LIG1 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_010814980.2	XP_010813282.1	DNA ligase 1 isoform X1
XM_010814980.3	XP_010813282.2	DNA ligase 1 isoform X1
XM_010814981.3	XP_010813283.2	DNA ligase 1 isoform X2
XM_010814982.3	XP_010813284.2	DNA ligase 1 isoform X4
XM_005219131.4	XP_005219188.2	DNA ligase 1 isoform X3
XM_005219131.3	XP_005219188.1	DNA ligase 1 isoform X3
NM_001102548.1	NP_001096018.1	DNA ligase 1
XM_010814981.2	XP_010813283.1	DNA ligase 1 isoform X2
XM_010814982.2	XP_010813284.1	DNA ligase 1 isoform X4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa40221	NM_001102548.1 Latest version!	Bos taurus DNA ligase 1 (LIG1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa28788	XM_005219131.3 Latest version!	Bos taurus ligase I, DNA, ATP-dependent (LIG1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa58683	XM_010814980.2 Latest version!	Bos taurus ligase I, DNA, ATP-dependent (LIG1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa58684	XM_010814981.2 Latest version!	Bos taurus ligase I, DNA, ATP-dependent (LIG1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa58685	XM_010814982.2 Latest version!	Bos taurus ligase I, DNA, ATP-dependent (LIG1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa224759	XM_010814980.3 Latest version!	Bos taurus DNA ligase 1 (LIG1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa224760	XM_010814981.3 Latest version!	Bos taurus DNA ligase 1 (LIG1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa224761	XM_005219131.4 Latest version!	Bos taurus DNA ligase 1 (LIG1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa224762	XM_010814982.3 Latest version!	Bos taurus DNA ligase 1 (LIG1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

LIG1 ( NM_001102548.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001096018.1 Bos taurus DNA ligase 1 (LIG1), mRNA.

CloneID	OBa40221
Clone ID Related Accession (Same CDS sequence)	NM_001102548.1
Accession Version	NM_001102548.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2751bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-13
Organism	Bos taurus(cattle)
Product	DNA ligase 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC134518.1. ##Evidence-Data-START## Transcript exon combination :: BC134518.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGCAGCGAA GTATCATGTC ATTTTTCCAG CCCAAGAAAG AGGGCAAAGC GAAGAAGCCA 
GAGAAGGAGA CAGCCAAGAG CATCAGAGAA ACAGAGTCCC CTCCGAAGGT GGCGCTGAAG 
GAGCGGAATC GAGTGGTGTC TGAGGGTGAC TCTCCAGTGA AGAGGCCAGG GAGGAAGGCG 
ACCCGGGTCC TGGGCAGCGA GGGCGAGGAG GAGGAAGAGG AAGCCCCCAC CACCCCCAGA 
AGCCAGAAGC CTGCTTCAGA CTCTCCACAG GCCTCGCCTC CCAGTCCTGT CACACTGCCT 
GAGAGCAGCC CTTCCCACTC CAAACCCTCC CCAGCAGTTG CATCCCCATC AGGGATCCCG 
AAGCGTCGCA CGGCCCGGAA GCAGCTTCCC AAACGGACAT TTCAAGATGT TCTGAAAGAG 
CAGGGCGAGG ACAAGGATCC AGAAACTAAG AAGAAGAAGG AGGAAGAAGC GGAGACACCA 
ATAGAAAGCC TCCCAGAGCC TGAAGGGGAC GACAAGGAGG AAGTGGAAGG TGGGGACCAG 
CCCACGACGC CCCCTGAGCC CCGGAAGACC TCCACAGAGT CCCTGACAGA AAGCGTCTCA 
GAGCCCGGGG TGACGGTGAA GCAGGAACCA CAGGAGGATG GCCAGGCTCA ACCGCCCCCC 
AAAACTCCAA AGACCCTCAG CAGTTTCTTC TCCCCCCGGA AGCCAGCTGT CAAAAAAGAA 
GCGAAAGAAG AAGGACCTGG TGCTCTGAGA AAGGACGAGA TCAAGGGACA CGTGGACCCA 
GCGAGCTATA ATCCTGCCAA GAACAACTAT CACCCCATCG AAGATGCCTG CTGGAAGGCG 
GGCCAGAGGG TTCCTTATCT GGCTGTGGCC CGGGCGTTCG AGAAGATCGA GGAGGTCTCT 
GCTCGGCTCC GGATGGTGGA GACGCTGAGC AACCTGCTGC GCTCCGTGGT GGCCCTGTCA 
CCTGCTGACC TCCTGCCCGT CCTCTACCTG AGCCTCAACC GCCTGGGGCC GCCCCAGCAG 
GGCCTGGAGC TCGGCATCGG AGAGGGCATC CTCCTCAAGG CAGTGGCCCA GGCCACGGGT 
CGGCAGCTGG AGTCCGTCCG GGCCGAGGTG GCCGAGAAGG GTGATGTGGG GCTGGTGGCC 
GAGAGCAGCC GCAGCACCCA GAGGCTCGTG CTGCCCCCGC CTGCGCTCAC TGCCGCCGGC 
GTCTTCGCCA AGTTCCGGGA CATCGCGCAG CTGGCAGGCA GCGCTTCCAC AACCAAGAAG 
ATTGACGTCA TCAAAGGCCT CTTTGTGGCC TGCCGTCACT CAGAGGCACG TTTCATTGCC 
AGAGCCCTGA GTGGACGGCT GCGCCTCGGG TTGGCTGAGC AGTCGGTGCT GGCGGCCCTC 
GCCCAGGCCG TGAGCCTCAC GCCCCCCAGT CAAGAATGCC CCCCCGCCGT GGTGGACGCC 
GGGAAGGGCA AGACGGCAGA AGCCAGAAAG ACGTGGCTGG AGGAGCAAGG CATGATCCTG 
AAGCAGACTT TCTGTGAGGT ACCTGACCTG GAGCGGATCG TCCCCGTGCT TCTGGAACAC 
GGCCTGGAGC GGCTCCCGGA GCACTGCAGA CTGAGCCCAG GGGTTCCCCT GAAACCAATG 
TTGGCCCACC CCACCCGGGG TGTCAGTGAG GTCCTGAAGC GCTTTGAGGA GGCAGCTTTC 
ACCTGCGAGT ACAAATACGA CGGGCAGAGG GCACAGATCC ACGTTCTGGA AGGCGGGGAG 
GTAAAGATCT TCAGCAGGAA TCAGGAGGAC AATACTGGAA GGTACCCCGA CATTATCAGC 
CGCATCCCTA AGATTAAACT CCCATCGGTC ACATCCTTCA TCCTAGACAC AGAAGCTGTA 
GCTTGGGACC GGGAAAAGAA GCAGATCCAG CCATTCCAAG TCCTCACCAC CCGCAAACGC 
AAGGAGGTGG ACGCGGCGGA GATCCAGGTG CAGGTGTGTC TGTATGCCTT CGATCTCATT 
TACCTCAACG GAAAGTCCCT GGTACGTGAG CCCCTCTCCC GACGCCGGCA GCTGCTCCGG 
GAGAACTTCG TGGAGACGGA GGGCGAGTTC GTCTTTGCCA CCTCCCTAGA CACCAAGGAC 
ACGGAGCAGA TCGCAGAGTT CTTGGAGCAG TCGGTGAAAG ACTCCTGCGA GGGGCTGATG 
GTGAAGACCC TGGACGTGGA CGCCACCTAC GAGATCGCCA AGAGGTCACA CAACTGGCTC 
AAGCTGAAGA AGGACTACCT CGAGGGTGTG GGCGACACCC TGGACCTCGT AGTGATTGGT 
GCCTACCTGG GCAAGGGGAA ACGGGCCGGC CGGTACGGGG GCTTCCTGCT GGCCTCCTAT 
GACGAGGAGA GCGAGGAGTT CCAGGCCATC TGCAAGCTCG GAACTGGCTT CAGTGACGAG 
GAGCTGGAAG AGCACCACCA GAGGCTCCAG GCCTTAGTGC TGCCCACCCC GCGCTCCTAC 
GTTCGGGCTG ACGGTGCGGT GGCCCCCGAC CACTGGCTGG ACCCCAGCGA CGTGTGGGAG 
GTGAAGTGCG CGGACCTCTC CCTGTCCCCC ATCTACCCTG CGGCCCGAGG CCTGGTGGAC 
AGCGAGAAGG GGATCTCCCT CCGCTTCCCT CGGTTTATTC GCGTCCGTGA GGATAAGAAG 
CCGGAGCAGG CCACTACCAG TGCCCAGGTG GCCGGTCTGT ACAAGAAGCA GAGTCAGATT 
CAGAACCAGC AGGGTGCGGA GCCGGACTCC GAGCAGGAAG AGTTCTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001096018.1
CDS	122..2872
Translation	MQRSIMSFFQPKKEGKAKKPEKETAKSIRETESPPKVALKERNRVVSEGDSPVKRPGRKATRVLGSEGEEEEEEAPTTPRSQKPASDSPQASPPSPVTLPESSPSHSKPSPAVASPSGIPKRRTARKQLPKRTFQDVLKEQGEDKDPETKKKKEEEAETPIESLPEPEGDDKEEVEGGDQPTTPPEPRKTSTESLTESVSEPGVTVKQEPQEDGQAQPPPKTPKTLSSFFSPRKPAVKKEAKEEGPGALRKDEIKGHVDPASYNPAKNNYHPIEDACWKAGQRVPYLAVARAFEKIEEVSARLRMVETLSNLLRSVVALSPADLLPVLYLSLNRLGPPQQGLELGIGEGILLKAVAQATGRQLESVRAEVAEKGDVGLVAESSRSTQRLVLPPPALTAAGVFAKFRDIAQLAGSASTTKKIDVIKGLFVACRHSEARFIARALSGRLRLGLAEQSVLAALAQAVSLTPPSQECPPAVVDAGKGKTAEARKTWLEEQGMILKQTFCEVPDLERIVPVLLEHGLERLPEHCRLSPGVPLKPMLAHPTRGVSEVLKRFEEAAFTCEYKYDGQRAQIHVLEGGEVKIFSRNQEDNTGRYPDIISRIPKIKLPSVTSFILDTEAVAWDREKKQIQPFQVLTTRKRKEVDAAEIQVQVCLYAFDLIYLNGKSLVREPLSRRRQLLRENFVETEGEFVFATSLDTKDTEQIAEFLEQSVKDSCEGLMVKTLDVDATYEIAKRSHNWLKLKKDYLEGVGDTLDLVVIGAYLGKGKRAGRYGGFLLASYDEESEEFQAICKLGTGFSDEELEEHHQRLQALVLPTPRSYVRADGAVAPDHWLDPSDVWEVKCADLSLSPIYPAARGLVDSEKGISLRFPRFIRVREDKKPEQATTSAQVAGLYKKQSQIQNQQGAEPDSEQEEFY

Target ORF information:

RefSeq Version	NM_001102548.1
Organism	Bos taurus(cattle)
Definition	Bos taurus DNA ligase 1 (LIG1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001102548.1

ORF Insert Sequence:

ATGCAGCGAA GTATCATGTC ATTTTTCCAG CCCAAGAAAG AGGGCAAAGC GAAGAAGCCA 
GAGAAGGAGA CAGCCAAGAG CATCAGAGAA ACAGAGTCCC CTCCGAAGGT GGCGCTGAAG 
GAGCGGAATC GAGTGGTGTC TGAGGGTGAC TCTCCAGTGA AGAGGCCAGG GAGGAAGGCG 
ACCCGGGTCC TGGGCAGCGA GGGCGAGGAG GAGGAAGAGG AAGCCCCCAC CACCCCCAGA 
AGCCAGAAGC CTGCTTCAGA CTCTCCACAG GCCTCGCCTC CCAGTCCTGT CACACTGCCT 
GAGAGCAGCC CTTCCCACTC CAAACCCTCC CCAGCAGTTG CATCCCCATC AGGGATCCCG 
AAGCGTCGCA CGGCCCGGAA GCAGCTTCCC AAACGGACAT TTCAAGATGT TCTGAAAGAG 
CAGGGCGAGG ACAAGGATCC AGAAACTAAG AAGAAGAAGG AGGAAGAAGC GGAGACACCA 
ATAGAAAGCC TCCCAGAGCC TGAAGGGGAC GACAAGGAGG AAGTGGAAGG TGGGGACCAG 
CCCACGACGC CCCCTGAGCC CCGGAAGACC TCCACAGAGT CCCTGACAGA AAGCGTCTCA 
GAGCCCGGGG TGACGGTGAA GCAGGAACCA CAGGAGGATG GCCAGGCTCA ACCGCCCCCC 
AAAACTCCAA AGACCCTCAG CAGTTTCTTC TCCCCCCGGA AGCCAGCTGT CAAAAAAGAA 
GCGAAAGAAG AAGGACCTGG TGCTCTGAGA AAGGACGAGA TCAAGGGACA CGTGGACCCA 
GCGAGCTATA ATCCTGCCAA GAACAACTAT CACCCCATCG AAGATGCCTG CTGGAAGGCG 
GGCCAGAGGG TTCCTTATCT GGCTGTGGCC CGGGCGTTCG AGAAGATCGA GGAGGTCTCT 
GCTCGGCTCC GGATGGTGGA GACGCTGAGC AACCTGCTGC GCTCCGTGGT GGCCCTGTCA 
CCTGCTGACC TCCTGCCCGT CCTCTACCTG AGCCTCAACC GCCTGGGGCC GCCCCAGCAG 
GGCCTGGAGC TCGGCATCGG AGAGGGCATC CTCCTCAAGG CAGTGGCCCA GGCCACGGGT 
CGGCAGCTGG AGTCCGTCCG GGCCGAGGTG GCCGAGAAGG GTGATGTGGG GCTGGTGGCC 
GAGAGCAGCC GCAGCACCCA GAGGCTCGTG CTGCCCCCGC CTGCGCTCAC TGCCGCCGGC 
GTCTTCGCCA AGTTCCGGGA CATCGCGCAG CTGGCAGGCA GCGCTTCCAC AACCAAGAAG 
ATTGACGTCA TCAAAGGCCT CTTTGTGGCC TGCCGTCACT CAGAGGCACG TTTCATTGCC 
AGAGCCCTGA GTGGACGGCT GCGCCTCGGG TTGGCTGAGC AGTCGGTGCT GGCGGCCCTC 
GCCCAGGCCG TGAGCCTCAC GCCCCCCAGT CAAGAATGCC CCCCCGCCGT GGTGGACGCC 
GGGAAGGGCA AGACGGCAGA AGCCAGAAAG ACGTGGCTGG AGGAGCAAGG CATGATCCTG 
AAGCAGACTT TCTGTGAGGT ACCTGACCTG GAGCGGATCG TCCCCGTGCT TCTGGAACAC 
GGCCTGGAGC GGCTCCCGGA GCACTGCAGA CTGAGCCCAG GGGTTCCCCT GAAACCAATG 
TTGGCCCACC CCACCCGGGG TGTCAGTGAG GTCCTGAAGC GCTTTGAGGA GGCAGCTTTC 
ACCTGCGAGT ACAAATACGA CGGGCAGAGG GCACAGATCC ACGTTCTGGA AGGCGGGGAG 
GTAAAGATCT TCAGCAGGAA TCAGGAGGAC AATACTGGAA GGTACCCCGA CATTATCAGC 
CGCATCCCTA AGATTAAACT CCCATCGGTC ACATCCTTCA TCCTAGACAC AGAAGCTGTA 
GCTTGGGACC GGGAAAAGAA GCAGATCCAG CCATTCCAAG TCCTCACCAC CCGCAAACGC 
AAGGAGGTGG ACGCGGCGGA GATCCAGGTG CAGGTGTGTC TGTATGCCTT CGATCTCATT 
TACCTCAACG GAAAGTCCCT GGTACGTGAG CCCCTCTCCC GACGCCGGCA GCTGCTCCGG 
GAGAACTTCG TGGAGACGGA GGGCGAGTTC GTCTTTGCCA CCTCCCTAGA CACCAAGGAC 
ACGGAGCAGA TCGCAGAGTT CTTGGAGCAG TCGGTGAAAG ACTCCTGCGA GGGGCTGATG 
GTGAAGACCC TGGACGTGGA CGCCACCTAC GAGATCGCCA AGAGGTCACA CAACTGGCTC 
AAGCTGAAGA AGGACTACCT CGAGGGTGTG GGCGACACCC TGGACCTCGT AGTGATTGGT 
GCCTACCTGG GCAAGGGGAA ACGGGCCGGC CGGTACGGGG GCTTCCTGCT GGCCTCCTAT 
GACGAGGAGA GCGAGGAGTT CCAGGCCATC TGCAAGCTCG GAACTGGCTT CAGTGACGAG 
GAGCTGGAAG AGCACCACCA GAGGCTCCAG GCCTTAGTGC TGCCCACCCC GCGCTCCTAC 
GTTCGGGCTG ACGGTGCGGT GGCCCCCGAC CACTGGCTGG ACCCCAGCGA CGTGTGGGAG 
GTGAAGTGCG CGGACCTCTC CCTGTCCCCC ATCTACCCTG CGGCCCGAGG CCTGGTGGAC 
AGCGAGAAGG GGATCTCCCT CCGCTTCCCT CGGTTTATTC GCGTCCGTGA GGATAAGAAG 
CCGGAGCAGG CCACTACCAG TGCCCAGGTG GCCGGTCTGT ACAAGAAGCA GAGTCAGATT 
CAGAACCAGC AGGGTGCGGA GCCGGACTCC GAGCAGGAAG AGTTCTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIG1 ( XM_005219131.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005219188.1 Bos taurus ligase I, DNA, ATP-dependent (LIG1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa28788
Clone ID Related Accession (Same CDS sequence)	XM_005219131.3
Accession Version	XM_005219131.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2748bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	DNA ligase 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104466.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005219131.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCGAA GTATCATGTC ATTTTTCCAG CCCAAGAAAG AGGGCAAAGC GAAGAAGCCA 
GAGAAGGAGA CAGCCAAGAG CATCAGAGAA ACAGAGTCCC CTCCGAAGGT GGCGCTGAAG 
GAGCGGAATC GAGTGGTGTC TGAGGGTGAC TCTCCAGTGA AGAGGCCAGG GAGGAAGGCG 
ACCCGGGTCC TGGGCAGCGA GGGCGAGGAG GAGGAAGAGG AAGCCCCCAC CACCCCCAGA 
AGCCAGAAGC CTGCTTCAGA CTCTCCACAG GCCTCGCCTC CCAGTCCTGT CACACTGCCT 
GAGAGCAGCC CTTCCCACTC CAAACCCTCC CCAGCAGTTG CATCCCCATC AGGGATCCCG 
AAGCGTCGCA CGGCCCGGAA GCAGCTTCCC AAACGGACAT TTCAAGATGT TCTGAAAGAG 
CAGGGCGAGG ACAAGGATCC AGAAACTAAG AAGAAGAAGG AGGAAGAAGC GGAGACACCA 
ATAGAAAGCC TCCCAGAGCC TGAAGGGGAC GACAAGGAGG AAGTGGAAGG TGGGGACCAG 
CCCACGACGC CCCCTGAGCC CCGGAAGACC TCCAAGTCCC TGACAGAAAG CGTCTCAGAG 
CCCGGGGTGA CGGTGAAGCA GGAACCACAG GAGGATGGCC AGGCTCAACC GCCCCCCAAA 
ACTCCAAAGA CCCTCAGCAG TTTCTTCTCC CCCCGGAAGC CAGCTGTCAA AAAAGAAGCG 
AAAGAAGAAG GACCTGGTGC TCTGAGAAAG GACGAGATCA AGGGACACGT GGACCCAGCG 
AGCTATAATC CTGCCAAGAA CAACTATCAC CCCATCGAAG ATGCCTGCTG GAAGGCGGGC 
CAGAGGGTTC CTTATCTGGC TGTGGCCCGG GCGTTCGAGA AGATCGAGGA GGTCTCTGCT 
CGGCTCCGGA TGGTGGAGAC GCTGAGCAAC CTGCTGCGCT CCGTGGTGGC CCTGTCACCT 
GCTGACCTCC TGCCCGTCCT CTACCTGAGC CTCAACCGCC TGGGGCCGCC CCAGCAGGGC 
CTGGAGCTCG GCATCGGAGA GGGCATCCTC CTCAAGGCAG TGGCCCAGGC CACGGGTCGG 
CAGCTGGAGT CCGTCCGGGC CGAGGTGGCC GAGAAGGGTG ATGTGGGGCT GGTGGCCGAG 
AGCAGCCGCA GCACCCAGAG GCTCGTGCTG CCCCCGCCTG CGCTCACTGC CGCCGGCGTC 
TTCGCCAAGT TCCGGGACAT CGCGCAGCTG GCAGGCAGCG CTTCCACAAC CAAGAAGATT 
GACGTCATCA AAGGCCTCTT TGTGGCCTGC CGTCACTCAG AGGCACGTTT CATTGCCAGA 
GCCCTGAGTG GACGGCTGCG CCTCGGGTTG GCTGAGCAGT CGGTGCTGGC GGCCCTCGCC 
CAGGCCGTGA GCCTCACGCC CCCCAGTCAA GAATGCCCCC CCGCCGTGGT GGACGCCGGG 
AAGGGCAAGA CGGCAGAAGC CAGAAAGACG TGGCTGGAGG AGCAAGGCAT GATCCTGAAG 
CAGACTTTCT GTGAGGTACC TGACCTGGAG CGGATCGTCC CCGTGCTTCT GGAACACGGC 
CTGGAGCGGC TCCCGGAGCA CTGCAGACTG AGCCCAGGGG TTCCCCTGAA ACCAATGTTG 
GCCCACCCCA CCCGGGGTGT CAGTGAGGTC CTGAAGCGCT TTGAGGAGGC AGCTTTCACC 
TGCGAGTACA AATACGACGG GCAGAGGGCA CAGATCCACG TTCTGGAAGG CGGGGAGGTA 
AAGATCTTCA GCAGGAATCA GGAGGACAAT ACTGGAAGGT ACCCCGACAT TATCAGCCGC 
ATCCCTAAGA TTAAACTCCC ATCGGTCACA TCCTTCATCC TAGACACAGA AGCTGTAGCT 
TGGGACCGGG AAAAGAAGCA GATCCAGCCA TTCCAAGTCC TCACCACCCG CAAACGCAAG 
GAGGTGGACG CGGCGGAGAT CCAGGTGCAG GTGTGTCTGT ATGCCTTCGA TCTCATTTAC 
CTCAACGGAA AGTCCCTGGT ACGTGAGCCC CTCTCCCGAC GCCGGCAGCT GCTCCGGGAG 
AACTTCGTGG AGACGGAGGG CGAGTTCGTC TTTGCCACCT CCCTAGACAC CAAGGACACG 
GAGCAGATCG CAGAGTTCTT GGAGCAGTCG GTGAAAGACT CCTGCGAGGG GCTGATGGTG 
AAGACCCTGG ACGTGGACGC CACCTACGAG ATCGCCAAGA GGTCACACAA CTGGCTCAAG 
CTGAAGAAGG ACTACCTCGA GGGTGTGGGC GACACCCTGG ACCTCGTAGT GATTGGTGCC 
TACCTGGGCA AGGGGAAACG GGCCGGCCGG TACGGGGGCT TCCTGCTGGC CTCCTATGAC 
GAGGAGAGCG AGGAGTTCCA GGCCATCTGC AAGCTCGGAA CTGGCTTCAG TGACGAGGAG 
CTGGAAGAGC ACCACCAGAG GCTCCAGGCC TTAGTGCTGC CCACCCCGCG CTCCTACGTT 
CGGGCTGACG GTGCGGTGGC CCCCGACCAC TGGCTGGACC CCAGCGACGT GTGGGAGGTG 
AAGTGCGCGG ACCTCTCCCT GTCCCCCATC TACCCTGCGG CCCGAGGCCT GGTGGACAGC 
GAGAAGGGGA TCTCCCTCCG CTTCCCTCGG TTTATTCGCG TCCGTGAGGA TAAGAAGCCG 
GAGCAGGCCA CTACCAGTGC CCAGGTGGCC GGTCTGTACA AGAAGCAGAG TCAGATTCAG 
AACCAGCAGG GTGCGGAGCC GGACTCCGAG CAGGAAGAGT TCTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005219188.1
CDS	172..2919
Translation	MQRSIMSFFQPKKEGKAKKPEKETAKSIRETESPPKVALKERNRVVSEGDSPVKRPGRKATRVLGSEGEEEEEEAPTTPRSQKPASDSPQASPPSPVTLPESSPSHSKPSPAVASPSGIPKRRTARKQLPKRTFQDVLKEQGEDKDPETKKKKEEEAETPIESLPEPEGDDKEEVEGGDQPTTPPEPRKTSKSLTESVSEPGVTVKQEPQEDGQAQPPPKTPKTLSSFFSPRKPAVKKEAKEEGPGALRKDEIKGHVDPASYNPAKNNYHPIEDACWKAGQRVPYLAVARAFEKIEEVSARLRMVETLSNLLRSVVALSPADLLPVLYLSLNRLGPPQQGLELGIGEGILLKAVAQATGRQLESVRAEVAEKGDVGLVAESSRSTQRLVLPPPALTAAGVFAKFRDIAQLAGSASTTKKIDVIKGLFVACRHSEARFIARALSGRLRLGLAEQSVLAALAQAVSLTPPSQECPPAVVDAGKGKTAEARKTWLEEQGMILKQTFCEVPDLERIVPVLLEHGLERLPEHCRLSPGVPLKPMLAHPTRGVSEVLKRFEEAAFTCEYKYDGQRAQIHVLEGGEVKIFSRNQEDNTGRYPDIISRIPKIKLPSVTSFILDTEAVAWDREKKQIQPFQVLTTRKRKEVDAAEIQVQVCLYAFDLIYLNGKSLVREPLSRRRQLLRENFVETEGEFVFATSLDTKDTEQIAEFLEQSVKDSCEGLMVKTLDVDATYEIAKRSHNWLKLKKDYLEGVGDTLDLVVIGAYLGKGKRAGRYGGFLLASYDEESEEFQAICKLGTGFSDEELEEHHQRLQALVLPTPRSYVRADGAVAPDHWLDPSDVWEVKCADLSLSPIYPAARGLVDSEKGISLRFPRFIRVREDKKPEQATTSAQVAGLYKKQSQIQNQQGAEPDSEQEEFY

Target ORF information:

RefSeq Version	XM_005219131.3
Organism	Bos taurus(cattle)
Definition	Bos taurus ligase I, DNA, ATP-dependent (LIG1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005219131.3

ORF Insert Sequence:

ATGCAGCGAA GTATCATGTC ATTTTTCCAG CCCAAGAAAG AGGGCAAAGC GAAGAAGCCA 
GAGAAGGAGA CAGCCAAGAG CATCAGAGAA ACAGAGTCCC CTCCGAAGGT GGCGCTGAAG 
GAGCGGAATC GAGTGGTGTC TGAGGGTGAC TCTCCAGTGA AGAGGCCAGG GAGGAAGGCG 
ACCCGGGTCC TGGGCAGCGA GGGCGAGGAG GAGGAAGAGG AAGCCCCCAC CACCCCCAGA 
AGCCAGAAGC CTGCTTCAGA CTCTCCACAG GCCTCGCCTC CCAGTCCTGT CACACTGCCT 
GAGAGCAGCC CTTCCCACTC CAAACCCTCC CCAGCAGTTG CATCCCCATC AGGGATCCCG 
AAGCGTCGCA CGGCCCGGAA GCAGCTTCCC AAACGGACAT TTCAAGATGT TCTGAAAGAG 
CAGGGCGAGG ACAAGGATCC AGAAACTAAG AAGAAGAAGG AGGAAGAAGC GGAGACACCA 
ATAGAAAGCC TCCCAGAGCC TGAAGGGGAC GACAAGGAGG AAGTGGAAGG TGGGGACCAG 
CCCACGACGC CCCCTGAGCC CCGGAAGACC TCCAAGTCCC TGACAGAAAG CGTCTCAGAG 
CCCGGGGTGA CGGTGAAGCA GGAACCACAG GAGGATGGCC AGGCTCAACC GCCCCCCAAA 
ACTCCAAAGA CCCTCAGCAG TTTCTTCTCC CCCCGGAAGC CAGCTGTCAA AAAAGAAGCG 
AAAGAAGAAG GACCTGGTGC TCTGAGAAAG GACGAGATCA AGGGACACGT GGACCCAGCG 
AGCTATAATC CTGCCAAGAA CAACTATCAC CCCATCGAAG ATGCCTGCTG GAAGGCGGGC 
CAGAGGGTTC CTTATCTGGC TGTGGCCCGG GCGTTCGAGA AGATCGAGGA GGTCTCTGCT 
CGGCTCCGGA TGGTGGAGAC GCTGAGCAAC CTGCTGCGCT CCGTGGTGGC CCTGTCACCT 
GCTGACCTCC TGCCCGTCCT CTACCTGAGC CTCAACCGCC TGGGGCCGCC CCAGCAGGGC 
CTGGAGCTCG GCATCGGAGA GGGCATCCTC CTCAAGGCAG TGGCCCAGGC CACGGGTCGG 
CAGCTGGAGT CCGTCCGGGC CGAGGTGGCC GAGAAGGGTG ATGTGGGGCT GGTGGCCGAG 
AGCAGCCGCA GCACCCAGAG GCTCGTGCTG CCCCCGCCTG CGCTCACTGC CGCCGGCGTC 
TTCGCCAAGT TCCGGGACAT CGCGCAGCTG GCAGGCAGCG CTTCCACAAC CAAGAAGATT 
GACGTCATCA AAGGCCTCTT TGTGGCCTGC CGTCACTCAG AGGCACGTTT CATTGCCAGA 
GCCCTGAGTG GACGGCTGCG CCTCGGGTTG GCTGAGCAGT CGGTGCTGGC GGCCCTCGCC 
CAGGCCGTGA GCCTCACGCC CCCCAGTCAA GAATGCCCCC CCGCCGTGGT GGACGCCGGG 
AAGGGCAAGA CGGCAGAAGC CAGAAAGACG TGGCTGGAGG AGCAAGGCAT GATCCTGAAG 
CAGACTTTCT GTGAGGTACC TGACCTGGAG CGGATCGTCC CCGTGCTTCT GGAACACGGC 
CTGGAGCGGC TCCCGGAGCA CTGCAGACTG AGCCCAGGGG TTCCCCTGAA ACCAATGTTG 
GCCCACCCCA CCCGGGGTGT CAGTGAGGTC CTGAAGCGCT TTGAGGAGGC AGCTTTCACC 
TGCGAGTACA AATACGACGG GCAGAGGGCA CAGATCCACG TTCTGGAAGG CGGGGAGGTA 
AAGATCTTCA GCAGGAATCA GGAGGACAAT ACTGGAAGGT ACCCCGACAT TATCAGCCGC 
ATCCCTAAGA TTAAACTCCC ATCGGTCACA TCCTTCATCC TAGACACAGA AGCTGTAGCT 
TGGGACCGGG AAAAGAAGCA GATCCAGCCA TTCCAAGTCC TCACCACCCG CAAACGCAAG 
GAGGTGGACG CGGCGGAGAT CCAGGTGCAG GTGTGTCTGT ATGCCTTCGA TCTCATTTAC 
CTCAACGGAA AGTCCCTGGT ACGTGAGCCC CTCTCCCGAC GCCGGCAGCT GCTCCGGGAG 
AACTTCGTGG AGACGGAGGG CGAGTTCGTC TTTGCCACCT CCCTAGACAC CAAGGACACG 
GAGCAGATCG CAGAGTTCTT GGAGCAGTCG GTGAAAGACT CCTGCGAGGG GCTGATGGTG 
AAGACCCTGG ACGTGGACGC CACCTACGAG ATCGCCAAGA GGTCACACAA CTGGCTCAAG 
CTGAAGAAGG ACTACCTCGA GGGTGTGGGC GACACCCTGG ACCTCGTAGT GATTGGTGCC 
TACCTGGGCA AGGGGAAACG GGCCGGCCGG TACGGGGGCT TCCTGCTGGC CTCCTATGAC 
GAGGAGAGCG AGGAGTTCCA GGCCATCTGC AAGCTCGGAA CTGGCTTCAG TGACGAGGAG 
CTGGAAGAGC ACCACCAGAG GCTCCAGGCC TTAGTGCTGC CCACCCCGCG CTCCTACGTT 
CGGGCTGACG GTGCGGTGGC CCCCGACCAC TGGCTGGACC CCAGCGACGT GTGGGAGGTG 
AAGTGCGCGG ACCTCTCCCT GTCCCCCATC TACCCTGCGG CCCGAGGCCT GGTGGACAGC 
GAGAAGGGGA TCTCCCTCCG CTTCCCTCGG TTTATTCGCG TCCGTGAGGA TAAGAAGCCG 
GAGCAGGCCA CTACCAGTGC CCAGGTGGCC GGTCTGTACA AGAAGCAGAG TCAGATTCAG 
AACCAGCAGG GTGCGGAGCC GGACTCCGAG CAGGAAGAGT TCTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIG1 ( XM_010814980.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010813282.1 Bos taurus ligase I, DNA, ATP-dependent (LIG1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa58683
Clone ID Related Accession (Same CDS sequence)	XM_010814980.2
Accession Version	XM_010814980.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2796bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	DNA ligase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104466.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010814980.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCGAA GTATCATGTC ATTTTTCCAG CCCAAGAAAG AGGGCAAAGC GAAGAAGCCA 
GAGAAGGAGA CAGCCAAGAG CATCAGAGAA ACAGAGTCCC CTCCGAAGGT GGCGCTGAAG 
GAGCGGAATC GAGTGGTGTC TGAGGGTGAC TCTCCAGTGA AGAGGCCAGG GAGGAAGGCG 
ACCCGGGTCC TGGGCAGCGA GGGCGAGGAG GAGGAAGAGG AAGCCCCCAC CACCCCCAGA 
AGCCAGAAGC CTGCTTCAGA CTCTCCACAG GCCTCGCCTC CCAGTCCTGT CACACTGCCT 
GAGAGCAGCC CTTCCCACTC CAAACCCTCC CCAGCAGTTG CATCCCCATC AGGGATCCCG 
AAGCGTCGCA CGGCCCGGAA GCAGCTTCCC AAACGGACAT TTCAAGATGT TCTGAAAGAG 
CAGGGCGAGG ACAAGGATCC AGAAACTAAG AAGAAGAAGG AGGAAGAAGC GGAGACACCA 
ATAGAAAGCC TCCCAGAGCC TGAAGGGGAC GACAAGGAGG AAGTGGAAGG TGGGGACCAG 
CCCACGACGC CCCCTGAGCC CCGGAAGACC TCCACAGAGT CCCTGACAGA AAGCGTCTCA 
GAGCCCGGGG TGACGGTGAA GCAGGAACCA CAGGAGGATG GCCAGGCTCA ACCGCCCCCC 
AAAACTCCAA AGACCCTCAG CAGTTTCTTC TGTGAGTGGG GACTTCACCT TGCGGGGCTG 
CGCAGGGAAG AGGCAGCCCC CCGGAAGCCA GCTGTCAAAA AAGAAGCGAA AGAAGAAGGA 
CCTGGTGCTC TGAGAAAGGA CGAGATCAAG GGACACGTGG ACCCAGCGAG CTATAATCCT 
GCCAAGAACA ACTATCACCC CATCGAAGAT GCCTGCTGGA AGGCGGGCCA GAGGGTTCCT 
TATCTGGCTG TGGCCCGGGC GTTCGAGAAG ATCGAGGAGG TCTCTGCTCG GCTCCGGATG 
GTGGAGACGC TGAGCAACCT GCTGCGCTCC GTGGTGGCCC TGTCACCTGC TGACCTCCTG 
CCCGTCCTCT ACCTGAGCCT CAACCGCCTG GGGCCGCCCC AGCAGGGCCT GGAGCTCGGC 
ATCGGAGAGG GCATCCTCCT CAAGGCAGTG GCCCAGGCCA CGGGTCGGCA GCTGGAGTCC 
GTCCGGGCCG AGGTGGCCGA GAAGGGTGAT GTGGGGCTGG TGGCCGAGAG CAGCCGCAGC 
ACCCAGAGGC TCGTGCTGCC CCCGCCTGCG CTCACTGCCG CCGGCGTCTT CGCCAAGTTC 
CGGGACATCG CGCAGCTGGC AGGCAGCGCT TCCACAACCA AGAAGATTGA CGTCATCAAA 
GGCCTCTTTG TGGCCTGCCG TCACTCAGAG GCACGTTTCA TTGCCAGAGC CCTGAGTGGA 
CGGCTGCGCC TCGGGTTGGC TGAGCAGTCG GTGCTGGCGG CCCTCGCCCA GGCCGTGAGC 
CTCACGCCCC CCAGTCAAGA ATGCCCCCCC GCCGTGGTGG ACGCCGGGAA GGGCAAGACG 
GCAGAAGCCA GAAAGACGTG GCTGGAGGAG CAAGGCATGA TCCTGAAGCA GACTTTCTGT 
GAGGTACCTG ACCTGGAGCG GATCGTCCCC GTGCTTCTGG AACACGGCCT GGAGCGGCTC 
CCGGAGCACT GCAGACTGAG CCCAGGGGTT CCCCTGAAAC CAATGTTGGC CCACCCCACC 
CGGGGTGTCA GTGAGGTCCT GAAGCGCTTT GAGGAGGCAG CTTTCACCTG CGAGTACAAA 
TACGACGGGC AGAGGGCACA GATCCACGTT CTGGAAGGCG GGGAGGTAAA GATCTTCAGC 
AGGAATCAGG AGGACAATAC TGGAAGGTAC CCCGACATTA TCAGCCGCAT CCCTAAGATT 
AAACTCCCAT CGGTCACATC CTTCATCCTA GACACAGAAG CTGTAGCTTG GGACCGGGAA 
AAGAAGCAGA TCCAGCCATT CCAAGTCCTC ACCACCCGCA AACGCAAGGA GGTGGACGCG 
GCGGAGATCC AGGTGCAGGT GTGTCTGTAT GCCTTCGATC TCATTTACCT CAACGGAAAG 
TCCCTGGTAC GTGAGCCCCT CTCCCGACGC CGGCAGCTGC TCCGGGAGAA CTTCGTGGAG 
ACGGAGGGCG AGTTCGTCTT TGCCACCTCC CTAGACACCA AGGACACGGA GCAGATCGCA 
GAGTTCTTGG AGCAGTCGGT GAAAGACTCC TGCGAGGGGC TGATGGTGAA GACCCTGGAC 
GTGGACGCCA CCTACGAGAT CGCCAAGAGG TCACACAACT GGCTCAAGCT GAAGAAGGAC 
TACCTCGAGG GTGTGGGCGA CACCCTGGAC CTCGTAGTGA TTGGTGCCTA CCTGGGCAAG 
GGGAAACGGG CCGGCCGGTA CGGGGGCTTC CTGCTGGCCT CCTATGACGA GGAGAGCGAG 
GAGTTCCAGG CCATCTGCAA GCTCGGAACT GGCTTCAGTG ACGAGGAGCT GGAAGAGCAC 
CACCAGAGGC TCCAGGCCTT AGTGCTGCCC ACCCCGCGCT CCTACGTTCG GGCTGACGGT 
GCGGTGGCCC CCGACCACTG GCTGGACCCC AGCGACGTGT GGGAGGTGAA GTGCGCGGAC 
CTCTCCCTGT CCCCCATCTA CCCTGCGGCC CGAGGCCTGG TGGACAGCGA GAAGGGGATC 
TCCCTCCGCT TCCCTCGGTT TATTCGCGTC CGTGAGGATA AGAAGCCGGA GCAGGCCACT 
ACCAGTGCCC AGGTGGCCGG TCTGTACAAG AAGCAGAGTC AGATTCAGAA CCAGCAGGGT 
GCGGAGCCGG ACTCCGAGCA GGAAGAGTTC TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010813282.1
CDS	172..2967
Translation	MQRSIMSFFQPKKEGKAKKPEKETAKSIRETESPPKVALKERNRVVSEGDSPVKRPGRKATRVLGSEGEEEEEEAPTTPRSQKPASDSPQASPPSPVTLPESSPSHSKPSPAVASPSGIPKRRTARKQLPKRTFQDVLKEQGEDKDPETKKKKEEEAETPIESLPEPEGDDKEEVEGGDQPTTPPEPRKTSTESLTESVSEPGVTVKQEPQEDGQAQPPPKTPKTLSSFFCEWGLHLAGLRREEAAPRKPAVKKEAKEEGPGALRKDEIKGHVDPASYNPAKNNYHPIEDACWKAGQRVPYLAVARAFEKIEEVSARLRMVETLSNLLRSVVALSPADLLPVLYLSLNRLGPPQQGLELGIGEGILLKAVAQATGRQLESVRAEVAEKGDVGLVAESSRSTQRLVLPPPALTAAGVFAKFRDIAQLAGSASTTKKIDVIKGLFVACRHSEARFIARALSGRLRLGLAEQSVLAALAQAVSLTPPSQECPPAVVDAGKGKTAEARKTWLEEQGMILKQTFCEVPDLERIVPVLLEHGLERLPEHCRLSPGVPLKPMLAHPTRGVSEVLKRFEEAAFTCEYKYDGQRAQIHVLEGGEVKIFSRNQEDNTGRYPDIISRIPKIKLPSVTSFILDTEAVAWDREKKQIQPFQVLTTRKRKEVDAAEIQVQVCLYAFDLIYLNGKSLVREPLSRRRQLLRENFVETEGEFVFATSLDTKDTEQIAEFLEQSVKDSCEGLMVKTLDVDATYEIAKRSHNWLKLKKDYLEGVGDTLDLVVIGAYLGKGKRAGRYGGFLLASYDEESEEFQAICKLGTGFSDEELEEHHQRLQALVLPTPRSYVRADGAVAPDHWLDPSDVWEVKCADLSLSPIYPAARGLVDSEKGISLRFPRFIRVREDKKPEQATTSAQVAGLYKKQSQIQNQQGAEPDSEQEEFY

Target ORF information:

RefSeq Version	XM_010814980.2
Organism	Bos taurus(cattle)
Definition	Bos taurus ligase I, DNA, ATP-dependent (LIG1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010814980.2

ORF Insert Sequence:

ATGCAGCGAA GTATCATGTC ATTTTTCCAG CCCAAGAAAG AGGGCAAAGC GAAGAAGCCA 
GAGAAGGAGA CAGCCAAGAG CATCAGAGAA ACAGAGTCCC CTCCGAAGGT GGCGCTGAAG 
GAGCGGAATC GAGTGGTGTC TGAGGGTGAC TCTCCAGTGA AGAGGCCAGG GAGGAAGGCG 
ACCCGGGTCC TGGGCAGCGA GGGCGAGGAG GAGGAAGAGG AAGCCCCCAC CACCCCCAGA 
AGCCAGAAGC CTGCTTCAGA CTCTCCACAG GCCTCGCCTC CCAGTCCTGT CACACTGCCT 
GAGAGCAGCC CTTCCCACTC CAAACCCTCC CCAGCAGTTG CATCCCCATC AGGGATCCCG 
AAGCGTCGCA CGGCCCGGAA GCAGCTTCCC AAACGGACAT TTCAAGATGT TCTGAAAGAG 
CAGGGCGAGG ACAAGGATCC AGAAACTAAG AAGAAGAAGG AGGAAGAAGC GGAGACACCA 
ATAGAAAGCC TCCCAGAGCC TGAAGGGGAC GACAAGGAGG AAGTGGAAGG TGGGGACCAG 
CCCACGACGC CCCCTGAGCC CCGGAAGACC TCCACAGAGT CCCTGACAGA AAGCGTCTCA 
GAGCCCGGGG TGACGGTGAA GCAGGAACCA CAGGAGGATG GCCAGGCTCA ACCGCCCCCC 
AAAACTCCAA AGACCCTCAG CAGTTTCTTC TGTGAGTGGG GACTTCACCT TGCGGGGCTG 
CGCAGGGAAG AGGCAGCCCC CCGGAAGCCA GCTGTCAAAA AAGAAGCGAA AGAAGAAGGA 
CCTGGTGCTC TGAGAAAGGA CGAGATCAAG GGACACGTGG ACCCAGCGAG CTATAATCCT 
GCCAAGAACA ACTATCACCC CATCGAAGAT GCCTGCTGGA AGGCGGGCCA GAGGGTTCCT 
TATCTGGCTG TGGCCCGGGC GTTCGAGAAG ATCGAGGAGG TCTCTGCTCG GCTCCGGATG 
GTGGAGACGC TGAGCAACCT GCTGCGCTCC GTGGTGGCCC TGTCACCTGC TGACCTCCTG 
CCCGTCCTCT ACCTGAGCCT CAACCGCCTG GGGCCGCCCC AGCAGGGCCT GGAGCTCGGC 
ATCGGAGAGG GCATCCTCCT CAAGGCAGTG GCCCAGGCCA CGGGTCGGCA GCTGGAGTCC 
GTCCGGGCCG AGGTGGCCGA GAAGGGTGAT GTGGGGCTGG TGGCCGAGAG CAGCCGCAGC 
ACCCAGAGGC TCGTGCTGCC CCCGCCTGCG CTCACTGCCG CCGGCGTCTT CGCCAAGTTC 
CGGGACATCG CGCAGCTGGC AGGCAGCGCT TCCACAACCA AGAAGATTGA CGTCATCAAA 
GGCCTCTTTG TGGCCTGCCG TCACTCAGAG GCACGTTTCA TTGCCAGAGC CCTGAGTGGA 
CGGCTGCGCC TCGGGTTGGC TGAGCAGTCG GTGCTGGCGG CCCTCGCCCA GGCCGTGAGC 
CTCACGCCCC CCAGTCAAGA ATGCCCCCCC GCCGTGGTGG ACGCCGGGAA GGGCAAGACG 
GCAGAAGCCA GAAAGACGTG GCTGGAGGAG CAAGGCATGA TCCTGAAGCA GACTTTCTGT 
GAGGTACCTG ACCTGGAGCG GATCGTCCCC GTGCTTCTGG AACACGGCCT GGAGCGGCTC 
CCGGAGCACT GCAGACTGAG CCCAGGGGTT CCCCTGAAAC CAATGTTGGC CCACCCCACC 
CGGGGTGTCA GTGAGGTCCT GAAGCGCTTT GAGGAGGCAG CTTTCACCTG CGAGTACAAA 
TACGACGGGC AGAGGGCACA GATCCACGTT CTGGAAGGCG GGGAGGTAAA GATCTTCAGC 
AGGAATCAGG AGGACAATAC TGGAAGGTAC CCCGACATTA TCAGCCGCAT CCCTAAGATT 
AAACTCCCAT CGGTCACATC CTTCATCCTA GACACAGAAG CTGTAGCTTG GGACCGGGAA 
AAGAAGCAGA TCCAGCCATT CCAAGTCCTC ACCACCCGCA AACGCAAGGA GGTGGACGCG 
GCGGAGATCC AGGTGCAGGT GTGTCTGTAT GCCTTCGATC TCATTTACCT CAACGGAAAG 
TCCCTGGTAC GTGAGCCCCT CTCCCGACGC CGGCAGCTGC TCCGGGAGAA CTTCGTGGAG 
ACGGAGGGCG AGTTCGTCTT TGCCACCTCC CTAGACACCA AGGACACGGA GCAGATCGCA 
GAGTTCTTGG AGCAGTCGGT GAAAGACTCC TGCGAGGGGC TGATGGTGAA GACCCTGGAC 
GTGGACGCCA CCTACGAGAT CGCCAAGAGG TCACACAACT GGCTCAAGCT GAAGAAGGAC 
TACCTCGAGG GTGTGGGCGA CACCCTGGAC CTCGTAGTGA TTGGTGCCTA CCTGGGCAAG 
GGGAAACGGG CCGGCCGGTA CGGGGGCTTC CTGCTGGCCT CCTATGACGA GGAGAGCGAG 
GAGTTCCAGG CCATCTGCAA GCTCGGAACT GGCTTCAGTG ACGAGGAGCT GGAAGAGCAC 
CACCAGAGGC TCCAGGCCTT AGTGCTGCCC ACCCCGCGCT CCTACGTTCG GGCTGACGGT 
GCGGTGGCCC CCGACCACTG GCTGGACCCC AGCGACGTGT GGGAGGTGAA GTGCGCGGAC 
CTCTCCCTGT CCCCCATCTA CCCTGCGGCC CGAGGCCTGG TGGACAGCGA GAAGGGGATC 
TCCCTCCGCT TCCCTCGGTT TATTCGCGTC CGTGAGGATA AGAAGCCGGA GCAGGCCACT 
ACCAGTGCCC AGGTGGCCGG TCTGTACAAG AAGCAGAGTC AGATTCAGAA CCAGCAGGGT 
GCGGAGCCGG ACTCCGAGCA GGAAGAGTTC TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIG1 ( XM_010814981.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010813283.1 Bos taurus ligase I, DNA, ATP-dependent (LIG1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa58684
Clone ID Related Accession (Same CDS sequence)	XM_010814981.2
Accession Version	XM_010814981.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2793bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	DNA ligase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104466.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010814981.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCGAA GTATCATGTC ATTTTTCCAG CCCAAGAAAG AGGGCAAAGC GAAGAAGCCA 
GAGAAGGAGA CAGCCAAGAG CATCAGAGAA ACAGAGTCCC CTCCGAAGGT GGCGCTGAAG 
GAGCGGAATC GAGTGGTGTC TGAGGGTGAC TCTCCAGTGA AGAGGCCAGG GAGGAAGGCG 
ACCCGGGTCC TGGGCAGCGA GGGCGAGGAG GAGGAAGAGG AAGCCCCCAC CACCCCCAGA 
AGCCAGAAGC CTGCTTCAGA CTCTCCACAG GCCTCGCCTC CCAGTCCTGT CACACTGCCT 
GAGAGCAGCC CTTCCCACTC CAAACCCTCC CCAGCAGTTG CATCCCCATC AGGGATCCCG 
AAGCGTCGCA CGGCCCGGAA GCAGCTTCCC AAACGGACAT TTCAAGATGT TCTGAAAGAG 
CAGGGCGAGG ACAAGGATCC AGAAACTAAG AAGAAGAAGG AGGAAGAAGC GGAGACACCA 
ATAGAAAGCC TCCCAGAGCC TGAAGGGGAC GACAAGGAGG AAGTGGAAGG TGGGGACCAG 
CCCACGACGC CCCCTGAGCC CCGGAAGACC TCCAAGTCCC TGACAGAAAG CGTCTCAGAG 
CCCGGGGTGA CGGTGAAGCA GGAACCACAG GAGGATGGCC AGGCTCAACC GCCCCCCAAA 
ACTCCAAAGA CCCTCAGCAG TTTCTTCTGT GAGTGGGGAC TTCACCTTGC GGGGCTGCGC 
AGGGAAGAGG CAGCCCCCCG GAAGCCAGCT GTCAAAAAAG AAGCGAAAGA AGAAGGACCT 
GGTGCTCTGA GAAAGGACGA GATCAAGGGA CACGTGGACC CAGCGAGCTA TAATCCTGCC 
AAGAACAACT ATCACCCCAT CGAAGATGCC TGCTGGAAGG CGGGCCAGAG GGTTCCTTAT 
CTGGCTGTGG CCCGGGCGTT CGAGAAGATC GAGGAGGTCT CTGCTCGGCT CCGGATGGTG 
GAGACGCTGA GCAACCTGCT GCGCTCCGTG GTGGCCCTGT CACCTGCTGA CCTCCTGCCC 
GTCCTCTACC TGAGCCTCAA CCGCCTGGGG CCGCCCCAGC AGGGCCTGGA GCTCGGCATC 
GGAGAGGGCA TCCTCCTCAA GGCAGTGGCC CAGGCCACGG GTCGGCAGCT GGAGTCCGTC 
CGGGCCGAGG TGGCCGAGAA GGGTGATGTG GGGCTGGTGG CCGAGAGCAG CCGCAGCACC 
CAGAGGCTCG TGCTGCCCCC GCCTGCGCTC ACTGCCGCCG GCGTCTTCGC CAAGTTCCGG 
GACATCGCGC AGCTGGCAGG CAGCGCTTCC ACAACCAAGA AGATTGACGT CATCAAAGGC 
CTCTTTGTGG CCTGCCGTCA CTCAGAGGCA CGTTTCATTG CCAGAGCCCT GAGTGGACGG 
CTGCGCCTCG GGTTGGCTGA GCAGTCGGTG CTGGCGGCCC TCGCCCAGGC CGTGAGCCTC 
ACGCCCCCCA GTCAAGAATG CCCCCCCGCC GTGGTGGACG CCGGGAAGGG CAAGACGGCA 
GAAGCCAGAA AGACGTGGCT GGAGGAGCAA GGCATGATCC TGAAGCAGAC TTTCTGTGAG 
GTACCTGACC TGGAGCGGAT CGTCCCCGTG CTTCTGGAAC ACGGCCTGGA GCGGCTCCCG 
GAGCACTGCA GACTGAGCCC AGGGGTTCCC CTGAAACCAA TGTTGGCCCA CCCCACCCGG 
GGTGTCAGTG AGGTCCTGAA GCGCTTTGAG GAGGCAGCTT TCACCTGCGA GTACAAATAC 
GACGGGCAGA GGGCACAGAT CCACGTTCTG GAAGGCGGGG AGGTAAAGAT CTTCAGCAGG 
AATCAGGAGG ACAATACTGG AAGGTACCCC GACATTATCA GCCGCATCCC TAAGATTAAA 
CTCCCATCGG TCACATCCTT CATCCTAGAC ACAGAAGCTG TAGCTTGGGA CCGGGAAAAG 
AAGCAGATCC AGCCATTCCA AGTCCTCACC ACCCGCAAAC GCAAGGAGGT GGACGCGGCG 
GAGATCCAGG TGCAGGTGTG TCTGTATGCC TTCGATCTCA TTTACCTCAA CGGAAAGTCC 
CTGGTACGTG AGCCCCTCTC CCGACGCCGG CAGCTGCTCC GGGAGAACTT CGTGGAGACG 
GAGGGCGAGT TCGTCTTTGC CACCTCCCTA GACACCAAGG ACACGGAGCA GATCGCAGAG 
TTCTTGGAGC AGTCGGTGAA AGACTCCTGC GAGGGGCTGA TGGTGAAGAC CCTGGACGTG 
GACGCCACCT ACGAGATCGC CAAGAGGTCA CACAACTGGC TCAAGCTGAA GAAGGACTAC 
CTCGAGGGTG TGGGCGACAC CCTGGACCTC GTAGTGATTG GTGCCTACCT GGGCAAGGGG 
AAACGGGCCG GCCGGTACGG GGGCTTCCTG CTGGCCTCCT ATGACGAGGA GAGCGAGGAG 
TTCCAGGCCA TCTGCAAGCT CGGAACTGGC TTCAGTGACG AGGAGCTGGA AGAGCACCAC 
CAGAGGCTCC AGGCCTTAGT GCTGCCCACC CCGCGCTCCT ACGTTCGGGC TGACGGTGCG 
GTGGCCCCCG ACCACTGGCT GGACCCCAGC GACGTGTGGG AGGTGAAGTG CGCGGACCTC 
TCCCTGTCCC CCATCTACCC TGCGGCCCGA GGCCTGGTGG ACAGCGAGAA GGGGATCTCC 
CTCCGCTTCC CTCGGTTTAT TCGCGTCCGT GAGGATAAGA AGCCGGAGCA GGCCACTACC 
AGTGCCCAGG TGGCCGGTCT GTACAAGAAG CAGAGTCAGA TTCAGAACCA GCAGGGTGCG 
GAGCCGGACT CCGAGCAGGA AGAGTTCTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010813283.1
CDS	172..2964
Translation	MQRSIMSFFQPKKEGKAKKPEKETAKSIRETESPPKVALKERNRVVSEGDSPVKRPGRKATRVLGSEGEEEEEEAPTTPRSQKPASDSPQASPPSPVTLPESSPSHSKPSPAVASPSGIPKRRTARKQLPKRTFQDVLKEQGEDKDPETKKKKEEEAETPIESLPEPEGDDKEEVEGGDQPTTPPEPRKTSKSLTESVSEPGVTVKQEPQEDGQAQPPPKTPKTLSSFFCEWGLHLAGLRREEAAPRKPAVKKEAKEEGPGALRKDEIKGHVDPASYNPAKNNYHPIEDACWKAGQRVPYLAVARAFEKIEEVSARLRMVETLSNLLRSVVALSPADLLPVLYLSLNRLGPPQQGLELGIGEGILLKAVAQATGRQLESVRAEVAEKGDVGLVAESSRSTQRLVLPPPALTAAGVFAKFRDIAQLAGSASTTKKIDVIKGLFVACRHSEARFIARALSGRLRLGLAEQSVLAALAQAVSLTPPSQECPPAVVDAGKGKTAEARKTWLEEQGMILKQTFCEVPDLERIVPVLLEHGLERLPEHCRLSPGVPLKPMLAHPTRGVSEVLKRFEEAAFTCEYKYDGQRAQIHVLEGGEVKIFSRNQEDNTGRYPDIISRIPKIKLPSVTSFILDTEAVAWDREKKQIQPFQVLTTRKRKEVDAAEIQVQVCLYAFDLIYLNGKSLVREPLSRRRQLLRENFVETEGEFVFATSLDTKDTEQIAEFLEQSVKDSCEGLMVKTLDVDATYEIAKRSHNWLKLKKDYLEGVGDTLDLVVIGAYLGKGKRAGRYGGFLLASYDEESEEFQAICKLGTGFSDEELEEHHQRLQALVLPTPRSYVRADGAVAPDHWLDPSDVWEVKCADLSLSPIYPAARGLVDSEKGISLRFPRFIRVREDKKPEQATTSAQVAGLYKKQSQIQNQQGAEPDSEQEEFY

Target ORF information:

RefSeq Version	XM_010814981.2
Organism	Bos taurus(cattle)
Definition	Bos taurus ligase I, DNA, ATP-dependent (LIG1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010814981.2

ORF Insert Sequence:

ATGCAGCGAA GTATCATGTC ATTTTTCCAG CCCAAGAAAG AGGGCAAAGC GAAGAAGCCA 
GAGAAGGAGA CAGCCAAGAG CATCAGAGAA ACAGAGTCCC CTCCGAAGGT GGCGCTGAAG 
GAGCGGAATC GAGTGGTGTC TGAGGGTGAC TCTCCAGTGA AGAGGCCAGG GAGGAAGGCG 
ACCCGGGTCC TGGGCAGCGA GGGCGAGGAG GAGGAAGAGG AAGCCCCCAC CACCCCCAGA 
AGCCAGAAGC CTGCTTCAGA CTCTCCACAG GCCTCGCCTC CCAGTCCTGT CACACTGCCT 
GAGAGCAGCC CTTCCCACTC CAAACCCTCC CCAGCAGTTG CATCCCCATC AGGGATCCCG 
AAGCGTCGCA CGGCCCGGAA GCAGCTTCCC AAACGGACAT TTCAAGATGT TCTGAAAGAG 
CAGGGCGAGG ACAAGGATCC AGAAACTAAG AAGAAGAAGG AGGAAGAAGC GGAGACACCA 
ATAGAAAGCC TCCCAGAGCC TGAAGGGGAC GACAAGGAGG AAGTGGAAGG TGGGGACCAG 
CCCACGACGC CCCCTGAGCC CCGGAAGACC TCCAAGTCCC TGACAGAAAG CGTCTCAGAG 
CCCGGGGTGA CGGTGAAGCA GGAACCACAG GAGGATGGCC AGGCTCAACC GCCCCCCAAA 
ACTCCAAAGA CCCTCAGCAG TTTCTTCTGT GAGTGGGGAC TTCACCTTGC GGGGCTGCGC 
AGGGAAGAGG CAGCCCCCCG GAAGCCAGCT GTCAAAAAAG AAGCGAAAGA AGAAGGACCT 
GGTGCTCTGA GAAAGGACGA GATCAAGGGA CACGTGGACC CAGCGAGCTA TAATCCTGCC 
AAGAACAACT ATCACCCCAT CGAAGATGCC TGCTGGAAGG CGGGCCAGAG GGTTCCTTAT 
CTGGCTGTGG CCCGGGCGTT CGAGAAGATC GAGGAGGTCT CTGCTCGGCT CCGGATGGTG 
GAGACGCTGA GCAACCTGCT GCGCTCCGTG GTGGCCCTGT CACCTGCTGA CCTCCTGCCC 
GTCCTCTACC TGAGCCTCAA CCGCCTGGGG CCGCCCCAGC AGGGCCTGGA GCTCGGCATC 
GGAGAGGGCA TCCTCCTCAA GGCAGTGGCC CAGGCCACGG GTCGGCAGCT GGAGTCCGTC 
CGGGCCGAGG TGGCCGAGAA GGGTGATGTG GGGCTGGTGG CCGAGAGCAG CCGCAGCACC 
CAGAGGCTCG TGCTGCCCCC GCCTGCGCTC ACTGCCGCCG GCGTCTTCGC CAAGTTCCGG 
GACATCGCGC AGCTGGCAGG CAGCGCTTCC ACAACCAAGA AGATTGACGT CATCAAAGGC 
CTCTTTGTGG CCTGCCGTCA CTCAGAGGCA CGTTTCATTG CCAGAGCCCT GAGTGGACGG 
CTGCGCCTCG GGTTGGCTGA GCAGTCGGTG CTGGCGGCCC TCGCCCAGGC CGTGAGCCTC 
ACGCCCCCCA GTCAAGAATG CCCCCCCGCC GTGGTGGACG CCGGGAAGGG CAAGACGGCA 
GAAGCCAGAA AGACGTGGCT GGAGGAGCAA GGCATGATCC TGAAGCAGAC TTTCTGTGAG 
GTACCTGACC TGGAGCGGAT CGTCCCCGTG CTTCTGGAAC ACGGCCTGGA GCGGCTCCCG 
GAGCACTGCA GACTGAGCCC AGGGGTTCCC CTGAAACCAA TGTTGGCCCA CCCCACCCGG 
GGTGTCAGTG AGGTCCTGAA GCGCTTTGAG GAGGCAGCTT TCACCTGCGA GTACAAATAC 
GACGGGCAGA GGGCACAGAT CCACGTTCTG GAAGGCGGGG AGGTAAAGAT CTTCAGCAGG 
AATCAGGAGG ACAATACTGG AAGGTACCCC GACATTATCA GCCGCATCCC TAAGATTAAA 
CTCCCATCGG TCACATCCTT CATCCTAGAC ACAGAAGCTG TAGCTTGGGA CCGGGAAAAG 
AAGCAGATCC AGCCATTCCA AGTCCTCACC ACCCGCAAAC GCAAGGAGGT GGACGCGGCG 
GAGATCCAGG TGCAGGTGTG TCTGTATGCC TTCGATCTCA TTTACCTCAA CGGAAAGTCC 
CTGGTACGTG AGCCCCTCTC CCGACGCCGG CAGCTGCTCC GGGAGAACTT CGTGGAGACG 
GAGGGCGAGT TCGTCTTTGC CACCTCCCTA GACACCAAGG ACACGGAGCA GATCGCAGAG 
TTCTTGGAGC AGTCGGTGAA AGACTCCTGC GAGGGGCTGA TGGTGAAGAC CCTGGACGTG 
GACGCCACCT ACGAGATCGC CAAGAGGTCA CACAACTGGC TCAAGCTGAA GAAGGACTAC 
CTCGAGGGTG TGGGCGACAC CCTGGACCTC GTAGTGATTG GTGCCTACCT GGGCAAGGGG 
AAACGGGCCG GCCGGTACGG GGGCTTCCTG CTGGCCTCCT ATGACGAGGA GAGCGAGGAG 
TTCCAGGCCA TCTGCAAGCT CGGAACTGGC TTCAGTGACG AGGAGCTGGA AGAGCACCAC 
CAGAGGCTCC AGGCCTTAGT GCTGCCCACC CCGCGCTCCT ACGTTCGGGC TGACGGTGCG 
GTGGCCCCCG ACCACTGGCT GGACCCCAGC GACGTGTGGG AGGTGAAGTG CGCGGACCTC 
TCCCTGTCCC CCATCTACCC TGCGGCCCGA GGCCTGGTGG ACAGCGAGAA GGGGATCTCC 
CTCCGCTTCC CTCGGTTTAT TCGCGTCCGT GAGGATAAGA AGCCGGAGCA GGCCACTACC 
AGTGCCCAGG TGGCCGGTCT GTACAAGAAG CAGAGTCAGA TTCAGAACCA GCAGGGTGCG 
GAGCCGGACT CCGAGCAGGA AGAGTTCTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIG1 ( XM_010814982.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010813284.1 Bos taurus ligase I, DNA, ATP-dependent (LIG1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa58685
Clone ID Related Accession (Same CDS sequence)	XM_010814982.2
Accession Version	XM_010814982.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2922bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	DNA ligase 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104466.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010814982.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGGC TTGGACCGCA ACCCGAAGGA CTCCTGGTGA CCAGTCCCGC TCTACTCTGC 
GGTTGCAGGA ACAGGAAACT GGTGACCAGC GGAGAGGAGG AGTGGGGAGA GAATTCTGAT 
ACCATCATGC AGCGAAGTAT CATGTCATTT TTCCAGCCCA AGAAAGAGGG CAAAGCGAAG 
AAGCCAGAGA AGGAGACAGC CAAGAGCATC AGAGAAACAG AGTCCCCTCC GAAGGTGGCG 
CTGAAGGAGC GGAATCGAGT GGTGTCTGAG GGTGACTCTC CAGTGAAGAG GCCAGGGAGG 
AAGGCGACCC GGGTCCTGGG CAGCGAGGGC GAGGAGGAGG AAGAGGAAGC CCCCACCACC 
CCCAGAAGCC AGAAGCCTGC TTCAGACTCT CCACAGGCCT CGCCTCCCAG TCCTGTCACA 
CTGCCTGAGA GCAGCCCTTC CCACTCCAAA CCCTCCCCAG CAGTTGCATC CCCATCAGGG 
ATCCCGAAGC GTCGCACGGC CCGGAAGCAG CTTCCCAAAC GGACATTTCA AGATGTTCTG 
AAAGAGCAGG GCGAGGACAA GGATCCAGAA ACTAAGAAGA AGAAGGAGGA AGAAGCGGAG 
ACACCAATAG AAAGCCTCCC AGAGCCTGAA GGGGACGACA AGGAGGAAGT GGAAGGTGGG 
GACCAGCCCA CGACGCCCCC TGAGCCCCGG AAGACCTCCA CAGAGTCCCT GACAGAAAGC 
GTCTCAGAGC CCGGGGTGAC GGTGAAGCAG GAACCACAGG AGGATGGCCA GGCTCAACCG 
CCCCCCAAAA CTCCAAAGAC CCTCAGCAGT TTCTTCTGTG AGTGGGGACT TCACCTTGCG 
GGGCTGCGCA GGGAAGAGGC AGCCCCCCGG AAGCCAGCTG TCAAAAAAGA AGCGAAAGAA 
GAAGGACCTG GTGCTCTGAG AAAGGACGAG ATCAAGGGAC ACGTGGACCC AGCGAGCTAT 
AATCCTGCCA AGAACAACTA TCACCCCATC GAAGATGCCT GCTGGAAGGC GGGCCAGAGG 
GTTCCTTATC TGGCTGTGGC CCGGGCGTTC GAGAAGATCG AGGAGGTCTC TGCTCGGCTC 
CGGATGGTGG AGACGCTGAG CAACCTGCTG CGCTCCGTGG TGGCCCTGTC ACCTGCTGAC 
CTCCTGCCCG TCCTCTACCT GAGCCTCAAC CGCCTGGGGC CGCCCCAGCA GGGCCTGGAG 
CTCGGCATCG GAGAGGGCAT CCTCCTCAAG GCAGTGGCCC AGGCCACGGG TCGGCAGCTG 
GAGTCCGTCC GGGCCGAGGT GGCCGAGAAG GGTGATGTGG GGCTGGTGGC CGAGAGCAGC 
CGCAGCACCC AGAGGCTCGT GCTGCCCCCG CCTGCGCTCA CTGCCGCCGG CGTCTTCGCC 
AAGTTCCGGG ACATCGCGCA GCTGGCAGGC AGCGCTTCCA CAACCAAGAA GATTGACGTC 
ATCAAAGGCC TCTTTGTGGC CTGCCGTCAC TCAGAGGCAC GTTTCATTGC CAGAGCCCTG 
AGTGGACGGC TGCGCCTCGG GTTGGCTGAG CAGTCGGTGC TGGCGGCCCT CGCCCAGGCC 
GTGAGCCTCA CGCCCCCCAG TCAAGAATGC CCCCCCGCCG TGGTGGACGC CGGGAAGGGC 
AAGACGGCAG AAGCCAGAAA GACGTGGCTG GAGGAGCAAG GCATGATCCT GAAGCAGACT 
TTCTGTGAGG TACCTGACCT GGAGCGGATC GTCCCCGTGC TTCTGGAACA CGGCCTGGAG 
CGGCTCCCGG AGCACTGCAG ACTGAGCCCA GGGGTTCCCC TGAAACCAAT GTTGGCCCAC 
CCCACCCGGG GTGTCAGTGA GGTCCTGAAG CGCTTTGAGG AGGCAGCTTT CACCTGCGAG 
TACAAATACG ACGGGCAGAG GGCACAGATC CACGTTCTGG AAGGCGGGGA GGTAAAGATC 
TTCAGCAGGA ATCAGGAGGA CAATACTGGA AGGTACCCCG ACATTATCAG CCGCATCCCT 
AAGATTAAAC TCCCATCGGT CACATCCTTC ATCCTAGACA CAGAAGCTGT AGCTTGGGAC 
CGGGAAAAGA AGCAGATCCA GCCATTCCAA GTCCTCACCA CCCGCAAACG CAAGGAGGTG 
GACGCGGCGG AGATCCAGGT GCAGGTGTGT CTGTATGCCT TCGATCTCAT TTACCTCAAC 
GGAAAGTCCC TGGTACGTGA GCCCCTCTCC CGACGCCGGC AGCTGCTCCG GGAGAACTTC 
GTGGAGACGG AGGGCGAGTT CGTCTTTGCC ACCTCCCTAG ACACCAAGGA CACGGAGCAG 
ATCGCAGAGT TCTTGGAGCA GTCGGTGAAA GACTCCTGCG AGGGGCTGAT GGTGAAGACC 
CTGGACGTGG ACGCCACCTA CGAGATCGCC AAGAGGTCAC ACAACTGGCT CAAGCTGAAG 
AAGGACTACC TCGAGGGTGT GGGCGACACC CTGGACCTCG TAGTGATTGG TGCCTACCTG 
GGCAAGGGGA AACGGGCCGG CCGGTACGGG GGCTTCCTGC TGGCCTCCTA TGACGAGGAG 
AGCGAGGAGT TCCAGGCCAT CTGCAAGCTC GGAACTGGCT TCAGTGACGA GGAGCTGGAA 
GAGCACCACC AGAGGCTCCA GGCCTTAGTG CTGCCCACCC CGCGCTCCTA CGTTCGGGCT 
GACGGTGCGG TGGCCCCCGA CCACTGGCTG GACCCCAGCG ACGTGTGGGA GGTGAAGTGC 
GCGGACCTCT CCCTGTCCCC CATCTACCCT GCGGCCCGAG GCCTGGTGGA CAGCGAGAAG 
GGGATCTCCC TCCGCTTCCC TCGGTTTATT CGCGTCCGTG AGGATAAGAA GCCGGAGCAG 
GCCACTACCA GTGCCCAGGT GGCCGGTCTG TACAAGAAGC AGAGTCAGAT TCAGAACCAG 
CAGGGTGCGG AGCCGGACTC CGAGCAGGAA GAGTTCTACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010813284.1
CDS	477..3398
Translation	MERLGPQPEGLLVTSPALLCGCRNRKLVTSGEEEWGENSDTIMQRSIMSFFQPKKEGKAKKPEKETAKSIRETESPPKVALKERNRVVSEGDSPVKRPGRKATRVLGSEGEEEEEEAPTTPRSQKPASDSPQASPPSPVTLPESSPSHSKPSPAVASPSGIPKRRTARKQLPKRTFQDVLKEQGEDKDPETKKKKEEEAETPIESLPEPEGDDKEEVEGGDQPTTPPEPRKTSTESLTESVSEPGVTVKQEPQEDGQAQPPPKTPKTLSSFFCEWGLHLAGLRREEAAPRKPAVKKEAKEEGPGALRKDEIKGHVDPASYNPAKNNYHPIEDACWKAGQRVPYLAVARAFEKIEEVSARLRMVETLSNLLRSVVALSPADLLPVLYLSLNRLGPPQQGLELGIGEGILLKAVAQATGRQLESVRAEVAEKGDVGLVAESSRSTQRLVLPPPALTAAGVFAKFRDIAQLAGSASTTKKIDVIKGLFVACRHSEARFIARALSGRLRLGLAEQSVLAALAQAVSLTPPSQECPPAVVDAGKGKTAEARKTWLEEQGMILKQTFCEVPDLERIVPVLLEHGLERLPEHCRLSPGVPLKPMLAHPTRGVSEVLKRFEEAAFTCEYKYDGQRAQIHVLEGGEVKIFSRNQEDNTGRYPDIISRIPKIKLPSVTSFILDTEAVAWDREKKQIQPFQVLTTRKRKEVDAAEIQVQVCLYAFDLIYLNGKSLVREPLSRRRQLLRENFVETEGEFVFATSLDTKDTEQIAEFLEQSVKDSCEGLMVKTLDVDATYEIAKRSHNWLKLKKDYLEGVGDTLDLVVIGAYLGKGKRAGRYGGFLLASYDEESEEFQAICKLGTGFSDEELEEHHQRLQALVLPTPRSYVRADGAVAPDHWLDPSDVWEVKCADLSLSPIYPAARGLVDSEKGISLRFPRFIRVREDKKPEQATTSAQVAGLYKKQSQIQNQQGAEPDSEQEEFY

Target ORF information:

RefSeq Version	XM_010814982.2
Organism	Bos taurus(cattle)
Definition	Bos taurus ligase I, DNA, ATP-dependent (LIG1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010814982.2

ORF Insert Sequence:

ATGGAGAGGC TTGGACCGCA ACCCGAAGGA CTCCTGGTGA CCAGTCCCGC TCTACTCTGC 
GGTTGCAGGA ACAGGAAACT GGTGACCAGC GGAGAGGAGG AGTGGGGAGA GAATTCTGAT 
ACCATCATGC AGCGAAGTAT CATGTCATTT TTCCAGCCCA AGAAAGAGGG CAAAGCGAAG 
AAGCCAGAGA AGGAGACAGC CAAGAGCATC AGAGAAACAG AGTCCCCTCC GAAGGTGGCG 
CTGAAGGAGC GGAATCGAGT GGTGTCTGAG GGTGACTCTC CAGTGAAGAG GCCAGGGAGG 
AAGGCGACCC GGGTCCTGGG CAGCGAGGGC GAGGAGGAGG AAGAGGAAGC CCCCACCACC 
CCCAGAAGCC AGAAGCCTGC TTCAGACTCT CCACAGGCCT CGCCTCCCAG TCCTGTCACA 
CTGCCTGAGA GCAGCCCTTC CCACTCCAAA CCCTCCCCAG CAGTTGCATC CCCATCAGGG 
ATCCCGAAGC GTCGCACGGC CCGGAAGCAG CTTCCCAAAC GGACATTTCA AGATGTTCTG 
AAAGAGCAGG GCGAGGACAA GGATCCAGAA ACTAAGAAGA AGAAGGAGGA AGAAGCGGAG 
ACACCAATAG AAAGCCTCCC AGAGCCTGAA GGGGACGACA AGGAGGAAGT GGAAGGTGGG 
GACCAGCCCA CGACGCCCCC TGAGCCCCGG AAGACCTCCA CAGAGTCCCT GACAGAAAGC 
GTCTCAGAGC CCGGGGTGAC GGTGAAGCAG GAACCACAGG AGGATGGCCA GGCTCAACCG 
CCCCCCAAAA CTCCAAAGAC CCTCAGCAGT TTCTTCTGTG AGTGGGGACT TCACCTTGCG 
GGGCTGCGCA GGGAAGAGGC AGCCCCCCGG AAGCCAGCTG TCAAAAAAGA AGCGAAAGAA 
GAAGGACCTG GTGCTCTGAG AAAGGACGAG ATCAAGGGAC ACGTGGACCC AGCGAGCTAT 
AATCCTGCCA AGAACAACTA TCACCCCATC GAAGATGCCT GCTGGAAGGC GGGCCAGAGG 
GTTCCTTATC TGGCTGTGGC CCGGGCGTTC GAGAAGATCG AGGAGGTCTC TGCTCGGCTC 
CGGATGGTGG AGACGCTGAG CAACCTGCTG CGCTCCGTGG TGGCCCTGTC ACCTGCTGAC 
CTCCTGCCCG TCCTCTACCT GAGCCTCAAC CGCCTGGGGC CGCCCCAGCA GGGCCTGGAG 
CTCGGCATCG GAGAGGGCAT CCTCCTCAAG GCAGTGGCCC AGGCCACGGG TCGGCAGCTG 
GAGTCCGTCC GGGCCGAGGT GGCCGAGAAG GGTGATGTGG GGCTGGTGGC CGAGAGCAGC 
CGCAGCACCC AGAGGCTCGT GCTGCCCCCG CCTGCGCTCA CTGCCGCCGG CGTCTTCGCC 
AAGTTCCGGG ACATCGCGCA GCTGGCAGGC AGCGCTTCCA CAACCAAGAA GATTGACGTC 
ATCAAAGGCC TCTTTGTGGC CTGCCGTCAC TCAGAGGCAC GTTTCATTGC CAGAGCCCTG 
AGTGGACGGC TGCGCCTCGG GTTGGCTGAG CAGTCGGTGC TGGCGGCCCT CGCCCAGGCC 
GTGAGCCTCA CGCCCCCCAG TCAAGAATGC CCCCCCGCCG TGGTGGACGC CGGGAAGGGC 
AAGACGGCAG AAGCCAGAAA GACGTGGCTG GAGGAGCAAG GCATGATCCT GAAGCAGACT 
TTCTGTGAGG TACCTGACCT GGAGCGGATC GTCCCCGTGC TTCTGGAACA CGGCCTGGAG 
CGGCTCCCGG AGCACTGCAG ACTGAGCCCA GGGGTTCCCC TGAAACCAAT GTTGGCCCAC 
CCCACCCGGG GTGTCAGTGA GGTCCTGAAG CGCTTTGAGG AGGCAGCTTT CACCTGCGAG 
TACAAATACG ACGGGCAGAG GGCACAGATC CACGTTCTGG AAGGCGGGGA GGTAAAGATC 
TTCAGCAGGA ATCAGGAGGA CAATACTGGA AGGTACCCCG ACATTATCAG CCGCATCCCT 
AAGATTAAAC TCCCATCGGT CACATCCTTC ATCCTAGACA CAGAAGCTGT AGCTTGGGAC 
CGGGAAAAGA AGCAGATCCA GCCATTCCAA GTCCTCACCA CCCGCAAACG CAAGGAGGTG 
GACGCGGCGG AGATCCAGGT GCAGGTGTGT CTGTATGCCT TCGATCTCAT TTACCTCAAC 
GGAAAGTCCC TGGTACGTGA GCCCCTCTCC CGACGCCGGC AGCTGCTCCG GGAGAACTTC 
GTGGAGACGG AGGGCGAGTT CGTCTTTGCC ACCTCCCTAG ACACCAAGGA CACGGAGCAG 
ATCGCAGAGT TCTTGGAGCA GTCGGTGAAA GACTCCTGCG AGGGGCTGAT GGTGAAGACC 
CTGGACGTGG ACGCCACCTA CGAGATCGCC AAGAGGTCAC ACAACTGGCT CAAGCTGAAG 
AAGGACTACC TCGAGGGTGT GGGCGACACC CTGGACCTCG TAGTGATTGG TGCCTACCTG 
GGCAAGGGGA AACGGGCCGG CCGGTACGGG GGCTTCCTGC TGGCCTCCTA TGACGAGGAG 
AGCGAGGAGT TCCAGGCCAT CTGCAAGCTC GGAACTGGCT TCAGTGACGA GGAGCTGGAA 
GAGCACCACC AGAGGCTCCA GGCCTTAGTG CTGCCCACCC CGCGCTCCTA CGTTCGGGCT 
GACGGTGCGG TGGCCCCCGA CCACTGGCTG GACCCCAGCG ACGTGTGGGA GGTGAAGTGC 
GCGGACCTCT CCCTGTCCCC CATCTACCCT GCGGCCCGAG GCCTGGTGGA CAGCGAGAAG 
GGGATCTCCC TCCGCTTCCC TCGGTTTATT CGCGTCCGTG AGGATAAGAA GCCGGAGCAG 
GCCACTACCA GTGCCCAGGT GGCCGGTCTG TACAAGAAGC AGAGTCAGAT TCAGAACCAG 
CAGGGTGCGG AGCCGGACTC CGAGCAGGAA GAGTTCTACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIG1 ( XM_010814980.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010813282.2 Bos taurus DNA ligase 1 (LIG1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa224759
Clone ID Related Accession (Same CDS sequence)	XM_010814980.3
Accession Version	XM_010814980.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2796bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	DNA ligase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010814980.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCGAA GTATCATGTC ATTTTTCCAG CCCAAGAAAG AGGGCAAAGC GAAGAAGCCA 
GAGAAGGAGA CAGCCAAGAG CATCAGAGAA ACAGAGTCCC CTCCGAAGGT GGCGCTGAAG 
GAGCGGAATC GAGTGGTGTC TGAGGGTGAC TCTCCAGTGA AGAGGCCAGG GAGGAAGGCG 
ACCCAGGTCC TGGGCAGCGA GGGCGAGGAG GAGGAAGAGG AAGCCCCCAC CACCCCCAGA 
AGCCAGAAGC CTGCTTCAGA CTCTCCACAG GCCTCGCCTC CCAGTCCTGT CACACTGCCT 
GAGAGCAGCC CTTCCCACTC CAAACCCTCC CCAGCAGTTG CATCCCCATC AGGGATCCCG 
AAGCGTCGCA CGGCCCGGAA GCAGCTTCCC AAACGGACAT TTCAAGATGT TCTGAAAGAG 
CAGGGCGAGG ACAAGGATCC AGAAACTAAG AAGAAGAAGG AGGAAGAAGC GGAGACACCA 
ATAGAAAGCC TCCCAGAGCC TGAAGGGGAC GACAAGGAGG AAGTGGAAGG TGGGGACCAG 
CCCACGACGC CCCCTGAGCC CCGGAAGACC TCCACAGAGT CCCTGACAGA AAGCGTCTCA 
GAGCCCGGGG TGACGGTGAA GCAGGAACCA CAGGAGGATG GCCAGGCTCA ACCGCCCCCC 
AAAACTCCAA AGACCCTCAG CAGTTTCTTC TGTGAGTGGG GACTTCACCT TGCGGGGCTG 
CGCAGGGAAG AGGCAGCCCC CCGGAAGCCA GCTGTCAAAA AAGAAGCGAA AGAAGAAGGA 
CCTGGTGCTC TGAGAAAGGA CGAGATCAAG GGACACGTGG ACCCAGCGAG CTATAATCCT 
GCCAAGAACA ACTATCACCC CATCGAAGAT GCCTGCTGGA AGGCGGGCCA GAGGGTTCCT 
TATCTGGCTG TGGCCCGGGC GTTCGAGAAG ATCGAGGAGG TCTCTGCTCG GCTCCGGATG 
GTGGAGACGC TGAGCAACCT GCTGCGCTCC GTGGTGGCCC TGTCACCTGC TGACCTCCTG 
CCCGTCCTCT ACCTGAGCCT CAACCGCCTG GGGCCGCCCC AGCAGGGCCT GGAGCTCGGC 
ATCGGAGAGG GCATCCTCCT CAAGGCAGTG GCCCAGGCCA CGGGTCGGCA GCTGGAGTCC 
GTCCGGGCCG AGGTGGCCGA GAAGGGTGAT GTGGGGCTGG TGGCCGAGAG CAGCCGCAGC 
ACCCAGAGGC TCGTGCTGCC CCCGCCTGCG CTCACTGCCG CCGGCGTCTT CGCCAAGTTC 
CGGGACATCG CGCAGCTGGC AGGCAGCGCT TCCACAACCA AGAAGATTGA CGTCATCAAA 
GGCCTCTTTG TGGCCTGCCG TCACTCAGAG GCACGTTTCA TTGCCAGAGC CCTGAGTGGA 
CGGCTGCGCC TCGGGTTGGC TGAGCAGTCG GTGCTGGCGG CCCTCGCCCA GGCCGTGAGC 
CTCACGCCCC CCAGTCAAGA ATGCCCCCCC GCCGTGGTGG ACGCCGGGAA GGGCAAGACG 
GCAGAAGCCA GAAAGACGTG GCTGGAGGAG CAAGGCATGA TCCTGAAGCA GACTTTCTGT 
GAGGTACCTG ACCTGGAGCG GATCGTCCCC GTGCTTCTGG AACACGGCCT GGAGCGGCTC 
CCGGAGCACT GCAGACTGAG CCCAGGGGTT CCCCTGAAAC CAATGTTGGC CCACCCCACC 
CGGGGTGTCA GTGAGGTCCT GAAGCGCTTT GAGGAGGCAG CTTTCACCTG CGAGTACAAA 
TACGACGGGC AGAGGGCACA GATCCACGTT CTGGAAGGCG GGGAGGTAAA GATCTTCAGC 
AGGAATCAGG AGGACAATAC TGGAAGGTAC CCCGACATTA TCAGCCGCAT CCCTAAGATT 
AAACTCCCAT CGGTCACATC CTTCATCCTA GACACAGAAG CTGTAGCTTG GGACCGGGAA 
AAGAAGCAGA TCCAGCCATT CCAAGTCCTC ACCACCCGCA AACGCAAGGA GGTGGACGCG 
GCGGAGATCC AGGTGCAGGT GTGTCTGTAT GCCTTCGATC TCATCTACCT CAACGGAGAG 
TCCCTGGTAC GTGAGCCCCT CTCCCGACGC CGGCAGCTGC TCCGGGAGAA CTTCGTGGAG 
ACGGAGGGCG AGTTCGTCTT TGCCACCTCC CTAGACACCA AGGACACGGA GCAGATCGCA 
GAGTTCTTGG AGCAGTCGGT GAAAGACTCC TGCGAGGGGC TGATGGTGAA GACCCTGGAT 
GTGGACGCCA CCTACGAGAT CGCCAAGAGG TCACACAACT GGCTCAAGCT GAAGAAGGAC 
TACCTCGAGG GTGTGGGCGA CACCCTGGAC CTCGTAGTGA TTGGTGCCTA CCTGGGCAAG 
GGGAAACGGG CCGGCCGGTA CGGGGGCTTC CTGCTGGCCT CCTATGACGA GGAGAGCGAG 
GAGTTCCAGG CCATCTGCAA GCTCGGAACT GGCTTCAGTG ACGAGGAGCT GGAAGAGCAC 
CACCAGAGGC TCCAGGCCTT AGTGCTGCCC ACCCCGCGCT CCTACGTTCG GGCTGACGGT 
GCGGTGGCCC CCGACCACTG GCTGGACCCC AGCGACGTGT GGGAGGTGAA GTGCGCGGAC 
CTCTCCCTGT CCCCCATCTA CCCTGCGGCC CGAGGCCTGG TGGACAGCGA GAAGGGGATC 
TCCCTCCGCT TCCCTCGGTT TATTCGCGTC CGTGAGGATA AGAAGCCGGA GCAGGCCACT 
ACCAGTGCCC AGGTGGCCGG TCTGTACAAG AAGCAGAGTC AGATTCAGAA CCAGCAGGGT 
GCGGAGCCGG ACTCCGAGCA GGAAGAGTTC TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010813282.2
CDS	191..2986
Translation	MQRSIMSFFQPKKEGKAKKPEKETAKSIRETESPPKVALKERNRVVSEGDSPVKRPGRKATQVLGSEGEEEEEEAPTTPRSQKPASDSPQASPPSPVTLPESSPSHSKPSPAVASPSGIPKRRTARKQLPKRTFQDVLKEQGEDKDPETKKKKEEEAETPIESLPEPEGDDKEEVEGGDQPTTPPEPRKTSTESLTESVSEPGVTVKQEPQEDGQAQPPPKTPKTLSSFFCEWGLHLAGLRREEAAPRKPAVKKEAKEEGPGALRKDEIKGHVDPASYNPAKNNYHPIEDACWKAGQRVPYLAVARAFEKIEEVSARLRMVETLSNLLRSVVALSPADLLPVLYLSLNRLGPPQQGLELGIGEGILLKAVAQATGRQLESVRAEVAEKGDVGLVAESSRSTQRLVLPPPALTAAGVFAKFRDIAQLAGSASTTKKIDVIKGLFVACRHSEARFIARALSGRLRLGLAEQSVLAALAQAVSLTPPSQECPPAVVDAGKGKTAEARKTWLEEQGMILKQTFCEVPDLERIVPVLLEHGLERLPEHCRLSPGVPLKPMLAHPTRGVSEVLKRFEEAAFTCEYKYDGQRAQIHVLEGGEVKIFSRNQEDNTGRYPDIISRIPKIKLPSVTSFILDTEAVAWDREKKQIQPFQVLTTRKRKEVDAAEIQVQVCLYAFDLIYLNGESLVREPLSRRRQLLRENFVETEGEFVFATSLDTKDTEQIAEFLEQSVKDSCEGLMVKTLDVDATYEIAKRSHNWLKLKKDYLEGVGDTLDLVVIGAYLGKGKRAGRYGGFLLASYDEESEEFQAICKLGTGFSDEELEEHHQRLQALVLPTPRSYVRADGAVAPDHWLDPSDVWEVKCADLSLSPIYPAARGLVDSEKGISLRFPRFIRVREDKKPEQATTSAQVAGLYKKQSQIQNQQGAEPDSEQEEFY

Target ORF information:

RefSeq Version	XM_010814980.3
Organism	Bos taurus(cattle)
Definition	Bos taurus DNA ligase 1 (LIG1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010814980.3

ORF Insert Sequence:

ATGCAGCGAA GTATCATGTC ATTTTTCCAG CCCAAGAAAG AGGGCAAAGC GAAGAAGCCA 
GAGAAGGAGA CAGCCAAGAG CATCAGAGAA ACAGAGTCCC CTCCGAAGGT GGCGCTGAAG 
GAGCGGAATC GAGTGGTGTC TGAGGGTGAC TCTCCAGTGA AGAGGCCAGG GAGGAAGGCG 
ACCCAGGTCC TGGGCAGCGA GGGCGAGGAG GAGGAAGAGG AAGCCCCCAC CACCCCCAGA 
AGCCAGAAGC CTGCTTCAGA CTCTCCACAG GCCTCGCCTC CCAGTCCTGT CACACTGCCT 
GAGAGCAGCC CTTCCCACTC CAAACCCTCC CCAGCAGTTG CATCCCCATC AGGGATCCCG 
AAGCGTCGCA CGGCCCGGAA GCAGCTTCCC AAACGGACAT TTCAAGATGT TCTGAAAGAG 
CAGGGCGAGG ACAAGGATCC AGAAACTAAG AAGAAGAAGG AGGAAGAAGC GGAGACACCA 
ATAGAAAGCC TCCCAGAGCC TGAAGGGGAC GACAAGGAGG AAGTGGAAGG TGGGGACCAG 
CCCACGACGC CCCCTGAGCC CCGGAAGACC TCCACAGAGT CCCTGACAGA AAGCGTCTCA 
GAGCCCGGGG TGACGGTGAA GCAGGAACCA CAGGAGGATG GCCAGGCTCA ACCGCCCCCC 
AAAACTCCAA AGACCCTCAG CAGTTTCTTC TGTGAGTGGG GACTTCACCT TGCGGGGCTG 
CGCAGGGAAG AGGCAGCCCC CCGGAAGCCA GCTGTCAAAA AAGAAGCGAA AGAAGAAGGA 
CCTGGTGCTC TGAGAAAGGA CGAGATCAAG GGACACGTGG ACCCAGCGAG CTATAATCCT 
GCCAAGAACA ACTATCACCC CATCGAAGAT GCCTGCTGGA AGGCGGGCCA GAGGGTTCCT 
TATCTGGCTG TGGCCCGGGC GTTCGAGAAG ATCGAGGAGG TCTCTGCTCG GCTCCGGATG 
GTGGAGACGC TGAGCAACCT GCTGCGCTCC GTGGTGGCCC TGTCACCTGC TGACCTCCTG 
CCCGTCCTCT ACCTGAGCCT CAACCGCCTG GGGCCGCCCC AGCAGGGCCT GGAGCTCGGC 
ATCGGAGAGG GCATCCTCCT CAAGGCAGTG GCCCAGGCCA CGGGTCGGCA GCTGGAGTCC 
GTCCGGGCCG AGGTGGCCGA GAAGGGTGAT GTGGGGCTGG TGGCCGAGAG CAGCCGCAGC 
ACCCAGAGGC TCGTGCTGCC CCCGCCTGCG CTCACTGCCG CCGGCGTCTT CGCCAAGTTC 
CGGGACATCG CGCAGCTGGC AGGCAGCGCT TCCACAACCA AGAAGATTGA CGTCATCAAA 
GGCCTCTTTG TGGCCTGCCG TCACTCAGAG GCACGTTTCA TTGCCAGAGC CCTGAGTGGA 
CGGCTGCGCC TCGGGTTGGC TGAGCAGTCG GTGCTGGCGG CCCTCGCCCA GGCCGTGAGC 
CTCACGCCCC CCAGTCAAGA ATGCCCCCCC GCCGTGGTGG ACGCCGGGAA GGGCAAGACG 
GCAGAAGCCA GAAAGACGTG GCTGGAGGAG CAAGGCATGA TCCTGAAGCA GACTTTCTGT 
GAGGTACCTG ACCTGGAGCG GATCGTCCCC GTGCTTCTGG AACACGGCCT GGAGCGGCTC 
CCGGAGCACT GCAGACTGAG CCCAGGGGTT CCCCTGAAAC CAATGTTGGC CCACCCCACC 
CGGGGTGTCA GTGAGGTCCT GAAGCGCTTT GAGGAGGCAG CTTTCACCTG CGAGTACAAA 
TACGACGGGC AGAGGGCACA GATCCACGTT CTGGAAGGCG GGGAGGTAAA GATCTTCAGC 
AGGAATCAGG AGGACAATAC TGGAAGGTAC CCCGACATTA TCAGCCGCAT CCCTAAGATT 
AAACTCCCAT CGGTCACATC CTTCATCCTA GACACAGAAG CTGTAGCTTG GGACCGGGAA 
AAGAAGCAGA TCCAGCCATT CCAAGTCCTC ACCACCCGCA AACGCAAGGA GGTGGACGCG 
GCGGAGATCC AGGTGCAGGT GTGTCTGTAT GCCTTCGATC TCATCTACCT CAACGGAGAG 
TCCCTGGTAC GTGAGCCCCT CTCCCGACGC CGGCAGCTGC TCCGGGAGAA CTTCGTGGAG 
ACGGAGGGCG AGTTCGTCTT TGCCACCTCC CTAGACACCA AGGACACGGA GCAGATCGCA 
GAGTTCTTGG AGCAGTCGGT GAAAGACTCC TGCGAGGGGC TGATGGTGAA GACCCTGGAT 
GTGGACGCCA CCTACGAGAT CGCCAAGAGG TCACACAACT GGCTCAAGCT GAAGAAGGAC 
TACCTCGAGG GTGTGGGCGA CACCCTGGAC CTCGTAGTGA TTGGTGCCTA CCTGGGCAAG 
GGGAAACGGG CCGGCCGGTA CGGGGGCTTC CTGCTGGCCT CCTATGACGA GGAGAGCGAG 
GAGTTCCAGG CCATCTGCAA GCTCGGAACT GGCTTCAGTG ACGAGGAGCT GGAAGAGCAC 
CACCAGAGGC TCCAGGCCTT AGTGCTGCCC ACCCCGCGCT CCTACGTTCG GGCTGACGGT 
GCGGTGGCCC CCGACCACTG GCTGGACCCC AGCGACGTGT GGGAGGTGAA GTGCGCGGAC 
CTCTCCCTGT CCCCCATCTA CCCTGCGGCC CGAGGCCTGG TGGACAGCGA GAAGGGGATC 
TCCCTCCGCT TCCCTCGGTT TATTCGCGTC CGTGAGGATA AGAAGCCGGA GCAGGCCACT 
ACCAGTGCCC AGGTGGCCGG TCTGTACAAG AAGCAGAGTC AGATTCAGAA CCAGCAGGGT 
GCGGAGCCGG ACTCCGAGCA GGAAGAGTTC TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIG1 ( XM_010814981.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010813283.2 Bos taurus DNA ligase 1 (LIG1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa224760
Clone ID Related Accession (Same CDS sequence)	XM_010814981.3
Accession Version	XM_010814981.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2793bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	DNA ligase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010814981.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCGAA GTATCATGTC ATTTTTCCAG CCCAAGAAAG AGGGCAAAGC GAAGAAGCCA 
GAGAAGGAGA CAGCCAAGAG CATCAGAGAA ACAGAGTCCC CTCCGAAGGT GGCGCTGAAG 
GAGCGGAATC GAGTGGTGTC TGAGGGTGAC TCTCCAGTGA AGAGGCCAGG GAGGAAGGCG 
ACCCAGGTCC TGGGCAGCGA GGGCGAGGAG GAGGAAGAGG AAGCCCCCAC CACCCCCAGA 
AGCCAGAAGC CTGCTTCAGA CTCTCCACAG GCCTCGCCTC CCAGTCCTGT CACACTGCCT 
GAGAGCAGCC CTTCCCACTC CAAACCCTCC CCAGCAGTTG CATCCCCATC AGGGATCCCG 
AAGCGTCGCA CGGCCCGGAA GCAGCTTCCC AAACGGACAT TTCAAGATGT TCTGAAAGAG 
CAGGGCGAGG ACAAGGATCC AGAAACTAAG AAGAAGAAGG AGGAAGAAGC GGAGACACCA 
ATAGAAAGCC TCCCAGAGCC TGAAGGGGAC GACAAGGAGG AAGTGGAAGG TGGGGACCAG 
CCCACGACGC CCCCTGAGCC CCGGAAGACC TCCAAGTCCC TGACAGAAAG CGTCTCAGAG 
CCCGGGGTGA CGGTGAAGCA GGAACCACAG GAGGATGGCC AGGCTCAACC GCCCCCCAAA 
ACTCCAAAGA CCCTCAGCAG TTTCTTCTGT GAGTGGGGAC TTCACCTTGC GGGGCTGCGC 
AGGGAAGAGG CAGCCCCCCG GAAGCCAGCT GTCAAAAAAG AAGCGAAAGA AGAAGGACCT 
GGTGCTCTGA GAAAGGACGA GATCAAGGGA CACGTGGACC CAGCGAGCTA TAATCCTGCC 
AAGAACAACT ATCACCCCAT CGAAGATGCC TGCTGGAAGG CGGGCCAGAG GGTTCCTTAT 
CTGGCTGTGG CCCGGGCGTT CGAGAAGATC GAGGAGGTCT CTGCTCGGCT CCGGATGGTG 
GAGACGCTGA GCAACCTGCT GCGCTCCGTG GTGGCCCTGT CACCTGCTGA CCTCCTGCCC 
GTCCTCTACC TGAGCCTCAA CCGCCTGGGG CCGCCCCAGC AGGGCCTGGA GCTCGGCATC 
GGAGAGGGCA TCCTCCTCAA GGCAGTGGCC CAGGCCACGG GTCGGCAGCT GGAGTCCGTC 
CGGGCCGAGG TGGCCGAGAA GGGTGATGTG GGGCTGGTGG CCGAGAGCAG CCGCAGCACC 
CAGAGGCTCG TGCTGCCCCC GCCTGCGCTC ACTGCCGCCG GCGTCTTCGC CAAGTTCCGG 
GACATCGCGC AGCTGGCAGG CAGCGCTTCC ACAACCAAGA AGATTGACGT CATCAAAGGC 
CTCTTTGTGG CCTGCCGTCA CTCAGAGGCA CGTTTCATTG CCAGAGCCCT GAGTGGACGG 
CTGCGCCTCG GGTTGGCTGA GCAGTCGGTG CTGGCGGCCC TCGCCCAGGC CGTGAGCCTC 
ACGCCCCCCA GTCAAGAATG CCCCCCCGCC GTGGTGGACG CCGGGAAGGG CAAGACGGCA 
GAAGCCAGAA AGACGTGGCT GGAGGAGCAA GGCATGATCC TGAAGCAGAC TTTCTGTGAG 
GTACCTGACC TGGAGCGGAT CGTCCCCGTG CTTCTGGAAC ACGGCCTGGA GCGGCTCCCG 
GAGCACTGCA GACTGAGCCC AGGGGTTCCC CTGAAACCAA TGTTGGCCCA CCCCACCCGG 
GGTGTCAGTG AGGTCCTGAA GCGCTTTGAG GAGGCAGCTT TCACCTGCGA GTACAAATAC 
GACGGGCAGA GGGCACAGAT CCACGTTCTG GAAGGCGGGG AGGTAAAGAT CTTCAGCAGG 
AATCAGGAGG ACAATACTGG AAGGTACCCC GACATTATCA GCCGCATCCC TAAGATTAAA 
CTCCCATCGG TCACATCCTT CATCCTAGAC ACAGAAGCTG TAGCTTGGGA CCGGGAAAAG 
AAGCAGATCC AGCCATTCCA AGTCCTCACC ACCCGCAAAC GCAAGGAGGT GGACGCGGCG 
GAGATCCAGG TGCAGGTGTG TCTGTATGCC TTCGATCTCA TCTACCTCAA CGGAGAGTCC 
CTGGTACGTG AGCCCCTCTC CCGACGCCGG CAGCTGCTCC GGGAGAACTT CGTGGAGACG 
GAGGGCGAGT TCGTCTTTGC CACCTCCCTA GACACCAAGG ACACGGAGCA GATCGCAGAG 
TTCTTGGAGC AGTCGGTGAA AGACTCCTGC GAGGGGCTGA TGGTGAAGAC CCTGGATGTG 
GACGCCACCT ACGAGATCGC CAAGAGGTCA CACAACTGGC TCAAGCTGAA GAAGGACTAC 
CTCGAGGGTG TGGGCGACAC CCTGGACCTC GTAGTGATTG GTGCCTACCT GGGCAAGGGG 
AAACGGGCCG GCCGGTACGG GGGCTTCCTG CTGGCCTCCT ATGACGAGGA GAGCGAGGAG 
TTCCAGGCCA TCTGCAAGCT CGGAACTGGC TTCAGTGACG AGGAGCTGGA AGAGCACCAC 
CAGAGGCTCC AGGCCTTAGT GCTGCCCACC CCGCGCTCCT ACGTTCGGGC TGACGGTGCG 
GTGGCCCCCG ACCACTGGCT GGACCCCAGC GACGTGTGGG AGGTGAAGTG CGCGGACCTC 
TCCCTGTCCC CCATCTACCC TGCGGCCCGA GGCCTGGTGG ACAGCGAGAA GGGGATCTCC 
CTCCGCTTCC CTCGGTTTAT TCGCGTCCGT GAGGATAAGA AGCCGGAGCA GGCCACTACC 
AGTGCCCAGG TGGCCGGTCT GTACAAGAAG CAGAGTCAGA TTCAGAACCA GCAGGGTGCG 
GAGCCGGACT CCGAGCAGGA AGAGTTCTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010813283.2
CDS	191..2983
Translation	MQRSIMSFFQPKKEGKAKKPEKETAKSIRETESPPKVALKERNRVVSEGDSPVKRPGRKATQVLGSEGEEEEEEAPTTPRSQKPASDSPQASPPSPVTLPESSPSHSKPSPAVASPSGIPKRRTARKQLPKRTFQDVLKEQGEDKDPETKKKKEEEAETPIESLPEPEGDDKEEVEGGDQPTTPPEPRKTSKSLTESVSEPGVTVKQEPQEDGQAQPPPKTPKTLSSFFCEWGLHLAGLRREEAAPRKPAVKKEAKEEGPGALRKDEIKGHVDPASYNPAKNNYHPIEDACWKAGQRVPYLAVARAFEKIEEVSARLRMVETLSNLLRSVVALSPADLLPVLYLSLNRLGPPQQGLELGIGEGILLKAVAQATGRQLESVRAEVAEKGDVGLVAESSRSTQRLVLPPPALTAAGVFAKFRDIAQLAGSASTTKKIDVIKGLFVACRHSEARFIARALSGRLRLGLAEQSVLAALAQAVSLTPPSQECPPAVVDAGKGKTAEARKTWLEEQGMILKQTFCEVPDLERIVPVLLEHGLERLPEHCRLSPGVPLKPMLAHPTRGVSEVLKRFEEAAFTCEYKYDGQRAQIHVLEGGEVKIFSRNQEDNTGRYPDIISRIPKIKLPSVTSFILDTEAVAWDREKKQIQPFQVLTTRKRKEVDAAEIQVQVCLYAFDLIYLNGESLVREPLSRRRQLLRENFVETEGEFVFATSLDTKDTEQIAEFLEQSVKDSCEGLMVKTLDVDATYEIAKRSHNWLKLKKDYLEGVGDTLDLVVIGAYLGKGKRAGRYGGFLLASYDEESEEFQAICKLGTGFSDEELEEHHQRLQALVLPTPRSYVRADGAVAPDHWLDPSDVWEVKCADLSLSPIYPAARGLVDSEKGISLRFPRFIRVREDKKPEQATTSAQVAGLYKKQSQIQNQQGAEPDSEQEEFY

Target ORF information:

RefSeq Version	XM_010814981.3
Organism	Bos taurus(cattle)
Definition	Bos taurus DNA ligase 1 (LIG1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010814981.3

ORF Insert Sequence:

ATGCAGCGAA GTATCATGTC ATTTTTCCAG CCCAAGAAAG AGGGCAAAGC GAAGAAGCCA 
GAGAAGGAGA CAGCCAAGAG CATCAGAGAA ACAGAGTCCC CTCCGAAGGT GGCGCTGAAG 
GAGCGGAATC GAGTGGTGTC TGAGGGTGAC TCTCCAGTGA AGAGGCCAGG GAGGAAGGCG 
ACCCAGGTCC TGGGCAGCGA GGGCGAGGAG GAGGAAGAGG AAGCCCCCAC CACCCCCAGA 
AGCCAGAAGC CTGCTTCAGA CTCTCCACAG GCCTCGCCTC CCAGTCCTGT CACACTGCCT 
GAGAGCAGCC CTTCCCACTC CAAACCCTCC CCAGCAGTTG CATCCCCATC AGGGATCCCG 
AAGCGTCGCA CGGCCCGGAA GCAGCTTCCC AAACGGACAT TTCAAGATGT TCTGAAAGAG 
CAGGGCGAGG ACAAGGATCC AGAAACTAAG AAGAAGAAGG AGGAAGAAGC GGAGACACCA 
ATAGAAAGCC TCCCAGAGCC TGAAGGGGAC GACAAGGAGG AAGTGGAAGG TGGGGACCAG 
CCCACGACGC CCCCTGAGCC CCGGAAGACC TCCAAGTCCC TGACAGAAAG CGTCTCAGAG 
CCCGGGGTGA CGGTGAAGCA GGAACCACAG GAGGATGGCC AGGCTCAACC GCCCCCCAAA 
ACTCCAAAGA CCCTCAGCAG TTTCTTCTGT GAGTGGGGAC TTCACCTTGC GGGGCTGCGC 
AGGGAAGAGG CAGCCCCCCG GAAGCCAGCT GTCAAAAAAG AAGCGAAAGA AGAAGGACCT 
GGTGCTCTGA GAAAGGACGA GATCAAGGGA CACGTGGACC CAGCGAGCTA TAATCCTGCC 
AAGAACAACT ATCACCCCAT CGAAGATGCC TGCTGGAAGG CGGGCCAGAG GGTTCCTTAT 
CTGGCTGTGG CCCGGGCGTT CGAGAAGATC GAGGAGGTCT CTGCTCGGCT CCGGATGGTG 
GAGACGCTGA GCAACCTGCT GCGCTCCGTG GTGGCCCTGT CACCTGCTGA CCTCCTGCCC 
GTCCTCTACC TGAGCCTCAA CCGCCTGGGG CCGCCCCAGC AGGGCCTGGA GCTCGGCATC 
GGAGAGGGCA TCCTCCTCAA GGCAGTGGCC CAGGCCACGG GTCGGCAGCT GGAGTCCGTC 
CGGGCCGAGG TGGCCGAGAA GGGTGATGTG GGGCTGGTGG CCGAGAGCAG CCGCAGCACC 
CAGAGGCTCG TGCTGCCCCC GCCTGCGCTC ACTGCCGCCG GCGTCTTCGC CAAGTTCCGG 
GACATCGCGC AGCTGGCAGG CAGCGCTTCC ACAACCAAGA AGATTGACGT CATCAAAGGC 
CTCTTTGTGG CCTGCCGTCA CTCAGAGGCA CGTTTCATTG CCAGAGCCCT GAGTGGACGG 
CTGCGCCTCG GGTTGGCTGA GCAGTCGGTG CTGGCGGCCC TCGCCCAGGC CGTGAGCCTC 
ACGCCCCCCA GTCAAGAATG CCCCCCCGCC GTGGTGGACG CCGGGAAGGG CAAGACGGCA 
GAAGCCAGAA AGACGTGGCT GGAGGAGCAA GGCATGATCC TGAAGCAGAC TTTCTGTGAG 
GTACCTGACC TGGAGCGGAT CGTCCCCGTG CTTCTGGAAC ACGGCCTGGA GCGGCTCCCG 
GAGCACTGCA GACTGAGCCC AGGGGTTCCC CTGAAACCAA TGTTGGCCCA CCCCACCCGG 
GGTGTCAGTG AGGTCCTGAA GCGCTTTGAG GAGGCAGCTT TCACCTGCGA GTACAAATAC 
GACGGGCAGA GGGCACAGAT CCACGTTCTG GAAGGCGGGG AGGTAAAGAT CTTCAGCAGG 
AATCAGGAGG ACAATACTGG AAGGTACCCC GACATTATCA GCCGCATCCC TAAGATTAAA 
CTCCCATCGG TCACATCCTT CATCCTAGAC ACAGAAGCTG TAGCTTGGGA CCGGGAAAAG 
AAGCAGATCC AGCCATTCCA AGTCCTCACC ACCCGCAAAC GCAAGGAGGT GGACGCGGCG 
GAGATCCAGG TGCAGGTGTG TCTGTATGCC TTCGATCTCA TCTACCTCAA CGGAGAGTCC 
CTGGTACGTG AGCCCCTCTC CCGACGCCGG CAGCTGCTCC GGGAGAACTT CGTGGAGACG 
GAGGGCGAGT TCGTCTTTGC CACCTCCCTA GACACCAAGG ACACGGAGCA GATCGCAGAG 
TTCTTGGAGC AGTCGGTGAA AGACTCCTGC GAGGGGCTGA TGGTGAAGAC CCTGGATGTG 
GACGCCACCT ACGAGATCGC CAAGAGGTCA CACAACTGGC TCAAGCTGAA GAAGGACTAC 
CTCGAGGGTG TGGGCGACAC CCTGGACCTC GTAGTGATTG GTGCCTACCT GGGCAAGGGG 
AAACGGGCCG GCCGGTACGG GGGCTTCCTG CTGGCCTCCT ATGACGAGGA GAGCGAGGAG 
TTCCAGGCCA TCTGCAAGCT CGGAACTGGC TTCAGTGACG AGGAGCTGGA AGAGCACCAC 
CAGAGGCTCC AGGCCTTAGT GCTGCCCACC CCGCGCTCCT ACGTTCGGGC TGACGGTGCG 
GTGGCCCCCG ACCACTGGCT GGACCCCAGC GACGTGTGGG AGGTGAAGTG CGCGGACCTC 
TCCCTGTCCC CCATCTACCC TGCGGCCCGA GGCCTGGTGG ACAGCGAGAA GGGGATCTCC 
CTCCGCTTCC CTCGGTTTAT TCGCGTCCGT GAGGATAAGA AGCCGGAGCA GGCCACTACC 
AGTGCCCAGG TGGCCGGTCT GTACAAGAAG CAGAGTCAGA TTCAGAACCA GCAGGGTGCG 
GAGCCGGACT CCGAGCAGGA AGAGTTCTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIG1 ( XM_005219131.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005219188.2 Bos taurus DNA ligase 1 (LIG1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa224761
Clone ID Related Accession (Same CDS sequence)	XM_005219131.4
Accession Version	XM_005219131.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2748bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	DNA ligase 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005219131.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCGAA GTATCATGTC ATTTTTCCAG CCCAAGAAAG AGGGCAAAGC GAAGAAGCCA 
GAGAAGGAGA CAGCCAAGAG CATCAGAGAA ACAGAGTCCC CTCCGAAGGT GGCGCTGAAG 
GAGCGGAATC GAGTGGTGTC TGAGGGTGAC TCTCCAGTGA AGAGGCCAGG GAGGAAGGCG 
ACCCAGGTCC TGGGCAGCGA GGGCGAGGAG GAGGAAGAGG AAGCCCCCAC CACCCCCAGA 
AGCCAGAAGC CTGCTTCAGA CTCTCCACAG GCCTCGCCTC CCAGTCCTGT CACACTGCCT 
GAGAGCAGCC CTTCCCACTC CAAACCCTCC CCAGCAGTTG CATCCCCATC AGGGATCCCG 
AAGCGTCGCA CGGCCCGGAA GCAGCTTCCC AAACGGACAT TTCAAGATGT TCTGAAAGAG 
CAGGGCGAGG ACAAGGATCC AGAAACTAAG AAGAAGAAGG AGGAAGAAGC GGAGACACCA 
ATAGAAAGCC TCCCAGAGCC TGAAGGGGAC GACAAGGAGG AAGTGGAAGG TGGGGACCAG 
CCCACGACGC CCCCTGAGCC CCGGAAGACC TCCAAGTCCC TGACAGAAAG CGTCTCAGAG 
CCCGGGGTGA CGGTGAAGCA GGAACCACAG GAGGATGGCC AGGCTCAACC GCCCCCCAAA 
ACTCCAAAGA CCCTCAGCAG TTTCTTCTCC CCCCGGAAGC CAGCTGTCAA AAAAGAAGCG 
AAAGAAGAAG GACCTGGTGC TCTGAGAAAG GACGAGATCA AGGGACACGT GGACCCAGCG 
AGCTATAATC CTGCCAAGAA CAACTATCAC CCCATCGAAG ATGCCTGCTG GAAGGCGGGC 
CAGAGGGTTC CTTATCTGGC TGTGGCCCGG GCGTTCGAGA AGATCGAGGA GGTCTCTGCT 
CGGCTCCGGA TGGTGGAGAC GCTGAGCAAC CTGCTGCGCT CCGTGGTGGC CCTGTCACCT 
GCTGACCTCC TGCCCGTCCT CTACCTGAGC CTCAACCGCC TGGGGCCGCC CCAGCAGGGC 
CTGGAGCTCG GCATCGGAGA GGGCATCCTC CTCAAGGCAG TGGCCCAGGC CACGGGTCGG 
CAGCTGGAGT CCGTCCGGGC CGAGGTGGCC GAGAAGGGTG ATGTGGGGCT GGTGGCCGAG 
AGCAGCCGCA GCACCCAGAG GCTCGTGCTG CCCCCGCCTG CGCTCACTGC CGCCGGCGTC 
TTCGCCAAGT TCCGGGACAT CGCGCAGCTG GCAGGCAGCG CTTCCACAAC CAAGAAGATT 
GACGTCATCA AAGGCCTCTT TGTGGCCTGC CGTCACTCAG AGGCACGTTT CATTGCCAGA 
GCCCTGAGTG GACGGCTGCG CCTCGGGTTG GCTGAGCAGT CGGTGCTGGC GGCCCTCGCC 
CAGGCCGTGA GCCTCACGCC CCCCAGTCAA GAATGCCCCC CCGCCGTGGT GGACGCCGGG 
AAGGGCAAGA CGGCAGAAGC CAGAAAGACG TGGCTGGAGG AGCAAGGCAT GATCCTGAAG 
CAGACTTTCT GTGAGGTACC TGACCTGGAG CGGATCGTCC CCGTGCTTCT GGAACACGGC 
CTGGAGCGGC TCCCGGAGCA CTGCAGACTG AGCCCAGGGG TTCCCCTGAA ACCAATGTTG 
GCCCACCCCA CCCGGGGTGT CAGTGAGGTC CTGAAGCGCT TTGAGGAGGC AGCTTTCACC 
TGCGAGTACA AATACGACGG GCAGAGGGCA CAGATCCACG TTCTGGAAGG CGGGGAGGTA 
AAGATCTTCA GCAGGAATCA GGAGGACAAT ACTGGAAGGT ACCCCGACAT TATCAGCCGC 
ATCCCTAAGA TTAAACTCCC ATCGGTCACA TCCTTCATCC TAGACACAGA AGCTGTAGCT 
TGGGACCGGG AAAAGAAGCA GATCCAGCCA TTCCAAGTCC TCACCACCCG CAAACGCAAG 
GAGGTGGACG CGGCGGAGAT CCAGGTGCAG GTGTGTCTGT ATGCCTTCGA TCTCATCTAC 
CTCAACGGAG AGTCCCTGGT ACGTGAGCCC CTCTCCCGAC GCCGGCAGCT GCTCCGGGAG 
AACTTCGTGG AGACGGAGGG CGAGTTCGTC TTTGCCACCT CCCTAGACAC CAAGGACACG 
GAGCAGATCG CAGAGTTCTT GGAGCAGTCG GTGAAAGACT CCTGCGAGGG GCTGATGGTG 
AAGACCCTGG ATGTGGACGC CACCTACGAG ATCGCCAAGA GGTCACACAA CTGGCTCAAG 
CTGAAGAAGG ACTACCTCGA GGGTGTGGGC GACACCCTGG ACCTCGTAGT GATTGGTGCC 
TACCTGGGCA AGGGGAAACG GGCCGGCCGG TACGGGGGCT TCCTGCTGGC CTCCTATGAC 
GAGGAGAGCG AGGAGTTCCA GGCCATCTGC AAGCTCGGAA CTGGCTTCAG TGACGAGGAG 
CTGGAAGAGC ACCACCAGAG GCTCCAGGCC TTAGTGCTGC CCACCCCGCG CTCCTACGTT 
CGGGCTGACG GTGCGGTGGC CCCCGACCAC TGGCTGGACC CCAGCGACGT GTGGGAGGTG 
AAGTGCGCGG ACCTCTCCCT GTCCCCCATC TACCCTGCGG CCCGAGGCCT GGTGGACAGC 
GAGAAGGGGA TCTCCCTCCG CTTCCCTCGG TTTATTCGCG TCCGTGAGGA TAAGAAGCCG 
GAGCAGGCCA CTACCAGTGC CCAGGTGGCC GGTCTGTACA AGAAGCAGAG TCAGATTCAG 
AACCAGCAGG GTGCGGAGCC GGACTCCGAG CAGGAAGAGT TCTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005219188.2
CDS	191..2938
Translation	MQRSIMSFFQPKKEGKAKKPEKETAKSIRETESPPKVALKERNRVVSEGDSPVKRPGRKATQVLGSEGEEEEEEAPTTPRSQKPASDSPQASPPSPVTLPESSPSHSKPSPAVASPSGIPKRRTARKQLPKRTFQDVLKEQGEDKDPETKKKKEEEAETPIESLPEPEGDDKEEVEGGDQPTTPPEPRKTSKSLTESVSEPGVTVKQEPQEDGQAQPPPKTPKTLSSFFSPRKPAVKKEAKEEGPGALRKDEIKGHVDPASYNPAKNNYHPIEDACWKAGQRVPYLAVARAFEKIEEVSARLRMVETLSNLLRSVVALSPADLLPVLYLSLNRLGPPQQGLELGIGEGILLKAVAQATGRQLESVRAEVAEKGDVGLVAESSRSTQRLVLPPPALTAAGVFAKFRDIAQLAGSASTTKKIDVIKGLFVACRHSEARFIARALSGRLRLGLAEQSVLAALAQAVSLTPPSQECPPAVVDAGKGKTAEARKTWLEEQGMILKQTFCEVPDLERIVPVLLEHGLERLPEHCRLSPGVPLKPMLAHPTRGVSEVLKRFEEAAFTCEYKYDGQRAQIHVLEGGEVKIFSRNQEDNTGRYPDIISRIPKIKLPSVTSFILDTEAVAWDREKKQIQPFQVLTTRKRKEVDAAEIQVQVCLYAFDLIYLNGESLVREPLSRRRQLLRENFVETEGEFVFATSLDTKDTEQIAEFLEQSVKDSCEGLMVKTLDVDATYEIAKRSHNWLKLKKDYLEGVGDTLDLVVIGAYLGKGKRAGRYGGFLLASYDEESEEFQAICKLGTGFSDEELEEHHQRLQALVLPTPRSYVRADGAVAPDHWLDPSDVWEVKCADLSLSPIYPAARGLVDSEKGISLRFPRFIRVREDKKPEQATTSAQVAGLYKKQSQIQNQQGAEPDSEQEEFY

Target ORF information:

RefSeq Version	XM_005219131.4
Organism	Bos taurus(cattle)
Definition	Bos taurus DNA ligase 1 (LIG1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005219131.4

ORF Insert Sequence:

ATGCAGCGAA GTATCATGTC ATTTTTCCAG CCCAAGAAAG AGGGCAAAGC GAAGAAGCCA 
GAGAAGGAGA CAGCCAAGAG CATCAGAGAA ACAGAGTCCC CTCCGAAGGT GGCGCTGAAG 
GAGCGGAATC GAGTGGTGTC TGAGGGTGAC TCTCCAGTGA AGAGGCCAGG GAGGAAGGCG 
ACCCAGGTCC TGGGCAGCGA GGGCGAGGAG GAGGAAGAGG AAGCCCCCAC CACCCCCAGA 
AGCCAGAAGC CTGCTTCAGA CTCTCCACAG GCCTCGCCTC CCAGTCCTGT CACACTGCCT 
GAGAGCAGCC CTTCCCACTC CAAACCCTCC CCAGCAGTTG CATCCCCATC AGGGATCCCG 
AAGCGTCGCA CGGCCCGGAA GCAGCTTCCC AAACGGACAT TTCAAGATGT TCTGAAAGAG 
CAGGGCGAGG ACAAGGATCC AGAAACTAAG AAGAAGAAGG AGGAAGAAGC GGAGACACCA 
ATAGAAAGCC TCCCAGAGCC TGAAGGGGAC GACAAGGAGG AAGTGGAAGG TGGGGACCAG 
CCCACGACGC CCCCTGAGCC CCGGAAGACC TCCAAGTCCC TGACAGAAAG CGTCTCAGAG 
CCCGGGGTGA CGGTGAAGCA GGAACCACAG GAGGATGGCC AGGCTCAACC GCCCCCCAAA 
ACTCCAAAGA CCCTCAGCAG TTTCTTCTCC CCCCGGAAGC CAGCTGTCAA AAAAGAAGCG 
AAAGAAGAAG GACCTGGTGC TCTGAGAAAG GACGAGATCA AGGGACACGT GGACCCAGCG 
AGCTATAATC CTGCCAAGAA CAACTATCAC CCCATCGAAG ATGCCTGCTG GAAGGCGGGC 
CAGAGGGTTC CTTATCTGGC TGTGGCCCGG GCGTTCGAGA AGATCGAGGA GGTCTCTGCT 
CGGCTCCGGA TGGTGGAGAC GCTGAGCAAC CTGCTGCGCT CCGTGGTGGC CCTGTCACCT 
GCTGACCTCC TGCCCGTCCT CTACCTGAGC CTCAACCGCC TGGGGCCGCC CCAGCAGGGC 
CTGGAGCTCG GCATCGGAGA GGGCATCCTC CTCAAGGCAG TGGCCCAGGC CACGGGTCGG 
CAGCTGGAGT CCGTCCGGGC CGAGGTGGCC GAGAAGGGTG ATGTGGGGCT GGTGGCCGAG 
AGCAGCCGCA GCACCCAGAG GCTCGTGCTG CCCCCGCCTG CGCTCACTGC CGCCGGCGTC 
TTCGCCAAGT TCCGGGACAT CGCGCAGCTG GCAGGCAGCG CTTCCACAAC CAAGAAGATT 
GACGTCATCA AAGGCCTCTT TGTGGCCTGC CGTCACTCAG AGGCACGTTT CATTGCCAGA 
GCCCTGAGTG GACGGCTGCG CCTCGGGTTG GCTGAGCAGT CGGTGCTGGC GGCCCTCGCC 
CAGGCCGTGA GCCTCACGCC CCCCAGTCAA GAATGCCCCC CCGCCGTGGT GGACGCCGGG 
AAGGGCAAGA CGGCAGAAGC CAGAAAGACG TGGCTGGAGG AGCAAGGCAT GATCCTGAAG 
CAGACTTTCT GTGAGGTACC TGACCTGGAG CGGATCGTCC CCGTGCTTCT GGAACACGGC 
CTGGAGCGGC TCCCGGAGCA CTGCAGACTG AGCCCAGGGG TTCCCCTGAA ACCAATGTTG 
GCCCACCCCA CCCGGGGTGT CAGTGAGGTC CTGAAGCGCT TTGAGGAGGC AGCTTTCACC 
TGCGAGTACA AATACGACGG GCAGAGGGCA CAGATCCACG TTCTGGAAGG CGGGGAGGTA 
AAGATCTTCA GCAGGAATCA GGAGGACAAT ACTGGAAGGT ACCCCGACAT TATCAGCCGC 
ATCCCTAAGA TTAAACTCCC ATCGGTCACA TCCTTCATCC TAGACACAGA AGCTGTAGCT 
TGGGACCGGG AAAAGAAGCA GATCCAGCCA TTCCAAGTCC TCACCACCCG CAAACGCAAG 
GAGGTGGACG CGGCGGAGAT CCAGGTGCAG GTGTGTCTGT ATGCCTTCGA TCTCATCTAC 
CTCAACGGAG AGTCCCTGGT ACGTGAGCCC CTCTCCCGAC GCCGGCAGCT GCTCCGGGAG 
AACTTCGTGG AGACGGAGGG CGAGTTCGTC TTTGCCACCT CCCTAGACAC CAAGGACACG 
GAGCAGATCG CAGAGTTCTT GGAGCAGTCG GTGAAAGACT CCTGCGAGGG GCTGATGGTG 
AAGACCCTGG ATGTGGACGC CACCTACGAG ATCGCCAAGA GGTCACACAA CTGGCTCAAG 
CTGAAGAAGG ACTACCTCGA GGGTGTGGGC GACACCCTGG ACCTCGTAGT GATTGGTGCC 
TACCTGGGCA AGGGGAAACG GGCCGGCCGG TACGGGGGCT TCCTGCTGGC CTCCTATGAC 
GAGGAGAGCG AGGAGTTCCA GGCCATCTGC AAGCTCGGAA CTGGCTTCAG TGACGAGGAG 
CTGGAAGAGC ACCACCAGAG GCTCCAGGCC TTAGTGCTGC CCACCCCGCG CTCCTACGTT 
CGGGCTGACG GTGCGGTGGC CCCCGACCAC TGGCTGGACC CCAGCGACGT GTGGGAGGTG 
AAGTGCGCGG ACCTCTCCCT GTCCCCCATC TACCCTGCGG CCCGAGGCCT GGTGGACAGC 
GAGAAGGGGA TCTCCCTCCG CTTCCCTCGG TTTATTCGCG TCCGTGAGGA TAAGAAGCCG 
GAGCAGGCCA CTACCAGTGC CCAGGTGGCC GGTCTGTACA AGAAGCAGAG TCAGATTCAG 
AACCAGCAGG GTGCGGAGCC GGACTCCGAG CAGGAAGAGT TCTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIG1 ( XM_010814982.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010813284.2 Bos taurus DNA ligase 1 (LIG1), transcript variant X4, mRNA.

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CloneID	OBa224762
Clone ID Related Accession (Same CDS sequence)	XM_010814982.3
Accession Version	XM_010814982.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2922bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	DNA ligase 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010814982.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGGC TTGGACCGCA ACCCGAAGGA CTCCTGGTGA CCAGTCCCGC TCTACTCTGC 
GGTTGCAGGA ACAGGAAACT GGTGACCAGC GGAGAGGAGG AGTGGGGAGA GAATTCTGAT 
ACCATCATGC AGCGAAGTAT CATGTCATTT TTCCAGCCCA AGAAAGAGGG CAAAGCGAAG 
AAGCCAGAGA AGGAGACAGC CAAGAGCATC AGAGAAACAG AGTCCCCTCC GAAGGTGGCG 
CTGAAGGAGC GGAATCGAGT GGTGTCTGAG GGTGACTCTC CAGTGAAGAG GCCAGGGAGG 
AAGGCGACCC AGGTCCTGGG CAGCGAGGGC GAGGAGGAGG AAGAGGAAGC CCCCACCACC 
CCCAGAAGCC AGAAGCCTGC TTCAGACTCT CCACAGGCCT CGCCTCCCAG TCCTGTCACA 
CTGCCTGAGA GCAGCCCTTC CCACTCCAAA CCCTCCCCAG CAGTTGCATC CCCATCAGGG 
ATCCCGAAGC GTCGCACGGC CCGGAAGCAG CTTCCCAAAC GGACATTTCA AGATGTTCTG 
AAAGAGCAGG GCGAGGACAA GGATCCAGAA ACTAAGAAGA AGAAGGAGGA AGAAGCGGAG 
ACACCAATAG AAAGCCTCCC AGAGCCTGAA GGGGACGACA AGGAGGAAGT GGAAGGTGGG 
GACCAGCCCA CGACGCCCCC TGAGCCCCGG AAGACCTCCA CAGAGTCCCT GACAGAAAGC 
GTCTCAGAGC CCGGGGTGAC GGTGAAGCAG GAACCACAGG AGGATGGCCA GGCTCAACCG 
CCCCCCAAAA CTCCAAAGAC CCTCAGCAGT TTCTTCTGTG AGTGGGGACT TCACCTTGCG 
GGGCTGCGCA GGGAAGAGGC AGCCCCCCGG AAGCCAGCTG TCAAAAAAGA AGCGAAAGAA 
GAAGGACCTG GTGCTCTGAG AAAGGACGAG ATCAAGGGAC ACGTGGACCC AGCGAGCTAT 
AATCCTGCCA AGAACAACTA TCACCCCATC GAAGATGCCT GCTGGAAGGC GGGCCAGAGG 
GTTCCTTATC TGGCTGTGGC CCGGGCGTTC GAGAAGATCG AGGAGGTCTC TGCTCGGCTC 
CGGATGGTGG AGACGCTGAG CAACCTGCTG CGCTCCGTGG TGGCCCTGTC ACCTGCTGAC 
CTCCTGCCCG TCCTCTACCT GAGCCTCAAC CGCCTGGGGC CGCCCCAGCA GGGCCTGGAG 
CTCGGCATCG GAGAGGGCAT CCTCCTCAAG GCAGTGGCCC AGGCCACGGG TCGGCAGCTG 
GAGTCCGTCC GGGCCGAGGT GGCCGAGAAG GGTGATGTGG GGCTGGTGGC CGAGAGCAGC 
CGCAGCACCC AGAGGCTCGT GCTGCCCCCG CCTGCGCTCA CTGCCGCCGG CGTCTTCGCC 
AAGTTCCGGG ACATCGCGCA GCTGGCAGGC AGCGCTTCCA CAACCAAGAA GATTGACGTC 
ATCAAAGGCC TCTTTGTGGC CTGCCGTCAC TCAGAGGCAC GTTTCATTGC CAGAGCCCTG 
AGTGGACGGC TGCGCCTCGG GTTGGCTGAG CAGTCGGTGC TGGCGGCCCT CGCCCAGGCC 
GTGAGCCTCA CGCCCCCCAG TCAAGAATGC CCCCCCGCCG TGGTGGACGC CGGGAAGGGC 
AAGACGGCAG AAGCCAGAAA GACGTGGCTG GAGGAGCAAG GCATGATCCT GAAGCAGACT 
TTCTGTGAGG TACCTGACCT GGAGCGGATC GTCCCCGTGC TTCTGGAACA CGGCCTGGAG 
CGGCTCCCGG AGCACTGCAG ACTGAGCCCA GGGGTTCCCC TGAAACCAAT GTTGGCCCAC 
CCCACCCGGG GTGTCAGTGA GGTCCTGAAG CGCTTTGAGG AGGCAGCTTT CACCTGCGAG 
TACAAATACG ACGGGCAGAG GGCACAGATC CACGTTCTGG AAGGCGGGGA GGTAAAGATC 
TTCAGCAGGA ATCAGGAGGA CAATACTGGA AGGTACCCCG ACATTATCAG CCGCATCCCT 
AAGATTAAAC TCCCATCGGT CACATCCTTC ATCCTAGACA CAGAAGCTGT AGCTTGGGAC 
CGGGAAAAGA AGCAGATCCA GCCATTCCAA GTCCTCACCA CCCGCAAACG CAAGGAGGTG 
GACGCGGCGG AGATCCAGGT GCAGGTGTGT CTGTATGCCT TCGATCTCAT CTACCTCAAC 
GGAGAGTCCC TGGTACGTGA GCCCCTCTCC CGACGCCGGC AGCTGCTCCG GGAGAACTTC 
GTGGAGACGG AGGGCGAGTT CGTCTTTGCC ACCTCCCTAG ACACCAAGGA CACGGAGCAG 
ATCGCAGAGT TCTTGGAGCA GTCGGTGAAA GACTCCTGCG AGGGGCTGAT GGTGAAGACC 
CTGGATGTGG ACGCCACCTA CGAGATCGCC AAGAGGTCAC ACAACTGGCT CAAGCTGAAG 
AAGGACTACC TCGAGGGTGT GGGCGACACC CTGGACCTCG TAGTGATTGG TGCCTACCTG 
GGCAAGGGGA AACGGGCCGG CCGGTACGGG GGCTTCCTGC TGGCCTCCTA TGACGAGGAG 
AGCGAGGAGT TCCAGGCCAT CTGCAAGCTC GGAACTGGCT TCAGTGACGA GGAGCTGGAA 
GAGCACCACC AGAGGCTCCA GGCCTTAGTG CTGCCCACCC CGCGCTCCTA CGTTCGGGCT 
GACGGTGCGG TGGCCCCCGA CCACTGGCTG GACCCCAGCG ACGTGTGGGA GGTGAAGTGC 
GCGGACCTCT CCCTGTCCCC CATCTACCCT GCGGCCCGAG GCCTGGTGGA CAGCGAGAAG 
GGGATCTCCC TCCGCTTCCC TCGGTTTATT CGCGTCCGTG AGGATAAGAA GCCGGAGCAG 
GCCACTACCA GTGCCCAGGT GGCCGGTCTG TACAAGAAGC AGAGTCAGAT TCAGAACCAG 
CAGGGTGCGG AGCCGGACTC CGAGCAGGAA GAGTTCTACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010813284.2
CDS	520..3441
Translation	MERLGPQPEGLLVTSPALLCGCRNRKLVTSGEEEWGENSDTIMQRSIMSFFQPKKEGKAKKPEKETAKSIRETESPPKVALKERNRVVSEGDSPVKRPGRKATQVLGSEGEEEEEEAPTTPRSQKPASDSPQASPPSPVTLPESSPSHSKPSPAVASPSGIPKRRTARKQLPKRTFQDVLKEQGEDKDPETKKKKEEEAETPIESLPEPEGDDKEEVEGGDQPTTPPEPRKTSTESLTESVSEPGVTVKQEPQEDGQAQPPPKTPKTLSSFFCEWGLHLAGLRREEAAPRKPAVKKEAKEEGPGALRKDEIKGHVDPASYNPAKNNYHPIEDACWKAGQRVPYLAVARAFEKIEEVSARLRMVETLSNLLRSVVALSPADLLPVLYLSLNRLGPPQQGLELGIGEGILLKAVAQATGRQLESVRAEVAEKGDVGLVAESSRSTQRLVLPPPALTAAGVFAKFRDIAQLAGSASTTKKIDVIKGLFVACRHSEARFIARALSGRLRLGLAEQSVLAALAQAVSLTPPSQECPPAVVDAGKGKTAEARKTWLEEQGMILKQTFCEVPDLERIVPVLLEHGLERLPEHCRLSPGVPLKPMLAHPTRGVSEVLKRFEEAAFTCEYKYDGQRAQIHVLEGGEVKIFSRNQEDNTGRYPDIISRIPKIKLPSVTSFILDTEAVAWDREKKQIQPFQVLTTRKRKEVDAAEIQVQVCLYAFDLIYLNGESLVREPLSRRRQLLRENFVETEGEFVFATSLDTKDTEQIAEFLEQSVKDSCEGLMVKTLDVDATYEIAKRSHNWLKLKKDYLEGVGDTLDLVVIGAYLGKGKRAGRYGGFLLASYDEESEEFQAICKLGTGFSDEELEEHHQRLQALVLPTPRSYVRADGAVAPDHWLDPSDVWEVKCADLSLSPIYPAARGLVDSEKGISLRFPRFIRVREDKKPEQATTSAQVAGLYKKQSQIQNQQGAEPDSEQEEFY

Target ORF information:

RefSeq Version	XM_010814982.3
Organism	Bos taurus(cattle)
Definition	Bos taurus DNA ligase 1 (LIG1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010814982.3

ORF Insert Sequence:

ATGGAGAGGC TTGGACCGCA ACCCGAAGGA CTCCTGGTGA CCAGTCCCGC TCTACTCTGC 
GGTTGCAGGA ACAGGAAACT GGTGACCAGC GGAGAGGAGG AGTGGGGAGA GAATTCTGAT 
ACCATCATGC AGCGAAGTAT CATGTCATTT TTCCAGCCCA AGAAAGAGGG CAAAGCGAAG 
AAGCCAGAGA AGGAGACAGC CAAGAGCATC AGAGAAACAG AGTCCCCTCC GAAGGTGGCG 
CTGAAGGAGC GGAATCGAGT GGTGTCTGAG GGTGACTCTC CAGTGAAGAG GCCAGGGAGG 
AAGGCGACCC AGGTCCTGGG CAGCGAGGGC GAGGAGGAGG AAGAGGAAGC CCCCACCACC 
CCCAGAAGCC AGAAGCCTGC TTCAGACTCT CCACAGGCCT CGCCTCCCAG TCCTGTCACA 
CTGCCTGAGA GCAGCCCTTC CCACTCCAAA CCCTCCCCAG CAGTTGCATC CCCATCAGGG 
ATCCCGAAGC GTCGCACGGC CCGGAAGCAG CTTCCCAAAC GGACATTTCA AGATGTTCTG 
AAAGAGCAGG GCGAGGACAA GGATCCAGAA ACTAAGAAGA AGAAGGAGGA AGAAGCGGAG 
ACACCAATAG AAAGCCTCCC AGAGCCTGAA GGGGACGACA AGGAGGAAGT GGAAGGTGGG 
GACCAGCCCA CGACGCCCCC TGAGCCCCGG AAGACCTCCA CAGAGTCCCT GACAGAAAGC 
GTCTCAGAGC CCGGGGTGAC GGTGAAGCAG GAACCACAGG AGGATGGCCA GGCTCAACCG 
CCCCCCAAAA CTCCAAAGAC CCTCAGCAGT TTCTTCTGTG AGTGGGGACT TCACCTTGCG 
GGGCTGCGCA GGGAAGAGGC AGCCCCCCGG AAGCCAGCTG TCAAAAAAGA AGCGAAAGAA 
GAAGGACCTG GTGCTCTGAG AAAGGACGAG ATCAAGGGAC ACGTGGACCC AGCGAGCTAT 
AATCCTGCCA AGAACAACTA TCACCCCATC GAAGATGCCT GCTGGAAGGC GGGCCAGAGG 
GTTCCTTATC TGGCTGTGGC CCGGGCGTTC GAGAAGATCG AGGAGGTCTC TGCTCGGCTC 
CGGATGGTGG AGACGCTGAG CAACCTGCTG CGCTCCGTGG TGGCCCTGTC ACCTGCTGAC 
CTCCTGCCCG TCCTCTACCT GAGCCTCAAC CGCCTGGGGC CGCCCCAGCA GGGCCTGGAG 
CTCGGCATCG GAGAGGGCAT CCTCCTCAAG GCAGTGGCCC AGGCCACGGG TCGGCAGCTG 
GAGTCCGTCC GGGCCGAGGT GGCCGAGAAG GGTGATGTGG GGCTGGTGGC CGAGAGCAGC 
CGCAGCACCC AGAGGCTCGT GCTGCCCCCG CCTGCGCTCA CTGCCGCCGG CGTCTTCGCC 
AAGTTCCGGG ACATCGCGCA GCTGGCAGGC AGCGCTTCCA CAACCAAGAA GATTGACGTC 
ATCAAAGGCC TCTTTGTGGC CTGCCGTCAC TCAGAGGCAC GTTTCATTGC CAGAGCCCTG 
AGTGGACGGC TGCGCCTCGG GTTGGCTGAG CAGTCGGTGC TGGCGGCCCT CGCCCAGGCC 
GTGAGCCTCA CGCCCCCCAG TCAAGAATGC CCCCCCGCCG TGGTGGACGC CGGGAAGGGC 
AAGACGGCAG AAGCCAGAAA GACGTGGCTG GAGGAGCAAG GCATGATCCT GAAGCAGACT 
TTCTGTGAGG TACCTGACCT GGAGCGGATC GTCCCCGTGC TTCTGGAACA CGGCCTGGAG 
CGGCTCCCGG AGCACTGCAG ACTGAGCCCA GGGGTTCCCC TGAAACCAAT GTTGGCCCAC 
CCCACCCGGG GTGTCAGTGA GGTCCTGAAG CGCTTTGAGG AGGCAGCTTT CACCTGCGAG 
TACAAATACG ACGGGCAGAG GGCACAGATC CACGTTCTGG AAGGCGGGGA GGTAAAGATC 
TTCAGCAGGA ATCAGGAGGA CAATACTGGA AGGTACCCCG ACATTATCAG CCGCATCCCT 
AAGATTAAAC TCCCATCGGT CACATCCTTC ATCCTAGACA CAGAAGCTGT AGCTTGGGAC 
CGGGAAAAGA AGCAGATCCA GCCATTCCAA GTCCTCACCA CCCGCAAACG CAAGGAGGTG 
GACGCGGCGG AGATCCAGGT GCAGGTGTGT CTGTATGCCT TCGATCTCAT CTACCTCAAC 
GGAGAGTCCC TGGTACGTGA GCCCCTCTCC CGACGCCGGC AGCTGCTCCG GGAGAACTTC 
GTGGAGACGG AGGGCGAGTT CGTCTTTGCC ACCTCCCTAG ACACCAAGGA CACGGAGCAG 
ATCGCAGAGT TCTTGGAGCA GTCGGTGAAA GACTCCTGCG AGGGGCTGAT GGTGAAGACC 
CTGGATGTGG ACGCCACCTA CGAGATCGCC AAGAGGTCAC ACAACTGGCT CAAGCTGAAG 
AAGGACTACC TCGAGGGTGT GGGCGACACC CTGGACCTCG TAGTGATTGG TGCCTACCTG 
GGCAAGGGGA AACGGGCCGG CCGGTACGGG GGCTTCCTGC TGGCCTCCTA TGACGAGGAG 
AGCGAGGAGT TCCAGGCCAT CTGCAAGCTC GGAACTGGCT TCAGTGACGA GGAGCTGGAA 
GAGCACCACC AGAGGCTCCA GGCCTTAGTG CTGCCCACCC CGCGCTCCTA CGTTCGGGCT 
GACGGTGCGG TGGCCCCCGA CCACTGGCTG GACCCCAGCG ACGTGTGGGA GGTGAAGTGC 
GCGGACCTCT CCCTGTCCCC CATCTACCCT GCGGCCCGAG GCCTGGTGGA CAGCGAGAAG 
GGGATCTCCC TCCGCTTCCC TCGGTTTATT CGCGTCCGTG AGGATAAGAA GCCGGAGCAG 
GCCACTACCA GTGCCCAGGT GGCCGGTCTG TACAAGAAGC AGAGTCAGAT TCAGAACCAG 
CAGGGTGCGG AGCCGGACTC CGAGCAGGAA GAGTTCTACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	LIG1
Entrez Gene ID	100124507
Full Name	DNA ligase 1
General protein information	Preferred Names DNA ligase 1 Names DNA ligase 1 DNA ligase (ATP) 1 DNA ligase I
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Homo sapiens (human)	LIG1	NP_000225.1
Danio rerio (zebrafish)	si:dkeyp-35b8.5	NP_001119860.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009222	XP_319999.3
Saccharomyces cerevisiae (baker's yeast)	CDC9	NP_010117.1
Arabidopsis thaliana (thale cress)	AT1G49250	NP_175351.1
	LIG1	XP_002801366.1
Rattus norvegicus (Norway rat)	Lig1	NP_001019439.1
Gallus gallus (chicken)	LIG1	XP_003643178.2
Drosophila melanogaster (fruit fly)	DNA-ligI	NP_611843.2
Caenorhabditis elegans (roundworm)	lig-1	NP_741625.2
Eremothecium gossypii	AGOS_ACL155W	NP_983249.1
Oryza sativa (rice)	Os10g0489200	NP_001064917.1
Xenopus tropicalis (tropical clawed frog)	lig1	XP_002941929.1
Bos taurus (cattle)	LIG1	NP_001096018.1
Kluyveromyces lactis	KLLA0D12496g	XP_453619.1
Mus musculus (house mouse)	Lig1	NP_001076657.1
Magnaporthe oryzae (rice blast fungus)	MGG_06370	XP_003717204.1
Arabidopsis thaliana (thale cress)	LIG1	NP_172293.2
Pan troglodytes (chimpanzee)	LIG1	XP_001170212.3
Canis lupus familiaris (dog)	LIG1	XP_005616432.1
Rattus norvegicus (Norway rat)	LOC100911727	XP_003748816.2
Schizosaccharomyces pombe (fission yeast)	cdc17	NP_593318.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

LIG1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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ORF Insert Sequence:

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Target ORF information:

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