LIG1 cDNA ORF clone, Bos taurus(cattle)

The following LIG1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LIG1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa40221 NM_001102548.1
Latest version!
Bos taurus DNA ligase 1 (LIG1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa28788 XM_005219131.3
Latest version!
Bos taurus ligase I, DNA, ATP-dependent (LIG1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa58683 XM_010814980.2
Latest version!
Bos taurus ligase I, DNA, ATP-dependent (LIG1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa58684 XM_010814981.2
Latest version!
Bos taurus ligase I, DNA, ATP-dependent (LIG1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa58685 XM_010814982.2
Latest version!
Bos taurus ligase I, DNA, ATP-dependent (LIG1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa224759 XM_010814980.3
Latest version!
Bos taurus DNA ligase 1 (LIG1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa224760 XM_010814981.3
Latest version!
Bos taurus DNA ligase 1 (LIG1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa224761 XM_005219131.4
Latest version!
Bos taurus DNA ligase 1 (LIG1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa224762 XM_010814982.3
Latest version!
Bos taurus DNA ligase 1 (LIG1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa40221
    Clone ID Related Accession (Same CDS sequence) NM_001102548.1
    Accession Version NM_001102548.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2751bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-13
    Organism Bos taurus(cattle)
    Product DNA ligase 1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC134518.1. ##Evidence-Data-START## Transcript exon combination :: BC134518.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCAGCGAA GTATCATGTC ATTTTTCCAG CCCAAGAAAG AGGGCAAAGC GAAGAAGCCA 
    GAGAAGGAGA CAGCCAAGAG CATCAGAGAA ACAGAGTCCC CTCCGAAGGT GGCGCTGAAG
    GAGCGGAATC GAGTGGTGTC TGAGGGTGAC TCTCCAGTGA AGAGGCCAGG GAGGAAGGCG
    ACCCGGGTCC TGGGCAGCGA GGGCGAGGAG GAGGAAGAGG AAGCCCCCAC CACCCCCAGA
    AGCCAGAAGC CTGCTTCAGA CTCTCCACAG GCCTCGCCTC CCAGTCCTGT CACACTGCCT
    GAGAGCAGCC CTTCCCACTC CAAACCCTCC CCAGCAGTTG CATCCCCATC AGGGATCCCG
    AAGCGTCGCA CGGCCCGGAA GCAGCTTCCC AAACGGACAT TTCAAGATGT TCTGAAAGAG
    CAGGGCGAGG ACAAGGATCC AGAAACTAAG AAGAAGAAGG AGGAAGAAGC GGAGACACCA
    ATAGAAAGCC TCCCAGAGCC TGAAGGGGAC GACAAGGAGG AAGTGGAAGG TGGGGACCAG
    CCCACGACGC CCCCTGAGCC CCGGAAGACC TCCACAGAGT CCCTGACAGA AAGCGTCTCA
    GAGCCCGGGG TGACGGTGAA GCAGGAACCA CAGGAGGATG GCCAGGCTCA ACCGCCCCCC
    AAAACTCCAA AGACCCTCAG CAGTTTCTTC TCCCCCCGGA AGCCAGCTGT CAAAAAAGAA
    GCGAAAGAAG AAGGACCTGG TGCTCTGAGA AAGGACGAGA TCAAGGGACA CGTGGACCCA
    GCGAGCTATA ATCCTGCCAA GAACAACTAT CACCCCATCG AAGATGCCTG CTGGAAGGCG
    GGCCAGAGGG TTCCTTATCT GGCTGTGGCC CGGGCGTTCG AGAAGATCGA GGAGGTCTCT
    GCTCGGCTCC GGATGGTGGA GACGCTGAGC AACCTGCTGC GCTCCGTGGT GGCCCTGTCA
    CCTGCTGACC TCCTGCCCGT CCTCTACCTG AGCCTCAACC GCCTGGGGCC GCCCCAGCAG
    GGCCTGGAGC TCGGCATCGG AGAGGGCATC CTCCTCAAGG CAGTGGCCCA GGCCACGGGT
    CGGCAGCTGG AGTCCGTCCG GGCCGAGGTG GCCGAGAAGG GTGATGTGGG GCTGGTGGCC
    GAGAGCAGCC GCAGCACCCA GAGGCTCGTG CTGCCCCCGC CTGCGCTCAC TGCCGCCGGC
    GTCTTCGCCA AGTTCCGGGA CATCGCGCAG CTGGCAGGCA GCGCTTCCAC AACCAAGAAG
    ATTGACGTCA TCAAAGGCCT CTTTGTGGCC TGCCGTCACT CAGAGGCACG TTTCATTGCC
    AGAGCCCTGA GTGGACGGCT GCGCCTCGGG TTGGCTGAGC AGTCGGTGCT GGCGGCCCTC
    GCCCAGGCCG TGAGCCTCAC GCCCCCCAGT CAAGAATGCC CCCCCGCCGT GGTGGACGCC
    GGGAAGGGCA AGACGGCAGA AGCCAGAAAG ACGTGGCTGG AGGAGCAAGG CATGATCCTG
    AAGCAGACTT TCTGTGAGGT ACCTGACCTG GAGCGGATCG TCCCCGTGCT TCTGGAACAC
    GGCCTGGAGC GGCTCCCGGA GCACTGCAGA CTGAGCCCAG GGGTTCCCCT GAAACCAATG
    TTGGCCCACC CCACCCGGGG TGTCAGTGAG GTCCTGAAGC GCTTTGAGGA GGCAGCTTTC
    ACCTGCGAGT ACAAATACGA CGGGCAGAGG GCACAGATCC ACGTTCTGGA AGGCGGGGAG
    GTAAAGATCT TCAGCAGGAA TCAGGAGGAC AATACTGGAA GGTACCCCGA CATTATCAGC
    CGCATCCCTA AGATTAAACT CCCATCGGTC ACATCCTTCA TCCTAGACAC AGAAGCTGTA
    GCTTGGGACC GGGAAAAGAA GCAGATCCAG CCATTCCAAG TCCTCACCAC CCGCAAACGC
    AAGGAGGTGG ACGCGGCGGA GATCCAGGTG CAGGTGTGTC TGTATGCCTT CGATCTCATT
    TACCTCAACG GAAAGTCCCT GGTACGTGAG CCCCTCTCCC GACGCCGGCA GCTGCTCCGG
    GAGAACTTCG TGGAGACGGA GGGCGAGTTC GTCTTTGCCA CCTCCCTAGA CACCAAGGAC
    ACGGAGCAGA TCGCAGAGTT CTTGGAGCAG TCGGTGAAAG ACTCCTGCGA GGGGCTGATG
    GTGAAGACCC TGGACGTGGA CGCCACCTAC GAGATCGCCA AGAGGTCACA CAACTGGCTC
    AAGCTGAAGA AGGACTACCT CGAGGGTGTG GGCGACACCC TGGACCTCGT AGTGATTGGT
    GCCTACCTGG GCAAGGGGAA ACGGGCCGGC CGGTACGGGG GCTTCCTGCT GGCCTCCTAT
    GACGAGGAGA GCGAGGAGTT CCAGGCCATC TGCAAGCTCG GAACTGGCTT CAGTGACGAG
    GAGCTGGAAG AGCACCACCA GAGGCTCCAG GCCTTAGTGC TGCCCACCCC GCGCTCCTAC
    GTTCGGGCTG ACGGTGCGGT GGCCCCCGAC CACTGGCTGG ACCCCAGCGA CGTGTGGGAG
    GTGAAGTGCG CGGACCTCTC CCTGTCCCCC ATCTACCCTG CGGCCCGAGG CCTGGTGGAC
    AGCGAGAAGG GGATCTCCCT CCGCTTCCCT CGGTTTATTC GCGTCCGTGA GGATAAGAAG
    CCGGAGCAGG CCACTACCAG TGCCCAGGTG GCCGGTCTGT ACAAGAAGCA GAGTCAGATT
    CAGAACCAGC AGGGTGCGGA GCCGGACTCC GAGCAGGAAG AGTTCTACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001096018.1
    CDS122..2872
    Translation

    Target ORF information:

    RefSeq Version NM_001102548.1
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA ligase 1 (LIG1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001102548.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCAGCGAA GTATCATGTC ATTTTTCCAG CCCAAGAAAG AGGGCAAAGC GAAGAAGCCA 
    GAGAAGGAGA CAGCCAAGAG CATCAGAGAA ACAGAGTCCC CTCCGAAGGT GGCGCTGAAG
    GAGCGGAATC GAGTGGTGTC TGAGGGTGAC TCTCCAGTGA AGAGGCCAGG GAGGAAGGCG
    ACCCGGGTCC TGGGCAGCGA GGGCGAGGAG GAGGAAGAGG AAGCCCCCAC CACCCCCAGA
    AGCCAGAAGC CTGCTTCAGA CTCTCCACAG GCCTCGCCTC CCAGTCCTGT CACACTGCCT
    GAGAGCAGCC CTTCCCACTC CAAACCCTCC CCAGCAGTTG CATCCCCATC AGGGATCCCG
    AAGCGTCGCA CGGCCCGGAA GCAGCTTCCC AAACGGACAT TTCAAGATGT TCTGAAAGAG
    CAGGGCGAGG ACAAGGATCC AGAAACTAAG AAGAAGAAGG AGGAAGAAGC GGAGACACCA
    ATAGAAAGCC TCCCAGAGCC TGAAGGGGAC GACAAGGAGG AAGTGGAAGG TGGGGACCAG
    CCCACGACGC CCCCTGAGCC CCGGAAGACC TCCACAGAGT CCCTGACAGA AAGCGTCTCA
    GAGCCCGGGG TGACGGTGAA GCAGGAACCA CAGGAGGATG GCCAGGCTCA ACCGCCCCCC
    AAAACTCCAA AGACCCTCAG CAGTTTCTTC TCCCCCCGGA AGCCAGCTGT CAAAAAAGAA
    GCGAAAGAAG AAGGACCTGG TGCTCTGAGA AAGGACGAGA TCAAGGGACA CGTGGACCCA
    GCGAGCTATA ATCCTGCCAA GAACAACTAT CACCCCATCG AAGATGCCTG CTGGAAGGCG
    GGCCAGAGGG TTCCTTATCT GGCTGTGGCC CGGGCGTTCG AGAAGATCGA GGAGGTCTCT
    GCTCGGCTCC GGATGGTGGA GACGCTGAGC AACCTGCTGC GCTCCGTGGT GGCCCTGTCA
    CCTGCTGACC TCCTGCCCGT CCTCTACCTG AGCCTCAACC GCCTGGGGCC GCCCCAGCAG
    GGCCTGGAGC TCGGCATCGG AGAGGGCATC CTCCTCAAGG CAGTGGCCCA GGCCACGGGT
    CGGCAGCTGG AGTCCGTCCG GGCCGAGGTG GCCGAGAAGG GTGATGTGGG GCTGGTGGCC
    GAGAGCAGCC GCAGCACCCA GAGGCTCGTG CTGCCCCCGC CTGCGCTCAC TGCCGCCGGC
    GTCTTCGCCA AGTTCCGGGA CATCGCGCAG CTGGCAGGCA GCGCTTCCAC AACCAAGAAG
    ATTGACGTCA TCAAAGGCCT CTTTGTGGCC TGCCGTCACT CAGAGGCACG TTTCATTGCC
    AGAGCCCTGA GTGGACGGCT GCGCCTCGGG TTGGCTGAGC AGTCGGTGCT GGCGGCCCTC
    GCCCAGGCCG TGAGCCTCAC GCCCCCCAGT CAAGAATGCC CCCCCGCCGT GGTGGACGCC
    GGGAAGGGCA AGACGGCAGA AGCCAGAAAG ACGTGGCTGG AGGAGCAAGG CATGATCCTG
    AAGCAGACTT TCTGTGAGGT ACCTGACCTG GAGCGGATCG TCCCCGTGCT TCTGGAACAC
    GGCCTGGAGC GGCTCCCGGA GCACTGCAGA CTGAGCCCAG GGGTTCCCCT GAAACCAATG
    TTGGCCCACC CCACCCGGGG TGTCAGTGAG GTCCTGAAGC GCTTTGAGGA GGCAGCTTTC
    ACCTGCGAGT ACAAATACGA CGGGCAGAGG GCACAGATCC ACGTTCTGGA AGGCGGGGAG
    GTAAAGATCT TCAGCAGGAA TCAGGAGGAC AATACTGGAA GGTACCCCGA CATTATCAGC
    CGCATCCCTA AGATTAAACT CCCATCGGTC ACATCCTTCA TCCTAGACAC AGAAGCTGTA
    GCTTGGGACC GGGAAAAGAA GCAGATCCAG CCATTCCAAG TCCTCACCAC CCGCAAACGC
    AAGGAGGTGG ACGCGGCGGA GATCCAGGTG CAGGTGTGTC TGTATGCCTT CGATCTCATT
    TACCTCAACG GAAAGTCCCT GGTACGTGAG CCCCTCTCCC GACGCCGGCA GCTGCTCCGG
    GAGAACTTCG TGGAGACGGA GGGCGAGTTC GTCTTTGCCA CCTCCCTAGA CACCAAGGAC
    ACGGAGCAGA TCGCAGAGTT CTTGGAGCAG TCGGTGAAAG ACTCCTGCGA GGGGCTGATG
    GTGAAGACCC TGGACGTGGA CGCCACCTAC GAGATCGCCA AGAGGTCACA CAACTGGCTC
    AAGCTGAAGA AGGACTACCT CGAGGGTGTG GGCGACACCC TGGACCTCGT AGTGATTGGT
    GCCTACCTGG GCAAGGGGAA ACGGGCCGGC CGGTACGGGG GCTTCCTGCT GGCCTCCTAT
    GACGAGGAGA GCGAGGAGTT CCAGGCCATC TGCAAGCTCG GAACTGGCTT CAGTGACGAG
    GAGCTGGAAG AGCACCACCA GAGGCTCCAG GCCTTAGTGC TGCCCACCCC GCGCTCCTAC
    GTTCGGGCTG ACGGTGCGGT GGCCCCCGAC CACTGGCTGG ACCCCAGCGA CGTGTGGGAG
    GTGAAGTGCG CGGACCTCTC CCTGTCCCCC ATCTACCCTG CGGCCCGAGG CCTGGTGGAC
    AGCGAGAAGG GGATCTCCCT CCGCTTCCCT CGGTTTATTC GCGTCCGTGA GGATAAGAAG
    CCGGAGCAGG CCACTACCAG TGCCCAGGTG GCCGGTCTGT ACAAGAAGCA GAGTCAGATT
    CAGAACCAGC AGGGTGCGGA GCCGGACTCC GAGCAGGAAG AGTTCTACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa28788
    Clone ID Related Accession (Same CDS sequence) XM_005219131.3
    Accession Version XM_005219131.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2748bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA ligase 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104466.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005219131.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCAGCGAA GTATCATGTC ATTTTTCCAG CCCAAGAAAG AGGGCAAAGC GAAGAAGCCA 
    GAGAAGGAGA CAGCCAAGAG CATCAGAGAA ACAGAGTCCC CTCCGAAGGT GGCGCTGAAG
    GAGCGGAATC GAGTGGTGTC TGAGGGTGAC TCTCCAGTGA AGAGGCCAGG GAGGAAGGCG
    ACCCGGGTCC TGGGCAGCGA GGGCGAGGAG GAGGAAGAGG AAGCCCCCAC CACCCCCAGA
    AGCCAGAAGC CTGCTTCAGA CTCTCCACAG GCCTCGCCTC CCAGTCCTGT CACACTGCCT
    GAGAGCAGCC CTTCCCACTC CAAACCCTCC CCAGCAGTTG CATCCCCATC AGGGATCCCG
    AAGCGTCGCA CGGCCCGGAA GCAGCTTCCC AAACGGACAT TTCAAGATGT TCTGAAAGAG
    CAGGGCGAGG ACAAGGATCC AGAAACTAAG AAGAAGAAGG AGGAAGAAGC GGAGACACCA
    ATAGAAAGCC TCCCAGAGCC TGAAGGGGAC GACAAGGAGG AAGTGGAAGG TGGGGACCAG
    CCCACGACGC CCCCTGAGCC CCGGAAGACC TCCAAGTCCC TGACAGAAAG CGTCTCAGAG
    CCCGGGGTGA CGGTGAAGCA GGAACCACAG GAGGATGGCC AGGCTCAACC GCCCCCCAAA
    ACTCCAAAGA CCCTCAGCAG TTTCTTCTCC CCCCGGAAGC CAGCTGTCAA AAAAGAAGCG
    AAAGAAGAAG GACCTGGTGC TCTGAGAAAG GACGAGATCA AGGGACACGT GGACCCAGCG
    AGCTATAATC CTGCCAAGAA CAACTATCAC CCCATCGAAG ATGCCTGCTG GAAGGCGGGC
    CAGAGGGTTC CTTATCTGGC TGTGGCCCGG GCGTTCGAGA AGATCGAGGA GGTCTCTGCT
    CGGCTCCGGA TGGTGGAGAC GCTGAGCAAC CTGCTGCGCT CCGTGGTGGC CCTGTCACCT
    GCTGACCTCC TGCCCGTCCT CTACCTGAGC CTCAACCGCC TGGGGCCGCC CCAGCAGGGC
    CTGGAGCTCG GCATCGGAGA GGGCATCCTC CTCAAGGCAG TGGCCCAGGC CACGGGTCGG
    CAGCTGGAGT CCGTCCGGGC CGAGGTGGCC GAGAAGGGTG ATGTGGGGCT GGTGGCCGAG
    AGCAGCCGCA GCACCCAGAG GCTCGTGCTG CCCCCGCCTG CGCTCACTGC CGCCGGCGTC
    TTCGCCAAGT TCCGGGACAT CGCGCAGCTG GCAGGCAGCG CTTCCACAAC CAAGAAGATT
    GACGTCATCA AAGGCCTCTT TGTGGCCTGC CGTCACTCAG AGGCACGTTT CATTGCCAGA
    GCCCTGAGTG GACGGCTGCG CCTCGGGTTG GCTGAGCAGT CGGTGCTGGC GGCCCTCGCC
    CAGGCCGTGA GCCTCACGCC CCCCAGTCAA GAATGCCCCC CCGCCGTGGT GGACGCCGGG
    AAGGGCAAGA CGGCAGAAGC CAGAAAGACG TGGCTGGAGG AGCAAGGCAT GATCCTGAAG
    CAGACTTTCT GTGAGGTACC TGACCTGGAG CGGATCGTCC CCGTGCTTCT GGAACACGGC
    CTGGAGCGGC TCCCGGAGCA CTGCAGACTG AGCCCAGGGG TTCCCCTGAA ACCAATGTTG
    GCCCACCCCA CCCGGGGTGT CAGTGAGGTC CTGAAGCGCT TTGAGGAGGC AGCTTTCACC
    TGCGAGTACA AATACGACGG GCAGAGGGCA CAGATCCACG TTCTGGAAGG CGGGGAGGTA
    AAGATCTTCA GCAGGAATCA GGAGGACAAT ACTGGAAGGT ACCCCGACAT TATCAGCCGC
    ATCCCTAAGA TTAAACTCCC ATCGGTCACA TCCTTCATCC TAGACACAGA AGCTGTAGCT
    TGGGACCGGG AAAAGAAGCA GATCCAGCCA TTCCAAGTCC TCACCACCCG CAAACGCAAG
    GAGGTGGACG CGGCGGAGAT CCAGGTGCAG GTGTGTCTGT ATGCCTTCGA TCTCATTTAC
    CTCAACGGAA AGTCCCTGGT ACGTGAGCCC CTCTCCCGAC GCCGGCAGCT GCTCCGGGAG
    AACTTCGTGG AGACGGAGGG CGAGTTCGTC TTTGCCACCT CCCTAGACAC CAAGGACACG
    GAGCAGATCG CAGAGTTCTT GGAGCAGTCG GTGAAAGACT CCTGCGAGGG GCTGATGGTG
    AAGACCCTGG ACGTGGACGC CACCTACGAG ATCGCCAAGA GGTCACACAA CTGGCTCAAG
    CTGAAGAAGG ACTACCTCGA GGGTGTGGGC GACACCCTGG ACCTCGTAGT GATTGGTGCC
    TACCTGGGCA AGGGGAAACG GGCCGGCCGG TACGGGGGCT TCCTGCTGGC CTCCTATGAC
    GAGGAGAGCG AGGAGTTCCA GGCCATCTGC AAGCTCGGAA CTGGCTTCAG TGACGAGGAG
    CTGGAAGAGC ACCACCAGAG GCTCCAGGCC TTAGTGCTGC CCACCCCGCG CTCCTACGTT
    CGGGCTGACG GTGCGGTGGC CCCCGACCAC TGGCTGGACC CCAGCGACGT GTGGGAGGTG
    AAGTGCGCGG ACCTCTCCCT GTCCCCCATC TACCCTGCGG CCCGAGGCCT GGTGGACAGC
    GAGAAGGGGA TCTCCCTCCG CTTCCCTCGG TTTATTCGCG TCCGTGAGGA TAAGAAGCCG
    GAGCAGGCCA CTACCAGTGC CCAGGTGGCC GGTCTGTACA AGAAGCAGAG TCAGATTCAG
    AACCAGCAGG GTGCGGAGCC GGACTCCGAG CAGGAAGAGT TCTACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005219188.1
    CDS172..2919
    Translation

    Target ORF information:

    RefSeq Version XM_005219131.3
    Organism Bos taurus(cattle)
    Definition Bos taurus ligase I, DNA, ATP-dependent (LIG1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005219131.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCAGCGAA GTATCATGTC ATTTTTCCAG CCCAAGAAAG AGGGCAAAGC GAAGAAGCCA 
    GAGAAGGAGA CAGCCAAGAG CATCAGAGAA ACAGAGTCCC CTCCGAAGGT GGCGCTGAAG
    GAGCGGAATC GAGTGGTGTC TGAGGGTGAC TCTCCAGTGA AGAGGCCAGG GAGGAAGGCG
    ACCCGGGTCC TGGGCAGCGA GGGCGAGGAG GAGGAAGAGG AAGCCCCCAC CACCCCCAGA
    AGCCAGAAGC CTGCTTCAGA CTCTCCACAG GCCTCGCCTC CCAGTCCTGT CACACTGCCT
    GAGAGCAGCC CTTCCCACTC CAAACCCTCC CCAGCAGTTG CATCCCCATC AGGGATCCCG
    AAGCGTCGCA CGGCCCGGAA GCAGCTTCCC AAACGGACAT TTCAAGATGT TCTGAAAGAG
    CAGGGCGAGG ACAAGGATCC AGAAACTAAG AAGAAGAAGG AGGAAGAAGC GGAGACACCA
    ATAGAAAGCC TCCCAGAGCC TGAAGGGGAC GACAAGGAGG AAGTGGAAGG TGGGGACCAG
    CCCACGACGC CCCCTGAGCC CCGGAAGACC TCCAAGTCCC TGACAGAAAG CGTCTCAGAG
    CCCGGGGTGA CGGTGAAGCA GGAACCACAG GAGGATGGCC AGGCTCAACC GCCCCCCAAA
    ACTCCAAAGA CCCTCAGCAG TTTCTTCTCC CCCCGGAAGC CAGCTGTCAA AAAAGAAGCG
    AAAGAAGAAG GACCTGGTGC TCTGAGAAAG GACGAGATCA AGGGACACGT GGACCCAGCG
    AGCTATAATC CTGCCAAGAA CAACTATCAC CCCATCGAAG ATGCCTGCTG GAAGGCGGGC
    CAGAGGGTTC CTTATCTGGC TGTGGCCCGG GCGTTCGAGA AGATCGAGGA GGTCTCTGCT
    CGGCTCCGGA TGGTGGAGAC GCTGAGCAAC CTGCTGCGCT CCGTGGTGGC CCTGTCACCT
    GCTGACCTCC TGCCCGTCCT CTACCTGAGC CTCAACCGCC TGGGGCCGCC CCAGCAGGGC
    CTGGAGCTCG GCATCGGAGA GGGCATCCTC CTCAAGGCAG TGGCCCAGGC CACGGGTCGG
    CAGCTGGAGT CCGTCCGGGC CGAGGTGGCC GAGAAGGGTG ATGTGGGGCT GGTGGCCGAG
    AGCAGCCGCA GCACCCAGAG GCTCGTGCTG CCCCCGCCTG CGCTCACTGC CGCCGGCGTC
    TTCGCCAAGT TCCGGGACAT CGCGCAGCTG GCAGGCAGCG CTTCCACAAC CAAGAAGATT
    GACGTCATCA AAGGCCTCTT TGTGGCCTGC CGTCACTCAG AGGCACGTTT CATTGCCAGA
    GCCCTGAGTG GACGGCTGCG CCTCGGGTTG GCTGAGCAGT CGGTGCTGGC GGCCCTCGCC
    CAGGCCGTGA GCCTCACGCC CCCCAGTCAA GAATGCCCCC CCGCCGTGGT GGACGCCGGG
    AAGGGCAAGA CGGCAGAAGC CAGAAAGACG TGGCTGGAGG AGCAAGGCAT GATCCTGAAG
    CAGACTTTCT GTGAGGTACC TGACCTGGAG CGGATCGTCC CCGTGCTTCT GGAACACGGC
    CTGGAGCGGC TCCCGGAGCA CTGCAGACTG AGCCCAGGGG TTCCCCTGAA ACCAATGTTG
    GCCCACCCCA CCCGGGGTGT CAGTGAGGTC CTGAAGCGCT TTGAGGAGGC AGCTTTCACC
    TGCGAGTACA AATACGACGG GCAGAGGGCA CAGATCCACG TTCTGGAAGG CGGGGAGGTA
    AAGATCTTCA GCAGGAATCA GGAGGACAAT ACTGGAAGGT ACCCCGACAT TATCAGCCGC
    ATCCCTAAGA TTAAACTCCC ATCGGTCACA TCCTTCATCC TAGACACAGA AGCTGTAGCT
    TGGGACCGGG AAAAGAAGCA GATCCAGCCA TTCCAAGTCC TCACCACCCG CAAACGCAAG
    GAGGTGGACG CGGCGGAGAT CCAGGTGCAG GTGTGTCTGT ATGCCTTCGA TCTCATTTAC
    CTCAACGGAA AGTCCCTGGT ACGTGAGCCC CTCTCCCGAC GCCGGCAGCT GCTCCGGGAG
    AACTTCGTGG AGACGGAGGG CGAGTTCGTC TTTGCCACCT CCCTAGACAC CAAGGACACG
    GAGCAGATCG CAGAGTTCTT GGAGCAGTCG GTGAAAGACT CCTGCGAGGG GCTGATGGTG
    AAGACCCTGG ACGTGGACGC CACCTACGAG ATCGCCAAGA GGTCACACAA CTGGCTCAAG
    CTGAAGAAGG ACTACCTCGA GGGTGTGGGC GACACCCTGG ACCTCGTAGT GATTGGTGCC
    TACCTGGGCA AGGGGAAACG GGCCGGCCGG TACGGGGGCT TCCTGCTGGC CTCCTATGAC
    GAGGAGAGCG AGGAGTTCCA GGCCATCTGC AAGCTCGGAA CTGGCTTCAG TGACGAGGAG
    CTGGAAGAGC ACCACCAGAG GCTCCAGGCC TTAGTGCTGC CCACCCCGCG CTCCTACGTT
    CGGGCTGACG GTGCGGTGGC CCCCGACCAC TGGCTGGACC CCAGCGACGT GTGGGAGGTG
    AAGTGCGCGG ACCTCTCCCT GTCCCCCATC TACCCTGCGG CCCGAGGCCT GGTGGACAGC
    GAGAAGGGGA TCTCCCTCCG CTTCCCTCGG TTTATTCGCG TCCGTGAGGA TAAGAAGCCG
    GAGCAGGCCA CTACCAGTGC CCAGGTGGCC GGTCTGTACA AGAAGCAGAG TCAGATTCAG
    AACCAGCAGG GTGCGGAGCC GGACTCCGAG CAGGAAGAGT TCTACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa58683
    Clone ID Related Accession (Same CDS sequence) XM_010814980.2
    Accession Version XM_010814980.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2796bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA ligase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104466.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010814980.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGCAGCGAA GTATCATGTC ATTTTTCCAG CCCAAGAAAG AGGGCAAAGC GAAGAAGCCA 
    GAGAAGGAGA CAGCCAAGAG CATCAGAGAA ACAGAGTCCC CTCCGAAGGT GGCGCTGAAG
    GAGCGGAATC GAGTGGTGTC TGAGGGTGAC TCTCCAGTGA AGAGGCCAGG GAGGAAGGCG
    ACCCGGGTCC TGGGCAGCGA GGGCGAGGAG GAGGAAGAGG AAGCCCCCAC CACCCCCAGA
    AGCCAGAAGC CTGCTTCAGA CTCTCCACAG GCCTCGCCTC CCAGTCCTGT CACACTGCCT
    GAGAGCAGCC CTTCCCACTC CAAACCCTCC CCAGCAGTTG CATCCCCATC AGGGATCCCG
    AAGCGTCGCA CGGCCCGGAA GCAGCTTCCC AAACGGACAT TTCAAGATGT TCTGAAAGAG
    CAGGGCGAGG ACAAGGATCC AGAAACTAAG AAGAAGAAGG AGGAAGAAGC GGAGACACCA
    ATAGAAAGCC TCCCAGAGCC TGAAGGGGAC GACAAGGAGG AAGTGGAAGG TGGGGACCAG
    CCCACGACGC CCCCTGAGCC CCGGAAGACC TCCACAGAGT CCCTGACAGA AAGCGTCTCA
    GAGCCCGGGG TGACGGTGAA GCAGGAACCA CAGGAGGATG GCCAGGCTCA ACCGCCCCCC
    AAAACTCCAA AGACCCTCAG CAGTTTCTTC TGTGAGTGGG GACTTCACCT TGCGGGGCTG
    CGCAGGGAAG AGGCAGCCCC CCGGAAGCCA GCTGTCAAAA AAGAAGCGAA AGAAGAAGGA
    CCTGGTGCTC TGAGAAAGGA CGAGATCAAG GGACACGTGG ACCCAGCGAG CTATAATCCT
    GCCAAGAACA ACTATCACCC CATCGAAGAT GCCTGCTGGA AGGCGGGCCA GAGGGTTCCT
    TATCTGGCTG TGGCCCGGGC GTTCGAGAAG ATCGAGGAGG TCTCTGCTCG GCTCCGGATG
    GTGGAGACGC TGAGCAACCT GCTGCGCTCC GTGGTGGCCC TGTCACCTGC TGACCTCCTG
    CCCGTCCTCT ACCTGAGCCT CAACCGCCTG GGGCCGCCCC AGCAGGGCCT GGAGCTCGGC
    ATCGGAGAGG GCATCCTCCT CAAGGCAGTG GCCCAGGCCA CGGGTCGGCA GCTGGAGTCC
    GTCCGGGCCG AGGTGGCCGA GAAGGGTGAT GTGGGGCTGG TGGCCGAGAG CAGCCGCAGC
    ACCCAGAGGC TCGTGCTGCC CCCGCCTGCG CTCACTGCCG CCGGCGTCTT CGCCAAGTTC
    CGGGACATCG CGCAGCTGGC AGGCAGCGCT TCCACAACCA AGAAGATTGA CGTCATCAAA
    GGCCTCTTTG TGGCCTGCCG TCACTCAGAG GCACGTTTCA TTGCCAGAGC CCTGAGTGGA
    CGGCTGCGCC TCGGGTTGGC TGAGCAGTCG GTGCTGGCGG CCCTCGCCCA GGCCGTGAGC
    CTCACGCCCC CCAGTCAAGA ATGCCCCCCC GCCGTGGTGG ACGCCGGGAA GGGCAAGACG
    GCAGAAGCCA GAAAGACGTG GCTGGAGGAG CAAGGCATGA TCCTGAAGCA GACTTTCTGT
    GAGGTACCTG ACCTGGAGCG GATCGTCCCC GTGCTTCTGG AACACGGCCT GGAGCGGCTC
    CCGGAGCACT GCAGACTGAG CCCAGGGGTT CCCCTGAAAC CAATGTTGGC CCACCCCACC
    CGGGGTGTCA GTGAGGTCCT GAAGCGCTTT GAGGAGGCAG CTTTCACCTG CGAGTACAAA
    TACGACGGGC AGAGGGCACA GATCCACGTT CTGGAAGGCG GGGAGGTAAA GATCTTCAGC
    AGGAATCAGG AGGACAATAC TGGAAGGTAC CCCGACATTA TCAGCCGCAT CCCTAAGATT
    AAACTCCCAT CGGTCACATC CTTCATCCTA GACACAGAAG CTGTAGCTTG GGACCGGGAA
    AAGAAGCAGA TCCAGCCATT CCAAGTCCTC ACCACCCGCA AACGCAAGGA GGTGGACGCG
    GCGGAGATCC AGGTGCAGGT GTGTCTGTAT GCCTTCGATC TCATTTACCT CAACGGAAAG
    TCCCTGGTAC GTGAGCCCCT CTCCCGACGC CGGCAGCTGC TCCGGGAGAA CTTCGTGGAG
    ACGGAGGGCG AGTTCGTCTT TGCCACCTCC CTAGACACCA AGGACACGGA GCAGATCGCA
    GAGTTCTTGG AGCAGTCGGT GAAAGACTCC TGCGAGGGGC TGATGGTGAA GACCCTGGAC
    GTGGACGCCA CCTACGAGAT CGCCAAGAGG TCACACAACT GGCTCAAGCT GAAGAAGGAC
    TACCTCGAGG GTGTGGGCGA CACCCTGGAC CTCGTAGTGA TTGGTGCCTA CCTGGGCAAG
    GGGAAACGGG CCGGCCGGTA CGGGGGCTTC CTGCTGGCCT CCTATGACGA GGAGAGCGAG
    GAGTTCCAGG CCATCTGCAA GCTCGGAACT GGCTTCAGTG ACGAGGAGCT GGAAGAGCAC
    CACCAGAGGC TCCAGGCCTT AGTGCTGCCC ACCCCGCGCT CCTACGTTCG GGCTGACGGT
    GCGGTGGCCC CCGACCACTG GCTGGACCCC AGCGACGTGT GGGAGGTGAA GTGCGCGGAC
    CTCTCCCTGT CCCCCATCTA CCCTGCGGCC CGAGGCCTGG TGGACAGCGA GAAGGGGATC
    TCCCTCCGCT TCCCTCGGTT TATTCGCGTC CGTGAGGATA AGAAGCCGGA GCAGGCCACT
    ACCAGTGCCC AGGTGGCCGG TCTGTACAAG AAGCAGAGTC AGATTCAGAA CCAGCAGGGT
    GCGGAGCCGG ACTCCGAGCA GGAAGAGTTC TACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010813282.1
    CDS172..2967
    Translation

    Target ORF information:

    RefSeq Version XM_010814980.2
    Organism Bos taurus(cattle)
    Definition Bos taurus ligase I, DNA, ATP-dependent (LIG1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010814980.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGCAGCGAA GTATCATGTC ATTTTTCCAG CCCAAGAAAG AGGGCAAAGC GAAGAAGCCA 
    GAGAAGGAGA CAGCCAAGAG CATCAGAGAA ACAGAGTCCC CTCCGAAGGT GGCGCTGAAG
    GAGCGGAATC GAGTGGTGTC TGAGGGTGAC TCTCCAGTGA AGAGGCCAGG GAGGAAGGCG
    ACCCGGGTCC TGGGCAGCGA GGGCGAGGAG GAGGAAGAGG AAGCCCCCAC CACCCCCAGA
    AGCCAGAAGC CTGCTTCAGA CTCTCCACAG GCCTCGCCTC CCAGTCCTGT CACACTGCCT
    GAGAGCAGCC CTTCCCACTC CAAACCCTCC CCAGCAGTTG CATCCCCATC AGGGATCCCG
    AAGCGTCGCA CGGCCCGGAA GCAGCTTCCC AAACGGACAT TTCAAGATGT TCTGAAAGAG
    CAGGGCGAGG ACAAGGATCC AGAAACTAAG AAGAAGAAGG AGGAAGAAGC GGAGACACCA
    ATAGAAAGCC TCCCAGAGCC TGAAGGGGAC GACAAGGAGG AAGTGGAAGG TGGGGACCAG
    CCCACGACGC CCCCTGAGCC CCGGAAGACC TCCACAGAGT CCCTGACAGA AAGCGTCTCA
    GAGCCCGGGG TGACGGTGAA GCAGGAACCA CAGGAGGATG GCCAGGCTCA ACCGCCCCCC
    AAAACTCCAA AGACCCTCAG CAGTTTCTTC TGTGAGTGGG GACTTCACCT TGCGGGGCTG
    CGCAGGGAAG AGGCAGCCCC CCGGAAGCCA GCTGTCAAAA AAGAAGCGAA AGAAGAAGGA
    CCTGGTGCTC TGAGAAAGGA CGAGATCAAG GGACACGTGG ACCCAGCGAG CTATAATCCT
    GCCAAGAACA ACTATCACCC CATCGAAGAT GCCTGCTGGA AGGCGGGCCA GAGGGTTCCT
    TATCTGGCTG TGGCCCGGGC GTTCGAGAAG ATCGAGGAGG TCTCTGCTCG GCTCCGGATG
    GTGGAGACGC TGAGCAACCT GCTGCGCTCC GTGGTGGCCC TGTCACCTGC TGACCTCCTG
    CCCGTCCTCT ACCTGAGCCT CAACCGCCTG GGGCCGCCCC AGCAGGGCCT GGAGCTCGGC
    ATCGGAGAGG GCATCCTCCT CAAGGCAGTG GCCCAGGCCA CGGGTCGGCA GCTGGAGTCC
    GTCCGGGCCG AGGTGGCCGA GAAGGGTGAT GTGGGGCTGG TGGCCGAGAG CAGCCGCAGC
    ACCCAGAGGC TCGTGCTGCC CCCGCCTGCG CTCACTGCCG CCGGCGTCTT CGCCAAGTTC
    CGGGACATCG CGCAGCTGGC AGGCAGCGCT TCCACAACCA AGAAGATTGA CGTCATCAAA
    GGCCTCTTTG TGGCCTGCCG TCACTCAGAG GCACGTTTCA TTGCCAGAGC CCTGAGTGGA
    CGGCTGCGCC TCGGGTTGGC TGAGCAGTCG GTGCTGGCGG CCCTCGCCCA GGCCGTGAGC
    CTCACGCCCC CCAGTCAAGA ATGCCCCCCC GCCGTGGTGG ACGCCGGGAA GGGCAAGACG
    GCAGAAGCCA GAAAGACGTG GCTGGAGGAG CAAGGCATGA TCCTGAAGCA GACTTTCTGT
    GAGGTACCTG ACCTGGAGCG GATCGTCCCC GTGCTTCTGG AACACGGCCT GGAGCGGCTC
    CCGGAGCACT GCAGACTGAG CCCAGGGGTT CCCCTGAAAC CAATGTTGGC CCACCCCACC
    CGGGGTGTCA GTGAGGTCCT GAAGCGCTTT GAGGAGGCAG CTTTCACCTG CGAGTACAAA
    TACGACGGGC AGAGGGCACA GATCCACGTT CTGGAAGGCG GGGAGGTAAA GATCTTCAGC
    AGGAATCAGG AGGACAATAC TGGAAGGTAC CCCGACATTA TCAGCCGCAT CCCTAAGATT
    AAACTCCCAT CGGTCACATC CTTCATCCTA GACACAGAAG CTGTAGCTTG GGACCGGGAA
    AAGAAGCAGA TCCAGCCATT CCAAGTCCTC ACCACCCGCA AACGCAAGGA GGTGGACGCG
    GCGGAGATCC AGGTGCAGGT GTGTCTGTAT GCCTTCGATC TCATTTACCT CAACGGAAAG
    TCCCTGGTAC GTGAGCCCCT CTCCCGACGC CGGCAGCTGC TCCGGGAGAA CTTCGTGGAG
    ACGGAGGGCG AGTTCGTCTT TGCCACCTCC CTAGACACCA AGGACACGGA GCAGATCGCA
    GAGTTCTTGG AGCAGTCGGT GAAAGACTCC TGCGAGGGGC TGATGGTGAA GACCCTGGAC
    GTGGACGCCA CCTACGAGAT CGCCAAGAGG TCACACAACT GGCTCAAGCT GAAGAAGGAC
    TACCTCGAGG GTGTGGGCGA CACCCTGGAC CTCGTAGTGA TTGGTGCCTA CCTGGGCAAG
    GGGAAACGGG CCGGCCGGTA CGGGGGCTTC CTGCTGGCCT CCTATGACGA GGAGAGCGAG
    GAGTTCCAGG CCATCTGCAA GCTCGGAACT GGCTTCAGTG ACGAGGAGCT GGAAGAGCAC
    CACCAGAGGC TCCAGGCCTT AGTGCTGCCC ACCCCGCGCT CCTACGTTCG GGCTGACGGT
    GCGGTGGCCC CCGACCACTG GCTGGACCCC AGCGACGTGT GGGAGGTGAA GTGCGCGGAC
    CTCTCCCTGT CCCCCATCTA CCCTGCGGCC CGAGGCCTGG TGGACAGCGA GAAGGGGATC
    TCCCTCCGCT TCCCTCGGTT TATTCGCGTC CGTGAGGATA AGAAGCCGGA GCAGGCCACT
    ACCAGTGCCC AGGTGGCCGG TCTGTACAAG AAGCAGAGTC AGATTCAGAA CCAGCAGGGT
    GCGGAGCCGG ACTCCGAGCA GGAAGAGTTC TACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa58684
    Clone ID Related Accession (Same CDS sequence) XM_010814981.2
    Accession Version XM_010814981.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2793bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA ligase 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104466.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010814981.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGCAGCGAA GTATCATGTC ATTTTTCCAG CCCAAGAAAG AGGGCAAAGC GAAGAAGCCA 
    GAGAAGGAGA CAGCCAAGAG CATCAGAGAA ACAGAGTCCC CTCCGAAGGT GGCGCTGAAG
    GAGCGGAATC GAGTGGTGTC TGAGGGTGAC TCTCCAGTGA AGAGGCCAGG GAGGAAGGCG
    ACCCGGGTCC TGGGCAGCGA GGGCGAGGAG GAGGAAGAGG AAGCCCCCAC CACCCCCAGA
    AGCCAGAAGC CTGCTTCAGA CTCTCCACAG GCCTCGCCTC CCAGTCCTGT CACACTGCCT
    GAGAGCAGCC CTTCCCACTC CAAACCCTCC CCAGCAGTTG CATCCCCATC AGGGATCCCG
    AAGCGTCGCA CGGCCCGGAA GCAGCTTCCC AAACGGACAT TTCAAGATGT TCTGAAAGAG
    CAGGGCGAGG ACAAGGATCC AGAAACTAAG AAGAAGAAGG AGGAAGAAGC GGAGACACCA
    ATAGAAAGCC TCCCAGAGCC TGAAGGGGAC GACAAGGAGG AAGTGGAAGG TGGGGACCAG
    CCCACGACGC CCCCTGAGCC CCGGAAGACC TCCAAGTCCC TGACAGAAAG CGTCTCAGAG
    CCCGGGGTGA CGGTGAAGCA GGAACCACAG GAGGATGGCC AGGCTCAACC GCCCCCCAAA
    ACTCCAAAGA CCCTCAGCAG TTTCTTCTGT GAGTGGGGAC TTCACCTTGC GGGGCTGCGC
    AGGGAAGAGG CAGCCCCCCG GAAGCCAGCT GTCAAAAAAG AAGCGAAAGA AGAAGGACCT
    GGTGCTCTGA GAAAGGACGA GATCAAGGGA CACGTGGACC CAGCGAGCTA TAATCCTGCC
    AAGAACAACT ATCACCCCAT CGAAGATGCC TGCTGGAAGG CGGGCCAGAG GGTTCCTTAT
    CTGGCTGTGG CCCGGGCGTT CGAGAAGATC GAGGAGGTCT CTGCTCGGCT CCGGATGGTG
    GAGACGCTGA GCAACCTGCT GCGCTCCGTG GTGGCCCTGT CACCTGCTGA CCTCCTGCCC
    GTCCTCTACC TGAGCCTCAA CCGCCTGGGG CCGCCCCAGC AGGGCCTGGA GCTCGGCATC
    GGAGAGGGCA TCCTCCTCAA GGCAGTGGCC CAGGCCACGG GTCGGCAGCT GGAGTCCGTC
    CGGGCCGAGG TGGCCGAGAA GGGTGATGTG GGGCTGGTGG CCGAGAGCAG CCGCAGCACC
    CAGAGGCTCG TGCTGCCCCC GCCTGCGCTC ACTGCCGCCG GCGTCTTCGC CAAGTTCCGG
    GACATCGCGC AGCTGGCAGG CAGCGCTTCC ACAACCAAGA AGATTGACGT CATCAAAGGC
    CTCTTTGTGG CCTGCCGTCA CTCAGAGGCA CGTTTCATTG CCAGAGCCCT GAGTGGACGG
    CTGCGCCTCG GGTTGGCTGA GCAGTCGGTG CTGGCGGCCC TCGCCCAGGC CGTGAGCCTC
    ACGCCCCCCA GTCAAGAATG CCCCCCCGCC GTGGTGGACG CCGGGAAGGG CAAGACGGCA
    GAAGCCAGAA AGACGTGGCT GGAGGAGCAA GGCATGATCC TGAAGCAGAC TTTCTGTGAG
    GTACCTGACC TGGAGCGGAT CGTCCCCGTG CTTCTGGAAC ACGGCCTGGA GCGGCTCCCG
    GAGCACTGCA GACTGAGCCC AGGGGTTCCC CTGAAACCAA TGTTGGCCCA CCCCACCCGG
    GGTGTCAGTG AGGTCCTGAA GCGCTTTGAG GAGGCAGCTT TCACCTGCGA GTACAAATAC
    GACGGGCAGA GGGCACAGAT CCACGTTCTG GAAGGCGGGG AGGTAAAGAT CTTCAGCAGG
    AATCAGGAGG ACAATACTGG AAGGTACCCC GACATTATCA GCCGCATCCC TAAGATTAAA
    CTCCCATCGG TCACATCCTT CATCCTAGAC ACAGAAGCTG TAGCTTGGGA CCGGGAAAAG
    AAGCAGATCC AGCCATTCCA AGTCCTCACC ACCCGCAAAC GCAAGGAGGT GGACGCGGCG
    GAGATCCAGG TGCAGGTGTG TCTGTATGCC TTCGATCTCA TTTACCTCAA CGGAAAGTCC
    CTGGTACGTG AGCCCCTCTC CCGACGCCGG CAGCTGCTCC GGGAGAACTT CGTGGAGACG
    GAGGGCGAGT TCGTCTTTGC CACCTCCCTA GACACCAAGG ACACGGAGCA GATCGCAGAG
    TTCTTGGAGC AGTCGGTGAA AGACTCCTGC GAGGGGCTGA TGGTGAAGAC CCTGGACGTG
    GACGCCACCT ACGAGATCGC CAAGAGGTCA CACAACTGGC TCAAGCTGAA GAAGGACTAC
    CTCGAGGGTG TGGGCGACAC CCTGGACCTC GTAGTGATTG GTGCCTACCT GGGCAAGGGG
    AAACGGGCCG GCCGGTACGG GGGCTTCCTG CTGGCCTCCT ATGACGAGGA GAGCGAGGAG
    TTCCAGGCCA TCTGCAAGCT CGGAACTGGC TTCAGTGACG AGGAGCTGGA AGAGCACCAC
    CAGAGGCTCC AGGCCTTAGT GCTGCCCACC CCGCGCTCCT ACGTTCGGGC TGACGGTGCG
    GTGGCCCCCG ACCACTGGCT GGACCCCAGC GACGTGTGGG AGGTGAAGTG CGCGGACCTC
    TCCCTGTCCC CCATCTACCC TGCGGCCCGA GGCCTGGTGG ACAGCGAGAA GGGGATCTCC
    CTCCGCTTCC CTCGGTTTAT TCGCGTCCGT GAGGATAAGA AGCCGGAGCA GGCCACTACC
    AGTGCCCAGG TGGCCGGTCT GTACAAGAAG CAGAGTCAGA TTCAGAACCA GCAGGGTGCG
    GAGCCGGACT CCGAGCAGGA AGAGTTCTAC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010813283.1
    CDS172..2964
    Translation

    Target ORF information:

    RefSeq Version XM_010814981.2
    Organism Bos taurus(cattle)
    Definition Bos taurus ligase I, DNA, ATP-dependent (LIG1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010814981.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGCAGCGAA GTATCATGTC ATTTTTCCAG CCCAAGAAAG AGGGCAAAGC GAAGAAGCCA 
    GAGAAGGAGA CAGCCAAGAG CATCAGAGAA ACAGAGTCCC CTCCGAAGGT GGCGCTGAAG
    GAGCGGAATC GAGTGGTGTC TGAGGGTGAC TCTCCAGTGA AGAGGCCAGG GAGGAAGGCG
    ACCCGGGTCC TGGGCAGCGA GGGCGAGGAG GAGGAAGAGG AAGCCCCCAC CACCCCCAGA
    AGCCAGAAGC CTGCTTCAGA CTCTCCACAG GCCTCGCCTC CCAGTCCTGT CACACTGCCT
    GAGAGCAGCC CTTCCCACTC CAAACCCTCC CCAGCAGTTG CATCCCCATC AGGGATCCCG
    AAGCGTCGCA CGGCCCGGAA GCAGCTTCCC AAACGGACAT TTCAAGATGT TCTGAAAGAG
    CAGGGCGAGG ACAAGGATCC AGAAACTAAG AAGAAGAAGG AGGAAGAAGC GGAGACACCA
    ATAGAAAGCC TCCCAGAGCC TGAAGGGGAC GACAAGGAGG AAGTGGAAGG TGGGGACCAG
    CCCACGACGC CCCCTGAGCC CCGGAAGACC TCCAAGTCCC TGACAGAAAG CGTCTCAGAG
    CCCGGGGTGA CGGTGAAGCA GGAACCACAG GAGGATGGCC AGGCTCAACC GCCCCCCAAA
    ACTCCAAAGA CCCTCAGCAG TTTCTTCTGT GAGTGGGGAC TTCACCTTGC GGGGCTGCGC
    AGGGAAGAGG CAGCCCCCCG GAAGCCAGCT GTCAAAAAAG AAGCGAAAGA AGAAGGACCT
    GGTGCTCTGA GAAAGGACGA GATCAAGGGA CACGTGGACC CAGCGAGCTA TAATCCTGCC
    AAGAACAACT ATCACCCCAT CGAAGATGCC TGCTGGAAGG CGGGCCAGAG GGTTCCTTAT
    CTGGCTGTGG CCCGGGCGTT CGAGAAGATC GAGGAGGTCT CTGCTCGGCT CCGGATGGTG
    GAGACGCTGA GCAACCTGCT GCGCTCCGTG GTGGCCCTGT CACCTGCTGA CCTCCTGCCC
    GTCCTCTACC TGAGCCTCAA CCGCCTGGGG CCGCCCCAGC AGGGCCTGGA GCTCGGCATC
    GGAGAGGGCA TCCTCCTCAA GGCAGTGGCC CAGGCCACGG GTCGGCAGCT GGAGTCCGTC
    CGGGCCGAGG TGGCCGAGAA GGGTGATGTG GGGCTGGTGG CCGAGAGCAG CCGCAGCACC
    CAGAGGCTCG TGCTGCCCCC GCCTGCGCTC ACTGCCGCCG GCGTCTTCGC CAAGTTCCGG
    GACATCGCGC AGCTGGCAGG CAGCGCTTCC ACAACCAAGA AGATTGACGT CATCAAAGGC
    CTCTTTGTGG CCTGCCGTCA CTCAGAGGCA CGTTTCATTG CCAGAGCCCT GAGTGGACGG
    CTGCGCCTCG GGTTGGCTGA GCAGTCGGTG CTGGCGGCCC TCGCCCAGGC CGTGAGCCTC
    ACGCCCCCCA GTCAAGAATG CCCCCCCGCC GTGGTGGACG CCGGGAAGGG CAAGACGGCA
    GAAGCCAGAA AGACGTGGCT GGAGGAGCAA GGCATGATCC TGAAGCAGAC TTTCTGTGAG
    GTACCTGACC TGGAGCGGAT CGTCCCCGTG CTTCTGGAAC ACGGCCTGGA GCGGCTCCCG
    GAGCACTGCA GACTGAGCCC AGGGGTTCCC CTGAAACCAA TGTTGGCCCA CCCCACCCGG
    GGTGTCAGTG AGGTCCTGAA GCGCTTTGAG GAGGCAGCTT TCACCTGCGA GTACAAATAC
    GACGGGCAGA GGGCACAGAT CCACGTTCTG GAAGGCGGGG AGGTAAAGAT CTTCAGCAGG
    AATCAGGAGG ACAATACTGG AAGGTACCCC GACATTATCA GCCGCATCCC TAAGATTAAA
    CTCCCATCGG TCACATCCTT CATCCTAGAC ACAGAAGCTG TAGCTTGGGA CCGGGAAAAG
    AAGCAGATCC AGCCATTCCA AGTCCTCACC ACCCGCAAAC GCAAGGAGGT GGACGCGGCG
    GAGATCCAGG TGCAGGTGTG TCTGTATGCC TTCGATCTCA TTTACCTCAA CGGAAAGTCC
    CTGGTACGTG AGCCCCTCTC CCGACGCCGG CAGCTGCTCC GGGAGAACTT CGTGGAGACG
    GAGGGCGAGT TCGTCTTTGC CACCTCCCTA GACACCAAGG ACACGGAGCA GATCGCAGAG
    TTCTTGGAGC AGTCGGTGAA AGACTCCTGC GAGGGGCTGA TGGTGAAGAC CCTGGACGTG
    GACGCCACCT ACGAGATCGC CAAGAGGTCA CACAACTGGC TCAAGCTGAA GAAGGACTAC
    CTCGAGGGTG TGGGCGACAC CCTGGACCTC GTAGTGATTG GTGCCTACCT GGGCAAGGGG
    AAACGGGCCG GCCGGTACGG GGGCTTCCTG CTGGCCTCCT ATGACGAGGA GAGCGAGGAG
    TTCCAGGCCA TCTGCAAGCT CGGAACTGGC TTCAGTGACG AGGAGCTGGA AGAGCACCAC
    CAGAGGCTCC AGGCCTTAGT GCTGCCCACC CCGCGCTCCT ACGTTCGGGC TGACGGTGCG
    GTGGCCCCCG ACCACTGGCT GGACCCCAGC GACGTGTGGG AGGTGAAGTG CGCGGACCTC
    TCCCTGTCCC CCATCTACCC TGCGGCCCGA GGCCTGGTGG ACAGCGAGAA GGGGATCTCC
    CTCCGCTTCC CTCGGTTTAT TCGCGTCCGT GAGGATAAGA AGCCGGAGCA GGCCACTACC
    AGTGCCCAGG TGGCCGGTCT GTACAAGAAG CAGAGTCAGA TTCAGAACCA GCAGGGTGCG
    GAGCCGGACT CCGAGCAGGA AGAGTTCTAC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa58685
    Clone ID Related Accession (Same CDS sequence) XM_010814982.2
    Accession Version XM_010814982.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2922bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA ligase 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104466.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010814982.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGAGAGGC TTGGACCGCA ACCCGAAGGA CTCCTGGTGA CCAGTCCCGC TCTACTCTGC 
    GGTTGCAGGA ACAGGAAACT GGTGACCAGC GGAGAGGAGG AGTGGGGAGA GAATTCTGAT
    ACCATCATGC AGCGAAGTAT CATGTCATTT TTCCAGCCCA AGAAAGAGGG CAAAGCGAAG
    AAGCCAGAGA AGGAGACAGC CAAGAGCATC AGAGAAACAG AGTCCCCTCC GAAGGTGGCG
    CTGAAGGAGC GGAATCGAGT GGTGTCTGAG GGTGACTCTC CAGTGAAGAG GCCAGGGAGG
    AAGGCGACCC GGGTCCTGGG CAGCGAGGGC GAGGAGGAGG AAGAGGAAGC CCCCACCACC
    CCCAGAAGCC AGAAGCCTGC TTCAGACTCT CCACAGGCCT CGCCTCCCAG TCCTGTCACA
    CTGCCTGAGA GCAGCCCTTC CCACTCCAAA CCCTCCCCAG CAGTTGCATC CCCATCAGGG
    ATCCCGAAGC GTCGCACGGC CCGGAAGCAG CTTCCCAAAC GGACATTTCA AGATGTTCTG
    AAAGAGCAGG GCGAGGACAA GGATCCAGAA ACTAAGAAGA AGAAGGAGGA AGAAGCGGAG
    ACACCAATAG AAAGCCTCCC AGAGCCTGAA GGGGACGACA AGGAGGAAGT GGAAGGTGGG
    GACCAGCCCA CGACGCCCCC TGAGCCCCGG AAGACCTCCA CAGAGTCCCT GACAGAAAGC
    GTCTCAGAGC CCGGGGTGAC GGTGAAGCAG GAACCACAGG AGGATGGCCA GGCTCAACCG
    CCCCCCAAAA CTCCAAAGAC CCTCAGCAGT TTCTTCTGTG AGTGGGGACT TCACCTTGCG
    GGGCTGCGCA GGGAAGAGGC AGCCCCCCGG AAGCCAGCTG TCAAAAAAGA AGCGAAAGAA
    GAAGGACCTG GTGCTCTGAG AAAGGACGAG ATCAAGGGAC ACGTGGACCC AGCGAGCTAT
    AATCCTGCCA AGAACAACTA TCACCCCATC GAAGATGCCT GCTGGAAGGC GGGCCAGAGG
    GTTCCTTATC TGGCTGTGGC CCGGGCGTTC GAGAAGATCG AGGAGGTCTC TGCTCGGCTC
    CGGATGGTGG AGACGCTGAG CAACCTGCTG CGCTCCGTGG TGGCCCTGTC ACCTGCTGAC
    CTCCTGCCCG TCCTCTACCT GAGCCTCAAC CGCCTGGGGC CGCCCCAGCA GGGCCTGGAG
    CTCGGCATCG GAGAGGGCAT CCTCCTCAAG GCAGTGGCCC AGGCCACGGG TCGGCAGCTG
    GAGTCCGTCC GGGCCGAGGT GGCCGAGAAG GGTGATGTGG GGCTGGTGGC CGAGAGCAGC
    CGCAGCACCC AGAGGCTCGT GCTGCCCCCG CCTGCGCTCA CTGCCGCCGG CGTCTTCGCC
    AAGTTCCGGG ACATCGCGCA GCTGGCAGGC AGCGCTTCCA CAACCAAGAA GATTGACGTC
    ATCAAAGGCC TCTTTGTGGC CTGCCGTCAC TCAGAGGCAC GTTTCATTGC CAGAGCCCTG
    AGTGGACGGC TGCGCCTCGG GTTGGCTGAG CAGTCGGTGC TGGCGGCCCT CGCCCAGGCC
    GTGAGCCTCA CGCCCCCCAG TCAAGAATGC CCCCCCGCCG TGGTGGACGC CGGGAAGGGC
    AAGACGGCAG AAGCCAGAAA GACGTGGCTG GAGGAGCAAG GCATGATCCT GAAGCAGACT
    TTCTGTGAGG TACCTGACCT GGAGCGGATC GTCCCCGTGC TTCTGGAACA CGGCCTGGAG
    CGGCTCCCGG AGCACTGCAG ACTGAGCCCA GGGGTTCCCC TGAAACCAAT GTTGGCCCAC
    CCCACCCGGG GTGTCAGTGA GGTCCTGAAG CGCTTTGAGG AGGCAGCTTT CACCTGCGAG
    TACAAATACG ACGGGCAGAG GGCACAGATC CACGTTCTGG AAGGCGGGGA GGTAAAGATC
    TTCAGCAGGA ATCAGGAGGA CAATACTGGA AGGTACCCCG ACATTATCAG CCGCATCCCT
    AAGATTAAAC TCCCATCGGT CACATCCTTC ATCCTAGACA CAGAAGCTGT AGCTTGGGAC
    CGGGAAAAGA AGCAGATCCA GCCATTCCAA GTCCTCACCA CCCGCAAACG CAAGGAGGTG
    GACGCGGCGG AGATCCAGGT GCAGGTGTGT CTGTATGCCT TCGATCTCAT TTACCTCAAC
    GGAAAGTCCC TGGTACGTGA GCCCCTCTCC CGACGCCGGC AGCTGCTCCG GGAGAACTTC
    GTGGAGACGG AGGGCGAGTT CGTCTTTGCC ACCTCCCTAG ACACCAAGGA CACGGAGCAG
    ATCGCAGAGT TCTTGGAGCA GTCGGTGAAA GACTCCTGCG AGGGGCTGAT GGTGAAGACC
    CTGGACGTGG ACGCCACCTA CGAGATCGCC AAGAGGTCAC ACAACTGGCT CAAGCTGAAG
    AAGGACTACC TCGAGGGTGT GGGCGACACC CTGGACCTCG TAGTGATTGG TGCCTACCTG
    GGCAAGGGGA AACGGGCCGG CCGGTACGGG GGCTTCCTGC TGGCCTCCTA TGACGAGGAG
    AGCGAGGAGT TCCAGGCCAT CTGCAAGCTC GGAACTGGCT TCAGTGACGA GGAGCTGGAA
    GAGCACCACC AGAGGCTCCA GGCCTTAGTG CTGCCCACCC CGCGCTCCTA CGTTCGGGCT
    GACGGTGCGG TGGCCCCCGA CCACTGGCTG GACCCCAGCG ACGTGTGGGA GGTGAAGTGC
    GCGGACCTCT CCCTGTCCCC CATCTACCCT GCGGCCCGAG GCCTGGTGGA CAGCGAGAAG
    GGGATCTCCC TCCGCTTCCC TCGGTTTATT CGCGTCCGTG AGGATAAGAA GCCGGAGCAG
    GCCACTACCA GTGCCCAGGT GGCCGGTCTG TACAAGAAGC AGAGTCAGAT TCAGAACCAG
    CAGGGTGCGG AGCCGGACTC CGAGCAGGAA GAGTTCTACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010813284.1
    CDS477..3398
    Translation

    Target ORF information:

    RefSeq Version XM_010814982.2
    Organism Bos taurus(cattle)
    Definition Bos taurus ligase I, DNA, ATP-dependent (LIG1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010814982.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGAGAGGC TTGGACCGCA ACCCGAAGGA CTCCTGGTGA CCAGTCCCGC TCTACTCTGC 
    GGTTGCAGGA ACAGGAAACT GGTGACCAGC GGAGAGGAGG AGTGGGGAGA GAATTCTGAT
    ACCATCATGC AGCGAAGTAT CATGTCATTT TTCCAGCCCA AGAAAGAGGG CAAAGCGAAG
    AAGCCAGAGA AGGAGACAGC CAAGAGCATC AGAGAAACAG AGTCCCCTCC GAAGGTGGCG
    CTGAAGGAGC GGAATCGAGT GGTGTCTGAG GGTGACTCTC CAGTGAAGAG GCCAGGGAGG
    AAGGCGACCC GGGTCCTGGG CAGCGAGGGC GAGGAGGAGG AAGAGGAAGC CCCCACCACC
    CCCAGAAGCC AGAAGCCTGC TTCAGACTCT CCACAGGCCT CGCCTCCCAG TCCTGTCACA
    CTGCCTGAGA GCAGCCCTTC CCACTCCAAA CCCTCCCCAG CAGTTGCATC CCCATCAGGG
    ATCCCGAAGC GTCGCACGGC CCGGAAGCAG CTTCCCAAAC GGACATTTCA AGATGTTCTG
    AAAGAGCAGG GCGAGGACAA GGATCCAGAA ACTAAGAAGA AGAAGGAGGA AGAAGCGGAG
    ACACCAATAG AAAGCCTCCC AGAGCCTGAA GGGGACGACA AGGAGGAAGT GGAAGGTGGG
    GACCAGCCCA CGACGCCCCC TGAGCCCCGG AAGACCTCCA CAGAGTCCCT GACAGAAAGC
    GTCTCAGAGC CCGGGGTGAC GGTGAAGCAG GAACCACAGG AGGATGGCCA GGCTCAACCG
    CCCCCCAAAA CTCCAAAGAC CCTCAGCAGT TTCTTCTGTG AGTGGGGACT TCACCTTGCG
    GGGCTGCGCA GGGAAGAGGC AGCCCCCCGG AAGCCAGCTG TCAAAAAAGA AGCGAAAGAA
    GAAGGACCTG GTGCTCTGAG AAAGGACGAG ATCAAGGGAC ACGTGGACCC AGCGAGCTAT
    AATCCTGCCA AGAACAACTA TCACCCCATC GAAGATGCCT GCTGGAAGGC GGGCCAGAGG
    GTTCCTTATC TGGCTGTGGC CCGGGCGTTC GAGAAGATCG AGGAGGTCTC TGCTCGGCTC
    CGGATGGTGG AGACGCTGAG CAACCTGCTG CGCTCCGTGG TGGCCCTGTC ACCTGCTGAC
    CTCCTGCCCG TCCTCTACCT GAGCCTCAAC CGCCTGGGGC CGCCCCAGCA GGGCCTGGAG
    CTCGGCATCG GAGAGGGCAT CCTCCTCAAG GCAGTGGCCC AGGCCACGGG TCGGCAGCTG
    GAGTCCGTCC GGGCCGAGGT GGCCGAGAAG GGTGATGTGG GGCTGGTGGC CGAGAGCAGC
    CGCAGCACCC AGAGGCTCGT GCTGCCCCCG CCTGCGCTCA CTGCCGCCGG CGTCTTCGCC
    AAGTTCCGGG ACATCGCGCA GCTGGCAGGC AGCGCTTCCA CAACCAAGAA GATTGACGTC
    ATCAAAGGCC TCTTTGTGGC CTGCCGTCAC TCAGAGGCAC GTTTCATTGC CAGAGCCCTG
    AGTGGACGGC TGCGCCTCGG GTTGGCTGAG CAGTCGGTGC TGGCGGCCCT CGCCCAGGCC
    GTGAGCCTCA CGCCCCCCAG TCAAGAATGC CCCCCCGCCG TGGTGGACGC CGGGAAGGGC
    AAGACGGCAG AAGCCAGAAA GACGTGGCTG GAGGAGCAAG GCATGATCCT GAAGCAGACT
    TTCTGTGAGG TACCTGACCT GGAGCGGATC GTCCCCGTGC TTCTGGAACA CGGCCTGGAG
    CGGCTCCCGG AGCACTGCAG ACTGAGCCCA GGGGTTCCCC TGAAACCAAT GTTGGCCCAC
    CCCACCCGGG GTGTCAGTGA GGTCCTGAAG CGCTTTGAGG AGGCAGCTTT CACCTGCGAG
    TACAAATACG ACGGGCAGAG GGCACAGATC CACGTTCTGG AAGGCGGGGA GGTAAAGATC
    TTCAGCAGGA ATCAGGAGGA CAATACTGGA AGGTACCCCG ACATTATCAG CCGCATCCCT
    AAGATTAAAC TCCCATCGGT CACATCCTTC ATCCTAGACA CAGAAGCTGT AGCTTGGGAC
    CGGGAAAAGA AGCAGATCCA GCCATTCCAA GTCCTCACCA CCCGCAAACG CAAGGAGGTG
    GACGCGGCGG AGATCCAGGT GCAGGTGTGT CTGTATGCCT TCGATCTCAT TTACCTCAAC
    GGAAAGTCCC TGGTACGTGA GCCCCTCTCC CGACGCCGGC AGCTGCTCCG GGAGAACTTC
    GTGGAGACGG AGGGCGAGTT CGTCTTTGCC ACCTCCCTAG ACACCAAGGA CACGGAGCAG
    ATCGCAGAGT TCTTGGAGCA GTCGGTGAAA GACTCCTGCG AGGGGCTGAT GGTGAAGACC
    CTGGACGTGG ACGCCACCTA CGAGATCGCC AAGAGGTCAC ACAACTGGCT CAAGCTGAAG
    AAGGACTACC TCGAGGGTGT GGGCGACACC CTGGACCTCG TAGTGATTGG TGCCTACCTG
    GGCAAGGGGA AACGGGCCGG CCGGTACGGG GGCTTCCTGC TGGCCTCCTA TGACGAGGAG
    AGCGAGGAGT TCCAGGCCAT CTGCAAGCTC GGAACTGGCT TCAGTGACGA GGAGCTGGAA
    GAGCACCACC AGAGGCTCCA GGCCTTAGTG CTGCCCACCC CGCGCTCCTA CGTTCGGGCT
    GACGGTGCGG TGGCCCCCGA CCACTGGCTG GACCCCAGCG ACGTGTGGGA GGTGAAGTGC
    GCGGACCTCT CCCTGTCCCC CATCTACCCT GCGGCCCGAG GCCTGGTGGA CAGCGAGAAG
    GGGATCTCCC TCCGCTTCCC TCGGTTTATT CGCGTCCGTG AGGATAAGAA GCCGGAGCAG
    GCCACTACCA GTGCCCAGGT GGCCGGTCTG TACAAGAAGC AGAGTCAGAT TCAGAACCAG
    CAGGGTGCGG AGCCGGACTC CGAGCAGGAA GAGTTCTACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa224759
    Clone ID Related Accession (Same CDS sequence) XM_010814980.3
    Accession Version XM_010814980.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2796bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DNA ligase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010814980.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGCAGCGAA GTATCATGTC ATTTTTCCAG CCCAAGAAAG AGGGCAAAGC GAAGAAGCCA 
    GAGAAGGAGA CAGCCAAGAG CATCAGAGAA ACAGAGTCCC CTCCGAAGGT GGCGCTGAAG
    GAGCGGAATC GAGTGGTGTC TGAGGGTGAC TCTCCAGTGA AGAGGCCAGG GAGGAAGGCG
    ACCCAGGTCC TGGGCAGCGA GGGCGAGGAG GAGGAAGAGG AAGCCCCCAC CACCCCCAGA
    AGCCAGAAGC CTGCTTCAGA CTCTCCACAG GCCTCGCCTC CCAGTCCTGT CACACTGCCT
    GAGAGCAGCC CTTCCCACTC CAAACCCTCC CCAGCAGTTG CATCCCCATC AGGGATCCCG
    AAGCGTCGCA CGGCCCGGAA GCAGCTTCCC AAACGGACAT TTCAAGATGT TCTGAAAGAG
    CAGGGCGAGG ACAAGGATCC AGAAACTAAG AAGAAGAAGG AGGAAGAAGC GGAGACACCA
    ATAGAAAGCC TCCCAGAGCC TGAAGGGGAC GACAAGGAGG AAGTGGAAGG TGGGGACCAG
    CCCACGACGC CCCCTGAGCC CCGGAAGACC TCCACAGAGT CCCTGACAGA AAGCGTCTCA
    GAGCCCGGGG TGACGGTGAA GCAGGAACCA CAGGAGGATG GCCAGGCTCA ACCGCCCCCC
    AAAACTCCAA AGACCCTCAG CAGTTTCTTC TGTGAGTGGG GACTTCACCT TGCGGGGCTG
    CGCAGGGAAG AGGCAGCCCC CCGGAAGCCA GCTGTCAAAA AAGAAGCGAA AGAAGAAGGA
    CCTGGTGCTC TGAGAAAGGA CGAGATCAAG GGACACGTGG ACCCAGCGAG CTATAATCCT
    GCCAAGAACA ACTATCACCC CATCGAAGAT GCCTGCTGGA AGGCGGGCCA GAGGGTTCCT
    TATCTGGCTG TGGCCCGGGC GTTCGAGAAG ATCGAGGAGG TCTCTGCTCG GCTCCGGATG
    GTGGAGACGC TGAGCAACCT GCTGCGCTCC GTGGTGGCCC TGTCACCTGC TGACCTCCTG
    CCCGTCCTCT ACCTGAGCCT CAACCGCCTG GGGCCGCCCC AGCAGGGCCT GGAGCTCGGC
    ATCGGAGAGG GCATCCTCCT CAAGGCAGTG GCCCAGGCCA CGGGTCGGCA GCTGGAGTCC
    GTCCGGGCCG AGGTGGCCGA GAAGGGTGAT GTGGGGCTGG TGGCCGAGAG CAGCCGCAGC
    ACCCAGAGGC TCGTGCTGCC CCCGCCTGCG CTCACTGCCG CCGGCGTCTT CGCCAAGTTC
    CGGGACATCG CGCAGCTGGC AGGCAGCGCT TCCACAACCA AGAAGATTGA CGTCATCAAA
    GGCCTCTTTG TGGCCTGCCG TCACTCAGAG GCACGTTTCA TTGCCAGAGC CCTGAGTGGA
    CGGCTGCGCC TCGGGTTGGC TGAGCAGTCG GTGCTGGCGG CCCTCGCCCA GGCCGTGAGC
    CTCACGCCCC CCAGTCAAGA ATGCCCCCCC GCCGTGGTGG ACGCCGGGAA GGGCAAGACG
    GCAGAAGCCA GAAAGACGTG GCTGGAGGAG CAAGGCATGA TCCTGAAGCA GACTTTCTGT
    GAGGTACCTG ACCTGGAGCG GATCGTCCCC GTGCTTCTGG AACACGGCCT GGAGCGGCTC
    CCGGAGCACT GCAGACTGAG CCCAGGGGTT CCCCTGAAAC CAATGTTGGC CCACCCCACC
    CGGGGTGTCA GTGAGGTCCT GAAGCGCTTT GAGGAGGCAG CTTTCACCTG CGAGTACAAA
    TACGACGGGC AGAGGGCACA GATCCACGTT CTGGAAGGCG GGGAGGTAAA GATCTTCAGC
    AGGAATCAGG AGGACAATAC TGGAAGGTAC CCCGACATTA TCAGCCGCAT CCCTAAGATT
    AAACTCCCAT CGGTCACATC CTTCATCCTA GACACAGAAG CTGTAGCTTG GGACCGGGAA
    AAGAAGCAGA TCCAGCCATT CCAAGTCCTC ACCACCCGCA AACGCAAGGA GGTGGACGCG
    GCGGAGATCC AGGTGCAGGT GTGTCTGTAT GCCTTCGATC TCATCTACCT CAACGGAGAG
    TCCCTGGTAC GTGAGCCCCT CTCCCGACGC CGGCAGCTGC TCCGGGAGAA CTTCGTGGAG
    ACGGAGGGCG AGTTCGTCTT TGCCACCTCC CTAGACACCA AGGACACGGA GCAGATCGCA
    GAGTTCTTGG AGCAGTCGGT GAAAGACTCC TGCGAGGGGC TGATGGTGAA GACCCTGGAT
    GTGGACGCCA CCTACGAGAT CGCCAAGAGG TCACACAACT GGCTCAAGCT GAAGAAGGAC
    TACCTCGAGG GTGTGGGCGA CACCCTGGAC CTCGTAGTGA TTGGTGCCTA CCTGGGCAAG
    GGGAAACGGG CCGGCCGGTA CGGGGGCTTC CTGCTGGCCT CCTATGACGA GGAGAGCGAG
    GAGTTCCAGG CCATCTGCAA GCTCGGAACT GGCTTCAGTG ACGAGGAGCT GGAAGAGCAC
    CACCAGAGGC TCCAGGCCTT AGTGCTGCCC ACCCCGCGCT CCTACGTTCG GGCTGACGGT
    GCGGTGGCCC CCGACCACTG GCTGGACCCC AGCGACGTGT GGGAGGTGAA GTGCGCGGAC
    CTCTCCCTGT CCCCCATCTA CCCTGCGGCC CGAGGCCTGG TGGACAGCGA GAAGGGGATC
    TCCCTCCGCT TCCCTCGGTT TATTCGCGTC CGTGAGGATA AGAAGCCGGA GCAGGCCACT
    ACCAGTGCCC AGGTGGCCGG TCTGTACAAG AAGCAGAGTC AGATTCAGAA CCAGCAGGGT
    GCGGAGCCGG ACTCCGAGCA GGAAGAGTTC TACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010813282.2
    CDS191..2986
    Translation

    Target ORF information:

    RefSeq Version XM_010814980.3
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA ligase 1 (LIG1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010814980.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGCAGCGAA GTATCATGTC ATTTTTCCAG CCCAAGAAAG AGGGCAAAGC GAAGAAGCCA 
    GAGAAGGAGA CAGCCAAGAG CATCAGAGAA ACAGAGTCCC CTCCGAAGGT GGCGCTGAAG
    GAGCGGAATC GAGTGGTGTC TGAGGGTGAC TCTCCAGTGA AGAGGCCAGG GAGGAAGGCG
    ACCCAGGTCC TGGGCAGCGA GGGCGAGGAG GAGGAAGAGG AAGCCCCCAC CACCCCCAGA
    AGCCAGAAGC CTGCTTCAGA CTCTCCACAG GCCTCGCCTC CCAGTCCTGT CACACTGCCT
    GAGAGCAGCC CTTCCCACTC CAAACCCTCC CCAGCAGTTG CATCCCCATC AGGGATCCCG
    AAGCGTCGCA CGGCCCGGAA GCAGCTTCCC AAACGGACAT TTCAAGATGT TCTGAAAGAG
    CAGGGCGAGG ACAAGGATCC AGAAACTAAG AAGAAGAAGG AGGAAGAAGC GGAGACACCA
    ATAGAAAGCC TCCCAGAGCC TGAAGGGGAC GACAAGGAGG AAGTGGAAGG TGGGGACCAG
    CCCACGACGC CCCCTGAGCC CCGGAAGACC TCCACAGAGT CCCTGACAGA AAGCGTCTCA
    GAGCCCGGGG TGACGGTGAA GCAGGAACCA CAGGAGGATG GCCAGGCTCA ACCGCCCCCC
    AAAACTCCAA AGACCCTCAG CAGTTTCTTC TGTGAGTGGG GACTTCACCT TGCGGGGCTG
    CGCAGGGAAG AGGCAGCCCC CCGGAAGCCA GCTGTCAAAA AAGAAGCGAA AGAAGAAGGA
    CCTGGTGCTC TGAGAAAGGA CGAGATCAAG GGACACGTGG ACCCAGCGAG CTATAATCCT
    GCCAAGAACA ACTATCACCC CATCGAAGAT GCCTGCTGGA AGGCGGGCCA GAGGGTTCCT
    TATCTGGCTG TGGCCCGGGC GTTCGAGAAG ATCGAGGAGG TCTCTGCTCG GCTCCGGATG
    GTGGAGACGC TGAGCAACCT GCTGCGCTCC GTGGTGGCCC TGTCACCTGC TGACCTCCTG
    CCCGTCCTCT ACCTGAGCCT CAACCGCCTG GGGCCGCCCC AGCAGGGCCT GGAGCTCGGC
    ATCGGAGAGG GCATCCTCCT CAAGGCAGTG GCCCAGGCCA CGGGTCGGCA GCTGGAGTCC
    GTCCGGGCCG AGGTGGCCGA GAAGGGTGAT GTGGGGCTGG TGGCCGAGAG CAGCCGCAGC
    ACCCAGAGGC TCGTGCTGCC CCCGCCTGCG CTCACTGCCG CCGGCGTCTT CGCCAAGTTC
    CGGGACATCG CGCAGCTGGC AGGCAGCGCT TCCACAACCA AGAAGATTGA CGTCATCAAA
    GGCCTCTTTG TGGCCTGCCG TCACTCAGAG GCACGTTTCA TTGCCAGAGC CCTGAGTGGA
    CGGCTGCGCC TCGGGTTGGC TGAGCAGTCG GTGCTGGCGG CCCTCGCCCA GGCCGTGAGC
    CTCACGCCCC CCAGTCAAGA ATGCCCCCCC GCCGTGGTGG ACGCCGGGAA GGGCAAGACG
    GCAGAAGCCA GAAAGACGTG GCTGGAGGAG CAAGGCATGA TCCTGAAGCA GACTTTCTGT
    GAGGTACCTG ACCTGGAGCG GATCGTCCCC GTGCTTCTGG AACACGGCCT GGAGCGGCTC
    CCGGAGCACT GCAGACTGAG CCCAGGGGTT CCCCTGAAAC CAATGTTGGC CCACCCCACC
    CGGGGTGTCA GTGAGGTCCT GAAGCGCTTT GAGGAGGCAG CTTTCACCTG CGAGTACAAA
    TACGACGGGC AGAGGGCACA GATCCACGTT CTGGAAGGCG GGGAGGTAAA GATCTTCAGC
    AGGAATCAGG AGGACAATAC TGGAAGGTAC CCCGACATTA TCAGCCGCAT CCCTAAGATT
    AAACTCCCAT CGGTCACATC CTTCATCCTA GACACAGAAG CTGTAGCTTG GGACCGGGAA
    AAGAAGCAGA TCCAGCCATT CCAAGTCCTC ACCACCCGCA AACGCAAGGA GGTGGACGCG
    GCGGAGATCC AGGTGCAGGT GTGTCTGTAT GCCTTCGATC TCATCTACCT CAACGGAGAG
    TCCCTGGTAC GTGAGCCCCT CTCCCGACGC CGGCAGCTGC TCCGGGAGAA CTTCGTGGAG
    ACGGAGGGCG AGTTCGTCTT TGCCACCTCC CTAGACACCA AGGACACGGA GCAGATCGCA
    GAGTTCTTGG AGCAGTCGGT GAAAGACTCC TGCGAGGGGC TGATGGTGAA GACCCTGGAT
    GTGGACGCCA CCTACGAGAT CGCCAAGAGG TCACACAACT GGCTCAAGCT GAAGAAGGAC
    TACCTCGAGG GTGTGGGCGA CACCCTGGAC CTCGTAGTGA TTGGTGCCTA CCTGGGCAAG
    GGGAAACGGG CCGGCCGGTA CGGGGGCTTC CTGCTGGCCT CCTATGACGA GGAGAGCGAG
    GAGTTCCAGG CCATCTGCAA GCTCGGAACT GGCTTCAGTG ACGAGGAGCT GGAAGAGCAC
    CACCAGAGGC TCCAGGCCTT AGTGCTGCCC ACCCCGCGCT CCTACGTTCG GGCTGACGGT
    GCGGTGGCCC CCGACCACTG GCTGGACCCC AGCGACGTGT GGGAGGTGAA GTGCGCGGAC
    CTCTCCCTGT CCCCCATCTA CCCTGCGGCC CGAGGCCTGG TGGACAGCGA GAAGGGGATC
    TCCCTCCGCT TCCCTCGGTT TATTCGCGTC CGTGAGGATA AGAAGCCGGA GCAGGCCACT
    ACCAGTGCCC AGGTGGCCGG TCTGTACAAG AAGCAGAGTC AGATTCAGAA CCAGCAGGGT
    GCGGAGCCGG ACTCCGAGCA GGAAGAGTTC TACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa224760
    Clone ID Related Accession (Same CDS sequence) XM_010814981.3
    Accession Version XM_010814981.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2793bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DNA ligase 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010814981.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGCAGCGAA GTATCATGTC ATTTTTCCAG CCCAAGAAAG AGGGCAAAGC GAAGAAGCCA 
    GAGAAGGAGA CAGCCAAGAG CATCAGAGAA ACAGAGTCCC CTCCGAAGGT GGCGCTGAAG
    GAGCGGAATC GAGTGGTGTC TGAGGGTGAC TCTCCAGTGA AGAGGCCAGG GAGGAAGGCG
    ACCCAGGTCC TGGGCAGCGA GGGCGAGGAG GAGGAAGAGG AAGCCCCCAC CACCCCCAGA
    AGCCAGAAGC CTGCTTCAGA CTCTCCACAG GCCTCGCCTC CCAGTCCTGT CACACTGCCT
    GAGAGCAGCC CTTCCCACTC CAAACCCTCC CCAGCAGTTG CATCCCCATC AGGGATCCCG
    AAGCGTCGCA CGGCCCGGAA GCAGCTTCCC AAACGGACAT TTCAAGATGT TCTGAAAGAG
    CAGGGCGAGG ACAAGGATCC AGAAACTAAG AAGAAGAAGG AGGAAGAAGC GGAGACACCA
    ATAGAAAGCC TCCCAGAGCC TGAAGGGGAC GACAAGGAGG AAGTGGAAGG TGGGGACCAG
    CCCACGACGC CCCCTGAGCC CCGGAAGACC TCCAAGTCCC TGACAGAAAG CGTCTCAGAG
    CCCGGGGTGA CGGTGAAGCA GGAACCACAG GAGGATGGCC AGGCTCAACC GCCCCCCAAA
    ACTCCAAAGA CCCTCAGCAG TTTCTTCTGT GAGTGGGGAC TTCACCTTGC GGGGCTGCGC
    AGGGAAGAGG CAGCCCCCCG GAAGCCAGCT GTCAAAAAAG AAGCGAAAGA AGAAGGACCT
    GGTGCTCTGA GAAAGGACGA GATCAAGGGA CACGTGGACC CAGCGAGCTA TAATCCTGCC
    AAGAACAACT ATCACCCCAT CGAAGATGCC TGCTGGAAGG CGGGCCAGAG GGTTCCTTAT
    CTGGCTGTGG CCCGGGCGTT CGAGAAGATC GAGGAGGTCT CTGCTCGGCT CCGGATGGTG
    GAGACGCTGA GCAACCTGCT GCGCTCCGTG GTGGCCCTGT CACCTGCTGA CCTCCTGCCC
    GTCCTCTACC TGAGCCTCAA CCGCCTGGGG CCGCCCCAGC AGGGCCTGGA GCTCGGCATC
    GGAGAGGGCA TCCTCCTCAA GGCAGTGGCC CAGGCCACGG GTCGGCAGCT GGAGTCCGTC
    CGGGCCGAGG TGGCCGAGAA GGGTGATGTG GGGCTGGTGG CCGAGAGCAG CCGCAGCACC
    CAGAGGCTCG TGCTGCCCCC GCCTGCGCTC ACTGCCGCCG GCGTCTTCGC CAAGTTCCGG
    GACATCGCGC AGCTGGCAGG CAGCGCTTCC ACAACCAAGA AGATTGACGT CATCAAAGGC
    CTCTTTGTGG CCTGCCGTCA CTCAGAGGCA CGTTTCATTG CCAGAGCCCT GAGTGGACGG
    CTGCGCCTCG GGTTGGCTGA GCAGTCGGTG CTGGCGGCCC TCGCCCAGGC CGTGAGCCTC
    ACGCCCCCCA GTCAAGAATG CCCCCCCGCC GTGGTGGACG CCGGGAAGGG CAAGACGGCA
    GAAGCCAGAA AGACGTGGCT GGAGGAGCAA GGCATGATCC TGAAGCAGAC TTTCTGTGAG
    GTACCTGACC TGGAGCGGAT CGTCCCCGTG CTTCTGGAAC ACGGCCTGGA GCGGCTCCCG
    GAGCACTGCA GACTGAGCCC AGGGGTTCCC CTGAAACCAA TGTTGGCCCA CCCCACCCGG
    GGTGTCAGTG AGGTCCTGAA GCGCTTTGAG GAGGCAGCTT TCACCTGCGA GTACAAATAC
    GACGGGCAGA GGGCACAGAT CCACGTTCTG GAAGGCGGGG AGGTAAAGAT CTTCAGCAGG
    AATCAGGAGG ACAATACTGG AAGGTACCCC GACATTATCA GCCGCATCCC TAAGATTAAA
    CTCCCATCGG TCACATCCTT CATCCTAGAC ACAGAAGCTG TAGCTTGGGA CCGGGAAAAG
    AAGCAGATCC AGCCATTCCA AGTCCTCACC ACCCGCAAAC GCAAGGAGGT GGACGCGGCG
    GAGATCCAGG TGCAGGTGTG TCTGTATGCC TTCGATCTCA TCTACCTCAA CGGAGAGTCC
    CTGGTACGTG AGCCCCTCTC CCGACGCCGG CAGCTGCTCC GGGAGAACTT CGTGGAGACG
    GAGGGCGAGT TCGTCTTTGC CACCTCCCTA GACACCAAGG ACACGGAGCA GATCGCAGAG
    TTCTTGGAGC AGTCGGTGAA AGACTCCTGC GAGGGGCTGA TGGTGAAGAC CCTGGATGTG
    GACGCCACCT ACGAGATCGC CAAGAGGTCA CACAACTGGC TCAAGCTGAA GAAGGACTAC
    CTCGAGGGTG TGGGCGACAC CCTGGACCTC GTAGTGATTG GTGCCTACCT GGGCAAGGGG
    AAACGGGCCG GCCGGTACGG GGGCTTCCTG CTGGCCTCCT ATGACGAGGA GAGCGAGGAG
    TTCCAGGCCA TCTGCAAGCT CGGAACTGGC TTCAGTGACG AGGAGCTGGA AGAGCACCAC
    CAGAGGCTCC AGGCCTTAGT GCTGCCCACC CCGCGCTCCT ACGTTCGGGC TGACGGTGCG
    GTGGCCCCCG ACCACTGGCT GGACCCCAGC GACGTGTGGG AGGTGAAGTG CGCGGACCTC
    TCCCTGTCCC CCATCTACCC TGCGGCCCGA GGCCTGGTGG ACAGCGAGAA GGGGATCTCC
    CTCCGCTTCC CTCGGTTTAT TCGCGTCCGT GAGGATAAGA AGCCGGAGCA GGCCACTACC
    AGTGCCCAGG TGGCCGGTCT GTACAAGAAG CAGAGTCAGA TTCAGAACCA GCAGGGTGCG
    GAGCCGGACT CCGAGCAGGA AGAGTTCTAC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010813283.2
    CDS191..2983
    Translation

    Target ORF information:

    RefSeq Version XM_010814981.3
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA ligase 1 (LIG1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010814981.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGCAGCGAA GTATCATGTC ATTTTTCCAG CCCAAGAAAG AGGGCAAAGC GAAGAAGCCA 
    GAGAAGGAGA CAGCCAAGAG CATCAGAGAA ACAGAGTCCC CTCCGAAGGT GGCGCTGAAG
    GAGCGGAATC GAGTGGTGTC TGAGGGTGAC TCTCCAGTGA AGAGGCCAGG GAGGAAGGCG
    ACCCAGGTCC TGGGCAGCGA GGGCGAGGAG GAGGAAGAGG AAGCCCCCAC CACCCCCAGA
    AGCCAGAAGC CTGCTTCAGA CTCTCCACAG GCCTCGCCTC CCAGTCCTGT CACACTGCCT
    GAGAGCAGCC CTTCCCACTC CAAACCCTCC CCAGCAGTTG CATCCCCATC AGGGATCCCG
    AAGCGTCGCA CGGCCCGGAA GCAGCTTCCC AAACGGACAT TTCAAGATGT TCTGAAAGAG
    CAGGGCGAGG ACAAGGATCC AGAAACTAAG AAGAAGAAGG AGGAAGAAGC GGAGACACCA
    ATAGAAAGCC TCCCAGAGCC TGAAGGGGAC GACAAGGAGG AAGTGGAAGG TGGGGACCAG
    CCCACGACGC CCCCTGAGCC CCGGAAGACC TCCAAGTCCC TGACAGAAAG CGTCTCAGAG
    CCCGGGGTGA CGGTGAAGCA GGAACCACAG GAGGATGGCC AGGCTCAACC GCCCCCCAAA
    ACTCCAAAGA CCCTCAGCAG TTTCTTCTGT GAGTGGGGAC TTCACCTTGC GGGGCTGCGC
    AGGGAAGAGG CAGCCCCCCG GAAGCCAGCT GTCAAAAAAG AAGCGAAAGA AGAAGGACCT
    GGTGCTCTGA GAAAGGACGA GATCAAGGGA CACGTGGACC CAGCGAGCTA TAATCCTGCC
    AAGAACAACT ATCACCCCAT CGAAGATGCC TGCTGGAAGG CGGGCCAGAG GGTTCCTTAT
    CTGGCTGTGG CCCGGGCGTT CGAGAAGATC GAGGAGGTCT CTGCTCGGCT CCGGATGGTG
    GAGACGCTGA GCAACCTGCT GCGCTCCGTG GTGGCCCTGT CACCTGCTGA CCTCCTGCCC
    GTCCTCTACC TGAGCCTCAA CCGCCTGGGG CCGCCCCAGC AGGGCCTGGA GCTCGGCATC
    GGAGAGGGCA TCCTCCTCAA GGCAGTGGCC CAGGCCACGG GTCGGCAGCT GGAGTCCGTC
    CGGGCCGAGG TGGCCGAGAA GGGTGATGTG GGGCTGGTGG CCGAGAGCAG CCGCAGCACC
    CAGAGGCTCG TGCTGCCCCC GCCTGCGCTC ACTGCCGCCG GCGTCTTCGC CAAGTTCCGG
    GACATCGCGC AGCTGGCAGG CAGCGCTTCC ACAACCAAGA AGATTGACGT CATCAAAGGC
    CTCTTTGTGG CCTGCCGTCA CTCAGAGGCA CGTTTCATTG CCAGAGCCCT GAGTGGACGG
    CTGCGCCTCG GGTTGGCTGA GCAGTCGGTG CTGGCGGCCC TCGCCCAGGC CGTGAGCCTC
    ACGCCCCCCA GTCAAGAATG CCCCCCCGCC GTGGTGGACG CCGGGAAGGG CAAGACGGCA
    GAAGCCAGAA AGACGTGGCT GGAGGAGCAA GGCATGATCC TGAAGCAGAC TTTCTGTGAG
    GTACCTGACC TGGAGCGGAT CGTCCCCGTG CTTCTGGAAC ACGGCCTGGA GCGGCTCCCG
    GAGCACTGCA GACTGAGCCC AGGGGTTCCC CTGAAACCAA TGTTGGCCCA CCCCACCCGG
    GGTGTCAGTG AGGTCCTGAA GCGCTTTGAG GAGGCAGCTT TCACCTGCGA GTACAAATAC
    GACGGGCAGA GGGCACAGAT CCACGTTCTG GAAGGCGGGG AGGTAAAGAT CTTCAGCAGG
    AATCAGGAGG ACAATACTGG AAGGTACCCC GACATTATCA GCCGCATCCC TAAGATTAAA
    CTCCCATCGG TCACATCCTT CATCCTAGAC ACAGAAGCTG TAGCTTGGGA CCGGGAAAAG
    AAGCAGATCC AGCCATTCCA AGTCCTCACC ACCCGCAAAC GCAAGGAGGT GGACGCGGCG
    GAGATCCAGG TGCAGGTGTG TCTGTATGCC TTCGATCTCA TCTACCTCAA CGGAGAGTCC
    CTGGTACGTG AGCCCCTCTC CCGACGCCGG CAGCTGCTCC GGGAGAACTT CGTGGAGACG
    GAGGGCGAGT TCGTCTTTGC CACCTCCCTA GACACCAAGG ACACGGAGCA GATCGCAGAG
    TTCTTGGAGC AGTCGGTGAA AGACTCCTGC GAGGGGCTGA TGGTGAAGAC CCTGGATGTG
    GACGCCACCT ACGAGATCGC CAAGAGGTCA CACAACTGGC TCAAGCTGAA GAAGGACTAC
    CTCGAGGGTG TGGGCGACAC CCTGGACCTC GTAGTGATTG GTGCCTACCT GGGCAAGGGG
    AAACGGGCCG GCCGGTACGG GGGCTTCCTG CTGGCCTCCT ATGACGAGGA GAGCGAGGAG
    TTCCAGGCCA TCTGCAAGCT CGGAACTGGC TTCAGTGACG AGGAGCTGGA AGAGCACCAC
    CAGAGGCTCC AGGCCTTAGT GCTGCCCACC CCGCGCTCCT ACGTTCGGGC TGACGGTGCG
    GTGGCCCCCG ACCACTGGCT GGACCCCAGC GACGTGTGGG AGGTGAAGTG CGCGGACCTC
    TCCCTGTCCC CCATCTACCC TGCGGCCCGA GGCCTGGTGG ACAGCGAGAA GGGGATCTCC
    CTCCGCTTCC CTCGGTTTAT TCGCGTCCGT GAGGATAAGA AGCCGGAGCA GGCCACTACC
    AGTGCCCAGG TGGCCGGTCT GTACAAGAAG CAGAGTCAGA TTCAGAACCA GCAGGGTGCG
    GAGCCGGACT CCGAGCAGGA AGAGTTCTAC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa224761
    Clone ID Related Accession (Same CDS sequence) XM_005219131.4
    Accession Version XM_005219131.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2748bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DNA ligase 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005219131.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCAGCGAA GTATCATGTC ATTTTTCCAG CCCAAGAAAG AGGGCAAAGC GAAGAAGCCA 
    GAGAAGGAGA CAGCCAAGAG CATCAGAGAA ACAGAGTCCC CTCCGAAGGT GGCGCTGAAG
    GAGCGGAATC GAGTGGTGTC TGAGGGTGAC TCTCCAGTGA AGAGGCCAGG GAGGAAGGCG
    ACCCAGGTCC TGGGCAGCGA GGGCGAGGAG GAGGAAGAGG AAGCCCCCAC CACCCCCAGA
    AGCCAGAAGC CTGCTTCAGA CTCTCCACAG GCCTCGCCTC CCAGTCCTGT CACACTGCCT
    GAGAGCAGCC CTTCCCACTC CAAACCCTCC CCAGCAGTTG CATCCCCATC AGGGATCCCG
    AAGCGTCGCA CGGCCCGGAA GCAGCTTCCC AAACGGACAT TTCAAGATGT TCTGAAAGAG
    CAGGGCGAGG ACAAGGATCC AGAAACTAAG AAGAAGAAGG AGGAAGAAGC GGAGACACCA
    ATAGAAAGCC TCCCAGAGCC TGAAGGGGAC GACAAGGAGG AAGTGGAAGG TGGGGACCAG
    CCCACGACGC CCCCTGAGCC CCGGAAGACC TCCAAGTCCC TGACAGAAAG CGTCTCAGAG
    CCCGGGGTGA CGGTGAAGCA GGAACCACAG GAGGATGGCC AGGCTCAACC GCCCCCCAAA
    ACTCCAAAGA CCCTCAGCAG TTTCTTCTCC CCCCGGAAGC CAGCTGTCAA AAAAGAAGCG
    AAAGAAGAAG GACCTGGTGC TCTGAGAAAG GACGAGATCA AGGGACACGT GGACCCAGCG
    AGCTATAATC CTGCCAAGAA CAACTATCAC CCCATCGAAG ATGCCTGCTG GAAGGCGGGC
    CAGAGGGTTC CTTATCTGGC TGTGGCCCGG GCGTTCGAGA AGATCGAGGA GGTCTCTGCT
    CGGCTCCGGA TGGTGGAGAC GCTGAGCAAC CTGCTGCGCT CCGTGGTGGC CCTGTCACCT
    GCTGACCTCC TGCCCGTCCT CTACCTGAGC CTCAACCGCC TGGGGCCGCC CCAGCAGGGC
    CTGGAGCTCG GCATCGGAGA GGGCATCCTC CTCAAGGCAG TGGCCCAGGC CACGGGTCGG
    CAGCTGGAGT CCGTCCGGGC CGAGGTGGCC GAGAAGGGTG ATGTGGGGCT GGTGGCCGAG
    AGCAGCCGCA GCACCCAGAG GCTCGTGCTG CCCCCGCCTG CGCTCACTGC CGCCGGCGTC
    TTCGCCAAGT TCCGGGACAT CGCGCAGCTG GCAGGCAGCG CTTCCACAAC CAAGAAGATT
    GACGTCATCA AAGGCCTCTT TGTGGCCTGC CGTCACTCAG AGGCACGTTT CATTGCCAGA
    GCCCTGAGTG GACGGCTGCG CCTCGGGTTG GCTGAGCAGT CGGTGCTGGC GGCCCTCGCC
    CAGGCCGTGA GCCTCACGCC CCCCAGTCAA GAATGCCCCC CCGCCGTGGT GGACGCCGGG
    AAGGGCAAGA CGGCAGAAGC CAGAAAGACG TGGCTGGAGG AGCAAGGCAT GATCCTGAAG
    CAGACTTTCT GTGAGGTACC TGACCTGGAG CGGATCGTCC CCGTGCTTCT GGAACACGGC
    CTGGAGCGGC TCCCGGAGCA CTGCAGACTG AGCCCAGGGG TTCCCCTGAA ACCAATGTTG
    GCCCACCCCA CCCGGGGTGT CAGTGAGGTC CTGAAGCGCT TTGAGGAGGC AGCTTTCACC
    TGCGAGTACA AATACGACGG GCAGAGGGCA CAGATCCACG TTCTGGAAGG CGGGGAGGTA
    AAGATCTTCA GCAGGAATCA GGAGGACAAT ACTGGAAGGT ACCCCGACAT TATCAGCCGC
    ATCCCTAAGA TTAAACTCCC ATCGGTCACA TCCTTCATCC TAGACACAGA AGCTGTAGCT
    TGGGACCGGG AAAAGAAGCA GATCCAGCCA TTCCAAGTCC TCACCACCCG CAAACGCAAG
    GAGGTGGACG CGGCGGAGAT CCAGGTGCAG GTGTGTCTGT ATGCCTTCGA TCTCATCTAC
    CTCAACGGAG AGTCCCTGGT ACGTGAGCCC CTCTCCCGAC GCCGGCAGCT GCTCCGGGAG
    AACTTCGTGG AGACGGAGGG CGAGTTCGTC TTTGCCACCT CCCTAGACAC CAAGGACACG
    GAGCAGATCG CAGAGTTCTT GGAGCAGTCG GTGAAAGACT CCTGCGAGGG GCTGATGGTG
    AAGACCCTGG ATGTGGACGC CACCTACGAG ATCGCCAAGA GGTCACACAA CTGGCTCAAG
    CTGAAGAAGG ACTACCTCGA GGGTGTGGGC GACACCCTGG ACCTCGTAGT GATTGGTGCC
    TACCTGGGCA AGGGGAAACG GGCCGGCCGG TACGGGGGCT TCCTGCTGGC CTCCTATGAC
    GAGGAGAGCG AGGAGTTCCA GGCCATCTGC AAGCTCGGAA CTGGCTTCAG TGACGAGGAG
    CTGGAAGAGC ACCACCAGAG GCTCCAGGCC TTAGTGCTGC CCACCCCGCG CTCCTACGTT
    CGGGCTGACG GTGCGGTGGC CCCCGACCAC TGGCTGGACC CCAGCGACGT GTGGGAGGTG
    AAGTGCGCGG ACCTCTCCCT GTCCCCCATC TACCCTGCGG CCCGAGGCCT GGTGGACAGC
    GAGAAGGGGA TCTCCCTCCG CTTCCCTCGG TTTATTCGCG TCCGTGAGGA TAAGAAGCCG
    GAGCAGGCCA CTACCAGTGC CCAGGTGGCC GGTCTGTACA AGAAGCAGAG TCAGATTCAG
    AACCAGCAGG GTGCGGAGCC GGACTCCGAG CAGGAAGAGT TCTACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005219188.2
    CDS191..2938
    Translation

    Target ORF information:

    RefSeq Version XM_005219131.4
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA ligase 1 (LIG1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005219131.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCAGCGAA GTATCATGTC ATTTTTCCAG CCCAAGAAAG AGGGCAAAGC GAAGAAGCCA 
    GAGAAGGAGA CAGCCAAGAG CATCAGAGAA ACAGAGTCCC CTCCGAAGGT GGCGCTGAAG
    GAGCGGAATC GAGTGGTGTC TGAGGGTGAC TCTCCAGTGA AGAGGCCAGG GAGGAAGGCG
    ACCCAGGTCC TGGGCAGCGA GGGCGAGGAG GAGGAAGAGG AAGCCCCCAC CACCCCCAGA
    AGCCAGAAGC CTGCTTCAGA CTCTCCACAG GCCTCGCCTC CCAGTCCTGT CACACTGCCT
    GAGAGCAGCC CTTCCCACTC CAAACCCTCC CCAGCAGTTG CATCCCCATC AGGGATCCCG
    AAGCGTCGCA CGGCCCGGAA GCAGCTTCCC AAACGGACAT TTCAAGATGT TCTGAAAGAG
    CAGGGCGAGG ACAAGGATCC AGAAACTAAG AAGAAGAAGG AGGAAGAAGC GGAGACACCA
    ATAGAAAGCC TCCCAGAGCC TGAAGGGGAC GACAAGGAGG AAGTGGAAGG TGGGGACCAG
    CCCACGACGC CCCCTGAGCC CCGGAAGACC TCCAAGTCCC TGACAGAAAG CGTCTCAGAG
    CCCGGGGTGA CGGTGAAGCA GGAACCACAG GAGGATGGCC AGGCTCAACC GCCCCCCAAA
    ACTCCAAAGA CCCTCAGCAG TTTCTTCTCC CCCCGGAAGC CAGCTGTCAA AAAAGAAGCG
    AAAGAAGAAG GACCTGGTGC TCTGAGAAAG GACGAGATCA AGGGACACGT GGACCCAGCG
    AGCTATAATC CTGCCAAGAA CAACTATCAC CCCATCGAAG ATGCCTGCTG GAAGGCGGGC
    CAGAGGGTTC CTTATCTGGC TGTGGCCCGG GCGTTCGAGA AGATCGAGGA GGTCTCTGCT
    CGGCTCCGGA TGGTGGAGAC GCTGAGCAAC CTGCTGCGCT CCGTGGTGGC CCTGTCACCT
    GCTGACCTCC TGCCCGTCCT CTACCTGAGC CTCAACCGCC TGGGGCCGCC CCAGCAGGGC
    CTGGAGCTCG GCATCGGAGA GGGCATCCTC CTCAAGGCAG TGGCCCAGGC CACGGGTCGG
    CAGCTGGAGT CCGTCCGGGC CGAGGTGGCC GAGAAGGGTG ATGTGGGGCT GGTGGCCGAG
    AGCAGCCGCA GCACCCAGAG GCTCGTGCTG CCCCCGCCTG CGCTCACTGC CGCCGGCGTC
    TTCGCCAAGT TCCGGGACAT CGCGCAGCTG GCAGGCAGCG CTTCCACAAC CAAGAAGATT
    GACGTCATCA AAGGCCTCTT TGTGGCCTGC CGTCACTCAG AGGCACGTTT CATTGCCAGA
    GCCCTGAGTG GACGGCTGCG CCTCGGGTTG GCTGAGCAGT CGGTGCTGGC GGCCCTCGCC
    CAGGCCGTGA GCCTCACGCC CCCCAGTCAA GAATGCCCCC CCGCCGTGGT GGACGCCGGG
    AAGGGCAAGA CGGCAGAAGC CAGAAAGACG TGGCTGGAGG AGCAAGGCAT GATCCTGAAG
    CAGACTTTCT GTGAGGTACC TGACCTGGAG CGGATCGTCC CCGTGCTTCT GGAACACGGC
    CTGGAGCGGC TCCCGGAGCA CTGCAGACTG AGCCCAGGGG TTCCCCTGAA ACCAATGTTG
    GCCCACCCCA CCCGGGGTGT CAGTGAGGTC CTGAAGCGCT TTGAGGAGGC AGCTTTCACC
    TGCGAGTACA AATACGACGG GCAGAGGGCA CAGATCCACG TTCTGGAAGG CGGGGAGGTA
    AAGATCTTCA GCAGGAATCA GGAGGACAAT ACTGGAAGGT ACCCCGACAT TATCAGCCGC
    ATCCCTAAGA TTAAACTCCC ATCGGTCACA TCCTTCATCC TAGACACAGA AGCTGTAGCT
    TGGGACCGGG AAAAGAAGCA GATCCAGCCA TTCCAAGTCC TCACCACCCG CAAACGCAAG
    GAGGTGGACG CGGCGGAGAT CCAGGTGCAG GTGTGTCTGT ATGCCTTCGA TCTCATCTAC
    CTCAACGGAG AGTCCCTGGT ACGTGAGCCC CTCTCCCGAC GCCGGCAGCT GCTCCGGGAG
    AACTTCGTGG AGACGGAGGG CGAGTTCGTC TTTGCCACCT CCCTAGACAC CAAGGACACG
    GAGCAGATCG CAGAGTTCTT GGAGCAGTCG GTGAAAGACT CCTGCGAGGG GCTGATGGTG
    AAGACCCTGG ATGTGGACGC CACCTACGAG ATCGCCAAGA GGTCACACAA CTGGCTCAAG
    CTGAAGAAGG ACTACCTCGA GGGTGTGGGC GACACCCTGG ACCTCGTAGT GATTGGTGCC
    TACCTGGGCA AGGGGAAACG GGCCGGCCGG TACGGGGGCT TCCTGCTGGC CTCCTATGAC
    GAGGAGAGCG AGGAGTTCCA GGCCATCTGC AAGCTCGGAA CTGGCTTCAG TGACGAGGAG
    CTGGAAGAGC ACCACCAGAG GCTCCAGGCC TTAGTGCTGC CCACCCCGCG CTCCTACGTT
    CGGGCTGACG GTGCGGTGGC CCCCGACCAC TGGCTGGACC CCAGCGACGT GTGGGAGGTG
    AAGTGCGCGG ACCTCTCCCT GTCCCCCATC TACCCTGCGG CCCGAGGCCT GGTGGACAGC
    GAGAAGGGGA TCTCCCTCCG CTTCCCTCGG TTTATTCGCG TCCGTGAGGA TAAGAAGCCG
    GAGCAGGCCA CTACCAGTGC CCAGGTGGCC GGTCTGTACA AGAAGCAGAG TCAGATTCAG
    AACCAGCAGG GTGCGGAGCC GGACTCCGAG CAGGAAGAGT TCTACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa224762
    Clone ID Related Accession (Same CDS sequence) XM_010814982.3
    Accession Version XM_010814982.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2922bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DNA ligase 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010814982.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGAGAGGC TTGGACCGCA ACCCGAAGGA CTCCTGGTGA CCAGTCCCGC TCTACTCTGC 
    GGTTGCAGGA ACAGGAAACT GGTGACCAGC GGAGAGGAGG AGTGGGGAGA GAATTCTGAT
    ACCATCATGC AGCGAAGTAT CATGTCATTT TTCCAGCCCA AGAAAGAGGG CAAAGCGAAG
    AAGCCAGAGA AGGAGACAGC CAAGAGCATC AGAGAAACAG AGTCCCCTCC GAAGGTGGCG
    CTGAAGGAGC GGAATCGAGT GGTGTCTGAG GGTGACTCTC CAGTGAAGAG GCCAGGGAGG
    AAGGCGACCC AGGTCCTGGG CAGCGAGGGC GAGGAGGAGG AAGAGGAAGC CCCCACCACC
    CCCAGAAGCC AGAAGCCTGC TTCAGACTCT CCACAGGCCT CGCCTCCCAG TCCTGTCACA
    CTGCCTGAGA GCAGCCCTTC CCACTCCAAA CCCTCCCCAG CAGTTGCATC CCCATCAGGG
    ATCCCGAAGC GTCGCACGGC CCGGAAGCAG CTTCCCAAAC GGACATTTCA AGATGTTCTG
    AAAGAGCAGG GCGAGGACAA GGATCCAGAA ACTAAGAAGA AGAAGGAGGA AGAAGCGGAG
    ACACCAATAG AAAGCCTCCC AGAGCCTGAA GGGGACGACA AGGAGGAAGT GGAAGGTGGG
    GACCAGCCCA CGACGCCCCC TGAGCCCCGG AAGACCTCCA CAGAGTCCCT GACAGAAAGC
    GTCTCAGAGC CCGGGGTGAC GGTGAAGCAG GAACCACAGG AGGATGGCCA GGCTCAACCG
    CCCCCCAAAA CTCCAAAGAC CCTCAGCAGT TTCTTCTGTG AGTGGGGACT TCACCTTGCG
    GGGCTGCGCA GGGAAGAGGC AGCCCCCCGG AAGCCAGCTG TCAAAAAAGA AGCGAAAGAA
    GAAGGACCTG GTGCTCTGAG AAAGGACGAG ATCAAGGGAC ACGTGGACCC AGCGAGCTAT
    AATCCTGCCA AGAACAACTA TCACCCCATC GAAGATGCCT GCTGGAAGGC GGGCCAGAGG
    GTTCCTTATC TGGCTGTGGC CCGGGCGTTC GAGAAGATCG AGGAGGTCTC TGCTCGGCTC
    CGGATGGTGG AGACGCTGAG CAACCTGCTG CGCTCCGTGG TGGCCCTGTC ACCTGCTGAC
    CTCCTGCCCG TCCTCTACCT GAGCCTCAAC CGCCTGGGGC CGCCCCAGCA GGGCCTGGAG
    CTCGGCATCG GAGAGGGCAT CCTCCTCAAG GCAGTGGCCC AGGCCACGGG TCGGCAGCTG
    GAGTCCGTCC GGGCCGAGGT GGCCGAGAAG GGTGATGTGG GGCTGGTGGC CGAGAGCAGC
    CGCAGCACCC AGAGGCTCGT GCTGCCCCCG CCTGCGCTCA CTGCCGCCGG CGTCTTCGCC
    AAGTTCCGGG ACATCGCGCA GCTGGCAGGC AGCGCTTCCA CAACCAAGAA GATTGACGTC
    ATCAAAGGCC TCTTTGTGGC CTGCCGTCAC TCAGAGGCAC GTTTCATTGC CAGAGCCCTG
    AGTGGACGGC TGCGCCTCGG GTTGGCTGAG CAGTCGGTGC TGGCGGCCCT CGCCCAGGCC
    GTGAGCCTCA CGCCCCCCAG TCAAGAATGC CCCCCCGCCG TGGTGGACGC CGGGAAGGGC
    AAGACGGCAG AAGCCAGAAA GACGTGGCTG GAGGAGCAAG GCATGATCCT GAAGCAGACT
    TTCTGTGAGG TACCTGACCT GGAGCGGATC GTCCCCGTGC TTCTGGAACA CGGCCTGGAG
    CGGCTCCCGG AGCACTGCAG ACTGAGCCCA GGGGTTCCCC TGAAACCAAT GTTGGCCCAC
    CCCACCCGGG GTGTCAGTGA GGTCCTGAAG CGCTTTGAGG AGGCAGCTTT CACCTGCGAG
    TACAAATACG ACGGGCAGAG GGCACAGATC CACGTTCTGG AAGGCGGGGA GGTAAAGATC
    TTCAGCAGGA ATCAGGAGGA CAATACTGGA AGGTACCCCG ACATTATCAG CCGCATCCCT
    AAGATTAAAC TCCCATCGGT CACATCCTTC ATCCTAGACA CAGAAGCTGT AGCTTGGGAC
    CGGGAAAAGA AGCAGATCCA GCCATTCCAA GTCCTCACCA CCCGCAAACG CAAGGAGGTG
    GACGCGGCGG AGATCCAGGT GCAGGTGTGT CTGTATGCCT TCGATCTCAT CTACCTCAAC
    GGAGAGTCCC TGGTACGTGA GCCCCTCTCC CGACGCCGGC AGCTGCTCCG GGAGAACTTC
    GTGGAGACGG AGGGCGAGTT CGTCTTTGCC ACCTCCCTAG ACACCAAGGA CACGGAGCAG
    ATCGCAGAGT TCTTGGAGCA GTCGGTGAAA GACTCCTGCG AGGGGCTGAT GGTGAAGACC
    CTGGATGTGG ACGCCACCTA CGAGATCGCC AAGAGGTCAC ACAACTGGCT CAAGCTGAAG
    AAGGACTACC TCGAGGGTGT GGGCGACACC CTGGACCTCG TAGTGATTGG TGCCTACCTG
    GGCAAGGGGA AACGGGCCGG CCGGTACGGG GGCTTCCTGC TGGCCTCCTA TGACGAGGAG
    AGCGAGGAGT TCCAGGCCAT CTGCAAGCTC GGAACTGGCT TCAGTGACGA GGAGCTGGAA
    GAGCACCACC AGAGGCTCCA GGCCTTAGTG CTGCCCACCC CGCGCTCCTA CGTTCGGGCT
    GACGGTGCGG TGGCCCCCGA CCACTGGCTG GACCCCAGCG ACGTGTGGGA GGTGAAGTGC
    GCGGACCTCT CCCTGTCCCC CATCTACCCT GCGGCCCGAG GCCTGGTGGA CAGCGAGAAG
    GGGATCTCCC TCCGCTTCCC TCGGTTTATT CGCGTCCGTG AGGATAAGAA GCCGGAGCAG
    GCCACTACCA GTGCCCAGGT GGCCGGTCTG TACAAGAAGC AGAGTCAGAT TCAGAACCAG
    CAGGGTGCGG AGCCGGACTC CGAGCAGGAA GAGTTCTACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010813284.2
    CDS520..3441
    Translation

    Target ORF information:

    RefSeq Version XM_010814982.3
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA ligase 1 (LIG1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010814982.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGAGAGGC TTGGACCGCA ACCCGAAGGA CTCCTGGTGA CCAGTCCCGC TCTACTCTGC 
    GGTTGCAGGA ACAGGAAACT GGTGACCAGC GGAGAGGAGG AGTGGGGAGA GAATTCTGAT
    ACCATCATGC AGCGAAGTAT CATGTCATTT TTCCAGCCCA AGAAAGAGGG CAAAGCGAAG
    AAGCCAGAGA AGGAGACAGC CAAGAGCATC AGAGAAACAG AGTCCCCTCC GAAGGTGGCG
    CTGAAGGAGC GGAATCGAGT GGTGTCTGAG GGTGACTCTC CAGTGAAGAG GCCAGGGAGG
    AAGGCGACCC AGGTCCTGGG CAGCGAGGGC GAGGAGGAGG AAGAGGAAGC CCCCACCACC
    CCCAGAAGCC AGAAGCCTGC TTCAGACTCT CCACAGGCCT CGCCTCCCAG TCCTGTCACA
    CTGCCTGAGA GCAGCCCTTC CCACTCCAAA CCCTCCCCAG CAGTTGCATC CCCATCAGGG
    ATCCCGAAGC GTCGCACGGC CCGGAAGCAG CTTCCCAAAC GGACATTTCA AGATGTTCTG
    AAAGAGCAGG GCGAGGACAA GGATCCAGAA ACTAAGAAGA AGAAGGAGGA AGAAGCGGAG
    ACACCAATAG AAAGCCTCCC AGAGCCTGAA GGGGACGACA AGGAGGAAGT GGAAGGTGGG
    GACCAGCCCA CGACGCCCCC TGAGCCCCGG AAGACCTCCA CAGAGTCCCT GACAGAAAGC
    GTCTCAGAGC CCGGGGTGAC GGTGAAGCAG GAACCACAGG AGGATGGCCA GGCTCAACCG
    CCCCCCAAAA CTCCAAAGAC CCTCAGCAGT TTCTTCTGTG AGTGGGGACT TCACCTTGCG
    GGGCTGCGCA GGGAAGAGGC AGCCCCCCGG AAGCCAGCTG TCAAAAAAGA AGCGAAAGAA
    GAAGGACCTG GTGCTCTGAG AAAGGACGAG ATCAAGGGAC ACGTGGACCC AGCGAGCTAT
    AATCCTGCCA AGAACAACTA TCACCCCATC GAAGATGCCT GCTGGAAGGC GGGCCAGAGG
    GTTCCTTATC TGGCTGTGGC CCGGGCGTTC GAGAAGATCG AGGAGGTCTC TGCTCGGCTC
    CGGATGGTGG AGACGCTGAG CAACCTGCTG CGCTCCGTGG TGGCCCTGTC ACCTGCTGAC
    CTCCTGCCCG TCCTCTACCT GAGCCTCAAC CGCCTGGGGC CGCCCCAGCA GGGCCTGGAG
    CTCGGCATCG GAGAGGGCAT CCTCCTCAAG GCAGTGGCCC AGGCCACGGG TCGGCAGCTG
    GAGTCCGTCC GGGCCGAGGT GGCCGAGAAG GGTGATGTGG GGCTGGTGGC CGAGAGCAGC
    CGCAGCACCC AGAGGCTCGT GCTGCCCCCG CCTGCGCTCA CTGCCGCCGG CGTCTTCGCC
    AAGTTCCGGG ACATCGCGCA GCTGGCAGGC AGCGCTTCCA CAACCAAGAA GATTGACGTC
    ATCAAAGGCC TCTTTGTGGC CTGCCGTCAC TCAGAGGCAC GTTTCATTGC CAGAGCCCTG
    AGTGGACGGC TGCGCCTCGG GTTGGCTGAG CAGTCGGTGC TGGCGGCCCT CGCCCAGGCC
    GTGAGCCTCA CGCCCCCCAG TCAAGAATGC CCCCCCGCCG TGGTGGACGC CGGGAAGGGC
    AAGACGGCAG AAGCCAGAAA GACGTGGCTG GAGGAGCAAG GCATGATCCT GAAGCAGACT
    TTCTGTGAGG TACCTGACCT GGAGCGGATC GTCCCCGTGC TTCTGGAACA CGGCCTGGAG
    CGGCTCCCGG AGCACTGCAG ACTGAGCCCA GGGGTTCCCC TGAAACCAAT GTTGGCCCAC
    CCCACCCGGG GTGTCAGTGA GGTCCTGAAG CGCTTTGAGG AGGCAGCTTT CACCTGCGAG
    TACAAATACG ACGGGCAGAG GGCACAGATC CACGTTCTGG AAGGCGGGGA GGTAAAGATC
    TTCAGCAGGA ATCAGGAGGA CAATACTGGA AGGTACCCCG ACATTATCAG CCGCATCCCT
    AAGATTAAAC TCCCATCGGT CACATCCTTC ATCCTAGACA CAGAAGCTGT AGCTTGGGAC
    CGGGAAAAGA AGCAGATCCA GCCATTCCAA GTCCTCACCA CCCGCAAACG CAAGGAGGTG
    GACGCGGCGG AGATCCAGGT GCAGGTGTGT CTGTATGCCT TCGATCTCAT CTACCTCAAC
    GGAGAGTCCC TGGTACGTGA GCCCCTCTCC CGACGCCGGC AGCTGCTCCG GGAGAACTTC
    GTGGAGACGG AGGGCGAGTT CGTCTTTGCC ACCTCCCTAG ACACCAAGGA CACGGAGCAG
    ATCGCAGAGT TCTTGGAGCA GTCGGTGAAA GACTCCTGCG AGGGGCTGAT GGTGAAGACC
    CTGGATGTGG ACGCCACCTA CGAGATCGCC AAGAGGTCAC ACAACTGGCT CAAGCTGAAG
    AAGGACTACC TCGAGGGTGT GGGCGACACC CTGGACCTCG TAGTGATTGG TGCCTACCTG
    GGCAAGGGGA AACGGGCCGG CCGGTACGGG GGCTTCCTGC TGGCCTCCTA TGACGAGGAG
    AGCGAGGAGT TCCAGGCCAT CTGCAAGCTC GGAACTGGCT TCAGTGACGA GGAGCTGGAA
    GAGCACCACC AGAGGCTCCA GGCCTTAGTG CTGCCCACCC CGCGCTCCTA CGTTCGGGCT
    GACGGTGCGG TGGCCCCCGA CCACTGGCTG GACCCCAGCG ACGTGTGGGA GGTGAAGTGC
    GCGGACCTCT CCCTGTCCCC CATCTACCCT GCGGCCCGAG GCCTGGTGGA CAGCGAGAAG
    GGGATCTCCC TCCGCTTCCC TCGGTTTATT CGCGTCCGTG AGGATAAGAA GCCGGAGCAG
    GCCACTACCA GTGCCCAGGT GGCCGGTCTG TACAAGAAGC AGAGTCAGAT TCAGAACCAG
    CAGGGTGCGG AGCCGGACTC CGAGCAGGAA GAGTTCTACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.