Lig1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Lig1
Entrez Gene ID	81513
Full Name	DNA ligase 1
General protein information	Preferred Names DNA ligase 1 Names DNA ligase 1 DNA ligase (ATP) 1 DNA ligase I ligase I, DNA, ATP-dependent polydeoxyribonucleotide synthase [ATP] 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q21
Summary	catalyzes the joining of blunt ended DNA [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001024268.2	NP_001019439.1	DNA ligase 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	LIG1	NP_000225.1
Danio rerio (zebrafish)	si:dkeyp-35b8.5	NP_001119860.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009222	XP_319999.3
Saccharomyces cerevisiae (baker's yeast)	CDC9	NP_010117.1
Arabidopsis thaliana (thale cress)	AT1G49250	NP_175351.1
	LIG1	XP_002801366.1
Rattus norvegicus (Norway rat)	Lig1	NP_001019439.1
Gallus gallus (chicken)	LIG1	XP_003643178.2
Drosophila melanogaster (fruit fly)	DNA-ligI	NP_611843.2
Caenorhabditis elegans (roundworm)	lig-1	NP_741625.2
Eremothecium gossypii	AGOS_ACL155W	NP_983249.1
Oryza sativa (rice)	Os10g0489200	NP_001064917.1
Xenopus tropicalis (tropical clawed frog)	lig1	XP_002941929.1
Bos taurus (cattle)	LIG1	NP_001096018.1
Kluyveromyces lactis	KLLA0D12496g	XP_453619.1
Mus musculus (house mouse)	Lig1	NP_001076657.1
Magnaporthe oryzae (rice blast fungus)	MGG_06370	XP_003717204.1
Arabidopsis thaliana (thale cress)	LIG1	NP_172293.2
Pan troglodytes (chimpanzee)	LIG1	XP_001170212.3
Canis lupus familiaris (dog)	LIG1	XP_005616432.1
Rattus norvegicus (Norway rat)	LOC100911727	XP_003748816.2
Schizosaccharomyces pombe (fission yeast)	cdc17	NP_593318.2

Gene Ontology
Bibliography

Related articles in PubMed

DNA polymerases and repair synthesis in NER in human cells.
Lehmann AR
DNA repair10(7)730-3(2011 Jul)

DNA ligase III promotes alternative nonhomologous end-joining during chromosomal translocation formation.
Simsek D, Brunet E, Wong SY, Katyal S, Gao Y, McKinnon PJ, Lou J, Zhang L, Li J, Rebar EJ, Gregory PD, Holmes MC, Jasin M
PLoS genetics7(6)e1002080(2011 Jun)

DNA ligase III is critical for mtDNA integrity but not Xrcc1-mediated nuclear DNA repair.
Gao Y, Katyal S, Lee Y, Zhao J, Rehg JE, Russell HR, McKinnon PJ
Nature471(7337)240-4(2011 Mar)

Distinct kinetics of human DNA ligases I, IIIalpha, IIIbeta, and IV reveal direct DNA sensing ability and differential physiological functions in DNA repair.
Chen X, Ballin JD, Della-Maria J, Tsai MS, White EJ, Tomkinson AE, Wilson GM
DNA repair8(8)961-8(2009 Aug)

Mechanisms and functions of DNA mismatch repair.
Li GM
Cell research18(1)85-98(2008 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Lig1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Lig1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12240	NM_001024268.2 Latest version!	Rattus norvegicus DNA ligase 1 (Lig1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$538.30 $769.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Lig1 ( NM_001024268.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001019439.1 Rattus norvegicus DNA ligase 1 (Lig1), mRNA.

Price & Availability↑

CloneID	ORa12240
Clone ID Related Accession (Same CDS sequence)	NM_001024268.2
Accession Version	NM_001024268.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2742bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	DNA ligase 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from FQ132357.1, BC093604.1 and BE104054.1. On or before Aug 4, 2012 this sequence version replaced NM_030855.1, NM_001024268.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF276774.1, BC093604.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved ##RefSeq-Attributes-END##

ATGCAGAGAA GTATTATGTC ATTTTTCCAA CCCACAACAA CAGAAGGTAA AGCGAAGAAG 
CCAGAGAAGG AGATACCCAG CAGCATCAGA GAGAAGGAAC CCCCTCCAAA GGTGGCATTG 
AAGGAGAGGA ATCGAGCAGT GCCTGAGAGT GATTCTCCAG TGAAGAGGCC AGGAAGGAAG 
GTGGCCCAGG TTCTGAGCAG TGAAGGGGAG GACGAAGATG AAGCCCCTGG CACCCCCCAA 
GTCCAAAAGC CTGTGTCAGA CTCCAAACAG AGCTCTCCTC CCAGCCCTGA CTCCTGTCCT 
GAGAACAGCA GCCCCTCCAT GGACATCTCC CCATCAGGAT TCCCAAAGCG CCGAACTGCA 
CGGAAGCAGC TCCCAAAACG GACAATTCAA GACACTCTTG AGGAGCACAA TGAAGACAAA 
GACAAAGCAG CTAAGAAAAG GAAAAAAGAA GATACACAGA CCCCAACAGA AAGCCTCACA 
GAGGCTGAAG AAGTAAAACA GAAGGAAGAA CAGGTGGAGG ACCAGCCCAC AGTACCCCCT 
GAGCCCACAG AGTCCCCTGA GTCAGTAACC CTCACAAAGA CAGAGAACAT TCCAATGTGT 
AAGGCAGGTG TGAAACAGAA GCCTCAGGAA GAAGAACAGA GCAAGCCTCC TGCCAGGGGT 
GCCAAGCCAC TGAGCAGTTT CTTCACACCC CGAAAACCAG CAGTGAAAAC TGAAGTCAAA 
CAAGAAGAGT CAGACACTCC AAGGAAGGAA GAGACCAAGG GAGCCCCGGA TCCAACCAAT 
TATAATCCTT CCAAGAGTAA CTACCATCCC ATTGAAGATG CCTGCTGGAA ACATGGCCAA 
AAGGTTCCTT TTCTTGCTGT GGCCCGGACA TTTGAGAAGA TTGAGGAGGT GTCTGCTCGG 
CTTAAGATGG TGGAGACACT GAGCAACCTG CTGCGCTCCG TGGTGGCCCT GTCCCCAACA 
GACCTGCTCC CTGTTCTCTA TCTCAGCCTC AACCGCCTTG GGCCGCCTCA GCAGGGTCTA 
GAACTGGGTG TTGGTGATGG TGTCCTCCTC AAGGCAGTTG CCCAGGCCAC AGGTCGTCAG 
CTGGAGTCCA TCCGGGCTGA GGTAGCTGAG AAGGGGGATG TGGGGCTAGT GGCGGAGAAC 
AGCCGAAGCA CTCAGAGGCT CATGCTGCCT CCACCTCCAC TTACTGTTTC TGGAGTCTTT 
ACCAAATTCT GTGACATTGC CCGGCTCACT GGCAGTGCTT CCATGGCCAA GAAAATGGAC 
ATTATCAAGG GCCTTTTTGT TGCCTGCCGC TACTCAGAAG CCCGGTTTAT TGCCAGGTCC 
CTAAGTGGGC GGCTGCGCCT TGGGCTGGCT GAGCAGTCAG TACTGGCTGC TCTTGCCCAG 
GCTGTGAGCC TCACACCCCC TGGCCAAGAA TTTCCCACAG CCGTGGTGGA TGCTGGGAAG 
GGCAAGACAG CAGAGGCCAG AAAGATGTGG TTGGAAGAAC AAGGCATGAT CTTGAAGCAA 
ACCTTCTGTG AGGTGCCTGA TCTGGATCGC ATCATCCCTG TGCTGCTGGA ACATGGCTTG 
GAAAGTCTCC CAGAGCACTG CAAGCTGAGC CCAGGGGTTC CTCTTAAACC AATGCTGGCC 
CATCCCACTC GGGGTGTCCG TGAGGTCCTG AAACGCTTTG AGGAGGTCGA CTTCACCTGC 
GAGTATAAAT ACGATGGGCA GCGGGCACAG ATACATGTTC TGGAAGGTGG AGAAGTGAAG 
ATCTTCAGCA GGAACCAGGA AGACAACTCA GGAAAGTATC CTGACATCAT CAGCCGCATC 
CCCAAGATTA AACACCCCTC AGTCACATCT TTCATTCTGG ACACTGAGGC TGTGGCCTGG 
GACCGGGAAA AGAAGCAGAT CCAGCCATTC CAAGTGCTCA CCACACGCAA GCGCAAGGAG 
GTTGATGCAT CGGAGATACA GGTACAAGTG TGTCTGTATG CTTTTGACCT CATCTACCTC 
AACGGAGAGT CCCTGGCTCG CCAGCCCCTG TCTCGACGCC GGCAGCTACT CCGTGAGAAC 
TTTGTGGAGA CAGAGGGTGA GTTTGTCTTT GCCACCTCCT TGGACACCAA GGACATCGAG 
CAGATTGCCG AGTTCTTAGA GCAGTCAGTG AAGGACTCCT GTGAGGGCCT GATGGTGAAG 
ACCCTGGATG TTGATGCTAC CTATGAGATC GCCAAGAGGT CACACAACTG GCTCAAGCTG 
AAGAAGGACT ACCTTGAAGG TGTGGGCGAC ACTCTAGACC TTGTGGTGAT TGGTGCCTAC 
CTGGGCCGGG GGAAGCGGGC TGGCCGGTAT GGGGGCTTCC TGTTGGCTGC CTACGATGAG 
GAGAGTGAAG AGCTGCAGGC CATATGCAAG CTGGGAACCG GATTCAGCGA CGAGGAGCTG 
GAAGAACATC ATCAGAGCAT GCAGGCCCTT TTATTGCCTA CCCCACGCCC CTATGTGAGG 
ATTGATGGGG CAGTGGCCCC AGACCACTGG CTAGACCCGA GTGTTGTGTG GGAGGTGAAA 
TGTGCTGATC TCTCCCTGTC TCCCATCTAC CCTGCTGCTC GGGGCCTGGT GGACAAAGAA 
AAAGGGATCT CCCTTCGTTT CCCTCGGTTC ATTCGTGTCC GTGAGGATAA GCAGCCAGAG 
CAGGCCACTA CCAGTGACCA GGTGGCCTCT TTGTACCGGA AGCAGAGTCA GATTCAGAAC 
CAGCAAAGCT CAGACTTGGA CTCTGATGTT GAAGATTACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001019439.1
CDS	164..2905
Translation	MQRSIMSFFQPTTTEGKAKKPEKEIPSSIREKEPPPKVALKERNRAVPESDSPVKRPGRKVAQVLSSEGEDEDEAPGTPQVQKPVSDSKQSSPPSPDSCPENSSPSMDISPSGFPKRRTARKQLPKRTIQDTLEEHNEDKDKAAKKRKKEDTQTPTESLTEAEEVKQKEEQVEDQPTVPPEPTESPESVTLTKTENIPMCKAGVKQKPQEEEQSKPPARGAKPLSSFFTPRKPAVKTEVKQEESDTPRKEETKGAPDPTNYNPSKSNYHPIEDACWKHGQKVPFLAVARTFEKIEEVSARLKMVETLSNLLRSVVALSPTDLLPVLYLSLNRLGPPQQGLELGVGDGVLLKAVAQATGRQLESIRAEVAEKGDVGLVAENSRSTQRLMLPPPPLTVSGVFTKFCDIARLTGSASMAKKMDIIKGLFVACRYSEARFIARSLSGRLRLGLAEQSVLAALAQAVSLTPPGQEFPTAVVDAGKGKTAEARKMWLEEQGMILKQTFCEVPDLDRIIPVLLEHGLESLPEHCKLSPGVPLKPMLAHPTRGVREVLKRFEEVDFTCEYKYDGQRAQIHVLEGGEVKIFSRNQEDNSGKYPDIISRIPKIKHPSVTSFILDTEAVAWDREKKQIQPFQVLTTRKRKEVDASEIQVQVCLYAFDLIYLNGESLARQPLSRRRQLLRENFVETEGEFVFATSLDTKDIEQIAEFLEQSVKDSCEGLMVKTLDVDATYEIAKRSHNWLKLKKDYLEGVGDTLDLVVIGAYLGRGKRAGRYGGFLLAAYDEESEELQAICKLGTGFSDEELEEHHQSMQALLLPTPRPYVRIDGAVAPDHWLDPSVVWEVKCADLSLSPIYPAARGLVDKEKGISLRFPRFIRVREDKQPEQATTSDQVASLYRKQSQIQNQQSSDLDSDVEDY

Target ORF information:

RefSeq Version	NM_001024268.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DNA ligase 1 (Lig1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001024268.2

ORF Insert Sequence:

ATGCAGAGAA GTATTATGTC ATTTTTCCAA CCCACAACAA CAGAAGGTAA AGCGAAGAAG 
CCAGAGAAGG AGATACCCAG CAGCATCAGA GAGAAGGAAC CCCCTCCAAA GGTGGCATTG 
AAGGAGAGGA ATCGAGCAGT GCCTGAGAGT GATTCTCCAG TGAAGAGGCC AGGAAGGAAG 
GTGGCCCAGG TTCTGAGCAG TGAAGGGGAG GACGAAGATG AAGCCCCTGG CACCCCCCAA 
GTCCAAAAGC CTGTGTCAGA CTCCAAACAG AGCTCTCCTC CCAGCCCTGA CTCCTGTCCT 
GAGAACAGCA GCCCCTCCAT GGACATCTCC CCATCAGGAT TCCCAAAGCG CCGAACTGCA 
CGGAAGCAGC TCCCAAAACG GACAATTCAA GACACTCTTG AGGAGCACAA TGAAGACAAA 
GACAAAGCAG CTAAGAAAAG GAAAAAAGAA GATACACAGA CCCCAACAGA AAGCCTCACA 
GAGGCTGAAG AAGTAAAACA GAAGGAAGAA CAGGTGGAGG ACCAGCCCAC AGTACCCCCT 
GAGCCCACAG AGTCCCCTGA GTCAGTAACC CTCACAAAGA CAGAGAACAT TCCAATGTGT 
AAGGCAGGTG TGAAACAGAA GCCTCAGGAA GAAGAACAGA GCAAGCCTCC TGCCAGGGGT 
GCCAAGCCAC TGAGCAGTTT CTTCACACCC CGAAAACCAG CAGTGAAAAC TGAAGTCAAA 
CAAGAAGAGT CAGACACTCC AAGGAAGGAA GAGACCAAGG GAGCCCCGGA TCCAACCAAT 
TATAATCCTT CCAAGAGTAA CTACCATCCC ATTGAAGATG CCTGCTGGAA ACATGGCCAA 
AAGGTTCCTT TTCTTGCTGT GGCCCGGACA TTTGAGAAGA TTGAGGAGGT GTCTGCTCGG 
CTTAAGATGG TGGAGACACT GAGCAACCTG CTGCGCTCCG TGGTGGCCCT GTCCCCAACA 
GACCTGCTCC CTGTTCTCTA TCTCAGCCTC AACCGCCTTG GGCCGCCTCA GCAGGGTCTA 
GAACTGGGTG TTGGTGATGG TGTCCTCCTC AAGGCAGTTG CCCAGGCCAC AGGTCGTCAG 
CTGGAGTCCA TCCGGGCTGA GGTAGCTGAG AAGGGGGATG TGGGGCTAGT GGCGGAGAAC 
AGCCGAAGCA CTCAGAGGCT CATGCTGCCT CCACCTCCAC TTACTGTTTC TGGAGTCTTT 
ACCAAATTCT GTGACATTGC CCGGCTCACT GGCAGTGCTT CCATGGCCAA GAAAATGGAC 
ATTATCAAGG GCCTTTTTGT TGCCTGCCGC TACTCAGAAG CCCGGTTTAT TGCCAGGTCC 
CTAAGTGGGC GGCTGCGCCT TGGGCTGGCT GAGCAGTCAG TACTGGCTGC TCTTGCCCAG 
GCTGTGAGCC TCACACCCCC TGGCCAAGAA TTTCCCACAG CCGTGGTGGA TGCTGGGAAG 
GGCAAGACAG CAGAGGCCAG AAAGATGTGG TTGGAAGAAC AAGGCATGAT CTTGAAGCAA 
ACCTTCTGTG AGGTGCCTGA TCTGGATCGC ATCATCCCTG TGCTGCTGGA ACATGGCTTG 
GAAAGTCTCC CAGAGCACTG CAAGCTGAGC CCAGGGGTTC CTCTTAAACC AATGCTGGCC 
CATCCCACTC GGGGTGTCCG TGAGGTCCTG AAACGCTTTG AGGAGGTCGA CTTCACCTGC 
GAGTATAAAT ACGATGGGCA GCGGGCACAG ATACATGTTC TGGAAGGTGG AGAAGTGAAG 
ATCTTCAGCA GGAACCAGGA AGACAACTCA GGAAAGTATC CTGACATCAT CAGCCGCATC 
CCCAAGATTA AACACCCCTC AGTCACATCT TTCATTCTGG ACACTGAGGC TGTGGCCTGG 
GACCGGGAAA AGAAGCAGAT CCAGCCATTC CAAGTGCTCA CCACACGCAA GCGCAAGGAG 
GTTGATGCAT CGGAGATACA GGTACAAGTG TGTCTGTATG CTTTTGACCT CATCTACCTC 
AACGGAGAGT CCCTGGCTCG CCAGCCCCTG TCTCGACGCC GGCAGCTACT CCGTGAGAAC 
TTTGTGGAGA CAGAGGGTGA GTTTGTCTTT GCCACCTCCT TGGACACCAA GGACATCGAG 
CAGATTGCCG AGTTCTTAGA GCAGTCAGTG AAGGACTCCT GTGAGGGCCT GATGGTGAAG 
ACCCTGGATG TTGATGCTAC CTATGAGATC GCCAAGAGGT CACACAACTG GCTCAAGCTG 
AAGAAGGACT ACCTTGAAGG TGTGGGCGAC ACTCTAGACC TTGTGGTGAT TGGTGCCTAC 
CTGGGCCGGG GGAAGCGGGC TGGCCGGTAT GGGGGCTTCC TGTTGGCTGC CTACGATGAG 
GAGAGTGAAG AGCTGCAGGC CATATGCAAG CTGGGAACCG GATTCAGCGA CGAGGAGCTG 
GAAGAACATC ATCAGAGCAT GCAGGCCCTT TTATTGCCTA CCCCACGCCC CTATGTGAGG 
ATTGATGGGG CAGTGGCCCC AGACCACTGG CTAGACCCGA GTGTTGTGTG GGAGGTGAAA 
TGTGCTGATC TCTCCCTGTC TCCCATCTAC CCTGCTGCTC GGGGCCTGGT GGACAAAGAA 
AAAGGGATCT CCCTTCGTTT CCCTCGGTTC ATTCGTGTCC GTGAGGATAA GCAGCCAGAG 
CAGGCCACTA CCAGTGACCA GGTGGCCTCT TTGTACCGGA AGCAGAGTCA GATTCAGAAC 
CAGCAAAGCT CAGACTTGGA CTCTGATGTT GAAGATTACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

DNA polymerases and repair synthesis in NER in human cells.
DNA repair10(7)730-3(2011 Jul)
Lehmann AR

DNA ligase III promotes alternative nonhomologous end-joining during chromosomal translocation formation.
PLoS genetics7(6)e1002080(2011 Jun)
Simsek D,Brunet E,Wong SY,Katyal S,Gao Y,McKinnon PJ,Lou J,Zhang L,Li J,Rebar EJ,Gregory PD,Holmes MC,Jasin M

DNA ligase III is critical for mtDNA integrity but not Xrcc1-mediated nuclear DNA repair.
Nature471(7337)240-4(2011 Mar)
Gao Y,Katyal S,Lee Y,Zhao J,Rehg JE,Russell HR,McKinnon PJ

Distinct kinetics of human DNA ligases I, IIIalpha, IIIbeta, and IV reveal direct DNA sensing ability and differential physiological functions in DNA repair.
DNA repair8(8)961-8(2009 Aug)
Chen X,Ballin JD,Della-Maria J,Tsai MS,White EJ,Tomkinson AE,Wilson GM

Mechanisms and functions of DNA mismatch repair.
Cell research18(1)85-98(2008 Jan)
Li GM

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Lig1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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