DNAlig1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	DNAlig1
Entrez Gene ID	37791
Full Name	DNA ligase I
Synonyms	CG5602,DmDNA Lig I,Dmel\CG5602,LIG1
General protein information	Preferred Names DNA ligase I Names CG5602 gene product from transcript CG5602-RA CG5602-PA DNA-ligI-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 60A8-60A8

mRNA and Protein(s)

mRNA	Protein	Name
NM_137999.3	NP_611843.2	DNA ligase 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	LIG1	NP_000225.1
Danio rerio (zebrafish)	si:dkeyp-35b8.5	NP_001119860.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009222	XP_319999.3
Saccharomyces cerevisiae (baker's yeast)	CDC9	NP_010117.1
Arabidopsis thaliana (thale cress)	AT1G49250	NP_175351.1
	LIG1	XP_002801366.1
Rattus norvegicus (Norway rat)	Lig1	NP_001019439.1
Gallus gallus (chicken)	LIG1	XP_003643178.2
Drosophila melanogaster (fruit fly)	DNA-ligI	NP_611843.2
Caenorhabditis elegans (roundworm)	lig-1	NP_741625.2
Eremothecium gossypii	AGOS_ACL155W	NP_983249.1
Oryza sativa (rice)	Os10g0489200	NP_001064917.1
Xenopus tropicalis (tropical clawed frog)	lig1	XP_002941929.1
Bos taurus (cattle)	LIG1	NP_001096018.1
Kluyveromyces lactis	KLLA0D12496g	XP_453619.1
Mus musculus (house mouse)	Lig1	NP_001076657.1
Magnaporthe oryzae (rice blast fungus)	MGG_06370	XP_003717204.1
Arabidopsis thaliana (thale cress)	LIG1	NP_172293.2
Pan troglodytes (chimpanzee)	LIG1	XP_001170212.3
Canis lupus familiaris (dog)	LIG1	XP_005616432.1
Rattus norvegicus (Norway rat)	LOC100911727	XP_003748816.2
Schizosaccharomyces pombe (fission yeast)	cdc17	NP_593318.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following DNAlig1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DNAlig1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa13206	NM_137999.3 Latest version!	Drosophila melanogaster DNA ligase 1 (DNAlig1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DNAlig1 ( NM_137999.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_611843.2 Drosophila melanogaster DNA ligase 1 (DNAlig1), mRNA.

Price & Availability↑

CloneID	OFa13206
Clone ID Related Accession (Same CDS sequence)	NM_137999.3
Accession Version	NM_137999.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	DNA ligase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_137999.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAAAAGT CTATAACCTC CTTCTTCAAG AAGAAATCCG ATGCCACCGA CAGCCCCTCG 
CCGCCCAAGA AAGTTCCTAA GATTGATGCG AAGACCGAAC TCCCAGATGA ACCCCACATC 
AAGAGCGAAA GTGCCTCCCC GGAAACTAAG CCCAAGGTAG AACCTATGTC TGTGGATTCC 
GAGGAGAAGA CAAGTCCGGT GAAAAATGTT AAGAAGGAAC CAAAGGAAGT CGATGACAAG 
ACCACTGACA AGAAGGTCAC AACCATAGGT CTCAACTCCA CTGCCGCTAC TAAGGAGGAT 
GTGGAAAACT ACGATCCATC CGCCGACTCC TATCATCCGC TCAAGAATGC CTACTGGAAG 
GACAAAAAGG TCACTCCCTA CTTGGCACTG GCCCGCACAT TTCAAGTAAT CGAGGAGACC 
AAAGGCCGGC TCAAGATGAT CGACACGCTG AGCAACTTCT TCTGCTCTGT GATGCTGGTC 
AGTCCGGAGG ATCTTGTGCC CAGCGTCTAC CTCAGCATCA ACCAACTGGC GCCCGCCTAC 
GAGGGACTGG AGCTGGGCGT TGCCGAGACC ACCTTGATGA AGGCCATTTG CAAGGCAACG 
GGGCGTAATT TGGCTCACAT TAAATCGCAG ACGCAGCTAA CCGGGGATCT GGGCATCGTG 
GCCGAGCAGT CGCGCGTTTC ACAGCGCATG ATGTTCCAGC CAGCGCCTCT GAATGTGAGG 
GATGTGTTCC GGAAGCTACG CGAAATAGCC AAGCTATCCG GTCAGTCGAA AATGGACCTG 
GTGTACAATA TGTTTGTGGC CTGTCGATCG TCGGAGGCTC GCTTCTTTAT CCGTTCATTG 
ATTGGCAAGC TAAGAATTGG CATTGCAGAG CAGAGTTTGC TCACAGCGCT GGCCATTGGT 
TTGGTGAAAA AGAACCACAT CGATGATTGC AAGGCTAGCA AGGTGCCGGA TGTGTATAAG 
GATGAAATAG TCGACACCAC TTTGCTGTTG AAAACTGCAT ACTGTCAGTG TCCCAACTAC 
GATATCATTA TACCGGCCAT TCTAAAGTAC GATATTAAGG AGCTGCAGGA ACGCTGCCCC 
ATGCATCCCG GCATGCCGCT GAGGCCCATG CTCGCCCAGC CCACCAAGGG GGTCCATGAG 
GTATTTGAGA GATTCGGCGG CATGCAAATC ACCTGCGAGT GGAAATACGA TGGAGAGCGG 
GCCCAAATCC ACCGCAACGA GAAGGGGGAG ATCAGCATAT TCTCGCGCAA CTCCGAGAAC 
AACACGGCAA AATATCCGGA TTTGATTGCC AGGAGCACTG CCTTGCTCAA GGGCGACGTA 
AAGTCCTACA TCATAGACAG CGAAATTGTG GCCTGGGATG TGGAGCGCAA GCAGATTCTG 
CCGTTTCAGG TGCTCAGCAC GCGCAAACGG AAGAACGTTG ATATCGAGGA AATCAAGGTC 
CAAGTCTGTG TCTACATCTT CGATCTGCTG TACATCAATG GCACTGCTCT GGTGACCAAA 
AACCTGTCGG AGCGTCGCAA GCTCCTGTTG GAGCACTTTC AAGAGGTCGA GGGCGAGTGG 
AAGTTCGCCA CTGCTCTGGA CACCAATGAC ATAGACGAAG TGCAGCAGTT CCTGGAGGAG 
TCTATCAAAG GAAATTGCGA GGGCCTCATG GTCAAAACAT TGGATGAGGA GGCCACCTAT 
GAGATTGCCA AGAGATCCCG CAACTGGCTG AAATTGAAGA AGGACTACCT ATCCAATGTG 
GGCGACTCAC TCGATTTGGT GGTCATTGGT GGCTACAAAG GCAAGGGCCG GCGAACTGGA 
ACCTACGGAG GATTTTTGCT TGCCTGCTAT GACACCGAAA ACGAGGAGTA TCAGAGCATA 
TGCAAGATTG GAACTGGCTT CACTGATGAG GACCTGCAAA CGCATTCCGA GTTTTTGGGC 
AAACATGTGA CCAGCGCCGC CAAATCCTAC TACAGATACG ACCCCAGCCT GGAGCCCGAT 
CACTGGTTTG AACCGGTCCA GGTGTGGGAA GTCAAATGCG CGGATCTGTC GCTCAGTCCG 
ATCCACCGAG CAGCCATTGG CATTGTGGAC GGAGAGCGGG GCATTTCCTT GAGATTCCCT 
CGGTTCATTC GGATACGCGA CGACAAGAAT AGCGAAAACG CAACGGACGC CAATCAGGTG 
GCCCACATGT ACCAGTCGCA GGATCAGGTT AAAAATAATC AAAAGTCGAG CACACAAATG 
GAGATGGAGG ACGAGTTCTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_611843.2
CDS	85..2328
Translation	MQKSITSFFKKKSDATDSPSPPKKVPKIDAKTELPDEPHIKSESASPETKPKVEPMSVDSEEKTSPVKNVKKEPKEVDDKTTDKKVTTIGLNSTAATKEDVENYDPSADSYHPLKNAYWKDKKVTPYLALARTFQVIEETKGRLKMIDTLSNFFCSVMLVSPEDLVPSVYLSINQLAPAYEGLELGVAETTLMKAICKATGRNLAHIKSQTQLTGDLGIVAEQSRVSQRMMFQPAPLNVRDVFRKLREIAKLSGQSKMDLVYNMFVACRSSEARFFIRSLIGKLRIGIAEQSLLTALAIGLVKKNHIDDCKASKVPDVYKDEIVDTTLLLKTAYCQCPNYDIIIPAILKYDIKELQERCPMHPGMPLRPMLAQPTKGVHEVFERFGGMQITCEWKYDGERAQIHRNEKGEISIFSRNSENNTAKYPDLIARSTALLKGDVKSYIIDSEIVAWDVERKQILPFQVLSTRKRKNVDIEEIKVQVCVYIFDLLYINGTALVTKNLSERRKLLLEHFQEVEGEWKFATALDTNDIDEVQQFLEESIKGNCEGLMVKTLDEEATYEIAKRSRNWLKLKKDYLSNVGDSLDLVVIGGYKGKGRRTGTYGGFLLACYDTENEEYQSICKIGTGFTDEDLQTHSEFLGKHVTSAAKSYYRYDPSLEPDHWFEPVQVWEVKCADLSLSPIHRAAIGIVDGERGISLRFPRFIRIRDDKNSENATDANQVAHMYQSQDQVKNNQKSSTQMEMEDEFY

Target ORF information:

RefSeq Version	NM_137999.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster DNA ligase 1 (DNAlig1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_137999.3

ORF Insert Sequence:

ATGCAAAAGT CTATAACCTC CTTCTTCAAG AAGAAATCCG ATGCCACCGA CAGCCCCTCG 
CCGCCCAAGA AAGTTCCTAA GATTGATGCG AAGACCGAAC TCCCAGATGA ACCCCACATC 
AAGAGCGAAA GTGCCTCCCC GGAAACTAAG CCCAAGGTAG AACCTATGTC TGTGGATTCC 
GAGGAGAAGA CAAGTCCGGT GAAAAATGTT AAGAAGGAAC CAAAGGAAGT CGATGACAAG 
ACCACTGACA AGAAGGTCAC AACCATAGGT CTCAACTCCA CTGCCGCTAC TAAGGAGGAT 
GTGGAAAACT ACGATCCATC CGCCGACTCC TATCATCCGC TCAAGAATGC CTACTGGAAG 
GACAAAAAGG TCACTCCCTA CTTGGCACTG GCCCGCACAT TTCAAGTAAT CGAGGAGACC 
AAAGGCCGGC TCAAGATGAT CGACACGCTG AGCAACTTCT TCTGCTCTGT GATGCTGGTC 
AGTCCGGAGG ATCTTGTGCC CAGCGTCTAC CTCAGCATCA ACCAACTGGC GCCCGCCTAC 
GAGGGACTGG AGCTGGGCGT TGCCGAGACC ACCTTGATGA AGGCCATTTG CAAGGCAACG 
GGGCGTAATT TGGCTCACAT TAAATCGCAG ACGCAGCTAA CCGGGGATCT GGGCATCGTG 
GCCGAGCAGT CGCGCGTTTC ACAGCGCATG ATGTTCCAGC CAGCGCCTCT GAATGTGAGG 
GATGTGTTCC GGAAGCTACG CGAAATAGCC AAGCTATCCG GTCAGTCGAA AATGGACCTG 
GTGTACAATA TGTTTGTGGC CTGTCGATCG TCGGAGGCTC GCTTCTTTAT CCGTTCATTG 
ATTGGCAAGC TAAGAATTGG CATTGCAGAG CAGAGTTTGC TCACAGCGCT GGCCATTGGT 
TTGGTGAAAA AGAACCACAT CGATGATTGC AAGGCTAGCA AGGTGCCGGA TGTGTATAAG 
GATGAAATAG TCGACACCAC TTTGCTGTTG AAAACTGCAT ACTGTCAGTG TCCCAACTAC 
GATATCATTA TACCGGCCAT TCTAAAGTAC GATATTAAGG AGCTGCAGGA ACGCTGCCCC 
ATGCATCCCG GCATGCCGCT GAGGCCCATG CTCGCCCAGC CCACCAAGGG GGTCCATGAG 
GTATTTGAGA GATTCGGCGG CATGCAAATC ACCTGCGAGT GGAAATACGA TGGAGAGCGG 
GCCCAAATCC ACCGCAACGA GAAGGGGGAG ATCAGCATAT TCTCGCGCAA CTCCGAGAAC 
AACACGGCAA AATATCCGGA TTTGATTGCC AGGAGCACTG CCTTGCTCAA GGGCGACGTA 
AAGTCCTACA TCATAGACAG CGAAATTGTG GCCTGGGATG TGGAGCGCAA GCAGATTCTG 
CCGTTTCAGG TGCTCAGCAC GCGCAAACGG AAGAACGTTG ATATCGAGGA AATCAAGGTC 
CAAGTCTGTG TCTACATCTT CGATCTGCTG TACATCAATG GCACTGCTCT GGTGACCAAA 
AACCTGTCGG AGCGTCGCAA GCTCCTGTTG GAGCACTTTC AAGAGGTCGA GGGCGAGTGG 
AAGTTCGCCA CTGCTCTGGA CACCAATGAC ATAGACGAAG TGCAGCAGTT CCTGGAGGAG 
TCTATCAAAG GAAATTGCGA GGGCCTCATG GTCAAAACAT TGGATGAGGA GGCCACCTAT 
GAGATTGCCA AGAGATCCCG CAACTGGCTG AAATTGAAGA AGGACTACCT ATCCAATGTG 
GGCGACTCAC TCGATTTGGT GGTCATTGGT GGCTACAAAG GCAAGGGCCG GCGAACTGGA 
ACCTACGGAG GATTTTTGCT TGCCTGCTAT GACACCGAAA ACGAGGAGTA TCAGAGCATA 
TGCAAGATTG GAACTGGCTT CACTGATGAG GACCTGCAAA CGCATTCCGA GTTTTTGGGC 
AAACATGTGA CCAGCGCCGC CAAATCCTAC TACAGATACG ACCCCAGCCT GGAGCCCGAT 
CACTGGTTTG AACCGGTCCA GGTGTGGGAA GTCAAATGCG CGGATCTGTC GCTCAGTCCG 
ATCCACCGAG CAGCCATTGG CATTGTGGAC GGAGAGCGGG GCATTTCCTT GAGATTCCCT 
CGGTTCATTC GGATACGCGA CGACAAGAAT AGCGAAAACG CAACGGACGC CAATCAGGTG 
GCCCACATGT ACCAGTCGCA GGATCAGGTT AAAAATAATC AAAAGTCGAG CACACAAATG 
GAGATGGAGG ACGAGTTCTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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DNAlig1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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