Vars2 cDNA ORF clone, Mus musculus(house mouse)

The following Vars2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Vars2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu53980 XM_030250036.1
Latest version!
Mus musculus valyl-tRNA synthetase 2, mitochondrial (Vars2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OMu70354 XM_011246589.3
Latest version!
Mus musculus valyl-tRNA synthetase 2, mitochondrial (Vars2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OMu21126 NM_175137.4
Latest version!
Mus musculus valyl-tRNA synthetase 2, mitochondrial (Vars2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $622.30
$889.00
View Old Accession Versions >>
OMu70354 XM_011246589.2 Mus musculus valyl-tRNA synthetase 2, mitochondrial (Vars2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu70354
    Clone ID Related Accession (Same CDS sequence) XM_011246589.2 , XM_011246589.3
    Accession Version XM_011246589.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2763bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product valine--tRNA ligase, mitochondrial isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011246589.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGTGTATCC CACCTCCCAA TGTCACCGGC TCCCTGCACA TTGGCCATGC ACTCACAGTG 
    GCGATCCAGG ACGCACTTGT GCGCTGGCAC CGGATGCGTG GGGATCGCGT GCTGTGGATC
    CCCGGGTCTG ATCACGCAGG AATTGCTACA CAGGCCATGG TGGAGAAGCA GCTATGGAAG
    GAGCAGAGGG TGCGGAGACA TGAGCTGAGC CGGGAAGACT TCCTCAGGGC CGTGTGGCAG
    TGGAAACATG AGAAAGGAGG GGAGATCTAT GAACAGCTGT GTGCTCTGGG TGCCTCTCTG
    GACTGGGATC GAGAGTGTTT CACCATGGAT GCCGGCTCCT CAGCAGCTGT GACGGAAGCT
    TTCGTGCGAC TCTACAACTC GGGATTGTTG TACCGGAACC GCCAGCTTGT CAACTGGTCA
    TGTACTTTGC GATCGGCCAT CTCAGACATT GAGGTGGAAA GCCGGCCTCT GCCTGGCCGC
    ACGGTGCTGC AGCTGCCCGG ATGCCCAACC CCTGTGTCTT TTGGGCTCCT TGCTTCTGTT
    GCTTTCCCTG TGGATGGAGA GCCTGACACA GAGATTGTGG TGGGAACCAC CAGGCCAGAG
    ACCCTGCCTG GAGATGTGGC TGTGGCCGTT CATCCAGATG ACCCGAGATA CACGCATTTA
    CATGGGCGAC AGCTCCGTCA TCCTTTGACA GGACAGCTTC TCCCCCTCAT CACAGACACC
    ACTGTTCAGC CACATGTGGG CACAGGGGCA GTGAAGGTGA CTCCTGCTCA CAGTCCAATA
    GATGCTGAGA TAGGGACCCG GCATGGCCTG ACCCCACTGA GTGTCATTGC AGAGGATGGG
    ACCATGACAT CCCTCTGTGG AGACTGGCTG CAGGGCCTTC ATCGATTTGT GGCCCGGGAA
    AAGATCATGT GCACACTGAG GGAGCAGGGC CTATTCCGGG GCCTACAGGA GCACCCCATG
    GTACTGCCCA TCTGCAGCCG TTCCGGGGAC GTGGTGGAGT ACCTGCTGAA GAGCCAGTGG
    TTTGTCCGCT GCCAGGAAAT GGGGGACCTA GCTGCCAAGG CTGTGGAGTC AGGGGCTCTG
    GAGCTCTGGC CCTCCTTCCA CCAGAAGAGC TGGCAACACT GGTTTGCCCA CATTGGGGAC
    TGGTGTGTCT CCCGGCAGCT GTGGTGGGGC CATCAGATTC CAGCCTACCG GGTCATCGGG
    GAGAATGCAG AGGATGACAG GAAGGAATGT TGGGTGGTCG GACGGTCAGA GGCTGAGGCC
    AGAGCGGTAG CAGCAAAACG GACAGGGAGA CCAGAGGCAG AGCTGACCCT GGAGAGGGAC
    CCTGACGTCC TGGACACTTG GTTCTCTTCG GCTCTTTTCC CCTTTTCTGC CCTGGGTTGG
    CCGCGAGAGA CGCCAGACCT TGCTCACTTC TACCCCCTCA CCCTCTTGGA AACGGGTAGT
    GACCTGCTGA TGTTCTGGGT GGGCCGCATG GTCATGCTGG GGACCCAGCT CACTGGGCAG
    CTCCCCTTCA GCAAGGTGCT TCTTCACTCC ATGGTTCGGG ACAGGCAGGG CCGGAAGATG
    AGCAAGTCCC TGGGGAACGT GCTAGACCCA CGGGACATCA TTAGCGGGCA GGAGCTGCAG
    GTGCTGCAGG CCAAGCTGAG AGACGGTAAC TTGGACCCTG GAGAGCTCGC CGTTGCAGCC
    GCAGCACAGA AAAAGGACTT TCCTTACGGG ATCCCTGAGT GTGGGACGGA TGCCCTGAGA
    TTTGCACTGT GCTCCCATGG AATCCTGGGG GGCGACCTGC ACCTGTCTGT CTCTGAGGTC
    CTGAACTACC GCCATTTCTG CAACAAGCTC TGGAATGCCC TGCGCTTTGT CCTCCGTGCC
    CTGGGGGATA ACTTCGTGCC CCAACCAGCA GAGGAGGTAA CCCCCTCCTC TCCCATGGAT
    GCCTGGATTC TAAGCCGCCT GGCCTTTGCA GCCAGCGAAT GTGAGAGAGG CTTCCTGAGT
    CGGGAGCTTT CGCTTGTCAC CCACACCCTG TATCATTTCT GGCTCCACAA TCTCTGTGAT
    GTCTACCTGG AAGCAGTGAA GCCAGTGTTG TCGAGCGTGC CCCGTCCCCC AGGACCCCCT
    CAGGTCCTGT TTTCCTGTGC AGACGTGGGT CTACGCCTCC TTGCCCCGCT GATGCCCTTC
    CTGGCCGAGG AGCTGTGGCA GAGGCTGCCC CCCAGGCCAG GCGGCCCCCT TGCCCCCAGC
    ATCTGTGTGG CCCCCTACCC CAGCACCCGC AGCCTGGAGT TCTGGCGCCA GCCAGAGCTG
    GAGCGCTGCT TCTCCAGAGT CCAAGAGGTT GTACAGGCAT TGCGGGCGCT CCGAGCCACC
    TACCAGCTCA CAAAAGCCCG ACCCCAAGTG CTGCTGCAGT GCTCGGACCC AGGAGAACAA
    GGCCTAGTCC AGCCCTTTCT GGAGCCTCTG GGCATCCTGA GCCACTGCGG GGCTGTAGGA
    TTCCTGTCCC CAGGGGCAGC AGCTCCCTCA GGGTGGGCCC TGACCCCACT GGGTGACACC
    ATGAAGATCT ACATGGAGCT GCAGGGCCTG GTGGACCCCC AGAGCCAGCT GCCCCGGCTC
    ACTGCCCGAA GGCAGAAGTT ACAGAAGCAG CTTGATGACC TGTTGAACCG GACCATGTCA
    GAGGGTCTTG CAGAGAGGCA GCAGAGGATT TCCTCCCTCC ATCTGGAATT GTCAAAGCTG
    GACCAGGCAG CCTCCTACCT ACAGCAACTG ATGGATGAGG CTCCCAGTGC CAGGGAGCTC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011244891.1
    CDS788..3550
    Translation

    Target ORF information:

    RefSeq Version XM_011246589.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus valyl-tRNA synthetase 2, mitochondrial (Vars2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011246589.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGTGTATCC CACCTCCCAA TGTCACCGGC TCCCTGCACA TTGGCCATGC ACTCACAGTG 
    GCGATCCAGG ACGCACTTGT GCGCTGGCAC CGGATGCGTG GGGATCGCGT GCTGTGGATC
    CCCGGGTCTG ATCACGCAGG AATTGCTACA CAGGCCATGG TGGAGAAGCA GCTATGGAAG
    GAGCAGAGGG TGCGGAGACA TGAGCTGAGC CGGGAAGACT TCCTCAGGGC CGTGTGGCAG
    TGGAAACATG AGAAAGGAGG GGAGATCTAT GAACAGCTGT GTGCTCTGGG TGCCTCTCTG
    GACTGGGATC GAGAGTGTTT CACCATGGAT GCCGGCTCCT CAGCAGCTGT GACGGAAGCT
    TTCGTGCGAC TCTACAACTC GGGATTGTTG TACCGGAACC GCCAGCTTGT CAACTGGTCA
    TGTACTTTGC GATCGGCCAT CTCAGACATT GAGGTGGAAA GCCGGCCTCT GCCTGGCCGC
    ACGGTGCTGC AGCTGCCCGG ATGCCCAACC CCTGTGTCTT TTGGGCTCCT TGCTTCTGTT
    GCTTTCCCTG TGGATGGAGA GCCTGACACA GAGATTGTGG TGGGAACCAC CAGGCCAGAG
    ACCCTGCCTG GAGATGTGGC TGTGGCCGTT CATCCAGATG ACCCGAGATA CACGCATTTA
    CATGGGCGAC AGCTCCGTCA TCCTTTGACA GGACAGCTTC TCCCCCTCAT CACAGACACC
    ACTGTTCAGC CACATGTGGG CACAGGGGCA GTGAAGGTGA CTCCTGCTCA CAGTCCAATA
    GATGCTGAGA TAGGGACCCG GCATGGCCTG ACCCCACTGA GTGTCATTGC AGAGGATGGG
    ACCATGACAT CCCTCTGTGG AGACTGGCTG CAGGGCCTTC ATCGATTTGT GGCCCGGGAA
    AAGATCATGT GCACACTGAG GGAGCAGGGC CTATTCCGGG GCCTACAGGA GCACCCCATG
    GTACTGCCCA TCTGCAGCCG TTCCGGGGAC GTGGTGGAGT ACCTGCTGAA GAGCCAGTGG
    TTTGTCCGCT GCCAGGAAAT GGGGGACCTA GCTGCCAAGG CTGTGGAGTC AGGGGCTCTG
    GAGCTCTGGC CCTCCTTCCA CCAGAAGAGC TGGCAACACT GGTTTGCCCA CATTGGGGAC
    TGGTGTGTCT CCCGGCAGCT GTGGTGGGGC CATCAGATTC CAGCCTACCG GGTCATCGGG
    GAGAATGCAG AGGATGACAG GAAGGAATGT TGGGTGGTCG GACGGTCAGA GGCTGAGGCC
    AGAGCGGTAG CAGCAAAACG GACAGGGAGA CCAGAGGCAG AGCTGACCCT GGAGAGGGAC
    CCTGACGTCC TGGACACTTG GTTCTCTTCG GCTCTTTTCC CCTTTTCTGC CCTGGGTTGG
    CCGCGAGAGA CGCCAGACCT TGCTCACTTC TACCCCCTCA CCCTCTTGGA AACGGGTAGT
    GACCTGCTGA TGTTCTGGGT GGGCCGCATG GTCATGCTGG GGACCCAGCT CACTGGGCAG
    CTCCCCTTCA GCAAGGTGCT TCTTCACTCC ATGGTTCGGG ACAGGCAGGG CCGGAAGATG
    AGCAAGTCCC TGGGGAACGT GCTAGACCCA CGGGACATCA TTAGCGGGCA GGAGCTGCAG
    GTGCTGCAGG CCAAGCTGAG AGACGGTAAC TTGGACCCTG GAGAGCTCGC CGTTGCAGCC
    GCAGCACAGA AAAAGGACTT TCCTTACGGG ATCCCTGAGT GTGGGACGGA TGCCCTGAGA
    TTTGCACTGT GCTCCCATGG AATCCTGGGG GGCGACCTGC ACCTGTCTGT CTCTGAGGTC
    CTGAACTACC GCCATTTCTG CAACAAGCTC TGGAATGCCC TGCGCTTTGT CCTCCGTGCC
    CTGGGGGATA ACTTCGTGCC CCAACCAGCA GAGGAGGTAA CCCCCTCCTC TCCCATGGAT
    GCCTGGATTC TAAGCCGCCT GGCCTTTGCA GCCAGCGAAT GTGAGAGAGG CTTCCTGAGT
    CGGGAGCTTT CGCTTGTCAC CCACACCCTG TATCATTTCT GGCTCCACAA TCTCTGTGAT
    GTCTACCTGG AAGCAGTGAA GCCAGTGTTG TCGAGCGTGC CCCGTCCCCC AGGACCCCCT
    CAGGTCCTGT TTTCCTGTGC AGACGTGGGT CTACGCCTCC TTGCCCCGCT GATGCCCTTC
    CTGGCCGAGG AGCTGTGGCA GAGGCTGCCC CCCAGGCCAG GCGGCCCCCT TGCCCCCAGC
    ATCTGTGTGG CCCCCTACCC CAGCACCCGC AGCCTGGAGT TCTGGCGCCA GCCAGAGCTG
    GAGCGCTGCT TCTCCAGAGT CCAAGAGGTT GTACAGGCAT TGCGGGCGCT CCGAGCCACC
    TACCAGCTCA CAAAAGCCCG ACCCCAAGTG CTGCTGCAGT GCTCGGACCC AGGAGAACAA
    GGCCTAGTCC AGCCCTTTCT GGAGCCTCTG GGCATCCTGA GCCACTGCGG GGCTGTAGGA
    TTCCTGTCCC CAGGGGCAGC AGCTCCCTCA GGGTGGGCCC TGACCCCACT GGGTGACACC
    ATGAAGATCT ACATGGAGCT GCAGGGCCTG GTGGACCCCC AGAGCCAGCT GCCCCGGCTC
    ACTGCCCGAA GGCAGAAGTT ACAGAAGCAG CTTGATGACC TGTTGAACCG GACCATGTCA
    GAGGGTCTTG CAGAGAGGCA GCAGAGGATT TCCTCCCTCC ATCTGGAATT GTCAAAGCTG
    GACCAGGCAG CCTCCTACCT ACAGCAACTG ATGGATGAGG CTCCCAGTGC CAGGGAGCTC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu53980
    Clone ID Related Accession (Same CDS sequence) XM_030250036.1
    Accession Version XM_030250036.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1668bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product valine--tRNA ligase, mitochondrial isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGCCTCATT TGCCTCTGGC CTCTTTCCGG CCACCGCTCT GGGGGCTGAG GCCCTCATGG 
    GGTCTTTCCA GGCCCCAGGC ACTTTGTACA CAGCCGGAGC CCCACGGCTC CCCTGTCTCT
    CGGAGGAACC GTGAAGCCAA ACAGAAGCGC CTGCGCGAGA AACAGGCGGC CCTGGAGGCT
    GGGCTAGCTG AGAAGAGCAA GATACCTGCA GTGCCCACTA AGGCCTGGAG TCACAAGGAG
    GTAGTATTGT ATGAAATCCC CACCGGGCCA GGTGAAAAGA AAGATGTTTC TGGGCCCCTG
    CCTCCTGCTT ACAGTCCCCA GTATGTTGAA GCGGCCTGGT ACCAGTGGTG GGTGCGAGAG
    GGCTTCTTCA AACCAGAGTA TCAGGCAAGG CTGCCCCAAG CTACAGGGGA GACCTTTTCC
    ATGTGTATCC CACCTCCCAA TGTCACCGGC TCCCTGCACA TTGGCCATGC ACTCACAGTG
    GCGATCCAGG ACGCACTTGT GCGCTGGCAC CGGATGCGTG GGGATCGCGT GCTGTGGATC
    CCCGGGTCTG ATCACGCAGG AATTGCTACA CAGGCCATGG TGGAGAAGCA GCTATGGAAG
    GAGCAGAGGG TGCGGAGACA TGAGCTGAGC CGGGAAGACT TCCTCAGGGC CGTGTGGCAG
    TGGAAACATG AGAAAGGAGG GGAGATCTAT GAACAGCTGT GTGCTCTGGG TGCCTCTCTG
    GACTGGGATC GAGAGTGTTT CACCATGGAT GCCGGCTCCT CAGCAGCTGT GACGGAAGCT
    TTCGTGCGAC TCTACAACTC GGGATTGTTG TACCGGAACC GCCAGCTTGT CAACTGGTCA
    TGTACTTTGC GATCGGCCAT CTCAGACATT GAGGTGGAAA GCCGGCCTCT GCCTGGCCGC
    ACGGTGCTGC AGCTGCCCGG ATGCCCAACC CCTGTGTCTT TTGGGCTCCT TGCTTCTGTT
    GCTTTCCCTG TGGATGGAGA GCCTGACACA GAGATTGTGG TGGGAACCAC CAGGCCAGAG
    ACCCTGCCTG GAGATGTGGC TGTGGCCGTT CATCCAGATG ACCCGAGATA CACGCATTTA
    CATGGGCGAC AGCTCCGTCA TCCTTTGACA GGACAGCTTC TCCCCCTCAT CACAGACACC
    ACTGTTCAGC CACATGTGGG CACAGGGGCA GTGAAGGTGA CTCCTGCTCA CAGTCCAATA
    GATGCTGAGA TAGGGACCCG GCATGGCCTG ACCCCACTGA GTGTCATTGC AGAGGATGGG
    ACCATGACAT CCCTCTGTGG AGACTGGCTG CAGGGCCTTC ATCGATTTGT GGCCCGGGAA
    AAGATCATGT GCACACTGAG GGAGCAGGGC CTATTCCGGG GCCTACAGGA GCACCCCATG
    GTACTGCCCA TCTGCAGCCG TTCCGGGGAC GTGGTGGAGT ACCTGCTGAA GAGCCAGTGG
    TTTGTCCGCT GCCAGGAAAT GGGGGACCTA GCTGCCAAGG CTGTGGAGTC AGGGGCTCTG
    GAGCTCTGGC CCTCCTTCCA CCAGAAGAGC TGGCAACACT GGTTTGCCCA CATTGGCTGT
    GGTGGGGCCA TCAGATTCCA GCCTACCGGG TCATCGGGGA GAATGCAGAG GATGACAGGA
    AGGAATGTTG GGTGGTCGGA CGGTCAGAGG CTGAGGCCAG AGCGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030105896.1
    CDS367..2034
    Translation

    Target ORF information:

    RefSeq Version XM_030250036.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus valyl-tRNA synthetase 2, mitochondrial (Vars2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030250036.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGCCTCATT TGCCTCTGGC CTCTTTCCGG CCACCGCTCT GGGGGCTGAG GCCCTCATGG 
    GGTCTTTCCA GGCCCCAGGC ACTTTGTACA CAGCCGGAGC CCCACGGCTC CCCTGTCTCT
    CGGAGGAACC GTGAAGCCAA ACAGAAGCGC CTGCGCGAGA AACAGGCGGC CCTGGAGGCT
    GGGCTAGCTG AGAAGAGCAA GATACCTGCA GTGCCCACTA AGGCCTGGAG TCACAAGGAG
    GTAGTATTGT ATGAAATCCC CACCGGGCCA GGTGAAAAGA AAGATGTTTC TGGGCCCCTG
    CCTCCTGCTT ACAGTCCCCA GTATGTTGAA GCGGCCTGGT ACCAGTGGTG GGTGCGAGAG
    GGCTTCTTCA AACCAGAGTA TCAGGCAAGG CTGCCCCAAG CTACAGGGGA GACCTTTTCC
    ATGTGTATCC CACCTCCCAA TGTCACCGGC TCCCTGCACA TTGGCCATGC ACTCACAGTG
    GCGATCCAGG ACGCACTTGT GCGCTGGCAC CGGATGCGTG GGGATCGCGT GCTGTGGATC
    CCCGGGTCTG ATCACGCAGG AATTGCTACA CAGGCCATGG TGGAGAAGCA GCTATGGAAG
    GAGCAGAGGG TGCGGAGACA TGAGCTGAGC CGGGAAGACT TCCTCAGGGC CGTGTGGCAG
    TGGAAACATG AGAAAGGAGG GGAGATCTAT GAACAGCTGT GTGCTCTGGG TGCCTCTCTG
    GACTGGGATC GAGAGTGTTT CACCATGGAT GCCGGCTCCT CAGCAGCTGT GACGGAAGCT
    TTCGTGCGAC TCTACAACTC GGGATTGTTG TACCGGAACC GCCAGCTTGT CAACTGGTCA
    TGTACTTTGC GATCGGCCAT CTCAGACATT GAGGTGGAAA GCCGGCCTCT GCCTGGCCGC
    ACGGTGCTGC AGCTGCCCGG ATGCCCAACC CCTGTGTCTT TTGGGCTCCT TGCTTCTGTT
    GCTTTCCCTG TGGATGGAGA GCCTGACACA GAGATTGTGG TGGGAACCAC CAGGCCAGAG
    ACCCTGCCTG GAGATGTGGC TGTGGCCGTT CATCCAGATG ACCCGAGATA CACGCATTTA
    CATGGGCGAC AGCTCCGTCA TCCTTTGACA GGACAGCTTC TCCCCCTCAT CACAGACACC
    ACTGTTCAGC CACATGTGGG CACAGGGGCA GTGAAGGTGA CTCCTGCTCA CAGTCCAATA
    GATGCTGAGA TAGGGACCCG GCATGGCCTG ACCCCACTGA GTGTCATTGC AGAGGATGGG
    ACCATGACAT CCCTCTGTGG AGACTGGCTG CAGGGCCTTC ATCGATTTGT GGCCCGGGAA
    AAGATCATGT GCACACTGAG GGAGCAGGGC CTATTCCGGG GCCTACAGGA GCACCCCATG
    GTACTGCCCA TCTGCAGCCG TTCCGGGGAC GTGGTGGAGT ACCTGCTGAA GAGCCAGTGG
    TTTGTCCGCT GCCAGGAAAT GGGGGACCTA GCTGCCAAGG CTGTGGAGTC AGGGGCTCTG
    GAGCTCTGGC CCTCCTTCCA CCAGAAGAGC TGGCAACACT GGTTTGCCCA CATTGGCTGT
    GGTGGGGCCA TCAGATTCCA GCCTACCGGG TCATCGGGGA GAATGCAGAG GATGACAGGA
    AGGAATGTTG GGTGGTCGGA CGGTCAGAGG CTGAGGCCAG AGCGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu70354
    Clone ID Related Accession (Same CDS sequence) XM_011246589.2 , XM_011246589.3
    Accession Version XM_011246589.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2763bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product valine--tRNA ligase, mitochondrial isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011246589.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGTGTATCC CACCTCCCAA TGTCACCGGC TCCCTGCACA TTGGCCATGC ACTCACAGTG 
    GCGATCCAGG ACGCACTTGT GCGCTGGCAC CGGATGCGTG GGGATCGCGT GCTGTGGATC
    CCCGGGTCTG ATCACGCAGG AATTGCTACA CAGGCCATGG TGGAGAAGCA GCTATGGAAG
    GAGCAGAGGG TGCGGAGACA TGAGCTGAGC CGGGAAGACT TCCTCAGGGC CGTGTGGCAG
    TGGAAACATG AGAAAGGAGG GGAGATCTAT GAACAGCTGT GTGCTCTGGG TGCCTCTCTG
    GACTGGGATC GAGAGTGTTT CACCATGGAT GCCGGCTCCT CAGCAGCTGT GACGGAAGCT
    TTCGTGCGAC TCTACAACTC GGGATTGTTG TACCGGAACC GCCAGCTTGT CAACTGGTCA
    TGTACTTTGC GATCGGCCAT CTCAGACATT GAGGTGGAAA GCCGGCCTCT GCCTGGCCGC
    ACGGTGCTGC AGCTGCCCGG ATGCCCAACC CCTGTGTCTT TTGGGCTCCT TGCTTCTGTT
    GCTTTCCCTG TGGATGGAGA GCCTGACACA GAGATTGTGG TGGGAACCAC CAGGCCAGAG
    ACCCTGCCTG GAGATGTGGC TGTGGCCGTT CATCCAGATG ACCCGAGATA CACGCATTTA
    CATGGGCGAC AGCTCCGTCA TCCTTTGACA GGACAGCTTC TCCCCCTCAT CACAGACACC
    ACTGTTCAGC CACATGTGGG CACAGGGGCA GTGAAGGTGA CTCCTGCTCA CAGTCCAATA
    GATGCTGAGA TAGGGACCCG GCATGGCCTG ACCCCACTGA GTGTCATTGC AGAGGATGGG
    ACCATGACAT CCCTCTGTGG AGACTGGCTG CAGGGCCTTC ATCGATTTGT GGCCCGGGAA
    AAGATCATGT GCACACTGAG GGAGCAGGGC CTATTCCGGG GCCTACAGGA GCACCCCATG
    GTACTGCCCA TCTGCAGCCG TTCCGGGGAC GTGGTGGAGT ACCTGCTGAA GAGCCAGTGG
    TTTGTCCGCT GCCAGGAAAT GGGGGACCTA GCTGCCAAGG CTGTGGAGTC AGGGGCTCTG
    GAGCTCTGGC CCTCCTTCCA CCAGAAGAGC TGGCAACACT GGTTTGCCCA CATTGGGGAC
    TGGTGTGTCT CCCGGCAGCT GTGGTGGGGC CATCAGATTC CAGCCTACCG GGTCATCGGG
    GAGAATGCAG AGGATGACAG GAAGGAATGT TGGGTGGTCG GACGGTCAGA GGCTGAGGCC
    AGAGCGGTAG CAGCAAAACG GACAGGGAGA CCAGAGGCAG AGCTGACCCT GGAGAGGGAC
    CCTGACGTCC TGGACACTTG GTTCTCTTCG GCTCTTTTCC CCTTTTCTGC CCTGGGTTGG
    CCGCGAGAGA CGCCAGACCT TGCTCACTTC TACCCCCTCA CCCTCTTGGA AACGGGTAGT
    GACCTGCTGA TGTTCTGGGT GGGCCGCATG GTCATGCTGG GGACCCAGCT CACTGGGCAG
    CTCCCCTTCA GCAAGGTGCT TCTTCACTCC ATGGTTCGGG ACAGGCAGGG CCGGAAGATG
    AGCAAGTCCC TGGGGAACGT GCTAGACCCA CGGGACATCA TTAGCGGGCA GGAGCTGCAG
    GTGCTGCAGG CCAAGCTGAG AGACGGTAAC TTGGACCCTG GAGAGCTCGC CGTTGCAGCC
    GCAGCACAGA AAAAGGACTT TCCTTACGGG ATCCCTGAGT GTGGGACGGA TGCCCTGAGA
    TTTGCACTGT GCTCCCATGG AATCCTGGGG GGCGACCTGC ACCTGTCTGT CTCTGAGGTC
    CTGAACTACC GCCATTTCTG CAACAAGCTC TGGAATGCCC TGCGCTTTGT CCTCCGTGCC
    CTGGGGGATA ACTTCGTGCC CCAACCAGCA GAGGAGGTAA CCCCCTCCTC TCCCATGGAT
    GCCTGGATTC TAAGCCGCCT GGCCTTTGCA GCCAGCGAAT GTGAGAGAGG CTTCCTGAGT
    CGGGAGCTTT CGCTTGTCAC CCACACCCTG TATCATTTCT GGCTCCACAA TCTCTGTGAT
    GTCTACCTGG AAGCAGTGAA GCCAGTGTTG TCGAGCGTGC CCCGTCCCCC AGGACCCCCT
    CAGGTCCTGT TTTCCTGTGC AGACGTGGGT CTACGCCTCC TTGCCCCGCT GATGCCCTTC
    CTGGCCGAGG AGCTGTGGCA GAGGCTGCCC CCCAGGCCAG GCGGCCCCCT TGCCCCCAGC
    ATCTGTGTGG CCCCCTACCC CAGCACCCGC AGCCTGGAGT TCTGGCGCCA GCCAGAGCTG
    GAGCGCTGCT TCTCCAGAGT CCAAGAGGTT GTACAGGCAT TGCGGGCGCT CCGAGCCACC
    TACCAGCTCA CAAAAGCCCG ACCCCAAGTG CTGCTGCAGT GCTCGGACCC AGGAGAACAA
    GGCCTAGTCC AGCCCTTTCT GGAGCCTCTG GGCATCCTGA GCCACTGCGG GGCTGTAGGA
    TTCCTGTCCC CAGGGGCAGC AGCTCCCTCA GGGTGGGCCC TGACCCCACT GGGTGACACC
    ATGAAGATCT ACATGGAGCT GCAGGGCCTG GTGGACCCCC AGAGCCAGCT GCCCCGGCTC
    ACTGCCCGAA GGCAGAAGTT ACAGAAGCAG CTTGATGACC TGTTGAACCG GACCATGTCA
    GAGGGTCTTG CAGAGAGGCA GCAGAGGATT TCCTCCCTCC ATCTGGAATT GTCAAAGCTG
    GACCAGGCAG CCTCCTACCT ACAGCAACTG ATGGATGAGG CTCCCAGTGC CAGGGAGCTC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011244891.1
    CDS794..3556
    Translation

    Target ORF information:

    RefSeq Version XM_011246589.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus valyl-tRNA synthetase 2, mitochondrial (Vars2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011246589.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGTGTATCC CACCTCCCAA TGTCACCGGC TCCCTGCACA TTGGCCATGC ACTCACAGTG 
    GCGATCCAGG ACGCACTTGT GCGCTGGCAC CGGATGCGTG GGGATCGCGT GCTGTGGATC
    CCCGGGTCTG ATCACGCAGG AATTGCTACA CAGGCCATGG TGGAGAAGCA GCTATGGAAG
    GAGCAGAGGG TGCGGAGACA TGAGCTGAGC CGGGAAGACT TCCTCAGGGC CGTGTGGCAG
    TGGAAACATG AGAAAGGAGG GGAGATCTAT GAACAGCTGT GTGCTCTGGG TGCCTCTCTG
    GACTGGGATC GAGAGTGTTT CACCATGGAT GCCGGCTCCT CAGCAGCTGT GACGGAAGCT
    TTCGTGCGAC TCTACAACTC GGGATTGTTG TACCGGAACC GCCAGCTTGT CAACTGGTCA
    TGTACTTTGC GATCGGCCAT CTCAGACATT GAGGTGGAAA GCCGGCCTCT GCCTGGCCGC
    ACGGTGCTGC AGCTGCCCGG ATGCCCAACC CCTGTGTCTT TTGGGCTCCT TGCTTCTGTT
    GCTTTCCCTG TGGATGGAGA GCCTGACACA GAGATTGTGG TGGGAACCAC CAGGCCAGAG
    ACCCTGCCTG GAGATGTGGC TGTGGCCGTT CATCCAGATG ACCCGAGATA CACGCATTTA
    CATGGGCGAC AGCTCCGTCA TCCTTTGACA GGACAGCTTC TCCCCCTCAT CACAGACACC
    ACTGTTCAGC CACATGTGGG CACAGGGGCA GTGAAGGTGA CTCCTGCTCA CAGTCCAATA
    GATGCTGAGA TAGGGACCCG GCATGGCCTG ACCCCACTGA GTGTCATTGC AGAGGATGGG
    ACCATGACAT CCCTCTGTGG AGACTGGCTG CAGGGCCTTC ATCGATTTGT GGCCCGGGAA
    AAGATCATGT GCACACTGAG GGAGCAGGGC CTATTCCGGG GCCTACAGGA GCACCCCATG
    GTACTGCCCA TCTGCAGCCG TTCCGGGGAC GTGGTGGAGT ACCTGCTGAA GAGCCAGTGG
    TTTGTCCGCT GCCAGGAAAT GGGGGACCTA GCTGCCAAGG CTGTGGAGTC AGGGGCTCTG
    GAGCTCTGGC CCTCCTTCCA CCAGAAGAGC TGGCAACACT GGTTTGCCCA CATTGGGGAC
    TGGTGTGTCT CCCGGCAGCT GTGGTGGGGC CATCAGATTC CAGCCTACCG GGTCATCGGG
    GAGAATGCAG AGGATGACAG GAAGGAATGT TGGGTGGTCG GACGGTCAGA GGCTGAGGCC
    AGAGCGGTAG CAGCAAAACG GACAGGGAGA CCAGAGGCAG AGCTGACCCT GGAGAGGGAC
    CCTGACGTCC TGGACACTTG GTTCTCTTCG GCTCTTTTCC CCTTTTCTGC CCTGGGTTGG
    CCGCGAGAGA CGCCAGACCT TGCTCACTTC TACCCCCTCA CCCTCTTGGA AACGGGTAGT
    GACCTGCTGA TGTTCTGGGT GGGCCGCATG GTCATGCTGG GGACCCAGCT CACTGGGCAG
    CTCCCCTTCA GCAAGGTGCT TCTTCACTCC ATGGTTCGGG ACAGGCAGGG CCGGAAGATG
    AGCAAGTCCC TGGGGAACGT GCTAGACCCA CGGGACATCA TTAGCGGGCA GGAGCTGCAG
    GTGCTGCAGG CCAAGCTGAG AGACGGTAAC TTGGACCCTG GAGAGCTCGC CGTTGCAGCC
    GCAGCACAGA AAAAGGACTT TCCTTACGGG ATCCCTGAGT GTGGGACGGA TGCCCTGAGA
    TTTGCACTGT GCTCCCATGG AATCCTGGGG GGCGACCTGC ACCTGTCTGT CTCTGAGGTC
    CTGAACTACC GCCATTTCTG CAACAAGCTC TGGAATGCCC TGCGCTTTGT CCTCCGTGCC
    CTGGGGGATA ACTTCGTGCC CCAACCAGCA GAGGAGGTAA CCCCCTCCTC TCCCATGGAT
    GCCTGGATTC TAAGCCGCCT GGCCTTTGCA GCCAGCGAAT GTGAGAGAGG CTTCCTGAGT
    CGGGAGCTTT CGCTTGTCAC CCACACCCTG TATCATTTCT GGCTCCACAA TCTCTGTGAT
    GTCTACCTGG AAGCAGTGAA GCCAGTGTTG TCGAGCGTGC CCCGTCCCCC AGGACCCCCT
    CAGGTCCTGT TTTCCTGTGC AGACGTGGGT CTACGCCTCC TTGCCCCGCT GATGCCCTTC
    CTGGCCGAGG AGCTGTGGCA GAGGCTGCCC CCCAGGCCAG GCGGCCCCCT TGCCCCCAGC
    ATCTGTGTGG CCCCCTACCC CAGCACCCGC AGCCTGGAGT TCTGGCGCCA GCCAGAGCTG
    GAGCGCTGCT TCTCCAGAGT CCAAGAGGTT GTACAGGCAT TGCGGGCGCT CCGAGCCACC
    TACCAGCTCA CAAAAGCCCG ACCCCAAGTG CTGCTGCAGT GCTCGGACCC AGGAGAACAA
    GGCCTAGTCC AGCCCTTTCT GGAGCCTCTG GGCATCCTGA GCCACTGCGG GGCTGTAGGA
    TTCCTGTCCC CAGGGGCAGC AGCTCCCTCA GGGTGGGCCC TGACCCCACT GGGTGACACC
    ATGAAGATCT ACATGGAGCT GCAGGGCCTG GTGGACCCCC AGAGCCAGCT GCCCCGGCTC
    ACTGCCCGAA GGCAGAAGTT ACAGAAGCAG CTTGATGACC TGTTGAACCG GACCATGTCA
    GAGGGTCTTG CAGAGAGGCA GCAGAGGATT TCCTCCCTCC ATCTGGAATT GTCAAAGCTG
    GACCAGGCAG CCTCCTACCT ACAGCAACTG ATGGATGAGG CTCCCAGTGC CAGGGAGCTC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu21126
    Clone ID Related Accession (Same CDS sequence) NM_175137.4
    Accession Version NM_175137.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3183bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-19
    Organism Mus musculus(house mouse)
    Product valine--tRNA ligase, mitochondrial precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK129008.1, CA327239.1, CD355876.1, CX563092.1, AK038117.1, AK004481.1 and BC057036.1. On Nov 15, 2007 this sequence version replaced NM_175137.3. ##Evidence-Data-START## Transcript exon combination :: AK129008.1, AK155386.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGCCTCATT TGCCTCTGGC CTCTTTCCGG CCACCGCTCT GGGGGCTGAG GCCCTCATGG 
    GGTCTTTCCA GGCCCCAGGC ACTTTGTACA CAGCCGGAGC CCCACGGCTC CCCTGTCTCT
    CGGAGGAACC GTGAAGCCAA ACAGAAGCGC CTGCGCGAGA AACAGGCGGC CCTGGAGGCT
    GGGCTAGCTG AGAAGAGCAA GATACCTGCA GTGCCCACTA AGGCCTGGAG TCACAAGGAG
    GTAGTATTGT ATGAAATCCC CACCGGGCCA GGTGAAAAGA AAGATGTTTC TGGGCCCCTG
    CCTCCTGCTT ACAGTCCCCA GTATGTTGAA GCGGCCTGGT ACCAGTGGTG GGTGCGAGAG
    GGCTTCTTCA AACCAGAGTA TCAGGCAAGG CTGCCCCAAG CTACAGGGGA GACCTTTTCC
    ATGTGTATCC CACCTCCCAA TGTCACCGGC TCCCTGCACA TTGGCCATGC ACTCACAGTG
    GCGATCCAGG ACGCACTTGT GCGCTGGCAC CGGATGCGTG GGGATCGCGT GCTGTGGATC
    CCCGGGTCTG ATCACGCAGG AATTGCTACA CAGGCCATGG TGGAGAAGCA GCTATGGAAG
    GAGCAGAGGG TGCGGAGACA TGAGCTGAGC CGGGAAGACT TCCTCAGGGC CGTGTGGCAG
    TGGAAACATG AGAAAGGAGG GGAGATCTAT GAACAGCTGT GTGCTCTGGG TGCCTCTCTG
    GACTGGGATC GAGAGTGTTT CACCATGGAT GCCGGCTCCT CAGCAGCTGT GACGGAAGCT
    TTCGTGCGAC TCTACAACTC GGGATTGTTG TACCGGAACC GCCAGCTTGT CAACTGGTCA
    TGTACTTTGC GATCGGCCAT CTCAGACATT GAGGTGGAAA GCCGGCCTCT GCCTGGCCGC
    ACGGTGCTGC AGCTGCCCGG ATGCCCAACC CCTGTGTCTT TTGGGCTCCT TGCTTCTGTT
    GCTTTCCCTG TGGATGGAGA GCCTGACACA GAGATTGTGG TGGGAACCAC CAGGCCAGAG
    ACCCTGCCTG GAGATGTGGC TGTGGCCGTT CATCCAGATG ACCCGAGATA CACGCATTTA
    CATGGGCGAC AGCTCCGTCA TCCTTTGACA GGACAGCTTC TCCCCCTCAT CACAGACACC
    ACTGTTCAGC CACATGTGGG CACAGGGGCA GTGAAGGTGA CTCCTGCTCA CAGTCCAATA
    GATGCTGAGA TAGGGACCCG GCATGGCCTG ACCCCACTGA GTGTCATTGC AGAGGATGGG
    ACCATGACAT CCCTCTGTGG AGACTGGCTG CAGGGCCTTC ATCGATTTGT GGCCCGGGAA
    AAGATCATGT GCACACTGAG GGAGCAGGGC CTATTCCGGG GCCTACAGGA GCACCCCATG
    GTACTGCCCA TCTGCAGCCG TTCCGGGGAC GTGGTGGAGT ACCTGCTGAA GAGCCAGTGG
    TTTGTCCGCT GCCAGGAAAT GGGGGACCTA GCTGCCAAGG CTGTGGAGTC AGGGGCTCTG
    GAGCTCTGGC CCTCCTTCCA CCAGAAGAGC TGGCAACACT GGTTTGCCCA CATTGGGGAC
    TGGTGTGTCT CCCGGCAGCT GTGGTGGGGC CATCAGATTC CAGCCTACCG GGTCATCGGG
    GAGAATGCAG AGGATGACAG GAAGGAATGT TGGGTGGTCG GACGGTCAGA GGCTGAGGCC
    AGAGCGGTAG CAGCAAAACG GACAGGGAGA CCAGAGGCAG AGCTGACCCT GGAGAGGGAC
    CCTGACGTCC TGGACACTTG GTTCTCTTCG GCTCTTTTCC CCTTTTCTGC CCTGGGTTGG
    CCGCGAGAGA CGCCAGACCT TGCTCACTTC TACCCCCTCA CCCTCTTGGA AACGGGTAGT
    GACCTGCTGA TGTTCTGGGT GGGCCGCATG GTCATGCTGG GGACCCAGCT CACTGGGCAG
    CTCCCCTTCA GCAAGGTGCT TCTTCACTCC ATGGTTCGGG ACAGGCAGGG CCGGAAGATG
    AGCAAGTCCC TGGGGAACGT GCTAGACCCA CGGGACATCA TTAGCGGGCA GGAGCTGCAG
    GTGCTGCAGG CCAAGCTGAG AGACGGTAAC TTGGACCCTG GAGAGCTCGC CGTTGCAGCC
    GCAGCACAGA AAAAGGACTT TCCTTACGGG ATCCCTGAGT GTGGGACGGA TGCCCTGAGA
    TTTGCACTGT GCTCCCATGG AATCCTGGGG GGCGACCTGC ACCTGTCTGT CTCTGAGGTC
    CTGAACTACC GCCATTTCTG CAACAAGCTC TGGAATGCCC TGCGCTTTGT CCTCCGTGCC
    CTGGGGGATA ACTTCGTGCC CCAACCAGCA GAGGAGGTAA CCCCCTCCTC TCCCATGGAT
    GCCTGGATTC TAAGCCGCCT GGCCTTTGCA GCCAGCGAAT GTGAGAGAGG CTTCCTGAGT
    CGGGAGCTTT CGCTTGTCAC CCACACCCTG TATCATTTCT GGCTCCACAA TCTCTGTGAT
    GTCTACCTGG AAGCAGTGAA GCCAGTGTTG TCGAGCGTGC CCCGTCCCCC AGGACCCCCT
    CAGGTCCTGT TTTCCTGTGC AGACGTGGGT CTACGCCTCC TTGCCCCGCT GATGCCCTTC
    CTGGCCGAGG AGCTGTGGCA GAGGCTGCCC CCCAGGCCAG GCGGCCCCCT TGCCCCCAGC
    ATCTGTGTGG CCCCCTACCC CAGCACCCGC AGCCTGGAGT TCTGGCGCCA GCCAGAGCTG
    GAGCGCTGCT TCTCCAGAGT CCAAGAGGTT GTACAGGCAT TGCGGGCGCT CCGAGCCACC
    TACCAGCTCA CAAAAGCCCG ACCCCAAGTG CTGCTGCAGT GCTCGGACCC AGGAGAACAA
    GGCCTAGTCC AGCCCTTTCT GGAGCCTCTG GGCATCCTGA GCCACTGCGG GGCTGTAGGA
    TTCCTGTCCC CAGGGGCAGC AGCTCCCTCA GGGTGGGCCC TGACCCCACT GGGTGACACC
    ATGAAGATCT ACATGGAGCT GCAGGGCCTG GTGGACCCCC AGAGCCAGCT GCCCCGGCTC
    ACTGCCCGAA GGCAGAAGTT ACAGAAGCAG CTTGATGACC TGTTGAACCG GACCATGTCA
    GAGGGTCTTG CAGAGAGGCA GCAGAGGATT TCCTCCCTCC ATCTGGAATT GTCAAAGCTG
    GACCAGGCAG CCTCCTACCT ACAGCAACTG ATGGATGAGG CTCCCAGTGC CAGGGAGCTC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_780346.3
    CDS320..3502
    Translation

    Target ORF information:

    RefSeq Version NM_175137.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus valyl-tRNA synthetase 2, mitochondrial (Vars2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_175137.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGCCTCATT TGCCTCTGGC CTCTTTCCGG CCACCGCTCT GGGGGCTGAG GCCCTCATGG 
    GGTCTTTCCA GGCCCCAGGC ACTTTGTACA CAGCCGGAGC CCCACGGCTC CCCTGTCTCT
    CGGAGGAACC GTGAAGCCAA ACAGAAGCGC CTGCGCGAGA AACAGGCGGC CCTGGAGGCT
    GGGCTAGCTG AGAAGAGCAA GATACCTGCA GTGCCCACTA AGGCCTGGAG TCACAAGGAG
    GTAGTATTGT ATGAAATCCC CACCGGGCCA GGTGAAAAGA AAGATGTTTC TGGGCCCCTG
    CCTCCTGCTT ACAGTCCCCA GTATGTTGAA GCGGCCTGGT ACCAGTGGTG GGTGCGAGAG
    GGCTTCTTCA AACCAGAGTA TCAGGCAAGG CTGCCCCAAG CTACAGGGGA GACCTTTTCC
    ATGTGTATCC CACCTCCCAA TGTCACCGGC TCCCTGCACA TTGGCCATGC ACTCACAGTG
    GCGATCCAGG ACGCACTTGT GCGCTGGCAC CGGATGCGTG GGGATCGCGT GCTGTGGATC
    CCCGGGTCTG ATCACGCAGG AATTGCTACA CAGGCCATGG TGGAGAAGCA GCTATGGAAG
    GAGCAGAGGG TGCGGAGACA TGAGCTGAGC CGGGAAGACT TCCTCAGGGC CGTGTGGCAG
    TGGAAACATG AGAAAGGAGG GGAGATCTAT GAACAGCTGT GTGCTCTGGG TGCCTCTCTG
    GACTGGGATC GAGAGTGTTT CACCATGGAT GCCGGCTCCT CAGCAGCTGT GACGGAAGCT
    TTCGTGCGAC TCTACAACTC GGGATTGTTG TACCGGAACC GCCAGCTTGT CAACTGGTCA
    TGTACTTTGC GATCGGCCAT CTCAGACATT GAGGTGGAAA GCCGGCCTCT GCCTGGCCGC
    ACGGTGCTGC AGCTGCCCGG ATGCCCAACC CCTGTGTCTT TTGGGCTCCT TGCTTCTGTT
    GCTTTCCCTG TGGATGGAGA GCCTGACACA GAGATTGTGG TGGGAACCAC CAGGCCAGAG
    ACCCTGCCTG GAGATGTGGC TGTGGCCGTT CATCCAGATG ACCCGAGATA CACGCATTTA
    CATGGGCGAC AGCTCCGTCA TCCTTTGACA GGACAGCTTC TCCCCCTCAT CACAGACACC
    ACTGTTCAGC CACATGTGGG CACAGGGGCA GTGAAGGTGA CTCCTGCTCA CAGTCCAATA
    GATGCTGAGA TAGGGACCCG GCATGGCCTG ACCCCACTGA GTGTCATTGC AGAGGATGGG
    ACCATGACAT CCCTCTGTGG AGACTGGCTG CAGGGCCTTC ATCGATTTGT GGCCCGGGAA
    AAGATCATGT GCACACTGAG GGAGCAGGGC CTATTCCGGG GCCTACAGGA GCACCCCATG
    GTACTGCCCA TCTGCAGCCG TTCCGGGGAC GTGGTGGAGT ACCTGCTGAA GAGCCAGTGG
    TTTGTCCGCT GCCAGGAAAT GGGGGACCTA GCTGCCAAGG CTGTGGAGTC AGGGGCTCTG
    GAGCTCTGGC CCTCCTTCCA CCAGAAGAGC TGGCAACACT GGTTTGCCCA CATTGGGGAC
    TGGTGTGTCT CCCGGCAGCT GTGGTGGGGC CATCAGATTC CAGCCTACCG GGTCATCGGG
    GAGAATGCAG AGGATGACAG GAAGGAATGT TGGGTGGTCG GACGGTCAGA GGCTGAGGCC
    AGAGCGGTAG CAGCAAAACG GACAGGGAGA CCAGAGGCAG AGCTGACCCT GGAGAGGGAC
    CCTGACGTCC TGGACACTTG GTTCTCTTCG GCTCTTTTCC CCTTTTCTGC CCTGGGTTGG
    CCGCGAGAGA CGCCAGACCT TGCTCACTTC TACCCCCTCA CCCTCTTGGA AACGGGTAGT
    GACCTGCTGA TGTTCTGGGT GGGCCGCATG GTCATGCTGG GGACCCAGCT CACTGGGCAG
    CTCCCCTTCA GCAAGGTGCT TCTTCACTCC ATGGTTCGGG ACAGGCAGGG CCGGAAGATG
    AGCAAGTCCC TGGGGAACGT GCTAGACCCA CGGGACATCA TTAGCGGGCA GGAGCTGCAG
    GTGCTGCAGG CCAAGCTGAG AGACGGTAAC TTGGACCCTG GAGAGCTCGC CGTTGCAGCC
    GCAGCACAGA AAAAGGACTT TCCTTACGGG ATCCCTGAGT GTGGGACGGA TGCCCTGAGA
    TTTGCACTGT GCTCCCATGG AATCCTGGGG GGCGACCTGC ACCTGTCTGT CTCTGAGGTC
    CTGAACTACC GCCATTTCTG CAACAAGCTC TGGAATGCCC TGCGCTTTGT CCTCCGTGCC
    CTGGGGGATA ACTTCGTGCC CCAACCAGCA GAGGAGGTAA CCCCCTCCTC TCCCATGGAT
    GCCTGGATTC TAAGCCGCCT GGCCTTTGCA GCCAGCGAAT GTGAGAGAGG CTTCCTGAGT
    CGGGAGCTTT CGCTTGTCAC CCACACCCTG TATCATTTCT GGCTCCACAA TCTCTGTGAT
    GTCTACCTGG AAGCAGTGAA GCCAGTGTTG TCGAGCGTGC CCCGTCCCCC AGGACCCCCT
    CAGGTCCTGT TTTCCTGTGC AGACGTGGGT CTACGCCTCC TTGCCCCGCT GATGCCCTTC
    CTGGCCGAGG AGCTGTGGCA GAGGCTGCCC CCCAGGCCAG GCGGCCCCCT TGCCCCCAGC
    ATCTGTGTGG CCCCCTACCC CAGCACCCGC AGCCTGGAGT TCTGGCGCCA GCCAGAGCTG
    GAGCGCTGCT TCTCCAGAGT CCAAGAGGTT GTACAGGCAT TGCGGGCGCT CCGAGCCACC
    TACCAGCTCA CAAAAGCCCG ACCCCAAGTG CTGCTGCAGT GCTCGGACCC AGGAGAACAA
    GGCCTAGTCC AGCCCTTTCT GGAGCCTCTG GGCATCCTGA GCCACTGCGG GGCTGTAGGA
    TTCCTGTCCC CAGGGGCAGC AGCTCCCTCA GGGTGGGCCC TGACCCCACT GGGTGACACC
    ATGAAGATCT ACATGGAGCT GCAGGGCCTG GTGGACCCCC AGAGCCAGCT GCCCCGGCTC
    ACTGCCCGAA GGCAGAAGTT ACAGAAGCAG CTTGATGACC TGTTGAACCG GACCATGTCA
    GAGGGTCTTG CAGAGAGGCA GCAGAGGATT TCCTCCCTCC ATCTGGAATT GTCAAAGCTG
    GACCAGGCAG CCTCCTACCT ACAGCAACTG ATGGATGAGG CTCCCAGTGC CAGGGAGCTC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.