAGGAGCT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe39500
    Clone ID Related Accession (Same CDS sequence) XM_014118302.1 , XM_014118302.2
    Accession Version XM_014118302.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3234bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product valine--tRNA ligase, mitochondrial isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006594.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014118302.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGCTGCGGG TCTGGAGCGC CTCTCCGCCT TTGCAGACAC TCCTGATGCC TCGTTCGCCC 
    TTGGCCTCGC TTCCATCACG GTGCTGGGGG CTCGGGCCCT CTCAGGCCCT CCGCAGGTCC
    CACCCTCTCT CTACCCAGTC AGAGCCGTGC CGGTCCTCCC TCTCCCGGAG GAACCATGAA
    GCCAAACAGA AGCGCCAGCG GGAGAAGCAG ATGGCGCTGG AGGCTGGGAT CACCCCGAAG
    GGCAAGGCAC CTGCAGGATC CAGTAAGGCC TGGACTCCTA AGGAGGTAGT GTTGTATGAA
    GTCCCCACGG AACCGGGTGA AAAGAAAGAT GTGTACCGCC TCCTGCCTCC TGCATACAGT
    CCTCGCTACG TTGAGGCGGC CTGGTACTCC TGGTGGGTGC GAGAGGGCTT CTTCAAACCG
    GAGTATCAGA CCAGGCTGCC CCAGGCCACA GGGGAGACGT TTTCCATGTG TATCCCACCT
    CCCAATGTCA CTGGCTCCCT GCATATCGGT CATGCACTGA CAGTAGCCAT ACAGGATGCC
    CTGGTGCGCT GGCACCGGAT GCGTGGGGAT CAGGTGCTAT GGATCCCTGG CTCAGATCAT
    GCAGGAATTG CTACACAAGC TGTGGTGGAG AAGCAACTGT GGAAGGAGCG AGGAATGAGG
    AGACATGAAC TGAGCCGGGA AAAATTCCTT AGGGAGGTGT GGAAGTGGAA GGACGAGAAA
    GGTGGAGAGA TCAGTGAGCA GCTCCAAGCT CTGGGGGCCT CCCTGGACTG GGACCGAGAG
    TGCTTCACTA TGGATGCTGG CTTCTCAGTG GCCGTGACCG AAGCTTTTGT GCGACTCTAC
    GAGGCAGGAT TGTTGTACCG GAGCCAGCAG CTTGTCAACT GGTCCTGCAC TTTAAGATCA
    GCCATCTCGG ATATTGAGGT GGAGAGCCGG CCCCTGCCTG GCCACACGGA GCTTCAACTG
    CCTGGCTGCC CCACTCCTGT GTCTTTTGGC CTCCTTGTGT CTGTGGCCTT CCCTGTGGAT
    GGAGAGCCTG ATGCAGAGGT CGTGGTAGGA ACTACAAGGC CAGAGACGTT GCCTGGAGAC
    GTTGCTGTGG CTGTTCATCC CGACGACTCC CGATACACAC ACCTGCATGG GCGACAGCTT
    CGTCACCCCT TGACTGGGCA GCTTCTCCCC CTCATCACAG ACTCCACTGT TCAGCCACAT
    GTGGGCACAG GGGCAGTGAA GGTGACTCCA GCGCATAGCC CTGCTGATGC TGAGCTGGGG
    GCCCGACACG GCTTGAGCCC TCTGAGTGTC ATCGCGGAGG ATGGCACCAT GACCTCTCTC
    TGTGGGGACT GGCTACAGGG TCTTCACCGA TTTGTGGCCC GGGAAAAGAT TATATCTGCG
    CTGCGGGAGC GGGGCCTGTT CCGGGGCCTC CAGAACCACC CCATGGTGCT TCCTATTTGC
    AGCCGTTCTG GGGACGTGAT AGAATATCTA CTGAAGAGCC AGTGGTTTGT CCGCTGCCGG
    GAGATGGGGG ACCGAGCTGC CCAGGCCGTG GAGTCAGGGG CTCTGGAGCT CAGTCCATCC
    TTCCACCAGA AGAACTGGCA GCACTGGTTT TCCCACATCG GGGACTGGTG TGTCTCCCGG
    CAGCTGTGGT GGGGCCATCG GATTCCCGCC TACCTGGTTG TAGAGGAGCA CATGAAGGGT
    GATGGAGAAG ACTGTTGGGT GGTTGGGCGG ACAGAGACCG AGGCCAGAAA AATAGCTGCA
    GAACTGACTG GGAGATCAGA GGCAGAGCTG ACCCTGCAGA GGGATCCTGA TGTCCTGGAC
    ACATGGTTCT CTTCGGCTCT TTTCCCCTTT GCTGCCTTGG GCTGGCCCCA AGAGACCCCA
    GACCTTGCTC GTTTCTACCC CCTGTCACTT TTGGAAACAG GCAGTGATCT CCTGTTGTTC
    TGGGTGGGCC GCATGGTCAT GTTGGGGACA CAGCTCACAG GGCAGCTCCC CTTCACCAAG
    GTGCTTCTTC ATTCCATGGT TCGGGACGGG CAGGGCCGGA AGATGAGCAA GTCCCTGGGG
    AATGTATTGG ACCCACGAGA CATCATCAGT GGGGTGGAAC TGCAGGTGCT GCAGGAAAAG
    CTGAAGGACG GGAACTTGGA CTCCACAGAG CTGGCGATTG CGGCTGCGGC ACAGAGGAAG
    GACTTCCCTC ATGGGATCCC TGAGTGTGGG ACAGATGCCC TGCGATTTGC CCTGTGCTCC
    CACAGGGCCT TGGGGGGCGA TGTACACTTG TCCGTCTCTG AGGTCTTGAG TTCCCGGCAT
    TTCTGCAACA AAATCTGGAA TGCCCTGCGT TTTATCCTCA ATGCCTTAGG AGAGGGATTT
    GTACCGCAGC CTGCAGAGGA GCTATCCCCC TCATCCCCCA CGGACGCCTG GATCCTGAGC
    CGTCTGGCCC ACACTGCCCA GGAGTGTGAG CGAGCCTTCC TTGCTCGGGA GCTGCCACTC
    ATCACCCATG CCCTCCACCA CTTCTGGCTC CACAGCCTCT GTGATGTCTA CTTGGAAGCT
    GTGAAGCCAG TGCTTTCGCA CTCGCCCCAT CCCCAAGGCC CGCCTCAGGT CCTGTTCTCC
    TGCGCTGATG TGGGTCTCCG CCTCCTCGCC CCACTGATGC CCTTCCTGGC TGAAGAGCTC
    TGGCAGAGGC TGCCACCCAG GCCTGGCAGC AACTCTGCCC CTAGCATCTC TGTTGCTCCC
    TACCCCACCA CCCATAGCTT GGAGCACTGG CGCCAGCCTG CGCTGGAACA ACACTTCTCT
    CAGGTCCAGG AGGCTGTGCA GGTACTGAGG GCCCTCCGAG CCACGTACCA GCTCACCAAG
    GCCCGGCCCC GAGTACTGCT GCAGAGCTCA GAGCCTGGAG AGCTGAGCCT CTTCAAGCCC
    TTCCTGGAGC CCTTGGCCAC ACTGGGCCAC TGTGGGGCCG TGGGCCTGTT ACCCCCAGGT
    GTAGCGGCAC CCTCCGGCTG GGCTCAGGCC CCACTCAGTG ACACCATTCA GGTGTACATG
    GAGCTACAGG GCCTGGTGGA CCCCCAGGCC CATCTGGCTC TGCTAGCTGC CCGAAGACGC
    AAGTTGCAGA AGCAGCTGGA TGGCCTCATG GCCTGGACCC CATCAGGGGG AGAGGAAGAG
    ACTAAGAGGC AGCAGAGGCT TTCTTCCCTC CAGCTGGAGT TGTCAAAGCT GGACAAGGCA
    GCCTCTCACC TCCGGCAGCT GACGGATGCA TGTCCCAGCC CCAAGGAGCT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013973777.1
    CDS100..3333
    Translation

    Target ORF information:

    RefSeq Version XM_014118302.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014118302.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGCTGCGGG TCTGGAGCGC CTCTCCGCCT TTGCAGACAC TCCTGATGCC TCGTTCGCCC 
    TTGGCCTCGC TTCCATCACG GTGCTGGGGG CTCGGGCCCT CTCAGGCCCT CCGCAGGTCC
    CACCCTCTCT CTACCCAGTC AGAGCCGTGC CGGTCCTCCC TCTCCCGGAG GAACCATGAA
    GCCAAACAGA AGCGCCAGCG GGAGAAGCAG ATGGCGCTGG AGGCTGGGAT CACCCCGAAG
    GGCAAGGCAC CTGCAGGATC CAGTAAGGCC TGGACTCCTA AGGAGGTAGT GTTGTATGAA
    GTCCCCACGG AACCGGGTGA AAAGAAAGAT GTGTACCGCC TCCTGCCTCC TGCATACAGT
    CCTCGCTACG TTGAGGCGGC CTGGTACTCC TGGTGGGTGC GAGAGGGCTT CTTCAAACCG
    GAGTATCAGA CCAGGCTGCC CCAGGCCACA GGGGAGACGT TTTCCATGTG TATCCCACCT
    CCCAATGTCA CTGGCTCCCT GCATATCGGT CATGCACTGA CAGTAGCCAT ACAGGATGCC
    CTGGTGCGCT GGCACCGGAT GCGTGGGGAT CAGGTGCTAT GGATCCCTGG CTCAGATCAT
    GCAGGAATTG CTACACAAGC TGTGGTGGAG AAGCAACTGT GGAAGGAGCG AGGAATGAGG
    AGACATGAAC TGAGCCGGGA AAAATTCCTT AGGGAGGTGT GGAAGTGGAA GGACGAGAAA
    GGTGGAGAGA TCAGTGAGCA GCTCCAAGCT CTGGGGGCCT CCCTGGACTG GGACCGAGAG
    TGCTTCACTA TGGATGCTGG CTTCTCAGTG GCCGTGACCG AAGCTTTTGT GCGACTCTAC
    GAGGCAGGAT TGTTGTACCG GAGCCAGCAG CTTGTCAACT GGTCCTGCAC TTTAAGATCA
    GCCATCTCGG ATATTGAGGT GGAGAGCCGG CCCCTGCCTG GCCACACGGA GCTTCAACTG
    CCTGGCTGCC CCACTCCTGT GTCTTTTGGC CTCCTTGTGT CTGTGGCCTT CCCTGTGGAT
    GGAGAGCCTG ATGCAGAGGT CGTGGTAGGA ACTACAAGGC CAGAGACGTT GCCTGGAGAC
    GTTGCTGTGG CTGTTCATCC CGACGACTCC CGATACACAC ACCTGCATGG GCGACAGCTT
    CGTCACCCCT TGACTGGGCA GCTTCTCCCC CTCATCACAG ACTCCACTGT TCAGCCACAT
    GTGGGCACAG GGGCAGTGAA GGTGACTCCA GCGCATAGCC CTGCTGATGC TGAGCTGGGG
    GCCCGACACG GCTTGAGCCC TCTGAGTGTC ATCGCGGAGG ATGGCACCAT GACCTCTCTC
    TGTGGGGACT GGCTACAGGG TCTTCACCGA TTTGTGGCCC GGGAAAAGAT TATATCTGCG
    CTGCGGGAGC GGGGCCTGTT CCGGGGCCTC CAGAACCACC CCATGGTGCT TCCTATTTGC
    AGCCGTTCTG GGGACGTGAT AGAATATCTA CTGAAGAGCC AGTGGTTTGT CCGCTGCCGG
    GAGATGGGGG ACCGAGCTGC CCAGGCCGTG GAGTCAGGGG CTCTGGAGCT CAGTCCATCC
    TTCCACCAGA AGAACTGGCA GCACTGGTTT TCCCACATCG GGGACTGGTG TGTCTCCCGG
    CAGCTGTGGT GGGGCCATCG GATTCCCGCC TACCTGGTTG TAGAGGAGCA CATGAAGGGT
    GATGGAGAAG ACTGTTGGGT GGTTGGGCGG ACAGAGACCG AGGCCAGAAA AATAGCTGCA
    GAACTGACTG GGAGATCAGA GGCAGAGCTG ACCCTGCAGA GGGATCCTGA TGTCCTGGAC
    ACATGGTTCT CTTCGGCTCT TTTCCCCTTT GCTGCCTTGG GCTGGCCCCA AGAGACCCCA
    GACCTTGCTC GTTTCTACCC CCTGTCACTT TTGGAAACAG GCAGTGATCT CCTGTTGTTC
    TGGGTGGGCC GCATGGTCAT GTTGGGGACA CAGCTCACAG GGCAGCTCCC CTTCACCAAG
    GTGCTTCTTC ATTCCATGGT TCGGGACGGG CAGGGCCGGA AGATGAGCAA GTCCCTGGGG
    AATGTATTGG ACCCACGAGA CATCATCAGT GGGGTGGAAC TGCAGGTGCT GCAGGAAAAG
    CTGAAGGACG GGAACTTGGA CTCCACAGAG CTGGCGATTG CGGCTGCGGC ACAGAGGAAG
    GACTTCCCTC ATGGGATCCC TGAGTGTGGG ACAGATGCCC TGCGATTTGC CCTGTGCTCC
    CACAGGGCCT TGGGGGGCGA TGTACACTTG TCCGTCTCTG AGGTCTTGAG TTCCCGGCAT
    TTCTGCAACA AAATCTGGAA TGCCCTGCGT TTTATCCTCA ATGCCTTAGG AGAGGGATTT
    GTACCGCAGC CTGCAGAGGA GCTATCCCCC TCATCCCCCA CGGACGCCTG GATCCTGAGC
    CGTCTGGCCC ACACTGCCCA GGAGTGTGAG CGAGCCTTCC TTGCTCGGGA GCTGCCACTC
    ATCACCCATG CCCTCCACCA CTTCTGGCTC CACAGCCTCT GTGATGTCTA CTTGGAAGCT
    GTGAAGCCAG TGCTTTCGCA CTCGCCCCAT CCCCAAGGCC CGCCTCAGGT CCTGTTCTCC
    TGCGCTGATG TGGGTCTCCG CCTCCTCGCC CCACTGATGC CCTTCCTGGC TGAAGAGCTC
    TGGCAGAGGC TGCCACCCAG GCCTGGCAGC AACTCTGCCC CTAGCATCTC TGTTGCTCCC
    TACCCCACCA CCCATAGCTT GGAGCACTGG CGCCAGCCTG CGCTGGAACA ACACTTCTCT
    CAGGTCCAGG AGGCTGTGCA GGTACTGAGG GCCCTCCGAG CCACGTACCA GCTCACCAAG
    GCCCGGCCCC GAGTACTGCT GCAGAGCTCA GAGCCTGGAG AGCTGAGCCT CTTCAAGCCC
    TTCCTGGAGC CCTTGGCCAC ACTGGGCCAC TGTGGGGCCG TGGGCCTGTT ACCCCCAGGT
    GTAGCGGCAC CCTCCGGCTG GGCTCAGGCC CCACTCAGTG ACACCATTCA GGTGTACATG
    GAGCTACAGG GCCTGGTGGA CCCCCAGGCC CATCTGGCTC TGCTAGCTGC CCGAAGACGC
    AAGTTGCAGA AGCAGCTGGA TGGCCTCATG GCCTGGACCC CATCAGGGGG AGAGGAAGAG
    ACTAAGAGGC AGCAGAGGCT TTCTTCCCTC CAGCTGGAGT TGTCAAAGCT GGACAAGGCA
    GCCTCTCACC TCCGGCAGCT GACGGATGCA TGTCCCAGCC CCAAGGAGCT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe14991
    Clone ID Related Accession (Same CDS sequence) XM_005627121.2 , XM_005627121.3
    Accession Version XM_005627121.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3279bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product valine--tRNA ligase, mitochondrial isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726081.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005627121.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCCTCGTT CGCCCTTGGC CTCGCTTCCA TCACGGTGCT GGGGGCTCGG GCCCTCTCAG 
    GCCCTCCGCA GGTCCCACCC TCTCTCTACC CAGTCAGAGC CGTGCCGGTC CTCCCTCTCC
    CGGAGGAACC ATGAAGCCAA ACAGAAGCGC CAGCGGGAGA AGCAGATGGC GCTGGAGGCT
    GGGATCACCC CGAAGGGCAA GGCACCTGCA GGATCCAGTA AGGCCTGGAC TCCTAAGGAG
    GTAGTGTTGT ATGAAGTCCC CACGGAACCG GGTGAAAAGA AAGATGTGTA CCGCCTCCTG
    CCTCCTGCAT ACAGTCCTCG CTACGTTGAG GCGGCCTGGT ACTCCTGGTG GGTGCGAGAG
    GGCTTCTTCA AACCGGAGTA TCAGACCAGG CTGCCCCAGG CCACAGGGGA GACGTTTTCC
    ATGTGTATCC CACCTCCCAA TGTCACTGGC TCCCTGCATA TCGGTCATGC ACTGACAGTA
    GCCATACAGG ATGCCCTGGT GCGCTGGCAC CGGATGCGTG GGGATCAGGT GCTATGGATC
    CCTGGCTCAG ATCATGCAGG AATTGCTACA CAAGCTGTGG TGGAGAAGCA ACTGTGGAAG
    GAGCGAGGAA TGAGGAGACA TGAACTGAGC CGGGAAAAAT TCCTTAGGGA GGTGTGGAAG
    TGGAAGGACG AGAAAGGTGG AGAGATCAGT GAGCAGCTCC AAGCTCTGGG GGCCTCCCTG
    GACTGGGACC GAGAGTGCTT CACTATGGAT GCTGGCTTCT CAGTGGCCGT GACCGAAGCT
    TTTGTGCGAC TCTACGAGGC AGGATTGTTG TACCGGAGCC AGCAGCTTGT CAACTGGTCC
    TGCACTTTAA GATCAGCCAT CTCGGATATT GAGGTGGAGA GCCGGCCCCT GCCTGGCCAC
    ACGGAGCTTC AACTGCCTGG CTGCCCCACT CCTGTGTCTT TTGGCCTCCT TGTGTCTGTG
    GCCTTCCCTG TGGATGGAGA GCCTGATGCA GAGGTCGTGG TAGGAACTAC AAGGCCAGAG
    ACGTTGCCTG GAGACGTTGC TGTGGCTGTT CATCCCGACG ACTCCCGATA CACACACCTG
    CATGGGCGAC AGCTTCGTCA CCCCTTGACT GGGCAGCTTC TCCCCCTCAT CACAGACTCC
    ACTGTTCAGC CACATGTGGG CACAGGGGCA GTGAAGGTGA CTCCAGCGCA TAGCCCTGCT
    GATGCTGAGC TGGGGGCCCG ACACGGCTTG AGCCCTCTGA GTGTCATCGC GGAGGATGGC
    ACCATGACCT CTCTCTGTGG GGACTGGCTA CAGGGTCTTC ACCGATTTGT GGCCCGGGAA
    AAGATTATAT CTGCGCTGCG GGAGCGGGGC CTGTTCCGGG GCCTCCAGAA CCACCCCATG
    GTGCTTCCTA TTTGCAGCCG TTCTGGGGAC GTGATAGAAT ATCTACTGAA GAGCCAGTGG
    TTTGTCCGCT GCCGGGAGAT GGGGGACCGA GCTGCCCAGA TGCAAGTGAG GTGCAGGAAG
    GGAAGCTCTA ACGGCCTTTC TGTTCAGGCC GTGGAGTCAG GGGCTCTGGA GCTCAGTCCA
    TCCTTCCACC AGAAGAACTG GCAGCACTGG TTTTCCCACA TCGGGGACTG GTGTGTCTCC
    CGGCAGCTGT GGTGGGGCCA TCGGATTCCC GCCTACCTGG TTGTAGAGGA GCACATGAAG
    GGTGATGGAG AAGACTGTTG GGTGGTTGGG CGGACAGAGA CCGAGGCCAG AAAAATAGCT
    GCAGAACTGA CTGGGAGATC AGAGGCAGAG CTGACCCTGC AGAGGGATCC TGATGTCCTG
    GACACATGGT TCTCTTCGGC TCTTTTCCCC TTTGCTGCCT TGGGCTGGCC CCAAGAGACC
    CCAGACCTTG CTCGTTTCTA CCCCCTGTCA CTTTTGGAAA CAGGCAGTGA TCTCCTGTTG
    TTCTGGGTGG GCCGCATGGT CATGTTGGGG ACACAGCTCA CAGGGCAGCT CCCCTTCACC
    AAGGTGCTTC TTCATTCCAT GGTTCGGGAC GGGCAGGGCC GGAAGATGAG CAAGTCCCTG
    GGGAATGTAT TGGACCCACG AGACATCATC AGTGGGGTGG AACTGCAGGT GCTGCAGGAA
    AAGCTGAAGG ACGGGAACTT GGACTCCACA GAGCTGGCGA TTGCGGCTGC GGCACAGCTG
    TGTAGGCATC TGCCACCCTT CTTATCCCTC TGGTTGCAGA GGAAGGACTT CCCTCATGGG
    ATCCCTGAGT GTGGGACAGA TGCCCTGCGA TTTGCCCTGT GCTCCCACAG GGCCTTGGGG
    GGCGATGTAC ACTTGTCCGT CTCTGAGGTC TTGAGTTCCC GGCATTTCTG CAACAAAATC
    TGGAATGCCC TGCGTTTTAT CCTCAATGCC TTAGGAGAGG GATTTGTACC GCAGCCTGCA
    GAGGAGCTAT CCCCCTCATC CCCCACGGAC GCCTGGATCC TGAGCCGTCT GGCCCACACT
    GCCCAGGAGT GTGAGCGAGC CTTCCTTGCT CGGGAGCTGC CACTCATCAC CCATGCCCTC
    CACCACTTCT GGCTCCACAG CCTCTGTGAT GTCTACTTGG AAGCTGTGAA GCCAGTGCTT
    TCGCACTCGC CCCATCCCCA AGGCCCGCCT CAGGTCCTGT TCTCCTGCGC TGATGTGGGT
    CTCCGCCTCC TCGCCCCACT GATGCCCTTC CTGGCTGAAG AGCTCTGGCA GAGGCTGCCA
    CCCAGGCCTG GCAGCAACTC TGCCCCTAGC ATCTCTGTTG CTCCCTACCC CACCACCCAT
    AGCTTGGAGC ACTGGCGCCA GCCTGCGCTG GAACAACACT TCTCTCAGGT CCAGGAGGCT
    GTGCAGGTAC TGAGGGCCCT CCGAGCCACG TACCAGCTCA CCAAGGCCCG GCCCCGAGTA
    CTGCTGCAGA GCTCAGAGCC TGGAGAGCTG AGCCTCTTCA AGCCCTTCCT GGAGCCCTTG
    GCCACACTGG GCCACTGTGG GGCCGTGGGC CTGTTACCCC CAGGTGTAGC GGCACCCTCC
    GGCTGGGCTC AGGCCCCACT CAGTGACACC ATTCAGGTGT ACATGGAGCT ACAGGGCCTG
    GTGGACCCCC AGGCCCATCT GGCTCTGCTA GCTGCCCGAA GACGCAAGTT GCAGAAGCAG
    CTGGATGGCC TCATGGCCTG GACCCCATCA GGGGGAGAGG AAGAGACTAA GAGGCAGCAG
    AGGCTTTCTT CCCTCCAGCT GGAGTTGTCA AAGCTGGACA AGGCAGCCTC TCACCTCCGG
    CAGCTGACGG ATGCATGTCC CAGCCCCAAG GAGCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005627178.1
    CDS161..3439
    Translation

    Target ORF information:

    RefSeq Version XM_005627121.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005627121.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCCTCGTT CGCCCTTGGC CTCGCTTCCA TCACGGTGCT GGGGGCTCGG GCCCTCTCAG 
    GCCCTCCGCA GGTCCCACCC TCTCTCTACC CAGTCAGAGC CGTGCCGGTC CTCCCTCTCC
    CGGAGGAACC ATGAAGCCAA ACAGAAGCGC CAGCGGGAGA AGCAGATGGC GCTGGAGGCT
    GGGATCACCC CGAAGGGCAA GGCACCTGCA GGATCCAGTA AGGCCTGGAC TCCTAAGGAG
    GTAGTGTTGT ATGAAGTCCC CACGGAACCG GGTGAAAAGA AAGATGTGTA CCGCCTCCTG
    CCTCCTGCAT ACAGTCCTCG CTACGTTGAG GCGGCCTGGT ACTCCTGGTG GGTGCGAGAG
    GGCTTCTTCA AACCGGAGTA TCAGACCAGG CTGCCCCAGG CCACAGGGGA GACGTTTTCC
    ATGTGTATCC CACCTCCCAA TGTCACTGGC TCCCTGCATA TCGGTCATGC ACTGACAGTA
    GCCATACAGG ATGCCCTGGT GCGCTGGCAC CGGATGCGTG GGGATCAGGT GCTATGGATC
    CCTGGCTCAG ATCATGCAGG AATTGCTACA CAAGCTGTGG TGGAGAAGCA ACTGTGGAAG
    GAGCGAGGAA TGAGGAGACA TGAACTGAGC CGGGAAAAAT TCCTTAGGGA GGTGTGGAAG
    TGGAAGGACG AGAAAGGTGG AGAGATCAGT GAGCAGCTCC AAGCTCTGGG GGCCTCCCTG
    GACTGGGACC GAGAGTGCTT CACTATGGAT GCTGGCTTCT CAGTGGCCGT GACCGAAGCT
    TTTGTGCGAC TCTACGAGGC AGGATTGTTG TACCGGAGCC AGCAGCTTGT CAACTGGTCC
    TGCACTTTAA GATCAGCCAT CTCGGATATT GAGGTGGAGA GCCGGCCCCT GCCTGGCCAC
    ACGGAGCTTC AACTGCCTGG CTGCCCCACT CCTGTGTCTT TTGGCCTCCT TGTGTCTGTG
    GCCTTCCCTG TGGATGGAGA GCCTGATGCA GAGGTCGTGG TAGGAACTAC AAGGCCAGAG
    ACGTTGCCTG GAGACGTTGC TGTGGCTGTT CATCCCGACG ACTCCCGATA CACACACCTG
    CATGGGCGAC AGCTTCGTCA CCCCTTGACT GGGCAGCTTC TCCCCCTCAT CACAGACTCC
    ACTGTTCAGC CACATGTGGG CACAGGGGCA GTGAAGGTGA CTCCAGCGCA TAGCCCTGCT
    GATGCTGAGC TGGGGGCCCG ACACGGCTTG AGCCCTCTGA GTGTCATCGC GGAGGATGGC
    ACCATGACCT CTCTCTGTGG GGACTGGCTA CAGGGTCTTC ACCGATTTGT GGCCCGGGAA
    AAGATTATAT CTGCGCTGCG GGAGCGGGGC CTGTTCCGGG GCCTCCAGAA CCACCCCATG
    GTGCTTCCTA TTTGCAGCCG TTCTGGGGAC GTGATAGAAT ATCTACTGAA GAGCCAGTGG
    TTTGTCCGCT GCCGGGAGAT GGGGGACCGA GCTGCCCAGA TGCAAGTGAG GTGCAGGAAG
    GGAAGCTCTA ACGGCCTTTC TGTTCAGGCC GTGGAGTCAG GGGCTCTGGA GCTCAGTCCA
    TCCTTCCACC AGAAGAACTG GCAGCACTGG TTTTCCCACA TCGGGGACTG GTGTGTCTCC
    CGGCAGCTGT GGTGGGGCCA TCGGATTCCC GCCTACCTGG TTGTAGAGGA GCACATGAAG
    GGTGATGGAG AAGACTGTTG GGTGGTTGGG CGGACAGAGA CCGAGGCCAG AAAAATAGCT
    GCAGAACTGA CTGGGAGATC AGAGGCAGAG CTGACCCTGC AGAGGGATCC TGATGTCCTG
    GACACATGGT TCTCTTCGGC TCTTTTCCCC TTTGCTGCCT TGGGCTGGCC CCAAGAGACC
    CCAGACCTTG CTCGTTTCTA CCCCCTGTCA CTTTTGGAAA CAGGCAGTGA TCTCCTGTTG
    TTCTGGGTGG GCCGCATGGT CATGTTGGGG ACACAGCTCA CAGGGCAGCT CCCCTTCACC
    AAGGTGCTTC TTCATTCCAT GGTTCGGGAC GGGCAGGGCC GGAAGATGAG CAAGTCCCTG
    GGGAATGTAT TGGACCCACG AGACATCATC AGTGGGGTGG AACTGCAGGT GCTGCAGGAA
    AAGCTGAAGG ACGGGAACTT GGACTCCACA GAGCTGGCGA TTGCGGCTGC GGCACAGCTG
    TGTAGGCATC TGCCACCCTT CTTATCCCTC TGGTTGCAGA GGAAGGACTT CCCTCATGGG
    ATCCCTGAGT GTGGGACAGA TGCCCTGCGA TTTGCCCTGT GCTCCCACAG GGCCTTGGGG
    GGCGATGTAC ACTTGTCCGT CTCTGAGGTC TTGAGTTCCC GGCATTTCTG CAACAAAATC
    TGGAATGCCC TGCGTTTTAT CCTCAATGCC TTAGGAGAGG GATTTGTACC GCAGCCTGCA
    GAGGAGCTAT CCCCCTCATC CCCCACGGAC GCCTGGATCC TGAGCCGTCT GGCCCACACT
    GCCCAGGAGT GTGAGCGAGC CTTCCTTGCT CGGGAGCTGC CACTCATCAC CCATGCCCTC
    CACCACTTCT GGCTCCACAG CCTCTGTGAT GTCTACTTGG AAGCTGTGAA GCCAGTGCTT
    TCGCACTCGC CCCATCCCCA AGGCCCGCCT CAGGTCCTGT TCTCCTGCGC TGATGTGGGT
    CTCCGCCTCC TCGCCCCACT GATGCCCTTC CTGGCTGAAG AGCTCTGGCA GAGGCTGCCA
    CCCAGGCCTG GCAGCAACTC TGCCCCTAGC ATCTCTGTTG CTCCCTACCC CACCACCCAT
    AGCTTGGAGC ACTGGCGCCA GCCTGCGCTG GAACAACACT TCTCTCAGGT CCAGGAGGCT
    GTGCAGGTAC TGAGGGCCCT CCGAGCCACG TACCAGCTCA CCAAGGCCCG GCCCCGAGTA
    CTGCTGCAGA GCTCAGAGCC TGGAGAGCTG AGCCTCTTCA AGCCCTTCCT GGAGCCCTTG
    GCCACACTGG GCCACTGTGG GGCCGTGGGC CTGTTACCCC CAGGTGTAGC GGCACCCTCC
    GGCTGGGCTC AGGCCCCACT CAGTGACACC ATTCAGGTGT ACATGGAGCT ACAGGGCCTG
    GTGGACCCCC AGGCCCATCT GGCTCTGCTA GCTGCCCGAA GACGCAAGTT GCAGAAGCAG
    CTGGATGGCC TCATGGCCTG GACCCCATCA GGGGGAGAGG AAGAGACTAA GAGGCAGCAG
    AGGCTTTCTT CCCTCCAGCT GGAGTTGTCA AAGCTGGACA AGGCAGCCTC TCACCTCCGG
    CAGCTGACGG ATGCATGTCC CAGCCCCAAG GAGCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe14991
    Clone ID Related Accession (Same CDS sequence) XM_005627121.2 , XM_005627121.3
    Accession Version XM_005627121.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3279bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product valine--tRNA ligase, mitochondrial isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006594.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005627121.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCCTCGTT CGCCCTTGGC CTCGCTTCCA TCACGGTGCT GGGGGCTCGG GCCCTCTCAG 
    GCCCTCCGCA GGTCCCACCC TCTCTCTACC CAGTCAGAGC CGTGCCGGTC CTCCCTCTCC
    CGGAGGAACC ATGAAGCCAA ACAGAAGCGC CAGCGGGAGA AGCAGATGGC GCTGGAGGCT
    GGGATCACCC CGAAGGGCAA GGCACCTGCA GGATCCAGTA AGGCCTGGAC TCCTAAGGAG
    GTAGTGTTGT ATGAAGTCCC CACGGAACCG GGTGAAAAGA AAGATGTGTA CCGCCTCCTG
    CCTCCTGCAT ACAGTCCTCG CTACGTTGAG GCGGCCTGGT ACTCCTGGTG GGTGCGAGAG
    GGCTTCTTCA AACCGGAGTA TCAGACCAGG CTGCCCCAGG CCACAGGGGA GACGTTTTCC
    ATGTGTATCC CACCTCCCAA TGTCACTGGC TCCCTGCATA TCGGTCATGC ACTGACAGTA
    GCCATACAGG ATGCCCTGGT GCGCTGGCAC CGGATGCGTG GGGATCAGGT GCTATGGATC
    CCTGGCTCAG ATCATGCAGG AATTGCTACA CAAGCTGTGG TGGAGAAGCA ACTGTGGAAG
    GAGCGAGGAA TGAGGAGACA TGAACTGAGC CGGGAAAAAT TCCTTAGGGA GGTGTGGAAG
    TGGAAGGACG AGAAAGGTGG AGAGATCAGT GAGCAGCTCC AAGCTCTGGG GGCCTCCCTG
    GACTGGGACC GAGAGTGCTT CACTATGGAT GCTGGCTTCT CAGTGGCCGT GACCGAAGCT
    TTTGTGCGAC TCTACGAGGC AGGATTGTTG TACCGGAGCC AGCAGCTTGT CAACTGGTCC
    TGCACTTTAA GATCAGCCAT CTCGGATATT GAGGTGGAGA GCCGGCCCCT GCCTGGCCAC
    ACGGAGCTTC AACTGCCTGG CTGCCCCACT CCTGTGTCTT TTGGCCTCCT TGTGTCTGTG
    GCCTTCCCTG TGGATGGAGA GCCTGATGCA GAGGTCGTGG TAGGAACTAC AAGGCCAGAG
    ACGTTGCCTG GAGACGTTGC TGTGGCTGTT CATCCCGACG ACTCCCGATA CACACACCTG
    CATGGGCGAC AGCTTCGTCA CCCCTTGACT GGGCAGCTTC TCCCCCTCAT CACAGACTCC
    ACTGTTCAGC CACATGTGGG CACAGGGGCA GTGAAGGTGA CTCCAGCGCA TAGCCCTGCT
    GATGCTGAGC TGGGGGCCCG ACACGGCTTG AGCCCTCTGA GTGTCATCGC GGAGGATGGC
    ACCATGACCT CTCTCTGTGG GGACTGGCTA CAGGGTCTTC ACCGATTTGT GGCCCGGGAA
    AAGATTATAT CTGCGCTGCG GGAGCGGGGC CTGTTCCGGG GCCTCCAGAA CCACCCCATG
    GTGCTTCCTA TTTGCAGCCG TTCTGGGGAC GTGATAGAAT ATCTACTGAA GAGCCAGTGG
    TTTGTCCGCT GCCGGGAGAT GGGGGACCGA GCTGCCCAGA TGCAAGTGAG GTGCAGGAAG
    GGAAGCTCTA ACGGCCTTTC TGTTCAGGCC GTGGAGTCAG GGGCTCTGGA GCTCAGTCCA
    TCCTTCCACC AGAAGAACTG GCAGCACTGG TTTTCCCACA TCGGGGACTG GTGTGTCTCC
    CGGCAGCTGT GGTGGGGCCA TCGGATTCCC GCCTACCTGG TTGTAGAGGA GCACATGAAG
    GGTGATGGAG AAGACTGTTG GGTGGTTGGG CGGACAGAGA CCGAGGCCAG AAAAATAGCT
    GCAGAACTGA CTGGGAGATC AGAGGCAGAG CTGACCCTGC AGAGGGATCC TGATGTCCTG
    GACACATGGT TCTCTTCGGC TCTTTTCCCC TTTGCTGCCT TGGGCTGGCC CCAAGAGACC
    CCAGACCTTG CTCGTTTCTA CCCCCTGTCA CTTTTGGAAA CAGGCAGTGA TCTCCTGTTG
    TTCTGGGTGG GCCGCATGGT CATGTTGGGG ACACAGCTCA CAGGGCAGCT CCCCTTCACC
    AAGGTGCTTC TTCATTCCAT GGTTCGGGAC GGGCAGGGCC GGAAGATGAG CAAGTCCCTG
    GGGAATGTAT TGGACCCACG AGACATCATC AGTGGGGTGG AACTGCAGGT GCTGCAGGAA
    AAGCTGAAGG ACGGGAACTT GGACTCCACA GAGCTGGCGA TTGCGGCTGC GGCACAGCTG
    TGTAGGCATC TGCCACCCTT CTTATCCCTC TGGTTGCAGA GGAAGGACTT CCCTCATGGG
    ATCCCTGAGT GTGGGACAGA TGCCCTGCGA TTTGCCCTGT GCTCCCACAG GGCCTTGGGG
    GGCGATGTAC ACTTGTCCGT CTCTGAGGTC TTGAGTTCCC GGCATTTCTG CAACAAAATC
    TGGAATGCCC TGCGTTTTAT CCTCAATGCC TTAGGAGAGG GATTTGTACC GCAGCCTGCA
    GAGGAGCTAT CCCCCTCATC CCCCACGGAC GCCTGGATCC TGAGCCGTCT GGCCCACACT
    GCCCAGGAGT GTGAGCGAGC CTTCCTTGCT CGGGAGCTGC CACTCATCAC CCATGCCCTC
    CACCACTTCT GGCTCCACAG CCTCTGTGAT GTCTACTTGG AAGCTGTGAA GCCAGTGCTT
    TCGCACTCGC CCCATCCCCA AGGCCCGCCT CAGGTCCTGT TCTCCTGCGC TGATGTGGGT
    CTCCGCCTCC TCGCCCCACT GATGCCCTTC CTGGCTGAAG AGCTCTGGCA GAGGCTGCCA
    CCCAGGCCTG GCAGCAACTC TGCCCCTAGC ATCTCTGTTG CTCCCTACCC CACCACCCAT
    AGCTTGGAGC ACTGGCGCCA GCCTGCGCTG GAACAACACT TCTCTCAGGT CCAGGAGGCT
    GTGCAGGTAC TGAGGGCCCT CCGAGCCACG TACCAGCTCA CCAAGGCCCG GCCCCGAGTA
    CTGCTGCAGA GCTCAGAGCC TGGAGAGCTG AGCCTCTTCA AGCCCTTCCT GGAGCCCTTG
    GCCACACTGG GCCACTGTGG GGCCGTGGGC CTGTTACCCC CAGGTGTAGC GGCACCCTCC
    GGCTGGGCTC AGGCCCCACT CAGTGACACC ATTCAGGTGT ACATGGAGCT ACAGGGCCTG
    GTGGACCCCC AGGCCCATCT GGCTCTGCTA GCTGCCCGAA GACGCAAGTT GCAGAAGCAG
    CTGGATGGCC TCATGGCCTG GACCCCATCA GGGGGAGAGG AAGAGACTAA GAGGCAGCAG
    AGGCTTTCTT CCCTCCAGCT GGAGTTGTCA AAGCTGGACA AGGCAGCCTC TCACCTCCGG
    CAGCTGACGG ATGCATGTCC CAGCCCCAAG GAGCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005627178.1
    CDS126..3404
    Translation

    Target ORF information:

    RefSeq Version XM_005627121.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005627121.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCCTCGTT CGCCCTTGGC CTCGCTTCCA TCACGGTGCT GGGGGCTCGG GCCCTCTCAG 
    GCCCTCCGCA GGTCCCACCC TCTCTCTACC CAGTCAGAGC CGTGCCGGTC CTCCCTCTCC
    CGGAGGAACC ATGAAGCCAA ACAGAAGCGC CAGCGGGAGA AGCAGATGGC GCTGGAGGCT
    GGGATCACCC CGAAGGGCAA GGCACCTGCA GGATCCAGTA AGGCCTGGAC TCCTAAGGAG
    GTAGTGTTGT ATGAAGTCCC CACGGAACCG GGTGAAAAGA AAGATGTGTA CCGCCTCCTG
    CCTCCTGCAT ACAGTCCTCG CTACGTTGAG GCGGCCTGGT ACTCCTGGTG GGTGCGAGAG
    GGCTTCTTCA AACCGGAGTA TCAGACCAGG CTGCCCCAGG CCACAGGGGA GACGTTTTCC
    ATGTGTATCC CACCTCCCAA TGTCACTGGC TCCCTGCATA TCGGTCATGC ACTGACAGTA
    GCCATACAGG ATGCCCTGGT GCGCTGGCAC CGGATGCGTG GGGATCAGGT GCTATGGATC
    CCTGGCTCAG ATCATGCAGG AATTGCTACA CAAGCTGTGG TGGAGAAGCA ACTGTGGAAG
    GAGCGAGGAA TGAGGAGACA TGAACTGAGC CGGGAAAAAT TCCTTAGGGA GGTGTGGAAG
    TGGAAGGACG AGAAAGGTGG AGAGATCAGT GAGCAGCTCC AAGCTCTGGG GGCCTCCCTG
    GACTGGGACC GAGAGTGCTT CACTATGGAT GCTGGCTTCT CAGTGGCCGT GACCGAAGCT
    TTTGTGCGAC TCTACGAGGC AGGATTGTTG TACCGGAGCC AGCAGCTTGT CAACTGGTCC
    TGCACTTTAA GATCAGCCAT CTCGGATATT GAGGTGGAGA GCCGGCCCCT GCCTGGCCAC
    ACGGAGCTTC AACTGCCTGG CTGCCCCACT CCTGTGTCTT TTGGCCTCCT TGTGTCTGTG
    GCCTTCCCTG TGGATGGAGA GCCTGATGCA GAGGTCGTGG TAGGAACTAC AAGGCCAGAG
    ACGTTGCCTG GAGACGTTGC TGTGGCTGTT CATCCCGACG ACTCCCGATA CACACACCTG
    CATGGGCGAC AGCTTCGTCA CCCCTTGACT GGGCAGCTTC TCCCCCTCAT CACAGACTCC
    ACTGTTCAGC CACATGTGGG CACAGGGGCA GTGAAGGTGA CTCCAGCGCA TAGCCCTGCT
    GATGCTGAGC TGGGGGCCCG ACACGGCTTG AGCCCTCTGA GTGTCATCGC GGAGGATGGC
    ACCATGACCT CTCTCTGTGG GGACTGGCTA CAGGGTCTTC ACCGATTTGT GGCCCGGGAA
    AAGATTATAT CTGCGCTGCG GGAGCGGGGC CTGTTCCGGG GCCTCCAGAA CCACCCCATG
    GTGCTTCCTA TTTGCAGCCG TTCTGGGGAC GTGATAGAAT ATCTACTGAA GAGCCAGTGG
    TTTGTCCGCT GCCGGGAGAT GGGGGACCGA GCTGCCCAGA TGCAAGTGAG GTGCAGGAAG
    GGAAGCTCTA ACGGCCTTTC TGTTCAGGCC GTGGAGTCAG GGGCTCTGGA GCTCAGTCCA
    TCCTTCCACC AGAAGAACTG GCAGCACTGG TTTTCCCACA TCGGGGACTG GTGTGTCTCC
    CGGCAGCTGT GGTGGGGCCA TCGGATTCCC GCCTACCTGG TTGTAGAGGA GCACATGAAG
    GGTGATGGAG AAGACTGTTG GGTGGTTGGG CGGACAGAGA CCGAGGCCAG AAAAATAGCT
    GCAGAACTGA CTGGGAGATC AGAGGCAGAG CTGACCCTGC AGAGGGATCC TGATGTCCTG
    GACACATGGT TCTCTTCGGC TCTTTTCCCC TTTGCTGCCT TGGGCTGGCC CCAAGAGACC
    CCAGACCTTG CTCGTTTCTA CCCCCTGTCA CTTTTGGAAA CAGGCAGTGA TCTCCTGTTG
    TTCTGGGTGG GCCGCATGGT CATGTTGGGG ACACAGCTCA CAGGGCAGCT CCCCTTCACC
    AAGGTGCTTC TTCATTCCAT GGTTCGGGAC GGGCAGGGCC GGAAGATGAG CAAGTCCCTG
    GGGAATGTAT TGGACCCACG AGACATCATC AGTGGGGTGG AACTGCAGGT GCTGCAGGAA
    AAGCTGAAGG ACGGGAACTT GGACTCCACA GAGCTGGCGA TTGCGGCTGC GGCACAGCTG
    TGTAGGCATC TGCCACCCTT CTTATCCCTC TGGTTGCAGA GGAAGGACTT CCCTCATGGG
    ATCCCTGAGT GTGGGACAGA TGCCCTGCGA TTTGCCCTGT GCTCCCACAG GGCCTTGGGG
    GGCGATGTAC ACTTGTCCGT CTCTGAGGTC TTGAGTTCCC GGCATTTCTG CAACAAAATC
    TGGAATGCCC TGCGTTTTAT CCTCAATGCC TTAGGAGAGG GATTTGTACC GCAGCCTGCA
    GAGGAGCTAT CCCCCTCATC CCCCACGGAC GCCTGGATCC TGAGCCGTCT GGCCCACACT
    GCCCAGGAGT GTGAGCGAGC CTTCCTTGCT CGGGAGCTGC CACTCATCAC CCATGCCCTC
    CACCACTTCT GGCTCCACAG CCTCTGTGAT GTCTACTTGG AAGCTGTGAA GCCAGTGCTT
    TCGCACTCGC CCCATCCCCA AGGCCCGCCT CAGGTCCTGT TCTCCTGCGC TGATGTGGGT
    CTCCGCCTCC TCGCCCCACT GATGCCCTTC CTGGCTGAAG AGCTCTGGCA GAGGCTGCCA
    CCCAGGCCTG GCAGCAACTC TGCCCCTAGC ATCTCTGTTG CTCCCTACCC CACCACCCAT
    AGCTTGGAGC ACTGGCGCCA GCCTGCGCTG GAACAACACT TCTCTCAGGT CCAGGAGGCT
    GTGCAGGTAC TGAGGGCCCT CCGAGCCACG TACCAGCTCA CCAAGGCCCG GCCCCGAGTA
    CTGCTGCAGA GCTCAGAGCC TGGAGAGCTG AGCCTCTTCA AGCCCTTCCT GGAGCCCTTG
    GCCACACTGG GCCACTGTGG GGCCGTGGGC CTGTTACCCC CAGGTGTAGC GGCACCCTCC
    GGCTGGGCTC AGGCCCCACT CAGTGACACC ATTCAGGTGT ACATGGAGCT ACAGGGCCTG
    GTGGACCCCC AGGCCCATCT GGCTCTGCTA GCTGCCCGAA GACGCAAGTT GCAGAAGCAG
    CTGGATGGCC TCATGGCCTG GACCCCATCA GGGGGAGAGG AAGAGACTAA GAGGCAGCAG
    AGGCTTTCTT CCCTCCAGCT GGAGTTGTCA AAGCTGGACA AGGCAGCCTC TCACCTCCGG
    CAGCTGACGG ATGCATGTCC CAGCCCCAAG GAGCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.